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Hashimoto Mamoru

Faculty of Information Science and Technology　Bioengineering and Bioinformatics　BioengineeringProfessor

Researcher basic information

■ Degree
  • Ph. D, Univ. of Tokyo, Jun. 1997
■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research Keyword
  • 非線形光学
  • 分光学
  • 応用光学
  • Spectroscopy
  • Applied Optics
Research Field
  • Nanotechnology/Materials, Optical engineering and photon science
  • Nanotechnology/Materials, Fundamental physical chemistry
  • Life Science, Biomaterials
  • Life Science, Biomedical engineering
■ Educational Organization

Career

■ Career
Career
  • Apr. 2019 - Present
    Hokkaido University, Division of Bioengineering and Bioinformatics, Faculty of Information Science and Engineering, professor
  • Oct. 2016 - Mar. 2019
    Hokkaido University, Graduate School of Information Science and Technology Division of Bioengineering and Bioinformatics, Professor
  • Apr. 2007 - Sep. 2016
    Osaka University, Graduate School of Engineering Science Department of Mechanical Science and Bioengineering Division of Bioengineering, Assoc. Professor
  • Apr. 2003 - Mar. 2007
    Osaka University, Graduate School of Engineering Science Department of Mechanical Science and Bioengineering Division of Bioengineering, 助教授
  • Nov. 2002 - Mar. 2003
    Osaka University, Graduate School of Engineering Science, Assoc. Professor
  • Apr. 1999 - Oct. 2002
    Osaka University, Graduate School of Engineering Science Department of Systems Innovation Division of Mathematical Science, 講師
  • Aug. 1997 - Mar. 1999
    Osaka University, Graduate School of Engineering Science, 助手
  • Apr. 1996 - Jul. 1997
    The University of Tokushima, Faculty of Engineering
  • Apr. 1991 - Mar. 1996
    神奈川科学技術アカデミー, 濵口「極限分子計測」プロジェクト, 研究員
Educational Background
  • Jun. 1997, The University of Tokyo, 大学院総合文化研究科 論文博士
  • Apr. 1989 - Mar. 1991, Osaka University, Graduate School of Engineering, Division of Applied Physics, Japan
  • Apr. 1985 - Mar. 1989, Osaka University, School of Engineering, 応用物理学科, Japan
Committee Memberships
  • Jan. 2020 - Present
    レーザ顕微鏡研究会 監査
  • 1998 - Present
    レーザ顕微鏡研究会, 幹事, Society
  • Apr. 2019 - Mar. 2021
    日本分光学会 北海道支部, 支部長, Society
  • Jan. 2019 - Dec. 2020
    日本光学会北海道支部, 幹事, Society
  • 2005 - Sep. 2016
    日本分光学会 関西支部, 幹事, Society
  • Jan. 2007 - Dec. 2008
    日本光学会, 光科学及び光技術調査委員会 委員長, Society

Research activity information

■ Awards
  • Jul. 2014, 国立大学法人 大阪大学, 第3回 大阪大学総長顕彰 研究部門
    橋本守
  • Feb. 2010, 財団法人 中谷医工計測技術財団, 中谷賞
    コヒーレントアンチスト―クスラマン散乱による生体分子の無染色な高解像・高速観測
    橋本 守
  • Aug. 2009, 高速電子処理応用技術学会, 優秀発表賞
    DSPを用いたピコ秒レーザーの高精度制御システムの開発
    橋本 守
  • Mar. 2009, 社団法人 日本機械学会, 2008年度日本機械学会船井賞
    テラヘルツ・カラースキャナーの開発
    安井武史;橋本守;荒木勉
  • Jan. 2006, 財団法人 コニカミノルタ画像科学振興財団, コニカミノルタ画像科学奨励賞
    分子のベクトル場的分布画像を観測する光学顕微鏡システム
    橋本 守, Japan
  • May 2002, 社団法人 日本分光学会, 日本分光学会賞論文賞
    コヒーレントアンチスト―クスラマン散乱を用いた顕微鏡
    橋本 守, Japan
■ Papers
■ Other Activities and Achievements
■ Books and other publications
  • CARS Microscopy: Implementation of nonlinear vibrational spectroscopy for far-field and near-field imaging
    Mamoru Hashimoto; Taro Ichimura; Katsumasa Fujita
    2012, English, [Peer-reviewed]
  • 顕微分光法 ナノ・マイクロの世界を見る分光法
    赤外・ラマン顕微分光法
    講談社サイエンティフィク, 2009, Japanese, Scholarly book, [Contributor]
  • バイオイメージングがわかる
    橋本守; 福島修一郎; 荒木勉, LIM蛍光寿命イメージング
    2005, Japanese, Scholarly book, [Contributor]
  • Biological Imaging and Sensing
    S. Kawata; O. Nakamura; T. Kaneko; M. Hashimoto; K. Goto; N. I. Smith; T. Sugiura; I. Fujimasa; H. Matsumoto, Biological imaging and sensing from basic techniques to clinical application
    Springer, 2004, 1-68, English, Scholarly book, [Contributor]
  • Handbook of Vibrational Spectroscopy
    Mamoru Hashimoto; Teturo Yuzawa; Chihiro Kato; Koichi Iwata; Hiro‐o Hamaguchi, Fast time-resolved mid-infrared spectroscopy using grating spectrometers
    John Wiley & Sons, Ltd, 2001, 666-676, English, Scholarly book, [Contributor]
■ Lectures, oral presentations, etc.
  • CMOSイメージセンサーを用いたマルチプレックス誘導ラマン散乱分光法”, "新しいイメージングを実現する最先端CMOSイメージセンサ
    De Xing Lioe; Shukri Bin Korakkottil; Kunhi Mohd; 本間 宗一郎; 大和 尚記; 安富 啓太; 香川 景一郎; 橋本 守; 川人 祥二
    Optics & Photonics Japan 2023, 27 Nov. 2023, Nominated symposium
    27 Nov. 2023 - 29 Nov. 2023
  • Development of Lissajous scanning fluorescence endomicroscope using polarization-maintaining fiber
    N. Yamato; M. Hashimoto
    OSJ-JSAP Joint Symposia on Optics, Optics & Photonics Japan 2023, 27 Nov. 2023, Oral presentation
    27 Nov. 2023 - 29 Nov. 2023
  • Imaging of trans lipid-mobilization by coherent Raman microspectroscopy
    S. Homma; N. Yamato; M. Hashimoto
    OSJ-JSAP Joint Symposia on Optics, Optics & Photonics Japan 2023, 27 Nov. 2023, Oral presentation
    27 Nov. 2023 - 29 Nov. 2023
  • Label-free imaging by coherent Raman scattering endoscopy
    M. Hashimoto
    2023 International Workshop on the New Frontiers in Convergence Science and Technology of Hokkaido University - Seoul National Universty 26th Joint Symposium, 02 Nov. 2023, English, Invited oral presentation
    02 Nov. 2023 - 02 Nov. 2023, [Invited]
  • 第二高調波発生関節鏡の開発とビーム走査手法の検討
    橋本守
    第21回医用分光学研究会, 30 Oct. 2023, Japanese, Invited oral presentation
    30 Oct. 2023 - 31 Oct. 2023, [Invited]
  • 光ファイバーレーザーを用いた無染色コラーゲン可視化間接鏡の開発
    橋本 守; 大和 尚紀; 松田 陸
    第62回日本生体医工学会北海道支部大会, 21 Oct. 2023
    21 Oct. 2023 - 21 Oct. 2023
  • Multiplex coherent anti-Stokes Raman 散乱分光顕微鏡を用いた脂肪様細胞のトランス脂肪動員観察
    本間 宗一郎; 大和 尚記; 橋本 守
    第84回応用物理学会秋季学術講演会, 20 Sep. 2023, Oral presentation
    19 Sep. 2023 - 23 Sep. 2023
  • Lipid metabolism imaging by elliptical spots scanning multiplex coherent anti-stokes raman scattering spectromicroscopy
    M. Hashimoto
    8th Asian Spectroscopy Conference 2023, 05 Sep. 2023, Invited oral presentation
    03 Sep. 2023 - 07 Sep. 2023, [Invited]
  • Examination of polarization compensation method using two electro-optic modulators
    R. Ikazaki; Y. Kawasaki; N. Yamato; M. Hashimoto
    Abstracts of the 58th Hokkaido Branch of the Japan Society of Applied Physics / the 19th Hokkaido Branch of the Optical Society of Japan, 07 Jan. 2023, Oral presentation
    07 Jan. 2023 - 08 Jan. 2023
  • 楕円スポット走査マルチプレックスCARS 顕微分光イメージング
    橋本守
    第19 回医用分光学研究会, 26 Nov. 2021, Invited oral presentation
    25 Nov. 2021 - 26 Nov. 2021, [Invited]
  • Ybファイバーレーザーを用いた第二高調波発生関節鏡の開発
    松田陸; 橋本守
    Optics & Photonics Japan 2021, 26 Oct. 2021, Oral presentation
    26 Oct. 2021 - 29 Oct. 2021
  • 楕円スポット型コヒーレント反ストークスラマン散乱分光顕微鏡のCARS光強度増強効果の実験的検証
    本間宗一郎; 橋本守
    第82回応用物理学会秋季学術講演会, 13 Sep. 2021, Oral presentation
    10 Sep. 2021 - 13 Sep. 2021
  • Real-time nerve extraction using coherent raman scattering rigid endoscope and deep learning
    N. Yamato; M. Matsuya; H. Niioka; J. Miyake; M. Hashimoto
    The 60 th Annual Conference of Japan Society for Medical and Biological Engineering, and The Anuual Meeting of Japan Biomagnetism and Bioelectromagnetics Society, 17 Jun. 2021, Japanese
    15 Jun. 2021 - 17 Jun. 2021
  • Evaluation of controlled release from doxorubicin-loaded nanoparticles under ultrasound irradiation
    M. Shinzato; Y. Kato; M. Hashimoto; N. Kudo
    The 60 th Annual Conference of Japan Society for Medical and Biological Engineering, and The Anuual Meeting of Japan Biomagnetism and Bioelectromagnetics Society, 15 Jun. 2021, Japanese, Oral presentation
    15 Jun. 2021 - 17 Jun. 2021
  • CARS硬性内視鏡の神経イメージングと深層学習による神経抽出ー実時間イメージングに向けた短時間露光神経画像の画像解析ー
    大和尚記; 松谷真奈; 新岡宏彦; 三宅淳; 橋本守
    第68回応用物理学会春季学術講演会, 16 Mar. 2021, Oral presentation
    19 Mar. 2021 - 19 Mar. 2021
  • 非線形ラマン散乱を用いた顕微内視鏡の開発
    橋本守
    4大学医工連携オンラインセミナー ～光×超音波×近赤外蛍光による医工連携イメージングと健康長寿への道～（onLine）, 19 Feb. 2021, Japanese, Invited oral presentation
    19 Feb. 2021 - 19 Feb. 2021, [Invited]
  • 楕円スポットを用いたコヒーレント反ストークスラマン散乱分光顕微鏡による信号増強効果の実験的検証
    本間 宗一郎; 阿部 隆爾; 橋本 守
    第 56 回 応用物理学会北海道支部／第 17 回 日本光学会北海道支部 合同学術講演会, 09 Jan. 2021, Oral presentation
    09 Jan. 2021 - 10 Jan. 2021
  • 光ファイバーバンドルを用いた非線形ラマン散乱顕微内視鏡
    橋本守; 小川拓希
    第18回医用分光学研究会, 27 Nov. 2020, Invited oral presentation
    26 Nov. 2020 - 27 Nov. 2020, [Invited]
  • 非線形ラマン散乱内視鏡に向けた光ファイバーバンドル内での四光波混合発生の低減法
    小川拓希; 橋本守
    Optics & Photonics Japan 2020, 14 Nov. 2020, Oral presentation
    14 Nov. 2020 - 17 Nov. 2020
  • 光ファイバーバンドルを用いた非線形ラマン散乱内視鏡の開発
    橋本守
    北海道大学 新技術説明会, 09 Nov. 2020, Invited oral presentation
    09 Nov. 2020 - 09 Nov. 2020, [Invited]
  • 神経を可視化するコヒーレントアンチストークスラマン散乱硬性内視鏡の可搬化のためのファイバーレーザー光源の開発
    松田陸; 大和尚記; 橋本守
    第59回日本生体医工学会北海道支部大会, 24 Oct. 2020, Oral presentation
    24 Oct. 2020 - 24 Oct. 2020
  • 非線形ラマン散乱硬性内視鏡と深層学習による無染色な末梢神経イメージングの検討
    大和尚記; 松谷真奈; 新岡宏彦; 三宅淳; 橋本守
    日本蛍光ガイド手術研究会第3回学術集会, 16 Oct. 2020, Invited oral presentation
    16 Oct. 2020 - 17 Oct. 2020, [Invited]
  • 非線形ラマン散乱硬性内視鏡と深層学習による神経イメージング装置の開発
    大和尚記; 松谷真奈; 新岡宏彦; 三宅淳; 橋本守
    レーザー学会第547回研究会, 09 Oct. 2020, Oral presentation
    09 Oct. 2020 - 09 Oct. 2020
  • High-speed hyperspectral imaging of living cells with beam scanning and slit acquisition polarization coherent {anti-Stokes} {Raman} scattering microspectroscopy
    Ryuji Abe; Mamoru Hashimoto
    The 81st JSAP Autumn Meeting, 10 Sep. 2020, Oral presentation
    08 Sep. 2020 - 11 Sep. 2020
  • 非線形ラマンイメージングの高速化 –深層学習と楕円形スポット–
    橋本 守
    日本学術振興会 生体ひかりイメージング技術と応用第185委員会, 03 Sep. 2020, Invited oral presentation
    03 Sep. 2020 - 03 Sep. 2020, [Invited]
  • ノイズ除去のアンサンブル学習による非線形ラマン硬性内視鏡神経イメージングの高速化
    大和尚記; 新岡宏彦; 橋本守
    第59回日本生体医工学会大会, 27 May 2020, Oral presentation
    25 May 2020 - 27 May 2020
  • High-speed hyperspectral imaging of living cells with slit-scanning multiplex coherent {anti-Stokes} {Raman} scattering microspectroscopy using elliptical focal spot
    Ryuji Abe; Kyotaro Horio; Shun Kizawa; Mamoru Hashimoto
    The 67th JSAP spring Meeting. Meeting was canceld (Abstract was published)., 15 Mar. 2020, Oral presentation
    12 Mar. 2020 - 15 Mar. 2020
  • 非線形ラマン硬性鏡による神経イメージの人工知能解析
    橋本守
