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Kasai Hisae

Faculty of Fisheries Sciences　Marine Life Science　Marine Biotechnology and MicrobiologyProfessor

Researcher basic information

■ Degree
  • 博士（水産科学）, 北海道大学
■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research Keyword
  • 魚病
  • 魚類ウイルス
  • ゲノムワイド解析
  • 免疫
  • インターフェロン
  • ワクチン
  • 癌ウイルス
  • 抗ウイルス
  • 電解殺菌
  • 紫外線殺菌
  • 牡蠣
  • 腸炎ビブリオ
  • ノロウイルス
  • 浄化
Research Field
  • Life Science, Aquatic bioproduction science
■ Educational Organization

Career

■ Career
Career
  • Apr. 2024 - Present
    Hokkaido University, Faculty of Fisheries Sciences Division of Marine Life Science, 教授
Committee Memberships
  • Jun. 2025 - Present
    農林水産省, 獣医事審議会専門委員, Government
  • Apr. 2024 - Present
    厚生労働省, 薬事審議会臨時委員, Government
  • Apr. 2024 - Present
    農林水産省, 水産防疫専門家会議委員, Government
  • Feb. 2017 - Present
    北海道庁, 北海道栽培漁業推進協議会委員, Autonomy
  • Oct. 2016 - Present
    国際獣疫事務局 (WOAH), サケ科魚ヘルペスウイルス病リファレンスラボラトリー, Others
  • Jun. 2012 - May 2024
    北海道庁, 北海道渡島総合振興局大規模小売店舗立地審議会特別委員, Autonomy
  • Jan. 2019 - Apr. 2024
    厚生労働省, 薬事・食品衛生審議会臨時委員, Government
  • Oct. 2017 - Sep. 2023
    内閣府, 日本学術会議若手アカデミー会員, Government
  • Oct. 2017 - Sep. 2023
    内閣府, 日本学術会議連携会員, Government
  • Jan. 2015 - Jan. 2019
    厚生労働省, 薬事・食品衛生審議会専門委員, Government

Research activity information

■ Awards
  • Dec. 2024, 北海道大学, 桂田芳枝賞
    笠井久会
  • Mar. 2022, 第59回獣医疫学会学術集会優秀発表賞
    養殖における魚病疫学解析の問題とその解決法－実験と 観察を繋ぐ為の数理モデルの活用－
    大森亮介;松山亮太;降幡充;笠井久会
  • 2016, Hokkaido, A encouragement award of Science and Technology
    魚類増養殖ならびに漁獲物の衛生管理に資する海水殺菌法の確立
    Hisae Kasai
  • 2015, Japanese Society of Fisheries Science, A research encouragement award of Fisheries Science
    魚類防疫による生産安定化と漁獲物の衛生管理に関する研究
    Hisae Kasai
■ Papers
  • Validation of a dual-probe quantitative reverse transcription polymerase chain reaction for rapid detection of spring viremia of carp virus
    Ji-Yoon Park; Ji Su Kim; Hyoung Jun Kim; Euna Choi; Do-Hyung Kim; Dongbin Yun; Hisae Kasai; Jun Nagata; Tae Sung Jung; Se Ryun Kwon
    Aquaculture, 743131, 743131, Elsevier BV, Aug. 2025, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal
  • Fungicidal Activity of Glutaraldehyde against Causative Agents of Saprolegniasis in Fish Eggs: Saprolegnia parasitica, S. diclina, and S. hypogyna Strains
    Karn Tippayakraisri; Jun Nagata; Masautso E. Sakala; Keito Yamaguchi; Hisae Kasai
    Fish Pathology, 60, 2, 41, 49, The Japanese Society of Fish Pathology, 15 Jun. 2025, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Immunoglobulin gene expression as a biomarker for Streptococcosis resistance in Nile tilapia (Oreochromis niloticus).
    Suwit Wuthisuthimethavee; Thanakorn Khunrang; Chettupon Pooljun; Hisae Kasai
    Fish & shellfish immunology, 159, 110199, 110199, Apr. 2025, [Peer-reviewed], [Last author], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal, Streptococcus agalactiae is a significant pathogen in Nile tilapia (Oreochromis niloticus) culture, causing economic losses in Thailand. Formalin-killed S. agalactiae vaccine in tilapia can be used in the prevention of diseases and mortalities. In this study, we administered a formalin-killed S. agalactiae vaccine to individual tilapia broodstock to activate immunoglobulin (IgM) gene expression. The IgM gene expression levels differed among individual broodstock and were grouped into four categories: high IgM males (HM), low IgM males (LM), high IgM females (HF), and low IgM females (LF). Using these categories, we designed matings to create four breeding groups: HM × HF, HM × LF, LM × HF, and LM × LF, generating three fry families per group. The tilapia fry was then orally challenged with S. agalactiae for 14 days. The results showed a significant difference (p < 0.05) in survival rates among the breeding groups. The HM × HF group had the highest survival at 93.33 ± 6.20 %, while the LM × LF group had the lowest at 21.11 ± 6.50 %. Gene expression analysis of toll-like receptor 2, hepcidin, immunoglobulin, MHC class II, and granzyme genes at 24, 48, 72, and 96 h post S. agalactiae challenge revealed significant temporal changes. IgM and granzyme expression levels varied notably among groups, with IgM peaking at 72-96 h and showing a significant difference (p < 0.05) between groups. The HM × HF, HM × LF, and LM × HF breeding groups displayed significantly higher IgM expression levels compared to the LM × LF group. Granzyme gene expression also showed significant differences (p < 0.05) from 48 h post-challenge, with the highest expression observed in the HM × HF group and the lowest in the LM × LF group. No significant difference (p > 0.05) was observed between the HM × LF and LM × HF groups for granzyme expression. This experiment concludes that high levels of IgM gene expression of broodstock tilapia induced by formalin-killed S. agalactiae vaccine could be used as a gene marker to select the S. agalactiae-resistant tilapia in breeding program.
