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Last Updated :2024/10/02

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Master

Affiliation (Master)

  • Faculty of Fisheries Sciences　Marine Bioresource and Environmental Science　Marine Bioresource Science

Affiliation (Master)

  • Faculty of Fisheries Sciences　Marine Bioresource and Environmental Science　Marine Bioresource Science

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Profile and Settings

Profile and Settings

  • Name (Japanese)

    Nakaya

  • Name (Kana)

    Mitsuhiro

  • Name

    201201054204422091

Achievement

Research Interests

  • feeding habits   growth and survival   Early life history   Fisheries bio-resources   

Research Areas

  • Life sciences / Aquaculture / Fisheries bio-resources

Research Experience

  • 2018/05 - Today Hokkaido University Faculty of Fisheries Sciences
  • 2012/09 - 2018/04 Hokkaido University School of Fisheries Sciences
  • 2010/11 - 2012/08 Fisheries Research Agency
  • 2009/03 - 2010/10 カキキン有限会社 生産・販売・研究職員
  • 2006/10 - 2009/01 Japan International Cooperation Agency
  • 2006/04 - 2006/09 カキキン有限会社 生産・販売・研究職員
  • 2004/04 - 2006/03 Fisheries Research Agency

Education

  • 2001/04 - 2004/03  北海道大学大学院
  • 1999/04 - 2001/03  北海道大学大学院
  • 1995/04 - 1999/03  Hokkaido University  School of Fisheries Sciences

Awards

  • 2014/03 The Japanese Society of Fisheries and Technology The Japanese Society of Fisheries and Technology Encouragement Prize in 2013
     The acquisition of the biological information to contribute the population dynamics model of Pacific saury by captive experiment 
    受賞者: Mitsuhiro Nakaya
  • 2011/03 The Japanese Society of Fisheries Science Research article award of the Japanese Society of Fisheries Science in 2011
     Growth and maturation of Pacific saury Cololabis saira under laboratory conditions. 
    受賞者: M. Nakaya;T. Morioka;K. Fukunaga;N. Murakami;T. Ichikawa;S. Sekiya;S. Suyama

Published Papers

  • Tomoya Ishikawa, Mitsuhiro Nakaya, Yuta Eno, Tetsuya Takatsu
    J Fish Biol 2024/07 [Refereed][Not invited]
  • Tetsuya Takatsu, Tomoaki Toyonaga, Shin’ya Hirao, Eri Ooka, Naoto Kobayashi, Mitsuhiro Nakaya
    Fisheries Science 90 (3) 435 - 452 0919-9268 2024/04/03
  • Ryusei Ohtani, Jun Yamamoto, Takashi Yanagimoto, Kohsuke Adachi, John R Bower, Hidefumi A Fujioka, Miyuki Hirose, Mitsuhiro Nakaya, Satoshi Suzuki, Shun Tokioka
    Journal of Molluscan Studies 88 (3) 0260-1230 2022/09/19 [Refereed][Not invited]
     
    ABSTRACT The genetic diversity of sperm and spermatangia stored by mated Japanese flying squid (Todarodes pacificus) was examined to better understand how this ommastrephid squid mates and stores sperm. Analyses were conducted using spermatangia implanted in the lips of immature squid collected in Tsugaru Strait, northern Japan, in July–August 2019, spermatangia implanted in the lips of mature squid collected in the East China Sea in April 2019, and sperm stored in the seminal receptacles of squid collected off Tosashimizu, Kochi Prefecture, Japan, in February 2019. DNA was extracted, microsatellite loci were amplified using five PCR primers, and allele peaks were analysed. Analysis of the spermatangia confirmed that females in the field mate with multiple males, suggesting that males transfer from 1 to at least 14 spermatangia in each mating. Analysis of the seminal receptacles showed that sperm from multiple males are mixed within individual sperm chambers. Spermatangia were also found implanted in the lips of males, providing evidence of same-sex sexual behaviour. To date, studies of cephalopod mating systems have focused on coastal species, and the results of this study add to our growing knowledge of more oceanic species.
  • Shota Hosono, Takahiro Irie, Jun Yamamoto, Mitsuhiro Nakaya, Yasunori Sakurai, Tomohiko Kawamura, Yoko Iwata
    Journal of the Marine Biological Association of the United Kingdom 1 - 7 0025-3154 2022/08/19 [Refereed]
     
