Researcher Database

ryohei kinoshita
Faculty of Veterinary Medicine Veterinary Teaching Hospital
Specially Appointed Assistant Professor

Researcher Profile and Settings

Affiliation

  • Faculty of Veterinary Medicine Veterinary Teaching Hospital

Job Title

  • Specially Appointed Assistant Professor

Degree

  • DVM(Kagoshima University)

J-Global ID

Academic & Professional Experience

  • 2019/07 - Today Hokkaido University Veterinary Teaching Hospital
  • 2014/10 - 2019/06 The University of Tokyo Veterinary Medical Center
  • 2012/04 - 2014/09 The University of Tokyo Veterinary Medical Center

Education

  • 2004/04 - 2010/03  Kagoshima University  Faculty of Agriculture  Veterinary Medicine

Research Activities

Published Papers

  • M. Shinada, K. Saeki, R. Yoshitake, S. Eto, M. Tsuboi, J.K. Chambers, K. Uchida, D. Kato, S. Yoshimoto, S. Kamoto, N. Ikeda, R. Kinoshita, N. Fujita, R. Nishimura, T. Nakagawa
    The Veterinary Journal 105571 - 105571 1090-0233 2020/11
  • Sho Yoshimoto, Daiki Kato, Satoshi Kamoto, Kie Yamamoto, Masaya Tsuboi, Masahiro Shinada, Namiko Ikeda, Yuiko Tanaka, Ryohei Yoshitake, Shotaro Eto, Kohei Saeki, James Kenn Chambers, Ryohei Kinoshita, Kazuyuki Uchida, Ryohei Nishimura, Takayuki Nakagawa
    The Journal of veterinary medical science 81 (7) 1034 - 1039 0916-7250 2019/07/19 [Refereed][Not invited]
     
    Canine anal sac gland carcinoma (ASGC) frequently occurs in the apocrine glands of the canine anal sac and shows aggressive biological behavior. The expression of human epidermal growth factor receptor 2 (HER2) has been reported in various human and canine tumors. HER2 is a promising therapeutic target of these tumors, and HER2-targeted drugs, such as trastuzumab and lapatinib, have improved the outcome of these patients. In this study, HER2 expression in ASGC was evaluated to investigate its potential as a therapeutic target for canine ASGC. HER2 mRNA expression in surgically resected ASGC tissues was significantly higher than that in normal anal sac tissue. To evaluate the expression of HER2 protein, paraffin-embedded ASGC tissues were immunohistochemically evaluated. Strong and broad staining of HER2 was detected in ASGC tissues, while HER2 was weakly to moderately stained in normal anal sac apocrine glands and squamous epithelia. The degree of HER2 expression in ASGC tissues was scored based on its intensity and positivity (score: 0-3+). Scoring of HER2 expression revealed 6 samples (24%) scored 3+, 14 (56%) scored 2+, and 5 (20%) scored 1+, with no samples scoring 0. In all, 80% of canine ASGC tissues were positive for HER2 (scored ≥2+). Furthermore, putative HER2-overexpressed cells in ASGC were detected with trastuzumab by flow cytometry. These preliminary data may lead to further evaluation of the role of HER2 in canine ASGC as a mechanism of malignancy and as a therapeutic target for HER2-targeted therapy.
  • Sho Yoshimoto, Daiki Kato, Satoshi Kamoto, Kie Yamamoto, Masaya Tsuboi, Masahiro Shinada, Namiko Ikeda, Yuiko Tanaka, Ryohei Yoshitake, Shotaro Eto, Kohei Saeki, James Chambers, Ryohei Kinoshita, Kazuyuki Uchida, Ryohei Nishimura, Takayuki Nakagawa
    Heliyon 5 (7) e02004  2019/07 [Refereed][Not invited]
     
