Researcher basic information

• 博士（獣医学）, 北海道大学大学院

• https://researchmap.jp/n-ken?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201301085531524663

• 80625898

• 201301085531524663

• 臨床獣医学

• Life Science, Veterinary medical science

• Bachelor's degree program, School of Veterinary Medicine
• Doctoral (PhD) degree program, Graduate School of Veterinary Medicine

Research activity information

• Sep. 2012, 平成24年度日本小動物獣医学会地区学会長賞
  中村健介
• Sep. 2011, 平成23年度日本小動物獣医学会北海道獣医師会長賞
  中村健介
• Mar. 2011, 第151回日本獣医学会学術集会大会長賞
  中村健介
• Sep. 2010, 平成22年度日本小動物獣医学会地区学会長賞
  中村健介
• Sep. 2009, 平成21年度日本小動物獣医学会地区学会長賞
  中村健介
• Sep. 2007, 平成19年度日本小動物獣医学会地区学会長賞
  中村健介

• Deep-learning-based automatic liver segmentation using computed tomography images in dogs
  Seungyeon Lee; Genya Shimbo; Nozomu Yokoyama; Kensuke Nakamura; Ren Togo; Takahiro Ogawa; Miki Haseyama; Mitsuyoshi Takiguchi
  Frontiers in Veterinary Science, 12, Frontiers Media SA, 21 Oct. 2025
  Scientific journal, Introduction
  
  Deep learning-based automated segmentation has significantly improved the efficiency and accuracy of human medicine applications. However, veterinary applications, particularly canine liver segmentation, remain limited. This study aimed to develop and validate a deep learning model based on a 3D U-Net architecture for automated liver segmentation in canine abdominal computed tomography (CT) scans.
  
  Methods
  
  A total of 221 canine abdominal CT scans were analyzed, comprising 159 cases without hepatic masses and 62 cases with hepatic masses. The model was trained and evaluated using two separate datasets: one containing cases without hepatic masses (Experiment 1) and the other combining cases with and without hepatic masses (Experiment 2).
  
  Results
  
  Both experiments demonstrated high segmentation performance, achieving mean Dice similarity coefficients of 0.926 (Experiment 1) and 0.929 (Experiment 2).
  
  Discussion
  
  The manual and predicted liver volumes showed excellent agreement, highlighting the potential clinical applicability of this approach.
• Potential human health risk of carbapenem-non-susceptible Pseudomonas aeruginosa from companion animals.
  Jirachaya Toyting-Hiraishi; Toyotaka Sato; Mana Tohyama; Taro Fujino; Kaho Okada; Kazuyoshi Sasaoka; Nozomu Yokoyama; Kana Torii; Akio Suzuki; Yuzo Tsuyuki; Kensuke Nakamura; Mitsuyoshi Takiguchi; Motohiro Horiuchi
  The Journal of antimicrobial chemotherapy, 19 Sep. 2025, [International Magazine]
  English, Scientific journal, BACKGROUND AND OBJECTIVES: The close bond between companion animals and humans may accelerate the spread of antimicrobial-resistant bacteria. Pseudomonas aeruginosa, an opportunistic pathogen in both, poses a public health threat due to antimicrobial resistance (AMR) and diverse virulence factors. However, One Health-based comparison remains limited. This study investigated the current AMR status and molecular characteristics of P. aeruginosa in companion animals in Japan to assess potential human health risks. METHODS: We examined 197 P. aeruginosa clinical isolates from companion animals [dogs (n = 99) and cats (n = 98)] across Japan in 2024. Antimicrobial susceptibility to human clinical antibiotics was evaluated. In carbapenem-non-susceptible isolates, multilocus sequence typing and detection of resistance genes and virulence factors were performed. RESULTS: Ciprofloxacin (20.3%) and piperacillin (10.7%) showed the highest resistance rates, with 5.6% of isolates being multidrug-resistant. Carbapenem resistance rates were 6.1% for imipenem and 1.0% for meropenem. Thirty-five isolates (17.8%) exhibited carbapenem non-susceptibility but remained susceptible to cefepime, ciprofloxacin, or amikacin. Of 27 identified sequence types, 20 (77.1% of carbapenem-non-susceptible isolates) were known in humans, including two high-risk clones (ST233 and ST298; 8.6%) reported for the first time in Japanese companion animals. These isolates carried mutations in efflux pump-related genes and multiple virulence factors. One showed close genetic relatedness to a human isolate, suggesting possible interspecies transmission. CONCLUSIONS: Our findings highlight the potential cross-species transmission risk of antimicrobial-resistant P. aeruginosa. Identification of shared high-risk clones with multiple virulence factors emphasizes the need for continuous vigilance and actions within the One Health framework.
• Diagnosis of congestive heart failure by combining echocardiography and blood biomarkers in cats with cardiomyopathy.
  T Shimoda; T Osuga; K Nakamura
  Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology, 60, 70, 78, Aug. 2025, [International Magazine]
  English, Scientific journal, INTRODUCTION/OBJECTIVES: In cats with cardiomyopathy, echocardiographic indices and blood biomarkers such as atrial natriuretic peptide (ANP), cardiac troponin I (cTnI), and N-terminal pro-B-type natriuretic peptide (NTproBNP) are useful in diagnosing congestive heart failure (CHF). However, the improvement in diagnostic accuracy by combining these indicators remains unclear. ANIMALS, MATERIALS AND METHODS: Twenty-six cats diagnosed with cardiomyopathy using echocardiography were included in this study. They were classified into CHF and non-CHF groups according to the presence of pulmonary oedema or pleural effusion and response to heart failure treatment. The discriminative ability of each index for the presence of CHF was evaluated using a receiver operating characteristic curve. RESULTS: The left atrial-to-aortic root ratio (LA/Ao) had the highest area under the curve of 0.952 (95% confidence interval [CI]: 0.878-1.0), followed by ANP 0.915 (95% CI: 0.808-1.0), cTnI 0.861 (95% CI: 0.722-0.999), and NTproBNP 0.830 (95% CI: 0.672-0.988). Adding NTproBNP and ANP to the LA/Ao did not improve the discriminative ability. Limited by the combination of blood biomarkers, ANP and cTnI showed the highest discriminative ability. STUDY LIMITATIONS: Following were the limitations to this study: lack of standardised medication, inability to calculate true correlation for NTproBNP values >1500 pmol/L, marked difference in disease severity between groups, inclusion of various cardiomyopathy types, reliance solely on LA/Ao for left atrial size assessment, and small sample size. CONCLUSIONS: This study indicates that if LA/Ao can be measured accurately, the significance of measuring cardiac biomarkers for diagnosing CHF in cats with cardiomyopathy is low.
• Single Case of a Cat Suspected of Having Congenital Biliary Dilatation Associated with Pancreaticobiliary Maljunction.
  Shinsuke Fujimoto; Shidow Torisu; Keisuke Nakatani; Ryuta Amiya; Yuho Hosaka; Yasuyuki Kaneko; Kensuke Nakamura; Mika Tanabe
  Journal of the American Animal Hospital Association, 61, 3, 50, 55, 01 May 2025, [International Magazine]
  English, Scientific journal, A 1 yr old spayed female Scottish fold cat was referred for further investigation because of loss of appetite and markedly elevated liver enzyme activities. Significant biliary dilatation and positive bile culture were documented, raising suspicion for cholangitis. A strictured/stenotic duodenal papilla was noted on laparotomy, with retrograde cholangiopancreatography documenting the main pancreatic duct joined with the common bile duct outside the duodenal wall. In addition, pancreatic enzyme activity in the bile was high, suggesting that pancreatic juice was flowing back into the biliary tract. These findings are similar to those of pancreaticobiliary maljunction observed in humans. Duodenal papillotomy improved the excretion of bile and pancreatic juice, resolving both the bile duct dilatation and elevated liver enzyme activities. To our knowledge, this is the first report demonstrating the association of pancreaticobiliary maljunction with the development of cholangitis associated with biliary dilatation in cats. In clinical practice, when cholangitis with severe biliary dilatation is observed in a young cat, the association with pancreaticobiliary maljunction should be considered.
• Evaluation of the course of improvement with molnupiravir treatment for feline infectious peritonitis.
  Shino Yoshida; Mei Sugawara-Suda; Kazuyoshi Sasaoka; Noboru Sasaki; Nozomu Yokoyama; Kensuke Nakamura; Keitaro Morishita; Sangho Kim; Takafumi Sunaga; Mitsuyoshi Takiguchi
  The Canadian veterinary journal = La revue veterinaire canadienne, 66, 5, 546, 554, May 2025, [International Magazine]
  English, Scientific journal, OBJECTIVE: To clarify the clinical course during molnupiravir treatment for feline infectious peritonitis (FIP). ANIMALS AND PROCEDURE: Cats diagnosed with FIP and treated with molnupiravir at Hokkaido University Veterinary Teaching Hospital (Sapporo, Hokkaido, Japan) were retrospectively reviewed. RESULTS: Eleven cats were eligible for inclusion. Six cats had effusive FIP and 5 had non-effusive FIP. In noneffusive cases, 2 cats had neurological abnormalities at diagnosis, whereas 1 additional cat developed neurological signs during treatment. The median initial dosage of molnupiravir was 13.0 mg/kg (range: 10.0 to 15.0 mg/kg), PO, q12h. One cat died after 11 d and the remaining 10 cats completed an 84-day course of treatment. All neurological cases were given dosage increases, extended treatment duration, or both. The median final dosage of molnupiravir in non-neuro-FIP cases was 13.1 mg/kg (range: 10.0 to 15.0 mg/kg), PO, q12h, whereas dosages in neuro-FIP cases were 15.0, 15.2, and 17.2 mg/kg, PO, q12h in the 3 affected cats, respectively. In non-neurological cases, dysrexia, lethargy, and high serum amyloid A were resolved within 15 d. Total follow-up duration ranged from 175 to 362 d. No relapses were observed. CONCLUSION AND CLINICAL RELEVANCE: Monitoring responses to molnupiravir treatment requires observing clinical signs and conducting clinicopathological evaluations, including acute-phase protein evaluation.
• Transfemoral vein approach for balloon pulmonary valvuloplasty in a 1.2- kg dog.
  K Kadowaki; N Miyashita; K Nakamura; T Mori
  Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology, 59, 133, 138, 04 Apr. 2025, [International Magazine]
  English, Scientific journal, A 10-month-old, 1.2- kg, unspayed female Maltese dog presented with the chief complaint of a heart murmur. Echocardiography revealed a transpulmonary velocity of 5.28 m/s (estimated pressure gradient: 112 mmHg) and severe pulmonary valve stenosis with an estimated pulmonary valve annulus diameter of 7.2 mm. Given the dog's small size, a balloon pulmonary valvuloplasty via the femoral vein was considered. The pulmonary valve annulus was dilated thrice using a 10-mm balloon (balloon-to-annulus ratio: 1.39). After the balloon pulmonary valvuloplasty, right ventricular systolic pressure decreased from 125 to 78 mmHg. The interventional procedure was completed without serious complications. As of postoperative day 149, the maximum transpulmonary velocity decreased to 2.43 m/s (estimated pressure gradient: 24 mmHg), recurrence of pulmonary valve stenosis was not observed, and the patient was doing well clinically. In conclusion, an approach performed via the femoral vein in this 1.2-kg dog resulted in a successful procedure without significant complications.
• Pre-operative left atrial size and functions are predictors of left atrial reverse remodelling after mitral valvuloplasty for myxomatous mitral valve disease in dogs.
  S Kawamoto; K Nakamura; T Mori; T Wada; A Fujiwara; M Takiguchi
  Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology, 57, 47, 57, Feb. 2025, [International Magazine]
  English, Scientific journal, OBJECTIVES: The objective of this study was to determine alterations in left atrial size, function, and determinants of left atrial reverse remodelling (LARR) after mitral valvuloplasty (MVP) in dogs with myxomatous mitral valve disease. ANIMALS: Thirty-seven dogs undergoing MVP were included in this study. MATERIALS AND METHODS: Echocardiographic parameters, including left atrial (LA) strain, were evaluated at baseline, during the early postoperative period (within two weeks), and at one and three months postoperatively. Patients were divided into two groups based on the attainment of LARR, defined as a left atrial-to-aortic root ratio (LA:Ao) < 1.6 at three months. The predictors of LARR were analysed using univariate logistic regression analysis. RESULTS: Left atrial-to-aortic root ratio significantly decreased in the early postoperative period (median: 1.68; lower and upper quartiles: 1.54-1.92) compared to that in baseline (2.41; 2.00-2.80), with a further significant reduction observed at three months (1.46; 1.34-1.63). Left atrial reverse remodelling was achieved in 27 (73%) patients. Baseline left atrial-to-aortic root ratio was higher, and peak atrial longitudinal strain and peak atrial contraction strain (PACS) were lower in the non-LARR group than in the LARR group. Among the baseline parameters, PACS was the strongest predictor of LARR (area under the curve: 0.837). CONCLUSIONS: Following MVP, the LA size decreased in the early postoperative period and continued to decrease over three months of follow-up. A low PACS score was the most reliable predictor of inadequate LARR. Further studies are necessary to determine the relationship between LARR, prognosis, and the optimal timing for surgical intervention.
• Percutaneous transcatheter right atrial stent placement for recurrent cor triatriatum dexter following initial surgical excision of right intra-atrial membrane in a dog.
  K Kadowaki; H Tomita; T Wada; Y Miki; A Fujiwara; K Nakamura; T Mori
  Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology, 55, 9, 14, Oct. 2024, [International Magazine]
  English, Scientific journal, A four-year-old spayed female Shiba Inu dog weighing 6.1 kg presented with ascites. Cor triatriatum dexter (CTD) without any other concurrent cardiovascular anomalies was diagnosed using echocardiography. The ostium of the abnormal membrane dividing the right atrium into two abnormal chambers was surgically excised under cardiopulmonary bypass. All clinical abnormalities were resolved after surgery. However, seven months later, ascites and CTD recurred. A balloon-expandable stent was placed at the site of recurrence, which improved caudal venous return to the right ventricle. After the second procedure, ascites were resolved, and the dog remained asymptomatic for 18 months without complications. There are previous reports of successful surgical resection of the CTD and of stenting in recurrent CTD following balloon dilation in dogs. However, to the authors' knowledge, no previous reports have described recurrent CTD in dogs after surgical resection of the CTD. In this case, as with recurrent CTD following balloon dilation, stent placement at the site of the CTD can be a viable treatment option when the abnormal membrane recurs.
• ラブラドール・レトリーバーのコロニーで集団発生したサルファ剤過敏症とCYPB5Rの遺伝子型頻度解析
  岸原 果子; 横山 望; 山崎 淳平; 笹岡 一慶; 森下 啓太郎; 中村 健介; 宮原 和郎; 近藤 厚; 滝口 満喜; 高橋 徹
  北海道獣医師会雑誌, 68, 8, 314, 314, (公社)北海道獣医師会, Aug. 2024
  Japanese
• 気管支拡張症の原因として気管支軟骨の低形成が疑われた2頭のアメリカン・コッカー・スパニエル
  川上 侑記; 中村 健介; 菅原 芽伊; 新坊 弦也; 山根 由久; 細谷 謙次; 岡田 一喜; 賀川 由美子; 滝口 満喜
  北海道獣医師会雑誌, 68, 8, 321, 321, (公社)北海道獣医師会, Aug. 2024
  Japanese
• 部分肺静脈還流異常により右心拡大を呈した犬の1例
  安田 理紗; 中村 健介; 新坊 弦也; 横山 望; 滝口 満喜
  北海道獣医師会雑誌, 68, 8, 323, 323, (公社)北海道獣医師会, Aug. 2024
  Japanese
• Case report: Echinococcus multilocularis infection in a dog showing gastrointestinal signs in Hokkaido, Japan
  Izumi Kida; Naoki Hayashi; Nozomu Yokoyama; Noriyuki Nagata; Kazuyoshi Sasaoka; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Hirokazu Kouguchi; Kinpei Yagi; Ryo Nakao; Mitsuyoshi Takiguchi; Nariaki Nonaka
  Frontiers in Veterinary Science, 11, Frontiers Media SA, 13 Jun. 2024
  Scientific journal, Echinococcus multilocularis is a cestode that causes human alveolar echinococcosis, a lethal zoonotic disease distributed in the northern hemisphere. The life cycle of this parasite is maintained in nature by voles as intermediate hosts and foxes as definitive hosts in Hokkaido, Japan. Although dogs are also susceptible to the parasite, the infection has been considered typically asymptomatic. We report the detection of E. multilocularis eggs in the diarrheal feces of a dog with chronic gastrointestinal signs, which disappeared after anthelmintic treatment. The mitochondrial genome sequence constructed by sequencing of the overlapping PCRs using DNA from the eggs was identical to the most predominant haplotype previously reported in red foxes in Hokkaido. This case highlights that Echinococcus infection should be considered as a differential diagnosis for diarrheal dogs in the disease endemic areas. Further efforts are needed to accumulate parasite genotypes in domestic dogs as well as humans to assess the risk of human infection from dogs.
• Urinary steroid profiling using liquid chromatography-tandem mass spectrometry for the diagnosis of canine Cushing's syndrome.
  N Nagata; H Sawamura; Y Ikenaka; K Morishita; K Hosoya; N Sasaki; K Nakamura; M Takiguchi
  Veterinary journal (London, England : 1997), 106151, 106151, 29 May 2024, [International Magazine]
  English, Scientific journal, Serum cortisol measurements by chemiluminescence enzyme immunoassay (CLEIA) are widely used to diagnose hypercortisolism (HC) or Cushing's syndrome in dogs. However, they are associated with problems such as the need for multiple blood collections under stressful conditions or cross-reactivity between hormones. Therefore, a less invasive and more accurate diagnostic method is required. This study aimed to develop a urinary steroid profile analysis method using liquid chromatography-tandem mass spectrometry (LC/MS/MS) and to evaluate its clinical usefulness. Sixty-five healthy dogs and 38 dogs with suspected HC were included in the study. Using LC/MS/MS, the levels of 11 steroid hormones in the urine were determined. We established the upper limit of the reference interval for each urinary steroid-to-creatinine ratio and evaluated their diagnostic performances. The levels of the five steroid hormones were significantly higher in the 14 dogs with HC than in the 24 dogs with mimicking HC and 65 healthy dogs. The urinary corticosterone-to-creatinine ratio showed the highest diagnostic accuracy (area under the curve, 0.96). A significant correlation was seen between urinary cortisol concentrations measured by LC/MS/MS and CLEIA (rs = 0.88, P <0.001), although the CLEIA measurements were significantly higher than the LC/MS/MS measurements (P <0.001). LC/MS/MS-based urinary steroid profiles are a promising tool for diagnosing canine HC.
• A de novo nonsense variant in the DMD gene associated with X-linked dystrophin-deficient muscular dystrophy in a cat.
  Nozomu Yokoyama; Yuki Matsumoto; Takahisa Yamaguchi; Kazuki Okada; Ryohei Kinoshita; Genya Shimbo; Hisashi Ukawa; Ryuga Ishii; Kensuke Nakamura; Jumpei Yamazaki; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 13 Apr. 2024, [International Magazine]
  English, Scientific journal, BACKGROUND: X-linked dystrophin-deficient muscular dystrophy (MD) is a form of MD caused by variants in the DMD gene. It is a fatal disease characterized by progressive weakness and degeneration of skeletal muscles. HYPOTHESIS/OBJECTIVES: Identify deleterious genetic variants in DMD by whole-genome sequencing (WGS) using a next-generation sequencer. ANIMALS: One MD-affected cat, its parents, and 354 cats from a breeding colony. METHODS: We compared the WGS data of the affected cat with data available in the National Center for Biotechnology Information database and searched for candidate high-impact variants by in silico analyses. Next, we confirmed the candidate variants by Sanger sequencing using samples from the parents and cats from the breeding colony. We used 2 genome assemblies, the standard felCat9 (from an Abyssinian cat) and the novel AnAms1.0 (from an American Shorthair cat), to evaluate genome assembly differences. RESULTS: We found 2 novel high-impact variants: a 1-bp deletion in felCat9 and an identical nonsense variant in felCat9 and AnAms1.0. Whole genome and Sanger sequencing validation showed that the deletion in felCat9 was a false positive because of misassembly. Among the 357 cats, the nonsense variant was only found in the affected cat, which indicated it was a de novo variant. CONCLUSION AND CLINICAL IMPORTANCE: We identified a de novo variant in the affected cat and next-generation sequencing-based genotyping of the whole DMD gene was determined to be necessary for affected cats because the parents of the affected cat did not have the risk variant.
• Interventricular inflow time difference assessed by dual pulsed-wave Doppler echocardiography in dogs with myxomatous mitral valve disease
  T. Morita; K. Nakamura; T. Osuga; S. Kawamoto; M. Takiguchi
  Journal of Veterinary Cardiology, 51, 24, 34, Elsevier BV, Feb. 2024, [International Magazine]
  English, Scientific journal, INTRODUCTION: Interventricular inflow time difference (IVID), which is defined as the time interval between the opening of the mitral valve and the opening of the tricuspid valve, hold prognostic value in human patients with heart failure. Few reports regarding IVID are available in dogs. ANIMALS: Ninety dogs with myxomatous mitral valve disease (MMVD) and 47 dogs without heart disease. Dogs with MMVD received unstandardized therapy based on the stage of disease. MATERIALS AND METHODS: This was a prospective cohort study. Dogs were classified into two groups based on IVID: tricuspid opening preceding mitral opening (TOP) and mitral opening preceding tricuspid opening (MOP). The potential influence of the MOP group at enrollment on the primary outcome (cardiac-related death) was determined by Kaplan-Meier analysis. RESULTS: Almost all dogs without heart disease (97.9%) were classified in the TOP group. Twenty-nine dogs (32.2%) were classified in the MOP group. Left heart size, transmitral early diastolic inflow velocity, and right ventricular Tei index were significantly greater in the MOP group compared to those in the TOP group. Cardiac-related death were observed in 34 dogs (40.5%). The dogs in the MOP group at initial examination had shorter survival times than those in the TOP group (586 days vs. >1,831 days; 95% confidence interval, 237-714 days vs. 1,037 days to >1,831 days; P<0.001). CONCLUSIONS: Interventricular inflow time difference is potentially useful for prognostic assessments in dogs with MMVD. Further prospective studies that quantify the repeatability and influence of therapy on IVID are needed.
• Adrenocortical hypoperfusion detected by contrast-enhanced ultrasound in a dog with trilostane-induced hypoadrenocorticism.
  N Nagata; K Aoshima; K Nakamura; M Takiguchi
  The Journal of small animal practice, 64, 11, 722, 726, Nov. 2023, [International Magazine]
  English, A 12-year-old neutered male Chihuahua dog was diagnosed with pituitary-dependent hypercortisolism and treated with trilostane. Eighty-nine days later, the dog showed lethargy accompanied by hyponatraemia and hyperkalaemia. Hypoadrenocorticism due to trilostane was suspected, but the result of the adrenocorticotropic hormone stimulation test was not conclusive. Contrast-enhanced ultrasound showed loss of adrenocortical blood flow in both adrenal glands, indicating adrenocortical hypoperfusion and isolated hypoadrenocorticism. Treatment with fludrocortisone acetate improved the condition and electrolyte abnormalities. Thirteen months later, the dog showed alopecia, and an adrenocorticotropic hormone stimulation test revealed increased cortisol concentration, indicating hypercortisolism recurrence. The dog died due to progressive deterioration 22 months after the initial presentation. Post-mortem examination revealed focally extensive necrosis with marked calcification in the parenchyma of the adrenal glands and regeneration of the cells in the zona fasciculata with severe fibrosis. Adrenocortical hypoperfusion detected by contrast-enhanced ultrasound can support the diagnosis of adrenal necrosis and hypoadrenocorticism.
• 犬の腎うっ血の評価におけるShear wave elastographyの有用性の検討
  塩原 希; 中村 健介; 横山 望; 森下 啓太郎; 滝口 満喜
  北海道獣医師会雑誌, 67, 8, 316, 316, (公社)北海道獣医師会, Aug. 2023
  Japanese
• 拘束型心筋症に伴う左室内血栓により大動脈血栓塞栓に陥った猫の1例
  立花 圭; 中村 健介; 塩原 希; 川上 侑記; 滝口 満喜
  北海道獣医師会雑誌, 67, 8, 318, 318, (公社)北海道獣医師会, Aug. 2023
  Japanese
• Diverse genome-wide DNA methylation alterations in canine hepatocellular tumours.
  Yu Asari; Jumpei Yamazaki; Oo Thandar; Tamami Suzuki; Keisuke Aoshima; Kyosuke Takeuchi; Ryohei Kinoshita; Sangho Kim; Kenji Hosoya; Teita Ishizaki; Yumiko Kagawa; Jaroslav Jelinek; Shoko Yokoyama; Noboru Sasaki; Hiroshi Ohta; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Veterinary medicine and science, 22 Jul. 2023, [International Magazine]
  English, Scientific journal, BACKGROUND: Canine hepatocellular tumours (HCTs) are common primary liver tumours. However, the exact mechanisms of tumourigenesis remain unclear. Although some genetic mutations have been reported, DNA methylation alterations in canine HCT have not been well studied. OBJECTIVES: In this study, we aimed to analyse the DNA methylation status of canine HCT. METHODS: Tissues from 33 hepatocellular carcinomas, 3 hepatocellular adenomas, 1 nodular hyperplasia, 21 non-tumour livers from the patients and normal livers from 5 healthy dogs were used. We analysed the DNA methylation levels of 72,367 cytosine-guanine dinucleotides (CpG sites) in all 63 samples. RESULTS AND CONCLUSIONS: Although a large fraction of CpG sites that were highly methylated in the normal liver became hypomethylated in tumours from most patients, we also found some patients with less remarkable change or no change in DNA methylation. Hierarchical clustering analysis revealed that 32 of 37 tumour samples differed from normal livers, although the remaining 5 tumour livers fell into the same cluster as normal livers. In addition, the number of hypermethylated genes in tumour livers varied among tumour cases, suggesting various DNA methylation patterns in different tumour groups. However, patient and clinical parameters, such as age, were not associated with DNA methylation status. In conclusion, we found that HCTs undergo aberrant and diverse patterns of genome-wide DNA methylation compared with normal liver tissue, suggesting a complex epigenetic mechanism in canine HCT.
• An inflammatory bowel disease-associated SNP increases local thyroglobulin expression to develop inflammation in miniature dachshunds
  Yong Bin Teoh; Jing-Jing Jiang; Takeshi Yamasaki; Noriyuki Nagata; Toshiki Sugawara; Rie Hasebe; Hiroshi Ohta; Noboru Sasaki; Nozomu Yokoyama; Kensuke Nakamura; Yumiko Kagawa; Mitsuyoshi Takiguchi; Masaaki Murakami
  Frontiers in Veterinary Science, 10, Frontiers Media SA, 14 Jul. 2023
  Scientific journal, Inflammatory colorectal polyp (ICRP) in miniature dachshunds (MDs) is a chronic inflammatory bowel disease (IBD) characterized by granulomatous inflammation that consists of neutrophil infiltration and goblet cell hyperplasia in the colon. Recently, we identified five MD-associated single-nucleotide polymorphisms (SNPs), namely PLG, TCOF1, TG, COL9A2, and COL4A4, by whole-exome sequencing. Here, we investigated whether TG c.4567C&gt;T (p.R1523W) is associated with the ICRP pathology. We found that the frequency of the T/T SNP risk allele was significantly increased in MDs with ICRP. In vitro experiments showed that TG expression in non-immune cells was increased by inducing the IL-6 amplifier with IL-6 and TNF-α. On the other hand, a deficiency of TG suppressed the IL-6 amplifier. Moreover, recombinant TG treatment enhanced the activation of the IL-6 amplifier, suggesting that TG is both a positive regulator and a target of the IL-6 amplifier. We also found that TG expression together with two NF-κB targets, IL6 and CCL2, was increased in colon samples isolated from MDs with the T/T risk allele compared to those with the C/C non-risk allele, but serum TG was not increased. Cumulatively, these results suggest that the T/T SNP is an expression quantitative trait locus (eQTL) of TG mRNA in the colon, and local TG expression triggered by this SNP increases the risk of ICRP in MDs via the IL-6 amplifier. Therefore, TG c.4567C&gt;T is a diagnostic target for ICRP in MDs, and TG-mediated IL-6 amplifier activation in the colon is a possible therapeutic target for ICRP.
• Successful treatment of sclerosing encapsulating peritonitis in a cat using bioresorbable hyaluronate-carboxymethylcellulose membrane after surgical adhesiolysis and long-term prednisolone
  Nozomu Yokoyama; Ryohei Kinoshita; Hiroshi Ohta; Kazuki Okada; Genya Shimbo; Kazuyoshi Sasaoka; Noriyuki Nagata; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Yumiko Kagawa; Mitsuyoshi Takiguchi
  Journal of Feline Medicine and Surgery Open Reports, 9, 2, SAGE Publications, Jul. 2023
  Scientific journal, Case summary
  
  An 8-year-old neutered male domestic shorthair indoor cat was presented with an 8-week history of intermittent vomiting, anorexia and weight loss that had been unresponsive to supportive treatment. Abdominal ultrasound revealed plication of the small intestine and fluid accumulation proximal to the lesion, and a linear foreign body was suspected. An exploratory celiotomy showed cocoon-like encapsulation of the entire intestine. Surgical adhesiolysis and full-thickness biopsy were performed, and histopathologic examination revealed mild thickening of the visceral peritoneum with fibrin deposition, as well as mild neutrophil and lymphocyte infiltration. These findings were compatible with sclerosing encapsulating peritonitis (SEP). The cat recovered well postoperatively and was discharged the next day. Prednisolone was administered for 7 weeks to prevent recurrence of SEP. Five months after surgery, the cat was re-presented with anorexia and chronic vomiting. Based on the clinical examination findings, recurrent SEP was suspected. At the second surgery, surgical adhesiolysis was repeated and a bioresorbable hyaluronate-carboxymethylcellulose membrane was used to cover the serosal surface and thus prevent adhesion formation. Histopathologic findings of the peritoneal biopsy specimen confirmed SEP. Long-term prednisolone treatment (1 mg/kg for the first dose and 0.5 mg/kg every 48 h for maintenance) was administered postoperatively. The cat survived for more than 1239 days without recurrence.
  
