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Takako S. Chikenji
Faculty of Health Sciences　Health Sciences　Department of Rehabilitation Science
Professor
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Last Updated :2024/05/10

Researcher Profile and Settings

Affiliation

  • Faculty of Health Sciences　Health Sciences　Department of Rehabilitation Science

Job Title

  • Professor

Degree

  • Ph.D

URL

Research funding number

  • 40452982

ORCID ID

J-Global ID

Research Interests

  • 組織再生   細胞老化   慢性炎症   再生医療   自己免疫疾患   慢性腎臓病   間葉系幹細胞   組織線維化   

Research Areas

  • Life sciences / Nutrition and health science
  • Life sciences / Anatomy
  • Life sciences / Orthopedics
  • Life sciences / Rehabilitation science

Educational Organization

Academic & Professional Experience

  • 2021/08 - Today 札幌医科大学医学部解剖学第二講座 客員教授
  • 2021/04 - Today Hokkaido University Faculty of Health Sciences
  • 2019 - 2021/07 札幌医科大学医学部解剖学第２講座 客員准教授
  • 2019/04 - 2021/03 Hokkaido University Associate professor
  • 2018/07 - 2019/06 Mayo Clinic Research fellow
  • 2014/10 - 2018/07 Sapporo Medical University School of Medicine Department of Anatomy Assistant professor
  • 2013/04 - 2014/09 Sapporo Medical University School of Health Sciences
  • 2010 - 2012 Mayo Clinic Research Fellow

Association Memberships

  • 日本ハンドセラピィ学会   JAPANESE ASSOCIATION OF OCCUPATIONAL THERAPISTS   JAPANESE SOCIETY FOR SURGERY OF THE HAND   THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE   International Cell Senescence Association   Orthopedic Research Society   日本炎症再生学会   

Research Activities

Published Papers

  • Arisa Kita, Sena Yamamoto, Yuki Saito, Takako S. Chikenji
    Frontiers in Physiology 15 2024/02/19 
    Cellular senescence is a biological mechanism that prevents abnormal cell proliferation during tissue repair, and it is often accompanied by the secretion of various factors, such as cytokines and chemokines, known as the senescence-associated secretory phenotype (SASP). SASP-mediated cell-to-cell communication promotes tissue repair, regeneration, and development. However, senescent cells can accumulate abnormally at injury sites, leading to excessive inflammation, tissue dysfunction, and intractable wounds. The effects of cellular senescence on skin wound healing can be both beneficial and detrimental, depending on the condition. Here, we reviewed the functional differences in cellular senescence that emerge during wound healing, chronic inflammation, and skin aging. We also review the latest mechanisms of wound healing in the epidermis, dermis, and subcutaneous fat, with a focus on cellular senescence, chronic inflammation, and tissue regeneration. Finally, we discuss the potential clinical applications of promoting and inhibiting cellular senescence to maximize benefits and minimize detrimental effects.
  • Shogo Ijima, Yuki Saito, Sena Yamamoto, Kentaro Nagaoka, Taiki Iwamoto, Arisa Kita, Maki Miyajima, Tsukasa Sato, Akihiro Miyazaki, Takako S Chikenji
    Immunity & ageing : I & A 20 (1) 72 - 72 2023/12/05 [Refereed][Not invited]
     
    Oral lichen planus is a chronic inflammatory condition that adversely affects the oral mucosa; however, its etiology remains elusive. Consequently, therapeutic interventions for oral lichen planus are limited to symptomatic management. This study provides evidence of the accumulation of senescent mesenchymal cells, CD8 + T cells, and natural killer cells in patients with oral lichen planus. We profiled the patients' tissues using the National Center for Biotechnology Information Gene Expression Omnibus database and found that senescence-related genes were upregulated in these tissues by gene set enrichment analysis. Immunohistochemical analysis showed increased senescent mesenchymal cells in the subepithelial layer of patients with oral lichen planus. Single-cell RNA-seq data retrieved from the Gene Expression Omnibus database of patients with oral lichen planus revealed that mesenchymal cells were marked by the upregulation of senescence-related genes. Cell-cell communication analysis using CellChat showed that senescent mesenchymal cells significantly influenced CD8 + T cells and natural killer cells via CXCL12-CXCR4 signaling, which is known to activate and recruit CD8 + T cells and NK cells. Finally, in vitro assays demonstrated that the secretion of senescence-associated factors from mesenchymal cells stimulated the activation of T cells and natural killer cells and promoted epithelial cell senescence and cytotoxicity. These findings suggest that the accumulation of mesenchymal cells with senescence-associated secretory phenotype may be a key driver of oral lichen planus pathogenesis.
  • 千見寺貴子, 齋藤悠城
    医学のあゆみ 医歯薬出版(株) 287 (5) 321 - 326 0039-2359 2023/11 
    細胞老化はさまざまな臓器における慢性炎症や線維化,さらには個体老化との関連性が報告されたことから,健康寿命を延伸する治療ターゲットとして注目されている.一方で,細胞老化はがん抑制機構として知られるだけでなく,胚の発生,創傷治癒さらには組織幹細胞の活性化を介した組織再生や,リプログラミングに至るまで幅広い生物学的プロセスに関与する,生体にとって有益な役割も担っていることが明らかになっている.そのため,"光"と"影"を含めた細胞老化の多面的な理解が,安全で有効な老化細胞をターゲットとした疾病予防・治療の確立に向けて重要な課題と考えられている.本稿では,細胞老化の多様な役割のなかで,特に細胞老化の有益な働きを中心に,さまざまな枠組みにおける細胞老化の役割について紹介する.(著者抄録)
  • 齋藤 悠城, 千見寺 貴子
    基礎理学療法学 (一社)日本基礎理学療法学会 25 (1) 61 - 68 2022/10 
    細胞老化はがん抑制や個体発生,さらには組織再生など生体に重要な機能を果たす一方で,加齢や慢性炎症などを進展する二面性が報告されている。近年,骨格筋への運動ストレスが細胞老化を誘導して骨格筋の修復や再生を促進するだけでなく,慢性炎症や線維化など骨格筋病態へも関与することが明らかになってきた。細胞老化制御機構の解明は新たな骨格筋治療の突破口になる可能性を秘めている。本稿では,運動による骨格筋の細胞老化と組織修復プロセスを中心に,骨格筋における細胞老化の影響について概説する。(著者抄録)
  • 骨格筋における細胞老化と筋再生
    千見寺 貴子, 齋藤 悠城
    基礎老化研究 日本基礎老化学会 46 (3) 8 - 8 0912-8921 2022/10
  • Yuki Saito, Maki Miyajima, Sena Yamamoto, Norihiro Miura, Tsukasa Sato, Arisa Kita, Shogo Ijima, Mineko Fujimiya, Takako S Chikenji
    Stem Cells Translational Medicine 2157-6564 2022/04/25 [Refereed]
     
    Abstract Up to 60% of patients with systemic lupus erythematosus (SLE) experience autonomic symptom. Sympathetic nervous system damage can cause dysfunction of the bone marrow that activates inflammatory cells, potentially causing multiple organ damage. We hypothesized that sympathetic nervous system damage would induce bone marrow dysfunction with multiple organ damage in SLE, and that multiple organ damage could be improved by therapy targeting the nervous system. Here, we showed that damage to autonomic nerves and Schwann cells occurred in the bone marrow and central nervous system of SLE model mice. A neurotoxic drug increased mortality and induced severe neuropathy and multiple organ damage, while a neuroprotective drug prevented multiple organ damage. The administration of bone marrow-derived mesenchymal stromal cells (BMSCs) cultured on a 3-dimensional fiber scaffold improved bone marrow neuropathy, skin lesions, kidney function, and mortality. Our results reveal that bone marrow neuropathy influence multiple organ damage associated with SLE, and improvement of bone marrow neuropathy by intrathecal injection of BMSC may be a target for SLE multiple-organ damage.
  • Arisa Kita, Yuki Saito, Norihiro Miura, Maki Miyajima, Sena Yamamoto, Tsukasa Sato, Takatoshi Yotsuyanagi, Mineko Fujimiya, Takako S. Chikenji
    Communications Biology 5 (1) 2022/04 [Refereed]
     
    Abstract Pathologic diabetic wound healing is caused by sequential and progressive deterioration of hemostasis, inflammation, proliferation, and resolution/remodeling. Cellular senescence promotes wound healing; however, diabetic wounds exhibit low levels of senescent factors and accumulate senescent cells, which impair the healing process. Here we show that the number of p15INK4B + PDGFRα + senescent mesenchymal cells in adipose tissue increases transiently during early phases of wound healing in both non-diabetic mice and humans. Transplantation of adipose tissue from diabetic mice into non-diabetic mice results in impaired wound healing and an altered cellular senescence–associated secretory phenotype (SASP), suggesting that insufficient induction of adipose tissue senescence after injury is a pathological mechanism of diabetic wound healing. These results provide insight into how regulation of senescence in adipose tissue contributes to wound healing and could constitute a basis for developing therapeutic treatment for wound healing impairment in diabetes.
  • Shogo Ijima, Yuki Saito, Kentaro Nagaoka, Sena Yamamoto, Tsukasa Sato, Norihiro Miura, Taiki Iwamoto, Maki Miyajima, Takako S Chikenji
    Frontiers in immunology 13 960601 - 960601 2022 
    Systemic lupus erythematosus (SLE) is a chronic autoimmune inflammatory disease characterized by the involvement of multiple organs. Lupus nephritis (LN) is a major risk factor for overall morbidity and mortality in SLE patients. Hence, designing effective drugs is pivotal for treating individuals with LN. Fisetin plays a senolytic role by specifically eliminating senescent cells, inhibiting cell proliferation, and exerting anti-inflammatory, anti-oxidant, and anti-tumorigenic effects. However, limited research has been conducted on the utility and therapeutic mechanisms of fisetin in chronic inflammation. Similarly, whether the effects of fisetin depend on cell type remains unclear. In this study, we found that LN-prone MRL/lpr mice demonstrated accumulation of Ki-67-positive myofibroblasts and p15INK4B-positive senescent tubular epithelial cells (TECs) that highly expressed transforming growth factor β (TGF-β). TGF-β stimulation induced senescence of NRK-52E renal TECs and proliferation of NRK-49F renal fibroblasts, suggesting that TGF-β promotes senescence and proliferation in a cell type-dependent manner, which is inhibited by fisetin treatment in vitro. Furthermore, fisetin treatment in vivo reduced the number of senescent TECs and myofibroblasts, which attenuated kidney fibrosis, reduced senescence-associated secretory phenotype (SASP) expression, and increased TEC proliferation. These data suggest that the effects of fisetin vary depending on the cell type and may have therapeutic effects in complex and diverse LN pathologies.
  • Yuki Saito, Maki Miyajima, Sena Yamamoto, Tsukasa Sato, Norihiro Miura, Mineko Fujimiya, Takako S. Chikenji
    Frontiers in Immunology 12 692321 - 692321 2021/11/03 [Refereed][Not invited]
     
    Neuropsychiatric manifestations targeting the central, peripheral, and autonomic nervous system are common in systemic lupus erythematosus (SLE); collectively, these symptoms are termed neuropsychiatric SLE (NPSLE). Among a wide variety of neuropsychiatric symptoms, depression is observed in about 24-39% of SLE patients. Several cytokines and chemokines have been identified as biomarkers or therapeutic targets of NPSLE; in particular, the levels of type 1 interferons, TNFs, and IL-6 are elevated in SLE patient’s cerebrospinal fluid (CSF), and these factors contribute to the pathology of depression. Here, we show that senescent neural cells accumulate in the hippocampal cornu ammonis 3 (CA3) region in MRL/lpr SLE model mice with depressive behavior. Furthermore, oral administration of fisetin, a senolytic drug, reduced the number of senescent neural cells and reduced depressive behavior in the MRL/lpr mice. In addition, transcription of several senescence and senescence-associated secretory phenotype (SASP) factors in the hippocampal region also decreased after fisetin treatment in the MRL/lpr mice. These results indicate that the accumulation of senescent neural cells in the hippocampus plays a role in NPSLE pathogenesis, and therapies targeting senescent cells may represent a candidate approach to treat NPSLE.
  • U-39最先端研究紹介 骨格筋間葉系前駆細胞の細胞老化と骨格筋の再生
    齋藤 悠城, 藤宮 峯子, 千見寺 貴子
    日本基礎理学療法学会学術大会プログラム・抄録集 (一社)日本基礎理学療法学会 26回 シンポジウム2 - シンポジウム2 2021/10
  • Yuki Saito, Takako S. Chikenji
    Frontiers in Pharmacology 12 739510 - 739510 2021/09/06 [Refereed][Invited]
     