  • Efficacy of Egg Disinfection by Povidone-Iodine Solutions for Inactivation of Causative Agent of Bacterial Kidney Disease in vitro
    Jun Nagata; Sayo Morimoto; Makoto Ameno; Keito Yamaguchi; Ken-ichi Watanabe; Dominic K. Bagenda; Hisae Kasai
    Fish Pathology, 59, 3, 91, 94, The Japanese Society of Fish Pathology, 15 Sep. 2024, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Long-term detection of cyprinid herpesvirus 1 genome and tumorous transcriptome in common carp (Cyprinus carpio) infected with the virus
    Jun Nagata; Katsuki Takita; Hisae Kasai
    Aquaculture, 741158, 741158, Elsevier BV, Jun. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal
  • Pathogenicity and tumorigenicity of Oncorhynchus masou virus (OMV) isolated from cultured coho salmon Oncorhynchus kisutch in Hokkaido, Japan
    勝又義友; 勝又義友; 水野伸也; 永田淳; 笠井久会
    水産増殖, 71, 4, 2023, [Peer-reviewed], [Last author], [Domestic magazines]
    Japanese, Scientific journal
  • Genome taxonomy of the genus Neptuniibacter and proposal of Neptuniibacter victor sp. nov. isolated from sea cucumber larvae.
    Rika Kudo; Ryota Yamano; Juanwen Yu; Shotaro Koike; Alfabetian Harjuno Condro Haditomo; Mayanne A M de Freitas; Jiro Tsuchiya; Sayaka Mino; Fabiano Thompson; Jesús L Romalde; Hisae Kasai; Yuichi Sakai; Tomoo Sawabe
    PloS one, 18, 8, e0290060, 2023, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal, A Gram-staining-negative, oxidase-positive, strictly aerobic rod-shaped bacterium, designated strain PT1T, was isolated from the laboratory-reared larvae of the sea cucumber Apostichopus japonicus. A phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PT1T was closely related to Neptuniibacter marinus ATR 1.1T (= CECT 8938T = DSM 100783T) and Neptuniibacter caesariensis MED92T (= CECT 7075T = CCUG 52065T) showing 98.2% and 98.1% sequence similarity, respectively. However, the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values among these three strains were 72.0%-74.8% and 18.3%-19.5% among related Neptuniibacter species, which were below 95% and 70%, respectively, confirming the novel status of PT1T. The average amino acid identity (AAI) values of PT1T showing 74-77% among those strains indicated PT1T is a new species in the genus Neptuniibacter. Based on the genome-based taxonomic approach, Neptuniibacter victor sp. nov. is proposed for PT1T. The type strain is PT1T (JCM 35563T = LMG 32868T).
  • The pan-genome of Splendidus clade species in the family Vibrionaceae: insights into evolution, adaptation, and pathogenicity.
    Chunqi Jiang; Hisae Kasai; Sayaka Mino; Jesús L Romalde; Tomoo Sawabe
    Environmental microbiology, 24, 10, 4587, 4606, 15 Sep. 2022, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal, The Splendidus clade is the largest clade in Vibrionaceae, and its members are often related to mortality of marine animals with huge economic losses. The molecular bases of their pathogenicity and virulence, however, remain largely unknown. In particular, the complete genome sequences of the Splendidus clade species are rarely registered, which is one of the obstacles to predict core and/or unique genes responsible to their adaptation and pathogenicity, and to perform a fine scale meta-transcriptome during bacterial infection to their hosts. In this study, we obtained the complete genomes of all type strains in the Splendidus clade and revealed that 1) different genome sizes (4.4-5.9 Mb) with V. lentus the biggest and most of them had several big plasmids, likely because of the different features on mobilome elements, 2) the Splendidus clade consists of 19 species except V. cortegadensis, and 3 sub-clades (SC) were defined with the 15 most closely related members as SC1; 3) different carbohydrate degradation preferences may be the result of environmental adaptation, 4) a broad prediction of virulence factors (VFs) revealed core and species unique VF genes. This article is protected by copyright. All rights reserved.
  • Estimating the comprehensive overview of Oncorhynchus masou virus disease outbreak in Rainbow trout using mortality data: Coupling data on experimental infection and field observation by mathematical modeling
    Ryosuke Omori; Ryota Matsuyama; Yukihiko Nakata; Mitsuru Furihata; Hisae Kasai
    Aquaculture, 554, 738165, 738165, Elsevier BV, May 2022, [Peer-reviewed], [Last author], [International Magazine]
    English, Scientific journal
  • Light Response of Vibrio parahaemolyticus
    Yunato Kuroyanagi; Jiro Tsuchiya; Chunqi Jiang; Sayaka Mino; Hisae Kasai; Daisuke Motooka; Tetsuya Iida; Masataka Satomi; Tomoo Sawabe
    Frontiers in Marine Science, 9, Frontiers Media SA, 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Light is one of the most critical stimuli in the majority of living organisms. In the last two decades, blue light (BL) has become a major subject of attention because of developments in light-emitting diodes (LED). The effects of BL on eukaryotic organisms and phototrophic prokaryotes have been well studied, but the knowledge of its effects on non-phototrophic prokaryotes remains unclear. Since BL can penetrate seawater, it is expected that most prokaryotes living in the ocean possess molecular mechanisms which protect against BL. The aim of this study is to assess the molecular mechanisms of Vibrio parahaemolyticus cells against BL as a marine bacterial model compared to other wavelength light exposures. Physiological and transcriptomic analyses of BL-exposed cells compared to other light treated cells revealed the highest ROS fold change, the highest number of differentially expressed genes (DEGs), and up-regulation in the gene responsible to not only compatible solute such as glycine betaine and ectoine but also iron-sulfur biosynthesis related to ROS formation. Furthermore, red light (RL) up-regulated the expression of cryptochrome DASH, a protein known to be excited by BL, and orange light (OL) decreased the expression of thermostable direct hemolysin (TDH), suggesting that OL attenuates the virulence of V. parahaemolyticus. In addition, the expression of VtrA (V. parahaemolyticus type III secretion system 2 (T3SS2) regulator A) but not VtrB (V. parahaemolyticus T3SS2 regulator B) increased under both light treatments, indicating that light exposure is unlikely to be involved in T3SS2-mediated pathogenicity. These results expand our knowledge on unique light responses in non-phototrophic marine prokaryotes.
  • Functional and Stress Response Analysis of Heat Shock Proteins 40 and 90 of Giant River Prawn (Macrobrachium rosenbergii) under Temperature and Pathogenic Bacterial Exposure Stimuli.
