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Seishi Hagihara
Field Science Center for Northern Biosphere　Aquatic Research Station　Nanae Fresh-Water Station
Associate Professor
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Last Updated :2024/05/09

Researcher Profile and Settings

Affiliation

  • Field Science Center for Northern Biosphere　Aquatic Research Station　Nanae Fresh-Water Station

Job Title

  • Associate Professor

URL

Research funding number

  • 80704501

J-Global ID

Research Interests

  • サケマス   人為催熟   性成熟   オオウナギ   熱帯ウナギ   春機発動   産卵   繁殖   sex differentiation   fish reproductive physiology   fish eco-physiology   freshwater eel, Anguilla   sturgeon, Acipenser, Huso   migration   

Research Areas

  • Life sciences / Marine/Aquatic life sciences
  • Life sciences / Molecular biology
  • Life sciences / Animals: biochemistry, physiology, behavioral science
  • Life sciences / Aquaculture

Academic & Professional Experience

  • 2023/03 - Today Hokkaido University
  • 2023/03 - Today Hokkaido University School of Fisheries Sciences Department of Aquaculture Life Science
  • 2023/03 - Today Hokkaido University Field Science Center for Northern Biosphere Aquatic Research Station
  • 2020/07 - 2023/02 The University of Tokyo Graduate School of Frontier Sciences
  • 2020/04 - 2023/02 The University of Tokyo Atmosphere and Ocean Research Institute
  • 2019/07 - 2020/03 The University of Tokyo Graduate School of Agricultural and Life Sciences
  • 2019/04 - 2019/07 The University of Tokyo Graduate School of Agricultural and Life Sciences
  • 2018/04 - 2019/03 Nihon University College of Bioresource Sciences
  • 2017/06 - 2018/03 Nihon University College of Bioresource Sciences
  • 2012/07 - 2017/03 Graduate School of Fisheries Sciences, Hokkaido University postdoctoral fellow
  • 2010/04 - 2012/03 The University of Tokyo
  • 2010/07 - 2010/09 中央水産研究所 契約職員 (乗船補助調査員)
  • 2008/10 - 2009/03 The University of Tokyo Graduate School of Agricultural and Life Sciences
  • 2008/10 - 2008/11 東北区水産研究所 非常勤職員 (乗船補助調査員)
  • 2008/04 - 2008/04 東北区水産研究所 非常勤職員 (乗船補助調査員)
  • 2007/10 - 2007/11 東北区水産研究所 非常勤職員 (乗船補助調査員)
  • 2007/06 - 2007/07 東北区水産研究所 非常勤職員 (乗船補助調査員)
  • 2007/04 - 2007/04 東北区水産研究所 非常勤職員 (乗船補助調査員)

Education

  • 2008/04 - 2012/06  The University of Tokyo  Graduate School of Agricultural and Life Sciences  Aquatic Bioscience
  • 2006/04 - 2008/03  The University of Tokyo  Graduate School of Frontier Sciences  Department of Natural Environmental Studies
  • 2004/04 - 2006/03  Tokyo University of Marine Science and Technology
  • 2002/04 - 2004/03  Tokyo University of Fisheries
  • 1999/04 - 2002/03  鹿児島県立川内高等学校

Association Memberships

  • THE JAPANESE SOCIETY OF FISHERIES SCIENCE   East Asia Eel Society   

Research Activities

Published Papers

  • Manabe R, Higuchi T, Watanabe S, Kaneko H, Miller MJ, Hagihara S, Yoshinaga T, Satou K, Aoyama J, Tsukamoto K
    Zoological Studies 62 46  2023 [Refereed][Not invited]
  • Nguyen AT, Dao HT, Quang HT, Hagihara S, Lokman PM, Damsteegt EL
    Fishes 7 (5) e286  2022/10 [Refereed][Not invited]
  • Hagihara S, Wakiya R, Maeda T, Kimura S
    Journal of Fish Biology 101 (3) 749 - 752 0022-1112 2022/09 [Refereed]
  • Horiuchi M, Hagihara S, Kume M, Chushi D, Hasegawa Y, Itakura H, Yamashita Y, Adachi S, Ijiri S
    Cells 11 (9) e1554  2022/05 [Refereed]
  • Hagihara S
    La mer 60 (1-2) 1 - 7 2022 [Refereed]
  • Hirase S, Kumai Y, Kato S, Hagihara S, Kikuchi K, Kuroki M
    Journal of Evolutionary Biology 35 (5) 763 - 771 1010-061X 2022 [Refereed]
  • M J Miller, S Wouthuyzen, J Aoyama, H Y Sugeha, S Watanabe, M Kuroki, A Syahailatua, S Suharti, S Hagihara, F Y Tantu, Trianto, T Otake, K Tsukamoto
    IOP Conference Series: Earth and Environmental Science 789 (1) e012011 - 012011 1755-1307 2021/06/01 [Refereed]
  • Hagihara Seishi (corresponding author), Aoyama J, Limbong D, Tsukamoto K
    Journal of Fish Biology 97 (6) 1842 - 1845 2020/12 [Refereed][Not invited]
  • Kuroki M, Miller MJ, Feunteun E, Sasal P, Pikering T, Han Y-S, Faliex E, Acou A, Dessier A, Schabetsberger R, Watanabe S, Kawakami T, Onda H, Higuchi T, Takeuchi A, Shimizu M, Hewavitarane CA, Hagihara S, Taka T, Kimura S, Mochioka N, Otake T, Tsukamoto K
    Progress in Oceanography 180 102234 - 102234 0079-6611 2020/01 [Refereed][Not invited]
  • Seishi Hagihara, Jun Aoyama, Ryusuke Sudo, Daniel Limbong, Shigeho Ijiri, Shinji Adachi, Katsumi Tsukamoto
    Journal of Fish Biology 96 (3) 558 - 569 0022-1112 2020 [Refereed][Not invited]
     
    © 2019 The Fisheries Society of the British Isles Downstream-migrating (n = 64) and non-migrating (n = 21) female Celebes eels Anguilla celebesensis were captured from the Poso Lake–River system on Sulawesi Island, Indonesia, and their reproductive physiological characteristics were examined. A histological observation of the ovaries revealed that most non-migrating eels were at the perinucleolus (43%) or oil-droplet (48%) stage, whereas most migrating eels were at the early vitellogenic (36%) or midvitellogenic (61%) stage. Transcript levels of gonadotropin genes (fshb, lhb) in the pituitary gland and concentrations of sex steroids [11-ketotestosterone (11-KT), testosterone, 17β-oestradiol (E2)] in blood plasma of migrating eels were significantly higher than those of non-migrating eels. The fshb messenger (m)RNA levels were lower in perinucleolus and oil-droplet stages and then significantly increased in the early vitellogenic stage. The lhb mRNA levels in vitellogenic-stage eels were significantly higher than those in perinucleolus- and oil-droplet-stage eels. The 11-KT levels of eels at the oil-droplet and vitellogenic stages were significantly higher than those of eels at the perinucleolus stage. The E2 levels at the vitellogenic stage were significantly higher than those at the perinucleolus and oil-droplet stages. These dynamics of the reproductive hormones represented the physiological background of oogenesis in A. celebesensis that has remarkably well-developed oocytes just before downstream migration.
  • Izumi H, Gen K, Lokman PM, Hagihara S, Horiuchi M, Tanaka T, Ijiri S, Adachi S
    Molecular Reproduction and Development 86 (12) 1846 - 1864 2019/12 [Refereed][Not invited]
  • Michael J. Miller, Sam Wouthuyzen, Eric Feunteun, Jun Aoyama, Shun Watanabe, Augy Syahailatua, Mari Kuroki, Tony Robinet, Seishi Hagihara, Tsuguo Otake, Katsumi Tsukamoto
    Deep-Sea Research Part II: Topical Studies in Oceanography 161 120 - 131 0967-0645 2019/03 [Refereed][Not invited]
     
    © 2018 Elsevier Ltd The unique semi-enclosed Indian Ocean basin includes large Mascarene Plateau banks, offshore coral-reef islands, seasonal equatorial current jets, and cross-basin westward South Equatorial Current (SEC) flow, making it interesting for studying long larval-duration eel larvae (leptocephali) and regional eel biodiversity. Three surveys for leptocephali (in 2003, 2006, 2010) included sampling west of the Mascarene Plateau (west), a major survey and other stations off Sumatra and Java (east), and 2 cross-basin transects across the SEC. The highest numbers of leptocephali species were observed along Sumatra (2003: ~143 species; 2006: 72 species) and south of Java (2010: 69), with intermediate numbers being collected in the western Indian Ocean (2006: 71; 2010: 53) compared to low numbers in the hydrographically variable offshore zones (2006, 2010: 3–27). The larger continental shelf areas along Sumatra including the Mentawai Islands provide more coral reef and other habitats for species such as congrid, muraenid, ophichthid, and chlopsid eels compared to the Mascarene Plateau banks. Some larvae in these areas get transported offshore, but the majority of offshore larvae were of Nemichthyidae and Serrivomeridae mesopelagic eels that were spawning across the basin. Habitat differences between the southern Mascarene Plateau and Sumatra and southern Indonesia along the edge of the high biodiversity Coral Triangle likely explain the higher biodiversity of eel larvae observed along the western side of the basin, which for the Congridae and Ophichthidae included more species than observed previously within the central Indonesian Seas. In addition to local spawning, seasonal currents likely transport larger larvae towards Sumatra from the north or west and larvae may enter the basin from the Indonesian Throughflow in the east, but it is unknown if equatorial jets or the SEC can transport larvae across the whole basin.
  • Seishi Hagihara, Jun Aoyama, Daniel Limbong, Katsumi Tsukamoto
    Journal of Fish Biology 93 (4) 729 - 732 0022-1112 2018/10 [Refereed][Not invited]
     
    © 2018 The Fisheries Society of the British Isles Downstream-migrating Anguilla celebesensis eels were predominant relative to Anguilla marmorata in October, November, December, January and February (75.9–92%), while no A. celebesensis occurred and A. marmorata were predominant in May and July (96–100%), at the outlet of Poso Lake, Sulawesi Island, Indonesia. Merging these results with those from published data suggests that most A. celebesensis start downstream migration during the early to middle rainy season, and A. marmorata migrate almost year-round with a peak from the late rainy to middle dry season.
  • Hagihara Seishi, Azuma N, Suyama K, Katakura S, Ijiri S, Adachi A
    Coastal Marine Science 41 7 - 10 1349-3000 2018/10 [Refereed][Not invited]
  • S. Hagihara, J. Aoyama, D. Limbong, K. Tsukamoto
    Journal of Fish Biology 92 (5) 1526 - 1544 1095-8649 2018/05/01 [Refereed][Not invited]
     