    Skeletal muscle undergoes vigorous tissue remodeling after injury. However, aging, chronic inflammatory diseases, sarcopenia, and neuromuscular disorders cause muscle loss and degeneration, resulting in muscular dysfunction. Cellular senescence, a state of irreversible cell cycle arrest, acts during normal embryonic development and remodeling after tissue damage; when these processes are complete, the senescent cells are eliminated. However, the accumulation of senescent cells is a hallmark of aging tissues or pathological contexts and may lead to progressive tissue degeneration. The mechanisms responsible for the effects of senescent cells have not been fully elucidated. Here, we review current knowledge about the beneficial and detrimental effects of senescent cells in tissue repair, regeneration, aging, and age-related disease, especially in skeletal muscle. We also discuss how senescence of muscle stem cells and muscle-resident fibro-adipogenic progenitors affects muscle pathologies or regeneration, and consider the possibility that immunosenescence leads to muscle pathogenesis. Finally, we explore senotherapy, the therapeutic targeting of senescence to treat age-related disease, from the standpoint of improving muscle regeneration.
  • Masako Nakano, Kenta Kubota, Shin Hashizume, Eiji Kobayashi, Takako S. Chikenji, Yuki Saito, Mineko Fujimiya
    Brain, Behavior, & Immunity - Health 9 100149 - 100149 2666-3546 2020/12
  • Koichi Himori, Yuki Ashida, Daisuke Tatebayashi, Masami Abe, Yuki Saito, Takako Chikenji, Håkan Westerblad, Daniel C Andersson, Takashi Yamada
    Arthritis & rheumatology (Hoboken, N.J.) 73 (5) 848 - 857 2020/11/15 [Refereed][Not invited]
     
    OBJECTIVE: High-force eccentric contractions (ECCs) have traditionally been excluded from rehabilitation programs of patients with idiopathic inflammatory myopathies (IIMs) due to unverified fear of causing muscle damage and inflammation. Here, we used an IIM animal model, experimental autoimmune myositis (EAM) mice, to investigated whether ECC training can be safely and effectively used to counteract muscle weakness in IIM. METHODS: EAM was induced in Balb/c mice by immunization with three injections of myosin emulsified in complete Freund's adjuvant. Control (CNT: n=12) and EAM (n=12) mice were exposed to an acute bout of 100 ECCs or 4 weeks of ECC training (20 ECCs every other day). To induce ECCs, plantar flexor muscles were electrically stimulated while the ankle was forcibly dorsiflexed. RESULTS: Less cell damage, as assessed by Evans blue dye uptake, was observed in EAM than in CNT muscles after an acute bout of 100 ECCs (P < 0.05). Maximum Ca2+ -activated force was decreased in skinned gastrocnemius muscle fibers from EAM mice and this was accompanied by increased expression of the endoplasmic reticulum (ER) stress proteins glucose-regulated protein 78 and 94 (P < 0.05). ECC training prevented the force decrease and the increase in ER stress proteins, and also enhanced the expression and myofibrillar binding of small heat shock proteins (sHSP) (P < 0.05), which can stabilize myofibrillar structure and function. CONCLUSION: ECC training protected against the reduction in myofibrillar force generating capacity in an IIM mouse model and this occurred via inhibition of ER stress responses and sHSP-mediated myofibrillar stabilization.
  • Masako Nakano, Kenta Kubota, Eiji Kobayashi, Takako S Chikenji, Yuki Saito, Naoto Konari, Mineko Fujimiya
    Scientific reports 10 (1) 10772 - 10772 2020/07/01 [Refereed][Not invited]
     
    Alzheimer's disease (AD) is characterized by the accumulation of amyloid-β and tau. We previously reported that administration of bone marrow mesenchymal stem cells (BM-MSCs) ameliorates diabetes-induced cognitive impairment by transferring exosomes derived from these cells into astrocytes. Here, we show that intracerebroventricularly injected BM-MSCs improve cognitive impairment in AD model mice by ameliorating astrocytic inflammation as well as synaptogenesis. Although AD model mice showed an increase in NF-κB in the hippocampus, BM-MSC-treated AD model mice did not show this increase but showed an increase in levels of microRNA (miR)-146a in the hippocampus. Intracerebroventricularly injected BM-MSCs were attached to the choroid plexus in the lateral ventricle, and thus, BM-MSCs may secrete exosomes into the cerebrospinal fluid. In vitro experiments showed that exosomal miR-146a secreted from BM-MSCs was taken up into astrocytes, and an increased level of miR-146a and a decreased level of NF-κB were observed in astrocytes. Astrocytes are key cells for the formation of synapses, and thus, restoration of astrocytic function may have led to synaptogenesis and correction of cognitive impairment. The present study indicates that exosomal transfer of miR-146a is involved in the correction of cognitive impairment in AD model mice.
  • Yuki Saito, Takako S. Chikenji, Takashi Matsumura, Masako Nakano, Mineko Fujimiya
    Nature Communications 2020/02 [Refereed][Not invited]
  • Yuki Saito, Takako S. Chikenji, Yuichi Takata, Tomoaki Kamiya, Eiichi Uchiyama
    BMC Musculoskeletal Disorders 1471-2474 2019/12 [Refereed][Not invited]
  • Takako S. Chikenji, Yuki Saito, Naoto Konari, Masako Nakano, Yuka Mizue, Miho Otani, Mineko Fujimiya
    EBioMedicine Elsevier {BV} 44 86 - 97 2352-3964 2019/06 [Refereed][Not invited]
  • Takashi Matsumura, Yuki Saito, Tomoyuki Suzuki, Atsushi Teramoto, Yasuhiro Ozasa, Toshihiko Yamashita, Mineko Fujimiya, Takako S. Chikenji
    Frontiers in Immunology 10 241  1664-3224 2019/02 [Refereed][Not invited]
  • Akira Saito, Kanna Nagaishi, Kousuke Iba, Yuka Mizue, Takako Chikenji, Miho Otani, Masako Nakano, Kazusa Oyama, Toshihiko Yamashita, Mineko Fujimiya
    Scientific Reports 8 (1) 2045-2322 2018/12/01 [Refereed][Not invited]
     
    Bone marrow-derived mesenchymal stem cells (BM-MSCs) are the most valuable source of autologous cells for transplantation and tissue regeneration to treat osteoporosis. Although BM-MSCs are the primary cells responsible for maintaining bone metabolism and homeostasis, their regenerative ability may be attenuated in postmenopausal osteoporosis patients. Therefore, we first examined potential abnormalities of BM-MSCs in an oestrogen-deficient rat model constructed by ovariectomy (OVX-MSCs). Cell proliferation, mobilisation, and regulation of osteoclasts were downregulated in OVX-MSCs. Moreover, therapeutic effects of OVX-MSCs were decreased in OVX rats. Accordingly, we developed a new activator for BM-MSCs using human umbilical cord extracts, Wharton's jelly extract supernatant (WJS), which improved cell proliferation, mobilisation and suppressive effects on activated osteoclasts in OVX-MSCs. Bone volume, RANK and TRACP expression of osteoclasts, as well as proinflammatory cytokine expression in bone tissues, were ameliorated by OVX-MSCs activated with WJS (OVX-MSCs-WJ) in OVX rats. Fusion and bone resorption activity of osteoclasts were suppressed in macrophage-induced and primary mouse bone marrow cell-induced osteoclasts via suppression of osteoclast-specific genes, such as Nfatc1, Clcn7, Atp6i and Dc-stamp, by co-culture with OVX-MSCs-WJ in vitro. In this study, we developed a new activator, WJS, which improved the functional abnormalities and therapeutic effects of BM-MSCs on postmenopausal osteoporosis.
  • 作業療法疾患別ガイドライン 認知症
    竹原 敦, 小川 敬之, 上城 憲司, 高木 雅之, 田平 隆行, 千見寺 貴子, 西浦 裕子, 西田 征治, 安田 大典, 山口 智晴, 磯 ふみ子, 市川 誠, 井上 忠俊, 木下 遥, 高坂 駿, 越當 美智子, 近藤 真知子, 坂上 真理, 坂本 千晶, 渋谷 晋太郎, 下田 佳央莉, 菅沼 一平, 仙波 梨沙, 田中 純子, 爲近 岳夫, 土谷 里織, 新中 浩司, 藤岡 崇, 古井 香苗, 松尾 崇史, 松尾 涼太, 村井 達彦, 山田 真季, 米澤 武人
    作業療法 (一社)日本作業療法士協会 37 (1) 3 - 11 0289-4920 2018/02 [Not refereed][Not invited]
  • Eiji Kobayashi, Masako Nakano, Kenta Kubota, Nobuaki Himuro, Shougo Mizoguchi, Takako Chikenji, Miho Otani, Yuka Mizue, Kanna Nagaishi, Mineko Fujimiya
    Scientific reports 8 (1) 1712 - 1712 2018/01/26 [Refereed][Not invited]
     
    Although the cognitive impairment in Alzheimer's disease (AD) is believed to be caused by amyloid-β (Aβ) plaques and neurofibrillary tangles (NFTs), several postmortem studies have reported cognitive normal subjects with AD brain pathology. As the mechanism underlying these discrepancies has not been clarified, we focused the neuroprotective role of astrocytes. After examining 47 donated brains, we classified brains into 3 groups, no AD pathology with no dementia (N-N), AD pathology with no dementia (AD-N), and AD pathology with dementia (AD-D), which represented 41%, 21%, and 38% of brains, respectively. No differences were found in the accumulation of Aβ plaques or NFTs in the entorhinal cortex (EC) between AD-N and AD-D. Number of neurons and synaptic density were increased in AD-N compared to those in AD-D. The astrocytes in AD-N possessed longer or thicker processes, while those in AD-D possessed shorter or thinner processes in layer I/II of the EC. Astrocytes in all layers of the EC in AD-N showed enhanced GLT-1 expression in comparison to those in AD-D. Therefore these activated forms of astrocytes with increased GLT-1 expression may exert beneficial roles in preserving cognitive function, even in the presence of Aβ and NFTs.
  • Kenta Kubota, Masako Nakano, Eiji Kobayashi, Yuka Mizue, Takako Chikenji, Miho Otani, Kanna Nagaishi, Mineko Fujimiya
    PloS one 13 (9) e0204252  2018 [Refereed][Not invited]
     
    Increasing evidence suggests that an enriched environment (EE) ameliorates cognitive impairment by promoting repair of brain damage. However, the mechanisms by which this occurs have not been determined. To address this issue, we investigated whether an EE enhanced the capability of endogenous bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs) to prevent hippocampal damage due to diabetes by focusing on miRNA carried in BM-MSC-derived exosomes. In diabetic streptozotocin (STZ) rats housed in an EE (STZ/EE), cognitive impairment was significantly reduced, and both neuronal and astroglial damage in the hippocampus was alleviated compared with STZ rats housed in conventional cages (STZ/CC). BM-MSCs isolated from STZ/CC rats had functional and morphological abnormalities that were not detected in STZ/EE BM-MSCs. The miR-146a levels in exosomes in conditioned medium of cultured BM-MSCs and serum from STZ/CC rats were decreased compared with non-diabetic rats, and the level was restored in STZ/EE rats. Thus, the data suggest that increased levels of miR-146a in sera were derived from endogenous BM-MSCs in STZ/EE rats. To examine the possibility that increased miR-146a in serum may exert anti-inflammatory effects on astrocytes in diabetic rats, astrocytes transfected with miR-146a were stimulated with advanced glycation end products (AGEs) to mimic diabetic conditions. The expression of IRAK1, NF-κB, and tumor necrosis factor-α was significantly higher in AGE-stimulated astrocytes, and these factors were decreased in miR-146a-transfected astrocytes. These results suggested that EEs stimulate up-regulation of exosomal miR-146a secretion by endogenous BM-MSCs, which exerts anti-inflammatory effects on damaged astrocytes and prevents diabetes-induced cognitive impairment.
  • Yuki Saito, Takako Chikenji, Yasuhiro Ozasa, Mineko Fujimiya, Toshihiko Yamashita, Anne Gingery, Kousuke Iba
    Scientific Reports 7 (1) 2017/12 [Refereed][Not invited]
  • Kanna Nagaishi, Yuka Mizue, Takako Chikenji, Miho Otani, Masako Nakano, Yusaku Saijo, Hikaru Tsuchida, Shinichi Ishioka, Akira Nishikawa, Tsuyoshi Saito, Mineko Fujimiya
    SCIENTIFIC REPORTS 7 (1) 2045-2322 2017/08 [Refereed][Not invited]
     
