Master of Fisheries, Hokkaido University

Doctor of Fisheries, Hokkaido University

アルギン酸分解酵素

アワビ

消化管内細菌

ゲノム

Vibrio halioticoli

Pseudoalteromonas elyakovii

微生物

マコンブプロトプラスト再生

バイオ燃料

アワビ消化管内細菌群

Vibrio

細胞壁再生

エタノール

プロトプラスト

バイオマス

海水依存型発現

分子系統解析

水産学

海産食藻動物

再生可能エネルギー

微生物学

Microbiology

Life Science, Aquatic bioproduction science

Environmental Science/Agriculture Science, Environmental policy and social systems

Environmental Science/Agriculture Science, Environmental impact assessment

Life Science, Aquatic life science

Bachelor's degree program, School of Fisheries Sciences

Master's degree program, Graduate School of Fisheries Sciences

Doctoral (PhD) degree program, Graduate School of Fisheries Sciences

Apr. 2000, 日本水産学会, 日本水産学会奨励賞
Japan

Structural determination of the carotenoid glycoside ester produced by the marine bacterium Exiguobacterium sp. strain oki7 and its antioxidant and anti-inflammatory effects
Naoki Takatani; Takashi Maoka; Misaki Aoike; Munekimi Nanao; Tomoo Sawabe; Fumiaki Beppu; Masashi Hosokawa
Fisheries Science, 91, 5, 999, 1011, Springer Science and Business Media LLC, 01 Jul. 2025
Scientific journal

Genomic Repertoire of Twenty-Two Novel Vibrionaceae Species Isolated from Marine Sediments.
Hannah Kaufmann; Carolina Salvador; Vinicius W Salazar; Natália Cruz; Graciela Maria Dias; Diogo Tschoeke; Lucia Campos; Tomoo Sawabe; Masayuki Miyazaki; Fumito Maruyama; Fabiano Thompson; Cristiane Thompson
Microbial ecology, 88, 1, 36, 36, 29 Apr. 2025, [International Magazine]
English, Scientific journal, The genomic repertoire of vibrios has been extensively studied, particularly regarding their metabolic plasticity, symbiotic interactions, and resistance mechanisms to environmental stressors. However, little is known about the genomic diversity and adaptations of vibrios inhabiting deep-sea marine sediments. In this study, we investigated the genomic diversity of vibrios isolated from deep-sea core sediments collected using a manned submersible off Japan. A total of 50 vibrio isolates were obtained and characterized phenotypically, and by genome sequencing. From this total, we disclosed 22 novel species examining genome-to-genome distance, average amino acid identity, and phenotypes (Alivibrio: 1; Enterovibrio: 1; Photobacterium: 8; Vibrio: 12). The novel species have fallen within known clades (e.g., Fisheri, Enterovibrio, Profundum, and Splendidus) and novel clades (JAMM0721, JAMM0388, JAMM0395). The 28 remainder isolates were identified as known species: Aliivibrio sifiae (2), A. salmonicida (1), Enterovibrio baiacu (1), E. norvegicus (1), Photobacterium profundum (3), P. angustum (1), P. chitiniliticum (1), P. frigidiphilum (1), Photobacterium indicum (1), P. sanguinicancri (1). P. swingsii (2), Vibrio alginolyticus (3), V. anguillarum (1), V. campbellii (1), V. fluvialis (1), V. gigantis (1), V. lentus (1), V. splendidus (4), and V. tasmaniensis (1). Genomic analyses revealed that all 50 vibrios harbored genes associated with high-pressure adaptation, including sensor kinases, chaperones, autoinducer-2 (AI-2) signaling, oxidative damage repair, polyunsaturated fatty acid biosynthesis, and stress response mechanisms related to periplasmic and outer membrane protein misfolding under heat shock and osmotic stress. Additionally, alternative sigma factors, trimethylamine oxide (TMAO) respiration, and osmoprotectant acquisition pathways were identified, further supporting their ability to thrive in deep-sea environments. Notably, the genomes exhibited a high prevalence of antibiotic resistance genes, with antibiotic efflux pumps being the most abundant group. The ugd gene expanded in number in some novel species (Photobacterium satsumensis sp. nov. JAMM1754: 4 copies; Vibrio makurazakiensis sp. nov. JAMM1826: 3 copies). This gene may confer antibiotic (polymyxin) resistance to these vibrios.

Unexpected Diversity in Gene Clusters Encoding Formate Hydrogenlyase Complex Machinery in Vibrionaceae Correlated to Fermentative Hydrogen Production.
Tomoo Sawabe; Yuito Umeki; Ramesh Kumar Natarajan; Chunqi Jiang; Fabiano Thompson; Sayaka Mino
Current microbiology, 82, 5, 208, 208, 25 Mar. 2025, [International Magazine]
English, Scientific journal, An entire Hyf-type formate hydrogenlyase comple (Hyf-FHL) gene cluster was first discovered in a marine Vibrio species, Vibrio tritonius isolated from the digestive tract of the sea hare Aplysia kurodai [1]. The bacterium is also the first marine bacterium in which hydrogen production ability exceeds that of Escherichia coli under saline conditions [Sawabe et al. in Front Microbiol 4:414, 2013;Matsumura et al. in Int J Hydrog Energy 39:7270-7277, 2014;]. However, we were still unable to answer the evolutionary question as to why only minor groups of vibrios could maintain the FHL gene clusters and hydrogen (gas) production ability. Here, we set up comparative genomics and fermentative hydrogen production profiling using all 16 currently known Vibrionaceae species, which maintain FHL gene clusters and/or gas production, including 12 Vibrio and 4 Photobacterium species. Whole-genome comparison using complete genome sequences revealed unexpected diversity of FHL gene clusters, at least, with two new types of FHL gene clusters. Additional fermentative hydrogen profiling and structure modeling of FHLs showed formate detoxification as a part of formate and pH homeostasis could be one of the selective pressures in the evolution of FHL gene clusters responsible for high hydrogen production in vibrios.

Identification of flexixanthin and its derivatives in Algoriphagus bacteria and evaluation of their antioxidant and anti-inflammatory effects
Naoki Takatani; Yuki Sato-Takabe; Misaki Aoike; Rika Sekine; Takashi Maoka; Tomoo Sawabe; Fumiaki Beppu; Kazutoshi Shindo; Masashi Hosokawa
Food Bioscience, 65, Mar. 2025
Scientific journal

Deciphering Probiotic Effects of Sulfitobacter pontiacus Strain BL28 on the Host Sea Cucumber Apostichopus japonicus.
Tomoo Sawabe; Rika Kudo; Juanwen Yu; Thach Nguyen; Andrea Rossi; Yuichi Sakai; Sayaka Mino
Current microbiology, 82, 4, 156, 156, 26 Feb. 2025, [International Magazine]
English, Scientific journal, Recently, sea cucumber has been an increasingly important marine invertebrate in the fields of EcoDevo and fisheries studies. After the discovery of Sulfitobacter pontiacus strain BL28, which is a growth-promoting probiotic bacteria isolated from blastula of Apostichopus japonicus, host-microbes interaction has become a major new focus of study to be unveiled. Previous genome assessments of the strain BL28 hypothesized involvements of cobalamin/B12 biosynthesis, type IV secretion system, polyhydroxybutyrate production, and sulfate metabolism for host growth promotions. Here, we performed A. japonicus host transcriptome after five weeks of supplementation with S. pontiacus BL28. Unexpectedly, the host transcriptome revealed significant gene regulations of the BL28 supplementation on the host lipid and chondroitin metabolisms and transcriptionally "rest" status of heat shock protein genes un-expressed. Physiological and environmental crossover by the S. pontiacus BL28 supplementation may trigger stress-free growth promotion in the host sea cucumber. These symbiotics-type probiotics could help herald the future eco-friendly aquaculture of sea cucumber.

Identification of a novel monocyclic carotenoid and prediction of its biosynthetic genes in Algoriphagus sp. oki45.
Naoki Takatani; Takashi Maoka; Tomoo Sawabe; Fumiaki Beppu; Masashi Hosokawa
Applied microbiology and biotechnology, 108, 1, 102, 102, Dec. 2024, [International Magazine]
English, Scientific journal, Bacteria belonging to the genus Algoriphagus have been isolated from various sources, such as Antarctic sea ice, seawater, and sediment, and some strains are known to produce orange to red pigments. However, the pigment composition and biosynthetic genes have not been fully elucidated. A new red-pigmented Algoriphagus sp. strain, oki45, was isolated from the surface of seaweed collected from Senaga-Jima Island, Okinawa, Japan. Genome comparison revealed oki45's average nucleotide identity of less than 95% to its closely related species, Algoriphagus confluentis NBRC 111222 T and Algoriphagus taiwanensis JCM 19755 T. Comprehensive chemical analyses of oki45's pigments, including 1H and 13C nuclear magnetic resonance and circular dichroism spectroscopy, revealed that the pigments were mixtures of monocyclic carotenoids, (3S)-flexixanthin ((3S)-3,1'-dihydroxy-3',4'-didehydro-1',2'-dihydro-β,ψ-caroten-4-one) and (2R,3S)-2-hydroxyflexixanthin ((2R,3S)-2,3,1'-trihydroxy-3',4'-didehydro-1',2'-dihydro-β,ψ-caroten-4-one); in particular, the latter compound was new and not previously reported. Both monocyclic carotenoids were also found in A. confluentis NBRC 111222 T and A. taiwanensis JCM 19755 T. Further genome comparisons of carotenoid biosynthetic genes revealed the presence of eight genes (crtE, crtB, crtI, cruF, crtD, crtYcd, crtW, and crtZ) for flexixanthin biosynthesis. In addition, a crtG homolog gene encoding 2,2'-β-hydroxylase was found in the genome of the strains oki45, A. confluentis NBRC 111222 T, and A. taiwanensis JCM 19755 T, suggesting that the gene is involved in 2-hydroxyflexixanthin synthesis via 2-hydroxylation of flexixanthin. These findings expand our knowledge of monocyclic carotenoid biosynthesis in Algoriphagus bacteria. KEY POINTS: • Algoriphagus sp. strain oki45 was isolated from seaweed collected in Okinawa, Japan. • A novel monocyclic carotenoid 2-hydroxyflexixanthin was identified from strain oki45. • Nine genes for 2-hydroxyflexixanthin biosynthesis were found in strain oki45 genome.

Hydrogenimonas leucolamina sp. nov., a hydrogen- and sulphur-oxidizing mesophilic chemolithoautotroph isolated from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Western Pacific Ocean
Shuya Hatakeyama; Sayaka Mino; Mana Mizobata; Mako Takada; Jiro Tsuchiya; Shogo Yamaki; Yasuhiro Ando; Tomoo Sawabe; Ken Takai
International Journal of Systematic and Evolutionary Microbiology, 74, 10, Microbiology Society, 22 Oct. 2024
Scientific journal, A novel mesophilic bacterium, strain SS33^T, was isolated from a deep-sea hydrothermal vent chimney at Suiyo Seamount, Izu-Bonin Arc, Western Pacific Ocean. The cells of strain SS33^T were motile short rods with a single polar flagellum. The growth of strain SS33^T was observed at the temperature range between 33 and 55 °C (optimum growth at 45 °C), at the pH range between 5.0 and 7.1 (optimum growth at pH 6.0) and in the presence of between 2.0 and 4.5% (w/v) NaCl [optimum growth at 3.5% (w/v)]. Strain SS33^T was a facultative anaerobic chemolithoautotroph using molecular hydrogen and elemental sulphur as the sole electron donor. Nitrate, nitrous oxide, sulphate, elemental sulphur and molecular oxygen were capable of serving as the sole electron acceptor. Phylogenetic analysis based on 16S rRNA gene sequences placed strain SS33^T in the genus Hydrogenimonas belonging to the class Epsilonproteobacteria. The closely related species of strain SS33^T were Hydrogenimonas urashimensis SSM-Sur55^T (95.96%), Hydrogenimonas thermophila EP1-55-1%^T (95.75%) and Hydrogenimonas cancrithermarum ISO32^T (95.24%). According to the taxonomic and physiological characteristics, it is proposed that strain SS33^T was classified into a novel species of genus Hydrogenimonas, Hydrogenimonas leucolamina sp. nov., with SS33^T (=JCM 39184^T =KCTC 25253^T) as the type strain. Furthermore, the genome comparison of Epsilonproteobacteria revealed that their [NiFe] hydrogenase genes belonging to Group 1b could be divided into two phylogenetic lineages and suggested that the reverse gyrase gene has been lost after division to the genus Hydrogenimonas.

Time-course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N2O-reducing thermophile
Jiro Tsuchiya; Sayaka Mino; Fuki Fujiwara; Nao Okuma; Yasunori Ichihashi; Robert M. Morris; Brook L. Nunn; Emma Timmins-Schiffman; Tomoo Sawabe
iScience, 111074, 111074, Elsevier BV, Sep. 2024
Scientific journal

The Description of Pseudoalteromonas apostichopi sp. nov., Vibrio apostichopi sp. nov., and Marinobacter apostichopi sp. nov. from the Fertilized Eggs and Larvae of Apostichopus japonicus
Rika Kudo; Ryota Yamano; Juanwen Yu; Shuya Hatakeyama; Chunqi Jiang; Sayaka Mino; Shogo Yamaki; Yasuhiro Ando; Yuichi Sakai; Tomoo Sawabe
Current Microbiology, 81, 8, Springer Science and Business Media LLC, 28 Jun. 2024
Scientific journal

Core Transcriptome of Hydrogen Producing Marine Vibrios Reveals Contribution of Glycolysis in Their Efficient Hydrogen Production.
Yoshihiro Sato; Sayaka Mino; Fabiano Thompson; Tomoo Sawabe
Current microbiology, 81, 8, 230, 230, 19 Jun. 2024, [International Magazine]
English, Scientific journal, Pyruvate (Pyr) is the end product of the glycolysis pathway. Pyr is also renewable and is further metabolized to produce formate, which is the precursor of H2, via pyruvate formate lyase (PFL) under anaerobic conditions. The formate is excluded and re-imported via the formate channel and is then converted to H2 via the formate hydrogenlyase (FHL) complex. In H2 producing marine vibrios, such as Vibrio tritonius and Vibrio porteresiae in the Porteresiae clade of the family Vibrionaceae, apparent but inefficient H2 production from Pyr has been observed. To elucidate the molecular mechanism of why this inefficient H2 production is observed in Pry-metabolized marine vibrio cells and how glycolysis affects those H2 productions of marine vibrios, the "Core Transcriptome" approach to find common gene expressions of those two major H2 producing Vibrio species in Pyr metabolism was first applied. In the Pyr-metabolized vibrio cells, genes for the "Phosphoenolpyruvate (PEP)-Pyruvate-Oxalate (PPO)" node, due to energy saving, and PhoB-, RhaR-, and DeoR-regulons were regulated. Interestingly, a gene responsible for oxalate/formate family antiporter was up-regulated in Pyr-metabolized cells compared to those of Glc-metabolized cells, which provides new insights into the uses of alternative formate exclusion mechanics due to energy deficiencies in Pyr-metabolized marine vibrios cells. We further discuss the contribution of the Embden-Meyerhof-Parnas (EMP) pathway to efficient H2 production in marine vibrios.

Hydrogenimonas cancrithermarum sp. nov., a hydrogen- and thiosulfate-oxidizing mesophilic chemolithoautotroph isolated from diffuse-flow fluids on the East Pacific Rise, and an emended description of the genus Hydrogenimonas
Sayaka Mino; So Fukazawa; Jiro Tsuchiya; Jesse C. McNichol; Stefan M. Sievert; Shogo Yamaki; Yasuhiro Ando; Tomoo Sawabe
International Journal of Systematic and Evolutionary Microbiology, 73, 11, Microbiology Society, 03 Nov. 2023
Scientific journal, A novel mesophilic, hydrogen- and thiosulfate-oxidizing bacterium, strain ISO32^T, was isolated from diffuse-flow hydrothermal fluids from the Crab Spa vent on the East Pacific Rise. Cells of ISO32^T were rods, being motile by means of a single polar flagellum. The isolate grew at a temperature range between 30 and 55 °C (optimum, 43 °C), at a pH range between 5.3 and 7.6 (optimum, pH 5.8) and in the presence of 2.0–4.0 % NaCl (optimum, 2.5 %). The isolate was able to grow chemolithoautotrophically with molecular hydrogen, thiosulfate or elemental sulfur as the sole electron donor. Thiosulfate, elemental sulfur, nitrate and molecular oxygen were each used as a sole electron acceptor. Phylogenetic analysis of 16S rRNA gene sequences placed ISO32^T in the genus Hydrogenimonas of the class Epsilonproteobacteria, with Hydrogenimonas thermophila EP1-55–1 %^T as its closest relative (95.95 % similarity). On the basis of the phylogenetic, physiological and genomic characteristics, it is proposed that the organism represents a novel species within the genus Hydrogenimonas, Hydrogenimonas cancrithermarum sp. nov. The type strain is ISO32^T (=JCM 39185^T =KCTC 25252^T). Furthermore, the genomic properties of members of the genus Hydrogenimonas are distinguished from those of members of other thermophilic genera in the orders Campylobacterales (Nitratiruptor and Nitrosophilus) and Nautiliales (Caminibacter, Nautilia and Lebetimonas), with larger genome sizes and lower 16S rRNA G+C content values. Comprehensive metabolic comparisons based on genomes revealed that genes responsible for the Pta–AckA pathway were observed exclusively in members of mesophilic genera in the order Campylobacterales and of the genus Hydrogenimonas. Our results indicate that the genus Hydrogenimonas contributes to elucidating the evolutionary history of Epsilonproteobacteria in terms of metabolism and transition from a thermophilic to a mesophilic lifestyle.

Unveiling the early life core microbiome of the sea cucumber Apostichopus japonicus and the unexpected abundance of the growth-promoting Sulfitobacter.
Juanwen Yu; Chunqi Jiang; Ryota Yamano; Shotaro Koike; Yuichi Sakai; Sayaka Mino; Tomoo Sawabe
Animal microbiome, 5, 1, 54, 54, 24 Oct. 2023, [International Magazine]
English, Scientific journal, BACKGROUND: Microbiome in early life has long-term effects on the host's immunological and physiological development and its disturbance is known to trigger various diseases in host Deuterostome animals. The sea cucumber Apostichopus japonicus is one of the most valuable marine Deuterostome invertebrates in Asia and a model animal in regeneration studies. To understand factors that impact on host development and holobiont maintenance, host-microbiome association has been actively studied in the last decade. However, we currently lack knowledge of early life core microbiome during its ontogenesis and how it benefits the host's growth. RESULTS: We analyzed the microbial community in 28 sea cucumber samples from a laboratory breeding system, designed to replicate aquaculture environments, across six developmental stages (fertilized eggs to the juvenile stage) over a three years-period to examine the microbiomes' dynamics and stability. Microbiome shifts occurred during sea cucumber larval ontogenesis in every case. Application of the most sophisticated core microbiome extraction methodology, a hybrid approach with abundance-occupancy core microbiome analyses (top 75% of total reads and > 70% occupation) and core index calculation, first revealed early life core microbiome consisted of Alteromonadaceae and Rhodobacteraceae, as well as a stage core microbiome consisting of pioneer core microbe Pseudoalteromonadaceae in A. japonicus, suggesting a stepwise establishment of microbiome related to ontogenesis and feeding behavior in A. japonicus. More interestingly, four ASVs affiliated to Alteromonadaceae and Rhodobacteraceae were extracted as early life core microbiome. One of the ASV (ASV0007) was affiliated to the Sulfitobactor strain BL28 (Rhodobacteraceae), isolated from blastula larvae in the 2019 raring batch. Unexpectedly, a bioassay revealed the BL28 strain retains a host growth-promoting ability. Further meta-pangenomics approach revealed the BL28 genome reads were abundant in the metagenomic sequence pool, in particular, in that of post-gut development in early life stages of A. japonicus. CONCLUSION: Repeated rearing efforts of A. japonicus using laboratory aquaculture replicating aquaculture environments and hybrid core microbiome extraction approach first revealed particular ASVs affiliated to Alteromonadaceae and Rhodobacteraceae as the A. japonicus early life core microbiome. Further bioassay revealed the growth promoting ability to the host sea cucumber in one of the core microbes, the Sulfitobactor strain BL28 identified as ASV0007. Genome reads of the BL28 were abundant in post-gut development of A. japonicus, which makes us consider effective probiotic uses of those core microbiome for sea cucumber resource production and conservation. The study also emphasizes the importance of the core microbiome in influencing early life stages in marine invertebrates. Understanding these dynamics could offer pathways to improve growth, immunity, and disease resistance in marine invertebrates.

A lesson from polybutylene succinate plastisphere to the discovery of novel plastic degrading enzyme genes in marine vibrios.
Yutaro Kimura; Yutaka Fukuda; Rumi Otsu; Juwanen Yu; Sayaka Mino; Satoru Misawa; Satoshi Maruyama; Yuta Ikeda; Remi Miyamachi; Hiroshi Noguchi; Satoshi Kato; Yasuhito Yamamoto; Tomoo Sawabe
Environmental microbiology, 29 Sep. 2023, [International Magazine]
English, Scientific journal, Polybutylene succinate (PBS) is an eco-friendly green plastic. However, PBS was shown as being non-biodegradable in marine environments, and up until now, only a limited number of PBS-degrading marine microbes have been discovered. We first set up in vitro PBS- and PBSA (polybutylene succinate adipate)-plastispheres to characterize novel PBS-degrading marine microbes. Microbial growth and oxygen consumption were observed in both PBS- and PBSA-plastispheres enriched with natural seawater collected from Usujiri, Hokkaido, Japan, and Vibrionaceae and Pseudoalteromonadaceae were significantly enriched on these films. Further gene identification indicated that vibrios belonging to the Gazogenes clade possess genes related to a PBS degrading enzyme (PBSase). The PBS degradation assay for six Gazogenes clade vibrios identified Vibrio ruber, Vibrio rhizosphaerae, and Vibrio spartinae as being capable of degrading PBS. We further identified the gene responsible for PBSase from the type strain of V. ruber, and the purified recombinant vibrio PBSase was found to have low-temperature adaptation and was active under high NaCl concentrations. We also provided docking models between the vibrio PBSase and PBS and PBSA units to show how vibrio PBSase interacts with each substrate compared to the Acidovorax PBSase. These results could contribute to a more sustainable society through further utilization of PBS in marine environments and plastic recycling.

Genome taxonomy of the genus Thalassotalea and proposal of Thalassotalea hakodatensis sp. nov. isolated from sea cucumber larvae.
Ryota Yamano; Juanwen Yu; Alfabetian Harjuno Condro Haditomo; Chunqi Jiang; Sayaka Mino; Jesús L Romalde; Kyuhee Kang; Yuichi Sakai; Tomoo Sawabe
PloS one, 18, 6, e0286693, 2023, [International Magazine]
English, Scientific journal, The genus Thalassotalea is ubiquitous in marine environments, and up to 20 species have been described so far. A Gram-staining-negative, aerobic bacterium, designated strain PTE2T was isolated from laboratory-reared larvae of the Japanese sea cucumber Apostichopus japonicus. Phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PTE2T was closely related to Thalassotalea sediminis N211T (= KCTC 42588T = MCCC 1H00116T) with 97.9% sequence similarity. ANI and in silico DDH values against Thalassotalea species were 68.5-77.0% and 19.7-24.6%, respectively, indicating the novelty of PTE2T. Based on genome-based taxonomic approaches, strain PTE2T (= JCM 34608T = KCTC 82592T) is proposed as a new species, Thalassotalea hakodatensis sp. nov.

