Researcher Database

Toshinori Endo
Faculty of Information Science and Technology Bioengineering and Bioinformatics Bioinformatics
Professor

Researcher Profile and Settings

Affiliation

  • Faculty of Information Science and Technology Bioengineering and Bioinformatics Bioinformatics

Job Title

  • Professor

Degree

  • Ph.D. in Genetics(The Graduate University for Advanced Studies)
  • Master.of Science(The University of Tokyo)

URL

Research funding number

  • 00323692

J-Global ID

Research Interests

  • prediction of function by machine learning   gene structure and function   Information Biology   

Research Areas

  • Life sciences / Evolutionary biology
  • Life sciences / Biodiversity and systematics
  • Life sciences / Functional biochemistry
  • Life sciences / Genomics
  • Nanotechnology/Materials / Molecular biochemistry
  • Informatics / Biological, health, and medical informatics

Academic & Professional Experience

  • 2004/04 - Today Hokkaido University Graduate School of Information Science and Technology Professor
  • 2017/04 - 2018/03 Hokkaido University
  • 2014/04 - 2015/03 Hokkaido University Division of Bioengineering and Bioinformatics Chair
  • 2014/04 - 2015/03 Hokkaido University Department of Informatics and Electronics, Faculty of Engineering Department Chair
  • 2008/04 - 2009/03 Hokkaido University Graduate School of Information Science and Technology
  • 2000/04 - 2004/03 Tokyo Medical and Dental University Lecturer
  • 2000 - 2004 Lecturer,Tokyo Medical and Dental University
  • 1999/05 - 2000/03 RIKEN Genome Sciences Center Researcher
  • 1999 - 2000 Researcher,RIKEN GSC
  • 1997/04 - 1999/04 日本学術振興会 特別研究員
  • 1997 - 1999 Researcher
  • 1996/04 - 1997/03 新エネルギー・産業技術開発機構 研究員
  • 1996 - 1997 Researcher,NEDO, Researcher

Education

  • 1993/04 - 1996/03  The Graduate University for Advanced Studies  School of Life Science  Department of Genetics
  •        - 1996  The Graduate University for Advanced Studies  Graduate School, Division of Life Science  Department of Genetics
  • 1990/04 - 1993/03  University of Tokyo  School of Science  Department of Biophysics and Biochemistry
  •        - 1993  The University of Tokyo  Graduate School, Division of Science  Department of Biophysics and Biochemistry
  •        - 1992  The University of Tokyo  Graduate School, Division of Science  Department of Biophysics and Biochemistry
  • 1986/04 - 1990/03  Tokyo University  Faculty of Science  Department of Biophysics and Biochemistry
  •        - 1990  The University of Tokyo  Faculty of Science  Department of Biophysics and Biochemistry

Association Memberships

  • 日本進化学会   日本情報処理学会   日本バイオインフォマティクス学会   生き物文化誌学会   Society for Molecular Biology and Evolution   日本遺伝学会   Japan Society of Bioinformatics   Society of Biosophia Studies   Society for Molecular Biology and Evolution   Japanese Society for Genetics   

Research Activities

Published Papers

  • Keisuke Ueno, Katsuhiko Mineta, Kimihito Ito, Toshinori Endo
    BMC STRUCTURAL BIOLOGY 12 5  1472-6807 2012/04 [Refereed][Not invited]
     
    Background: Structural genomics approaches, particularly those solving the 3D structures of many proteins with unknown functions, have increased the desire for structure-based function predictions. However, prediction of enzyme function is difficult because one member of a superfamily may catalyze a different reaction than other members, whereas members of different superfamilies can catalyze the same reaction. In addition, conformational changes, mutations or the absence of a particular catalytic residue can prevent inference of the mechanism by which catalytic residues stabilize and promote the elementary reaction. A major hurdle for alignment-based methods for prediction of function is the absence (despite its importance) of a measure of similarity of the physicochemical properties of catalytic sites. To solve this problem, the physicochemical features radially distributed around catalytic sites should be considered in addition to structural and sequence similarities. Results: We showed that radial distribution functions (RDFs), which are associated with the local structural and physicochemical properties of catalytic active sites, are capable of clustering oxidoreductases and transferases by function. The catalytic sites of these enzymes were also characterized using the RDFs. The RDFs provided a measure of the similarity among the catalytic sites, detecting conformational changes caused by mutation of catalytic residues. Furthermore, the RDFs reinforced the classification of enzyme functions based on conventional sequence and structural alignments. Conclusions: Our results demonstrate that the application of RDFs provides advantages in the functional classification of enzymes by providing information about catalytic sites.
  • Mia Nakachi, Ayako Nakajima, Mamoru Nomura, Kouki Yonezawa, Keisuke Ueno, Toshinori Endo, Kazuo Inaba
    MOLECULAR REPRODUCTION AND DEVELOPMENT 78 (7) 529 - 549 1040-452X 2011/07 [Not refereed][Not invited]
     
    In this study, we performed extensive proteomic analysis of sperm from the ascidian Ciona intestinalis. Sperm were fractionated into heads and flagella, followed by further separation into Triton X-100-soluble and -insoluble fractions. Proteins from each fraction and whole sperm were separated by isoelectric focusing using two different pH ranges, followed by SDS-PAGE at two different polyacrylamide concentrations. In total, 1,294 protein spots representing 304 non-redundant proteins were identified by mass spectrometry (MALDI-TOF). On comparison of the proteins in each fraction, we were able to identify the proteins specific to different sperm compartments. Further comparison with the testis proteome allowed the pairing of proteins with sperm-specific functions. Together with information on gene expression in developing embryos and adult tissues, these results provide insight into novel cellular and functional aspects of sperm proteins, such as distinct localization of actin isoforms, novel Ca-2 vertical bar-binding proteins in axonemes, localization of testis-specific serine/threonine kinase, and the presence of G-protein coupled signaling and ubiquitin pathway in sperm flagella. Mol. Reprod. Dev. 78: 529-549, 2011. (C) 2011 Wiley-Liss, Inc.
  • Mia Nakachi, Ayako Nakajima, Mamoru Nomura, Kouki Yonezawa, Keisuke Ueno, Toshinori Endo, Kazuo Inaba
    MOLECULAR REPRODUCTION AND DEVELOPMENT 78 (7) 529 - 549 1040-452X 2011/07 [Refereed][Not invited]
     
    In this study, we performed extensive proteomic analysis of sperm from the ascidian Ciona intestinalis. Sperm were fractionated into heads and flagella, followed by further separation into Triton X-100-soluble and -insoluble fractions. Proteins from each fraction and whole sperm were separated by isoelectric focusing using two different pH ranges, followed by SDS-PAGE at two different polyacrylamide concentrations. In total, 1,294 protein spots representing 304 non-redundant proteins were identified by mass spectrometry (MALDI-TOF). On comparison of the proteins in each fraction, we were able to identify the proteins specific to different sperm compartments. Further comparison with the testis proteome allowed the pairing of proteins with sperm-specific functions. Together with information on gene expression in developing embryos and adult tissues, these results provide insight into novel cellular and functional aspects of sperm proteins, such as distinct localization of actin isoforms, novel Ca-2 vertical bar-binding proteins in axonemes, localization of testis-specific serine/threonine kinase, and the presence of G-protein coupled signaling and ubiquitin pathway in sperm flagella. Mol. Reprod. Dev. 78: 529-549, 2011. (C) 2011 Wiley-Liss, Inc.
  • Katsuhiko Mineta, Yasuko Yamamoto, Yuji Yamazaki, Hiroo Tanaka, Yukiyo Tada, Kuniaki Saito, Atsushi Tamura, Michihiro Igarashi, Toshinori Endo, Kosei Takeuchi, Sachiko Tsukita
    FEBS LETTERS 585 (4) 606 - 612 0014-5793 2011/02 [Refereed][Not invited]
     
    Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Structured summary: Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction) ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction) (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
  • Toshinori Endo, Keisuke Ueno, Kouki Yonezawa, Katsuhiko Mineta, Kohji Hotta, Yutaka Satou, Lixy Yamada, Michio Ogasawara, Hiroki Takahashi, Ayako Nakajima, Mia Nakachi, Mamoru Nomura, Junko Yaguchi, Yasunori Sasakura, Chisato Yamasaki, Miho Sera, Akiyasu C. Yoshizawa, Tadashi Imanishi, Hisaaki Taniguchi, Kazuo Inaba
    NUCLEIC ACIDS RESEARCH 39 (suppl) D807 - D814 0305-1048 2011/01 [Not refereed][Not invited]
     
    The Ciona intestinalis protein database (CIPRO) is an integrated protein database for the tunicate species C. intestinalis. The database is unique in two respects: first, because of its phylogenetic position, Ciona is suitable model for understanding vertebrate evolution; and second, the database includes original large-scale transcriptomic and proteomic data. Ciona intestinalis has also been a favorite of developmental biologists. Therefore, large amounts of data exist on its development and morphology, along with a recent genome sequence and gene expression data. The CIPRO database is aimed at collecting those published data as well as providing unique information from unpublished experimental data, such as 3D expression profiling, 2D-PAGE and mass spectrometry-based large-scale analyses at various developmental stages, curated annotation data and various bioinformatic data, to facilitate research in diverse areas, including developmental, comparative and evolutionary biology. For medical and evolutionary research, homologs in humans and major model organisms are intentionally included. The current database is based on a recently developed KH model containing 36 034 unique sequences, but for higher usability it covers 89 683 all known and predicted proteins from all gene models for this species. Of these sequences, more than 10 000 proteins have been manually annotated. Furthermore, to establish a community-supported protein database, these annotations are open to evaluation by users through the CIPRO website. CIPRO 2.5 is freely accessible at http://cipro.ibio.jp/2.5.
  • Predicted expansion of the claudin multigene family
    FEBS Letter doi:10.1016/j.febslet.2011.01.028  2011 [Not refereed][Not invited]
  • Toshinori Endo, Keisuke Ueno, Kouki Yonezawa, Katsuhiko Mineta, Kohji Hotta, Yutaka Satou, Lixy Yamada, Michio Ogasawara, Hiroki Takahashi, Ayako Nakajima, Mia Nakachi, Mamoru Nomura, Junko Yaguchi, Yasunori Sasakura, Chisato Yamasaki, Miho Sera, Akiyasu C. Yoshizawa, Tadashi Imanishi, Hisaaki Taniguchi, Kazuo Inaba
    NUCLEIC ACIDS RESEARCH 39 D807 - D814 0305-1048 2011/01 [Refereed][Not invited]
     
    The Ciona intestinalis protein database (CIPRO) is an integrated protein database for the tunicate species C. intestinalis. The database is unique in two respects: first, because of its phylogenetic position, Ciona is suitable model for understanding vertebrate evolution; and second, the database includes original large-scale transcriptomic and proteomic data. Ciona intestinalis has also been a favorite of developmental biologists. Therefore, large amounts of data exist on its development and morphology, along with a recent genome sequence and gene expression data. The CIPRO database is aimed at collecting those published data as well as providing unique information from unpublished experimental data, such as 3D expression profiling, 2D-PAGE and mass spectrometry-based large-scale analyses at various developmental stages, curated annotation data and various bioinformatic data, to facilitate research in diverse areas, including developmental, comparative and evolutionary biology. For medical and evolutionary research, homologs in humans and major model organisms are intentionally included. The current database is based on a recently developed KH model containing 36 034 unique sequences, but for higher usability it covers 89 683 all known and predicted proteins from all gene models for this species. Of these sequences, more than 10 000 proteins have been manually annotated. Furthermore, to establish a community-supported protein database, these annotations are open to evaluation by users through the CIPRO website. CIPRO 2.5 is freely accessible at http://cipro.ibio.jp/2.5.
  • Yutaka Satou, Katsuhiko Mineta, Michio Ogasawara, Yasunori Sasakura, Eiichi Shoguchi, Keisuke Ueno, Lixy Yamada, Jun Matsumoto, Jessica Wasserscheid, Ken Dewar, Graham B. Wiley, Simone L. Macmil, Bruce A. Roe, Robert W. Zeller, Kenneth E. M. Hastings, Patrick Lemaire, Erika Lindquist, Toshinori Endo, Kohji Hotta, Kazuo Inaba
    GENOME BIOLOGY 9 (10) R152  1474-760X 2008 [Not refereed][Not invited]
     
    Background: The draft genome sequence of the ascidian Ciona intestinalis, along with associated gene models, has been a valuable research resource. However, recently accumulated expressed sequence tag (EST)/cDNA data have revealed numerous inconsistencies with the gene models due in part to intrinsic limitations in gene prediction programs and in part to the fragmented nature of the assembly. Results: We have prepared a less-fragmented assembly on the basis of scaffold-joining guided by paired-end EST and bacterial artificial chromosome (BAC) sequences, and BAC chromosomal in situ hybridization data. The new assembly (115.2 Mb) is similar in length to the initial assembly (116.7 Mb) but contains 1,272 (approximately 50%) fewer scaffolds. The largest scaffold in the new assembly incorporates 95 initial-assembly scaffolds. In conjunction with the new assembly, we have prepared a greatly improved global gene model set strictly correlated with the extensive currently available EST data. The total gene number (15,254) is similar to that of the initial set (15,582), but the new set includes 3,330 models at genomic sites where none were present in the initial set, and 1,779 models that represent fusions of multiple previously incomplete models. In approximately half, 5'-ends were precisely mapped using 5'-full-length ESTs, an important refinement even in otherwise unchanged models. Conclusion: Using these new resources, we identify a population of non-canonical (non-GT-AG) introns and also find that approximately 20% of Ciona genes reside in operons and that operons contain a high proportion of single-exon genes. Thus, the present dataset provides an opportunity to analyze the Ciona genome much more precisely than ever.
  • Chisato Yamasaki, Katsuhiko Murakami, Yasuyuki Fujii, Yoshiharu Sato, Erimi Harada, Jun-Ichi Takeda, Takayuki Taniya, Ryuichi Sakate, Shingo Kikugawa, Makoto Shimada, Motohiko Tanino, Kanako O. Koyanagi, Roberto A. Barrero, Craig Gough, Hong-Woo Chun, Takuya Habara, Hideki Hanaoka, Yosuke Hayakawa, Phillip B. Hilton, Yayoi Kaneko, Masako Kanno, Yoshihiro Kawahara, Toshiyuki Kawamura, Akihiro Matsuya, Naoki Nagata, Kensaku Nishikata, Akiko Ogura Noda, Shin Nurimoto, Naomi Saichi, Hiroaki Sakai, Ryoko Sanbonmatsu, Rie Shiba, Mami Suzuki, Kazuhiko Takabayashi, Aiko Takahashi, Takuro Tamura, Masayuki Tanaka, Susumu Tanaka, Fusano Todokoro, Kaori Yamaguchi, Naoyuki Yamamoto, Toshihisa Okido, Jun Mashima, Aki Hashizume, Lihua Jin, Kyung-Bum Lee, Yi-Chueh Lin, Asami Nozaki, Katsunaga Sakai, Masahito Tada, Satoru Miyazaki, Takashi Makino, Hajime Ohyanagi, Naoki Osato, Nobuhiko Tanaka, Yoshiyuki Suzuki, Kazuho Ikeo, Naruya Saitou, Hideaki Sugawara, Claire O'Donovan, Tamara Kulikova, Eleanor Whitfield, Brian Halligan, Mary Shimoyama, Simon Twigger, Kei Yura, Kouichi Kimura, Tomohiro Yasuda, Tetsuo Nishikawa, Yutaka Akiyama, Chie Motono, Yuri Mukai, Hideki Nagasaki, Makiko Suwa, Paul Horton, Reiko Kikuno, Osamu Ohara, Doron Lancet, Eric Eveno, Esther Graudens, Sandrine Imbeaud, Marie Anne Debily, Yoshihide Hayashizaki, Clara Amid, Michael Han, Andreas Osanger, Toshinori Endo, Michael A. Thomas, Mika Hirakawa, Wojciech Makalowski, Mitsuteru Nakao, Nam-Soon Kim, Hyang-Sook Yoo, Sandro J. De Souza, Maria de Fatima Bonaldo, Yoshihito Niimura, Vladimir Kuryshev, Ingo Schupp, Stefan Wiemann, Matthew Bellgard, Masafumi Shionyu, Libin Jia, Danielle Thierry-Mieg, Jean Thierry-Mieg, Lukas Wagner, Qinghua Zhang, Mitiko Go, Shinsei Minoshima, Masafumi Ohtsubo, Kousuke Hanada, Peter Tonellato, Takao Isogai, Ji Zhang, Boris Lenhard, Sangsoo Kim, Zhu Chen, Ursula Hinz, Anne Estreicher, Kenta Nakai, Izabela Makalowska, Winston Hide, Nicola Tiffin, Laurens Wilming, Ranajit Chakraborty, Marcelo Bento Soares, Maria Luisa Chiusano, Yutaka Suzuki, Charles Auffray, Yumi Yamaguchi-Kabata, Takeshi Itoh, Teruyoshi Hishiki, Satoshi Fukuchi, Ken Nishikawa, Sumio Sugano, Nobuo Nomura, Yoshio Tateno, Tadashi Imanishi, Takashi Gojobori
    NUCLEIC ACIDS RESEARCH 36 (D) D793 - D799 0305-1048 2008/01 [Not refereed][Not invited]
     