    Bone marrow-derived mesenchymal stem cells (BM-MSC) has been applied as the most valuable source of autologous cell transplantation for various diseases including diabetic complications. However, hyperglycemia may cause abnormalities in intrinsic BM-MSC which might lose sufficient therapeutic effects in diabetic patients. We demonstrated the functional abnormalities in BM-MSC derived from both type 1 and type 2 diabetes models in vitro, which resulted in loss of therapeutic effects in vivo in diabetic nephropathy (DN). Then, we developed a novel method to improve abnormalities in BM-MSC using human umbilical cord extracts, namely Wharton's jelly extract supernatant (WJs). WJs is a cocktail of growth factors, extracellular matrixes and exosomes, which ameliorates proliferative capacity, motility, mitochondrial degeneration, endoplasmic reticular functions and exosome secretions in both type 1 and type 2 diabetes-derived BM-MSC (DM-MSC). Exosomes contained in WJs were a key factor for this activation, which exerted similar effects to complete WJs. DM-MSC activated by WJs ameliorated renal injury in both type 1 and type 2 DN. In this study, we developed a novel activating method using WJs to significantly increase the therapeutic effect of BM-MSC, which may allow effective autologous cell transplantation.
  • 齋藤 悠城, 千見寺 貴子, 松村 崇史, 水江 由佳, 藤宮 峯子
    理学療法学Supplement 公益社団法人 日本理学療法士協会 2016 455 - 455 2017 

    【はじめに，目的】

    多発性筋炎に対する運動は炎症の助長や異所性組織を形成させるリスクがある。本研究では，骨格筋の炎症と再生，異所性組織形成において中心的な役割を果たす骨格筋間葉系前駆細胞（Mesenchymal Progenitor Cell：MPC）を中心として，運動刺激と炎症の助長および異所性組織形成のメカニズムを解明する。

    【方法】

    8週齢のBalb/cマウス（メス）に精製したBalb/c由来ミオシンと完全フロイトアジュバントをリンパ節付近に免疫し実験的自己免疫性筋炎（Experimental Autoimmune Myositis：EAM）を作成した。免疫後5週の時点で運動介入（17m/min，-20度傾斜，30分）を実施し，24時間後に腓腹筋を採取した。MPCはLineage陰性α7-integrin陰性PDGFRα陽性の細胞を単離した。単離後のMPCにて遺伝子発現解析（筋再生関連遺伝子Follistatin，IGF-1；抗炎症関連遺伝子TSG-6，IL-1Ra，IL-33；細胞周期阻害遺伝子p16ink4a；線維化関連遺伝子αSMA，TGFβ）を実施した。Control（Con）群，Controlに運動介入をしたControl-Exercise（Con-Ex）群，EAM群，EAMに運動介入をしたEAM-Exercise（EAM-Ex）群の4群で比較検討した。

    【結果】

    片側腓腹筋あたりのMPC細胞数はCon群（mean，0.37*105 cells；95% CI，0.24-0.50）と比較してEAM群（mean，2.02 *105 cells；95% CI，1.58-2.46）で有意に増加した（P＜0.001）。Con群ではEx介入によってMPC細胞数は有意に増加したが（mean，0.70 *105 cells；95% CI，0.56-0.84；P=0.02），EAM群ではEx介入で細胞数に有意差はなかった（mean，1.52 *105 cells；95% CI，1.90-1.14；P=0.132）。MPCの遺伝子発現解析の結果，Con-Ex群では筋再生関連，抗炎症関連および細胞周期阻害遺伝子の発現量増加を認めたが（P＜0.05），EAM-Ex群減少（P＜0.05），もしくは変化しなかった。さらに，EAM-Ex群では線維化関連遺伝子の発現が増加した（P＜0.05）。

    【結論】

    正常マウスと筋炎マウスのMPCでは運動刺激に対する反応が異なることを明らかにした。慢性炎症状態の骨格筋に対する運動刺激は，本来MPCがもつ抗炎症能力や筋サテライト細胞ニッチとしての働きを減弱させること，MPCにおけるαSMAやTGFβの発現が亢進させて骨格筋線維化の原因となる可能性を示唆した。また，間葉系細胞におけるp16ink4aの発現低下は様々な組織における線維化や再生不良に寄与することが報告されている。今回，筋炎モデルでは運動介入によってp16ink4a発現低下を認め，正常モデルでは発現亢進した。以上より，p16ink4aは適切な運動刺激のバイオマーカーとなるかもしれない。

  • Kanna Nagaishi, Yuka Mizue, Takako Chikenji, Miho Otani, Masako Nakano, Naoto Konari, Mineko Fujimiya
    SCIENTIFIC REPORTS 6 2045-2322 2016/10 [Refereed][Not invited]
     
    Bone marrow-derived mesenchymal stem cells (MSCs) have contributed to the improvement of diabetic nephropathy (DN); however, the actual mediator of this effect and its role has not been characterized thoroughly. We investigated the effects of MSC therapy on DN, focusing on the paracrine effect of renal trophic factors, including exosomes secreted by MSCs. MSCs and MSC-conditioned medium (MSC-CM) as renal trophic factors were administered in parallel to high-fat diet (HFD)-induced type 2 diabetic mice and streptozotocin (STZ)-induced insulin-deficient diabetic mice. Both therapies showed approximately equivalent curative effects, as each inhibited the exacerbation of albuminuria. They also suppressed the excessive infiltration of BMDCs into the kidney by regulating the expression of the adhesion molecule ICAM-1. Proinflammatory cytokine expression (e.g., TNF-alpha) and fibrosis in tubular interstitium were inhibited. TGF-beta 1 expression was down-regulated and tight junction protein expression (e.g., ZO-1) was maintained, which sequentially suppressed the epithelial-to-mesenchymal transition of tubular epithelial cells (TECs). Exosomes purified from MSC-CM exerted an anti-apoptotic effect and protected tight junction structure in TECs. The increase of glomerular mesangium substrate was inhibited in HFD-diabetic mice. MSC therapy is a promising tool to prevent DN via the paracrine effect of renal trophic factors including exosomes due to its multifactorial action.
  • Masako Nakano, Kanna Nagaishi, Naoto Konari, Yuki Saito, Takako Chikenji, Yuka Mizue, Mineko Fujimiya
    Scientific Reports 6 (1) 2045-2322 2016/04 [Refereed][Not invited]
  • Takako Chikenji, Anne Gingery, Chunfeng Zhao, Matthias Vanhees, Tamami Moriya, Ramona Reisdorf, Kai-Nan An, Peter C. Amadio
    PLOS ONE 9 9 (9) e108312  1932-6203 2014/09 [Refereed][Not invited]
     
    Carpal tunnel syndrome (CTS) is an idiopathic disease that results from increased fibrosis of the subsynovial connective tissue (SSCT). A recent study found overexpression of both transforming growth factor-beta (TGF-beta) and connective tissue growth factor (CTGF) in the SSCT of CTS patients. This study investigated TGF-beta and CTGF expression in a rabbit model of CTS, in which SSCT fibrosis is induced by a surgical injury. Levels of TGF-beta 1 and CTGF at 6, 12, 24 weeks after injury were determined by immunohistochemistry A significant increase in TGF-beta 1 and a concomitant significant increase in CTGF were found at 6 weeks, in addition to higher cell density compared to normal (all p<0.05), Interestingly, CTGF expression was reduced at 12 and 24 weeks, suggesting that an initial insult results in a time limited response. We conclude that this rabbit model mimics the fibrosis found in human CTS, and may be useful to study pathogenetic mechanisms of CTS in vivo.
  • Takako Chikenji, Anne Gingery, Chunfeng Zhao, Sandra M. Passe, Yasuhiro Ozasa, Dirk Larson, Kai-Nan An, Peter C. Amadio
    JOURNAL OF ORTHOPAEDIC RESEARCH 1 32 (1) 116 - 122 0736-0266 2014/01 [Refereed][Not invited]
     
    Non-inflammatory fibrosis of the subsynovial connective tissue (SSCT) is a hallmark of carpal tunnel syndrome (CTS). The etiology of this finding and its relationship to the development of CTS remain poorly understood. Recent studies have found that transforming growth factor-beta (TGF-beta) plays a central role in fibrosis. The purpose of this study was to investigate the expression of TGF-beta and connective tissue growth factor (CTGF), a downstream mediator of TGF-beta, in the pathogenesis of CTS. We compared SSCT specimens from 26 idiopathic CTS patients with specimens from 10 human cadaver controls with no previous diagnosis of CTS. Immunohistochemistry was performed to determine levels TGF-beta 1, CTGF, collagen 1(Col1) and collagen 3 (Col3) expression. TGF-beta 1 (p<0.01), CTGF (p<0.01), and Col3 (p<0.01) were increased in SSCT of CTS patients compared with control tissue. In addition, a strong positive correlation was found between TGF-beta 1 and CTGF, (R-2 - 0.80, p<0.01) and a moderate positive correlation between Col3 and TGF-beta 1 (R-2 = 0.49, p<0.01). These finding suggest that there is an increased expression of TGF-beta and CTGF, a TGF-beta regulated protein, and that this TGF-beta activation may be responsible for SSCT fibrosis in CTS patients. (C) 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
  • Kousuke Iba, Yasuhisa Abe, Takako Chikenji, Kumiko Kanaya, Hironori Chiba, Koichi Sasaki, Takayuki Dohke, Takuro Wada, Toshihiko Yamashita
    JOURNAL OF BONE AND MINERAL METABOLISM 31 (4) 399 - 408 0914-8779 2013/07 [Refereed][Not invited]
     
    Tetranectin is a plasminogen-binding protein that enhances plasminogen activation, which has been suggested to play a role in tissue remodeling. Recently, we showed that tetranectin has a role in the wound-healing process. In this study, we investigated whether tetranectin plays a role in fracture healing. The fracture-healing process was studied using a femoral osteotomy model in tetranectin-null mice, previously generated by the authors. Radiographic imaging, micro-computed tomography (mu CT), and histological analysis were used to evaluate osteotomy healing. In wild-type mice, a callus was apparent from 7 days, and most samples showed marked callus formation and rebridging of the cortices at the osteotomy site at 21 days. In contrast, in the tetranectin-null mice there was no callus formation at 7 days and much less callus formation and no bridging of cortices were observed at 21 days. At 35 days, all osteotomy sites showed clear rebridging, and secondary bone formation was achieved in wild-type mice by 42 days. In contrast, no clear rebridging or secondary bone formation was observed at 42 days in the tetranectin-null mice. Analysis using mu CT at 21 days after osteotomy revealed that the callus area in tetranectin-null mice was smaller than that in wild-type mice. Histological analysis also showed that soft tissue and callus formation were smaller in the tetranectin-null mice at the early stage of the healing process after drill-hole injury. These results suggested that tetranectin could have a role in the positive regulation at the early stage of the fracture-healing process, which was reflected in the delayed fracture healing in tetranectin-deficient mice.
  • Vanhees M, Chikenji T, Thoreson AR, Zhao C, Schmelzer JD, Low PA, An KN, Amadio PC
    Hand (New York, N.Y.) 1 8 (1) 54 - 59 1558-9447 2013/03 [Refereed][Not invited]
  • Hayashi M, Zhao C, Thoreson AR, Chikenji T, Jay GD, An KN, Amadio PC
    PloS one 12 8 (12) e83836  2013 [Refereed][Not invited]
  • Adam A. Sassoon, Yasuhiro Ozasa, Takako Chikenji, Yu-Long Sun, Dirk R. Larson, Mary L. Maas, Chunfeng Zhao, Jin Jen, Peter C. Amadio
    JOURNAL OF ORTHOPAEDIC RESEARCH 30 (11) 1710 - 1718 0736-0266 2012/11 [Refereed][Not invited]
     
    This study investigated the comparative ability of bone marrow and skeletal muscle derived stromal cells (BMSCs and SMSCs) to express a tenocyte phenotype, and whether this expression could be augmented by growth and differentiation factor-5 (GDF-5). Tissue harvest was performed on the hind limbs of seven dogs. Stromal cells were isolated via serial expansion in culture. After four passages, tenogenesis was induced using either ascorbic acid alone or in conjunction with GDF-5. CD44, tenomodulin, collagen I, and collagen III expression levels were compared for each culture condition at 7 and 14 days following induction. Immunohistochemistry (IHC) was performed to evaluate cell morphology and production of tenomodulin and collagen I. SMSCs and BMSCs were successfully isolated in culture. Following tenocytic induction, SMSCs demonstrated an increased mean relative expression of tenomodulin, collagen I, and collagen III at 14 days. BMSCs only showed increased mean relative expression of collagen I, and collagen III at 14 days. IHC revealed positive staining for tenomodulin and collagen I at 14 days for both cell types. The morphology of skeletal muscle derived stromal cells at 14 days had an organized appearance in contrast to the haphazard arrangement of the bone marrow derived cells. GDF-5 did not affect gene expression, cell staining, or cell morphology significantly. Stromal cells from either bone marrow or skeletal muscle can be induced to increase expression of matrix genes; however, based on expression of tenomodulin and cell culture morphology SMSCs may be a more ideal candidate for tenocytic differentiation. (c) 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:17101718, 2012
  • Yu-Long Sun, Tamami Moriya, Chunfeng Zhao, Ramona L. Kirk, Takako Chikenji, Sandra M. Passe, Kai-Nan An, Peter C. Amadio
    JOURNAL OF ORTHOPAEDIC RESEARCH 30 (4) 649 - 654 0736-0266 2012/04 [Refereed][Not invited]
     
