Researcher Profile and Settings

Affiliation

• Field Science Center for Northern Biosphere　Agro-Ecosystem Research Station　Experiment Farm

Job Title

• Professor

Degree

• MS, agriculture（Kyushu University）
• Ph.D, agriculture（Kyushu University）

J-Global ID

• 200901028003354555

Research Interests

• Beef direct-marketing   Wagyu   metabolic programming   生産システム   産肉生理   Animal functional anatomy   IoT system for grazing   Meat-productive Physiology   Functional Anatomy   

Research Areas

• Life sciences / Veterinary medicine
• Life sciences / Animal production science

Academic & Professional Experience

• 2022/12 - Today Hokkaido University Field Science Center for Northern Biosphere Professor
• 2020/10 - Today 日本学術会議 第25-26期 日本学術会議連携会員
• 2017/05 - Today Kyushu University Graduate school of Agriculture Guest Professor
• 2017/05 - Today Kagoshima University Department of Agricultural Sciences and Natural Resources, Faculty of Agriculture Professor
• 2022/12 - 2023/03 Kagoshima University Faculty of Agriculture
• 2007/04 - 2017/04 九州大学大学院農学研究院 准教授
• 2000/08 - 2007/03 九州大学大学院農学研究院 助教授
• 1997 - 2000 Kyushu University Faculty of Agriculture
• 1997 - 2000 Kyushu University, Faculty of Agriculture,
• 2000 - 九州大学大学院農学研究院 助教授
• 2000 - Kyushu University, Faculty of Agriculture,
• Associate Professor
• Assistant Professor

Education

•        - 1996  Kyushu University
•        - 1996  Kyushu University  Graduate School, Division of Agriculture
•        - 1988  Kyushu University  School of Agriculture
•        - 1988  Kyushu University  Faculty of Agriculture

Association Memberships

• ASAS(American Society of Animal Science)   EAAP（European Federation of Animal Science）   エピジェネティクス研究会   The Society of Farm Animal in Infectious Diseases   Society of Beef Cattle Science   International Society of Developmental Origins of Health and Disease   The Japan Society for DOHaD   The Japan Society for Sustainable Meat Production   Warm Regional Society of Animal Science, Japan   Japan Society of Animal Nutrition and Metabolism   日本農芸化学会西日本支部   Japan Society for Meat Science and Technology   日本草地学会   日本分子生物学会   The Japan Society for Comparative Endocrinology   日本畜産学会   

Research Activities

Published Papers

• DNA Methylation of Genes Participating in Hepatic Metabolisms and Function in Fetal Calf Liver Is Altered by Maternal Undernutrition during Gestation.

  Susumu Muroya, Konosuke Otomaru, Kazunaga Oshima, Ichiro Oshima, Koichi Ojima, Takafumi Gotoh

  International journal of molecular sciences 24 (13) 2023/06/26 

  This study aimed to elucidate the effects of maternal undernutrition (MUN) on epigenetic modification of hepatic genes in Japanese Black fetal calves during gestation. Using a previously established experimental design feeding the dams with 60% (LN) or 120% (HN) of their global nutritional requirements during the 8.5-month gestational period, DNA methylation in the fetal liver was analyzed with reduced representation bisulfite sequencing (RRBS). The promoters and gene bodies in the LN fetuses were hypomethylated compared to HN fetuses. Pathway analysis showed that the genes with DMR in the exon/intron in the LN group were associated with pathways involved in Cushing syndrome, gastric acid secretion, and aldosterone synthesis and secretion. Promoter hypomethylation in the LN group was frequently observed in genes participating in various signaling pathways (thyroid hormone, Ras/Rap1, PIK3-Akt, cAMP), fatty acid metabolism, and cholesterol metabolism. The promoter hypomethylated genes ALPL and GNAS were upregulated in the LN group, whereas the promoter hypermethylated genes GRB10 and POR were downregulated. The intron/exon hypomethylated genes IGF2, IGF2R, ACAD8, TAT, RARB, PINK1, and SOAT2 were downregulated, whereas the hypermethylated genes IGF2BP2, NOS3, and NR2F1 were upregulated. Collectively, MUN alters the promoter and gene body methylation of genes associated with hepatic metabolisms (energy, cholesterol, mitochondria) and function, suggesting an impact of altered gene methylation on the dysregulation of gene expression in the fetal liver.
• The successful use of -omic technologies to achieve the ‘One Health’ concept in meat producing animals

  Sabine Farschtschi, Irmgard Riedmaier-Sprenzel, Ouanh Phomvisith, Takafumi Gotoh, Michael W. Pfaffl

  Meat Science 193 108949 - 108949 0309-1740 2022/11
• Maternal Nutrient Restriction Disrupts Gene Expression and Metabolites Associated with Urea Cycle, Steroid Synthesis, Glucose Homeostasis, and Glucuronidation in Fetal Calf Liver

  Susumu Muroya, Yi Zhang, Kounosuke Otomaru, Kazunaga Oshima, Ichiro Oshima, Mitsue Sano, Sanggun Roh, Koichi Ojima, Takafumi Gotoh

  Metabolites 12 (3) 203 - 203 2022/02/24 [Refereed]
   

  This study aimed to understand the mechanisms underlying the effects of maternal undernutrition (MUN) on liver growth and metabolism in Japanese Black fetal calves (8.5 months in utero) using an approach that integrates metabolomics and transcriptomics. Dams were fed 60% (low-nutrition; LN) or 120% (high-nutrition; HN) of their overall nutritional requirements during gestation. We found that MUN markedly decreased the body and liver weights of the fetuses; metabolomic analysis revealed that aspartate, glycerol, alanine, gluconate 6-phosphate, and ophthalmate levels were decreased, whereas UDP-glucose, UDP-glucuronate, octanoate, and 2-hydroxybutyrate levels were decreased in the LN fetal liver (p ≤ 0.05). According to metabolite set enrichment analysis, the highly different metabolites were associated with metabolisms including the arginine and proline metabolism, nucleotide and sugar metabolism, propanoate metabolism, glutamate metabolism, porphyrin metabolism, and urea cycle. Transcriptomic and qPCR analyses revealed that MUN upregulated QRFPR and downregulated genes associated with the glucose homeostasis (G6PC, PCK1, DPP4), ketogenesis (HMGCS2), glucuronidation (UGT1A1, UGT1A6, UGT2A1), lipid metabolism (ANGPTL4, APOA5, FADS2), cholesterol and steroid homeostasis (FDPS, HSD11B1, HSD17B6), and urea cycle (CPS1, ASS1, ASL, ARG2). These metabolic pathways were extracted as relevant terms in subsequent gene ontology/pathway analyses. Collectively, these results indicate that the citrate cycle was maintained at the expense of activities of the energy metabolism, glucuronidation, steroid hormone homeostasis, and urea cycle in the liver of MUN fetuses.
• Maternal Nutrition During Gestation Alters Histochemical Properties, and mRNA and microRNA Expression in Adipose Tissue of Wagyu Fetuses

  Yi Zhang, Konosuke Otomaru, Kazunaga Oshima, Yuji Goto, Ichiro Oshima, Susumu Muroya, Mitsue Sano, Sanggun Roh, Takafumi Gotoh

  Frontiers in Endocrinology 12 2022/02/01 [Refereed]
   

  We hypothesized that maternal low or high nutrition would give unique effects to morphological and molecular dynamics in adipose tissue of fetus of fatty breed Wagyu (Japanese Black) cattle which produce highly marbled beef. This study aimed to determine the effects of maternal energy intake in Wagyu cows, during gestation on fetal adipose tissue development, histochemical properties, and gene and microRNA (miRNA) expression. Cows were allocated to one of two nutritional energy groups: 120% (HIGH) or 60% nutritional requirements of (LOW). Fetuses (n = 6 per treatment) were removed from pregnant cows by cesarean section at fetal age 260 ± 8 days and euthanized. Subcutaneous adipose tissue (SAT), thoracic cavity visceral adipose tissue (TVAT), and perirenal adipose tissue (PAT) were collected for analysis. In histochemical analysis, in SAT and PAT, HIGH fetuses had greater diameter of adipocytes than LOW fetuses (P<0.05). Only in SAT, LOW fetuses had more Leptin (LEP) mRNA and tended to have more Peroxisome Proliferator-Activated Receptor gamma (PPARG) CCAAT-enhancer-binding proteins alpha (CEBPA) and Glucose transporter (GLUT) 4 mRNA(P<0.10). In all SAT, TVAT, and PAT, LOW fetuses had higher levels of the brown adipose tissue (BAT) biomarkers Uncoupling Protein (UCP) 1 and PPARG coactivator (PGC) 1α mRNA than HIGH fetuses (P<0.08). Meanwhile, in the other adipose tissue, LOW fetuses had lower PPARG, CEBPA, and Zinc Finger Protein (ZFP) 423 (in TVAT and PAT), FASN (in TVAT), LEP and GLUT4 mRNA (in PAT; P<0.10). In particular, in TVAT and PAT, LOW fetuses exhibited lower expression of WAT biomarkers (PPARG and ZFP423). Differential expression of various miRNAs related to adipogenesis between the LOW and HIGH fetuses was detected in an adipose tissue-specific manner (P<0.10). Based on adipose tissue-specific effects of maternal nutrition, these findings suggested that poor maternal nutrition in Wagyu cattle increased BAT development in SAT, TVAT and PAT, while elevated maternal nutrition stimulated fetal SAT development compared with that of TVAT and PAT.
• Maternal Undernutrition during Pregnancy Alters Amino Acid Metabolism and Gene Expression Associated with Energy Metabolism and Angiogenesis in Fetal Calf Muscle

  Susumu Muroya, Yi Zhang, Aoi Kinoshita, Kounosuke Otomaru, Kazunaga Oshima, Yuji Gotoh, Ichiro Oshima, Mitsue Sano, Sanggun Roh, Mika Oe, Koichi Ojima, Takafumi Gotoh

  Metabolites 11 (9) 582 - 582 2021/08/28 [Refereed]
   

  To elucidate the mechanisms underlying maternal undernutrition (MUN)-induced fetal skeletal muscle growth impairment in cattle, the longissimus thoracis muscle of Japanese Black fetal calves at 8.5 months in utero was analyzed by an integrative approach with metabolomics and transcriptomics. The pregnant cows were fed on 60% (low-nutrition, LN) or 120% (high-nutrition, HN) of their overall nutritional requirement during gestation. MUN markedly decreased the bodyweight and muscle weight of the fetus. The levels of amino acids (AAs) and arginine-related metabolites including glutamine, GABA, and putrescine were higher in the LN group than those in the HN group. Metabolite set enrichment analysis revealed that the highly different metabolites were associated with the metabolic pathways of pyrimidine, glutathione, and AAs such as arginine and glutamate, suggesting that MUN resulted in AA accumulation rather than protein accumulation. The mRNA expression levels of energy metabolism-associated genes, such as PRKAA1, ANGPTL4, APLNR, CPT1B, NOS2, NOS3, UCP2, and glycolytic genes were lower in the LN group than in the HN group. The gene ontology/pathway analysis revealed that the downregulated genes in the LN group were associated with glucose metabolism, angiogenesis, HIF-1 signaling, PI3K-Akt signaling, pentose phosphate, and insulin signaling pathways. Thus, MUN altered the levels of AAs and expression of genes associated with energy expenditure, glucose homeostasis, and angiogenesis in the fetal muscle.
• Effects of low and high levels of maternal nutrition consumed for the entirety of gestation on the development of muscle, adipose tissue, bone, and the organs of Wagyu cattle fetuses

  Zhang, Y, Otomaru, K, Oshima, K, Goto, Y, Oshima, I, Muroya, S, Sano, M, Saneshima, R, Nagao, Y, Kinoshita, A, Okamura, Y, Roh, S, Ohtsuka, A, Gotoh, T

  Animal Science Journal 92 (1) e13600  1344-3941 2021/07 [Refereed]
   