    Here we report the new features and improvements in our latest release of the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/), a comprehensive annotation resource for human genes and transcripts. H-InvDB, originally developed as an integrated database of the human transcriptome based on extensive annotation of large sets of full-length cDNA (FLcDNA) clones, now provides annotation for 120 558 human mRNAs extracted from the International Nucleotide Sequence Databases (INSD), in addition to 54 978 human FLcDNAs, in the latest release H-InvDB_4.6. We mapped those human transcripts onto the human genome sequences (NCBI build 36.1) and determined 34 699 human gene clusters, which could define 34 057 (98.1%) protein-coding and 642 (1.9%) non-protein-coding loci; 858 (2.5%) transcribed loci overlapped with predicted pseudogenes. For all these transcripts and genes, we provide comprehensive annotation including gene structures, gene functions, alternative splicing variants, functional non-protein-coding RNAs, functional domains, predicted sub cellular localizations, metabolic pathways, predictions of protein 3D structure, mapping of SNPs and microsatellite repeat motifs, co-localization with orphan diseases, gene expression profiles, orthologous genes, proteinprotein interactions (PPI) and annotation for gene families. The current H-InvDB annotation resources consist of two main views: Transcript view and Locus view and eight sub-databases: the DiseaseInfo Viewer, H-ANGEL, the Clustering Viewer, G-integra, the TOPO Viewer, Evola, the PPI view and the Gene family/group.
  • Akihiro Matsuya, Ryuichi Sakate, Yoshihiro Kawahara, Kanako O. Koyanagi, Yoshiharu Sato, Yasuyuki Fujii, Chisato Yamasaki, Takuya Habara, Hajime Nakaoka, Fusano Todokoro, Kaori Yamaguchi, Toshinori Endo, Satoshi Oota, Wojciech Makalowski, Kazuho Ikeo, Yoshiyuki Suzuki, Kousuke Hanada, Katsuyuki Hashimoto, Momoki Hirai, Hisakazu Iwama, Naruya Saitou, Aiko T. Hiraki, Lihua Jin, Yayoi Kaneko, Masako Kanno, Katsuhiko Murakami, Akiko Ogura Noda, Naomi Saichi, Ryoko Sanbonmatsu, Mami Suzuki, Jun-Ichi Takeda, Masayuki Tanaka, Takashi Gojobori, Tadashi Imanishi, Takeshi Itoh
    NUCLEIC ACIDS RESEARCH 36 D787 - D792 0305-1048 2008/01 [Refereed][Not invited]
     
    Orthologs are genes in different species that evolved from a common ancestral gene by speciation. Currently, with the rapid growth of transcriptome data of various species, more reliable orthology information is prerequisite for further studies. However, detection of orthologs could be erroneous if pairwise distance-based methods, such as reciprocal BLAST searches, are utilized. Thus, as a sub-database of H-InvDB, an integrated database of annotated human genes (http://h-invitational.jp/), we constructed a fully curated database of evolutionary features of human genes, called Evola. In the process of the ortholog detection, computational analysis based on conserved genome synteny and transcript sequence similarity was followed by manual curation by researchers examining phylogenetic trees. In total, 18 968 human genes have orthologs among 11 vertebrates (chimpanzee, mouse, cow, chicken, zebrafish, etc.), either computationally detected or manually curated orthologs. Evola provides amino acid sequence alignments and phylogenetic trees of orthologs and homologs. In 'd(N)/d(S) view', natural selection on genes can be analyzed between human and other species. In 'Locus maps', all transcript variants and their exon/intron structures can be compared among orthologous gene loci. We expect the Evola to serve as a comprehensive and reliable database to be utilized in comparative analyses for obtaining new knowledge about human genes. Evola is available at http://www.h-invitational.jp/evola/.
  • Chisato Yamasaki, Katsuhiko Murakami, Yasuyuki Fujii, Yoshiharu Sato, Erimi Harada, Jun-Ichi Takeda, Takayuki Taniya, Ryuichi Sakate, Shingo Kikugawa, Makoto Shimada, Motohiko Tanino, Kanako O. Koyanagi, Roberto A. Barrero, Craig Gough, Hong-Woo Chun, Takuya Habara, Hideki Hanaoka, Yosuke Hayakawa, Phillip B. Hilton, Yayoi Kaneko, Masako Kanno, Yoshihiro Kawahara, Toshiyuki Kawamura, Akihiro Matsuya, Naoki Nagata, Kensaku Nishikata, Akiko Ogura Noda, Shin Nurimoto, Naomi Saichi, Hiroaki Sakai, Ryoko Sanbonmatsu, Rie Shiba, Mami Suzuki, Kazuhiko Takabayashi, Aiko Takahashi, Takuro Tamura, Masayuki Tanaka, Susumu Tanaka, Fusano Todokoro, Kaori Yamaguchi, Naoyuki Yamamoto, Toshihisa Okido, Jun Mashima, Aki Hashizume, Lihua Jin, Kyung-Bum Lee, Yi-Chueh Lin, Asami Nozaki, Katsunaga Sakai, Masahito Tada, Satoru Miyazaki, Takashi Makino, Hajime Ohyanagi, Naoki Osato, Nobuhiko Tanaka, Yoshiyuki Suzuki, Kazuho Ikeo, Naruya Saitou, Hideaki Sugawara, Claire O'Donovan, Tamara Kulikova, Eleanor Whitfield, Brian Halligan, Mary Shimoyama, Simon Twigger, Kei Yura, Kouichi Kimura, Tomohiro Yasuda, Tetsuo Nishikawa, Yutaka Akiyama, Chie Motono, Yuri Mukai, Hideki Nagasaki, Makiko Suwa, Paul Horton, Reiko Kikuno, Osamu Ohara, Doron Lancet, Eric Eveno, Esther Graudens, Sandrine Imbeaud, Marie Anne Debily, Yoshihide Hayashizaki, Clara Amid, Michael Han, Andreas Osanger, Toshinori Endo, Michael A. Thomas, Mika Hirakawa, Wojciech Makalowski, Mitsuteru Nakao, Nam-Soon Kim, Hyang-Sook Yoo, Sandro J. De Souza, Maria de Fatima Bonaldo, Yoshihito Niimura, Vladimir Kuryshev, Ingo Schupp, Stefan Wiemann, Matthew Bellgard, Masafumi Shionyu, Libin Jia, Danielle Thierry-Mieg, Jean Thierry-Mieg, Lukas Wagner, Qinghua Zhang, Mitiko Go, Shinsei Minoshima, Masafumi Ohtsubo, Kousuke Hanada, Peter Tonellato, Takao Isogai, Ji Zhang, Boris Lenhard, Sangsoo Kim, Zhu Chen, Ursula Hinz, Anne Estreicher, Kenta Nakai, Izabela Makalowska, Winston Hide, Nicola Tiffin, Laurens Wilming, Ranajit Chakraborty, Marcelo Bento Soares, Maria Luisa Chiusano, Yutaka Suzuki, Charles Auffray, Yumi Yamaguchi-Kabata, Takeshi Itoh, Teruyoshi Hishiki, Satoshi Fukuchi, Ken Nishikawa, Sumio Sugano, Nobuo Nomura, Yoshio Tateno, Tadashi Imanishi, Takashi Gojobori
    NUCLEIC ACIDS RESEARCH 36 D793 - D799 0305-1048 2008/01 [Refereed][Not invited]
     
