SEARCH

Search Details

Ando Yasuhiro

Faculty of Fisheries Sciences　Marine Life Science　Marine Bioresources ChemistryAssociate Professor

Researcher basic information

■ Degree
  • 博士（水産学), 北海道大学
  • 水産学修士, 北海道大学
■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research Keyword
  • HPLC
  • Gas chromatography
  • Lipid analysis
  • 水産脂質
  • Chemistry and Biochemistry on Marine Lipids (6301
Research Field
  • Life Science, Aquatic life science
■ Educational Organization

Career

■ Career
Career
  • 2005 - 2007
    大学院水産科学研究院海洋生命科学部門生物資源化学分野 助教授
  • 2005 - 2007
    Associate Professor
  • 2007
    - 大学院水産科学研究院海洋生命科学部門生物資源化学分野 准教授
  • 2000 - 2005
    大学院水産科学研究科生命資源科学専攻生物資源化学講座 助教授
  • 2000 - 2005
    Associate Professor
  • 1999 - 2000
    水産学部海洋生物資源化学科生物機能化学講座 助教授
  • 1999 - 2000
    Associate Professor
  • 1995 - 1999
    水産学部海洋生物資源化学科生物機能化学講座 助手
  • 1995 - 1999
    Research Associate
  • 1994 - 1995
    Hokkaido University, School of Fisheries Sciences
  • 1994 - 1995
    Research Associate
  • 1993 - 1994
    日本学術振興会特別研究員 日本学術振興会特別研究員
  • 1993 - 1994
    Postdoctoral Fellowships of Japan Society for the Promotion of Science
Educational Background
  • 1993, Hokkaido University, 水産学研究科, 水産化学専攻, Japan
  • 1993, Hokkaido University, Graduate School, Division of Fisheries
  • 1990, Hokkaido University, 水産学研究科, 水産化学専攻, Japan
  • 1990, Hokkaido University, Graduate School, Division of Fisheries
  • 1988, Hokkaido University, School of Fisheries Sciences, 水産化学科, Japan
  • 1988, Hokkaido University, Faculty of Fisheries

Research activity information

■ Papers
  • Characterization of the fatty acid composition of Pacific bluefin tuna Thunnus orientalis larvae by body part and depending on growth rate
    Yuko Hiraoka; Hiroshi Hashimoto; Tetsuya Takatsu; Yuki Koide; Yosuke Tanaka; Yasuhiro Ando
    Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 111897, 111897, Elsevier BV, Jun. 2025, [Peer-reviewed], [Last author]
    Scientific journal, 34025677
  • Hydrogenimonas leucolamina sp. nov., a hydrogen-and sulphur-oxidizing mesophilic chemolithoautotroph isolated from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Western Pacific Ocean
    Shuya Hatakeyama; Sayaka Mino; Mana Mizobata; Mako Takada; Jiro Tsuchiya; Shogo Yamaki; Yasuhiro Ando; Tomoo Sawabe; Ken Takai
    International Journal of Systematic and Evolutionary Microbiology, 74, 10, 01 Oct. 2024, [Peer-reviewed]
    Scientific journal
  • The Description of Pseudoalteromonas apostichopi sp. nov., Vibrio apostichopi sp. nov., and Marinobacter apostichopi sp. nov. from the Fertilized Eggs and Larvae of Apostichopus japonicus.
    Rika Kudo; Ryota Yamano; Juanwen Yu; Shuya Hatakeyama; Chunqi Jiang; Sayaka Mino; Shogo Yamaki; Yasuhiro Ando; Yuichi Sakai; Tomoo Sawabe
    Current microbiology, 81, 8, 246, 246, 28 Jun. 2024, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Three novel bacterial strains, FE4T, FE10T, and LA51T, which are phylogenetically affiliated to the genera Pseudoalteromonas, Vibrio, or Marinobacter, respectively, isolated from fertilized eggs and juveniles of sea cucumber Apostichopus japonicus were characterized by a genome-based taxonomical approach including multilocus sequence analysis (MLSA) combined with classical phenotypic and chemotaxonomic characterizations. A molecular network reconstructed on the basis of nucleotide sequences of four phylogenetic maker protein genes revealed that the strains FE4T, FE10T, and LA51T were closely related to Pseudoalteromonas shioyasakiensis, Vibrio lentus, and Marinobacter similis, respectively. Average nucleotide identity (ANI) comparisons against phylogenetically related species to FE4T, FE10T, and LA51T demonstrated that each newly described strain could not be identified as any previously described species within each genus showing < 95% ANI: 91.3% of FE4T against P. shioyasakiensis JCM 18891 T, 92.6% of FE10T against "V. bathopelagicus" Sal10, and 92.6% of LA51T against M. similis A3d10T, in maximum, respectively. Here, we show molecular phylogenetic, genomic, phenotypic, and chemotaxonomic features of the newly described species FE4T, FE10T, and LA51T. We also propose Pseudoalteromonas apostichopi sp. nov. with FE4T (JCM 36173 T = LMG 33143 T) as the type strain, Vibrio apostichopi sp. nov. with FE10T (JCM 36174 T = LMG 33144 T) as the type strain, and Marinobacter apostichopi sp. nov. with LA51T (JCM 36175 T = LMG 33145 T) as the type strain.
  • Geographical and species differences of fatty acid components of small pelagic fishes, micronekton, and squids in the northwestern pacific
    Seiji Ohshimo; Yuko Hiraoka; Yasuhiro Ando
    Aquaculture and Fisheries, Elsevier BV, Nov. 2023, [Peer-reviewed]
    Scientific journal
  • Hydrogenimonas cancrithermarum sp. nov., a hydrogen- and thiosulfate-oxidizing mesophilic chemolithoautotroph isolated from diffuse-flow fluids on the East Pacific Rise, and an emended description of the genus Hydrogenimonas.