    The most common histological finding in carpal tunnel syndrome (CTS) is non-inflammatory fibrosis and thickening of the subsynovial connective tissue (SSCT) in the tunnel. While the cause of SSCT fibrosis and the relationship of SSCT fibrosis and CTS are unknown, one hypothesis is that SSCT injury causes fibrosis, and that the fibrosis then leads to CTS. We investigated the sensitivity of the SSCT to injuries. Two types of surgical interventions were performed in a rabbit model: A skin incision with tendon laceration and SSCT stretching sufficient to damage the SSCT, and skin incision alone. Twelve weeks after surgery, the rabbit carpal tunnel tissues were studied with immunochemistry for TGF-beta receptors 1, 2, and 3, collagen III, and collagen VI. All TGF-beta receptors were expressed. The percentages of the TGF-beta receptors' expressions were less in the control SSCT fibroblasts than in the fibroblasts from rabbits with surgical interventions. The surgical interventions did not result in any alteration of collagen III expression. However, both surgical interventions resulted in a significant decrease in collagen VI expression compared to the control group. The two surgical interventions achieved similar expression of TGF-beta receptors and collagens. Our results provide evidence that the SSCT is sensitive to surgical interventions, even when these are modest. Since SSCT fibrosis is a hallmark of CTS, these data also suggest that such fibrosis could result from relatively minor trauma. (C) 2011 Orthopaedic Research Society. (C) 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:649654, 2012
  • Atsushi Teramoto, Daisuke Suzuki, Tomoaki Kamiya, Takako Chikenji, Kota Watanabe, Toshihiko Yamashita
    AMERICAN JOURNAL OF SPORTS MEDICINE 39 (10) 2226 - 2232 0363-5465 2011/10 [Refereed][Not invited]
     
    Background: Suture-button fixation for tibiofibular syndesmosis injuries is a relatively new surgical technique thought to provide semirigid dynamic stabilization. However, adequate information is still not available and there are controversies as to whether it provides enough fixation for syndesmosis injuries. Hypothesis: Optimally directed suture-button fixation brings physiologic dynamic stabilization of the ankle syndesmosis. Study Design: Controlled laboratory study. Methods: Stabilization of the ankle syndesmosis fixed by a suture-button construct was examined using 6 normal fresh-frozen cadaver legs. After initial tests of intact and injured models, suture-button fixation and screw surgical techniques were performed sequentially for each specimen, with single suture-button fixation, double suture-button fixation, anatomic suture-button fixation, and metal screw. Anterior and medial traction forces, as well as external rotation force, were applied to the tibia; the diastasis of the syndesmosis and the rotational angle of the fibula related to the tibia were measured using a magnetic tracking system. Results: Each traction and rotation force significantly increased the diastasis and fibular rotational angles in the created injury models. With single fixation, the diastases increased significantly compared with the intact model with an anterior traction force (P < .001), a medial traction force (P = .005), and an external rotation force (P = .015). The fibular rotational angles increased significantly with a medial traction force (P = .005) and an external rotation force (P < .001). With double fixation, the diastases increased significantly with a medial traction force (P = .004) and an external rotation force (P = .012). The fibular rotational angles increased significantly with a medial traction force (P = .035) and an external rotation force (P = .002). With anatomic fixation, there were no significant differences compared with the intact model. With the metal screw, the diastases decreased significantly with an external rotation force (P = .037). Conclusion: Neither single nor double fixation for syndesmosis injuries provided multidirectionally stabilizing syndesmosis. Anatomic fixation directed from the posterior cortex of the fibula to the anterolateral edge of the tibia allowed dynamic stabilization of intact cadaver specimens. The metal screw provided very rigid fixation. Clinical Relevance: Optimal direction of the suture button can provide adequate stabilization of the ankle and could benefit athletes with syndesmosis injuries.
  • Naoki Oikawa, Sadako Tsubota, Takako Chikenji, Gyoku Chin, Mitsuhiro Aoki
    HONG KONG JOURNAL OF OCCUPATIONAL THERAPY 21 (1) 41 - 46 1569-1861 2011/06 [Refereed][Not invited]
     
    Objective: Musicians tend to suffer from playing-related musculoskeletal problems over the forearm muscles. Lateral epicondylitis of the elbow is the most common disease of pianists. The purpose of this study was to measure by electromyography (EMG) the wrist extensor and flexor to clarify the burden of forearm muscles during piano playing with various wrist positions.Methods: Fourteen female piano students and 14 novice females participated in this study. Surface EMG was conducted during piano playing in nine conditions that combined three wrist positions with three degrees of loudness.Results: The muscle activities increased with the increase of loudness in both groups. Muscle activities for both the wrist extensor and flexor were the smallest in the neutral wrist position. There were no differences of the muscle activities between the piano-student and the control groups.Conclusion: The neutral position of the wrist should be recommended for reduction of playing-related musculoskeletal burden during piano playing. Copyright (C) 2011, Elsevier (Singapore) Pte. Ltd. All rights reserved.
  • Tamami Moriya, Takako Chikenji, Andrew R. Thoreson, Chunfeng Zhao, Kai-Nan An, Peter C. Amadio
    JOURNAL OF BIOMECHANICS 44 (7) 1414 - 1416 0021-9290 2011/04 [Refereed][Not invited]
     
    The purpose of this study was to investigate the effects of temperature, velocity and load on the gliding resistance (GR) of flexor digitorum profundus (FOP) tendons in a human cadaver model. A total of 40 FDP tendons from the index through small digits of ten human cadavers were tested to assess the effect of temperature (4,23 or 36 degrees C), velocity (2, 4, 6, 8, 10 or 12 mm/s) and load (250, 500, 750, 1000, 1250 and 1500 g) on GR. The mean GR at 4 degrees C was significantly higher than the mean GR at 36 degrees C (p<0.0066). There was no significant difference in the mean GR of the tested velocities. The mean GR was proportional to load, with each successive load having significantly higher GR than the loads before it (all p<0.001). There was no significant difference in the mean GR by digit. In this in vitro model, we have demonstrated that tendon gliding resistance is proportional to load, independent of velocity and somewhat affected by temperature. We conclude that it is important to specify these conditions when reporting gliding resistance, especially load and temperature. (C) 2011 Elsevier Ltd. All rights reserved.
  • Takako Chikenji, Richard A. Berger, Mineko Fujimiya, Daisuke Suzuki, Sadako Tsubota, Kai-Nan An
    JOURNAL OF HAND SURGERY-AMERICAN VOLUME 36A (3) 406 - 412 0363-5023 2011/03 [Refereed][Not invited]
     
    Purpose To examine the distribution of encapsulated nerve endings called mechanoreceptors in the human distal interphalangeal (DIP) joint and surrounding structures. Methods We processed 12 right index finger DIP joints and surrounding structures from fresh cadavers for immunohistochemistry of the anti-protein gene product 9.5 (PGP9.5) and silver staining to detect encapsulated nerve endings. Serial transverse sections were cut throughout the whole specimen and divided into 3 regions along the longitudinal axis: distal, middle, and proximal. Each of the transverse sections was partitioned into dorsal capsule (DC), radial capsule (RC), ulnar capsule (UC), volar plate (VP), and radial and ulnar assemblage nuclei (RAN and UAN); the RAN and UAN are located on both the radial and ulnar side of the VP. The C3 pulley contained the proximal region of the RAN and UAN, whereas the AS pulley contained the middle and distal. The accessory collateral ligament contained all the regions of the RAN and UAN. We analyzed and compared the density of encapsulated nerve endings among the 18 different regions. Results According to the modified Freeman and Wyke classification, we identified type I (eg, Ruffini-like endings) and type II (eg, Pacini-like endings) nerve endings. The density of type II nerve endings in the proximal region of the RAN and UAN was considerably higher than that in the proximal region of the VP, RC, UC and DC, and that in the proximal region of the VP, RC, UC, and DC, respectively. Conclusions Our examination of the distribution of type I and type II nerve endings provides new information on the sensory systems of the DIP joints and surrounding structures. (J Hand Surg 2011;36A:406-412. Copyright (C) 2011 by the American Society for Surgery of the Hand. All rights reserved.)
  • Takako Chikenji, Daisuke Suzuki, Mineko Fujimiya, Tamami Moriya, Sadako Tsubota
    JOURNAL OF HAND SURGERY-AMERICAN VOLUME 35A (8) 1286 - 1293 0363-5023 2010/08 [Refereed][Not invited]
     
    Purpose To examine the distribution of encapsulated nerve endings called mechanoreceptors in the human proximal interphalangeal (PIP) joint and surrounding structures. Methods We processed 12 right index finger PIP joints and surrounding structures from fresh and dissecting-room cadavers for immunohistochemistry of the anti-protein gene product 9.5 and silver staining to detect encapsulated nerve endings. Serial transverse sections were cut throughout the whole specimen and divided into 3 regions along the longitudinal axis: distal, middle, and proximal. Each of the transverse sections was partitioned into dorsal capsule (DC), radial capsule (RC), ulnar capsule (UC), volar plate (VP), radial assemblage nuclei (RAN), and ulnar assemblage nuclei (UAN); the RAN and UAN are located on the radial and ulnar side of the VP. The Cl pulley contained the proximal region of the RAN and UAN, whereas the A3 pulley contained the middle and distal regions. The accessory collateral ligament contained all the regions of the RAN and UAN. The densities of encapsulated nerve endings in these 18 different regions were analyzed and compared. Results According to the modified Freeman and Wyke classification, type I (Ruffini-like endings) and type II (Pacini-like endings) nerve endings were identified. The density of type I nerve endings in the proximal region of the VP was substantially higher than that in the proximal region of the RAN, UAN, RC, UC, and DC. The density of type II nerve endings in the proximal region of the RAN and UAN was substantially higher than that in the proximal region of the VP, RC, UC and DC, and that in the proximal region of the VP and DC, respectively. Conclusions Our examination of the distribution of type I and type II nerve endings provides information on the sensory systems of the PIP joints and surrounding structures. (J Hand Surg 2010;35A:1286-1293. (C) 2010 Published by Elsevier Inc. on behalf of the American Society for Surgery of the Hand.)
  • Takako Chikenji, Hajime Toda, Chin Gyoku, Naoki Oikawa, Masaki Katayose, Sadako Tsubota
    Journal of Musculoskeletal Research 13 (2) 75 - 82 0218-9577 2010/06 [Refereed][Not invited]
     
    The purpose of this study was to investigate the strengths of four intrinsic muscles of the hand of college baseball players. The strengths of four intrinsic muscle groups were measured by the Rotterdam Intrinsic Hand Myometer (RIHM) which has been developed to assess the abduction of the little finger and index finger, and palmar abduction and opposition of the thumb. The strengths of these four intrinsic muscle groups were compared between baseball players and inexperienced sports players. The abduction of the little finger and index finger, and the opposition of the thumb in both the dominant and nondominant hands of the baseball players were notably stronger than those of the inexperienced sports players. There was no statistical difference in the strength of the palmar abduction of the thumb between the two groups. The results suggest that the specific intrinsic muscles in both the dominant and nondominant hands might be strengthened by repeated baseball practices, such as a batting performance, which requires strain in both the dominant and nondominant hands repeatedly. © 2010 World Scientific Publishing Company.