  This study aimed to investigate the effects of high and low levels of energy intake during the entire gestation period on the skeletal muscle development, organ development, and adipose tissue accumulation in fetuses of Wagyu (Japanese Black) cows, a breed with highly marbled beef. Cows were allocated to a high-nutrition (n = 6) group (fed 120% of the nutritional requirement) or low-nutrition (n = 6) group (fed 60% of the nutritional requirement). The cows were artificially inseminated with semen from the same sire, and the fetuses were removed by cesarean section at 260 ± 8.3 days of fetal age and slaughtered. The whole-body, total muscle, adipose, and bone masses of the fetal half-carcasses were significantly higher in the high-nutrition group than the low-nutrition group (p = 0.0018, 0.009, 0.0004, and 0.0362, respectively). Fifteen of 20 individual muscles, five of six fat depots, nine of 17 organs, and seven of 12 bones that were investigated had significantly higher masses in the high-nutrition group than the low-nutrition group. The crude components and amino acid composition of the longissimus muscle significantly differed between the low- and high-nutrition groups. These data indicate that maternal nutrition during gestation has a marked effect on the muscle, bone, and adipose tissue development of Wagyu cattle fetuses.
• Effects of early high nutrition related to metabolic imprinting events on growth, carcass characteristics, and meat quality of grass-fed Wagyu (Japanese Black cattle)

  Sithyphone Khounsaknalath, Kotaro Etoh, Kaori Sakuma, Kunihiko Saito, Akira Saito, Tsuyoshi Abe, Fumio Ebara, Toshie Sugiyama, Eiji Kobayashi, Takafumi Gotoh

  Journal of Animal Science 99 (6) 1 - 9 0021-8812 2021/06 [Refereed]
   

  The study was conducted to clarify how early high plane of nutrition related to metabolic imprinting affected growth, carcass characteristics, and meat quality of grass-fed Wagyu (Japanese Black cattle). Wagyu steers were allocated randomly into 2 dietary groups: (1) steers fed milk replacer (crude protein 26.0%, crude fat 25.5%; maximum intake 0.6 kg/d) until 3 mo of age and then fed roughage (orchard grass hay) ad libitum from 4 to 10 mo of age (roughage group, RG; n = 11); (2) steers fed milk replacer (maximum intake of 1.8 kg/d) until 3 mo of age and then fed a high-concentrate diet from 4 to 10 mo of age (early high nutrition, EHN; n = 12). After 11 mo of age, all steers were fed roughage ad libitum until 31 mo of age and then slaughtered. Growth performance, carcass traits, longissimus muscle (LM) meat quality and intramuscular fat (IMF) content, plasma insulin-like growth factor I (IGF-I) concentration, and bone mineral density were measured. Body weight was greater in EHN steers (571 kg) than RG steers (520 kg; P < 0.01). Plasma IGF-I levels were higher in EHN steers than in RG steers at 3, 10, and 14 mo of age (P < 0.01, P < 0.005, P < 0.001, respectively); however, plasma IGF-I levels were lower in EHN steers compared with RG steers at 30 mo of age (P < 0.01). The total weight of the muscles and bones of the left half of the carcass was not different between the 2 groups (P = 0.065). Five of the 19 muscles investigated (semimembranosus, P = 0.036; infraspinatus, P = 0.024; supraspinatus, P = 0.0019; serratus ventralis cervicis, P = 0.032; serratus ventralis thoracis, P = 0.027) were heavier in EHN steers. Total fat weight in the left half of the carcass was 30% greater (P = 0.025) in HNE carcasses. Subcutaneous and perirenal fat weights were 53% and 84% greater (P = 0.008, P = 0.002, respectively) in EHN carcasses. The LM IMF content was greater in EHN loins (13.2%) compared with RG loins (9.4%) at 31 mo of age (P = 0.038); however, no differences were found for shear force, tenderness, and cook loss. These results suggested early high-nutrition affected the growth and meat quality of livestock.
• エピジェネティクスを基盤とした代謝プログラミングの牛肉生産への応用ポテンシャル.

  後藤貴文

  化学と生物 58 280 - 287 2020 [Refereed][Not invited]
  
• Type-I interferon regulates matrix metalloproteinases clearance of the bovine endometrial spheroid.

  Al-Nur Md Iftekhar Rahman, Seiya Yamashita, Md Rashedul Islam, Taisuke Fujihara, Hayato Yamaguchi, Manabu Kawahara, Masashi Takahashi, Hideyuki Takahashi, Takafumi Gotoh, Nobuhiko Yamauchi

  Animal science journal = Nihon chikusan Gakkaiho 91 (1) e13350  2020/01 [Refereed][Not invited]
   

  This study investigated the effect of type-I interferon (IFN) on the expression of matrix metalloproteinases (MMPs) of the bovine endometrial stromal cells (BES) and epithelial cells (BEE). The cells were separated and purified from the caruncles and cultured in DMEM/F-12 containing 10% fetal bovine serum. Spheroids were generated by using ascorbate. Zymograms of the supernatant showed that BEE predominantly expressed MMP-9, whereas MMP-2 was expressed in BES and homo-spheroids. While MMPs expression was not detected in hetero-spheroids. Real-time quantitative PCR revealed that type-I IFN and P4 suppressed the gene expression of MMP-2 and MMP-9 in hetero-spheroids, respectively. On the other hand, gelatin zymography analysis of the supernatant showed that type-I IFN strongly promote the clearance of MMPs. While zymograms of the MMPs stocked in the hetero-spheroids were significantly reduced by type-I IFN. Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP-2 and MMP-9 induced by type-I IFN. Moreover, collagen fibers in hetero-spheroids significantly decreased after the treatment with type-I IFN. In conclusion, it was suggested that type-I IFN participate in the tissue remodeling by regulation the clearance of MMPs.
• Adjusting for Non-response Bias Using Registration Data from an Internet Research Company : Application to Consumers' Evaluation of Japanese Grass-fed Beef

  楠戸 建, 後藤 貴文, 髙橋 義文, 矢部 光保

  統計数理 = Proceedings of the Institute of Statistical Mathematics 情報・システム研究機構統計数理研究所 67 (1) 51 - 62 0912-6112 2019/06 [Refereed][Not invited]
  
• Effect of feeding rice whole crop silage on growth rate, levels of vitamin A, β-carotene, vitamin E and IGF-1 in plasma and skeletal muscle protein degradation in Japanese black calves.

  Katsuhiko Tsuruoka, Takami Kurahara, Hidenobu Kanamaru, Hideyuki Takahashi, Takafumi Gotoh

  Animal science journal = Nihon chikusan Gakkaiho 90 (6) 728 - 736 2019/06 [Refereed][Not invited]
   

  This study evaluated the effects of rice whole crop silage (RWCS) on growth, plasma levels of vitamin A, β-carotene, vitamin E and IGF-1, and expression of genes involved in muscle protein degradation and synthesis in Japanese Black calves. Eleven calves were divided into RWCS (fed RWCS ad libitum and concentrate, n = 5) and control groups (fed hay ad libitum and concentrate, n = 6). Final body weight and dairy gain were significantly larger in the RWCS group compared with the control group. Plasma β-carotene and vitamin E concentrations were significantly higher in the RWCS group compared with control group. Although plasma vitamin E concentration in the RWCS group significantly increased from 4 to 9 months of age, it did not increase in the control group. At 6 months of age in the RWCS group, ubiquitin B (p < 0.05) and calpain 1 (p = 0.097) mRNA expression were lower than control group, but they were not different between groups at 9 months of age. These results indicate that RWCS increases plasma β-carotene level and promotes muscle growth because of a decrease in the rate of protein degradation, but the effect is lost with the increase in plasma vitamin E level.
• Effects of feeding condensed barley distillers soluble on growth rate, ruminal fermentation, plasma metabolites, and myofiber properties of the longissimus thoracis muscle in Japanese Black calves.

  Katsuhiko Tsuruoka, Takami Kurahara, Hidenobu Kanamaru, Hideyuki Takahashi, Takafumi Gotoh

  Animal science journal = Nihon chikusan Gakkaiho 90 (5) 637 - 648 2019/05 [Refereed][Not invited]
   

  This study evaluated the effects of condensed barley distillers soluble (CBDS) on growth rate, rumen fermentation, plasma metabolite, and myofiber properties, and gene expression related to metabolism in the skeletal muscles of Japanese Black calves, compared with soybean meal and corn. Twenty-four calves were divided into four groups: fed 5% CBDS based on the hay dry matter weight (low CBDS) and fed soybean meal and corn at the same nutrition level (control); and fed 15% CBDS based on the hay dry matter weight (high CBDS) and fed soybean meal and corn at the same nutrition level (high soy). The daily gain was larger in the low (p = 0.08) and high (p < 0.05) CBDS groups compared with the control group. In the CBDS-fed groups, plasma β-hydroxybutyric acid concentrations were significantly higher at 6 months of age (p < 0.05), the percentage of type I myofibers was significantly lower and their diameters were significantly larger at 9 months of age (p < 0.05), and carnitine palmitoyltransferase 1b mRNA expression was significantly lower (p < 0.05) and citrate synthase mRNA expression tended to be lower (low; p = 0.06, high; p = 0.05) compared with control group. Thus, feeding CBDS promotes growth and leads to animals with more glycolytic and less oxidative muscle metabolism.
• Correlation between skeletal muscle fiber type and free amino acid levels in Japanese Black steers.

  Daisuke Mashima, Yoshiaki Oka, Takafumi Gotoh, Shozo Tomonaga, Shoko Sawano, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya

  Animal science journal = Nihon chikusan Gakkaiho 90 (4) 604 - 609 2019/04 [Refereed][Not invited]
   

  Free amino acids are important components of tastants and flavor precursors in meat. To clarify the correlation between muscle fiber type and free amino acids, we determined the concentrations of various free amino acids and dipeptides in samples of different muscle tissues (n = 21), collected from 26-month-old Japanese Black steers (n = 3) at 2 days postmortem. The proportions of the myosin heavy chain (MyHC), slow (MyHC1) and fast (MyHC2) isoforms were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The contents of free amino acids and dipeptides were measured by high performance liquid chromatography (HPLC). The MyHC isoform composition varied among the tissue samples. The MyHC1 proportion ranged from 6.9% ± 3.9% to 83.3% ± 16.7%. We confirmed that there was a strong positive correlation between MyHC1 composition and total free amino acid concentrations, including those for two dipeptides. Among the 31 measured free amino acids and dipeptides, 11 showed significant positive correlations and five showed significant negative correlations with MyHC1 composition. These results suggest that a high MyHC1 content induces high free amino acid contents in bovine muscles possibly because of greater oxidative metabolism. This high level of free amino acids could contribute to the intense flavor of meat that is rich in slow-twitch fibers.
• First Identification of the Causal Mutation for Coagulation F11 Deficiency in Hanwoo Cattle

  CHO Sung Hyun, SEO Dongwon, GANBOLD Onolragchaa, CHOI Nu Ri, MANJULA Prabuddha, JIN Shil, LEE Seung Hwan, YAMAGUCHI Nobuhiko, GOTOH Takafumi, LEE Jun Heon

  九州大学大学院農学研究院紀要 Faculty of Agriculture, Kyushu University 64 (1) 55 - 59 0023-6152 2019/02/28 [Refereed][Not invited]
   

  Plasma thromboplastin antecedent (factor XI or F11) deficiency is a relatively mild hemorrhagic genetic disorder reported in Holstein and Japanese black cattle that is caused by two insertional mutations. In this study, we identified a causal mutation of F11 deficiency in Hanwoo cattle and developed a more efficient method to identify this mutation. A total of 2,043 Hanwoo samples from 35 farms, representing three regions (Yeongam, Haman, Yanggu) were used in this study. The mutation is caused by the insertion of 15 bp at exon 9 of the F11 gene; the same mutation has been described in Japanese black cattle. In addition, the gene frequency of F11 deficiency was 1.52% in the Hanwoo cattle population, and all mutant alleles appeared in the heterozygous animals. Especially, this result suggests that 15 bp insertion might be caused by a semi–embryonic lethal gene, which could affect the Hanwoo population. Although, the proportion of the disorder allele is low, the system for verifying this deficiency and additional phenotypic symptoms are needed to prevent any kind of losses in Hanwoo industry. Also the novel TaqMan assay genotyping method for the identification of F11 deficiency proposed in this study offers rapid and accurate results and may be efficiently applied in Hanwoo breeding programs.
• Analysis of differentially expressed genes and the promoters in bovine endometrium throughout estrus cycle and early pregnancy.