    The age and growth of migrating tropical eels, Anguilla celebesensis and Anguilla marmorata from central Sulawesi, Indonesia, were examined. Migrating eels (63 A. celebesensis and 38 A. marmorata) were obtained from weirs near the Poso Lake outlet and non-migrating eels (35 A. celebesensis and 119 A. marmorata) were captured by baited hooks, eel pots, scoop net and electro-fishing in the Poso River system, Laa River system, Baluga River, Tongku River and Padapu River from February 2009 to October 2010. In both species, the proportion of eels with opaque otolith edges showed a single peak in July, suggesting that one annulus (a pair of translucent and opaque zones) was formed each year in their otoliths. Mean ± s.d. and range of total length (LT) and age was 785·2 ± 114·9 (585–1083) mm and 7·5 ± 1·6 (5–11) years in migrating female A. celebesensis and 1132·2 ± 173·7 (800–1630) mm and 11·6 ± 3·3 (7–23) years in A. marmorata. The age of migrating female eels was negatively correlated with annual growth rate, 100·7 ± 17·2 (68·1–145·0) mm year−1 in A. celebesensis and 97·9 ± 19·3 (66·6–131·6) mm year−1 in A. marmorata, but there was no significant correlation between the LT and annual growth rate in either species. The annual growth rates of these female tropical eels were typically higher than those of temperate anguillid species, suggesting a latitudinal cline in growth rate in the genus Anguilla reflecting the environmental conditions of their growth habitat.
  • Hagihara Seishi (corresponding author), Aoyama J, Limbong D, Tsukamoto K
    Journal of Fish Biology 93 21 - 29 2018 [Refereed][Not invited]
  • Aoyama J, Wouthuyzen S, Miller M J, Sugeha H Y, Kuroki M, Watanabe S, Syahailatua A, Tantu F Y, Hagihara Seishi, Triyanto, Otake T, Tsukamoto K
    Zoological Studies 57 e30  2018 [Refereed][Not invited]
  • Hatsumi Okada, Seishi Hagihara, Katsumasa Yamashita, Shigeho Ijiri, Shinji Adachi
    AQUACULTURE 479 (1) 712 - 720 0044-8486 2017/10 [Refereed][Not invited]
     
    The mechanisms that govern sex differentiation in sturgeon are poorly understood. This study aimed to examine sexual dimorphic expression of foxl2 and dmrt1 in Amur sturgeon Acipenser schrenckii during molecular and morphological sex differentiation. Histologically, ovaries were first observed at 9 months after hatching (mah), reflected in an invaginated gonadal epithelium and the presence of germ cells just below the epithelium, whereas gonads with smooth epithelium and germ cells in the stroma were characterized as testis. Some fish showed undifferentiated gonads until 16 mah, but all sampled fish had morphologically differentiated sex by 19 mah. The full-length cDNAs of foxl2 and two types of dmrt1, dmrt1a and dmrt1b, of Amur sturgeon were isolated. The 2 types of dmrt1 cDNA had an identical structure from the 5'-UTR to the Y-rich domain, whereas the sequence downstream from this domain showed no homology and is considered to have resulted from alternative splicing. Foxl2 was principally expressed in the ovary and the 2 types of dmrt1 in the testis of 7-year-old Amur sturgeon, and much less so in various somatic tissues. Foxl2 expression was sexually dimorphic in morphologically differentiated gonads and was similarly dichotomous in the undifferentiated gonads of fish at 9 mah. In contrast, sexually dimorphic expression of dmrt1a and dmrt1b was not observed in gonads of the juvenile Amur sturgeon, but these genes showed testis-dominant expression in the adults. We suggest that foxl2 expression is closely related to ovarian differentiation and that two types of dmrt1 play a role in testis development and/or sperm formation rather than testicular differentiation. Additionally, we suggest that mRNA levels of foxl2 are an appropriate marker for sexing of sturgeon at an early developmental stage.
  • Milos Havelka, He Zhou, Seishi Hagihara, Masaki Ichimura, Takafumi Fujimoto, Etsuro Yamaha, Shinji Adachi, Katsutoshi Arai
    FISHERIES SCIENCE 83 (4) 587 - 595 0919-9268 2017/07 [Refereed][Not invited]
     
    We investigated the source of spontaneous polyploidization in the critically endangered Acipenser mikadoi. Fourteen sib progeny of A. mikadoi and 11 hybrids between an A. mikadoi female and a Huso dauricus male, all showing atypically high ploidy, were analysed. Parent assignment based on five highly polymorphic microsatellite markers confirmed spontaneous duplication of maternal chromosome sets via retention of the second polar body to be the source of spontaneous polyploidization. To our knowledge, this provides the first evidence of the maternal origin of spontaneous polyploidization in A. mikadoi. Factorial correspondence analysis of the multilocus microsatellite genotypes placed the parent fish of spontaneous polyploids in clearly delineated clusters of A. mikadoi and H. dauricus, and parent fish had mitochondrial control region haplotypes corresponding to their presumed species. Thus, parent fish were confirmed to be of pure genetic origin, and hybridization did not promote the observed spontaneous polyploidization.
  • Milos Havelka, Takafumi Fujimoto, Seishi Hagihara, Shinji Adachi, Katsutoshi Arai
    SCIENTIFIC REPORTS 7 1694  2045-2322 2017/05 [Refereed][Not invited]
     
    Sturgeons (Acipenseriformes) are among the most endangered species in the world due to fragmentation and destruction of their natural habitats and to overexploitation, mainly for highly priced caviar. This has led to the development of sturgeon culture, originally for reintroduction, but more recently for caviar production. In both cases, accurate species identification is essential. We report a new tool for accurate identification of Huso huso and Acipenser ruthenus based on nuclear DNA markers. We employed ddRAD sequencing to identify species- specific nucleotide variants, which served as specific binding sites for diagnostic primers. The primers allowed identification of Huso huso and Acipenser ruthenus as well as their discrimination from A. baerii, A. schrenckii, A. gueldenstaedtii, A. stellatus, A. persicus, A. mikadoi, A. transmontanus, and H. dauricus and identification of A. ruthenus and H. huso hybrids with these species, except hybrid between A. ruthenus and A. stellatus. The speciesspecific primers also allowed identification of bester (H. huso x A. ruthenus), the most commercially exploited sturgeon hybrid. The tool, based on simple PCR and gel electrophoresis, is rapid, inexpensive, and reproducible. It will contribute to conservation of remaining wild populations of A. ruthenus and H. huso, as well as to traceability of their products.
  • Shigeho Ijiri, Yasushi Shibata, Nonoha Takezawa, Yukinori Kazeto, Naoki Takatsuka, Erika Kato, Seishi Hagihara, Yuichi Ozaki, Shinji Adachi, Kohei Yamauchi, Yoshitaka Nagahama
    ENDOCRINOLOGY 158 (3) 627 - 639 0013-7227 2017/03 [Refereed][Not invited]
     
    The maturation-inducing hormone 17(alpha), 20(beta)-dihydroxy-4-pregnen-3-one (DHP) was first identified in the amago salmon. Although carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase (CR/20b-HSD) was reported to convert 17 alpha-hydroxyprogesterone (17OHP) to DHP in rainbow trout, we previously found that CR/20 beta-HSD messenger RNA (mRNA) was not upregulated in stimulated granulosa cells from masu salmon, which suggested that DHP is synthesized by a different enzyme. Accordingly, the current study aimed to identify the specific 20 beta-hydroxysteroid dehydrogenase (20 beta-HSD) responsible for DHP production by granulosa cells during final oocyte maturation in masu salmon. RNA sequencing was performed on granulosa layers that were isolated from ovarian follicles at 1 month before ovulation and incubated with or without forskolin, which was used to mimic luteinizing hormone, and similar to 12 million reads were obtained, which yielded 71,062 contigs of > 100 bp. tBlastx analysis identified 1 contig (#f103496)as similar to 17 beta-hydroxysteroid dehydrogenase type 12 (hsd17 beta 12); however, because the full-length #f103496 sequence was different from hsd17 beta 12, it was termed hsd17 beta 12-like (hsd17 beta 12l). We found that mammalian cells transfected with full-length hsd171 beta 2l exhibited considerable 20b-HSD activity, as indicated by efficient conversion of exogenous 17OHP to DHP. In addition, we found that hsd17 beta 12l mRNA levels were consistently low in follicles during vitellogenic growth; however, the levels increased significantly during final oocytematuration. The levels of hsd17 beta 12lmRNA were also considerably increased in granulosa layers in which 20 beta-HSD activity was induced by salmon pituitary extract. Therefore, we suggest that hsd17 beta 12l, not CR/20 beta-HSD, is the 20 beta-HSD responsible for DHP production by granulosa cells in masu salmon during final oocytematuration.
  • Noriko Azuma, Seishi Hagihara, Masaki Ichimura, Yasuaki Takagi, Kazuhiro Ura, Shinji Adachi
    ICHTHYOLOGICAL RESEARCH 64 (1) 139 - 144 1341-8998 2017/01 [Refereed][Not invited]
     
    Genetic characterization was performed in five individuals of wild Amur sturgeon Acipenser schrenckii, and/or its presumed hybrid caught around Hokkaido, using a mitochondrial DNA (mtDNA) marker and two markers of nuclear DNA (nDNA). Genetic analyses indicated that two of the five fish had the mtDNA haplotype of Kaluga, Huso dauricus, whereas the nDNA markers indicated signs for both A. schrenckii and H. dauricus genotypes, referring to a hybrid origin. The other three fish were plausibly pure A. schrenckii. The results indicated the importance of combined usage of mtDNA and nDNA markers for correct species identification in sturgeon.
  • Shun Watanabe, Seishi Hagihara, Michael J. Miller, Masamichi Machida, Kosei Komatsu, Shuhei Nishida, Katsumi Tsukamoto
    JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM 96 (8) 1701 - 1707 0025-3154 2016/12 [Refereed][Not invited]
     
    When and where marine eels spawn is poorly known even though species such as those of the family Congridae, Muraenidae and Ophichthidae can be caught in continental shelf habitats. The congrid genus Ariosoma includes small continental shelf eel species whose life histories are not yet known. Mature male and female eels of Ariosoma meeki were observed and captured on 17 August 2009 at the surface at night in the western side of the Kuroshio Current in the East China Sea close to new moon, while they were swimming slowly at the surface and exhibiting apparent reproduction- related behaviour. One male and one sex-unidentified eel (seemingly a male based on body shape) were observed to be chasing one larger female with their heads located near her urogenital pore area. The gonads of the female (540 mm) and the male (410 mm) that were caught by a long-handled dip net were in reproductive condition, because some eggs or seminal fluid were released during handling of the two specimens and high gonad-somatic index (GSI) values of 53 in the female and 20 in the male were recorded. This is one of the few cases in which fully ripe reproductive-condition marine eels have been observed or collected and it provides rare information about the spawning location and timing of this eel species.
  • Xi Zhang, Noriko Azuma, Seishi Hagihara, Shinji Adachi, Kazuhiro Ura, Yasuaki Takagi
    GENE 579 (1) 8 - 16 0378-1119 2016/03 [Refereed][Not invited]
     
    To characterize type I and II collagen in the Amur sturgeon at the molecular level, mRNAs encoding the pro alpha chain of both types of collagen were cloned and sequenced. Full sequences of both were obtained, and the molecular phylogeny based on the deduced amino acid sequence indicated that the correct sequences of the target genes were obtained. Analyses of primary structure of the pro alpha chains revealed that type I and II collagen share the basic structure of the prom chain of fibril collagen, but have different characteristics, especially in residues related to thermal stability. In the triple helical domain, Gly-Pro-Pro sequence stabilizing the tripeptide unit was more frequent in type II than in type I, and Gly-Gly, which likely decline in thermal stability, was more frequent in type I than in type II. These results suggested that the denaturation temperature of type II would be remarkably higher than type I. The spatial pattern of gene expression was analyzed by quantitative real-time PCR, which showed that relatively ubiquitous type I gene and strongly skewed distribution of type II gene, which highly expressed only in vertebra, snout cartilage, and notochord. This pattern was similar to the distribution pattern of each collagen protein detected by previous biochemical analyses using Amur and Bester sturgeons. The present study is the first report of the cloning of the full-length cDNAs for both of type I and type II collagen in the Amur sturgeon, and is the first comparative analysis of type I and II collagens in a sturgeon species at the molecular level. The results provide basic and general information on collagens in sturgeons. (C) 2015 Elsevier B.V. All rights reserved.
  • Maebayashi Mamoru, Hiramatsu Naoshi, Inaoka Yuhei, Yoshida Tatsuya, Hagihara Seishi, Nishimiya Osamu, Mushirobira Yuji, Adachi Shinji, Hara Akihiko, Todo Takashi
    Aquaculture Science 水産増殖談話会 64 (1) 63 - 76 0371-4217 2016 [Refereed][Not invited]
     
