薬学博士, 九州大学

Molecular Imaging

Metabolism

pathophysiology

Neuroscience

Hyperpolarized 13C MRI

Physical Chemistry

Radiology

Quantum Chemistry

Radiation Biology

Nuclear Hyperpolarization

Electron Spin Resonance

Life Science, Pharmaceutical analytical chemistry and physicochemistry, 分子イメージング

Life Science, Physiology

Life Science, Cognitive and brain science

Informatics, Life, health and medical informatics, 生体情報科学

Life Science, Tumor diagnostics and therapeutics

Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering), Measurement engineering

Life Science, Medical systems

Bachelor's degree program, School of Engineering

Master's degree program, Graduate School of Information Science and Technology

Doctoral (PhD) degree program, Graduate School of Information Science and Technology

2017, 日本応用酵素協会, 酵素研究奨励賞

2017, 豊田理研, 豊田理研スカラー

May 2015, International Society for Magnetic Resonance in Medicine (ISMRM), Distinguished Reviewer Award of Magnetic Resonance in Medicine

Sep. 2013, World Molecular Imaging Congress (WMIC), Top Abstract

Sep. 2012, World Molecular Imaging Congress (WMIC), Top Abstract

2008, World Molecular Imaging Congress, Travel Stipend Award
MATSUMOTO Shingo;NCI;NIH

2008, EPR2008, Young Investigator Award
MATSUMOTO Shingo;NCI;NIH

Tolerance to extracellular acidic pH facilitates tumor plasticity.
Manami Hasegawa; Bo Xu; Keisuke Maeda; Motoaki Seki; FeiFei Cai; Runmei Cui; Ritsuko Ando; Suzuka Nakagawa; Ayana Sakamoto; Cayla Boycott; Hiroyuki Yatabe; Miyuki Nishida; Ken Matsumoto; Chisato Iwabuchi-Yoshida; Sho Aki; Kazuyuki Yamagata; Rika Tsuchida; Mami Takahashi; Futoshi Kuribayashi; Hiroyasu Kidoya; Hiroshi Hirata; Shingo Matsumoto; Shinsuke Sando; Hideyuki Yanai; Nozomu Yachie; Tsuyoshi Osawa
Cell Reports, 117226, 117226, 16 Apr. 2026, [International Magazine]
English, Scientific journal, Cancer cells sustain glycolysis despite oxygen availability, creating an acidic microenvironment via proton and lactate export, but how they survive acid stress is unclear. We show that severe acidification (pH 5.6) induces necroptosis, whereas moderate acidity (pH 6.8) prevents death and enables anchorage-independent survival and tumor initiation. RNA sequencing of suspended cells at pH 6.8 revealed activation of respiratory chain complex and complement pathways, consistent with adaptation to this pH. A genome-wide CRISPR-Cas9 knockout screen in PANC1 cells under chronic acidity identified FAM129C as a regulator of acid tolerance and survival. In xenografts, FAM129C overexpression reduced PIGR expression, implicating this axis in tumor growth and immune infiltration. Anti-PD-L1 plus a complement inhibitor showed synergistic anti-tumor activity in PIGR-overexpressing tumors. Thus, acidic stress engages a pathway that allows cancer cells to evade necroptosis and promote tumor plasticity, providing potential avenues for therapeutic intervention targeting pH-dependent cell-death pathways.

Random Acquisition of Spectral Projections in Four-Dimensional Spectral-Spatial EPR Imaging: A Feasibility Study Toward Oxygen Imaging
Misa Oba; Mai Taguchi; Shingo Matsumoto; Hiroshi Hirata
Advances in Experimental Medicine and Biology, 59, 64, Springer Nature Switzerland, 24 Jan. 2026
In book

Non-invasive visualisation of long-lasting brain metabolic alterations in murine pseudo-infection model using parahydrogen-polarised [1-13C] pyruvate MRI.
Hayate Tomiyama; Masaki Yamasaki; Takayuki Isagawa; Norihiko Takeda; Takuya Hashimoto; Hiroshi Hirata; Shingo Matsumoto
Discover nano, 20, 1, 128, 128, 06 Aug. 2025, [Peer-reviewed], [Invited], [Corresponding author], [International Magazine]
English, Scientific journal, Long-lasting neurological issues, including cognitive impairment, anxiety, and depression, that persist after recovery from acute inflammatory diseases, such as infections, have become a significant social problem, particularly following the coronavirus disease 2019 pandemic. Various diagnostic techniques and biomarkers have been explored to objectively evaluate brain symptoms associated with infection-induced local or systemic inflammatory responses (i.e. immune fatigue); however, their detection capabilities remain limited. Here we investigated whether magnetic resonance imaging (MRI) combined with a quantum-sensed molecule, parahydrogen-polarised [1-13C] pyruvate, could detect persistent brain metabolic alterations in a murine pseudo-infection model induced by polyinosinic-polycytidylic acid (Poly(I: C)), a Toll-like receptor 3 ligand. Significant alterations in brain pyruvate metabolism favouring glycolysis were observed in both the acute and late phases of the pseudo-infection model, with a 12.7% and 2.5% decrease in bicarbonate flux, and a 58.4% and 32.2% increase in lactate flux on day 3 and week 2, respectively. These brain metabolic changes were accompanied by diminished dopamine signal markers in the striatum and nigra/ventral tegmental areas and reduced spontaneous nocturnal locomotor activity. A biochemical analysis of energy metabolic markers consistently supported the reprogramming of brain glucose metabolism, showing the suppression of oxidative phosphorylation during the acute phase and promotion of glycolysis during the late phase of Poly(I: C) treatment. Hyperpolarised 13C MRI of pyruvate metabolism is a promising non-invasive imaging biomarker for brain issues during the late phase of systemic infections and other neurodegenerative and psychiatric diseases, particularly in conditions lacking discernible morphological abnormalities.

Three-dimensional electron paramagnetic resonance (EPR) imaging of an X-ray-irradiated bovine tooth: A feasibility study
Yukihiro Arai; Mai Taguchi; Oki Mitarai; Ichiro Yamaguchi; Hideo Sato-Akaba; Shingo Matsumoto; Minoru Miyake; Hiroshi Hirata
Radiation Measurements, 181, 107381, Feb. 2025, [Peer-reviewed]
Scientific journal

Partial Acquisition of Spectral Projections Accelerates Four-dimensional Spectral-spatial EPR Imaging for Mouse Tumor Models: A Feasibility Study
Misa Oba; Mai Taguchi; Yohei Kudo; Koya Yamashita; Hironobu Yasui; Shingo Matsumoto; Igor A. Kirilyuk; Osamu Inanami; Hiroshi Hirata
Molecular Imaging and Biology, 26, 3, 459, 472, Springer Science and Business Media LLC, 29 Jun. 2024, [Peer-reviewed]
Scientific journal

Parahydrogen-induced ¹³C hyperpolarizer using a flow guide for magnetic field cycling to evoke ¹H-¹³C spin order transfer toward metabolic MRI
Koudai Sawami; Tatsuya Naganuma; Hana Yabe; Toshihiko Taki; Neil J. Stewart; Yoshiki Uchio; Norihiko Takeda; Noriyuki Hatae; Takuya Hashimoto; Hiroshi Hirata; Shingo Matsumoto
IEEE Transactions on Biomedical Engineering, 71, 7, 2224, 2231, Institute of Electrical and Electronics Engineers (IEEE), 2024, [Peer-reviewed], [Corresponding author]
Scientific journal, 25978752;43651179

Electron Paramagnetic Resonance Implemented with Multiple Harmonic Detections Successfully Maps Extracellular pH In Vivo
Ririko Nakaoka; Kazuhiro Kato; Kumiko Yamamoto; Hironobu Yasui; Shingo Matsumoto; Igor A. Kirilyuk; Valery V. Khramtsov; Osamu Inanami; Hiroshi Hirata
Analytical Chemistry, American Chemical Society (ACS), 01 Feb. 2023
Scientific journal