  Sayed A A Musavi, Seiya Yamashita, Taisuke Fujihara, Hironori Masaka, Md Rashedul Islam, Sangwan Kim, Takafumi Gotoh, Manabu Kawahara, Kosuke Tashiro, Nobuhiko Yamauchi

  Animal science journal = Nihon chikusan Gakkaiho 89 (11) 1609 - 1621 2018/11 [Refereed][Not invited]
   

  Endometrial gene expression is primarily regulated by the ovarian steroids and pregnancy recognition factors. This study was aimed to characterize differential expression genes (DEGs) in bovine endometrium together with the analysis of their promoter region. Bovine uteri at follicular stage (FS), luteal stage (LS), and implantation stage (IS) at Day 18 of pregnancy were collected. Total RNA extracted and prepared cDNA were then subjected to high-throughput sequencing. For promoter analysis, 1 kb upstream promoter region of each DEG was analyzed. The numbers of highly expressed DEGs were 496 and 597 at FS and LS, respectively. When compared the gene expression of IS with LS, 383 and 346 DEGs showed higher and lower expression at IS, respectively. It was also observed that 20-30 transcription factors (TFs) were included in each DEGs. In addition, promoter analyses estimated 150-160 TFs for each stage. DLX4 and interferon regulatory factor 4 (IRF4) at FS, and IRF5, IRF9, STAT1, and STAT2 at IS were in common to DEGs and estimated TFs, respectively. This study highlighted potential molecular mechanisms controlling endometrial function during estrus cycle and IS, which will further guide to better understand the endometrial functions in future studies.
• Current situation and future prospects for global beef production: overview of special issue.

  Stephen B Smith, Takafumi Gotoh, Paul L Greenwood

  Asian-Australasian journal of animal sciences 31 (7) 927 - 932 2018/07 [Refereed][Not invited]
   

  The demand for beef as a protein source is increasing worldwide, although in most countries beef accounts for considerably less than half of total meat consumption. Beef also provides a highly desirable eating experience in developed countries and, increasingly, in developing countries. The sustainability of beef production has different meanings in the various geographical and socio-economic regions of the world. Natural resources including land mass and uses, rainfall and access to livestock feed, and the robustness of the economy are major determinants of the perception of beef sustainability. In this overview of the 2016 International Symposium on "Future Beef in Asia" and this subsequent Special Edition of the Asian-Australasian Journal of Animal Sciences on "Current Situation and Future Prospects for Global Beef Production", the contributions have been grouped into the following categories: Countries in Southeast Asia; Europe; and Countries producing highly marbled beef for export and/or domestic consumption. They also include reference to Special Topics including marbled beef production, and use of "omics" technologies to enhance beef quality assurance. Among these broad categories, notable differences exist across countries in the production and marketing of beef. These reflect differences in factors including natural resource availability and climate, population size, traditional culture and degree of economic development including industrial and technological developments. We trust that the International Symposium and this Special Edition on Current Situation and Future Prospects for Global Beef Production, the contents of which that are briefly summarized in this paper, will serve as a valuable resource for the livestock industries, researchers and students with an interest in enhancing the prospects for sustainable, efficient beef production that satisfies the growing size and complexity of consumer demands and markets for beef.
• The Japanese Wagyu beef industry: current situation and future prospects - A review.

  Takafumi Gotoh, Takanori Nishimura, Keigo Kuchida, Hideyuki Mannen

  Asian-Australasian journal of animal sciences 31 (7) 933 - 950 2018/07 [Refereed][Not invited]
   

  In Japan, Wagyu cattle include four Japanese breeds; Black, Brown, Shorthorn, and Polled. Today, the renowned brand name Wagyu includes not only cattle produced in Japan, but also cattle produced in countries such as Australia and the United States. In recent years, the intramuscular fat percentage in beef (longissimus muscle) from Japanese Black cattle has increased to be greater than 30%. The Japanese Black breed is genetically predisposed to producing carcass lipids containing higher concentrations of monounsaturated fatty acids than other breeds. However, there are numerous problems with the management of this breed including high production costs, disposal of untreated excrement, the requirement for imported feed, and food security risks resulting from various viral diseases introduced by imported feed. The feeding system needs to shift to one that is more efficient, and improves management for farmers, food security for consumers, and the health environment for residents of Japan. Currently, we are developing a metabolic programming and an information and communications technology (ICT, or Interne of Things) management system for Wagyu beef production as future systems. If successful, we will produce safe, high-quality Wagyu beef using domestic pasture resources while solving the problems of how to utilize increasing areas of abandoned agricultural land and to make use of the plant-based feed resources in Japan's mountainous areas.
• Downregulated angiopoietin-like protein 8 production at calving related to changes in lipid metabolism in dairy cows.

  Misato Nakano, Yutaka Suzuki, Satoshi Haga, Eri Yamauchi, Dahye Kim, Koki Nishihara, Keiichi Nakajima, Takafumi Gotoh, Seungju Park, Myunggi Baik, Kazuo Katoh, Sanggun Roh

  Journal of animal science 96 (7) 2646 - 2658 2018/06/29 [Refereed][Not invited]
   

  Acute physiological adaptation of lipid metabolism during the postpartum transition period of cows facilitates peripheral metabolic regulation. Hepatokines, which are hormones secreted from hepatocytes, are presumed to play a critical role in systemic metabolic regulation. Angiopoietin-like protein 8 (ANGPTL8) has been identified as a novel hepatokine associated with circulating triglyceride concentrations in mice and humans. However, regulation of ANGPTL8 and its physiological effects is still unknown in cattle. The present study aimed to reveal changes in ANGPTL8 expression and secretion during the periparturient period, and to investigate its regulatory effect on adipocytes and mammary epithelial cells. In the peripartum period, liver ANGPTL8 mRNA expression was lesser on the day of parturition and 1 wk postpartum than it was 1 wk before parturition (P < 0.05). Moreover, plasma ANGPTL8 concentrations decreased on the day of parturition as compared with that 1 wk before parturition (P < 0.05). In addition, ANGPTL8 expression in cultured bovine hepatocytes was downregulated after oleate and palmitate treatment but upregulated after insulin treatment (P < 0.05). ANGPTL8 decreased hormone-sensitive lipase (HSL) expression in differentiated adipocytes and cluster of differentiation 36 (CD36), fatty acid synthase (FAS), acetyl-coa carboxylase (ACC), and stearoyl-coa desaturase (SCD) in cultured bovine mammary epithelial cells (P < 0.05). These data suggest that hepatic ANGPTL8 production was downregulated postpartum when the cows experienced a negative energy balance. This downregulation was associated with increased concentrations of NEFA and decreased concentrations of insulin in lactating cows, and it facilitated lipid mobilization from adipose tissue to the mammary glands. We speculate that ANGPTL8 might have beneficial effects in reverting or improving the physiological adaptation and pathological processes of lipid metabolism during the peripartum period.
• Development of Implantable Wireless Sensor Nodes for Animal Husbandry and MedTech Innovation

  Jian Lu, Lan Zhang, Dapeng Zhang, Sohei Matsumoto, Hiroshi Hiroshima, Ryutaro Maeda, Mizuho Sato, Atsushi Toyoda, Takafumi Gotoh, Nobuhiro Ohkohchi

  Sensors 18 (4) 979 - 979 2168-9229 2018/03/26 [Refereed][Not invited]
   

  In this paper, we report the development, evaluation, and application of ultra-small low-power wireless sensor nodes for advancing animal husbandry, as well as for innovation of medical technologies. A radio frequency identification (RFID) chip with hybrid interface and neglectable power consumption was introduced to enable switching of ON/OFF and measurement mode after implantation. A wireless power transmission system with a maximum efficiency of 70% and an access distance of up to 5 cm was developed to allow the sensor node to survive for a duration of several weeks from a few minutes' remote charge. The results of field tests using laboratory mice and a cow indicated the high accuracy of the collected biological data and bio-compatibility of the package. As a result of extensive application of the above technologies, a fully solid wireless pH sensor and a surgical navigation system using artificial magnetic field and a 3D MEMS magnetic sensor are introduced in this paper, and the preliminary experimental results are presented and discussed.
• 【よい子牛は母牛の管理から】母牛への栄養・免疫刺激と胎子の免疫・代謝異常 胎子期からの代謝プログラミングの可能性

  後藤 貴文

  臨床獣医 (株)緑書房 36 (1) 12 - 17 0912-1501 2018/01 [Refereed][Not invited]
  
• Development of wireless sensor nodes for animals husbandry and medical applications

  J. Lu, L. Zhang, S. Matsumoto, H. Hiroshima, R. Maeda, T. Gotoh, M. Sato, A. Toyoda, N. Ohkohchi

  Proceedings of IEEE Sensors 2017- 1 - 3 2168-9229 2017/12/21 [Refereed][Not invited]
   

  In this paper, we report the development of ultrasmall and low power wireless sensor nodes and the implantation in animals for food safety as well as for medical innovation. A passive RFID with hybrid interface and neglectable power consumption was introduced for the first time to enable switch ON/OFF and change of measurement modes after implantation. Besides low power consumption, a wireless power transmission module with high efficiency and maximum distance of 5 cm was developed to survive the sensor node for the duration of weeks by a few minutes remote charging. Field test results by using white-mouse and cow indicated high accuracy of the collected biological information and bio-compatibility of the package. Moreover, a surgical navigation system using wireless sensor nodes is proposed and discussed as a potential future application, and the preliminary results is presented herein.
• ICT Contributed to Labor Saving and Efficiency of Beef Production Utilized Abandoned Agricultural Land in Mountainous Area in Japan

  後藤 貴文

  電子情報通信学会誌 = The journal of the Institute of Electronics, Information and Communication Engineers 電子情報通信学会 100 (11) 1242 - 1247 0913-5693 2017/11 [Refereed][Not invited]
  
• Abundance of beige and brown adipocyte markers in different adipose depots of cattle at 26 months of age

  K. Komolka, E. Albrecht, T. Gotoh, S. Maak

  Advances in Animal Biosciences 8 (s1) s38 - s41 2040-4700 2017/10 [Refereed][Not invited]
   

  Activity of brown and beige adipocytes could contribute to breed differences in fat deposition. Therefore, we compared the abundance of markers for adipocyte types in steers from three cattle breeds differing in fat deposition (Japanese Black, Holstein, Charolais). Markers for white (leptin (LEP)), beige (transmembrane protein 26 (TMEM26), uncoupling protein 1 (UCP1)), and brown adipocytes (Zic family member 1 (ZIC1), UCP1) were analysed by quantitative reverse transcription PCR in subcutaneous fat (SCF), intramuscular fat (IMF), intermuscular fat (IRMF), perirenal fat (PF) and visceral fat (VF). LEP messenger RNA (mRNA) was less abundant in VF compared with other depots (P<0.05). TMEM26 was weakly but evenly expressed in all depots in all animals, whereas UCP1 mRNA showed higher individual variation in some depots. ZIC1 was not detectable in VF and PF but abundant in SCF, IMF and even more abundant in IRMF (P<0.05). No significant breed differences were detected. Using antibodies against UCP1, TMEM26 and ZIC1, we demonstrated that fat depots of 26-month-old cattle still comprise different adipocyte types. However, our results did not support the hypothesis that higher energy expenditure associated with higher abundance or activity of beige or brown adipocytes contributed to differences in fat deposition.
• Epigallocatechin-3-gallate increases autophagy signaling in resting and unloaded plantaris muscles but selectively suppresses autophagy protein abundance in reloaded muscles of aged rats

  Hideyuki Takahashi, Yutaka Suzuki, Junaith S. Mohamed, Takafumi Gotoh, Suzette L. Pereira, Stephen E. Alway

  EXPERIMENTAL GERONTOLOGY 92 56 - 66 2017/06 [Refereed][Not invited]
   

  We have previously found that Epigallocatechin-3-gallate (EGCg), an abundant catechin in green tea, reduced apoptotic signaling and improved muscle recovery in response to reloading after hindlimb suspension (HS). In this study, we investigated if EGCg altered autophagy signaling in skeletal muscle of old rats in response to HS or reloading after HS. Fischer 344 x Brown Norway inbred rats (age 34 months) were given 1 ml/day of purified EGCg (50 mg/kg body weight), or the same sample volume of the vehicle by gavage. One group of animals received HS for 14 days and the second group of rats received 14 days of HS, then the HS was removed and they were allowed to recover by ambulating normally around the cage for two weeks. EGCg decreased a small number of autophagy genes in control muscles, but it increased the expression of other autophagy genes (e.g., ATG16L2, SNCA, TM9SF1, Pink1, PIM-2) and HS did not attenuate these increases. HS increased Beclinl, ATG7 and LC3-II/I protein abundance in hindlimb muscles. Relative to vehicle treatment, EGCg treatment had greater ATG12 protein abundance (35.8%, P < 0.05), but decreased Beclinl protein levels (-101.1%, P < 0.05) after HS. However, in reloaded muscles, EGCg suppressed Beclinl and LC3-II/I protein abundance as compared to vehicle treated muscles. EGCg appeared to "prime" autophagy signaling before and enhance autophagy gene expression and protein levels during unloading in muscles of aged rats, perhaps to improve the clearance of damaged organelles. However, EGCg suppressed autophagy signaling after reloading, potentially to increase the recovery of hindlimb muscles mass and function after loading is restored. (C) 2017 Elsevier Inc. All rights reserved.
• TRIENNIAL GROWTH AND DEVELOPMENT SYMPOSIUM: Factors influencing bovine intramuscular adipose tissue development and cellularity.