    レーザー学会学術講演会第40回年次大会, 22 Jan. 2020, Invited oral presentation
    20 Jan. 2020 - 22 Jan. 2020, [Invited]
  • 二波長の後方散乱光を用いた無侵襲静脈血中脂質濃度推定法の提案
    下川部一真; 橋本守; 加藤祐次
    レーザー学会学術講演会第40回年次大会, 21 Jan. 2020, Oral presentation
    20 Jan. 2020 - 22 Jan. 2020
  • Comparison of deep learning models to improve imaging speed of coherent {Raman} scattering rigid endoscopy
    Naoki Yamato; Niioka Hirohiko; Jun Miyake; Mamoru Hashimoto
    The 55th JSAP Hokkaido Branch The 16th OSJ Hokkaido Branch Joint Meeting, 12 Jan. 2020, Oral presentation
    11 Jan. 2020 - 12 Jan. 2020
  • 非線形ラマン散乱硬性内視鏡と深層学習による神経イメージング
    大和尚記; 松谷真奈; 工藤信樹; 新岡宏彦; 三宅淳; 橋本守
    第32回日本内視鏡外科学会総会, 07 Dec. 2019, Oral presentation
    05 Dec. 2019 - 07 Dec. 2019
  • Nerve imaging and segmentation used by coherent Raman endoscopy and deep learning
    M. Hashimoto; N. Yamato; M. Matsuya; H. Niioka; J. Miyake
    Biomedical Raman Imaging 2019, 26 Nov. 2019, Invited oral presentation
    24 Nov. 2019 - 26 Nov. 2019, [Invited]
  • スリット走査マルチプレックスCARS顕微鏡の開発
    橋本守; 木澤駿
    第17回医用分光学研究会, 21 Nov. 2019, Invited oral presentation
    21 Nov. 2019 - 22 Nov. 2019, [Invited]
  • Live-cell observation with slit-scanning multiplex {C},{A},{R},{S} microspectroscopy using an elliptical focal spot
    Shun Kizawa; Mamoru Hashimoto
    The 80th JSAP Autumn Meeting 2019, 21 Sep. 2019, Oral presentation
    18 Sep. 2019 - 21 Sep. 2019
  • 転移学習を用いた深層学習による非線形ラマン像からの神経セグメンテーション
    松谷真奈; 大和尚記; 新岡彦; 工藤信樹; 三宅淳; 橋本守
    第80回応用物理学会秋季学術講演会, 21 Sep. 2019, Oral presentation
    18 Sep. 2019 - 21 Sep. 2019
  • コヒーレント反ストークスラマン散乱硬性鏡の開発と神経検出への応用
    橋本守
    第3期 第5回レーザー学会「レーザーバイオ医療」技術専門委員会, 26 Jul. 2019, Invited oral presentation
    [Invited]
  • Deep Learningと転移学習を用いたCARS硬性内視鏡イメージングの高速化
    新岡 宏彦; 大和 尚記; 三宅 淳; 橋本 守
    レーザ顕微鏡研究会第44回講演会・シンポジウム, 05 Jul. 2019, Oral presentation
    04 Jul. 2019 - 05 Jul. 2019
  • 非線形ラマン散乱硬性鏡による神経イメージングの転移学習を用いた高速化
    大和尚記; 新岡宏彦; 橋本守
    第58回日本生体医工学会大会, 08 Jun. 2019, Oral presentation
    06 Jun. 2019 - 08 Jun. 2019
  • Nerve segmentation from fluorescence images by using transferred deep learning
    Mana Matsuya; Naoki Yamato; Hirohiko Niioka; Mamoru Hashimoto
    Japanese Society of Molecular Imaging 2019, 24 May 2019, Oral presentation
    23 May 2019 - 24 May 2019
  • 楕円スポットを用いたスリット走査型マルチプレックスコヒーレントアンチストークスラマン散乱分光顕微鏡の開発
    木澤駿; 橋本守
    第66回応用物理学会春季学術講演会, 09 Mar. 2019, Oral presentation
    09 Mar. 2019 - 12 Mar. 2019
  • CARS 硬性鏡による神経イメージング
    橋本守
    第39回レーザー学会学術講演会 年次大会, 13 Jan. 2019, Invited oral presentation
    12 Jan. 2019 - 14 Jan. 2019, [Invited]
  • 非線形ラマン散乱硬性鏡を用いた神経イメージング
    橋本守
    第57回日本生体医工学会大会, 21 Jun. 2018, Invited oral presentation
    18 Jun. 2018 - 21 Jun. 2018, [Invited]
  • 非線形ラマンを用いたラベルフリージング
    橋本守
    一般社団法人レーザー学会学術講演会第３８回年次大会, 24 Jan. 2018, Invited oral presentation
    24 Jan. 2018 - 26 Jan. 2018, [Invited]
  • Development of coherent anti-Stokes Raman scattering rigid endoscope for label-free nerve imaging during an operation
    Hirose Keigo; Aoki Takuya; Fukushima Shuichiro; Sakaguchi Yoshiyuki; Furukawa Taichi; Hashimoto Mamoru
    Transactions of Japanese Society for Medical and Biological Engineering, 2016, Japanese Society for Medical and Biological Engineering, Japanese
    2016 - 2016,

    We have developed a coherent anti-Stokes Raman scattering (CARS) rigid endoscope for label-free nerve imaging during an operation. The CARS rigid endoscope consists of a single-mode fiber which delivers two color lasers, a galvano scanning system, and homemade ϕ12 mm lens tubes which include two relay lenses and an objective lens. The label-free imaging of rat sciatic nerve is demonstrated. To attain CARS image with high- speed, correcting chromatic aberration is focused on. The split between the focal spots of two lasers passed through the lens tubes is 21 μm and a numerical simulation shows that the intensity of CARS signal by correcting the chromatic aberration is 2.3 times as strong as non-correcting. The rigid endoscope has been modified to suppress the chromatic aberration by using two single-mode fibers which deliver the two lasers individually. The experimental result shows the increase of CARS intensity at least 1.5 times.

  • Label free bioimaging using nonlinear coherent Raman microscopy
    Mamoru Hashimoto
    IEEE CPMT Symposium Japan 2015: Packaging is Everywhere, ICSJ 2015, 14 Dec. 2015, Institute of Electrical and Electronics Engineers Inc., English
    14 Dec. 2015 - 14 Dec. 2015
  • Nonlinear Raman microscopy for biomedical applications(Workshop on microscopy, biology, medicine, and advanced CMOS imagers)
    Hashimoto Mamoru; Kawahito Shoji
    ITE Technical Report, 13 Nov. 2015, The Institute of Image Information and Television Engineers, English
    13 Nov. 2015 - 13 Nov. 2015, Nonlinear Raman imaging enables high-speed visualization of the biomolecules without staining. We have developed a multimodal microscopy and an endoscopy systems observing nonlinear Raman scattering. The developed systems are applied to the imaging of atherosclerosis legion. The performances of label-free imaging are demonstrated. Lock-in image sensor for parallel excitation/detection of stimulated Raman imaging is also demonstrated.
  • Label free bioimaging using nonlinear coherent Raman microscopy
    M. Hashimoto
    2015 IEEE CPMT Symposium Japan (ICSJ), Nov. 2015, English, Invited oral presentation
    [Invited]
  • Nonlinear Raman microscopy for biomedical applications
    M. Hashimoto; S. Kawahito
    Nov. 2015, English, Oral presentation
    [Invited]
  • PS2-3 Label free imaging of atherosclerotic lesions using stimulated Raman scattering, second harmonic generation, and two-photon fluorescence microscopy(PS2: Poster Short Presentation II,Poster Session)
    Aoki Takuya; Tao Tomoyo; Fukushima Syuichiro; Araki Tsutomu; Hashimoto Mamoru
    Proceedings of the ... Asian Pacific Conference on Biomechanics : emerging science and technology in biomechanics, 16 Sep. 2015, The Japan Society of Mechanical Engineers, English
    16 Sep. 2015 - 16 Sep. 2015
  • PS2-14 OBSERVATION OF CELLULLAR RESPONSE TO OXYGEN TENSION USING MICROFLUIDIC DEVICES(PS2: Poster Short Presentation II,Poster Session)
    Fujitaka Naoya; Fukushima Shuichiro; Hashimoto Mamoru; Funamoto Kenichi; Araki Tsutomu
    Proceedings of the ... Asian Pacific Conference on Biomechanics : emerging science and technology in biomechanics, 16 Sep. 2015, The Japan Society of Mechanical Engineers, English
    16 Sep. 2015 - 16 Sep. 2015
  • Plasmonic-nanoparticle-enhanced hyper-Raman spectroscopy
    B. Ranjan; M. Hashimoto; Y. Saito; P. Verma
    Sep. 2015, English, Oral presentation
  • Label free imaging of atherosclerotic lesions using stimulate Raman scattering, second harmonic generation, and two-photon fluorescence microscopy
    T. Aoki; T. Tao; S. Fukushima; T. Araki; M. Hashimoto
    Abstract of the 8th Asian-Pacific Conference on Biomechanics, Sep. 2015, English, Oral presentation
  • Discrete fourier transform infrared spectroscopy using precisely periodic pulse
    Y.-D. Hsieh; S. Okubo; H. Inaba; M. Hashimoto; T. Yasui
    May 2015, English, Oral presentation
    [International presentation]
  • Imaging of atherosclerosis legion using multimodal nonlinear optical microscopy
    M. Hashimoto
    May 2015, English, Oral presentation
    [Invited]
  • Biological high-resolution imaging in wet-condition using cathodoluminescence microscopy
    T. Furukawa; S. Fukushima; H. Niioka; M. Ichimiya; J. Miyake; M. Ashida; T. Araki; M. Hashimoto
    Proceedings of The 5th Asian and Pacific-Rim Symposium on Biophotonics, May 2015, English, Oral presentation
  • 近赤外発光・カソードルミネッセンスによるマルチスケール生体観察
    福島昌一郎; 新岡宏彦; 一宮正義; 三宅 淳; 芦田昌明; 荒木 勉; 橋本 守
    Mar. 2015, Japanese
    Mar. 2015 - Mar. 2015
  • Discrete Fourier Transform Infrared Spectroscopy Using Precisely Periodic Pulse
    Y. -D. Hiseh; S. Okubo; H. Inaba; M. Hashimoto; T. Yasui
    2015 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO), 2015, English
    2015 - 2015
  • Highly luminescent rare-earth doped Y2O3 ceramics in near-infrared region by codoping Li+ ions and its application for imaging
    J. Yamasaki; S. Fukushima; H. Niioka; T. Araki; M. Hashimoto; J. Miyake
    Dec. 2014, English
  • Gadolinium oxide doped rare earth nanophosphors for trimodal imaging
    D. T; K. Dung; S. Fukushima; H. Niioka; M. Ichimiya; M. Ashida; T. Araki; M. Hashimoto; J. Miyake
    Dec. 2014, English
  • Observation of organic nonlinear optical crystal by multiplex fourth order Raman microscope
    C. Ninagawa; T. furukawa; H. Niioka; T. Araki; M. Hashimoto
    Dec. 2014, English
  • A low-noise stimulated Raman scattering CMOS imager using high-speed lock-in pixels(Poster session,2nd Asian Image Sensors and Imaging Systems Symposium)
    Lioe De Xing; Mars Kamel; Han Sang Man; Takasawa Taishi; Yasutomi Keita; Kagawa Keiichiro; Hashimoto Mamoru; Kawahito Shoji
    ITE Technical Report, 24 Nov. 2014, The Institute of Image Information and Television Engineers, English
    24 Nov. 2014 - 24 Nov. 2014, We demonstrate a low-noise stimulated Raman scattering (SRS) CMOS imager using high-speed lock-in pixels. To detect small SRS signal under an extremely large offset due to probing laser signal of which the ratio is 10^<-5>, a lock-in pixel using a high-speed demodulator, low-pass filter, and multiple-sampling amplifier is designed. The residual offset and low-frequency noise are reduced by two steps; one is sampling phase adjusting in the multiple-sampling amplifier and the other is a double modulation technique. The double modulation effectively reduces the offset and low-frequency noise, or 1/f noise. A SRS CMOS imager chip for the proof of concept is implemented and a successful operation is demonstrated. The detectable AC signal level under the large DC offset is measured to be smaller than ratio of 10^<-4>. This finding suggests that the detection of a very small SRS signal from a biological sample is feasible using this technique.