Genome taxonomy of the genus Neptuniibacter and proposal of Neptuniibacter victor sp. nov. isolated from sea cucumber larvae.
Rika Kudo; Ryota Yamano; Juanwen Yu; Shotaro Koike; Alfabetian Harjuno Condro Haditomo; Mayanne A M de Freitas; Jiro Tsuchiya; Sayaka Mino; Fabiano Thompson; Jesús L Romalde; Hisae Kasai; Yuichi Sakai; Tomoo Sawabe
PloS one, 18, 8, e0290060, 2023, [International Magazine]
English, Scientific journal, A Gram-staining-negative, oxidase-positive, strictly aerobic rod-shaped bacterium, designated strain PT1T, was isolated from the laboratory-reared larvae of the sea cucumber Apostichopus japonicus. A phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PT1T was closely related to Neptuniibacter marinus ATR 1.1T (= CECT 8938T = DSM 100783T) and Neptuniibacter caesariensis MED92T (= CECT 7075T = CCUG 52065T) showing 98.2% and 98.1% sequence similarity, respectively. However, the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values among these three strains were 72.0%-74.8% and 18.3%-19.5% among related Neptuniibacter species, which were below 95% and 70%, respectively, confirming the novel status of PT1T. The average amino acid identity (AAI) values of PT1T showing 74-77% among those strains indicated PT1T is a new species in the genus Neptuniibacter. Based on the genome-based taxonomic approach, Neptuniibacter victor sp. nov. is proposed for PT1T. The type strain is PT1T (JCM 35563T = LMG 32868T).

Nitrosophilus kaiyonis sp. nov., a hydrogen-, sulfur- and thiosulfate-oxidizing chemolithoautotroph within "Campylobacteria" isolated from a deep-sea hydrothermal vent in the Mid-Okinawa Trough.
So Fukazawa; Sayaka Mino; Jiro Tsuchiya; Satoshi Nakagawa; Ken Takai; Tomoo Sawabe
Archives of microbiology, 205, 1, 12, 12, 03 Dec. 2022, [International Magazine]
English, Scientific journal, A novel bacterium, strain MOT50T, was isolated from the chimney structure at the Iheya North field in the Mid-Okinawa Trough. The cells were motile short rods with a single polar flagellum. Growth was observed between 40 and 65 ℃ (optimum, 52 ℃), at pH values between 5.0 and 7.1 (optimum, pH 6.1) and in the presence of 2.0-4.0% NaCl (optimum, 2.5%). The isolates utilized molecular hydrogen, thiosulfate, or elemental sulfur as the sole electron donor. Thiosulfate, elemental sulfur, nitrate, and molecular oxygen are utilized as the sole electron acceptor. Ammonium is required as a nitrogen source. Thiosulfate, elemental sulfur, sulfate, or sulfite serves as a sulfur source for growth. The G + C content of the genomic DNA was 28.9%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain MOT50T belonged to the genus Nitrosophilus of the class "Campylobacteria", and its closest relative was Nitrosophilus labii HRV44T (97.20%). On the basis of the phylogenetic, physiological, and molecular characteristics, it is proposed that the organism represents a novel species within the genus Nitrosophilus, Nitrosophilus kaiyonis sp. nov. The type strain is MOT50T (= JCM 39187T = KCTC 25251T).

Light response of Vibrio parahaemolyticus
Yunato Kuroyanagi; Jiro Tsuchiya; Chunqi Jiang; Sayaka Mino; Hisae Kasai; Daisuke Motooka; Tetsuya Iida; Masataka Satomi; Tomoo Sawabe
Frontiers in Marine Science, 9, Frontiers Media SA, 10 Nov. 2022, [Corresponding author]
Scientific journal

Comparative Physiology and Genomics of Hydrogen-Producing Vibrios
Yuta Matsumura; Kazumich Sato; Chunqi Jiang; Sayaka Mino; Tomoo Swabe
Current Microbiology, 79, 12, Springer Science and Business Media LLC, Nov. 2022, [Corresponding author]
Scientific journal

Draft genome sequence data of Vibrio harveyi VH1 isolated from a diseased tiger grouper, Epinephelus fuscoguttatus, cultured in Malaysia.
Md Ali Amatul-Samahah; Aslah Mohamad; Nurhidayu Al-Saari; Mohd Zamri-Saad; Mohamad Noor Amal Azmai; Mohd Termizi Yusof; Md Yasin Ina-Salwany; Mami Tanaka; Sayaka Mino; Tomoo Sawabe
Data in brief, 44, 108533, 108533, Oct. 2022, [International Magazine]
English, Scientific journal, Vibriosis accounts for 66.7% of diseases reported in groupers' cultures and affects almost all stages of growth. The disease could lead up to mortality up to 50% mortality, and it was reported that high stocking density and poor fish handling were among the factors that contributed to the disease dissemination. V. harveyi has been reported to be among the causative agent and has caused acute mortality in cage groupers. In this study, we report the genome of V. harveyi VH1 isolated from a diseased tiger grouper Epinephelus fuscoguttatus, reared in a cage farm located in the coastal area of Langkawi.

The pan-genome of Splendidus clade species in the family Vibrionaceae: insights into evolution, adaptation, and pathogenicity.
Chunqi Jiang; Hisae Kasai; Sayaka Mino; Jesús L Romalde; Tomoo Sawabe
Environmental microbiology, 24, 10, 4587, 4606, 15 Sep. 2022, [International Magazine]
English, Scientific journal, The Splendidus clade is the largest clade in Vibrionaceae, and its members are often related to mortality of marine animals with huge economic losses. The molecular bases of their pathogenicity and virulence, however, remain largely unknown. In particular, the complete genome sequences of the Splendidus clade species are rarely registered, which is one of the obstacles to predict core and/or unique genes responsible to their adaptation and pathogenicity, and to perform a fine scale meta-transcriptome during bacterial infection to their hosts. In this study, we obtained the complete genomes of all type strains in the Splendidus clade and revealed that 1) different genome sizes (4.4-5.9 Mb) with V. lentus the biggest and most of them had several big plasmids, likely because of the different features on mobilome elements, 2) the Splendidus clade consists of 19 species except V. cortegadensis, and 3 sub-clades (SC) were defined with the 15 most closely related members as SC1; 3) different carbohydrate degradation preferences may be the result of environmental adaptation, 4) a broad prediction of virulence factors (VFs) revealed core and species unique VF genes. This article is protected by copyright. All rights reserved.

Genomic Analyses of Halioticoli Clade Species in Vibrionaceae Reveal Genome Expansion With More Carbohydrate Metabolism Genes During Symbiotic to Planktonic Lifestyle Transition
Chunqi Jiang; Sayaka Mino; Tomoo Sawabe
Frontiers in Marine Science, 9, Frontiers Media SA, 23 Mar. 2022, [Corresponding author]
Scientific journal, Vibrionaceae is one of the most diverse bacterial families and is currently classified into over 50 clades, some members of which play an important role in the symbiotic relationships with humans and animals. Halioticoli clade, which currently consists of 10 species: 8 species associated with the gut of abalone (symbiotic), 1 species (V. breoganii) from bivalves, and 1 species (V. ishigakensis) from subtropical seawater (planktonic). To accelerate studies in the evolution, ecogenomics, and biotechnology of Halioticoli clade species, the genomic backbones and pangenome analyses based on complete genome sequences are needed. Genome sizes of Halioticoli clade species ranged from 3.5 Mb to 4.8 Mb, with V. ishigakensis the biggest. The evolutionary relationships using multilocus sequence analysis based on eight housekeeping genes and 125 single-copy core genes revealed a division of five sub-clades in this clade; 1) V. breoganii, V. comitans, V. inusitatus and V. superstes, 2) V. ezurae, V. neonatus, and V. halioticoli, 3) V. rarus, 4) V. gallicus, and 5) V. ishigakensis. The pan-genomic analysis combined with function and metabolism estimations showed that the planktonic group (sub-clade 5) contained the greatest number of specific genes, and more genes responsible for carbohydrate metabolisms, especially the genes encoding D-galactonate degradation. These results demonstrated that the genome expanded by acquiring more abilities for utilizing various carbohydrates during the evolution from symbiotic to a planktonic lifestyle. Moreover, according to Carbohydrate-Active enZYmes (CAZy) profiling, genes encoding alginate degrading enzymes (aly), classified into PL6, PL7, PL15, and PL17 were common in the ten genomes, but sub-clade 1 had the most. Meanwhile, sub-clade 1and 5 also possessed abundant genes related to macroalgae substrates degradation (GHs), which are also responsible for the genome expansion of sub-clade 1 and 5.

Taxonomic revision of the genus Amphritea supported by genomic and in silico chemotaxonomic analyses, and the proposal of Aliamphritea gen. nov.
Ryota Yamano; Juanwen Yu; Chunqi Jiang; Alfabetian Harjuno Condro Haditomo; Sayaka Mino; Yuichi Sakai; Tomoo Sawabe
PloS one, 17, 8, e0271174, 2022, [International Magazine]
English, Scientific journal, A Gram-staining-negative, aerobic bacterium, designated strain PT3T was isolated from laboratory-reared larvae of the Japanese sea cucumber Apostichopus japonicus. Phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PT3T was closely related to Amphritea ceti RA1T (= KCTC 42154T = NBRC 110551T) and Amphritea spongicola MEBiC05461T (= KCCM 42943T = JCM 16668T) both with 98.3% sequence similarity, however, average nucleotide identity (ANI) and in silico DNA-DNA hybridization (in silico DDH) values among these three strains were below 95% and 70%, respectively, confirming the novelty of PT3T. Furthermore, the average amino acid identity (AAI) values of PT3T against other Amphritea species were on the reported genus delineation boundary (64-67%). Multilocus sequence analysis using four protein-coding genes (recA, mreB, rpoA, and topA) further demonstrated that PT3T, Amphritea ceti and Amphritea spongicola formed a monophyletic clade clearly separate from other members of the genus Amphritea. Three strains (PT3T, A. ceti KCTC 42154T and A. spongicola JCM 16668T) also showed higher similarities in their core genomes compared to those of the other Amphritea spp. Based on the genome-based taxonomic approach, Aliamphritea gen. nov. was proposed together with the reclassification of the genus Amphritea and Aliamphritea ceti comb. nov. (type strain RA1T = KCTC 42154T = NBRC 110551T), Aliamphritea spongicola comb. nov. (type strain MEBiC05461T = KCCM 42943T = JCM 16668T), and Aliamphritea hakodatensis sp. nov. (type strain PT3T = JCM 34607T = KCTC 82591T) were suggested.

Characterization of Microbiomes Associated With the Early Life Stages of Sea Cucumber Apostichopus japonicus Selenka
Juanwen Yu; Yuichi Sakai; Sayaka Mino; Tomoo Sawabe
FRONTIERS IN MARINE SCIENCE, 9, Jan. 2022
English, Scientific journal

The Structure and Function of Gut Microbiomes of Two Species of Sea Urchins, Mesocentrotus nudus and Strongylocentrotus intermedius, in Japan
Alfabetian Harjuno Condro Haditomo; Masanori Yonezawa; Juanwen Yu; Sayaka Mino; Yuichi Sakai; Tomoo Sawabe
Frontiers in Marine Science, 8, Frontiers Media SA, 22 Dec. 2021, [Peer-reviewed], [Corresponding author]
Scientific journal, Sea urchin is an indicator of coastal environmental changes in the global warming era, and is also a model organism in developmental biology and evolution. Due to the depletion of wild resources, new aquaculture techniques for improving stocks have been well studied. The gut microbiome shapes various aspects of a host’s physiology. However, these microbiome structures and functions on sea urchins, particularly Mesocentrotus nudus and Strongylocentrotus intermedius which are important marine bioresources commonly found in Japan, have not been fully investigated yet. Using metagenomic approaches including meta16S and shotgun metagenome sequencings, the structures, functions, and dynamics of the gut microbiome of M. nudus and S. intermedius, related to both habitat environment and host growth, were studied. Firstly, a broad meta16S analysis revealed that at the family level, Psychromonadaceae and Flavobacteriaceae reads (38–71%) dominated in these sea urchins, which is a unique feature observed in species in Japan. Flavobacteriaceae reads were more abundant in individuals after rearing in an aquarium with circulating compared to one with running water. Campylobacteraceae and Vibrionaceae abundances increased in both kinds of laboratory-reared sea urchins in both types of experiments. 2-weeks feeding experiments of M. nudus and S. intermedius transplanted from the farm to laboratory revealed that these gut microbial structures were affected by diet rather than rearing environments and host species. Secondly, further meta16S analysis of microbial reads related to M. nudus growth revealed that at least four Amplicon Sequence Variant (ASV) affiliated to Saccharicrinis fermentans, which is known to be a nitrogen (N₂) fixing bacterium, showed a significant positive correlation to the body weight and test diameter. Interestingly, gut microbiome comparisons using shotgun metagenome sequencing of individuals showing higher and lower growth rates revealed a significant abundance of “Nitrate and nitrite ammonification” genes in the higher-grown individuals under the circulating water rearing. These findings provide new insights on the structure-function relationship of sea urchin gut microbiomes beyond previously reported nitrogen fixation function in sea urchin in 1950s; we discovered a nitrate reduction function into ammonium for the growth promotion of sea urchin.

Vibrio Clade 3.0: New Vibrionaceae Evolutionary Units Using Genome-Based Approach.
Chunqi Jiang; Mami Tanaka; Sayo Nishikawa; Sayaka Mino; Jesús L Romalde; Fabiano L Thompson; Bruno Gomez-Gil; Tomoo Sawabe
Current microbiology, 79, 1, 10, 10, 14 Dec. 2021, [International Magazine]
English, Scientific journal, Currently, over 190 species in family Vibrionaceae, including not-yet-cultured taxa, have been described and classified into over nine genera, in which the number of species has doubled compared to the previous vibrio evolutionary update (Vibrio Clade 2.0) (Sawabe et al. 2014). In this study, "Vibrio Clade 3.0," the second update of the molecular phylogenetic analysis was performed based on nucleotide sequences of eight housekeeping genes (8-HKGs) retrieved from genome sequences, including 22 newly determined genomes. A total of 51 distinct clades were observed, of which 21 clades are newly described. We further evaluated the delineation powers of the clade classification based on nucleotide sequences of 34 single-copy genes and 11 ribosomal protein genes (11-RPGs) retrieved from core-genome sequences; however, the delineation power of 8-HKGs is still high and that gene set can be reliably used for the classification and identification of Vibrionaceae. Furthermore, the 11-RPGs set proved to be useful in identifying uncultured species among metagenome-assembled genome (MAG) and/or single-cell genome-assembled genome (SAG) pools. This study expands the awareness of the diversity and evolutionary history of the family Vibrionaceae and accelerates the taxonomic applications in classifying as not-yet-cultured taxa among MAGs and SAGs.

Genetic Environment Surrounding blaOXA-55-like in Clinical Isolates of Shewanella algae Clade and Enhanced Expression of blaOXA-55-like in a Carbapenem-Resistant Isolate.
Yuki Ohama; Kotaro Aoki; Sohei Harada; Tatsuya Nagasawa; Tomoo Sawabe; Lisa Nonaka; Kyoji Moriya; Yoshikazu Ishii; Kazuhiro Tateda
mSphere, 6, 5, e0059321, 27 Oct. 2021, [International Magazine]
English, Scientific journal, Although Shewanella spp. are most frequently isolated from marine environments; more rarely, they have been implicated in human infections. Shewanella spp. are also recognized as the origin of genes for carbapenem-hydrolyzing class D β-lactamases. Due to the spread globally among Enterobacterales in recent years, risk assessments of both clinical and environmental Shewanella strains are urgently needed. In this study, we analyzed the whole-genome sequences of 10 clinical isolates and 13 environmental isolates of Shewanella spp. and compared them with those of Shewanella species strains registered in public databases. In addition, the levels of blaOXA-55-like transcription and β-lactamase activity of a carbapenem-resistant Shewanella algae isolate were compared with those of carbapenem-susceptible S. algae clade isolates. All clinical isolates were genetically identified as S. algae clade (S. algae, Shewanella chilikensis, and Shewanella carassii), whereas all but one of the environmental isolates were identified as various Shewanella spp. outside the S. algae clade. Although all isolates of the S. algae clade commonly possessed an approximately 12,500-bp genetic region harboring blaOXA-55-like, genetic structures outside this region were different among species. Among S. algae clade isolates, only one showed carbapenem resistance, and this isolate showed a high level of blaOXA-55-like transcription and β-lactamase activity. Although this study documented the importance of the S. algae clade in human infections and the relationship between enhanced production of OXA-55-like and resistance to carbapenems in S. algae, further studies are needed to elucidate the generalizability of these findings. IMPORTANCE Shewanella spp., which are known to carry chromosomally located blaOXA genes, have mainly been isolated from marine environments; however, they can also cause infections in humans. In this study, we compared the molecular characteristics of clinical isolates of Shewanella spp. with those originating from environmental sources. All 10 clinical isolates were genetically identified as members of the Shewanella algae clade (S. algae, S. chilikensis, and S. carassii); however, all but one of the 13 environmental isolates were identified as Shewanella species members outside the S. algae clade. Although all the S. algae clade isolates possessed an approximately 12,500-bp genetic region harboring blaOXA-55-like, only one isolate showed carbapenem resistance. The carbapenem-resistant isolate showed a high level of blaOXA-55-like transcription and β-lactamase activity compared with the carbapenem-susceptible isolates. To confirm the clinical significance and antimicrobial resistance mechanisms of the S. algae clade members, analysis involving more clinical isolates should be performed in the future.

Hydrogenimonas urashimensis sp. nov., a hydrogen-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal vent in the Southern Mariana Trough.
Sayaka Mino; Taiki Shiotani; Satoshi Nakagawa; Ken Takai; Tomoo Sawabe
Systematic and applied microbiology, 44, 1, 126170, 126170, Jan. 2021, [International Magazine]
English, Scientific journal

An annual faecal 16S amplicon sequencing of individual sea cucumber (Apostichopus japonicus) demonstrates the feeding behaviours against eukaryotes in natural environments
Yohei Yamazaki; Yuichi Sakai; Sayaka Mino; Tomoo Sawabe
AQUACULTURE RESEARCH, 51, 9, 3602, 3608, Sep. 2020
English, Scientific journal

Biogeochemical Implications of N2O-Reducing Thermophilic Campylobacteria in Deep-Sea Vent Fields, and the Description of Nitratiruptor labii sp. nov.
Muneyuki Fukushi; Sayaka Mino; Hirohisa Tanaka; Satoshi Nakagawa; Ken Takai; Tomoo Sawabe
iScience, 23, 9, 101462, 101462, 15 Aug. 2020, [International Magazine]
English, Scientific journal

Ecogenomics of the Marine Benthic Filamentous Cyanobacterium Adonisia
Juline M. Walter; Felipe H. Coutinho; Luciana Leomil; Paulo I. Hargreaves; Mariana E. Campeao; Veronica V. Vieira; Beatriz S. Silva; Giovana O. Fistarol; Paulo S. Salomon; Tomoo Sawabe; Sayaka Mino; Masashi Hosokawa; Hideaki Miyashita; Fumito Maruyama; Marcel C. van Verk; Bas E. Dutilh; Cristiane C. Thompson; Fabiano L. Thompson
MICROBIAL ECOLOGY, 80, 2, 249, 265, Aug. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Genomic characterization of closely related species in the Rumoiensis clade infers ecogenomic signatures to non-marine environments
Mami Tanaka; Daiki Kumakura; Sayaka Mino; Hidetaka Doi; Yoshitoshi Ogura; Tetsuya Hayashi; Iseo Yumoto; Man Cai; Yu-Guang Zhou; Bruno Gomez-Gil; Toshiyoshi Araki; Tomoo Sawabe
ENVIRONMENTAL MICROBIOLOGY, 22, 8, 3205, 3217, Aug. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Nitrosophilus alvini gen. nov., sp. nov., a hydrogen-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal vent in the East Pacific Rise, inferred by a genome-based taxonomy of the phylum "Campylobacterota".
Taiki Shiotani; Sayaka Mino; Wakana Sato; Sayo Nishikawa; Masanori Yonezawa; Stefan M Sievert; Tomoo Sawabe
PloS one, 15, 12, e0241366, 2020, [International Magazine]
English, Scientific journal

Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration.
Yohei Yamazaki; Yuichi Sakai; Juanwen Yu; Sayaka Mino; Tomoo Sawabe
PeerJ, 8, e10260, 2020, [International Magazine]
English, Scientific journal

Vibrio taketomensis sp. nov. by genome taxonomy
Mami Tanaka; Bi Hongyu; Chunqi Jiang; Sayaka Mino; Pedro Milet Meirelles; Fabiano Thompson; Bruno Gomez-Gil; Tomoo Sawabe
SYSTEMATIC AND APPLIED MICROBIOLOGY, 43, 1, 126048, 126048, Jan. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Repeated selective enrichment process of sediment microbiota occurred in sea cucumber guts
Yohei Yamazaki; Yuichi Sakai; Sayaka Mino; Wataru Suda; Masahira Hattori; Pedro Milet Meirelles; Fabiano Thompson; Tomoo Sawabe
ENVIRONMENTAL MICROBIOLOGY REPORTS, 11, 6, 797, 807, Dec. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
Nor Zulkiply Amalina; Zulperi Dzarifah; Mohammad Noor Azmai Amal; Mohd Termizi Yusof; Mohd Zamri-Saad; Nurhidayu Al-saari; Mami Tanaka; Sayaka Mino; Tomoo Sawabe; Md Yasin Ina-Salwany
Aquaculture Research, 50, 11, 3202, 3210, Blackwell Publishing Ltd, 01 Nov. 2019
English, Scientific journal

Vibriosis in Fish: A Review on Disease Development and Prevention.
M Y Ina-Salwany; Nurhidayu Al-Saari; Aslah Mohamad; Fathin-Amirah Mursidi; Aslizah Mohd-Aris; M N A Amal; Hisae Kasai; Sayaka Mino; Tomoo Sawabe; M Zamri-Saad
Journal of aquatic animal health, 31, 1, 3, 22, Mar. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Current growth in aquaculture production is parallel with the increasing number of disease outbreaks, which negatively affect the production, profitability, and sustainability of the global aquaculture industry. Vibriosis is among the most common diseases leading to massive mortality of cultured shrimp, fish, and shellfish in Asia. High incidence of vibriosis can occur in hatchery and grow-out facilities, but juveniles are more susceptible to the disease. Various factors, particularly the source of fish, environmental factors (including water quality and farm management), and the virulence factors of Vibrio, influence the occurrence of the disease. Affected fish show weariness, with necrosis of skin and appendages, leading to body malformation, slow growth, internal organ liquefaction, blindness, muscle opacity, and mortality. A combination of control measures, particularly a disease-free source of fish, biosecurity of the farm, improved water quality, and other preventive measures (e.g., vaccination) might be able to control the infection. Although some control measures are expensive and less practical, vaccination is effective, relatively cheap, and easily implemented. In this review, the latest knowledge on the pathogenesis and control of vibriosis, including vaccination, is discussed.

Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius.
Nurhidayu Al-Saari; Eri Amada; Yuta Matsumura; Mami Tanaka; Sayaka Mino; Tomoo Sawabe
PeerJ, 7, e6769, 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Biohydrogen is one of the most suitable clean energy sources for sustaining a fossil fuel independent society. The use of both land and ocean bioresources as feedstocks show great potential in maximizing biohydrogen production, but sodium ion is one of the main obstacles in efficient bacterial biohydrogen production. Vibrio tritonius strain AM2 can perform efficient hydrogen production with a molar yield of 1.7 mol H2/mol mannitol, which corresponds to 85% theoretical molar yield of H2 production, under saline conditions. With a view to maximizing the hydrogen production using marine biomass, it is important to accumulate knowledge on the effects of salts on the hydrogen production kinetics. Here, we show the kinetics in batch hydrogen production of V. tritonius strain AM2 to investigate the response to various NaCl concentrations. The modified Han-Levenspiel model reveals that salt inhibition in hydrogen production using V. tritonius starts precisely at the point where 10.2 g/L of NaCl is added, and is critically inhibited at 46 g/L. NaCl concentration greatly affects the substrate consumption which in turn affects both growth and hydrogen production. The NaCl-dependent behavior of fermentative hydrogen production of V. tritonius compared to that of Escherichia coli JCM 1649 reveals the marine-adapted fermentative hydrogen production system in V. tritonius. V. tritonius AM2 is capable of producing hydrogen from seaweed carbohydrate under a wide range of NaCl concentrations (5 to 46 g/L). The optimal salt concentration producing the highest levels of hydrogen, optimal substrate consumption and highest molar hydrogen yield is at 10 g/L NaCl (1.0% (w/v)).

Availability of Nanopore sequences in the genome taxonomy for Vibrionaceae systematics: Rumoiensis Glade species as a test case
Mami Tanaka; Sayaka Mino; Yoshitoshi Ogura; Tetsuya Hayashi; Tomoo Sawabe
PEERJ, 6, e5018, Jun. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Corrigendum to "Thaumasiovibrio occultus gen. nov. sp. nov. and Thaumasiovibrio subtropicus sp. nov. within the family Vibrionaceae, isolated from coral reef seawater off Ishigaki Island, Japan" [Syst. Appl. Microbiol. 40 (5) (2017) 290-296].
A K M Rohul Amin; Mami Tanaka; Nurhidayu Al-Saari; Gao Feng; Sayaka Mino; Yoshitoshi Ogura; Tetsuya Hayashi; Pedro M Meirelles; Fabiano L Thompson; Bruno Gomez-Gil; Toko Sawabe; Tomoo Sawabe
Systematic and applied microbiology, 41, 1, 62, 63, Jan. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Enrichment and Genomic Characterization of a N2O-Reducing Chemolithoautotroph From a Deep-Sea Hydrothermal Vent.
Sayaka Mino; Naoki Yoneyama; Satoshi Nakagawa; Ken Takai; Tomoo Sawabe
Frontiers in bioengineering and biotechnology, 6, 184, 184, 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Nitrous oxide (N2O) is a greenhouse gas and also leads to stratospheric ozone depletion. In natural environments, only a single N2O sink process is the microbial reduction of N2O to N2, which is mediated by nitrous oxide reductase (NosZ) encoded by nosZ gene. The nosZ phylogeny has two distinct clades, clade I and formerly overlooked clade II. In deep-sea hydrothermal environments, several members of the class Campylobacteria are shown to harbor clade II nosZ gene and perform the complete denitrification of nitrate to N2; however, little is known about their ability to grow on exogenous N2O as the sole electron acceptor. Here, we obtained an enrichment culture from a deep-sea hydrothermal vent in the Southern Mariana Trough, which showed a respiratory N2O reduction with H2 as an electron donor. The single amplicon sequence variant (ASV) presenting 90% similarity to Hydrogenimonas species within the class Campylobacteria was predominant throughout the cultivation period. Metagenomic analyses using a combination of short-read and long-read sequence data succeeded in reconstructing a complete genome of the dominant ASV, which encoded clade II nosZ gene. This study represents the first cultivation analysis that shows the occurrence of N2O-respiring microorganisms in a deep-sea hydrothermal vent and provides the opportunity to assess their capability to reduce N2O emission from the environments.

Metagenomics of Coral Reefs Under Phase Shift and High Hydrodynamics.
Pedro Milet Meirelles; Ana Carolina Soares; Louisi Oliveira; Luciana Leomil; Luciana Reis Appolinario; Ronaldo Bastos Francini-Filho; Rodrigo Leão de Moura; Renato Tenan de Barros Almeida; Paulo S Salomon; Gilberto Menezes Amado-Filho; Ricardo Kruger; Eduardo Siegle; Diogo A Tschoeke; Isao Kudo; Sayaka Mino; Tomoo Sawabe; Cristiane C Thompson; Fabiano L Thompson
Frontiers in microbiology, 9, 2203, 2203, 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Local and global stressors have affected coral reef ecosystems worldwide. Switches from coral to algal dominance states and microbialization are the major processes underlying the global decline of coral reefs. However, most of the knowledge concerning microbialization has not considered physical disturbances (e.g., typhoons, waves, and currents). Southern Japan reef systems have developed under extreme physical disturbances. Here, we present analyses of a three-year investigation on the coral reefs of Ishigaki Island that comprised benthic and fish surveys, water quality analyses, metagenomics and microbial abundance data. At the four studied sites, inorganic nutrient concentrations were high and exceeded eutrophication thresholds. The dissolved organic carbon (DOC) concentration (up to 233.3 μM) and microbial abundance (up to 2.5 × 105 cell/mL) values were relatively high. The highest vibrio counts coincided with the highest turf cover (∼55-85%) and the lowest coral cover (∼4.4-10.2%) and fish biomass (0.06 individuals/m2). Microbiome compositions were similar among all sites and were dominated by heterotrophs. Our data suggest that a synergic effect among several regional stressors are driving coral decline. In a high hydrodynamics reef environment, high algal/turf cover, stimulated by eutrophication and low fish abundance due to overfishing, promote microbialization. Together with crown-of-thorns starfish (COTS) outbreaks and possible of climate changes impacts, theses coral reefs are likely to collapse.

Corrigendum to "Vibrio ishigakensis sp. nov., in Halioticoli clade isolated from seawater in Okinawa coral reef area, Japan" [Syst. Appl. Microbiol. 39 (5) (2016) 330-335].
Feng Gao; Nurhidayu Al-Saari; A K M Rohul Amin; Kazumichi Sato; Sayaka Mino; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Moriya Ohkuma; Paulo Iiboshi Hargreaves; Pedro Milet Meirelles; Fabiano L Thompson; Cristiane Thompson; Bruno Gomez-Gil; Toko Sawabe; Tomoo Sawabe
Systematic and applied microbiology, 40, 8, 516, 516, Dec. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed (vol 12, e0180053, 2017)
Mami Tanaka; Shoko Endo; Fumihito Kotake; Nurhidayu Al-Saari; A. K. M. Rohul Amin; Gao Feng; Sayaka Mino; Hidetaka Doi; Yoshitoshi Ogura; Tetsuya Hayashi; Wataru Suda; Masahira Hattori; Isao Yumoto; Toko Sawabe; Tomoo Sawabe; Toshiyoshi Araki
PLOS ONE, 12, 12, e0189555, Dec. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Thaumasiovibrio occultus gen. nov. sp. nov. and Thaumasiovibrio subtropicus sp. nov. within the family Vibrionaceae, isolated from coral reef seawater off Ishigaki Island, Japan.
A K M Rohul Amin; Mami Tanaka; Nurhidayu Al-Saari; Gao Feng; Sayaka Mino; Yoshitoshi Ogura; Tetsuya Hayashi; Pedro M Meirelles; Fabiano L Thompson; Bruno Gomez-Gil; Toko Sawabe; Tomoo Sawabe
Systematic and applied microbiology, 40, 5, 290, 296, Jul. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed
Mami Tanaka; Shoko Endo; Fumihito Kotake; Nurhidayu Al-Saari; A. K. M. Rohul Amin; Gao Feng; Sayaka Mino; Hidetaka Doi; Yoshitoshi Ogura; Tetsuya Hayashi; Wataru Suda; Masahira Hattori; Isao Yumoto; Toko Sawabe; Tomoo Sawabe; Toshiyoshi Araki
PLOS ONE, 12, 6, e0180053, Jun. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Aeromonadaceae, Vibrionaceae and related organisms  Minutes of the meetings, 31 March 2016, Roscoff, France.
Tomoo Sawabe; James D Oliver
International journal of systematic and evolutionary microbiology, 67, 3, 759, 760, Apr. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Endemicity of the cosmopolitan mesophilic chemolithoautotroph Sulfurimonas at deep-sea hydrothermal vents.
Sayaka Mino; Satoshi Nakagawa; Hiroko Makita; Tomohiro Toki; Junichi Miyazaki; Stefan M Sievert; Martin F Polz; Fumio Inagaki; Anne Godfroy; Shingo Kato; Hiromi Watanabe; Takuro Nunoura; Koichi Nakamura; Hiroyuki Imachi; Tomo-O Watsuji; Shigeaki Kojima; Ken Takai; Tomoo Sawabe
The ISME journal, 11, 4, 909, 919, Apr. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Correction: Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed.
Mami Tanaka; Shoko Endo; Fumihito Kotake; Nurhidayu Al-Saari; A K M Rohul Amin; Gao Feng; Sayaka Mino; Hidetaka Doi; Yoshitoshi Ogura; Tetsuya Hayashi; Wataru Suda; Masahira Hattori; Isao Yumoto; Toko Sawabe; Tomoo Sawabe; Toshiyoshi Araki
PloS one, 12, 12, e0189555, 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Microbial biofuels: Potential and challenges
Nurhidayu Al-saari; Tomoo Sawabe; Yuta Matsumura; Kazumichi Sato; Sayaka Mino; Toko Sawabe
Molecular Diversity of Environmental Prokaryotes, 285, 294, CRC Press, 19 Aug. 2016
English, In book

Vibrio ishigakensis sp. nov., in Halioticoli clade isolated from seawater in Okinawa coral reef area, Japan.
Feng Gao; Nurhidayu Al-Saari; A K M Rohul Amin; Kazumichi Sato; Sayaka Mino; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Moriya Ohkuma; Paulo Iiboshi Hargreaves; Pedro Milet Meirelles; Fabiano L Thompson; Cristiane Thompson; Bruno Gomez-Gil; Toko Sawabe; Tomoo Sawabe
Systematic and applied microbiology, 39, 5, 330, 5, Jul. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

First microbiota assessments of children's paddling pool waters evaluated using 16S rRNA gene-based metagenome analysis
Toko Sawabe; Wataru Suda; Kenshiro Ohshima; Masahira Hattori; Tomoo Sawabe
JOURNAL OF INFECTION AND PUBLIC HEALTH, 9, 3, 362, 365, May 2016, [Peer-reviewed]
English, Scientific journal

Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps
Yohei Yamazaki; Pedro Milet Meirelles; Sayaka Mino; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Fabiano L. Thompson; Yuichi Sakai; Toko Sawabe; Tomoo Sawabe
SCIENTIFIC REPORTS, 6, 21631, 21631, Feb. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

The First Temporal and Spatial Assessment of Vibrio Diversity of the Surrounding Seawater of Coral Reefs in Ishigaki, Japan.
A K M R Amin; Gao Feng; Nurhidayu Al-Saari; Pedro M Meirelles; Yohei Yamazaki; Sayaka Mino; Fabiano L Thompson; Toko Sawabe; Tomoo Sawabe
Frontiers in microbiology, 7, 1185, 1185, 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal, Coral reefs perform a major role in regulating marine biodiversity and serve as hotspot for highly dynamic and diverse microbiomes as holobionts. Corals around Ishigaki, however, are at risk due to tremendous stressors including elevation of seawater temperature, eutrophication and so on. However, no information is currently available on how Vibrio diversity fluctuates spatially and temporally due to environmental determinants in Ishigaki coral reef ecosystems. The aim of this study is to elucidate spatiotemporal Vibrio diversity dynamic at both community and population levels and to assess the environmental drivers correlated to Vibrio abundance and diversity. The Vibrio community identified based on pyrH gene phylogeny of 685 isolates from seawater directly connecting to Ishigaki coral holobionts consisted of 22 known and 12 potential novel Vibrionaceae species. The most prominent species were V. hyugaensis, V. owensii and V. harveyi followed by V. maritimus/V. variabillis, V. campbellii, V. coralliilyticus, and Photobacterium rosenbergii. The Vibrio community fluctuations, assessed by PCoA with UniFrac distance and clustering with Euclidiean distance were varied less not only by year but also by site. Interestingly, significant positive correlation was observed between rising seawater temperature and the abundance of V. campbellii (r = 0.62; P < 0.05) whereas the opposite was observed for V. owensii (r = -0.58; P < 0.05) and the C6 group of V. hyugaensis (r = -0.62; P < 0.05). AdaptML-based microhabitat differentiation revealed that V. harveyi, V. campbellii, P. rosenbergii, and V. coralliilyticus populations were less-ecologically distinctive whereas V. astriarenae and V. ishigakensis were ecologically diverse. This knowledge could be important clue for the future actions of coral conservation.

Effects of Hemagglutination Activity in the Serum of a Deep-Sea Vent Endemic Crab, Shinkaia Crosnieri, on Non-Symbiotic and Symbiotic Bacteria
So Fujiyoshi; Hiroaki Tateno; Tomoo Watsuji; Hideyuki Yamaguchi; Daisuke Fukushima; Sayaka Mino; Makoto Sugimura; Tomoo Sawabe; Ken Takai; Shigeki Sawayama; Satoshi Nakagawa
MICROBES AND ENVIRONMENTS, 30, 3, 228, 234, Sep. 2015, [Peer-reviewed]
English, Scientific journal

Identification of a gene cluster responsible for hydrogen evolution in Vibrio tritonius strain AM2 with transcriptional analyses
Yuta Matsumura; Hidayu Al-saari; Sayaka Mino; Satoshi Nakagawa; Fumito Maruyama; Yoshitoshi Ogura; Tetsuya Hayashi; Ken Kurokawa; Toko Sawabe; Tomoo Sawabe
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 40, 30, 9137, 9146, Aug. 2015, [Peer-reviewed]
English, Scientific journal

Advanced Microbial Taxonomy Combined with Genome-Based-Approaches Reveals that Vibrio astriarenae sp nov., an Agarolytic Marine Bacterium, Forms a New Clade in Vibrionaceae
Nurhidayu Al-Saari; Feng Gao; Amin A. K. M. Rohul; Kazumichi Sato; Keisuke Sato; Sayaka Mino; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Moriya Ohkuma; Pedro M. Meirelles; Fabiano L. Thompson; Cristiane Thompson; Gilberto M. A. Filho; Bruno Gomez-Gil; Toko Sawabe; Tomoo Sawabe
PLOS ONE, 10, 8, Aug. 2015, [Peer-reviewed]
English, Scientific journal

Baseline Assessment of Mesophotic Reefs of the Vitoria-Trindade Seamount Chain Based on Water Quality, Microbial Diversity, Benthic Cover and Fish Biomass Data
Pedro M. Meirelles; Gilberto M. Amado-Filho; Guilherme H. Pereira-Filho; Hudson T. Pinheiro; Rodrigo L. de Moura; Jean-Christophe Joyeux; Eric F. Mazzei; Alex C. Bastos; Robert A. Edwards; Elizabeth Dinsdale; Rodolfo Paranhos; Eidy O. Santos; Tetsuya Iida; Kazuyoshi Gotoh; Shota Nakamura; Tomoo Sawabe; Carlos E. Rezende; Luiz M. R. Gadelha; Ronaldo B. Francini-Filho; Cristiane Thompson; Fabiano L. Thompson
PLOS ONE, 10, 6, Jun. 2015, [Peer-reviewed]
English, Scientific journal

Microbial taxonomy in the post-genomic era: Rebuilding from scratch?
Cristiane C. Thompson; Gilda R. Amaral; Mariana Campeao; Robert A. Edwards; Martin F. Polz; Bas E. Dutilh; David W. Ussery; Tomoo Sawabe; Jean Swings; Fabiano L. Thompson
ARCHIVES OF MICROBIOLOGY, 197, 3, 359, 370, Apr. 2015, [Peer-reviewed]
English

Structure of a novel monocyclic carotenoid, 3 ''-hydroxy-2 '-isopentenylsaproxanthin ((3R,2 ' S)-2 -(3-hydroxy-3 -methylbuty1)-3 ', 4 '-didehydro-1 ', 2 '-dihydro-beta, Psi-carotene-3, 1 '-diol), from a flavobacterium Gillisia limnaea strain DSM 15749
Naoki Takatani; Tomoo Sawabe; Takashi Maoka; Kazuo Miyashita; Masashi Hosokawa
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 4, 2, 174, 179, Apr. 2015, [Peer-reviewed]
English, Scientific journal

Microbial community diversity and physical-chemical features of the Southwestern Atlantic Ocean
Nelson Alves Junior; Pedro Milet Meirelles; Eidy de Oliveira Santos; Bas Dutilh; Genivaldo G. Z. Silva; Rodolfo Paranhos; Anderson S. Cabral; Carlos Rezende; Tetsuya Iida; Rodrigo L. de Moura; Ricardo Henrique Kruger; Renato C. Pereira; Rogerio Valle; Tomoo Sawabe; Cristiane Thompson; Fabiano Thompson
ARCHIVES OF MICROBIOLOGY, 197, 2, 165, 179, Mar. 2015, [Peer-reviewed]
English, Scientific journal

Biofuel innovation by microbial diversity
Thiago Bruce; Astria D. Ferrão-Gonzales; Yutaka Nakashimada; Yuta Matsumura; Fabiano Thompson; Tomoo Sawabe
Springer Handbook of Marine Biotechnology, 1163, 1180, Springer Berlin Heidelberg, 01 Jan. 2015, [Peer-reviewed]
English, In book

Corrigendum: Updating the Vibrio clades defined by multilocus sequence phylogeny: proposal of eight new clades, and the description of Vibrio tritonius sp nov.
Tomoo Sawabe; Yoshitoshi Ogura; Yuta Matsumura; Gao Feng; A. K. M. Rohul Amin; Sayaka Mino; Satoshi Nakagawa; Toko Sawabe; Ramesh Kumar; Yohei Fukui; Masataka Satomi; Ryoji Matsushima; Fabiano L. Thompson; Bruno Gomez Gil; Richard Christen; Fumito Maruyama; Ken Kurokawa; Tetsuya Hayashi
FRONTIERS IN MICROBIOLOGY, 5, 583, Nov. 2014, [Peer-reviewed]
English

Current trends in risk management research for ensuring food safety throughout seafood production, processing, and distribution
Tomoo Sawabe; Masataka Satomi; Koji Yamazaki; Shinichi Kawamoto; Masahira Hattori; Wataru Suda; Kageyasu Takanashi; Takashi Sasahira; Takashi Kuda; Hajime Takahashi; Bon Kimura; Masashi Ohtsubo; Kenji Isshiki
NIPPON SUISAN GAKKAISHI, 80, 6, 1002, 1008, Nov. 2014, [Peer-reviewed]
Japanese

Marinobacter salarius sp nov and Marinobacter similis sp nov., Isolated from Sea Water
Hooi Jun Ng; Mario Lopez-Perez; Hayden K. Webb; Daniela Gomez; Tomoo Sawabe; Jason Ryan; Mikhail Vyssotski; Chantal Bizet; Francois Malherbe; Valery V. Mikhailov; Russell J. Crawford; Elena P. Ivanova
PLOS ONE, 9, 9, Sep. 2014, [Peer-reviewed]
English, Scientific journal

Enhanced hydrogen production by a newly described heterotrophic marine bacterium, Vibrio tritonius strain AM2, using seaweed as the feedstock
Yuta Matsumura; Kazumichi Sato; Nurhidayu Al-Saari; Satoshi Nakagawa; Tomoo Sawabe
International Journal of Hydrogen Energy, 39, 14, 7270, 7277, Elsevier Ltd, 05 May 2014, [Peer-reviewed]
English, Scientific journal

Zunongwangia mangrovi sp nov., isolated from mangrove (Avicennia marina) rhizosphere, and emended description of the genus Zunongwangia
N. Rameshkumar; Ramya Krishnan; Elke Lang; Yuta Matsumura; Toko Sawabe; Tomoo Sawabe
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64, 545, 550, Feb. 2014, [Peer-reviewed]
English, Scientific journal

Allying with armored snails: the complete genome of gammaproteobacterial endosymbiont
Satoshi Nakagawa; Shigeru Shimamura; Yoshihiro Takaki; Yohey Suzuki; Shun-ichi Murakami; Tamaki Watanabe; So Fujiyoshi; Sayaka Mino; Tomoo Sawabe; Takahiro Maeda; Hiroko Makita; Suguru Nemoto; Shin-Ichiro Nishimura; Hiromi Watanabe; Tomo-O Watsuji; Ken Takai
ISME JOURNAL, 8, 1, 40, 51, Jan. 2014, [Peer-reviewed]
English, Scientific journal

Physiologic and metagenomic attributes of the rhodoliths forming the largest CaCO3 bed in the South Atlantic Ocean
Giselle S. Cavalcanti; Gustavo B. Gregoracci; Eidy O. dos Santos; Cynthia B. Silveira; Pedro M. Meirelles; Leila Longo; Kazuyoshi Gotoh; Shota Nakamura; Tetsuya Iida; Tomoo Sawabe; Carlos E. Rezende; Ronaldo B. Francini-Filho; Rodrigo L. Moura; Gilberto M. Amado-Filho; Fabiano L. Thompson
ISME JOURNAL, 8, 1, 52, 62, Jan. 2014, [Peer-reviewed]
English, Scientific journal

Foreword
SAWABE TOMOO; SATOMI MASATAKA; YAMAZAKI KOJI
NSUGAF, 80, 6, 1002, 1002, The Japanese Society of Fisheries Science, 2014
Japanese