    The human epidermal growth factor receptor 2 (HER2) is expressed in various human cancers including thyroid cancers (TC) and is used as a diagnostic marker and therapeutic target. Canine TC (cTC), the most common endocrine malignancy in dogs, shows a high metastasis rate, and HER2-targeted therapy could be a candidate for treatment. Here, we immunohistochemically evaluated HER2 expression in 21 paraffin-embedded cTC tissues and scored the degree of expression based on intensity and positivity (score: 0-3+). Four samples (19%) scored 3+; 6 (29%), 2+; 7 (33%), 1+; and 4 (19%), 0. Therefore, 48% of the cTC tissues were HER2 positive (scored ≥2+). These data may lead to further evaluation of the role of HER2 in cTC as a mechanism of malignancy and a therapeutic target.
  • 心筋症に伴う血栓塞栓症による消化管壊死が疑われた猫1例
    石川 恭平, 中川 泰輔, 水野 壮司, 山本 貴恵, 木之下 怜平, 長久保 大, 坪井 誠也, チェンバーズ・ジェームズ, 内田 和幸, 大野 耕一, 辻本 元
    日本獣医麻酔外科学雑誌 (一社)日本獣医麻酔外科学会 50 (Suppl.1) 325 - 325 2189-6623 2019/06
  • Evaluation of immunohistochemical staining with PMab-38, an anti-dog podoplanin monoclonal antibody, in various canine tumor tissues.
    Kiname K, Yoshimoto S, Kato D, Tsuboi M, Tanaka Y, Yoshitake R, Eto S, Shinada M, Chambers J, Saeki K, Kinoshita R, Yamada S, Uchida K, K. Kaneko M, Nishimura R, Kato Y, Nakagawa T
    Japanese Journal of Veterinary Research 67 (1) 25 - 34 2019/02 [Refereed][Not invited]
  • Isao Matsumoto, James K Chambers, Kazumi Nibe, Ryohei Kinoshita, Ryohei Nishimura, Hiroyuki Nakayama, Kazuyuki Uchida
    Veterinary pathology 55 (5) 663 - 672 0300-9858 2018/09 [Refereed][Not invited]
     
    The biological behavior and immunohistochemical features of feline renal cell carcinoma (RCC) have not been well characterized. In the present study, immunohistochemical examinations were performed in 12 feline cases of RCC. The RCC consisted of solid ( n = 2), solid-tubular ( n = 2), tubular ( n = 3), papillary ( n = 2), tubulopapillary ( n = 2), and sarcomatoid ( n = 1) type lesions. Of the cases with RCC, 1 developed metastatic disease and 6 cases had no evidence of recurrence at 80 to 2292 days after surgery. One papillary-type tumor had cuboidal cells with scant cytoplasm and monomorphic nuclei, and the other had pseudostratified columnar cells with abundant cytoplasm. Immunohistochemistry revealed that the tumor cells in most cases were positive for cytokeratin (CK)7, CK20, KIT, and CD10, with the exception of cases of the solid type with clear cytoplasm (solid anaplastic), papillary type with columnar cells, and sarcomatoid types. A small number of tumor cells in the solid anaplastic and in the sarcomatoid types were positive for aquaporin-1. Increased expression of N-cadherin and Twist along with nuclear accumulation of β-catenin were observed in the sarcomatoid type. These results indicated that CK, KIT, and CD10 are relatively strongly expressed in most feline RCC. The solid anaplastic RCC exhibited CD10 expression with the absence of distal tubule marker expression. Although immunohistochemistry profiles were relatively consistent with those described in human RCC, the histopathologic features were different from those seen in humans. Epithelial-mesenchymal transition (EMT) marker expression in the current cases may suggest the involvement of an EMT-like mechanism in the development of sarcomatoid RCC in cats.
  • 膀胱移行上皮癌の犬78例における治療成績の回顧的検討
    愛宕 哲也, 佐伯 亘平, 木之下 怜平, 藤原 玲奈, 坪井 誠也, 中川 貴之, 西村 亮平
    日本獣医麻酔外科学雑誌 (一社)日本獣医麻酔外科学会 49 (Suppl.1) 285 - 285 2189-6623 2018/06 [Refereed][Not invited]
  • Akiko Inoue, Shingo Maeda, Ryohei Kinoshita, Masaya Tsuboi, Tomohiro Yonezawa, Naoaki Matsuki
    Veterinary immunology and immunopathology 190 53 - 56 0165-2427 2017/08 [Refereed][Not invited]
     