Continuous monitoring of post-irradiation reoxygenation and cycling hypoxia using electron paramagnetic resonance imaging.
Tatsuya Kawai; Masayuki Matsuo; Yoichi Takakusagi; Keita Saito; Fuminori Hyodo; Nallathamby Devasahayam; Shingo Matsumoto; Shun Kishimoto; Hironobu Yasui; Kazutoshi Yamamoto; Murali C Krishna
NMR in biomedicine, 35, 10, e4783, 03 Jun. 2022, [International Magazine]
English, Scientific journal, BACKGROUND: Reoxygenation has a significant impact on the tumor response to radiotherapy. With the developments in radiotherapy technology, the relevance of the reoxygenation phenomenon in treatment efficacy has been a topic of interest. Evaluating the reoxygenation in the tumor microenvironment throughout the course of radiation therapy is important in developing effective treatment strategies. PURPOSE: In the present study, we used electron paramagnetic resonance imaging (EPRI) to directly map and quantify the partial oxygen pressure (pO2 ) in tumor tissues. MATERIALS AND METHODS: Human colorectal cancer cell lines, HT29 and HCT116, were used to induce tumor growth in female athymic nude mice. Tumors were irradiated with 3, 10, or 20 Gy using an X-ray irradiator. Prior to each EPRI scan, magnetic resonance imaging (MRI) was performed to obtain T2-weighted anatomical images for reference. The differences in the mean pO2 were determined through two-tailed Student's t-test and one-way analysis of variance (ANOVA). RESULTS: The median pO2 60 min after irradiation was found to be lower in HCT116 than in HT29 (9.1 ± 1.5 vs. 14.0 ± 1.0 mmHg, P = 0.045). There was a tendency for delayed and incomplete recovery of pO2 in the HT29 tumor when a higher dose of irradiation (10 and 20 Gy) was applied. Moreover, there was a dose-dependent increase in the hypoxic areas (pO2 < 10 mmHg) 2 h and 24 h after irradiation in all groups. In addition, an area that showed pO2 fluctuation between hypoxia and normoxia (pO2 > 10 mmHg) was also identified surrounding the region with stable hypoxia, and it slightly enlarged after recovery from acute hypoxia. CONCLUSION: We demonstrated the reoxygenation phenomenon in an in vivo xenograft model study using EPRI. These findings might lead to new knowledge of the reoxygenation process and possibilities of a new radiation therapy concept, namely, reoxygenation-based radiation therapy.

Hyperpolarized 13C Magnetic Resonance Imaging as a Tool for Imaging Tissue Redox State, Oxidative Stress, Inflammation, and Cellular Metabolism
Stewart NJ; Sato T; Takeda N; Hirata H; Matsumoto S
Antioxid Redox Signal, 36, 1-3, 81, 94, Jan. 2022, [Peer-reviewed], [Invited], [Corresponding author]
25978752;39502478

Redox-sensitive mapping of a mouse tumor model using sparse projection sampling of electron paramagnetic resonance
Kimura K; Iguchi N; Nakano H; Yasui H; Matsumoto S; Inanami O; Hirata H
Antioxid Redox Signal, 36, 1-3, 57, 69, Mary Ann Liebert Inc, Jan. 2022, [Peer-reviewed]
Scientific journal

Synthesis of [1-13C-5-12C]-alpha-ketoglutarate enables noninvasive detection of 2-hydroxyglutarate.
Miura N; Mushti C; Sail D; AbuSalim JE; Yamamoto K; Brender JR; Seki T; AbuSalim DI; Matsumoto S; Camphausen KA; Krishna MC; Swenson RE; Kesarwala AH
NMR Biomed, 34, 11, e4588, Nov. 2021, [Peer-reviewed]

Biomedical Applications of the Dynamic Nuclear Polarization and Parahydrogen Induced Polarization Techniques for Hyperpolarized ¹³C MR Imaging
Stewart NJ; Matsumoto S
Magn Reson Med Sci, 20, 1, 1, 17, Japanese Society for Magnetic Resonance in Medicine, May 2021, [Peer-reviewed], [Invited], [Corresponding author]
Scientific journal

Hyperpolarized 13 C Magnetic Resonance Imaging of Fumarate Metabolism by Parahydrogen-induced Polarization: A Proof-of-Concept in vivo Study
Stewart NJ; Nakano H; Sugai S; Tomohiro M; Kase Y; Uchio Y; Yamaguchi T; Matsuo Y; Naganuma T; Takeda N; Nishimura I; Hirata H; Hashimoto T; Matsumoto S
ChemPhysChem, 22, 10, 915, 923, Wiley, Feb. 2021, [Peer-reviewed], [Invited], [Corresponding author]
Scientific journal, 25978752;12176393;12176406

Macrophage derived TNFα promotes hepatic reprogramming to Warburg-like metabolism.
Tarasenko TN; Jestin M; Matsumoto S; Saito K; Hwang S; Gavrilova O; Trivedi N; Zerfas PM; Barca E; DiMauro S; Senac J; Venditti CP; Cherukuri M; McGuire PJ
J Mol Med, 97, 9, 1231, 1243, Sep. 2019, [Peer-reviewed]

Imaging of glucose metabolism by 13C-MRI distinguishes pancreatic cancer subtypes in mice.
Kishimoto S; Brender JR; Crooks DR; Matsumoto S; Seki T; Oshima N; Merkle H; Lin P; Reed G; Chen AP; Ardenkjaer-Larsen JH; Munasinghe J; Saito K; Yamamoto K; Choyke PL; Mitchell J; Lane AN; Fan TW; Linehan WM; Krishna MC
eLIFE, 8, e46312, Aug. 2019, [Peer-reviewed]

Synthesis and evaluation of 13C-labeled 5-5-dimethyl-1-pyrroline-N-oxide aimed at in vivo detection of reactive oxygen species using hyperpolarized 13C-MRI.
Saito K; Sail D; Yamamoto K; Matsumoto S; Blackman B; Kishimoto S; Brender JR; Swenson RE; Mitchell JB; Krishna MC
Free Radic Biol Med, 1, 131, 18, 26, Feb. 2019, [Peer-reviewed]

Simultaneous T2^* mapping of ¹⁴N- and ¹⁵N-labeled dicarboxy-PROXYLs using CW-EPR-based single-point imaging
Yokoyama, T.; Taguchi, A.; Kubota, H.; Stewart, N.J.; Matsumoto, S.; Kirilyuk, I.A.; Hirata, H.
Journal of Magnetic Resonance, 305, 2019, [Peer-reviewed]
Scientific journal

Proteasome inhibition disrupts the metabolism of fumarate hydratase- deficient tumors by downregulating p62 and c-Myc
Sourbier, C.; Ricketts, C.J.; Liao, P.-J.; Matsumoto, S.; Wei, D.; Lang, M.; Railkar, R.; Yang, Y.; Wei, M.-H.; Agarwal, P.; Krishna, M.; Mitchell, J.B.; Trepel, J.B.; Neckers, L.; Linehan, W.M.
Scientific Reports, 9, 1, 2019, [Peer-reviewed]
Scientific journal

Long-range heteronuclear J-coupling constants in esters: Implications for ¹³C metabolic MRI by side-arm parahydrogen-induced polarization
Neil J. Stewart; Hiroyuki Kumeta; Mitsushi Tomohiro; Takuya Hashimoto; Noriyuki Hatae; Shingo Matsumoto
Journal of Magnetic Resonance, 296, 85, 92, Nov. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal

In Vivo Extracellular pH Mapping of Tumors Using Electron Paramagnetic Resonance.
Komarov DA; Ichikawa Y; Yamamoto K; Stewart NJ; Matsumoto S; Yasui H; Kirilyuk IA; Khramtsov VV; Inanami O; Hirata H
Anal Chem, Nov. 2018, [Peer-reviewed]

Pulsed Electron Paramagnetic Resonance Imaging: Applications in the Studies of Tumor Physiology.
Kishimoto S; Matsumoto KI; Saito K; Enomoto A; Matsumoto S; Mitchell JB; Devasahayam N; Krishna MC
Antioxid Redox Signal, 28, 15, 1378, 1393, Nov. 2018, [Peer-reviewed]

EPR-based oximetric imaging: a combination of single point-based spatial encoding and T1 weighting.
Ken-Ichiro Matsumoto; Shun Kishimoto; Nallathamby Devasahayam; Gadisetti V R Chandramouli; Yukihiro Ogawa; Shingo Matsumoto; Murali C Krishna; Sankaran Subramanian
Magnetic resonance in medicine, 80, 5, 2275, 2287, Nov. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, PURPOSE: Spin-lattice relaxation rate (R1 )-based time-domain EPR oximetry is reported for in vivo applications using a paramagnetic probe, a trityl-based Oxo71. METHODS: The R1 dependence of the trityl probe Oxo71 on partial oxygen pressure (pO2 ) was assessed using single-point imaging mode of spatial encoding combined with rapid repetition, similar to T1 -weighted MRI, for which R1 was determined from 22 repetition times ranging from 2.1 to 40.0 μs at 300 MHz. The pO2 maps of a phantom with 3 tubes containing 2 mM Oxo71 solutions equilibrated at 0%, 2%, and 5% oxygen were determined by R1 and apparent spin-spin relaxation rate ( R2*) simultaneously. RESULTS: The pO2 maps derived from R1 and R2* agreed with the known pO2 levels in the tubes of Oxo71. However, the histograms of pO2 revealed that R1 offers better pO2 resolution than R2* in low pO2 regions. The SDs of pixels at 2% pO2 (15.2 mmHg) were about 5 times lower in R1 -based estimation than R2*-based estimation (mean ± SD: 13.9 ± 1.77 mmHg and 18.3 ± 8.70 mmHg, respectively). The in vivo pO2 map obtained from R1 -based assessment displayed a homogeneous profile in low pO2 regions in tumor xenografts, consistent with previous reports on R2*-based oximetric imaging. The scan time to obtain the R1 map can be significantly reduced using 3 repetition times ranging from 4.0 to 12.0 μs. CONCLUSION: Using the single-point imaging modality, R1 -based oximetry imaging with useful spatial and oxygen resolutions for small animals was demonstrated.