Draft genome sequence of marine flavobacterium Jejuia pallidilutea strain 11shimoA1 and pigmentation mutants
Naoki Takatani; Masato Nakanishi; Pedro Meirelles; Sayaka Mino; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Moriya Ohkuma; Masashi Hosokawa; Kazuo Miyashita; Fabiano L. Thompson; Ako Niwa; Toko Sawabe; Tomoo Sawabe
Genome Announcements, 2, 6, 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequences of marine flavobacterium Nonlabens strains NR17, NR24, NR27, NR32, NR33, and Ara13
Masato Nakanishi; Pedro Meirelles; Ryohei Suzuki; Naoki Takatani; Sayaka Mino; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Moriya Ohkuma; Masashi Hosokawa; Kazuo Miyashita; Fabiano L. Thompson; Ako Niwa; Toko Sawabe; Tomoo Sawabe
Genome Announcements, 2, 6, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequences of two Vibrionaceae species, Vibrio ponticus C121 and Photobacterium aphoticum C119, isolated as coral reef microbiota
Nurhidayu Al-saari; Pedro Milet Meirelles; Sayaka Mino; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Moriya Ohkuma; Fabiano L. Thompson; Bruno Gomez-Gil; Toko Sawabe; Tomoo Sawabe
Genome Announcements, 2, 5, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequences of marine flavobacterium Algibacter lectus strains SS8 and NR4
Naoki Takatani; Masato Nakanishi; Pedro Meirelles; Sayaka Mino; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Moriya Ohkuma; Masashi Hosokawa; Kazuo Miyashita; Fabiano L. Thompson; Ako Niwa; Toko Sawabe; Tomoo Sawabe
Genome Announcements, 2, 6, 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequences of Marinobacter similis A3d10T and Marinobacter salarius R9SW1T
Elena P. Ivanova; Hooi Jun Ng; Hayden K. Webb; Gao Feng; Kenshiro Oshima; Masahira Hattori; Moriya Ohkuma; Alexander F. Sergeev; Valery V. Mikhailov; Russell J. Crawford; Tomoo Sawabe
Genome Announcements, 2, 3, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Identification of a novel carotenoid, 2′-isopentenylsaproxanthin, by Jejuia pallidilutea strain 11shimoA1 and its increased production under alkaline condition
N. Takatani; K. Nishida; T. Sawabe; T. Maoka; K. Miyashita; M. Hosokawa
Applied Microbiology and Biotechnology, 98, 15, 6633, 6640, Springer Verlag, 2014, [Peer-reviewed]
English, Scientific journal

Metagenomic Analysis of Healthy and White Plague-Affected Mussismilia braziliensis Corals
Gizele D. Garcia; Gustavo B. Gregoracci; Eidy de O. Santos; Pedro M. Meirelles; Genivaldo G. Z. Silva; Rob Edwards; Tomoo Sawabe; Kazuyoshi Gotoh; Shota Nakamura; Tetsuya Iida; Rodrigo L. de Moura; Fabiano L. Thompson
MICROBIAL ECOLOGY, 65, 4, 1076, 1086, May 2013, [Peer-reviewed]
English, Scientific journal

Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific
Sayaka Mino; Hiroko Makita; Tomohiro Toki; Junichi Miyazaki; Shingo Kato; Hiromi Watanabe; Hiroyuki Imachi; Tomo-o Watsuji; Takuro Nunoura; Shigeaki Kojima; Tomoo Sawabe; Ken Takai; Satoshi Nakagawa
FRONTIERS IN MICROBIOLOGY, 4, 107, Apr. 2013, [Peer-reviewed]
English, Scientific journal

Microbial Communities Associated with Holothurians: Presence of Unique Bacteria in the Coelomic Fluid
Masaki Enomoto; Satoshi Nakagawa; Tomoo Sawabe
MICROBES AND ENVIRONMENTS, 27, 3, 300, 305, Sep. 2012, [Peer-reviewed]
English, Scientific journal

A simple method to collect digestive fluid for monitoring digestive enzyme activities and gut microbes of the abalone Haliotis discus discus
Kentaro Niwa; Hideaki Aono; Tomoo Sawabe
NIPPON SUISAN GAKKAISHI, 78, 5, 951, 957, Sep. 2012, [Peer-reviewed]
Japanese, Scientific journal

Vibrio jasicida sp nov., a member of the Harveyi clade, isolated from marine animals (packhorse lobster, abalone and Atlantic salmon)
Susumu Yoshizawa; Yasuhiro Tsuruya; Youhei Fukui; Tomoo Sawabe; Akira Yokota; Kazuhiro Kogure; Melissa Higgins; Jeremy Carson; Fabiano L. Thompson
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 62, 1864, 1870, Aug. 2012, [Peer-reviewed]
English, Scientific journal

Thermotomaculum hydrothermale gen. nov., sp nov., a novel heterotrophic thermophile within the phylum Acidobacteria from a deep-sea hydrothermal vent chimney in the Southern Okinawa Trough
Hiroshi Izumi; Takuro Nunoura; Masayuki Miyazaki; Sayaka Mino; Tomohiro Toki; Ken Takai; Yoshihiko Sako; Tomoo Sawabe; Satoshi Nakagawa
EXTREMOPHILES, 16, 2, 245, 253, Mar. 2012, [Peer-reviewed]
English, Scientific journal

A simple method to collect digestive fluid for monitoring digestive enzyme activities and gut microbes of the abalone Haliotis discus discus
Kentaro Niwa; Hideaki Aono; Tomoo Sawabe
Nippon Suisan Gakkaishi (Japanese Edition), 78, 5, 951, 957, The Japanese Society of Fisheries Science, 2012, [Peer-reviewed]
Japanese

The creation of probiotics for aquaculture
Haruo Sugita; Tomoo Sawabe; Mamoru Yoshimizu
Nippon Suisan Gakkaishi (Japanese Edition), 78, 4, 779, 779, The Japanese Society of Fisheries Science, 2012, [Peer-reviewed]
Japanese

蛍光標識テクノロジーを利用した食中毒細菌エコロジーの理解
澤辺桃子; 澤辺智雄
New Food Industry, 53, 8, 57, 66, 食品資材研究会, 2011, [Peer-reviewed]
Japanese

Rapid Detection of Staphylococcus aureus Using Fluorescence in situ Hybridization with Filter-Cultivation (FISHFC) Method
SHIMIZU Shigemasa; KUBOSAWA Yosuke; MATSUURA Misato; KAWAI Yuji; YAMAZAKI Koji
Japanese Journal of Food Microbiology, 28, 1, 29, 36, Japanese Society of Food Microbiology, 2011, [Peer-reviewed]
Japanese, Scientific journal, Conventional plating methods for enumerating Staphylococcus aureus are time-consuming and labor-intensive. Rapid methods for specific quantification are desirable. Fluorescence in situ hybridization with filter-cultivation (FISHFC) method has been already developed to enumerate viable specific microorganisms such as Listeria monocytogenes, Clostridium perfringens and Vibrio parahaemolyticus. The purpose of this study was to develop and estimate FISHFC method for S. aureus quantitative detection in food samples. Alexa Fluor^® 546-labeled oligonucleotide probe, STA68, was newly designed from the 16S rRNA gene sequences of S. aureus. STA68-conferred fluorescence was observed for S. aureus but not for any other organisms, suggesting that STA68 probe is highly specific for S. aureus. Results were achievable within 12 hours by FISHFC method, containing with 10 hours cultivation, as compared to more than 3 days required for confirmation of S. aureus by conventional plating methods. When inoculated to BPW and food samples, the numbers of viable counts determined by FISHFC method were not significantly different to those obtained by the conventional plating method (p>0.05). This result suggests that FISHFC method was preferable for the specific, rapid and accurate quantification of viable S. aureus in food.

Rapid Quantification of Escherichia coli as an Indicator of Food Contamination Using Fluorescence in situ Hybridization with Filter Cultivation (FISHFC)
Ryohei Aoi; Shigemasa Shimizu; Koji Yamazaki; Tomoo Sawabe; Yuji Kawai
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI, 58, 10, 483, 489, 2011, [Peer-reviewed]
Japanese

[Biodiversity and evolution of vibrios].
Sawabe T
Nihon saikingaku zasshi. Japanese journal of bacteriology, 65, 2, 333, 342, 2-4, Dec. 2010, [Peer-reviewed]
Japanese, ビブリオは，コレラ菌，腸炎ビブリオ及びビブリオ・バルニフィカスというヒト病原性種を含むことから，病原微生物学から環境微生物学までにおける幅広い学術分野でモデル微生物として活発に研究されている細菌群である。コレラ菌の発見から156年を経過した今でも，新種のビブリオが発見され続けており，自然界におけるビブリオの種多様性は驚くほど高い。1965年にM. Véonによって作られたビブリオ科（Vibrionaceae）は，6属，103種が包含される巨大な分類群となっている。ビブリオで新種が記載され続ける背景には，株の遺伝的多様性を精密に検出する指紋鑑定法や個体識別法が取り入れられたことにある。また，個体識別の過程で得られる多座位の遺伝子配列を解析することにより，ビブリオの進化の系譜や種分化機構の推定も試みられるようになってきた。さらに，ビブリオをモデルとしたゲノム情報に基づく分類規範の構築も始まっている。本稿ではビブリオの分類の歴史を紐解きながら，ビブリオの多様性と進化に関する最新の成果をまとめて紹介する。

Gene expression analysis of chemotactic responses against L-serine and fish mucus for Vibrio parahaemolyticus
澤辺桃子; 澤辺智雄
函館短期大学紀要, 38, 38, 13, 18, 函館短期大学, 2010, [Peer-reviewed]
Japanese

Environmental determinants correlated to Vibrio harveyi-mediated death of marine gastropods
Youhei Fukui; Sei-Ichi Saitoh; Tomoo Sawabe
ENVIRONMENTAL MICROBIOLOGY, 12, 1, 124, 133, Jan. 2010, [Peer-reviewed]
English, Scientific journal

細菌の進化の系譜をたどる新手法：多遺伝子座配列解析（MLSA）－見えてきた地球史とダイナミックにリンクするビブリオの進化
澤辺 智雄
化学と生物, 47, 1, 4, 6, 公益社団法人 日本農芸化学会, 2009, [Peer-reviewed]
Japanese

Enumeration of Pseudomonas aeruginosa by fluorescence in situ hybridization following cultivation (FISHFC) method
澤辺桃子; 大坪雅史; 澤辺智雄
The Bulletin of Hakodate Junior College, 35, 35, 51, 56, 函館短期大学, 2009, [Peer-reviewed]
Japanese

Diversity of Digestive Enzymes and Biochemical Properties of Major Polysaccharide Degrading Enzyme of Haliotis discus discus
NIWA Kentaro; AONO Hideaki; SAWABE Tomoo
Aquaculture Science, 57, 4, 557, 565, Japanese Society for Aquaculture Science, 2009, [Peer-reviewed]
Japanese, In order to elucidate the nutritional physiology on Kuro abalone Haliotis discus discus, activities of digestive enzymes were measured, and the three major polysaccharide degrading enzymes were purified and characterized.
Lipase, protease and polysaccharide degrading enzyme activities were detected from both digestive fluid and homogenate of digestive diverticulum of the abalone. The enzyme activities of alginate lyase, cellulase and mannanase were rather higher than the other enzyme activities. An alginate lyase, a cellulase and a mannanase were electrophoretically purified. MW of there purified enzymes were 27.5, 64.6, and 38.1 kDa, optimal temperatures were 40, 40 and 55°C, and optimal pH were 7.0, 5.0 and 5.0, respectively.

Poly(ethylene terephthalate) Polymer Surfaces as a Substrate for Bacterial Attachment and Biofilm Formation
Hayden K. Webb; Russell J. Crawford; Tom Sawabe; Elena P. Ivanova
MICROBES AND ENVIRONMENTS, 24, 1, 39, 42, 2009, [Peer-reviewed]
English, Scientific journal

Multi-Probe-Fluorescence in situ Hybridization for the Rapid Enumeration of Viable Vibrio parahaemolyticus
Tomoo Sawabe; Ai Yoshizawa; Yuko Kawanishi; Eriko Komatsu-Takeda; Satoshi Nakagawa; Toko Sawabe; Masashi Ootubo; Masataka Satomi; Yutaka Yano; Koji Yamazaki
MICROBES AND ENVIRONMENTS, 24, 3, 259, 264, 2009, [Peer-reviewed]
English, Scientific journal

Mutation is the Main Driving Force in the Diversification of the Vibrio splendidus Clade
Tomoo Sawabe; Shinichi Koizumi; Youhei Fukui; Satoshi Nakagawa; Elena P. Ivanova; Kumiko Kita-Tsukamoto; Kazuhiro Kogure; Fabiano L. Thompson
MICROBES AND ENVIRONMENTS, 24, 4, 281, 285, 2009, [Peer-reviewed]
English, Scientific journal

Vibrio porteresiae sp nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka)
N. Rameshkumar; Youhei Fukui; Tomoo Sawabe; Sudha Nair
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 58, 1608, 1615, Jul. 2008, [Peer-reviewed]
English, Scientific journal

自然界におけるビブリオ－その多様性、進化、生態
澤辺 智雄
化学療法の領域, 24, 6, 24, 32, (株)医薬ジャーナル社, 2008, [Peer-reviewed]
Japanese

集団遺伝学的方法論を取り入れたビブリオ科細菌の再分類
澤辺 智雄
IFO Res. Commun., 22, 17, 26, 2008, [Peer-reviewed]
Japanese, Scientific journal

Rapid detection of Vibrio harveyi in seawater by real-time PCR
Youhei Fukui; Tomoo Sawabe
MICROBES AND ENVIRONMENTS, 23, 2, 172, 176, 2008, [Peer-reviewed]
English, Scientific journal

『海洋微生物と共生-サンゴ礁・海底熱水孔の生き物たち-』, 石田祐三郎著, 成山堂書店, ベルソーブックス031, ISBN978-4-425-85291-8, 1,680円
澤辺 智雄
日本微生物生態学会誌, 23, 1, 28, 28, 日本微生物生態学会, 2008
Japanese

Inferring the evolutionary history of vibrios by means of multilocus sequence analysis
Tomoo Sawabe; Kumiko Kita-Tsukamoto; Fabiano L. Thompson
JOURNAL OF BACTERIOLOGY, 189, 21, 7932, 7936, Nov. 2007, [Peer-reviewed]
English, Scientific journal

Multilocus sequence analysis reveals that Vibrio harveyi and V-campbellii are distinct species
Fabiano L. Thompson; Bruno Gomez-Gil; Ana Teresa Ribeiro Vasconcelos; Tomoo Sawabe
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73, 13, 4279, 4285, Jul. 2007, [Peer-reviewed]
English, Scientific journal

Vibrio comitans sp nov., Vibrio rarus sp nov and Vibrio inusitatus sp nov., from the gut of the abalones Haliotis discus discus, H-gigantea, H-madaka and H-rufescens
Tomoo Sawabe; Yusuke Fujimura; Kentaro Niwa; Hideaki Aono
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 57, 916, 922, May 2007, [Peer-reviewed]
English, Scientific journal

Monitoring of fish-pathogenic vibrios
Tomoo Sawabe; Youhei Fukui
NIPPON SUISAN GAKKAISHI, 73, 2, 310, 312, Mar. 2007, [Peer-reviewed]
Japanese, Scientific journal

Mass mortality of Japanese abalone Haliotis discus hannai caused by Vibrio harveyi infection
Tomoo Sawabe; Sahoko Inoue; Youhei Fukui; Kaoru Yoshie; Yutaka Nishihara; Hiroki Miura
MICROBES AND ENVIRONMENTS, 22, 3, 300, 308, 2007, [Peer-reviewed]
English, Scientific journal

Improved one-step colony PCR detection of Vibrio harveyi
Youhei Fukui; Tomoo Sawabe
MICROBES AND ENVIRONMENTS, 22, 1, 1, 10, 2007, [Peer-reviewed]
English, Scientific journal

Simple conjugation and outgrowth procedures for tagging vibrios with GFP, and factors affecting the stable expression of the gfp tag
T. Sawabe; Y. Fukui; E. V. Stabb
LETTERS IN APPLIED MICROBIOLOGY, 43, 5, 514, 522, Nov. 2006, [Peer-reviewed]
English, Scientific journal

Vibrio2005: The First International Conference on the Biology of Vibrios
Fabiano L. Thompson; Karl E. Klose; Brian Austin; Yan Boucher; Peter Dawyndt; Dirk Gevers; Shah M. Faruque; Bruno Gomez-Gil; John F. Heidelberg; Tetsuya Iida; Didier Mazel; Diane McDougald; G. Balakrish Nair; Frans Ollevier; John H. Paul III; Martin Polz; Tomoo Sawabe; Ana C. P. Vicente; Rita R. Colwell; Jean Swings
Journal of Bacteriology, 188, 13, 4592, 4596, Jul. 2006, [Peer-reviewed]
English, International conference proceedings

Effect of iron on resuscitation of Tenacibaculum sp., the causative bacterium of spotting disease of short-spined sea urchin Strongylocentrotus intermedius, from the viable but non-culturable (VBNC) state
T Yamase; T Sawabe; K Kuma; K Tajima
FISH PATHOLOGY, 41, 1, 1, 6, Mar. 2006, [Peer-reviewed]
Japanese, Scientific journal

Adhesion of Tenacibaculum sp to short-spined sea urchin Strongylocentroutus intermedius and control of spotting disease by inhibiting adhesion using carbohydrate
R Taniguchi; T Sawabe; K Tajima
FISH PATHOLOGY, 41, 1, 13, 17, Mar. 2006, [Peer-reviewed]
English, Scientific journal

Effect of iron on resuscitation of Tenacibaculum sp., the causative bacterium of spotting disease of short-spined sea urchin Strongylocentrotus intermedius, from the viable but non-culturable (VBNC) state
Tomohisa Yamase; Tomoo Sawabe; Kensi Kuma; Kenichi Tajima
Fish Pathology, 41, 1, 1, 6, Mar. 2006, [Peer-reviewed]
Japanese, Scientific journal

Thalassomonas loyana sp nov, a causative agent of the white plague-like disease of corals on the Eilat coral reef
FL Thompson; Y Barash; T Sawabe; G Sharon; J Swings; E Rosenberg
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 56, 365, 368, Feb. 2006, [Peer-reviewed]
English, Scientific journal

Enhanced expression of active recombinant alginate lyase AlyPEEC cloned from a marine bacteriu Pseudoalteromonas elyakovii in Escherichia coli by calcium compounds
Sawabe, T; Takahasi; H. Saeki, H; Niwa, K; Aono, H
Enzyme and Microbial Technology, 40, 285, 291, 2006, [Peer-reviewed]
English, Scientific journal

PCR-RFLP analysis for identification of Aeromonas isolates collected from diseased fish and aquatic animals
MM Rahman; T Somsiri; R Tanaka; T Sawabe; K Tajima
FISH PATHOLOGY, 40, 4, 151, 159, Dec. 2005, [Peer-reviewed]
English, Scientific journal

Effect of Manipulation of Dietary Rotifer Bacterial Flora on the Intestinal Bacterial Flora of Japanese Flounder
SHIMIZU Tomoko; SHINOZAKI Daisuke; KASAI Hisae; SAWABE Tomoo; WATANABE Kenichi; YOSHIMIZU Mamoru
Aquaculture Science, 53, 3, 275, 278, Japanese Society for Aquaculture Science, 20 Sep. 2005
Japanese, Effect of manipulation of dietary rotifer Brachionus plicatilis bacterial flora on the intestinal bacterial flora of Japanese flounder Paralichthys olivaceus larvae was investigated. Manipulation was done by disinfecting rotifer eggs to reduce viable bacterial counts and then by inoculating hatched rotifers with non-sucrose fermenting Vibrio splendidus strain V-15 to make the bacterium dominant in the rotifers. Flounder larvae were fed the manipulated rotifers and intestinal bacterial flora of the fish was monitored. The dominant intestinal bacterial flora of the flounder became Vibrio spp. These results indicate that intestinal bacterial flora of flounder can be artificially controlled by manipulating bacterial flora in diets.

Presence of Ecophysiologically Diverse Populations within Cobetia marina Strains Isolated from Marine Invertebrate, Algae and the Environments
Elena P. Ivanova; Richard Christen; Tomoo Sawabe; Yulia V. Alexeeva; Anatoly M. Lysenko; Victor P. Chelomin; Valery V. Mikhailov
Microbes and Environments, 20, 4, 200, 207, 2005, [Peer-reviewed]
English, Scientific journal

Characterization of fish myofibrillar protein by conjugation with alginate oligosaccharide prepared using genetic recombinant alginate lyase
R Sato; T Sawabe; H Saeki
JOURNAL OF FOOD SCIENCE, 70, 1, C58, C62, Jan. 2005, [Peer-reviewed]
English, Scientific journal

Biodiversity and in situ abundance of gut microflora of abalone (Haliotis discus hannai) determined by culture-independent techniques
R Tanaka; M Ootsubo; T Sawabe; Y Ezura; K Tajima
AQUACULTURE, 241, 1-4, 453, 463, Nov. 2004, [Peer-reviewed]
English, Scientific journal

Molecular phylogeny of Neocalanus copepods in the subarctic Pacific Ocean, with notes on non-geographical genetic variations for Neocalanus cristatus
Marina Taniguchi; Toshinori Kanehisa; Tomoo Sawabe; Richard Christen; Tsutomu Ikeda
Journal of Plankton Research, 26, 10, 1249, 1255, Oct. 2004, [Peer-reviewed]
English, Scientific journal

Vibrio neonatus sp nov and Vibrio ezurae sp nov isolated from the Gut of Japanese Abalones
T Sawabe; K Hayashi; J Moriwaki; Y Fukui; FL Thompson; J Swings; R Christen
SYSTEMATIC AND APPLIED MICROBIOLOGY, 27, 5, 527, 534, Sep. 2004, [Peer-reviewed]
English, Scientific journal

Shewanella affinis sp nov., isolated from marine invertebrates
EP Ivanova; OI Nedashkovskaya; T Sawabe; NV Zhukova; GM Frolova; DV Nicolau; VV Mikhailov; JP Bowman
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 54, 1089, 1093, Jul. 2004, [Peer-reviewed]
English, Scientific journal

Vibrio gallicus sp nov., isolated from the gut of the French abalone Haliotis tuberculata
T Sawabe; K Hayashi; J Moriwaki; FL Thompson; J Swings; P Potin; R Christen; Y Ezura
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 54, 843, 846, May 2004, [Peer-reviewed]
English, Scientific journal

Sulfitobacter delicatus sp nov and Sulfitobacter dubius sp nov., respectively from a starfish (Stellaster equestris) and sea grass (Zostera marina)
EP Ivanova; NM Gorshkova; T Sawabe; NV Zhukova; K Hayashi; VV Kurilenko; Y Alexeeva; Buljan, V; DV Nicolau; VV Mikhailov; R Christen
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 54, 475, 480, Mar. 2004, [Peer-reviewed]
English, Scientific journal

Stereoisomers of marine bacterial phosphatidylglycerols
Hironori Fujishima; Toshiko Gamano; Yukako Taoka; Tomoo Sawabe; Yutaka Itabashi
Nippon Suisan Gakkaishi (Japanese Edition), 70, 2, 200, 202, Mar. 2004, [Peer-reviewed]
Japanese, Scientific journal

Vibrio superstes sp nov., isolated from the gut of Australian abalones Haliotis laevigata and Haliotis rubra
K Hayashi; J Moriwaki; T Sawabe; FL Thompson; J Swings; N Gudkovs; R Christen; Y Ezura
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 53, 1813, 1817, Nov. 2003, [Peer-reviewed]
English, Scientific journal

Gut microflora of abalone Haliotis discus hannai in culture changes coincident with a change in diet
R Tanaka; Sugimura, I; T Sawabe; M Yoshimizu; Y Ezura
FISHERIES SCIENCE, 69, 5, 951, 958, Oct. 2003, [Peer-reviewed]
English, Scientific journal

Stability and emulsion-forming ability of water-soluble fish myofibrillar protein prepared by conjugation with alginate oligosaccharide
R Sato; S Katayama; T Sawabe; H Saeki
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 51, 15, 4376, 4381, Jul. 2003, [Peer-reviewed]
English, Scientific journal