    Although tumor-infiltrating lymphocytes (TILs) play a key role in anti-tumor immunity, their involvement in canine transitional cell carcinoma (TCC) is not well-documented. The objective of this study was to investigate the association between TIL number and prognosis in dogs with urinary bladder TCC. Immunohistochemical analysis of CD3 and granzyme B was performed using canine TCC (n=32) and normal bladder (n=10) tissues. The numbers of CD3+ and granzyme B+ cells located in peritumoral stroma of canine TCC were significantly higher than those in normal controls. In TCC cases, the number of CD3+ TILs was not significantly related to prognosis, whereas the abundant granzyme B+ TILs were associated with favorable outcome. Since granzyme B+ TILs were not associated with the tumor stage, the presence of granzyme B+ TILs may be an independent prognostic factor. These results suggest that granzyme B+ TILs play a role in anti-tumor immunity and inhibit tumor progression in canine TCC.
  • 犬における原発性腎腫瘍の回顧的検討
    木之下 怜平, 佐伯 亘平, 坪井 誠也, 藤原 玲奈, 西村 亮平
    獣医麻酔外科学雑誌 (一社)日本獣医麻酔外科学会 45 (Suppl.2) 188 - 188 0916-5908 2014/12 [Refereed][Not invited]
  • 犬における下部尿路移行上皮癌の回顧的検討(2005〜2011)
    木之下 怜平, 佐伯 亘平, 坪井 誠也, 中川 貴之, 西村 亮平
    獣医麻酔外科学雑誌 (一社)日本獣医麻酔外科学会 44 (Suppl.2) 244 - 244 0916-5908 2013/12 [Refereed][Not invited]

Books etc

  • 大野, 耕一 (Joint work必須手術手技)
    EDUWARD Press 2021/06 (ISBN: 9784866711539) x, 393p
  • Rodríguez Gómez, José, Llinás, Jorge, Bussadori, Roberto, 西村, 亮平 (気管)
    ファームプレス 2020/11 (ISBN: 9784863821118) xix, 241p
  • 辻本, 元, 小山, 秀一, 大草, 潔, 中村, 篤史 (胸腺腫 耳垢腺癌)
    Eduward press 2020/08 (ISBN: 9784866711225) xvi, 963, 23p
  • 辻本, 元, 小山, 秀一, 大草, 潔, 中村, 篤史 (Contributor胸腺腫 耳垢腺癌)
    Eduward press 2020/08 (ISBN: 9784866711218) xvi, 1219, 28p
  • Day Brühl, Rodolfo, Martínez, María Elena, Meyer, Pablo, Rodríguez Gómez, José, 西村, 亮平 (尿路手術の過誤)
    ファームプレス 2019/10 (ISBN: 9784863821071) xiii, 223p
  • 木之下怜平, 他多数 (Contributor組織・切除生検法)
    インターズー 2018/09 (ISBN: 9784866710303) 323p ; 挿図

Conference Activities & Talks

  • はじめての会陰ヘルニア「筋肉フラップを用いた整復法」  [Invited]
    木之下怜平
    獣医麻酔外科学会 北海道地区講習会  2021/02
  • 獣医学での植皮術  [Invited]
    木之下怜平
    第97回日本獣医麻酔外科学会  2019/01
  • Short- and medium-term outcome of ureterotomy and neoureterocystostomy with active abdominal drainage in cats with obstructive ureterolithiasis: 15cases, 23ureters.  [Not invited]
    木之下怜平
    The 8th Annual AiSVS Congress  2018/12
  • 犬の尿管閉塞 私の解除法  [Invited]
    木之下怜平
    日本獣医麻酔外科学会東京地区講習会  2018/03
  • 尿路のツマリの解除法  [Invited]
    木之下怜平
    日本獣医内科学アカデミー学術大会  2017/02

Awards & Honors

  • 2014/12 Japanese Society of Veterinary Anesthesia and Surgery Award of soft tissue surgery
     犬における原発性腎腫瘍の回顧的検討 
    受賞者: 木之下怜平;佐伯亘平;坪井誠也;藤原玲奈;西村亮平

Research Grants & Projects

  • Novel therapeutic strategy for canine urothelial carcinoma: cancer elimination by anti-inflammation with BRAF inhibitor
    Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
    Date (from‐to) : 2019/04 -2021/03 
    Author : 木之下 怜平

Educational Activities

Teaching Experience

  • Practice in Companion Animal Medicine I
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 伴侶動物、内科、外科、検査、診断、治療
  • Practice in Companion Animal Medicine II
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 伴侶動物、内科、外科、検査、診断、治療
  • Practice in Comprehensive Veterinary Medicine
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 臨床病理、画像診断、細胞診、病理解剖、麻酔
  • Practice in Night Times and Emergency Medicine I
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 夜間・休日診療、救急処置


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