    Sayaka Mino; So Fukazawa; Jiro Tsuchiya; Jesse C McNichol; Stefan M Sievert; Shogo Yamaki; Yasuhiro Ando; Tomoo Sawabe
    International journal of systematic and evolutionary microbiology, 73, 11, Nov. 2023, [Peer-reviewed], [International Magazine]
    English, Scientific journal, A novel mesophilic, hydrogen- and thiosulfate-oxidizing bacterium, strain ISO32T, was isolated from diffuse-flow hydrothermal fluids from the Crab Spa vent on the East Pacific Rise. Cells of ISO32T were rods, being motile by means of a single polar flagellum. The isolate grew at a temperature range between 30 and 55 °C (optimum, 43 °C), at a pH range between 5.3 and 7.6 (optimum, pH 5.8) and in the presence of 2.0-4.0 % NaCl (optimum, 2.5 %). The isolate was able to grow chemolithoautotrophically with molecular hydrogen, thiosulfate or elemental sulfur as the sole electron donor. Thiosulfate, elemental sulfur, nitrate and molecular oxygen were each used as a sole electron acceptor. Phylogenetic analysis of 16S rRNA gene sequences placed ISO32T in the genus Hydrogenimonas of the class Epsilonproteobacteria, with Hydrogenimonas thermophila EP1-55-1 %T as its closest relative (95.95 % similarity). On the basis of the phylogenetic, physiological and genomic characteristics, it is proposed that the organism represents a novel species within the genus Hydrogenimonas, Hydrogenimonas cancrithermarum sp. nov. The type strain is ISO32T (=JCM 39185T =KCTC 25252T). Furthermore, the genomic properties of members of the genus Hydrogenimonas are distinguished from those of members of other thermophilic genera in the orders Campylobacterales (Nitratiruptor and Nitrosophilus) and Nautiliales (Caminibacter, Nautilia and Lebetimonas), with larger genome sizes and lower 16S rRNA G+C content values. Comprehensive metabolic comparisons based on genomes revealed that genes responsible for the Pta-AckA pathway were observed exclusively in members of mesophilic genera in the order Campylobacterales and of the genus Hydrogenimonas. Our results indicate that the genus Hydrogenimonas contributes to elucidating the evolutionary history of Epsilonproteobacteria in terms of metabolism and transition from a thermophilic to a mesophilic lifestyle.
  • Geographic variation in population structure and grazing features of Calanus glacialis/marshallae in the Pacific Arctic Ocean
    Minami Ishihara; Kohei Matsuno; Koki Tokuhiro; Yasuhiro Ando; Kazutoshi Sato; Atsushi Yamaguchi
    Frontiers in Marine Science, 10, Frontiers Media SA, 20 Sep. 2023, [Peer-reviewed]
    Scientific journal, Calanus glacialis/marshallae is a dominant zooplankton species in the Pacific Arctic Ocean that is widely distributed in shelf areas, and it plays a vital role in connecting primary production to higher trophic levels. Its phenology is well adapted to hydrography, but there is little available information about regional and diel changes in population structure and grazing features. In this study, we investigated C. glacialis/marshallae during autumn 2019 in the Eastern and Northeastern Chukchi and Canadian basins to reveal geographic and diel variations in population structure, body size, grazing activity, and fatty acid composition. The abundance of C. glacialis/marshallae was found to be high on the slopes and low on the shelves. Body size (prosome length) was well described by the Bělehrádek equation combined with in-situ temperature throughout the sampling region. Cluster analyses based on hydrographic parameters were divided into four regions: southern shelf, northern shelf, slope, and basin. The southern shelf was dominated by copepodite stage five (C5) transported from the Bering Sea by Pacific waters. C4 and C5 were dominant on the northern shelf, suggesting that they grew slower than those on the southern shelf, and the populations also exhibited higher concentrations of fatty acids originating from dinoflagellates than those originating from the pan-Arctic Ocean, indicating low productivity in the region. The population on the slope had the highest abundance, C4 was dominant, and large amounts of diatom-derived eicosapentaenoic acid (EPA). These features are attributed to the upwelling of populations and nutrients that support diatom growth. In the basin, the early copepodite stages of composition were distinctly higher than those recorded in previous studies, because larger amounts of organisms flow into the region, resulting in more extended reproduction periods. In the basin, small and large forms of C5 were simultaneously found, and the small form exhibited a diel grazing activity pattern, but the large forms did not. These findings suggest their well adaptation in changing of the Pacific Arctic Ocean.
  • Geographical differences in stable isotope ratios and fatty acid and lipid signatures of chub mackerel, Scomber japonicus, in waters around Japan
    Seiji Ohshimo; Yuko Hiraoka; Satoshi Suyama; Toshihiro Tsuji; Ryuji Yukami; Tohya Yasuda; Yasuhiro Ando
    Bulletin of Marine Science, 98, 3, 247, 270, Bulletin of Marine Science, 01 Jul. 2022, [Peer-reviewed]
    Scientific journal, We analyzed stable isotope ratios, fatty acid, and lipid components of chub mackerel, Scomber japonicus, to evaluate their applicability as chemical biomarkers. Chub mackerel were collected in the East China Sea, the Sea of Japan, and the Pacific Ocean, with body sizes ranging from 51 to 388 mm. The total fatty acid and lipid contents (mg g–1) increased with growth, and the percent composition of fatty acids and lipids changed drastically. The percentages of docosahexaenoic acid (%DHA), stearic acid (%Ste), and arachidonic acid (%ARA) decreased and the percentage of oleic acid (%Ole) increased when the body length (fork length, FL) was about 250 mm. The amounts of triacylglycerols (TAG) in fish in the East China Sea were lower than in the Sea of Japan and Pacific Ocean. The percentage of TAG drastically increased at around 250 mm FL, however, the phosphatidylcholines (PC) drastically decreased. The ontogenetic differences in the fatty acid and lipid composition might be influenced by metabolic events such as growth and reproduction. The geographical differences in chub mackerel can be distinguished by using stable isotope ratios, the ω3:ω6 fatty acid and DHA:EPA (eicosapentaenoic acid) ratios, and six main fatty acids. These chemical biomarkers could be available for understanding habitat and migration of chub mackerel.
  • Association between fatty acid signature and growth rate of larval Pacific bluefin tuna in two major spawning grounds
    Y Hiraoka; T Ishihara; A Tawa; Y Tanaka; S Ohshimo; Y Ando
    Marine Ecology Progress Series, 689, 127, 136, Inter-Research Science Center, 12 May 2022, [Peer-reviewed]
    Scientific journal, Fatty acid composition and total fatty acids of field-caught Pacific bluefin tuna (PBF) Thunnus orientalis larvae were investigated to identify relationships between spawning ground, physiological condition, and growth rate. Multivariate statistical analyses revealed that both growth rate and environmental conditions at spawning grounds were substantially associated with variations in fatty acid compositions. Fast-growing larvae typically contained more α-linolenic acid and 22:5n-3, which are important as metabolic precursors of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Larval PBF fatty acid compositions differed by spawning ground; larvae caught around the Nansei Islands contained more 15:0, 17:0, 19:0, and 22:5n-6, and less EPA and arachidonic acid (ARA) than those from the Sea of Japan. Differences in larval odd-numbered fatty acid compositions might indicate different degrees of dependence on microbial loop energy supply between spawning grounds. Environments subject to sudden changes, such as those in water temperature and prey density in the Sea of Japan, might cause variability in fatty acid profiles, including extremely low %DHA. We suggest that continuous food intake and subsequent fatty acid catabolism for energy generation would be needed to facilitate fast growth.