Conference Activities & Talks

  • Fisetin improved depressive behavior in murine lupus by decreasing senescent neural cell.  [Not invited]
    Yuki Saito, Maki Miyajima, Sena Yamamoto, Tsukasa Sato, Norihiro Miura, Mineko Fujimiya, Takako S. Chikenji
    The 6th International Cell Senescence Association Conference  2021/12
  • Diabetic wound impaired injury-induced transient senescence in adipose tissue that promotes the normal wound healing process.  [Not invited]
    Arisa Kita, Yuki Saito, Norihiro Miura, Sena Yamamoto, Tsukasa Sato, Takatoshi Yotsuyanagi, Mineko Fujimiya, Takako S. Chikenji
    The 6th International Cell Senescence Association Conference  2021/12
  • Dual role of TGF-β1 in cellular senescence of tubular cells and hyper-proliferation of fibroblasts to aggravate lupus nephritis.  [Not invited]
    Shogo Ijima, Yuki Saito, Maki Miyajima, Mineko Fujimiya, Takako S. Chikenji
    The 6th International Cell Senescence Association Conference  2021/12
  • Altered regulation of senescence-associated secretory phenotype in hippocampus modulates resilience to chronic mild psychiatric stress.  [Not invited]
    Tsukasa Sato, Yuki Saito, Maki Miyajima, Sena Yamamoto, Norihiro Miura, Mineko Fujimiya, Takako S. Chikenji
    The 6th International Cell Senescence Association Conference  2021/12
  • Lower-expression of Cdkn2a is associated with dysregulation of fibro-adipogenic progenitors results in skeletal muscle atrophy and degeneration in diabetic mice.  [Not invited]
    Norihiro Miura, Yuki Saito, Arisa Kita, Maki Miyajima, Tsukasa Sato, Sena Yamamoto, Mineko Fujimiya, Takako S. Chikenji
    The 6th International Cell Senescence Association Conference  2021/12
  • Senolysis by fisetin ameliorates lupus erythematosus-like skin lesions in MRL/lpr mice.  [Not invited]
    Sena Yamamoto, Yuki Saito, Shogo Ijima, Arisa Kita, Norihiro Miura, Tsukasa Sato, Maki Miyajima, Mineko Fujimiya, Takako S. Chikenji
    The 6th International Cell Senescence Association Conference  2021/12
  • Accumulation of senescent neurons in mice model of autism spectrum disorder with abnormal synaptic pruning.  [Not invited]
    Maki Miyajima, Yuki Saito, Tsukasa Sato, Norihiro Miura, Sena Yamamoto, Mineko Fujimiya, Takako S. Chikenji
    The 6th International Cell Senescence Association Conference  2021/12
  • 骨格筋再生プロセスにおける細胞老化  [Invited]
    千見寺貴子, 齋藤悠城, 藤宮峯子
    第20回日本再生医療学会 シンポジウム11
  • Exercise enhances skeletal muscle regeneration by promoting senescence in fibro-adipogenic progenitors  [Not invited]
    Yuki Saito, Takashi Matsumura, Mineko Fujimiya, Takako S. Chikenji
    米国整形外科基礎学会 Annual meeting of Orthopaedic Research Society  2020/02
  • Senescent Cells Escape From Immune Clearance In Fibrotic Subsynovial Connective Tissue In Carpal Tunnel Syndrome  [Not invited]
    Takako S. Chikenji, Yuki Saito, Alyssa Vrieze, Sandra Passe, Tamara Tchkonia, James L. Kirkland, Peter C. Amadio, Anne Gingery
    米国整形外科基礎学会 Annual meeting of Orthopaedic Research Society  2020/02
  • 細胞老化と健康寿命  [Invited]
    千見寺貴子
    北海道大学保健科学研究院 公開講座  2019/11
  • 細胞老化ークリアランスーリモデリング連鎖 に基づく新たな再生医療の可能性  [Invited]
    千見寺貴子
    北海道移植免疫研究会  2019/09
  • Role of platelet-derived growth factor (PDGF) signaling in synovial fibrosis.  [Invited]
    Takako S. Chikenji
    FHS International conference  2019/07
  • Platelet-Derived Growth Factor Receptor Alpha Signaling in Carpal Tunnel Syndrome  [Invited]
    Takako S. Chikenji
    Musculoskeletal Research Conference, Mayo Clinic, USA  2019/04
  • A Stimulation of Adult Mesenchymal Stromal Cell Promote Chronic Muscular Inflammation Healing in Multiple Myositis.  [Not invited]
    Chikenji T, Saito Y, Mizue Y, Fujimiya M
    Keystone Symposia Conference. Aging and Mechanisms of Aging-Related Disease.  2017/05
  • The Effects of Estradiol on Mesenchymal Stem Cells in Subsynovial Connective Tissue of Carpal Tunnel Syndrome In vitro.  [Not invited]
    Chikenji T, Saito Y, Ozasa Y, Fujimiya M, Yamashita T, Iba K
    Orthopedic Research Society 63th Annual Meeting.  2017/03
  • 細胞療法に適した間葉系幹細胞の製造法の開発．  [Invited]
    千見寺貴子
    第2回北海道ナノバイオ研究会(HNB)シンポジウム  2016/08
  • Meet the Experience海外学会・研修体験ーグローバル化を目指してー  [Invited]
    千見寺貴子
    日本ハンドセラピィ学会  2016/04
  • Mesenchymal Stem Cells in Subsynovial Connective Tissue in Carpal Tunnel.  [Not invited]
    Chikenji T, Saito Y, Mizue Y, Nagaishi K, Fujimiya M, Yamashita T, Iba K
    Orthopedic Research Society 62th Annual Meeting.  2016/03
  • Transforming growth factor-beta expression is increased in subsynovial connective tissues in human idiopathic carpal tunnel syndrome.  [Not invited]
    Chikenji, T, Gingery, A, Zhao, C, Passe, S, An, K, Amadio, PC
    Orthopedic Research Society 59th Annual Meeting.  2013/02
  • A Comparison of the Strength of the Abduction of the Little and Index fingers and Palmar Abduction and Opposition of the Thumb Between College Baseball Players and Non-athletes.  [Not invited]
    Chikenji, T. Toda, H. Gyoku, C. Oikawa, N. Katayose, M. Ota, H, Tsubota, S
    The 9th Congress of Asian Pacific Federation of Societies for Surgery of the Hand and The 5th Congress of Asian Pacific Federation of Societies for Hand Therapist.  2012/10
  • Absence of Myofibroblasts in Subsynovial Connective Tissues in Human and Rabbit Models of Carpal Tunnel Syndrome.  [Not invited]
    Chikenji, T. Vanhees, M. Moriya, T. Zhao, C. An, K-N, Amadio PC
    the 8th International Hand and Wrist Biomechanics Symposium.  2012/04
  • Fibroblast-Specific Protein 1 is Increased in Subsynovial Connective Tissues in Idiopathic Carpal Tunnel Syndrome.  [Not invited]
    Chikenji, T. Zhao, C. Ettema, A. Sun, Y-L. Hayashi, M, An, K-N, Amadio PC
    Orthopedic Research Society 58th Annual Meeting.  2012/02
  • Absence of Myofibroblasts in Subsynovial Connective Tissues in Human and Rabbit Models of Carpal Tunnel Syndrome.  [Not invited]
    Chikenji, T. Vanhees, M. Moriya, T. Zhao, C. An, K-N, Amadio PC
    Orthopedic Research Society 58th Annual Meeting. San Francisco  2012/02
  • Distribution of Nerve Endings in Distal Interphalangeal Joint and Surrounding Structures.  [Not invited]
    Chikenji T, Berger RA, Fujimiya M, Suzuki D, An K-N, Tsubota S
    Orthopedic Research Society 57th Annual Meeting.  2011/01
  • Distribution of Nerve Endings in Proximal Interphalangeal Joint and Surrounding Structures.
    Chikenji T, Suzuki D, Fujimiya M, Moriya T, Kamiya T, Tsubota S
    Orthopedic Research Society 56th Annual Meeting  2009/03
  • Distribution of nerve fiber and mechanoreceptor in the metacarpophlangeal and proximal interphalangeal joints: a preliminary study.  [Not invited]
    Chikenji T, Suzuki D, Aoki M, Tsubota S, Tatsumi H
    7th Congress of Asian Pacific Federation of Societies for Surgery of the Hand.  2008/02
  • A new rehabilitaion protocol for massive rotator cuff repair.  [Not invited]
    Chikenji, T. Kasai, T. Kubo, S. Mitani, M. Ogawa
    7th congress of the international federation of societies for hand therapy.  2007/03

MISC

  • 【健康寿命の鍵を握る骨格筋 代謝・内分泌を介した全身性制御の分子基盤から運動による抗老化まで】(第3章)個体の老化を支配する骨格筋の老化 運動による細胞老化制御と骨格筋再生
    齋藤 悠城, 千見寺 貴子  実験医学  40-  (2)  222  -228  2022/02  
    細胞老化はがん抑制や個体発生、さらには組織再生など生体に重要な機能を果たす一方で、加齢や慢性炎症などを進展する二面性が報告されている。近年、骨格筋への運動ストレスが細胞老化を誘導して骨格筋の修復や再生を促進するだけでなく、慢性炎症や線維化など骨格筋病態へも関与することが明らかになってきた。細胞老化制御機構の解明は新たな骨格筋治療の突破口になる可能性を秘めている。本稿では、運動による骨格筋の細胞老化と組織修復プロセス、また加齢性疾患における細胞老化の影響、細胞老化を標的とした新規治療法の可能性などについて概説する。(著者抄録)
  • 多発性筋炎に対する高強度伸張性収縮トレーニングの安全性と有効性の検討
    檜森 弘一, 芦田 雪, 舘林 大介, 齋藤 悠城, 千見寺 貴子, 山田 崇史  理学療法学  47-  (Suppl.1)  124  -124  2021/03
  • 多発性筋炎に対する高強度伸張性収縮トレーニングの安全性と有効性の検討
    檜森 弘一, 芦田 雪, 舘林 大介, 齋藤 悠城, 千見寺 貴子, 山田 崇史  理学療法学  47-  (Suppl.1)  124  -124  2021/03
  • 間葉系間質/幹細胞が誘導する細胞老化-クリアランス-リモデリング連鎖は慢性腎不全の線維化を改善する
    千見寺 貴子, 齋藤 悠城, 中野 正子, 北 愛里紗, 藤宮 峯子  日本腎臓学会誌  62-  (4)  260  -260  2020/07
  • 射場 浩介, 齋藤 悠城, 千見寺 貴子, 山下 敏彦  Orthopaedics  33-  (4)  7  -12  2020/04  [Not refereed][Not invited]
     
    手根管症候群の主な病態として、手根管内圧の上昇が考えられている。内圧が上昇する原因として、手根管周囲結合組織の肥厚や線維化が報告されている。結合組織線維化の発生機序の一つに滑膜下結合組織(subsynovial connective tissue:SSCT)の線維化が注目されている。SSCT線維化の発症機序として、トランスフォーミング増殖因子ベータ(TGFβ)の発現増強や血小板成長因子受容体(PDGFR)の活性化が関与していることがこれまでの研究で明らかとなってきた。また、これらの下流因子に対する阻害薬は、SSCT由来細胞の増殖および線維化関連遺伝子の発現を調節する効果を有していた。このことは、TGFβやPDGFRに対する調節因子がSSCTの線維化を抑制することで、手根管症候群の発症や病態の進行を予防するとともに、症状の改善効果を有する可能性を示唆している。手根管症候群の病態におけるSSCT線維化について、今後のさらなる研究成果が新しい治療法の開発につながることに期待する。(著者抄録)
  • 松村 崇史, 千見寺 貴子, 齋藤 悠城, 小笹 泰宏, 鈴木 智之, 寺本 篤史, 藤宮 峯子, 山下 敏彦  日本整形外科学会雑誌  93-  (8)  S1743  -S1743  2019/09  [Not refereed][Not invited]
  • 千見寺 貴子, 齋藤 悠城, 藤宮 峯子  腎臓内科・泌尿器科  9-  (4)  323  -326  2019/04  [Not refereed][Not invited]
  • 松村 崇史, 齋藤 悠城, 千見寺 貴子, 鈴木 智之, 小笹 泰宏, 寺本 篤史, 藤宮 峯子, 山下 敏彦  日本整形外科学会雑誌  93-  (2)  S304  -S304  2019/03  [Not refereed][Not invited]
  • 松村 崇史, 小笹 泰宏, 寺本 篤史, 山下 敏彦, 齋藤 悠城, 千見寺 貴子, 藤宮 峯子, 鈴木 智之  北海道整形災害外科学会雑誌  61-  (136th suppl)  28  -28  2019
  • 松村 崇史, 小笹 泰宏, 鈴木 智之, 寺本 篤史, 山下 敏彦, 千見寺 貴子, 齋藤 悠城, 藤宮 峯子  北海道整形災害外科学会雑誌  61-  (137th suppl)  13  -13  2019
  • 松村 崇史, 千見寺 貴子, 齋藤 悠城, 小笹 泰宏, 鈴木 智之, 寺本 篤史, 山下 敏彦, 藤宮 峯子  日本整形外科学会雑誌  92-  (8)  S1659  -S1659  2018/08  [Not refereed][Not invited]
  • AMPキナーゼ活性による手根管症候群滑膜下結合組織由来細胞の増殖・線維化関連因子・力学刺激感受性の制御
    齋藤 悠城, 千見寺 貴子, 小笹 泰宏, アン・ギンガリー, 藤宮 峯子, 山下 敏彦, 射場 浩介  日本手外科学会雑誌  35-  (1)  S494  -S494  2018/04
  • 竹原 敦, 小川 敬之, 上城 憲司, 高木 雅之, 田平 隆行, 千見寺 貴子, 西浦 裕子, 西田 征治, 安田 大典, 山口 智晴, 磯 ふみ子, 市川 誠, 井上 忠俊, 木下 遥, 高坂 駿, 越當 美智子, 近藤 真知子, 坂上 真理, 坂本 千晶, 渋谷 晋太郎, 下田 佳央莉, 菅沼 一平, 仙波 梨沙, 田中 純子, 爲近 岳夫, 土谷 里織, 新中 浩司, 藤岡 崇, 古井 香苗, 松尾 崇史, 松尾 涼太, 村井 達彦, 山田 真季, 米澤 武人  作業療法  37-  (1)  3  -11  2018/02  [Not refereed][Not invited]
  • 松村 崇史, 千見寺 貴子, 齋藤 悠城, 小笹 泰宏, 鈴木 智之, 寺本 篤史, 山下 敏彦, 藤宮 峰子  北海道整形災害外科学会雑誌  60-  (135th suppl)  57  -57  2018
  • 刺激豊かな環境での飼育がアルツハイマー病モデルマウスに与える影響の解析
    中野 正子, 小林 英司, 久保田 健太, 大谷 美穂, 千見寺 貴子, 水江 由佳, 永石 歓和, 藤宮 峯子  Dementia Japan  31-  (4)  573  -573  2017/10  [Not refereed][Not invited]
  • 刺激豊かな環境での飼育がアルツハイマー病モデルマウスに与える影響の解析
    中野 正子, 小林 英司, 久保田 健太, 大谷 美穂, 千見寺 貴子, 水江 由佳, 永石 歓和, 藤宮 峯子  Dementia Japan  31-  (4)  573  -573  2017/10  [Not refereed][Not invited]
  • アルツハイマー病理陽性で非認知症であった症例における反応性アストロサイトの解析
    小林 英司, 中野 正子, 久保田 健太, 樋室 伸顕, 溝口 照悟, 千見寺 貴子, 大谷 美穂, 水江 由佳, 永石 歓和, 藤宮 峯子  Dementia Japan  31-  (4)  578  -578  2017/10  [Not refereed][Not invited]
  • 千見寺 貴子, 齋藤 悠城  北海道作業療法  34-  (3)  119  -123  2017/10  [Not refereed][Not invited]
     