    Molecular cloning of cDNAs encoding an egg yolk precursor, vitellogenin (Vtg), was performed using the liver of estrogen-treated Amur sturgeon. Three kinds of vtg cDNA were cloned and temporarily named as vtg1, vtg2 and vtg3. These vtg cDNAs were obtained as contiguous sequences; each of vtg1, vtg2 and vtg3 sequences contained a complete open reading frame (5,307, 5,247 and 5,319 bp, respectively), each encoding 1,769, 1,749 and 1,773 aa residues, respectively. Similarity among amino acid sequences deduced from the three vtg cDNAs were relatively low, ranging from 64.3% to 47.9%. Three Vtgs appeared to be a complete-type, consisting of all representative yolk protein domains. Phylogenetic analysis revealed that Amur sturgeon Vtg1 formed a clade together with a published white sturgeon Vtg, while Amur sturgeon Vtg2 and Vtg3 formed another distinct clade. The results of phylogenetic analysis confirmed all three Vtgs belong to VtgAB type; vtg1/Vtg1, vtg2/Vtg2 and vtg3/Vtg3 were hereby designated as vtgAB1/VtgAB1, vtgAB2a/VtgAB2a and vtgAB2b/VtgAB2b, respectively. This study provided a basis to understand multiplicity in sturgeon vtg/Vtg and set a stage for their future application as reproductive biomarkers in sturgeon aquaculture.
  • Zuzana Linhartova, Taiju Saito, Vojtech Kaspar, Marek Rodina, Eva Praskova, Seishi Hagihara, Martin Psenicka
    THERIOGENOLOGY 84 (7) 1246 - 1255 0093-691X 2015/10 [Refereed][Not invited]
     
    Sturgeons (chondrostean, acipenseridae) are ancient fish species, widely known for their caviar. Nowadays, most of them are critically endangered. The sterlet (Acipenser ruthenus) is a common Eurasian sturgeon species with a small body size and the fastest reproductive cycle among sturgeons. Such species can be used as a host for surrogate production: application is of value for recovery of critically endangered and huge sturgeon species with an extremely long reproductive cycle. One prerequisite for production of the donor's gametes only is to have a sterile host. Commonly used sterilization techniques in fishes such as triploidization or hybridization do not guarantee sterility in sturgeon. Alternatively, sterilization can be achieved by using a temporary germ cell exclusion-specific gene by a knockdown agent, the antisense morpholino oligonucleotide (MO). The targeted gene for the MO is the dead end gene (drid) which is a vertebrate-specific gene encoding a RNA-binding protein which is crucial for migration and survival of primordial germ cells (PGCs). For this purpose, a dnd homologue of Russian sturgeon (Agdnd), resulting in the same sequence in the start codon region with isolated fragments of sterlet dnd (Ardnd), was used. Reverse transcription polymerase chain reaction confirmed tissue-specific expression of Ardnd only in the gonads of both sexes. Dnd-MO for depletion of PGCs together with fluorescein isothiocyanate (FITC)-biotin-dextran for PGCs labeling was injected into the vegetal region of one- to four-cell-stage sterlet embryos. In the control groups, only FITC was injected to validate the injection method and labeling of PGCs. After optimization of MO concentration together with volume injection, 250-mu M MO was applied for sterilization of sturgeon embryos. Primordial germ cells were detected under a fluorescent stereomicroscope in the genital ridge of the FITC-labeled control group only, whereas no PGCs were present in the body cavities of morphants at 21 days after fertilization. Moreover, the body cavities of MO-treated and nontreated fish were examined by histology and in situ hybridization, showing gonads which had no germ cells in morphants at various stages (60, 150, and 210 days after fertilization). Taken together, these results report the first known and functional method of sturgeon sterilization. (C) 2015 The Authors. Published by Elsevier Inc.
  • Ting Su, Shigeho Ijiri, Hirokazu Kanbara, Seishi Hagihara, De-Shou Wang, Shinji Adachi
    GENERAL AND COMPARATIVE ENDOCRINOLOGY 221 134 - 143 0016-6480 2015/09 [Refereed][Not invited]
     
    Estradiol-17 beta (E-2) and maturation-inducing hormone (MIH) are two steroid hormones produced in the teleost ovary that are required for vitellogenic growth and final oocyte maturation and ovulation. During this transition, the main steroid hormone produced in the ovary shifts from estrogens to progestogens. In the commercially important Japanese eel (Anguilla japonica), the MIH 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (DHP) is generated from its precursor by P450c17, which has both 17 alpha-hydroxylase and C17-20 lyase activities. In order to elucidate the regulatory mechanism underlying the steroidogenic shift from E-2 to DHP and the mechanistic basis for the failure of this shift in artificially matured eels, the cDNA for cypl7a2-which encodes P450c17-II-was isolated from the ovary of wild, mature Japanese eel and characterized, and the expression patterns of cyp17a1 and cyp17a2 during induced ovarian development were investigated in cultured eel ovaries. Five cDNAs (types I-V) encoding P450c17-II were identified that had minor sequence variations. HEK293T cells transfected with all but type II P450c17-II converted exogenous progesterone to 17 alpha-hydroxyprogesterone (17 alpha-P), providing evidence for 17 alpha-hydroxylase activity; however, a failure to convert 17 alpha-P to androstenedione indicated that C17-20 lyase activity was absent. Cyp17 alpha 2 mRNA was expressed mainly in the head kidney, ovary, and testis, and quantitative PCR analysis demonstrated that expression in the ovary increased during induced vitellogenesis and oocyte maturation/ovulation. In contrast, P450c17-I showed both 17a-hydroxylase and C17-20 lyase activities, and cyp17a1 expression increased until the mid-vitellogenic stage and remained high thereafter. Considering the high level of cyp17a2 transcript in the eel ovary at the migratory nucleus stage together with our previous report demonstrating that eel ovaries have strong 17 alpha-P-to-DHP conversion activity, the failure of artificially maturing eels to produce the maturationinducing DHP may be explained by a deficiency in 17 alpha-P production due to the persistence of cyp17a1 expression after the completion of vitellogenesis. (C) 2015 Elsevier Inc. All rights reserved.
  • Matsuya N, Hagihara Seishi (corresponding author), Wada T, Ijiri S, Adachi S
    Coastal Marine Science International Coastal Research Center, Atmosphere and Ocean Research Institute, the University of Tokyo 38 (1) 8 - 11 1349-3000 2015/07 [Refereed][Not invited]
     
    A female silver Japanese eel Anguilla japonica (681 mm TL) was captured by bottom trawl off Fukushima on 18 October 2013. This is the first report of an oceanic-migrating Japanese eel in the western North Pacific Ocean off northern Japan. We described the status of the silvering stage, morphological characteristics, age, developmental stage of the ovaries, and the serum levels of sex steroids for the eel. The GSI of the eel was 2.33 and the mean diameter of the largest group of oocytes was 269.9 μm and these were at the early vitellogenic stage. The serum E2 level was 3.9 ng/ml and 11-KT was 17.9 ng/ml. These steroid levels were higher than the mean steroid levels of female silver eels captured in Hamana Lake, but other morphological and physiological characteristics of the eel were not much different from those of silver eels captured in river, estuary, and coastal waters.
  • Hikari Izumi, Seishi Hagihara, Hiroaki Kurogi, Seinen Chow, Katsumi Tsukamoto, Hirohiko Kagawa, Hideaki Kudo, Shigeho Ijiri, Shinji Adachi
    FISHERIES SCIENCE 81 (2) 321 - 329 0919-9268 2015/03 [Refereed][Not invited]
     
    To describe the histological characteristics of the oocyte chorion in wild adult and artificially matured Japanese eels, we investigated changes in chorion thickness during artificially induced oogenesis and compared the chorion thickness and ultrastructure between wild and artificially matured eels. In artificially maturing eels, the chorion thickness and volume increased significantly with increasing follicle diameter, peaking at approximately 450 A mu m; beyond this point, the chorion thinned significantly, whereas there were no significant changes in volume. A significant positive correlation was observed between the number of salmon pituitary extract (SPE) injections and chorion thickness. In wild post-spawning adult eels, chorion thickness varied among individuals, and two had chorions that were significantly thinner than those of artificially matured eels. Ultrastructural examination revealed electron-dense layers in the chorions of wild post-spawning adult eels, as was seen in artificially matured eels. This result is inconsistent with our hypothesis that the formation of an electron-dense layer is unique to artificially maturing eels due to repeated SPE injections. These results suggest that the formation cycle of the chorion might be affected by SPE injections in artificially maturing eels, whereas that of wild eels might be synchronized with behavioral and/or environmental fluctuations that occur during the oceanic spawning migration.
  • S. Hagihara, R. Yamashita, S. Yamamoto, M. Ishihara, T. Abe, S. Ijiri, S. Adachi
    JOURNAL OF APPLIED ICHTHYOLOGY 30 (6) 1557 - 1564 0175-8659 2014/12 [Refereed][Not invited]
     
    The establishment of a sexing technique at an early developmental stage is an important issue in sturgeon aquaculture, yet the mechanism of sex differentiation in sturgeons remains poorly understood. This study aimed to identify genes involved in gonadal sex differentiation and to investigate sexually dimorphic gene expression in undifferentiated gonads. RNA-seq analyses using next-generation sequencers were carried out on undifferentiated gonads of five 9-month-old juvenile Russian sturgeon Acipenser gueldenstaedtii. A total of 45686832 (8498Mb) and 79743269 (7887Mb) quality-controlled reads were obtained using Ion PGM and HiSeq 2000 sequencers, respectively, and were assembled into 338648 contigs (N50: 532 b). tBLASTx analyses identified 26 transcripts potentially involved in gonadal differentiation. Read-mapping analyses were performed to obtain relative transcript levels (RPKM values). Multivariate analyses of the RPKM values of five transcripts (gsdf, dmrt1a, dmrt1b, foxl2 and cyp19a1a) showed that the five individuals could be separated into two distinct groups. One group, comprising two individuals, had increased levels of foxl2, hsd17b1 and cyp19a1a, suggesting differentiation into females. Alternatively, levels of gsdf were higher in the other three individuals, suggesting differentiation into males. We describe the levels of 26 transcripts potentially involved in gonadal differentiation in undifferentiated sturgeon gonads and suggest that levels of gsdf, foxl2, hsd17b1 and cyp19a1a are appropriate sexing markers for sturgeon at an early developmental stage.
  • Shun Watanabe, Jun Aoyama, Seishi Hagihara, Bunpei Ai, Rhodora V. Azanza, Katsumi Tsukamoto
    FISHERIES SCIENCE 79 (3) 375 - 383 0919-9268 2013/05 [Refereed][Not invited]
     