  • Lipid and fatty acid dynamics by maternal Pacific bluefin tuna.
    Hiraoka Y; Okochi Y; Ohshimo S; Shimose T; Ashida H; Sato T; Ando Y
    PloS one, 14, 9, e0222824, 2019, [Peer-reviewed]
  • A simplified gas chromatographic fatty acid analysis by the direct saponification/methylation procedure and its application of Pacific bluefin tuna, yellowfin tuna and skipjack tuna larvae.
    Matsumoto Y; Ando, Y; Hiraoka, Y; Tawa, A; Ohshimo, S
    Lipids, Dec. 2018, [Peer-reviewed]
    English, Scientific journal
  • A Simplified Gas Chromatographic Fatty-Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae.
    Matsumoto Y; Ando Y; Hiraoka Y; Tawa A; Ohshimo S
    Lipids, 53, 9, 919, 929, Sep. 2018, [Peer-reviewed]
  • Stereospecific Analysis of Fish Oil Triacylglycerols by Silicic Acid HPLC Using Monoacylglycerol Analytical Intermediates
    ANDO Yasuhiro; MATSUMOTO Nobuto; YONETA Yukino; TADA Naoto
    Bulletin of Fisheries Sciences, Hokkaido University, 68, 2, 33, 41, 02 Aug. 2018
    English, Research institution
  • Lipid Profiles of 28 Minor Marine Fish Species Caught from the Waters around Hokkaido, Japan
    中塚 美紀子; 安藤 靖浩
    北海道大学水産科学研究彙報, 66, 2, 69, 76, 北海道大学大学院水産科学研究院, Aug. 2016
    Japanese, Lipid content, lipid class composition, and fatty acid composition were determined for 28 minor marine fish species caught from the waters around Hokkaido, Japan. The samples include some rare fish such as dusky rockfish Sebastes variabilis "nagamenuke" and prowfish Zaprora silenus "bouzuginpo." Most samples had low lipid contents, and therefore, can be used in low-fat fish foods. In contrast, the fish of four species and the liver of eight species were rich in lipid as they contained triacylgycerols or diacylglycerylethers as major lipid classes. Such fatty fish can be used as a source of marine lipids. The liver of brown hakeling Physiculus maximowiczi "donko" and ocean sunfish Mola mola "manbou" was found to be useful for including EPA and DHA. Prowfish and other two species were shown to contain tetracosahexaenoic acid in their fatty flesh and/or liver. These findings are useful fundamental information on the minor marine fish.
  • Synthesis of Urethane Derivatives of Mono- and Diacylglycerols for Use as HPLC Standards in the Enantiomeric Separation
    Naoto Tada; Honami Fujita; Yasuhiro Ando
    JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 91, 7, 1131, 1137, Jul. 2014, [Peer-reviewed]
    English, Scientific journal
  • Individual variations in fatty acid composition and concentration as indicators of the nutritional condition of wild pointhead flounder larvae
    Yuko Hiraoka; Tetsuya Takatsu; Yasuhiro Ando
    MARINE BIOLOGY, 161, 7, 1615, 1625, Jul. 2014, [Peer-reviewed]
    English, Scientific journal
  • Occurrence and distribution of cis-7-icosenoic acid in the lipids of Japanese marine fish, shellfish, and crustaceans
    Yutarou Nakamura; Yuuki Fukuda; Kana Shimizu; Yasuhiro Ando
    FISHERIES SCIENCE, 80, 3, 613, 620, May 2014, [Peer-reviewed]
    English, Scientific journal
  • Gas Chromatographic Equivalent Chain Length (ECL) Values of Fatty Acid Methyl Esters on a Highly Polar Ionic Liquid Column, SLB-IL111
    NAKAMUA Yutarou; SHIMIZU Kana; ANDO Yasuhiro
    Bulletin of Fisheries Sciences, Hokkaido University, 64, 1, 9, 16, 北海道大学大学院水産科学研究科編集出版委員会, 14 Mar. 2014
    English, Scientific journal, This paper reports the retention data of fatty acid methyl esters on a novel ionic liquid column, SLB-IL111, which hashigher polarity than commercially available columns for gas chromatography (GC). The fatty acids analyzed using GC includebranched-chain saturated fatty acids, C16-C22 monounsaturated fatty acid cis/trans and positional isomers, and polyunsaturatedfatty acids commonly found in natural products. Fatty acids of sardine oil were also analyzed using GC. GC was carried outon a 100-m column under isothermal conditions within the range 120-170°C, using helium as a carrier gas. The retention dataof fatty acid methyl esters were represented by equivalent chain length (ECL) values calculated from the retention times of eachfatty acid related to those of saturated fatty acids with an even carbon number. This is novel information, obtained on an SLBIL111column being operated at various temperatures with a single GC system. The results show that the column temperaturegreatly affected the ECL values of unsaturated fatty acids. Thus, when using this column for the analysis of fish oil fattya cids, itis necessary to precisely control the column temperature.