Impact of the characteristic impedance of coaxial lines on the sensitivity of a 750-MHz electronically tunable EPR resonator
R. Nakaoka; D. A. Komarov; S. Matsumoto; H. Hirata
Applied Magnetic Resonance, 49, 8, 853, 867, Springer Science and Business Media LLC, Aug. 2018, [Peer-reviewed]
English, Scientific journal

Metabolic and Physiologic Imaging Biomarkers of the Tumor Microenvironment Predict Treatment Outcome with Radiation or a Hypoxia-Activated Prodrug in Mice.
Matsumoto S; Kishimoto S; Saito K; Takakusagi Y; Munasinghe JP; Devasahayam N; Hart CP; Gillies RJ; Mitchell JB; Krishna MC
Cancer Res., 78, 14, 3783, 3792, Jul. 2018, [Peer-reviewed], [Lead author]
English, Scientific journal

Pulsed Electron Paramagnetic Resonance Imaging: Applications in the Studies of Tumor Physiology.
Shun Kishimoto; Ken-Ichiro Matsumoto; Keita Saito; Ayano Enomoto; Shingo Matsumoto; James B Mitchell; Nallathamby Devasahayam; Murali C Krishna
Antioxidants & redox signaling, 28, 15, 1378, 1393, 20 May 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, SIGNIFICANCE: Electron paramagnetic resonance imaging (EPRI) is capable of generating images of tissue oxygenation using exogenous paramagnetic probes such as trityl radicals or nitroxyl radicals. The spatial distribution of the paramagnetic probe can be generated using magnetic field gradients as in magnetic resonance imaging and, from its spectral features, spatial maps of oxygen can be obtained from live objects. In this review, two methods of signal acquisition and image formation/reconstruction are described. The probes used and its application to study tumor physiology and monitor treatment response with chemotherapy drugs in mouse models of human cancer are summarized. Recent Advances: By implementing phase encoding/Fourier reconstruction in EPRI in time domain mode, the frequency contribution to the spatial resolution was avoided and images with improved spatial resolution were obtained. The EPRI-generated pO2 maps in tumor were useful to detect and evaluate the effects of various antitumor therapies on tumor physiology. Coregistration with other imaging modalities provided a better understanding of hypoxia-related alteration in physiology. CRITICAL ISSUES: The high radiofrequency (RF) power of EPR irradiation and toxicity profile of radical probes are the main obstacles for clinical application. The improvement of RF low power pulse sequences may allow for clinical translation. FUTURE DIRECTIONS: Pulsed EPR oximetry can be a powerful tool to research various diseases involving hypoxia such as cancer, ischemic heart diseases, stroke, and diabetes. With appropriate paramagnetic probes, it can also be applied for various other purposes such as detecting local acid-base balance or oxidative stress. Antioxid. Redox Signal. 28, 1378-1393.

A 750-MHz Electronically Tunable Resonator Using Microstrip Line Couplers for Electron Paramagnetic Resonance Imaging of a Mouse Tumor-Bearing Leg.
Tatsuya Amida; Ririko Nakaoka; Denis A Komarov; Kumiko Yamamoto; Osamu Inanami; Shingo Matsumoto; Hiroshi Hirata
IEEE transactions on bio-medical engineering, 65, 5, 1124, 1132, May 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: The purpose of this work was to develop an electronically tunable resonator operating at 750 MHz for continuous-wave electron paramagnetic resonance (CW-EPR) imaging of a mouse tumor-bearing leg. METHODS: The resonator had a multi-coil parallel-gap structure with a sample space of 16 mm in diameter and 20 mm in length. Microstrip line couplers were used in conjunction with varactor diodes to enable resonance frequency adjustment and to reduce the nonlinear effects of the varactor diodes. The resonator was modeled by the finite-element method and a microwave circuit simulation was performed to clarify its radiofrequency characteristics. RESULTS: A tunable resonator was evaluated in terms of its resonance frequency, tunable frequency band, and conversion efficiency of the RF magnetic field. The developed resonator provided a tunable frequency band of 4 MHz at a central frequency of 747 MHz and a conversion efficiency of 34 μT/W1/2. To demonstrate the application of this tunable resonator to EPR imaging, three-dimensional EPR images of a sample solution and a mouse tumor-bearing leg were obtained. CONCLUSION: The developed tunable resonator satisfied our initial requirements for in vivo EPR imaging and may be able to be further improved using the present finite-element and circuit models if any problems arise during future practical applications. SIGNIFICANCE: This work may help to promote EPR imaging of tumor-bearing mice in cancer-related studies.

Radiotherapy Synergizes with the Hypoxia-Activated Prodrug Evofosfamide: In Vitro and In Vivo Studies
Yoichi Takakusagi; Shun Kishimoto; Sarwat Naz; Shingo Matsumoto; Keita Saito; Charles P. Hart; James B. Mitchell; Murali C. Krishna
ANTIOXIDANTS & REDOX SIGNALING, 28, 2, 131, 140, Jan. 2018, [Peer-reviewed]
English, Scientific journal

Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging
Harue Kubota; Denis A. Komarov; Hironobu Yasui; Shingo Matsumoto; Osamu Inanami; Igor A. Kirilyuk; Valery V. Khramtsov; Hiroshi Hirata
MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE, 30, 3, 291, 298, Jun. 2017, [Peer-reviewed]
English, Scientific journal

Effect of amifostine, a radiation-protecting drug, on oxygen concentration in tissue measured by EPR oximetry and imaging
Megumi Ueno; Shingo Matsumoto; Atsuko Matsumoto; Sushma Manda; Ikuo Nakanishi; Ken-ichiro Matsumoto; James B. Mitchell; Murali C. Krishna; Kazunori Anzai
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 60, 3, 151, 155, May 2017, [Peer-reviewed]
English, Scientific journal

Quantitative imaging of pO(2) in orthotopic murine gliomas: hypoxia correlates with resistance to radiation
Hironobu Yasui; Tatsuya Kawai; Shingo Matsumoto; Keita Saito; Nallathamby Devasahayam; James B. Mitchell; Kevin Camphausen; Osamu Inanami; Murali C. Krishna
FREE RADICAL RESEARCH, 51, 9-10, 861, 871, 2017, [Peer-reviewed]
English, Scientific journal

C-13-MR Spectroscopic Imaging with Hyperpolarized [1-C-13]pyruvate Detects Early Response to Radiotherapy in SCC Tumors and HT-29 Tumors
Keita Saito; Shingo Matsumoto; Yoichi Takakusagi; Masayuki Matsuo; H. Douglas Morris; Martin J. Lizak; Jeeva P. Munasinghe; Nallathamby Devasahayam; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna
CLINICAL CANCER RESEARCH, 21, 22, 5073, 5081, Nov. 2015, [Peer-reviewed]
English, Scientific journal

Accelerated 4D Quantitative Single Point EPR Imaging Using Model-Based Reconstruction
Hyungseok Jang; Shingo Matsumoto; Nallathamby Devasahayam; Sankaran Subramanian; Jiachen Zhuo; Murali C. Krishna; Alan B. McMillan
MAGNETIC RESONANCE IN MEDICINE, 73, 4, 1692, 1701, Apr. 2015, [Peer-reviewed]
English, Scientific journal

Pyruvate sensitizes pancreatic tumors to hypoxia-activated prodrug TH-302
Wojtkowiak JW; Cornnell HC; Matsumoto S; Saito K; Takakusagi Y; Dutta P; Kim M; Zhang X; Leos R; Bailey KM; Martinez G; Lloyd MC; Weber C; Mitchell JB; Lynch RM; Baker AF; Gatenby RA; Rejniak KA; Hart C; Krishna MC; Gillies RJ
Cancer Metab, 3, 1, 2, Jan. 2015, [Peer-reviewed]
English, Scientific journal