    Tanya Ju-Ngam; Nichanun McMillan; Mamoru Yoshimizu; Hisae Kasai; Ratree Wongpanya; Prapansak Srisapoome
    Biomolecules, 11, 7, 15 Jul. 2021, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal, The aims of this research were to perform molecular characterization and biofunctional analyses of giant river prawn Hsp40 and Hsp90 genes (Mr-hsp40 and Mr-hsp90) under various stress conditions. Comparisons of the nucleotide and amino acid sequences of Mr-hsp40 and Mr-hsp90 with those of other species showed the highest similarity scores with crustaceans. Under normal conditions, expression analysis using quantitative real-time RT-PCR (qRT-PCR) indicated that Mr-hsp40 was highly expressed in the gills and testis, and Mr-hsp90 expression was observed in all tissues, with the highest expression in the ovary. The expression patterns of Mr-hsp40 and Mr-hsp90 transcripts under Aeromonas hydrophila challenge and heat-cold shock conditions were examined in gills, the hepatopancreas and hemocytes, at 0, 3, 6, 12, 24, 48 and 96 h by qRT-PCR. Under bacterial challenge, Mr-hsp40 displayed variable expression patterns in all tissues examined during the tested periods. In contrast, upregulated expression of Mr-hsp90 was quickly observed from 3 to 12 h in the gills and hepatopancreas, whereas obviously significant upregulation of Mr-hsp90 was observed in hemocytes at 12-96 h. Under temperature shock conditions, upregulation of Mr-hsp40 expression was detected in all tested tissues, while Mr-hsp90 expression was quickly upregulated at 3-48 h in all tissues in response to 35 °C conditions, and conditions of 35 and 25 °C stimulated its expression in gills and the hepatopancreas at 12 and 48 h, respectively. Silencing analyses of these two genes were successfully conducted under normal, high-temperature (35 °C) and A. hydrophila infection conditions. Overall, knockdown of Mr-hsp40 and Mr-hsp90 effectively induced more rapid and higher mortality than in the PBS control and GFP induction groups in temperature and infectious treatments. Evidence from this study clearly demonstrated the significant functional roles of Mr-hsp40 and Mr-hsp90, which are crucially involved in cellular stress responses to both temperature and pathogenic bacterial stimuli.
  • Hygiene Practices for Aquaculture and Hatchery Management
    笠井久会; 永田淳; 永田淳
    魚病研究, 55, 4, 2021, [Invited], [Lead author, Corresponding author], [Domestic magazines]
  • Influence of the Student Handbook in Program Enrolment of Female Students in the Faculty of Fisheries Sciences, Hokkaido University
    Hisae Kasai; Katsutoshi Arai
    Bulletin of Fisheries Sciences, Hokkaido University, 70, 2, 113, 118, 23 Dec. 2020, [Lead author], [Domestic magazines]
    Japanese, Research institution
  • Efficacy of commercial curing processes for treatment of quarantinable disease-causing pathogens in salmon roe
    Jun Nagata; Sayo Morimoto; Dominic K. Bagenda; Hisae Kasai
    Fisheries Science, 86, 5, 829, 834, [Springer Japan], 01 Sep. 2020, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Changes in the physicochemical properties of fish cell membranes during cellular senescence
    Akifumi Shiomi; Kohjiro Nagao; Hisae Kasai; Yuji Hara; Masato Umeda
    Bioscience, Biotechnology and Biochemistry, 84, 3, 583, 593, 03 Mar. 2020, [Peer-reviewed], [Domestic magazines]
    English, Scientific journal
  • Vibriosis in Fish: A Review on Disease Development and Prevention
    M. Y. Ina-Salwany; Nurhidayu Al-saari; Aslah Mohamad; Fathin Amirah Mursidi; Aslizah Mohd-Aris; M. N.A. Amal; Hisae Kasai; Sayaka Mino; Tomoo Sawabe; M. Zamri-Saad
    Journal of Aquatic Animal Health, 31, 1, 3, 22, Mar. 2019, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal
  • Impact of feeding on oyster depuration efficacy under conditions of high salinity and low temperature
    Dominic K. Bagenda; Shotaro Nishikawa; Hiroki Kita; Yu Kinai; Shima Terai; Motoichi Kato; Hisae Kasai
    Aquaculture, 500, 135, 140, 01 Feb. 2019, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    Scientific journal
  • Factors affecting tolerance and immunoreactivity of rainbow trout (Oncorhynchus mykiss) to Polyinosinic-polycytidylic acid
    Shotaro Nishikawa; Tetsuo Nakano; Hisae Kasai
    Aquaculture Science, 67, 1, 25, 32, Japanese Society for Aquaculture Science, 2019, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Observation of the Micropyle of Unfertilized and Water-Hardening Rainbow Trout Eggs by Scanning Electron Microscopy
    小原 昌和; 野村 哲一; 笠井 久会; 吉水 守
    Fish Pathology, 53, 2, 90, 93, The Japanese Society of Fish Pathology, 2018, [Domestic magazines]
    Japanese,

    吸水過程において卵門から細菌性冷水病や細菌性腎臓病の病原細菌が卵内へ侵入する卵内感染の機序を明らかにするため，SEMを使用して，ニジマスの未受精卵，吸水卵および人為的な病原菌汚染卵における卵門および卵膜の形態を観察した。排卵された未受精卵の卵膜表面では，卵門を除き細菌が卵内に侵入する可能性のある小孔状の形態はみられなかった。卵門管の上部および管底の口径は細菌が十分に通過できるほどに大きく，吸水に伴って管内の閉塞が進む時期は実験的に卵内感染が成立した時期と一致することが確かめられた。

  • Occurrence and prevention of injured bacterial cells in fisheries
    Koji Yamazaki; Kantaro Takeuchi; Yohei Yamazaki; Sayaka Mino; Hisae Kasai; Toko Sawabe; Tomoo Sawabe; Masataka Satomi
    Nippon Shokuhin Kagaku Kogaku Kaishi, 65, 4, 197, 204, Japanese Society for Food Science and Technology, 2018, [Domestic magazines]
    Japanese
  • Pathogenicity of a rainbow trout isolate rtna-0010 of oncorhynchus masou virus (salmonid herpesvirus 2) in salmonid and cyprinid fish
    Keisuke Ikemoto; Mamoru Yoshimizu; Mitsuru Furihata; Masakazu Kohara; Hisae Kasai
    Fish Pathology, 51, 2, 60, 63, 2016, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Identification and evaluation of the pathogenicity of a scuticociliate isolated from diseased barfin flounder Verasper moseri and sensitivity of a pathogen isolate to vinegar and tea extract solutions in sea water
    Ito Shingo; Kasai Hisae
    Aquaculture Science, 63, 3, 255, 259, Japanese Society for Aquaculture Science, 2015, [Peer-reviewed], [Domestic magazines]
    English, We observed mortality due to scuticociliate infection in barfin flounder and subsequently identified the causative pathogen and examined its pathogenicity. In addition, we examined the sensitivity of a pathogen isolate and the tolerance of the barfin flounder to vinegar solutions and tea extract solutions to identify scuticociliate infection control measures in this fish species. Species identification of the causative agent was performed using polymerase chain reaction (PCR); the causative agent was identified as Miamiensis avidus. M. avidus was administered to barfin flounder via immersion or intraperitoneal injection. In the intraperitoneal injection experiments, the cumulative mortality rate within one week was 100% at a dose of 105 ciliates/fish. In contrast, immersion challenge experiments were unsuccessful. M. avidus survival fell below the detection limit after exposure to a 0.7% vinegar solution or a 0.01% tea extract solution for 30 min. Exposure to a 0.03% tea extract solution for 1h was toxic to the barfin flounder, whereas exposure to a 0.7% vinegar solution for 1h exhibited low toxicity. In conclusion, we suggest that exposure to a 0.7% vinegar solution for 30 min is an effective M. avidus infection treatment and control method in vitro.