  • Gas Chromatographic Separation of Docosenoic Acid Positional Isomers on an SLB-IL100 Ionic Liquid Column
    Kana Shimizu; Yasuhiro Ando
    JOURNAL OF OLEO SCIENCE, 61, 8, 421, 426, Aug. 2012, [Peer-reviewed]
    English, Scientific journal
  • Occurrence of all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3) in roughscale sole Clidoderma asperrimum flesh lipids
    Yuuki Fukuda; Yasuhiro Ando
    FISHERIES SCIENCE, 77, 5, 875, 882, Sep. 2011, [Peer-reviewed]
    English, Scientific journal
  • GC Separation of cis-Eicosenoic Acid Positional Isomers on an Ionic Liquid SLB-IL100 Stationary Phase
    Yasuhiro Ando; Tsubasa Sasaki
    JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 88, 6, 743, 748, Jun. 2011, [Peer-reviewed]
    English, Scientific journal
  • Purification of 9-Anthracenemethanol as a Fluorescent-Labeling Reagent for Microanalysis of Fatty Acids
    HABA YUSUKE; ANDO YASUHIRO
    北海道大学水産科学研究い報, 59, 3, 67, 72, 北海道大学大学院水産科学研究院 = Research Faculty of Fisheries Sciences, Hokkaido University, 08 Dec. 2009
    Japanese, A fluorescent-labeling reagent 9-anthracenemethanol was purified by recrystallization for use in microanalysis of natural oil fatty acids. A commercial reagent was dissolved in ethyl acetate at the boiling point, and allowed to stand at room temperature until the crystals precipitated in yellow needles. The product given in the yield of 74% was analyzed by reversed-phase high-performance liquid chromatography. Many contaminant peaks found in the crude material disappeared in the chromatogram, indicating successful purification of the reagent. The purified reagent could be used for fatty acid analysis of 1-5μg of natural triacylglycerols including those of cod liver/mackerel oil and bonito head oil. 2-Butanone was also effective solvent for the purification. With these solvents, commercially available 9-anthracenemethanol can be purified by the simple recrystallization.
  • Reinvestigation of positional distribution of tetracosahexaenoic acid in triacyl-sn-glycerols of flathead flounder flesh
    Yuki Tomita; Yasuhiro Ando
    FISHERIES SCIENCE, 75, 2, 445, 451, Apr. 2009, [Peer-reviewed]
    English, Scientific journal
  • The variation of density and sound speed contrasts and theoretical target strength estimation of Euphausia pacifica
    Ryuichi Matsukura; Tohru Mukai; Yasuhiro Ando; Kohji Iida
    NIPPON SUISAN GAKKAISHI, 75, 1, 38, 44, Jan. 2009, [Peer-reviewed]
    Japanese, Scientific journal
  • Preparation of Ethyl Magnesium Bromide for Regiospecific Analysis of Triacylglycerols
    Yasuhiro Ando; Yuki Tomita; Yusuke Haba
    JOURNAL OF OLEO SCIENCE, 57, 8, 459, 462, Aug. 2008, [Peer-reviewed]
    English, Scientific journal
  • Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography
    ANDO YASUHIRO; HABA YUSUKE; TAKASE KIWAMU; SAKAI SHINYA
    北海道大学水産科学研究い報, 57, 3, 37, 41, 北海道大学大学院水産科学研究院 = RESEARCH FACULTY OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY, 13 Dec. 2007
    English, This paper presents a smaller-sized procedure for regiospecific analysis of triacylglycerols (TAG) using boric acid high-performance thin-layer chromatography (HPTLC). Cod liver/mackerel, bonito head, and seal oils TAG (2mg) were partially hydrolyzed by ethyl magnesium bromide, and resulting 1(3)-and 2-monoacylglycerols (MAG) were isolated by the HPTLC. Fatty acids of the 1(3)-and 2-MAG were analyzed by gas-liquid chromatography (GLC). Positional distributions of fatty acids in TAG observed for the three marine oils were similar to those previously determined by the procedure requiring 100mg of starting TAG. The new procedure appeared to be usable for regiospecific analysis of a few milligrams of marine oils TAG.
  • Rapid preparation of fluorescent 9-anthrylmethyl esters for fatty acid analysis of small amount of triacylglycerols
    Yasuhiro Ando; Yusuke Haba; Kazutaka Soma; Yuko Hiraoka; Tetsuya Takatsu
    LIPIDS, 42, 10, 955, 960, Oct. 2007, [Peer-reviewed]
    English, Scientific journal
  • Lipid Profile of Mysid Neomysis intermedia and Its Changes by Feeding DHA-Rich Marine Lipids
    ANDO YASUHIRO; NOZAKI MANTARO
    J Oleo Sci, 56, 9, 471-478 (J-STAGE), 478, Japan Oil Chemists' Society, 2007, [Peer-reviewed]
    English, There has been no information on lipids of brackish water mysid Neomysis intermedia, which is the most important mysid for human food in Japan. The present study revealed their lipid content, lipid class composition, and fatty acid compositions of total lipids (TL) and major lipid classes. Lipid content of the mysid was 1.0% on wet-weight base. Major lipid classes were phosphatidylcholines (PC, 26%), phosphatidylethanolamines (PE, 24%), and phosphatidylinositols (PI, 14%), whereas the content of triacylglycerols (TAG) was relatively low (7%). Major fatty acids of the TL were 16:0 (21%), 18:1n-9 (12%), 20:5n-3 (EPA; 19%) and 22:6n-3 (DHA; 14%), and the latter two polyunsaturated fatty acids were concentrated in PI (totally 47%) and PE (43%) fractions rather than in PC (20%) and TAG (18%). Arachidonic acid (4%) was also higher in the PI (7%) and PE (5%). When the mysid was fed tri-, di- and monoacylglycerols prepared from DHA-rich fish oil, DHA mainly increased in the TAG fraction of the mysid. The mysid appeared to primarily incorporate exogenous DHA into TAG fraction.
  • Fatty Acid Enrichment of Torifers Using Type-A Gelatin Solution
    Ando Yasuhiro; Abe Tomomi; Ookubo Yuka; Namikawa Saori
    Bulletin of Fisheries Sciences, Hokkaido University, 56, 3, 61, 65, Hokkaido University, 22 Dec. 2005
    English
  • Positional Distribution of DHA in Triacyl-sn-glycerols of Artemia Nauplii Enriched with Salmon Roe Polar Lipids-Containing Fish Oil
    SHIOZAKI Rieko; ANDO Yasuhiro
    Journal of Oleo Science, 54, 3, 161, 166, Japan Oil Chemists' Society, 2005, [Peer-reviewed]
    English, Positional distribution of fatty acids in triacyl-sn-glycerols (TAG) has been investigated for Artemia franciscana nauplii used in marine fish larviculture as an important live food. For enhancement of essential highly unsaturated fatty acids, the nauplii were enriched for 4 to 24 h with two emulsions containing fish roe polar lipids (PL), (i) a mixture of bonito head oil TAG and salmon roe PL concentrate (88:12, wt/wt) and (ii) an unmixed salmon roe PL concentrate. In TAG isolated from all enriched Artemia nauplii, docosahexaenoic acid (DHA) was preferentially esterified in the sn-3 position (0.7-22.9 mol%) followed in sequence by the sn-2 (0.1-7.5 mol%) and sn-1 (0.0-4.6 mol%) positions. This distribution pattern was the same as previously observed for enrichment with bonito head oil TAG free from salmon roe PL. Addition of fish roe PL to DHA-rich fish oil enricher does not seem to practically affect the distribution pattern of DHA characteristic of Artemia nauplii.