  E Albrecht, L Schering, Y Liu, K Komolka, C Kühn, K Wimmers, T Gotoh, S Maak

  Journal of animal science 95 (5) 2244 - 2254 2017/05 [Refereed][Not invited]
   

  Appearance, distribution, and amount of intramuscular fat (IMF), often referred to as marbling, are highly variable and depend on environmental and genetic factors. On the molecular level, the concerted action of several drivers, including hormones, receptors, transcription factors, etc., determines where clusters of adipocytes arise. Therefore, the aim of future studies remains to identify such factors as biological markers of IMF to increase the ability to identify animals that deposit IMF early in age to increase efficiency of high-quality meat production. In an attempt to unravel the cellular development of marbling, we investigated the abundance of markers for adipogenic differentiation during fattening of cattle and the transcriptome of muscle and dissected IMF. Markers of different stages of adipogenic differentiation are well known from cell culture experiments. They are usually transiently expressed, such as delta-like homolog 1 (DLK1) that is abundant in preadipocytes and absent during differentiation to mature adipocytes. It is even a greater challenge to detect those markers in live animals. Within skeletal muscles, hyperplasia and hypertrophy of adipocytes can be observed throughout life. Therefore, development of marbling requires, on the cellular level, recruitment, proliferation, and differentiation of adipogenic cells to store excess energy in the form of lipids in new cells. In a recent study, we investigated the localization and abundance of early markers of adipogenic differentiation, such as DLK1, in bovine muscle tissue. An inverse relationship between IMF content and number of DLK1-positive cells in bovine muscle was demonstrated. Considering the cellular environment of differentiating adipocytes in muscle and the secretory action of adipocytes and myocytes, it becomes obvious that cross talk between cells via adipokines and myokines may be important for IMF development. Secreted proteins can act on other cells, inhibiting or stimulating their function via autocrine and paracrine actions. Such factors with potential influence on IMF, among them, agouti signaling protein and thrombospondin 4, were identified in transcriptome analyses and further investigated. Furthermore, results from transcriptome analysis indicate involvement of genes that are not directly related to adipogenesis and lipid metabolism, providing new candidates for future research.
• Preliminary study of the effects of condensed barley distillers soluble on rumen fermentation and plasma metabolites in Japanese Black cows.

  Katsuhiko Tsuruoka, Hidenobu Kanamaru, Hideyuki Takahashi, Takafumi Gotoh

  Animal science journal = Nihon chikusan Gakkaiho 88 (4) 610 - 617 2017/04 [Refereed][Not invited]
   

  In Japan, condensed barley distillers soluble (CBDS) is a widely known liquor byproduct that contains a high level of protein and is used as a supplementary protein feed for cattle. The present study evaluated the effects of CBDS feed on rumen fermentation and plasma metabolites in Japanese Black cows. Applying a replicated 3 × 3 Latin square design, nine cows were offered CBDS and hay (CBDS-t), soy bean meal and hay (Soybean-t) and only hay (Hay-t) over 35 days. We collected ruminal fluid and plasma just before feeding and at 3 h after feeding. The concentrations of propionate and butyrate in the rumen before feeding were lower in the CBDS-t than in the Soybean-t group (P < 0.05). However, after 3 h, the concentrations were higher in the CBDS-t than in the Soybean-t and Hay-t groups (P < 0.05). Although, there were no differences in the compositions (% mol) of propionate and butyrate in the rumen and the concentration of plasma β-hydroxybutyric acid before feeding between treatments, after 3 h they were significantly higher in the CBDS-t than in the Soybean-t and Hay-t groups (P < 0.05). These results indicate that feeding CBDS promotes rumen fermentation and butyrate metabolism.
• Effects of nutritional status on hormone concentrations of the somatotropin axis and metabolites in plasma and colostrum of Japanese Black cows.

  Ouanh Phomvisith, Hideyuki Takahashi, Ha Thi Mai, Yuji Shiotsuka, Atsuko Matsubara, Toshihisa Sugino, Christopher D Mcmahon, Tetsuji Etoh, Ryoichi Fujino, Mitsuhiro Furuse, Takafumi Gotoh

  Animal science journal = Nihon chikusan Gakkaiho 88 (4) 643 - 652 2017/04 [Refereed][Not invited]
   

  We aimed to determine the effects of nutritional status on concentrations of somatotropic axis hormones (growth hormone (GH) and insulin-like growth factor 1 (IGF-1)), insulin and metabolites (glucose, total protein and nonesterified fatty acids (NEFA)) in the plasma and colostrum in late antepartum cows. Eight pregnant Japanese Black cows were randomly assigned to two experimental groups (n = 4 per group). Control cows (CON) received 100% of their nutritional requirements until parturition, whereas restricted group cows (RES) received 60% of their nutritional requirements. Blood samples were taken during the antepartum period, and blood and colostrum samples were collected on days 0, 1, and 3 after calving. Compared to the CON group, the RES group had higher concentrations of GH and NEFA in plasma, but significantly lower concentrations of glucose and insulin in plasma. The concentrations of GH in plasma after calving were significantly higher, but total plasma protein was significantly lower in RES than in CON cows. Compared to the CON group, the RES group had significantly higher concentrations of GH in colostrum, but significantly lower total concentrations of protein in colostrum. Concentrations of IGF-1 were not different between the two groups. These findings suggest that maternal nutritional status during late gestation influences concentrations of GH and total protein in the blood and colostrum of Japanese Black cows.
• Effect of Pen Floor Material on the Behavior of Hanwoo Cattle

  Ka Young Yang, Kyu-Hyun Park, Jae Jung Ha, Takafumi Gotoh, Young Han Song

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 62 (1) 99 - 103 0023-6152 2017/02 [Not refereed][Not invited]
   

  This study was conducted to investigate the effect of three types of pen floor litter materials (sawdust, wood shavings and rice husk) on the behavior of growing Hanwoo calves. Twelve Hanwoo calves (121.4 +/- 19.9 kg) were housed in an open barn for 6 months. Two heifers and two steers were housed in a pen on each litter treatment and two replications per each treatment were conducted. Temperature, humidity and cow behavior were observed after a 15 day adaptation period. Average daily gain was higher in winter and spring than in summer (p<0.05). Feeding behavior was significantly shorter in summer compared with other seasons (p<0.05). Lying behavior was significantly longer in cows housed on the sawdust treatment during summer (p<0.05). The frequency of self licking and social licking behavior was higher in the spring (p<0.05) compared with other seasons. During winter, body and tail rubbing behaviors increased (p<0.05). With consideration of cattle welfare and farm budget, sawdust or wood shavings litter during spring and summer and rice husk litter during winter were regarded as the most beneficial, for optimum lying and licking behavior of Hanwoo beef cattle. Rice husk is usually cheaper during harvest compared with other seasons because of its abundance.
• Miniaturization and packaging of implantable wireless sensor nodes for animals monitoring

  J. Lu, L. Zhang, S. Matsumoto, H. Hiroshima, K. Serizawa, M. Hayase, T. Gotoh

  Proceedings of IEEE Sensors 2168-9229 2017/01/05 [Refereed][Not invited]
   

  This paper reports the development and evaluation of a ultra-small and ultra-light wireless sensor node for real time monitoring of biological information of animals. Power consumption of the sensor node was decreased dramatically by improving efficiency of the MCU and irradiation power of the RFIC, which enables a few months life time when using SR416SW batteries. Various configurations of the antenna were investigated for the pursuit of small package size, while maintain an enough communication distance of a few meters. The implant experiments were carried out to investigate performances of the sensor node as well as practicability of the package. Moreover, the feasibility study of a wireless power transmission module as the power source was performed and discussed herein.
• Translocation of Tektin 3 to the equatorial segment of heads in bull spermatozoa exposed to dibutyryl cAMP and calyculin A.

  Mariko Tsukamoto, Erina Hiyama, Karen Hirotani, Takafumi Gotoh, Tetsuichiro Inai, Hiroshi Iida

  Molecular reproduction and development 84 (1) 30 - 43 2017/01 [Refereed][Not invited]
   

  Tektins (TEKTs) are filamentous proteins associated with microtubules in cilia, flagella, basal bodies, and centrioles. Five TEKTs (TEKT1, -2, -3, -4, and -5) have been identified as components of mammalian sperm flagella. We previously reported that TKET1 and -3 are also present in the heads of rodent spermatozoa. The present study clearly demonstrates that TEKT2 is present at the acrosome cap whereas TEKT3 resides just beneath the plasma membrane of the post-acrosomal region of sperm heads in unactivated bull spermatozoa, and builds on the distributional differences of TEKT1, -2, and -3 on sperm heads. We also discovered that hyperactivation of bull spermatozoa by cell-permeable cAMP and calyculin A, a protein phosphatase inhibitor, promoted translocation of TEKT3 from the post-acrosomal region to the equatorial segment in sperm heads, and that TEKT3 accumulated at the equatorial segment is lost upon acrosome reaction. Thus, translocation of TEKT3 to the equatorial segment may be a capacitation- or hyperactivation-associated phenomenon in bull spermatozoa. Mol. Reprod. Dev. 84: 30-43, 2017. © 2016 Wiley Periodicals, Inc.
• Effects of a high milk intake during the pre-weaning period on nutrient metabolism and growth rate in Japanese Black cattle.

  Atsuko Matsubara, Hideyuki Takahashi, Akira Saito, Aoi Nomura, Khounsaknalath Sithyphone, Christopher D Mcmahon, Ryoichi Fujino, Yuji Shiotsuka, Tetsuji Etoh, Mitsuhiro Furuse, Takafumi Gotoh

  Animal science journal = Nihon chikusan Gakkaiho 87 (9) 1130 - 6 2016/09 [Refereed][Not invited]
   

  This study aimed to determine the effects of feeding an increased volume of high-fat milk during the early post-natal life on metabolite concentrations in the blood, the expression of key genes regulating intermediary metabolism in the skeletal muscles, and the rate of growth of Japanese Black cattle. All calves were fed a high-fat milk replacer (crude protein, 26%; crude fat, 25.5%; total dissolved nitrogen, 116%). Control calves (n = 4) were nursed with 500 g milk replacer until 3 months of age, whereas calves in the experimental group (n = 4) were nursed with 1800 g milk replacer until 3 months, and then the volume was gradually reduced until 5 months. Body weight was significantly higher in the experimental group than in the control group at 7 months. Plasma glucose concentrations were significantly lower in the experimental group. Expression of glucose-transporter-4 messenger RNA (mRNA) was lower, whereas that of glucose transporter 1, cluster of differentiation 36, and carnitine palmitoyltransferase-1b mRNA was significantly higher in the Longissimus thoracis of the experimental group. Nutritional status during early post-natal life appears to strongly influence the growth rate and glucose and lipid metabolism in Japanese Black cattle.
• エピゲノムの食料生産への活用 代謝プログラミングのウシ産肉制御機構のメカニズム解明と応用

  後藤 貴文

  BIO Clinica (株)北隆館 31 (5) 532 - 540 0919-8237 2016/05 [Refereed][Not invited]
   

  胎児期から生後の初期成長期の各臓器の形成・成熟の感受性の高い、いわゆる可塑性の高い時期の栄養環境により、その後の動物体の代謝システムに多大な影響を及ぼす。それはエピジェネティクス研究と関連して代謝プログラミングとも呼ばれる。著者らは、このメカニズムを家畜の飼養技術としてポジティブに取り入れ、初期成長期の栄養制御により、ウシのフルライフにわたる基盤的な代謝レベルをプログラミングし、最終的な産肉性、特に肉質と肉量を制御し、効率的、省力的かつ種々の環境に適合できる次世代型の革新的家畜飼養システムの開発に挑戦している。(著者抄録)
• Transcriptome profiling of Musculus longissimus dorsi in two cattle breeds with different intramuscular fat deposition.