    Here we report the new features and improvements in our latest release of the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/), a comprehensive annotation resource for human genes and transcripts. H-InvDB, originally developed as an integrated database of the human transcriptome based on extensive annotation of large sets of full-length cDNA (FLcDNA) clones, now provides annotation for 120 558 human mRNAs extracted from the International Nucleotide Sequence Databases (INSD), in addition to 54 978 human FLcDNAs, in the latest release H-InvDB_4.6. We mapped those human transcripts onto the human genome sequences (NCBI build 36.1) and determined 34 699 human gene clusters, which could define 34 057 (98.1%) protein-coding and 642 (1.9%) non-protein-coding loci; 858 (2.5%) transcribed loci overlapped with predicted pseudogenes. For all these transcripts and genes, we provide comprehensive annotation including gene structures, gene functions, alternative splicing variants, functional non-protein-coding RNAs, functional domains, predicted sub cellular localizations, metabolic pathways, predictions of protein 3D structure, mapping of SNPs and microsatellite repeat motifs, co-localization with orphan diseases, gene expression profiles, orthologous genes, proteinprotein interactions (PPI) and annotation for gene families. The current H-InvDB annotation resources consist of two main views: Transcript view and Locus view and eight sub-databases: the DiseaseInfo Viewer, H-ANGEL, the Clustering Viewer, G-integra, the TOPO Viewer, Evola, the PPI view and the Gene family/group.
  • Akihiro Matsuya, Ryuichi Sakate, Yoshihiro Kawahara, Kanako O. Koyanagi, Yoshiharu Sato, Yasuyuki Fujii, Chisato Yamasaki, Takuya Habara, Hajime Nakaoka, Fusano Todokoro, Kaori Yamaguchi, Toshinori Endo, Satoshi Oota, Wojciech Makalowski, Kazuho Ikeo, Yoshiyuki Suzuki, Kousuke Hanada, Katsuyuki Hashimoto, Momoki Hirai, Hisakazu Iwama, Naruya Saitou, Aiko T. Hiraki, Lihua Jin, Yayoi Kaneko, Masako Kanno, Katsuhiko Murakami, Akiko Ogura Noda, Naomi Saichi, Ryoko Sanbonmatsu, Mami Suzuki, Jun-Ichi Takeda, Masayuki Tanaka, Takashi Gojobori, Tadashi Imanishi, Takeshi Itoh
    NUCLEIC ACIDS RESEARCH 36 D787 - D792 0305-1048 2008/01 [Refereed][Not invited]
     
    Orthologs are genes in different species that evolved from a common ancestral gene by speciation. Currently, with the rapid growth of transcriptome data of various species, more reliable orthology information is prerequisite for further studies. However, detection of orthologs could be erroneous if pairwise distance-based methods, such as reciprocal BLAST searches, are utilized. Thus, as a sub-database of H-InvDB, an integrated database of annotated human genes (http://h-invitational.jp/), we constructed a fully curated database of evolutionary features of human genes, called Evola. In the process of the ortholog detection, computational analysis based on conserved genome synteny and transcript sequence similarity was followed by manual curation by researchers examining phylogenetic trees. In total, 18 968 human genes have orthologs among 11 vertebrates (chimpanzee, mouse, cow, chicken, zebrafish, etc.), either computationally detected or manually curated orthologs. Evola provides amino acid sequence alignments and phylogenetic trees of orthologs and homologs. In 'd(N)/d(S) view', natural selection on genes can be analyzed between human and other species. In 'Locus maps', all transcript variants and their exon/intron structures can be compared among orthologous gene loci. We expect the Evola to serve as a comprehensive and reliable database to be utilized in comparative analyses for obtaining new knowledge about human genes. Evola is available at http://www.h-invitational.jp/evola/.
  • Yutaka Satou, Katsuhiko Mineta, Michio Ogasawara, Yasunori Sasakura, Eiichi Shoguchi, Keisuke Ueno, Lixy Yamada, Jun Matsumoto, Jessica Wasserscheid, Ken Dewar, Graham B. Wiley, Simone L. Macmil, Bruce A. Roe, Robert W. Zeller, Kenneth E. M. Hastings, Patrick Lemaire, Erika Lindquist, Toshinori Endo, Kohji Hotta, Kazuo Inaba
    GENOME BIOLOGY 9 (10) R152  1474-760X 2008 [Refereed][Not invited]
     
    Background: The draft genome sequence of the ascidian Ciona intestinalis, along with associated gene models, has been a valuable research resource. However, recently accumulated expressed sequence tag (EST)/cDNA data have revealed numerous inconsistencies with the gene models due in part to intrinsic limitations in gene prediction programs and in part to the fragmented nature of the assembly. Results: We have prepared a less-fragmented assembly on the basis of scaffold-joining guided by paired-end EST and bacterial artificial chromosome (BAC) sequences, and BAC chromosomal in situ hybridization data. The new assembly (115.2 Mb) is similar in length to the initial assembly (116.7 Mb) but contains 1,272 (approximately 50%) fewer scaffolds. The largest scaffold in the new assembly incorporates 95 initial-assembly scaffolds. In conjunction with the new assembly, we have prepared a greatly improved global gene model set strictly correlated with the extensive currently available EST data. The total gene number (15,254) is similar to that of the initial set (15,582), but the new set includes 3,330 models at genomic sites where none were present in the initial set, and 1,779 models that represent fusions of multiple previously incomplete models. In approximately half, 5'-ends were precisely mapped using 5'-full-length ESTs, an important refinement even in otherwise unchanged models. Conclusion: Using these new resources, we identify a population of non-canonical (non-GT-AG) introns and also find that approximately 20% of Ciona genes reside in operons and that operons contain a high proportion of single-exon genes. Thus, the present dataset provides an opportunity to analyze the Ciona genome much more precisely than ever.
  • T Imanishi, T Itoh, Y Suzuki, C O'Donovan, S Fukuchi, KO Koyanagi, RA Barrero, T Tamura, Y Yamaguchi-Kabata, M Tanino, K Yura, S Miyazaki, K Ikeo, K Homma, A Kasprzyk, T Nishikawa, M Hirakawa, J Thierry-Mieg, D Thierry-Mieg, J Ashurst, LB Jia, M Nakao, MA Thomas, N Mulder, Y Karavidopoulou, LH Jin, S Kim, T Yasuda, B Lenhard, E Eveno, Y Suzuki, C Yamasaki, J Takeda, C Gough, P Hilton, Y Fujii, H Sakai, S Tanaka, C Amid, M Bellgard, MD Bonaldo, H Bono, SK Bromberg, AJ Brookes, E Bruford, P Carninci, C Chelala, C Couillault, SJ de Souza, MA Debily, MD Devignes, Dubchak, I, T Endo, A Estreicher, E Eyras, K Fukami-Kobayash, GR Gopinath, E Graudens, Y Hahn, M Han, ZG Han, K Hanada, H Hanaoka, E Harada, K Hashimoto, U Hinz, M Hirai, T Hishiki, Hopkinson, I, S Imbeaud, H Inoko, A Kanapin, Y Kaneko, T Kasukawa, J Kelso, P Kersey, R Kikuno, K Kimura, B Korn, Kuryshev, V, Makalowska, I, T Makino, S Mano, R Mariage-Samson, J Mashima, H Matsuda, HW Mewes, S Minoshima, K Nagai, H Nagasaki, N Nagata, R Nigam, O Ogasawara, O Ohara, M Ohtsubo, N Okada, T Okido, S Oota, M Ota, T Ota, T Otsuki, D Piatier-Tonneau, A Poustka, SX Ren, N Saitou, K Sakai, S Sakamoto, R Sakate, Schupp, I, F Servant, S Sherry, R Shiba, N Shimizu, M Shimoyama, AJ Simpson, B Soares, C Steward, M Suwa, M Suzuki, A Takahashi, G Tamiya, H Tanaka, T Taylor, JD Terwilliger, P Unneberg, Veeramachaneni, V, S Watanabe, L Wilming, N Yasuda, HS Yoo, M Stodolsky, W Makalowski, M Go, K Nakai, T Takagi, M Kanehisa, Y Sakaki, J Quackenbush, Y Okazaki, Y Hayashizaki, W Hide, R Chakraborty, K Nishikawa, H Sugawara, Y Tateno, Z Chen, M Oishi, P Tonellato, R Apweiler, K Okubo, L Wagner, S Wiemann, RL Strausberg, T Isogai, C Auffray, N Nomura, T Gojobori, S Sugano
    PLOS BIOLOGY 2 (6) 856 - 875 1545-7885 2004/06 [Not refereed][Not invited]
     