  • Positional distribution of docosahexaenoic acid in triacyl-sn-glycerols of rotifers Brachionus plicatilis enriched with fish oil fatty acid ethyl esters
    Y Ando; S Kobayashi
    AQUACULTURE RESEARCH, 35, 14, 1391, 1394, Nov. 2004, [Peer-reviewed]
    English, Scientific journal
  • Positional distribution of DHA and EPA in triacyl-sn-glycerols (TAG) of Artemia franciscana nauplii enriched with fish oils ethyl esters and TAG
    Y Ando; H Samoto; Y Murayama
    AQUACULTURE, 233, 1-4, 321, 335, Apr. 2004, [Peer-reviewed]
    English, Scientific journal
  • Lipid Profile of Krill Euphausia pacifica Collected in the Pacific Ocean near Funka Bay, Hokkaido, Japan
    Nami Kusumoto; Yasuhiro Ando; Ryuichi Matsukura; Tohru Mukai
    Journal of Oleo Science, 53, 1, 45, 51, 2004, [Peer-reviewed]
    English, Scientific journal
  • Positional distribution of n-3 highly unsaturated fatty acids in triacyl-sn-glycerols (TAG) of rotifers (Brachionus plicatilis) enriched with fish and seal oils TAG
    Y Ando; S Kobayashi; T Sugimoto; N Takamaru
    AQUACULTURE, 229, 1-4, 275, 288, Jan. 2004, [Peer-reviewed]
    English, Scientific journal
  • Lipid Content and Fatty Acid Composition of Euphausia pacifica(Total Body Lengths Less than 13mm) Collected in the Water near Funka Bay.
    Kusumoto Nami; Ando Yasuhiro; Matsukura Ryuichi; Mukai Tohru
    Bulletin of Fisheries Sciences, Hokkaido University, 54, 3, 73, 77, Hokkaido University, 2003
    English
  • Preparation of Salmon Roe Polar Lipid Concentrate as an HUFA Enricher of Artemia Nauplii
    Yasuhiro Ando; Hiromi Samoto; Shigefumi Sasaki
    Journal of Oleo Science, 52, 8, 443, 447, 2003, [Peer-reviewed]
    English, Scientific journal
  • Regiospecific distribution of fatty acids in triacylglycerols of Artemia franciscana nauplii enriched with fatty acid ethyl esters
    Y Ando; Y Oomi; K Narukawa
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 133, 2, 191, 199, Oct. 2002, [Peer-reviewed]
    English, Scientific journal
  • Fatty acid composition of two species of Japanese freshwater sponges Heterorotula multidentata and Spongilla alba
    NU Sata; M Kaneniwa; Y Masuda; Y Ando; H IIda
    FISHERIES SCIENCE, 68, 1, 236, 238, Feb. 2002, [Peer-reviewed]
    English, Scientific journal
  • Regiospecific Distribution of Highly Unsaturated Fatty Acids in Triacylglycerols of Artemia Nauplii Enriched with Marine Oils
    Yasuhiro Ando; Keiichi Narukawa
    Journal of Oleo Science, 51, 9, 615, 620, 2002, [Peer-reviewed]
    English, Scientific journal
  • Distribution of Novel Nonmethylene-Interrupted Fatty Acids over Neutral and Polar Lipids of Ophiuroidea (Brittle Star)
    Daisuke Sato; Yasuhiro Ando
    Journal of Oleo Science, 51, 8, 563, 567, Japan Oil Chemists' Society, 2002, [Peer-reviewed]
    English, Scientific journal
  • Identification of novel nonmethylene-interrupted fatty acids, 7E,13E-20 : 2, 7E,13E,17Z-20 : 3, 9E,15E,19Z-22 : 3, and 4Z,9E,15E,19Z-22 : 4, in Ophiuroidea (brittle star) lipids
    D Sato; Y Ando; R Tsujimoto; K Kawasaki
    LIPIDS, 36, 12, 1371, 1375, Dec. 2001, [Peer-reviewed]
    English, Scientific journal
  • Positional distribution of highly unsaturated fatty acids in triacyl-sn-glycerols of Artemia nauplii enriched with docosahexaenoic acid ethyl ester
    Y Ando; Y Oomi
    LIPIDS, 36, 7, 733, 740, Jul. 2001, [Peer-reviewed]
    English, Scientific journal
  • Reinvestigation of positional distribution of fatty acids in docosahexaenoic acid-rich fish oil triacyl-sn-glycerols
    Y Ando; M Satake; Y Takahashi
    LIPIDS, 35, 5, 579, 582, May 2000, [Peer-reviewed]
    English, Scientific journal
  • Identification of 5-octadecenoic acid in the rotifer Brachionus plicatilis
    Y Ando; S Morita; N Tadokoro; T Ota; T Sugimoto; N Takamaru
    FISHERIES SCIENCE, 65, 5, 808, 809, Oct. 1999, [Peer-reviewed]
    English, Scientific journal
  • Deleterious effect of diatom diets on egg production and hatching success in the marine copepod Pseudocalanus newmani
    LEE Hong - Wu; BAN Syuhei; ANDO Yasuhiro; OTA Toru; IKEDA Tsutomu
    Plankton Biology and Ecology, 46, 2, 104, 112, Plankton Society of Japan, 01 Aug. 1999, [Peer-reviewed]
    English
  • Stereospecific Analysis of Triacyl-sn-glycerols by HPLC
    ANDO Yasuhiro
    Journal of Japan Oil Chemists' Society, 47, 10, 953, 962, Japan Oil Chemists' Society, 20 Oct. 1998, [Peer-reviewed]
    Japanese, High-performance liquid chromatography (HPLC) for stereospecific analysis of triacyl-<I>sn</I>-glycerols (TG) does not have the drawbacks attending enzymatic methods and involves the separation of isomeric and enantiomeric partial acylglycerols, <I>i.e.</I>, monoacylglycerols (MG) and diacylglycerols (DG), farmed from TG. In this method, the Grignard hydrolysis of TG to partial acylglycerols and their isolation are carried out followed by the preparation of enantiomeric or diastereomeric urethane derivatives, their resolution by chiral- or achiral-phase HPLC and fatty acid analysis as methyl esters. The accuracy of this method depends on acyl group migration during analysis, differential loss in conversion, resolution of enantiomers and calculation for acyl compositions in <I>sn</I>-1, <I>sn</I>-2, and <I>sn</I>-3 positions. Soybean, pine seed, yew seed, and saucy oil TG have been analyzed.