    Abstract The strontium-to-calcium ratio (Sr/Ca) in aragonitic shells and statoliths often depends on temperature, and has been investigated for its potential availability as a sclerochronological record of the temperatures experienced during the life of the animal. Only a few cephalopod species have been subjected to rearing experiments to validate the temperature dependence of statolith Sr/Ca despite the strong demand for estimating their historical ecology, particularly of migratory squid populations. In this context, we examined the relationship between temperature and statolith Sr/Ca in the spear squid, Heterololigo bleekeri, by maintaining wild-caught immature individuals at one of three constant temperatures: 12, 14 or 16°C. A portion of statolith precipitated during the experiment was optically identified by daily increment analysis, and then subjected to Sr/Ca measurement using an electron probe micro-analyser. Regression analysis (N = 29) demonstrated the dependence of statolith Sr/Ca on temperature and the relationship was estimated as an equation Y = 9.93 (±0.29) − 0.11 (±0.02) X. However, ~90% of the total residual variance was accounted for by the among-individual variation of statolith Sr/Ca within each temperature group. Consequently, the 95% confidence interval ranged over ± 7.85°C when the temperature was estimated by inserting a Sr/Ca value into this equation. The statolith Sr/Ca values are unlikely to provide reliable estimates for absolute temperatures, but it may allow reconstruction of a time-series of relative temperatures experienced by a particular individual.
  • Keitaro Kajiwara, Mitsuhiro Nakaya, Kota Suzuki, Yota Kano, Tetsuya Takatsu
    Fisheries Oceanography 31 (3) 238 - 254 1054-6006 2022/05
  • Tomoya Ishikawa, Mitsuhiro Nakaya, Weifeng Gao, Tetsuya Takatsu, Kenji Odani, Ryo Suzuki, Kyosei Noro, Yuhei Takeya
    Environmental Biology of Fishes 105 (2) 303 - 312 0378-1909 2022/02
  • Tomoya Ishikawa, Mitsuhiro Nakaya, Tetsuya Takatsu
    Environmental Biology of Fishes 105 (1) 77 - 86 0378-1909 2022/01
  • Effects of water temperature on the embryonic period of Pacific rainbow smelt Osmerus dentex.
    T. Nam, M. Nakaya, T. Ishikawa, T. Takatsu
    Aquaculture Science 69 (3) 223 - 225 2021/10 [Refereed][Not invited]
  • Age and growth of Pacific rainbow smelt Osmerus dentex estimated from scales and otoliths in Funka Bay and the Yamazaki River, Japan.
    T. Nam, M. Nakaya, T. Takatsu
    Aquaculture Science 69 (2) 153 - 161 2021/07 [Refereed][Not invited]
  • W. Gao, M. Nakaya, T. Ishikawa, T. Takatsu, Y. Takeya, R. Suzuki, K. Noro
    Fisheries Science 87 (4) 541 - 548 0919-9268 2021/06 [Refereed][Not invited]
  • Note on color variations of inner surface of pectoral fins in Lepidotrigla microptera Günther, 1873 (Actinopterygii: Triglidae) from Mutsu Bay, Japan.
    N. Kawakami, T. Kawai, M. Nakaya
    The Thailand National History Museum Journal 15 (1) 51 - 58 2021/06 [Refereed][Not invited]
  • Feeding habits of larval yellow goosefish Lophius litulon around the Shimokita Peninsula and in Funka Bay, Japan
    Weifeng Gao, Mitsuhiro Nakaya, Tetsuya Takatsu, Yuhei Takeya, Kyosei Noro
    Aquaculture Science 68 (3) 275 - 277 2020/09 [Refereed][Not invited]
  • 高 偉峰, 中屋光裕, 髙津哲也, 竹谷裕平, 鈴木 亮, 野呂恭成
    水産海洋研究 84 (3) 228 - 229 0916-1562 2020/08 [Not refereed][Invited]
  • ホッケの父母性効果について
    森田晶子, 横田高士, 中屋光裕, 横山貴洋
    水産海洋研究 84 (3) 225 - 225 2020/08 [Not refereed][Invited]
  • ホッケ生活史初期の成長
    河村眞美, 中屋光裕, 髙津哲也, 巣山哲, 鈴木祐太郎, 高畠信一
    水産海洋研究 84 (3) 223 - 224 2020/08 [Not refereed][Invited]
  • Close-up observations on the spawning behavior of a captive Japanese flying squid (Todarodes pacificus)
    J. Yamamoto, K. Adachi, J. Bower, H. Matsui, M. Nakaya, R. Ohtani, P. Puneeta, S. Suzuki, S. Tokioka, D. Vijai, T. Yanagimoto, H. Yoo
    Scientific Reports 2019 [Refereed][Not invited]
  • P. Hong, S. Katayama, M. Yamamoto, M. Ishii, T. Baba, M. Saeki, M. Suzuki, M. Nakaya
    Asian Fisheries Science 32 (2) 72 - 80 0116-6514 2019 [Refereed][Not invited]
     
    © Asian Fisheries Society. The marbled flounder, Pseudopleuronectes yokohamae (Günther, 1877), is one of the most economically important flatfish species in Japan. Research has been carried out on the age and growth of this species. However, there has been no comparison of growth rates of the fish from different locations. In this study, the age composition and growth trajectory of marbled flounder from different coastal waters in Japan were estimated and compared. Samples were collected from six locations, namely in the prefectures of Kagawa, Yamaguchi, Chiba, Niigata, Miyagi and Hokkaido. Age composition and growth trajectories were determined by analysing otoliths. The majority of specimens were found to be 2 to 3 years of age. Female marbled flounder were found to have a higher total length (TL) and growth rate than males. There were significant differences in growth curves for each of the sampling locations. There were also significant differences between males and females at each sampling location, with females attaining a higher theoretical maximum TL, longer lifespan and faster growth rate than males. The data from the current study can be used to help improve fish resource management.
  • Annual and seasonal changes in the assemblage of planktonic copepods and appendicularians in Funka Bay before and after intrusion of Coastal Oyashio Water.
    H. Yamaoka, T. Takatsu, K. Suzuki, N. Kobayashi, A. Ooki, M. Nakaya
    Fisheries Science 85 (6) 1077 - 1087 2019 [Refereed][Not invited]
  • Growth of the brittle star Ophiura sarsii sarsii in Funka Bay, Hokkaido, Japan.
    K. Orino, K. Ishigane, K. Suzuki, J. Izumiura, M. Nakaya, T. Takatsu
    Fisheries Science 85 (4) 705 - 716 2019 [Refereed][Not invited]
  • CHOW SEINEN, NIGORIKAWA NAOKO, URAGAKI NAOKO, KAWAMURA AKIKO, ICHIKAWA TATSUYA, USHIODA KENTARO, HIGUCHI MASAHITO, TEGA TARO, KODAMA KOJI, ITOH MASAHIRO, ICHIMURA MASAKI, IMAI TADASHI, MATSUZAKI KOJI, HIRASAWA KEI, TOKURA KEITA, NAKAHATA KATSUMI, KODAMA SAKIE, HAKOYAMA HIROSHI, YADA TAKASHI, NIWA KENTARO, NAGAI SATOSHI, YANAGIMOTO TAKASHI, IKEDA MINORU, SAITO KAZUYOSHI, NAKAYA MITSUHIRO, MARUYAMA TOMOAKI, MAKI SOICHIRO, OONUKI TAKAKIYO, MUTO FUMITO, NOHARA KENJI, FURUSAWA CHIHARU, SHICHIRI HIROSHI
    NIPPON SUISAN GAKKAISHI 公益社団法人 日本水産学会 84 (4) 674 - 681 0021-5392 2019 [Refereed][Not invited]
     

    Among the palaemonid shrimp species of the genus Palaemon known from Japan, Palaemon paucidens is the only species inhabiting freshwater areas. Two genetically distinct types (designated by A and B) were found by allozyme electrophoresis analysis three decades ago. We developed a simple multiplex-PCR assay to discriminate the two types based on 18S rDNA sequences. A total of 422 individuals collected at 152 localities in Japan were analyzed by the multiplex-PCR assay. No heterozygote was observed, indicating that these two types have been reproductively isolated. Both types were distributed throughout Japan; type A was observed in ponds, lakes and rivers, while type B was observed only in rivers.