Occurrence and diversity of mesophilic Shewanella strains isolated from the North-West Pacific Ocean
EP Ivanova; T Sawabe; NV Zhukova; NM Gorshkova; OI Nedashkovskaya; K Hayashi; GM Frolova; AF Sergeev; KG Pavel; VV Mikhailov; DV Nicolau
SYSTEMATIC AND APPLIED MICROBIOLOGY, 26, 2, 293, 301, Jun. 2003, [Peer-reviewed]
English, Scientific journal

Acetic acid production of Vibrio halioticoli from alginate: a possible role for establishment of abalone-V-halioticoli association
T Sawabe; N Setoguchi; S Inoue; R Tanaka; M Ootsubo; M Yoshimizu; Y Ezura
AQUACULTURE, 219, 1-4, 671, 679, Apr. 2003, [Peer-reviewed]
English, Scientific journal

Shewanella fidelis sp nov., isolated from sediments and sea water
EP Ivanova; T Sawabe; K Hayashi; NM Gorshkova; NV Zhukova; OI Nedashkovskaya; VV Mikhailov; DV Nicolau; R Christen
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 53, 577, 582, Mar. 2003, [Peer-reviewed]
English, Scientific journal

Seven-hour fluorescence in situ hybridization technique for enumeration of Enterobacteriaceae in food and environmental water sample
M Ootsubo; T Shimizu; R Tanaka; T Sawabe; K Tajima; Y Ezura
JOURNAL OF APPLIED MICROBIOLOGY, 95, 6, 1182, 1190, 2003, [Peer-reviewed]
English, Scientific journal

Pseudomonas extremorientalis sp nov., isolated from a drinking water reservoir
EP Ivanova; NM Gorshkova; T Sawabe; K Hayashi; NI Kalinovskaya; AM Lysenko; NV Zhukova; DV Nicolau; TA Kuznetsova; VV Mikhailov; R Christen
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 52, 2113, 2120, Nov. 2002, [Peer-reviewed]
English, Scientific journal

Pseudoalteromonas translucida sp nov and Pseudoalteromonas paragorgicola sp nov., and emended description of the genus
EP Ivanova; T Sawabe; AM Lysenko; NM Gorshkova; K Hayashi; NV Zhukova; DV Nicolau; R Christen; VV Mikhailov
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 52, 1759, 1766, Sep. 2002, [Peer-reviewed]
English, Scientific journal

Fluorescent amplified fragment length polymorphism and repetitive extragenic palindrome-PCR fingerprinting reveal host-specific genetic diversity of Vibrio halioticoli-like strains isolated from the gut of Japanese abalone
T Sawabe; FL Thompson; J Heyrman; M Cnockaert; K Hayashi; R Tanaka; M Yoshimizu; B Hoste; J Swings; Y Ezura
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68, 8, 4140, 4144, Aug. 2002, [Peer-reviewed]
English, Scientific journal

Development of 16S rRNA targeted PCR for the identification of Vibrio spp., the causative bacteria of the disease in cultured sea urchin Strongylocentrotus intermedius occurring at low water temperatures
Y Sogabe; K Tajima; R Tanaka; T Sawabe; Y Ezura
NIPPON SUISAN GAKKAISHI, 68, 2, 201, 206, Mar. 2002, [Peer-reviewed]
Japanese, Scientific journal

Two species of culturable bacteria associated with degradation of brown algae Fucus evanescens
EP Ivanova; IY Bakunina; T Sawabe; K Hayashi; YV Alexeeva; NV Zhukova; DV Nicolau; TN Zvaygintseva; VV Mikhailov
MICROBIAL ECOLOGY, 43, 2, 242, 249, Mar. 2002, [Peer-reviewed]
English, Scientific journal

Identification of Vibrio halioticoli by colony hybridization with non-radioisotope labeled genomic DNA probe
R Tanaka; M Ootsubo; T Sawabe; K Tajima; J Vandenberghe; Y Ezura
FISHERIES SCIENCE, 68, 1, 227, 229, Feb. 2002, [Peer-reviewed]
English, Scientific journal

Pseudoalteromonas issachenkonii sp nov., a bacterium that degrades the thallus of the brown alga Fucus evanescens
EP Ivanova; T Sawabe; YV Alexeeva; AM Lysenko; NM Gorshkova; K Hayashi; NV Zukova; R Christen; VV Mikhailov
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 52, 229, 234, Jan. 2002, [Peer-reviewed]
English, Scientific journal

Pseudoalteromonas ruthenica sp nov., isolated from marine invertebrates
EP Ivanova; T Sawabe; AM Lysenko; NM Gorshkova; Svetashev, VI; DV Nicolau; N Yumoto; T Taguchi; S Yoshikawa; R Christen; VV Mikhailov
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 52, 235, 240, Jan. 2002, [Peer-reviewed]
English, Scientific journal

Pseudoalteromonas maricaloris sp nov., isolated from an Australian sponge, and reclassification of [Pseudoalteromonas aurantia] NCIMB 2033 as Pseudoalteromonas flavipulchra sp nov.
EP Ivanova; LS Shevchenko; TL Sawabe; AM Lysenko; Svetashev, VI; NM Gorshkova; M Satomi; R Christen; VV Mikhailov
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 52, 263, 271, Jan. 2002, [Peer-reviewed]
English, Scientific journal

Oligonucleotide probe for detecting Enterobacteriaceae by in situ hybridization
M Ootsubo; T Shimizu; R Tanaka; T Sawabe; K Tajima; M Yoshimizu; Y Ezura; T Ezaki; H Oyaizu
JOURNAL OF APPLIED MICROBIOLOGY, 93, 1, 60, 68, 2002, [Peer-reviewed]
English, Scientific journal

Cloning, sequence analysis and expression of Pseudoalteromonas elyakovii IAM 14594 gene (alyPEEC) encoding the extracellular alginate lyase
T Sawabe; H Takahashi; Y Ezura; P Gacesa
CARBOHYDRATE RESEARCH, 335, 1, 11, 21, Sep. 2001, [Peer-reviewed]
English, Scientific journal

Hydrophobic cluster analysis and classification of sixteen bacterial alginate lyases
J Kraiwattanapong; T Ooi; S Kinoshita; Sugimura, I; T Sawabe; Y Ezura
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 16, 3, 219, 224, Apr. 2000, [Peer-reviewed]
English, Scientific journal

Isolation of Pseudoalteromonas elyakovii strains from spot-wounded fronds of Laminaria japonica
T Sawabe; M Narita; R Tanaka; M Onji; K Tajima; Y Ezura
NIPPON SUISAN GAKKAISHI, 66, 2, 249, 254, Mar. 2000, [Peer-reviewed]
Japanese, Scientific journal

Characteristics of virus like growth suppression agents against phytoplankton obtained from seawater at the mouth of Funka Bay, Hokkaido, Japan
M Onji; T Sawabe; Y Ezura
FISHERIES SCIENCE, 66, 1, 38, 43, Feb. 2000, [Peer-reviewed]
English, Scientific journal

Variation of size-fractionated Fe concentrations and Fe(III) hydroxide solubilities during a spring phytoplankton bloom in Funka Bay (Japan)
Kenshi Kuma; Akira Katsumoto; Naonobu Shiga; Tomoo Sawabe; Katsuhiko Matsunaga
Marine Chemistry, 71, 1-2, 111, 123, 2000, [Peer-reviewed]
English, Scientific journal

Preparation of neoglycoprotein from carp myofibrillar protein and alginate oligosaccharide: Improved solubility in low ionic strength medium
R Sato; T Sawabe; H Kishimura; K Hayashi; H Saeki
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 48, 1, 17, 21, Jan. 2000, [Peer-reviewed]
English, Scientific journal

Growth suppression of the marine phytoplankton Alexandrium catenella and Tetraselmis sp caused by ultrafiltrated seawater collected from coastal area on the mouth of Funka Bay
M Onji; T Sawabe; Y Ezura
FISHERIES SCIENCE, 65, 5, 687, 693, Oct. 1999, [Peer-reviewed]
English, Scientific journal

Taxonomical and serological studies on the causative bacteria of the disease of sea urchin Strongylocentrotus intermedius occurring at low water temperatures
K Takeuchi; K Tajima; MM Iqbal; T Sawabe; Y Ezura
FISHERIES SCIENCE, 65, 2, 264, 268, Apr. 1999, [Peer-reviewed]
English, Scientific journal

Characterization of a facultatively psychrophilic bacterium, Vibrio rumoiensis sp. nov., that exhibits high catalase activity
Yumoto, I; H Iwata; T Sawabe; K Ueno; N Ichise; H Matsuyama; H Okuyama; K Kawasaki
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 65, 1, 67, 72, Jan. 1999, [Peer-reviewed]
English, Scientific journal

Phenotypic and genotypic identification of motile aeromonads isolated from fish with epizootic ulcerative syndrome in Southeast Asian countries
MM Iqbal; K Tajima; T Sawabe; K Nakano; Y Ezura
FISH PATHOLOGY, 33, 4, 255, 263, Oct. 1998, [Peer-reviewed]
English, Scientific journal

Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica
T Sawabe; H Makino; M Tatsumi; K Nakano; K Tajima; MM Iqbal; Yumoto, I; Y Ezura; R Christen
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 48, 769, 774, Jul. 1998, [Peer-reviewed]
English, Scientific journal

Intracellular alginate-oligosaccharide degrading enzyme activity that is incapable of degrading intact sodium alginate from a marine bacterium Alteromonas sp.
T Sawabe; C Sawada; E Suzuki; Y Ezura
FISHERIES SCIENCE, 64, 2, 320, 324, Apr. 1998, [Peer-reviewed]
English, Scientific journal

Bacillus horti sp. nov., a new Gram-negative alkaliphilic Bacillus
Yumoto, I; K Yamazaki; T Sawabe; K Nakano; K Kawasaki; Y Ezura; H Shinano
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 48, 565, 571, Apr. 1998, [Peer-reviewed]
English, Scientific journal

Plant regeneration from protoplasts of Laminaria japonica Areschoug (Laminariales, Phaeophyceae) in a continuous-flow culture system
T Sawabe; Y Ezura; H Yamamoto
PLANT CELL REPORTS, 17, 2, 109, 112, Dec. 1997, [Peer-reviewed]
English, Scientific journal

Detection and identification of infectious hematopoietic necrosis virus (IHNV) by reverse transcription (RT)-polymerase chain reaction (PCR)
T Yoshinaka; M Yoshimizu; T Sawabe; Y Ezura
FISHERIES SCIENCE, 63, 4, 592, 595, Aug. 1997, [Peer-reviewed]
English, Scientific journal

リボソーマルタンパク質遺伝子の塩基配列に基づくビブリオの分子系統
志水智美; 田仲真実; 美野さやか; 澤辺智雄, 日本水産学会大会講演要旨集, 2020, 2020

沖縄トラフ深海底熱水活動域由来の新規好熱性Campylobacteria
深澤操; 美野さやか; 澤辺智雄; 中川聡; 高井研, 日本水産学会北海道支部大会講演要旨集, 2020 (CD-ROM), 2020

水素生成マリンビブリオのギ酸水素リアーゼ(FHL)複合体遺伝子群の構造比較
西川 紗代; 田仲 真実; 美野 さやか; 澤辺 智雄; 小椋 義俊; 林 哲也, 日本細菌学雑誌, 75, 1, 80, 80, Jan. 2020
日本細菌学会, Japanese

Vibriosis in cultured marine fishes: a review
Nurliyana Mohamad; Mohammad Noor Azmai Amal; Ina Salwany Md Yasin; Mohd Zamri Saad; Nurrul Shaqinah Nasruddin; Nurhidayu Al-saari; Sayaka Mino; Tomoo Sawabe, Aquaculture, 512, 15 Oct. 2019
Elsevier B.V., English, Book review

損傷菌 水産における損傷菌
澤辺 智雄; 里見 正隆, 日本防菌防黴学会誌, 47, 10, 423, 429, Oct. 2019
日本防菌防黴学会, Japanese

ニッケル輸送体遺伝子型は海洋ビブリオの水素産生能と相関する(Type of nickel transporter genes correlate with ability of hydrogen production in marine vibrios)
松村 佑太; 美野 さやか; 丸山 史人; 小椋 義俊; 林 哲也; 黒川 顕; 澤辺 智雄, 日本細菌学雑誌, 73, 1, 72, 72, Feb. 2018
日本細菌学会, English

Occurrence and Prevention of Injured Bacterial Cells in Fisheries
Koji Yamazaki; Kantaro Takeuchi; Yohei Yamazaki; Sayaka Mino; Hisae Kasai; Toko Sawabe; Tomoo Sawabe; Masataka Satomi, JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI, 65, 4, 197, 204, 2018
Japanese, Book review

アルギン酸から水素を生成可能な新規ビブリオ属細菌の分類学的検討
田仲真実; 美野さやか; 土井秀高; 澤辺桃子; 澤辺智雄; 荒木利芳, マリンバイオテクノロジー学会大会講演要旨集, 19th, 89, 03 Jun. 2017
Japanese

RNA‐Seqで探る腸炎ビブリオのストレス応答
竹内勘太郎; 美野さやか; 澤辺智雄; 澤辺桃子; 里見正隆, 日本水産学会大会講演要旨集, 2017, 84, 26 Mar. 2017
Japanese

ナマコの消化管マイクロバイオーム:メタゲノム解析による成長格差の解明
山崎耀平; 美野さやか; 澤辺智雄, バイオサイエンスとインダストリー, 74, 6, 508‐509, 10 Nov. 2016
Japanese

マリンビブリオを生物触媒としたアルギン酸の水素変換
天田愛梨; 松村祐太; AL‐SAARI Nurhidayu; 美野さやか; 澤辺智雄; 澤辺桃子, 日本水産学会大会講演要旨集, 2016, 33, 08 Sep. 2016
Japanese

Vibrio tritonius AM2株のギ酸水素リアーゼ遺伝子群のトランスクリプトーム解析
水越草太; 松村祐太; AL‐SAARI Nurhidayu; 美野さやか; 澤辺智雄; 澤辺桃子, 日本水産学会大会講演要旨集, 2016, 33, 08 Sep. 2016
Japanese

16SrRNAタイピング法を用いた幼児用簡易プール水の細菌群集構造解析
澤辺 桃子; 須田 亙; 大島 健志朗; 服部 正平; 澤辺 智雄, 日本細菌学雑誌, 71, 1, 63, 63, Feb. 2016
日本細菌学会, Japanese

深海底熱水活動域に普遍的に生息する化学合成独立栄養細菌の生物地理学的特徴の解明
美野さやか; 中川聡; 牧田寛子; 工藤桃李; 宮崎淳一; 稲垣史生; 加藤真悟; 布浦拓郎; 井町寛之; 和辻智郎; 高井研; 澤辺智雄, 日本微生物生態学会大会(Web), 31st, 183 (WEB ONLY), 2016
Japanese

ビブリオ科細菌が有するCRISPR/Casシステムの特徴
遠藤 祥子; 美野 さやか; 澤辺 智雄, 日本生物工学会大会講演要旨集, 平成27年度, 201, 201, Sep. 2015
(公社)日本生物工学会, Japanese

水素生成マリンビブリオの網羅的発現解析
澤辺智雄; 水越草太; 美野さやか; 松村佑太; 澤辺桃子, マリンバイオテクノロジー学会大会講演要旨集, 17th, 96, 30 May 2015
Japanese

マナマコApostichopus japonicusの個体間微生物叢比較
山崎耀平; 美野さやか; 澤辺智雄; 須田亙; 大島健志朗; 服部正平; MEIRELLES P.M; THOMPSON F.L, マリンバイオテクノロジー学会大会講演要旨集, 17th, 99, 30 May 2015
Japanese

目指せ!細菌学の星☆2015 深海底熱水活動域における固有甲殻類血清の性状解析(We'll be the stars of bacteriology! 2015 Hemagglutination activity in the serum of deep-sea vent endemic crab)
藤吉 奏; 福島 大介; 和辻 智郎; 美野 さやか; 澤辺 智雄; 澤山 茂樹; 高井 研; 中川 聡, 日本細菌学雑誌, 70, 1, 113, 113, Feb. 2015
日本細菌学会, English

海洋細菌Jejuia pallidilutea 11shimoA1株の新規カロテノイド生合成系解析
高谷直己; 澤辺智雄; 宮下和夫; 細川雅史, マリンバイオテクノロジー学会大会講演要旨集, 17th, 2015

深海底熱水活動域に生息する化学合成独立栄養細菌の集団遺伝学:遺伝的多様性と分布パターンの解明
美野さやか; 中川聡; 中川聡; 牧田寛子; 宮崎淳一; 加藤真悟; 和辻智郎; 井町寛之; 布浦拓郎; STEFAN Sievert; ANNE Godfroy; 高井研; 澤辺智雄, 日本微生物生態学会大会(Web), 30th, OA‐38 (WEB ONLY), 2015
Japanese

深海底熱水活動域に生息する巻貝類とその共生細菌の群集遺伝学的解析
村上竣一; 益崎庸介; 美野さやか; 澤辺智雄; 宮崎淳一; CHEN Chong; ROGERS Alex; COPLEY Jonathan; 高井研; 中川聡, 日本土壌微生物学会講演要旨集, 2014, 129, 129, 16 Oct. 2014
日本微生物生態学会, Japanese

海洋細菌が有するカロテノイド合成系の体系的理解:フラボバクテリアにおけるmyxol合成系の特徴
中西誠人; 高谷直己; 高峰; 宮下和夫; 細川雅史; 大熊盛也; 大島健志朗; 服部正平; 澤辺智雄, 日本水産学会大会講演要旨集, 2014, 34, 19 Sep. 2014
Japanese

The famlily vibrionaceae
Bruno Gomez-Gil; Cristinane C. Thompson; Yuta Matsumura; Toko Sawabe; Tetsuya Iida; Richard Christen; Fabiano Thompson; Tomoo Sawabe, The Prokaryotes: Gammaproteobacteria, 9783642389221, 659, 747, 01 Jul. 2014
Springer-Verlag Berlin Heidelberg, English

生産・流通・加工工程における体系的な危害要因の特性解明とリスク低減技術の開発〈かび毒・病原微生物〉第4章 水産物における病原微生物のリスク低減技術の開発(1)沿岸漁場および水揚げ場における腸炎ビブリオの動態解明
澤辺智雄; 澤辺桃子, 農林水産省農林水産技術会議事務局研究成果, 522, 156, 160, 31 Mar. 2014
Japanese

O35-04 浅海性無脊椎動物の体腔液に共存する微生物の群集構造解析(口頭発表)
砂田 高志; 中川 聡; 澤辺 智雄, 日本微生物生態学会講演要旨集, 2014, 128, 128, 2014
日本微生物生態学会, Japanese

1P-144 Bioconversion of formate to hydrogen using marine vibrio and the optimum condition
Amada Eri; Sawabe Tomoo, 日本生物工学会大会講演要旨集, 66, 54, 54, 2014
日本生物工学会, Japanese

1P-145 Metabolic engineering of Vibrio halioticoli for increase of biofuel productivity from starch-based marine biomass
Sugitani Mai; Sawabe Tomoo, 日本生物工学会大会講演要旨集, 66, 54, 54, 2014
日本生物工学会, Japanese

2P-026 Global gene expression of marine vibrio
Mizukoshi Sota; Maruyama Fumito; Ogura Yoshitoshi; Hayashi Tetsuya; Kurokawa Ken; Sawabe Tomoo, 日本生物工学会大会講演要旨集, 66, 113, 113, 2014
日本生物工学会, Japanese

深海底熱水活動域に生息する無脊椎動物の血リンパ中レクチンの探索と性状解析
藤吉奏; 和辻智郎; 美野さやか; 澤辺智雄; 澤山茂樹; 高井研; 中川聡; 中川聡, 日本土壌微生物学会講演要旨集, 2014, 2014

Sulfurovum aggregans sp. nov., A hydrogenoxidizing, Thiosulfate-reducing chemolithoautotroph within the Epsilonproteobacteria isolated from a deep-sea hydrothermal vent chimney, And an emended description of the genus Sulfurovum
Sayaka Mino; Hideaki Kudo; Takayuki Arai; Tomoo Sawabe; Ken Takai; Satoshi Nakagawa; Satoshi Nakagawa; Satoshi Nakagawa, International Journal of Systematic and Evolutionary Microbiology, 64, 3195, 3201, 01 Jan. 2014

深海底熱水活動域に生息する化学合成微生物の分布様式と集団構造の解明
美野さやか; 中川聡; 牧田寛子; 宮崎淳一; 和辻智郎; 渡部裕美; 西澤学; 中村謙太郎; 布浦拓郎; 井町寛之; 山本正浩; 高井研; 加藤真悟; 小島茂明; 島伸和; 石橋純一郎; 澤辺智雄, ブルーアース要旨集, 2014, 106, 2014
Japanese

海藻糖質の代謝過程におけるVibrio halioticoliの遺伝子発現解析
久我康太; 中川聡; 丸山史人; 小椋義俊; 林哲也; 黒川顕; 澤辺桃子; 澤辺智雄, 日本生物工学会大会講演要旨集, 65th, 134, 134, 25 Aug. 2013
日本生物工学会, Japanese

めざせ!細菌学の星 マリンビブリオの水素生産能の評価および実用化へのアプローチ(Young researchers workshop on bacteriology 2013 Characterization of hydrogen producing marine vibrios and the practical approach)
佐藤 一道; 澤辺 智雄, 日本細菌学雑誌, 68, 1, 103, 103, Feb. 2013
日本細菌学会, English

3S-Fa02 Bioenergy Production Using Marine Microbes
Sawabe Tomoo, 日本生物工学会大会講演要旨集, 65, 184, 184, 2013
日本生物工学会, English

3P-167 Development of hydrogen production system from seaweed carbohydrates using Vibrio tritonius AM2^T
Sato Kazumichi; Amada Eri; Nakagawa Satoshi; Sawabe Tomoo, 日本生物工学会大会講演要旨集, 65, 230, 230, 2013
日本生物工学会, Japanese

海洋細菌Jejuia pallidilutea A1株の新規カロテノイド合成とストレス応答
高谷直己; 西田健太郎; 澤辺智雄; 宮下和夫; 細川雅史, マリンバイオテクノロジー学会大会講演要旨集, 15th, 2013

深海底熱水活動域に棲息する大型無脊椎動物の血清中レクチンの探索と性状解析
藤吉奏; 中川聡; 美野さやか; 神藤彩加; 新井崇之; 澤辺智雄; 杉村誠; 和辻智郎; 山本正浩; 丸茂克美, ブルーアース要旨集, 2013, 2013

Vibrio halioticoliの代謝改変:Zymomonas mobilis由来のピルビン酸脱炭酸酵素およびアルコール脱水素酵素IIの発現
猪原悠太郎; 中川聡; 澤辺智雄, 日本生物工学会大会講演要旨集, 65th, 2013

深海底熱水活動域の固有甲殻類が有するレクチンの精製と性状解析
藤吉奏; 新井崇之; 美野さやか; 和辻智郎; 澤辺智雄; 中川聡, 日本糖質学会年会要旨集, 32nd, 2013