  • Demospongic acids of the marine sponge Halichondria panicea from the coast of Hokkaido, Japan
    Y Ando; Y Kawabata; K Narukawa; T Ota
    FISHERIES SCIENCE, 64, 1, 136, 139, Feb. 1998, [Peer-reviewed]
    English, Scientific journal
  • Phagostimulant activity of phosphatidylcholine molecular species for young abalone Haliotis discus hannai
    Y Ando; J Nakamura; T Ota
    FISHERIES SCIENCE, 63, 6, 1048, 1049, Dec. 1997, [Peer-reviewed]
    English, Scientific journal
  • Lipids and fatty acids in Artemia nauplii enriched with fish oil triacylglycerols containing docosahexaenoic acid in different positional distribution patterns
    Y Ando; M Kotake; T Ota
    FISHERIES SCIENCE, 63, 4, 605, 609, Aug. 1997, [Peer-reviewed]
    English, Scientific journal
  • Stereospecific analysis of triacyl-sn-glycerols in docosahexaenoic acid-rich fish oils
    Y Ando; T Ota; Y Matsuhira; K Yazawa
    JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 73, 4, 483, 487, Apr. 1996, [Peer-reviewed]
    English, Scientific journal
  • Changes in positional distribution of fatty acids in dorsal muscle triacyl-sn-glycerols from chum salmon, Oncorhynchus keta, at spawning season
    Y Ando; A Shimoyama; T Ota
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 113, 1, 89, 93, Jan. 1996, [Peer-reviewed]
    English, Scientific journal
  • Steric Structures of Triacylglycerols in the Seed Oil of Carrot Daucus carota L. var. sativa DC.
    TAKAGI Toru; ANDO Yasuhiro; ICHIKAWA Kazuaki
    Journal of Japan Oil Chemists' Society, 44, 11, 971, 976, Japan Oil Chemists' Society, 20 Nov. 1995, [Peer-reviewed]
    English, The steric structures of the triacylglycerols (TGs) in the seed oil of <I>carrot Daucus carota</I> L. var. <I>sativa</I> DC. were studied by the stereospecific analysis of the acyl distributions using the chiral-phase high-performance liquid chromatography of the monoacylglycerol derivatives. Total fatty acids of the seed oil TGs contained 71 mol % petroselinic acid (6-18 : 1), 12 mol % oleic acid (9-18 : 1), 11 mol %linoleic acid (18 : 2 <I>n</I>-6), and 4 mol % palmitic acid (16 : 0). Fatty acid percents in each <I>sn</I>-1, <I>sn</I>-2 and <I>sn</I>-3 position of the TGs were 6-18 : 1 (38, 23 and 40 %), 9-18 : 1 (21, 66 and 13 %), and 18 : 2 <I>n</I>-6 (20, 65and 15 %). Structures and estimated contents of the major TG components in the carrot seed oil wasfound to be (6-18 : 1) (6-18 : 1) (6-18 : 1) 3248 %, (6-18 : 1) (9-18 : 1) (6-18 : 1) 1725 %, (6-18 : 1) (18 : 2 <I>n</I>-6) (6-18 : 1) 1522 %. Petroselinic acid distributes preferentially at the <I>sn</I>-1 and <I>sn</I>-3 positionsin the carrot seed oil TGs in analogy with 16 : 0 and 18 : 0 in the ordinary seed oil TGs.
  • トリアシルグリセロールの立体特異分析
    安藤 靖浩; 板橋 豊
    化学と生物, 33, 8, 545, 552, 学会出版センタ-, 25 Aug. 1995, [Peer-reviewed]
    Japanese
  • POSITIONAL DISTRIBUTION OF 24/6(N-3) IN TRIACYL-SN-GLYCEROLS FROM FLATHEAD FLOUNDER LIVER AND FLESH
    T OTA; Y KAWABATA; Y ANDO
    JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 71, 5, 475, 478, May 1994, [Peer-reviewed]
    English, Scientific journal
  • Stereospecific Analysis of Triacylglycerols by Chiral-Phase HPLC Direct Derivation of Partially Hydrolyzed Products
    TAKAGI Toru; ANDO Yasuhiro
    Journal of Japan Oil Chemists' Society, 40, 4, 288, 292, Japan Oil Chemists' Society, 1991, [Peer-reviewed]
    A modified method for the stereospecific analysis of triacylglycerols (TGs) is presented. Partially hydrolyzed products of TG with ethyl magnesium bromide were converted to 3, 5-dinitrophenylurethane (3, 5-DNPU) derivatives, and recemic 1-and 2-monoacylglycerol (MG) derivatives were separated by thin layer chromatography (TLC) on silicic acid plates. The 1-MG fraction was separated into <I>sn</I>-1 and <I>sn</I>-3 enantiomers by high performance liquid chromatography (HPLC) on a chiral stationary phase; <I>N</I>- (<I>R</I>) -1- (α-naphthyl) ethylaminocarbonyl- (<I>S</I>) -valine which was chemically bound to γ-aminopropyl silanized silica. Total TG, <I>sn</I>-1, <I>sn</I>-2 and sn-3 MG fractions were converted to the methyl esters, and each of which was analyzed by gas chromatography (GC) with an opentubular column. Acyl distribution at <I>sn</I>-1, <I>sn</I>-2 and <I>sn</I>-3 positions was determined based on the results of GC analysis. A cacao fat sample was analyzed by this method. Direct derivation prior to fractionation was found effective for reducing isomerization of MGs.