  • ミトコンドリアDNAによって検討した噴火湾に生息するアカガレイ個体群の遺伝的多様性．
    山本祐樹, 池田実, 中屋光裕, 鈴木孝太, 髙津哲也
    魚類学会誌 65 (1) 75 - 80 2018/04 [Refereed][Not invited]
  • Yuhei Takeya, Tetsuya Takatsu, Tomoyuki Yamanaka, Yasutoki Shibata, Mitsuhiro Nakaya
    Nippon Suisan Gakkaishi (Japanese Edition) 84 (1) 130 - 132 1349-998X 2018 [Refereed][Not invited]
  • Savitri Marannu, Mitsuhiro Nakaya, Tetsuya Takatsu, Shin-ichi Takabatake, Mikimasa Joh, Yutaro Suzuki
    FISHERIES RESEARCH 194 129 - 134 0165-7836 2017/10 [Refereed][Not invited]
     
    We investigated the relationship between morphological development in the embryonic stage and the formation of otolith microstructure in Arabesque greenling Pleurogranunus azonus, and then validated that the otolith increments were formed on a daily basis. Increments were more consistent to count and measure in sagittal otoliths from wild caught larvae compared to lapillar otoliths, and we suggest that sagittal otoliths as suitable for otolith microstructure analysis. This study clarified the formation time of three prominent increments on the sagittal otolith. First prominent increment (approx. 17 mm in otolith radius (OR)) was coincident with the time of eye pigmentation in the embryonic stage. Second (approx. 36 pm OR) was considered to be the hatch increment and the third (approx. 38 pm OR) was at the time of transition from endogenous to exogenous nutrition. The third prominent increment which was the clearest radius (termed below as the check) was used as the starting point for validating the daily increment formation in sagittal otolith, and also there was no significant difference in the check radius among reared (6, 8, and 10 degrees C) and wild caught larvae. The relationship between number of days after hatching and the number of increments formed after check was significant and the slope of the regression line was not different from 1, validating the assumption that growth increments are formed on a daily basis in sagittae of P. azonus. The check was formed on the otolith at the transition from endogenous to exogenous nutrition, and the number of days required prior to its formation varied with water temperature. For the species in which the check is formed at the nutritional transition from endogenous to exogenous feeding, the relationship between number of days to check formation and water temperature is essential to estimate the hatch date of wild caught individuals.
  • M. Nakaya, Y. Takeya, K. Noro, R. Suzuki, W. Gao, T. Takatsu
    Aquaculture Science 日本水産増殖学会 65 (3) 251 - 254 0371-4217 2017/09 [Refereed][Not invited]
  • M. Nakaya, S. Marannu, Y. Inagaki, K. Kajiwara, Y. Sato, K. Suzuki, T. Takatsu
    Aquaculture Science 日本水産増殖学会 65 (3) 247 - 250 0371-4217 2017/09 [Refereed][Not invited]
  • Kota Suzuki, Mitsuhiro Nakaya, Hae-Kyun Yoo, Taihei Matsuda, Tetsuya Takatsu
    NIPPON SUISAN GAKKAISHI 83 (4) 580 - 588 0021-5392 2017/07 [Refereed][Not invited]
     
    To assess transportation in the early life history stages in the flathead flounder Hippoglosoides dubius, we measured the specific gravities of the eggs at 9 degrees C and the larvae and juveniles at 9 degrees C and 12 degrees C using density gradient and density-bottle methods. We also examined the effect of temperature (1, 3, 6, 9, and 12 degrees C) on the duration between fertilization and yolk sac absorption. Eggs in glass beakers had lower specific gravities than the water densities experienced in one of the spawning grounds, Funka Bay. In contrast, the specific gravities were higher than the water densities in Funka Bay in yolk sac larvae, almost the same in early preflexion larvae, and increased with advancing development from the late preflexion larval to juvenile stages. The duration from fertilization to yolk sac period decreased with increasing water temperature. Thus, annual variation in the water temperature in the field combined with transport directions and speeds at various depths may affect the transportation of flounder eggs and larvae.
  • Yuhei Takeya, Tetsuya Takatsu, Tomoyuki Yamanaka, Yasutoki Shibata, Mitsuhiro Nakaya
    NIPPON SUISAN GAKKAISHI 83 (1) 9 - 17 0021-5392 2017 [Refereed][Not invited]
     
    Yellow goose fish Lophius litulon is an economically valuable fish species around Japan. The aim of the present study was to evaluate a suitable technique for age determination of yellow goose fish using the illicium. Specimens of yellow goose fish were collected by commercial boats and research vessels from the Sea of Japan, Tsugaru Strait and the Pacific Ocean off Aomori Prefecture from November 2013 to March 2016 using bottom gill, set and trawl nets. Each specimen was measured and sexed, and the illicium and 8th vertebra were removed in the laboratory. The epidermis of each illicium was removed after boiling, and the illicia were dried, whereas each of the 8th vertebra was boiled and sectioned. We found that the best ageing method was a count of opaque zones in the illicia. This was facilitated using the cross-sectioned illicia that had been etched with 1 mol/L HCl for 30 s, stained with methylene blue for 4 h and viewed under transmitted and incident light. Opaque zones form twice per year ( mainly during June and November. December) in the illicia. Age determination using opaque zones in the illicia was more accurate than that using opaque zones in the vertebral centra. Because the illicia of yellow goose fish are easy to collect and manipulate for age determination and the growth rate estimated using the illicia and tagging experiments is similar, this method of age determination may be useful for these fish in this region.
  • Satoshi Suyama, Akio Shimizu, Sayoko Isu, Hitomi Ozawa, Taizo Morioka, Mitsuhiro Nakaya, Toru Nakagawa, Naoto Murakami, Takashi Ichikawa, Yasuhiro Ueno
    FISHERIES SCIENCE 82 (3) 445 - 457 0919-9268 2016/05 [Refereed][Not invited]
     