  Relevance and novel information
  
  To our knowledge, this is the first report of SEP in a cat with long-term survival. The use of a bioresorbable hyaluronate-carboxymethylcellulose membrane and long-term prednisolone treatment may have prevented short-term and long-term recurrence, respectively, in this case.
• Investigation of the therapeutic effects, predictors, and complications of long-term immunosuppressive therapy in dogs with precursor-targeted immune-mediated anemia.
  Mei Sugawara-Suda; Keitaro Morishita; Yuto Iwanaga; Jumpei Yamazaki; Yumiko Kagawa; Nozomu Yokoyama; Noboru Sasaki; Hiroshi Ohta; Kensuke Nakamura; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 85, 7, 695, 701, 01 Jul. 2023, [Domestic magazines]
  English, Scientific journal, Dogs with precursor-targeted immune-mediated anemia (PIMA) are commonly treated with immunosuppressive therapy, but information on predictors of treatment response and response time is limited. Therefore, we retrospectively investigated predictive factors that influenced the treatment response and duration required to observe a response in dogs with PIMA receiving continuous immunosuppressive therapies for more than 105 days. Of 50 client-owned dogs that developed PIMA, 27 were included in this study, of which 18 were responders and 9 were non-responders to immunosuppressive therapies. Sixteen of the 18 responders responded to treatment within 60 days and the remaining 2 responded at 93 and 126 days, respectively. We found that an erythroid-maturation ratio of <0.17 may be a useful predictor for treatment response. In addition, complications of immunosuppressive therapies were investigated further in 50 dogs. Pancreatitis (n=4) and pneumonia (3) occurred over the entire treatment period, and infections such as abscesses (3) tended to be more common in dogs on an extended period of immunosuppressive therapy. These findings may be helpful when planning for the initial treatment and may provide evidence for informed consent about potential comorbidities throughout the treatment course.
• Evaluation of responses to immunosuppressive therapy in dogs with suspected non‐regenerative immune‐mediated anaemia: 11 cases (2012‐2018)
  K. Morishita; M. Sugawara‐Suda; J. Yamazaki; N. Sasaki; K. Nakamura; H. Ohta; M. Takiguchi
  Journal of Small Animal Practice, Wiley, 07 Apr. 2023
  Scientific journal
• Zoobiquity experiments show the importance of the local MMP9-plasminogen axis in inflammatory bowel diseases in both dogs and patients.
  Takeshi Yamasaki; Noriyuki Nagata; Toru Atsumi; Rie Hasebe; Yuki Tanaka; Izuru Ohki; Shimpei Kubota; Yuta Shinohara; Yong Bin Teoh; Nozomu Yokoyama; Noboru Sasaki; Kensuke Nakamura; Hiroshi Ohta; Takehiko Katsurada; Yoshihiro Matsuno; Shintaro Hojyo; Shigeru Hashimoto; Mitsuyoshi Takiguchi; Masaaki Murakami
  International immunology, 35, 7, 313, 326, 18 Mar. 2023, [International Magazine]
  English, Scientific journal, Using a zoobiquity concept, we directly connect animal phenotypes to a human disease mechanism: the reduction of local plasminogen levels caused by matrix metalloproteinase-9 (MMP9) activity is associated with the development of inflammation in the intestines of dogs and patients with inflammatory bowel disease. We first investigated inflammatory colorectal polyps (ICRPs), which are a canine gastrointestinal disease characterized by the presence of idiopathic chronic inflammation, in Miniature Dachshund (MD), and found 31 missense disease-associated SNPs by whole-exome sequencing. We sequenced them in 10 other dog breeds and found five, PLG, TCOF1, TG, COL9A2, and COL4A4, only in MD. We then investigated two rare and breed-specific missense SNPs (T/T SNPs), PLG: c.477G>T and c.478A>T, and found that ICRPs with the T/T SNP risk-alleles showed less intact plasminogen and plasmin activity in the lesions compared to ICRPs without the risk-alleles but no differences in serum. Moreover, we show that MMP9, which is a NF-κB target, caused the plasminogen reduction and that intestinal epithelial cells expressing plasminogen molecules were colocalized with epithelial cells expressing MMP9 in normal colons with the risk-alleles. Importantly, MMP9 expression in patients with ulcerous colitis or Crohn's disease also colocalized with epithelial cells showing enhanced NF-κB activation and less plasminogen expression. Overall, our zoobiquity experiments showed that MMP9 induces the plasminogen reduction in intestine, contributing to the development of local inflammation and suggesting the local MMP9-plasminogen axis is a therapeutic target in both dogs and patients. Therefore, zoobiquity-type experiments could bring new perspectives for biomarkers and therapeutic targets.
• Prognostic value of left atrial stiffness estimated using echocardiography in dogs with myxomatous mitral valve disease
  T. Osuga; K. Kuroda; T. Morita; N. Sasaki; K. Nakamura; M. Takiguchi
  Journal of Veterinary Cardiology, 45, 15, 26, Feb. 2023
  Scientific journal
• Transcriptome and proteome analysis of dogs with precursor targeted immune-mediated anemia treated with splenectomy.
  Mei Sugawara-Suda; Keitaro Morishita; Osamu Ichii; Takashi Namba; Keisuke Aoshima; Yumiko Kagawa; Sangho Kim; Kenji Hosoya; Nozomu Yokoyama; Noboru Sasaki; Kensuke Nakamura; Jumpei Yamazaki; Mitsuyoshi Takiguchi
  PloS one, 18, 5, e0285415, 2023, [International Magazine]
  English, Scientific journal, Precursor-targeted immune-mediated anemia (PIMA) in dogs is characterized by persistent non-regenerative anemia and ineffective erythropoiesis, and it is suspected to be an immune-mediated disease. Most affected dogs respond to immunosuppressive therapies; however, some are resistant. In this study, we carried out splenectomy as an alternative therapy for refractory PIMA in dogs, and analyzed gene expression levels in the spleen of dogs with or without PIMA and in serum before and after splenectomy. A total of 1,385 genes were found to express differentially in the spleens from dogs with PIMA compared with healthy dogs by transcriptome analysis, of which 707 genes were up-regulated, including S100A12, S100A8, and S100A9 that are linked directly to the innate immune system and have been characterized as endogenous damage-associated molecular patterns. Furthermore, immunohistochemistry confirmed that S100A8/A9 protein expression levels were significantly higher in dogs with PIMA compared with those in healthy dogs. A total of 22 proteins were found to express differentially between the serum samples collected before and after splenectomy by proteome analysis, of which 12 proteins were up-regulated in the samples before. The lectin pathway of complement activation was identified by pathway analysis in pre-splenectomy samples. We speculated that S100A8/9 expression may be increased in the spleen of dogs with PIMA, resulting in activation of the lectin pathway before splenectomy. These findings further our understanding of the pathology and mechanisms of splenectomy for PIMA.
• Resolution of Signs of Epiglottic Retroversion Following Medical Management of Hyperadrenocorticism in a Dog.
  Shota Wada; Kensuke Nakamura
  Journal of the American Animal Hospital Association, 58, 6, 288, 291, 01 Nov. 2022, [International Magazine]
  English, Scientific journal, A 6 yr old spayed female Chihuahua was referred for a 10 mo history of chronic respiratory compromise. Decreased serum thyroxine and thyroid-stimulating hormone concentrations had been confirmed at a primary clinic, but no treatment was initiated. Serum biochemistries revealed elevated alkaline phosphatase and cholesterol concentrations. An adrenocorticotropic hormone-stimulating test revealed elevated preserum and postserum cortisol concentrations. Fluoroscopy revealed marked epiglottic retroversion (ER) during inhalation. Enlarged bilateral adrenal glands were found on abdominal ultrasonography. Based on these findings, ER and hyperadrenocorticism (HAC) were diagnosed and surgical correction of the ER was planned. Trilostane administration was initiated before surgery to reduce the risk of thrombosis due to HAC. Seven days after the initiation of trilostane therapy, clinical signs of chronic respiratory compromise were resolved. The patient had remained clinically stable without recurrence of respiratory compromise for at least 15 mo at the time of this case report. This case suggests that HAC could contribute to the development of clinical signs of ER, which could potentially be successfully controlled by medical treatment of HAC.
• Serum steroid profiling of hepatocellular carcinoma associated with hyperadrenocorticism in dogs: A preliminary study
  Thandar Oo; Noboru Sasaki; Yoshinori Ikenaka; Takahiro Ichise; Noriyuki Nagata; Nozomu Yokoyama; Kazuyoshi Sasaoka; Keitaro Morishita; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Frontiers in Veterinary Science, 9, Frontiers Media SA, 28 Sep. 2022
  Scientific journal, Background
  
  Hepatocellular carcinoma (HCC) is one of the most common primary liver tumors in humans and dogs. Excessive adrenocortical hormone exposure may cause steroid hepatopathy, which may develop into HCC. In our previous study, hyperadrenocorticism (HAC) was a highly concurrent disease in dogs with HCC. Therefore, this study hypothesized that adrenal steroid alterations might be involved in hepatocarcinogenesis and aimed to specify the relationship between HAC and HCC in dogs.
  
  Materials and methods
  
  This study included 46 dogs brought to the Hokkaido University Veterinary Teaching Hospital between March 2019 and December 2020. Owners gave their signed consent for blood collection on their first visit. A total of 19 steroids (14 steroids and 5 metabolites) in the baseline serum of 15 dogs with HCC, 15 dogs with HAC, and 10 dogs with both diseases were quantitatively measured using the developed liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method.
  
  Results
  
  In each group, 11 steroids were detected higher than 50%. The detection rate of steroid hormones did not significantly differ between the groups (p &gt; 0.05). Principle component analysis (PCA) showed that the steroid profiles of the three groups were comparable. Median steroid hormone concentrations were not significantly different between the study diseases (p &gt; 0.05).
  