Targeting ABL1-Mediated Oxidative Stress Adaptation in Fumarate Hydratase-Deficient Cancer
Carole Sourbier; Christopher J. Ricketts; Shingo Matsumoto; Daniel R. Crooks; Pei-Jyun Liao; Philip Z. Mannes; Youfeng Yang; Ming-Hui Wei; Gaurav Srivastava; Sanchari Ghosh; Viola Chen; Cathy D. Vocke; Maria Merino; Ramaprasad Srinivasan; Murali C. Krishna; James B. Mitchell; Ann Marie Pendergast; Tracey A. Rouault; Len Neckers; W. Marston Linehan
CANCER CELL, 26, 6, 840, 850, Dec. 2014, [Peer-reviewed]
English, Scientific journal

Pyruvate Induces Transient Tumor Hypoxia by Enhancing Mitochondrial Oxygen Consumption and Potentiates the Anti-Tumor Effect of a Hypoxia-Activated Prodrug TH-302
Yoichi Takakusagi; Shingo Matsumoto; Keita Saito; Masayuki Matsuo; Shun Kishimoto; Jonathan W. Wojtkowiak; William DeGraff; Aparna H. Kesarwala; Rajani Choudhuri; Nallathamby Devasahayam; Sankaran Subramanian; Jeeva P. Munasinghe; Robert J. Gillies; James B. Mitchell; Charles P. Hart; Murali C. Krishna
PLOS ONE, 9, 9, e107995, Sep. 2014, [Peer-reviewed]
English, Scientific journal

In Vivo Imaging of Tumor Physiological, Metabolic, and Redox Changes in Response to the Anti-Angiogenic Agent Sunitinib: Longitudinal Assessment to Identify Transient Vascular Renormalization
Shingo Matsumoto; Keita Saito; Yoichi Takakusagi; Masayuki Matsuo; Jeeva P. Munasinghe; Herman D. Morris; Martin J. Lizak; Hellmut Merkle; Keiji Yasukawa; Nallathamby Devasahayam; Sankaran Suburamanian; James B. Mitchell; Murali C. Krishna
ANTIOXIDANTS & REDOX SIGNALING, 21, 8, 1145, 1155, Sep. 2014, [Peer-reviewed], [Lead author]
English, Scientific journal

Magnetic Resonance Imaging of the Tumor Microenvironment in Radiotherapy: Perfusion, Hypoxia, and Metabolism
Masayuki Matsuo; Shingo Matsumoto; James B. Mitchell; Murali C. Krishna; Kevin Camphausen
SEMINARS IN RADIATION ONCOLOGY, 24, 3, 210, 217, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Four-Channel Surface Coil Array for 300-MHz Pulsed EPR Imaging: Proof-of-Concept Experiments
Ayano Enomoto; Hiroshi Hirata; Shingo Matsumoto; Keita Saito; Sankaran Subramanian; Murali C. Krishna; Nallathamby Devasahayam
MAGNETIC RESONANCE IN MEDICINE, 71, 2, 853, 858, Feb. 2014, [Peer-reviewed]
English, Scientific journal

Single Acquisition Quantitative Single-Point Electron Paramagnetic Resonance Imaging
Hyungseok Jang; Sankaran Subramanian; Nallathamby Devasahayam; Keita Saito; Shingo Matsumoto; Murali C. Krishna; Alan B. McMillan
MAGNETIC RESONANCE IN MEDICINE, 70, 4, 1173, 1181, Oct. 2013, [Peer-reviewed]
English, Scientific journal

Magnetic resonance imaging of tumor oxygenation and metabolic profile
Murali C. Krishna; Shingo Matsumoto; Keita Saito; Masayuki Matsuo; James B. Mitchell; Jan H. Ardenkjaer-Larsen
ACTA ONCOLOGICA, 52, 7, 1248, 1256, Oct. 2013, [Peer-reviewed]
English

Evaluation of partial k-space strategies to speed up time-domain EPR imaging
Sankaran Subramanian; Gadisetti V. R. Chandramouli; Alan McMillan; Rao P. Gullapalli; Nallathamby Devasahayam; James B. Mitchell; Shingo Matsumoto; Murali C. Krishna
MAGNETIC RESONANCE IN MEDICINE, 70, 3, 745, 753, Sep. 2013, [Peer-reviewed]
English, Scientific journal

EPR Oxygen Imaging and Hyperpolarized C-13 MRI of Pyruvate Metabolism as Noninvasive Biomarkers of Tumor Treatment Response to a Glycolysis Inhibitor 3-Bromopyruvate
Shingo Matsumoto; Keita Saito; Hironobu Yasui; H. Douglas Morris; Jeeva P. Munasinghe; Martin Lizak; Hellmut Merkle; Jan Henrik Ardenkjaer-Larsen; Rajani Choudhuri; Nallathamby Devasahayam; Sankaran Subramanian; Alan P. Koretsky; James B. Mitchell; Murali C. Krishna
MAGNETIC RESONANCE IN MEDICINE, 69, 5, 1443, 1450, May 2013, [Peer-reviewed], [Lead author]
English, Scientific journal

The relationship between tissue oxygenation and redox status using magnetic resonance imaging
Fuminori Hyodo; Ryan M. Davis; Emi Hyodo; Shingo Matsumoto; Murali C. Krishna; James B. Mitchell
INTERNATIONAL JOURNAL OF ONCOLOGY, 41, 6, 2103, 2108, Dec. 2012, [Peer-reviewed]
English, Scientific journal

Longitudinal Imaging Studies of Tumor Microenvironment in Mice Treated with the mTOR Inhibitor Rapamycin
Keita Saito; Shingo Matsumoto; Hironobu Yasui; Nallathamby Devasahayam; Sankaran Subramanian; Jeeva P. Munasinghe; Vyomesh Patel; J. Silvio Gutkind; James B. Mitchell; Murali C. Krishna
PLOS ONE, 7, 11, e49456, Nov. 2012, [Peer-reviewed]
English, Scientific journal

Echo-based Single Point Imaging (ESPI): A novel pulsed EPR imaging modality for high spatial resolution and quantitative oximetry
Sankaran Subramanian; Nallathamby Devasahayam; Shingo Matsumoto; Keita Saito; James B. Mitchell; Murali C. Krishna
JOURNAL OF MAGNETIC RESONANCE, 218, 105, 114, May 2012, [Peer-reviewed]
English, Scientific journal

Electron Paramagnetic Resonance Imaging of Tumor pO(2)
Murali C. Krishna; Shingo Matsumoto; Hironobu Yasui; Keita Saito; Nallathamby Devasahayam; Sankaran Subramanian; James B. Mitchell
RADIATION RESEARCH, 177, 4, 376, 386, Apr. 2012, [Peer-reviewed]
English, Scientific journal

Transient decrease in tumor oxygenation after intravenous administration of pyruvate
Keita Saito; Shingo Matsumoto; Nallathamby Devasahayam; Sankaran Subramanian; Jeeva P. Munasinghe; H. Douglas Morris; Martin J. Lizak; Jan Henrik Ardenkjaer-Larsen; James B. Mitchell; Murali C. Krishna
MAGNETIC RESONANCE IN MEDICINE, 67, 3, 801, 807, Mar. 2012, [Peer-reviewed]
English, Scientific journal

Reporting of quantitative oxygen mapping in EPR imaging
Sankaran Subramanian; Nallathamby Devasahayam; Alan McMillan; Shingo Matsumoto; Jeeva P. Munasinghe; Keita Saito; James B. Mitchell; Gadisetti V. R. Chandramouli; Murali C. Krishna
JOURNAL OF MAGNETIC RESONANCE, 214, 1, 244, 251, Jan. 2012, [Peer-reviewed]
English, Scientific journal

Antiangiogenic Agent Sunitinib Transiently Increases Tumor Oxygenation and Suppresses Cycling Hypoxia
Shingo Matsumoto; Sonny Batra; Keita Saito; Hironobu Yasui; Rajani Choudhuri; Chandramouli Gadisetti; Sankaran Subramanian; Nallathamby Devasahayam; Jeeva P. Munasinghe; James B. Mitchell; Murali C. Krishna
CANCER RESEARCH, 71, 20, 6350, 6359, Oct. 2011, [Peer-reviewed], [Lead author]
English, Scientific journal

Detecting Response of Rat C6 Glioma Tumors to Radiotherapy Using Hyperpolarized [1-C-13]Pyruvate and C-13 Magnetic Resonance Spectroscopic Imaging
Sam E. Day; Mikko I. Kettunen; Murali Krishna Cherukuri; James B. Mitchell; Martin J. Lizak; H. Douglas Morris; Shingo Matsumoto; Alan P. Koretsky; Kevin M. Brindle
MAGNETIC RESONANCE IN MEDICINE, 65, 2, 557, 563, Feb. 2011, [Peer-reviewed]
English, Scientific journal