    The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the cDNA clones. We have developed a human gene database, called the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). It provides the following: integrative annotation of human genes, description of gene structures, details of novel alternative splicing isoforms, non-protein-coding RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein three-dimensional structure, mapping of known single nucleotide polymorphisms (SNPs), identification of polymorphic microsatellite repeats within human genes, and comparative results with mouse full-length cDNAs. The H-InvDB analysis has shown that up to 4% of the human genome sequence (National Center for Biotechnology Information build 34 assembly) may contain misassembled or missing regions. We found that 6.5% of the human gene candidates (1,377 loci) did not have a good protein-coding open reading frame, of which 296 loci are strong candidates for nonprotein-coding RNA genes. In addition, among 72,027 uniquely mapped SNPs and insertions/deletions localized within human genes, 13,215 nonsynonymous SNPs, 315 nonsense SNPs, and 452 indels occurred in coding regions. Together with 25 polymorphic microsatellite repeats present in coding regions, they may alter protein structure, causing phenotypic effects or resulting in disease. The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology.
  • T Imanishi, T Itoh, Y Suzuki, C O'Donovan, S Fukuchi, KO Koyanagi, RA Barrero, T Tamura, Y Yamaguchi-Kabata, M Tanino, K Yura, S Miyazaki, K Ikeo, K Homma, A Kasprzyk, T Nishikawa, M Hirakawa, J Thierry-Mieg, D Thierry-Mieg, J Ashurst, LB Jia, M Nakao, MA Thomas, N Mulder, Y Karavidopoulou, LH Jin, S Kim, T Yasuda, B Lenhard, E Eveno, Y Suzuki, C Yamasaki, J Takeda, C Gough, P Hilton, Y Fujii, H Sakai, S Tanaka, C Amid, M Bellgard, MD Bonaldo, H Bono, SK Bromberg, AJ Brookes, E Bruford, P Carninci, C Chelala, C Couillault, SJ de Souza, MA Debily, MD Devignes, Dubchak, I, T Endo, A Estreicher, E Eyras, K Fukami-Kobayash, GR Gopinath, E Graudens, Y Hahn, M Han, ZG Han, K Hanada, H Hanaoka, E Harada, K Hashimoto, U Hinz, M Hirai, T Hishiki, Hopkinson, I, S Imbeaud, H Inoko, A Kanapin, Y Kaneko, T Kasukawa, J Kelso, P Kersey, R Kikuno, K Kimura, B Korn, Kuryshev, V, Makalowska, I, T Makino, S Mano, R Mariage-Samson, J Mashima, H Matsuda, HW Mewes, S Minoshima, K Nagai, H Nagasaki, N Nagata, R Nigam, O Ogasawara, O Ohara, M Ohtsubo, N Okada, T Okido, S Oota, M Ota, T Ota, T Otsuki, D Piatier-Tonneau, A Poustka, SX Ren, N Saitou, K Sakai, S Sakamoto, R Sakate, Schupp, I, F Servant, S Sherry, R Shiba, N Shimizu, M Shimoyama, AJ Simpson, B Soares, C Steward, M Suwa, M Suzuki, A Takahashi, G Tamiya, H Tanaka, T Taylor, JD Terwilliger, P Unneberg, Veeramachaneni, V, S Watanabe, L Wilming, N Yasuda, HS Yoo, M Stodolsky, W Makalowski, M Go, K Nakai, T Takagi, M Kanehisa, Y Sakaki, J Quackenbush, Y Okazaki, Y Hayashizaki, W Hide, R Chakraborty, K Nishikawa, H Sugawara, Y Tateno, Z Chen, M Oishi, P Tonellato, R Apweiler, K Okubo, L Wagner, S Wiemann, RL Strausberg, T Isogai, C Auffray, N Nomura, T Gojobori, S Sugano
    PLOS BIOLOGY 2 (6) 856 - 875 1545-7885 2004/06 [Refereed][Not invited]
     
    The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the cDNA clones. We have developed a human gene database, called the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). It provides the following: integrative annotation of human genes, description of gene structures, details of novel alternative splicing isoforms, non-protein-coding RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein three-dimensional structure, mapping of known single nucleotide polymorphisms (SNPs), identification of polymorphic microsatellite repeats within human genes, and comparative results with mouse full-length cDNAs. The H-InvDB analysis has shown that up to 4% of the human genome sequence (National Center for Biotechnology Information build 34 assembly) may contain misassembled or missing regions. We found that 6.5% of the human gene candidates (1,377 loci) did not have a good protein-coding open reading frame, of which 296 loci are strong candidates for nonprotein-coding RNA genes. In addition, among 72,027 uniquely mapped SNPs and insertions/deletions localized within human genes, 13,215 nonsynonymous SNPs, 315 nonsense SNPs, and 452 indels occurred in coding regions. Together with 25 polymorphic microsatellite repeats present in coding regions, they may alter protein structure, causing phenotypic effects or resulting in disease. The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology.
  • Standardized phylogenetic tree: a tool to discover functional evolution
    J. Mol. Evol. 57 S174-181  2003 [Not refereed][Not invited]
  • Standardized phylogenetic tree: a tool to discover functional evolution
    Toshinori Endo, Soichi Ogishima, Hiroshi Tanaka
    J. Mol. Evol. 57 S174 - S181 2003 [Refereed][Not invited]
  • T Sasaki, T Matsumoto, K Yamamoto, K Sakata, T Baba, Y Katayose, JZ Wu, Y Niimura, ZK Cheng, Y Nagamura, BA Antonio, H Kanamori, S Hosokawa, M Masukawa, K Arikawa, Y Chiden, M Hayashi, M Okamoto, T Ando, H Aoki, K Arita, M Hamada, C Harada, S Hijishita, M Honda, Y Ichikawa, A Idonuma, M Iijima, M Ikeno, S Ito, T Ito, Y Ito, Y Ito, A Iwabuchi, K Kamiya, W Karasawa, S Katagiri, A Kikuta, N Kobayashi, Kono, I, K Machita, T Maehara, H Mizuno, T Mizubayashi, Y Mukai, H Nagasaki, M Nakashima, Y Nakama, Y Nakamichi, M Nakamura, N Namiki, M Negishi, Ohta, I, N Ono, S Saji, K Sakai, M Shibata, T Shimokawa, A Shomura, JY Song, Y Takazaki, K Terasawa, K Tsuji, K Waki, H Yamagata, H Yamane, S Yoshiki, R Yoshihara, K Yukawa, HS Zhong, H Iwama, T Endo, H Ito, JH Hahn, HI Kim, MY Eun, M Yano, JM Jiang, T Gojohori
    NATURE 420 (6913) 312 - 316 0028-0836 2002/11 [Not refereed][Not invited]
     