  • 近赤外光・電子顕微鏡による相関バイオイメージング
    福島昌一郎; 新岡宏彦; 一宮正義; 三宅 淳; 芦田昌明; 荒木 勉; 橋本 守
    Oct. 2014, Japanese
    Oct. 2014 - Oct. 2014
  • Observation of arteriosclerotic lesion using coherent nonlinear Raman imaging
    Mamoru Hashimoto; Shoji Kawahito
    Transactions of Japanese Society for Medical and Biological Engineering, 17 Aug. 2014, Japan Soc. of Med. Electronics and Biol. Engineering, English
    17 Aug. 2014 - 17 Aug. 2014
  • Histological assessment of atherosclerosis by nonlinear optical multimodal microscopy
    Tomoyo Tao; Harsono Cahyadi; Shuichiro Fukushima; Mamoru Hashimoto; Tsutomu Araki
    Transactions of Japanese Society for Medical and Biological Engineering, 17 Aug. 2014, Japan Soc. of Med. Electronics and Biol. Engineering, English
    17 Aug. 2014 - 17 Aug. 2014
  • 近赤外・カソードルミネッセンス相関観察を目指したナノ蛍光体粒子の開発
    福島昌一郎; 新岡宏彦; 一宮正義; 三宅 淳; 芦田昌明; 荒木 勉; 橋本 守
    Jul. 2014, Japanese
    Jul. 2014 - Jul. 2014
  • Cathodoluminescence and Fluorescent Bioimaging with Using Rare-Earth Doped Nanophorspohrs
    Niioka Hirohiko; Furukawa Taichi; Mamoru Hashimoto
    顕微鏡, Apr. 2014, Japanese
    Apr. 2014 - Apr. 2014, [Invited]
  • カソードルミネッセンス顕微鏡と光学顕微鏡の融合
    新岡宏彦; 古川太一; 福島昌一郎; 一宮正義; 三宅 淳; 芦田昌明; 荒木 勉; 橋本 守
    日本顕微鏡学会バイオメディカルニュ－マイクロスコ－プ分科会 平成25年度シンポジウム講演会, 06 Mar. 2014, Japanese
    06 Mar. 2014 - 06 Mar. 2014, [Invited]
  • 希土類ナノ蛍光体を用いたマルチカラーカソードルミネッセンス・アップコンバージョン生体観察
    福島昌一郎; 古川太一; 新岡宏彦; 一宮正義; 芦田昌明; 三宅 淳; 荒木 勉; 橋本 守
    Mar. 2014, Japanese
    Mar. 2014 - Mar. 2014
  • Synthesis of nanophosphors for correlative cathodoluminescence, up-conversion, and near-infrared bioimaging
    S. Fukushima; T. Furukawa; H. Niioka; M. Ichimiya; J. Miyake; M. Ashida; T. Araki; M. Hashimoto
    Mar. 2014, English
    Mar. 2014 - Mar. 2014
  • Visible to near-infrared luminescent nanoparticles for multimodal bioimaging on nanometer to millimeter scale
    S. Fukushima; T. Furukawa; H. Niioka; M. Ichimiya; J. Miyake; M. Ashida; T. Araki; M. Hashimoto
    Japan Taiwan Bilateral Conference on Biomedical and Plasmonic Imaging, Feb. 2014, English
    Feb. 2014 - Feb. 2014
  • 1F16 Histological assessment of atherosclerosis by nonlinear optical multimodal microscopy
    TAO Tomoyo; FUKUSHIMA Shuichiro; HASHIMOTO Mamoru; ARAKI Tsutomu
    バイオエンジニアリング講演会講演論文集, 10 Jan. 2014, The Japan Society of Mechanical Engineers, Japanese
    10 Jan. 2014 - 10 Jan. 2014
  • Development of nonlinear optical multimodal microscopy system for histological assessment of atherosclerosis
    Tao Tomoyo; Harsono Cahyadi; Fukushima Shuichiro; Hashimoto Mamoru; Araki Tsutomu
    BME, 2014, Japanese Society for Medical and Biological Engineering, Japanese
    2014 - 2014, We developed a nonlinear optical multimodal microscopy system for diagnostic tool of atherosclerosis. Nonlinear optical microscopy is based on the nonlinear optcal phenomena originated from optical properties of materials. It avoids the need for invasive dyes. In particular, coherent Raman scattering (CRS) techniques provide label-free detection due to the intrinsic vibrational signatures of the specific molecules. Therefore it offers a non-invasive imaging of lipid in atherosclerotic plaques. The second harmonic generation (SHG) is exclusively sensitive to noncentrosymmetric materials. Hence, it enables a label-free detection of collagen which is known to be unpredictably formed around the plaques. Therefore, CRS/SHG multimodal microscopy system is a potential tool for quantitative diagnosis of atherosclerosis.
    In this study, we provided a histological assessment of abnormally produced collagen in aorta samples of mice, and our results indicated that the collagen had a correlation with the progression of lesion. Furthermore, we performed CRS/SHG imaging with the developed system.
  • B204 Correlative bioimaging with near-infrared and electron microscopy
    Shoichiro FUKUSHIMA; Hirohiko NIIOKA; Masayoshi ICHIMIYA; Masaaki ASHIDA; Jun MIYAKE; Tsutomu ARAKI; Mamoru HASHIMOTO
    The Proceedings of the JSME Conference on Frontiers in Bioengineering, 2014, Japan Society of Mechanical Engineers
    2014 - 2014
  • Lock-in Pixels Readout Circuit Using a High Speed Lateral Electric Field Modulator with Differential Charge Accumulation for Stimulated Raman Scattering Imager
    Kamel Mars; Ken Egawa; Lioe De Xing; Han Sang Man; Taishi Takasawa; Keita Yasutomi; Keiichiro Kagawa; Mamoru Hashimoto; Shoji Kawahito
    2014 IEEE 12TH INTERNATIONAL NEW CIRCUITS AND SYSTEMS CONFERENCE (NEWCAS), 2014, English
    2014 - 2014
  • 3P-247 Development of near infrared phosphor probes for deep tissue imaging toward the application for regenerative medicine
    Niioka Hirohiko; Fukushima Syoichiro; Hashimoto Mamoru; Araki Tsutomu; Onoshima Daisuke; Yukawa Hiroshi; Baba Yoshinobu; Miyake Jun
    日本生物工学会大会講演要旨集, 2014, 日本生物工学会, Japanese
    2014 - 2014
  • Tens nanometer scale cathodoluminescence bioimaging with rare-earth doped nanophosphors
    Shoichiro Fukushima; Hirohiko Niioka; Masayoshi Ichimiya; Masayoshi Ichimiya; Jun Miyake; Masaaki Ashida; Tsutomu Araki; Mamoru Hashimoto
    JSAP-OSA Joint Symposia, JSAP 2014, Jan. 2014
    Jan. 2014 - Jan. 2014
  • Observation of anhydrated and hydrated DAST crystals using multiplex fourth order Raman microscope
    Chikako Ninagawa; Hirohiko Niioka; Tsutomu Araki; Mamoru Hashimoto
    JSAP-OSA Joint Symposia, JSAP 2014, Jan. 2014
    Jan. 2014 - Jan. 2014
  • Multimodal bioimaging probes based on lanthanide doped Gd2O3 nanophosphors
    Doan T. Kim Dung; Shoichiro Fukushima; Hirohiko Niioka; Masayoshi Ichimiya; Masayoshi Ichimiya; Masaaki Ashida; Tsutomu Araki; Mamoru Hashimoto; Jun Miyake
    JSAP-OSA Joint Symposia, JSAP 2014, Jan. 2014
    Jan. 2014 - Jan. 2014
  • 電子線及び近赤外光照射による発光を用いたバイモーダル生体観察
    福島昌一郎; 古川太一; 新岡宏彦; 一宮正義; 芦田昌明; 三宅 淳; 荒木 勉; 橋本 守
    Nov. 2013, Japanese
    Nov. 2013 - Nov. 2013
  • マルチスケール生体イメージングを目指したカソードルミネッセンス・アップコンバージョンナノ蛍光体の作製
    福島昌一郎; 古川太一; 新岡宏彦; 一宮正義; 芦田昌明; 三宅 淳; 荒木 勉; 橋本 守
    Nov. 2013, Japanese
    Nov. 2013 - Nov. 2013
  • Upconversion fluorescence and CL imaging for multiscale biological imaging
    H. Niioka; T. Furukawa; S. Fukushima; M. Ichimiya; J. Miyake; M. Ashida; T. Araki; M. Hashimoto
    Proceedings of 2013 Annual Meeting of the Spectroscopical Society of Japan, Nov. 2013, English
    Nov. 2013 - Nov. 2013
  • Bimodal biological observation with luminescence emitted under electron beam and near-infrared light irradiation
    S. Fukushima; T. Furukawa; H. Niioka; M. Ichimiya; J. Miyake; M. Ashida; T. Araki; M. Hashimoto
    Proceedings of 2013 Annual Meeting of the Spectroscopical Society of Japan, Nov. 2013, English
    Nov. 2013 - Nov. 2013
  • A220 Observation of atherosclerosis by second harmonic generation microscopy
    TAO Tomoyo; FUKUSHIMA Shuichiro; HASHIMOTO Mamoru; ARAKI Tsutomu
    Proceedings of the ... JSME Conference on Frontiers in Bioengineering, 31 Oct. 2013, The Japan Society of Mechanical Engineers, Japanese
    31 Oct. 2013 - 31 Oct. 2013
  • マルチスケール相関生体イメージングを実現するバイモーダルナノ蛍光体の合成
    福島昌一郎; 古川太一; 新岡宏彦; 一宮正義; 三宅 淳; 芦田昌明; 荒木 勉; 橋本 守
    Oct. 2013, Japanese
    Oct. 2013 - Oct. 2013
  • Upconversion Nanophosphors for Correlative CL and Fluorescent Imaging
    H. Niioka; T. Furukawa; S. Fukushima; M. Ichimiya; T. Nagata; J. Miyake; M. Ashida; T. Araki; M. Hashimoto
    Oct. 2013, English
    Oct. 2013 - Oct. 2013
  • Towards multicolor correlative light and cathodoluminescence imaging with using upconversion nanophosphors
    S. Fukushima; T. Furukawa; H. Niioka; M. Ichimiya; M. Ichimiya; T. Nagata; J. Miyake; M. Ashida; T. Araki; M. Hashimoto
    JSAP-OSA Joint Symposia 2013, Sep. 2013
    Sep. 2013 - Sep. 2013
  • カソードルミネッセンス顕微鏡を用いたバイオイメージング
    古川太一; 新岡宏彦; 福島昌一郎; 一宮正義; 市川 聡; 永田智啓; 芦田昌明; 荒木 勉; 橋本 守
    Jul. 2013, Japanese
    Jul. 2013 - Jul. 2013
  • A High-Speed Modulation Lock-in Pixel Imager Using Differential Charge Accumulation for Stimulated Raman Scattering Spectroscopy
    MARS Kamel; BEAK Guseul; HANG Sang Man; TAKASAWA Taishi; YASUTOMI Keita; KAGAWA Keiichiro; HASHIMOTO Mamoru; KAWAHITO Shoji
    電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan, 26 Mar. 2013, English
    26 Mar. 2013 - 26 Mar. 2013
  • アップコンバージョン発光とカソードルミネッセンスによる生体観察を目指した希土類ナノ蛍光体プローブの作製
    福島昌一郎; 古川太一; 新岡宏彦; 一宮正義; 永田智啓; 三宅 淳; 芦田昌明; 荒木 勉; 橋本 守
    Mar. 2013, Japanese
    Mar. 2013 - Mar. 2013
  • カソードルミネッセンス生体イメージングのための微小希土類添加ナノ蛍光体作製
    古川太一; 新岡宏彦; 一宮正義; 市川 聡; 永田智啓; 三宅 淳; 芦田昌明; 荒木 勉; 橋本 守
    Mar. 2013, Japanese
    Mar. 2013 - Mar. 2013
  • Synthesis of Rare-earth Doped Nano Phosphors for Biological Cathodoluminescence Imaging
    T. Furukawa; H. Niioka; M. Ichimiya; S. Ichikawa; T. Nagata; J. Miyake; M. Ashida; T. Araki; M. Hashimoto
    Focus on Microscopy 2013 (Maastricht, The Netherlands) (Poster), Mar. 2013, English
    Mar. 2013 - Mar. 2013
  • C212 Synthesis of bimodal nanophosphors with cathodoluminescence and upconversion luminescence for multi-scale bioimaging
    Shoichiro FUKUSHIMA; Taichi FURUKAWA; Hirohiko NIIOKA; Mamoru Hashimoto
    The Proceedings of the JSME Conference on Frontiers in Bioengineering, 2013, Japan Society of Mechanical Engineers, Japanese
    2013 - 2013
  • Rare-earth Doped Y2O3 Nanophosphors Synthesized for Bio-imaging with Using CL and Fluorescence Microscopy
    H. Niioka; T. Furukawa; M. Ichimiya; T. Nagata; M. Ashida; T. Araki; M. Hashimoto
    8th Handai Nanoscience and Nanotechnology International Symposium, Dec. 2012, English
    Dec. 2012 - Dec. 2012
  • A Draining-Only Modulation CMOS Image Sensor for Fluorescence Lifetime Imaging
    YASUTOMI Keita; LI Zhuo; KAGAWA Keiichiro; NIIOKA Hirohiko; HASHIMOTO Mamoru; KAWAHITO Shoji
    ITE Technical Report, 14 Nov. 2012, The Institute of Image Information and Television Engineers, Japanese
    14 Nov. 2012 - 14 Nov. 2012, The paper presents a time-resolved CMOS image sensor with Draining Only Modulation(DOM) pixels for fluorescence lifetime imaging. The proposed DOM pixels, which enables signal charge transfer without any transfer gates, provides high-speed charge modulation and repetitive accumulation at very low light level. The prototype imager demonstrates loss-free charge accumulation and fluorescence lifetime measurements for different fluorescent samples.