    Rearing experiments under controlled temperature conditions were carried out to establish a methodology for estimating the spawning history of the Pacific saury Cololabis saira. The experiments involved histological examination of ovarian arterioles at various maturational stages. We found that pre-spawning immature fish lacked Victoria blue (VB)-positive arterioles in the ovaries, as did maturing fish, while post-spawning females had VB-positive arterioles. The latter were also observed after 6 months when most spawning activity had ceased. The results indicate that VB-positive ovarian arterioles are a characteristic feature of actively spawning Pacific saury and that females with VB-positive arterioles can be classified as post-spawning fish. Females without VB-positive arterioles can be regarded as "practically unspawned" fish, and this group may include some fish that have spawned a number of times, in addition to completely unspawned fish. The methodology has many practical uses such as in the estimation of maturation rates for stock management.
  • Tadao Jinbo, Shigeki Dan, Mitsuhiro Nakaya, Masakazu Ashidate, Katsuyuki Hamasaki
    FISHERIES SCIENCE 79 (3) 459 - 467 0919-9268 2013/05 [Refereed][Not invited]
     
    The effect of varying levels of dietary n-3 highly unsaturated fatty acid (HUFA) content in Artemia sp. on survival, the number of days required to reach each larval stage, and the growth and morphogenesis of larvae of the horsehair crab Erimacrus isenbeckii were examined. Artemia enriched with materials of six types [condensed Nannochloropsis sp., ethyl oleate, two enrichment levels of eicosapentaenoic acid (EPA), and those of docosahexaenoic acid], and unenriched Artemia were fed to larvae. Newly hatched larvae were reared in groups of 50 individuals in two 2-l beakers until they reached the first crab stage. No significant difference was found in survival rate, growth, or morphogenesis of larvae, but fewer days tended to be required to reach the first crab stage in the group enriched with high EPA compared to the other groups. Results show that, although the amount of n-3 HUFA in unenriched Artemia might meet the demands of horsehair crab larvae for their survival, growth, and morphogenesis, Artemia should be enriched with high EPA to improve the rate of larval development.
  • Mikimasa Joh, Mitsuhiro Nakaya, Naoto Yoshida, Tetsuya Takatsu
    MARINE ECOLOGY PROGRESS SERIES 494 267 - 279 0171-8630 2013 [Refereed][Not invited]
     
    Flatfishes drastically change their habitat, body form, and feeding during metamorphosis; thus, the early juvenile and larval stages are viewed as being critical for early survival. However, to the best of our knowledge, no studies have tested the growth-mortality hypothesis for both larval and juvenile stages of flatfishes. Therefore, we investigated the relationship between growth rate and environmental factors and tested the hypothesis for both larval and juvenile stages of marbled sole Pseudopleuronectes yokohamae in Hakodate Bay, Japan from 2001 to 2003. For both larval and juvenile stages, otolith growth correlated with water temperature. Eye-migrating larvae were defined as survivors of planktonic life (SVpelagic). Large juveniles captured in late June and July were defined as survivors of shrimp predation (SVjuvenile). To test the growth-mortality hypothesis, otolith growth was compared between the SV and the original population. During the pelagic larval stage, growth-selective survival was not detected in any of the 3 yr. During the early juvenile stage, fast-growing individuals survived selectively in 2002 but not in 2003. In 2002, population growth of juveniles was slow because water temperatures were low. Thus, juveniles in 2002 required time to exceed the size spectrum that is vulnerable to shrimp predation; consequently, the individuals that grew more rapidly were able to survive selectively. Our results show the importance of the early juvenile stage for the survival of flatfishes.
  • Mitsuhiro Nakaya, Kyohei Fukunaga, Shigenori Suzuki
    Acta Ichthyologica et Piscatoria 43 (1) 75 - 77 0137-1592 2013 [Refereed][Not invited]
     
    The body proportions of 0-group Pacific herring, Clupea pallasii, were compared between hatcheryreared-and wild fish and significant differences were observed. There were fewer vertebrae (Mean ± SD) in hatchery-reared-(52.5 ± 2.15) than in wild fish (53.7 ± 0.92). For both the hatchery-reared-and the wild individuals, the correlations between number of vertebrae and vertebral column length (VCL) per standard length (SL) were positive. Head length (HL) was approximated as SL minus VCL. Therefore, the proportion of HL in relation to SL became lower with the higher number of vertebrae. Their vertebral number may relate to the difference of body proportions between hatchery-reared-and wild fish.
  • Mitsuhiro Nakaya, Takuzo Abe
    ICHTHYOLOGICAL RESEARCH 58 (4) 377 - 381 1341-8998 2011/11 [Refereed][Not invited]
     
    A total of 45 juvenile [30.0-57.4 mm total length (TL)] slime flounder Microstomus achne were collected in the coastal area of southern Hokkaido from April to July in 2001 and April to June in 2002. Their diets were analyzed. Slime flounder juveniles of 30.0-39.9 mm TL fed predominantly on small crustaceans (gammarid amphipods, harpacticoids and cumaceans) and those of 40.0-57.4 mm TL on gammarid amphipods, cumaceans and polychaetes. The major prey items changed with growth from small crustaceans (e.g., harpacticoids) to polychaetes, although gammarid amphipods were the major prey items throughout the juvenile period (30.0-57.4 mm TL).
  • Mitsuhiro Nakaya, Taizo Morioka, Kyohei Fukunaga, Naoto Murakami, Takashi Ichikawa, Sachio Sekiya, Satoshi Suyama, Yasuhiro Ueno, Akio Shimizu
    ENVIRONMENTAL BIOLOGY OF FISHES 92 (1) 113 - 123 0378-1909 2011/09 [Refereed][Not invited]
     