    The rice species Oryza sativa is considered to be a model plant because of its small genome size, extensive genetic map, relative ease of transformation and synteny with other cereal crops(1-4). Here we report the essentially complete sequence of chromosome 1, the longest chromosome in the rice genome. We summarize characteristics of the chromosome structure and the biological insight gained from the sequence. The analysis of 43.3 megabases (Mb) of non-overlapping sequence reveals 6,756 protein coding genes, of which 3,161 show homology to proteins of Arabidopsis thaliana, another model plant. About 30% ( 2,073) of the genes have been functionally categorized. Rice chromosome 1 is (G + C)-rich, especially in its coding regions, and is characterized by several gene families that are dispersed or arranged in tandem repeats. Comparison with a draft sequence(5) indicates the importance of a high-quality finished sequence.
  • T Sasaki, T Matsumoto, K Yamamoto, K Sakata, T Baba, Y Katayose, JZ Wu, Y Niimura, ZK Cheng, Y Nagamura, BA Antonio, H Kanamori, S Hosokawa, M Masukawa, K Arikawa, Y Chiden, M Hayashi, M Okamoto, T Ando, H Aoki, K Arita, M Hamada, C Harada, S Hijishita, M Honda, Y Ichikawa, A Idonuma, M Iijima, M Ikeno, S Ito, T Ito, Y Ito, Y Ito, A Iwabuchi, K Kamiya, W Karasawa, S Katagiri, A Kikuta, N Kobayashi, Kono, I, K Machita, T Maehara, H Mizuno, T Mizubayashi, Y Mukai, H Nagasaki, M Nakashima, Y Nakama, Y Nakamichi, M Nakamura, N Namiki, M Negishi, Ohta, I, N Ono, S Saji, K Sakai, M Shibata, T Shimokawa, A Shomura, JY Song, Y Takazaki, K Terasawa, K Tsuji, K Waki, H Yamagata, H Yamane, S Yoshiki, R Yoshihara, K Yukawa, HS Zhong, H Iwama, T Endo, H Ito, JH Hahn, HI Kim, MY Eun, M Yano, JM Jiang, T Gojohori
    NATURE 420 (6913) 312 - 316 0028-0836 2002/11 [Refereed][Not invited]
     
    The rice species Oryza sativa is considered to be a model plant because of its small genome size, extensive genetic map, relative ease of transformation and synteny with other cereal crops(1-4). Here we report the essentially complete sequence of chromosome 1, the longest chromosome in the rice genome. We summarize characteristics of the chromosome structure and the biological insight gained from the sequence. The analysis of 43.3 megabases (Mb) of non-overlapping sequence reveals 6,756 protein coding genes, of which 3,161 show homology to proteins of Arabidopsis thaliana, another model plant. About 30% ( 2,073) of the genes have been functionally categorized. Rice chromosome 1 is (G + C)-rich, especially in its coding regions, and is characterized by several gene families that are dispersed or arranged in tandem repeats. Comparison with a draft sequence(5) indicates the importance of a high-quality finished sequence.
  • T Endo, A Fedorov, SJ de Souza, W Gilbert
    MOLECULAR BIOLOGY AND EVOLUTION 19 (4) 521 - 525 0737-4038 2002/04 [Not refereed][Not invited]
     
    Are intron positions correlated with regions of high amino acid conservation? For a set of ancient conserved proteins, with intronless prokaryotic but intron-containing eukaryotic homologs, multiple sequence alignments identified residues invariant throughout evolution. Intron positions between codons show no preferences. However, introns lying after the first base of a codon prefer conserved regions, markedly in glycines. Because glycines are in excess in conserved regions, this behavior could reflect phase-one introns entering glycine residues randomly in the ancestral sequences. Examination of intron positions within codons of evolutionarily invariable amino acids showed that roughly 50% of these introns are bordered by guanines at both 5'- and 3'-ends, 25% have a G only before the intron, and 5% have a G only after the intron, whereas about 20% are bordered by nonguanine bases.
  • Do introns favor or avoid regions of amino acid conservation?
    Toshinori Endo, Alexei Fedorov, Sandro de Souza, Walter Gilbert
    Mol. Biol. Evol. 19 (4) 521 - 525 2002 [Refereed][Not invited]
  • Harukazu Suzuki, Yoshifumi Fukunishi, Ikuko Kagawa, Rintaro Saito, Hiroshi Oda, Toshinori Endo, Shinji Kondo, Hidemasa Bono, Yasushi Okazaki, Yoshihide Hayashizaki
    Genome Research 11 (10) 1758 - 1765 1088-9051 2001 [Not refereed][Not invited]
     
    We have developed a novel assay system for systematic analysis of protein-protein interactions (PPIs) that is characteristic of a PCR-mediated rapid sample preparation and a high-throughput assay system based on the mammalian two-hybrid method. Using gene-specific primers, we successfully constructed the assay samples by two rounds of PCR with up to 3.6 kb from the first-round PCR fragments. In the assay system, we designed all the steps to be performed by adding only samples, reagents, and cells into 384-well assay plates using two types of semiautomatic multiple dispensers. The system enabled us examine more than 20,000 assay wells per day. We detected 145 interactions in our pilot study using 3500 samples derived from mouse full-length enriched cDNAs. Analysis of the interaction data showed both several significant interaction clusters and predicted functions of a few uncharacterized proteins. In combination with our comprehensive mouse full-length cDNA clone bank covering a large part of the whole genes, our high-throughput assay system will discover many interactions to facilitate understanding of the function of uncharacterized proteins and the molecular mechanism of crucial biological processes, and also enable completion of a rough draft of the entire PPI panel in certain cell types or tissues of mouse within a short time.
  • Y Sugahara, P Carninci, M Itoh, K Shibata, H Konno, T Endo, M Muramatsu, Y Hayashizaki
    GENE 263 (1-2) 93 - 102 0378-1119 2001/01 [Not refereed][Not invited]
     
    To enhance the usefulness of the laboratory mouse and to facilitate the rapid assay of gene functions we have been collecting the entire set of mouse full-length cDNA by one-pass sequencing. To collect full-length cDNA clones efficiently, it is critical to construct high-quality cDNA libraries. In recent years, we have been developing a way to construct full-length cDNA libraries by using biotinylation of the cap structure (the 'CAP-trapper' method) coupled with treatment to increase reverse transcriptase efficiency at high temperature by the addition of trehalose. In this paper we report our evaluation of the quality of CAP trapper and a number of other full-length cDNA libraries, including the results of 5' end analysis of clones in CAP trapper and the other Libraries. We used a procedure that compared the 5'-ends of cDNA clones with those of genes in the public databases. Our analysis showed that 63% of cDNA clones in CAP trapper libraries had sequences that were either the same length as those of equivalent genes in the public database or 5'-extended, and that 90% of these clones maintained their coding sequences. These results indicate that the CAP trapper Library is a promising tool for collecting full-length cDNA in large-scale projects. Comparison of the quality of CAP trapper with that of other full-length-cDNA libraries confirmed the value of these libraries. (C) 2001 Elsevier Science B.V. All rights reserved.
  • Harukazu Suzuki, Yoshifumi Fukunishi, Ikuko Kagawa, Rintaro Saito, Hiroshi Oda, Toshinori Endo, Shinji Kondo, Hidemasa Bono, Yasushi Okazaki, Yoshihide Hayashizaki
    Genome Research 11 (10) 1758 - 1765 1088-9051 2001 [Refereed][Not invited]
     