Magnetic resonance imaging of organic contrast agents in mice: capturing the whole-body redox landscape
Ryan M. Davis; Shingo Matsumoto; Marcelino Bernardo; Anastasia Sowers; Ken-Ichiro Matsumoto; Murali C. Krishna; James B. Mitchell
FREE RADICAL BIOLOGY AND MEDICINE, 50, 3, 459, 468, Feb. 2011, [Peer-reviewed]
English, Scientific journal

Fatty acid amide hydrolase is a key regulator of endocannabinoid-induced myocardial tissue injury
Partha Mukhopadhyay; Bela Horvath; Mohanraj Rajesh; Shingo Matsumoto; Keita Saito; Sandor Batkai; Vivek Patel; Galin Tanchian; Rachel Y. Gao; Benjamin F. Cravatt; Gyoergy Hasko; Pal Pacher
FREE RADICAL BIOLOGY AND MEDICINE, 50, 1, 179, 195, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Intracellular Hypoxia of Tumor Tissue Estimated by Noninvasive Electron Paramagnetic Resonance Oximetry Technique Using Paramagnetic Probes
Atsuko Matsumoto; Ken-ichiro Matsumoto; Shingo Matsumoto; Fuminori Hyodo; Anastasia L. Sowers; Janusz W. Koscielniak; Nallathamby Devasahayam; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 34, 1, 142, 145, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Imaging Cycling Tumor Hypoxia
Shingo Matsumoto; Hironobu Yasui; James B. Mitchell; Murali C. Krishna
CANCER RESEARCH, 70, 24, 10019, 10023, Dec. 2010, [Peer-reviewed], [Lead author]
English

Cannabidiol Attenuates Cardiac Dysfunction, Oxidative Stress, Fibrosis, and Inflammatory and Cell Death Signaling Pathways in Diabetic Cardiomyopathy
Mohanraj Rajesh; Partha Mukhopadhyay; Sandor Batkai; Vivek Patel; Keita Saito; Shingo Matsumoto; Yoshihiro Kashiwaya; Bela Horvath; Bani Mukhopadhyay; Lauren Becker; Gyoergy Hasko; Lucas Liaudet; David A. Wink; Aristidis Veves; Raphael Mechoulam; Pal Pacher
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 56, 25, 2115, 2125, Dec. 2010, [Peer-reviewed]
English, Scientific journal

Low-Field Magnetic Resonance Imaging to Visualize Chronic and Cycling Hypoxia in Tumor-Bearing Mice
Hironobu Yasui; Shingo Matsumoto; Nallathamby Devasahayam; Jeeva P. Munasinghe; Rajani Choudhuri; Keita Saito; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna
CANCER RESEARCH, 70, 16, 6427, 6436, Aug. 2010, [Peer-reviewed]
English, Scientific journal

Simultaneous imaging of tumor oxygenation and microvascular permeability using Overhauser enhanced MRI
Shingo Matsumoto; Hironobu Yasui; Sonny Batra; Yuichi Kinoshita; Marcelino Bernardo; Jeeva P. Munasinghe; Hideo Utsumi; Rajani Choudhuri; Nallathamby Devasahayam; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106, 42, 17898, 17903, Oct. 2009, [Peer-reviewed], [Lead author]
English, Scientific journal

Estimation of tumor microvessel density by MRI using a blood pool contrast agent
Fuminori Hyodo; Gadisetti V. R. Chandramouli; Shingo Matsumoto; Ken-Ichiro Matsumoto; James B. Mitchell; Murali C. Krishna; Jeeva P. Munasinghe
INTERNATIONAL JOURNAL OF ONCOLOGY, 35, 4, 797, 804, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Pulsed EPR imaging of nitroxides in mice
Fuminori Hyodo; Shingo Matsumoto; Nallathamby Devasahayam; Christopher Dharmaraj; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna
JOURNAL OF MAGNETIC RESONANCE, 197, 2, 181, 185, Apr. 2009, [Peer-reviewed]
English, Scientific journal

Reconstruction for time-domain in vivo EPR 3D multigradient oximetric imaging--a parallel processing perspective
Dharmaraj CD; Thadikonda K; Fletcher AR; Doan PN; Devasahayam N; Matsumoto S; Johnson CA; Cook JA; Mitchell JB; Subramanian S; Krishna MC
Int J Biomed Imaging, 2009, 528639, 2009, [Peer-reviewed]

Synthesis of nitroxyl radicals for overhauser-enhanced magnetic resonance imaging
Ken-ichi Yamada; Yuichi Kinoshita; Toshihide Yamasaki; Hiromi Sadasue; Fumiya Mito; Mika Nagai; Shingo Matsumoto; Mariko Aso; Hiroshi Suemune; Kiyoshi Sakai; Hideo Utsumi
ARCHIV DER PHARMAZIE, 341, 9, 548, 553, Sep. 2008, [Peer-reviewed]
English, Scientific journal

Assessment of tissue redox status using metabolic responsive contrast agents and magnetic resonance imaging
Fuminori Hyodo; Benjamin P. Soule; Ken-Ichiro Matsumoto; Shingo Matusmoto; John A. Cook; Emi Hyodo; Anastasia L. Sowers; Murali C. Krishna; James B. Mitchell
JOURNAL OF PHARMACY AND PHARMACOLOGY, 60, 8, 1049, 1060, Aug. 2008, [Peer-reviewed]
English, Scientific journal

Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice
Matsumoto, Shingo; Hyodo, Fuminori; Subramanian, Sankaran; Devasahayam, Nallathamby; Munasinghe, Jeeva; Hyodo, Emi; Gadisetti, Chandramouli; Cook, John A.; Mitchell, James B.; Krishna, Murali C.
JOURNAL OF CLINICAL INVESTIGATION, 118, 5, 1965, 1973, May 2008, [Peer-reviewed], [Lead author]
English, Scientific journal

Dynamic monitoring of localized tumor oxygenation changes using RF pulsed electron paramagnetic resonance in conscious mice
Shingo Matsumoto; Michael Graham Espey; Hideo Utsumi; Nallathamby Devasahayam; Ken-Ichiro Matsumoto; Atsuko Matsumoto; Hiroshi Hirata; David A. Wink; Periarman Kuppusamy; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna
MAGNETIC RESONANCE IN MEDICINE, 59, 3, 619, 625, Mar. 2008, [Peer-reviewed], [Lead author]
English, Scientific journal

Advantageous application of a surface coil to EPR irradiation in Overhauser-enhanced MRI
Shingo Matsumoto; Kenichi Yamada; Hiroshi Hirata; Keiji Yasukawa; Fuminori Hyodo; Kazuhiro Ichikawa; Hideo Utsumi
MAGNETIC RESONANCE IN MEDICINE, 57, 4, 806, 811, Apr. 2007, [Peer-reviewed], [Lead author]
English, Scientific journal

Are free radical reactions increased in the diabetic eye?
Mayumi Yamato; Shingo Matsumoto; Katsuaki Ura; Ken-Ichi Yamada; Tatsuya Naganuma; Toyoshi Inoguchi; Toshiaki Watanabe; Hideo Utsumi
ANTIOXIDANTS & REDOX SIGNALING, 9, 3, 367, 373, Mar. 2007, [Peer-reviewed]
English

Simultaneous molecular imaging of redox reactions monitored by Overhauser-enhanced MRI with 14N- and 15N-labeled nitroxyl radicals
Utsumi H; Yamada K; Ichikawa K; Sakai K; Kinoshita Y; Matsumoto S; Nagai M
Proc Natl Acad Sci USA, 103, 5, 1463, 1468, 2006, [Peer-reviewed]
English, Scientific journal

Absolute oxygen tension (pO(2)) muscle tissue as determined by in murine fatty and EPR
A Matsumoto; S Matsumoto; AL Sowers; JW Koscielniak; NJ Trigg; P Kuppusamy; JB Mitchell; S Subramanian; MC Krishna; K Matsumoto
MAGNETIC RESONANCE IN MEDICINE, 54, 6, 1530, 1535, Dec. 2005, [Peer-reviewed]
English, Scientific journal

Influence of proton T-1 on oxymetry using Overhauser enhanced magnetic resonance imaging
S Matsumoto; H Utsumi; T Aravalluvan; K Matsumoto; A Matsumoto; N Devasahayam; AL Sowers; JB Mitchell; S Subramanian; MC Krishna
MAGNETIC RESONANCE IN MEDICINE, 54, 1, 213, 217, Jul. 2005, [Peer-reviewed], [Lead author]
English, Scientific journal

In vivo imaging of oxidative stress in the kidney of diabetic mice and its normalization by angiotensin II type 1 receptor blocker
T Sonta; T Inoguchi; S Matsumoto; K Yasukawa; M Inuo; H Tsubouchi; N Sonoda; K Kobayashi; H Utsumi; H Nawata
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 330, 2, 415, 422, May 2005, [Peer-reviewed]
English, Scientific journal