    To understand the survival processes in Pacific saury during the early life stages, growth histories were compared between individuals that died and those that survived under laboratory conditions. The morphology of Pacific saury drastically changed by 40 day after hatching (DAH) under 20A degrees C, and during this period most individuals reached the juvenile stage (40 mm knob length (KnL)). From hatch to this period, high levels of mortality were observed. The back-calculated growth rates analyzed from otolith microstructure were compared among individuals that died and those that survived this period of metamorphosis. Growth rates of individuals that died were significantly lower since 1 DAH than surviving individuals. Factors contributing to mortality are considered to be the cumulative effects of their delayed growth and their less favorable body conditions (growth dependent survival). The body size (40 mm KnL) that is currently used to estimate the recruitment is concluded to be valid for evaluating the recruitment in the field.
  • 平成22年度日本水産学会論文賞受賞 Growth and maturation of Pacific saury Cololabis saira under laboratory conditions.
    中屋 光裕
    日本水産学会誌 77 571  2011 [Not refereed][Invited]
  • Mitsuhiro Nakaya, Taizo Morioka, Kyouhei Fukunaga, Naoto Murakami, Takashi Ichikawa, Sachio Sekiya, Satoshi Suyama
    FISHERIES SCIENCE 76 (1) 45 - 53 0919-9268 2010/01 [Refereed][Not invited]
     
    This study details the growth and maturation processes of Pacific saury Cololabis saira from eggs to first spawning under laboratory conditions. They were reared at 20A degrees C, and fed almost to satiation every day. There was no significant difference in the knob length (KnL) between males and females, and so data for both sexes were combined in the following Gompertz growth formula: KnL (t) = 277.1 exp[-exp(-0.015 (t - 83.8))]. The first spawning was observed 243 days after hatching (DAH). At the beginning of spawning, when the mean KnL was about 250 mm, only several hundred eggs were spawned at most. The spawning continued, and the number of spawned eggs increased notably after 260 DAH. Correlation and stepwise multiple regression analysis of the gonad somatic index (GSI) of Pacific saury versus KnL, CF, and DAH revealed that only DAH (R = 0.88 and 0.72 for males and females, respectively) was significantly correlated with GSI of Pacific saury (P < 0.001). This result suggests that DAH is one of the most influential factors for maturation in this species.
  • M Nakaya, T Morioka, K Fukunaga, N Murakami, T Ichikawa, S Sekiya
    Aquaculture Science 水産増殖談話会 58 (2) 301 - 303 0371-4217 2010 [Refereed][Not invited]
     
    魚類、サンマ科、飼育、死亡率、資材、利用。
  • NAKAYA Mitsuhiro, MORIOKA Taizo, FUKUNAGA Kyohei, MURAKAMI Naoto, ICHIKAWA Takashi, SEKIYA Sachio, SUYAMA Satoshi
    Aquaculture Science 水産増殖談話会 57 (4) 625 - 626 0371-4217 2009/12/20 [Not refereed][Not invited]
     
    魚類、サンマ化、稚仔、給餌、生存、水温。
  • Mikimasa Joh, Tetsuya Takatsu, Mitsuhiro Nakaya, Naoto Yoshida, Masayasu Nakagami
    FISHERIES SCIENCE 75 (3) 619 - 628 0919-9268 2009/05 [Refereed][Not invited]
     
    To examine survival processes in marbled sole Pseudopleuronectes yokohamae larvae, nutritional transition date distributions (NTDDs) were compared between larvae and large juveniles (LJs) in Hakodate Bay from 2001 to 2003. NTDDs were used instead of hatch date distributions because the onset of increment formation coincides with the timing of the transition from endogenous to exogenous nutritional sources for marbled sole. LJs were defined as being large enough to be safe from predation by the sand shrimp Crangon uritai. In 2001 and 2002, the ranges of the NTDDs of LJs largely overlapped with those of the larvae. However, in 2003, the early cohort (those whose otolith rings formed before 17 March) disappeared from the NTDDs during and after April, and the NTDDs of LJs were heavily biased toward a later period compared to those of the larvae. In 2003, Oyashio coastal water (< 3A degrees C and a parts per thousand currency sign33.0 salinity) unexpectedly intruded into the bay in late March and lowered the water temperature from 6.3A degrees C on 6 March to 4.1A degrees C on 25 March. One possible cause of mortality in the early cohort of 2003 may be feeding failure caused by the extremely low water temperature.
  • Mitsuhiro Nakaya, Taizo Morioka, Yohei Fukunaga, Sachio Sekiya, Tadao Jinbo, Hiroshi Nagashima, Yasuhiro Ueno
    FISHERIES RESEARCH 93 (1-2) 186 - 189 0165-7836 2008/09 [Refereed][Not invited]
     
    Daily ring formation was verified by examining the growth of the marginal increment on sagittal otoliths of larval and juvenile Japanese halfbeak Hyporhamphus sajori. The relationship between age (x) and number of increments (y) is y = 1.0x + 2.0. The first daily increment was formed during the 2 days before hatching. The relationship between the number of rings deposited after the alizarin complexone (ALC) mark (x) and the number of increments (y) is given by: y = 1.0x-0.2. The index of completion of the Marginal increment was 99 +/- 4.1% (mean +/- S.D.) at 04:00, and from 24 +/- 4.4% at 08:00 it increased with time of clay and reached 98 +/- 3.6% until the next day at 04:00. Growth of the incremental zone started a few hours after sunrise prior to which the discontinuous zone seemed to be formed. Light rhythms tend to be one of the most important factors for the formation of the marginal increment on otoliths. Based oil the relationships between time of day and the marginal increment on otoliths, it Would be possible to estimate the predation time for specimens retrieved from stomach contents, and also clearer analysis of the growth history immediately prior to the sampling time. (c) 2008 Elsevier B.V. All rights reserved.
  • Tomomi Higashitani, Tetsuya Takatsu, Mitsuhiro Nakaya, Mikimasa Joh, Toyomi Takahashi
    JOURNAL OF SEA RESEARCH 58 (1) 78 - 89 1385-1101 2007/07 [Refereed][Not invited]
     