  Conclusion
  
  The developed LC/MS/MS was useful for measuring steroid hormones. Although it was clear that HAC was concurrent in dogs with HCC, none of the serum steroids was suggested to be involved in HCC.
• 磁気共鳴スペクトロスコピーは犬の起源不明髄膜脳脊髄炎の予後指標となり得るか
  山口 敬央; 笹岡 一慶; 新坊 弦也; 山根 由久; 永田 矩之; 横山 望; 森下 啓太郎; 佐々木 東; 中村 健介; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 287, 287, (公社)北海道獣医師会, Aug. 2022
  Japanese
• 洞停止を認めた咳嗽失神の犬の1例
  幸野 瑠美; 中村 健介; 笹岡 一慶; 塩原 希; 小幡 祐路; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 284, 284, (公社)北海道獣医師会, Aug. 2022
  Japanese
• 不完全型房室中隔欠損症の外科的整復時に両側房室弁の弁輪形成術を行った犬の1例
  川元 誠; 中村 健介; 森 拓也; 古越 真耶; 大橋 美里; 三木 悠矢; 森下 啓太郎; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 285, 285, (公社)北海道獣医師会, Aug. 2022
  Japanese
• 甲状腺機能低下症の治療により失神の消失を認めた犬の1例
  塩原 希; 中村 健介; 幸野 瑠美; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 285, 285, (公社)北海道獣医師会, Aug. 2022
  Japanese
• 臨床的に間質性肺疾患と診断された犬25頭の回顧的検討
  川上 侑記; 中村 健介; 新坊 弦也; 山根 由久; 大菅 辰幸; 竹内 恭介; 木之下 怜平; 細谷 謙次; 永田 矩之; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 286, 286, (公社)北海道獣医師会, Aug. 2022
  Japanese
• 犬尿中コルチゾール/クレアチニン比の基準値の確立とクッシング症候群の診断への応用
  永田 矩之; 澤村 飛龍; 五日市 大介; 森下 啓太郎; 細谷 謙次; 横山 望; 笹岡 一慶; 佐々木 東; 中村 健介; 池中 良徳; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 286, 286, (公社)北海道獣医師会, Aug. 2022
  Japanese
• 磁気共鳴スペクトロスコピーは犬の起源不明髄膜脳脊髄炎の予後指標となり得るか
  山口 敬央; 笹岡 一慶; 新坊 弦也; 山根 由久; 永田 矩之; 横山 望; 森下 啓太郎; 佐々木 東; 中村 健介; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 287, 287, (公社)北海道獣医師会, Aug. 2022
  Japanese
• 低コレステロール血症は犬の膵外分泌不全を予測できるのか?
  横山 望; 大田 寛; 永田 矩之; 笹岡 一慶; 新坊 弦也; 佐々木 東; 森下 啓太郎; 中村 健介; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 284, 284, (公社)北海道獣医師会, Aug. 2022
  Japanese
• Characterization of mucin gene expression and goblet cell proportion in inflammatory colorectal polyps in miniature dachshunds.
  Noriyuki Nagata; Hiroshi Ohta; Nozomu Yokoyama; Yong Bin Teoh; Noboru Sasaki; Kensuke Nakamura; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 84, 6, 872, 876, 18 Jun. 2022, [Domestic magazines]
  English, Scientific journal, Hyperplastic goblet cells and abundant mucus are significant characteristics of inflammatory colorectal polyps (ICRPs) in miniature dachshunds. In this study, selected mucin gene expressions and goblet cell proportions were evaluated in miniature dachshunds with ICRPs and in healthy dogs. Mucin 2 (MUC2) gene expression was not significantly different among the groups, whereas mucin 5AC (MUC5AC) gene expression was significantly higher in the polypoid lesions than in healthy colonic mucosa. Although the percentage of goblet cells in the upper crypt regions did not significantly differ between the groups, that in the lower crypt regions was significantly decreased in polypoid lesions. In conclusion, increased MUC5AC gene expression and goblet cell proportion changes may be associated with the pathogenesis of ICRPs.
• Urinary corticoid to creatinine ratios using IMMULITE 2000 XPi for diagnosis of canine hypercortisolism.
  Noriyuki Nagata; Hiryu Sawamura; Keitaro Morishita; Kenji Hosoya; Nozomu Yokoyama; Kazuyoshi Sasaoka; Noboru Sasaki; Kensuke Nakamura; Yoshinori Ikenaka; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 84, 7, 954, 959, 27 May 2022, [Domestic magazines]
  English, Scientific journal, The urinary corticoid to creatinine ratio (UCCR) is one of the most commonly used screening tests for canine hypercortisolism (HC). In this study, a reference interval was established for UCCR using IMMULITE 2000 XPi, the latest chemiluminescence enzyme immunoassay. The diagnostic performance of this method for UCCR in canine HC was also evaluated. The median UCCR was 1.06 × 10-5 (range: 0.28-2.49) for 58 healthy dogs, and an upper reference limit of 1.98 × 10-5 (90% confidence interval: 1.76-2.15) was determined. The median UCCR in the 12 dogs with HC (7.38 × 10-5, range 1.86-29.98) was significantly higher than that in the 16 dogs with mimic-HC (1.59 × 10-5, range 0.47-3.42, P<0.001). The area under the curve for UCCR to differentiate HC dogs from mimic-HC dogs was 0.971, with a sensitivity of 91.7% and specificity of 100% when the cut-off value was set at 3.77 × 10-5. The UCCR of 16 paired urine samples collected at home and in hospital showed that the UCCR of samples collected in the hospital was significantly higher than that of samples collected at home (mean difference 3.30 × 10-5, 95% confidence interval: 0.70-5.90, P=0.001). In summary, we established the upper reference limit for UCCR using IMMULITE 2000 XPi in dogs and confirmed that UCCR is a useful diagnostic test for HC in dogs if urine samples are collected at home.
• Incremental predictive value of echocardiographic indices of right ventricular function in the assessment of long-term prognosis in dogs with myxomatous mitral valve disease
  T. Morita; K. Nakamura; T. Osuga; M. Takiguchi
  Journal of Veterinary Cardiology, 39, 51, 62, Feb. 2022
  Scientific journal
• Evaluation of Right Ventricular Function and Dyssynchrony in a Dog Model of Acute Pulmonary Embolism: Diagnostic Utility and Reversibility.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Sei Kawamoto; Shingo Miki; Mitsuyoshi Takiguchi
  Frontiers in veterinary science, 9, 861064, 861064, 2022, [International Magazine]
  English, Scientific journal, Background: The diagnosis of acute pulmonary thromboembolism is challenging in dogs. Little has been reported on changes in echocardiographic indices in dogs with acute pulmonary thromboembolism. The objective of this study was to validate the relationship between echocardiographic indices and right heart catheterization variables in dogs with acute pulmonary embolism and to identify a useful echocardiographic index for diagnosing acute pulmonary embolism. Materials and Methods: Six healthy laboratory beagles were included in the study. Echocardiography and right heart catheterization were performed in a dog model of acute pulmonary embolism produced by microsphere injection. Echocardiographic indices, including the right ventricular (RV) Tei index, RV longitudinal strain, and the dyssynchrony index using speckle tracking echocardiography, transmitral flow, and eccentricity index, were measured. Results: The mean pulmonary arterial pressure increased (22.2 ± 1.2 mmHg) and the blood pressure decreased after microsphere injection. Although the mean pulmonary arterial pressure remained elevated, the blood pressure recovered 2 days after the microsphere injection. Most echocardiographic indices of RV function were significantly impaired following microsphere injection and recovered after 2 days. In contrast, the RV Tei index was significantly impaired after microsphere injection and the impairment persisted after 2 days. Multivariable analysis revealed that the RV Tei index was an independent echocardiographic predictor of pulmonary vascular resistance (β = 0.88, P < 0.001), and transmitral early diastolic wave was an independent predictor of the cardiac index (β = -0.86, P = 0.001). Conclusions: The RV Tei index is a useful echocardiographic index for diagnosing acute pulmonary embolism. Ventricular interdependence may be an important factor causing low cardiac output in dogs with acute pulmonary embolism.
• Safety Assessment of Ultrasound-Assisted Intravesical Chemotherapy in Normal Dogs: A Pilot Study.
  Noboru Sasaki; Yoshinori Ikenaka; Keisuke Aoshima; Teiichiro Aoyagi; Nobuki Kudo; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Frontiers in pharmacology, 13, 837754, 837754, 2022, [International Magazine]
  English, Scientific journal, Intravesical chemotherapy after transurethral resection is a treatment option in patients with non-muscle invasive bladder cancer. The efficacy of intravesical chemotherapy is determined by the cellular uptake of intravesical drugs. Therefore, drug delivery technologies in the urinary bladder are promising tools for enhancing the efficacy of intravesical chemotherapy. Ultrasound-triggered microbubble cavitation may enhance the permeability of the urothelium, and thus may have potential as a drug delivery technology in the urinary bladder. Meanwhile, the enhanced permeability may increase systemic absorption of intravesical drugs, which may increase the adverse effects of the drug. The aim of this preliminary safety study was to assess the systemic absorption of an intravesical drug that was delivered by ultrasound-triggered microbubble cavitation in the urinary bladder of normal dogs. Pirarubicin, a derivative of doxorubicin, and an ultrasound contrast agent (Sonazoid) microbubbles were administered in the urinary bladder. Ultrasound (transmitting frequency 5 MHz; pulse duration 0.44 μsec; pulse repetition frequency 7.7 kHz; peak negative pressure -1.2 MPa) was exposed to the bladder using a diagnostic ultrasound probe (PLT-704SBT). The combination of ultrasound and microbubbles did not increase the plasma concentration of intravesical pirarubicin. In addition, hematoxylin and eosin staining showed that the combination of ultrasound and microbubble did not cause observable damages to the urothelium. Tissue pirarubicin concentration in the sonicated region was higher than that of the non-sonicated region in two of three dogs. The results of this pilot study demonstrate the safety of the combination of intravesical pirarubicin and ultrasound-triggered microbubble cavitation, that is, ultrasound-assisted intravesical chemotherapy.
• Effects of intravenous administration of pimobendan on hemodynamic indices and indices of left atrial longitudinal strain derived from speckle-tracking echocardiography in healthy dogs.
  Shingo Miki; Kensuke Nakamura; Tomoya Morita; Tatsuyuki Osuga; Sei Kawamoto; Noboru Sasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 82, 10, 795, 801, Oct. 2021, [International Magazine]
  English, Scientific journal, OBJECTIVE: To determine the effects of IV administration of pimobendan on hemodynamic indices and indices of left atrial (LA) longitudinal strain by speckle-tracking echocardiography in healthy dogs. ANIMALS: 6 healthy Beagles. PROCEDURES: After the dogs were anesthetized, the right heart was catheterized and 2-D conventional and speckle-tracking echocardiography were performed before and after IV administration of 0.15 mg of pimobendan/kg. Speckle-tracking echocardiography was performed to assess the 3 LA phasic functions through LA deformation (longitudinal strain and strain rate) and volumetric analyses. RESULTS: Pimobendan significantly increased stroke volume and cardiac output and decreased systemic vascular resistance. Pimobendan significantly improved left ventricular function assessed by the Tei index and LA booster pump function assessed by LA longitudinal strain and change in fractional volume during atrial systole. Indices of LA reservoir and conduit function were unchanged. CONCLUSIONS AND CLINICAL RELEVANCE: In healthy dogs, IV administration of pimobendan improved the indices of LA booster pump function but had no effect on the indices of LA reservoir and conduit functions. Further studies are needed to validate whether these results can be extrapolated to dogs with myxomatous mitral valve disease.
• Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method.
  Noboru Sasaki; Thandar Oo; Yoshikazu Yasuda; Takahiro Ichise; Noriyuki Nagata; Nozomu Yokoyama; Kazuyoshi Sasaoka; Keitaro Morishita; Shouta Mm Nakayama; Mayumi Ishizuka; Kensuke Nakamura; Mitsuyoshi Takiguchi; Yoshinori Ikenaka
  The Journal of veterinary medical science, 83, 11, 1634, 1642, 17 Sep. 2021, [Domestic magazines]
  English, Scientific journal, We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2-16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.
• イヌの肝細胞腫瘍におけるDNAメチル化の網羅的解析
  淺利 友; 山崎 淳平; Oo Thander; 細谷 謙次; 金 尚昊; 木之下 怜平; 竹内 恭介; 賀川 由美子; 佐々木 東; 中村 健介; 滝口 満喜
  日本獣医学会学術集会講演要旨集, 164回, [HSO, 30], (公社)日本獣医学会, Sep. 2021
  Japanese
• 非再生性免疫介在性貧血の犬における脾臓摘出術前後の血清中蛋白の網羅的解析
  菅原 芽伊; 山崎 淳平; 森下 啓太郎; 金 尚昊; 細谷 謙次; 佐々木 東; 中村 健介; 滝口 満喜
  日本獣医学会学術集会講演要旨集, 164回, [HSO, 60], (公社)日本獣医学会, Sep. 2021
  Japanese
• Cushing症候群と肝細胞癌を合併した犬におけるステロイド値の包括的解析(Comprehensive analysis of steroid profile in dogs with Cushing' syndrome and hepatocellular carcinoma)
  Oo Thandar; 佐々木 東; 池中 良徳; 永田 矩之; 一瀬 貴大; Bin Teoh Yong; 川元 誠; 小島 一輝; 菅原 芽伊; 中山 翔太; 石塚 真由美; 中村 健介; 滝口 満喜
  日本獣医学会学術集会講演要旨集, 164回, [HSO, 18], (公社)日本獣医学会, Sep. 2021
  English
• Cardiovascular effects of dose escalating of norepinephrine in healthy dogs anesthetized with isoflurane.
  Kazuki Kojima; Tomohito Ishizuka; Noboru Sasaki; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Veterinary anaesthesia and analgesia, 48, 5, 654, 662, Sep. 2021, [International Magazine]
  English, Scientific journal, OBJECTIVE: To evaluate the systemic cardiovascular effects of dose escalating administration of norepinephrine in healthy dogs anesthetized with isoflurane. STUDY DESIGN: Experimental study. ANIMALS: A total of six adult laboratory Beagle dogs, 10.5 (9.2-12.0) kg [median (range)]. METHODS: Each dog was anesthetized with isoflurane at an end-tidal concentration of 1.7%, mechanically ventilated and administered a continuous rate infusion of rocuronium (0.5 mg kg-1 hour-1). Each dog was administered incremental dose rates of norepinephrine (0.05, 0.125, 0.25, 0.5, 1.0 and 2.0 μg kg-1 minute-1), and each dose was infused for 15 minutes. Cardiovascular variables were recorded before administration and at the end of each infusion period. RESULTS: Norepinephrine infusion increased mean arterial pressure (MAP), cardiac output (CO) and oxygen delivery in a dose-dependent manner. Systemic vascular resistance did not significantly change during the experiment. Stroke volume increased at the lower dose rates and heart rate increased at the higher dose rates. Oxygen consumption and lactate concentrations did not significantly change during infusions. CONCLUSIONS: In dogs anesthetized with isoflurane, norepinephrine increased MAP by increasing the CO. CO increased with a change in stroke volume at lower dose rates of norepinephrine. At higher dosage, heart rate also contributed to an increase in CO. Norepinephrine did not cause excessive vasoconstriction that interfered with the CO during this study. CLINICAL RELEVANCE: Norepinephrine can be useful for treating hypotension in dogs anesthetized with isoflurane.
• Pancreatic enzyme activity in the bile of healthy cats and its association with biliary morphology.
  Shinsuke Fujimoto; Shidow Torisu; Yasuyuki Kaneko; Shinya Mizutani; Shushi Yamamoto; Kiyokazu Naganobu; Kensuke Nakamura
  Journal of veterinary internal medicine, 35, 4, 1780, 1788, 12 Jun. 2021, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, BACKGROUND: In human medicine, congenital maljunction of the common bile duct (CBD) and main pancreatic duct (MPD), or pancreatobiliary maljunction (PBM), is a known cause of cholecystitis. OBJECTIVE: Pancreatic enzyme activity in the bile (a diagnostic marker for PBM) of healthy cats was measured to determine normal values and evaluate its relationship with biliary morphology. ANIMALS: Fifty-two healthy cats. METHODS: Cross-sectional study of the biliary tracts of healthy cats during laparoscopic ovariohysterectomy and measurement of pancreatic enzyme activity in bile. The cats were divided into groups A and B based on the ratio of the diameter of the cystic duct (CD) to the CBD. The normal ratio was 3.4. Pancreatic enzyme activity in bile was compared between the groups. RESULTS: The CBDs were straight in all cases, whereas the CDs were variably tortuous or dilated. Amylase activity in the bile (median, <100 U/L; range, <100-591 U/L) was lower than in serum in all cases, and group B, which had a CD/CBD ratio >3.4, had significantly higher amylase activity (median, 109 U/L; range, <100-591 U/L) in the bile than did group A (median, <100 U/L; range, <100-238 U/L), which had a CD/CBD ratio <3.4 (P = .0009). CONCLUSIONS AND CLINICAL IMPORTANCE: The results suggest that a dilated CD is associated with reflux of pancreatic juice. In the future, it will be necessary to examine the clinical usefulness of these findings by measuring pancreatic enzyme activity in the bile of cats with cholangitis.
• Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method.
  Noboru Sasaki; Yoshinori Ikenaka; Yumiko Inoue; Takahiro Ichise; Noriyuki Nagata; Mayumi Ishizuka; Syouta Mm Nakayama; Kensuke Nakamura; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 83, 4, 648, 655, 09 Apr. 2021, [Domestic magazines]
  English, Scientific journal, Measurement of urinary metanephrines in spot samples is used for the diagnosis of canine pheochromocytoma (PC). We describe a simple analytical method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for measuring free metanephrine (MN) and normetanephrine (NMN) in spot urine samples. Using the developed method, we evaluated the stability of urinary free-MN and free-NMN at various storing conditions. In addition, we assessed the feasibility of urinary free-MN and -NMN measurement for diagnosing PC. Urine samples were mixed with stable isotope internal standards and thereafter purified by ultrafiltration. The purified samples were analyzed by LC-MS/MS in the multiple reaction monitoring mode after separation on a multimode octa decyl silyl column. The coefficient of variation of free-MN and -NMN measurement was 7.6% and 5.5%, respectively. The linearity range was 0.5-10 µg/l for both analytes. Degradation was less than 10% for both analytes under any of the storage conditions. The median free-NMN ratio to creatinine of 9 PC dogs (595, range 144-47,961) was significantly higher (P<0.05) than that of 13 dogs with hypercortisolism (125, range 52-224) or 15 healthy dogs (85, range 50-117). The developed method is simple and may not require acidification of spot urine. The results of this preliminary retrospective study suggest that the measurement of urinary free metanephrines is a promising tool for diagnosing canine PC.
• 超音波で生体の壁を超える 超音波と気泡を併用する膀胱内注入療法の犬における安全性検討
  佐々木 東; 青柳 貞一郎; 池中 良徳; 工藤 信樹; 中村 健介; 滝口 満喜
  超音波医学, 48, Suppl., S195, S195, (公社)日本超音波医学会, Apr. 2021
  Japanese
• Repeatability, reproducibility, and reference intervals for indices of right atrial longitudinal strain derived from speckle-tracking echocardiography in healthy dogs.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Mitsuyoshi Takiguchi
  American journal of veterinary research, 82, 4, 274, 279, Apr. 2021, [International Magazine]
  English, Scientific journal, OBJECTIVE: To determine repeatability, reproducibility, and reference intervals of indices of right atrial longitudinal strain (RALS) derived from speckle-tracking echocardiography (STE) in dogs without heart disease. ANIMALS: 110 client-owned dogs and 10 laboratory Beagles. PROCEDURES: To determine intraobserver within-day (repeatability) and interobserver (reproducibility) coefficients of variation, RALS during ventricular systole (ϵS), ventricular early diastole (ϵE), and atrial systole (ϵA), as derived with STE, were obtained by 2 investigators for 5 randomly selected client-owned dogs and analyzed by linear regression. Reference intervals were estimated from the results of all dogs. Correlations between RALS indices (ϵS, ϵE, and ϵA) and sex, age, body weight, heart rate, and blood pressure were determined. RESULTS: RALS derived from STE showed good intraobserver within-day repeatability and interobserver reproducibility, with coefficients of variation of < 20%. Both ϵS and ϵE were significantly negatively correlated with age, but ϵA was not correlated with age. Indices were not correlated with sex, body weight, or blood pressure. CONCLUSIONS AND CLINICAL RELEVANCE: RALS indices derived from STE were repeatable and reproducible and were affected by the age of dogs without heart disease. Age should be considered in the interpretation of RALS indices in clinical settings. Further studies are needed to apply RALS indices for assessing dogs with heart disease.
• Ultrasonographic evaluation of the caudal vena cava in dogs with right-sided heart disease.
  T Fujioka; K Nakamura; T Minamoto; N Tsuzuki; J Yamaguchi; Y Hidaka
  Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology, 34, 80, 92, Apr. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, INTRODUCTION/OBJECTIVES: In humans with impaired right-sided cardiac function, the caudal vena cava (CVC) diameter serves as a marker of venous congestion. This study aimed to investigate whether ultrasonographic CVC variables could identify the presence of right-sided congestive heart failure (R-CHF) in dogs with right-sided heart disease (RHD). ANIMALS: Fifty client-owned control dogs and 67 dogs with RHD were enrolled. The dogs with RHD were subdivided into the non-R-CHF (n = 43) and R-CHF (n = 24) groups. MATERIALS AND METHODS: We measured and compared the ultrasonographic CVC variables and echocardiographic variables among the groups. Receiver operating characteristic (ROC) curve analysis was performed to calculate the sensitivity and specificity of the variables at optimal cutoff values. RESULTS: We obtained the highest accuracies of the ratio of the shortest diameter (SD) of the minimal CVC area to the aorta diameter (Ao) during inspiration [SD(min)/Ao] and of the ratio of SD(min) to the longest diameter of the minimal CVC area during inspiration [LD(min),SD/LD(min)], with high sensitivities, specificities, and an area under the ROC curve greater than 0.925. CONCLUSIONS: In addition to the echocardiographic assessment of right-sided cardiac function, the CVC variables in this study, especially SD(min)/Ao and SD/LD(min), would be useful diagnostic indices for identifying R-CHF in dogs with RHD.
• Acute effects of intravenous pimobendan administration in dog models of chronic precapillary pulmonary hypertension.
  T Morita; K Nakamura; T Osuga; S Kawamoto; S Miki; K Sasaoka; M Takiguchi
  Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology, 32, 16, 27, Dec. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, INTRODUCTION: Pulmonary hypertension (PH) is a progressive and potentially life-threatening disease. Several drugs are used for the treatment of dogs with precapillary PH. Pimobendan is an inotropic drug with phosphodiesterase 3 inhibitory and calcium-sensitizing effects. Pimobendan administration improved right ventricular (RV) function and lowered pulmonary arterial pressure in some human patients with precapillary PH. However, the efficacy of pimobendan in dogs with precapillary PH is unknown. ANIMALS, MATERIAL, AND METHODS: An implantable port device was percutaneously placed in the cranial vena cava of five laboratory beagles. Chronic embolic precapillary PH was induced via the repeated injection of microspheres every 1-2 days. Microsphere injection was continued until systolic pulmonary arterial pressure reached 50 mmHg. Right heart catheterization and echocardiography were performed at baseline and after injections of placebo and pimobendan (0.15 mg/kg). RESULTS: Repeated injections of microspheres caused an increase in pulmonary vascular resistance, a decrease in stroke volume, RV dilation, left ventricular (LV) and RV dysfunction, and RV dyssynchrony as assessed using echocardiography. Compared with placebo, pimobendan improved LV and RV function based on the LV Tei index from 0.48 to 0.38 (p=0.002) and the RV Tei index from 0.76 to 0.61 (p=0.008), as well as the stroke volume index from 29.4 to 36.7 ml/m2 (p=0.012), respectively. CONCLUSIONS: In dog models of chronic PH, intravenous pimobendan effectively improved RV and LV function and increased stroke volume. However, pimobendan administration did not decrease pulmonary arterial pressure or produce hypotension.
• Podoplanin expression on endothelial cells promotes superficial erosive injury and thrombus formation in rat carotid artery: Implications for plaque erosion.
  Eiji Furukoji; Atsushi Yamashita; Kensuke Nakamura; Toshinori Hirai; Yujiro Asada
  Thrombosis research, 183, 76, 79, Nov. 2019, [Peer-reviewed], [International Magazine]
  English
• Change in right ventricular function in an American cocker spaniel with acute pulmonary thromboembolism.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Kiwamu Hanazono; Keitaro Morishita; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 9, 1259, 1265, 03 Sep. 2019, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, A 12-year-old neutered female American cocker spaniel weighing 9.9 kg was presented for evaluation with a 2-day history of dyspnea and anorexia. Echocardiography revealed severe pulmonary hypertension (estimated systolic pulmonary arterial pressure, 93.4 mmHg) with right heart enlargement, pulmonary arterial dilation, and right ventricular dysfunction. The dilation of left heart and congenital cardiac shunt were not observed. Pulmonary thromboembolism (PTE) was confirmed by computed tomographic angiography. After treatment with antiplatelet and anticoagulant, the clinical sign and the echocardiographic abnormality of right heart were improved. These echocardiographic findings are not specific for PTE, but it can be useful as a rule-in test for PTE when other causes of pulmonary hypertension are excluded and a monitor of therapeutic efficacy.
• Findings of contrast-enhanced ultrasonography with Sonazoid for cholangiocellular adenoma in three dogs.
  Masahiro Tamura; Kensuke Nakamura; Tatsuyuki Osuga; Genya Shimbo; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 8, 1104, 1108, 09 Aug. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Contrast-enhanced ultrasonography (CEUS) is useful to distinguish benign and malignant focal liver lesions in dogs. Cholangiocellular adenoma is an extremely rare benign tumor in dogs and has not been examined using CEUS with Sonazoid. The aim of this study was to describe findings of CEUS with Sonazoid in three dogs with cholangiocellular adenoma. All three dogs showed contrast defects in the Kupffer phase and these findings mimicked malignant neoplasia during the Kupffer phase. Moreover, all dogs showed early washout and hypoechoic lesions relative to the surrounding normal liver parenchyma in the portal phase. To our knowledge, this is the first study to report that CEUS findings of cholangiocellular adenoma with Sonazoid mimicked malignancy in three dogs.
• Effect of ultra low fat diet on regulatory T cells of dogs with intestinal limphangiectasia secondary to chronic enteropathy: A pilot study
  Khoirun Nisa; Hiroshi Ohta; Nozomu Yokoyama; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Rommaneeya Leela-Arporn; Tomoya Morita; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Yumiko Kagawa; Mitsuyoshi Takiguchi
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 67, 3, 221, 227, Aug. 2019, [Peer-reviewed]
  English, Scientific journal
• Effect of acute volume loading on left atrial strain values derived from two-dimensional speckle tracking echocardiography in dogs.
  Angkhana Dermlim; Tatsuyuki Osuga; Kensuke Nakamura; Tomoya Morita; Khoirun Nisa; Kazuyoshi Sasaoka; Rommaneeya Leela-Arporn; Noriyuki Nagata; Masahiro Tamura; Noboru Sasaki; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 7, 949, 957, 11 Jul. 2019, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, The purpose of this study was to evaluate the cardiac acute volume loading effect on left atrial (LA) strain and strain rate (SR) parameters derived from two-dimensional speckle tracking echocardiography (2D-STE) in healthy dogs. Six healthy beagles were anesthetized and subjected to increase cardiac preload by intravenous infusion with lactated Ringer solution at 150 ml/kg/hr for 90 min. A Swan-Ganz catheter was placed to directly measure the mean pulmonary capillary wedge pressure (PCWP). Echocardiography was performed before (baseline) and at 15, 30, 45, 60, 75, and 90 min after acute volume loading began. Apical 4-chamber images focused on the LA were digitally recorded for later strain and SR analysis via 2D-STE. Acute volume loading significantly increased from baseline during LA strain and SR as assessed by the speckle tracking-based technique during reservoir and conduit function at 15 to 90 min after volume load began, and strain indices representing booster pump function were enhanced at 45 to 90 min. In addition, acute volume loading resulted in a significantly greater PCWP after fluid infusion. On multiple regression analysis, quadratic regression analysis was a better fit for the relationship between PCWP and all LA functional indices. Our findings indicated that LA function analyzed by strain and SR was enhanced during cardiac acute volume loading in healthy dogs. The change in strain and SR during acute volume loading should be interpreted with caution during the diagnosis of heart diseases related to volume overload.
• What Is Your Diagnosis?
  Satoshi Takagi; Kensuke Nakamura; Ryo Takeuchi; Kiwamu Hanazono
  Journal of the American Veterinary Medical Association, 255, 1, 51, 53, 01 Jul. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• The Repeatability and Left Atrial Strain Analysis Obtained via Speckle Tracking Echocardiography in healthy Dogs
  A. Dermlim; K. Nakamura; T. Morita; T. Osuga; K. Nisa; K. Sasaoka; R. Leela-arporn; N. Sasaki; H. Ohta; M. Takiguchi
  Journal of Veterinary Cardiology, 23, 69, 80, Elsevier BV, Jun. 2019
  Scientific journal
• Right ventricular function and dyssynchrony measured by echocardiography in dogs with precapillary pulmonary hypertension
  T. Morita; K. Nakamura; T. Osuga; K. Morishita; N. Sasaki; H. Ohta; M. Takiguchi
  Journal of Veterinary Cardiology, 23, 1, 14, Elsevier BV, Jun. 2019
  Scientific journal
• Epidemiology of massive hepatocellular carcinoma in dogs: A 4-year retrospective study.
  Leela-Arporn R; Ohta H; Nagata N; Sasaoka K; Tamura M; Dermlim A; Nisa K; Morishita K; Sasaki N; Nakamura K; Takagi S; Hosoya K; Takiguchi M
  Veterinary journal (London, England : 1997), 248, 74, 78, Elsevier BV, Jun. 2019, [Peer-reviewed]
  Scientific journal
• Predictive factors of malignancy in dogs with focal liver lesions using clinical data and ultrasonographic features.
  Rommaneeya Leela-Arporn; Hiroshi Ohta; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Khoirun Nisa; Keitaro Morishita; Noboru Sasaki; Kensuke Nakamura; Satoshi Takagi; Kenji Hosoya; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 5, 723, 729, 31 May 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, A definitive diagnosis of focal liver lesions (FLLs) requires invasive procedures for histopathologic examination. Thus, a simpler noninvasive diagnostic method, such as conventional ultrasonography combined with clinical data, is needed for the prediction of liver malignancy. The objective of this study was to examine the diagnostic accuracy of clinical data and ultrasonographic (US) features to differentiate benign and malignant liver lesions. Medical records and US images from dogs with FLLs that underwent abdominal US and histopathologic examinations following surgery or liver biopsy were retrospectively reviewed. Clinical data, including signalment, clinical signs and laboratory findings, and the US features of liver lesions that could act as predictive factors were assessed using univariate and multivariate analyses to evaluate the associations between predictive factors and liver malignancy. Based on the histopathologic results, 55 dogs with malignant lesions and 28 dogs with benign lesions were included in the study. The results of univariate analysis showed that several US features and platelet count were significantly associated with liver malignancy. Multivariate analysis revealed that the platelet count (thrombocytosis; odds ratio [OR]: 4.13, 95% confidence intervals [CI]: 1.81-9.41), lesion size (4.1 cm or greater; OR: 23.83, 95% CI: 3.74-151.95) and echotexture of FLLs (heterogenous; OR: 8.44; 95% CI: 1.37-51.91) were independent predictors for differentiating benign and malignant liver lesions, suggesting that a combination of clinical data and US findings of FLLs could predict liver malignancy in dogs.
• Epidemiological study of indirect blood pressure measured using oscillometry in clinically healthy cats at initial evaluation.
  Yasutomo Hori; Yasuhiro Heishima; Yohei Yamashita; Masayuki Iguchi; Kensuke Nakamura; Noriko Isayama; Hideyuki Onodera; Akihito Kitade; Nobuyuki Kanno; Atsushi Hirakawa; Michio Katagi; Toshiki Ibaragi; Shigeki Yamano; Yoshitaka Aramaki; Tamotsu Sawada
  The Journal of veterinary medical science, 81, 4, 513, 516, 16 Apr. 2019, [Peer-reviewed], [Domestic magazines]
  English, We investigated the clinical characteristics of healthy cats in accordance with the target organ damage (TOD) risk category, on the basis of systolic blood pressure (SBP). This prospective multi-center study included 137 healthy cats. Indirect blood pressure was measured using an oscillometric technique. The median SBP in all cats was 147 mmHg (interquartile range: 134-158). On the basis of the TOD risk category, 57.7, 19.7, 21.9, and 0.7% of the cats were classified into categories I-IV, respectively. Age, sex, and body weight did not affect the SBP. This study provides basic information on the distribution of TOD risk categories in clinically healthy cats.
• Evaluation of duodenal perfusion by contrast-enhanced ultrasonography in dogs with chronic inflammatory enteropathy and intestinal lymphoma.
  Nisa K; Lim SY; Shinohara M; Osuga T; Yokoyama N; Tamura M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 33, 2, 559, 568, Mar. 2019, [Peer-reviewed]
  English, Scientific journal
• Evaluation of duodenal perfusion by contrast-enhanced ultrasonography in dogs with chronic inflammatory enteropathy and intestinal lymphoma.
  Khoirun Nisa; Sue Yee Lim; Masayoshi Shinohara; Tatsuyuki Osuga; Nozomu Yokoyama; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Rommaneeya Leela-Arporn; Tomoya Morita; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 33, 2, 559, 568, Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Contrast-enhanced ultrasonography (CEUS) can be used to evaluate intestinal perfusion in healthy dogs. It is helpful for diagnosing and monitoring inflammatory bowel disease in humans and could be useful for dogs with chronic intestinal diseases. OBJECTIVES: To examine duodenal perfusion in dogs with chronic inflammatory enteropathy (CIE) and intestinal lymphoma. ANIMALS: Client-owned dogs with CIE (n = 26) or intestinal lymphoma (n = 7) and dogs with gastrointestinal signs but histopathologically normal duodenum (controls, n = 14). METHODS: In this cross-sectional study, dogs with CIE were classified into remission (n = 16) and symptomatic (n = 10) groups based on clinical scores determined at the time of CEUS. The duodenum was scanned after IV injection of Sonazoid® (0.01 mL/kg). CEUS-derived perfusion parameters, including time-to-peak, peak intensity (PI), area under the curve (AUC), and wash-in and wash-out rates were evaluated. RESULTS: The PI was significantly higher in the symptomatic CIE group (median (range); 105.4 (89.3-128.8) MPV) than in the control group (89.9 (68.5-112.2) MPV). The AUC was significantly higher in the symptomatic CIE group (4847.9 (3824.3-8462.8) MPV.sec) than in the control (3448.9 (1559.5-4736.9) MPV.sec) and remission CIE (3862.3 (2094.5-6899.0) MPV.sec) groups. The PI and clinical score were positively correlated in the CIE group. No significant differences in perfusion parameters were detected between the lymphoma and CIE groups or the lymphoma and control groups. CONCLUSIONS AND CLINICAL IMPORTANCE: The PI and AUC can detect duodenal inflammation and hence are potentially useful for excluding a diagnosis of CIE.
• Corrigendum: Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats.
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in veterinary science, 6, 2, 2, 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, [This corrects the article DOI: 10.3389/fvets.2018.00289.].
• Effect of acute volume overload on echocardiographic indices of right ventricular function and dyssynchrony assessed by use of speckle tracking echocardiography in healthy dogs.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Nozomu Yokoyama; Keitaro Morishita; Noboru Sasaki; Hiroshi Ohta; Mitsuyoshi Takiguchi
  American journal of veterinary research, 80, 1, 51, 60, Jan. 2019, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVE To determine the relationship between acute volume overload and echocardiographic indices of right ventricular (RV) function and dyssynchrony in healthy dogs. ANIMALS 7 healthy Beagles. PROCEDURES Right heart catheterization and echocardiography were performed in 7 healthy anesthetized Beagles at baseline and after induction of volume overload. Volume overload was induced by IV infusion of lactated Ringer solution (150 mL/kg/h for 90 minutes). Echocardiographic indices of RV function, including peak velocity of systolic tricuspid annular motion, tricuspid annulus plane systolic excursion, fractional area change, RV Tei index, RV longitudinal strain (RVLS), and systolic RV longitudinal strain rate (RVLSR), were obtained by use of speckle tracking echocardiography (STE). In addition, SD of the systolic shortening time of the right ventricle for the 6 segments (RV-SD6) was determined with STE. RESULTS Volume overload significantly increased the RV end-diastolic pressure, compared with the baseline value. Echocardiographic indices of RV function, except for septal RVLSR, were significantly enhanced by volume overload. In contrast, RV-SD6 did not change with volume overload. Although echocardiographic indices of RV function, except for septal RVLSR, were correlated with RV end-diastolic pressure, RV-SD6 was not correlated. CONCLUSIONS AND CLINICAL RELEVANCE Echocardiographic indices of RV function, including RVLS and RVLSR, were affected by acute short-term volume overload. Therefore, results for assessment of RV function by use of STE in dogs with clinical conditions associated with right-sided chronic volume overload, such as tricuspid and pulmonic regurgitation, should be interpreted with caution.
• Effects of immunosuppressive prednisolone therapy on pancreatic tissue and concentration of canine pancreatic lipase immunoreactivity in healthy dogs.
  Hiroshi Ohta; Kazuki Kojima; Nozomu Yokoyama; Noboru Sasaki; Yumiko Kagawa; Kiwamu Hanazono; Tomohito Ishizuka; Keitaro Morishita; Kensuke Nakamura; Satoshi Takagi; Mitsuyoshi Takiguchi
  Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 82, 4, 278, 286, Oct. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The objective of this study was to examine the effects of immunosuppressive prednisolone therapy on pancreatic tissue and the concentration of serum canine pancreatic lipase immunoreactivity (cPLI) in healthy dogs. Six healthy beagle dogs were subcutaneously administered an immunosuppressive dose of prednisolone [4 mg/kg body weight (BW)] once daily for either 2 or 3 weeks. Serum cPLI concentration was measured before and after treatment. Ultrasonographic examination of the pancreas and laparoscopic biopsy and histopathological examination of the right pancreatic lobe and the liver were also conducted before and after treatment. The expression of pancreatic lipase messenger ribonucleic acid (mRNA) in the pancreas and liver was examined by polymerase chain reaction (PCR). Although the serum cPLI concentration was significantly higher on day 14 and on the day of the second laparoscopy than before treatment, it was classified as normal (≤ 200 μg/L) in 5 dogs and as abnormal (≥ 400 μg/L) in only 1 dog. None of the 6 dogs showed clinical signs of pancreatitis during the study period. After treatment, ultrasonographic examination of the pancreas showed no changes except for a hypoechoic pancreas in 1 dog. Histopathological examination of the right pancreatic lobe in all dogs showed no evidence of pancreatitis after treatment. Pancreatic lipase mRNA expression was detected in the pancreas, but not in the liver, before and after treatment. The administration of 4 mg/kg BW per day of prednisolone for 2 or 3 weeks increased the serum cPLI concentration without clinical signs of pancreatitis, although an abnormal cPLI concentration (≥ 400 μg/L) was observed in only 1 dog. No ultrasonographic or histological evidence of pancreatitis was observed in any of the dogs.
• ECG of the Month.
  Tatsuyuki Osuga; Kensuke Nakamura; Tomoya Morita; Kiyomasa Watanabe; Mitsuyoshi Takiguchi
  Journal of the American Veterinary Medical Association, 253, 4, 417, 420, 15 Aug. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
  English
• Effects of the angiotensin-converting enzyme inhibitor alacepril in dogs with mitral valve disease.
  Yasutomo Hori; Kensuke Nakamura; Nobuyuki Kanno; Makoto Hitomi; Yohei Yamashita; Satoshi Hosaka; Noriko Isayama; Takahiro Mimura
  The Journal of veterinary medical science, 80, 8, 1212, 1218, 10 Aug. 2018, [Peer-reviewed], [Domestic magazines]
  English, Alacepril is a relatively novel angiotensin-converting enzyme inhibitor; however, the safety, tolerance, and efficacy of alacepril in terms of cough suppression in dogs with mitral valve disease (MVD) remain unknown. The aim of this study was to investigate the safety, tolerance, and cough suppression efficacy of alacepril in dogs with MVD. This was a multi-center, prospective study. Forty-two dogs with echocardiographic or radiographic evidence of cardiac enlargement in addition to cough were enrolled. Dogs were treated with alacepril (1.0-3.0 mg/kg/day) for at least 4 weeks. One dog (2.4%) developed complications, including appetite loss, lethargy, and vomiting. Thirty-six dogs were re-evaluated after 4 weeks of treatment. Cough resolved or improved in 20 dogs (55.6%) after treatment. Based on the efficacy of alacepril, the dogs were divided into an effective group (n=20) and an ineffective group (n=16). After treatment, the left ventricular end-diastolic internal diameter corrected for body weight was significantly increased from baseline in the ineffective group but was significantly decreased in the effective group. Univariate binomial logistic regression analyses showed that high atrial natriuretic peptide level, N-terminal pro-B-type natriuretic peptide level, and E wave velocity at baseline were significantly correlated with alacepril inefficacy. Alacepril as treatment for MVD is well tolerated in most dogs, and different conditions of cardiac loading may influence the effect of the drug. Alacepril is expected to improve the quality of life of dogs with early stage MVD.
• Pulmonary hypertension due to unclassified interstitial lung disease in a Pembroke Welsh corgi.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Atsushi Kobayashi; Osamu Ichii; Akira Yabuki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 80, 6, 939, 944, 29 Jun. 2018, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, A 12 year-old intact male Pembroke Welsh corgi weighing 10.8 kg was presented for evaluation of a 3-month history of dyspnea, and a 1-week history of exercise intolerance and anorexia. Severe hypoxemia (PaO2 56 mmHg), diffuse lung alveolar infiltration, and severe pulmonary hypertension (PH) (tricuspid regurgitation pressure gradient was 81 mmHg) were identified. A tentative diagnosis of severe PH due to lung disease or pulmonary thromboembolism was made and treated intensively. After 5 days of hospitalization, the dog died despite oxygen supplementation and anticoagulant therapy. This dog was diagnosed as unclassified interstitial lung disease based on histopathological findings.
• Diagnostic utility of cardiac troponin I in cats with hypertrophic cardiomyopathy.
  Yasutomo Hori; Masayuki Iguchi; Yasuhiro Heishima; Yohei Yamashita; Kensuke Nakamura; Atsushi Hirakawa; Akihito Kitade; Toshiki Ibaragi; Michio Katagi; Tamotsu Sawada; Masashi Yuki; Nobuyuki Kanno; Haruki Inaba; Noriko Isayama; Hideyuki Onodera; Naoki Iwasa; Mikio Kino; Mikihiro Narukawa; Syuhei Uchida
  Journal of veterinary internal medicine, 32, 3, 922, 929, May 2018, [Peer-reviewed], [International Magazine]
  English, BACKGROUND: Cardiac troponin I (cTnI) is useful for assessing hypertrophic cardiomyopathy (HCM) in cats. OBJECTIVE: To measure plasma cTnI concentrations in healthy cats and evaluate the clinical utility of cTnI in determining the severity of HCM. ANIMALS: Clinically healthy cats (n = 88) and cats with HCM (n = 93). METHODS: Multicenter prospective study. Cats with HCM, including hypertrophic obstructive cardiomyopathy at various stages, were diagnosed using echocardiography. Plasma cTnI concentrations were analyzed by a commercial laboratory. Receiver-operating characteristic curve analysis was used to evaluate the accuracy of plasma cTnI concentrations to detect HCM. RESULTS: The median cTnI concentration was 0.027 ng/mL (interquartile range, 0.012-0.048 ng/mL) in healthy cats. Concentrations were significantly higher in diseased cats than in healthy controls, and concentrations were significantly higher in cats with heart failure than in asymptomatic cats. A plasma cTnI concentration of 0.163 ng/mL had a sensitivity of 62.0% and specificity of 100% when used to distinguish normal cats from asymptomatic HCM cats without left atrial dilatation. A cutoff of 0.234 ng/mL had high sensitivity (95.0%) and specificity (77.8%) for assessing heart failure. The areas under the receiver-operating characteristic curves were 0.85 and 0.93, respectively. CONCLUSIONS AND CLINICAL IMPORTANCE: Increased cTnI concentrations reflect the severity of HCM. If other causes of cardiac injury are ruled out, plasma cTnI concentration may be useful for predicting the severity of HCM in cats.
• The effect of sedation with a combination of butorphanol and midazolam on quantitative contrast-enhanced ultrasonography of duodenum in healthy dogs.
  Khoirun Nisa; Sue Yee Lim; Tatsuyuki Osuga; Nozomu Yokoyama; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Rommaneeya Leela-Arporn; Tomoya Morita; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 80, 3, 453, 459, 24 Mar. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Quantitative contrast-enhanced ultrasonography (CEUS) enables non-invasive and objective evaluation of intestinal perfusion by quantifying the intensity of enhancement on the intestine after microbubble contrast administration. During CEUS scanning, sedation is sometimes necessary to maintain animal cooperation. Nevertheless, the effect of sedative administration on the canine intestinal CEUS is unknown. This study aimed to investigate the effect of sedation with a combination of butorphanol and midazolam on the duodenal CEUS-derived perfusion parameters of healthy dogs. For this purpose, duodenum was imaged following contrast administration (Sonazoid®, 0.01 ml/kg) in six healthy beagles before and after intravenous injection of a combination of butorphanol (0.2 mg/kg) and midazolam (0.1 mg/kg). Furthermore, hemodynamic parameters including blood pressure and heart rate were recorded during the procedure. Five CEUS derived perfusion parameters including time-to-peak (TTP), peak intensity (PI), area under the curve (AUC), wash-in and wash-out rates (WiR and WoR, respectively) before and after sedation were statistically compared. The result showed that no significant change was detected in any of perfusion parameters. Systolic and mean arterial pressures significantly reduced after sedative administration, but diastolic arterial pressure and heart rate did not significantly change. Moreover, no significant partial correlation was observed between perfusion parameters and hemodynamic parameters. Thus, we concluded that the combination did not cause significant influence in duodenal CEUS perfusion parameters and could be a good option for sedation prior to duodenal CEUS in debilitated dogs.
• Relationship between indirect blood pressure and various stages of chronic kidney disease in cats.
  Yasutomo Hori; Yasuhiro Heishima; Yohei Yamashita; Noriko Isayama; Nobuyuki Kanno; Kensuke Nakamura; Masayuki Iguchi; Toshiki Ibaragi; Hideyuki Onodera; Yoshitaka Aramaki; Atsushi Hirakawa; Shigeki Yamano; Michio Katagi; Akihito Kitade; Tamotsu Sawada
  The Journal of veterinary medical science, 80, 3, 447, 452, 24 Mar. 2018, [Peer-reviewed], [Domestic magazines]
  English, Chronic kidney disease (CKD) is a common cause of secondary systemic hypertension in cats. We investigated the relationship between indirect blood pressure and the prevalence of systemic hypertension in various CKD stages in cats. Client-owned cats (24 control cats and 77 cats with CKD) were included. Biochemical examinations of plasma were conducted by a commercial laboratory. Diseased cats were divided into two groups based on the International Renal Interest Society (IRIS) guidelines (II and III-IV). Indirect blood pressure was measured using an oscillometric technique. Severe hypertension was diagnosed if systolic blood pressure (SBP) was ≥180 mmHg. Indirect blood pressures were significantly higher in IRIS stage III-IV than in the control cats. Of 77 cats with CKD, 25 (32.5%) had severe hypertension. The frequency of severe hypertension increased with an increase in IRIS stage; 0% in the controls, 27.6% in the IRIS stage II, and 47.4% in the IRIS stage III-IV, respectively. The indirect SBP was weakly correlated with urea nitrogen (r=0.27) and creatinine (r=0.23) concentrations in plasma. Binary logistic regression analysis showed that if plasma creatinine concentration is >3.7 mg/dl, cats with CKD had an increased risk for developing severe hypertension (P<0.001). Our results suggest that indirect blood pressure was correlated with the severity of CKD, and the prevalence of severe hypertension increased in cats with severe CKD. The risk of severe hypertension may be high in cats with severe CKD.
• The effect of sedation with a combination of butorphanol and midazolam on quantitative contrast-enhanced ultrasonography of duodenum in healthy dogs.
  Nisa K; Lim SY; Osuga T; Yokoyama N; Tamura M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  The Journal of Veterinary Medical Science, 80, 3, 453, 459, Mar. 2018, [Peer-reviewed]
  English, Scientific journal
• Diagnostic accuracy of plasma atrial natriuretic peptide concentrations in cats with and without cardiomyopathies
  Y. Heishima; Y. Hori; K. Nakamura; Y. Yamashita; N. Isayama; N. Kanno; M. Katagi; H. Onodera; S. Yamano; Y. Aramaki
  Journal of Veterinary Cardiology, 20, 4, 234, 243, Elsevier B.V., 2018, [Peer-reviewed]
  English, Scientific journal
• Transcranial Doppler Ultrasound Examination in Dogs with Suspected Intracranial Hypertension Caused by Neurologic Diseases
  K. Sasaoka; K. Nakamura; T. Osuga; T. Morita; N. Yokoyama; K. Morishita; N. Sasaki; H. Ohta; M. Takiguchi
  Journal of Veterinary Internal Medicine, 32, 1, 314, 323, Blackwell Publishing Inc., 01 Jan. 2018, [Peer-reviewed]
  English, Scientific journal
• Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats.
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in veterinary science, 5, 289, 289, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Increased incidence of kidney disease (KD) is a common concern in human and companion animals. Cats, in particular, are highly susceptible to KD. Novel KD biomarkers would help to address these problems. Therefore, we are focusing on microRNA, a highly conserved nucleic acid, as a KD biomarker for various animals. We previously reported that altered levels of urinary exosome (UExo)-derived microRNAs indicate renal pathologies in dogs. This study comprehensively examined UExo-derived microRNAs, which reflected the KD status in cats. The examined cats were divided into two groups: normal renal function (NR) and KD. Based on our previous data in dogs and cats, as well as the present data on UExo-derived microRNAs in cats by next-generation sequencing, let-7b, let-7f, miR-10a, miR-10b, miR-21a, miR-22, miR-26a, miR-27b, miR-146a, miR-181a, miR-191, and miR-486a were identified as biomarker candidates. In summary, the levels of UExo-derived let-7b, miR-22, and miR-26a significantly decreased in cats with KD from the early stages of the disease. UExo-derived miRNA levels normalized to urinary creatinine or total RNA of miR-21a was significantly higher in the KD group. Importantly, the ratio of UExo-derived miR-21a to let-7b showed a significant and strongest correlation with serum creatinine (ρ = 0.751), blood urea nitrogen (ρ = 0.754), and urinary creatinine (ρ = -0.421) among all examined indices. Further, the ratio of miR-181a to let-7b or miR-10b significantly correlated with the progression of renal dysfunction in the KD group. Thus, we identified that UExo-derived microRNAs in cats, and their raw and normalized levels could indicate altered renal function.