食品の安全を確保するための評価・管理技術の開発 第1章 有害微生物検出技術の開発 3 高感度蛍光マイクロコロニー法による食中毒細菌の複数同時検出・定量システムの開発
山崎浩司; 澤辺智雄, 農林水産省農林水産技術会議事務局研究成果, 491, 32, 37, Jan. 2013
Japanese, Technical report

Updating the Vibrio clades defined by multilocus sequence phylogeny: Proposal of eight new clades, and the description of Vibrio tritonius sp. nov.
Tomoo Sawabe; Yoshitoshi Ogura; Yuta Matsumura; Gao Feng; A. K.M. Rohul Amin; Sayaka Mino; Satoshi Nakagawa; Toko Sawabe; Ramesh Kumar; Yohei Fukui; Masataka Satomi; Ryoji Matsushima; Fabiano L. Thompson; Bruno Gomez-Gil; Richard Christen; Fumito Maruyama; Ken Kurokawa; Tetsuya Hayashi, Frontiers in Microbiology, 4, 414, 1, 14, 2013
Frontiers Research Foundation, English

PK-05 Characterization of serum lectins from a deep-sea hydrothermal vent invertebrate
Fujiyoshi So; Arai Takayuki; Kando Ayaka; Mino Sayaka; Watsuji Tomoo; Sugimura Makoto; Sawabe Tomoo; Marumo Katsumi; Nakagawa Satoshi, 日本微生物生態学会講演要旨集, 28, 171, 171, 2012
日本微生物生態学会, English

PK-04 Structural comparison of lipopolysaccharides isolated from deep-sea hydrothermal vent chemoautotrophs(PK Interaction and symbiosis,Poster session)
Kando Ayaka; Arai Takayuki; Fujiyoshi So; Mino Sayaka; Watsuji Tomoo; Sugimura Makoto; Sawabe Tomoo; Marumo Katsumi; Nakagawa Satoshi, 日本微生物生態学会講演要旨集, 28, 171, 171, 2012
日本微生物生態学会, English

PK-03 Structural characterization of LPS (lipopolysaccharide) from deep-sea vent symbiont and its free-living counterparts(PK Interaction and symbiosis,Poster session)
Arai Takayuki; Fujiyoshi So; Kando Ayaka; Hirose Tomohiro; Cruise participants YK09-13; Sawabe Tomoo; Nakagawa Satoshi, 日本微生物生態学会講演要旨集, 28, 171, 171, 2012
日本微生物生態学会, English

ビブリオは線毛タンパク質PilAを介して海産魚腸内受容体に接着する
松吉亮; 徳永遼平; 松永尚之; 澤辺智雄; 沖野望; 伊東信, 日本水産学会大会講演要旨集, 2012, 2012

深海底熱水活動域に固有の無脊椎動物が有する血清中レクチンの性状
藤吉奏; 新井崇之; 神藤彩加; 美野さやか; 和辻智郎; 杉村誠; 澤辺智雄; 丸茂克美; 中川聡, 日本糖質学会年会要旨集, 31st, 2012

I-4. Recent progress in the gut microbial research of benthic marine invertebrates
Tomoo Sawabe; Satoshi Nakagawa; Toko Sawabe, Nippon Suisan Gakkaishi (Japanese Edition), 78, 4, 783, 783, 2012
The Japanese Society of Fisheries Science, Japanese

PK-02 Population genetic analysis of deep-sea hydrothermal vent gastropod Alviniconcha and their bacterial endosymbionts(PK Interaction and symbiosis,Poster session)
Masuzaki Yosuke; Mino Sayaka; Watanabe Hiromi; Miyazaki Junichi; Nunoura Takuro; Kojima Shigeaki; Sawabe Tomoo; Nakagawa Satoshi, 日本微生物生態学会講演要旨集, 28, 170, 170, 2012
日本微生物生態学会, English

深海底熱水活動域に生息する化学合成微生物の遺伝学的多様性と群集構造の解明
美野さやか; 中川聡; 澤辺智雄; 宮崎淳一; 牧田寛子; 和辻智朗; 中村謙太郎; 加藤真悟; 小島茂明; 渡部裕美; 土岐知弘; 島伸和; 石橋純一郎; 布浦拓郎; 山本正浩; 高井研, ブルーアース要旨集, 2012, 185, 2012
Japanese

New frontier in Fisheries Sciences beyond the current microbial genome research
Yoshihiko Sako; Takashi Yoshida; Tomoo Sawabe, NIPPON SUISAN GAKKAISHI, 77, 2, 246, 246, Mar. 2011
Japanese, Others

Application of genome sequences for molecular epidemiological studies of pathogenic vibrios
Tomoo Sawabe, NIPPON SUISAN GAKKAISHI, 77, 2, 251, 251, Mar. 2011
Japanese, Others

Visualization of chemotactic behavior for Vibrio parahaemolyticus
澤辺 桃子; 坂牛 百合香; 澤辺 智雄, The Bulletin of Hakodate Junior College, 37, 41, 45, Mar. 2011
函館短期大学, Japanese

インド洋の深海底熱水活動域に棲息する細胞内共生微生物のゲノム解析
中川聡; 島村繁; 高木善弘; 高井研; 澤辺智雄, ブルーアース要旨集, 2011, 2011

PB-006 Vibrio tritoniusのアルコール脱水素酵素遺伝子の多様性(PB 生理/代謝/増殖,ポスター発表)
李 羨; 中川 聡; 丸山 史人; 小椋 義俊; 林 哲也; 黒川 顕; 澤辺 智雄, 日本微生物生態学会講演要旨集, 27, 85, 85, 2011
日本微生物生態学会, Japanese

腸炎ビブリオによるカキの汚染動態解析
坂牛百合香; 中川聡; 澤辺智雄; 大橋智志; 矢野豊; 里見正隆, 日本水産学会大会講演要旨集, 2011, 2011

Foreword
SAKO YOSHIHIKO; YOSHIDA TAKASHI; SAWABE TOMOO, NSUGAF, 77, 2, 2011
The Japanese Society of Fisheries Science, Japanese

PA-043 深海底熱水活動域から分離・培養された新規好熱性Acidobacteriaの性状解析(PA 極限環境,ポスター発表)
和泉 裕志; 布浦 拓郎; 宮崎 征行; 美野 さやか; 土岐 知弘; 高井 研; 左子 芳彦; 澤辺 智雄; 中川 聡, 日本微生物生態学会講演要旨集, 27, 76, 76, 2011
日本微生物生態学会, Japanese

ビブリオの多様性と進化
澤辺 智雄, 日本細菌学雑誌, 65, 2-3-4, 333, 342, Dec. 2010
日本細菌学会, Japanese

腸炎ビブリオの魚介類粘液に対する走性応答の可視化
澤辺 桃子; 坂牛 百合香; 清水 陽子; 澤辺 智雄, 日本食品衛生学会学術講演会講演要旨集, 100回, 136, 136, Sep. 2010
(公社)日本食品衛生学会, Japanese

Optimum condition for microcolony formation to detect starved Pseudomonas aeruginosa contaminated in mineral water by fluorescence in situ hybridization following cultivation (FISHFC)
澤辺 桃子; 大坪 雅史; 澤辺 智雄, The Bulletin of Hakodate Junior College, 36, 23, 28, Mar. 2010
函館短期大学, Japanese

P-55 Genome analysis of endosymbionts in a deep-sea hydrothermal field, Central Indian Ridge(Poster Session)
NAKAGAWA SATOSHI; SHIMAMURA SHIGERU; TAKAKI YOSHIHIRO; TAKAI KEN; SAWABE TOMOO; SCIENTISTS YK09-13 LEG2, 日本微生物生態学会講演要旨集, 26, 122, 122, 2010
日本微生物生態学会, Japanese

P-49 Taxonomic and physiological characterizations of novel bacteria isolated from deep-sea hydrothermal vent chimneys(Poster Session)
MORI NAOYA; NAKAGAWA SATOSHI; GODFROY ANNE; TAKAI KEN; SAWABE TOMOO, 日本微生物生態学会講演要旨集, 26, 119, 119, 2010
日本微生物生態学会, Japanese

P-69 Molecular phylogenetic characters of microbes detected in body fluid of shallow-sea invertebrates(Poster Session)
AIDA MASANORI; NAKAGAWA SATOSHI; SAWABE TOMOO, 日本微生物生態学会講演要旨集, 26, 129, 129, 2010
日本微生物生態学会, Japanese

海洋好熱細菌Nitratiruptor sp.由来アルギン酸リアーゼの性状解析
井上晶; 安楽萌; 中川聡; 澤辺智雄; 尾島孝男, 日本水産学会大会講演要旨集, 2010, 2010

GFP標識ビブリオ病原因菌Vibrio harveyiの糖脂質受容体への接着
清水耕平; 苣田慎一; 松永尚之; 座間宏太; 澤辺智雄; 沖野望; 伊東信, 日本水産学会大会講演要旨集, 2010, 2010

エゾアワビ肝膵臓におけるアルギン酸代謝に関する研究
木本雄太; 熊谷祐也; 澤辺智雄; 田中啓之; 井上晶; 尾島孝男, 日本水産学会大会講演要旨集, 2010, 2010

P-50 An approach to clarifying the population genetic structures of chemoautotrophic bacteria in deep-sea hydrothermal fields(Poster Session)
MINO SAYAKA; NAKAGAWA SATOSHI; MAKITA HIROKO; INAGAKI FUMIO; YAMAMOTO MASAHIRO; NONOURA TAKURO; NAKAMURA KOICHI; GODFROY ANNE; TAKAI KEN; SAWABE TOMOO, 日本微生物生態学会講演要旨集, 26, 119, 119, 2010
日本微生物生態学会, Japanese

培養併用蛍光in situハイブリダイゼーション法を用いた腸炎ビブリオの計数
川西 優子; 澤辺 桃子; 大坪 雅史; 澤辺 智雄, 日本食品衛生学会学術講演会講演要旨集, 98回, 83, 83, Oct. 2009
(公社)日本食品衛生学会, Japanese

GFP標識によるビブリオ属細菌と糖脂質受容体の接着の可視化
清水 耕平; 苣田 慎一; 松永 尚之; 座間 宏太; 澤辺 智雄; 沖野 望; 伊東 信, 日本生化学会大会プログラム・講演要旨集, 82回, 4P, 066, Sep. 2009
(公社)日本生化学会, Japanese

『海の微生物の利用-未知なる宝探し-』, 今田千秋著, 成山堂書店, ISBN978-4-425-88451-3, 1,680円
澤辺 智雄, 日本微生物生態学会誌, 24, 2, 77, 78, 2009
日本微生物生態学会, Japanese

Development of a new monitoring system for food-borne pathogens
OOTSUBO MASASHI; MASASHI OOTSUBO, NIPPON SUISAN GAKKAISHI, 74, 2, 253, 254, 15 Mar. 2008
The Japanese Society of Fisheries Science, Japanese

Shewanella属菌のOXA-型カルバペネマーゼ保有状況に関する検討
石井 良和; 嵯峨 知生; 山本 啓之; 野中 里佐; 山口 惠三; 神山 裕一; 澤辺 智雄; 里見 正隆, 日本細菌学雑誌, 63, 1, 106, 106, Feb. 2008
日本細菌学会, Japanese

11-220 Effect of warming trend of seawater on Vibrio harveyi infection for marine animals(Aquatic ecosystem)
Fukui Youhei; Sawabe Tomoo, 日本微生物生態学会講演要旨集, 24, 168, 168, 2008
日本微生物生態学会, Japanese

テラフォーミング : 環境微生物学の挑戦(学際シンポジウム報告)
澤辺 智雄; 宍戸 雅宏; 吉永 郁生, 日本微生物生態学会誌, 23, 2, 73, 74, 2008
日本微生物生態学会, Japanese

『食中毒のリスクと人間社会』, 清水潮著, 幸書房, B6判, 225頁, 1,890円, ISBN978-4782103142
澤辺 智雄, 日本微生物生態学会誌, 23, 2, 75, 75, 2008
日本微生物生態学会, Japanese

06-109 Diversity of microbes associated with a sea cucumber, Apostichopus japonicus Selenka(Microbial community analysis)
Enomoto Masaki; Sawabe Tomoo, 日本微生物生態学会講演要旨集, 24, 113, 113, 2008
日本微生物生態学会, Japanese

10-199 Rapid fluorescence in situ hybridization detection (FISH) of viable Campylobacter jejuni and Campylobacter coli(Environmental sanitation)
Sawabe Tomoo; Yoshizawa Ai; Sawabe Toko; Otsubo Masashi; Ito Takeshi, 日本微生物生態学会講演要旨集, 24, 158, 158, 2008
日本微生物生態学会, Japanese

Specific detection of Listeria spp. using fluorescent in situ hybridization in combination with filter cultivation (FISHFC)
Yamazaki, K; Fuchizawa, I; Kawai, Y; Sawabe, T; Ootsubo, M, Proceedings of the United States – Japan Cooperative Program in Natural Resources (UJNR) Food and Agriculture Panel 37th Annual Meeting, 60, 61, 2008
English, Introduction international proceedings

Real-time monitoring of microbes in aquatic environments and seafood
Tomoo Sawabe, NIPPON SUISAN GAKKAISHI, 73, 2, 290, 291, Mar. 2007
Japanese, Others

PB-30 加熱損傷したEnterobacteriaceaeの培養併用FISH法による迅速検出(モニタリング,ポスターセッションB,ポスター発表)
大坪 雅史; 剣持 美帆; 宮原 則行; 山崎 浩司; 澤辺 智雄; 高橋 信行; 小高 秀正; 伊藤 武, 日本微生物生態学会講演要旨集, 23, 129, 129, 2007
日本微生物生態学会, Japanese

PB-23 培養併用蛍光インサイチューハイブリダイゼーション法による腸炎ビブリオの検出(モニタリング,ポスターセッションB,ポスター発表)
澤辺 智雄; 吉澤 愛; 大坪 雅史, 日本微生物生態学会講演要旨集, 23, 126, 126, 2007
日本微生物生態学会, Japanese

PB-24 培養併用蛍光インサイチューハイブリダイゼーション法による緑膿菌の迅速検出(モニタリング,ポスターセッションB,ポスター発表)
吉澤 愛; 大坪 雅史; 澤辺 智雄, 日本微生物生態学会講演要旨集, 23, 126, 126, 2007
日本微生物生態学会, Japanese

南極舟底池および子鉢池における真核微生物群集
三宅絵理; 澤辺智雄; 大内真理子; 堀口健雄; 松崎雅広; 伊村智, 極域気水圏シンポジウムプログラム・講演要旨, 29th, 132, Nov. 2006
Japanese

PB-63 Development of quantitative real-time PCR for Vibrio harveyi(Aquatic ecosystem,Session B,(1) Poster Presentation)
Fukui Youhei; Sawabe Tomoo; Tajima Kenichi, 日本微生物生態学会講演要旨集, 22, 181, 181, 2006
日本微生物生態学会, Japanese

PB-64 Distribution of the abalone pathogenic Vibrio harveyi around the abalone farm(Aquatic ecosystem,Session B,(1) Poster Presentation)
Fukui Youhei; Sawabe Tomoo; Tajima Kenichi, 日本微生物生態学会講演要旨集, 22, 182, 182, 2006
日本微生物生態学会, Japanese

C-10 Evaluation of Fluorescence In Situ Hybridization Following Cultivation for Rapid Enumeration of Enterobacteriaceae in Various Foods.(FOOD HYGIENE,(2) Oral presentation)
Ootsubo Masashi; Kenmotsu Miho; MIyahara Noriyuki; Shimizu Takeshi; Sawabe Tomoo; Yamazaki Koji, 日本微生物生態学会講演要旨集, 21, 254, 254, 2005
日本微生物生態学会, Japanese

PA-42 Distribution of Vibrio cholerae and Vibrio parahaemolyticus virulence genes among Vibrionaceae(ENVIRONMENTAL HYGIENE,Session A,(1) Poster presentations)
Saito Yuki; Sawabe Tomoo; Tajima Kenichi, 日本微生物生態学会講演要旨集, 21, 88, 88, 2005
日本微生物生態学会, Japanese

PB-44 Molecular phylogeny of Vibrionaceae based on glyceraldehyde-3-phosphate dehydrogenase gene(CLASSIFICATION/PHYLOGENETIC ANALYSIS,Session B,(1) Poster presentations)
Sawabe Tomoo; Saito Yuki; Koizumi Shinichi; Tajima Kenichi, 日本微生物生態学会講演要旨集, 21, 164, 164, 2005
日本微生物生態学会, Japanese

Occurence of Unnatural Phospholipid in Marine Bacteria
Gamano Toshiko; Fujishima Hironori; Sawabe Tomoo; Itabashi Yutaka, Bulletin of Fisheries Sciences, Hokkaido University, 55, 1, 17, 21, Aug. 2004
Hokkaido University, Japanese

アワビ消化管から分離されたVibrio halioticoli類縁種の遺伝的多様性を反映する遺伝子
沢辺智雄; 森脇潤; 田島研一; 絵面良男, 日本水産学会大会講演要旨集, 2004, 2004

29-B-03 培養併用FISH法による食品および環境からの腸内細菌科細菌の迅速定量(食品衛生,一般講演)
大坪 雅史; 澤辺 智雄; 田島 研一, 日本微生物生態学会講演要旨集, 19, 136, 136, 2003
日本微生物生態学会, Japanese

アワビ消化管内から分離された新規海洋細菌の分類
林香倫; 森脇潤; 沢辺智雄; 田島研一; 絵面良男, 日本水産学会大会講演要旨集, 2003, 2003

アワビ消化管から分離されたVibrio halioticoliおよびその類似菌の16S rDNAとgap遺伝子に基づく系統解析
森脇潤; 沢辺智雄; 田島研一; 絵面良男, 日本水産学会北海道支部大会講演要旨集, 2002, 2002

Distribution of Vibrio halioticoli around an Abalone-farming Center in Japan
Reiji Tanaka; Tomoo Sawabe; Mamoru Yoshimizu; Yoshio Ezura, Microbes and Environments, 17, 1, 6, 9, 2002
English

Purification of A Virus-like Particle Causing Growth Suppression of Alexandrium catenella
ONJI Masashi; SAWABE Tomoo; EZURA Yoshio, Bulletin of Fisheries Sciences, Hokkaido University, 52, 3, 135, 138, Dec. 2001
Hokkaido University, English

Retrieval of the species Alteromonas tetraodonis Simidu et al. 1990 as Pseudoalteromonas tetraodonis comb. nov and emendation of description
EP Ivanova; LA Romanenko; MH Matte; GR Matte; AM Lysenko; U Simidu; K Kita-Tsukamoto; T Sawabe; MV Vysotskii; GM Frolova; Mikhailov, V; R Christen; RR Colwell, INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 51, 1071, 1078, May 2001
English

Shewanella japonica sp nov.
EP Ivanova; T Sawabe; NM Gorshkova; Svetashev, VI; VV Mikhailov; DV Nicolau; R Christen, INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 51, 1027, 1033, May 2001
English

Identification of Vibrio halioticoli using 16s rDNA PCR/RFLP (restriction fragment length polymorphism) analysis
Reiji Tanaka; Tomoo Sawabe; Kenichi Tajima; Johan Vandenberghe; Yoshio Ezura, Fisheries Science, 67, 1, 185, 187, Feb. 2001
English

An Evaluation of Viable Staining Dyes Suitable for Marine Phytoplankton
ONJI Masashi; SAWABE Tomoo; EZURA Yoshio, Bulletin of the Faculty of Fisheries, Hokkaido University, 51, 3, 153, 157, Dec. 2000
Hokkaido University, English

New approach in environmentally sound aquaculture industries incorporating the concepts of microbes' utilization
SAWABE Tomoo, 農林水産技術研究ジャーナル, 23, 11, 36, 43, 01 Nov. 2000
農林水産技術情報協会, Japanese

Properties of novel alginolytic marine bacteria and their applications for fisheries science
T Sawabe, NIPPON SUISAN GAKKAISHI, 66, 4, 615, 618, Jul. 2000
Japanese, Book review

Properties of Novel Alginolytic Marine Bacteria and Their Applications for Fisheries Science.
Tomoo Sawabe, 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries, 66, 3, 368, 368, 15 May 2000
Japanese

C-115 異体類の腸内細菌叢と抗ウイルス活性を有する細菌の分離(水圏生態系-4、水処理生態系-3,ポスター発表)
吉水 守; 葛西 砂織; 澤辺 智雄; 絵面 良男, 日本微生物生態学会講演要旨集, 16, 107, 107, 2000
日本微生物生態学会, Japanese

C-214 各種海産無脊椎動物消化管でのVibrio halioticoliの分布(土壌生態系-4、消化管生態系,ポスター発表)
澤辺 智雄; 瀬戸口 央; 田中 礼士; 吉水 守; 絵面 良男, 日本微生物生態学会講演要旨集, 16, 150, 150, 2000
日本微生物生態学会, Japanese

海洋細菌の分類と同定 (総特集 海洋微生物) -- (1章 海洋微生物の分類,同定)
絵面 良男; 田島 研一; 澤辺 智雄, 号外海洋, 23, 12, 17, 2000
海洋出版, Japanese

海産無脊椎動物の消化管内細菌とその機能--アワビとVibrio halioticoliの事例を中心として (総特集 海洋微生物) -- (5章 海洋微生物と相互作用)
澤辺 智雄, 号外海洋, 23, 219, 226, 2000
海洋出版, Japanese

In situ polymerase chain reaction visualization of Vibrio halioticoli using alginate lyase gene AlyVG2
Sugimura, I; T Sawabe; Y Ezura, MARINE BIOTECHNOLOGY, 2, 1, 74, 79, Jan. 2000
English

Cloning and sequence analysis of Vibrio halioticoli genes encoding three types of polyguluronate lyase
Sugimura, I; T Sawabe; Y Ezura, MARINE BIOTECHNOLOGY, 2, 1, 65, 73, Jan. 2000
English

Assignment of Alteromonas elyakovii KMM 162(T) and five strains isolated from spot-wounded fronds of Laminaria japonica to Pseudoalteromonas elyakovii comb. nov and the extended description of the species
T Sawabe; R Tanaka; MM Iqbal; K Tajima; Y Ezura; EP Ivanova; R Christen, INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 50, 265, 271, Jan. 2000
English

Bacillus horti sp. nov., a new Gram-negative alkaliphilic bacillus (vol 48, pg 565, 1998)
Yumoto, I; K Yamazaki; T Sawabe; K Nakano; K Kawasaki; Y Ezura; H Shinano, INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 49, 1951, 1951, Oct. 1999
English, Others

Bacteriological Study on the Coastal Area of the Shimokita Peninsula
OBATA Tomomi; SAWABE Tomoo; TANAKA Reiji; TANIUCHI Yoshikazu; ONJI Masashi; TAJIMA Kenichi; EZURA Yoshio, Bulletin of the Faculty of Fisheries, Hokkaido University, 50, 2, 115, 122, Aug. 1999
Hokkaido University, Japanese

Bacteriological Study on the Mass Mortality of Cultured Hanasakicrab Larvae : Causative bacterial characteristics and infection trial
WATANABE Ken-ichi; YOSHIMIZU Mamoru; OBANA Hiroyuki; KAMATA Ken-ichi; SUGISAWA Teruhumi; SAWABE Tomoo; EZURA Yoshio, Bulletin of the Faculty of Fisheries, Hokkaido University, 50, 1, 19, 32, Mar. 1999
Hokkaido University, Japanese