    Maternal effects of animals are the phenotypic influences of age, size, and condition of spawners on the survival and phenotypic traits of offspring. To clarify the maternal effects for marbled sole Pseudopleuronectes yokohamae, we investigated the effects of body size, nutrient condition, and growth history of adult females on egg size, larval size, and starvation tolerance, growth, and feeding ability of offspring. The fecundity of adult females was strongly dependent on body size. Path analysis revealed that the mother's total length positively affected mean egg diameter, meaning that large females spawned large eggs. In contrast, the relative growth rate of adult females negatively affected egg diameter. Egg diameters positively affected both notochord length and yolk sac volume of the larvae at hatching. Under starvation conditions, notochord length at hatching strongly and positively affected days of survival at 14 degrees C but not at 9 degrees C. Under adequate food conditions (1000 rotifers L-1), the notochord length of larvae 5 days after hatching positively affected feeding rate, implying that large larvae have high feeding ability. In addition, the mean growth rate of larvae between 0 and 15 days increased with increasing egg diameter under homogenous food conditions, suggesting that larvae hatched from large eggs might have a growth advantage for at least to 15 days after hatching. In marbled sole, these relationships (i.e., mother's body size-egg size-larval size-larval resistance to starvation-larval feeding ability) may help explain recruitment variability. (c) 2007 Elsevier B.V All rights reserved.
  • Mitsuhiro Nakaya, Tetsuya Takatsu, Mikimasa Joh, Masayasu Nakagami, Toyomi Takahashi
    FISHERIES SCIENCE 73 (1) 112 - 122 0919-9268 2007/02 [Refereed][Not invited]
     
    An investigation was conducted to evaluate the annual variation in potential predation impact (PPI) on larval and juvenile Pseudopleuronectes yokohamae by Crangon uritai in Hakodate Bay using predator-prey size relationships. Laboratory experiments were designed to estimate the favorable prey size of C. uritai through back-calculation of body length (BL) of P. yokohamae from sagittal otolith diameter observed in the stomachs of C. uritai. The most favorable prey-predator size ratio (BL of P. yokohamae-total length (TL) of C. uritai) class was 0.15-0.19, and ranged from 0.12-0.31. There was a significant positive correlation between the BL increase of P. yokohamae and the bottom water temperature in the field, such that BLs stagnated during the cold year of 1999 from April to June, and increased during the warm year of 2002. In contrast, no significant correlation was found between the TL increase of C. uritai and the bottom water temperature. Moreover, there were no significant differences in the mean TL of C. uritai between months (April-June) or years (1998-2002). Variation of PPI depended on the relationships between the growth rates of P. yokohamae and bottom water temperature. Therefore, the warm year of 2002 resulted in higher potential predation impact (PPI >= 20), and it was at least 20 days shorter than that of the cold year of 1999. These results suggest that increased bottom water temperature in the nursery area was one of the most important factors for cumulative predation loss.
  • 巣山哲, 森岡泰三, 中屋光裕, 中神正康, 上野康弘
    水産総合研究センター研究報告 173 - 180 1346-9894 2006/03/31 [Not refereed][Not invited]
  • M Joh, T Takatsu, M Nakaya, T Higashitani, T Takahashi
    MARINE BIOLOGY 147 (1) 59 - 69 0025-3162 2005/05 [Refereed][Not invited]
     
    We examined the daily deposition of otolith increments of marbled sole (Pseudopleuronectes yokohamae) larvae and juveniles by rearing experiments, and estimated the growth pattern of wild larvae and juveniles in Hakodate Bay (Hokkaido Island, Japan). At 16&DEG; C, prominent checks (inner checks; ca. 19.8 μ m in diameter) were observed on the centers of sagittae and lapilli extracted from 5-day-old larvae. On both otoliths, distinctive and regular increments were observed outside of the inner checks, and the slopes of regression lines between age and the number of increments (n(i)) (for sagittae: n(i)=0.98xDay-5.90; for lapillus: n(i)=0.96xDay-5.70) did not significantly differ from 1. Inner check formations were delayed at lower temperature, and the inner checks formed 13 days after hatching at 8&DEG; C. Over 80% of larvae, just after their yolk-sac has been absorbed completely (stage C), had inner checks on both their otoliths. On the lapilli, other checks (outer check) formed at the beginning of eye migration (stage G). To validate the daily deposition of increments during the juvenile stage, wild captured P. yokohamae juveniles were immersed in alizarin complexone (ALC)-seawater solutions and reared in cages set in their natural habitat. After 6 days, the mean number of rings deposited after the ALC mark was 5.7. The age-body length relationship of wild P. yokohamae larvae and juveniles caught in Hakodate Bay was divided into three phases. In the larval period, the relationship was represented by a quadratic equation (notochord length = -0.010xAge(2) + 0.682xAge-2.480, r(2)=0.82, P< 0.001), and the estimated instantaneous growth was 0.38 mm day(-1) at 15 days, 0 mm day(-1) at 34 days and -0.12 mm day(-1) at 40 days. The age-body length relationship in the early juvenile stage (< 50 days) and the late juvenile stage (> 50 days) were represented by linear equations (standard length=0.055xAge + 5.722 and standard length = 0.345xAg-9.908, respectively). These results showed that the growth rates in the late larval periods and the early juvenile stage were lower than those in the early larval stage and late juvenile stage; during the slow growth period, energy appears to be directed towards metamorphosis rather than body growth. This study provided the information needed to use otolith microstructure analysis for wild marbled sole larvae and juveniles.
  • N Yoshida, T Takatsu, M Nakaya, M Joh, O Kimura, S Shimizu
    NIPPON SUISAN GAKKAISHI 71 (2) 172 - 177 0021-5392 2005/03 [Not refereed][Not invited]
     