• Diastolic heart failure associated with hemangiosarcoma infiltrating left ventricular walls in a dog.
  Tatsuyuki Osuga; Kensuke Nakamura; Tomoya Morita; Yumiko Kagawa; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Canadian veterinary journal = La revue veterinaire canadienne, 58, 11, 1167, 1170, Nov. 2017, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, A 9-year-old Shetland sheepdog was diagnosed with cardiogenic pulmonary edema. Echocardiography revealed focally thickened left ventricular free wall and interventricular septum and left atrial dilation. Left ventricular systolic function was preserved. Doppler echocardiography of transmitral flow indicated restrictive left ventricular filling. Cardiac histopathology demonstrated hemangiosarcoma infiltrating the left ventricular walls.
• Repeatability and reproducibility of quantitative contrast-enhanced ultrasonography for assessing duodenal perfusion in healthy dogs.
  Khoirun Nisa; Sue Yee Lim; Masayoshi Shinohara; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Rommaneeya Leela-Arporn; Tomoya Morita; Nozomu Yokoyama; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 79, 9, 1585, 1590, 29 Sep. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Repeatability and reproducibility of quantitative contrast-enhanced ultrasonography for assessing duodenal perfusion in healthy dogs.
  Nisa K; Lim SY; Shinohara M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Yokoyama N; Osuga T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  The Journal of Veterinary Medical Science, 79, 9, 1585, 1590, Sep. 2017, [Peer-reviewed]
  English, Scientific journal
• The repeatability and characteristics of right ventricular longitudinal strain imaging by speckle-tracking echocardiography in healthy dogs
  T. Morita; K. Nakamura; T. Osuga; N. Yokoyama; N. Khoirun; K. Morishita; N. Sasaki; H. Ohta; M. Takiguchi
  JOURNAL OF VETERINARY CARDIOLOGY, 19, 4, 351, 362, Aug. 2017, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Changes in right ventricular function assessed by echocardiography in dog models of mild RV pressure overload.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Nozomu Yokoyama; Keitaro Morishita; Noboru Sasaki; Hiroshi Ohta; Mitsuyoshi Takiguchi
  Echocardiography (Mount Kisco, N.Y.), 34, 7, 1040, 1049, Jul. 2017, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal
• Stimulation of colorectal biopsies from miniature dachshunds with inflammatory colorectal polyps with toll-like receptor ligands: A pilot study.
  Nozomu Yokoyama; Hiroshi Ohta; Yumiko Kagawa; Noriyuki Nagata; Khoirun Nisa; Tomoya Morita; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Veterinary immunology and immunopathology, 188, 78, 83, Jun. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Serial measurement of pancreatic lipase immunoreactivity concentration in dogs with immune-mediated disease treated with prednisolone
  H. Ohta; T. Morita; N. Yokoyama; T. Osuga; N. Sasaki; K. Morishita; K. Nakamura; M. Takiguchi
  JOURNAL OF SMALL ANIMAL PRACTICE, 58, 6, 342, 347, Jun. 2017, [Peer-reviewed]
  English, Scientific journal
• Washout Ratio in the Hepatic Vein Measured by Contrast-Enhanced Ultrasonography to Distinguish Between Inflammatory and Noninflammatory Hepatic Disorders in Dogs
  K. Morishita; A. Hiramoto; A. Michishita; S. Takagi; T. Osuga; S. Y. Lim; K. Nakamura; N. Sasaki; H. Ohta; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 31, 3, 770, 777, May 2017, [Peer-reviewed]
  English, Scientific journal
• Washout Ratio in the Hepatic Vein Measured by Contrast-Enhanced Ultrasonography to Distinguish Between Inflammatory and Noninflammatory Hepatic Disorders in Dogs
  K. Morishita; A. Hiramoto; A. Michishita; S. Takagi; T. Osuga; S. Y. Lim; K. Nakamura; N. Sasaki; H. Ohta; M. Takiguchi
  Journal of Veterinary Internal Medicine, 31, 3, 770, 777, Blackwell Publishing Inc., 01 May 2017, [Peer-reviewed]
  English, Scientific journal
• Renal failure in dogs kept by a man with severe psoriasis
  Y. Fujita; K. Hanazono; E. Uchida; T. Kawahata; A. Suto; K. Nakamura; H. Shimizu
  JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, 31, 4, E184, E185, Apr. 2017, [Peer-reviewed]
  English
• Assessment of contrast-enhanced ultrasonography of the hepatic vein for detection of hemodynamic changes associated with experimentally induced portal hypertension in dogs.
  Keitaro Morishita; Akira Hiramoto; Asuka Michishita; Satoshi Takagi; Yuki Hoshino; Takaharu Itami; Sue Yee Lim; Tatsuyuki Osuga; Sayuri Nakamura; Kenji Ochiai; Kensuke Nakamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 78, 4, 465, 471, Apr. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Expression of apical junction complex proteins in colorectal mucosa of miniature dachshunds with inflammatory colorectal polyps.
  Nozomu Yokoyama; Hiroshi Ohta; Yumiko Kagawa; Rommaneeya Leela-Arporn; Angkhana Dermlim; Khoirun Nisa; Tomoya Morita; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 79, 3, 456, 463, 18 Mar. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Left Atrial Strain at Different Stages of Myxomatous Mitral Valve Disease in Dogs
  K. Nakamura; S. Kawamoto; T. Osuga; T. Morita; N. Sasaki; K. Morishita; H. Ohta; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 31, 2, 316, 325, Mar. 2017, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Localization of Toll-like Receptor (TLR) 2 and TLR4 mRNA in the Colorectal Mucosa of Miniature Dachshunds with Inflammatory Colorectal Polyps
  N. Yokoyama; H. Ohta; J. Yamazaki; Y. Kagawa; O. Ichii; N. Khoirun; T. Morita; T. Osuga; S. Y. Lim; N. Sasaki; K. Morishita; K. Nakamura; M. Takiguchi
  JOURNAL OF COMPARATIVE PATHOLOGY, 156, 2-3, 183, 190, Feb. 2017, [Peer-reviewed]
  English, Scientific journal
• Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs.
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Yuki Hoshino; Satoshi Takagi; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yasuhiro Kon
  Scientific reports, 7, 40340, 40340, 11 Jan. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Contrast-enhanced ultrasonography of the hepatic vein in normal dogs.
  Keitaro Morishita; Akira Hiramoto; Tatsuyuki Osuga; Sue Yee Lim; Nisa Khoirun; Noboru Sasaki; Kensuke Nakamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 78, 12, 1753, 1758, 10 Jan. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Contrast-enhanced ultrasonography (CEUS) of the hepatic vein can assess intrahepatic hemodynamic changes and has been studied as a noninvasive method to assess the severity of portal hypertension and hepatic fibrosis in humans. However, few reports have described its usefulness in veterinary medicine. The purpose of this study was to characterize CEUS findings of the hepatic vein in normal dogs and assess the repeatability of this method both in a conscious group (n=6) and a sedated group (n=6). Sonazoid® (0.01 ml/kg) was used as a contrast agent, and scanning of the hepatic vein was performed for 2 min. Time-intensity curves were generated from regions of interest in the hepatic vein. Four perfusion parameters were measured for quantitative analysis: hepatic vein arrival time (HVAT), time to peak (TTP), time to peak phase (TTPP) and wash-out ratio (WR). CEUS examinations were performed three times in each dog. The median (range) values of HVAT, TTP, TTPP and WR in the conscious group were 13.5 sec (9-22 sec), 12.5 sec (6-24 sec), 8 sec (6-13 sec) and 78.0% (60.7-91.7%), respectively. Median (range) values of HVAT, TTP, TTPP and WR in the sedated group were 12 sec (8-17 sec), 12.5 sec (9-17 sec), 9 sec (7-13 sec) and 84.1% (63.0-94.4%), respectively. The coefficients of variation of these parameters in the conscious and sedated groups were 7.6-29.7% and 11.8-14.8%, respectively.
• Case report: Congenital methemoglobinemia in a cat with the reduced NADH-cytochrome b5 reductase 3 activity and missense mutations in CYB5R3
  Akiyoshi Tani; Jumpei Yamazaki; Kensuke Nakamura; Mitsuyoshi Takiguchi; Mutsumi Inaba
  Japanese Journal of Veterinary Research, 65, 4, 201, 206, Hokkaido University, 2017, [Peer-reviewed]
  English, Scientific journal
• Spatial and temporal profile of cisplatin delivery by ultrasound-assisted intravesical chemotherapy in a bladder cancer model.
  Noboru Sasaki; Kazuhiro Ishi; Nobuki Kudo; Shouta M M Nakayama; Kensuke Nakamura; Keitaro Morishita; Hiroshi Ohta; Mayumi Ishizuka; Mitsuyoshi Takiguchi
  PloS one, 12, 11, e0188093, 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Impact of surgical margins on survival of 37 dogs with massive hepatocellular carcinoma
  A. Matsuyama; S. Takagi; K. Hosoya; Y. Kagawa; K. Nakamura; T. Deguchi; M. Takiguchi
  NEW ZEALAND VETERINARY JOURNAL, 65, 5, 227, 231, 2017, [Peer-reviewed]
  English, Scientific journal
• Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90.
  Masahiro Yamasaki; Yoshihiro Tsuboi; Yusuke Taniyama; Naohiro Uchida; Reeko Sato; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 78, 8, 1355, 60, 01 Sep. 2016, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length coding sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels were elevated when the parasites were incubated at 43°C for 1 hr.
• Effects of experimental cardiac volume loading on left atrial phasic function in healthy dogs.
  Tatsuyuki Osuga; Kensuke Nakamura; Tomoya Morita; Khoirun Nisa; Nozomu Yokoyama; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  American journal of veterinary research, 77, 9, 952, 60, Sep. 2016, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal, OBJECTIVE To elucidate the relationship between acute volume overload and left atrial phasic function in healthy dogs. ANIMALS 6 healthy Beagles. PROCEDURES Dogs were anesthetized. A Swan-Ganz catheter was placed to measure mean pulmonary capillary wedge pressure (PCWP). Cardiac preload was increased by IV infusion with lactated Ringer solution at 150 mL/kg/h for 90 minutes. Transthoracic echocardiography was performed before (baseline) and at 15, 30, 45, 60, 75, and 90 minutes after volume loading began. At each echocardiographic assessment point, apical 4-chamber images were recorded and analyzed to derive time-left atrial area curves. Left atrial total (for reservoir function), passive (for conduit function), and active (for booster-pump function) fractional area changes were calculated from the curves. RESULTS Volume overload resulted in a significant increase from baseline in PCWP from 15 to 90 minutes after volume loading began. All fractional area changes at 15 to 90 minutes were significantly increased from baseline. In multiple regression analysis, quadratic regression models were better fitted to the relationships between PCWP and each of the total and active fractional area changes than were linear regression models. A linear regression model was better fitted to the relationship between PCWP and passive fractional area change. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that left atrial phasic function assessed on the basis of left atrial phasic areas was enhanced during experimental cardiac volume loading in healthy dogs. The effect of volume load should be considered when evaluating left atrial phasic function by indices derived from left atrial phasic sizes.
• 活性型ビタミンD3軟膏使用中に飼い犬に腎不全をきたした尋常性乾癬の1例
  藤田 靖幸; 須藤 明日香; 清水 宏; 華園 究; 中村 健介; 内田 英二; 川畑 智敬
  日本皮膚科学会雑誌, 126, 9, 1726, 1726, (公社)日本皮膚科学会, Aug. 2016, [Peer-reviewed]
  Japanese, Scientific journal
• Hypercalcemia in a Dog with Chronic Ingestion of Maxacalcitol Ointment
  Kensuke Nakamura; Nobuo Tohyama; Masahiro Yamasaki; Hiroshi Ohta; Keitaro Morishita; Mitsuyoshi Takiguchi
  JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION, 52, 4, 256, 258, Jul. 2016, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Repeatability and reproducibility of right ventricular Tei index valves derived from three echocardiographic methods for evaluation of cardiac function in dogs.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Sue Yee Lim; Nozomu Yokoyama; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  American journal of veterinary research, 77, 7, 715, 20, Jul. 2016, [Peer-reviewed], [Lead author], [International Magazine]
  English, OBJECTIVE To evaluate repeatability and reproducibility of right ventricular Tei index (RTX) values derived from dual pulsed-wave Doppler, conventional pulsed-wave Doppler, and tissue Doppler echocardiography and to investigate relationships and repeatability among the 3 methods in healthy dogs. ANIMALS 6 healthy adult Beagles. PROCEDURE Echocardiography was performed on each dog on different days for 2 weeks (3 times/d) by 2 echocardiographers. Intraobserver within- and between-day and interobserver coefficients of variation (CVs) and intraclass correlation coefficients (ICCs) for RTXs derived from dual pulse-waved Doppler (RTXDPD), conventional pulsed-wave Doppler (RTXPD), and tissue Doppler (RTXTD) methods were determined. Degrees of agreement among RTX values derived from the 3 methods were assessed by modified Bland-Altman analysis. RESULTS Least squares mean (95% confidence interval) RTXtd was 0.50 (0.46 to 0.54), which was significantly higher than that for RTXDPD (0.27 [0.23 to 0.31]) and RTXPD (0.25 [0.21 to 0.29]). Agreement between RTXDPD and RTXPD was good (bias [mean difference], 0.04 [95% confidence interval, -0.03 to 0.10]). The RTXdpd had high within-day (CV, 6.1; ICC, 0.77) and interobserver (CV, 3.5; ICC, 0.83) repeatability, but between-day repeatability was not high. The RTXtd had high within-day repeatability (CV, 6.0; ICC, 0.80), but between-day and interobserver repeatability were not high. Within-day, between-day, and interobserver repeatability of RTXPD were not high. CONCLUSIONS AND CLINICAL RELEVANCE RTXdpd measurement was a repeatable and reproducible method of cardiac evaluation in healthy dogs. The RTXTD values were significantly higher than the RTXDPD and RTXPD values; therefore, RTX values derived from different echocardiographic methods should be interpreted with caution.
• Prognostic Value of Right Ventricular Tei Index in Dogs with Myxomatous Mitral Valvular Heart Disease
  K. Nakamura; T. Morita; T. Osuga; K. Morishita; N. Sasaki; H. Ohta; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 30, 1, 69, 75, Jan. 2016, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Vitamin D Status in Different Stages of Disease Severity in Dogs with Chronic Valvular Heart Disease
  T. Osuga; K. Nakamura; T. Morita; S. Y. Lim; K. Nisa; N. Yokoyama; N. Sasaki; K. Morishita; H. Ohta; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 29, 6, 1518, 1523, Nov. 2015, [Peer-reviewed]
  English, Scientific journal
• Effects of various cardiovascular drugs on indices obtained with two-dimensional speckle tracking echocardiography of the left atrium and time-left atrial area curve analysis in healthy dogs.
  Tatsuyuki Osuga; Kensuke Nakamura; Tomoya Morita; Sue Yee Lim; Nozomu Yokoyama; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  American journal of veterinary research, 76, 8, 702, 9, Aug. 2015, [Peer-reviewed], [Lead author], [International Magazine]
  English, OBJECTIVE: To evaluate the effects of dobutamine, esmolol, milrinone, and phenylephrine on left atrial phasic function of healthy dogs. ANIMALS: 9 healthy Beagles. PROCEDURES: Following sedation with propofol on each of 4 experimental days, dogs were administered a constant rate infusion of dobutamine (5 μg/kg/min), esmolol (500 μg/kg/min), milrinone (25 μg/kg, IV bolus, followed by 0.5 μg/kg/min), or phenylephrine (2 μg/kg/min). There was at least a 14-day interval between experimental days. Each drug was administered to 6 dogs. Conventional and 2-D speckle tracking echocardiography were performed before (baseline) and after administration of the cardiovascular drug, and time-left atrial area curves were derived to calculate indices for left atrial reservoir, conduit, and booster pump functions (left atrial phasic function) and left ventricular contractility and lusitropy. RESULTS: Compared with baseline values, indices for left atrial reservoir and booster pump functions and left ventricular contractility and lusitropy were significantly increased following dobutamine administration; indices for left atrial phasic function and left ventricular lusitropy were changed insignificantly, and indices for left ventricular contractility were significantly impaired following esmolol administration; indices for left atrial phasic function and left ventricular relaxation were changed insignificantly, and indices for left ventricular systolic function were significantly augmented following milrinone administration; and indices for left atrial phasic function and left ventricular lusitropy were changed insignificantly, and indices of ventricular contractility were significantly impaired following phenylephrine administration. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that, following administration of dobutamine, esmolol, milrinone, or phenylephrine to healthy dogs, left atrial phasic function indices were fairly stable and did not parallel changes in left ventricular function indices.
• Quantitative Contrast-enhanced Ultrasonographic Assessment of Naturally Occurring Pancreatitis in Dogs
  S. Y. Lim; K. Nakamura; K. Morishita; N. Sasaki; M. Murakami; T. Osuga; N. Yokoyama; H. Ohta; M. Yamasaki; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 29, 1, 71, 78, Jan. 2015, [Peer-reviewed]
  English, Scientific journal
• CONTRAST-ENHANCED ULTRASONOGRAPHIC FINDINGS IN THREE DOGS WITH PANCREATIC INSULINOMA
  Kensuke Nakamura; Sue-Yee Lim; Kenji Ochiai; Masahiro Yamasaki; Hiroshi Ohta; Keitaro Morishita; Satoshi Takagi; Mitsuyoshi Takiguchi
  VETERINARY RADIOLOGY & ULTRASOUND, 56, 1, 55, 62, Jan. 2015, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Lesion Type Classification by Applying Machine-Learning Technique to Contrast-Enhanced Ultrasound Images
  Kazuya Takagi; Satoshi Kondo; Kensuke Nakamura; Mitsuyoshi Takiguchi
  IEICE TRANSACTIONS ON INFORMATION AND SYSTEMS, E97D, 11, 2947, 2954, Nov. 2014, [Peer-reviewed]
  English, Scientific journal
• Prognostic Value of Left Atrial Function in Dogs with Chronic Mitral Valvular Heart Disease
  K. Nakamura; T. Osuga; K. Morishita; S. Suzuki; T. Morita; N. Yokoyama; H. Ohta; M. Yamasaki; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 28, 6, 1746, 1752, Nov. 2014, [Peer-reviewed], [Lead author]
  English, Scientific journal
• In vitro and in vivo safety and efficacy studies of amphotericin B on Babesia gibsoni.
  Masahiro Yamasaki; Eriko Harada; Yu Tamura; Sue Yee Lim; Tatsuyuki Ohsuga; Nozomu Yokoyama; Keitaro Morishita; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  Veterinary parasitology, 205, 3-4, 424, 33, 15 Oct. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Evaluation of selected cytokine gene expression in colonic mucosa from dogs with idiopathic lymphocytic-plasmacytic colitis.
  Yu Tamura; Hiroshi Ohta; Nozomu Yokoyama; Sue Yee Lim; Tatsuyuki Osuga; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 76, 10, 1407, 10, Oct. 2014, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Case report: protein-losing enteropathy caused by Mesocestoides vogae (syn. M. corti) in a dog.
  Yu Tamura; Hiroshi Ohta; Takuya Kashiide; Jun Matsumoto; Tatsuya Sakurai; Nozomu Yokoyama; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  Veterinary parasitology, 205, 1-2, 412, 5, 15 Sep. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Expression of apical junction complex proteins in duodenal mucosa of dogs with inflammatory bowel disease.
  Hiroshi Ohta; Yuji Sunden; Nozomu Yokoyama; Tatsuyuki Osuga; Sue Yee Lim; Yu Tamura; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 75, 8, 746, 51, Aug. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Qualitative and Quantitative Contrast-Enhanced Ultrasonographic Assessment of Cerulein-Induced Acute Pancreatitis in Dogs
  S. Y. Lim; K. Nakamura; K. Morishita; N. Sasaki; M. Murakami; T. Osuga; H. Ohta; M. Yamasaki; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 28, 2, 496, 503, Mar. 2014, [Peer-reviewed]
  English, Scientific journal
• CD4⁺ T cell cytokine gene and protein expression in duodenal mucosa of dogs with inflammatory bowel disease.
  Hiroshi Ohta; Kanae Takada; Yuji Sunden; Yu Tamura; Tatsuyuki Osuga; Sue Yee Lim; Masahiro Murakami; Noboru Sasaki; Bandula Kumara Wickramasekara Rajapakshage; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 76, 3, 409, 14, Mar. 2014, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Ultrasound image-guided therapy enhances antitumor effect of cisplatin.
  Noboru Sasaki; Nobuki Kudo; Kensuke Nakamura; Sue Yee Lim; Masahiro Murakami; W R Bandula Kumara; Yu Tamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  Journal of medical ultrasonics (2001), 41, 1, 11, 21, Jan. 2014, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, PURPOSE: The aim of this study was to clarify whether ultrasound image-guided cisplatin delivery with an intratumor microbubble injection enhances the antitumor effect in a xenograft mouse model. METHODS: Canine thyroid adenocarcinoma cells were used for all experiments. Before in vivo experiments, the cisplatin and microbubble concentration and ultrasound exposure time were optimized in vitro. For in vivo experiments, cells were implanted into the back of nude mice. Observed by a diagnostic ultrasound machine, a mixture of cisplatin and ultrasound contrast agent, Sonazoid, microbubbles was injected directly into tumors. The amount of injected cisplatin and microbubbles was 1 μg/tumor and 1.2 × 10(7) microbubbles/tumor, respectively, with a total injected volume of 20 μl. Using the same diagnostic machine, tumors were exposed to ultrasound for 15 s. The treatment was repeated four times. RESULTS: The combination of cisplatin, microbubbles, and ultrasound significantly delayed tumor growth as compared with no treatment (after 18 days, 157 ± 55 vs. 398 ± 49 mm(3), P = 0.049). Neither cisplatin alone nor the combination of cisplatin and ultrasound delayed tumor growth. The treatment did not decrease the body weight of mice. CONCLUSION: Ultrasound image-guided anticancer drug delivery may enhance the antitumor effects of drugs without obvious side effects.
• Qualitative and quantitative contrast enhanced ultrasonography of the pancreas using bolus injection and continuous infusion methods in normal dogs.
  Sue Yee Lim; Kensuke Nakamura; Keitaro Morishita; Noboru Sasaki; Masahiro Murakami; Tatsuyuki Osuga; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 75, 12, 1601, 7, 30 Dec. 2013, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Quantitative contrast enhanced ultrasound is a major breakthrough for ultrasound imaging in recent years. However, contrast enhancement of the pancreas is brief with bolus injection. To assess if continuous infusion of Sonazoid(®) can prolong the duration of pancreatic enhancement over bolus injections, eight adult dogs received bolus injection and continuous infusion of Sonazoid(®) on separate days. Contrast enhanced ultrasound of the pancreatic parenchyma and proximal descending duodenum was performed, and time intensity curves reflecting tissue perfusions were generated. Perfusion parameters- time to initial upslope, peak time, time to wash-out and peak intensity were calculated and evaluated. Fast wash-in to intense peak, followed by rapid wash-out was observed for time intensity curves of bolus injection. With continuous infusion, contrast wash-in to peak intensity was gradual, followed by long plateau and slow wash-out. Median contrast enhancement durations of the pancreas and duodenum were significantly prolonged by continuous infusion from 11 sec (range, 10 to 23 sec) and 16 sec (range, 3 to 43 sec) at bolus injection to 205 sec (range, 170 to 264 sec, P<0.01) and 193 sec (range, 169 to 216 sec, P<0.05), respectively. Median peak intensity of the pancreas was 100.9 MPV (range, 80.2 to 124.3 MPV) at bolus injection and 77.6 MPV (range, 58.2 to 99.5 MPV, P<0.05) at continuous infusion. Prolonged continuous imaging is afforded by continuous infusion of contrast agent. Peak intensity of the pancreas was slightly diminished in continuous infusion, but offered adequate imaging subjectively.
• Markedly increased expression of interleukin-8 in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds.
  Yu Tamura; Hiroshi Ohta; Shidow Torisu; Masashi Yuki; Nozomu Yokoyama; Masahiro Murakami; Sue Yee Lim; Tatsuyuki Osuga; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  Veterinary immunology and immunopathology, 156, 1-2, 32, 42, 15 Nov. 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Inflammatory colorectal polyps (ICRPs) in miniature dachshunds were recently recognized as a major cause of large bowel diarrhea in this dog breed in Japan. ICRPs are characterized by the formation of multiple small polyps and/or space-occupying large polyps in the colorectal area and are thought to be a novel form of inflammatory bowel disease (IBD). To explore key mediators in the pathogenesis of ICRPs, we analyzed several pro-inflammatory cytokine (IL-1β, IL-6, TNF-α, IL-8, IL-12p35, IL-12/23p40, and IL-23p19) mRNA expressions in colorectal polyps in ICRP dogs by quantitative PCR. Among these cytokines, IL-8 mRNA expression was markedly up-regulated in large polyps. To examine IL-8 protein expression, we analyzed IL-8 protein level and its location in colorectal mucosal specimens of ICRP dogs by ELISA and immunofluorescence microscopy. IL-8 protein was significantly increased in large polyps and serum in dogs with ICRPs compared to controls. By immunofluorescence microscopy, IL-8 was only localized in macrophages, but not in mucosal epithelial cells or neutrophils. IL-8-positive macrophages were significantly increased in large polyps compared to controls. These results suggest that IL-8 is produced mainly by macrophages and may induce neutrophil infiltration in the colorectal area of ICRP dogs.
• Expression of CD4+ T cell cytokine genes in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds.
  Hiroshi Ohta; Kanae Takada; Shidow Torisu; Masashi Yuki; Yu Tamura; Nozomu Yokoyama; Tatsuyuki Osuga; Sue Yee Lim; Masahiro Murakami; Noboru Sasaki; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  Veterinary immunology and immunopathology, 155, 4, 259, 63, 01 Oct. 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Inflammatory colorectal polyps (ICRPs) in miniature dachshunds are recently recognized as a major cause of large bowel diarrhea in this dog breed in Japan. ICRPs are characterized by the formation of multiple small polyps and a space-occupying large polyp in the colorectal area, and are thought to be a novel form of inflammatory bowel disease (IBD). In humans, specific cytokine patterns attributed to T helper (Th)1, Th17 and regulatory T cells have important roles in the pathogenesis of IBD. Thus, the aim of the present study was to assess the gene expression of cytokines of T cell subsets in the colorectal mucosa from dogs with ICRPs. Colorectal mucosal specimens from 10 dogs with ICRPs and 14 control dogs were used in this study. Interferon (IFN)-γ, interleukin (IL)-4, IL-17A and IL-10 mRNA expression was assessed using quantitative real-time PCR. IL-17A mRNA expression was significantly increased in large polyps compared to small polyps and controls. IFN-γ and IL-10 mRNA expression in large polyps were significantly higher than in controls. There was no significant difference in IL-4 mRNA expression among the three groups. IL-17A is thought to play important roles in the pathogenesis of ICRPs. IL-10 up-regulation could oppose the proinflammatory function of IL-17A.
• Repeatability and reproducibility of measurements obtained via two-dimensional speckle tracking echocardiography of the left atrium and time-left atrial area curve analysis in healthy dogs.
  Tatsuyuki Osuga; Kensuke Nakamura; Sue Yee Lim; Yu Tamura; Wickramasekara Rajapakshage Bandula Kumara; Masahiro Murakami; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 74, 6, 864, 9, Jun. 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To evaluate left atrial phasic function in healthy dogs by means of 2-D speckle tracking echocardiography with time-left atrial area curve analysis and to assess repeatability and reproducibility of obtained measurements. ANIMALS: 6 healthy Beagles. PROCEDURES: Each dog underwent echocardiography twice on different days (3 nonconsecutive examinations/d). Images were analyzed with offline software; area of the left atrium was automatically calculated in each frame throughout the cardiac cycle to derive time-left atrial area curves. Variables used to assess left atrial phasic function (total, passive, and active emptying area and emptying fractions and mean active and total emptying rates) were calculated. Agreement between variables measured via speckle tracking echocardiography and a manual tracing method was assessed with modified Bland-Altman analysis. Within-day and between-day coefficients of variation were determined. RESULTS: Mean ± SD total, passive, and active emptying fractions of the left atrium were 49.8 ± 3.5%, 277 ± 4.0%, and 30.5 ± 4.3%, respectively. Mean ± SD total and active emptying rates were 16.0 ± 2.5 cm(2)/s and 25.1 ± 4.9 cm(2)/s, respectively. Within-day and between-day coefficients of variation were < 20% (range, 0.41% to 16.4%) for all variables except mean active emptying rate (between-day coefficient of variation, 29.2%). Agreement between variables measured via speckle tracking echocardiography and the manual tracing method was good, and differences between methods were nonsignificant. CONCLUSIONS AND CLINICAL RELEVANCE: Evaluation of left atrial phasic function via speckle tracking echocardiography was feasible; repeatability and reproducibility of measurements were adequate in healthy dogs. Studies are needed to determine clinical applicability in canine patients.
• Involvement of mitochondrial genes of Babesia gibsoni in resistance to diminazene aceturate.
  Bandula Kumara Wickramasekara Rajapakshage; Masahiro Yamasaki; Shiang-Jyi Hwang; Noboru Sasaki; Masahiro Murakami; Yu Tamura; Sue Yee Lim; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 74, 9, 1139, 48, 9, Sep. 2012, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The stability of the characteristics of the diminazene aceturate (DA)-resistant B. gibsoni isolate was initially determined in vitro. Part of the DA-resistant B. gibsoni isolate was cultured without DA for 4 weeks, and then newly exposed to 200 ng/ml DA. As a result, this isolate could proliferate the same as the DA-resistant isolate, indicating that the characteristic of DA resistance was stable in the DA-resistant isolate. Additionally, the level of parasitemia in the DA-resistant isolate was comparatively lower than in the wild-type, suggesting that the proliferation potential of the DA-resistant isolate would be lower than that of the wild-type. Subsequently, to investigate the involvement of mitochondrial DNA (mtDNA) in DA resistance in B. gibsoni, the nucleotide sequences and deduced amino acid sequences of mitochondrial genes such as COXI, COXIII, and CYTb genes of the DA-resistant isolate, were compared with those of the wild-type. As a result, these three genes were not altered in the DA-resistant B. gibsoni isolate. Moreover, the transcription levels of COXI, COXIII, and CYTb genes were observed by semi-quantitative RT-PCR. As a result, the gene transcription of those genes in the DA-resistant isolate was not significantly altered. These results indicated that DA did not affect mtDNA directly in DA-resistant B. gibsoni. Thus, it is suggested that mtDNA should not be deeply involved in DA resistance in B. gibsoni.
• Bilateral Segmental Aplasia with Unilateral Uterine Horn Torsion in a Pomeranian Bitch
  Kensuke Nakamura; Masahiro Yamasaki; Tomohiro Osaki; Hiroshi Ohta; Noboru Sasaki; Keisuke Aoshima; Takashi Kimura; Mitsuyoshi Takiguchi
  JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION, 48, 5, 327, 330, Sep. 2012, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Analysis of energy generation and glycolysis pathway in diminazene aceturate-resistant Babesia gibsoni isolate in vitro.
  Bandula Kumara Wickramasekara Rajapakshage; Masahiro Yamasaki; Masahiro Murakami; Yu Tamura; Sue Yee Lim; Tatsuyuki Osuga; Noboru Sasaki; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Japanese journal of veterinary research, 60, 2-3, 51, 61, 2-3, Aug. 2012, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, In our previous study, the level of parasitemia of the diminazene aceturate (DA)-resistant B. gibsoni isolate was continuously lower than that of the wild-type, indicating the possible alteration of energy metabolism in that isolate. Therefore, in the present study, the concentrations of ATP, glucose, lactate, and pyruvate, and the activities of lactate dehydrogenase and pyruvate kinase in the wild-type and DA-resistant isolate of B. gibsoni were measured and compared to investigate the amount of energy generation and the activity of the glycolysis pathway. As a result, the intracellular ATP and glucose concentrations in the DA-resistant B. gibsoni isolate were significantly higher than those in the wild-type. Meanwhile, the concentrations of lactate and pyruvate and the activities of lactate dehydrogenase and pyruvate kinase in the DA-resistant B. gibsoni isolate were not different from those in the wild-type. These results indicated that the DA-resistant B. gibsoni isolate contained a higher ATP concentration than the wild-type, but the activity of the glycolysis pathway was not altered in the DA-resistant B. gibsoni isolate. However, we could not determine the mechanism of the high energy production of the DA-resistant B. gibsoni isolate. Further studies on the energy metabolism of B. gibsoni are necessary to clarify the mechanism of the high energy production in the DA-resistant B. gibsoni isolate.
• CONTRAST AGENT Gd-EOB-DTPA (EOB center dot Primovist (R)) FOR LOW-FIELD MAGNETIC RESONANCE IMAGING OF CANINE FOCAL LIVER LESIONS
  Daisuke Yonetomi; Tsuyoshi Kadosawa; Kenjirou Miyoshi; Yukie Nakao; Emi Homma; Kiwamu Hanazono; Eriko Yamada; Kozo Nakamura; Atsuki Ijiri; Noriyuki Minegishi; Shigeki Maetani; Kazuko Hirayama; Hiroyuki Taniyama; Tetsuya Nakade
  VETERINARY RADIOLOGY & ULTRASOUND, 53, 4, 371, 380, Jul. 2012, [Peer-reviewed]
  English, Scientific journal
• 犬の肝臓腫瘤診断におけるソナゾイド造影超音波検査
  NAKAMURA KENSUKE
  北海道獣医師会雑誌, 56, 2, 37, 41, Feb. 2012, [Invited]
  Japanese, Scientific journal
• Activation of microbubbles by short-pulsed ultrasound enhances the cytotoxic effect of cis-diamminedichloroplatinum (II) in a canine thyroid adenocarcinoma cell line in vitro.
  Noboru Sasaki; Nobuki Kudo; Kensuke Nakamura; Sue Yee Lim; Masahiro Murakami; W R Bandula Kumara; Yu Tamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  Ultrasound in medicine & biology, 38, 1, 109, 18, 1, Jan. 2012, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Ultrasound targeted microbubble destruction has succeeded in delivering drugs and genes. This study was designed to explore characteristics of ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound. Canine thyroid adenocarcinoma cells were exposed to short-pulsed diagnostic ultrasound in the presence of cis-diamminedichloroplatinum (II) (cisplatin) and ultrasound contrast agent Sonazoid(®) microbubbles. The cytotoxic effect of cisplatin was enhanced by short-pulsed diagnostic ultrasound and microbubbles. Incubation time with microbubbles influenced the cytotoxic effect of cisplatin. However, exposure duration did not affect the cytotoxic effect of cisplatin. Therefore, short-pulsed diagnostic ultrasound may activate microbubbles near cells and deliver cisplatin into cells. In addition, activation of microbubbles may be concluded in a short time. Our results suggest that short exposure duration could be potentially sufficient to induce efficient drug delivery by ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound.
• Enhanced Cytotoxic Effect of Cisplatin Using Diagnostic Ultrasound and Microbubbles In Vitro
  Noboru Sasaki; Kensuke Nakamura; Masahiro Murakami; Sue Yee Lim; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  11TH INTERNATIONAL SYMPOSIUM ON THERAPEUTIC ULTRASOUND, 1481, 307, 310, 2012, [Peer-reviewed]
  English, International conference proceedings
• Effects and mechanisms of action of polyene macrolide antibiotic nystatin on Babesia gibsoni in vitro.
  Masahiro Yamasaki; Norihisa Tamura; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Wickramasekara Rajapakshage; Bandula Kumara; Yu Tamura; Sue Yee Lim; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of parasitology, 97, 6, 1190, 2, 6, Dec. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Nystatin is a membrane-active polyene macrolide antibiotic and a channel-forming ionophore. Nystatin exhibits in vitro activity against Babesia gibsoni infecting normal canine erythrocytes containing low potassium (LK) and high sodium concentrations, i.e., LK erythrocytes. The calculated IC(50) value of nystatin against B. gibsoni infecting LK erythrocytes was 31.96 µg/ml. The anti-babesial activity of nystatin disappeared when B. gibsoni in LK erythrocytes were incubated in culture media containing high potassium concentrations (HK). Moreover, when the parasites were harbored in canine HK erythrocytes, which contained high potassium and low sodium concentrations as a result of high Na-K-ATPase activity, the in vitro anti-babesial activities of nystatin also disappeared, apparently due to protection by HK erythrocytes. This suggested that nystatin could show in vitro anti-babesial activity against B. gibsoni by its ionophorous activity, the same as other ionophores such as valinomycin. Subsequently, the effects of nystatin on the host cells were observed. Nystatin could not modify the intracellular concentrations of potassium, sodium, adenosine triphosphate, or glucose in either LK or HK erythrocytes, although it caused weak hemolysis in HK erythrocytes. In addition, nystatin did not affect the survival of canine peripheral polymorphonuclear leukocytes. In conclusion, nystatin destroyed B. gibsoni by ionophorous activity but did not affect either canine erythrocytes or leukocytes in vitro.
• Effects of sildenafil citrate on five dogs with Eisenmenger's syndrome
  K. Nakamura; M. Yamasaki; H. Ohta; N. Sasaki; M. Murakami; W. R. Bandula Kumara; M. Takiguchi
  JOURNAL OF SMALL ANIMAL PRACTICE, 52, 11, 595, 598, Nov. 2011, [Peer-reviewed], [Lead author]
  English, Scientific journal
• 病理組織検査にて角層下および真皮への多数の好酸球の浸潤が認められた犬の2症例
  CHIBA ERI; YAMASAKI MASAHIRO; OTA HIROSHI; NAKAMURA KENSUKE; SASAKI NOBORU; MURAKAMI MASAHIRO; TAMURA YU; YEE LIM SUE; KUMARA WICKRAMASEKARA R.B; HONDA HIDETAKA; NASUNO TOYOHIKO; OCHIAI KENJI; TAKIGUCHI MITSUYOSHI
  獣医臨床皮膚科, 17, 3, 177, 182, (一社)日本獣医皮膚科学会, Sep. 2011, [Peer-reviewed]
  Japanese
• Detection of anti-Babesia gibsoni heat shock protein 70 antibody and anti-canine heat shock protein 70 antibody in sera from Babesia gibsoni-infected dogs.
  Masahiro Yamasaki; Mikiko Ishida; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Wickramasekara Rajapakshage Bandula Kumara; Yu Tamura; Sue Yee Lim; Hiroshi Ohta; Mitsuyoshi Takiguchi
  Veterinary parasitology, 180, 3-4, 215, 25, 3-4, 25 Aug. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Antibodies that recognized either Babesia gibsoni or canine red blood cell (RBC) 70-kilodalton (kDa) protein were detected in serum from acutely and chronically B. gibsoni-infected. In those sera, antibodies that reacted with recombinant B. gibsoni and canine heat shock protein 70 (rBgHsp70 and rcHsp70) were detected; therefore, B. gibsoni and canine RBC 70-kDa proteins seemed to be BgHsp70 and cHsp70, respectively. In infected dogs, the amounts of these antibodies increased after infection. Interestingly, polyclonal antibody raised against rBgHsp70 in two rabbits reacted not only with rBgHsp70 but also with rcHsp70 and native cHsp70 from canine RBCs. Because BgHsp70 showed high homology with cHsp70 (70.8%), anti-rBgHsp70 antibody might cross-react with cHsp70. Additionally, the localizations of both BgHsp70 and cHsp70 were observed by indirect fluorescence assay. As a result, cHsp70 was not found on the membrane surface of erythrocytes, suggesting that erythrocytes would not be targets of anti-cHsp70 antibody. Meanwhile, only exoerythrocytic parasites were stained by anti-rBgHsp70 antibody. This result showed that BgHsp70 would be expressed on the surface of parasites during the exoerythrocytic stage. These results indicated that BgHsp70 was a highly immunogenic protein in canine B. gibsoni infection, and that exoerythrocytic parasites might be targets of anti-BgHsp70 antibody.
• Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa.
  Hiroshi Ohta; Tomoki Yamaguchi; B K Wickramasekara Rajapakshage; Masahiro Murakami; Noboru Sasaki; Kensuke Nakamura; Shiang-Jyi Hwang; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 72, 8, 1046, 51, 8, Aug. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To examine the expression and distribution of tight junction (TJ) and adherens junction (AJ) proteins in canine duodenal and colonic mucosa. SAMPLE: Mucosa obtained from 4 healthy Beagles. PROCEDURES: Biopsy specimens of the duodenum and colon were obtained via endoscopy from 4 healthy dogs. The expression patterns and subcelluar localization of claudin-1, -2, -3, -4, -5, -7, and -8; E-cadherin; and β-catenin in the duodenum and colon were analyzed by use of immunoblotting and immunofluorescence microscopy. RESULTS: In the duodenum, there was clear expression of claudin-3 and -5, E-cadherin, and β-catenin proteins and weak expression of claudin-7 protein. In contrast, there was clear expression of claudin-2 and -3, E-cadherin, and β-catenin proteins and weak expression of claudin-5 and -7 proteins in the colon, as determined by use of immunoblotting. As determined by the use of immunofluorescence microscopy, the duodenum and colon had staining for claudin-3 and -5, E-cadherin, and β-catenin in the most apical region and staining for claudin-7 in the basolateral region. Staining for claudin-2 was also observed in the colon. CONCLUSIONS AND CLINICAL RELEVANCE: Information was provided about the expression patterns of TJ and AJ proteins in the duodenum and colon of clinically normal dogs. These results may provide valuable information for use in evaluating the importance of these TJ and AJ proteins in the pathogenesis of inflammatory bowel disease in dogs.
• Babesia gibsoni: detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis.
  Masahiro Yamasaki; Yusuke Kobayashi; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Bandula Kumara Wickramasekara Rajapakshage; Hiroshi Ohta; Osamu Yamato; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  Experimental parasitology, 127, 1, 119, 26, 1, Jan. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal, In this study, we attempted to detect Babesia gibsoni in blood smears and formalin-fixed, paraffin-embedded tissues obtained from B. gibsoni-infected dogs using in situ hybridization. Using a digoxigenin-conjugated deoxyribonucleic acid (DNA) probe, both intraerythrocytic and exoerythrocytic parasites in the culture could be specifically stained in blood smears fixed with 4% phosphate-buffered paraformaldehyde. This indicated that genomic DNA extracted from the parasites could be detected using in situ hybridization. Moreover, the parasite could be specifically stained in paraffin-embedded spleen, lymph node, and kidney sections using in situ hybridization. Infected erythrocytes in blood vessels in the spleen and kidney, hemosiderin-laden macrophages in the spleen, and phagocytized erythrocytes, which seemed to be infected with the parasites, in lymph nodes were also specifically stained. This suggests that in situ hybridization can be utilized to investigate both the life cycle of B. gibsoni and the pathological condition of canine babesiosis.
• Contrast-Enhanced Ultrasonography for Characterization of Focal Splenic Lesions in Dogs
  K. Nakamura; N. Sasaki; M. Murakami; W. R. Bandula Kumara; H. Ohta; M. Yamasaki; S. Takagi; T. Osaki; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 24, 6, 1290, 1297, Nov. 2010, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Reduced transcript levels of the heat shock protein 70 gene in diminazene aceturate-resistant Babesia gibsoni variants under low concentrations of diminazene aceturate.
  Shiang-Jyi Hwang; Masahiro Yamasaki; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Bandula Kumara; Wickramasekara Rajapakshage; Hiroshi Ohta; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  The Japanese journal of veterinary research, 58, 3-4, 155, 64, 3-4, Nov. 2010, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, In our previous report, we developed a diminazene aceturate (DA)-resistant Babesia gibsoni strain that was maintained in culture with 200 ng/ml DA. While developing this strain, we also obtained DA-resistant B. gibsoni variants, which were maintained in culture with DA from 1 to 175 ng/ml for more than 8 weeks. Because heat shock protein 70 (Hsp70) seems to play important roles in adaptation to a stress environment in protozoan parasites, in the present study, we examined the copy number of B. gibsoni Hsp70 (BgHsp70) transcripts of those DA-resistant variants using quantitative real-time reverse transcription-polymerase chain reaction. We found that when wild-type B. gibsoni was exposed to 1 ng/ml DA, the level of BgHsp70 transcripts was decreased at day 14. The copy number of BgHsp70 transcripts in the DA-resistant variant cultured with 1 ng/ml DA was significantly lower than in wild-type B. gibsoni, while those in DA-resistant variants increased with escalating doses of DA from 1 to 75 ng/ml, although they were lower than in wild-type B. gibsoni. However, those in DA-resistant variants cultured with >125 ng/ml DA were almost the same as wild-type B. gibsoni. These results indicated that the transcript levels of the BgHsp70 gene might be reduced when the parasites are exposed to a low concentration of DA, and then might recover to the normal level after achieving resistance against DA. We expect that further study of the function of BgHsp70 will elucidate the mechanism of drug resistance against DA in B. gibsoni.
• Development and characterization of a strain of Babesia gibsoni resistant to diminazene aceturate in vitro.
  Shiang-Jyi Hwang; Masahiro Yamasaki; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Bandula Kumara Wickramasekara Rajapakshage; Hiroshi Ohta; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 72, 6, 765, 71, 6, Jun. 2010, [Peer-reviewed], [Domestic magazines]
  English, We attempted to develop a strain of Babesia gibsoni resistant to diminazene aceturate (DA), an anti-babesial drug, in vitro. Since the DA-sensitive B. gibsoni strain could survive and proliferate in culture medium containing 1 ng/m l DA, the concentration of DA was gradually increased from 1 to 200 ng/ml. The results showed that the parasites could survive and proliferate in the medium containing 200 ng/m l DA, which was much higher than the 50% inhibitory concentration (IC(50)) of DA for B. gibsoni. Subsequently, these parasites were removed from erythrocytes and exposed directly to 200 ng/ml DA. They were able to survive and invade fresh erythrocytes, though the DA-sensitive B. gibsoni strain did not survive. Based on these results, the parasites cultured within 200 ng/ml DA were determined to be a DA-resistant B. gibsoni strain. In addition, the IC(50) levels of clindamycin, doxycycline and pentamidine for the DA-resistant B. gibsoni strain were determined. The IC(50) levels of clindamycin, doxycycline and pentamidine for the DA-resistant strain were higher than those for the DA-sensitive strain. The IC(50) of pentamidine for the resistant strain was much greater than that for the DA-sensitive strain. These results indicated that the DA-resistant B. gibsoni strain could have resistance not only to DA, but also to other anti-babesial drugs. In conclusion, we successfully developed a DA-resistant B. gibsoni strain in vitro.
• CONTRAST-ENHANCED ULTRASONOGRAPHY FOR CHARACTERIZATION OF CANINE FOCAL LIVER LESIONS
  Kensuke Nakamura; Satoshi Takagi; Noboru Sasaki; Wickramasekara Rajapakshage Bandula Kumara; Masahiro Murakami; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY RADIOLOGY & ULTRASOUND, 51, 1, 79, 85, Jan. 2010, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Effects and mechanisms of action of ionophorous antibiotics valinomycin and salinomycin-Na on Babesia gibsoni in vitro.
  Masahiro Yamasaki; Kensuke Nakamura; Norihisa Tamura; Shiang-Jyi Hwang; Muneyoshi Yoshikawa; Noboru Sasaki; Hiroshi Ohta; Osamu Yamato; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  The Journal of parasitology, 95, 6, 1532, 8, 6, Dec. 2009, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Valinomycin and salinomycin-Na, 2 ionophorous antibiotics, exhibited in vitro antibabesial activities against Babesia gibsoni that infected normal canine erythrocytes containing low potassium (LK) and high sodium concentrations, i.e., LK erythrocytes, which completely lack Na,K-ATPase activity. The level of parasitemia of B. gibsoni was significantly decreased when the parasites were incubated in culture medium containing either 10(-1) ng/ml valinomycin or 10(2) ng/ml salinomycin-Na for 24 hr. Four-hour incubation in the culture medium containing 5 μg/ml salinomycin-Na led to the destruction of most parasites. In contrast, when the parasites infected canine erythrocytes containing high potassium (HK) and low sodium concentrations, i.e., HK erythrocytes, the in vitro antibabesial activities of both ionophorous antibiotics seemed to be weakened, apparently due to the protection by the host cells. Therefore, differential influences of ionophorous antibiotics on LK and HK erythrocytes were observed. In LK erythrocytes, the intracellular concentrations of potassium, sodium, and adenosine triphosphate (ATP) were not modified, and hemolysis was not observed after incubation in the medium containing each ionophorous antibiotic. These results suggested that these ionophorous antibiotics did not affect cells without Na,K-ATPase, and directly affected B. gibsoni. In HK erythrocytes, the ionophorous antibiotics increased the intracellular sodium concentration, and decreased the intracellular potassium and ATP concentrations, causing obvious hemolysis. Additionally, the decrease of the intracellular ATP concentration and the hemolysis in HK erythrocytes caused by valinomycin disappeared when the activity of Na,K-ATPase was inhibited by ouabain. These results indicate that modification of the intracellular cation concentrations by the ionophorous antibiotics led to the activation of Na,K-ATPase and increased consumption of intracellular ATP, and that the depletion of intracellular ATP resulted in hemolysis in HK erythrocytes. Moreover, the antibabesial activity of valinomycin disappeared when B. gibsoni in LK erythrocytes were incubated in culture media containing high potassium concentrations. This showed that the intracellular cation concentration in the parasites was not modified in those media and would remain the same.
• Primary splenic torsion in a Boston terrier.
  Hiroshi OHTA; Satoshi TAKAGI; Masahiro MURAKAMI; Noboru SASAKI; Muneyoshi YOSHIKAWA; Kensuke NAKAMURA; Shiang-Jyi HWANG; Masahiro YAMASAKI; Mitsuyoshi TAKIGUCHI
  The Journal of veterinary medical science, 71, 11, 1533, 5, 11, Nov. 2009, [Peer-reviewed], [Domestic magazines]
  English, A 7-year-old female Boston terrier was referred to Hokkaido University Veterinary Teaching Hospital with a history of hemoglobinuria and anemia for several days. Abdominal radiographs showed splenomegaly, and ultrasonography revealed a hypoechoic splenic parenchyma with interspersed linear echoes consistent with the ultrasonographic appearance of splenic torsion. Ultrasonography and computed tomography (CT) indicated a C-shaped spleen. Exploratory laparotomy confirmed the diagnosis of splenic torsion. A splenectomy was performed, and the dog recovered well without complications. This is the first report of splenic torsion in Boston terriers, and the usefulness of ultrasonographic and CT findings of the splenic torsion was also confirmed.
• QUANTITATIVE CONTRAST-ENHANCED ULTRASONOGRAPHY OF CANINE SPLEEN
  Kensuke Nakamura; Noboru Sasaki; Muneyoshi Yoshikawa; Hiroshi Ohta; Shiang-Jyi Hwang; Takahiro Mimura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY RADIOLOGY & ULTRASOUND, 50, 1, 104, 108, Jan. 2009, [Peer-reviewed], [Lead author]
  English, Scientific journal