  • The importance of detection limits in the diagnosis of asymptomatic furunculosis
    Devon Ronald Dublín; Mamoru Yoshimizu; Hisae Kasai
    Revista Electronica de Veterinaria, 15, 6, 1, 11, Veterinaria Organización, 01 Jun. 2014, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    Spanish, Castilian, Scientific journal
  • Approaches to Probiotics for Aquaculture:—Prevention of Fish Viral Diseases Using Antiviral Intestinal Bacteria—
    YOSHIMIZU Mamoru; KASAI Hisae; WATANABE Kenichi
    Journal of Intestinal Microbiology, 28, 1, 7, 14, JAPAN BIFIDUS FOUNDATION, 2014, [Peer-reviewed], [Domestic magazines]
    Japanese, Generally, normal bacterial microbiota play an important role in inhibiting the growth of pathogenic bacteria in the intestine or on the skin, and stimulate the immune responses of host animals. In aquaculture, mortalities of larvae and fry of cultured fish due to viral diseases remain a major problem for regular food production. Although vaccines can be useful to control viral diseases, because of a immature immune system, they are not effective during the early stages of larval growth. Therefore alternative strategies to control viral diseases are required for sustainable aquaculture and seed production. We investigated fish pathogenic viruses in an aquatic environment in order to understand their survival and interactional relationships with bacteria for the prevention of fish viral diseases in aquaculture. In a series of studies of the microbial ecosystem, we found that many bacteriaproducing antiviral substances could be isolated from aquatic environments, such as hatchery water supply, river mouth water, beach water, and intestinal contents of aquatic animals. Intestinal bacteria, such as Aeromonas spp. and Vibrio spp. producing anti-viral substances were isolated from intestinal contents of masu salmon (Oncorhynchus masou), Japanese flounder (Paralichthys olivaceus), barfin flounder (Verasper moseri) and Pacific oyster (Crassostrea gigas). Aeromonas strains produced anti-infectious hematopoietic necrosis (IHNV; Novirhabdovirus) substances, and Vibrio strains showed anti-IHNV, salmonid herpesvirus Oncorhynchus masou virus (OMV; Salmonivirus), hirame rhabdovirus (HIRRV), barfin flounder nervous necrosis virus (BF-NNV; Nodavirus) and feline calicivirus (FCV) activities. When A. hydrophila strains M-26 and M-38 were mixed with food pellets and fed to rainbow trout (O. mykiss) and masu salmon, both bacteria became dominant in the intestinal microbiota and anti-IHNV activity was observed in homogenates of intestinal contents. These rainbow trout and masu salmon fed the Aeromonas spp. showed more resistance to artificial IHNV challenge tests. Barfin flounder fed Vibrio spp. strains 2IF6 with Altemia sp. showed anti-IHNV, OMV, HIRRV and BF-NNV activities in their intestinal contents. Larvae fed the Vibrio spp. showed a higher survival rate than the control fish. In the case of Japanese flounder larvae fed with V. alginolyticus strain V-23 manipulated rotifer showed anti-OMV (FHV; flounder herpesvirus surrogate) activities in the intestinal contents and rearing water in the tank. Furthermore, V. neptunis strain V-176 and Vibrio sp. Strain V-4 isolated from intestinal contents of Pacific oyster showed anti-feline calicivirus (FCV; Norovirus surrogate) and OMV (Ostreid herpesvirus-1 microvariant (OsHV-1 μVar) surrogate) activities. Norovirus and OsHV-1μVar will hopefully be inactivated in the intestines of oysters fed V. neptunis V-176 or Vibrio sp. V-4 with Chaetoceros gracilis, respectively. These results show that, by manipulating diets with anti-viral substance-producing bacteria, the resistance of fish or shellfish larvae to viral disease can be improved thereby helping to ensure regular food production through aquaculture.