    筋骨格系のリハビリテーションにおいて力学的刺激は、古くから機能の回復や症状の緩和をする手段として用いられてきた。近年、基礎研究分野において、力学的刺激そのものが細胞に及ぼす影響を明らかにする「メカノバイオロジー」が発展してきた。これにより、従来リハビリテーションで利用されてきた力学的刺激が、組織のホメオスタシスまたは、損傷治癒を積極的に促進することが示されるようになり、新たに、細胞レベルで組織修復とリモデリングを促す力学的治療介入として「メカノセラピィー」が注目されている。本報告では、基礎研究としてのメカノバイオロジーが、どのようにメカノセラピィーに活かされるのか、文献的考察を加えて報告する。(著者抄録)
  • 糖尿病治療薬メトフォルミンはAMPキナーゼ活性を介して手根管症候群の滑膜下結合組織由来細胞における増殖・線維化関連因子発現を抑制する
    齋藤 悠城, 千見寺 貴子, 小笹 泰宏, Gingery Anne, 藤宮 峯子, 射場 浩介, 山下 敏彦  日本整形外科学会雑誌  91-  (8)  S1790  -S1790  2017/08  [Not refereed][Not invited]
  • PI3KおよびMEK1/2阻害剤が手根管症候群の滑膜下結合組織由来細胞の増殖・線維化関連遺伝子に与える影響
    齋藤 悠城, 千見寺 貴子, 小笹 泰宏, Gingery Anne, 藤宮 峯子, 射場 浩介, 山下 敏彦  日本整形外科学会雑誌  91-  (8)  S1790  -S1790  2017/08  [Not refereed][Not invited]
  • 多発性筋炎モデルマウスにおける骨格筋間葉系前駆細胞の異常性と運動刺激が与える影響
    齋藤 悠城, 千見寺 貴子, 松村 崇史, 水江 由佳, 藤宮 峯子  理学療法学  44-  (Suppl.2)  O  -2  2017/04  [Not refereed][Not invited]
  • 特発性手根管症候群における滑膜下結合組織の間葉系幹細胞に対するエストロゲンの影響 in vitro解析
    千見寺 貴子, 齋藤 悠城, 小笹 泰宏, 山下 敏彦, 射場 浩介  日本手外科学会雑誌  34-  (1)  S572  -S572  2017/04  [Not refereed][Not invited]
  • 手根管症候群における滑膜下結合組織間葉系細胞の増殖抑制 PI3KおよびMEK1/2経路阻害の効果
    齋藤 悠城, 千見寺 貴子, 小笹 泰宏, 山下 敏彦, 射場 浩介  日本手外科学会雑誌  34-  (1)  S636  -S636  2017/04  [Not refereed][Not invited]
  • 齋藤 悠城, 千見寺 貴子, 小笹 泰宏, 藤宮 峯子, 射場 浩介, 山下 敏彦  日本整形外科学会雑誌  91-  (3)  S956  -S956  2017/03  [Not refereed][Not invited]
  • 注意機能低下は上肢外傷の労働災害事故発生の個人的要因となりうるか CATを用いた労災外傷患者と変性疾患患者の注意機能の比較
    白戸 力弥, 加藤 正巳, 千見寺 貴子, 太田 久晶, 石合 純夫  日本作業療法学会抄録集  50回-  OD  -6  2016/09  [Not refereed][Not invited]
  • 花香 恵, 射場 浩介, 千見寺 貴子, 道家 孝幸, 山下 敏彦  日本整形外科学会雑誌  90-  (8)  S1432  -S1432  2016/08  [Not refereed][Not invited]
  • 千見寺 貴子, 齋藤 悠城, 藤宮 峯子, 山下 敏彦, 射場 浩介  日本整形外科学会雑誌  90-  (8)  S1550  -S1550  2016/08  [Not refereed][Not invited]
  • 齋藤 悠城, 千見寺 貴子, 藤宮 峯子, 山下 敏彦, 射場 浩介  日本整形外科学会雑誌  90-  (8)  S1756  -S1756  2016/08  [Not refereed][Not invited]
  • 永石 歓和, 水江 由佳, 千見寺 貴子, 中野 正子, 小成 直人, 藤宮 峯子, 大谷 美穂  北海道外科雑誌  61-  (1)  126  -127  2016/06  [Not refereed][Not invited]
  • 中野 正子, 小成 直人, 齋藤 悠城, 千見寺 貴子, 水江 由佳, 永石 歓和, 藤宮 峯子, 大谷 美穂  北海道外科雑誌  61-  (1)  126  -126  2016/06  [Not refereed][Not invited]
  • 玉 珍, 及川 直樹, 千見寺 貴子, 青木 光広, 坪田 貞子  臨床整形外科  51-  (4)  353  -358  2016/04  [Not refereed][Not invited]
     
    背景:ピアノ演奏家の手内筋筋力は知られていない.本研究では女性のピアノ専攻学生とピアノ非経験者の手内筋筋力を比較し,ピアノ専攻者の手内筋の特徴を調べた.対象と方法:ピアノ専攻者16名(19.8歳)とピアノ非経験者17名(対照:21.5歳)の握力とピンチ力,手内筋筋力をJAMAR Hand DynamometerとRotterdam筋力計で測定した.結果:握力はピアノ専攻群と対照群間に差がなく,両群内の利き手非利き手の差もなかった.ピンチ力と手内筋力はピアノ専攻群が対照群より大きかった(P<0.01).まとめ:ピアノ専攻群の左右の手内筋は発達しており演奏に適していた.(著者抄録)
  • 骨髄間葉系幹細胞はエクソソームを分泌し糖尿病性認知症を改善させる
    中野 正子, 小成 直人, 齋藤 悠城, 千見寺 貴子, 水江 由佳, 永石 歓和, 藤宮 峯子  糖尿病  59-  (Suppl.1)  S  -295  2016/04  [Not refereed][Not invited]
  • 特発性手根管症候群における滑膜下結合組織の間葉系幹細胞の検討
    千見寺 貴子, 齋藤 悠城, 藤宮 峯子, 山下 敏彦, 射場 浩介  日本手外科学会雑誌  33-  (1)  S617  -S617  2016/04  [Not refereed][Not invited]
  • 特発性手根管症候群の滑膜下結合組織におけるPlatelet-derived Growth Factor Receptor alpha陽性細胞の検討
    齋藤 悠城, 千見寺 貴子, 藤宮 峯子, 山下 敏彦, 射場 浩介  日本手外科学会雑誌  33-  (1)  S618  -S618  2016/04  [Not refereed][Not invited]
  • 白戸 力弥, 加藤 正巳, 千見寺 貴子, 太田 久晶, 渡邊 祐大, 石合 純夫, 入船 秀仁, 金谷 耕平, 射場 浩介, 山下 敏彦  日本整形外科学会雑誌  90-  (3)  S656  -S656  2016/03  [Not refereed][Not invited]
  • 小林 萬里, 千見寺 貴子, 石川 竜之介, 齋藤 悠城, 山下 敏彦, 射場 浩介  日本整形外科学会雑誌  90-  (3)  S671  -S671  2016/03  [Not refereed][Not invited]
  • 白戸 力弥, 千見寺 貴子, 渡邊 祐大, 射場 浩介, 金谷 耕平, 山下 敏彦  日本手外科学会雑誌  32-  (3)  282  -285  2015/12  [Not refereed][Not invited]
     
    労働災害事故による上肢外傷の初回受傷患者の注意機能を,標準注意検査法(CAT)を用いて調査した.対象は不注意により生じた労働災害による上肢外傷の初回受傷患者11例11肢であり,男性9例,女性2例であった.検査時の年齢は46歳,受傷から検査までの期間は21ヵ月であった.結果,11例中9例(82%)に1つ以上のサブテストの成績の低下を認めた.成績の低下が多かった主な3つの課題は,記憶更新検査4桁,聴覚性検出課題とPASAT 2秒条件であった.これらの課題は,注意機能のうち選択的注意,注意の分配や言語性作動記憶を必要とする課題である.これらの注意機能の低下は,労働災害事故による上肢外傷の発生の個人的要因となり得る可能性が示唆された.(著者抄録)
  • 白戸 力弥, 加藤 正巳, 太田 久晶, 千見寺 貴子, 石合 純夫  北海道作業療法  32-  (Suppl.)  81  -81  2015/09  [Not refereed][Not invited]
  • 小林 萬里, 千見寺 貴子, 太田 久晶, 射場 浩介  北海道作業療法  32-  (Suppl.)  122  -122  2015/09  [Not refereed][Not invited]
  • 千見寺 貴子, 射場 浩介, 齋藤 憲, 森元 隆文, 小林 萬里, 山下 敏彦  日本整形外科学会雑誌  89-  (8)  S1787  -S1787  2015/09  [Not refereed][Not invited]
  • 上肢運動器疾患における機能障害と心理学的要因の関連 DASHスコアと運動恐怖
    千見寺 貴子, 射場 浩介  日本手外科学会雑誌  32-  (1)  2  -13  2015/04  [Not refereed][Not invited]
  • 石川 竜乃介, 千見寺 貴子, 加藤 正巳, 白戸 力弥, 渡邊 雄大  北海道作業療法  31-  (Suppl.)  75  -75  2014/09  [Not refereed][Not invited]
  • 長南 行浩, 青木 光広, 千見寺 貴子, 齋藤 悠城, 白戸 力弥, 内山 英一, 山下 敏彦  日本整形外科学会雑誌  88-  (8)  S1669  -S1669  2014/08  [Not refereed][Not invited]
  • 千見寺 貴子, 射場 浩介, 石川 竜之介, 加藤 正巳, 白戸 力弥, 渡邊 祐大, 太田 久明, 森元 隆文, 石合 純夫, 齋藤 悠城, 山下 敏彦  日本整形外科学会雑誌  88-  (8)  S1758  -S1758  2014/08  [Not refereed][Not invited]
  • 齋藤 悠城, 内山 英一, 千見寺 貴子, 高田 雄一, 神谷 智昭, 長南 行浩, 山下 敏彦  日本整形外科学会雑誌  88-  (8)  S1760  -S1760  2014/08  [Not refereed][Not invited]
  • 特発性手根管症候群ウサギモデルの滑膜下結合組織におけるTransforming growth factor-beta発現の検討
    千見寺 貴子, Gingery Anne, Zhao Chunfeng, 森谷 珠美, Amadio Peter  日本手外科学会雑誌  31-  (1)  2  -38  2014/04  [Not refereed][Not invited]
  • 寺本 篤史, 鈴木 大輔, 神谷 智昭, 千見寺 貴子, 渡邉 耕太, 山下 敏彦  北海道整形災害外科学会雑誌  55-  (2)  181  -186  2014/03  [Not refereed][Not invited]
     