• 臓器・疾患別 最新の治療ガイドライン 循環器疾患 心タンポナーデの原因と対処
  川元誠; 川元誠; 川元誠; 中村健介, 新伴侶動物治療指針, 4, 2025
• 肺高血圧症を併発した粘液腫様変性僧帽弁疾患の犬に対する僧帽弁形成術の短期治療成績
  川元誠; 川元誠; 中村健介; 塩原希; 佐藤景; 村田芙花; 池田正悟; 滝口満喜, 北海道獣医師会雑誌, 69, 8, 2025
• 持続性心房静止と診断した犬の1例
  塩原希; 中村健介; 川元誠; 滝口満喜, 北海道獣医師会雑誌, 69, 8, 2025
• どれを使う? 同効薬・類似薬の使い分けのヒント(第1回)循環器疾患 : 利尿薬
  森田 智也; 中村 健介, CAP : companion animal practice : コンパニオン・アニマル・プラクティス, 39, 8, 34, 39, Aug. 2024
  東京 : チクサン出版社, Japanese
• モデル犬を用いた肺高血圧症を診断するための心エコー図指標の有用性の検証
  森田智也; 森田智也; 中村健介; 大菅辰幸; 川元誠; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 121st, 2024
• 猫の心筋症における左房ブースターストレインの有用性の検討
  佐藤景; 中村健介; 川元誠; 菅原芽伊; 笹岡一慶; 横山望; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 121st, 2024
• 大動脈小体腫瘍の心膜転移により収縮性心膜炎を呈した犬の1例
  塩原希; 中村健介; 細谷謙次; 新坊弦也; 賀川由美子; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 120th, 2024
• 殺細胞効果を有する液体の膀胱内注入治療法の検討
  佐々木東; 池中良徳; 安井博宣; 中村健介; 滝口満喜, 日本獣医学会学術集会講演要旨集, 167th, 2024
• RAISING法を用いたネコ白血病ウイルスの挿入位置決定技術の開発
  佐藤真; 久保田渉誠; 相原浩揮; 直亨則; 斎藤益満; 中村健介; 山崎淳平; 松平崇弘, 日本獣医学会学術集会講演要旨集, 167th, 2024
• How do you treat? Cardiovascular problems associated with endocrine disorders
  中村健介, 動物臨床医学会年次大会プロシーディング, 45th, 1, 2024
• Let’s diagnose stage B2 of mitral regurgitation with echocardiography
  中村健介, 動物臨床医学会年次大会プロシーディング, 45th, 1, 2024
• 咳嗽の実際 症例報告〈2〉:僧帽弁形成術により咳嗽が消失した気管虚脱・気管支軟化症の犬の一例
  川元誠; 川元誠; 川元誠; 中村健介, Veterinary Board, 5, 6, 2023
• 造影超音波検査で副腎皮質の血流消失を確認した医原性副腎皮質機能低下症の犬の1例
  永田矩之; 前田貞俊; 青島圭佑; 中村健介; 滝口満喜, 日本獣医師会獣医学術学会年次大会講演要旨集, 2023 (Web), 2023
• 犬においてピモベンダンとドブタミンを併用することにはどのような意味があるのか?
  大菅辰幸; 大菅辰幸; 矢口紗羅; 中村健介; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 118th, 2023
• 心疾患の犬におけるShear wave elastography/Dispersion Imagingの有用性の検討
  塩原希; 中村健介; 横山望; 佐々木東; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 119th, 2023
• 両側性に発生した副腎皮質腺癌を切除した犬の1例
  藤本晋輔; 鳥巣至道; 中谷圭佑; 保坂悠歩; 金子泰之; 中村健介, 日本獣医師会獣医学術学会年次大会講演要旨集, 2023 (Web), 2023
• 造影超音波検査で副腎皮質の血流消失を確認した医原性副腎皮質機能低下症の犬の1例
  永田矩之; 前田貞俊; 青島圭佑; 中村健介; 滝口満喜, 日本産業動物獣医学会(中部)・日本小動物獣医学会(中部)・日本獣医公衆衛生学会(中部), 2023, 2023
• 犬の発作性ジスキネジア23例の回顧的検討
  川上侑記; 笹岡一慶; 大田寛; 山根由久; 新坊弦也; 横山望; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 67, 8, 2023
• 2次診療動物病院に来院した犬の糞便真核生物叢解析
  横山 望; 川口 剛; 中尾 亮; 森下 啓太郎; 細谷 謙次; 永田 矩之; 笹岡 一慶; 新坊 弦也; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 165回, [HS1P, 16], Sep. 2022
  (公社)日本獣医学会, Japanese
• 悩ましい猫の肺炎vs.肺水腫vs.肺腫瘍 症例報告1):うっ血性心不全により呼吸困難に陥った猫の一例
  川元誠; 川元誠; 川元誠; 中村健介, Veterinary Board, 4, 7, 2022
• 犬の粘液腫様僧帽弁疾患に対する僧帽弁形成術後の左房リバースリモデリングの長期過程と予測因子の検討
  川元誠; 川元誠; 川元誠; 中村健介; 森拓也; 森拓也; 和田智樹; 和田智樹; 藤原彬; 藤原彬; 大橋美里; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 117th, 2022
• 小動物領域における不完全型房室中隔欠損症に対する外科的整復の術式の検討
  森拓也; 森拓也; 森拓也; 古越真耶; 古越真耶; 四井田英樹; 四井田英樹; 大橋美里; 大橋美里; 三木悠矢; 三木悠矢; 田中翔; 田中翔; 和田智樹; 和田智樹; 藤原彬; 藤原彬; 川元誠; 川元誠; 川元誠; 三村貴大; 三村貴大; 野尻麻衣; 野尻麻衣; 武村亮佑; 武村亮佑; 棚橋智彦; 棚橋智彦; 中村健介, 日本獣医循環器学会抄録(CD-ROM), 117th, 2022
• 二度目の肺血栓塞栓症により死亡した腎細胞癌の猫の1例
  湯本優希; 中村健介; 青島圭佑; 小林麻衣; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 117th, 2022
• 猫の超音波検査 パーフェクトアトラス(第19回)不整脈源性右室心筋症
  中村 健介; 森田 智也, Clinic note : clinical daily treatment for the small animal practitioner, 17, 12, 59, 69, Dec. 2021
  インターズー, Japanese
• 犬の僧帽弁疾患における2D Speckle Tracking心エコー図法により推定した左心房stiffnessと予後の関係
  大菅 辰幸; 黒田 杏子; 森田 智也; 中村 健介; 佐々木 東; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 8], Sep. 2021
  (公社)日本獣医学会, Japanese
• Comprehensive analysis of steroid profile in dogs with Cushing' syndrome and hepatocellular carcinoma(和訳中)
  Oo Thandar; 佐々木 東; 池中 良徳; 永田 矩之; 一瀬 貴大; Bin Teoh Yong; 川元 誠; 小島 一輝; 菅原 芽伊; 中山 翔太; 石塚 真由美; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 18], Sep. 2021
  (公社)日本獣医学会, English
• イヌの肝細胞腫瘍におけるDNAメチル化の網羅的解析
  淺利 友; 山崎 淳平; Oo Thander; 細谷 謙次; 金 尚昊; 木之下 怜平; 竹内 恭介; 賀川 由美子; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 30], Sep. 2021
  (公社)日本獣医学会, Japanese
• Identification of pathogenic Single Nucleotide Polymorphism in Inflammatory Colorectal Polyps of Miniature Dachshunds(和訳中)
  Bin Teoh Yong; 永田 矩之; 大田 寛; 佐々木 東; 中村 健介; 村上 正晃; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 32], Sep. 2021
  (公社)日本獣医学会, English
• 非再生性免疫介在性貧血の犬における脾臓摘出術前後の血清中蛋白の網羅的解析
  菅原 芽伊; 山崎 淳平; 森下 啓太郎; 金 尚昊; 細谷 謙次; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 60], Sep. 2021
  (公社)日本獣医学会, Japanese
• 左室壁肥厚と動的流出路閉塞を呈した犬の3例
  湯本 優希; 中村 健介; 志波 一恵; 寺澤 元子; 早坂 郁人; 滝口 満喜, 北海道獣医師会雑誌, 65, 8, 263, 263, Aug. 2021
  (公社)北海道獣医師会, Japanese
• 猫の超音波検査 パーフェクトアトラス(第12回)大腸の腫瘍 大腸性下痢の猫
  中村 健介; 横山 望, Clinic note : clinical daily treatment for the small animal practitioner, 17, 5, 53, 64, May 2021
  インターズー, Japanese
• 超音波で生体の壁を超える 超音波と気泡を併用する膀胱内注入療法の犬における安全性検討
  佐々木 東; 青柳 貞一郎; 池中 良徳; 工藤 信樹; 中村 健介; 滝口 満喜, 超音波医学, 48, Suppl., S195, S195, Apr. 2021
  (公社)日本超音波医学会, Japanese
• Speckle tracking法による右心房機能評価は慢性僧帽弁疾患犬において有用な指標となるか
  森田智也; 森田智也; 中村健介; 大菅辰幸; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 114th, 2021
• 動的左室流出路閉塞の犬4例
  湯本優希; 中村健介; 志波一恵; 寺澤元子; 幸野瑠美; 早坂郁人; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 115th, 2021
• メラトニンの経口投与が著効した両側性副腎腫大を伴う全身性脱毛症のフェレットの1例
  BIN Teoh Yong; 亀山健吾; 永田矩之; 佐々木東; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 65, 8, 2021
• Safety aspects of ultrasound-assisted intravesical chemotherapy in dogs
  佐々木東; 青柳貞一郎; 池中良徳; 工藤信樹; 中村健介; 滝口満喜, 超音波医学 Supplement, 48, 2021
• 症例報告 肺高血圧症を呈した症例 (Feature Article 呼吸器 : 肺が白い!)
  森田 智也; 中村 健介, Veterinary board : 臨床の選択肢を広げるケーススタディ・マガジン, 2, 8, 46, 53, Aug. 2020
  インターズー, Japanese
• 【猫の肥大型心筋症】開口呼吸を主訴に来院した左室中部狭窄の一例
  中村 健介, Veterinary Board, 2, 2, 16, 21, Feb. 2020
  (株)インターズー, Japanese
• 【猫の肥大型心筋症】慢性気管支炎と心不全を併発したHCMの一例
  和田 正太; 中村 健介, Veterinary Board, 2, 2, 22, 30, Feb. 2020
  (株)インターズー, Japanese
• 【猫の肥大型心筋症】心臓形態の経時的変化が観察されたHCM猫
  森田 智也; 中村 健介, Veterinary Board, 2, 2, 32, 40, Feb. 2020
  (株)インターズー, Japanese
• 診療力が試される症例 Vet Board QUIZ 肝・胆道系・膵外分泌 黄疸、腹水、血小板減少、低アルブミン血症が認められたキャバリア・キング・チャールズ・スパニエル
  田村 昌大; 中村 健介, Veterinary Board, 2, 1, 56, 62, Jan. 2020
  (株)インターズー, Japanese
• 基礎からおさえる呼吸器診療の最前線(第18回) 肺血栓塞栓症
  中村 健介, CAP: Companion Animal Practice, 34, 12, 52, 60, Dec. 2019
  (株)緑書房, Japanese
• 【最新輸液療法〜症例から正しい輸液の概念を見直す〜】(Vol.6)慢性腎臓病の輸液管理
  高田 佳苗; 中村 健介, SA Medicine, 21, 6, 29, 47, Dec. 2019
  (株)インターズー, Japanese
• ケースレポートの書き方
  中村 健介, 日本獣医麻酔外科学雑誌, 50, Suppl.2, 173, 173, Dec. 2019
  (一社)日本獣医麻酔外科学会, Japanese
• 胸部レントゲン、エコー、CT検査の比較検討 レントゲン、エコー検査
  中村 健介, 動物臨床医学会年次大会プロシーディング, 40回, 1, 235, 235, Nov. 2019
  動物臨床医学会, Japanese
• 僧帽弁閉鎖不全症の新たな治療選択肢 ベトメディン注射液 東西解説セミナー
  中村 健介, 動物臨床医学会年次大会プロシーディング, 40回, 3, 138, 138, Nov. 2019
  動物臨床医学会, Japanese
• 基礎からおさえる呼吸器診療の最前線(第14回) 間質性肺疾患
  中村 健介, CAP: Companion Animal Practice, 34, 8, 47, 57, Aug. 2019
  (株)緑書房, Japanese
• 診療力が試される症例 循環器 Vet Board QUIZ 問診直後に突然虚脱した僧帽弁逆流 stage B2だったはずのチワワ
  中村 健介, Veterinary Board, 1, 4, 55, 59, Aug. 2019
  (株)インターズー, Japanese
• 基礎からおさえる呼吸器診療の最前線(第13回) 誤嚥性肺炎
  中村 健介, CAP: Companion Animal Practice, 34, 7, 47, 54, Jul. 2019
  (株)緑書房, Japanese
• 基礎からおさえる呼吸器診療の最前線(第12回) 感染性肺炎
  中村 健介; 藤田 道郎, CAP: Companion Animal Practice, 34, 6, 62, 69, Jun. 2019
  (株)緑書房, Japanese
• 慢性僧帽弁疾患犬において心室間流入時間差は有用な心機能評価指標である
  森田 智也; 中村 健介; 大菅 辰幸; 滝口 満喜, 日本獣医麻酔外科学雑誌, 50, Suppl.1, 322, 322, Jun. 2019
  (一社)日本獣医麻酔外科学会, Japanese
• 【胆嚢疾患に対する選択肢を広げる】胆嚢疾患 総論
  中村 健介, Veterinary Board, 1, 1, 6, 19, May 2019
  (株)インターズー, Japanese
• 【胆嚢疾患に対する選択肢を広げる】大十二指腸乳頭狭窄による肝外胆管閉塞
  向山 徹; 中村 健介; 鳥巣 至道, Veterinary Board, 1, 1, 47, 52, May 2019
  (株)インターズー, Japanese
• 慢性僧帽弁疾患犬において心室間流入時間差は有用な心機能評価指標である
  森田智也; 中村健介; 大菅辰幸; 大菅辰幸; 滝口満喜, 日本獣医麻酔外科学雑誌(Web), 50, Supplement 1, 2019
• 肺高血圧症の診断と右心系機能との関連
  中村 健介, 日本獣医麻酔外科学雑誌, 49, Suppl.2, 144, 144, Dec. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• ピモベンダンは慢性塞栓性肺高血圧症において圧を上げずに拍出量を上げる
  森田 智也; 中村 健介; 大菅 辰幸; 滝口 満喜, 日本獣医麻酔外科学雑誌, 49, Suppl.2, 242, 242, Dec. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• 右心疾患犬における超音波検査を用いた後大静脈指標の有用性の検討
  藤岡 崇伯; 中村 健介; 都築 直; 山口 潤; 日高 勇一, 日本獣医麻酔外科学雑誌, 49, Suppl.2, 249, 249, Dec. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• 部位別の基本描出シリーズ 呼吸器編 4)肺 CT検査
  中村健介, 伴侶動物画像診断, 3, 6, 45, 51, Dec. 2018
  (株)緑書房, Japanese
• 部位別の基本描出シリーズ 呼吸器編 4)肺 X線検査
  中村健介, 伴侶動物画像診断, 3, 6, 6, 32, Dec. 2018
  (株)緑書房, Japanese
• 部位別の基本描出シリーズ 呼吸器編 4)肺 超音波検査
  中村健介, 伴侶動物画像診断, 3, 6, 33, 44, Dec. 2018
  (株)緑書房, Japanese
• 部位別の基本描出シリーズ 呼吸器編 4)肺 症例編
  中村健介, 伴侶動物画像診断, 3, 6, 52, 63, Dec. 2018
  (株)緑書房, Japanese
• 心エコーにて右心系の評価をどう行うか?
  中村 健介, 動物臨床医学会年次大会プロシーディング, 39回, 1, 35, 35, Nov. 2018
  動物臨床医学会, Japanese
• 腹部超音波検査とCT検査の比較検討 腹部超音波検査
  中村 健介, 動物臨床医学会年次大会プロシーディング, 39回, 1, 219, 220, Nov. 2018
  動物臨床医学会, Japanese
• 三尖弁閉鎖不全症の診断と治療 4 右室圧の上昇に伴う三尖弁閉鎖不全症
  中村健介, Veterinary Circulation, 7, 4, 30, 42, Nov. 2018
  (株)インターズー, Japanese
• 健康な犬における二次元スペックルトラッキング心エコーによる左心室長軸方向ストレイン解析に与える心臓容積負荷の影響(Effect of cardiac volume loading on left atrial longitudinal strain analysis derived from two-dimensional Speckle Tracking Echocardiography in healthy dogs)
  Dermlim Angkhana; 中村 健介; 森田 智也; 大菅 辰幸; Nisa Khoirun; Leela-arporn Rommaneeya; 笹岡 一慶; 永田 矩之; 田村 昌大; 佐々木 東; 大田 寛; 森下 啓太郎; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 161回, 435, 435, Aug. 2018
  (公社)日本獣医学会, English
• 【臨床徴候シリーズvol.3 発咳】 肺炎
  中村 健介, J-VET, 31, 8, 39, 45, Aug. 2018
  (株)インターズー, Japanese
• 腫瘍随伴症候群~知る!診る!治す!そして活かす!高コルチゾール血症
  中村健介, Veterinary Oncology, 5, 3, 63, 69, Jul. 2018
  (株)インターズー, Japanese
• 慢性僧帽弁疾患犬において右室strainによる右心室機能評価は優れた予後指標となり得るか
  森田智也; 中村健介; 大菅辰幸; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, SA Medicine, 20, 3, 68, 70, Jun. 2018
  (株)インターズー, Japanese
• 肺塞栓症において認められる心室中隔の奇異性運動は左右心室間同期障害によって生じる
  中村健介; 中村健介; 森田智也; 大菅辰幸; 池田正悟; DERMLIM Angkhana; 滝口満喜, 日本獣医麻酔外科学雑誌, 49, Suppl.1, 301, 301, Jun. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• 【症候シリーズ(Vol.20)発熱(1)】 感染性心内膜炎
  中村 健介, SA Medicine, 20, 2, 22, 25, Apr. 2018
  (株)インターズー, Japanese
• 犬と猫の膵炎~病態・診断・治療とモニタリング~膵炎の診断
  中村健介, CAP (Companion Animal Practice), 33, 2, 16, 23, Feb. 2018
  (株)緑書房, Japanese
• ピモベンダン投与による心機能指標の改善を心エコー図検査で捉えることができた慢性僧帽弁疾患の犬の1例
  中村健介, CAP (Companion Animal Practice), 33, 1, 91, 94, Jan. 2018
  (株)緑書房, Japanese
• JCVIM 2018 臨床研究アワード 慢性僧帽弁疾患犬において右室strainによる右心室機能評価は優れた予後指標となり得るか (第14回日本獣医内科学アカデミー学術大会(JCVIM 2018) 〈臨床研究アワード〉日本獣医内科学アカデミー/インターズー学術奨励賞受賞作品ダイジェスト版)
  森田 智也; 中村 健介; 大菅 辰幸; 森下 啓太郎; 佐々木 東; 大田 寛; 滝口 満喜, SA medicine : Journal of small animal medicine, 24, 3, 68, 70, 2018
  インターズー, Japanese
• 宮崎大学における大口径16列ヘリカルCT装置の画質向上に関する検討
  水谷真也; 水谷祐子; 佐藤裕之; 中村健介; 井上賀之; 金子泰之; 山本集士; ONG Benedict Huai Ern; 日高勇一; 永延清和; 鳥巣至道, 日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州), 2018, 2018
• 正常犬のMRI拡散テンソル画像パラメータ分析
  平山瑞紀; 水谷祐子; 水谷真也; 中村健介; 佐藤裕之; 浅沼武敏, 日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州), 2018, 2018
• 【犬の僧帽弁閉鎖不全症】僧帽弁閉鎖不全症の診断
  中村 健介, J-VET, 31, 1, 14, 24, Jan. 2018
  (株)インターズー, Japanese
• 周術期における肺血栓塞栓症が疑われ,t‐PA製剤による血栓溶解療法を行った犬の3例
  山本集士; 鳥巣至道; 金子泰之; 水谷真也; ONG Benedict; Huai Ern; 藤本晋輔; 藤本晋輔; 中村健介; 永延清和, 日本獣医麻酔外科学雑誌, 48, Supplement 2, 198, 20 Nov. 2017
  Japanese
• 心エコーで右心房を評価する
  中村健介, 日本獣医麻酔外科学雑誌, 48, Supplement 2, 29, 20 Nov. 2017
  Japanese
• タンパク喪失性腸症の治療最前線 1.病態と診断―治療の前にプローブを持ちながら考えるべきこと―
  中村健介, 動物臨床医学会年次大会プロシーディング, 38th, 1, 95‐96, 17 Nov. 2017
  Japanese
• 消化管系なっとく診断 2018 Part 3 エコー検査
  中村健介, ARCH, 7, 68, 78, Oct. 2017
  (株)ファームプレス, Japanese
• 「原因特定」だけじゃない,消化管エコーの+α
  中村健介, 日本臨床獣医学フォーラム年次大会プロシーディング, 19th, Vol.1, 078‐081, 15 Sep. 2017
  Japanese
• 脾静脈から副腎を横行する前腹静脈に短絡していた先天性門脈体循環シャントの犬の1例
  水谷 真也; 水谷 祐子; 中村 健介; 金子 泰之; 山本 集士; 鳥巣 至道, 日本獣医画像診断学会抄録, 62回, 7, 7, Sep. 2017
  日本獣医画像診断学会, Japanese
• ここまでできる!呼吸器疾患の画像診断 精密検査としての呼吸器エコー
  中村 健介, 日本獣医画像診断学会抄録, 62回, 14, 15, Sep. 2017
  日本獣医画像診断学会, Japanese
• 副腎偶発腫から診断した犬のサブクリニカルクッシング症候群の臨床的特徴
  永田矩之; 大田寛; 佐々木東; 森下啓太郎; 中村健介; 中村康介; 細谷謙次; 高木哲; 滝口満喜, 北海道獣医師会雑誌, 61, 8, 326, 326, Aug. 2017
  (公社)北海道獣医師会, Japanese
• 造影超音波検査により計測する肺通過時間は肺動脈塞栓モデル犬において短縮する
  中村 健介; 森田 智也; 大菅 辰幸; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 160回, 465, 465, Aug. 2017
  (公社)日本獣医学会, Japanese
• 慢性肺動脈塞栓性肺高血圧症モデル犬を用いたピモベンダンの作用に関する検討
  森田 智也; 中村 健介; 大菅 辰幸; 森下 啓太郎; 佐々木 東; 大田 寛; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 160回, 465, 465, Aug. 2017
  (公社)日本獣医学会, Japanese
• 超音波と膀胱内注入療法を組み合わせた膀胱癌治療法の開発
  佐々木東; 中山翔太; 森下啓太郎; 中村健介; 大田寛; 水川葉月; 池中良徳; 石塚真由美; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160回, 461, 461, Aug. 2017
  (公社)日本獣医学会, Japanese
• 正常犬ならびに慢性僧帽弁疾患犬におけるピモベンダン投与による左心房機能の変化
  三木伸悟; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 48, Suppl.1, 348, 348, Jun. 2017
  (一社)日本獣医麻酔外科学会, Japanese
• 急性圧負荷が右室にもたらす影響:心エコー図検査は何を反映しているか
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 48, Suppl.1, 336, 336, Jun. 2017
  (一社)日本獣医麻酔外科学会, Japanese
• 先天性門脈体循環シャントに伴う肝肺症候群および門脈肺高血圧症を疑った1例
  道下明日香; 中村健介; 森下啓太郎; 佐々木東; 大田寛; 細谷謙次; 賀川由美子; 滝口満喜, 日本獣医麻酔外科学雑誌, 48, Suppl.1, 220, 220, Jun. 2017
  (一社)日本獣医麻酔外科学会, Japanese
• 低強度パルス超音波による毛包構成細胞への刺激
  佐々木東; 工藤信樹; 中村健介; 森下啓太郎; 大田寛; 滝口満喜, 再生医療, 16, 348, 01 Feb. 2017
  Japanese
• ネオスポラ症を疑う犬の1例
  水谷祐子; 中村健介; 水谷真也; 浅沼武敏; 佐藤裕之, 日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州), 2017, 2017
• 症候シリーズ Vol.12 運動不耐性 猫の分類不能心筋症
  中村健介, SA Medicine, 18, 6, 42‐45, Dec. 2016, [Invited]
  Japanese, Introduction commerce magazine
• Post‐capillary肺高血圧症ではPre‐capillary肺高血圧症ほど右室機能指標は減弱しない
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Suppl.2, 228, 228, Nov. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• 部位別の基本描出シリーズ 腹部編 1)肝臓 X線検査
  中村健介, 伴侶動物画像診断, 1, 1, 38‐50, Oct. 2016, [Invited]
  Japanese, Introduction commerce magazine
• 部位別の基本描出シリーズ 腹部編 1)肝臓 超音波検査
  中村健介, 伴侶動物画像診断, 1, 1, 51‐73, Oct. 2016, [Invited]
  Japanese, Introduction commerce magazine
• 心エコー図検査にて急性期および回復後の変化を捉えた急性肺血栓塞栓症の犬の1症例
  森田智也; 中村健介; 森下啓太郎; 竹内恭介; 大菅辰幸; 那須香菜子; 華園究; 滝口満喜, 北海道獣医師会雑誌, 60, 8, 417, 417, 10 Aug. 2016
  (公社)北海道獣医師会, Japanese
• 好塩基球性白血病の犬の1症例
  渡邉清正; 森下啓太郎; 中村健介; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 60, 8, 406, 406, Aug. 2016
  (公社)北海道獣医師会, Japanese
• 大腸血管拡張症の犬の1症例
  竹内恭介; 大田寛; 高木哲; 中島彩乃; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 60, 8, 418, 418, Aug. 2016
  (公社)北海道獣医師会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープにおけるCOX‐2発現とToll‐like receptorとの関連性の解析
  長谷川純也; 大田寛; 横山望; 笹岡一慶; NISA Khoirun; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 日本獣医学会学術集会講演要旨集, 159回, 456, 456, Aug. 2016
  (公社)日本獣医学会, Japanese
• 犬の免疫介在性関節炎の疫学的および臨床的特徴
  永田 矩之; 中村 健介; 森下 啓太郎; 越後 良介; 奥村 正裕; 佐々木 東; 大田 寛; 滝口 満喜, 北海道獣医師会雑誌, 60, 8, 416, 416, Aug. 2016
  (公社)北海道獣医師会, Japanese
• 心筋症猫の心不全予測のために心エコー図検査にバイオマーカーを加える意義はあるか?
  中村健介; 堀泰智; 大菅辰幸; 森田智也, 日本獣医麻酔外科学雑誌, 47, Supplement 1, 324, 05 Jun. 2016
  Japanese
• Parametric Micro flow imaging法を用いて肝実質の動脈化を検出した門脈血栓症の犬の1例
  田村昌大; 中村健介; 森俊輔; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Suppl.1, 335, 335, Jun. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• 間質性肺疾患による重度肺高血圧症を呈したウェルシュ・コーギーの2例
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Suppl.1, 235, 235, Jun. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• 造影超音波検査により計測した肺循環時間と慢性房室弁疾患の重症度との関係
  池田正悟; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Suppl.1, 323, 323, Jun. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の膵炎診断における客観的で簡便な指標による超音波検査の有用性の検討
  竹内 恭介; 中村 健介; 森下 啓太郎; 大田 寛; 佐々木 東; 滝口 満喜, 日本獣医師会雑誌, 69, 5, 292, 292, May 2016
  (公社)日本獣医師会, Japanese
• 右心機能の低下は僧帽弁疾患犬の生存期間を短縮する
  森田 智也; 中村 健介; 大菅 辰幸; 佐々木 東; 大田 寛; 滝口 満喜, 日本獣医師会雑誌, 69, 5, 294, 294, May 2016
  (公社)日本獣医師会, Japanese
• 心タンポナーデ 2「心タンポナーデ」の診断
  中村健介, Veterinary Circulation, 5, 2, 11‐18, 18, May 2016, [Invited]
  インターズー, Japanese, Introduction commerce magazine
• シスプララチンデリバリーの効果が及ぶ距離
  佐々木 東; 工藤 信樹; 中村 健介; 森下 啓太郎; 大田 寛; 滝口 満喜, 超音波医学, 43, Suppl., S544, S544, Apr. 2016
  (公社)日本超音波医学会, Japanese
• 日本の専門家が結集して作る獣医臨床腫瘍学テキストブック 各論 消化器系(食道~結腸・直腸,肛門)画像診断
  中村健介, Veterinary Oncology, 3, 2, 85‐93, 84, Apr. 2016, [Invited]
  (株)エデュワードプレス, Japanese, Introduction commerce magazine
• 症候シリーズ Vol.7 発咳 肺炎
  中村健介, SA Medicine, 18, 1, 22, 25, Feb. 2016, [Invited]
  Japanese, Introduction commerce magazine
• Unclassifiable cardiomyopathy of cats
  中村 健介, SA medicine : Journal of small animal medicine, 22, 6, 42, 45, 2016
  インターズー, Japanese
• 活性型ビタミンD₃外用剤の使用中に飼い犬に腎不全をきたした尋常性乾癬の1例
  藤田靖幸; 華園究; 中村健介; 内田英二; 川畑智敬; 須藤明日香; 清水宏, 日本乾癬学会学術大会プログラム・抄録集, 31st, 157, 2016
  Japanese
• 猫の動的左室流出路閉塞に観血的動脈血圧測定を用いて全身麻酔管理を行った1例
  伊丹貴晴; 高木哲; 武内亮; 星清貴; 華園究; 中村健介; 奥村正裕, 日本獣医麻酔外科学雑誌, 47, 1, 13‐19(J‐STAGE), 19, 2016
  (一社)日本獣医麻酔外科学会, Japanese
• 特集「犬の呼吸器症状の治療ー末梢気道および肺実質の疾患ー」末梢気道および肺実質疾患の治療②誤嚥性肺炎
  中村 健介, CLINIC NOTE, 12, 1, 56, 61, Jan. 2016, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• 【猫の肥大型心筋症と大動脈血栓塞栓症】 肥大型心筋症の病態生理と診断
  中村 健介, J-VET, 29, 1, 8, 19, Jan. 2016, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• 犬の呼吸器症状の治療―末梢気道および肺実質の疾患 Case Report 診断および治療の要点 末梢気道および肺実質疾患の治療 2)
  NAKAMURA KENSUKE, CLINIC NOTE, 12, 1, 48, 55, Jan. 2016, [Invited]
  インターズー, Japanese, Introduction commerce magazine
• 猫の左室流出路動的狭窄に観血的動脈血圧測定を用いて全身麻酔管理を行った1例
  伊丹貴晴; 高木哲; 中村健介; 華園究; 武内亮; 星清貴; 奥村正裕, 日本獣医麻酔外科学雑誌, 46, Supplement 2, 225, 225, 05 Dec. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 右心室‐左心室‐左心房interaction:犬急性肺高血圧症モデルにおける検討
  大菅辰幸; 中村健介; 森田智也; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Suppl.2, 251, 251, Dec. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 診察中に左房破裂による心タンポナーデを発症したものの内科療法のみで長期生存中の犬の1例
  川元誠; 中村健介; 高瀬奈美; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Suppl.2, 241, 241, Dec. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 猫の尿管結石・閉塞23例における内科療法の反応と外科手術法の検討
  星 清貴; 高木 哲; 細谷 謙次; 星野 有希; 中村 健介; 森下 啓太郎; 金 尚昊; 奥村 正裕, 日本獣医麻酔外科学雑誌, 46, Suppl.2, 227, 227, Dec. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 胆管原発カルチノイドによる肝外胆管閉塞と診断した猫の1例
  森俊輔; 中村健介; 高木哲; 賀川由美子; 森下啓太郎; 大田寛; 滝口満喜, 動物臨床医学会年次大会プロシーディング, 36回, 2, 283, 284, Nov. 2015
  動物臨床医学会, Japanese
• 犬の非再生性免疫介在性貧血(NRIMA)に対する治療の前向き検討
  森下啓太郎; 竹内恭介; 森俊輔; 早川小百合; 中村健介; 佐々木東; 大田寛; 滝口満喜, 動物臨床医学会年次大会プロシーディング, 36回, 2, 367, 368, Nov. 2015
  動物臨床医学会, Japanese
• 犬のびまん性肝疾患に対する造影超音波検査の応用の試み
  道下 明日香; 森下 啓太郎; 華園 究; 中村 健介; 滝口 満喜; 高木 哲, 日本獣医画像診断学会抄録, 58回, 12, 12, Sep. 2015
  (一社)日本獣医画像診断学会, Japanese
• 汎用性が高く臨床的にも有用な犬の左心房機能評価法の検討;僧帽弁逆流の重症度評価について
  川元誠; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 390, 390, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 右心機能の低下は僧帽弁疾患犬の生存期間を短縮する
  森田智也; 中村健介; 大菅辰幸; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 389, 389, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 超低脂肪食で治療した犬慢性腸症18例の回顧的検討
  横山望; 大田寛; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 394, 394, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 犬の膵炎診断における客観的で簡便な指標による超音波検査の有用性の検討
  竹内恭介; 中村健介; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 396, 396, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 心エコー図検査による右心機能指標と心臓カテーテル検査との関連:急性肺高血圧モデル犬を用いた検討
  森田智也; 中村健介; 大菅辰幸; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 日本獣医学会学術集会講演要旨集, 158回, 403, 403, Aug. 2015
  (公社)日本獣医学会, Japanese
• 免疫抑制治療中に合併したRgizopus microsporus感染に伴う胃穿孔の犬の1例
  那須 香菜子; 中村 健介; 高木 哲; 賀川 由美子; 青島 圭佑; 甲斐 千暁; 後藤 正光; 滝口 満喜, 北海道獣医師会雑誌, 59, 8, 392, 392, Aug. 2015
  (公社)北海道獣医師会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるToll‐like receptor2,4の局在解析
  横山望; 大田寛; 賀川由美子; 山崎淳平; 市居修; KHOIRUN Nisa; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 日本獣医学会学術集会講演要旨集, 158回, 402, 402, Aug. 2015
  (公社)日本獣医学会, Japanese
• 胸部のX線画像―症例からみる胸部の画像診断―第16回 突然の呼吸困難と食欲廃絶を示した犬の1例
  NAKAMURA KENSUKE, CLINIC NOTE, 11, 7, 41, 51, Jul. 2015, [Invited]
  インターズー, Japanese, Introduction commerce magazine
• 鼻腔内に発生した歯原性嚢胞に対して外科治療を実施した短頭種犬の2症例
  武内亮; 高木哲; 大田寛; 中村健介; 伊丹貴晴; 華園究; 細谷謙次; 賀川由美子; 奥村正裕, 日本獣医麻酔外科学雑誌, 46, Supplement 1, 242, 242, 10 Jun. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• Dynamic CTによる肝細胞性腫瘤の良悪性鑑別
  那須香菜子; 中村健介; 高木哲; 細谷謙次; 華園究; 賀川由美子; 森下啓太郎; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Supplement 1, 302, 302, 10 Jun. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 右室Tei‐indexは僧帽弁疾患犬の1年生存を規定する
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Suppl.1, 280, 280, Jun. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 僧帽弁疾患犬におけるうっ血性心不全の発症はLA strain法によるブースターポンプ機能指標と関連する
  川元誠; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Suppl.1, 281, 281, Jun. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 【一目でわかる症候シリーズVol.3 心雑音・チアノーゼ】 心房中隔欠損症
  中村 健介, SA Medicine, 17, 3, 34, 37, Jun. 2015, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• 嘔吐を主訴とする症例における超音波検査の使い方
  NAKAMURA KENSUKE, CAP (Companion Animal Practice), 312, 95, 99, Jun. 2015, [Invited]
  Japanese, Introduction commerce magazine
• 肝静脈造影超音波検査による肝疾患罹患犬の肝内血行動態の評価
  森下 啓太郎; 平本 彰; 大菅 辰幸; 中村 健介; 大田 寛; 滝口 満喜, 日本獣医師会雑誌, 68, 5, 326, 327, May 2015
  (公社)日本獣医師会, Japanese
• 脾臓の腫瘤のアプローチ法と治療 part02 脾臓腫瘤の画像診断
  NAKAMURA KENSUKE, Veterinary Oncology, 2, 2, 12, 21, Apr. 2015, [Invited]
  Japanese, Introduction commerce magazine
• 動物循環器疾患研究の最前線 心エコー図検査による左心房機能解析の妥当性と有用性
  NAKAMURA KENSUKE, 獣医畜産新報, 68, 1124, 175, 178, Mar. 2015, [Invited]
  文永堂出版, Japanese, Introduction commerce magazine
• 肝静脈造影超音波検査による肝疾患罹患犬の肝内血行動態の評価
  森下啓太郎; 平本彰; 大菅辰幸; 中村健介; 大田寛; 滝口満喜, 日本獣医師会獣医学術学会年次大会講演要旨集, 2014, 137, 30 Jan. 2015
  Japanese
• 【犬と猫の膵炎〜今知っておくべきこと】 新しい画像検査 膵臓の造影超音波検査
  中村 健介, J-VET, 28, 1, 30, 35, Jan. 2015, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• 犬におけるSpeckle tracking法を用いた右室strain解析の再現性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.2, 216, 216, Dec. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• うっ血性左心不全を呈した原発性心臓血管肉腫の犬の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; TOOYAMA NOBUO; MORITA TOMOYA; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 33, 34, Nov. 2014
  動物臨床医学会, Japanese
• 免疫抑制量のプレドニゾロンが奏功した重度肺高血圧症を伴う間質性肺疾患を疑ったウェルシュコーギーの1例
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 103, 104, Nov. 2014
  動物臨床医学会, Japanese
• NSAIDsが原因と考えられた骨髄壊死の犬の1例
  MORISHITA KEITARO; DEGUCHI TATSUYA; KAGAWA YUMIKO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 93, 94, Nov. 2014
  動物臨床医学会, Japanese
• 犬慢性消化器疾患における十二指腸造影超音波検査の有用性の検討
  篠原 正義; リン・スーイー; 森下 啓太郎; 横山 望; 大菅 辰幸; 中村 健介; 賀川 由美子; 大田 寛; 滝口 満喜, 日本獣医画像診断学会抄録, 56回, 24, 24, Sep. 2014
  日本獣医画像診断学会, Japanese
• 【肝臓腫瘍】 肝臓超音波検査・ソナゾイド
  中村 健介, Joncol: Japanese Journal of Veterinary Clinical Oncology, 10, 2, 24, 28, Sep. 2014, [Invited]
  (株)ファームプレス, Japanese, Introduction commerce magazine
• 犬における全腹部照射(Whole Abdominal Irradiation:WAI)プロトコールの検討
  出口 辰弥; 細谷 謙次; 高木 哲; 星野 有希; 中村 健介; 金 尚昊; 奥村 正裕, 北海道獣医師会雑誌, 58, 8, 362, 362, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 4歳齢の毛球の食道内塞栓により発見された右大動脈弓遺残の猫の1例
  村上 翔輝; 高木 哲; 中村 健介; 岩木 芳美; 金 尚昊; 星野 有希; 細谷 謙次; 奥村 正裕, 北海道獣医師会雑誌, 58, 8, 373, 373, Aug. 2014
  (公社)北海道獣医師会, Japanese
• プロカルバジンが奏功した肉芽腫性髄膜脳脊髄炎を疑う犬の1例
  YAMAGUCHI TOMOO; OTA HIROSHI; MATSUMOTO MASANORI; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 365, 365, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 肺高血圧症の犬に対するDual pulsed‐wave Doppler法を用いた右室Tei‐index測定の有用性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 369, 369, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 尿管結石による水腎症から膿腎症に至った犬の1例
  HAYAKAWA SAYURI; NAKAMURA KENSUKE; TAKAGI SATOSHI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 376, 376, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 二次的な巨大食道を伴った鼻咽頭ポリープの猫の1例
  NASU KANAKO; NAKAMURA KENSUKE; TAKAGI SATOSHI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 374, 374, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 活性型ビタミンD₃外用薬の慢性摂取により高カルシウム血症が認められた犬の2例
  SHIMOIDE ARISA; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 379, 379, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 肝静脈造影超音波検査による肝疾患罹患犬の肝内血行動態の評価
  MORISHITA KEITARO; HIRAMOTO AKIRA; RIN SU・I; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 370, 370, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 左心房機能は心血管薬によりいかに変化するか:正常犬における2D Speckle Tracking法を用いた検討
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORITA TOMOYA; YEE LIM SUE; YOKOYAMA NOZOMU; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 157回, 479, 479, Aug. 2014
  (公社)日本獣医学会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるclaudinの蛋白質発現解析
  YOKOYAMA NOZOMU; OTA HIROSHI; MORITA TOMOYA; OSUGA TATSUYUKI; LIM SUE YEE; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 157回, 491, 491, Aug. 2014
  (公社)日本獣医学会, Japanese
• 胸水 Chapter 1 貯留のメカニズムと抜去テク
  NAKAMURA KENSUKE, Vet Circ, 3, 3, 6, 12, Aug. 2014, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• 犬の非感染性髄膜脳炎症例の脳脊髄液中におけるサイトカインの発現解析
  OTA HIROSHI; KAMADA MAYUMI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医神経病学会, 40th, 19, 05 Jul. 2014
  Japanese
• 犬胃癌10症例における超音波検査所見
  YOKOYAMA NOZOMI; OTA HIROSHI; NAKAMURA KENSUKE; MORISHITA KEITARO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 261, 261, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 造影超音波検査による腎機能評価の基礎的検討
  MORISHITA KEITARO; RIN SU・I; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 262, 262, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 徐脈性不整脈に対してピモベンダンを投与し失神の改善を認めた犬の1例
  MORITA TOMOYA; SUZUKI SHUJI; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 248, 248, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 2D Speckle Tracking法を用いた左心房機能評価による犬心筋症の重症度評価
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; SUZUKI SHUJI; MORISHITA KEITARO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 67, 5, 367, 367, 20 May 2014
  (公社)日本獣医師会, Japanese
• プレドニゾロンを主とする免疫抑制療法実施症例における犬膵特異的リパーゼの推移
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 67, 5, 369, 369, May 2014
  (公社)日本獣医師会, Japanese
• セルレイン誘発膵炎犬における造影超音波検査の定量的および定性的評価
  LIM SUE YEE; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医畜産新報, 67, 4, 286, 288, Apr. 2014, [Invited]
  文永堂出版(株), Japanese
• Dual pulsed‐wave doppler法による右室Tei‐index測定の再現性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; SUZUKI SHUJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 44, Suppl.2, 250, 250, Dec. 2013
  (一社)日本獣医麻酔外科学会, Japanese
• 猫心筋症において2D Speckle Tracking法を用いた左心房機能評価は可能であるのか
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; SUZUKI SHUJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 44, Suppl.2, 249, 249, Dec. 2013
  (一社)日本獣医麻酔外科学会, Japanese
• 両側性尿管閉塞を呈した犬の1例
  TOOYAMA NOBUO; NAKAMURA KENSUKE; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; TAKAGI SATOSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 3, 4, Nov. 2013
  動物臨床医学会, Japanese
• 非典型的溶血性尿毒症症候群(D‐HUS)が疑われた犬の1例
  MORISHITA KEITARO; TOOYAMA NOBUO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 141, 142, Nov. 2013
  動物臨床医学会, Japanese
• 慢性特発性偽性腸閉塞症が疑われた犬の1例
  YOKOYAMA NOZOMU; OTA HIROSHI; HASHIBA KANO; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OCHIAI KENJI; TERAMOTO HIDESHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 59, 60, Nov. 2013
  動物臨床医学会, Japanese
• 市販フードの給餌によって病的骨折を起こした栄養性二次性上皮小体機能亢進症の犬の1例
  HASHIBA KANO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 119, 120, Nov. 2013
  動物臨床医学会, Japanese
• 組織奇形が疑われた膀胱粘膜下嚢胞の犬の1例
  MORISHITA KEITARO; HOSOYA KENJI; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 9, 10, Nov. 2013
  動物臨床医学会, Japanese
• 超音波検査により診断が可能であった子宮捻転の犬の1例
  中村 健介; 山崎 真大; 大田 寛; 森下 啓太郎; 木村 享史; 滝口 満喜, 日本獣医画像診断学会抄録, 54回, 28, 28, Sep. 2013
  日本獣医画像診断学会, Japanese
• プレドニゾロンを主とする免疫抑制療法実施症例における犬膵特異的リパーゼの推移
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 402, 402, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 2D Speckle Tracking法を用いた左心房機能評価による犬心筋症の重症度評価
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; HOSOYA KENJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 396, 396, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 非典型的溶血性尿毒症症候群(D‐HUS)が疑われた犬の1例
  TOOYAMA NOBUO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 395, 395, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 閉鎖式持続吸引ドレーンにて良好にコントロールできた膵膿瘍の犬の1例
  YAMAGUCHI TOMOO; MORISHITA KEITARO; HOSOYA KENJI; MUKAIDANI RYUSUKE; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 400, 400, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 胸部後大静脈内浸潤を伴う褐色細胞腫の犬の1症例
  足立 真実; 高木 哲; 中村 健介; 大菅 辰幸; 星野 有希; 細谷 謙次; 奥村 正裕, 北海道獣医師会雑誌, 57, 8, 393, 393, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 【咳-循環器科と呼吸器科のアプローチ-】 (Chapter4)循環器科の症例評価と治療
  中村 健介, Veterinary Circulation, 2, 3, 37, 50, Aug. 2013, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• 特発性収縮性心膜炎の犬の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; HOSOYA KENJI; MORITA TOMOYA; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 44, Suppl.1, 245, 245, Jun. 2013
  (一社)日本獣医麻酔外科学会, Japanese
• ―肝疾患の検査と診断 解釈のポイント―画像診断による肝臓の系統的アプローチ~X線・超音波検査の次に何をすべきか?~
  NAKAMURA KENSUKE, CAP (Companion Animal Practice), 288, 28, 36, Jun. 2013, [Invited]
  Japanese, Introduction commerce magazine
• MARKED INCREASED EXPRESSION OF INTERLEUKIN 8 IN THE COLORECTAL MUCOSA OF DOGS WITH INFLAMMATORY COLORECTAL POLYPS BUT NOT WITH LYMPHOCYTIC-PLASMACYTIC COLITIS
  Y. Tamura; H. Ohta; N. Yokoyama; K. Morishita; K. Nakamura; M. Yamasaki; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 27, 3, 706, 706, May 2013
  English, Summary international conference
• QUALITATIVE AND QUANTITATIVE CONTRAST ENHANCED ULTRASONOGRAPHIC ASSESSMENT OF CERULEIN-INDUCED PANCREATITIS IN DOGS
  S. Y. Lim; K. Nakamura; K. Morishita; H. Ohta; M. Yamasaki; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 27, 3, 707, 707, May 2013
  English, Summary international conference
• 肝静脈血流波形解析による右心不全の重症度評価
  NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 66, 5, 346, 347, May 2013
  (公社)日本獣医師会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープの病態
  OTA HIROSHI; TAMURA YU; YOKOYAMA NOZOMI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 155回, 153, 153, Mar. 2013
  (公社)日本獣医学会, Japanese
• 末梢肺動脈狭窄症と診断した高齢猫の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.2, 213, 213, Dec. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• Babesia gibsoniにおけるdiminazene aceturate耐性の性質の安定性
  B.K.・ウィクラマセカラ・ラジャパクシャゲ; 山崎 真大; S.J.・ファン; 中村 健介; 佐々木 東; 村上 正紘; 田村 悠; S.Y.・リン; 大田 寛; 滝口 満喜, 獣医寄生虫学会誌, 11, 1, 35, 35, Oct. 2012
  日本獣医寄生虫学会, Japanese
• ソナゾイド造影超音波検査と機械学習を組み合わせた脾臓腫瘤の自動良悪性鑑別の試み
  中村 健介; 滝口 満喜; 高木 一也; 近藤 敏志, 日本獣医画像診断学会抄録, 52回, 16, 16, Sep. 2012
  (一社)日本獣医画像診断学会, Japanese
• 腱索断裂を伴う僧帽弁閉鎖不全症の小型犬に僧帽弁形成術を施行した1例
  沢田保; 前谷茂樹; 玉井聡; 岸原圭一郎; 伊丹貴晴; 今道昭一; 中村健介; 水野祐; 原田佳代子; 内田周平; 上地正実, 北海道獣医師会雑誌, 56, 8, 409, 10 Aug. 2012
  Japanese
• 好酸球増多症候群を伴った消化器型リンパ腫の猫の1例
  TOOYAMA NOBUO; HOSOYA KENJI; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 399, 399, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 下垂体性副腎皮質機能亢進症とクロム親和性細胞腫を併発した犬の1例
  KANAZAWA TOMONORI; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 410, 410, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 麻酔後の全脳虚血が疑われた2症例のMRI所見
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 429, 429, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 肝静脈血流波形解析による右心不全の重症度評価
  NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 409, 409, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 両側性子宮分節性無形成から片側性子宮角捻転に至った犬の1例
  YOKOYAMA NOZOMI; NAKAMURA KENSUKE; KIMURA TAKASHI; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 418, 418, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 下垂体性副腎皮質機能亢進症と中枢性尿崩症を併発し,放射線治療を行った下垂体腫瘍の犬の1例
  田村悠; 山崎真大; 森下啓太郎; 中村健介; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 56, 8, 410, 410, Aug. 2012
  (公社)北海道獣医師会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ病変における炎症性サイトカインの発現解析
  田村悠; 大田寛; 村上正紘; BANDULA Kumara WR; SUE Yee Lim; 大菅辰幸; 森下啓太郎; 中村健介; 山崎真大; 滝口満喜, 日本獣医学会学術集会講演要旨集, 154回, 307, 307, Aug. 2012
  (公社)日本獣医学会, Japanese
• 犬の頭蓋内疾患診断における経頭蓋超音波ドプラ法の有用性の検討
  SASAOKA KAZUYOSHI; NAKAMURA KENSUKE; MORISHITA KEITARO; OSUGA TATSUYUKI; LIM SUE YEE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 270, 270, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• CT検査を実施した肝葉捻転の犬の2例
  MORISHITA KEITARO; HOSOYA KENJI; TAKAGI SATOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 267, 267, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• 房室ブロックの犬2例に認められた低速僧帽弁逆流と高速左室駆出血流
  NAKAMURA KENSUKE; TOOYAMA NOBUO; UENO TAKASHI; MORISHITA KEITARO; OSUGA TATSUYUKI; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 245, 245, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• LEFT ATRIAL FUNCTION ANALYSIS BY LEFT ATRIAL TRACKING METHOD IN CANINE CHRONIC MITRAL VALVULAR HEART DISEASE.
  K. Nakamura; T. Osuga; M. Yamasaki; H. Ohta; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 26, 3, 720, 720, May 2012
  English, Summary international conference
• INTERLEUKIN-17A GENE EXPRESSION IN THE INTESTINAL MUCOSA IN DOGS WITH LYMPHOCYTIC-PLASMACYTIC ENTERITIS AND INFLAMMATORY COLORECTAL POLYP
  H. Ohta; K. Takada; Y. Tamura; K. Nakamura; M. Yamasaki; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 26, 3, 772, 773, May 2012
  English, Summary international conference
• 診断装置によるソノポレーションの特徴と条件設定
  SASAKI NOBORU; NAKAMURA KENSUKE; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 39, 2, 226, 227, 15 Mar. 2012
  (公社)日本超音波医学会, Japanese
• ジミナゼン耐性株および野生株におけるBabesia gibsoniミトコンドリアDNAの塩基配列の比較
  ウイクラマセカラ B.K.; ラジャパク・シャゲ; 山崎 真大; 中村 健介; 佐々木 東; 村上 正紘; 田村 悠; リン S.Y.; 大田 寛; 滝口 満喜, 獣医寄生虫学会誌, 10, 1-2, 34, 34, Mar. 2012
  日本獣医寄生虫学会, Japanese
• Babesia gibsoniにおけるdiminazene aceturate抵抗性の安定性(Stability of characteristic of the diminazene aceturate resistance in Babesia gibsoni)
  Rajapakshage Bandula Kumara Wickramasekara; 山崎 真大; Hwang Shiang-Jyi; 中村 健介; 佐々木 東; 村上 正紘; 田村 悠; Lim Sue Yee; 大田 寛; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 153回, 221, 221, Mar. 2012
  (公社)日本獣医学会, English
• 心エコー図検査にて心内膜心筋線維症と診断した猫4例
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; MURAKAMI MASAHIRO; MATSUYAMA ARATA; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 42, Suppl.2, 233, 233, Dec. 2011
  (一社)日本獣医麻酔外科学会, Japanese
• 活性型ビタミンD₃外用薬(マキサカルシトール軟膏)常用患者の飼育犬に認められた高カルシウム血症
  TOOYAMA NOBUO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32nd, 2, 149, 150, 18 Nov. 2011
  動物臨床医学会, Japanese
• 小型犬における下垂体性副腎皮質機能亢進症の超音波診断基準
  MATSUYAMA ARATA; TOOYAMA NOBUO; MURAKAMI MASAHIRO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32nd, 3, 14, 18, 18 Nov. 2011
  動物臨床医学会, Japanese
• 超音波診断装置および超音波造影剤による抗がん剤の抗腫瘍効果の増強
  SASAKI NOBORU; NAKAMURA KENSUKE; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 38, 6, 674, 674, 15 Nov. 2011
  (公社)日本超音波医学会, Japanese
• 犬の肝臓腫瘤診断におけるソナゾイド造影超音波検査―4年間の検討―
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; HOSOYA KENJI; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32回, 3, 6, 9, Nov. 2011
  動物臨床医学会, Japanese
• ジミナゼンアセチュレート耐性Babesia gibsoni株と野生型におけるミトコンドリアDNA配列の比較(Comparison of the sequence of mitochondrial DNA from diminazene aceturate resistance Babesia gibsoni strain with those from wild type)
  Rajapakshage Bandula Kumara Wickramasekara; 山崎 真大; 中村 健介; 佐々木 東; 村上 正紘; 田村 悠; Lim Sue Yee; 大田 寛; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 152回, 203, 203, Aug. 2011
  (公社)日本獣医学会, English
• 犬の僧房弁閉鎖不全症におけるLeft atrial tracking法を用いた左心房機能評価の有用性
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 55, 8, 386, 386, Aug. 2011
  (公社)北海道獣医師会, Japanese
• 心エコー図検査とD‐dimer測定により診断し得た肺血栓塞栓症の犬の1例
  OGI NAOKO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 55, 8, 385, 385, Aug. 2011
  (公社)北海道獣医師会, Japanese
• 超音波診断装置および超音波造影剤による抗がん剤のソノポレーションの抗腫瘍効果
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; BANDULA KUMARA WR; LIM SUE YEE; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 152回, 302, 302, Aug. 2011
  (公社)日本獣医学会, Japanese
• 犬の慢性腸炎症例の腸粘膜におけるIL‐17A遺伝子の発現解析
  TAKADA KANAE; OTA HIROSHI; NAKAMURA KENSUKE; SASAKI NOBORU; MURAKAMI MASAHIRO; KUMARA W.R. BANDULA; TAMURA YU; LIM SUE YEE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 152回, 298, 298, Aug. 2011
  (公社)日本獣医学会, Japanese
• プロテオーム解析を用いた犬リポ蛋白質測定法の評価
  SUDO ASUKA; YAMASAKI MASAHIRO; NAKAMURA KENSUKE; SASAKI NOBORU; MURAKAMI MASAHIRO; KUMARA W.R. BANDULA; TAMURA YU; LIM SUE YEE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 152回, 291, 291, Aug. 2011
  (公社)日本獣医学会, Japanese
• 犬の慢性腸炎症例の結腸粘膜における細胞接着分子クローディンの発現変動
  OTA HIROSHI; TAMURA YU; LIM SUE YEE; KUMARA WR BANDULA; MURAKAMI MASAHIRO; SASAKI NOBORU; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 152回, 298, 298, Aug. 2011
  (公社)日本獣医学会, Japanese
• 気腫性胆嚢炎の犬の1例
  田村悠; 山崎真大; 中村健介; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 55, 8, 386, 386, Aug. 2011
  (公社)北海道獣医師会, Japanese
• 門脈体循環シャント(前編)門脈体循環シャントの画像診断:超音波検査
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE, Surgeon, 15, 4, 33, 38, Jul. 2011, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• 犬の心疾患におけるLeft atrial tracking法を用いた左心房機能評価の有用性
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 42, Suppl.1, 243, 243, Jun. 2011
  (一社)日本獣医麻酔外科学会, Japanese
• 仮想気管支鏡検査が診断に有用であった肺葉捻転の犬の2例
  MURAKAMI MASAHIRO; NAKAMURA KENSUKE; SASAKI NOBORU; MATSUYAMA SHIN; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 42, Suppl.1, 264, 264, Jun. 2011
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の肝カルチノイド4症例におけるソナゾイド造影超音波検査所見
  NAKAMURA KENSUKE; HOSOYA KENJI; YAMASAKI MASAHIRO; OTA HIROSHI; YEE LIM SUE; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 42, Suppl.1, 261, 261, Jun. 2011
  (一社)日本獣医麻酔外科学会, Japanese
• "診る目を養う"超音波検査(第6回) 嘔吐の原因を超音波で探る 胃の超音波検査
  滝口 満喜; 中村 健介, SA Medicine, 13, 3, 72, 78, Jun. 2011, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• ”診る目を養う”超音波検査 第6回 嘔吐の原因を超音波で探る(1)胃の超音波検査
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE, SA Med, 13, 3, 72, 78, Jun. 2011, [Invited]
  (株)エデュワードプレス, Japanese, Introduction commerce magazine
• EFFECTS OF SILDENAFIL CITRATE ON DOGS WITH EISENMENGER'S SYNDROME
  K. Nakamura; M. Yamasaki; H. Ohta; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 25, 3, 660, 660, May 2011
  English, Summary international conference
• パルス超音波と微小気泡によるシスプラチンの抗腫瘍効果増強の基礎的知見
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 38, 3, 338, 338, May 2011
  (公社)日本超音波医学会, Japanese
• 診断用超音波とマイクロバブルによるドラッグデリバリーのin vitroでの最適化
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BADULA; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 38, Suppl., S316, S316, Apr. 2011
  (公社)日本超音波医学会, Japanese
• “診る目を養う”超音波検査 第5回 門脈シャントを超音波で診断する
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, SA Med, 13, 2, 86, 90, Apr. 2011, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• シトシンアラビノシドにより良好に維持されている特発性脳炎の犬の2例
  OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医皮膚科学会学術大会・総会, 14th, Vol.1, 117, 11 Mar. 2011
  Japanese
• 二次性赤血球増加症を伴ったEisenmenger症候群の犬に対するシルデナフィルの有効性の検討
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医皮膚科学会学術大会・総会, 14th, Vol.1, 175, 11 Mar. 2011
  Japanese
• 超音波とマイクロバブルによるドラッグデリバリーに薬剤濃度とバブル濃度が及ぼす影響
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BANDULA; TAMURA YU; YEE LIM SUE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医皮膚科学会学術大会・総会, 14th, Vol.1, 183, 11 Mar. 2011
  Japanese
• 第二世代超音波造影剤ソナゾイドを用いた造影超音波検査による犬の肝臓および脾臓腫瘤の診断に関する研究
  NAKAMURA KENSUKE; TAKAGI SATOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; TAMURA YU; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 151回, 82, 82, Mar. 2011
  (公社)日本獣医学会, Japanese
• “診る目を養う”超音波検査 第4回 肝臓腫瘤を超音波で鑑別する
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, SA Med, 13, 1, 101, 107, Feb. 2011, [Invited]
  (株)インターズー, Japanese, Introduction commerce magazine
• “診る目を養う”超音波検査 第3回 黄疸を超音波で鑑別する(2)肝実質の超音波検査
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, SA Med, 12, 6, 65, 69, Dec. 2010, [Invited]
  (株)エデュワードプレス, Japanese, Introduction commerce magazine
• ガストリン産生性カルチノイドに対し徐放性オクトレオチド製剤を使用した犬の1例
  細谷 謙次; 金子 一成; 滝口 満善; 中村 健介; 高木 哲; 奥村 正裕, 動物臨床医学会年次大会プロシーディング, 31回, 2, 193, 194, Nov. 2010
  動物臨床医学会, Japanese
• “診る目を養う”超音波検査 第2回 黄疸を超音波で鑑別する(1)胆道系の超音波検査
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE, SA Med, 12, 5, 79, 85, Oct. 2010, [Invited]
  (株)エデュワードプレス, Japanese, Introduction commerce magazine
• 微小気泡存在下での診断用超音波照射によるシスプラチンの抗腫瘍効果増強の検討
  SASAKI AZUMA; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; BANDULA KUMARA; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 150回, 297, 297, Sep. 2010
  (公社)日本獣医学会, Japanese
• 犬のガストリノーマに対してサンドスタチンLARを投与した1例
  金子 一成; 細谷 謙次; 滝口 満善; 中村 健介; 北村 二郎; 高木 哲; 奥村 正裕, 北海道獣医師会雑誌, 54, 8, 450, 450, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 造影超音波検査により病変の特定が可能であったインスリノーマの犬2例
  NAKAMURA KENSUKE; TAKAGI SATOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 460, 460, Aug. 2010
  (公社)北海道獣医師会, Japanese
• リンパ管拡張症を疑う胸水貯留に続発して肺葉捻転を起こした犬の1例
  MURAKAMI MASAHIRO; NAKAMURA KENSUKE; SASAKI AZUMA; OTA HIROSHI; YAMASAKI MASAHIRO; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 464, 464, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 造影3DCTにより確定診断し得た犬の重複大動脈弓の1例
  MATSUYAMA SHIN; KANEKO KAZUNARI; YAMANE YOSHIHISA; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 458, 458, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 心房中隔欠損症と僧帽弁閉鎖不全症を併発した犬の1例
  OGI NAOKO; NAKAMURA KENSUKE; MATSUYAMA SHIN; HASHIBA MINORU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 458, 458, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 胆嚢粘液嚢腫の犬19症例の回顧的解析
  睦良田 尚充; 高木 哲; 細谷 謙次; 中村 健介; 大田 寛; 山崎 真大; 滝口 満喜; 佐藤 耕太; 奥村 正裕, 北海道獣医師会雑誌, 54, 8, 462, 462, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 二次性赤血球増加症を伴ったEisenmenger症候群の犬に対するシルデナフィルの有効性の検討
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 41, Suppl.1, 294, 294, Jul. 2010
  (一社)日本獣医麻酔外科学会, Japanese
• CONTRAST-ENHANCED ULTRASONOGRAPHY WITH SONAZOID (R) FOR CHARACTERIZATION OF FOCAL SPLENIC LESIONS
  K. Nakamura; H. Ohta; M. Yamasaki; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 24, 3, 733, 734, May 2010
  English, Summary international conference
• 超音波診断装置と微小気泡によるシスプラチンの抗腫瘍効果の増強
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BANDULA; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 37, Suppl., S322, S322, Apr. 2010
  (公社)日本超音波医学会, Japanese
• 犬の肝臓腫瘤30例に対するソナゾイド造影超音波検査
  NAKAMURA KENSUKE; TAKAGI SATOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; SASAKI NOBORU; TAKIGUCHI MITSUYOSHI, 日本獣医師会三学会年次大会講演要旨集, 2009, 237, 07 Jan. 2010
  Japanese
• 外科手術を実施した犬の肝細胞癌17症例の検討
  高木 哲; 滝口 満喜; 山崎 真大; 大田 寛; 中村 健介; 大崎 智弘; 細谷 謙次; 奥村 正裕, 獣医麻酔外科学雑誌, 40, Suppl.2, 196, 196, Dec. 2009
  (一社)日本獣医麻酔外科学会, Japanese
• 犬のリンパ球プラズマ細胞性腸炎における細胞接着分子クローディンの発現変動
  OTA HIROSHI; MURAKAMI MASAHIRO; KUMARA BANDURA; SASAKI NOBORU; NAKAMURA KENSUKE; KO KOJAKU; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 148回, 261, 261, Sep. 2009
  (公社)日本獣医学会, Japanese
• 犬の脾臓腫瘤におけるソナゾイド造影超音波検査の有用性
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 148回, 268, 268, Sep. 2009
  (公社)日本獣医学会, Japanese
• 犬の肝臓腫瘤28結節に対するソナゾイド造影超音波検査
  NAKAMURA KENSUKE; TAKAGI SATOSHI; SASAKI AKIRA; MURAKAMI MASAHIRO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 101, 101, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 筋炎の併発が疑われた劇症型重症筋無力症の犬の1例
  UKAI AYAKO; YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; NAKAO KIICHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 98, 98, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 本態性高Na血症が疑われた神経症状を示した犬と猫の2例
  MURAKAMI MASAHIRO; OTA HIROSHI; NAKAMURA KENSUKE; SASAKI AKIRA; YAMASAKI MASAHIRO; ETO MASAYUKI; KOSEKI YUMI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 97, 97, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 気管支内視鏡検査で気管支狭窄を認めた慢性発咳の犬の1症例
  SASAKI AKIRA; OTA HIROSHI; ANDO REMI; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 88, 88, Aug. 2009
  (公社)北海道獣医師会, Japanese
• ボストンテリアの脾臓捻転の1症例
  OTA HIROSHI; TAKAGI SATOSHI; NAKAZAWA SHIGEKI; MURAKAMI MASAHIRO; SASAKI AKIRA; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 89, 89, Aug. 2009
  (公社)北海道獣医師会, Japanese
• SLEが疑われた血球貪食症候群の猫の1例
  YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; SASAKI AZUMA; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会三学会年次大会講演要旨集, 2008, 296, 25 Dec. 2008
  Japanese
• 超音波検査により求めた正常なビーグル犬の甲状腺体積と体重の相関
  SASAKI NOBORU; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 29回, 3, 29, 32, Nov. 2008
  動物臨床医学会, Japanese
• 犬の肝臓における造影超音波検査の有用性の検討
  NAKAMURA KENSUKE; SASAKI HIGASHI; YOSHIKAWA MUNEYOSHI; O KOJAKU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 146回, 265, 265, Sep. 2008
  (公社)日本獣医学会, Japanese
• 犬の消化菅粘膜におけるクローディン分子群の発現:十二指腸を中心として
  YAMAGUCHI TOMOO; OTA HIROSHI; NAKAMURA KENSUKE; O KOJAKU; SASAKI HIGASHI; YOSHIKAWA MUNEYOSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 146回, 258, 258, Sep. 2008
  (公社)日本獣医学会, Japanese
• 抗リン脂質抗体症候群が疑われた不明熱のミニチュア・ダックスフンドの1例
  NAKAMURA KENSUKE; SASAKI NOBORU; YOSHIKAWA MUNEYOSHI; OTA HIROSHI; YAMAZAKI MASAHIRO; OBA MASAYO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 8, 410, 410, Aug. 2008
  (公社)北海道獣医師会, Japanese
• エリスロポエチン依存性の二次性多血症が疑われた腎リンパ腫の犬の1例
  SASAKI AZUMA; YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMAZAKI MASAHIRO; SATO KOTA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 8, 415, 415, Aug. 2008
  (公社)北海道獣医師会, Japanese
• SLEが疑われた血球貪食症候群の猫の1例
  YOSHIKAWA MUNEYOSHI; SHODA MASATO; NAKAMURA KENSUKE; SASAKI NOBORU; OTA HIROSHI; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 8, 410, 410, Aug. 2008
  (公社)北海道獣医師会, Japanese
• 第二世代超音波造影剤ソナゾイドによる肝臓腫瘤の診断
  NAKAMURA KENSUKE; OTA HIROSHI; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 39, Suppl.1, 227, 227, Jun. 2008
  (一社)日本獣医麻酔外科学会, Japanese
• 腹部エコー検査のススメ―小動物臨床における超音波造影剤の可能性
  NAKAMURA KENSUKE; TAKIGUCHI MAKI, CAP (Companion Anim Pract), 23, 225, 23, 28, Mar. 2008, [Invited]
  (株)緑書房, Japanese, Introduction commerce magazine
• クエン酸シルデナフィルが奏功した逆行性動脈管開存症のイヌの2例
  NAKAMURA KENSUKE; MORISHITA KEITARO; NAKADE TETSUYA; TAKIGUCHI MITSUYOSHI, 日本獣医師会三学会年次大会講演要旨集, 2007, 245, 2008
  Japanese
• 第二世代超音波造影剤ソナゾイドの小動物臨床への応用に関する基礎的検討
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE, 獣医麻酔外科学雑誌, 38, Supplement 2, 290, 291, 20 Nov. 2007
  Japanese
• 犬の膵疾患における超音波ヒストグラム解析の有用性に関する基礎的研究
  NAKAMURA KENSUKE; MORISHITA KEITARO; TAKIGUCHI MAKI, 日本獣医学会学術集会講演要旨集, 144th, 160, 160, 27 Aug. 2007
  (公社)日本獣医学会, Japanese
• クエン酸シルデナフィルが奏功した逆行性動脈管開存症の犬の2例
  NAKAMURA KENSUKE; MORISHITA KEITARO; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 51, 8, 333, 333, 01 Aug. 2007
  (公社)北海道獣医師会, Japanese
• メソトレキセートが奏功したステロイド抵抗性炎症性腸疾患の犬の1例
  MORISHITA KEITARO; NAKAMURA KENSUKE; HIRAYAMA KAZUKO; TANIYAMA HIROYUKI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 51, 8, 335, 335, 01 Aug. 2007
  (公社)北海道獣医師会, Japanese
• デジタルX線(DR)撮影下における非選択的血管造影法により逆行性動脈管開存症と診断された犬の2例
  MORISHITA KEITARO; NAKAMURA KENSUKE; NAKAIDE TETSUYA; TAKIGUCHI MAKI, 日本獣医循環器学会抄録, 86th, Suppl.1, 203, 203, 2007
  (一社)日本獣医麻酔外科学会, Japanese
• 十二指腸に閉塞性病変を伴った炎症性陽疾患の犬の1例
  中村 健介; 森下 啓太郎; 廉澤 剛; 平山 和子; 谷山 弘行; 滝口 満喜, 北海道獣医師会雑誌, 50, 8, 79, 79, Aug. 2006
  (公社)北海道獣医師会, Japanese
• I型偽性低アルドステロン症が疑われた犬の1例
  MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 336, 80, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• 犬の慢性リンパ性白血病:メルファラン継続投与による良好な長期コントロールの1例
  HIROSE TAKESHI; NAKAMURA KENSUKE; MORISHITA KEITARO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 346, 90, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• デジタルX線(DR)撮影下における非選択的血管造影法により逆行性動脈管開存症と診断された犬の1例
  TOOYAMA NOBUO; NAKAMURA KENSUKE; MORISHITA KEITARO; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 355, 99, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• 十二指腸に閉塞性病変を伴った炎症性腸疾患の犬の1例
  NAKAMURA KENSUKE; MORISHITA KEITARO; RENZAWA TSUYOSHI; HIRAYAMA KAZUKO; TANIYAMA HIROYUKI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 335, 01 Aug. 2006
  Japanese
• 肉芽腫性胃炎により幽門狭窄症を呈したイヌの一例
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 37, Supplement 1, 207, 207, 20 Jun. 2006
  Japanese
• 頻回の内視鏡下バルーン拡張術が功を奏した食道狭窄の猫の1例
  MIWA ERIKO; MIYATA KAYOKO; MACHIDA TATSUHIKO; NAKAMURA KENSUKE; NAKAIDE TETSUYA; TAKIGUCHI MAKI, 北海道獣医師会雑誌, 49, 8, 276, 60, 01 Aug. 2005
  (公社)北海道獣医師会, Japanese
• 超音波検査により粥状動脈硬化症と診断された犬の1例
  NAKAMURA KENSUKE; KITAMURA JIRO; TAKIGUCHI MAKI, 北海道獣医師会雑誌, 49, 8, 293, 77, 01 Aug. 2005
  (公社)北海道獣医師会, Japanese
• 血管超音波検査によりアテローム性動脈硬化症が疑われた犬の一例
  中村 健介; 北村 二郎; 滝口 満喜, 日本獣医循環器学会抄録, 82, 249, 249, 25 Jun. 2005
  Japanese
• 血管超音波検査によりアテローム性動脈硬化症が疑われた犬の一例
  NAKAMURA KENSUKE; KITAMURA JIRO; TAKIGUCHI MAKI, 獣医麻酔外科学雑誌, 36, Supplement 1, 240, 240, 20 Jun. 2005
  Japanese