Classification of alginate lyases by hydrophobic cluster analysis.
Kraiwattanapong Jintana; Ooi Toshihiko; Kinoshita Shinichi; Sawabe Tomoo; Ezura Yoshio, 日本生物工学会大会講演要旨集, 11, 38, 38, 1999
日本生物工学会, Japanese

Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica
Sawabe T.; Makino H.; Tatsumi M.; Nakano K.; Tajima K.; Iqbal MM; Yumoto I.; Ezura Y.; Christen R., International Journal of Systematic Bacteriology, 48, 48, 769, 774, Nov. 1998
An aerobic, polarly flagellated marine bacterium that produces a prodigiosin-like pigment was isolated from the red-spotted culture beds of Laminaria japonica. Five isolates had unique bacteriolytic activity for both Gram-positive and -negative bacteria, which had never been observed among Alteromonas or related species. The isolates were identified as the causative agent of red spot disease of L. japonica seeds. The phenotypic features of the isolates were similar to these of Pseudoalteromonas rubra ATCC 29570T, but they could be differentiated using 10 traits (growth at 37 degrees C, requirement for organic growth factors, bacteriolytic activity, utilization of sucrose, N- acetylglucosamine, fumarate, succinate, D-galactose, L-proline and acetate). The G+C content of DNAs from the isolates was 44-46 mol%. The isolates constitute a new species, distinct from the other Alteromonas and Pseudoalteromonas species, as shown by DNA-DNA hybridization experiments and phylogenetic clustering of 16S rRNA gene sequences, for which the name Pseudoalteromonas bacteriolytica sp. nov. (type strain = IAM 14595T) is proposed. A set of phenotypic features which differentiate this new species from closely related Pseudoalteromonas and Alteromonas species is provided., International Union of Microbiological Societies, English

Vibrio halioticoli sp. nov., a non-motile alginolytic marine bacterium isolated from the gut of the abalone Haliotis discus hannai
SAWABE T., International Journal of Systematic Bacteriology, 48, 573, 580, May 1998
Six alginolytic, facultatively anaerobic, non-motile marine bacteria were isolated from the gut of abalone Haliotis discus hannai. DNA-DNA hybridization data showed that the six strains constituted a single genospecies. Phylogenetic analyses of 16S rDNA sequences indicated that the isolates should be assigned to the genus Vibrio. The phenotypic features of the isolates were closely related to Vibrio fischeri and Vibrio pelagius biovar I, but 13 traits (motility, luminescence, alginase production, lipase production, lysine decarboxylase, indole production, growth in 1 and 6% NaCl and assimilation of five carbon compounds) distinguished these strains from V. fischeri, and 17 traits (motility, growth at 37 degrees C, lipase production, indole production, growth in 1 and 6% NaCl, acid from sucrose and D-sorbitol, and assimilation of nine carbon compounds) distinguished these strains from V. pelagius. The G + C content of the isolates was 41.6-43.1 mol%. According to DNA-DNA hybridization data and 16S rDNA phylogenetic analyses, it was concluded that the six isolates constitute a new species different from any other Vibrio species. The name Vibrio halioticoli sp. nov. (type strain IAM 14596T) is proposed. A set of phenotypic features which enables differentiation of the new species from other species of the Vibrionaceae family is described., International Union of Microbiological Societies, English

Vibrio halioticoli sp. nov., a non-motile alginolytic marine bacterium isolated from the gut of the abalone Haliotis discus hannai
T Sawabe; Sugimura, I; M Ohtsuka; K Nakano; K Tajima; Y Ezura; R Christen, INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 48, 573, 580, Apr. 1998
English

海産食藻動物消化管由来Vibrio属細菌のin situ PCR法による検出--生態解明への応用の可能性 (総特集 バイオマ-カ-による海洋微生物群集の解析)
沢辺 智雄; 杉村 逸郎; 絵面 良男, 海洋, 29, 12, 732, 737, Dec. 1997
海洋出版, Japanese

Novel alginate lyases from marine bacterium Alteromonas sp. strain H-4
T Sawabe; M Ohtsuka; Y Ezura, CARBOHYDRATE RESEARCH, 304, 1, 69, 76, Oct. 1997
English

112 Hydrophobic cluster analysis of 4 newly isolated alginate lyases
Kraiwattanapong J.; Ooi T.; Kinoshita S.; Sugimura I.; Sawabe T.; Ezura Y, 日本生物工学会大会講演要旨集, 9, 8, 8, 1997
日本生物工学会, English

Regeneration from Laminaria japonica Areschoug (Laminariales, Phaeophyceae) protoplasts isolated with bacterial alginase
T Sawabe; Y Ezura, PLANT CELL REPORTS, 15, 12, 892, 895, Sep. 1996
English

細菌由来アルギナーゼを用いて単離したプロトプラストの再生
プラントセルリポート, 15, 892, 1996

ALGINATE DEGRADATION BY BACTERIA ISOLATED FROM THE GUT OF SEA-URCHINS AND ABALONES
T SAWABE; Y ODA; Y SHIOMI; Y EZURA, MICROBIAL ECOLOGY, 30, 2, 193, 202, Sep. 1995
English

赤潮藻類Gymnodinium mikimotoiの増殖に及ぼす田辺湾分離細菌の影響
北海道大学水産研究彙報, 46, 2, 39, 46, 1995

ウニ、アワビ消化管から分離した細菌のアルギン酸分解性
30, 193, 202, 1995

Effect of marine bactera isolated from Tanabe Bay on the growth of marine red tide dinoflagellate,Gymnodinium mikimotoi
46, 2, 39, 46, 1995

Substrate specificity of extracellular alginate lyase produced by marine Alteromonas sp. strain H-4.
Sawabe Tomoo; Oda Yasuhiro; Ezura Yoshio, 日本生物工学会大会講演要旨集, 6, 93, 93, 1994
日本生物工学会, Japanese

APPLICATION OF AN ALGINATE LYASE FROM ALTEROMONAS SP FOR ISOLATION OF PROTOPLASTS FROM A BROWN-ALGAE LAMINARIA-JAPONICA
T SAWABE; Y EZURA; T KIMURA, NIPPON SUISAN GAKKAISHI, 59, 4, 705, 709, Apr. 1993
English

Application of an Alginate Lyase from Alteromonas sp. for Isolation of Protoplasts from a Brown Algae Laminaria japonica.
Tomoo Sawabe; Yoshio Ezura; Takahisa Kimura, Nippon Suisan Gakkaishi (Japanese Edition), 59, 4, 705, 709, 1993
English

PURIFICATION AND CHARACTERIZATION OF AN ALGINATE LYASE FROM MARINE ALTEROMONAS-SP
T SAWABE; Y EZURA; T KIMURA, NIPPON SUISAN GAKKAISHI, 58, 3, 521, 527, Mar. 1992
English

海洋性┣DBAlteromonas(/)-┫DB sp.の産生するアルギン酸リアーゼの精製と性質
澤辺 智雄; 絵面 良男; 木村 喬久, 日本水産学会誌, 58, 3, 521, 527, 1992

リシリコンブ穴あき病葉体から分離されたアルギン酸分解菌の性状
日本水産学会誌, 58,141-145, 1992

CHARACTERIZATION OF AN ALGINOLYTIC MARINE BACTERIUM FROM DECAYING RISHIRI-KOMBU LAMINARIA-JAPONICA VAR OCHOTENSIS
T SAWABE; Y EZURA; T KIMURA, NIPPON SUISAN GAKKAISHI, 58, 1, 141, 145, Jan. 1992
English

水圏微生物学の基礎
濵﨑 恒二; 木暮 一啓; 澤 辺智雄; 澤辺 桃子; 鈴木 聡; 砂村 倫成; 永田 俊; 春田 伸; 福田 秀樹; 美野 さやか; 和田 実; 濵﨑 恒二; 木暮 一啓
恒星社厚生閣, 25 Sep. 2015, 4769915683, 280, [Joint work]

The Prokaryotes 4th edition
B. Gomez-Gil; C.C. Thompson; Y. Matsumura; T. Sawabe; T. Iida; R. Christen; F.T. Thompson; T. Sawabe, Family Vibrionaceae
Springer, 2014, [Contributor]

海藻バイオ燃料
澤辺 智雄, マリンビブリオを活用した海藻からのエタノール生産
CMC出版, 2011, [Contributor]

The Biology of Vibrios
SAWABE Tomoo, The mutual partnership between Vibrio halioticoli and abalones
ASM Press, 2006, [Contributor]

The biology of vibrios
Thompson, Fabiano Lopes; Austin, B. (Brian); Swings, J. G.; American Society for Microbiology
ASM Press, 2006, 1555813658, xiii, 423 p., English

キャリアマネジメントセミナー, 2024年, 修士課程, 水産科学院

ステップアップキャリア形成Ⅰ, 2024年, 修士課程, 水産科学院

ステップアップキャリア形成Ⅱ, 2024年, 修士課程, 水産科学院

海洋生物工学特論Ⅱ, 2024年, 修士課程, 水産科学院

微生物学実験, 2024年, 学士課程, 水産学部

海洋微生物学, 2024年, 学士課程, 水産学部

水産科学英語Ⅰ, 2024年, 学士課程, 水産学部

水産科学英語Ⅱ, 2024年, 学士課程, 水産学部

養殖コンブの疾病調査
2000

Research on disease of Laminaria
2000

Studies on hydrogen producing machineries of marine vibrios
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2019 - 31 Mar. 2023
澤辺 智雄; 美野 さやか
化石燃料代替エネルギー生産技術開発は，学術的・社会的要請が高い地球規模課題である。海洋バイオマスのエネルギー変換技術の開発もその一つであるが，アルギン酸などの難燃料化成分が多く，その完全変換にはさらなる技術革新が必要である。我々が見いだした新規マリンビブリオは，Hyf複合体を核とするギ酸水素リアーゼ（FHL）複合体を介した独特の水素代謝を示し，海藻由来の種々の糖を水素化する。しかし，マリンビブリオ触媒の水素生成能を高める分子育種基盤の構築は発展途上であり，海洋バイオ水素生産性向上のボトルネックでもある。Hyf複合体の分子レベルで特徴を理解するためには，マリンビブリオは恰好の生物材料であるため，本研究では，マリンビブリオが有する活性型Hyf複合体の特徴を理解し，多彩な海洋バイオマスからのより効率的な水素生成が可能な海洋微生物触媒の構築にフィードバックさせる知見を得ることを目的に研究を進めた。その結果以下の成果を得た。
①バイオ水素生成マリンビブリオ全種の完全ゲノムの取得
水素生成能が観察されているほぼ全種を含む17種のビブリオ科細菌の完全ゲノムを取得した。遺伝子構造に基づき，主に3タイプに分かれることを明らかにした。
②水素生成マリンビブリオ触媒の能力比較
上記17種のうち，水素生成が確認できる12種について，グルコースを基質とした場合の水素生成プロファイルを調べたところ，ギ酸の再取り込み量が水素生成と相関することを明らかにした。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 19H03041

Studies on echinoderm's microbiome
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
28 Jun. 2019 - 31 Mar. 2021
Sawabe Tomoo
Using sea cucumber as a model of marine invertebrate, 1) pioneer and/or first colonizer candidates were mined, 2) these bacterial strains and genome data were collected, and 3) these MALDI-TOF-MASS data were accumulated.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hokkaido University, 19K22262

Creation of Molecular Biotechnology Basis for Marine Vibrios Toward Innovation of Marine Biohydrogen Production
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2016 - 31 Mar. 2020
Sawabe Tomoo
Aims of the study are to understand physiology and genomics of hydrogen production marine vibrios. We did achieve 1) optimized synthetic medium, 2) physiological and genome comparison among hydrogen producing marine vibrios, 3) a RNA-Seq, and 4) effects of nitrogen fixation on hydrogen production. We also discovered a new marine vibrio which is capable of producing hydrogen from alginate.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 16H04976

Developments of marine vibrio biocatalysts in efficient conversion of seaweed biomass based on consolidated biological processing approach
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
01 Apr. 2016 - 31 Mar. 2018
Sawabe Tomoo
An aim of the study is to develop marine microbial biocatalysts implemented multiple substrate utilizing phenotypes based on consolidated biological processing (CBP) in efficient biofuel production. We created new marine vibrio biocatalysts showing 1) polyuronide-sugar alcohol-glucan utilizing phenotype and 2) higher ethanol producing phenotype from D-galactose.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 16K14978

Creation of A Light-Driven Marine Vibrio Factory for Biofuel Production
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
01 Apr. 2014 - 31 Mar. 2016
Sawabe Tomoo; HOSOKAWA MASASHI
An aim of the study is to develop light-driven marine vibrio biocatalysts in efficient biofuel production. We performed 1) gene expression of a Nonlabens sediminis proteorhdopsin gene, and 2) a cloning of a myxol synthetic gene cluster of Nonlabens ulvanivorans. We also accumulated fundamental knowledge on CRISPR/Cas system for further developments of a genome editing approach working in marine vibrio cells.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 26660168

Development of high perfomanced hydogen production system using marine vibrios as biocatalysts
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2013 - 31 Mar. 2016
Sawabe Tomoo; HOSOKAWA MASASHI; NAKAGAWA SATOSHI
Aims of the study are to establishment of 1) continuous culture system and 2) biorefinery of hydrogen producing marine vibrios as biocatalysts. We did succeeded 1) continuous hydrogen production for 70 days with appropriate hydrogen producing rates and yields, 2) alginate-hydrogen conversion using 2 kinds of biocatalysts, and 3) bioprospects of new and rare carotenoid producers and their genome information.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 25292122

Creation of marine vibrio cells with minimun gene sets for efficient biofuel production
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
2011 - 2013
SAWABE Tomoo; HOSOKAWA Masashi; NAKAGAWA Satoshi
In maintaining a sustainable ecosystem in this period of global warming, development of key technologies in the field of renewable energy sources has become an important challenge; a method of biofuel production from marine biomass could be one of the most crucial technologies in the future. In this study, we performed global transcriptome, fermentation product profiling, and metabolic engineering to design marine vibrio cells with efficient ethanol production from marine biomass.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, Principal investigator, Competitive research funding, 23658172

Nanometer-sized sulfides of a deep-sea vent animal: comprehensive analysis of metal formation mechanisms.
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
2011 - 2012
NAKAGAWA Satoshi; TAKAI Ken; SAWAB Tomoo; SUZUKI Yohey
The main objective of this study is to reveal mechanisms by which deep-sea vent animals and their symbionts sense and respond to environmental fluctuations. By using the high-throughput sequencing technique, meta-transcriptome analysis from a deep-seahydrothermal vent gastropod species was performed. This study lead to the identification of biological processes underlying physiological adaptation to deep-sea vent environments.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 23658154

Establishment of A Marine Vibrio Platform in The Bioconversion ofSeaweed Carbohydrates to BioEthanol
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2009 - 2012
SAWABE Tomoo; OJIMA Takao; NAKAGAWA Satoshi
In maintaining a sustainable ecosystem in this period of global warming, development of key technologies in the field of renewable energy sources has become an important challenge; a method of biofuel production from marine biomass could be one of the mostcrucial technologies in the future. In this study, we investigated genome wide metabolic pathway prediction, functional analysis of the genes responsible for the alginate degradation and ethanol production, and the transcriptomic analysis in two unique marine vibrios, both of which are capable of producing bioethanol from seaweed carbohydrates. We successfully reconstructed the metabolic pathway with functional and transcriptomic information as a marine vibrio platform.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 21380129

Towards a molecular understanding of symbiotic mechanism of chemoautotrophs in deep-sea hydrothermal vent environments.
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)
2008 - 2010
NAKAGAWA Satoshi; TAKAI Ken; MAKITA Hiroko; SAWABE Tomoo
The main objective of this study is to reveal the molecular mechanisms underlying symbiont-host relationship in deep-sea hydrothermal vent environments. We have focused on sugar chains, glycans. We have succeeded in detection, profiling, structure determination of glycans for both symbionts and their host animals. Glycans having quite unique structures were identified.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (A), Japan Agency for Marine-Earth Science and Technology, Coinvestigator not use grants, Competitive research funding, 20687003

Study on the metabolic pathways for seaweed polysaccharides in abalone
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2007 - 2010
OJIMA Takao; INOUE Akira; TANAKA Hiroyuki; SAWABE Tomoo
Herbivorous marine gastropods like abalone and sea hare possess various polysaccharide-degrading enzymes which can degrade seaweeds' polysaccharides such as alginate, laminaran, mannan, xylane, and cellulose to oligo- and monosaccharides. These saccharides are considered to be metabolized via glycolytic pathway and TCA cycle. In the present study, we isolated several polysaccharide-degrading enzymes, e.g., alginate lyase, laminarinase and mannanase, from abalone and sea hare and investigated their biological roles in the metabolism of seaweeds' polysaccharides in gastropods.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 19380117

Produce a safe and worry free oyster for whole season
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2005 - 2008
YOSHIMIZU Mamoru; TAJIMA Kenichi; NISHIZAWA Toyohiko; SAWABE Tomoo; KASAI Hisae
20℃以上に調温した0.3mg/Lの塩素を含む電解海水あるいは中圧紫外線処理海水を用い、V. alginolyticus優勢キートセロスを給餌しながら浄化すれば、カキが痩せることなく、大腸菌、ノロウイルスおよびV. parahaemolyticusを排除できると考えられる。さらに安全性を確保するために2,000気圧の高静水圧をかけて脱殻を行えば、安全性はより向上する。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 17380110

Dynamics of primary productivity in a subarctic coastal area affected by a large river discharge.
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2003 - 2005
KUDO Isao; MONTANI Shigeru; SAITOH Sei-ichi; SAWABE Tomoo
Chemical substances and biochemical parameters were measured in the subarctic coastal region which receives fresh water and nutrients from the Tokachi River, one of the largest river in Japan. River discharge increased ten times after April due to a spring thaw and continued until June. In the coastal region, nutrients were depleted after a spring bloom which occurred in April. River plume area showed an elevated concentration of nutrients and iron originated from the Tokachi River. High concentration of chlorophyll a was observed only in the river plume because outside the plume nutrients were depleted. The mainstream of the Tokachi River and its tributaries showed different concentration of nutrients. This may be attributed to the different type of land use, i.e., farming, dairy farming, forest and city sewage plants. We also investigated the heterotrophic microbial process (microbial loop) which is related to the fate of organic carbon produced by phytoplankton. The abundance of heterotrophic bacteria was higher in the river plume than off shore region. Dissolved organic carbon was also higher in the plume. Riverine organic matter may accelerate the growth of heterotrophic bacteria in the plume. The mortality of bacteria was investigated by a dilution culture experiment. Virus infection was accounted for more than 80% of the mortality. The regenerated organic carbon may contribute to the further bacterial production.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, Coinvestigator not use grants, Competitive research funding, 15310019

Study on the Elimination Method of Small Rounded Structure Virus (SRSV) from Oyster.
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2002 - 2004
YOSHIMIZU Mamoru; TAJIMA Kenichi; NISHIZAWA Toyohiko; SAWABE Tomoo
Oysters (Crassestrea gigas) harvested from major cultivation areas are commonly contaminated with Escherichia coli. Also, some places, oyster concentrates small rounded structured virus (SRSV ; norovirus). It's very important to remove the E.coli, food born bacteria and norovirus. E.coli has to be removed from oysters during oyster purification by re-laying in a non-polluted seawater. Although sodium hypochlorite or UV irradiation is used to disinfect seawater, it's difficult to treat large volumes of seawater. Recently the bactericidal and virucidal effects of hypochlorite produced by electrolysis of seawater were examined against pathogenic bacteria and viruses.
SRSV (norovirus) is not able to replicate in the established cell line, and its difficult to determine the virus infectivity. This virus is belonging to family Caliciviridae, and cat pathogenic virus ; FCV that is able to replicate in cat cell line. In this study, virucidal effects of UV irradiation, hypochlorite and high pressure on FCV infectivity were studied to eliminate the FCV from oyster. Stability of FCV in sea water and intestinal contents of oyster was also studied.
FCV was inactivated at the dose of 1.0x10^4 micro W・sec/cm^2, electrolyzed seawater chlorine concentration controlled at 0.20〜0.4mg/L, and 800MPa・40℃・5min FCV infectivity decreased rapidly in sea water at the temperature 20℃ or high. In the intestinal contents of oyster, FCV was inactivated by antiviral substances produced by intestinal bacteria. These results indicate that combination of 3 conditions ; (1)using a electrolyzed sea water containing the 0.3 mg/L hypochlorite, (2)harvest in sea water at the temperature 20℃ or high, (3)remove the shell using a 800 MPa・at 40℃ for 5 min. Seawater electrolyzers have ability to treat large volumes of water and cost performance of this method is better than that of UV irradiation. From these results, it is considered that electrolysis of seawater could be a useful method for post harvest elimination of norovirus from oysters.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 14360102

Development the Seawater Sterilizer Adapted for HACCP System for Fisheries Field from Fishing to Processing
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2001 - 2004
YOSHIMIZU Mamoru; SAWABE Tomoo; UENISHI Toshio
In Japan, because many seafood products are distributed without being heated, generally there is a danger of their quality deteriorating sharply with the passage of time at each stage after fishing. This makes temperature control important in order to inhibit growth of pathogens and spoilage organisms. Reliable hygiene and sanitation controls at all stages from fishing until products reach consumers are vital. The basic approach involves, at every stage from fishing, prevention of products pathogens, maintaining temperatures below set levels, thoroughly controlling the water and ice used, preventing damage to raw material, and minimal processing time. Clean crushed ice is stored in a sanitary ship hold. Fish are preserved in the hold filled with clean seawater and ice.
Establishment of seawater disinfection method is very important, because Eschericia coli and coli forms were isolated from seawater in almost of all fishing port. A pathogen-free water source is essential, and treatment with ultraviolet light is used widely. UV treatment is limited the volume. Hypochlorite produced by electrolysis of seawater showed bactericidal and virucidal effects. This method can easily treat large volumes of seawater, and is suitable for disinfecting sea water for hygiene and sanitation at a fishing port. Dssinfection of seawater pomp up from the fishing port was investigated using electrolyzed seawater. Viable bacterial counts of electrolyzed seawater from port were decreased and this water was useful to disinfect the deck and hatch of fishing boat, fish selection board, quay, and the market floor at a fishing port.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, Coinvestigator not use grants, Competitive research funding, 13556027