    正常未固定凍結人体標本6下肢(右3肢、左3肢、平均年齢84.3歳)の踵骨と中足骨を特製木製フレームに、膝関節を伸展位にてそれぞれSteinmann pinにて固定した。正常標本・Syndesmosis損傷モデル・脛腓間固定(Single suture-button固定・Double suture-button固定・Anatomic suture-button固定・金属スクリュー固定)の順で前方引張・内方引張・足関節外旋トルクの各負荷試験を行い、2つの電磁気センサーを用いて脛腓間距離と腓骨外旋角度を計測した。Syndesmosis損傷モデルは脛腓間距離と腓骨外旋角度ともに増加し、Single・Double固定ではともに腓骨の動きを十分に制御することができなかったが、Anatomic suture-button固定は生理的な脛腓間の安定性が多方向において得ることができた。
  • 太田 久晶, 千見寺 貴子, 白戸 力弥, 加藤 正巳, 石合 純夫  北海道作業療法  30-  (Suppl.)  126  -126  2013/09  [Not refereed][Not invited]
  • 白戸 力弥, 加藤 正巳, 千見寺 貴子, 太田 久晶, 石合 純夫  北海道作業療法  30-  (Suppl.)  134  -134  2013/09  [Not refereed][Not invited]
  • 射場 浩介, 道家 孝幸, 千見寺 貴子, 金谷 久美子, 山下 敏彦  日本整形外科学会雑誌  87-  (8)  S1500  -S1500  2013/08  [Not refereed][Not invited]
  • 齋藤 悠城, 内山 英一, 千見寺 貴子, 佐藤 秀一  日本整形外科学会雑誌  87-  (8)  S1518  -S1518  2013/08  [Not refereed][Not invited]
  • 千見寺 貴子, Gingery Anne, Zhao Chunfeng, Passe Sandra, 小笹 泰宏, An Kai-Nan, Amadio Peter  日本整形外科学会雑誌  87-  (8)  S1550  -S1550  2013/08  [Not refereed][Not invited]
  • 千見寺 貴子, 玉 珍, 及川 直樹, 太田 久晶, 坪田 貞子  北海道作業療法  29-  (Suppl.)  71  -71  2012/09  [Not refereed][Not invited]
  • 太田 久晶, 千見寺 貴子, 加藤 正巳, 白戸 力弥, 石合 純夫  北海道作業療法  29-  (Suppl.)  84  -84  2012/09  [Not refereed][Not invited]
  • 射場 浩介, 阿部 恭久, 千葉 弘規, 金谷 久美子, 佐々木 浩一, 千見寺 貴子, 山下 敏彦  日本整形外科学会雑誌  86-  (8)  S1232  -S1232  2012/08  [Not refereed][Not invited]
  • 林 正徳, Zhao Chunfeng, Thoreson Andrew R., 千見寺 貴子, Jay Gregory D., Warman Matthew L., An Kai-Nan, Amadio Peter C.  日本整形外科学会雑誌  86-  (8)  S1341  -S1341  2012/08  [Not refereed][Not invited]
  • 寺本 篤史, 鈴木 大輔, 藤宮 峯子, 神谷 智昭, 渡邉 耕太, 山下 敏彦, 千見寺 貴子  北海道整形災害外科学会雑誌  53-  (2)  362  -362  2012/03  [Not refereed][Not invited]
  • ピアノ演奏時の前腕および手部の筋活動(第一報) ハンドスパンの違いによる検討
    及川 直樹, 坪田 貞子, 千見寺 貴子, 青木 光広, 山崎 肇  日本作業療法学会抄録集  45回-  P24040  -P24040  2011/06  [Not refereed][Not invited]
  • 遠位脛腓靱帯結合損傷に対するSuture-Button Fixationの固定性評価
    寺本 篤史, 鈴木 大輔, 神谷 智昭, 渡邉 耕太, 山下 敏彦, 千見寺 貴子  北海道整形災害外科学会雑誌  52-  (2)  307  -308  2011/03  [Not refereed][Not invited]
  • ピアノ演奏時の手関節肢位と音量の違いによる手関節伸筋および屈筋の筋活動
    及川 直樹, 千見寺 貴子, 玉 珍, 青木 光広, 坪田 貞子  北海道整形災害外科学会雑誌  52-  (1)  105  -106  2010/08  [Not refereed][Not invited]
  • 足関節不安定における前距腓靱帯と遠位脛腓靱帯の役割
    鈴木 大輔, 藤宮 峯子, 寺本 篤史, 神谷 智昭, 渡邉 耕太, 千見寺 貴子, 藤井 岬  北海道整形災害外科学会雑誌  52-  (1)  112  -112  2010/08  [Not refereed][Not invited]
  • 寺本 篤史, 鈴木 大輔, 神谷 智昭, 千見寺 貴子, 渡邉 耕太, 藤宮 峯子, 山下 敏彦  日本整形外科学会雑誌  84-  (8)  S1221  -S1221  2010/08  [Not refereed][Not invited]
  • 遠位脛腓靱帯結合損傷に対するSuture-Button Fixationの効果 挿入方法の違いによる固定性評価
    寺本 篤史, 鈴木 大輔, 神谷 智昭, 千見寺 貴子, 渡邉 耕太, 山下 敏彦  JOSKAS  35-  (4)  237  -237  2010/06  [Not refereed][Not invited]
  • 足のバイオメカニクス 遠位脛腓靱帯結合損傷に対するSuture-Button Fixationの効果 未固定凍結人体標本を用いた固定性評価
    寺本 篤史, 鈴木 大輔, 神谷 智昭, 千見寺 貴子, 渡邉 耕太, 山下 敏彦  日本足の外科学会雑誌  31-  (1)  S182  -S182  2010/05  [Not refereed][Not invited]
  • 千見寺 貴子, 鈴木 大輔, 藤宮 峯子, 神谷 智昭, 千葉 弘規, 坪田 貞子  解剖学雑誌  85-  (Suppl.)  162  -162  2010/03  [Not refereed][Not invited]
  • 寺本 篤史, 鈴木 大輔, 神谷 智昭, 千見寺 貴子, 渡邉 耕太, 山下 敏彦  日本整形外科学会雑誌  84-  (3)  S79  -S79  2010/03  [Not refereed][Not invited]
  • 千見寺 貴子, 鈴木 大輔, 藤宮 峯子, 青木 光広, 山下 敏彦, 神谷 智昭, 坪田 貞子  日本整形外科学会雑誌  83-  (8)  S1304  -S1304  2009/08  [Not refereed][Not invited]
  • ピアノ演奏時の手関節肢位と音量の違いによる前腕伸筋群の筋活動
    及川 直樹, 千見寺 貴子, 伊藤 かつみ, 青木 光広, 坪田 貞子  日本作業療法学会抄録集  43回-  A2  -3  2009/06  [Not refereed][Not invited]
  • 握力・ピンチ力における手内筋の関与 手の使用方法の違いによる手内筋力の差の検討
    玉 珍, 及川 直樹, 千見寺 貴子, 青木 光広, 坪田 貞子  日本作業療法学会抄録集  43回-  B4  -4  2009/06  [Not refereed][Not invited]
  • 正常手指PIP関節の神経終末分布 組織学的研究
    千見寺 貴子, 青木 光広, 坪田 貞子  日本手の外科学会雑誌  26-  (1)  S133  -S133  2009/03  [Not refereed][Not invited]
  • 千見寺 貴子, 鈴木 大輔, 青木 光広, 坪田 貞子, 山下 敏彦  日本整形外科学会雑誌  82-  (8)  S1226  -S1226  2008/08  [Not refereed][Not invited]
  • ピアニストの演奏時における演奏技法の違いによる上肢に与える影響の調査(第1報) 前腕伸筋群の筋活動と手関節角度の検討
    及川 直樹, 千見寺 貴子, 野坂 利也, 青木 光広, 坪田 貞子  日本作業療法学会抄録集  42回-  P472  -P472  2008/06  [Not refereed][Not invited]
  • 千見寺 貴子, 濱谷 靖敏, 吉崎 晃司, 佐藤 亮太, 畑中 渉, 坪田 貞子  北海道作業療法  24-  (Suppl.)  45  -45  2007/09
  • 橈骨遠位端骨折後変形治癒に対する変形矯正手術の小経験と考察
    浜田 佳孝, 日比野 直仁, 八木 啓輔, 安井 夏生, 高井 宏明, 千見寺 貴子  中部日本整形外科災害外科学会雑誌  49-  (秋季学会)  108  -108  2006/09
  • 宮本 雅文, 八木 省次, 三橋 雅, 西岡 孝, 西庄 俊彦, 千見寺 貴子  中部日本整形外科災害外科学会雑誌  49-  (5)  835  -836  2006/09  
    観血的整復固定術を行った第4・5手根中手関節脱臼・脱臼骨折6例6手12関節(男・平均25.5歳)の成績を報告した。受傷機転は転倒5例、壁殴打1例で、Cain分類type IAが8関節、IBが2関節、IIIが2関節であった。骨折の合併は中手骨基部骨折が4手6関節、有鉤骨前額面骨折が1手2関節であった。手術時期は受傷後平均14.5日で、1例は受傷後53日の陳旧例であった。術後4週以降受診がなかった1例を除き、他の5例は14〜37週経過し、全例疼痛なく、手・手指関節の可動性制限もなく、X線所見で転位は認めず経過良好である。
  • 西庄 俊彦, 宮本 雅文, 八木 省次, 三橋 雅, 千見寺 貴子  中部日本整形外科災害外科学会雑誌  49-  (4)  799  -800  2006/07  
    17歳男。野球の試合で打球を捕球する際、野手と交錯し左肘を打撲し、左肘関節痛が出現した。上腕三頭筋腱断裂と診断された。単純X線写真側面像にて肘頭の近位に小骨片を認めた。MRI T2強調画像で上腕三頭筋腱の肘頭付着部の近位にhigh intensity areaを認めた。受傷後4日後に手術を施行した。手術後3週目から可動域訓練を開始し、術後12週現在、左肘ROM-15°〜120°である。早期に手術療法を行うことで比較的良好な成績を得ることができた。
  • 千見寺 貴子, 夫 一龍, 宮武 重子, 高岡 妙子, 勝田 万紀子  日本褥瘡学会誌  7-  (3)  632  -632  2005/08
  • 宮武 重子, 高岡 妙子, 藤原 愛子, 千見寺 貴子  日本褥瘡学会誌  7-  (3)  687  -687  2005/08

Industrial Property Rights

  • 特開WO2019/208688 A1:生体移植用細胞シート及びその製造方法  
    千見寺貴子
  • 特開WO2018/199194 A1:間葉系幹細胞の賦活化剤への適応性を判定する方法  
    千見寺貴子
  • 特許第6555691号:間葉系幹細胞の賦活化剤、賦活化された間葉系幹細胞およびその製造方法  
    千見寺貴子

Awards & Honors

  • 2020/06 北海道大学大学院保健科学研究院 優秀論文賞
  • 2020/02 Orthopedics Research Society 2020 New Investigator Recognition Award (NIRA), Corresponding author

Research Grants & Projects

  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2023/06 -2026/03 
    Author : 齋藤 貴子, 齋藤 悠城
  • 細胞老化が形成するストレスレジリエンスな細胞社会の解明
    JST：創発的研究支援事業
    Date (from‐to) : 2023 -2026
  • 老化細胞のメカノバイオロジー: メカニカルストレスによる細胞老化制御への挑戦
    日本学術振興会：科学研究費助成事業 挑戦的研究(萌芽)
    Date (from‐to) : 2022/06 -2025/03 
    Author : 齋藤 悠城, 千見寺 貴子
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2021/04 -2024/03 
    Author : 齋藤 悠城 千見寺貴子
     
    老化細胞は安定的に細胞周期を停止する一方、代謝的には非常に活発で、サイトカインやケモカイン、エクソソームなど様々な生物活性を有する因子を分泌する。この分泌現象を老化関連分泌表現型(Senescence-associate secretory phenotype: SASP)と呼ぶ。このSASPによって、老化細胞は周囲の細胞に働きかけて組織を再生へ導く一方で、老化細胞の蓄積は組織の変性や慢性炎症を引き起こすことも知られている。これまでの研究でメカニカルストレスによって細胞老化を制御できる可能性を見い出してきたことから、リハビリテーションによる細胞老化を制御することで組織再生を促す、セノリハビリテーションという新しい治療アプローチの可能性を見出した。本年度は、老化細胞に特徴的なメカニカルストレスの感受性を明らかにするため、in vitroで老化細胞モデルを作成し、その遺伝子発現を解析した。老化細胞において、いくつかの細胞骨格やインテグリンなど、増殖細胞とは異なるいくつかの特徴を明らかにした。また、伸展培養装置を用い、細胞にメカニカルストレスを負荷することで、メカニカルストレスによる細胞老化誘導メカニズムの解明と誘導後の老化細胞の表現系解析を実施した。特定の強度で細胞老化が誘導されること、また慢性炎症状態を模擬した成長因子やサイトカインで刺激と共に、メカニカルストレスを負荷することで、異なる表現系の細胞老化が誘導されることもわかってきた。これらの研究を継続することで、セノリハビリテーションという細胞・分子レベルから細胞老化を制御する新たな治療基盤の創成を目指す。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2021/04 -2024/03 
    Author : 齋藤 (千見寺) 貴子
     