• 腹部
  滝口, 満喜; 中村, 健介; 横山, 望
  学窓社, Mar. 2022, 9784873627823, 205p, Japanese
• 犬と猫の超音波 vol.1 心臓
  中村, 健介; 滝口, 満喜
  学窓社, Jun. 2020, 9784873627762, 182p, Japanese
• 僧帽弁逆流の犬における心エコーの使い方
  中村, 健介; 医療情報研究所
  医療情報研究所, 2019, ビデオディスク3枚 (135分), Japanese
• 犬と猫の治療ガイド2015 私はこうしている
  中村健介, 第２章 循環器疾患 猫の肥大型心筋症
  インターズー, Sep. 2015, [Joint work]
• 猫の心臓病 臨床とエビデンスにもとづく診断と治療
  中村健介, SECTION B 診断検査
  インターズー, Jul. 2015, [Joint translation]
• 犬と猫の超音波診断マニュアル
  中村健介, 第５章 胸部、第6章 心臓、第8章 肝臓、第9章 脾臓
  学窓社, Oct. 2013, [Joint translation]

• 心エコーで右心房を評価する
  中村健介
  日本獣医麻酔外科学雑誌, 20 Nov. 2017, Japanese
  [Invited]
• 周術期における肺血栓塞栓症が疑われ,t‐PA製剤による血栓溶解療法を行った犬の3例
  山本集士; 鳥巣至道; 金子泰之; 水谷真也; ONG Benedict; Huai Ern; 藤本晋輔; 藤本晋輔; 中村健介; 永延清和
  日本獣医麻酔外科学雑誌, 20 Nov. 2017, Japanese
• タンパク喪失性腸症の治療最前線 1.病態と診断―治療の前にプローブを持ちながら考えるべきこと―
  中村健介
  動物臨床医学会年次大会プロシーディング, 17 Nov. 2017, Japanese
  [Invited]
• 「原因特定」だけじゃない,消化管エコーの+α
  中村健介
  日本臨床獣医学フォーラム年次大会プロシーディング, 15 Sep. 2017, Japanese
• ここまでできる!呼吸器疾患の画像診断 精密検査としての呼吸器エコー
  中村健介
  日本獣医画像診断学会抄録(Web), Sep. 2017, Japanese
  [Invited]
• 脾静脈から副腎を横行する前腹静脈に短絡していた先天性門脈体循環シャントの犬の1例
  水谷真也; 水谷祐子; 中村健介; 金子泰之; 山本集士; 鳥巣至道
  日本獣医画像診断学会抄録(Web), Sep. 2017, Japanese
• 慢性肺動脈塞栓性肺高血圧症モデル犬を用いたピモベンダンの作用に関する検討
  森田智也; 中村健介; 大菅辰幸; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜
  日本獣医学会学術集会講演要旨集, 30 Aug. 2017, Japanese
• 造影超音波検査により計測する肺通過時間は肺動脈塞栓モデル犬において短縮する
  中村健介; 森田智也; 大菅辰幸; 滝口満喜
  日本獣医学会学術集会講演要旨集, 30 Aug. 2017, Japanese
• 超音波と膀胱内注入療法を組み合わせた膀胱癌治療法の開発
  佐々木東; 中山翔太; 森下啓太郎; 中村健介; 大田寛; 水川葉月; 池中良徳; 石塚真由美; 滝口満喜
  日本獣医学会学術集会講演要旨集, 30 Aug. 2017, Japanese
• 副腎偶発腫から診断した犬のサブクリニカルクッシング症候群の臨床的特徴
  永田 矩之; 大田 寛; 佐々木 東; 森下 啓太郎; 中村 健介; 細谷 謙次; 高木 哲; 滝口 満喜
  北海道獣医師会雑誌, Aug. 2017, Japanese
• 造影超音波検査の現状と未来
  中村健介
  日本獣医麻酔外科学雑誌, 05 Jun. 2017, Japanese
  [Invited]
• 正常犬ならびに慢性僧帽弁疾患犬におけるピモベンダン投与による左心房機能の変化
  三木 伸悟; 中村 健介; 大菅 辰幸; 森田 智也; 佐々木 東; 森下 啓太郎; 大田 寛; 滝口 満喜
  日本獣医麻酔外科学雑誌, 05 Jun. 2017, Japanese
• 急性圧負荷が右室にもたらす影響 心エコー図検査は何を反映しているか
  森田 智也; 中村 健介; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 大田 寛; 滝口 満喜
  日本獣医麻酔外科学雑誌, 05 Jun. 2017, Japanese
• 各種画像診断法における造影検査 造影超音波検査の現状と未来
  中村 健介
  日本獣医麻酔外科学雑誌, Jun. 2017, Japanese
• 先天性門脈体循環シャントに伴う肝肺症候群および門脈肺高血圧症を疑った1例
  道下 明日香; 中村 健介; 森下 啓太郎; 佐々木 東; 大田 寛; 細谷 謙次; 賀川 由美子; 滝口 満喜
  日本獣医麻酔外科学雑誌, Jun. 2017, Japanese
• 各種画像診断法における造影検査 造影超音波検査の現状と未来
  中村 健介
  日本獣医麻酔外科学雑誌, Jun. 2017, Japanese
  [Invited], [Domestic Conference]
• 正常犬ならびに慢性僧帽弁疾患犬におけるピモベンダン投与による左心房機能の変化
  三木 伸悟; 中村 健介; 大菅 辰幸; 森田 智也; 佐々木 東; 森下 啓太郎; 大田 寛; 滝口 満喜
  日本獣医麻酔外科学雑誌, Jun. 2017, Japanese
• 急性圧負荷が右室にもたらす影響 心エコー図検査は何を反映しているか
  森田 智也; 中村 健介; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 大田 寛; 滝口 満喜
  日本獣医麻酔外科学雑誌, Jun. 2017, Japanese
• 低強度パルス超音波による毛包構成細胞への刺激
  佐々木東; 工藤信樹; 中村健介; 森下啓太郎; 大田寛; 滝口満喜
  再生医療, 01 Feb. 2017, Japanese
• ネオスポラ症を疑う犬の1例
  水谷祐子; 中村健介; 水谷真也; 浅沼武敏; 佐藤裕之
  日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州), 2017, Japanese
• 圧負荷および容量負荷が右室strainに与える影響に関する実験的検討
  中村健介; 森田智也; 大菅辰幸; 滝口満喜
  日本心エコー図学会学術集会プログラム・抄録集(Web), 2017, Japanese
• Post‐capillary肺高血圧症ではPre‐capillary肺高血圧症ほど右室機能指標は減弱しない
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜
  日本獣医麻酔外科学雑誌, 20 Nov. 2016, Japanese
• 呼吸器疾患に関連した肺高血圧の治療
  中村健介
  日本獣医麻酔外科学雑誌, Nov. 2016, Japanese, Invited oral presentation
  [Invited]
• ミニチュア・ダックスフンドの炎症性結直腸ポリープにおけるCOX‐2発現とToll‐like receptorとの関連性の解析
  長谷川純也; 大田寛; 横山望; 笹岡一慶; NISA Khoirun; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜
  日本獣医学会学術集会講演要旨集, 30 Aug. 2016, Japanese
• 犬の免疫介在性関節炎の疫学的および臨床的特徴
  永田矩之; 中村健介; 森下啓太郎; 越後良介; 奥村正裕; 佐々木東; 大田寛; 滝口満喜
  北海道獣医師会雑誌, 10 Aug. 2016, Japanese
• 心エコー図検査にて急性期および回復後の変化を捉えた急性肺血栓塞栓症の犬の1症例
  森田智也; 中村健介; 森下啓太郎; 竹内恭介; 大菅辰幸; 那須香菜子; 華園究; 滝口満喜
  北海道獣医師会雑誌, 10 Aug. 2016, Japanese
• 大腸血管拡張症の犬の1症例
  竹内恭介; 大田寛; 高木哲; 中島彩乃; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜
  北海道獣医師会雑誌, 10 Aug. 2016, Japanese
• 好塩基球性白血病の犬の1症例
  渡邉清正; 森下啓太郎; 中村健介; 佐々木東; 大田寛; 滝口満喜
  北海道獣医師会雑誌, 10 Aug. 2016, Japanese
• 心筋症猫の心不全予測のために心エコー図検査にバイオマーカーを加える意義はあるか?
  中村健介; 堀泰智; 大菅辰幸; 森田智也
  日本獣医麻酔外科学雑誌, 05 Jun. 2016, Japanese
• 造影超音波検査により計測した肺循環時間と慢性房室弁疾患の重症度との関係
  池田正悟; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜
  日本獣医麻酔外科学雑誌, 05 Jun. 2016, Japanese
• 間質性肺疾患による重度肺高血圧症を呈したウェルシュ・コーギーの2例
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜
  日本獣医麻酔外科学雑誌, 05 Jun. 2016, Japanese
• Parametric Micro flow imaging法を用いて肝実質の動脈化を検出した門脈血栓症の犬の1例
  田村昌大; 中村健介; 森俊輔; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜
  日本獣医麻酔外科学雑誌, 05 Jun. 2016, Japanese
• シスプララチンデリバリーの効果が及ぶ距離
  佐々木東; 工藤信樹; 中村健介; 森下啓太郎; 大田寛; 滝口満喜
  超音波医学, 15 Apr. 2016, Japanese
• 日本小動物獣医学会シンポジウム「超音波定量診断技術の最前線」新しい心エコー図指標を用いた心不全の予後評価
  中村健介
  平成27年度日本獣医師会獣医学術学会年次大会, 27 Feb. 2016, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• 教育講演「心エコーで予後を評価する−犬の僧帽弁疾患の場合」
  中村健介
  第12回日本獣医内科学アカデミー学術大会, 20 Feb. 2016, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• 教育講演「エコーを活用した病態生理から考える腹水の鑑別診断」
  中村健介
  第12回日本獣医内科学アカデミー学術大会, 19 Feb. 2016, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• ランチョンセミナー「心エコーから考える薬の効き目」
  中村健介
  第103回日本獣医循環器学会, 19 Dec. 2015, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• 診察中に左房破裂による心タンポナーデを発症したものの内科療法のみで長期生存中の犬の1例
  KAWAMOTO MAKOTO; NAKAMURA KENSUKE; TAKASE NAMI; MORITA TOMOYA; OSUGA TATSUYUKI; SASAKI NOBORU; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  日本獣医麻酔外科学雑誌, 05 Dec. 2015, Japanese
• 猫の左室流出路動的狭窄に観血的動脈血圧測定を用いて全身麻酔管理を行った1例
  ITAMI TAKAHARU; TAKAGI SATOSHI; NAKAMURA KENSUKE; HANAZONO KIWAMU; TAKEUCHI RYO; HOSHI KIYOTAKA; OKUMURA MASAHIRO
  日本獣医麻酔外科学雑誌, 05 Dec. 2015, Japanese
• 右心室‐左心室‐左心房interaction:犬急性肺高血圧症モデルにおける検討
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORITA TOMOYA; MORISHITA KEITARO; SASAKI NOBORU; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  日本獣医麻酔外科学雑誌, 05 Dec. 2015, Japanese
• 猫の尿管結石・閉塞23例における内科療法の反応と外科手術法の検討
  HOSHI KIYOTAKA; TAKAGI SATOSHI; HOSOYA KENJI; HOSHINO YUKI; NAKAMURA KENSUKE; MORISHITA KEITARO; KIN SHOKO; OKUMURA MASAHIRO
  日本獣医麻酔外科学雑誌, 05 Dec. 2015, Japanese
• ランチョンセミナー「形態変化のその先へ−腹部エコーにおける血行評価−」
  中村健介
  第64回獣医学術九州地区学会, 16 Oct. 2015, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるToll‐like receptor2,4の局在解析
  YOKOYAMA NOZOMU; OTA HIROSHI; KAGAWA YUMIKO; YAMASAKI JUMPEI; ICHII OSAMU; KHOIRUN NISA; MORITA TOMOYA; OSUGA TATSUYUKI; SASAKI NOBORU; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 30 Aug. 2015, Japanese
• 心エコー図検査による右心機能指標と心臓カテーテル検査との関連:急性肺高血圧モデル犬を用いた検討
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; SASAKI NOBORU; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 30 Aug. 2015, Japanese
• 超低脂肪食で治療した犬慢性腸症18例の回顧的検討
  YOKOYAMA NOZOMU; OTA HIROSHI; SASAKI NOBORU; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2015, Japanese
• 右心機能の低下は僧帽弁疾患犬の生存期間を短縮する
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; SASAKI NOBORU; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2015, Japanese
• 犬の膵炎診断における客観的で簡便な指標による超音波検査の有用性の検討
  TAKEUCHI KYOSUKE; NAKAMURA KENSUKE; MORISHITA KEITARO; SASAKI NOBORU; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2015, Japanese
• 免疫抑制治療中に合併したRhizopus microsporus感染に伴う胃穿孔の犬の1例
  NASU KANAKO; NAKAMURA KENSUKE; TAKAGI SATOSHI; KAGAWA YUMIKO; AOSHIMA KEISUKE; KAI CHIAKI; GOTO MASAMITSU; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2015, Japanese
• 汎用性が高く臨床的にも有用な犬の左心房機能評価法の検討;僧帽弁逆流の重症度評価について
  KAWAMOTO MAKOTO; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORITA TOMOYA; SASAKI NOBORU; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2015, Japanese
• 僧帽弁疾患犬におけるうっ血性心不全の発症はLA strain法によるブースターポンプ機能指標と関連する
  KAWAMOTO MAKOTO; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORITA TOMOYA; SASAKI NOBORU; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  日本獣医麻酔外科学雑誌, 10 Jun. 2015, Japanese
• Dynamic CTによる肝細胞性腫瘤の良悪性鑑別
  NASU KANAKO; NAKAMURA KENSUKE; TAKAGI SATOSHI; HOSOTANI KENJI; HANAZONO KIWAMU; KAGAWA YUMIKO; MORISHITA KEITARO; TAKIGUCHI MITSUYOSHI
  日本獣医麻酔外科学雑誌, 10 Jun. 2015, Japanese
• 右室Tei‐indexは僧帽弁疾患犬の1年生存を規定する
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; SASAKI NOBORU; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  日本獣医麻酔外科学雑誌, 10 Jun. 2015, Japanese
• 鼻腔内に発生した歯原性嚢胞に対して外科治療を実施した短頭種犬の2症例
  TAKEUCHI RYO; TAKAGI SATOSHI; OTA HIROSHI; NAKAMURA KENSUKE; ITAMI TAKAHARU; HANAZONO KIWAMU; HOSOTANI KENJI; KAGAWA YUMIKO; OKUMURA MASAHIRO
  日本獣医麻酔外科学雑誌, 10 Jun. 2015, Japanese
• 教育講演「クイズで学ぶ胸部画像診断」
  中村健介
  第11回日本獣医内科学アカデミー学術大会, 22 Feb. 2015, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• パネルディスカッション「本音で語る犬と猫の膵炎」
  中村健介
  第11回日本獣医内科学アカデミー学術大会, 21 Feb. 2015, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• 犬におけるSpeckle tracking法を用いた右室strain解析の再現性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 05 Dec. 2014, Japanese
• ランチョンセミナー「心臓だけじゃもったいない！ドプラ法の真の実力」
  中村健介
  第35回動物臨床医学年次大会, 17 Nov. 2014, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• 心臓だけじゃもったいない!ドプラ法の真の実力
  NAKAMURA KENSUKE
  動物臨床医学会年次大会プロシーディング, 14 Nov. 2014, Japanese
• NSAIDsが原因と考えられた骨髄壊死の犬の1例
  MORISHITA KEITARO; DEGUCHI TATSUYA; KAGAWA YUMIKO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 14 Nov. 2014, Japanese
• 免疫抑制量のプレドニゾロンが奏功した重度肺高血圧症を伴う間質性肺疾患を疑ったウェルシュコーギーの1例
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 14 Nov. 2014, Japanese
• うっ血性左心不全を呈した原発性心臓血管肉腫の犬の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; TOOYAMA NOBUO; MORITA TOMOYA; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 14 Nov. 2014, Japanese
• 獣医師ランチョンセミナー「エビデンスに基づいた猫の心筋症および慢性腎疾患の治療方針」
  中村健介
  北海道小動物獣医師会年次大会2014, 02 Nov. 2014, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• シンポジウム「動物循環器研究の最前線」心エコー図検査による左心房機能解析の妥当性と有用性
  中村健介
  第157回日本獣医学会学術集会, 12 Sep. 2014, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• 市民公開講座「獣医のお仕事—動物と人の健康を守るために活躍する獣医さん−小動物臨床の現場から」
  中村健介
  第157回日本獣医学会学術集会・司宰機関企画市民公開講座, 06 Sep. 2014, English, Public discourse
  [Invited], [Domestic Conference]
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるclaudinの蛋白質発現解析
  YOKOYAMA NOZOMU; OTA HIROSHI; MORITA TOMOYA; OSUGA TATSUYUKI; LIM SUE YEE; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 11 Aug. 2014, Japanese
• 左心房機能は心血管薬によりいかに変化するか:正常犬における2D Speckle Tracking法を用いた検討
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORITA TOMOYA; YEE LIM SUE; YOKOYAMA NOZOMU; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 11 Aug. 2014, Japanese
• 肝静脈造影超音波検査による肝疾患罹患犬の肝内血行動態の評価
  MORISHITA KEITARO; HIRAMOTO AKIRA; RIN SU・I; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2014, Japanese
• 活性型ビタミンD₃外用薬の慢性摂取により高カルシウム血症が認められた犬の2例
  SHIMOIDE ARISA; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2014, Japanese
• 二次的な巨大食道を伴った鼻咽頭ポリープの猫の1例
  NASU KANAKO; NAKAMURA KENSUKE; TAKAGI SATOSHI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2014, Japanese
• 4歳齢の毛球の食道内塞栓により発見された右大動脈弓遺残の猫の1例
  MURAKAMI SHOKI; TAKAGI SATOSHI; NAKAMURA KENSUKE; IWAKI YOSHIMI; KIN SHOKO; HOSHINO YUKI; HOSOYA KENJI; OKUMURA MASAHIRO
  北海道獣医師会雑誌, 10 Aug. 2014, Japanese
• 尿管結石による水腎症から膿腎症に至った犬の1例
  HAYAKAWA SAYURI; NAKAMURA KENSUKE; TAKAGI SATOSHI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2014, Japanese
• 肺高血圧症の犬に対するDual pulsed‐wave Doppler法を用いた右室Tei‐index測定の有用性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2014, Japanese
• 犬における全腹部照射(Whole Abdominal Irradiation:WAI)プロトコールの検討
  DEGUCHI TATSUYA; HOSOYA KENJI; TAKAGI SATOSHI; HOSHINO YUKI; NAKAMURA KENSUKE; KIN SHOKO; OKUMURA MASAHIRO
  北海道獣医師会雑誌, 10 Aug. 2014, Japanese
• プロカルバジンが奏功した肉芽腫性髄膜脳脊髄炎を疑う犬の1例
  YAMAGUCHI TOMOO; OTA HIROSHI; MATSUMOTO MASANORI; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2014, Japanese
• 心エコー図検査による左心房機能解析の妥当性と有用性
  NAKAMURA KENSUKE
  日本獣医学会学術集会講演要旨集, Aug. 2014, Japanese, Oral presentation
  [Invited]
• 犬の非感染性髄膜脳炎症例の脳脊髄液中におけるサイトカインの発現解析
  OTA HIROSHI; KAMADA MAYUMI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医神経病学会, 05 Jul. 2014, Japanese
• 教育講演「X線検査でわかること、超音波検査でわかること」
  中村健介
  第55回日本獣医画像診断学会, 14 Jun. 2014, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• 徐脈性不整脈に対してピモベンダンを投与し失神の改善を認めた犬の1例
  MORITA TOMOYA; SUZUKI SHUJI; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 10 Jun. 2014, Japanese
• X線検査でわかること,超音波検査でわかること
  NAKAMURA KENSUKE
  獣医麻酔外科学雑誌, 10 Jun. 2014, Japanese
• 造影超音波検査による腎機能評価の基礎的検討
  MORISHITA KEITARO; RIN SU・I; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 10 Jun. 2014, Japanese
• 犬胃癌10症例における超音波検査所見
  YOKOYAMA NOZOMI; OTA HIROSHI; NAKAMURA KENSUKE; MORISHITA KEITARO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 10 Jun. 2014, Japanese
• プレドニゾロンを主とする免疫抑制療法実施症例における犬膵特異的リパーゼの推移
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医師会雑誌, 20 May 2014, Japanese
• 2D Speckle Tracking法を用いた左心房機能評価による犬心筋症の重症度評価
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; SUZUKI SHUJI; MORISHITA KEITARO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医師会雑誌, 20 May 2014, Japanese
• 教育講演「クイズで学ぶ胸部画像診断」
  中村健介
  日本獣医内科学アカデミー第10回記念学術大会, 09 Feb. 2014, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• ワークショップ「肺高血圧の診断・治療」
  中村健介
  日本獣医内科学アカデミー第10回記念学術大会, 07 Feb. 2014, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• シンポジウム肝臓腫瘍 肝臓超音波検査・ソナゾイド
  中村健介
  第10回日本獣医がん学会, 25 Jan. 2014, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• 猫心筋症において2D Speckle Tracking法を用いた左心房機能評価は可能であるのか
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; SUZUKI SHUJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 20 Dec. 2013, Japanese
• Dual pulsed‐wave doppler法による右室Tei‐index測定の再現性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; SUZUKI SHUJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 20 Dec. 2013, Japanese
• 組織奇形が疑われた膀胱粘膜下嚢胞の犬の1例
  MORISHITA KEITARO; HOSOYA KENJI; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 15 Nov. 2013, Japanese
• 市販フードの給餌によって病的骨折を起こした栄養性二次性上皮小体機能亢進症の犬の1例
  HASHIBA KANO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 15 Nov. 2013, Japanese
• 慢性特発性偽性腸閉塞症が疑われた犬の1例
  YOKOYAMA NOZOMU; OTA HIROSHI; HASHIBA KANO; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OCHIAI KENJI; TERAMOTO HIDESHI; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 15 Nov. 2013, Japanese
• 非典型的溶血性尿毒症症候群(D‐HUS)が疑われた犬の1例
  MORISHITA KEITARO; TOOYAMA NOBUO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 15 Nov. 2013, Japanese
• 両側性尿管閉塞を呈した犬の1例
  TOOYAMA NOBUO; NAKAMURA KENSUKE; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; TAKAGI SATOSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 15 Nov. 2013, Japanese
• 胸部後大静脈内浸潤を伴う褐色細胞腫の犬の1症例
  ADACHI MAMI; TAKAGI SATOSHI; NAKAMURA KENSUKE; OSUGA TATSUYUKI; HOSHINO YUKI; HOSOYA KENJI; OKUMURA MASAHIRO
  北海道獣医師会雑誌, 10 Aug. 2013, Japanese
• 閉鎖式持続吸引ドレーンにて良好にコントロールできた膵膿瘍の犬の1例
  YAMAGUCHI TOMOO; MORISHITA KEITARO; HOSOYA KENJI; MUKAIDANI RYUSUKE; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2013, Japanese
• 非典型的溶血性尿毒症症候群(D‐HUS)が疑われた犬の1例
  TOOYAMA NOBUO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2013, Japanese
• 2D Speckle Tracking法を用いた左心房機能評価による犬心筋症の重症度評価
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; HOSOYA KENJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2013, Japanese
• プレドニゾロンを主とする免疫抑制療法実施症例における犬膵特異的リパーゼの推移
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2013, Japanese
• 特発性収縮性心膜炎の犬の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; HOSOYA KENJI; MORITA TOMOYA; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 10 Jun. 2013, Japanese
• 肝静脈血流波形解析による右心不全の重症度評価
  NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医師会雑誌, 20 May 2013, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープの病態
  OTA HIROSHI; TAMURA YU; YOKOYAMA NOZOMI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 04 Mar. 2013, Japanese
• JCVIM循環器シンポジウム「犬の僧帽弁閉鎖不全症の重症度判定と外科療法」左心房機能に着目した心エコー図検査による新たな重症度判定法
  中村健介
  第9回日本獣医内科学アカデミー学術大会, 23 Feb. 2013, Nominated symposium
  [Invited], [Domestic Conference]
• 教育講演「肝腫瘤性病変の造影超音波検査」
  中村健介
  第9回日本獣医内科学アカデミー学術大会, 23 Feb. 2013, Invited oral presentation
  [Invited], [Domestic Conference]
• シンポジウム「肺高血圧症の治療を見直す：肺動脈性肺高血圧症の治療〜先天性・その他〜」
  中村健介
  第97回日本獣医循環器学会, 13 Jan. 2013, Nominated symposium
  [Invited], [Domestic Conference]
• 末梢肺動脈狭窄症と診断した高齢猫の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 10 Dec. 2012, Japanese
• 肺動脈性肺高血圧の治療~先天性・その他~
  NAKAMURA KENSUKE
  獣医麻酔外科学雑誌, Dec. 2012, Japanese
  [Invited]
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ病変における炎症性サイトカインの発現解析
  TAMURA YU; OTA HIROSHI; MURAKAMI MASAHIRO; BANDULA KUMARA WR; SUE YEE LIM; OSUGA TATSUYUKI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 31 Aug. 2012, Japanese
• 下垂体性副腎皮質機能亢進症と中枢性尿崩症を併発し,放射線治療を行った下垂体腫瘍の犬の1例
  TAMURA YU; YAMASAKI MASAHIRO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2012, Japanese
• 腱索断裂を伴う僧帽弁閉鎖不全症の小型犬に僧帽弁形成術を施行した1例
  SAWADA TAMOTSU; MAETANI SHIGEKI; TAMAI SATOSHI; KISHIHARA KEIICHIRO; ITAMI TAKAHARU; KONDO SHOICHI; NAKAMURA KENSUKE; MIZUNO MASASHI; HARADA KAYOKO; UCHIDA SHUHEI; UECHI MASAMI
  北海道獣医師会雑誌, 10 Aug. 2012, Japanese
• 両側性子宮分節性無形成から片側性子宮角捻転に至った犬の1例
  YOKOYAMA NOZOMI; NAKAMURA KENSUKE; KIMURA TAKASHI; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2012, Japanese
• 肝静脈血流波形解析による右心不全の重症度評価
  NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2012, Japanese
• 麻酔後の全脳虚血が疑われた2症例のMRI所見
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2012, Japanese
• 下垂体性副腎皮質機能亢進症とクロム親和性細胞腫を併発した犬の1例
  KANAZAWA TOMONORI; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2012, Japanese
• 好酸球増多症候群を伴った消化器型リンパ腫の猫の1例
  TOOYAMA NOBUO; HOSOYA KENJI; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2012, Japanese
• 房室ブロックの犬2例に認められた低速僧帽弁逆流と高速左室駆出血流
  NAKAMURA KENSUKE; TOOYAMA NOBUO; UENO TAKASHI; MORISHITA KEITARO; OSUGA TATSUYUKI; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 01 Jun. 2012, Japanese
• CT検査を実施した肝葉捻転の犬の2例
  MORISHITA KEITARO; HOSOYA KENJI; TAKAGI SATOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 01 Jun. 2012, Japanese
• 犬の頭蓋内疾患診断における経頭蓋超音波ドプラ法の有用性の検討
  SASAOKA KAZUYOSHI; NAKAMURA KENSUKE; MORISHITA KEITARO; OSUGA TATSUYUKI; LIM SUE YEE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 01 Jun. 2012, Japanese
• 診断装置によるソノポレーションの特徴と条件設定
  SASAKI NOBORU; NAKAMURA KENSUKE; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI
  超音波医学, 15 Mar. 2012, Japanese
• 日本小動物獣医学会教育講演「犬の肝腫瘤性病変の診断と治療指針」造影超音波検査による肝腫瘤性病変診断の最前線
  中村健介
  平成23年度日本獣医師会獣医学術学会年次大会, 05 Feb. 2012, Invited oral presentation
  [Invited], [Domestic Conference]
• 心エコー図検査にて心内膜心筋線維症と診断した猫4例
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; MURAKAMI MASAHIRO; MATSUYAMA ARATA; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 01 Dec. 2011, Japanese
• 犬の肝臓腫瘤診断におけるソナゾイド造影超音波検査―4年間の検討―
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; HOSOYA KENJI; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 18 Nov. 2011, Japanese
• 小型犬における下垂体性副腎皮質機能亢進症の超音波診断基準
  MATSUYAMA ARATA; TOOYAMA NOBUO; MURAKAMI MASAHIRO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 18 Nov. 2011, Japanese
• 活性型ビタミンD₃外用薬(マキサカルシトール軟膏)常用患者の飼育犬に認められた高カルシウム血症
  TOOYAMA NOBUO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 18 Nov. 2011, Japanese
• 超音波診断装置および超音波造影剤による抗がん剤の抗腫瘍効果の増強
  SASAKI NOBORU; NAKAMURA KENSUKE; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI
  超音波医学, 15 Nov. 2011, Japanese
• 犬の慢性腸炎症例の結腸粘膜における細胞接着分子クローディンの発現変動
  OTA HIROSHI; TAMURA YU; LIM SUE YEE; KUMARA WR BANDULA; MURAKAMI MASAHIRO; SASAKI NOBORU; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 31 Aug. 2011, Japanese
• プロテオーム解析を用いた犬リポ蛋白質測定法の評価
  SUDO ASUKA; YAMASAKI MASAHIRO; NAKAMURA KENSUKE; SASAKI NOBORU; MURAKAMI MASAHIRO; KUMARA W.R. BANDULA; TAMURA YU; LIM SUE YEE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 31 Aug. 2011, Japanese
• 犬の慢性腸炎症例の腸粘膜におけるIL‐17A遺伝子の発現解析
  TAKADA KANAE; OTA HIROSHI; NAKAMURA KENSUKE; SASAKI NOBORU; MURAKAMI MASAHIRO; KUMARA W.R. BANDULA; TAMURA YU; LIM SUE YEE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 31 Aug. 2011, Japanese
• 超音波診断装置および超音波造影剤による抗がん剤のソノポレーションの抗腫瘍効果
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; BANDULA KUMARA WR; LIM SUE YEE; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 31 Aug. 2011, Japanese
• 心エコー図検査とD‐dimer測定により診断し得た肺血栓塞栓症の犬の1例
  OGI NAOKO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2011, Japanese
• 気腫性胆嚢炎の犬の1例
  TAMURA YU; YAMASAKI MASAHIRO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2011, Japanese
• 犬の僧房弁閉鎖不全症におけるLeft atrial tracking法を用いた左心房機能評価の有用性
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2011, Japanese
• 犬の心疾患におけるLeft atrial tracking法を用いた左心房機能評価の有用性
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 01 Jun. 2011, Japanese
• 仮想気管支鏡検査が診断に有用であった肺葉捻転の犬の2例
  MURAKAMI MASAHIRO; NAKAMURA KENSUKE; SASAKI NOBORU; MATSUYAMA SHIN; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 01 Jun. 2011, Japanese
• 犬の肝カルチノイド4症例におけるソナゾイド造影超音波検査所見
  NAKAMURA KENSUKE; HOSOYA KENJI; YAMASAKI MASAHIRO; OTA HIROSHI; YEE LIM SUE; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 01 Jun. 2011, Japanese
• パルス超音波と微小気泡によるシスプラチンの抗腫瘍効果増強の基礎的知見
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI
  超音波医学, 15 May 2011, Japanese
• 診断用超音波とマイクロバブルによるドラッグデリバリーのin vitroでの最適化
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BADULA; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI
  超音波医学, 15 Apr. 2011, Japanese
• 二次性赤血球増加症を伴ったEisenmenger症候群の犬に対するシルデナフィルの有効性の検討
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  日本獣医皮膚科学会学術大会・総会, 11 Mar. 2011, Japanese
• 超音波とマイクロバブルによるドラッグデリバリーに薬剤濃度とバブル濃度が及ぼす影響
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BANDULA; TAMURA YU; YEE LIM SUE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医皮膚科学会学術大会・総会, 11 Mar. 2011, Japanese
• シトシンアラビノシドにより良好に維持されている特発性脳炎の犬の2例
  OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医皮膚科学会学術大会・総会, 11 Mar. 2011, Japanese
• 第二世代超音波造影剤ソナゾイドを用いた造影超音波検査による犬の肝臓および脾臓腫瘤の診断に関する研究
  NAKAMURA KENSUKE; TAKAGI SATOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; TAMURA YU; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 01 Mar. 2011, Japanese
• ガストリン産生性カルチノイドに対し徐放性オクトレオチド製剤を使用した犬の1例
  HOSOYA KENJI; KANEKO KAZUNARI; TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE; TAKAGI SATOSHI; OKUMURA MASAHIRO
  動物臨床医学会年次大会プロシーディング, 19 Nov. 2010, Japanese
• 微小気泡存在下での診断用超音波照射によるシスプラチンの抗腫瘍効果増強の検討
  SASAKI AZUMA; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; BANDULA KUMARA; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 01 Sep. 2010, Japanese
• 犬のガストリノーマに対してサンドスタチンLARを投与した1例
  KANEKO KAZUNARI; HOSOYA KENJI; TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE; KITAMURA JIRO; TAKAGI SATOSHI; OKUMURA MASAHIRO
  北海道獣医師会雑誌, 10 Aug. 2010, Japanese
• 心房中隔欠損症と僧帽弁閉鎖不全症を併発した犬の1例
  OGI NAOKO; NAKAMURA KENSUKE; MATSUYAMA SHIN; HASHIBA MINORU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2010, Japanese
• 造影3DCTにより確定診断し得た犬の重複大動脈弓の1例
  MATSUYAMA SHIN; KANEKO KAZUNARI; YAMANE YOSHIHISA; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2010, Japanese
• リンパ管拡張症を疑う胸水貯留に続発して肺葉捻転を起こした犬の1例
  MURAKAMI MASAHIRO; NAKAMURA KENSUKE; SASAKI AZUMA; OTA HIROSHI; YAMASAKI MASAHIRO; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2010, Japanese
• 胆嚢粘液嚢腫の犬19症例の回顧的解析
  MURATA NAOMITSU; TAKAGI SATOSHI; HOSOYA KENJI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI; SATO KOTA; OKUMURA MASAHIRO
  北海道獣医師会雑誌, 10 Aug. 2010, Japanese
• 造影超音波検査により病変の特定が可能であったインスリノーマの犬2例
  NAKAMURA KENSUKE; TAKAGI SATOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 10 Aug. 2010, Japanese
• 二次性赤血球増加症を伴ったEisenmenger症候群の犬に対するシルデナフィルの有効性の検討
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 20 Jul. 2010, Japanese
• 超音波診断装置と微小気泡によるシスプラチンの抗腫瘍効果の増強
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BANDULA; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI
  超音波医学, 15 Apr. 2010, Japanese
• 外科手術を実施した犬の肝細胞癌17症例の検討
  TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; NAKAMURA KENSUKE; OSAKI TOMOHIRO; HOSOYA YOSHITSUGU; OKUMURA MASAHIRO
  獣医麻酔外科学雑誌, 10 Dec. 2009, Japanese
• ボストンテリアの脾臓捻転の1症例
  OTA HIROSHI; TAKAGI SATOSHI; NAKAZAWA SHIGEKI; MURAKAMI MASAHIRO; SASAKI AKIRA; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2009, Japanese
• 気管支内視鏡検査で気管支狭窄を認めた慢性発咳の犬の1症例
  SASAKI AKIRA; OTA HIROSHI; ANDO REMI; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2009, Japanese
• 本態性高Na血症が疑われた神経症状を示した犬と猫の2例
  MURAKAMI MASAHIRO; OTA HIROSHI; NAKAMURA KENSUKE; SASAKI AKIRA; YAMASAKI MASAHIRO; ETO MASAYUKI; KOSEKI YUMI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2009, Japanese
• 筋炎の併発が疑われた劇症型重症筋無力症の犬の1例
  UKAI AYAKO; YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; NAKAO KIICHI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2009, Japanese
• 犬の肝臓腫瘤28結節に対するソナゾイド造影超音波検査
  NAKAMURA KENSUKE; TAKAGI SATOSHI; SASAKI AKIRA; MURAKAMI MASAHIRO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2009, Japanese
• 犬の脾臓腫瘤におけるソナゾイド造影超音波検査の有用性
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 2009, Japanese
• 犬のリンパ球プラズマ細胞性腸炎における細胞接着分子クローディンの発現変動
  OTA HIROSHI; MURAKAMI MASAHIRO; KUMARA BANDURA; SASAKI NOBORU; NAKAMURA KENSUKE; KO KOJAKU; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 2009, Japanese
• SLEが疑われた血球貪食症候群の猫の1例
  YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; SASAKI AZUMA; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医師会三学会年次大会講演要旨集, 25 Dec. 2008, Japanese
• 腹膜播種を疑う卵巣腫瘍のイヌの3例
  TAMURA JUN; TOMINAGA MAKIKO; NAKAMURA KENSUKE; MAETANI SHIGEKI; HIRAYAMA KAZUKO; TANIYAMA HIROYUKI; KADOSAWA TSUYOSHI
  獣医麻酔外科学雑誌, 20 Dec. 2008, Japanese
• 超音波検査により求めた正常なビーグル犬の甲状腺体積と体重の相関
  SASAKI NOBORU; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  動物臨床医学会年次大会プロシーディング, 14 Nov. 2008, Japanese
• 犬の消化菅粘膜におけるクローディン分子群の発現:十二指腸を中心として
  YAMAGUCHI TOMOO; OTA HIROSHI; NAKAMURA KENSUKE; O KOJAKU; SASAKI HIGASHI; YOSHIKAWA MUNEYOSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 05 Sep. 2008, Japanese
• 犬の肝臓における造影超音波検査の有用性の検討
  NAKAMURA KENSUKE; SASAKI HIGASHI; YOSHIKAWA MUNEYOSHI; O KOJAKU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  日本獣医学会学術集会講演要旨集, 05 Sep. 2008, Japanese
• SLEが疑われた血球貪食症候群の猫の1例
  YOSHIKAWA MUNEYOSHI; SHODA MASATO; NAKAMURA KENSUKE; SASAKI NOBORU; OTA HIROSHI; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2008, Japanese
• エリスロポエチン依存性の二次性多血症が疑われた腎リンパ腫の犬の1例
  SASAKI AZUMA; YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMAZAKI MASAHIRO; SATO KOTA; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2008, Japanese
• 抗リン脂質抗体症候群が疑われた不明熱のミニチュア・ダックスフンドの1例
  NAKAMURA KENSUKE; SASAKI NOBORU; YOSHIKAWA MUNEYOSHI; OTA HIROSHI; YAMAZAKI MASAHIRO; OBA MASAYO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2008, Japanese
• 第二世代超音波造影剤ソナゾイドによる肝臓腫瘤の診断
  NAKAMURA KENSUKE; OTA HIROSHI; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 20 Jun. 2008, Japanese
• クエン酸シルデナフィルが奏功した逆行性動脈管開存症のイヌの2例
  NAKAMURA KENSUKE; MORISHITA KEITARO; NAKADE TETSUYA; TAKIGUCHI MITSUYOSHI
  日本獣医師会三学会年次大会講演要旨集, 2008, Japanese
• 第二世代超音波造影剤ソナゾイドの小動物臨床への応用に関する基礎的検討
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE
  獣医麻酔外科学雑誌, 20 Nov. 2007, Japanese
• 犬の膵疾患における超音波ヒストグラム解析の有用性に関する基礎的研究
  NAKAMURA KENSUKE; MORISHITA KEITARO; TAKIGUCHI MAKI
  日本獣医学会学術集会講演要旨集, 27 Aug. 2007, Japanese
• メソトレキセートが奏功したステロイド抵抗性炎症性腸疾患の犬の1例
  MORISHITA KEITARO; NAKAMURA KENSUKE; HIRAYAMA KAZUKO; TANIYAMA HIROYUKI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2007, Japanese
• クエン酸シルデナフィルが奏功した逆行性動脈管開存症の犬の2例
  NAKAMURA KENSUKE; MORISHITA KEITARO; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2007, Japanese
• デジタルX線(DR)撮影下における非選択的血管造影法により逆行性動脈管開存症と診断された犬の2例
  MORISHITA KEITARO; NAKAMURA KENSUKE; NAKAIDE TETSUYA; TAKIGUCHI MAKI
  日本獣医循環器学会抄録, 2007, Japanese
• 十二指腸に閉塞性病変を伴った炎症性腸疾患の犬の1例
  NAKAMURA KENSUKE; MORISHITA KEITARO; RENZAWA TSUYOSHI; HIRAYAMA KAZUKO; TANIYAMA HIROYUKI; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2006, Japanese
• デジタルX線(DR)撮影下における非選択的血管造影法により逆行性動脈管開存症と診断された犬の1例
  TOOYAMA NOBUO; NAKAMURA KENSUKE; MORISHITA KEITARO; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2006, Japanese
• 犬の慢性リンパ性白血病:メルファラン継続投与による良好な長期コントロールの1例
  HIROSE TAKESHI; NAKAMURA KENSUKE; MORISHITA KEITARO; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2006, Japanese
• 犬の肺動脈狭窄におけるバルーン弁口拡大術
  TATEMATSU AYUMI; IKEZONO YUKIKO; NAKAMURA KENSUKE; INOUE AIKA; MAETANI SHIGEKI; NAKAIDE TETSUYA
  北海道獣医師会雑誌, 01 Aug. 2006, Japanese
• I型偽性低アルドステロン症が疑われた犬の1例
  MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI
  北海道獣医師会雑誌, 01 Aug. 2006, Japanese
• 肉芽腫性胃炎により幽門狭窄症を呈したイヌの一例
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI
  獣医麻酔外科学雑誌, 20 Jun. 2006, Japanese
• 超音波検査により粥状動脈硬化症と診断された犬の1例
  NAKAMURA KENSUKE; KITAMURA JIRO; TAKIGUCHI MAKI
  北海道獣医師会雑誌, 01 Aug. 2005, Japanese
• 頻回の内視鏡下バルーン拡張術が功を奏した食道狭窄の猫の1例
  MIWA ERIKO; MIYATA KAYOKO; MACHIDA TATSUHIKO; NAKAMURA KENSUKE; NAKAIDE TETSUYA; TAKIGUCHI MAKI
  北海道獣医師会雑誌, 01 Aug. 2005, Japanese
• 血管超音波検査によりアテローム性動脈硬化症が疑われた犬の一例
  NAKAMURA KENSUKE; KITAMURA JIRO; TAKIGUCHI MAKI
  獣医麻酔外科学雑誌, 20 Jun. 2005, Japanese
• イオノフォア抗生物質サリノマイシンのBabesia gibsoniに対する効果
  YAMASAKI MASAHIRO; NAKAMURA KENSUKE; YAMATO OSAMU; MAEDE YOSHIMITSU
  日本寄生虫学会大会プログラム・抄録集, Feb. 2005, Japanese