    Anguilla luzonensis and A. huangi were each described in 2009 using eels obtained from northern Luzon Island. We examined the taxonomic status of these two groups of eels using morphological and molecular genetic characters. There were no significant differences in two vertebrae counts between eels of A. luzonensis and A. huangi. Mitochondrial 16S ribosomal RNA and cytochrome b genes sequences were obtained and compared among 28 specimens of A. luzonensis, the holotypes of A. luzonensis and A. huangi, and one specimen of the other 15 anguillid species. The specimens of A. luzonensis exhibited almost identical sequences, including the holotype, with only a few site differences, and the genetic difference between the holotypes of A. luzonensis and A. huangi was within the range of differences of specimens of A. luzonensis. The other anguillid species were genetically very different from A. luzonensis and A. huangi, although A. interioris is a closely related species. It is clear that A. luzonensis and A. huangi are the same species, and according to the principle of priority in zoological nomenclature, A. luzonensis Watanabe, Aoyama, and Tsukamoto, 2009 is the valid species name, and A. huangi Teng, Lin, and Tzeng, 2009 is a junior synonym of A. luzonensis.
  • S. Hagihara, J. Aoyama, D. Limbong, K. Tsukamoto
    JOURNAL OF FISH BIOLOGY 81 (2) 408 - 426 0022-1112 2012/07 [Refereed][Not invited]
     
    The morphological and physiological characteristics of migrating and non-migrating female tropical eels, Anguilla celebesensis and Anguilla marmorata were examined in relation to their downstream migration on central Sulawesi Island, Indonesia. Migrating eels (64 A. celebesensis and 37 A. marmorata) were obtained from weirs set near the outlet area of Poso Lake and non-migrating eels (21 A. celebesensis and 21 A. marmorata) were sampled by set-lines and eel pots in Poso Lake, its inlet rivers, and in the La River system during February 2009 to October 2010. In both species, values of eye index, pectoral-fin length index, gonado-somatic index (IG), hepato-somatic index, swimbladder-somatic index and cardio-somatic index of migrating eels were significantly higher than those of non-migrating eels and the gut-somatic index values of the migrating eels were significantly lower than that of non-migrating eels. When silvering stages of eels were classified by the silvering index for Anguilla japonica, in A. celebesensis, all non-migrating eels were Y1 stage and the migrating eels consisted of Y2, S1 and S2 stages eels. In A. marmorata, the non-migrating eels consisted of Y1 and Y2 eels, and the migrating eels consisted of Y2 and S1 eels, but there were no S2 eels. Results of principal component analysis (PCA) of morphological and physiological variables suggested that these characteristics changed drastically between the Y1 and Y2 stages in A. celebesensis, while A. marmorata showed a gradual change with silvering, which differs from the temperate species A. japonica. The mean +/- s.d. IG value of migrating A. celebesensis (6.9 +/- 1.8, 3.311.4) was very high and that of A. marmorata (3.1 +/- 0.8, 1.85.7) was comparatively low. The very different rates of maturation that were found between these two species provide support for the hypothesis that the reproductive characteristics of silver eels can reflect their migration scale.
  • Shun Watanabe, Midori Iida, Seishi Hagihara, Hiromitsu Endo, Keiichi Matsuura, Katsumi Tsukamoto
    CYBIUM 35 (4) 371 - 379 0399-0974 2011/12 [Refereed][Not invited]
     
    The oceanic early life of Sicydiinae gobies has remained mysterious although considerable knowledge has been accumulated about their freshwater life. Two marine post-larvae of Sicyopterus japonicus. were collected by otter trawl in the ocean off Shikoku. Japan on 18 July and 22 August 2007. These specimens were identified as S. japonicus using meristic characters and the 16S ribosomal RNA mitochondrial gene, which were compared to S. japonicus adults and two other species of Sicydiinae gobies. Morphological observations documented that S. japonicus post-larval characters were the presence of a red pigment at the bases of the second dorsal and caudal fins in fresh samples, as well as the presence of melanophores on the upper jaw, caudal fin base and the lateral body posterior to the anal fin base. Otolith analysis showed that their post-larval durations were 278 and 286 days, and their birth dates were 13 October and 9 November 2006. It is likely that these two post-larvae may have been transported by the Kuroshio Current from the southern part of their geographic species range such as from Okinawa or Taiwan and may have had recruited to a river around Kochi.
  • Tatsuki Yoshinaga, Michael J. Miller, Kazuki Yokouchi, Tsuguo Otake, Shingo Kimura, Jun Aoyama, Shun Watanabe, Akira Shinoda, Machiko Oya, Sachie Miyazaki, Kei Zenimoto, Ryusuke Sudo, Tetsuya Takahashi, Hyojin Ahn, Ryotaro Manabe, Seishi Hagihara, Hiroshi Morioka, Hikaru Itakura, Masamichi Machida, Kazuyuki Ban, Mayu Shiozaki, Bunpei Ai, Katsumi Tsukamoto
    FISHERIES SCIENCE 77 (6) 983 - 992 0919-9268 2011/11 [Refereed][Not invited]
     
    Eggs of the Japanese eel Anguilla japonica collected in the western North Pacific were identified by onboard species-specific polymerase chain reaction (PCR) and DNA nucleotide sequencing after the cruise. Fish eggs of various species were collected by large plankton net tows at 12 stations along the southern part of the West Mariana Ridge on 19-25 May 2009. A total of 43 fish eggs were distinguished morphologically as possibly being of A. japonica. Thirty-one of those were analyzed by PCR, which included 15 eggs collected at 12 degrees 50-55'N, 141 degrees 15-20'E (in 5 tows) that showed positive results. The 16S ribosomal RNA (rRNA) gene sequences of eggs determined after the cruise indicated that 31 A. japonica eggs had been collected. The remaining eggs were of mesopelagic eel species (Serrivomeridae and Derichthyidae), or unidentified species. The morphological characteristics of the A. japonica eggs were consistent with those of artificially spawned eggs, except they had a slightly larger diameter. The egg diameter range did not overlap with those of mesopelagic eels of the Serrivomeridae, which often spawn in the same area as A. japonica. These results suggest that egg diameter and embryo shape can be used to morphologically identify naturally spawned A. japonica eggs.
  • Akira Shinoda, Jun Aoyama, Michael J. Miller, Tsuguo Otake, Noritaka Mochioka, Shun Watanabe, Yuki Minegishi, Mari Kuroki, Tatsuki Yoshinaga, Kazuki Yokouchi, Nobuto Fukuda, Ryusuke Sudo, Seishi Hagihara, Kei Zenimoto, Yuzuru Suzuki, Machiko Oya, Tadashi Inagaki, Shingo Kimura, Atsushi Fukui, Tae Won Lee, Katsumi Tsukamoto
    REVIEWS IN FISH BIOLOGY AND FISHERIES 21 (3) 591 - 611 0960-3166 2011/09 [Refereed][Not invited]
     
    The distribution of all larval stages of the Japanese eel, Anguilla japonica, were examined using historical catch records and original data in the western North Pacific (WNP) to evaluate existing information about the larval distribution and migration of this species. A total of 148 preleptocephali, 2547 leptocephali, 6 metamorphosing larvae, and 21 glass eels were collected during 37 cruises over a 52-year period (1956-2007). Sampling effort was spatio-temporally biased in latitude/longitude among seasons with sampling effort being concentrated near the western margin of the subtropical gyre near Taiwan in the winter season and extensive effort occurring near the spawning area to the east near the seamount chain of the West Mariana Ridge in summer during the spawning season. The distribution of preleptocephali (4.2-8.7 mm) was limited to a narrow area around 14A degrees N, 142A degrees E just west of the southern part of the seamount chain, while leptocephali (7.7-62.0 mm) were widely distributed at increasing size westward in the North Equatorial Current (NEC) to the region east of Taiwan. Metamorphosing larvae (52.7-61.2 mm) were collected only in the area 21-26A degrees N, 121-129A degrees E to the east of Taiwan, while glass eels (51.3-61.2 mm) occurred only within or west of the Kuroshio. These distributions suggest that leptocephali begin to metamorphose within or just east of the Kuroshio, then after completion of metamorphosis the glass eels detrain from the current and migrate inshore. The relationship between catch date and body size of leptocephali suggested that the spawning season is from April to August, but further sampling is needed to eliminate possible effects of sampling bias. This analysis is consistent with the existing hypothesis that Japanese eel larvae born near the West Mariana Ridge are transported westward in the NEC and then transfer to the Kuroshio to recruit to East Asia, although more sampling effort is needed for later stage larvae in the NEC bifurcation region to help understand the larval migration in relation to the possible impacts of ocean-atmosphere changes.
  • Shigeaki Kojima, Reina Maeda, Kei Sakuma, Yutaka Kokubu, Seishi Hagihara, Masaki Itoh
    Plankton and Benthos Research 6 (2) 108 - 114 1880-8247 2011 [Refereed][Not invited]
     
    We determined the nucleotide sequences of the 3' region (~400 base pairs) of the mitochondrial control region for 97 individuals of the deep-sea demersal fish species Bothrocara hollandi, which were collected at five sites in the northwestern Pacific off Tohoku District, the northeastern part of the Japanese mainland. Phylogenetic analysis based on the sequences showed that these fish form a monophyletic group with individuals of the Okhotsk Sea, which have completely deviated from those fish of the Japan Sea. Furthermore, genetic diversity of fish in the northwestern Pacific was higher than that in the Okhotsk Sea. The population of the northwestern Pacific was shown to have experienced a recent population expansion. Nine of 97 individuals had only one non-coding unit, and the remaining individuals had two units between mitochondrial genes for tRNA Thr and tRNA Pro however, neither of these groups of individuals formed a monophyletic group in the Okhotsk Sea or the northwestern Pacific, while monophyly of individuals with more than one unit was shown in the Japan Sea. The differences between the populations of the Japan Sea and neighboring sea areas might be attributed to the occurrence of repeated environmental changes and corresponding population bottleneck events in the Japan Sea. © The Japanese Association of Benthology.
  • Takero Adachi, Seishi Hagihara, Masaki Itoh, Gento Shinohara, Ikuo Hayashi, Shigeaki Kojima
    ICHTHYOLOGICAL RESEARCH 56 (4) 323 - 329 1341-8998 2009/11 [Refereed][Not invited]
     
    The genetic population structure and the diagnostic characters of Malacocottus gibber from the Japan Sea and Malacocottus zonurus from the Okhotsk Sea and the northwestern Pacific were compared. Analysis of the nucleotide sequences of the mitochondrial control region revealed no genetic differences between the populations of M. gibber and M. zonurus, even though most individuals of both the species were found to be morphologically distinct. Most of the Malacocottus gibber specimens had the typical morphological characters of this species, namely the absence of an accessory spine on the preopercle of both sides and the absence of modified body scales above the lateral line. All the specimens of M. zonurus had accessory spines on both sides, and most of them had modified body scales. The results of this study suggest that M. gibber should be treated as a subspecies or a synonym of M. zonurus. The nested clade analysis and the analysis of molecular variance (AMOVA) showed that the Japanese Malacocottus fishes are genetically homogenous over their geographical range. The mismatch distribution of the Japanese Malacocottus fishes indicated that a sudden population expansion had occurred recently. The contrast in phylogeographic structures between the Malacocottus fish and the zoarcid Bothrocara hollandi-the most dominant deep-sea demersal fish in the Japan Sea-might be attributed to the differences in the depths of the habitats and larval ecology between these two fishes.
  • M. Kuroki, J. Aoyama, M. J. Miller, T. Yoshinaga, A. Shinoda, S. Hagihara, K. Tsukamoto
    JOURNAL OF FISH BIOLOGY 74 (9) 1853 - 1865 0022-1112 2009/06 [Refereed][Not invited]
     
    Extensive collections were made of the larvae of the temperate Japanese eel Anguilla japonica and the tropical giant mottled eel Anguilla marmorata in an overlapping area of the North Equatorial Current region of the western North Pacific Ocean. Collections of 189 A. marmorata and > 2500 A. japonica larvae during nine surveys from 1991 to 2007 showed that these two anguillid eels have similar spawning areas just west of the southern West Mariana Ridge. In July to August 2006 and August 2007, morphologically and genetically identified A. marmorata preleptocephali were mainly collected between 14.5-15 degrees N and 142-142.5 degrees E, where A. japonica preleptocephali were also caught in some of the same net tows. Fewer A. marmorata preleptocephali, however, were collected (n = 31) compared to those of A. japonica (n = c. 165), and fewer small larvae of A. marmorata were collected per tow than A. japonica (n = 1-10 and 1-294, respectively), suggesting relatively smaller spawning aggregations of A. marmorata. The distribution of preleptocephali and small larvae was wider in longitude in A. marmorata (131- 143 degrees E) than in A. japonica (137-143 degrees E), while the latitudinal range was almost the same (12-17 degrees N). Although spawning by these two species overlaps both spatially and temporally, the tropical eels of the North Pacific population of A. marmorata probably have a much longer spawning season with fewer spawners, at least in summer, and recruit to a much wider latitudinal range of growth habitats.