A composite resonator assembly suitable for EPR/NMR coregistration imaging
S Matsumoto; M Nagai; K Yamada; F Hyodo; K Yasukawa; M Muraoka; H Hirata; M Ono; H Utsumi
CONCEPTS IN MAGNETIC RESONANCE PART B-MAGNETIC RESONANCE ENGINEERING, 25B, 1, 1, 11, Apr. 2005, [Peer-reviewed], [Lead author]
English, Scientific journal

Evalution of oxidative stress in diabetic animals by in vivo electron spin resonance measurement - role of protein kinase C
T Sonta; T Inoguchi; H Tsubouchi; N Sekiguchi; K Kobayashi; S Matsumoto; H Utsumi; H Nawata
DIABETES RESEARCH AND CLINICAL PRACTICE, 66, Suppl 1, S109, S113, Dec. 2004, [Peer-reviewed]
English, Scientific journal

Evidence for contribution of vascular Nad(P)H oxidase to increased oxidative stress in animal models of diabetes and obesity
T Sonta; T Inoguchi; H Tsubouchi; N Sekiguchi; K Kobayashi; S Matsumoto; H Utsumi; H Nawata
FREE RADICAL BIOLOGY AND MEDICINE, 37, 1, 115, 123, Jul. 2004, [Peer-reviewed]
English, Scientific journal

In vivo measurement of redox status in streptozotocin-induced diabetic rat using targeted nitroxyl probes
KI Yamada; D Inoue; S Matsumoto; H Utsumi
ANTIOXIDANTS & REDOX SIGNALING, 6, 3, 605, 611, Jun. 2004, [Peer-reviewed]
English, Scientific journal

Confirmation of superoxide generation via xanthine oxidase in streptozotocin-induced diabetic mice
S Matsumoto; Koshiishi, I; T Inoguchi; H Nawata; H Utsumi
FREE RADICAL RESEARCH, 37, 7, 767, 772, Jul. 2003, [Peer-reviewed], [Lead author]
English, Scientific journal

Feasibility study of tooth irradiation dose mapping using EPR
新井之紘; 山口一郎; 松元慎吾; 赤羽英夫; 三宅実; 平田拓, 電子スピンサイエンス学会年会講演要旨集, 62nd (CD-ROM), 2023

[Imaging of Tumor-Specific Hypoxia Dynamics and Its Significance in Radiation Biology].
Hironobu Yasui; Shingo Matsumoto; Osamu Inanami; Murali Cherukuri Krishna, Igaku butsuri : Nihon Igaku Butsuri Gakkai kikanshi = Japanese journal of medical physics : an official journal of Japan Society of Medical Physics, 40, 1, 13, 18, 2020, [Domestic magazines]
Hypoxia has been known to be a feature associated with tumor radioresistance. So far, clinical strategies to overcome chronic hypoxia due to the limitation of the oxygen diffusion have been designed. However, intermittent or acute/cycling hypoxia, whose frequency can range between a few cycles per minutes to hours, is receiving increased attention, because this type of hypoxia has been reported to have an influence on tumor malignancy as well as treatment resistance via increased expression of pro-survival pathways. Therefore, a priori information on fluctuating hypoxia can be important in clinical treatment planning, but complicated dynamics makes it difficult to elucidate biological significance of intermittent hypoxia.Here, we illustrate the use of pulsed electron spin resonance imaging (ESRI) as a novel imaging method to directly monitor fluctuating oxygenation i.e. cycling hypoxia in transplanted tumors. A common resonator platform for both ESRI and magnetic resonance imaging (MRI) provided pO2 maps with anatomical guidance without positional movement. Oxygen images every 3 min in pO2 could visualize the rapid oxygen fluctuation and distinguish the cycling hypoxia and chronic hypoxia. Furthermore, we have examined the vascular renormalization process by longitudinally pO2 mapping during treatments with a multi-tyrosine kinase inhibitor sunitinib. Transient improvement in tumor oxygenation and the decrease of cycling tumor hypoxia were visualized by ESRI 2 to 4 days following antiangiogenic treatments. Radiation treatment during this time period of improved oxygenation by antiangiogenic therapy resulted in a synergistic delay in tumor growth.In conclusion, this ESRI technique combined with MRI, may offer a powerful clinical tool to noninvasively detect variable hypoxic status in tumors and to identify a window of vascular renormalization to maximize the effects of combination therapy with antiangiogenic drugs., Japanese

マルチモダル分子イメージングによる腫瘍内微小環境の多角的評価と天然由来含硫糖脂質の誘発する放射線増感作用メカニズムの解明
高草木 洋一; 松元 慎吾; 松尾 政之; 高草木 香織; 齋藤 圭太; 岸本 俊; 三浦 雅彦; 宮崎 年恭; 菅原 二三男; 坂口 謙吾; James B Mitchell; Murali C Krishna, 第 14 回 日本分子イメージング学会総会・学術集会, May 2019, [Peer-reviewed]

Application of ESR pO₂ imaging to evaluation of tumor radiosensitivity in intracranial glioma mouse model
安井博宣; 河合辰哉; 松元慎吾; 齋藤圭太; CAMPHAUSEN Kevin; KRISHNA Murali; 稲波修, 電子スピンサイエンス学会年会講演要旨集, 57th, 106‐107, 01 Nov. 2018
Japanese

Serial Imaging Analysis by both absolute partial pressure of oxygen (pO2) and hyperpolarized [1-13C] pyruvate metabolism Changes in Cancer Treatment
Masayuki Matsuo; Shingo Matsumoto; Keita Saito; Yoichi Takakusagi; Hidekazu Tanaka; Fuminori Hyodo; James B. Mitchell; Murali C. Krishna, 46th JSMRM Annual Meeting, Sep. 2018, [Peer-reviewed]
English

Monitoring effect of rapamaycin on pyruvate metabolism in SCC tumor using hyperpolarized 13C-MRI
Keita Saito; Shingo Matsumoto; Yoichi Takakusagi; Masayuki Matsuo; Hellmut Merkle; James B. Mitchell; Murali C. Krishna, International Society for Magnetic Resonance in Medicine (ISMRM) 2018, 3069, Jun. 2018, [Peer-reviewed]
English, Introduction international proceedings

最先端量子イメージングによる膵臓がん個別化医療のモデル研究
松元慎吾; 高草木洋一; 齋藤圭太; 岸本俊 Mitchell; James B. Hart; Charles P. Gillies; Robert J. Krishna; Murali C, 第 2 回 量子生命科学研究会, May 2018, [Invited]
Japanese

電子スピン共鳴酸素イメージングによる脳室内移植グリオーマの放射線感受性評価
安井博宣; 安井博宣; 河合辰哉; 松元慎吾; 松元慎吾; 齋藤圭太; CAMPHAUSEN Kevin; 稲波修; KRISHNA Murali, 日本放射線影響学会大会抄録(Web), 61st, 92 (WEB ONLY), 92, 2018
(一社)日本放射線影響学会, Japanese

Oxygen partial pressure imaging of tumor model mice with isotopically labeled nitroxyl radicals
久保田晴江; 安井博宣; 松元慎吾; 稲波修; KIRILYUK Igor; KHRAMTSOV Valery V.; 平田拓, 電子スピンサイエンス学会年会講演要旨集, 56th, 2017

腫瘍内酸素イメージングのための同位体標識ニトロキシルラジカル混合溶液の特性評価
久保田晴江; 安井博宣; 松元慎吾; 稲波修; KIRILYUK Igor; KHRAMTSOV Valery V.; 平田拓, 電子スピンサイエンス学会年会講演要旨集, 55th, 2016

酸素感受性同位体ニトロキシルラジカルの特性評価実験
久保田晴江; 安井博宣; 松元慎吾; 三宅祐輔; 稲波修; KIRILYUK I. A.; KHRAMTSOV V. V.; 平田拓, 電子スピンサイエンス学会年会講演要旨集, 54th, 2015

放射線照射における超偏極MRIおよびEPRI画像解析
松尾政之; 芝本雄太; 松元慎吾; 齋藤圭太; 高草木洋一, 第 43 回 日本磁気共鳴医学会大会, 2015
Japanese, Summary national conference

Noninvasive assessment of drug actions in tumor microenvironment by the EPR oxygen imaging
Yoichi Takakusagi; Shingo Matsumoto; Keita Saito; Masayuki Matsuo Shun; Kishimoto Kaori; Takakusagi Masahiko Miura Fumio Sugawara Kengo; Sakaguchi Robert; J. Gillies Charles P. Hart James; B. Mitchell Murali C. Krishna, 第 54 回 電子スピンサイエンス学会年会 (SEST2015), 2015
English, Summary national conference