  • Stereospecific Analysis of Acyl Group Distribution in Triacylglycerols by HPLC with Chiral Column
    TAKAGI Toru; ANDO Yasuhiro
    Journal of Japan Oil Chemists' Society, 39, 9, 622, 628, Japan Oil Chemists' Society, 1990, [Peer-reviewed]
    High-performance liquid chromatography (HPLC) using a chiral stationary phase <I>N</I>- (S) -2- (4-chlorophenyl) -D-isovaleroyl-D-phenylglycine chemically bound to γ-aminopropyl silanized silica (Sumipax OA 2100) was improved for the separation of homologous mixtures of saturated and unsaturated 1-monoacylglycerol (MG) enantiomers as 3, 5-dinitrophenylurethane (3, 5-DNPU) derivatives. By this HPLC method, it was possible to separate three saturated 1-MGs having consecutive even-carbon numbers, and 1-MG mixtures of 18 : 0, 18 : 1, 18 : 2, and 18 : 3 to <I>sn</I>-1 and <I>sn</I>-3 enantiomers of each component. It was used for the stereospecific analysis of acyl group distributions in triacylglycerols from linseed oil.<BR>A 1-MG sample from the degradation of TGs with Grignard reagent was fractionated to saturated and unsaturated fractions by thin layer chromatography on silver nitrate and boric acid-impregnated silicic acid plates. The total, saturated and unsaturated 1-MG samples were then analyzed as 3, 5-DNPU derivatives by HPLC on the chiral stationary phase. Acyl group distributions at the <I>sn</I>-1, <I>sn</I>-2 and <I>sn</I>-3 positions were determined based on the data obtained.
  • JAPANESE SARDINE OIL AS A SOURCE OF 16-3(N-4) AND 16-4(N-1) FATTY-ACIDS
    Y ANDO; T OTA; T TAKAGI
    JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 66, 9, 1323, 1325, Sep. 1989, [Peer-reviewed]
    English, Scientific journal
■ Other Activities and Achievements
■ Books and other publications
■ Lectures, oral presentations, etc.
■ Syllabus
  • 生物資源化学特論Ⅱ, 2024年, 修士課程, 水産科学院
  • 有機化学, 2024年, 学士課程, 水産学部
  • 健康と社会, 2024年, 学士課程, 全学教育
  • 環境と人間, 2024年, 学士課程, 全学教育
  • 生産プロセス工学実習, 2024年, 学士課程, 水産学部
  • 水産科学英語Ⅱ, 2024年, 学士課程, 水産学部
■ Affiliated academic society
  • 日本水産学会
  • アメリカ油化学会
  • 日本油化学会
  • The American Oil Chemists' Society
  • Japan Oil Chemists' Society
  • The Japanese Society of Fisheries Science
■ Works
■ Research Themes
  • 海洋性魚類の食物連鎖解明に資するエイコセン酸異性体組成のデータベース構築
    科学研究費助成事業 基盤研究(C)
    01 Apr. 2020 - 31 Mar. 2025
    安藤 靖浩
    海産魚の食性解明に資するエイコセン酸異性体組成のデータベース構築を目的として、表層および中層の中間捕食者に焦点を当てて日本近海で採集されたマサバ、サンマ、イカ3種、ハダカイワシ9種の合計105検体のエイコセン酸の6種の異性体（20:1n-15, n-13, n-11, n-9, n-7, n-5）の組成を決定した。
    マサバとサンマの筋肉では20:1n-11と20:1n-9が主要異性体であった。これら2つの異性体の組成値は海域による差異が顕著であった。北西太平洋、三陸沖、福島沖では20:1n-11が全20:1の60％以上を占め最も割合が高かったのに対し、東シナ海では20:1n-9が60-80％と首位が逆転した。イカは外套膜では20:1n-9が90％以上であり海域間の差異は認められなかった。肝臓においても外套膜と同様に20:1n-9が最も高い割合であったが、それに次ぐ20:1n-11は三陸沖と石川沖で20-30％であったのに対し東シナ海は10％に満たず、海域間の違いが認められた。ハダカイワシの筋肉は、福島沖と東シナ海の4種で20:1n-9が60％以上で最も多かった。一方、三陸沖の5種は20:1n-9が多い2種と20:1n-11が多い3種に分かれ種間の違いが認められた。三陸沖のハダカイワシは種ごとに鉛直移動を行う水深が異なることが知られており、本研究の結果は深い生息域をもつ種で20:1n-11が優先することを示した。
    以上、表層魚と中層魚は同一種あるいは近縁種であっても海域や水深によってエイコセン酸異性体組成が異なることを明らかにすることができた。異性体組成の海域差は太平洋と大西洋といった大洋間だけでなく、日本周辺でも認められることが初めて明らかとなった。


    日本学術振興会, 基盤研究(C), 北海道大学, 20K06199
  • 脂肪酸を用いたクロマグロ仔魚の高成長維持機構の解明
    科学研究費助成事業 基盤研究(C)
    01 Apr. 2021 - 31 Mar. 2024
    平岡 優子; 安藤 靖浩; 橋本 博
    日本学術振興会, 基盤研究(C), 国立研究開発法人水産研究・教育機構, 21K05738
  • 脂質・脂肪酸分析を用いたクロマグロの繁殖投資量の推定と産卵能力の評価
    科学研究費助成事業 基盤研究(C)
    01 Apr. 2020 - 31 Mar. 2023
    芦田 拡士; 安藤 靖浩
    クロマグロは1産卵期に1個体が複数回産卵する多回性産卵魚である。多回性産卵魚では1回あたりの産卵数は個体の内的・外的要因によって変動することが知られている。本課題では、産卵数と卵1個当たりの脂質・脂肪酸量を用いて、1回の産卵で卵に投資する脂質・脂肪酸量 (繁殖投資量)を推定することを目的とする。
    今年度のクロマグロの卵巣サンプリングを通じ、50個体の吸水卵を持つ卵巣を収集することができた。また、脂質・脂肪酸分析の実施予定のある38個体において1回あたりの産卵数を推定した。南西諸島では1回あたりの産卵数は尾叉長、漁期開始からの経過日数が有意な影響を与えることが明らかになった。
    昨年度に開発した脂質・脂肪酸用分析用の吸水卵のサンプリング方法に不備が発見され、一部の個体の脂質・脂肪酸分析値にバイアスが含まれる可能性が明らかになった。そのため、サンプリング方法を改善し、吸水卵の脂質・脂肪酸の再分析を行った。吸水卵の1個当たりの総脂肪酸量は卵1個当たりの乾燥重量と正の相関がある事が改めて確認した。一方で、卵1個当たりの総脂肪酸量は1回あたりの産卵数、表面水温、尾叉長、肥満度とは有意な相関は認められなかった。
    1個の吸水卵に含まれる脂質量を推定するために、13個体から卵1個当たりの脂質および脂肪酸量をそれぞれ測定し、卵1個における総脂肪酸量-総脂質量の回帰式を作成した。作成した回帰式、個体の卵1個当たりの総脂肪酸量および1回あたりの産卵数を用いて、1回あたりの産卵で吸水卵に投資される総脂質量(繁殖投資量)を32個体から推定した。推定された繁殖投資量は個体の体サイズと正の相関が認められた。
    日本学術振興会, 基盤研究(C), 国立研究開発法人水産研究・教育機構, 20K06197
  • Analytical Method for Nutirtional Condition of Marine Fish Larvae by Individual
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    2003 - 2004
    ANDO Yasuhiro; TAKATSU Tetsuya