  • Inactivation of koi-herpesvirus in water using bacteria isolated from carp intestines and carp habitats
    N. Yoshida; R. K. Sasaki; H. Kasai; M. Yoshimizu
    Journal of Fish Diseases, 36, 12, 997, 1005, Dec. 2013, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Scientific journal
  • Mos1 transposon-based transformation of fish cell lines using baculoviral vectors
    Masako Yokoo; Ryosuke Fujita; Yumiko Nakajima; Mamoru Yoshimizu; Hisae Kasai; Shin ichiro Asano; Hisanori Bando
    Biochemical and Biophysical Research Communications, 439, 1, 18, 22, 1, 13 Sep. 2013, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Low possibility of intra-ovum infection with Flavobacterium osychrophilum or Renibacterium salmoninarum in the Salmonid coelomic cavity
    Masakazu Kohara; Hisae Kasai; Mamoru Yoshimizu
    Fish Pathology, 48, 3, 97, 100, 2013, [Peer-reviewed], [Domestic magazines]
    English, Scientific journal
  • Elimination of Escherichia coli from oysters using sodium bicarbonate
    Matthura Labaiden; Hisae Kasai; Mamoru Yoshimizu; Sataporn Direkbusrakom
    Walailak Journal of Science and Technology, 10, 6, 601, 606, 2013, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal
  • Intra-ovum infection in salmonid eggs artificially contaminated with fish pathogenic bacteria: Flavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida
    Masakazu Kohara; Hisae Kasai; Mamoru Yoshimizu
    Fish Pathology, 47, 2, 49, 55, 2012, [Peer-reviewed], [Domestic magazines]
    English, Scientific journal
  • Elimination of Escherichia coli from oysters using electrolyzed seawater
    Hisae Kasai; Koji Kawana; Matthura Labaiden; Kenji Namba; Mamoru Yoshimizu
    Aquaculture, 319, 3-4, 315, 318, 01 Oct. 2011, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
    English, Scientific journal
  • Distribution of coli forms and Escherichia coli at fishing ports
    Jun Yokoyama; Hisae Kasai; Atsumi Furuya; Mamoru Yoshimizu
    Nippon Suisan Gakkaishi (Japanese Edition), 77, 3, 409, 415, 2011, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • Absorbability of bacteria isolated from active sludge of fish rearing waste water treatment facilities
    Natsuko Yoshida; Kazuaki Ishida; Hisae Kasai; Mamoru Yoshimizu
    Nippon Suisan Gakkaishi (Japanese Edition), 77, 1, 94, 96, 2011, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • Effect of Egg-washing with Isotonic Solution on Elimination of Bacteria and Viruses before Fertilization in Salmon Aquaculture
    KOHARA Masakazu; OGAWA Shigeru; KASAI Hisae; YOSHIMIZU Mamoru
    Aquaculture Science, 58, 1, 37, 43, Japanese Society for Aquaculture Science, 20 Mar. 2010, [Peer-reviewed], [Domestic magazines]
    Japanese, Egg-washing with isotonic solution has been used to improve the artificial fertilization in fresh-water salmon aquaculture.Usually, unfertilized eggs are rinsed twice or three times with isotonic solution and showered before fertilization. We investigated the effect of egg-washing using rainbow trout eggs on elimination of some causative agents of cold water disease (Flavobacterium psychrophilum), flunculosis (Aeromonas salmonicida), infectious hematopoietic necrosis (IHNV) and Oncorhynchus masou virus disease (OMV), quantitatively and on prevention of intra-ovum infection with F. psychrophilum. The number of live bacterial cells or virus infectivity titers which were eliminated by egg-washing, was 103CFU/ml in F. psychrophilum, 105CFU/ml in A. salmonicida, or 104TCID50/ml in both of IHNV and OMV. In shower washing, the eliminated extent of F. psychrophilum or A. salmonicida cells increased with increasing isotonic solution volume. The ratio of intra-ovum infection with F. psychrophilum was clearly less in rinsed eggs than in non-treated eggs, and result of detection from showered eggs was negative. These results suggest that egg-washing with isotonic solution is very effective to eliminate the bacteria or viruses on egg surface and is able to decrease the risk of intra-ovum infection.
  • Bacteriological observation of fishing port for hygiene control
    Jun Yokoyama; Hisae Kasai; Satomi Mori; Hiroshi Hayashi; Mamoru Yoshimizu
    Nippon Suisan Gakkaishi (Japanese Edition), 76, 1, 62, 67, 2010, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • Virulence change of infectious hematopoietic necrosis virus against rainbow trout oncorhynchus mykiss with viral molecular evolution
    Mamiko Mochizuki; Hyoung Jun Kim; Hisae Kasai; Toyohiko Nishizawa; Mamoru Yoshimizu
    Fish Pathology, 44, 4, 159, 165, Dec. 2009, [Peer-reviewed], [Domestic magazines]
    English, Scientific journal
  • Surveillance of salmonid viruses especially targeting infectious salmon anemia virus in Japan
    Hisae Kasai; Shuhei Iwawaki; Mamoru Yoshimizu
    Fish Pathology, 44, 4, 182, 184, Dec. 2009, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • JNK and p38 MAPK are independently involved in tributyltin-mediated cell death in rainbow trout (Oncorhynchus mykiss) RTG-2 cells
    Noriko Urushibara; Shinya Mitsuhashi; Tomoyuki Sasaki; Hisae Kasai; Mamoru Yoshimizu; Hiroyoshi Fujita; Atsushi Oda
    Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 149, 4, 468, 475, May 2009, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Teleost TLR22 recognizes RNA duplex to induce IFN and protect cells from birnaviruses
    Aya Matsuo; Hiroyuki Oshiumi; Tadayuki Tsujita; Hiroshi Mitani; Hisae Kasai; Mamoru Yoshimizu; Misako Matsumoto; Tsukasa Seya
    Journal of Immunology, 181, 5, 3474, 3485, 01 Sep. 2008, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Survival of koi herpesvirus (KHV) in environmental water
    Tomoko Shimizu; Natsuko Yoshida; Hisae Kasai; Mamoru Yoshimizu
    Fish Pathology, 41, 4, 153, 157, Dec. 2006, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Effect of rearing using an electrolyzed seawater on reduction of Vibrio parahaemolyticus from sea urchin
    Minoru Kimura; Kanako Mikami; Hiroshi Hoshikawa; Tatsunari Mori; Hisae Kasai; Mamoru Yoshimizu
    Nippon Suisan Gakkaishi (Japanese Edition), 72, 1, 1, 5, Jan. 2006, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • Effect of Manipulation of Dietary Rotifer Bacterial Flora on the Intestinal Bacterial Flora of Japanese Flounder
    SHIMIZU Tomoko; SHINOZAKI Daisuke; KASAI Hisae; SAWABE Tomoo; WATANABE Kenichi; YOSHIMIZU Mamoru
    Aquaculture Science, 53, 3, 275, 278, Japan Aquaculture Society, 20 Sep. 2005, [Peer-reviewed], [Domestic magazines]
    Japanese, Effect of manipulation of dietary rotifer Brachionus plicatilis bacterial flora on the intestinal bacterial flora of Japanese flounder Paralichthys olivaceus larvae was investigated. Manipulation was done by disinfecting rotifer eggs to reduce viable bacterial counts and then by inoculating hatched rotifers with non-sucrose fermenting Vibrio splendidus strain V-15 to make the bacterium dominant in the rotifers. Flounder larvae were fed the manipulated rotifers and intestinal bacterial flora of the fish was monitored. The dominant intestinal bacterial flora of the flounder became Vibrio spp. These results indicate that intestinal bacterial flora of flounder can be artificially controlled by manipulating bacterial flora in diets.