13C-MRI with hyperpolarized [1-13C]pyruvate detects metabolic changes in SCC tumors and HT-29 tumors by radiotherapy
Keita Saito; Shingo Matsumoto; Yoichi Takakusagi; Masayuki Matsuo; H. Douglas Morris; Martin J. Lizak; Jeeva; P. Munasinghe; Nallathamby Devasahayam; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna, International Conference of Radiation Research (ICRR2015), 2015
English, Summary international conference

Increase in tumor oxygenataion and radiosensitivity caused by SQAP (CG-0321): the mechanism of actions revealed by in vivo imaging studies
Yoichi Takakusagi; Shingo Matsumoto; Keita Saito; Masayuki Matsuo; Kaori Takakusagi; Shun Kishimoto; Masahiro Ishima; Hiroshi Murata; Keisuke Ohta; Masahiko Miura; Fumio Sugawara; Kengo Sakaguchi; James B. Mitchell; Murali C. Krishna, International Conference of Radiation Research (ICRR2015), 2015
English, Summary international conference

Serial Imaging of Physiological and Metabolic Changes in Response to Radiation Therapy With Tumor-Bearing Mice
M. Matsuo; M. Shingo; S. Keita; T. Yoichi; K. Shun; D. Nallathamby; S. Sankaran; M. Douglas; M. Jeeva; M. James; K. Murali; S. Yuta, INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 90, S87, S87, Sep. 2014
English, Summary international conference

Detection of metabolic changes in SCC tumor by mTOR inhibition using hyperpolarized 13C-pyruvate MRI
Keita Saito; Shingo Matsumoto; Yoichi Takakusagi; Masayuki Matsuo; H. Douglas Morris; Hellmut Merkle; Martin J. Lizak; Jeeva; P. Munasinghe; Nallathamby Devasahayam; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna, Joint Annual Meeting ISMRM-ESMRMB 2014, 2014
English, Summary international conference

含硫糖脂質スルホキノボシルアシルグリセロール (SQAG) 誘導体による放射線増感作用: in vivo ライブイメージングによる増感機構の解明と治療後評価
高草木洋一; 松元慎吾; 齋藤圭太; 松尾政之; 高草木香織; 岸本俊 Daryl Despres Jeeva; P. Munasinghe; Nallathamby Devasahayam; 石間正浩; 村田寛; 太田慶祐; 三浦雅彦; 坂口謙吾; 菅原二三男; James B. Mitchell Murali C. Krishna, 文部科学省新学術領域研究 化学療法基盤支援活動第3回シンポジウム, 2014
Japanese, Summary national conference

EPR spectroscopy and oxygen imaging for evaluation of the transient induction of tumor hypoxia and potentiation of bioreductively activated hypoxia-activated prodrug TH-302
Yoichi Takakusagi; Shingo Matsumoto; Keita Saito; Masayuki Matsuo; Shun Kishimoto; Jonathan; W. Wojtkowiak; Robert J. Gillies; James B. Mitchell; Charles P. Hart; Murali C. Krishna, Joint Conference of APES2014・IES・SEST2014, 2014
English, Summary international conference

電子スピン共鳴法を用いた酸素イメージングによる腫瘍内間欠的低酸素の描出とその意義
安井博宣; 松元慎吾; 齋藤圭太; 山盛徹; KRISHNA Murali; 稲波修, 日本放射線影響学会大会講演要旨集, 56th, 2013

パルスEPRイメージングを用いたがん代謝標的薬剤ジクロロ酢酸処理後の腫瘍内酸素環境の経時的解析
安井博宣; 齋藤圭太; 西田直哉; 松元慎吾; 山盛徹; KRISHNA Murali C.; 稲波修, 電子スピンサイエンス学会年会講演要旨集, 52nd, 2013

Effects of rapamycin on energy metabolism, oxygenation, and angiogenesis in SCC tumor
Keita Saito; Shingo Matsumoto; Yoichi Takakusagi; Masayuki Matsuo; Jeeva; P. Munasinghe; H. Douglas Morris; Martin J. Lizak; Nallathamby Devasahayam; Sankaran Subramanian; Vyomesh Patel; Silvio J. Gutkind; James B. Mitchell; Murali C. Krishna, 41st Annual ISOTT Meeting & EPR 2013 Tul4, 2013
English, Summary international conference

EPR oxygen imaging guided treatment in pancreatic cancer
Shingo Matsumoto; Keita Saito; Yoichi Takakusagi; Masayuki Matsuo; Jeeva; P. Munasinghe; Nallathamby Devasahayam; Sankaran Subramanian; James B. Mitchell; Murali C. Krishna, World Molecular Imaging Congress, 2013
English, Summary international conference

Imaging-guided determination of the treatment regimens of the hypoxia-activated prodrug TH-302
Yoichi Takakusagi; Shingo Matsumoto; Keita Saito; Masayuki Matsuo; William DeGraff; Rajani Choudhuri; Nallathamby Devasahayam; Sankaran Subramanian, Jeeva; P. Munasinghe; James B. Mitchell; Charles P. Hart; Murali C. Krishna, World Biotechnology Congress 2013, 2013
English, Summary international conference

Chronic and cyclic hypoxia in fractionated radiation therapy
Masayuki Matsuo; Shingo Matsumoto; Keita Saito; Yoichi Takakusagi; H. Douglas; Morris, Jeeva; P. Munasinghe; Nallathamby Devasahayam; Sankallan Subramanian; James B. Mitchell; Murali C. Krishna, ASTRO Annual Meeting, 2012
English, Summary international conference

腫瘍組織に特徴的な間欠的低酸素のイメージングと放射線抵抗性への関与
安井博宣; 松元慎吾; 新坊弦也; KRISHNA Murali; 山盛徹; MITCHELL James; 稲波修, 日本放射線影響学会大会講演要旨集, 54th, 2011

パルスESRイメージングによる腫瘍内酸素濃度変動の非侵襲的解析
安井博宣; 安井博宣; 松元慎吾; MITCHELL James B.; KRISHNA Murali C., 日本酸化ストレス学会学術集会プログラム・抄録集, 63rd, 2010

移植腫瘍内における異なる低酸素状態の非侵襲的可視化
安井博宣; 安井博宣; 松元慎吾; DEAVASAHAYAM Nallathamby; SUBRAMANIAN Sankaran; MITCHELL Jamea; KRISHNA Murali, 日本獣医学会学術集会講演要旨集, 150th, 2010

腫瘍血管の不完全性に由来する間欠的低酸素状態の非侵襲的解析
安井博宣; 安井博宣; 松元慎吾; MITCHELL James; KRISHNA Murali, 日本放射線影響学会大会講演要旨集, 53rd, 2010

パルスEPR法を用いた固形腫瘍内における周期的低酸素の非侵襲的イメージング
安井博宣; 安井博宣; 松元慎吾; DEAVASAHAYAM Nallathamby; SUBRAMANIAN Sankaran; MITCHELL James B.; KRISHNA Murali C., 電子スピンサイエンス学会年会講演要旨集, 48th, 2009

バイオイメージング特論, 2024年, 修士課程, 情報科学院

バイオイメージング特論, 2024年, 博士後期課程, 情報科学研究科

バイオイメージング特論, 2024年, 博士後期課程, 情報科学院

一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育

生体医工学基礎, 2024年, 学士課程, 工学部

生体工学概論, 2024年, 学士課程, 工学部

生体情報工学実験Ⅰ, 2024年, 学士課程, 工学部

応用数学Ⅰ, 2024年, 学士課程, 工学部

電子回路, 2024年, 学士課程, 工学部

Jun. 2022 - Present
日本医学物理学会

2020 - Present
The Parahydrogen Enhanced Resonance Meeting PERM

2017 - Present
International Society of Magnetic Resonance

Apr. 2015 - Present
日本分子イメージング学会

生物個体内のライブpH計測・可視化技術の開発
科学研究費助成事業
01 Apr. 2025 - 31 Mar. 2030
松元 慎吾
日本学術振興会, 学術変革領域研究(A), 北海道大学, 25H01337

超偏極13C脳代謝MRIによる末梢・全身性疾患に伴う認知機能障害の包括的理解
科学研究費助成事業
Apr. 2024 - Mar. 2028
松元 慎吾, 土井久志
日本学術振興会, 基盤研究(A), 北海道大学, 24H00786

一重項水素を用いた脳代謝フラックスの超高感度センシングとストレス応答解析
革新的先端研究開発支援事業PRIME
Oct. 2023 - Mar. 2027
松元慎吾
日本医療研究開発機構AMED, 北海道大学, Principal investigator, 23828544

Development of parahydrogen-polarized 13C MRI of infectious sequelae.
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Pioneering)
Jun. 2022 - Mar. 2025
Shingo Matsumoto
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Pioneering), Hokkaido University, Principal investigator, 22K18435