• 伴侶動物獣医療実習, 2024年, 学士課程, 獣医学部
• 臨床疾病学特論, 2024年, 博士後期課程, 獣医学院
• 伴侶動物夜間・救急獣医療実習, 2024年, 学士課程, 獣医学部
• 獣医内科学特論, 2024年, 博士後期課程, 獣医学院
• プレクリニカル実習, 2024年, 学士課程, 獣医学部
• 総合専門臨床特論, 2024年, 博士後期課程, 獣医学院
• 伴侶動物獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 伴侶動物獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 伴侶動物獣医療実習Ⅱ, 2024年, 学士課程, 獣医学部
• 伴侶動物獣医療実習Ⅱ, 2024年, 学士課程, 獣医学部
• 夜間・救急獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 夜間・救急獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 総合獣医療実習, 2024年, 学士課程, 獣医学部
• 総合獣医療実習, 2024年, 学士課程, 獣医学部
• アドバンスト演習, 2024年, 学士課程, 獣医学部
• アドバンスト演習, 2024年, 学士課程, 獣医学部
• アドバンスト演習, 2024年, 学士課程, 獣医学部
• プレクリニカル実習, 2024年, 学士課程, 獣医学部
• 放射線獣医療・画像診断学, 2024年, 学士課程, 獣医学部