    A sensitive and convenient procedure for fatty acid analysis of marine fish larvae has been developed in order to determine docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) contents as an indicator of their nutritional condition. Fatty acid content and composition in each individual of the wild marine fish larvae have been analyzed by the new procedure.1.High-performance liquid chromatography (HPLC) of fatty acid 9-anthrylmethyl esters was introduced to the new procedure. Highly sensitive fluorescent detection of the esters enabled the analysis of one eye and somatic part from one individual.2.Some processes of the analysis were modified to reduce time-consuming complicated operations. All processes after lipid extraction could be carried out in a single disposable glass vial. The analysis was completed within 3.5 hours, and about ten samples could be analyzed in one day.3.Somatic part of the 326 individuals of wild fish larvae (18 species) caught in the Funka and Mutsu Bays, Japan, were subjected to the fatty acid analysis. In all individuals, remarkable fatty acids were only five components, DHA (18-32 %), palmitic acid (18-27 %), EPA (4-19 %), octadecenoic acid (7-12 %) and stearic acid (4-23 %).4.DHA contents of the somatic part (μg/individual) were summarized for every nine individuals classified by body lengths. Coefficient of variance (CV) was high in the 2.4-3.1 and 4.4-5.2 mm brown sole (CV 0.6-1.0) and 4.8-6.0 mm Japanese anchovy (CV 0.7-0.9). Pacific sandlance showed generally lower CV (0.2-0.5 with one exception 0.9). The contents of DHA essential for marine fish larvae widely vary at specific stages during development of wild marine fish larvae.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), HOKKAIDO UNIVERSITY, Principal investigator, Competitive research funding, 15580153
  • Analysis, distribution and function of unnatural glycerophospholipids in marine organisms
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    2001 - 2003
    ITABASHI Yutaka; ANDO Yasuhiro; SAWABE Tomoo
    The aim of this study was to clarify the distribution and physiological function of unusual glycerophospho- and glycolipids in marine organisms. For this purpose, chiral phase HPLC, reversed-phase HPLC and reversed-phase HPLC in conjunction with electrospray ionization mass spectrometry(ESI-MS) were applied to the separation and identification of stereoisomeric and regio-isomeric glycerolipids. Using these techniques, we found an unnatural phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-3'-sn-glycerol, R, R configurations at the two chiral centers) from various kinds of microorganisms including marine bacteria. The R, R isomer content increased with increasing culture temperature. The results strongly suggest that the bacteria adapt to temperature by alternating not only the degree of unsaturation but also the stereoisomer composition of phosphatidylglycerols. In the present study, we also investigated the separation and identification of the reverse isomers of glycerophospholipids (PtdCho,PtdEtn,PtdIno) and glycoglycerolipids (MGDG,DGDG,SQDG) by reversed-phase HPLC/ESI-MS. These glycerolipids were chromatographed as intact forms or 3,5-dinitrophenylurethanes of the 1,2-diacyl-sn-glycerols derived from the parent molecules. Excellent resolution was achieved for reverse isomers of very different pairs of acyl groups, such as 20:5-16:0 and 22:6-16:0, on highly efficient C18 columns. Using the method, we analyzed the glycerolipids from some fish and marine algae, and found that only MGDG from marine red algae, such as Porphyra yezoensis and Gracilaria verrucosa, gave two clearly resolved peaks, representing the reverse isomers of 20:5(sn-1)/16:0(sn-2) and 16:0(sn-1)/20:5(sn-2), and 20:4(sn-1)/16:0(sn-2) and 16:0(sn-1)/20:5(sn-2), respectively. The present method provides the first direct demonstration of the occurrence of reverse isomers in natural glycoglycerolipids and should be helpful in promoting work on the biosynthetic pathway and physiological importance of the reverse isomers in the red algae.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 13460088
  • 脂溶性摂餌刺激物質の分子種組成による水産餌料評価に関する研究
    科学研究費助成事業 奨励研究(A)
    1995 - 1995
    安藤 靖浩
    1.魚介類の種苗生産で利用される餌料用植物プランクトンについて、脂溶性摂餌刺激物質であるホスファチジルコリン、ジガラクトシルジアシルグリセロール、スルホキノボシルジアシルグリセロールを含む脂質成分の分析を行った。(1)脂肪酸組成を決定した。使用した5種の植物プランクトンでは構成脂肪酸が大きく異なり、飽和酸およびモノエン酸が主要成分であるもの、ジエンないしテトラエン酸を多く含むもの、ペンタエン以上の不飽和酸を著量含むものが存在することを明らかにした。(2)P.lutheriの脂質から摂餌刺激物質を単離し、含有率と構成脂肪酸を決定した。上記の摂餌刺激物質は脂肪酸含有脂質の約3分の1を占めた。ホスファチジルコリンとジガラクトシルジアシルグリセロールには高度不飽和酸が40〜60%含まれ、これらの摂餌刺激物質では不飽和度の高い分子種が多量に存在する可能性を明らかにした。2.天然の脂溶性摂餌刺激物質を構成し得る分子種を使用して、水産生物に対する摂餌刺激活性試験を実施した。(1)藻食性巻貝に対する活性試験として結晶セルロース板(アビセル板)法の適用を検討した。試験板の解析方法をわずかに改変することによって、摂餌刺激物質の分子種の場合でも本法が活性の検出・判定に有効であることを確認した。(2)植物脂質含有性で構造既知のホスファチジルコリン分子種を選定し、エゾアワビに対する摂餌刺激活性を比較した。分子種の活性は一様ではなく、特定の分子種間の活性には大きな有意差が存在した。活性と分子種の構造(不飽和度・炭素鎖長・異性体など)との関連性を検討し、供試試料(13分子種)の範囲内では分子種の不飽和度の違いがホスファチジルコリンの摂餌刺激活性に関与することを明らかにした。
    日本学術振興会, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 07760197
  • 水産脂質成分の組成および構造分析
    1987
    Competitive research funding
  • Analysis of marine lipids
    1987
    Competitive research funding