Books etc

  • Eel Science
    Hagihara S, Sudo R (Contributor13. Metamorphosis and Silvering)
    Springer (Fisheries Science Series) 2023/10
  • ウナギの科学 (シリーズ 水産の科学)
    萩原聖士 (Contributor3.13. 銀化変態)
    朝倉書店 2019/06

Conference Activities & Talks

  • 淡水養成されたサクラマスの成長モニタリング：カメラ計測とPITタグによる成長追跡  [Not invited]
    山崎祐人, 松尾周佳, 池上温史, 高橋英佑, 萩原聖士, 高橋勇樹, 米山和良
    令和6年度日本水産学会春季大会  2024/03  東京, 港区
  • 養殖ニホンウナギの放流直後の生残と移動  [Not invited]
    竹内宏太, 脇谷量子郎, 前田達彦, 板倉光, 萩原聖士, 安樂和彦, 小谷知也, 木村伸吾
    令和6年度日本水産学会春季大会  2024/03  東京, 港区
  • 放流したニホンウナギの生残率の推定  [Not invited]
    竹内宏太, 脇谷量子郎, 前田達彦, 板倉光, 萩原聖士, 安樂和彦, 小谷知也, 木村伸吾
    東アジア鰻学会 第７回研究発表会  2024/03  東京, 文京区
  • Short-term apparent survival rate of farmed-and-stocked Japanese eels  [Not invited]
    Takeuchi K, Wakiya R, Maeda T, Hagihara S, Itakura H, Anraku K, Kotani, Kimura S
    9th World Fisheries Congress  2024/03  Seattle, Washington
  • ニホンウナギおよびオオウナギの河川における回帰性  [Not invited]
    前田達彦, 萩原聖士, 竹内宏太, 脇谷量子郎, 板倉光, 木村伸吾
    令和5年度日本水産学会春季大会  2023/03
  • 放流した養殖ニホンウナギの放流後一年間の生残と移動  [Not invited]
    竹内宏太, 脇谷量子郎, 前田達彦, 萩原聖士, 板倉光, 木村伸吾
    令和5年度日本水産学会春季大会  2023/03
  • オオウナギの移送放流後の回帰行動および血中コルチゾル濃度  [Not invited]
    前田達彦, 萩原聖士, 脇谷量子郎, 板倉光, 木村伸吾
    東アジア鰻学会第6回研究発表会  2023/03
  • 放流した養殖ニホンウナギの生態学的特徴と放流時の栄養状態との関係  [Not invited]
    竹内宏太, 前田達彦, 脇谷量子郎, 萩原聖士, 板倉光, 木村伸吾
    東アジア鰻学会第6回研究発表会  2023/03
  • 養殖ニホンウナギの河川放流後の生残と移動  [Not invited]
    竹内宏太, 脇谷量子郎, 前田達彦, 萩原聖士, 板倉光, 木村伸吾
    日本動物学会 第93回 早稲田大会  2022/09
  • ウナギの銀化と降海回遊  [Invited]
    萩原聖士
    東アジア鰻学会公開シンポジウム「うな丼の未来9 ~川のウナギの生物学~」  2022/07
  • 放流ニホンウナギの移動・分布の特性  [Not invited]
    竹内宏太, 前田達彦, 脇谷量子郎, 萩原聖士, 木村伸吾
    東アジア鰻学会 第5回研究発表会  2022/03
  • 河川におけるウナギ属魚類の定住性と回帰性  [Not invited]
    前田達彦, 竹内宏太, 脇谷量子郎, 萩原聖士, 木村伸吾
    東アジア鰻学会 第5回研究発表会  2022/03
  • 養殖ニホンウナギの放流後の移動分散特性  [Not invited]
    竹内宏太, 前田達彦, 脇谷量子郎, 萩原聖士, 木村伸吾
    令和4年度 日本水産学会春季大会  2022/03
  • ウナギ属魚類の定住性検証のための野外実験  [Not invited]
    前田達彦, 竹内宏太, 脇谷量子郎, 萩原聖士, 木村伸吾
    令和4年度 日本水産学会春季大会  2022/03
  • 北西太平洋におけるオオウナギの幼生分散過程  [Not invited]
    祝倩怡, 熊觀梅, 萩原聖士, 脇谷量子郎, 羽根由里奈, 板倉 光, 木村伸吾
    令和4年度 日本水産学会春季大会  2022/03
  • Spawning migration behavior of Anguilla celebesensis  [Not invited]
    Watanabe S, Higuchi T, Manabe R, Miller MJ, Hagihara S, Tsukamoto K
    World Fisheries Congress  2021/09
  • 初夏までに春機発動したウナギが秋に銀化する  [Not invited]
    萩原聖士, 板倉光, 長谷川祐也, 井尻成保, 足立伸次, 塚本勝巳
    東アジア鰻学会 第4回研究発表会  2021/03
  • 黄ウナギと銀ウナギの脳外部形態の比較  [Not invited]
    渡邉茂, 篠塚一貴, 山本直之, 萩尾華子, 萩原聖士
    東アジア鰻学会 第4回研究発表会  2021/03
  • ニホンウナギの遊泳行動:実験室及び半自然状態での解析  [Not invited]
    篠塚一貴, 渡邊俊, 萩原聖士, 坪川達也, 三田村啓理, 市川光太郎, 荒井修亮, 塚本勝巳, 渡邉茂
    東アジア鰻学会 第4回研究発表会  2021/03
  • 耳石安定同位体比を用いた二ホンウナギ放流個体の成長と銀化に関する研究  [Not invited]
    吉田拓未, 萩原聖士, 脇谷量子郎, 須藤竜介, 白井厚太郎, 三宅陽一, 木村伸吾
    東アジア鰻学会 第4回研究発表会  2021/03
  • ウナギの春機発動・銀化・降海回遊  [Invited]
    萩原聖士
    第1回ウナギ学の現状(仮)  2021/03
  • ウナギとチョウザメと人  [Invited]
    萩原聖士
    世田谷区生涯大学  2020/11
  • 熱帯ウナギの降海回遊生態と、若手研究者の育児生態 (若手の会講演会)  [Invited]
    萩原聖士
    令和元年度日本水産学会北海道支部大会  2019/11
  • 単独飼育個体を用いた養成ニホンウナギの形態的性分化直後の雌雄生殖腺における性分化関連遺伝子の探索 (最優秀講演賞)  [Not invited]
    堀内萌未, 稲葉駿, 萩原聖士, 井尻成保, 足立伸次
    令和元年度日本水産学会北海道支部大会  2019/11
  • ウナギの性成熟  [Invited]
    萩原聖士
    大森淡水グループ第40回鰻供養祭記念シンポジウム「宮崎発ウナギ研究最前線 ”ウナギの今”」  2019/10
  • Swimming pattern of Japanese eels (Anguilla japonica) in laboratory and semi-natural settings  [Not invited]
    Shinozuka K, Watanabe S, Hagihara S, Mitamura H, Ichikawa K, Arai N, Tsukamoto K, Watanabe S
    日本動物心理学会第79回大会  2019/10
  • セレベスウナギAnguilla celebesensisの産卵回遊行動  [Not invited]
    渡邊 俊, 樋口貴俊, 眞鍋諒太朗, 萩原聖士, 塚本勝巳
    令和元年度日本水産学会秋季大会  2019/09
  • 天然ニホンウナギの生殖腺の性分化  [Not invited]
    堀内萌未, 中司大智, 長谷川祐也, 萩原聖士, 板倉 光, 山下 洋, 久米 学, 寺島佑樹, 塚本勝巳, 井尻成保, 足立伸次
    東アジア鰻学会 第2回研究発表会  2019/03
  • 天然ニホンウナギにおける形態的性分化開始時期  [Not invited]
    堀内萌未, 中司大智, 長谷川祐也, 萩原聖士, 板倉 光, 山下 洋, 久米 学, 寺島佑樹, 塚本勝巳, 井尻成保, 足立伸次
    平成31年度日本水産学会春季大会  2019/03
  • 熱帯ウナギの降海回遊生態  [Not invited]
    HAGIHARA Seishi
    プロジェクト「鰻脳」キックオフ・シンポジウム 大規模回遊の神経機構解明を目指して  2018/06
  • 熱帯ウナギの降海回遊生態 (ポスター発表)  [Not invited]
    萩原聖士
    東日本魚類生態研究会  2018/02
  • チョウザメという生き物―古代魚と人間と美深と― (市民講演)  [Invited]
    萩原聖士
    チョウザメ事業推進講演会「びふかのチョウザメの今と未来」  2017/03
  • チョウザメ科種間雑種の発生/孵化および仔魚の生残における雑種強勢の可能性 (最優秀講演賞)  [Not invited]
    萩原聖士, 山中智樹, 須山喜市, 橋本富夫, 井尻成保, 足立伸次
    平成28年度日本水産学会北海道東北支部合同大会  2016/10
  • アムールチョウザメの形態的未分化生殖腺における新規性分化関連遺伝子の探索  [Not invited]
    須山喜市, 松原薫子, 萩原聖士, 石野魁盛, 後藤直英, 山本真也, 井尻成保, 足立伸次
    平成28年度日本水産学会北海道東北支部合同大会  2016/10
  • 北海道に来遊したアムールチョウザメ野生魚の遺伝的特徴  [Not invited]
    東 典子, 萩原聖士, 都木靖彰, 浦 和寛, 足立伸次, 市村政樹
    平成28年度日本水産学会秋季大会  2016/09
  • Spontaneous polyploidy in Acipenser mikadoi  [Not invited]
    Havelka M, Zhou H, Ichimura M, Hagihara Seishi, Fujimoto T, Yamaha E, Adachi S, Arai K
    平成28年度日本水産学会秋季大会  2016/09
  • Transcriptional profiling of olfactory imprinting in the olfactory center of Pacific salmon (poster presentation)  [Not invited]
    MinowaY, Hagihara S, Abe T, Ijiri S, Adachi S, Kudo H
    International Symposium on Olfaction and Taste  2016/06
  • 東シナ海における繁殖直前のゴテンアナゴAriosoma meekiの雌雄親魚の採集報告  [Not invited]
    渡邊俊, 萩原聖士, Miller J. Michael, 町田真道, 小松幸生, 西田周平, 塚本勝巳
    平成28年度日本水産学会春季大会  2016/03
  • 次世代シーケンサを用いたアムールチョウザメの未分化生殖腺における網羅的発現解析  [Not invited]
    松原薫子, 石野魁盛, 萩原聖士, 後藤直英, 山本真也, 井尻成保, 足立伸次
    平成28年度日本水産学会春季大会  2016/03
  • 北海道沿岸における2015年のチョウザメ科魚類の異常漁獲および生殖腺の特徴  [Not invited]
    萩原聖士, 須山喜市, 東典子, 堤尚信, 西尾朋高, 市村政樹, 三坂尚行, 鈴木渉太, 宮城大助, 古市明文, 市川卓, 松原創, 川崎琢真, 宗原弘幸, 高橋英祐, 山羽悦郎, 井尻成保, 足立伸次
    平成28年度日本水産学会春季大会  2016/03
  • 未熟ウニ生殖巣の肥大に伴う核内受容体の発現変化  [Not invited]
    佐藤卓史, 西宮攻, 萩原聖士, 井尻成保, 足立伸次, 浦和寛, 都木靖彰
    平成28年度日本水産学会春季大会  2016/03
  • 人為催熟ニホンウナギ卵の母性mRNA量と局在  [Not invited]
    泉ひかり, 堀内萌未, 玄浩一郎, 萩原聖士, 井尻成保, 足立伸次
    平成28年度日本水産学会春季大会  2016/03
  • サケ前脳における母川想起関連分子の探索  [Not invited]
    箕輪ゆい, 萩原聖士, 井尻成保, 工藤秀明, 足立伸次
    第9回サケ学研究会  2015/12
  • Sterilization of Sterlet Acipenser ruthenus by using knock down agent, antisense morpholino oligonucleotide, against dead end gene  [Not invited]
    Linhartová Z, Saito T, Kašpar V, Rodina M, Prašková E, Hagihara Seishi, Pšenička M
    5th International Workshop on the Biology of Fish Gametes  2015/09
  • チョウザメ雑種2倍体および3倍体の妊性推定  [Not invited]
    高柳耀, 萩原聖士, 宮本真先, 藤本貴史, 井尻成保, 山羽悦郎, 足立伸次, 荒井克俊
    平成27年度日本水産学会春季大会  2015/03
  • ニホンウナギにおける卵質に関わる母性mRNA群の探索  [Not invited]
    泉ひかり, 萩原聖士, 松谷紀明, 田中寿臣, 玄浩一郎, 井尻成保, 足立伸次