  • 希土類添加Y2O3ナノ蛍光体を用いたマルチモーダル蛍光・CL細胞イメージング
    新岡宏彦; 古川太一; 一宮正義; 永田智啓; 芦田昌明; 荒木 勉; 橋本 守
    Nov. 2012, Japanese
    Nov. 2012 - Nov. 2012
  • OS3-1-3 Cellular imaging with using cathodoluminescence and rare earth doped nanophosphors
    Furukawa Taichi; Niioka Hirohiko; Ichimiya Masayoshi; Nagata Tomohiro; Ashida Masaaki; Araki Tsutomu; Hashimoto Mamoru
    マイクロ・ナノ工学シンポジウム, 21 Oct. 2012, The Japan Society of Mechanical Engineers, Japanese
    21 Oct. 2012 - 21 Oct. 2012, Bio molecular imaging is important to clarify cellular functions. Cathodoluminescence (CL) is light emission from the materials excited by accelerated electron beam, and CL microscopy has the potential to enable color imaging of individual biomolecular distributions with using immunolabeling at high spatial resolution. Because of electron beam excitation the spatial resolution reaches about 10 nm. In this study, we demonstrated CL imaging for cells using rare-earth doped nanophosphors at high spatial resolution.
  • 光学顕微鏡とカソードルミネッセンス顕微鏡を用いたマルチモーダル細胞イメージング
    新岡宏彦; 古川太一; 一宮正義; 永田智啓; 芦田昌明; 荒木勉; 橋本守
    Sep. 2012, Japanese
    Sep. 2012 - Sep. 2012
  • Multimodal Imaging via Light Microscopy and Cathodoluminescence Microscopy for Biological Specimens with Rare-earth Doped Nanophosphors
    T. Furukawa; H. Niioka; M. Ichimiya; T. Nagata; M. Ashida; T. Araki; M. Hashimoto
    JSAP-OSA joint Symposia 2012, the 73th Autumn Meeting, Sep. 2012, English
    Sep. 2012 - Sep. 2012
  • ナノ蛍光体粒子とカソードルミネッセンス顕微鏡を用いたマルチカラー生体イメージング
    新岡宏彦; 古川太一; 一宮正義; 芦田昌明; 荒木勉; 橋本守
    日本顕微鏡学会 (つくば国際会議場, 2012/5/14-16, 招待講演), May 2012, Japanese
    May 2012 - May 2012, [Invited]
  • Well-dispersed nanophosphors for cathodoluminescence microscopy produced by using laser ablation method
    T. Furukawa; H. Niioka; M. Ichimiya; T. Nagata; M. Ashida; T. Araki; M. Hashimoto
    Program and Abstract Book, Focus on Micrscopy 2012 (FOM2012), Apr. 2012, English
    Apr. 2012 - Apr. 2012
  • 希土類ナノ蛍光体を用いた生体カソードルミネッセンスイメージングの高輝度化
    古川太一; 新岡宏彦; 一宮正義; 永田智啓; 芦田昌明; 荒木 勉; 橋本 守
    Mar. 2012, Japanese
    Mar. 2012 - Mar. 2012
  • ナノ蛍光体粒子を用いたカソードルミネッセンス生体イメージング
    新岡宏彦; 古川太一; 一宮正義; 芦田昌明; 荒木 勉; 橋本 守
    Feb. 2012, Japanese
    Feb. 2012 - Feb. 2012
  • 7D32 Intracellular imaging of anticancer drug using CARS microscopy
    IKEDA Kouhei; CAHYADI Harsono; NIIOKA Hirohiko; ARAKI Tsutomu; HASHIMOTO Mamoru
    バイオエンジニアリング講演会講演論文集, 06 Jan. 2012, The Japan Society of Mechanical Engineers, English
    06 Jan. 2012 - 06 Jan. 2012
  • 7D45 Observation of biological molecules using hyper-Raman microspectroscopy
    KANOH Hiroto; NIIOKA Hirohiko; ARAKI Tsutomu; HASHIMOTO Mamoru
    バイオエンジニアリング講演会講演論文集, 06 Jan. 2012, The Japan Society of Mechanical Engineers, Japanese
    06 Jan. 2012 - 06 Jan. 2012
  • 7D44 Distinction between triglyceride deposit cardiomyovasculopathy and ischemic cardiomyopathy with Raman microscopy and multivariable analysis
    MATSUMURA Naokazu; NIIOKA Hirohiko; IKEDA Yoshihiko; HIRANO Ken-ichi; ARAKI Tsutomu; HASHIMOTO Mamoru
    バイオエンジニアリング講演会講演論文集, 06 Jan. 2012, The Japan Society of Mechanical Engineers, Japanese
    06 Jan. 2012 - 06 Jan. 2012
  • 7D31 Development of CARS microscopy using picosecond high speed wavelength scanning laser
    IWATSUKA Junichi; MINAMIKAWA Takeo; NIIOKA Hirohiko; ARAKI Tsutomu; HASHIMOTO Mamoru
    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME, 2012, The Japan Society of Mechanical Engineers, Japanese
    2012 - 2012
  • 7D46 Photodamage to cells in multi-focus nonlinear Raman microscopy
    MURAKAMI Yoshinori; MINAMIKAWA Takeo; ARAKI Tsutomu; HASHIMOTO Mamoru
    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME, 2012, The Japan Society of Mechanical Engineers, Japanese
    2012 - 2012
  • Super-resolution imaging of nano phosphors via cathodoluminescence microscopy for biological imaging
    H. Niioka; T. Furukawa; M. Ichimiya; M. Ashida; T. Araki; M. Hashimoto
    Dec. 2011, English
    Dec. 2011 - Dec. 2011
  • ナノ蛍光体粒子を用いたマルチカラー生体カソードルミネッセンスイメージング
    古川太一; 新岡宏彦; 一宮正義; 永田智啓; 芦田昌明; 荒木 勉; 橋本 守
    Nov. 2011, Japanese
    Nov. 2011 - Nov. 2011
  • Super-Resolution Imaging for Cells by using Cathodoluminescence
    T. Furukawa; H. Niioka; M. Ichimiya; M. Ashida; T. Araki; M. Hashimoto
    Sep. 2011, Japanese
    Sep. 2011 - Sep. 2011
  • カソードルミネッセンスを利用した生体細胞の超解像イメージング手法
    古川太一; 新岡宏彦; 一宮正義; 芦田昌明; 荒木 勉; 橋本 守
    Jul. 2011, Japanese
    Jul. 2011 - Jul. 2011
  • T0302-2-3 Noncontact and nondestructive measurement of strain using three-dimensional molecular orientation measurement under microscope
    YOSHIKI Keisuke; Masuda Kyosuke; HASHIMOTO Mamoru; HASHIMOTO Nobuyuki; KURIHARA Makoto; Namazu Takahiro; INOUE Shozo
    The proceedings of the JSME annual meeting, 2010, The Japan Society of Mechanical Engineers, Japanese
    2010 - 2010, We developed a system to analyze microscopic deformation, which can visualize inhomogeneous deformation in MEMS(Micro Electro Mechanical Systems) devices by using single-molecule markers. As MEMS devices become smaller, their mechanical properties seem to become inhomogeneous. This causes an undesirable distribution of deformation and stress, and decreases the reliability of the MEMS devices. We distributed fluorescent single molecules discretely on the microstructure of a MEMS device, and traced their relative displacements and rotations by observation under a three-dimensional orientation microscope, which provided information about normal, torsional, and bending displacements.
  • B201 Label-free and real-time CARS imaging of living cell reactions in laser-induced ablation
    MINAMIKAWA Takeo; NIIOKA Hirohiko; ARAKI Tsutomu; HASHIMOTO Mamoru
    The Proceedings of the JSME Conference on Frontiers in Bioengineering, 2009, The Japan Society of Mechanical Engineers, Japanese
    2009 - 2009
  • Realtime biomolecular imaging by multifocus CARS microscopy
    2009
  • High-speed non-staining biomolecular imaging by nonlinear Raman microspectroscopy
    2009
  • Non-staining Visualization of Collagen by Second Harmonic Generation Microscopy
    FUKUSHIMA Shuichiro; YASUI Takeshi; HASHIMOTO Mamoru; ARAKI Tsutomu
    可視化情報学会誌. Suppl., 01 Jul. 2008, Japanese
    01 Jul. 2008 - 01 Jul. 2008
  • 1203 Second harmonic generation imaging of organic molecule on metal by radial polarization excitation
    Ashida Koichiro; Yoshiki Keisuke; Hashimoto Mamoru; Araki Tsutomu
    関西支部講演会講演論文集, 14 Mar. 2008, The Japan Society of Mechanical Engineers, Japanese
    14 Mar. 2008 - 14 Mar. 2008
  • 602 Label-free, high-speed imaging with real-time CARS microscope
    MINAMIKAWA Takeo; HASHIMOTO Mamoru; ARAKI Tsutomu
    The Proceedings of Conference of Kansai Branch, 2008, The Japan Society of Mechanical Engineers, Japanese
    2008 - 2008
  • 329 Development of second-harmonic-generation microscope to determine 3-dimensional molecular orientation with 3-dimensional spatial resolution
    YOSHIKI Keisuke; HASHIMOTO Mamoru; ARAKI Tsutomu
    バイオエンジニアリング講演会講演論文集, 12 Jan. 2006, The Japan Society of Mechanical Engineers, Japanese
    12 Jan. 2006 - 12 Jan. 2006
  • Three dimensional polarization control and its application to SHG imaging
    K. Yoshiki; M. Hashimoto; T. Araki
    2005 Pacific Rim Conference on Lasers & Electro-Optics, Jul. 2005
    Jul. 2005 - Jul. 2005
  • Multi-focus CARS microscopy using automatic pulse duration control system
    Proceedings of International Conference on Quantum Electronics 2005 and the Pacific Rim Conference on Lasers and Electro-Optics 2005 (IQEC/CLEO-PR 2005), 2005
  • 金属プローブを用いた近接場CARSイメージング
    市村垂生; 早沢紀彦; 橋本守; 井上康志; 河田聡
    日本分光学会講演要旨集, 18 May 2004, Japanese
    18 May 2004 - 18 May 2004
  • 局所プラズモン増強効果を用いた高空間分解CARSイメージング
    市村垂生; 早沢紀彦; 橋本守; 井上康志; 河田聡
    応用物理学関係連合講演会講演予稿集, 28 Mar. 2004, Japanese
    28 Mar. 2004 - 28 Mar. 2004
  • チップ増強非線形ラマン散乱を用いた近接場振動分光
    市村垂生; 早沢紀彦; 橋本守; 井上康志; 河田聡
    応用物理学関係連合講演会講演予稿集, 28 Mar. 2004, Japanese
    28 Mar. 2004 - 28 Mar. 2004
  • Coherent anti-stokes Raman nano-imaging with metal-tip field enhancement
    N Hayazawa; T Ichimura; M Hashimoto; Y Inouye; S Kawata
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Mar. 2004, English
    Mar. 2004 - Mar. 2004
  • Coherent anti-Stokes Raman microscope for identification of cellular molecule
    Proceedings of the Second Asian and Pacific Rim Symposium on Biophotonics 234-235, 2004
  • Fluorescence lifetime properties of various calcium ion indicators
    Proc. ICO-14, 2004
  • 金ナノ微粒子によるコヒーレントアンチストークスラマン散乱の局所増強
    市村垂生; 早沢紀彦; 橋本守; 荒木勉; 井上康志; 河田聡
    レーザ顕微鏡研究会講演会論文集, 01 Jul. 2003, Japanese
    01 Jul. 2003 - 01 Jul. 2003
  • 金属ナノ微粒子による局所増強コヒーレントアンチストークスラマン散乱
    市村垂生; 早沢紀彦; 橋本守; 井上康志; 河田聡
    応用物理学関係連合講演会講演予稿集, 27 Mar. 2003, Japanese
    27 Mar. 2003 - 27 Mar. 2003
  • Coherent anti-Stokes Raman scattering microscopy using near IR exitation and UV excitation
    Ueda Kei; Hashimoto Mamoru; Araki Tsutomu; Kawata Satoshi
    バイオエンジニアリング講演会講演論文集, 20 Jan. 2003, The Japan Society of Mechanical Engineers, Japanese
    20 Jan. 2003 - 20 Jan. 2003
  • Confocal Fluorescence Lifetime Imaging by Asynchronous Sampling
    AZUMA Hiroki; INAMOTO Hirohisa; HASHIMOTO Mamoru; ARAKI Tsutomu; CHIKAMORI Kensuke
    関西支部講演会講演論文集, 2003, The Japan Society of Mechanical Engineers, Japanese
    2003 - 2003, We have developed a confocal microscope for fluorescence lifetime imaging by asynchronous sampling. This system consists of a fast response photomultiplicr, a fast sampling module, and a low-pass filter and a colnfocal microscope. Since imaging by laser scanning and detection is asynchronous with laser excitation. It is possible to attach the detection system to the commercial confocal microscopy system. We also observed the relationship between the concentration of Ca^<2+> and the fluorescence lifetime of fluo-3,and confirmed that the fluorescence lifetime is sensitive to the concentration of Ca^<2+>.