    The crangonid shrimp Crangon uritai is one of the predators for marbled sole Pseudopleuronectes yokohamae juveniles in the coastal area of Hakodate Bay, Japan. The catch efficiencies of Hirota's sledge net (mouth size: 60 cm width and 40 cm height) with a distance wheel for C. uritai and P. yokohamae juveniles were obtained to estimate their accurate population densities. Assuning the catch efficiency of the drop trap (a kind of box type quadrat and 0.5 m(2) covered) to be 100%, the catch efficiency of the sledge net was 36-68% for the shrimp and increased with the shift to small body size cohort and so large female individuals disappeared. In contrast, the catch efficiency for P. yokohamae juveniles was lower than that for the shrimp and fluctuated (8-38%), which would be caused by the high escape ability of large juveniles.
  • M Nakaya, T Takatsu, M Nakagami, M Joh, T Takahashi
    FISHERIES SCIENCE 70 (3) 445 - 455 0919-9268 2004/06 [Refereed][Not invited]
     
    To examine predation on larval and juvenile Pleuronectes yokohamae by Crangon uritai, the spatial distribution and feeding habits of C. uritai were studied. Crangon uritai fed on various prey, including P. yokohamae. Density-dependent predation on juvenile Crangon spp., larval and juvenile gobiid fish Chaenogobius heptacanthus, mysids, and gammarids was observed. The abundance of alternative prey might, therefore, play an important role in reducing the pressure of predation on P. yokohamae. In each year, C. uritai migrated from depths of 10 In in March to depths of 3 m in May. There was a significant negative correlation between the weighted mean depth (WMD) of C. uritai and the weighted mean bottom water temperature (WMBWT). The migration was, therefore, delayed in the cold spring, during which C. uritai occupied depths of approximately 10 m. In contrast, no significant correlation was found between the WMD of P. yokohamae and WMBWT Settlement of P. yokohamae began at depths of 15 m, mainly during early April. The spatial distributions of these two organisms show greater overlap during the cold spring, resulting in increased opportunities for predation.
  • Mitsuhiro Nakaya, Tetsuya Takatsu, Masayasu Nakagami, Mikimasa Joh, Toyomi Takahashi
    Suisanzoshoku 日本水産増殖学会 = Japan Aquaculture Society 52 (2) 121 - 128 0371-4217 2004 [Refereed][Not invited]
     
    During the spring in the coastal waters of Hakodate Bay the shrimp Crangon uritai is the predominant predator on larval and juvenile marbled sole, Pleuronectes yokohamae. To investigate the prey preferences of C. uritai, P. yokohamae and two other prey species, a mysid Nipponomysis sp. and a gammarid Haustorioides japonicus, were used for predation experiments. Among the three prey organisms studied, Nipponomysis sp. was preyed on first, followed by P. yokohamae, and finally H. japonicus. Prey profitability did not differ between P. yokohamae and Nipponomysis sp., though H. japonicus was less profitable. Nipponomysis sp. was larger, more perceptible, and slower to escape than P. yokohamae. A relatively high abundance of mysid Nipponomysis sp. could restrict and reduce predation on P. yokohamae by C. uritai. Gammarid H japonicus was able to escape relatively more easily, because its harder and slippery skin required more handling time than the other prey, possibly making it an undesirable food source for C. uritai. High perceptibility and low escape ability may be the most important factors affecting prey choice of C. uritai.餌種の特性の違いとエビジャコの餌選択の関係を知るために，捕食実験を行った。実験はエビジャコ5個体に対して，マコガレイ稚魚とその他エビジャコの主要な餌であるアミ類の1種Nipponomysis sp.とヨコエビ類の1種ナミノリソコエビを，それぞれ10個体ずつ同時に与えて行った。捕食結果，マコガレイ稚魚はエビジャコにとって処理に時間を要しない餌であるが，底面から離れることがほとんどないため発見されにくく，逃避能力も高いため，Nipponomysis sp.よりも捕食されにくい餌であった。ナミノリソコエビはマコガレイ稚魚に比べて小型であるが，捕獲してから処理に時間を要するため，最も捕食されにくい餌であった。このことからマコガレイ稚魚よりも相対的に目立ちやすく，逃避能力が低く，かつ処理に時間を要しない餌（アミ類）の存在は，エビジャコによるマコガレイ稚魚の被食頻度を低下させていると考えられた。
  • Mikimasa Joh, Tetsuya Takatsu, Mitsuhiro Nakaya, Kazuo Imura, Tomomi Higashitani
    Suisanzoshoku 日本水産増殖学会 51 (2) 227 - 228 0371-4217 2003 [Refereed][Not invited]
     
    To estimate the live body length of marbled sole larvae from a preserved specimen, the notochord-length (NL) shrinkage due to preservation was investigated. In 5% formalin solution, significant shrinkage was observed in all NL classes after 30 days and 1.5 years preservation (5.2% and 6.3% in the 2.8-5.0 mm class; 3.3% and 3.8% in the 5.1-7.0 mm class; 3.8% and 4.1% in the 7.0-7.7 mm class, respectively) . In 90% ethanol solution, significant shrinkage was observed only in the 2.8-5.0 mm NL class after both durations (4.9% and 6.3%, respectively) . In both preservatives, the influence of preservation on shrinkage was stronger for small larvae than for large ones.
  • Spatial and temporal distribution of larval and juvenile marbled sole Pleuronectes yokohamae in Hakodate Bay.
    M Nakagami, T Takatsu, M Nakaya, T Takahashi
    水産海洋研究 65 (3) 85 - 93 2001 [Refereed][Not invited]