    平成27年度日本水産学会春季大会  2015/03
  • 天然ニホンウナギの精巣発達に伴うgsdf 免疫陽性細胞の局在変化  [Not invited]
    陳天燕, 松谷紀明, 宮川寛貴, 泉ひかり, 萩原聖士, 井尻成保, 足立伸次
    平成26年度日本水産学会北海道支部大会  2014/12
  • アムールチョウザメ卵母細胞の第 2 次成長開始マーカー遺伝子の探索  [Not invited]
    鈴木俊彦, 萩原聖士, 石原学, 井尻成保, 足立伸次
    平成26年度日本水産学会北海道支部大会  2014/12
  • ロシアチョウザメの形態的未分化生殖腺におけるgsdfの発現  [Not invited]
    後藤直英, 山下量平, 石野魁盛, 山本真也, 萩原聖士, 井尻成保, 足立伸次
    平成26年度日本水産学会北海道支部大会  2014/12
  • ウニ生殖巣の肥大に関与する核内受容体の発現解析  [Not invited]
    佐藤卓史, 相澤俊介, Heng Wang, 萩原聖士, 東典子, 井尻成保, 足立伸次, 浦和寛, 都木靖彰
    平成26年度日本水産学会秋季大会  2014/09
  • 萩原聖士, 石原学, 山下量平, 鈴木俊彦, 井尻成保, 足立伸次
    平成26年度日本水産学会秋季大会  2014/09
  • 斎藤大樹, Zuzana Linhartova, 萩原聖士, 井尻成保, 足立伸次, 山羽悦郎, 藤本貴史, 荒井克俊, Martin Psenicka
    平成26年度日本水産学会秋季大会  2014/09
  • 宮川寛貴, 松谷紀明, 青島秀治, 田中寿臣, 山内悟, 萩原聖士, 井尻成保, 足立伸次
    平成26年度日本水産学会秋季大会  2014/09
  • ロシアチョウザメの形態的未分化生殖腺における性分化関連遺伝子の発現 (ポスター発表)  [Not invited]
    萩原聖士
    函館研究交流会  2014/09
  • 萩原聖士
    函館研究交流会  2014/09
  • 石野魁盛, 萩原聖士, 山下量平, 山本真也, 後藤直英, 井尻成保, 足立伸次
    日本動物学会北海道支部 第59回大会  2014/08
  • 水品亜由菜, 井尻成保, 泉ひかり, 松谷紀明, 萩原聖士, 工藤秀明, 足立伸次, 張成年, 黒木洋明
    日本動物学会北海道支部 第59回大会  2014/08
  • 萩原聖士
    公開シンポジウム うな丼の未来Ⅱ人とうなぎの共存をめざして  2014/07
  • Expression analysis of sex differentiation-related genes in morphologically undifferentiated gonads of Russian sturgeon, and challenge to identify female-specific DNA marker (poster presentation)  [Not invited]
    Hagihara Seishi, Yamashita R, Yamamoto S, Goto N, Ijiri S, Adachi S
    Hokkaido-Singapore Symposium on Food Science and Biotechnology 2014  2014/06
  • Screening of candidate genes related to egg quality in Japanese eel (poster presentation)  [Not invited]
    Izumi H, Hagihara Seishi, Tanaka T, Gen K, Ijiri S, Adachi S
    Hokkaido-Singapore Symposium on Food Science and Biotechnology 2014  2014/06
  • The mRNA expression analysis of sex differentiation-related genes in the gonads of wild and cultured Japanese eel Anguilla japonica  [Not invited]
    Matsuya N, Miyakawa H, Aoshima S, Yamauchi S, Tanaka T, Wakiya R, Harima E, Mochioka N, Izumi H, Hagihara Seishi, Ijiri S, Adachi S
    Hokkaido-Singapore Symposium on Food Science and Biotechnology 2014  2014/06
  • ウニ生殖巣における主要卵黄タンパク質の発現機構解析  [Not invited]
    浦和寛, 佐藤卓史, Heng Wang, 萩原聖士, 東典子, 井尻成保, 足立伸次, 都木靖彰
    平成26年度日本水産学会春季大会  2014/03
  • ニホンウナギ天然雌親魚の排卵後卵巣の組織学的特徴 (ポスター発表)  [Not invited]
    水品亜由菜, 井尻成保, 泉ひかり, 松谷紀明, 萩原聖士, 工藤秀明, 足立伸次, 張成年, 黒木洋明
    平成26年度日本水産学会春季大会  2014/03
  • ニホンウナギ卵における卵質関連候補遺伝子群 RNA の発現量解析 (ポスター発表)  [Not invited]
    泉ひかり, 萩原聖士, 田中寿臣, 玄浩一郎, 井尻成保, 足立伸次
    平成26年度日本水産学会春季大会  2014/03
  • gsdf 抗体を用いたチョウザメ類の未分化生殖腺の免疫組織化学的観察 (ポスター発表)  [Not invited]
    山本真也, 後藤直英, 萩原聖士, 井尻成保, 足立伸次
    平成26年度日本水産学会春季大会  2014/03
  • ニホンウナギの生殖腺における性分化関連遺伝子の発現解析  [Not invited]
    松谷紀明, 宮川寛貴, 青島秀治, 山内悟, 田中寿臣, 脇谷量子郎, 播磨英三朗, 望岡典隆, 泉ひかり, 萩原聖士, 井尻成保, 足立伸次
    平成26年度日本水産学会春季大会  2014/03
  • 次世代シーケンサーを用いたロシアチョウザメの未分化生殖腺における性分化関連遺伝子の発現解析  [Not invited]
    萩原聖士, 山下量平, 山本真也, 後藤直英, 井尻成保, 足立伸次
    平成26年度日本水産学会春季大会  2014/03
  • Acipenser medirostrisとされている北日本に来遊するチョウザメ属魚類はA. mikadoiである  [Not invited]
    萩原聖士, 東典子, 井尻成保, 足立伸次
    平成26年度日本水産学会春季大会  2014/03
  • 浜名湖産天然ニホンウナギの形態的性分化過程 (最優秀学生講演賞)  [Not invited]
    松谷紀明, 宮川寛貴, 青島秀治, 山内悟, 田中寿臣, 泉ひかり, 萩原聖士, 井尻成保, 足立伸次
    平成 25 年度日本水産学会北海道支部大会  2013/12
  • Dimorphic expression pattern of sex differentiation-related genes in morphologically undifferentiated gonads of Amur sturgeon and Russian sturgeon (Invited lecture)  [Not invited]
    Ijiri S, Hagihara Seishi, Yamashita R, Suzuki H, Adachi S
    Diversification in Inland Finfish Aquaculture II  2013/09
  • ロシアチョウザメの未分化生殖腺における性分化関連遺伝子の発現 (ポスター発表)  [Not invited]
    萩原聖士, 山下量平, 山本真也, 後藤直英, 井尻成保, 足立伸次
    NGS現場の会第三回研究会  2013/09
  • Identification of genes involved in gonadal sex differentiation and the dimorphic expression pattern in undifferentiated gonads of Russian sturgeon Acipenser gueldenstaedtii  [Not invited]
    Hagihara Seishi, Yamashita R, Yamamoto S, Ijiri S, Adachi S
    7th International Symposium on Sturgeon  2013/07
  • 熱帯ウナギAnguilla celebesensisとA. marmorataの降海回遊生態  [Not invited]
    萩原聖士, 塚本勝巳
    平成25年度日本水産学会春季大会  2013/03
  • ニホンウナギ天然産卵親魚と人為催熟魚の卵膜の比較  [Not invited]
    泉ひかり, 萩原聖士, 黒木洋明, 張成年, 登坂亮太, 風藤行紀, 田中秀樹, 塚本勝巳, 香川浩彦, 亘理裕子, 工藤秀明, 井尻成保, 足立伸次
    平成25年度日本水産学会春季大会  2013/03
  • ロシアチョウザメの形態的未分化生殖腺における卵巣関連遺伝子の発現解析  [Not invited]
    後藤直英, 山下量平, 白瀧将人, 鈴木初美, 萩原聖士, 井尻成保, 足立伸次
    平成25年度日本水産学会春季大会  2013/03
  • チョウザメの性決定遺伝子・ウナギの卵質を規定する母性RNAの探索 —次世代シークエンサーを用いた挑戦への展望—  [Not invited]
    萩原聖士, 山下量平, 泉ひかり, 井尻成保, 足立伸次
    生命情報科学若手の会第4回研究会  2013/03
  • チョウザメの性決定遺伝子・ウナギの卵質を規定する母性RNAの探索 —次世代シークエンサーを用いた挑戦への展望— (ポスター発表)  [Not invited]
    萩原聖士, 山下量平, 泉ひかり, 井尻成保, 足立伸次
    生命情報科学若手の会第4回研究会  2013/03
  • Anguilla huangi Teng is a junior synonym of Anguilla luzonensis (poster presentation)  [Not invited]
    Watanabe S, Aoyama J, Hagihara Seishi, Ai B, Azanza R.V, Tsukamoto K
    The 15th annual meeting of East Asia Eel Resource Consortium (EASEC)  2012/11
  • 熱帯のジャングルで世界のウナギを獲る!! (市民講演)  [Not invited]
    萩原聖士
    東京国際科学フェスティバル2012 「うな展」  2012/09
  • スラウェシ島の熱帯ウナギAnguilla celebesensisとA. marmorataの降海回遊時の成熟状態と孕卵数  [Not invited]
    萩原聖士, 青山潤, 塚本勝巳
    平成24年度日本水産学会春季大会  2012/03
  • マリアナ沖のオオウナギ産卵親魚の生物学的特徴  [Not invited]
    萩原聖士, 青山潤, 脇谷量子郎, 望岡典隆, 泉ひかり, 瀧野陸也, 井尻成保, 足立伸次, 岡崎誠, 黒木洋明, 張成年, 塚本勝巳
    平成24年度日本水産学会春季大会  2012/03
  • フィリピン・ルソン島から新種記載されたウナギ属(Anguilla)魚類2種の分類に関する研究  [Not invited]
    渡邊俊, 青山潤, 萩原聖士, 阿井文瓶, Rhodora V. Azanza, 塚本勝巳
    平成24年度日本水産学会春季大会  2012/03
  • Downstream migration and spawning behavior of tropical eels in Sulawesi Island, Indonesia (poster presentation)  [Not invited]
    Hagihara Seishi
    The 3rd GCOE Joint Symposium between Kyushu Univ. and Univ. of Tokyo  2012/02
  • ウナギ属熱帯種Anguilla celebesensisとA. marmorataの銀化・降河回遊に伴う形態・生理変化  [Not invited]
    萩原聖士, 青山潤, 塚本勝巳
    平成23年度日本水産学会春季大会  2011/03
  • 人為催熟ニホンウナギと天然成熟ニホンウナギおよび天然成熟オオウナギの卵膜の厚さの比較 (ポスター発表)  [Not invited]
    泉ひかり, 瀧野陸也, 萩原聖士, 塚本勝巳, 黒木洋明, 張成年, 望岡典隆, 原佑輔, 脇谷量子郎, 登坂亮太, 風藤行紀, 田中秀樹, 亘理裕子, 井尻成保, 足立伸次
    平成23年度日本水産学会春季大会  2011/03
  • 北赤道海流域におけるオオウナギとニホンウナギの同所的産卵  [Not invited]
    黒木真理, 青山潤, Michael J. Miller, 吉永龍起, 篠田章, 萩原聖士, 塚本勝巳
    平成21年度日本水産学会春季大会  2009/03
  • 東北沖太平洋におけるイラコアナゴの分布と繁殖  [Not invited]
    萩原聖士, 伊藤正木, 服部努, 成松庸二, 佐藤美智男, 及川貴明, 塚本勝巳
    平成19年度東北ブロック底魚研究連絡会議  2008/03