  • Coherent anti-Stokes Raman scattering microscopy
    HASHIMOTO Mamoru
    Electron-microscopy, 27 Nov. 2002, 日本電子顕微鏡学会, Japanese
    27 Nov. 2002 - 27 Nov. 2002
  • Coherent Anti-Stokes Raman Scattering Microscopy
    HASHIMOTO Mamoru; ARAKI Tsutomu; KAWATA Satoshi
    IEICE technical report. ME and bio cybernetics, 11 Oct. 2002, The Institute of Electronics, Information and Communication Engineers, Japanese
    11 Oct. 2002 - 11 Oct. 2002, New multiphoton microscopy using CARS (coherent anti-Stokes Raman scattering) spectroscopy is described. In CARS microscopy, the image of molecular vibration that is sensitive to the molecular spices and conformation is obtainable without staining with three-dimensional resolution of sub-micrometer. A picosecond tunable laser and suitable optical filters provide the CARS imaging in the fingerprint region, and multi-focus excitation using a rotatory-microlens array enables the multi-spectral imaging.
  • Resonance enhancement of coherent anti-Stokes Raman scattering microscopy
    Ueda Kei; Hashimoto Mamoru; Araki Tsutomu; Kawata Satoshi
    Proceedings of the JSME Bioengineering Conference and Seminar, 2002, The Japan Society of Mechanical Engineers, Japanese
    2002 - 2002
  • Capillary electrophoresis system using a fluorescence labeled cell as a sensor probe
    FURUMOTO Masatsugu; SATO Tatsuya; HASHIMOTO Mamoru; YASUI Takeshi; ARAKI Tsutomu
    バイオエンジニアリング講演会講演論文集, 15 Jan. 2001, The Japan Society of Mechanical Engineers, Japanese
    15 Jan. 2001 - 15 Jan. 2001
  • Molecule Vibrational Imaging by CARS microscopy
    HASHIMOTO Mamoru; INOUE Keigo; FUJITA Katsumasa; NAKAMURA Osamu; KAWATA Satoshi; ARAKI Tsutomu
    バイオエンジニアリング講演会講演論文集, 15 Jan. 2001, The Japan Society of Mechanical Engineers, Japanese
    15 Jan. 2001 - 15 Jan. 2001
  • Multi-spectral imaging by multi-focus CARS microscopy
    Proceedings of Multi-dimensional Microscopy 2001, 23, 2001
  • 1117 Change of Ca content in tissue of human blood vessel with aging and region
    ARAKI Tsutomu; TATEYAMA Yutaka; HASHIMOTO Mamoru; MASUDA Mitsuhiko; TOHNO Yoshiyuki
    関西支部講演会講演論文集, 16 Mar. 2000, The Japan Society of Mechanical Engineers, Japanese
    16 Mar. 2000 - 16 Mar. 2000, We have determined Ca contents in human vessels by Ar-microwave induced plasma atomic emission spectroscopy. 0n the basis of this measurement, we have studied relation between Ca content and age, and that of between Ca content and vessel region. Ca contents are femoral artery > thoracic aorta > internal jugular vein > superior vena cava > inferior vena cava. In the case of arterial tissue, Ca content increases with aging. Ca contents in umbilical artery and umbilical vein, which are used as the reference to aging and mechanical stress, are the same level as those in adult veins. Ca contents in monkey arteries are much smaller than those in human vessels, and the difference between Ca content in upper limb and that in lower one is not evident. In the case of human, however, Ca content in artery of lower limb is larger than that of upper one, and especially large in the part of elbow, thigh and knee. These results suggest that the effect of mechanical stress is one important factor for increase of Ca content in the arterial tissue.
  • Capillary Electrophoresis System using Biological Reaction of Single Cell as a Sensor Probe
    SATO Tatsuya; HASHIMOTO Mamoru; YASUI Takeshi; ARAKI Tsutomu
    The proceedings of the JSME annual meeting, 2000, The Japan Society of Mechanical Engineers, Japanese
    2000 - 2000, We have constructed a capillary electrophoresis (CES) system using living cells as the sensor tip of CES to monitor the change of cellar environment. In the present system, yeast cell stained with fluorescence dye was applied as the sensor cell. Fluorescence intensity of the probe cell changes according to the change of cellar environment. To confirm the performance of the proposed CES system, the pollutant (Hg^<2+>) and the biological substance (acetylcholine) were applied. Fluorescence intensity of Rhodamine123 (membrane potential sensitive dye for mitochondria), decreased gradually due to effect of the pollutant, while that of Fluo-3 (calcium sensitive dye) increased.
  • Changes of autofluorescence in human dentine caused by caries
    NAKAMOTO Kazunobu; YASUI Takeshi; HASHIMOTO Mamoru; ARAKI Tsutomu
    Proceedings of the JSME Bioengineering Conference and Seminar, 2000, The Japan Society of Mechanical Engineers, Japanese
    2000 - 2000
  • Effect of the detector side pinhole on 3D optical properties of CARS microscopy
    Proceedings of Focus on Microscopy 2000, 2000, English, Oral presentation
    [International presentation]
  • Molecular Vibrational Imaging in the Fingerprint Region by Cars Microscopy
    Proceedings of Focus on Microscopy 2000, p.~4, 2000
  • 同期式ナノ秒Xeアークランプ低ジッター化とその応用
    藤澤泰充; 橋本 守; 伊丹 伸; 荒木 勉
    中国四国支部第35期総会・講演会講演論文集, 1997, Japanese
    1997 - 1997
■ Syllabus
  • 生体情報工学特別演習, 2024年, 修士課程, 情報科学院
  • 医用システム工学特論, 2024年, 修士課程, 情報科学院
  • 生体情報工学特別研究, 2024年, 博士後期課程, 情報科学院
  • 医用システム工学特論, 2024年, 博士後期課程, 情報科学研究科
  • 医用システム工学特論, 2024年, 博士後期課程, 情報科学院
  • 情報エレクトロニクス概論, 2024年, 学士課程, 工学部
  • 卒業論文, 2024年, 学士課程, 工学部
  • 応用光学Ⅰ, 2024年, 学士課程, 工学部
  • 応用数学Ⅱ, 2024年, 学士課程, 工学部
  • 科学技術英語演習, 2024年, 学士課程, 工学部
  • 生体情報工学実験Ⅰ, 2024年, 学士課程, 工学部
  • 生体情報工学演習Ⅱ, 2024年, 学士課程, 工学部
  • 生体情報工学演習Ⅰ, 2024年, 学士課程, 工学部
  • 生体情報工学実験Ⅱ, 2024年, 学士課程, 工学部
  • 科学計測, 2024年, 学士課程, 工学部
■ Affiliated academic society
  • レーザ顕微鏡研究会
  • 日本光学会
  • 日本分光学会
  • 日本応用物理学会
  • 日本機械学会
■ Research Themes
  • Advanced non-invasive optical measurement techniques for the constant monitoring of blood lipid components
    Grants-in-Aid for Scientific Research
    01 Apr. 2023 - 31 Mar. 2026
    加藤 祐次; 清水 孝一; 松村 健太; 橋本 守
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 23K11799
  • Near-infrared light delivery in deep tissue using vivo helical optical waveguide formed by a topological ultrasound beam
    Grants-in-Aid for Scientific Research
    30 Jun. 2022 - 31 Mar. 2025
    橋本 守; 工藤 信樹; 加藤 祐次
    Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Pioneering), Hokkaido University, 22K18436
  • Development of coherent Raman scattering microendoscope and application to unstained in-situ diagnosis
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
    Apr. 2021 - Mar. 2024
    橋本 守; 七戸 俊明; 新岡 宏彦
    色素の静脈投与なしに癌を内視鏡検査で確定診断するために，上部消化管内視鏡(胃カメラ)の 鉗子孔に挿入可能な非線形ラマン散乱プローブ顕微内視鏡の開発を目指している．非線形ラマン散乱の一種であるコヒーレント反ストークスラマン散乱（CARS）を用いることで，無染色・高空間分解能・高速な細胞，細胞核の形状観測を可能とする．本年は，CARSプローブ顕微内視鏡におけるキーデバイスである，2波長板（DWP）の設計を行なった．DWPは，直交する偏光を持つ２波長の励起光を，互いに平行な偏光へと変換するデバイスである．このために，709 nmnの励起光に対してはリタデーションをゼロとし，888 nmの励起光に対してはλ/2となるように設計する必要がある．２波長に対してこのような特性を持つ波長板は，2種類の１軸性結晶を用いれば可能であるが高コストとなるため，1種類の結晶で設計可能かシミュレーションした．結晶には水晶を用い，発生するCARS光強度を最大となるように結晶の厚さを最適化したところ，0.1980 mmで，完全に平行な光を用いて励起した際の99%のCARS信号が得られることが分かった．また，同様に90%以上のCARS信号が得られるラマンシフト領域は，2,530 to 2,980 cm-1であることが分かった．したがって，このDWPでCH伸縮振動に対応する高波数領域のCARS信号を十分取得できることが分かった．また，励起光源としてファイバーレーザーベースの光源を作成し1550 nmでのパルス発振を確認できた．これまでに開発してきた原理検証システムを用いて，ポリスチレンビーズや培養細胞やラット組織の観測を行なった．ポリスチレンビーズの観測は行えたが，培養細胞や組織のCARS信号を得ることはできなかった．
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, Principal investigator, 21H04950
  • Development of transcutaneous optical measurement of blood particulate concentration for non-invasive monitoring of blood components
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Apr. 2020 - Mar. 2023
    加藤 祐次; 清水 孝一; 橋本 守; 松村 健太
    本研究の大きな目標である血中中性脂肪濃度の無侵襲光学計測の実現のため、計測手法は生体透過性の高い可視から近赤外の波長領域での後方散乱光の空間分解計測を基礎としている。前年度まで、計測対象の一つとしている前腕部静脈の数値モデルに対するモンテカルロ・シミュレーションにより、静脈の形状・位置に対して光源と検出器の距離を調整する必要性が分かったため、センサー配置の最適化について検討した。また、推定アルゴリズム精度のさらなる向上のため、静脈が走行していない箇所での周囲媒質のみの光学特性推定を組み合わせて、個人差や計測箇所による変動を抑えたロバスト性の向上を進めた。さらに、メディカルフォトニクス社の協力により試作機を用いて、これまで準備してきた実験ファントムを用いて実測し、シミュレーションや光拡散理論と比較検討を行った。その結果シミュレーションで検討してきた推定アルゴリズムに対して修正指針を得た。
    一方、毛細血管を対象とした光電式脈波計測法の応用について、スマートフォンアプリおよび多色のLEDとPDからなる計測モジュールを用い、前年度までの検討を踏まえて接触圧力をコントロールしつつ、脂肪食摂取前後の光電脈派信号の変化について評価した。その結果、現状では脂質濃度変化に対する光電脈波信号の変化は見出すことはできなかった。今後、脈波信号の解析について単純な信号強度の変化のみではなく、周波数解析等による解析を要することが課題となった。さらに、ハードウェア面で通常の光電脈波計測のみならず、干渉法に基づくスペックル計測法の適用等の計測法の拡張についても今後の課題とし、基礎実験データを得た。
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, Competitive research funding, 20K12685
  • 非線形ラマン散乱内視鏡実現のための4光波混合現象の削減法の開発
    研究助成
    Jun. 2020 - May 2021
    公益財団法人 村田学術振興財団, Principal investigator, Competitive research funding
  • Artificial intelligence pathological diagnosis by hyperspectral nonlinear Raman scattering imaging
    Grants-in-Aid for Scientific Research
    Apr. 2017 - Mar. 2020
    Hashimoto Mamoru
    By reducing the noise of observation data and improvement of observation time using deep learning, we succeeded in the imaging rate improvement of 1.6–1.2 image/min. to 12.5-4.0 image/min. In tissue classification by machine learning of nonlinear Raman scattering images, pre-training with fluorescence images significantly improved the segmentation ability. Besides, we have developed a new microscope for acquiring a large number of hyperspectral nonlinear Raman scattering images and succeeded in increasing the speed 14 times and reducing the excitation light peak irradiance 1/12 compared to the conventional method. It was shown that hyperspectral images of cultured cells were classified by deep learning, and that unsupervised learning could classify cells with different culture conditions.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 17H02793
  • Non-invasion observation of the cell culture process by nonlinear optical microscopy
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    01 Apr. 2015 - 31 Mar. 2019
    FUKUSHIMA Shuichiro
    The importance of controlling microscopic culture environment in cellular scale, was verified using microfluidic devices. By using an oxygen concentration controlled device, it was possible to realize the co-culture of the cells in different oxygen environment, which is difficult to control by the conventional methods. Moreover, the usefulness of nonlinear optical microscopy was shown as a method of non-invasion observation of the cell culture process. Visualization and deformation analysis of the substrate structure using second harmonic generation light are effective for the examination of the mechanical environmental factor, when the collagen gel is made to be culture substrate.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Osaka University, 15K01287
  • Super-resolution Imaging of nonlinear Raman atomic force imaging
    Grants-in-Aid for Scientific Research Challenging Research (Exploratory)
    Jun. 2017 - Mar. 2019
    Hashimoto Mamoru
    We have developed a super-resolution molecular vibration imaging microscope that combines atomic force microscopy (AFM) with nonlinear Raman scattering beyond the diffraction limit of light. The volume expansion accompanying the molecular vibrational excitation via nonlinear Raman scattering process is observed by AFM. In order to focus the laser beam near the probe of AFM, it is necessary to detect volume expansion with high sensitivity while irradiation of the probe. Therefore, we developed a system in which the frequency difference between the pulse oscillations of the two lasers is synchronized with the resonance of the cantilever so that the two pulse lights are simultaneously irradiated to the sample only when the probe contacts the sample. Using the developed device, we successfully detected a signal that seems to be volume expansion induced by nonlinear Raman scattering.