  Elke Albrecht, Katrin Komolka, Siriluck Ponsuksili, Takafumi Gotoh, Klaus Wimmers, Steffen Maak

  Genomics data 7 109 - 11 2016/03 [Refereed][Not invited]
   

  Intramuscular fat (IMF) deposition is a physiological process in cattle and is highly variable among breeds suggesting a large influence of genetic factors besides environmental factors. In order to elucidate molecular pathways underlying the genetic variation in this trait we compared transcriptomes of Musculus longissimus dorsi (MLD) in steers of Japanese Black and Holstein Friesian cattle breeds fed a high energy diet typically applied in Japan to achieve maximum IMF content. We identified a total of 569 differentially expressed genes (DEGs) with the majority (433) up-regulated in Japanese Black cattle. This breed is characterized by an extreme capacity for IMF deposition. Subsequent Ingenuity Pathway Analysis (IPA) revealed a gene network linking parameters of cell morphology and maintenance with lipid metabolism. The data from this study were deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE75348. We provide here a dataset which is of potential value to dissect molecular pathways influencing differences in fat deposition under high-energy nutrition.
• Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system.

  Tatsunori Masatani, Shunpei Yoshihara, Atsuko Matsubara, Takafumi Gotoh, Hideyuki Takahashi, Tetsuya Tanaka, Masako Andoh, Yasuyuki Endo, Tomohide Matsuo

  Acta parasitologica 61 (2) 419 - 24 2016/03 [Refereed][Not invited]
   

  Theirelia orientalis is a tick-borne haemoprotozoan parasite, and infection with this parasite is one of the most important diseases for grazing cattle. Co-infection of cattle with different genotypes of T. orientalis often occurs. In this study, we investigated the temporal dynamics of genotypes in cattle in a year-round grazing system in Japan. Genotype-specific PCR assays to determine major piroplasm surface protein (MPSP) genotypes (types 1 to 5) of T. orientalis were performed by using time-course blood samples collected from grazing cattle and ticks in a pasture. All 20 cattle investigated in this study were infected with T. orientalis. By using genotype-specific PCR, we detected the combination of genotypes of T. orientalis (types 1 to 5) from each cattle. These multiple genotypes of T. orientalis were also confirmed in ticks. Notably, each genotype of T. orientalis in cattle was temporally detected from cattle and more variable genotypes were found in summer. The observed temporal dynamics of the MPSP genotypes of T. orientalis in cattle could be explained by host immunity against the parasites or genetic recombination of parasite in ticks.
• Association of SNPs in AMY1A and AMY2A genes with chicken meat quality and clinical -chemical traits in chicken

  Dongwon Seo, Hee-Bok Park, Nuri Choi, Shil Jin, Kang-Nyeong Heo, Cheorun Jo, Takafumi Gotoh, Jun Heon Lee

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 61 (1) 121 - 125 0023-6152 2016/02 [Refereed][Not invited]
   

  QTL studies can provide useful information with which to identify positional candidate genes and DNA sequence variations that can be used to develop genetic markers. In particular, the amylase in chicken serum may affect meat quality traits that are associated with improved starch digestion, which can be used as an indicator of the health status of a chicken. In this study, we investigated two amylase genes, AMY1A and AMY2A, because these genes are located in the QTL region affecting serum amylase levels. Association analyses based on a mixed linear model were performed to identify relationships between the SNPs present in these two genes and clinical chemical and meat quality traits. As a result, we confirmed that these genes were not significantly associated with any clinical chemical traits, indicating that these two genes are not directly involved in the amylase QTL effect. However, the AMY1A gene is significantly associated with the degree of yellow coloration (b*) in the thigh muscle, and the AMY2A gene is significantly associated with the water holding capacity (WHC) of breast muscle. With further verifications, these genes could be used to improve the quality of these traits in chickens.
• Improvement of Porcine SCNT Embryo Development Using Histone Deacetylase Inhibitors

  Jin-Hee Lee, Ju Lan Chun, Ji Hye Lee, Keun Jung Kim, Eun Young Kim, Kil-Woo Han, Takafumi Gotoh, Min Kyu Kim

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 61 (1) 115 - 120 0023-6152 2016/02 [Refereed][Not invited]
   

  Somatic cell nuclear transfer (SCNT) has been used in crucial and potential fields of the animal reproduction. Despite the successful birth of cloned animals, the cloning efficiency of SCNT has been still low. Abnormal epigenetic reprogramming has been reported as the cause of the low efficiency of SCNT in cloned embryos. Here, the study aimed to improve the developmental competence of porcine SCNT embryos using Trichostatin A (TSA) and Scriptaid which are well known as histone deacetylase inhibitors that would enhance epigenetic reprograming in cloned embryos by suppressing the event of histone deacetylation. Moreover, this study tested whether Scriptaid would be a substitute for TSA because it has been suggested that TSA is involved in malformation of cloned embryos. Various concentrations of Scriptaid were tested and 500 nM Scriptaid treatment resulted in a significant improvement of the cloned embryo during development regarding the blastocyst formation rates. When cloned embryos were treated with 50 nM TSA or/and 500 nM Scriptaid for 15 h or 24 h, the blastocyst rates of reconstructed embryos were increased in comparison to the untreated control group. However, there was no dose dependent difference among groups. When donor cells were treated with 50 nM TSA or/and 500 nM Scriptaid for 4 h the blastocyst rates of reconstructed embryos were increased in comparison with the untreated control group. Moreover the expression levels of histone deacetylase 1 (HDAC1) and histone deacetylase 2 (HDAC2) were decreased with TSA and/or Scriptaid treatment. In conclusion, TSA and/or Scriptaid treatments significantly increased the developmental competence of porcine SCNT embryos. In addition, Scriptaid improved the development of SCNT embryos regardless of the TSA treatment. Therefore, Scriptaid would be an alternative additive to improve the development competence of cloned embryos after SCNT.
• Association of HSPB1 and CRYAB SNPs with Chicken Meat Quality and Robustness in Five Lines of Korean Native Chicken

  Dongwon Seo, Hee-Bok Park, Samooel Jung, Prabuddha Manjula, Nuri Choi, Shil Jin, Kang-Nyeong Heo, Cheorun Jo, Takafumi Gotoh, Jun Heon Lee

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 61 (1) 127 - 132 0023-6152 2016/02 [Refereed][Not invited]
   

  The clinical chemical traits of blood serum are important biomarkers that can be used to investigate health and metabolic status, both of which can affect meat quality traits. In this study, the SNPs in the genes HSPB1 (g. 526A > G; 5'UTR) and CRYAB (g. 2471T > C; exon 2) were investigated via an association analysis with meat quality and clinical chemical traits. In total, 597 native Korean chickens were included in the study. There were no significant associations between the SNP in CRYAB and either meat quality or serum traits. On the other hand, the SNP in HSPB1 was significantly associated (P<0.05) with water holding capacity (WHC), lightness (L*), and yellowness (b*) of the thigh muscle. In addition, two clinical chemical traits, high density lipoprotein (HDL-c) and amylase (Amy), were significantly associated with this SNP. Of these, the WHC, L*, and b* values of the thigh muscle have the dominant effects only, while Amy trait exhibits both additive and dominant effects. These results can provide useful information that can facilitate the improvement of chicken meat quality, and the understanding of the relationship between blood metabolism and meat quality.
• Effects of Dietary Zilpaterol Hydrochloride on Growth Performance, Carcass Characteristics, and Serum Compositions of Hanwoo Bulls

  Jong Kyoo Kim, Ki Yong Chung, Hyeong Cheol Kim, Eun Mi Lee, Sun Sik Chang, Eung Gi Kwon, Bradley J. Johnson, Takafumi Gotoh

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 61 (1) 109 - 114 0023-6152 2016/02 [Refereed][Not invited]
   

  This study was conducted to determine the effects of zilpaterol hydrochloride (ZH) on the growth performance and carcass characteristics of Hanwoo bulls. Twenty Hanwoo bulls (653 +/- 22.1) were randomly assigned by body weight (BW) to determine the effects of feeding zilpaterol hydrochloride (Ten bulls with 8.3 mg/kg ZH and ten bulls without ZH) on the ultrasound measurement, carcass traits, and serum compositions. Treatments comprised diets with and without ZH supplemented for 20 days prior to harvest with three days withdrawal period. Treatment of ZH led to an increase either in the final BW or average daily gain (ADG) in Hanwoo bulls (P<0.05). There was no treatment effect on the back fat thickness, ribeye area, and marbling score (P>0.05). ZH treatment tended to increase at dressing percentage (P=0.068). There was a high positive correlation (R-2=0.9226) at treatment but relatively low positive correlation (R-2=0.7156) at control between ultrasound longissimus muscle area and ribeye area. The treatment of ZH affects the serum triglyceride and non esterified fatty acid (P<0.05). Moreover, we demonstrated that the treatment of ZH did not have a negative effect on the serum level of GOT or GPT of Hanwoo bulls. We concluded that the treatment of ZH has little impact on the carcass trait of Hanwoo bulls regardless of the positive effect on BW and ADG. However, dietary ZH increased lipid related serum metabolites of Hanwoo bulls at 14th day.
• 暑熱ストレス下の仔牛における日増体量、血液代謝産物およびストレスホルモン濃度に及ぼすBacillus subtilis C-3102株給与の影響.

  髙橋秀之, 浦川めぐみ, 渡邉一史, 芦田延久, 今林寛和, 佐藤光美, 佐藤富雄, 渡邊康一, 大和田修一, 後藤貴文, 麻生久

  東北畜産学会報 65 (3) 54 - 56 2016 [Refereed][Not invited]
  
• Higher Intake of Milk-Replacer Pre-Weaning Enhances Post-Weaning Insulin-Like Growth Factor 1 Levels in Japanese Black Cattle

  Hideyuki Takahashi, Atsuko Matsubara, Akira Saito, Ouanh Phomvisith, Akari Shiga, Ha T Mai, Toshihisa Sugino, Christopher D McMahon, Tetsuji Etoh, Yuji Shiotsuka, Ryoichi Fujino, Mitsuhiro Furuse, Takafumi Gotoh

  Journal of Veterinary Science & Technology 8 (1) 409  2016 [Refereed][Not invited]
  
• Physiological Roles of Adipokines, Hepatokines, and Myokines in Ruminants.