Works

  • パラオの河川における熱帯ウナギ採集調査
    2012/06 -2012/06
  • 白鳳丸KH-12-02次航海. マリアナ諸島西方海域ウナギ産卵場生態調査
    2012/05 -2012/06
  • 白鳳丸KH-11-6次航海. マリアナ諸島西方海域ウナギ産卵場生態調査
    2011/06 -2011/07
  • 白鳳丸KH-11-4次航海. マリアナ諸島西方海域ウナギ産卵場生態調査
    2011/05 -2011/06
  • インドネシア・スラウェシ島の河川における熱帯ウナギの分布調査
    2010/10 -2010/11
  • 水産庁開洋丸航海. マリアナ諸島西方海域ウナギ産卵場生態調査
    2010/07 -2010/09
  • パラオの河川における熱帯ウナギ採集および魚類相調査
    2010/08 -2010/08
  • インドネシア・スラウェシ島中部スラウェシ州のトミニ湾流入河川における熱帯ウナギ採集調査
    2010/06 -2010/07
  • インドネシア・スラウェシ島のポソ川水系およびラ川水系における熱帯ウナギ採集調査
    2010/04 -2010/05
  • 白鳳丸KH-09-5次航海Leg.5,6,7,8. インド洋およびインドネシア周辺海域における熱帯ウナギの産卵場調査と回遊行動の追跡
    2010/01 -2010/03
  • インドネシア・バリ島の河川における熱帯ウナギ採集調査
    2010/02 -2010/02
  • インドネシア・スラウェシ島のポソ川水系における熱帯ウナギ採集調査
    2010/01 -2010/01
  • インドネシア・スラウェシ島のポソ湖およびその流入河川における熱帯ウナギ採集調査
    2009/11 -2009/12
  • 白鳳丸KH-09-1,2次航海. マリアナ諸島西方海域ウナギ産卵場生態調査
    2009/04 -2009/06
  • インドネシア・スラウェシ島のポソ湖における熱帯ウナギ採集調査
    2009/02 -2009/02
  • フィリピン・ルソン島カガヤン川水系におけるウナギ属未記載種採集調査
    2008/12 -2009/01
  • 淡青丸KT-08-31次航海. 天草近海における海洋産卵回遊ウナギの採集および電子データロガーの装着放流
    2008/11 -2008/12
  • 東北区水産研究所若鷹丸航海. 東北太平洋岸沖における底魚類資源量調査
    2008/10 -2008/11
  • 白鳳丸KH-08-1次航海. マリアナ諸島西方海域ウナギ産卵場生態調査
    2008/05 -2008/07
  • 兵庫県立香住高等学校但州丸航海 (東北区水産研究所用船). 東北太平洋岸沖におけるマダラ・スケトウダラ新規加入量調査
    2008/04 -2008/04
  • 東北区水産研究所若鷹丸航海. 東北太平洋岸沖における底魚類資源量調査
    2007/10 -2007/11
  • 白鳳丸KH-07-2次航海Leg.1,3. マリアナ諸島西方海域ウナギ産卵場生態調査
    2007/08 -2007/10
  • 東北区水産研究所若鷹丸航海. 東北太平洋岸沖および道東海域におけるカレイ類分布調査ならびに底魚類共同資源調査
    2007/06 -2007/07
  • 兵庫県立香住高等学校但州丸航海 (東北区水産研究所用船). 東北太平洋岸沖におけるマダラ・スケトウダラ新規加入量調査
    2007/04 -2007/04
  • 淡青丸KT-07-1次航海. 高知沖宮崎沖および種子島屋久島周辺海域における深海性ウナギ目魚類のビームトロール採集調査
    2007/02 -2007/02
  • 三重大学勢水丸航海. 熊野灘における深海性ウナギ目魚類の底延縄採集調査
    2006/11 -2006/11
  • 白鳳丸KH-06-2次航海Leg.2,3,5. マリアナ諸島西方海域ウナギ産卵場生態調査
    2006/06 -2006/09

MISC

  • 堀内萌未, 稲葉駿, 萩原聖士, 板倉光, 山下洋, 久米学, 塚本勝巳, 井尻成保, 足立伸次  日本水産学会大会講演要旨集(CD-ROM)  2021-  2021
  • 天然ニホンウナギの生殖腺の性分化 口頭
    堀内 萌未, 中司 大智, 長谷川 祐也, 萩原 聖士, 板倉 光, 山下 洋, 久米 学, 寺島 佑樹, 塚本 勝巳, 井尻 成保, 足立 伸次  東アジア鰻学会第2回研究発表会  2019/03/31  [Not refereed][Not invited]
  • 北海道産チョウザメ類の養殖産業化のための生産・加工体制整備
    足立伸次, 都木靖彰, 井尻成保, 萩原聖士, 三坂尚行, 古市明文, 市村政樹  ノーステック財団 研究開発助成事業2017 成果報告書  2017  [Not refereed][Not invited]
  • 萩原聖士, 須山喜市, 東典子, 堤尚信, 西尾朋高, 市村政樹, 三坂尚行, 鈴木渉太, 宮城大助, 古市明文, 市川卓, 松原創, 川崎琢真, 宗原弘幸, 高橋英祐, 山羽悦郎, 井尻成保, 足立伸次  日本水産学会大会講演要旨集  2016-  16  2016/03/26  [Not refereed][Not invited]
  • 北海道産チョウザメ類保護と養殖産業化のための種苗生産体制整備
    足立伸次, 井尻成保, 萩原聖士, 三坂尚行, 古市明文, 市村政樹  ノーステック財団 研究開発助成事業2016 成果報告書  27  -29  2016  [Not refereed][Not invited]
  • Transcriptional profiling of olfactory imprinting in the olfactory center of Pacific salmon
    MinowaY, Hagihara S, Abe T, Ijiri S, Adachi S, Kudo H  Chemical Senses  41-  (9)  e145  2016  [Not refereed][Not invited]
  • 北海道におけるチョウザメ養殖研究と産業化への取り組み
    萩原聖士  月刊養殖ビジネス  52-  (11)  47  -51  2015/10  [Not refereed][Invited]
  • 泉ひかり, 萩原聖士, 玄浩一郎, 井尻成保, 足立伸次  北海道大学水産科学研究彙報  65-  (3)  125  -130  2015  [Not refereed][Not invited]
     