    Japan Society for the Promotion of Science, Challenging Research (Exploratory), Hokkaido University, Principal investigator, 17K19063
  • Elucidation of the glycated collagen reaction in dentin and its application to next dental treatment
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Apr. 2016 - Mar. 2019
    Miura Jiro; Araki Tsutomu
    Granular calcification is formed in the dental pulp of diabetic patients. Histopathologically, these are thought to be by-products of severe diabetes. In this study, the pathological calcification in dental pulp of Type2 diabetes model rat was evaluated using electron microscopy and immunohistochemical analysis of pentosidine, a kind of Advanced Glycation End products (AGEs) which is thought to be related calcification .The composition of pulp stone was examined with Energy Dispersive X-ray Spectoroscopy. In the type2 diabetes model rats, it was found that pathogenic calcification in pulp occurs with the high blood glucose level for 10 weeks in SDT fatty rats. Pentosidine which is a kind of AGEs strongly expresses in the pulp of diabetic rats. And granular calcification was also observed in the surrounding area of pulp stone. From these results, Glycation was related with calcification in dental pulp, suggesting that these are related to ectopic calcification in diabetic patients.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, Coinvestigator, 16H05517
  • Development of fluorescence lifetime measurement for glycation screening for diabetes in vivo
    Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
    01 Apr. 2016 - 31 Mar. 2018
    Miura Jiro
    Glycation, namely the Maillard reaction, is a non-enzymatic reaction between protein or lipid and reducing sugar, resulting in formation of advanced glycation end-products (AGEs). Because glycation of collagen progresses under physiological conditions in living organisms, glycation is one of the most important processes in human aging. Several AGEs bind collagen and act as cross-links between collagen fibrils, thereby changing the fluorescent lifetime of collagen-rich tissues. The purpose of this study is to identify AGEs in human dentin using electron microscopy, time-resolved fluorescence microscopy, mechanical testing and chemical analyses. Our findings suggest the potential influence of cross-linking on diabetic and aging tissue.
    Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Osaka University, 16K12877
  • Frequency-swept discrete Fourier transform spectroscopy ~demonstration and application in THz and near-infrared region~
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
    01 Apr. 2014 - 31 Mar. 2017
    YASUI TAKESHI
    In this research, we demonstrated improved spectral resolution by overcoming the time window size limitation using a mode-locked terahertz (THz) or near-infrared (NIR) pulse train as precisely periodic pulsed radiation in discrete Fourier transform spectroscopy (dFTS). Since infinitesimal resolution can be achieved at harmonic components of its repetition frequency 1/T when the time window size is exactly matched to the repetition period T, a combination of dFTS with a spectral interleaving technique achieves a spectral resolution only limited by the spectral interleaving interval.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), The University of Tokushima, 26246031
  • 高速誘導ラマン散乱スペクトルイメージングシステムの開発
    産学共創基礎基盤研究プログラム「ヒト生体イメージングを目指した革新的バイオフォ トニクス技術の構築」
    Dec. 2010 - Mar. 2017
    橋本 守
    科学技術振興機構, Principal investigator, Competitive research funding
  • Crystallization monitoring using higher order nonlinear Raman scattering microscopy
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    01 Apr. 2013 - 31 Mar. 2016
    Hashimoto Mamoru; FUKUSHIMA Shuichiro; NIIOKA Hirohiko
    We propose to use fourth order coherent Raman scattering (FOCRS) microspectroscopy for monitoring the crystallization process. The crystallization is a process in the saturated solution. The even order nonlinear phenomenon is prohibited from the solution and provides only the information of crystallized part. FOCRS also provides the information of crystal and the molecular information by the molecular vibration.
    We have developed a multiplex FOCRS microspectroscopy system using supercontinuum light generated by a photonic crystal fiber. We succeed in observation of FOCRS hyper-spectral image of a DAST (4-dimethylamino-N-methyl-4-stilbazolium tosylate) crystal in saturated solution. We also observed FOCRS and CARS (coherent anti-Stokes Raman scattering) hyper-spectral images of hydrated and anhydrated DAST crystals. CARS bands are clearly observed for both of crystals, but only the anhydrated DAST crystals show FOCRS bands. As a result, the selectivity of FOCRS was declared.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, Principal investigator, Competitive research funding, 25286070
  • Multimodal molecular imaging system and its application for investigation of in vivo cell/tissue
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
    01 Apr. 2011 - 31 Mar. 2014
    ARAKI Tsutomu; HASHIMOTO Mamoru; FUKUSHIMA Shuichiro; NIIOKA Hirohiko
    Aims of this research project are development and application of novel imaging method that can informs molecular morphology and function of biological specimens. In the present research, we have focused on the second harmonic generation (SHG) microscopy and cathode luminescence(CL) microscopy.A polarization resolved, high-speed SHG microscope was developed. In in vivo measurement with this microscope, age related dermis collagen image were observed. Also we demonstrated SHG imaging of tissue-engineered cartilage composed of rabbit chondrocytes and type I collagen gel. From this, we confirmed that it is suitable for evaluating the quality of tissue-engineered cartilage. In the CL microscopy, three kinds of rare-earth-doped nanophosphors were synthesized and injected into macrophages. The spatial distribution of nanophosphors was visualized by using a scanning electron microscope CL system.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Osaka University, 23240069
  • カソードルミネッセンス顕微鏡による細胞中の高空間分解能蛋白質イメージング
    Apr. 2011 - Mar. 2013
    橋本 守
    立石科学技術振興財団, Principal investigator, Competitive research funding
  • Realtime CARS imaging of cellular response by stimulation of laser ablation
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    2010 - 2012
    HASHIMOTO Mamoru; FUKUSHIMA Shuichiro; NIIOKA Hirohiko
    We have developed a multifocus coherent anti-Stokes Raman scattering microscopy system combined with a laser-beam ablation system. The developed system visualizes sample response by laser stimulation without staining at vide-rate. The laser beam for ablation is controlled independently of observation. The developed system was applied to cellular response by membrane disruption and crystallizationmonitoring by photon pressure.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, 22360027
  • Development and application of real-time molecular microscope based on non-linear optical effect for biological specimen
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
    2008 - 2010
    ARAKI Tsutomu; HASHIMOTO Mamoru; FUKUSHIMA Shuichirou; KINOーOKA Masahiro; IWATA Tetsuo; YASUI Takeshi
    Aims of this research project are to develop new microscopes utilizing non-linear optical effect induced by an ultra-short pulse laser and to observe molecular imaging of the biological specimen without unwanted staining treatment. We have developed a high-quality SHG microscope and CARS microscope, and following results have been obtained using these microscopes : (1) Age-dependency of collagen distribution in human skin was observed with the SHG microscope. This microscope was also applied to evaluate quality of cartilage cultured in collagen gel. (2) CARS image of Hela cell was demonstrated. Also repair of cell membrane was traced in time based on the CARS images.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Osaka University, 20240044
  • Nonlinear Raman microscopy to detect membrane protein
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    2007 - 2009
    HASHIMOTO Mamoru; FUKUSHIMA Shuichiro; FUJITA Katsumasa; YOSHIKI Keisuke
    We developed a multi-focus excitation nonlinear Raman microscope using a microlens array scanner for real-time molecular imaging. Parallel exposure of a specimen with light from two highly controlled ps mode-locked lasers (jitter of 30fs, point-by-point wavelength scan within 300ms) and parallel detection with an image sensor enabled real-time imaging. We demonstrated real-time Raman imaging of living cells (frame rate of 10fps). We also combined the developed system with the laser ablation to destruct the cell membrane, and demonstrated to observe the membrane repairing process. We added the polarization pattern control system to a nonlinear Raman microscopy system to detect membrane proteins by control the direction of electro-magnetic filed on the focus spot. The possibility of the selective detection of ordered molecules was confirmed with the imaging of liquid crystal.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, 19360033
  • サブミクロンの分解能で液晶分子の 3 次元配向を観測する顕微鏡
    平成 17 年度 第 2 回産業技術研究助成事業
    Jan. 2006 - Dec. 2008
    橋本 守
    新エネルギー・産業技術総合開発機構, Principal investigator, Competitive research funding
  • Nano-spectroscopy of molecular -metal complex
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    2007 - 2008
    INOUYE Yasushi; VERMA Prabhat; HASHIMOTO Mamoru; FUJITA Katsumasa
    金属ナノ構造に誘起される局在プラズモンは、光(フォトン)と共鳴的に結合することで、光をナノ領域に閉じ込め、さらに光電場を増強する。本研究では、この近接場ナノプラズモニクス技術を駆使することで、距離に強く依存した分子-金属原子間の相互作用を計測するナノラマン分光装置を開発し、さらにこれを用いて、距離に応じて分子とナノ探針先端の金属原子の間に電磁気学的、化学的、さらに力学的な相互作用が各々働くことを明らかにした。
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, 19360034
  • リアルタイム CARS 顕微鏡
    平成 18 年度稲盛財団研究助成金
    Apr. 2006 - Mar. 2007
    橋本 守
    稲盛財団, Principal investigator, Competitive research funding
  • テラヘルツ光照射に対する細胞応答のマルチフォトニクス・モニタリング
    科学研究費助成事業 萌芽研究
    2006 - 2007
    安井 武史; 福島 修一郎; 橋本 守; 荒木 勉; 東野 義之
    過渡的テラヘルツ光照射に対する細胞応答を観察する手段として利用する非同期光サンプリング式テラヘルツ・ポンプ・プローブ法において、テラヘルツ・スペクトルの広帯域化を実現するため、2台の極超短パルスレーザー(パルス幅10fs)を用いた非同期光サンプリング光源を開発した。また、2台のレーザーのモード同期周波数及び両レーザーのモード同期周波数差の高安定化に取り込み、従来光源と比較して10倍以上の高安定化を達成した。そして、本光源を用いた非同期光サンプリング式テラヘルツ時間領域分光装置で低圧水蒸気ガスのスペクトル計測を行い、本装置のスペクトル分解能が7GHzであることを確認した。テラヘルツ・ポンプ・プローブ法で細胞応答を観察するためにはスペクトル分解能がまだ不足しており、レーザー制御手法を再検討し、さらなる高分解能化をはかる必要がある。
    定常的テラヘルツ照射に対する細胞内応答の観察のために、アクティブ周波数逓倍器と周波数シンセサイザーを用いた連続発振(CW)テラヘルツ光源を開発し、パワー10mW、スペクトル線幅1Hz以下、周波数安定度10-11、チューニング範囲75GHz〜110GHzの基本特性を得た。一方、生体SHG(第2高調波発生光)イメージングは細胞内コラーゲン産生に関する情報を抽出するために利用する。今年度は、深部プローブ型SHGイメージング装置(レーザー波長1250nm)及び高分解SHGイメージング装置(レーザー波長800nm)を様々な生体サンプルに適用し、その有用性を評価した。その結果、細胞培養コラーゲンゲル、光老化真皮、心臓弁膜症によるコラーゲン変性、軟骨表層部、などの観察に有用であることが分かった。
    日本学術振興会, 萌芽研究, 大阪大学, 18650121
  • 擬似ランダム符号を用いたパルスシェーピング多焦点CARS顕微鏡
    科学研究費助成事業 萌芽研究
    2006 - 2007
    橋本 守
    生きたままの生体組織・細胞を構成する分子ならびにその機能を観測する"分子イメージング"技術は,ポストゲノム研究において必要不可欠な研究ツールとなる.CARS(コヒーレントアンチストークスラマン散乱)顕微鏡は,全ての分子が持つ分子振動を観測することにより,無染色に分子種ならび分子構造情報を得ることができ,また非線形光学効果による回折限界を超えた空間分解能と誘導放出過程による高い感度を備えた観測手段である.しかしながら,これまでのCARS顕微鏡では2色のレーザーを必要としていたために,装置が複雑なものとなっていた。本研究では,一台の超短パルスレーザーを使用し,パルスシェーピングすることによって選択的に分子振動を励起する手法の開発を行った.