    我々は, 関節リウマチ(Rheumatoid Arthritis; RA)の滑膜で、有害性老化細胞を中心とした微小環境「老化細胞ニッチ」の形成がRA滑膜炎の真の病態像である可能性に辿り着いた。本研究では、1) RA滑膜の老化細胞ニッチを解明する。ついで、2)老化細胞ニッチの破壊による滑膜炎制御法を確立し、炎症を抑えた滑膜に3)組織の再生を促すヘルパー老化細胞を誘導することで滑膜を“関節構造の再生の場”へと変容し、骨・軟骨の再生を促すことで構造的寛解を超えて再び機能する関節の獲得を目指す。 まず、老化細胞ニッチの解明として、RA患者滑膜を採取し、組織学的解析により老化細胞の同定を行った。RA患者滑膜には複数の細胞老化因子を発現する細胞が存在し、周辺に増殖性の高い細胞の集積を認めている。次に、RA患者滑膜の細胞を滑膜組織由来のfibroblast like synoviocytes(FLS)を単離し、老化細胞と非老化細胞の共培養を実施し、老化細胞ニッチの表現形を解析している。In vitroにおいても、RA由来の老化細胞の周辺で増殖性の高い細胞が集積し、アポトーシス抵抗性、MMPなど関節破壊に関連する因子の上昇を認めている。次年度は、老化細胞ニッチ因子ならびにニッチ因子弱点を明らかにするため、老化細胞ニッチの網羅的解析を行うと同時に、候補に上げられたニッチ因子についてノックアウトを行い、細胞ニッチの形成が阻害されるか?老化細胞ニッチを形成する細胞の細胞死を誘導できるか？を検討する。次に、同定した老化細胞ニッチの弱点をもとに、FDA承認薬を用いてニッチ破壊薬の同定を行う。さらに、RAにおいて老化細胞ニッチが関節炎の発症と進行に関与するかを明らかにするため、FLS細胞に細胞老化を誘導し、老化細胞を野生型マウスの膝関節内に投与して、滑膜炎が誘導されるか検討する予定である。
  • 糖尿病性腎症に対する間葉系幹細胞の臨床応用へ向けた品質管理と有効な評価マーカーの確立
    ノーステック 財団：札幌ライフサイエンス産業活性化事業／事業化支援補助金
    Date (from‐to) : 2020/04 -2021/03 
    Author : 千見寺貴子
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
    Date (from‐to) : 2018/04 -2021/03 
    Author : Saito Yuki
     
    Exercise activates muscular stem cells, mesenchymal stem cells (MSCs), and immune cells to promote muscle hypertrophy and anti-inflammatory effects, resulting in maintaining skeletal muscle homeostasis. On the other hand, excessive exercise can induce inflammation and fibrosis in chronic inflammatory myopathy patients. In this study, we clarified a part of the mechanism of skeletal muscle regeneration and fibrosis induced by exercise by animal experiment. Furthermore, in vitro cell culture studies revealed that myoblasts and skeletal muscle-derived fibroblasts altered their response to mechanical stress depending on the inflammatory state.These findings might be contribute the development of effective and safe treatments for chronic myopathy patients.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
    Date (from‐to) : 2018/04 -2021/03 
    Author : Chikenji Takako
     
    Carpal tunnel syndrome (CTS) is the most common compression neuropathy. Effective treatments are limited to surgical interventions, and novel treatments have not yet been developed. In CTS patients, fibrosis of the subsynovial connective tissue surrounding the median nerve in the carpal tunnel is known as a pathological condition that compresses the median nerve in the carpal tunnel. In this study, we investigated cellular senescence in subsynovial connective tissue in CTS. In the CTS patients, PDGFRa + cells expressed both senescence factors and the macrophage checkpoint. PDGFRa + senescent cells avoided the phagocytosis of macrophages. Furthermore, PDGFRa+ senescent cells promoted the proliferation of adjacent fibroblasts. When senescent fibroblasts were transplanted into the carpal tunnel of a rabbit model, an increase in fibrosis-related genes was observed in the subsynovial connective tissue. Finally, one of senolytics induced apoptosis in PDGFRa + rather than control cells.
  • 自己骨髄間葉系幹細胞を用いた糖尿病合併症治療法の開発
    株式会社 ミネルヴァメディカ：
    Date (from‐to) : 2020/06 -2021 
    Author : 千見寺貴子
  • 糖尿病性腎症の自己骨髄間葉系幹細胞を用いた治療法の実用化研究
    株式会社 ミネルヴァメディカ：
    Date (from‐to) : 2019/11 -2020/05 
    Author : 千見寺貴子
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2016/04 -2019/03 
    Author : Saito Yuki, Chikenji Takako, Fujimiya Mineko
     
    Exercise-induced damage triggers muscle regeneration by activating satellite cells, fibro-adipogenic progenitors (FAPs), and immune cells. FAPs facilitated by exercise play roles for muscle remodeling. On the other hand, in the pathological conditions such as chronic inflammatory myopathy (CIM), exercise-induced damage rather exacerbates the inflammation and fibrosis in the muscle. Although it is considered that FAPs are key regulator for muscle inflammation and fibrosis, the cellular mechanisms are not completely understood. Here we aimed to demonstrate the mechanism for different roles of exercise-induced damage between normal and CIM by focusing on cell senescence in FAPs. we found that both of sufficient senescence and up-regulation of p38 MAPK, a pro-apoptotic signal, might be necessary for exercise-induced muscle regeneration. On the other hand, insufficient senescence and up-regulation of NF-ΚB, a anti-apoptotic signal might cause exercise-induced inflammation and fibrosis.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity start-up
    Date (from‐to) : 2016/08 -2018/03 
    Author : Matsumura Takashi, CHIKENJI Takako, SAITO Yuki, FUJIMIYA Mineko, OZASA Yasuhiro, SUZUKI Tomoyuki, TERAMOTO Atsushi, YAMASHITA Toshihiko
     
    Synovial tissue was collected from RA patient group, control group (knee ligament injury etc.), and immunohistological examination was conducted. Accumulation of phosphorylated platelet-derived growth factor receptor-αβ (pPDGFRαβ) positive FLS was observed in the synovial membrane of RA. Furthermore, the expression of Bcl-2 of apoptosis-resistant marker was elevated in pPDGFRαβ positive cells, p16 of cyclin-dependent kinase inhibitor was decreased. In RA synovium, it suggested that cell proliferation cannot be stopped, apoptosis is inhibited and cells might accumulate without apoptosis.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2013/04 -2017/03 
    Author : Chikenji Takako, SAITO Yuki, OZASA Yasuhiro, FUJIMIYA Mineko, IBA Kosuke
     
    An increased risk of carpal tunnel syndrome (CTS) has been reported in postmenopausal women. Fibrosis of subsynovial connective tissue (SSCT) is a prominent feature of CTS. Mesenchymal stem cells (MSCs) is considered to maintain the turnover of skeletal tissues in homeostasis. The purpose of this study was to assess the effect of the estradiol (E2) on SSCT-derived MSC (SSCT-MSC) of CTS patients in vitro. The proliferation activity of the SSCT-MSC was increased with E2. P16 mRNA expression and TP53 expression was significantly downregulated with E2. TGF-β was also downregulated with E2. We demonstrated that the effect of estradiol on SSCT-MSC in CTS patients, which increased the cell proliferation and inhibited the cellular senescence with downregulation of the TGF-β. Our findings suggested that the functional deterioration of SSCT-MSC might reside in the postmenopausal CTS patients, and relate to the homeostatic imbalance in SSCT fibrosis.
  • 細胞老化から解明するうつ病の脳内慢性炎症メカニズムと新規治療法の開発
    公益財団法人 秋山記念生命科学振興財団：公益財団法人 秋山記念生命科学振興財団 2022 年度 研究助成(一般)

Educational Activities

Teaching Experience

  • Seminar on Fundamental research for functional biology
    開講年度 : 2021
    課程区分 : 修士課程
    開講学部 : 保健科学院
    キーワード : 作業療法研究　基礎研究　リハビリテーション　神経解剖学　生体機能学
  • Fundamental research for functional biology
    開講年度 : 2021
    課程区分 : 修士課程
    開講学部 : 保健科学院
    キーワード : 作業療法研究　基礎研究　リハビリテーション　神経解剖学　生体機能学
  • Inter-Graduate School Classes(Educational Program):Health, Society and Environment
    開講年度 : 2021
    課程区分 : 修士課程
    開講学部 : 大学院共通科目
    キーワード : 健康科学，環境，人類生態，遺伝・細胞，代謝，ストレス応答，生体計測，健康情報，栄養、精神的健康
  • Experimental Methodology
    開講年度 : 2021
    課程区分 : 修士課程
    開講学部 : 保健科学院
    キーワード : 実験計画法，生物化学的研究方法，生理学的研究方法，分子・細胞生物学的研究方法，分析化学的研究方法，医用工学的研究方法，臨床医科学的研究方法，測定データ解析方法
  • Advanced Study of Rehabilitation Science
    開講年度 : 2021
    課程区分 : 博士後期課程
    開講学部 : 保健科学院
    キーワード : 理学療法、作業療法、精神障害、運動器障害、中枢神経障害
  • Advanced Seminar on Rehabilitation Science
    開講年度 : 2021
    課程区分 : 博士後期課程
    開講学部 : 保健科学院
    キーワード : 理学療法、作業療法、精神障害、運動器障害、中枢神経障害
  • Occupational Sciences and Skills
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 作業，健康，作業療法，作業分析，治療的応用
  • Introduction to Occupational Therapy
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 作業療法、健康、障害、作業、生活
  • Clinical Training Ⅳ
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 臨床実習，クリニカルクラークシップ
  • Clinical Training Ⅲ
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 臨床実習，クリニカルクラークシップ
  • Occupational Therapy for Physical Disabilities
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 身体障害，障害の特性，介入の目的，介入の意義，介入の方法
  • Clinical Training Ⅰ
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 臨床実習，クリニカルクラークシップ
  • Activities of Daily Living
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 日常生活活動（ADL），生活関連活動，評価，支援
  • Practice in Activities of Daily Living
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 日常生活活動（ADL），起居動作，移動，移乗，福祉用具，評価，支援方法，実技、喀痰等の吸引
  • Introduction to Health Sciences and Medicine
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 医学、医療、保健、福祉、医療制度、チーム医療、早期臨床体験実習
  • Evaluation Practice in Occupational Therapy for Physical Disabilities
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 身体障害，評価目的，評価法，評価技術，評価手法，実技
  • Introduction to Rehabilitation
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : リハビリテーション、障害、自立支援、就労支援、地域包括ケアシステム、理学療法、作業療法、
  • Clinical Training Ⅱ
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 臨床実習，クリニカルクラークシップ
  • Seminar of Research Methods in Occupational Therapy
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 作業療法学的研究、プレゼンテーション、論文執筆
  • Research Methods in Occupational Therapy
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 研究疑問、研究計画、研究法
  • Clinical practice in community based occupational therapy
    開講年度 : 2021
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 地域リハビリテーション, 地域作業療法, 地域ネットワーク

Social Contribution

Social Contribution

Media Coverage

  • 細胞老化の誘導機構を解明 慢性筋炎 骨格筋再生へ
    Date : 2020/03
    Writer: Other than myself
    Publisher, broadcasting station: 北海道医療新聞
    Paper
  • Combination of exercise and cell-aging drug could help people with chronic inflammatory myopathy
    Date : 2020/03
    Writer: Other than myself
    Publisher, broadcasting station: The Medical News
    Internet
  • Cells must age for muscles to regenerate in muscle-degenerating diseases
    Date : 2020/03
    Writer: Other than myself
    Publisher, broadcasting station: MedicalXpress
    Internet
  • 北海道大学、エクササイズが骨格筋にもたらす“再生“と”変性“～間葉系前駆細胞の“老化“は骨格筋の再生を促す! 細胞老化を味方にする新しい治療戦略への期待～
    Date : 2020/03
    Publisher, broadcasting station: 日経バイオテク
    Internet
  • 運動による「細胞の老化」が骨格筋の再生を促すことを発見
    Date : 2020/03
    Writer: Other than myself
    Program, newspaper magazine: 大学ジャーナル ON LINE
    Internet
  • SLEの抑うつ症状に老化細胞が関与、フィセチン経口投与で症状改善の可能性－
    http://www.qlifepro.com/news/20211201/sle-3.html Internet
  • 全身性エリテマトーデスの抑うつ症状に細胞老化が関与 ～老化細胞を標的とした新しい治療戦略への期待～
    Writer: Other than myself
    Publisher, broadcasting station: 日経バイオテク
    Internet

Others

  • 2022 死体解剖資格 (系統解剖)

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