  • Virucidal effects of ultraviolet, heat treatment and disinfectants against koi herpesvirus (KHV)
    Hisae Kasai; Yoshifumi Muto; Mamoru Yoshimizu
    Fish Pathology, 40, 3, 137, 138, Sep. 2005, [Peer-reviewed], [Lead author], [Domestic magazines]
    English
  • Virus surveillance of wild marine fish collected in coastal areas of Hokkaido, Japan
    Kenichiro Kobayashi; Jacob A. Wani; Hisae Kasai; Toyohiko Nishizawa; Mamoru Yoshimizu
    Fish Pathology, 40, 1, 29, 31, Mar. 2005, [Peer-reviewed], [Domestic magazines]
    English, Scientific journal
  • Survivability of Fish Pathogenic Viruses in Environmental Water, and Inactivation of Fish Viruses (Special Issue: International Symposium on Koi Herpesvirus disease)
    Yoshimizu Mamoru; Yoshinaka Toko; Hatori Shuichi; Kasai Hisae
    Bulletin of Fisheries Research Agency, 0, 47, 54, Fisheries Research Agency, Mar. 2005, [Last author], [Domestic magazines]
    English, Special Issue: International Symposium on Koi Herpesvirus Disease : Strategy for Koi Herpesvirus Disease Control. 13 March 2004. Yokohama, Japan.
  • 秋サケの食品としての安全性確保について
    笠井 久会; 野村 哲一; 吉水 守
    魚と卵 : さけ・ます資源管理センター技術情報, 170, 170, 1, 8, さけ・ます資源管理センター, Mar. 2004, [Lead author], [Domestic magazines]
    Japanese
  • Observations of bacteria collected at hygienically controlled Shibetsu fishing port, Japan
    Hisae Kasai; Emi Sugiyama; Mamoru Yoshimizu
    Nippon Suisan Gakkaishi (Japanese Edition), 70, 1, 60, 65+98, Jan. 2004, [Peer-reviewed], [Lead author], [Domestic magazines]
    Japanese, Scientific journal
  • Disinfection of seawater from fishing ports by an electrolytic apparatus and its application to fisheries sanitation
    Hisae Kasai; Mamoru Yoshimizu
    Nippon Suisan Gakkaishi (Japanese Edition), 69, 6, 955, 959, Nov. 2003, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • さけ・ます増殖事業における防疫対策
    野村哲一; 笠井久会
    魚と卵, 169, 169, 1, 12, Mar. 2003, [Domestic magazines]
    Japanese, Research institution
  • Prevention of scuticociliatosis in Japanese flounder by treatment of water-supply with a high quality UV lamp
    Hisae Kasai; Shuichi Osawa; Tadashi Kobayashi; Mamoru Yoshimizu
    Fish Pathology, 37, 4, 199, 200, Dec. 2002, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • Observation on Bacteria collected at Sawara Fishing Port, Hokkaido, Japan
    笠井 久会; 中村 哲士; 吉水 守
    Bulletin of fisheries sciences, Hokkaido University, 53, 2, 69, 73, GRADUATE SCHOOL OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY, Aug. 2002, [Lead author], [Domestic magazines]
    Japanese
  • Observations on Bacteria Collected in the Shibetsu, Ichani and Kotanuka Rivers in Eastern Hokkaido, Japan
    笠井 久会; 中村 暢之; 吉水 守
    Bulletin of fisheries sciences, Hokkaido University, 53, 2, 75, 82, GRADUATE SCHOOL OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY, Aug. 2002, [Lead author], [Domestic magazines]
    Japanese
  • 種苗生産施設における用水及び排水の殺菌
    吉水 守; 笠井 久会
    工業用水, 0, 523, 13, 26, 日本工業用水協会, Apr. 2002, [Last author], [Domestic magazines]
    Japanese, 総説
  • Isolation of Aeromonas salmonicida, Causative Agent of Furunculosis, from Chum Salmon Caught in the River or Coast
    NOMURA Testsuici; HONMA Hiromi; KASAI Hisae; YOSHIMIZU Mamoru
    Bulletin of fisheries sciences, Hokkaido University, 53, 1, 45, 50, GRADUATE SCHOOL OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY, Mar. 2002, [Domestic magazines]
    Japanese
  • DISINFECTION OF WATER FOR AQUACULTURE
    KASAI HISAE; YOSHIMIZU MAMORU; EZURA YOSHIO
    Fisheries science, 68, 1, 821, 824, 2002, [Peer-reviewed], [Lead author], [Domestic magazines]
    English
  • Establishment of Two Japanese Flounder Embryo Cell Lines
    KASAI Hisae; YOSHIMIZU Mamoru
    Bulletin of fisheries sciences, Hokkaido University, 52, 2, 67, 70, GRADUATE SCHOOL OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY, Sep. 2001, [Lead author], [Domestic magazines]
    Japanese
  • Disinfectant Effects of Hypochlorite Produced by Batch Electrolytic System on Fish Pathogenic Bacteria and Virus
    KASAI Hisae; WATANABE Ken-ichi; YOSHIMIZU Mamoru
    Aquaculture Science, 49, 2, 237, 241, Japan Aquaculture Society, 20 Jun. 2001, [Peer-reviewed], [Lead author], [Domestic magazines]
    Japanese, The bactericidal and virucidal effects of hypochlorite produced by batch electrolytic system were examined for fish pathogens. Sodium chloride solutions, ranging from 0.5 to 3%, were electrolyzed and the concentration of chlorine produced was measured. Almost same concentration of chlorine was produced when 1.0% or more NaCI solution and seawater were electrolyzed. Fish pathogenic bacteria or virus was added to electrolyzed water and exposed to hypochlorite. More than 99% cells of Vibrio anguillarum, causative agent of fish vibriosis, were killed when the bacteria were exposed to 0.21mg/l hypochlorite for 1min. Yellowtail ascites virus (YAV) and hirame rhabdovirus (HIRRV), the causative agents of viral ascites disease and rhabdovirus disease of marine fish, were inactivated more than 99% after treatment with 0.42 mg/l hypochlorite for 1 min. The number of viable bacteria in the hatchery supply-seawater was reduced more than 99% when the water was treated with hypochlorite of 0.54 mg/l for 1 min. And more than 99% cells of viable bacteria in the waste-seawater were killed when the water was treated with hypochlorite of 0.64 mg/l for 5min. The batch system developed in this study revealed remarkable bactericidal and virucidal effects, which were very similar to those in continuous flow-water system.