常温・低磁場核偏極による安定同位体PETイメージングの実現と実用展開
科学研究費補助金 基盤研究(A) 20H00654
Apr. 2020 - Mar. 2023
松元慎吾; 武田憲彦
超偏極タグは、安定同位体である13Cや15Nで標識した分子の核偏極率（= MRI感度に比例）を数万倍に励起することにより、放射性同位体標識に匹敵する高感度検出を実現する新しい分子センシング技術である。本研究では、１）水素ガスを用いた常温・低磁場核偏極により、現行の動的核偏極型の10分の1の低コストで導入可能な安定同位体標識によるPET様の分子イメージング技術（SI-PET）を実現する。このSI-PETを疾患モデルに適用し、２）心筋梗塞における心筋虚血の検出、代謝変容を指標とする認知症の早期検出、炎症性疾患における細胞死イメージング、の３つの診断法を確立することを目的とする。
２年度目となる令和３年度は、初年度に構築した超偏極13C注射剤の全製造プロセスをマイコンPSoCにより自動制御するシステムを用いて励起装置を最適化することで、[1-13C]ピルビン酸の製造量30mL以上および13C偏極率7.5%を達成した。この30mLの反応系を４セット並列駆動することで、臨床応用に必要な100mL以上の超偏極13C注射剤の製造が期待される成果が得られた。励起装置の詳細とその応用研究として、常温低磁場核偏極により生成した超偏極13Cフマル酸を用いて、肝障害モデルにおける細胞死イメージングのin vivo撮像に成功した成果を合わせて学術雑誌への投稿準備を進めている。また、超偏極13C MRIの高解像度化を目指しDixon-IDEAL型の撮像シーケンスと再構成プログラムの開発を進め、数値ファントムを用いたシミュレーションでは、空間分解能1mmで４代謝物を区別した同時イメージングが可能であることを確認した。今後は上記の励起装置とMRI撮像技術を疾患モデル動物に適用し、改善した画質でのより実用的な代謝イメージング取得を目指す。
日本学術振興機構, 基盤研究(A), 北海道大学, Principal investigator, 20H00654

On site metabolome analysis using ordinal temp and low-field nuclear polarization and deeplearning
Grants-in-Aid for Scientific Research Challenging Research (Exploratory)
Jul. 2020 - Mar. 2022
Matsumoto Shingo
We have developed a high-speed NMR-based metabolome analysis system that collectively polarized metabolites obtained by biopsy and measured by increasing the NMR signals by tens of thousands of times using ordinal temperature and low magnetic field nuclear polarization technology. The 13C NMR signal in the 1.5T NMR apparatus was significantly increased up to 70,000 times, and metabolites with a concentration of several tens of μM could be detected by 13C NMR. On the other hand, due to the delay caused by equipment failure in the first year, the identification of disease-specific metabolic changes by LC/TOF-MS remained in the group of known disease marker metabolites. We constructed an automated system using an embedded microcomputer for all processes from sample injection, hyperpolarization induction, polarization transfer, and post-process to the metabolites containing derivatized carboxylic acid by the flow method.
Japan Society for the Promotion of Science, Challenging Research (Exploratory), Hokkaido University, 20K21873

Development of a method of 3D oxygen and pH mapping of tumors using electron spin resonance spectroscopy
Grants-in-Aid for Scientific Research
Apr. 2019 - Mar. 2022
Hirata Hiroshi
We aimed to develop quantitative imaging techniques for the partial pressure of oxygen and extracellular pH for malignant tumors. In this study, electron spin resonance (ESR) spectroscopy was applied to the three-dimensional mapping of the partial pressure of oxygen and pH in solution samples. Using a molecular probe that can concurrently detect the partial pressure of oxygen and pH, we showed the feasibility of concurrent mapping the partial pressure of oxygen and pH in solution samples.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 19H02146

低磁場核偏極による生体分子の超高感度センシング技術の開発
AMED Sentankeisoku
Aug. 2018 - Mar. 2021
MATSUMOTO Shingo
Japan Agency for Medical Research and Development, Principal investigator, Competitive research funding

パラ水素誘起核偏極によるピルビン酸の超偏極１３Ｃ ＭＲＩ代謝イメージング法の発展
科学研究費助成事業
Jul. 2018 - Mar. 2020
松元 慎吾; STEWART NEIL
超偏極13C核磁気共鳴画像（Magnetic Resonance Imaging, MRI）は炭素の安定同位体である13Cで標識した化合物の13C NMR/MRI信号を一時的に数万倍に励起することで、その生体内における代謝反応をリアルタイムに可視化する最先端のMRI技術である。様々な臓器のがん診断や心機能評価において、欧米では既に数百人規模の臨床試験が行われ、その有用性が実証されている。その反面、現行の動的核偏極（Dynamic Nuclear Polarization, DNP）型の13C励起装置による臨床初期コストは3-4億円と非常に高額であり、本技術の国内普及を妨げる主因となっている。受入研究者・松元は、この超偏極13C MRIの臨床コストを10分の1に抑制すること目指し、水素ガスと量子技術を組み合わせたパラ水素誘起分極（PHIP）法による13C励起技術の研究開発を行っている。
特別研究員は昨年度までに、超偏極13C MRIの臨床応用において、最も重要な代謝プローブであるがPHIP法では通常励起できないピルビン酸を励起するためのサイドアーム型PHIP励起装置を構築した。NMR技術を駆使して決定したピルビン酸前駆体分子の全てのJ結合定数から、量子統計力学シミュレーションにより13C励起条件を最適化し、励起プロセスに実装することで、最終的に1.5Tの熱平衡状態と比較してピルビン酸の13C MRI信号の４万倍励起を達成した。2年度目は当研究室の他の大学院生と共に、スパースサンプリングされた部分的な13C MRI画像データから、人工知能技術の１つである深層学習とテンソル分解によるノイズ除去を組み合わせて画像を再構成する高速撮像法の研究開発も進め、代謝イメージングの画質を落とすことなく、時間分解能を約5倍向上することに成功した。
日本学術振興会, 特別研究員奨励費, 北海道大学, 18F18114

パラ水素誘起分極13C MRIによる細胞死の非侵襲的イメージング技術の開発
JSPS Houga
Jul. 2018 - Mar. 2020
MATSUMOTO Shingo
JSPS, Principal investigator, Competitive research funding

Imaging of acquired mutations using hydrogen energy
JSPS Young Investigator A
Apr. 2016 - Mar. 2020
MATSUMOTO Shingo
JSPS, Principal investigator, Competitive research funding

Model experiments of partial pressure of oxygen-guided intensity modulated radiation therapy
Grants-in-Aid for Scientific Research
Apr. 2016 - Mar. 2018
Hirata Hiroshi; MATSUMOTO Shingo
The purpose of this study was to clarify the outcomes of the model experiments of oxygen partial pressure-guided radiation therapy. A miniature multi-leaf collimator (MLC) was developed for the model experiments using tumor-bearing mice. This miniature MLC was planned to control the intensity and the distribution of X-ray irradiation to tumor model mice. Also, the computation method for obtaining the collimator opening from the three-dimensional data of oxygen partial pressure in mouse tumors was developed and implemented. Moreover, tumor-growth curves were obtained for non-treated and uniform X-ray irradiated mice. These data will be the control data of tumor growth. For preparing tumor-bearing mice, murine squamous cell carcinoma (SCC VII) cells were subcutaneously implanted in mouse hind legs.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 16K12862

Dynamic monitoring of homeostasis disruption using hyperpolarized 13C MRI
PRESTO
Feb. 2015 - Mar. 2018
MATSUMOTO Shingo
Japan Science and Technology Agency, Principal investigator, Competitive research funding

Intensity Standard for L-band EPR Tooth Dosimetry
Pilot Project
Dec. 2013 - Dec. 2014
MATSUMOTO Shingo
Dart-CMCR, Principal investigator, Competitive research funding

ESRI・MRI融合型画像解析システムの開発と臨床応用に関する研究
JSPS DC2
Apr. 2004 - Mar. 2006
MATSUMOTO Shingo
Japan Society for The Promotion of Science, Principal investigator, Competitive research funding

Transient hypoxia inducers and their use
Patent right, Shingo Matsumoto; Keita Satito; Murali C. Krishna; James B. Mitchell
特願61/478,465, 22 Apr. 2011
特開US2012/034534, 20 Apr. 2012

Quantitative oxygen imaging systems and methods using echo-based single point imaging
Patent right, Sankaran Suramanian; Nallathamby Devasahayam; Shingo Matsumoto; James B. Mitchell; Cherukuri M. Krishna; John A. cook
特願13/131,165, 25 Nov. 2009
特開US2009/065956, 25 Nov. 2009
特表WO2010/062973, 03 Jun. 2010