    昨年度,作製したパルスシェーピング装置を改造し,2ビームを同時にパルスシェーピング可能なえるものとした.これにより,相互相関を容易に観測できるようになった.パルスシェーピング時に与える変調方法の比較を行った.正弦波状の位相変調を与えるものと,擬似ランダム符号を与えた時との比較を行ったところ,擬似ランダム符号を与えた方が,相互相関信号のパルス列の数が多くなる(正弦波では3-5本,擬似ランダム符号では9-13本)ことが確認できた.また,四塩化炭素を試料にCARS信号を観測した結果,分子振動が選択的に励起(周波数分解能が約3倍に向上)されていることが確認できた.
    日本学術振興会, 萌芽研究, 大阪大学, 18656019
  • Development of staining-free molecular imaging microscope for biological molecules and its application for quality control of cultured cells
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
    2005 - 2007
    ARAKI Tsutomu; HASHIMOTO Mamoru; KINO-OKA Masahiro; YASUI Takeshi; IWATA Tetsuo; TOHNO Yoshiyuki
    Aims of this research project are to develop new microscopes by which molecular imaging of the biological specimen can be obtained without unwanted staining treatment, and to apply these microscopes for quality control of the cultured cells. The results are written as follows.
    (1) We have developed a high-speed SHG microscope and measured in situ tomographic images of collagen molecules in human dermis through the skin. The measurement time to obtain one image was shortened to one second that value is about 1/60 of the conventional equipment.
    (2) We have developed another SHG microscope with a controlled polarization pattern to detect 3D / molecular orientation. Using this microscope, we have demonstrated the 3D collagen profiles in Achilles tendon.
    (3) Deformation and remodeling of collagen gel due to endotherial cells have been observed by SHG microscopy in order to apply that microscopy for quality control of the cell culture.
    (4) We have developed a high-speed CARS microscopy system. Using this system, a real-time imaging of liposomes has been demonstrated.
    (5) We have measured calcium contents in various vascular walls and analyzed the heterogeneity of the contents based on biomechanical effect.
    (6) A new quantification technique for calcium ion concentration has been proposed based on fluorescence lifetime measurement. The waveform distortion caused by the frequency band width limitation of the instruments has been compensated by a newly proposed algorithm.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Osaka University, 17200032
  • 分子のベクトル分布観測顕微鏡の開発
    平成 17 年度研究助成
    Sep. 2005 - Aug. 2006
    橋本 守
    伊藤科学振興会, Principal investigator, Competitive research funding
  • 2 光子検出器を用いた二台のピコ秒モードロックレーザーの高精度同期
    第 21 回 (平成 17 年度) 研究助成
    Jun. 2005 - Aug. 2006
    橋本 守
    村田学術振興財団, Principal investigator, Competitive research funding
  • ピコ秒レーザーの自動安定発振・パルス長最適化機構の開発
    シーズ育成試験
    Jan. 2006 - Mar. 2006
    橋本 守
    科学技術振興機構, Principal investigator, Competitive research funding
  • 非線形光学効果による赤外発生とプローブレス近接場赤外顕微鏡への展開”
    若手研究 A
    Apr. 2003 - Mar. 2005
    橋本 守
    日本学術振興会, Principal investigator, Competitive research funding
  • Van der Waals'force-controlled nano Raman scattering spectroscopy
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    2004 - 2005
    INOUYE Yasushi; HASHIMOTO Mamoru; FUJITA Katsumasa
    We found spectral shift of specific Raman band of carbon nanotubes (CNTs) when van der Waals's force is applied onto the CNTs. We measured Raman spectrum of single CNT bundle while applying stress on it with silver coated cantilever tip of atomic force microscope, and observed spectral change of Raman band of CNT in situ. When we apply stress from 0nN to 2.4nN successively, lower peak of G-band (〜1600cm-1) was red-shifted by 18cm-1. G-band reflects electric property of CNT and has two adjacent peaks. On the other hand, no peak shift was observed in higher peak of G-band. This phenomenon originates from the fact that two peaks are corresponding to the vibrational modes of different vibrational direction, and these two vibrational modes have different shearing stresses with respect to each other. Furthermore, we observed peak shift of radial breathing mode (RBM) around 200cm-1〜300cm-1 at most 5cm-1. RBM depends on the diameter of CNT. This peak shift can be attributed to the fact that the cross-section of CNT is deformed into ellipse shape by applied uniaxial stress on the CNT. We also observed intensity of all Raman band are enhanced when applying stress. This suggests that the band gap energy of CNT comes near to the resonance energy(2.33eV) because of the deformation of CNT along the long axis of CNT, thus contribution of resonant Raman effect is increased.
    We also performed vibrational calculation of molecule-metal cluster including a number of metal atom based on density functional theory. The calculation showed the experimentally observed band shift by complex formation mechanism is well reproduced if the metal cluster contains at least four Ag atoms.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, 16360034
  • 3次元偏光制御顕微鏡による3次元分子配向の3次元観測
    科学研究費助成事業 萌芽研究
    2004 - 2005
    橋本 守; 安井 武史; 福島 修一郎
    従来の光学顕微鏡技術では,高い空間分解能を持ちながら3次元的な分子の配向を観測することは不可能であった.これは,通常の直線偏光ビームを集光しても焦点での電場の向きは光軸に対して直行しているために,光軸方向に向いた分子を観測することはできないためである.一方,ラジアル偏光と呼ばれる放射状の偏光を高い開口数を持つ対物レンズで集光すると焦点で光軸方向の電場が形成されるために光軸方向に向いた分子も観測することができることが知られている.したがって,レーザー走査顕微鏡においてレーザービーム断面内の偏光分布を自由に制御し,直線偏光やラジアル偏光を高速に変化させることができれば焦点の電場の方向を3次元的に変化させることができる.また,この偏光分布制御と非線形光学効果を組み合わせることで,6次元観測(3次元空間+3次元配向)することが可能となる.
    フェムト秒超短パルスレーザー光を,平行配向した液晶からなる液晶空間位相変調素子(SLのM)を2回通すことにより,任意の空間的偏光分布を形成し,この光を励起光に用いた第二高調波発生顕微鏡を構築した.SLMに印加するパターンを変え,直交した一様な偏光直線偏光ビームと,ラジアル偏光を試料に入射し,その第二高調波発生を観測することで,試料の分子配向を観測することが可能かどうか検証した.試料には,コラーゲンを主成分とし,コラーゲン線維が一方向に並んだヒトアキレス腱を用いた.コラーゲンは線維方向と入射電場の方向が一致した場合に強い第二高調波を発生することが知られているが,光軸方向にコラーゲン線維が向いた試料を開発した装置で観測したところ,ラジアル偏光励起で強い信号が得られ,提案手法の原理確認を行うことができた.
    日本学術振興会, 萌芽研究, 大阪大学, 16656024
  • Development of molecular imaging microscope for biological specimen and its application for tissue examination
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    2003 - 2004
    ARAKI Tsutomu; HASHIMOTO Mamoru; YASUI Takeshi; TOHNO Yoshiyuki; IWATA Tetsuo
    Aim of this research project is to develop an effective method that can sense objective biomolecles without staining and to apply this method to identification of the situation of the molecles. For ths purpose, we have focused on non-linear optical effect in the tissue that is induced by the irradiation with high-intensity very short duration pulsed laser light such as a femtosecond laser. In the actual research, we have developed three microscopes ; fluorescence lifetime imaging microscope in nanosecond range, a collagen sensitive microscope using second harmonic generation light (SCG light) and a coherent anti Stokes coherent Roman scattering (CARS) microscope.
    The most successful one is the SHG microscope. Based on the reflection-type microscope with a probe light spot of 10 micrometers in diameter and the polarization measurement of SHG light induced by collagen molecules, we have determined distribution of collagen fiber orientation in various human tissues. The resulting SHG data in human skin imply that the reticular dermis posses a tangled structure of collagen fibers which is consistent with the result of the anatomical examination of the skin. We have improved the SHG microscope to obtain 3D dissection image using the con-focal optics and the 3D sample scanning stage. With this microscope, tomograph of the collage fiber orientation in the human skin and bone have been measured.
    Performance of the fluorescence lifetime imaging microscope was demonstrated by the measurement of the autofluorescence in liposome. Also we have measured the distribution of hyaluronic acid around an erythrocyte with CARS microscope.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, 15300161
  • 非線形光学効果による赤外発生とプローブレス近接場赤外顕微鏡への展開
    科学研究費助成事業 若手研究(A)
    2003 - 2004
    橋本 守
    近年のナノテクノロジーの発達に伴い,高い空間分解能を持ちながら微小物体の組成や分子構造を観測する手法の開発が望まれている.すべての分子は分子振動を有し,その周波数は分子構造に非常に敏感であるため,分子振動を観測する振動分光による高空間分解能観測に関する研究を行った.
    赤外光をそのまま集光しても回折限界により波長程度までしか集光できないために,赤外顕微鏡の空間分解能は10ミクロン程度に限定される.しかしながら,可視あるいは近赤外光を用いて赤外光を発生させることができたなら,可視光あるいは近赤外光の波長程度の空間分解能を持った顕微鏡を構成することができる.大気中でレーザー光を集光して誘電破壊(レーザーブレイクダウン)により発生するマイクロプラズマを光源とした近接場顕微鏡の開発を目指して,マイクロプラズマの発生,観測に関する研究を行った.高強度なフェムト秒レーザーをNA=0.6の対物レンズで強く集光することにより,直径2ミクロン程度のマイクロプラズマを生成することに成功した.また,このプラズマから1000cm^<-1>から4000cm^<-1>に渡る赤外光が放射されていることを実験的に確認した.しかし,本手法を近接場へと展開した場合,プラズマにより試料が破壊されてしまった.そこで,金属薄膜での非線形光学効果を利用した波長変換技術を用いることを検討し,金属薄膜での差周波発生の確認を行った.