  Sang-Gun Roh, Yutaka Suzuki, Takafumi Gotoh, Ryuichi Tatsumi, Kazuo Katoh

  Asian-Australasian journal of animal sciences 29 (1) 1 - 15 2016/01 [Refereed][Not invited]
   

  Since the discovery of leptin secreted from adipocytes, specialized tissues and cells have been found that secrete the several peptides (or cytokines) that are characterized to negatively and positively regulate the metabolic process. Different types of adipokines, hepatokines, and myokines, which act as cytokines, are secreted from adipose, liver, and muscle tissue, respectively, and have been identified and examined for their physiological roles in humans and disease in animal models. Recently, various studies of these cytokines have been conducted in ruminants, including dairy cattle, beef cattle, sheep, and goat. Interestingly, a few cytokines from these tissues in ruminants play an important role in the post-parturition, lactation, and fattening (marbling) periods. Thus, understanding these hormones is important for improving nutritional management in dairy cows and beef cattle. However, to our knowledge, there have been no reviews of the characteristics of these cytokines in beef and dairy products in ruminants. In particular, lipid and glucose metabolism in adipose tissue, liver tissue, and muscle tissue are very important for energy storage, production, and synthesis, which are regulated by these cytokines in ruminant production. In this review, we summarize the physiological roles of adipokines, hepatokines, and myokines in ruminants. This discussion provides a foundation for understanding the role of cytokines in animal production of ruminants.
• Characteristics and Health Benefit of Highly Marbled Wagyu and Hanwoo Beef.

  Takafumi Gotoh, Seon-Tea Joo

  Korean journal for food science of animal resources 36 (6) 709 - 718 2016 [Refereed][Not invited]
   

  This review addresses the characteristics and health benefit of highly marbled Wagyu and Hanwoo beef. Marbling of Wagyu and Hanwoo beef has been increased in Japan and Korea to meet domestic consumer preferences. Wagyu and Hanwoo cattle have high potential of accumulating intramuscular fat (IMF) and producing highly marbled beef. The IMF content varies depending on the feeding of time, finishing diet, and breed type. IMF increases when feeding time is increased. The rate of IMF increase in grain-fed cattle is faster than that in pasture-fed cattle. Fatty acid composition are also different depending on breeds. Highly marbled Wagyu and Hanwoo beef have higher proportions of monounsaturated fatty acid (MUFA) due to higher concentrations of oleic acid. MUFAs have little effect on total cholesterol. They are heart-healthy dietary fat because they can lower low-density lipoprotein (LDL)-cholesterol while increasing high-density lipoprotein (HDL)-cholesterol. Clinical trials have indicated that highly marbled beef does not increase LDL-cholesterol. This review also emphasizes that high oleic acid beef such as Wagyu and Hanwoo beef might be able to reduce risk factors for cardiovascular disease.
• Effects of Trehalose and Sucrose on DNA Integrity of Evaporatively Dried Boar Spermatozoa and Embryo Development After ICSI

  Xiao Xia Li, Young Joo Yi, Ji Hey Lee, Keun Jung Kim, Eun Young Kim, Lili Zhuang, Ju Lan Chun, Kyung-Bon Lee, Takafumi Gotoh, Min Kyu Kim

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 60 (2) 385 - 392 0023-6152 2015/09 [Refereed][Not invited]
   

  Evaporative-drying, a novel technique for sperm preservation, has many advantages however, the results were inferior to freeze drying. The objective of this study is to investigate the effects of trehalose or sucrose supplemented into desiccation/preservation solution for sperm dehydration on sperm DNA integrity and the development of porcine embryos after intracytoplasmic injected with evaporatively dried boar spermatozoa. The addition of 0.2 M trehalose or sucrose significantly reduced DNA damage of sperm compared to those of other concentrations. The rate of cleaved embryo and blastocyst formation was significantly increased in oocytes injected with evaporatively dried sperm with 0.2 M trehalose. According to TUNEL assay, total cell number was increased, and the indices of TUNEL, fragmentation and total apoptosis were significantly decreased in blastocysts from ICSI with sperm treated with trehalose. Also, evaporatively dried sperm with trehalsoe were preserved at 4 degrees C for 1 year, and successfully produced pre implantation embryos after ICSI that transferred to the recipients became pregnant and maintained pregnancy periods until 3 months. Consequently, the present study found out that trehalose could reduce DNA damage of sperm during dehydration, and oocytes injected with sperm treated with trehalose developed to pre implantation embryos that might produce normal piglets after embryo transfer (ET).
• Determination of Intramuscular Fat Content in Beef using Magnetic Resonance Imaging

  Sangdae Lee, Santosh Lohumi, Hyoun-Sub Lim, Takafumi Gotoh, Byoung-Kwan Cho, Samooel Jung

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 60 (1) 157 - 162 0023-6152 2015/02 [Refereed][Not invited]
   

  The amount and distribution of intramuscular fat in beef and pork are some of the most important quality characteristics because they highly influence the nutritional value and eating quality of the meat. Magnetic resonance imaging (MRI) is a suitable non invasive alternative method that can provide both qualitative and quantitative information on beef meats with high spatial information. In this study, we used MRI technique in combination with image processing tools for the visualization and prediction of-intramuscular fat distribution in beef meats. The results from image analysis were compared with the chemical reference result for the percentage of intramuscular fat. The results show a strong correlation (R-2 = 0.98) between the MRI detected and chemically measured values. The distribution uniformity of intramuscular fat within the beef meats was evaluated by calculating their distribution and pixel size in the MRI according to four levels of beef quality grade (1+, 1,2, and 3).
• Identification of Polymorphisms in Plumage Color Related Genes in Korean Native Ducks

  Hasina Sultana, Dong-Won Seo, Hee-Bok Park, Muhammad Cahyadi, Shil Jin, Md. -Rashedul Hoque, Yeon-Su Kim, Kang-Nyeong Heo, Cheorun Jo, Takafumi Gotoh, Jun-Heon Lee

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 60 (1) 119 - 126 0023-6152 2015/02 [Refereed][Not invited]
   

  Identification of genetic polymorphisms in the genes related to plumage color and elucidation of their associations with plumage phenotypes are important for the selection of desirable plumage colors. The aim of this study is to identify candidate genetic polymorphisms conferring white plumage color to select ducks with desirable plumage colors. Single nucleotide polymorphism (SNPs) in 4 candidate genes, namely the Agouti signaling protein gene (ASIP), Dopachrome tautomerase gene (DCT), Melanocortin 1 receptor gene (MC1R), and Microphthalmia-associated transcription factor gene (MITF), were investigated in forty birds, of which 20 were colored Korean native ducks and 20 were white-colored commercial ducks. Twenty-one primer pairs were designed to amplify entire exons along with partial introns of the 4 genes, and the PCR products were sequenced. A total of 83 polymorphisms were identified in these genes. We identified 8 and 36 SNPs in the ASIP and MITF genes, respectively. Thirty polymorphisms, comprising of 4 non-synonymous SNPs, were identified in the DOT gene. Two non-synonymous SNPs were identified in the MC1R gene. Two genetic variations [(c.7260>T and g.10585-88->AATC (4-bp indel)] in the DOT gene revealed significant associations with the 2 different colored breeds (P<0.001). The results of this study, after further verifications of the candidate SNPs, can be helpful for the selection of Korean native ducks with desirable plumage colors.
• Association of Variation in the MC4R Gene with Meat Quality Traits in a Commercial Pig Population

  Kang Kwon, Muhammad Cahyadi, Hee-Bok Park, Dong Won Seo, Shil Jin, Sang-Wook Kim, Yang-Il Choi, Kwan Suk Kim, Takafumi Gotoh, Jun Heon Lee

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 60 (1) 113 - 118 0023-6152 2015/02 [Refereed][Not invited]
   

  The melanocortin-4 receptor (MC4R) gene is known to encode a membrane bound receptor protein and is a member of the melanocortin receptor family of genes. In mammals, these genes are involved in energy homeostasis and in regulating feeding behavior and body weight. The objective of the present study was to examine if there were any associations between variations in the MC4R gene with meat quality traits in a commercial pig population in Korea. Among the total of 593 commercial pigs, sire information was retrieved from 232 pigs. These animals were successfully genotyped for the c.892A>G (p.Asp298Asn) single nucleotide polymorphism (SNP) by using TaqI PCR RFLP methods. Association analyses between this SNP and meat quality traits were performed using a general linear model (GLM) including sire effect. This SNP was significantly associated with backfat thickness (P<0.05), marbling (P<0.01). Interestingly, this SNP marker was also associated with volatile basic nitrogen after 14 days of storage (P<0.05). To our knowledge, it is the first results observed for the MC4R genotypes with volatile basic nitrogen after 14 days of storage in the commercial pig population. Therefore, these results suggest that the MC4R gene can be targeted in marker assisted breeding for selecting pigs with good meat quality.
• Localization and abundance of early markers of fat cell differentiation in the skeletal muscle of cattle during growth--are DLK1-positive cells the origin of marbling flecks?

  Elke Albrecht, Judith Kuzinski, Katrin Komolka, Takafumi Gotoh, Steffen Maak

  Meat science 100 237 - 45 2015/02 [Refereed][Not invited]
   

  Localization and abundance of early markers of adipogenic differentiation were investigated in bovine muscle tissue to verify their association with marbling development. Bovine skeletal muscle samples were used for immunohistochemical localization and measurement of mRNA and protein abundance of delta-like homolog 1 (DLK1) and CCAAT/enhancer-binding protein beta (CEBPB). The localization of DLK1-positive cells confirmed the position of small clusters of adipocytes which can be considered as the origin of marbling flecks. The results indicated higher DLK1 expression in the less marbled muscle of Holstein (HS) steers (P < 0.05) and a trend to higher CEBPB expression in Japanese Black (JB) steers (P < 0.1) at slaughter. The number of DLK1-positive cells and fat content were negatively correlated. The lower expression of DLK1 together with higher CEBPB abundance during fattening in JB may have contributed to the development of more adipocytes in the skeletal muscle of JB.
• Potential of the application of epigenetics in animal production

  Takafumi Gotoh

  Animal Production Science 55 (2) 145 - 158 1836-5787 2015 [Refereed][Not invited]
   

  Our many current environmental challenges, including worldwide abnormal weather, global warming, and pollution, necessitate a new and innovative strategy for animal production for the next generation. This strategy should incorporate not only higher-efficiency production, but also advanced biological concepts and multi-functional agricultural techniques, into environmentally friendly systems. Recent research has discovered a unique phenomenon referred to as 'foetal and neonatal programming', which is based on 'the developmental origins of health and disease (DOHaD)' concept. These studies have shown that alterations in foetal and early postnatal nutrition and endocrine status may result in developmental adaptations that permanently change the structure, physiology and metabolism of affected animals during adult life. Ruminants fill an important ecological niche that capitalises on the symbiotic relationship between fibre-fermenting ruminal microbes and the mammalian demand for usable nutrients. The timing of the perturbation in maternal nutrient availability plays an important role in determining the effect that the foetal and neonatal programming will have on the developing placenta or foetus and offspring performance. Developmental programming through nutritional manipulations may help the ruminant, as an effective grass-protein converter, fulfil its production potential.
• Studies on constitution regulation of Wagyu by metabolic programing

  後藤 貴文

  栄養生理研究会報 = Proceedings of Japanese Society for Animal Nutrition and Metabolism 家畜栄養生理研究会 59 (2) 69 - 78 0286-4754 2015 [Refereed][Not invited]
   

  現在、和牛仔牛価格が高騰しており、和牛生産の現場は混乱している。従来、40万円程度であった仔牛(和牛肥育素牛)1頭当たりの価格が、60万円を超えることが多くなった。和牛の肥育において、素牛の価格は、コストに大きく影響する。仔牛の高騰が始まる以前の平成25年度に発表された農林水産統計によると、平成25年度の生産価格は、仔牛1頭当たり59万2,996円であり、肥育牛(去勢若齢肥育牛)1頭当たり93万8,404円である。一方、販売による農家の粗収益は、仔牛が45万2,000円(平成25年3月)であり、肥育牛(去勢若齢肥育牛)は82万6,260円(平成25年1月のA-4レベルの平均枝肉卸売価格を平均枝肉重量470kgで算出)であった。仔牛の価格が数年前より20万円以上高騰している現状を考慮すると、和牛肥育農家の経営はかなり厳しいと推察される。農林水産省は、肉用牛肥育経営の安定を図るため、粗収益が生産コストを下回った場合に、生産者と国の積立金から差額の8割を補助金として交付する、いわゆるマルキン(肉用牛肥育経営安定特別対策)事業を実施しており、平成25年度は869億円の予算を組んだ。このように現状の和牛生産の現場は、混とんとして経営的に難しい状況が続いている。コスト高の要因としては、輸入穀物飼料の高騰と、農家の高齢化による繁殖農家戸数の減少が、結果として仔牛数の減少を導き、仔牛価格の高騰を招いている。
• Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese shorthorn cattle.