    We have developed a novel assay system for systematic analysis of protein-protein interactions (PPIs) that is characteristic of a PCR-mediated rapid sample preparation and a high-throughput assay system based on the mammalian two-hybrid method. Using gene-specific primers, we successfully constructed the assay samples by two rounds of PCR with up to 3.6 kb from the first-round PCR fragments. In the assay system, we designed all the steps to be performed by adding only samples, reagents, and cells into 384-well assay plates using two types of semiautomatic multiple dispensers. The system enabled us examine more than 20,000 assay wells per day. We detected 145 interactions in our pilot study using 3500 samples derived from mouse full-length enriched cDNAs. Analysis of the interaction data showed both several significant interaction clusters and predicted functions of a few uncharacterized proteins. In combination with our comprehensive mouse full-length cDNA clone bank covering a large part of the whole genes, our high-throughput assay system will discover many interactions to facilitate understanding of the function of uncharacterized proteins and the molecular mechanism of crucial biological processes, and also enable completion of a rough draft of the entire PPI panel in certain cell types or tissues of mouse within a short time.
  • Y Sugahara, P Carninci, M Itoh, K Shibata, H Konno, T Endo, M Muramatsu, Y Hayashizaki
    GENE 263 (1-2) 93 - 102 0378-1119 2001/01 [Refereed][Not invited]
     
    To enhance the usefulness of the laboratory mouse and to facilitate the rapid assay of gene functions we have been collecting the entire set of mouse full-length cDNA by one-pass sequencing. To collect full-length cDNA clones efficiently, it is critical to construct high-quality cDNA libraries. In recent years, we have been developing a way to construct full-length cDNA libraries by using biotinylation of the cap structure (the 'CAP-trapper' method) coupled with treatment to increase reverse transcriptase efficiency at high temperature by the addition of trehalose. In this paper we report our evaluation of the quality of CAP trapper and a number of other full-length cDNA libraries, including the results of 5' end analysis of clones in CAP trapper and the other Libraries. We used a procedure that compared the 5'-ends of cDNA clones with those of genes in the public databases. Our analysis showed that 63% of cDNA clones in CAP trapper libraries had sequences that were either the same length as those of equivalent genes in the public database or 5'-extended, and that 90% of these clones maintained their coding sequences. These results indicate that the CAP trapper Library is a promising tool for collecting full-length cDNA in large-scale projects. Comparison of the quality of CAP trapper with that of other full-length-cDNA libraries confirmed the value of these libraries. (C) 2001 Elsevier Science B.V. All rights reserved.
  • Takeshi Ishimizu, Toshinori Endo, Yumi Yamaguchi-Kabata, Kazuo T. Nakamura, Fumio Sakiyama, Shigemi Norioka
    FEBS Letters 440 (3) 337 - 342 0014-5793 1998/12/04 [Refereed][Not invited]
     
    A stylar S-RNase is associated with gametophytic self-incompatibility in the Rosaceae, Solanaceae, and Scrophulariaceae. This S-RNase is responsible for S-allele-specific recognition in the self-incompatible reaction, but how it functions in specific discrimination is not clear. Window analysis of the numbers of synonymous (d(S)) and non-synonymous (d(N)) substitutions in rosaceous S-RNases detected four regions with an excess of d(N) over d(S) in which positive selection may operate (PS regions). The topology of the secondary structure of the S-RNases predicted by the PHD method is very similar to that of fungal RNase Rh whose tertiary structure is known. When the sequences of S-RNases are aligned with the sequence of RNase Rh based on the predicted secondary structures, the four PS regions correspond to two surface sites on the tertiary structure of RNase Rh. These findings suggest that in S-RNases the PS regions also form two sites and are candidates for the recognition sites for S-allele-specific discrimination. Copyright (C) 1998 Federation of European Biochemical Societies.
  • *Evolutionary significance of intra-genome duplications on human chromosomes.
    Toshinori Endo, Tadashi Imanishi, Takashi Gojobori, Hidetoshi Inoko
    Gene 210 (2) 351 - 354 1998/04 [Refereed][Not invited]
  • Toshinori Endo, Tadashi Imanishi, Takashi Gojobori, Hidetoshi Inoko
    GENE 205 (1/2) 19 - 27 1997/12 [Refereed][Not invited]
  • Yoshio Tateno, Kazuho Ikeo, Hidemi Watanabe, Toshinori Endo, Yumi Yamaguchi, Yoshiyuki Suzuki, Kazunari Takahashi, Kazuhisa Tsunoyama, Masahiko Kawai, Yuichi Kawanishi, Kimihiko. Naito, Takashi Gojobori
    J. Mol Evol. 44(Suppl 1) S38 - S43 1997/01 [Refereed][Not invited]
  • Akishinonomiya Fumihito, Tetsuo Miyake, Masaru Takada, Ryosuke Shingu, Toshinori Endo, Takashi Gojobori, Norio Kondo, Susumu Ohno
    Proc. of Natl. Acad. Sci., USA. 93 (13) 6792 - 6795 1996/06 [Refereed][Not invited]
  • T Endo, K Ikeo, T Gojobori
    MOLECULAR BIOLOGY AND EVOLUTION 13 (5) 685 - 690 0737-4038 1996/05 [Refereed][Not invited]
     
    We conducted a systematic search for the candidate genes on which positive selection may operate, on the premise that for such genes the number of nonsynonymous substitution is expected to be larger than that of synonymous substitutions when the nucleotide sequences of the genes under investigation are compared with each other. By obtaining 3,595 groups of homologous sequences from the DDBJ, EMBL, and GenBank DNA sequence databases, we found that 17 gene groups can be the candidates for the genes on which positive selection may operate. Thus, such genes are found to occupy only about 0.5% of the vast number of gene groups so far available. Interestingly enough, 9 out of the 17 gene groups were the surface antigens of parasites or viruses.
  • Large-scale search for genes on which positive selection may operate.
    Toshinori Endo
    Dessertation. Graduate School for Advanced Studies 1995 [Refereed][Not invited]
  • VC FOLETTA, MH SONOBE, T SUZUKI, T ENDO, H IBA, DR COHEN
    ONCOGENE 9 (11) 3305 - 3311 0950-9232 1994/11 [Refereed][Not invited]
     
    Transcription factor AP-1 is comprised of multiple protein complexes that include members of a family of genes related to the proto-oncogene c-fos. In this report, we have extended the analysis of one member of this family, fos-related antigen-2 (fra-2), by isolating and characterising genomic and cDNA clones encoding:the mouse fra-2 homolog. The overall gene structure (number and positions of introns) was similar to that of both the chicken fra-2 gene and other members of the fos family, and the relative positions of putative enhancers in the 5' regulatory region were well conserved between the mouse and chicken fra-2 genes. High levels of fra-2 mRNA were detected in ovary, stomach, small and large intestine, brain, lung and heart. The mouse Fra-2 protein showed 94% and 87.5% conservation with human and chicken Fra-2, respectively, and mouse Fra-2, like the chicken homolog, induced transformation of chicken embryo fibroblasts. The characterisation of the mouse fra-2 gene provides a basis for analysis of Fra-2 function in the whole animal.
  • Takashi Kameda, Atsuko Akahori, Martha H. Sonobe, Takehisa Suzuki, Toshinori Endo, Hideo Iba
    Proc. Natl Acad of Sci., U.S.A. 90 (20) 9369 - 9373 1993/10 [Refereed][Not invited]
  • T SUZUKI, H OKUNO, T YOSHIDA, T ENDO, H NISHINA, H IBA
    NUCLEIC ACIDS RESEARCH 19 (20) 5537 - 5542 0305-1048 1991/10 [Refereed][Not invited]
     
    Fra-2, one of the Fos-related antigens, is promptly expressed after the growth stimulation of fibroblasts, but its induction peak is later than that of c-Fos. In this report, we examined biochemical properties of Fra-2 and compared them with those of two other Fos family proteins, c-Fos and Fra-1. Like c-Fos and Fra-1, Fra-2 formed stable heterodimers with c-Jun, JunB or JunD in vitro and all these complexes had specific DNA-binding activity to AP-1-binding sites (AP-1 sites) or related sequences. When transiently introduced into a mouse embryonic carcinoma cell line, F9, with reporter genes containing the AP-1 site from the collagenase gene, fra-2 plus c-jun suppressed the transactivation by c-jun alone. This property of Fra-2 is in clear contrast to that of c-Fos, which stimulates the transcriptional activity of c-Jun by forming a stable heterodimer. Analysis of chimeric proteins between c-Fos and Fra-2 indicated that this difference is mainly attributable to their C terminal-half regions. Interestingly, this suppressive effect of Fra-2 was not observed in the combination with JunD: fra-2 plus junD, like c-fos plus junD, had higher transcriptional activity than junD alone. Fra-1 showed essentially the same transcriptional regulatory properties as Fra-2. These differential properties greatly expand the potential range of regulatory functions of the Fos family proteins.