MISC

Books etc

  • 魚に魅せられた北大の研究者たち 
    海文堂出版 2023/10 (ISBN: 9784303800116) 97p
  • 水産科学・海洋環境科学実習
    中屋 光裕, 髙津 哲也 (Contributor魚類・ベントス測定)
    海文堂 2019
  • 練習船による水産科学・海洋環境科学実習
    中屋光裕, 髙津哲也 (Contributor魚類・ベントス測定)
    五稜出版社 2016

Presentations

  • 津軽海峡周辺におけるキアンコウの初期生態  [Invited]
    高偉峰, 中屋光裕, 高津哲也, 竹谷裕平, 鈴木亮, 野呂恭成
    第49回北洋研究シンポジウムー亜寒帯域における魚類の初期生活史研究と加入量予測の現状  2019/09
  • ホッケ生活史初期の成長  [Invited]
    河村眞美, 中屋光裕, 高津哲也, 巣山哲, 鈴木祐太郎, 高畠信一
    第49回北洋研究シンポジウムー亜寒帯域における魚類の初期生活史研究と加入量予測の現状  2019/09

Association Memberships

  • JAPANESE SOCIETY OF FISHERIES OCEANOGRAPHY   JAPAN AQUACULTURE SOCIETY   THE JAPANESE SOCIETY OF FISHERIES SCIENCE   

Research Projects

  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2021/04 -2024/03 
    Author : 高津 哲也, 中屋 光裕
     
    本研究の目的は，産業重要種であるマダラGadus macrocephalusの野外採集仔魚から「母親効果」を検出し，母親効果を考慮した漁獲への変更が，資源量回復にどの程度貢献するのか解明することである。また，環境変動が資源量変動におよぼす影響も同時解析することで，母親効果を利用した資源保護策の有効性も明らかにする． まず，これまでのマダラの親魚量と着底期までの仔稚魚の生残率の関係を明らかにするために，主要産卵場の一つである青森県陸奥湾の1989年から2019年までの両者の豊度の間で累積的生残率を推定し，その年変動を明らかにした．その結果，累積的生残率は仔魚期の前半にあたる2月下旬から3月中旬の水温が高いほど高く，仔魚期の後半と稚魚期にあたる3月下旬から5月下旬の水温が7.6℃で最大値を示すことを明らかにした。水温はそれぞれ全変動の23%と22%を説明した。 次に，孵化仔魚の耳石輪紋に形成される孵化チェック（通常より太い日周輪）の大きさが，卵径に比例するか明らかにするために，2022年1月に津軽海峡で採集した成熟魚を用いて人工授精・飼育を行い，孵化直前の卵と孵化直後の仔魚を得た。また成熟魚の体長や年齢，栄養状態が卵径におよぼす影響を明らかにするために，耳石による年齢査定と魚体測定を行った。 野外採集仔魚は2021年4月と2022年2月に，主要産卵場である青森県陸奥湾でプランクトンネットによる採集を行った。同時に物理環境データと，仔魚の餌生物であるかいあし類ノープリウスの採集を行った。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2020/04 -2023/03 
    Author : 山本 潤, 足立 亨介, 柳本 卓, 中屋 光裕
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2016/04 -2019/03 
    Author : TAKATSU Tetsuya, NAKAYA Mitsuhiro
     
    To clarify survival processes in Japan Pacific Stock (JPS) of walleye pollock Gadus chalcogrammus, maternal effect and somatic condition were investigated in Funka Bay in 2016-2018. No annual difference in hatch-check diameter on larval otolith derived from maternal was observed between three year-classes. Notochord lengths in late February and early March were small in low water temperature years. The 75-77% of settled juveniles collected in mid-June have settlement checks around their otoliths and settled fork-lengths were estimated at 67.2-69.0 mm from the relationship between fork length and otolith diameter. Feeding intensities and somatic conditions of juveniles decreased in Tsugaru Warm Current water from August to October, then those recovered with water temperature decreasing after October. Annual fluctuations of somatic condition and growth rate might influence the recruitment of JPS.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2016/04 -2019/03 
    Author : YAMAMOTO Jun, SAKURAI Yasunori
     
    The present study examined the first food of Todarodes pacificus paralarvae by assuming that the paralarvae consume microorganisms which were produced by biological loop. The bacterial profile of the egg masses which were obtained through natural spawning in a large experimental tank was similar to those of the nidamental gland (the secretary organ of adult). This result suggested that the adhering bacteria might have originated from indigenous or symbiotic bacteria of the nidamental gland. While， metagenome analysis (CI1 and 16S-rRNA) on the gut contents which were extract from wild paralarvae by Laser Micro-dissection showed that the gut contents were consist of many species of eukaryote and prokaryote (the results on eukaryotic results may require additional testing). The result suggested paralarvae intake microorganism called “detritus”.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2013/04 -2016/03 
    Author : Takatsu Tetsuya, HOSHI NAOKI, NAKAYA MITSUHIRO, ISODA YUTAKA, INAGAKI YUTA, KANO YOTA, NAKATANI TOSHIKUNI, SUZUKI KOTA, TAMURA JUN'ICHI
     
    To clarify the occurrence mechanism of strong-year classes in flathead flounder Hippoglossoides dubius, transport process of pelagic larvae and juveniles and settled area of juveniles were investigated in Funka Bay and its vicinity in 2013-2015 FY. In May 2013, late pelagic stage larvae were collected with MOHT- and FMT-nets around the area off Muroran City located at the north-east end of the bay, but no larva was collected in 2014-2015. It might be important for the recruitment success in the 2013 year-class that the clockwise vortex off Muroran captured the larvae was retained by the predominantly northwesterly winds. In this vortex, suitable water temperature (4.1-5.6 degrees Celsius) and prey abundance were observed for larval growth. In contrast in August-December through 2013-2015, no settled juvenile was collected on the sea floor with an otter trawl net in and out of the bay with <98-m and 95-354-m bottom depths, respectively.

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