  H Takahashi, K Sato, T Yamaguchi, M Miyake, H Watanabe, Y Nagasawa, E Kitagawa, S Terada, M Urakawa, M T Rose, C D McMahon, K Watanabe, S Ohwada, T Gotoh, H Aso

  Domestic animal endocrinology 48 62 - 8 2014/07 [Refereed][Not invited]
   

  The purpose of this study was to determine whether myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese Shorthorn cattle. Plasma concentrations of glucose were lower in DM cattle than in NM cattle (P < 0.01). The expression of GLUT4 messenger RNA (mRNA) in the skeletal muscle ex vivo and in myoblasts at 72 h after differentiation in vitro was higher in DM cattle than in NM cattle (P < 0.01). In contrast, the NM and DM cattle did not differ with respect to skeletal muscle expression of GLUT1 and myocyte enhancer factor-2c (MEF2c), a transcription factor of GLUT4. In differentiated myoblasts, the expression of GLUT1, GLUT4, and MEF2c mRNAs was greater in DM cattle than in NM cattle (P < 0.01). In the presence and absence of insulin, glucose uptake in myoblasts was increased in DM cattle relative to that of NM cattle (P < 0.01). The addition of myostatin decreased the expression of GLUT4 and MEF2c mRNAs in DM myoblasts (P < 0.05). Results of the present study suggest that myostatin inhibits the expression of GLUT4 mRNA possibly via MEF2c and that the greater ability of the DM cattle to produce muscle relative to the NM cattle may be due to their greater sensitivity to insulin and greater use of glucose.
• Localization of Tektin 1 at both acrosome and flagella of mouse and bull spermatozoa.

  Sayoko Oiki, Erina Hiyama, Takafumi Gotoh, Hiroshi Iida

  Zoological science 31 (2) 101 - 7 2014/02 [Refereed][Not invited]
   

  Tektins (TEKTs) are constitutive filamentous proteins of microtubules in cilia, flagella, basal bodies, and centrioles. In mammals, five TEKTs (TEKT1, 2, 3, 4, and 5) have been identified in testis and spermatozoa. With the exception of TEKT1, these TEKTs have been reported to be present in spermatozoa with predominant localization at the peri-axoneme structures of flagella, i.e., mitochondria and outer dense fibers. In the present study, we produced an antibody against TEKT1 to examine the localization of TEKT1 in mouse, bull, and rat spermatozoa. By immunoblot analyses and immunofluorescence microscopy, we found TEKT1 to be present in sperm flagella and at the apical region of acrosome cap in spermatozoa of all these species. Acrosome-associated TEKT1 disappeared after in vitro acrosome reaction in mouse spermatozoa. These observations suggest another potential role for TEKT1 as a cytoskeletal element in the sperm head, or as a molecule involved in acrosome-related phenomena, such as acrosome reaction.
• Grazing Effects on Wild Boar Behavior at Abandoned Cultivated Land

  MARUI Atsushi, TOUDOU Nobuhiro, OKAYASU Takashi, GOTOH Takafumi, ETOH Tetsuji, SHIOTSUKA Yuji, TAKAHASHI Hideyuki

  Nihon Danchi Chikusan Gakkaihou 日本暖地畜産学会 57 (1) 17 - 22 2185-081X 2014 [Refereed][Not invited]
  
• Fatty acid profiles and adipogenic gene expression of various fat depots in Japanese Black and Holstein steers.

  Bungo Shirouchi, Elke Albrecht, Gerd Nuernberg, Steffen Maak, Samadmanivong Olavanh, Yoshinori Nakamura, Masao Sato, Takafumi Gotoh, Karin Nuernberg

  Meat science 96 (1) 157 - 64 2014/01 [Refereed][Not invited]
   

  Objective of the study was to assess the breed effect on fatty acid (FA) composition of different adipose tissues and on mRNA expression of genes involved in adipogenesis and fat metabolism. Japanese Black (JB) and Holstein (HS) steers were kept under equivalent conditions with high energy intake resulting in large differences in intramuscular fat (IMF) accumulation in longissimus muscle (LM). The relative FA composition of muscle, intermuscular fat, visceral fat, and perirenal fat was comparable between JB and HS steers. Circulating fatty acids were also similar in both breeds. Most relevant breed effects were identified in IMF, underlining the uniqueness of this adipose tissue site. JB steers had more monounsaturated FA and less saturated FA. Perilipin 1 and adipose differentiation-related protein (ADFP) mRNA levels were higher in IMF of JB. The results suggest advanced maturity of IMF cells in JB and altered local conditions in muscle influencing IMF accumulation and composition.
• Genetic Evaluation of Carcass Traits of Commercial Steers at Different Slaughter End-points

  Chang-Gwon Dang, Seung-Hwan Lee, Hyeong-Cheol Kim, Jeong-Mook Lee, Ki-Jun Jeon, Seong-Heum Yeon, Seong-Koo Hong, Hee-Sul Kang, Takafumi Gotoh, Jun-Heon Lee, Boh-Suk Yang

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 58 (2) 295 - 300 0023-6152 2013/09 [Refereed][Not invited]
   

  Carcass records, including pedigree, for 10,441 steers born from 2003 to 2008 and 21,711 animals total; were made available by the Pyengchang Youngwal Jungsun Livestock Cooperative. Data for each trait were adjusted for each of 5 end points: age (EPA), back fat (EPB), carcass weight (EPC), eye muscle area (EPE), and marbling score (EPM), and adjustments were made by fitting a linear. Genetic parameters were estimated using ASREML, which uses an average information algorithm. At different slaughter end points, heritability estimates for CWT (range=0.25 to 0.37) and EMA (range=0.17 to 0.33) were more significantly different than heritability estimates for BE (range=0.41 to 0.42) and MS (range=0.40 to 0.45). Genetic correlations between BF and CWT, EMA and CWT, and EMA and MS were positive, and those between BE and EMA and BF and MS were negative. The genetic correlation between CWT and MS yielded conflicting results. Correlations of sire breeding value for BF, CWT, EMA, and MS were in the range of 0.91 to 0.99, 0.75 to 0.93, 0.72 to 0.95, and 0.90 to 0.99, respectively, and those of the dam were in the range of 0.90 to 0.97, 0.73 to 0.94, 0.71 to 0.94, and 0.91 to 0.99, respectively. Further study will be needed to determine whether these alternative end points result in a more accurate estimate of BV than the traditional age end point.
• Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black male × Holstein female).

  F Ebara, S Inada, M Morikawa, S-H Asaoka, Y Isozaki, A Saito, T Etoh, Y Shiotsuka, S G Roh, J Wegner, T Gotoh

  Journal of animal physiology and animal nutrition 97 (4) 684 - 93 2013/08 [Refereed][Not invited]
   

  The objective was to investigate the impact of nutrient intake during the early growth period on the expression of glucose metabolism-related genes in skeletal muscle of cross-bred cattle. From 1.5 to 5 months of age, group H (n=7) animals were intensively fed a high-protein and low-fat milk replacer [crude protein (CP) 28%; ether extracts (EE) 18%; max: 2.0 kg, 12 l/day], and group R (n=7) animals were fed a restricted amount of normal milk replacer (CP 25%; EE 23%; max 0.5 kg, 4 l/day). From 6 to 10 months of age, group H cattle were fed a high-nutrition total mixed ration mainly prepared from grain feed, and group R cattle were fed only roughage. Blood samples were taken from each animal at three biopsy times (1.5, 5 and 10 months of age), and the blood plasma concentration of glucose and insulin was analysed. In glucose concentration, there were no significant differences; however, the concentrations of insulin were higher in group H than in group R at 5 and 10 months of age. Muscle samples were taken by biopsy from longissimus thoracis muscle (LT) at 1.5, 5 and 10 months of age. We analysed mRNA expression levels using the quantitative real-time polymerase chain reaction (PCR) assay for glucose transporters (GLUT1 and GLUT4), insulin receptor, phosphatidylinositol 3-kinase (PI-3K), protein kinase B (PKB, also known as Akt), hexokinase 1 (HK1) and tumour necrosis factor alpha (TNFα). Although no differences were detected at 1.5 and 5 months of age, at 10 months of age, GLUT1, HK1 and TNFα mRNA expression levels were significantly higher in group H than in group R. These results suggested Glut1 that affects insulin-independently mediated glucose uptake was more responsive to improved nutrition during early growth stage than GLUT4 that insulin-dependently mediated glucose uptake in LT of cattle.
• Effects of Calving Years, Times and Seasons on Milk Yield Traits in Turkish Brown Swiss Cows in a Steppe Climate

  Suleyman Cilek, Takafumi Gotoh

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 57 (2) 447 - 451 0023-6152 2012/09 [Refereed][Not invited]
   

  This study was conducted to investigate the environmental factors affecting milk yield traits of Turkish Brown cows raised at the Ulas state farm under a sub-tropical climate. First the effect of calving years on actual milk yield, daily milk yield, 305-day milk yield, 305-day mature age milk yield, lactation duration and dry period days were investigated. In 2000 to 2006, the actual milk yield, daily milk yield, 305-day milk yield, 305-day mature age milk yield, lactation duration and dry period days were significantly related to calving years. Second the effect of calving times on actual milk yield, daily milk yield, 305-day milk yield, 305-day mature age milk yield, lactation duration and dry period days were detected. These data indicate that the peak milk yield is at the 5th-7th calving times with maturity in Brown Swiss cows reared in the Central Anatolia region in Turkey. We carried out the effect of calving season on the identical items. The seasons of calving affect actual milk yield, days of lactation duration and clays of dry period. Cows that calve in winter have the highest actual milk yield. This could be due to stable nutrition during the important first lactation period to avoid cold stress. However, better management is required for cows calved in autumn that are under high temperature stress during an important lactation period.
• Stable carbon and nitrogen isotope analysis as a tool for inferring beef cattle feeding systems in Japan

  Yuta Yanagi, Hiroyuki Hirooka, Kazato Oishi, Yousuke Choumei, Hiroshi Hata, Mamoru Arai, Masayuki Kitagawa, Takafumi Gotoh, Sunao Inada, Hajime Kumagai

  Food Chemistry 134 (1) 502 - 506 0308-8146 2012/09 [Refereed][Not invited]
   

  A pilot study was conducted to evaluate the suitability of stable isotope analysis for inferring the feeding histories of cattle fed known feeds. Stable isotope ratios of carbon and nitrogen (δ 13C and δ 15N) were measured in meat and hair from cattle and in their feeds at five farms in different regions of Japan, and the correlations of the isotope ratios between meat and hair were analysed. The results showed that δ 13C values in feed depend on the photosynthesis type: C 3 or C 4. The values of δ 15N in feeds varied widely, indicating divergent feeds made from plant materials that have different nitrogen origins, such as soil, chemical fertilizer, manure and air. In both cattle meat and hair, the farms differed significantly in the values of δ 13C and δ 15N. Both δ 13C and δ 15N were significantly higher in hair than in meat, and high correlations between meat and hair in both δ 13C and δ 15N were found. The results suggested that stable carbon and nitrogen isotope analysis for cattle meat and hair could be used to trace the feeding histories of cattle in Japan, and that hair samples would be used as an alternative to meat. © 2012 Elsevier Ltd. All rights reserved.
• 反芻動物の培養肝細胞におけるchemerinの発現調節機構

  北山 峻, 菊池 佑弥, Ardiyanti Astrid, 山内 恵利, 後藤 貴文, 萩野 顕彦, 加藤 和雄, 盧 尚建

  東北畜産学会報 東北畜産学会 62 (2) 25 - 25 1341-626X 2012/08 [Refereed][Not invited]
  
• Technical note: Determination of cell-specific gene expression in bovine skeletal muscle tissue using laser microdissection and reverse-transcription quantitative polymerase chain reaction

  E. Albrecht, T. Gotoh, F. Ebara, J. Wegner, S. Maak

  JOURNAL OF ANIMAL SCIENCE 89 (12) 4339 - 4343 2011/12 [Refereed][Not invited]
   