Books etc

  • 分子生物学者のためのバイオインフォマティクス入門
    共立出版 2001
  • Tracing Biological Evolution in Protein and Gene Structures.
    Takashi Gojobori, Toshinori Endo, Kazuho Ikeo (Joint workMitiko Go and Paul Schimmel eds. pp. 249-260)
    Elsevier Science B. V., Amsterdam. 1995

Conference Activities & Talks

  • Evolutionary analysis of Mollicutes based on standardized phylogenetic tree  [Not invited]
    遠藤 俊徳
    2013/10

MISC

  • Yutaka Satou, Katsuhiko Mineta, Michio Ogasawara, Yasunori Sasakura, Eiichi Shoguchi, Keisuke Ueno, Lixy Yamada, Jun Matsumoto, Jessica Wasserscheid, Ken Dewar, Graham B. Wiley, Simone L. Macmil, Bruce A. Roe, Robert W. Zeller, Kenneth E. M. Hastings, Patrick Lemaire, Erika Lindquist, Toshinori Endo, Kohji Hotta, Kazuo Inaba  GENOME BIOLOGY  9-  (10)  R152  2008  [Not refereed][Not invited]
     
    Background: The draft genome sequence of the ascidian Ciona intestinalis, along with associated gene models, has been a valuable research resource. However, recently accumulated expressed sequence tag (EST)/cDNA data have revealed numerous inconsistencies with the gene models due in part to intrinsic limitations in gene prediction programs and in part to the fragmented nature of the assembly. Results: We have prepared a less-fragmented assembly on the basis of scaffold-joining guided by paired-end EST and bacterial artificial chromosome (BAC) sequences, and BAC chromosomal in situ hybridization data. The new assembly (115.2 Mb) is similar in length to the initial assembly (116.7 Mb) but contains 1,272 (approximately 50%) fewer scaffolds. The largest scaffold in the new assembly incorporates 95 initial-assembly scaffolds. In conjunction with the new assembly, we have prepared a greatly improved global gene model set strictly correlated with the extensive currently available EST data. The total gene number (15,254) is similar to that of the initial set (15,582), but the new set includes 3,330 models at genomic sites where none were present in the initial set, and 1,779 models that represent fusions of multiple previously incomplete models. In approximately half, 5'-ends were precisely mapped using 5'-full-length ESTs, an important refinement even in otherwise unchanged models. Conclusion: Using these new resources, we identify a population of non-canonical (non-GT-AG) introns and also find that approximately 20% of Ciona genes reside in operons and that operons contain a high proportion of single-exon genes. Thus, the present dataset provides an opportunity to analyze the Ciona genome much more precisely than ever.

Research Grants & Projects

  • GPGPUによる高速マルチプルアラインメント法の開発
    Date (from‐to) : 2008
  • Development of GPGPU based multiple sequence alignment method
    Date (from‐to) : 2008
  • オーファンエンザイム情報に基づく遺伝子機能予測
    ゲノム関係研究
    Date (from‐to) : 2005
  • Prediction of the function of hypothetical genes based on the information of orphan enzymes
    0104 (Japanese Only)
    Date (from‐to) : 2005

Educational Activities

Teaching Experience

  • 卒業論文
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
  • Advanced Exercise in Bioengineering and Bioinformatics
    開講年度 : 2020
    課程区分 : 修士課程
    開講学部 : 情報科学院
  • Advanced Exercise in Bioengineering and Bioinformatics
    開講年度 : 2020
    課程区分 : 修士課程
    開講学部 : 情報科学研究科
  • Information Biology
    開講年度 : 2020
    課程区分 : 修士課程
    開講学部 : 情報科学研究科
    キーワード : バイオインフォマティクス、ゲノムとプロテオーム、分子進化、遺伝子発現、生物学データベース
  • Information Biology
    開講年度 : 2020
    課程区分 : 修士課程
    開講学部 : 情報科学院
    キーワード : バイオインフォマティクス、ゲノムとプロテオーム、分子進化、遺伝子発現、生物学データベース
  • Bioengineering
    開講年度 : 2020
    課程区分 : 修士課程
    開講学部 : 情報科学研究科
    キーワード : 遺伝情報, genetic information, バイオインフォマティクス, bioinformatics, イメージング, imaging, 生体医工学, biomedical engineering, 細胞力学, cell mechanics
  • Advanced Study in Bioengineering and Bioinformatics
    開講年度 : 2020
    課程区分 : 博士後期課程
    開講学部 : 情報科学院
  • Advamced Study in Bioengineering and Bioinformatics
    開講年度 : 2020
    課程区分 : 博士後期課程
    開講学部 : 情報科学研究科
  • Information Biology
    開講年度 : 2020
    課程区分 : 博士後期課程
    開講学部 : 情報科学研究科
    キーワード : バイオインフォマティクス、ゲノムとプロテオーム、分子進化、遺伝子発現、生物学データベース
  • Information Biology
    開講年度 : 2020
    課程区分 : 博士後期課程
    開講学部 : 情報科学院
    キーワード : バイオインフォマティクス、ゲノムとプロテオーム、分子進化、遺伝子発現、生物学データベース
  • Bioengineering
    開講年度 : 2020
    課程区分 : 博士後期課程
    開講学部 : 情報科学研究科
    キーワード : 遺伝情報, genetic information, バイオインフォマティクス, bioinformatics, イメージング, imaging, 生体医工学, biomedical engineering, 細胞力学, cell mechanics
  • Introduction to Electronics and Information Engineering
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 情報理論, 計算機ハードウエア, 電子デバイス, 生体情報, 生命科学, 電子回路, 通信, メディア, ネットワーク, 電気回路, 制御工学
  • Internship Ⅰ
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 創造的人材育成、実践的人材育成、就業体験、国内外インターンシップ
  • Internship Ⅱ
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 創造的人材育成、実践的人材育成、就業体験、国内外インターンシップ
  • Biology I
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 全学教育
    キーワード : 生体高分子,細胞の構造と機能,エネルギー代謝,細胞の成長と分裂,遺伝現象と遺伝子発現制御
  • Cell biology
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 細胞膜、膜タンパク質、膜輸送、イオンチャネル、膜の電気的特性、細胞内区画、タンパク質輸送、シグナルトランスダクション、細胞のメッセンジャー、アポトーシス
  • Exercise in Technical English
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 英語読解力,英語作文力,科学技術英語表現
  • Exercise in Bioengineering and Bioinformatics Ⅱ
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 数値計算法,シミュレーション,統計と検定,データベース,情報検索
  • Exercise in Bioengineering and Bioinformatics Ⅰ
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 電磁気学,線形システム論,信号処理,電気・電子・ディジタル回路
  • Bioengineering and Bioinformatics Laboratory Ⅱ
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 遺伝情報,生体電気現象,生体機能情報,生体計測,バイオエレクトロニクス
  • Bioengineering and Bioinformatics Laboratory Ⅰ
    開講年度 : 2020
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 遺伝情報,物理計測とセンサ,電気・電子計測,ディジタル技術,力学運動

Committee Membership

  • 2011   Society for Molecular Biology and Evolution   Annual meeting committee member   Society for Molecular Biology and Evolution


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