  • Bactericidal effect of continuous flow electrolyzer on hatchery waste-seawater
    Hisae Kasai; Ken Ichi Watanabe; Mamoru Yoshimizu
    Nippon Suisan Gakkaishi (Japanese Edition), 67, 2, 222, 225, Mar. 2001, [Peer-reviewed], [Lead author], [Domestic magazines]
    Japanese, Scientific journal
  • Disinfectant effects of electrolyzed salt water on fish pathogenic bacteria and viruses
    Hisae Kasai; Asami Ishikawa; Yuhka Hori; Ken Ichi Watanabe; Mamoru Yoshimizu
    Nippon Suisan Gakkaishi (Japanese Edition), 66, 6, 1020, 1025, 2000, [Peer-reviewed], [Lead author], [Domestic magazines]
    Japanese, Scientific journal
■ Other Activities and Achievements
■ Books and other publications
  • 循環式陸上養殖Vol.2
    笠井久会; 永田; 淳, 62-68
    緑書房, Jun. 2024, 9784895319874, [Contributor]
  • Seafood safety and quality
    Kasai, H; S. Nishikawa, Prevention and treatment of diseases caused by fish pathogens
    CRC press, 22 Oct. 2018, [Contributor]
  • Seafood safety and quality
    Kasai, H; S. Nishikawa; K. Watanabe, Viral diseases affecting aquaculture
    CRC press, 22 Oct. 2018, [Contributor]
  • Fish viruses and bacteria : pathobiology and protection
    Woo P. T. K; Cipriano Rocco C, Oncogenic Viruses - Oncorhynchus masou Virus and Cyprinid Herpesvirus
    CAB International, 2017, 9781780647784, English, [Contributor]
  • 魚介類の微生物感染症の治療と予防
    青木 宙; 飯田貴次; 中西照幸; 引間順一; 廣野育生; 舞田正志; 吉水 守; 伊丹利明; 上野隆二; 遠藤俊夫; 乙竹 充; 笠井久会; 近藤昌和; 酒井正博; 高橋幸則; 青木 宙
    恒星社厚生閣, 23 Aug. 2013, 4769912811, 504, [Joint work]
  • Viral, Bacterial and Fungal Infections
    Woo P. T. K; Bruno David W, Chapter 7; Oncogenic viruses and Oncorhyncus masou virus
    CABI, 2011, 9781845935542, English, [Contributor]
  • サケ学入門―自然史・水産・文化
    阿部 周一; 天野哲也; 荒井克俊; 安東宏徳; 上田 宏; 浦野明宏; 帰山雅秀; 笠井久会; かじ さやか; 桜井泰憲; 佐藤俊平; 高橋是太郎; 永田光博; 伴 真俊; 矢部 衞; 山羽悦郎; 吉水 守; 阿部 周一編著
    北海道大学出版会, 25 Jan. 2010, 4832981935, 270, [Joint work]
  • 微生物の利用と制御 : 食の安全から環境保全まで
    藤井 建夫; 杉田 治男; 左子 芳彦, 微生物による魚病原因ウイルスの制御
    恒星社厚生閣, 2007, 9784769910671, Japanese, [Contributor]
■ Syllabus
  • 海洋生物工学特論Ⅱ, 2024年, 修士課程, 水産科学院
  • Introduction to Fisheries Sciences Ⅰ（水産科学汎論Ⅰ）, 2024年, 修士課程, 水産科学院
  • 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
  • 環境と人間, 2024年, 学士課程, 全学教育
  • 水産増殖学, 2024年, 学士課程, 水産学部
  • 微生物学実験, 2024年, 学士課程, 水産学部
  • 微生物学, 2024年, 学士課程, 水産学部
  • 魚病学, 2024年, 学士課程, 水産学部
  • 環境と人間, 2024年, 学士課程, 全学教育
■ Affiliated academic society
  • THE JAPANESE SOCIETY FOR MARINE BIOTECHNOLOGY
  • JAPAN AQUACULTURE SOCIETY
  • THE JAPANESE SOCIETY FOR VIROLOGY
  • THE JAPANESE SOCIETY OF FISHERIES SCIENCE
  • THE JAPANESE SOCIETY OF FISH PATHOLOGY
■ Research Themes
  • Establishment of a foundation for new ectoparasite control technology applicable to salmon broodstock for release and aquaculture.
    Grants-in-Aid for Scientific Research
    01 Apr. 2023 - 31 Mar. 2026
    笠井 久会; 工藤 秀明
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 23K05381
  • Pathogenic mechanism and control of soft egg disease in salmonids
    Grants-in-Aid for Scientific Research
    Apr. 2016 - Mar. 2020
    Kasai Hisae
    Soft egg disease in salmonids causes loss of firmness of the egg membrane, increasing vulnerability of the embryo to external impact. In this study, we show that the disease is caused when bacteria from the environment gradually dissolve the surface of the egg membrane. We analyzed bacteria attached to egg membranes, and found candidate pathogenic bacteria to be short bacilli. Artificial culture of the pathogen was possible and a specific detection method was subsequently developed. Furthermore, we demonstrate how polyphenols can inhibit egg membrane softening.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 16H04964
  • ヒラメレオウイルス感染症の防除に関する研究
    研究助成事業
    Apr. 2018 - Mar. 2019
    笠井久会
    公益財団法人北水協会, Principal investigator, Competitive research funding
  • サクラマスのヘルペスウイルス病および伝染性造血器壊死症防除 対策の強化に関する研究
    試験研究費補助金
    Apr. 2015 - Mar. 2016
    笠井久会
    公益財団法人北水協会, Principal investigator, Competitive research funding
  • マツカワのウイルス性神経壊死症防除対策の強化に関する研究
    試験研究費補助金
    Apr. 2012 - Mar. 2015
    笠井久会
    公益財団法人北水協会, Principal investigator, Competitive research funding
  • Studies of oncogenesis and viral pathogenesis of Oncorhunchus masou virus (OMV; Salmonid herpesvirus 2) in salmonid fish
    Grants-in-Aid for Scientific Research
    2013 - 2015
    Kasai Hisae
    Oncorhynchus masou virus (OMV; Salmonid herpesvirus 2) is a member of Herpisviridae associated with oncogenic and hepatitic diseases of national and international socio-economic importance. The present study focused on oncogenesis and viral pathogenesis of OMV in salmonid fish. Viral genes were localized in the whole Tumor tissues of masu salmon Oncorhynchus masou that survived OMV infections. A strong ISH signal was detected in the tumor tissue indicating that OMV induces tumor development. Highly expressed viral genes in Tumor were found by transcriptome analysis using NGS-based RNA sequencing. Liver failure as well as renal dysfunction and failure of gill function were occurred in OMV-infected rainbow trout O. mykiss.
    Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Competitive research funding, 25850132
  • カキ腸内由来細菌の産生する抗ウイルス物質の構造と活用技術の開発
    研究開発助成事業（talent補助金）
    Aug. 2012 - Feb. 2013
    笠井久会
    公益財団法人ノーステック財団, Principal investigator, Competitive research funding
  • Practical use of RNA vaccine at aquaculture field.
    Grants-in-Aid for Scientific Research
    2009 - 2012
    YOSHIMIZU Mamoru; KASAI Hisae; NISHIZAWA Toyohiko; TAKAMI Ikuo; OSEKO Norihisa; OH Myung-Joo
    In this study, we developed a novel fish-immunization with a synthetic double-stranded RNA [Poly(I:C)] and live pathogenic viruses. The fishes immunized with live pathogenic virus, followed by administration of Poly(I:C), became a transient, non-specific antiviral state, and those fish survive the initial immunization with live virus, thereby mounting a specific protective immune response against the injected pathogenic virus. Strong protective immune response was observed in rainbow trout, Japanese flounder and sevenband grouper by Poly(I:C)-immunization with IHNV, VHSV and NNV, respectively. It is conceivable that Poly(I:C)-immunization will eventually be applicable to a wide range of fish species as well as other viruses.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 21380112
  • 海洋環境中からの抗ウイルス物質産生細菌の探索および水産分野における活用技術の開発
    産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) 探索タイプ
    Aug. 2011 - 2011
    笠井 久会
    海洋環境中からの抗ウイルス物質産生細菌の効率的なスクリーニング方法を確立し、カキ消化管より分離した細菌が高い抗カリシウイルス活性を有することを明らかにした。次いで、本菌が産生する抗カリシウイルス活性物質の精製法を確立し、物質の情報を明らかにした。水産分野における活用技術として、カキ体内に蓄積されるノロウイルス(カリシウイルスの一種)対策に用いるため、本菌のカキへの投与法を開発した。以上は培養可能なカリシウイルスを用いたモデル実験であり、実際のカキ体内での有効性、ノロウイルスに対する抗ウイルス活性を検討していく必要があり、企業と早い段階で連携し、実用化に向けた研究を進める必要がある。
    国立研究開発法人科学技術振興機構, 北海道大学, Principal investigator, Competitive research funding
  • Development of vaccine against Oncorhynchus masou virus(OMV) disease based on whole genome analysis of OMV
    Grants-in-Aid for Scientific Research
    2010 - 2011
    KASAI Hisae
    Oncorhynchus masouvirus(OMV) disease is a hepatitic, oncogenic and skin ulcerative condition of national and international socio-economic importance. The OMV genome sequences of three strains including the high virulent strain against rainbow trout revealed about 160 kbp genome composed of a unique long sequence, and a unique short fragment flanked by inverted repeats. ORFs involved in pathogenicity or oncogenicity were found in OMV genome. Furthermore an effective vaccine was developed in which relative percent survival(RPS) reached 74%.
    Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 22780168
  • Produce a safe and worry free oyster for whole season
    Grants-in-Aid for Scientific Research(基盤研究(B))
    2005 - 2008
    Mamoru YOSHIMIZU; 田島 研一; Toyohiko NISHIZAWA; Tomoo SAWABE; Hisae KASAI; Kenichi TAJIMA
    20℃以上に調温した0.3mg/Lの塩素を含む電解海水あるいは中圧紫外線処理海水を用い、V. alginolyticus優勢キートセロスを給餌しながら浄化すれば、カキが痩せることなく、大腸菌、ノロウイルスおよびV. parahaemolyticusを排除できると考えられる。さらに安全性を確保するために2,000気圧の高静水圧をかけて脱殻を行えば、安全性はより向上する。
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 17380110
  • 環境調和型海水殺菌技法の開発と魚介類の飼育および食品衛生への応用に関する研究
    科学研究費助成事業
    2002 - 2004
    笠井 久会
    本年度,まず海水殺菌装置を使用する飼育施設ならびに対象とする病原体ごとのマニュアルを作成した。また,貝類の衛生管理に用いる殺菌装置の比較検討を行い,最適殺菌装置の選択を行い,さらに漁獲から加工場に至る間の水産物の衛生管理に向けた使用海水の最適殺菌条件を検討し,実用化に向けたマニュアルを作成した.その成果は以下の通りである。
    1.昨年度の成果より,海産種苗生産現場に甚大な被害を及ぼすウイルス性神経壊死症の原因ウイルスNNVに対しては海水電解殺菌装置が適していた。同装置で処理した海水でヒラメを飼育しても問題がなく,飼育用水の殺菌にはNNVが不活化される有効塩素濃度2.4mg/L以上の濃度での運転を推奨した。飼育排水の殺菌には,流量の問題から紫外線やオゾンを用いた殺菌装置では対応できず,本装置のみが対応可能であった。
    2.カキの浄化に紫外線殺菌海水や次亜塩素添加海水が用いられているが,低コストで大量の海水を処理することが出来る海水電解殺菌装置が最も適していた。北海道,宮城県および広島県で大腸菌の浄化試験を行い,紫外線と同等の除菌効果が得られた。また,培養不能なノロウイルスの代替ウイルスとしてFCVを供試し,電解処理したところ,有効塩素濃度0.43mg/Lで十分な不活化効果が得られた。同じ科に属すノロウイルスも同等の感受性を示すとすれば,感染性のノロウイルスを含まない浄化用水は,海水電解殺菌装置により十分得られることが明らかとなった。
    3.港内海水中の大腸菌および大腸菌群による漁獲物の汚染を防ぐには,海水電解殺菌装置が有効であったため,漁船8隻の取水管に本装置を取り付けた。環境へ悪影響を与えないため,十分な殺菌効果が得られる最低濃度である0.3mg/Lでの運転を推奨した。本装置により得られる殺菌海水の利用と,温度管理や他の衛生管理対策との相乗効果で,漁獲物の衛生管理の向上につながると考える。
    日本学術振興会, 特別研究員奨励費, 北海道大学, 02J00542
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  • 科学技術専門調査員
    2012 - 2017
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    文部科学省科学技術・学術政策研究所 科学技術予測センター
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    Aug. 2006 - 31 Mar. 2009
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