Analysis, distribution and function of unnatural glycerophospholipids in marine organisms
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2001 - 2003
ITABASHI Yutaka; ANDO Yasuhiro; SAWABE Tomoo
The aim of this study was to clarify the distribution and physiological function of unusual glycerophospho- and glycolipids in marine organisms. For this purpose, chiral phase HPLC, reversed-phase HPLC and reversed-phase HPLC in conjunction with electrospray ionization mass spectrometry(ESI-MS) were applied to the separation and identification of stereoisomeric and regio-isomeric glycerolipids. Using these techniques, we found an unnatural phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-3'-sn-glycerol, R, R configurations at the two chiral centers) from various kinds of microorganisms including marine bacteria. The R, R isomer content increased with increasing culture temperature. The results strongly suggest that the bacteria adapt to temperature by alternating not only the degree of unsaturation but also the stereoisomer composition of phosphatidylglycerols. In the present study, we also investigated the separation and identification of the reverse isomers of glycerophospholipids (PtdCho,PtdEtn,PtdIno) and glycoglycerolipids (MGDG,DGDG,SQDG) by reversed-phase HPLC/ESI-MS. These glycerolipids were chromatographed as intact forms or 3,5-dinitrophenylurethanes of the 1,2-diacyl-sn-glycerols derived from the parent molecules. Excellent resolution was achieved for reverse isomers of very different pairs of acyl groups, such as 20:5-16:0 and 22:6-16:0, on highly efficient C18 columns. Using the method, we analyzed the glycerolipids from some fish and marine algae, and found that only MGDG from marine red algae, such as Porphyra yezoensis and Gracilaria verrucosa, gave two clearly resolved peaks, representing the reverse isomers of 20:5(sn-1)/16:0(sn-2) and 16:0(sn-1)/20:5(sn-2), and 20:4(sn-1)/16:0(sn-2) and 16:0(sn-1)/20:5(sn-2), respectively. The present method provides the first direct demonstration of the occurrence of reverse isomers in natural glycoglycerolipids and should be helpful in promoting work on the biosynthetic pathway and physiological importance of the reverse isomers in the red algae.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 13460088

アワビ消化管内細菌叢の人工制御に関する研究
科学研究費助成事業 若手研究(B)
2001 - 2002
澤辺 智雄
本年度の成果は以下の通り。
1.アワビ消化管内細菌叢の抗生物質添加飼料を用いた人工的置換
Vibrio halioticoliは、ペニシリン系、セファロスポリン系、およびクロラムフェニコール(CP)に高い感受性を示した。この中で最小発育阻止濃度の低いベンジルペニシリン(PCG)とCPをそれぞれ0.5-50mg/50gおよび1.5-150mg/50g飼料となるように混合したアワビ配合飼料を1ヶ月間エゾアワビに給餌し、アワビ消化管からのV. halioticoliの除去を試みた。その結果、50mg PCGおよび150mg CP添加飼料給餌区でV. halioticoliの比率は30%から0.04%に減少し、総菌数は10^8cells/9(消化管)から2桁減少した。しかし、生菌数は減少せず10^6CFU/g(消化管)を維持していたが、飼育試験終了時にはPolaribacter sp.が優占し、細菌叢は変化した。次に、アワビ消化管内細菌叢の完全除去を目指し、PCG-CP添加飼料給餌区で残存したPolaribacter sp.に高い感受性を示すセフォタキシム(CTX)を添加したPCG-CP-CTX添加飼料を調製し、飼育試験を試みたが、総菌数および生菌数を著しく減少させる条件を見いだすまでには至らなかった。
2.緑色蛍光タンパク質(GFP)標識V. halioticoliにおけるGFP発現条件の検討
GFP標識V. halioticoli株について細胞単位でのGFP蛍光発現条件の至適化やGFP発現細胞の検出限界時間などを検討した。その結果、GFP発現細胞の比率は培養温度15℃で高く、また、嫌気培養条件(ガスパック法)下においては、好気条件下よりも比率は10%低下するものの、GFP発現細胞は観察された。さらに、15℃-好気条件下でGFPを発現させた細胞は、15-25℃下では滅菌海水中で4日以内までは正確に検出できた。このGFP標識株を用いたトレーサー実験が今後の課題となった。
日本学術振興会, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 13760134

Studies on survival strategy of Flexibacter sp., the causative bacterium of spotting disease of sea urchin Strongylocentrotus intermedius
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
2000 - 2002
TAJIMA Kenichi; SAWABE Tomoo
The survival in low water temperature, detection method in VBNC state and resuscitation from VBNC condition of the bacterium Flexibacter sp., the pathogen of spotting disease of sea urchin Strongylocentrotus intermedius, were examined. The important results obtained were as follwos:
1. The pathogen lost its colony forming ability in 75% ASW at 5℃ after 15 days of incubation. This state was clarified as VBNC state. In VBNC state the bacterial cells became short rod or round in shape and lost its pathogenicity to sea urchin.
2. A primer set was constructed based on the 16S rRNA sequences for detection of the bacterium. The primer set was specific for the bacterium.
3. The VBNC cells resuscitated by temperature up-shift from 5° to 25℃ in 75% ASW in the presence of 0.0017% (approximately 60μM Fe) ferric chloride solution and in 75% ASW containing homogenated sea urchin. The morphology of the resuscitated cell was similar to that of the original cell, they showed positive reaction in agglutination test against rabbit antiserum moreover, the cells regained pathogenicity to sea urchin.
From the above results it is clear that during low water temperature the cells enter into VBNC state, but survive in that condition by adhering to surface fo sea urchin. The findings also clearly indicate that when the water temperature increases the VBNC cells resuscitate and cause disease to the sea urchin.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), HOKKAIDO UNIVERSITY, Coinvestigator not use grants, Competitive research funding, 12660159

Functional improvement of fish myofibrillar protein by conjugation with alginate oligosaccharide.
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
2000 - 2001
SAEKI Hiroki; SAWABE Tomoo; NAKAMURA Soichiro
Fish meat has excellent food functionalities such as gel-forming ability, emulsifying ability, and water-holding ability. However, fish myofibrillar ptotein (Mf) is thermally and chemically less stable than that of other vertebrates and its functional properties are rapidly impaired with progress of protein denaturation. To improve the functional properties and the thermal stability, Mf was conjugated with alginate oligosaccharide (AO : Mean degree of polymerization=6) through the Maillard reaction under controlled humidity states.
1. The improved functional changes of Mf by the conjugation with AO are as follows : (1) The solubility of Mf in low salt concentration (<0.16M) was significantly improved by conjugation with AO and the ionic strength dependence of the solubility was completely lost. (2) The water soluble Mf thus obtained had excellent thermal stability (no aggregation at 80 ℃ for 3h). (3) The emulsifying properties of Mf was also improved by conjugation with AO. (4) Good digestivity of Mf was remained after the conjugation.
2. Bactertial mutagenesis tests and animal dose test were examined to evaluate the food safety of Mf-AO conjugate. (1) No mutagenecity of Mf-AO conjugate was confirmed by rec-assey and Ames-test. (2) Mf-AO conjugate was nontoxic for oral administration to rats.
The results of this study indicate that the conjugation with AO with alginate oligosaccharide through the Maillard reaction is a good manner to improve food functionalities of fish meat as a foodstuff.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), HOKKAIDO UNIVERSITY, Coinvestigator not use grants, Competitive research funding, 12660182

海洋細菌の遺伝子産物大量発現系の開発
科学研究費助成事業 奨励研究(A)
1999 - 2000
澤辺 智雄
本年度は、海洋細菌由来のプラスミッドの探索とそれらのプラスミッドを用いた形質転換の可能性について検討し、以下の結果を得た。
1.海洋細菌由来新規プラスミッドベクターの探索
海水試料から分離した100株の海洋細菌からプラスミッド様核酸を保持するの菌株の探索を行い、5菌株がプラスミッド様核酸を有していた。前年度の成果により、3菌株のプラスミッド保持菌株を見いだしたが、本研究課題で計8菌株が得られた。これら8菌株のプラスミド様核酸に抗生物質耐性マーカー遺伝子が存在するか否かを調べるために、エレクトロポレーション法により、大腸菌JM109株の形質転換を試みた。その結果、OM918-2-6株およびH8株のプラスミッドが、大腸菌内で複製可能で、しかもアンピシリン耐性遺伝子がコードされている可能性が示唆された。
2.Pseudoalteromonas elyakoviiのアルギン酸分解酵素遺伝子を高発現する海洋細菌宿主の探索
P.elyakoviiからクローン化したアルギン酸分解酵素遺伝子alyPEECを挿入した3種類のプラスミッド(pTPB24、pTPB31、pCD11)を用い、2種のアルギン酸非分解性海洋細菌P.haloplanktis IAM12915^TおよびP.tetraodonis IAM14610^Tをエレクトロポレーション法により、形質転換することを試みたが、いずれのプラスミッドとも両海洋細菌で複製しえなかった。
本研究で見いだされたアンピシリン耐性プラスミッドの遺伝子地図の作成とこれらのプラスミッドを安定して保持、複製できる海洋細菌宿主の探索が急務となった。
日本学術振興会, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 11760129

"Study on symbiotic relationship between gut microbes and marine herbivores"
Grants-in-Aid for Scientific Research(基盤研究(B))
1997 - 2000
Yoshio EZURA; Tomoo SAWABE; Mamoru YOSHIMIZU; Kenichi TAJIMA
Aim of the project has been orientated to clarify physiological and ecological functions of gut microbes living in gut of marine herbivores. The project has directed to obtain many excellent results for understanding an abalone-gut microbes interaction as follows ;1. Changes of the gut microflora of abalone were corresponding to a time when a feeding behavior of abalone was changed.2. One of the dominant microbes in the gut of abalone was Vibrio halioticoli, which was identified as a new species of Vibrio.3. Three kinds of V.halioticoli specific identification methods were developed based on 1) 16S rDNA PCR/RFLP, 2) colony hybridization, and 3) in situ PCR.4. Use of the V.halioticoli-specific identification method clarified the interesting distribution.1) V.halioticoli was distributed in seawater in/around an abalone farm, and likely to attach on a surface of microalgae. The bacterium may be reached with the microalgae into the gut of abalone. A change of gut environments creating by a change of the feeding behavior caused the bacterium dominancy in the gut.2) V.halioticoli was also distributed in abalones, H.discus discus, H.diversicolor supertexta, and H.diversicolor diversicolor ; and a turban shell, Turbo cornatus, living in Japan. The bacterium was not isolated from Australian, South African and French abalones.5. Fingerprinting analysis suggested an occurrence of host specific polymorphism among V.halioticoli strains isolated from various abalones.6. Four genes encoding alginate degrading enzymes of V.halioticoli IAM14596^T were cloned and sequenced.7. Preliminary experiment for an artificial substitution of gut microflora of abalone Haliotis discuss hannai using antibiotics-containing diet resulted that an occupation rate of V.halioticoli in the gut of the abalone was decreased below 0.1%.8. Main fermentation products from alginate by V.halioticoli IAM 1459^T were determined as acetic acid and formic acid.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 09460081

Development of Water Disinfection Systems for Seed Production Facilities
Grants-in-Aid for Scientific Research(基盤研究(B))
1997 - 2000
Mamoru YOSHIMIZU; 渡辺 研一; 三村 元; 上西 敏夫; Tomoo SAWABE; Yoshio EZURA; Toshio UENISHI; Kenichi WATANABE; Hajime MIMURA
Water supplies often provide an efficient means for the introduction and spread of infectious desease. Pathogen-free water sources are often essential for success in aquaculture. The surface waters commonly used in aquaculture come from coastal waters or rivers and may contain the fish pathogens. Such open water supplies should be used prior treatment to kill the fish pathogens. Disinfection of wastewater before discharging is better for the environment.Usually, the treatment systems make use of high efficiency sand filters to clarify the water before treatment with ultraviolet(UV)light or ozonation. Fish pathogens are divided into 2 groups based on their sensitivity to UV and TROs. Sensitive viruses include infectious hematopoietic necrosis virus(IHNV), Oncorhynchus masou virus(OMV), lymphocystis virus(LCDV)and Japanese flounder rhabdovirus(HIRRV), and Gram-negative bacteria. These microorganisms are inactivated or killed by treatment with 10^4μW・sec/cm^2(UV dose)or 0.1mg/L(TROs)for 1min. Resistant viruses include infectious pancreatic necrosis virus(IPNV), marine birnavirus, reovirus and fish nodavirus, Gram-positive bacteria, fungi and parasite which are inactivated with a dose of 106μ W・sec/m^2 or 0.5mg/L(TROs)for 1min. Ozonated seawater containing TROs is toxic to fixh, thus it should be removed by charcoal before using.Bactericidal and virucidal effects of hypochlorite produced by Electrolysis of seawater(salt water)are examined. More than 99.99% cells of V.anguillarum and A.salmonicida were killed when the bacteria were exposed to 0.1 mg/L hypochlorite for 1 min. Yellowtail ascites virus(YAV)and hirame rhabdovirus(HIRRV)were inactivated>99099% after treatment with 0.58 mg/L hypochlorite for 1 min. Using for wastewater, more than 99% of viable bacteria in waste-seawater was reduced treated with 0.5mg/L of total residual chlorite for 1min. The bactericidal effects of electrolysis were almost same as that of ultraviolet irradiation(1.0×10^5μW・sec/cm^2)or ozonization(TROs 0.5mg/L, 1min)of seawater. This method is easy to treat large volume of water, so it suits to disinfection of wastewater.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 09556041

海産食藻動物の腸内細菌のアルギン酸分解特異性と栄養生理学的役割に関する研究
科学研究費補助金(奨励研究(A))
1996 - 1996
澤辺 智雄
本年度に得られた成果は以下の通りである。I.ウニ・アワビ消化管由来アルギン酸分解菌の種構成と分解特異性エゾバフンウニ消化管から分離したアルギン酸分解菌はその一般性状からVibrio属と同定されたものが大半を占めていたが、代表株についてDNA-DNA相同性を測定した結果、少なくとも2菌種以上が混在していることが明らかとなった。一方、エゾアワビ消化管から分離したアルギン酸分解菌は非運動性のVibrio属類似細菌が主体をなしていた。これらの菌群は16SrDNA塩基配列による分子系統解析およびアルギン酸分解性Vibrio属標準株とのDNA-DNA相同性を測定した結果、Vivrio属の新種であることが明らかとなった。また、ウニ消化管由来アルギン酸分解菌のアルギン酸分解特異性はアルギン酸を構成するpolyMおよびpolyGいずれのホモポリマーとも分解する菌株が30%以上を占めているのに対し、アワビ分離株ではpolyGブロックに対する分解性の強い菌株が30-70%占めていた。ウニ自体はアルギン酸分解酵素を分泌しないことから、消化管内細菌がアルギン酸分解の大部分を担っていると考えられた。一方、アワビはpolyM特異的な分解酵素を分泌することから、アワビ消化酵素で分解されにくい部分を分解する消化管内細菌が定住していることが示唆された。II.アルギン酸分解酵素の特性ウニ由来アルギン酸分解菌代表株Ud10株は基質特異性を示さないNaCl要求性の強い酵素を誘導的に産生していた。一方、アワビ由来アルギン酸分解菌A431株はpolyG特異的分解酵素以外にも、基質特異性の異なる6種類以上の酵素を産生しており、その中でpolyMに強い特異性を示す分解活性画分およびpolyGに強い特異性を示す分解活性画分の部分精製物について酵素化学的性状を調べた。それぞれの画分の至適反応温度は40℃および30℃と異なっていたが、至適反応温度(pH7.5)および塩類の要求性(100mM NaCl)は同様の性質を示した。
文部科学省, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 08760172

海洋における溶存鉄濃度を支配する因子としての天然有機リガンドの存在とその起源
科学研究費補助金(基盤研究(C))
1996 - 1996
久万 健志; 志賀 直信; 澤辺 智雄
1、(1)紫外線を照射して有機物を除去した海水の含水酸化鉄の溶解度(20℃)は、天然海水に比べ著しく低く、その値は【less than or equal】0.1nMであった。(2)含水酸化鉄の溶解度の高い沿岸水(天然有機リガンドを含む)と紫外線を照射して得られた溶解度の低い沿岸水(有機リガンドを除去した)における沿岸性植物プランクトン(珪藻)の鉄添加増殖実験から、紫外線を照射した海水ではその増殖速度は遅くなる。このことは、天然海水に存在する有機リガンドが溶存鉄濃度を高め、より生物に使われやすい溶存形態になっていると思われる。2、(1)北海道噴火湾でのスプリングブルーム前の鉛直混合期(2月上旬)には、海水における含水酸化鉄の溶解度は低く、ほぼ一定値(0.2-0.3nM)を示した。しかしながら、ブルーム最盛期(3月中旬)には20m以浅で最大値(0.7-0.8nM)に達し、ブルーム終了後においても高い溶解度が維持された。これは、植物プランクトン、バクテリア等から放出された有機リガンドによるものと推察され、藻類による鉄の取り込みに重要な影響を与えていると考えられる。しかしながら、溶解度とクロロフィル-a濃度とは必ずしも相関性がなく、すべての植物プランクトンが有機リガンドを放出しているとは考えられない。(2)ブルーム前後における珪藻類優占種の変動は、ブルーム前から最盛期(2月〜3月中旬)にかけてThalassiosira nordenskioldiiが最も優占し、最盛期以降(3月中旬以降)にChaetoceros compressumやChaetoceros concavicorneに遷移した。このことは、ある種の植物プランクトンが有機リガンドを放出している可能性を示している。またある種の海産性バクテリアにもシドロフォアのような有機リガンドを放出することが確認された。以上の結果から、沿岸海水には3価鉄と溶存有機鉄錯体を形成する有機リガンドが存在しており、沿岸域での生物生産に重要な役割を果たしていると
文部科学省, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 08640617

Establishment of the method for detection of virus specific gene and application of this methods for control of fish viral disease.
Grants-in-Aid for Scientific Research(一般研究(B), 基盤研究(B))
1994 - 1996
Mamor YOSHIMIZU; Toyohiko NISHIZAWA; Tomoo SAWABE; Kenichi TAJIMA; Yoshio EZURA
Viral disease is a serious problems of fish in hatchery. Especially, viral nervous necrosis of marine fish and infectious hematopoietic necrosis of salmonids are difficult to control. In this study, we studied and established the highly sensitive and specific diagnostic method to detect the virus specific gene using polymerase chain reaction (PCR) method. Results obtained in this study are summarized as follow.1. Striped jack nervous necrosis virus (SJNNV) coat protein gene and infectious hematopoietic necrosis virus (IHNV) N protein gene were amplified by polymerase chain reaction (PCR) an could apply for the specific and sensitive diagnosis of VNN and IHN.2. Rainbow trout embryo (RTE-2) and sea bass kidney (SBK-2) cell lines were selected for the isolation of IHNV and NNV.3. Method of the simultaneous detection and identification of both IHNV and infectious pancreatic necrosis virus (IPNV) in a single PCR reaction was developed.4. Fish nodaviruses were clasified into 4 clusters by molecular phylo-genetic analysis of the coat protein gene.5. Combination of RT-PCR and antibody screening by ELISA was effective method for the identification of nervous necrosis viurs carriers in a flounder brood stock.
Ministry of Education, Culture, Sports, Science and Technology, 一般研究(B), 基盤研究(B), 北海道大学, Competitive research funding, 06454095

海産藻類に感染するウイルスの探索とその生態に関する研究
科学研究費補助金(奨励研究(A))
1995 - 1995
澤辺 智雄
本年度に得られた成果は以下の通りである。1.海産微細藻類感染ウイルスの探索と特性北海道噴火湾沿岸海水から海産微細藻類の増殖に影響を与えるウイルスの探索を試み、濃縮した海水の0.45μmろ過性画分に渦鞭毛藻、Alexandrium catenella、Gymnodinium mikimotoiおよびProrocentrum sp.の3藻類の増殖を抑制する現象を見出した。A.catenellaおよびG.mikimotoiに対し増殖抑制効果を示すろ過性因子は、今年度(1995年)と昨年度の9月〜10月に採水した海水に認められ、その抑制効果は40〜90%の範囲であった。また、Prorocentrum sp.に対し増殖抑制効果を示すろ過性因子は1993年9月〜10月に採水した海水に見い出され、その抑制効果は20〜30%であったが、今年度は観察されなかった。いずれの藻類増殖抑制因子とも孔径0.45μmおよび孔径0.22μmのフィルターを通過させた後でも抑制効果は持続した。同様の増殖抑制因子は調査を行なった3年間とも9月〜10月にのみ見出され、季節変動が観察された。Prorocentrum sp.、A.catenellaおよびG.mikimotoiの各増殖抑制因子は0.22μm以上の孔径のフィルターを増殖抑制効果の低減なしに通過したが、孔径0.05μmフィルターでのろ過では増殖抑制効果が消失した。さらに、1993年の海水試料から検出されたProrocentrum sp.、A.catenellaの増殖抑制因子は50℃、30分の加熱およびRNase処理により増殖抑制効果が消失した。また、超遠心沈降画分のネガティブ染色像の観察により長さ約400nm、幅約30nmの屈曲棒状のウイルス様構造体が観察された。これらの特性から、本藻類増殖抑制因子が植物ウイルスに多く見られる屈曲棒状のRNAウイルスである可能性が示唆された。II.海産大型藻類感染ウイルス培養系の構築マコンブプロトプラストの連続培養系を考案し、プロトプラストの再生率を向上させることができた。この培養系はマコンブ感染ウイルスの分離に応用が期待される。
文部科学省, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 07856018

海洋細菌由来のアルギン酸分解酵素を利用した褐藻細胞のプロトプラスト化に関する研究
科学研究費補助金(奨励研究(A))
1993 - 1993
澤辺 智雄
海洋細菌Alteromonas sp.H-4株の培養上清から精製したアルギン酸分解酵素を5mM HEPES-25mM MgCl_2-50%天然海水-0.7Mマンニトールに対し透析し、アルギン酸分解活性を30U/mlに調整し、1.5%Cellulase Onozuka R-10(Yakult本社)を添加したものを細胞壁分解酵素液とし、マコンブ芽胞体からプロトプラストの単離を試みた。15℃で反応を行った結果、約1時間後からプロトプラストの遊離が認められ、反応3時間後には藻体1g当たり4.5×10^6cells、5時間後には1.3×10^7cellsのプロトプラストが得られた。得られたプロトプラストは球形、黄金色で10〜25mumの大きさであった。また、ニュートラルレッド染色法によりほとんどの細胞が生存していることが確認された。さらにプロトプラストの収量と生存率を高める反応条件を検討した結果、プロトプラストの単離にはアルギン酸分解酵素の他にセルラーゼが必須であり、高張液の組成は海水濃度が50%、MgCl_2濃度は50〜100mM、緩衝液成分は5mMHEPES、マンニトールの濃度は0.5Mとした場合が至適であった。本条件下(MgCl_2濃度は25mM、反応3時間)で得られたプロトプラストの収量は5.2×10^6cells/g、生存率は98%であった。対照として市販のアワビアセトンパウダー(Sigma社)をアルギン酸分解酵素源とし、同一条件下で反応を行った場合ではプロトプラストの単離が認められず、Alteromonas sp.の産生するアルギン酸分解酵素の有効性が明らかとなった。また、マコンブプロトプラストの培養を試み、培養液への抗生物質の添加はプロトプラストの再生に悪影響を及ぼすことが明らかとなった。上記の至適培養条件下で単離したプロトプラストをProvasoliのESI培地で5℃、35001x、12h:12h明暗周期の条件下で培養を行ったところ、培養2〜3日目に細胞壁が再生し、6日目から分裂細胞が観察され始め、その後さらに繰り返し分裂した細胞も認められた。本結果からマコンブプロトプラスト培養系の確立が進展すると期待される。
文部科学省, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 05760144

水産無脊椎動物の腸内細菌に関する研究
Competitive research funding

海洋細菌の産生するアルギン酸分解酵素に関する研究
Competitive research funding

Studies on intestinal microflora of marine invertebrates
Competitive research funding

Studies on alginate degrading enzymes produced by marin bacteria.
Competitive research funding