    Removal of ribosomal RNAs(rRNA) from total RNA in eggs before preparation of a RNA library is required for the analysis of maternal mRNA using next-generarion sequencing ; this mRNA is essential for normal development. In mammals, the rRNA-depletion method is well-established and is already being used for the development of experiment kits. However, this method is not designed for other species, e.g., non-model organisms. In the current study, we attempted to remove rRNA from total RNA of Japanese eel Anguilla japonica, which is one of the most important aquaculture species in Japan. Removal was performed via hibridization-selection using mammalian probes from a commmercially available kit and/or an ordinally designed eel probe. Quantitative polymerase chain reaction(PCR) was performed to examine the depletion efficiency of 18S rRNA and 28S rRNA were comparative between each method, and >98% depletion was achieved. In addition, RNA-sequencing analysis using a next-generation sequencer and read-mapping analysis were carried out using the rRNA-depleted RNA, which was prepared using a mammalian probe, and mapping analysis revealed that rRNA reads were <1% of the total reads. These results suggest that an rRNA-depletion kit designed for a mammal can also be used for fish species.
  • 第7回国際チョウザメシンポジウム
    萩原聖士  日本水産学会誌 (水産研究のフロントから)  80-  (3)  2014/05  [Not refereed][Not invited]
  • 萩原聖士, 東典子, 井尻成保, 足立伸次  日本水産学会大会講演要旨集  2014-  100  2014/03/27  [Not refereed][Not invited]
  • Leptocephali collected during the KH-12-2 sampling survey
    Miller M.J, Hagihara Seishi, Watanabe S, Kuroki M, Aoyama J  Preliminary Report of the Hakuho Maru Cruise KH-12-2  43  -46  2012  [Not refereed][Not invited]
  • Anguilla japonica preleptocephali collected along the West Mariana Ridge, western North Pacific Ocean
    Miller M.J, Hagihara Seishi, Watanabe S, Inoue J.G, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-12-2  21  -26  2012  [Not refereed][Not invited]
  • Relationship between net-depth and wire-out of the 3 m ORI Big-Fish ring net
    Hagihara Seishi  Preliminary Report of the Hakuho Maru Cruise KH-11-4,6  45  2012  [Not refereed][Not invited]
  • XCTD and CTD observations during the KH-11-6 cruise
    Hagihara Seishi  Preliminary Report of the Hakuho Maru Cruise KH-11-4,6  11  -12  2012  [Not refereed][Not invited]
  • Anguilliform preleptocephali collected during the KH-11-4 and KH-11-6 cruise
    Miller M. J, Hagihara Seishi, Aoyama J, Mochioka N  Preliminary Report of the Hakuho Maru Cruise KH-11-4,6  32  2012  [Not refereed][Not invited]
  • Leptocephali collected during the KH-11-4 and KH-11-6 cruise
    Miller M.J, Hagihara Seishi, Aoyama J, Mochioka N  Preliminary Report of the Hakuho Maru Cruise KH-11-4,6  33  -34  2012  [Not refereed][Not invited]
  • First trial for tracking silver eel migration in Tomini Bay
    Aoyama J, Tsukamoto K, Hagihara Seishi, Triyanto, Tantu F.F, Manabe R, Sato K  Preliminary Report of the Hakuho Maru Cruise KH-09-5 Leg.7  17  -20  2012  [Not refereed][Not invited]
  • Test of a new type net for sampling of spawning Japanese eel
    Shinoda A, Murakami G, Yoshinaga T, Imai K, Watanabe S, Yokouchi K, Hagihara Seishi, Manabe R, Aoyama J, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-09-1,2  26  2012  [Not refereed][Not invited]
  • Functional morphology of the telescopic eye of leptocephali of the family Synaphobranchidae
    Hagihara Seishi, Miller M.J, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-08-1  37  2012  [Not refereed][Not invited]
  • Offshore transport of small marine eel larvae from the Ryukyu Ridge by a rare branch of the Kuroshio Current
    Miller M.J, Kitagawa T, Aoyama J, Yoshinaga T, Shinoda A, Hagihara Seishi, Okazawa H, Watanabe S, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-08-1  33  -36  2012  [Not refereed][Not invited]
  • Leptocephali collected during the KH-08-1 cruise
    Miller M.J, Otake T, Aoyama J, Hagihara Seishi, Okazawa H, Yoshinaga T, Shinoda A, Sudo R, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-08-1  26  -29  2012  [Not refereed][Not invited]
  • Genetic characterization of the assemblage of leptocephali around the Suruga seamount of the West Mariana Ridge
    Yoshinaga T, Miller M.J, Aoyama J, Shinoda A, Hagihara Seishi, Okazawa H, Watanabe S, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-08-1  30  -32  2012  [Not refereed][Not invited]
  • The biology of the deep-sea eels of the family Synaphobranchidae
    Hagihara Seishi, Miller M.J, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-07-2 Leg.1,3  30  2012  [Not refereed][Not invited]
  • Leptocephali collected during the KH-07-2 cruise
    Kuroki M, Miller M.J, Mochioka N, Okazawa H, Hagihara Seishi, Sawada E, Tawa A, Aoyama J, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-07-2 Leg.1,3  19  -20  2012  [Not refereed][Not invited]
  • Age, growth and haching date of Anguilla japonica
    Shinoda A, Kuroki M, Mochioka N, Sawada E, Hagihara Seishi, Yoshinaga T, Miller M.J, Aoyama J, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-07-2 Leg.1,3  25  2012  [Not refereed][Not invited]
  • Observations near a seamount using a Hooking Net
    Zenimoto K, Fukuda N, Shinoda A, Yokouchi K, Hagihara Seishi, Yamaoka N, Manabe R, Aoyama J, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-07-2 Leg.1,3  42  -43  2012  [Not refereed][Not invited]
  • HADEEP Lander Trials During the KH-06-2 Cruise
    Bailey D, Henriques C, Hagihara Seishi, Matsumoto A, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-06-2 Leg.2,3,5  36  -37  2012  [Not refereed][Not invited]
  • Leptocephali Collected During the KH-06-2 Cruise
    Miller M.J, Mochioka N, Otake T, Kuroki M, Hagihara Seishi, Sawada E, Aoyama J, Tsukamoto K  Preliminary Report of the Hakuho Maru Cruise KH-06-2 Leg.2,3,5  10  -11  2012  [Not refereed][Not invited]
  • 萩原聖士, 伊藤正木, 服部努, 成松庸二, 佐藤美智男, 及川貴明, 塚本勝巳  東北底魚研究  28-  48  -58  2008  [Not refereed][Not invited]

Awards & Honors

  • 2016/10 日本水産学会北海道支部 平成28年度日本水産学会北海道-東北合同支部大会 最優秀講演賞
     チョウザメ科種間雑種の発生/孵化および仔魚の生残における雑種強勢の可能性 
    受賞者: 萩原聖士
  • 2014/07 東アジア鰻資源協議会 公開シンポジウム うな丼の未来Ⅱ人とうなぎの共存をめざして 最優秀ポスター賞
     鰻の本場熱帯で熱帯ウナギの生態研究 
    受賞者: 萩原聖士

Research Grants & Projects

  • 水棲生物の繁殖イベントを検出する環境RNA・環境DNA分析法の開発と応用 ～サケマス類をモデルとして～
    河川財団：河川基金
    Date (from‐to) : 2023/04 -2025/03 
    Author : 萩原聖士
  • 生理生態学的アプローチによるウナギ属魚類の春機発動・銀化・回遊開始機構の解明
    日本学術振興会：科学研究費助成事業 基盤研究(C)
    Date (from‐to) : 2022/04 -2025/03 
    Author : 萩原聖士
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
    Date (from‐to) : 2021/04 -2025/03 
    Author : 木村 伸吾, 笠井 亮秀, 萩原 聖士, 脇谷 量子郎
     
    [輸送分散過程の数値シミュレーション解析] 主にEUのCopernicusが開発したデータ(GLOBAL OCEAN PHYSICS REANALYSIS)を使用して、オオウナギ北太平洋集団の産卵場（西マリアナ海嶺南部海域）からの仔魚輸送分散シミュレーションを実施した。その結果、北赤道海流によって西方に輸送された後、黒潮に取り込まれて北上するものとミンダナオ海流に取り込まれて南下する輸送経路を再現し、両者の割合には季節的・経年的変動があることを明らかにした。 [ニホンウナギとオオウナギを対象とした成魚の行動調査および人工構造物の影響評価] 養殖ニホンウナギの放流後の行動を調べるために、養殖ウナギ170個体を静岡県の深田川に放流した。携帯型RFIDシステムを用いて経時的追跡調査を実施したところ、上流移動が落差によって制限されることや、肥満度が低いほど下流移動確率が高まることが示された。また、静岡県の道龍川で採集したニホンウナギ49個体と奄美大島の大棚川で採集したオオウナギ106個体の水系内移送放流を行い、経時的追跡調査を実施した。その結果、10日後の元の生息地への回帰率はニホンウナギの場合には上流移送群が29.2%、下流移送群が16.0%、オオウナギの場合には上流移送群が3.7%、下流移送群が36.5%であり、種特異的な生息地固執性・選好性の存在が示唆された。 [環境DNA分析のための採水・濾過および解析] 日本産ウナギ属魚類4種を対象に、環境DNA検出/定量用の種特異的プライマー・プローブセットを設計した。房総半島、屋久島、奄美大島、石垣島、西表島の20河川、計51地点において採水を行い、試料を収集した。また、南西諸島海域で行った学術研究船「白鳳丸」による調査データの遺伝子解析を継続して実施し、その際に採集した仔稚魚の種同定結果との比較を行う準備を進めた。
  • 分野横断型研究による日本列島におけるニホンウナギ分布変遷の復元
    中央大学：2022年度中央大学共同研究プロジェクト
    Date (from‐to) : 2022/04 -2024/03
  • 熱帯性魚類と温帯性魚類の春機発動における環境刺激の役割
    東京大学：研究者サポート要員配置助成
    Date (from‐to) : 2021/04 -2022/03 
    Author : 萩原聖士
  • 降海か、残留か：ニホンウナギの春機発動が回遊開始メカニズムに果たす役割
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
    Date (from‐to) : 2019/04 -2022/03 
    Author : 萩原 聖士
     
    川や湖で一定の体サイズ以上に成長したウナギは、秋に銀化して産卵回遊を開始する。回遊を開始する体サイズと年齢は個体変異に富み、どんな生態学的条件を満たした個体が、どのような生理学的プロセスを経て、春機発動・銀化・回遊開始に至るかは明らかでない。これを解明すれば、ウナギの生殖生理と回遊現象の理解を大きく深めることができる。 本研究課題では、夏季に天然水系で採集した全長50cm以上のニホンウナギの黄ウナギ39個体（6月20個体、8月19個体）から、バイオプシー (生検) による卵巣試料採取および採血を行い、PITタグによる個体識別を行った後に自然環境を模した大型野外実験池に放流した。その後、11月に放流個体を回収して銀化状態を判別し、秋に銀化する個体と銀化しない個体が、夏季の時点でどのような生殖生理学的特徴を持っているのか調べた。 11月に実験池から放流個体の回収を試みたところ、34個体が回収され（回収率87%）、そのうち7個体が体側や胸鰭の金属光沢など銀化の兆候を示した。これらの7個体は眼径指数や胸鰭長指数が高く、銀ウナギに典型的な形態的特徴を有していた。 夏季の放流前に採取した卵巣試料を用いて最大卵群卵径の測定を行ったところ、秋に銀化した個体は銀化しなかった個体に比べて、最大卵群卵径が有意に大きかった。また、組織観察を行ったところ、秋に銀化した個体は銀化しなかった個体に比べて卵母細胞の発達が進行していた。さらに、血中の性ステロイドを比較したところ、秋に銀化した個体は銀化しなかった個体に比べて夏季の11-KTの血中濃度が有意に高かった。 以上より、秋に銀化して回遊開始するウナギは、少なくとも初夏の時点で春機発動が生じていると考えられた。この結果は、銀化などの回遊関連の形態的・生理的変化は水温が低下する秋に生じるという従来の常識を覆すものである。
  • ウナギ属魚類の春機発動と銀化に関する生理生態学的研究
    東京大学：大気海洋研究所ワーク・ライフ・バランス支援
    Date (from‐to) : 2021/04 -2021/09 
    Author : 萩原聖士
  • 天然の生態情報に基づくウナギ親魚の新しい催熟法の開発
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
    Date (from‐to) : 2019/06 -2020/03
  • 北海道産チョウザメ類の養殖産業化のための生産・加工体制整備
    ノーステック財団：発展・橋渡し研究補助金
    Date (from‐to) : 2016/08 -2017/03 
    Author : 足立伸次
  • 北海道産チョウザメ類保護と養殖産業化のための種苗生産体制整備
    ノーステック財団：スタートアップ研究補助金
    Date (from‐to) : 2015/08 -2016/03 
    Author : 足立伸次
  • ノーステック財団：若手研究人材・ネットワーク育成補助金
    Date (from‐to) : 2014/07 -2015/03 
    Author : 萩原聖士

Educational Activities

Teaching Experience

  • Aquatic Science Special Lecture IAquatic Science Special Lecture I Hokkaido University

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