• JAPANESE SOCIETY OF ECHOCARDIOGRAPHY
• THE JAPANESE CIRCULATION SOCIETY
• JAPANESE SOCIETY OF CLINICAL VETERINARY MEDICINE
• THE JAPANESE SOCIETY OF VETERINARY IMAGINGS
• JAPANESE SOCIETY OF VETERINARY CARDIOLOGY
• JAPAN VETERINARY MEDICAL ASSOCIATION
• JAPANESE SOCIETY OF VETERINARY SCIENCE

• 僧帽弁形成術の予後を左右する残存肺高血圧症の発生を肺動脈内血流から予測する
  科学研究費助成事業
  01 Apr. 2023 - 31 Mar. 2026
  中村 健介
  本研究の初年度である2023年度は、Blood Speckle Imagingがそもそも犬において利用可能であるのかを正常な犬を用いて検証した。その結果、人で用いられている条件からの変更が必要なものの、概ね問題なく肺動脈内の血流を評価することが可能であることが明らかとなった。
  続いて臨床研究について、研究期間中に10例の僧帽弁逆流症例に対して僧帽弁形成術を実施した。そのうち１例では術後早期に発症した悪性腫瘍のため充分なフォローアップができず、９例を本研究の対象とした。９例中、術後肺高血圧症を発症したのは１例のみであり、人の患者において報告されている術後肺高血圧症発生率約50%を大きく下回るものであった。そのため、現状では術後肺高血圧症の発症リスク因子を評価することは不可能であるが、本研究で最も着目しているBlood Speckle Imagingを用いた肺動脈内渦流の評価については、当該症例において術前後ともに異常が認められており、本手法の有用性が伺える結果が得られたと考えている。また、術前から肺高血圧症を有していたのは約半数の４例であったが、いずれの症例においても同様にBlood Speckle Imagingによって異常な肺動脈内渦流が確認され、本手法が肺高血圧症診断の簡便で有用な診断方法となりうる可能性が考えられている。
  なお、肺高血圧症モデル動物を用いた実験的な検討については、他研究との兼ね合いから実施できておらず、次年度以降に実施する予定である。
  日本学術振興会, 基盤研究(C), 北海道大学, 23K05548
• 肺内短絡血管は肺塞栓症の重症化に関与しているか
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  01 Apr. 2019 - 31 Mar. 2022
  中村 健介
  本研究計画では犬を用いて人工的に肺塞栓を起こし、それに伴う血行動態の変化を心エコー図検査による右心機能指標の評価、さらには振盪生理食塩液を用いた contrast echoによる肺内短絡血管の有無を評価する予定であったが、所属機関における実験動物施設の制限等に伴い、使用動物をウサギに変更せざるを得なかった。犬についてはこれまで多くの経験を有するが、ウサギに関しては正常値の確立からはじめる必要があり、本年度はまず正常なウサギを用いて肺塞栓症に関連する心エコー図検査による右心機能指標の基準値計測を行うとともに、bubble contrast echoを行い正常なウサギにおける肺内短絡血管の有無を評価した。その結果、ウサギにおいても右心機能指標が再現性高く評価可能であること、正常なウサギでは肺内短絡血管が存在しないことが明らかとなった。
  続いて、ウサギの頸静脈に血栓を形成し、これにより心エコー図検査による右心機能指標が変動、さらには肺内短絡血管の疎通の有無を評価した。その結果、頸静脈での血栓形成に伴い右心機能指標がわずかに変動することが明らかとなった。一方、bubble contrast echoの結果、肺内短絡血管の疎通は認められなかった。肺塞栓を誘発していないのにも関わらず右心機能指標に変動が生じた理由については今後病理学的検査により原因を追及する予定であるが、微細な血栓の塞栓、あるいは血栓から何らかの血管作動性物質が産生されている可能性が考えられた。
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), University of Miyazaki, 19K06422
• 肺内短絡血管は肺塞栓症の重症化に関与しているか
  科学研究費補助金（基板研究（Ｃ））
  Apr. 2019 - Mar. 2022
  中村健介
  文部科学省, Principal investigator, Competitive research funding
• How close can an echocardiogram get to right heart function? -A study using a pulmonary hypertension model
  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
  01 Apr. 2016 - 31 Mar. 2020
  Nakamura Kensuke
  During the period of the study, animal models of pulmonary hypertension were created and the relationship between pulmonary artery pressure (PAP) and echocardiographic right heart function indices was determined. The results showed that some of the right heart function indices reflect well the pulmonary artery pressure. In particular, it was demonstrated that the newly developed index RV-SD, which assesses the synchronization of right ventricular contraction, may be the most useful indicator of PAP. Furthermore, the RV-SD was similarly useful in dogs with naturally occurring pulmonary hypertension.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), 16K18800
• 右心機能に心エコーがどこまで迫れるのか？－肺高血圧症モデルを用いた検討－
  科学研究費補助金(若手研究(B))
  Apr. 2016 - Mar. 2019
  中村健介
  文部科学省, Principal investigator, Competitive research funding
• Novel cancer therapy for spontaneous animal tumor using ultrasound irradiation with microbubble
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
  01 Apr. 2014 - 31 Mar. 2017
  TAKIGUCHI MITSUYOSHI; KUDO Nobuki
  This study revealed that sonoporation, the combination of ultrasound and microbubbles, enhances the antitumor effect of a chemotherapeutic agent with minimum invasiveness in preclinical canine thyroid and bladder cancer models. Moreover, the results of this study clarified the mechanism of sonoporation. This preclinical study may contribute to establish sonoporation as a theranostic tool in cancer therapy.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 26292155
• Toward the development of prognostic indicator and treatment strategy of mitral regurgitation focusing on atrial function
  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
  01 Apr. 2013 - 31 Mar. 2017
  Nakamura Kensuke; OSUGA Tatsuyuki
  In this study, 1) normal reactivity of left atrium to the volume overload, 2) normal reactivity of left atrium to cardiovascular drugs, 3) changes of left atrial function in cardiac disease, were evaluated. The left atrium has the potential capacity to become double reacting to volume overload and drugs. In the earlier coarse of cardiac disease, the left atrial function increases to adapt the worse condition. However, it decreases gradually with the progression of disease severity, and patients finally die when the worsening of atrial function cross over the certain limit. Left atrium may act as the last defense in animals with cardiac disease.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 25850203
• 新たな心エコー図検査スペックルトラッキング法による心房機能解析 〜負荷に対して心房は如何に反応するのか〜
  研究助成（個人研究の部）
  Apr. 2012 - Mar. 2013
  中村健介
  栗林育英学術財団, Principal investigator, Competitive research funding
• 心房機能解析は心不全発症を予測する新規検査法となり得るか
  平成24年度総長室事業推進経費（公募型プロジェクト研究等支援経費）「若手研究者自立支援」
  Apr. 2012 - Mar. 2013
  中村健介
  北海道大学, Principal investigator, Competitive research funding
• 肝臓腫瘤診断における造影超音波検査の応用に関する研究ー診断から分化度の評価へー
  特別研究員奨励費
  Apr. 2010 - Mar. 2012
  中村健介
  日本学術振興会, Principal investigator, Competitive research funding
• 肝臓腫瘤診断における造影超音波検査の応用に関する研究―診断から分化度の評価へ―
  Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows
  2010 - 2011
  中村 健介
  本研究では、ソナゾイド造影超音波検査による犬の肝臓腫瘤診断法を確立するとともに、特に肝細胞癌の分化度を評価する方法を確立するべく研究を行った。研究期間中に、対象となった肝臓腫瘤症例は67症例であり、11例が良性の結節性過形成、56例が悪性腫瘍であった。悪性腫瘍のうち、肝細胞癌が33例と最も多く、次いで血管肉腫4例、肝カルチノイド4例、胆管癌3例、組織球肉腫3例、その他9例であった。動脈相においては、良性腫瘤の多くがIsoに分類された(9/11,81.8%)のに対して、悪性腫瘍の多くがHyper(25/56,44.6%)もしくはHypo(18/56,32.1%)に分類された。Hyperと分類された症例の多くは癌腫であった(22/25,88.0%)。門脈相においては良性腫瘤の全例がIsoに分類された(11/11,100%)のに対して、悪性腫瘍の多くがHypoに分類された(40/56,71.4%)。ただし、肝細胞癌に却いては、Hypoに分類される例は約半数にとどまり(18/33,54.5%)、造影増強される例も比較的多く認められた。実質相において、良性腫瘤の全例が周囲の正常実質と同程度に造影され、Isoに分類された(11/11,100%)のに対して、悪性腫瘍の多くが明瞭な造影欠損を示し、Hypoと分類された(51/56,91%)。ただし、肝細胞癌においては、実質相で造影増強を示しIsoに分類される例も存在した(3/33,9.1%)。以上の様に、本研究により犬の肝臓腫瘤診断においてソナゾイド造影超音波検査が非常に優れた精度を持つことが明らかとなった。肝細胞癌の33例中、実質相においてIsoとなった症例は3例と非常に少ない割合であったがこれらの症例においてはHypoに分類された症例よりも、免疫染色によりKupffer細胞数が多い傾向が認められた。しかしながら一方で、今回の研究対象となった33症例の全てが病理組織学的検査により高分化型肝細胞癌と分類され、分化度に応じた比較は不可能であった。ただし、その中でも肝細胞癌を再発した症例が複数存在しており、病理検査の限界も見て取れた。従って、今後これらの症例に対して追跡調査を行い、肝細胞癌の再発率や予後とKupffer細胞数ならびに造影超音波検査所見との間に存在する因果関係を明らかとしたい。
  Japan Society for the Promotion of Science, Grant-in-Aid for JSPS Fellows, Hokkaido University, 10J05227

• 医用データ処理装置、医用データ処理方法、及び超音波診断装置
  Patent right, 高木一也; 滝口満喜; 中村健介
  特願PCT/JP2013/004697