    また,コヒーレントアンチストークスラマン散乱(CARS)と呼ばれる非線形光学現象を,近接場顕微鏡へと適用した.これは,金属プローブ先端付近での電場増強効果と非線形光学効果による相乗効果により従来にない高空間分解能化した振動分光を行うことであり,波長約800nmの光を用いて15nmの空間分解能と,回折限界の数十分の1まで向上させることに成功した.光を用いた振動分光において従来にない空間分解能を達成することができた.
    日本学術振興会, 若手研究(A), 大阪大学, 15686003
  • 紫外励起 CARS 顕微鏡のシステムの開発と生物組織観察への応用
    基盤 B 展開
    Apr. 2001 - Mar. 2003
    橋本 守
    日本学術振興会, Principal investigator, Competitive research funding
  • 多焦点 CARS 顕微鏡による 3 次元分子分布イメージング
    奨励研究 A
    Apr. 2001 - Mar. 2003
    橋本 守
    日本学術振興会, Principal investigator, Competitive research funding
  • Development and application of a novel capillary chromatography system utilizing vital reaction of a single cell
    Grants-in-Aid for Scientific Research
    2001 - 2002
    ARAKI Tsutomu; TOHNO Yoshiyuki; YASUI Takeshi; HASHIMOTO Mamoru; CHIKAMORI Kensuke
    We have proposed a biosensor chromatography system in conjunction with a capillary electrophoresis (CE) chromatography and a living cell to monitor the cellular environment. As the sensor cell for bio-CE system, Saccharomyces cerevisiae (SC) was used, SC was stained with rhodamine 123 (Rhl23) that is sensitive to electric potential of the mitochondria membrane. The cell was enveloped inside the capillary, and resultant Rh123 fluorescence image was measured with a fluorescence microscope and a CCD system.
    In order to avoid unwanted electric field of 5 kV to the sensor cell, a micro-crack that works as the electric by-pass is attractive. To make the by-pass, micro-processing by a femtosecond (fs) laser was applied We have achieved to hole the glass wall of the capillary tube with a few micrometer in diameter, however the processing has not completed yet because of lack of the precision for the laser beam focusing inside the small wall. Further trial is required to succeed in the production of the micro hole. When the pulsed light from the fs laser is irradiated to the biological tissue, second harmonic generation light (SHG light) is induced. Based on this phenomenon, we have constructed a polarization SHG light detection system. We found that orientation of the collagen fiber has been informed by the SHG light.
    As another trial of the cell sensor system, we have utilized a Paramecium caudatum as the sensor cell to monitor the water pollution based on the analysis of the cell moving profile in a micro-chamber. The paramecium cell showed characteristic moving profiles due to the pollutant such as heavy metal ions and acid. We have also studied the emission characteristics of a white light emitting diode to evaluate applicability to the compact light source in the present sensor system.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, 13480294
  • コヒーレントアンチストークスラマン散乱による生体組織の3次元局所空間分子分光分析
    第16回研究助成
    Apr. 2000 - Mar. 2001
    橋本 守
    仲谷電子計測技術財団, Principal investigator, Competitive research funding
  • コヒーレントアンチストークスラマン散乱顕微鏡の開発と生体計測への応用
    奨励研究 A
    Apr. 1999 - Mar. 2001
    橋本 守
    日本学術振興会, Principal investigator, Competitive research funding
  • Development of photometric detection system for tissue aging and study the localization of the aging in tissue
    Grants-in-Aid for Scientific Research
    2000 - 2001
    ARAKI Tsutomu; TOHNO Yoshiyuki; YASUI Takeshi; HASHIMOTO Mamoru; YAMADA Gen
    The aim of this project is to develop an effective photometry system that can detect tissue aging of living body and to study the localization of tissue aging. The results of the project are described in the following.
    (1) A time-resolved confocal-microscope system with subnanosecond-resolution has been developed. Three-dimensional kinetic fluorescence image can be measured with this system. Image of calcium concentration in an yeast cell has been demonstraed based on the fluorescence lifetime image measurement.
    (2) Fluorescent collagen has been extracted from human dentin using acetic acid. The fluorescence spectra of the tooth-extracted collagen was consistent with that of the dentin tissue. Also the fluorescence intensity of the tooth-extracted collagen increased with the age, and with reaction time in the ribose solution. We have concluded that the main fluorophore of the age dependent fluorescence emission is AGE (Advanced Glycosylation Endproduct) that is generated in tissue collagen by the reaction with reducing sugar.
    (3) The second-harmonic generation light (SHG-light) detection system has been constructed. SHG-light is sensitive to tissue collagen conformation. We found that orientation of the collagen fiber has been informed by the SHG light.
    (4) Calcium content at bifurcation of the human brachial artery has been measured with an Ar-microwave-induced plasma emission spectrophotometer, and a possible correlation between Ca content and mechanical property of the artery wall has been considered. We have found that the portions in which Ca deposition is significant are the regions of low wall shear stress, and concluded that arteriosclerosis occurs in the regions of low wall share stress.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, 12558108
  • コヒーレントアンチストークスラマン散乱顕微鏡による生体試料の3次元イメージング
    Nov. 1999 - Oct. 2000
    橋本 守
    住友財団基礎科学研究助成, Principal investigator, Competitive research funding
  • 細胞の持つ優れた分子選択能力を活用したバイオセンサー型クロマトグラフィの開発
    科学研究費助成事業
    1999 - 2000
    荒木 勉; 橋本 守
    細胞の環境感知能力と光学的手法による情報・収集技法を併用した生物マイクロセンサー方式のキャピラリー電気泳動システムの開発を目的としている。細胞に薬物や環境汚染物質などの刺激が加わるとミトコンドリア膜電位が変化したり、細胞質にカルシウムが放出されることに着目した。具体的には、細胞刺激物質の検出をCaを指標にして行う。センサー細胞として前年度には酵母菌を用い、Ca指示蛍光色素であるFluo-3で染色したが、キャピラリー両端にかかる電界(約150V/cm)によって蛍光強度が漸減するため、応用性に問題があった。そこで今年度は2波長励起による比測定法を採用した。そのための専用の顕微蛍光測光システムを製作した。センサー細胞には人の白血球を用いた。全血より白血球を分離し、Ca指示色素Fura-2で染色する。細胞を長さ31cm,内径25μmのガラス管(キャピラリー)内の中央付近に付着させ、キャピラリー両端に2-10kVの直流電圧をかける。はじめに蛍光強度の電界依存性を調査したが、印加電圧が4.5kVまでは影響がなかった。次にサンプル槽にアセチルコリンを注入すると、4.5kVの電圧ではアセチルコリンが約7分かかって細胞まで到達し、細胞を刺激する。これによって細胞内Caが上昇して、蛍光信号が得られた。その際、2波長情報からCa濃度が定量できた。さらにMgイオンについても、その検出のためにMag-fura-2の染色を試みた。以上の結果より、細胞をセンサーチップとした新しい電気泳動システムが実現可能であることがわかった。これらの結果を日本機械学会にて発表した。
    日本学術振興会, 萌芽的研究, 大阪大学, 11875063
  • Detection of tissue aging by nanosecond self-fluorescence caused by AGE (Advanced Glycosylation Endproduct)
    Grants-in-Aid for Scientific Research
    1999 - 2000
    TSUTOMU Araki; GEN Yamada; YOSHIYUKI Tohno; MAMORU Hashimoto
    The aim of this project is to develop photometry system that can detect tissue aging of living body. The results of the project are described in the following.
    (1) The tissue fluoresces blue light by illumination with UV-light. The observed fluorescence decay time was in nanosecond range when illuminated with impulse UV-light. On the human dentine and human arterial tissue, the fluorescence intensity increased and fluorescence decay time decreased with increase of age, respectively. These results show that tissue fluorescence can be used as an effective indicator for aging.
    (2) The fluorescence decay curve had two decay components whose lifetimes are 2.8 ns and 9 ns. The intensity of 2.8 ns component showed strong age dependency, but 9 ns one showed weak dependency
    (3) We assumed that the main fluorophore of the age dependent fluorescence emission is AGE (Advanced Glycosylation Endproduct) that is generated in tissue collagen by the reaction with reducing sugar. To confirm this, type I collagen plate, human dentin section, rat tail tendon were incubated with ribose solution. Resultant fluorescence intensity and fluorescence decay time increased and decreased, respectively, with increase of the incubation time. The 2.8 ns component more increased than the 9 ns one.
    (4) To inform the change of collagen characteristics due to AGE, a new technique that can visualize the conformation change of molecules in living sample has been developed. This technique is based on the coherent anti-Stokes Raman spectroscopy (CARS). To establish the microscopic CARS method, coherent and optical transfer functions of the CARS system have been studied.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B)., Osaka University, 11480257
  • Visualization and quantification of intracellular magnesium ion to determine the influence of magnesium to pragnancy circulation
    Grants-in-Aid for Scientific Research
    1998 - 1999
    ARAKI Tsutomu; HASHIMOTO Mamoru; YAMAZAKI Mineo; TOHNO Yoshiyuki
    One aim of this project is to develop a microphotometric measurement system that can visualize and quantify the intracellular magnesium ion level, and to apply this system into the study on the dynamics of magnesium ion during the pregnancy circulation. To visualize the intracellular magnesium ion, a fluorescent probe (Mag-fra2) is used in conjunction with a micro spectrophotometer that has two excitation wavelengths to take an intensity ratio of the fluorescence emission. Because the calcium ion level and the magnesium ion level is competetive in the cell, both calcium level and magnesium level must be measured with the same apparatus. The test apparatus has been completed. Relationship between the magnesium ion level in thrombocyte and the pregnancy has been investigated in preeclamptic pragnant women.
    The project has been extended to quantify the calcium in human blood vessels. The calcium is quantified by atomic emission spectroscoy (AES). For this, a microwave-induced coupled AES system has been made. The calcium content of lower limbs was higher than that of upper limbs. This result indicates that correlation between Ca content and arteriosclerosis maybe exists. Ca was much contained in parts of veinlet and joint in the artery comparing with other portion. This indicates that correlation between Ca content and mechanical stress such as exercise (living custom), blood pressure, and blood turbulent flow maybe exists.
    Also a new technique that can visualize the conformation change of molecules in living sample has been developed. This technique is based on the microscopic optics and coherent anti-Stokes Raman spectroscopy (CARS). To establish the microscopic CARS method, optical transfer function of the CARS system has been studied.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Osaka University, 10558134
■ Industrial Property Rights
  • Polarization control device and polarization control method
    Patent right, Keisuke Yoshiki; Mamoru Hashimoto, University of Hyogo
    特願2016-230675, 28 Nov. 2016
    特開2018-87885, 07 Jun. 2018
    特許6817623
    20 Jan. 2021, 公立大学法人兵庫県立大学
  • 変調光検出のＳＮ比を向上する方法
    Patent right, 橋本 守, 川人 祥二, 国立大学法人静岡大学, 国立大学法人大阪大学
    特願2014-254326, 16 Dec. 2014
    特開2016-114519, 23 Jun. 2016
    特許6425242
    21 Nov. 2018, 国立大学法人静岡大学,  国立大学法人北海道大学
  • スペクトル分解能とスペクトル確度を向上するフーリエ変換型分光法、分光装置および分光計測プログラム
    Patent right, 安井 武史; 橋本 守; 荒木 勉; 弥永 祐樹, 国立大学法人大阪大学
    特願2014-532784, 26 Aug. 2013
    特許6032574
    30 Nov. 2016, 国立大学法人大阪大学
  • カソードルミネッセンス用標識試薬
    Patent right, 新岡 宏彦; 橋本 守; 古川 太一, 国立大学法人大阪大学
    特願2012-121380, 28 May 2012
    特開2013-246104
    特許5984112
    06 Sep. 2016, 国立大学法人大阪大学
  • 波長走査パルス光同期システムおよびその制御方法
    Patent right, 橋本 守; 丸山 真幸, 国立大学法人大阪大学, 株式会社メガオプト
    特願2012-1578, 06 Jan. 2012
    特開2013-142552, 22 Jul. 2013
    特許5926055
    25 May 2016, 国立大学法人北海道大学, Domestic, Domestic
  • 誘導ラマン散乱顕微鏡
    Patent right, 橋本 守, 国立大学法人大阪大学
    特願2011-259619, 28 Nov. 2011
    特開2013-113689, 10 Jun. 2013
    特許5831901
    09 Dec. 2015, 国立大学法人北海道大学
  • パルスレーザ光のタイミング調整装置，調整方法及び光学顕微鏡
    Patent right, 橋本 守, 南川 丈夫, 谷本 尚生, 小林 実, 藤田 克昌, 河田 聡, 荒木 勉, 国立大学法人大阪大学
    特願2008-515426, 20 Nov. 2006
    特許4862164
    25 Jan. 2012, 北海道札幌市 国立大学法人北海道大学
    特願2006-135293

Research Profiles

■ Research Profiles