  Skeletal muscle is a very heterogeneous tissue consisting of diverse cell types with specific transcription profiles. Therefore, the measured mRNA abundance of a certain cell type marker is influenced by the transcriptional activity as well as by the usually unknown number of contributing cells in the sample. In studies on the transcriptional activity of adipogenic genes, as indicators for the development of intramuscular adipocytes, an altered number of adipocytes or respective progenitor cells can mask changes in transcriptional activity. To overcome this problem, we started to use laser microdissection to isolate RNA of adipocytes and muscle fibers separately for downstream analysis. Even muscle fiber types can be collected and analyzed separately. Laser microdissection in combination with biopsy techniques enables gene expression studies of particular cell types during the life cycle of an animal. First experiences using laser microdissection for adipogenic gene expression studies in bovine skeletal muscle are described, and the influence of sample preparation and future challenges are discussed.
• Reproduction Characteristics in Brown Swiss Cows Reared Under Steppe Climate Conditions in Turkey

  Sueleyman Cilek, Takafumi Gotoh

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY 56 (2) 287 - 292 0023-6152 2011/09 [Refereed][Not invited]
   

  The objective of this study was to investigate the effects of environmental factors on reproductive traits of pure Brown Swiss cows reared under steppe climate conditions in the central Anatolia region of Turkey. In this study, 1502 reproductive records of Brown Swiss cows, which were reared in Ulas State Farm, were analyzed. The least squares means for gestation period, calving interval, non-pregnant period, insemination number for cows, insemination number per conception for heifers at first insemination, first insemination age, and age at first calving were 287 clays, 416 days, 128 days, 1.8 times, 1.6 times, 608 days, and 928 days, respectively. Calving times significantly affected the non-pregnant period, calving interval and gestation period (P<0.05). The calving intervals were significantly the longest in the 2(nd) calving time and the shortest in the 8(th) and 9(th) calving times (P<0.01). The birth season of heifers significantly affected first insemination age and age at first calving (P<0.01, P<0.001, respectively). For the calving season analysis, gestation period was the longest in autumn (P<0.01). The number of inseminations per conception for cows was significantly larger in spring and autumn than that in summer (P<0.01). The first calving ages were significantly earlier in autumn than in the other seasons (P<0.01). For analysis of insemination season, regarding insemination number per conception, first insemination age and first calving age, heifers in spring and summer were significantly larger than those in winter and autumn (P<0.01). These results suggested that 1) birth and insemination season affected first insemination age and first calving age, 2) Cows born in autumn and winter showed an earlier first insemination and first calving age than in spring and summer, and 3) the same trend would be observed for insemination season.
• Cellular conditions for intramuscular fat deposition in Japanese Black and Holstein steers.

  E Albrecht, T Gotoh, F Ebara, J X Xu, T Viergutz, G Nürnberg, S Maak, J Wegner

  Meat science 89 (1) 13 - 20 2011/09 [Refereed][Not invited]
   

  The experiment was conducted to study the development of intramuscular fat in Japanese Black (JB) compared to Holstein (HS) steers and to find breed differences for fat depot development and distribution in the carcass under equal feeding conditions. Additional to slaughter samples, biopsy samples of longissimus muscle (LM) and subcutaneous fat, taken at 10, 14, 18, and 22 months of age, were used for histological and molecular investigations. Japanese Black steers stored about 14% more fat in the LM (P = 0.001), resulting in larger marbling flecks (P < 0.001). Muscle fibers and intramuscular adipocytes in both breeds responded to the high energy feeding with significant enlargement, which was faster in JB. Histograms of intramuscular adipocytes size showed a shift toward larger cells during growth, but also the abundance of small, developing adipocytes. This development was accompanied by a correlated up-regulation of adipogenic genes until 22 months of age.
• Comparison of feeding systems: feed cost, palatability and environmental impact among hay-fattened beef, consistent grass-only-fed beef and conventional marbled beef in Wagyu (Japanese Black cattle).

  Khounsaknalath Sithyphone, Mitsuyasu Yabe, Hiroshi Horita, Keisuke Hayashi, Tomiko Fumita, Yuji Shiotsuka, Tetsuji Etoh, Fumio Ebara, Olavanh Samadmanivong, Jochen Wegner, Takafumi Gotoh

  Animal science journal = Nihon chikusan Gakkaiho 82 (2) 352 - 9 2011/04 [Refereed][Not invited]
   

  The objective of this article is to compare feed cost, palatability and environmental impacts among feeding systems of high concentrate (HC), high hay (HH) and grass-only-fed (Gof) groups. Feed cost was the sum of costs paid for feed intake times the price of feed per kilogram. Palatability was measured by a panel taste test using HH and Gof beef and analyzed for differences. Environmental impacts were calculated based on 1 kg of Japanese beef yield of CO(2) equivalents (eq) and animal end weights at each feeding stage. Results showed that the HH and Gof feeding systems could significantly reduce feed costs by approximately 60% and 78%, respectively, from the HC. In the panel taste test, 50% and 47.50% of panelists indicated that HH beef was 'extremely delicious' and 'acceptable,' respectively, while 15% indicated that Gof beef was 'extremely delicious'; 62.50% indicated that Gof beef was 'acceptable.' Environmental impacts of each feeding system in terms of CO(2) equivalents (eq) were 9.32, 6.10 and 2.04 tonnes of eq for the HC, HH and Gof, respectively. The HH was an economical system that produced moderate impacts on the environment and had impressive taste.
• Intensive nursing and feeding during the early growth period altered intramuscular adipogenesis in crossbred steers (Japanese Black male x Holstein female)

  Fumio Ebara, Sunao Inada, So-Hei Asaoka, Yoshihiro Isozaki, Akira Saito, Tetsuji Etoh, Yuji Shiotsuka, Takafumi Gotoh

  Journal of Animal and Veterinary Advances 9 (6) 982 - 989 1680-5593 2010 [Refereed][Not invited]
   

  We investigated the influence of feed quality during the early growth stage on expression of adipogenic genes in skeletal muscle of crossbred cattle. The cattle were divided three groups at 45 days of age group high energy (H: n = 7), group normal energy (N: n = 7) and group roughage (R: n = 7). Cattle in group H were three fold-intensively fed a high protein and low fat milk replacer (Crude Protein (CP): 28%, Ether Extracts (EE): 18%, max: 2.0 kg, 12 L day-1) from 45 days to 4 months of age. In groups N and R, cattle were fed a normal milk replacer (CP: 25%, EE: 23%, max: 0.5 kg, 4 L day-1) from 45 days to 4 months of age. From 4-10 months of age, cattle in group H were fed a high-nutrition Total Mixture Rate (TMR), while group N were fed a normal-nutrition TMR and group R were fed only roughage. Muscle samples were taken by biopsy from Longissimus thoracis muscle (LT) at 45 days, 4 and 10 months of age. Results from assays using quantitative real-time Polym erase Chain Reaction (PCR) revealed that gene expression of Peroxisome Proliferator-activated Receptor Gamma 2 (PPARγ2), CCAAT/Enhancer-Binding Protein alpha (C/EBPα), Stearoyl-CoA Desaturase (SCD), Glucose-6-Phosphate Dehydrogenase (G6PD), Fatty Acid Synthase (FASN) and Leptin related to adipogenesis and fatty acid synthesis differed among groups. The expression of these genes was significantly different among groups at 10 months but not at 4 months of age. These results demonstrated that differences in feed quality during early growth stages had substantial effects on the expression of genes related to adipogenesis and fatty acid synthesis in LT. © Medwell Journals, 2010.
• Intensified nursing dramatically accelerates growth performance and the size of the body frame in Japanese black and holstein crossbred steers

  Sunao Inada, Fumio Ebara, Sohei Asaoka, Kenichi Asada, Yoshihiro Isozaki, Akira Saito, Toshie Sugiyama, Takafumi Gotoh

  Journal of Animal and Veterinary Advances 9 (6) 1037 - 1047 1680-5593 2010 [Refereed][Not invited]
   

  Crossbred (Japanese Black male and Holstein female) steers were used to investigate the effects of feeding a large amount of high-protem milk replacer on growth performance, morphological change and myogenic regulation in skeletal muscle. Group HP (n = 7) was fed high-protein milk replacer (Crude Protein (CP): 29%, maximum intake 2.0 kg day-1) and group C (n = 14) was fed a standard milk replacer (CP: 26%, 0.5 kg day-1) from 56-147 days of age. Changes in Body Weight (BW), body frame measurements, plasma Insulin-like Growth Factor-1 (IGF-1) and plasma Alkaline Phosphatase (ALP) were investigated. M. Longissimus Thoracis (LT) samples were obtained by biopsy to investigate the myofiber type composition, diameter and the mRNA expression of Myogenic Regulatory related genes by quantitative real-time RT-PCR. A large amount of high protein milk replacer (Intensified Nursing) in group HP improved their synthesis of IGF-1 in the liver and accelerated skeletal development by maintaining high levels of plasma IGF-1 and ALP concentration, which was highly controlled by CP intake from diet. The myofiber type composition and size in the LT was not influenced by nutritional condition and aging during the nursing period. Intensified Nursing extends myofiber length rather than increasing the thickness of the myofiber, according to the increase in the size of the body frame during this phase. The expression of MyoD and IGF-1 receptors in the LT decreased at the early stage of the nursing period without the influence of nutritional condition. This suggested the change would control the level of differentiation from the myoblast to the myocyte. © Medwell Journals, 2010.
• Differences in muscle and fat accretion in Japanese Black and European cattle.

  T Gotoh, E Albrecht, F Teuscher, K Kawabata, K Sakashita, H Iwamoto, J Wegner

  Meat science 82 (3) 300 - 8 2009/07 [Refereed][Not invited]
   

  The development of different muscles and adipose tissues during growth was investigated in commercial Japanese Black (JB) cattle and compared with breeds of the largest variation to be found in Europe. Animals, reared under typical conditions for Japanese and European beef production systems, gained similar body weights but different carcass composition at 24months of age. The carcass of JB contained more adipose tissue and the least proportion of muscle. The longissimus muscle of JB developed extraordinary amounts of 23.3% intramuscular fat (IMF) at 24months of age, compared from 0.6% to 4.7% in European breeds. The relationships between IMF content in the longissimus muscle and different adipose tissue weights indicate that a large amount of "waste fat" is accreted with every percent of IMF. However in JB, the good ability of IMF deposition is associated with relatively least development of "waste fat", as a result of unique breed characteristics combined with special feeding system.
• Comparative observations of the growth changes of the histochemical properties and collagen architecture of the iliotibialis lateralis muscle from Silky, layer and meat type cockerels

  Ichiro Oshima, Hisao Iwamoto, Shoji Tabata, Yoshitaka Ono, Akira Ishibashi, Nobuya Shiba, Hideyuki Miyachi, Takafumi Gotoh, Shotaro Nishimura

  ANIMAL SCIENCE JOURNAL 78 (5) 546 - 559 2007/10 [Refereed][Not invited]
   

  Growth-related changes in the histochemical property and collagen architecture of the iliotibialis lateralis muscle were compared among Silky, layer and meat cockerels. Histochemical and immunohistochemical methods were employed to observe the collagen architecture. The total intramuscular collagen was also determined. The muscle consisted of type IIA, IIB and IIC myofibers, of which type IIB occurred at the highest frequency. The diameter of type IIB myofibers in each week was largest in the layer, followed by the meat, and was smallest in the Silky. The total amount of collagen reached 3.38 mg/g in the meat bird, 3.03 mg/g in the layer and 2.71 mg/g in the Silky by 30 weeks of age, respectively. In the perimysium, the collagen bundles increased in size and density of fibrils with growth. At 30 weeks of age the layer had compact collagen platelets while the Silky had loose collagen bundles. In the meat bird, the collagen bundles were moderately compact. The endomysial collagen network had a large mesh size at 1 week and thereafter accumulated many collagen fibrils to form a felt-like fabric of fibrils at 30 weeks of age. From these results it appears that growth-related changes in the iliotibialis lateralis muscle are not necessarily causally affected by the different growth rates of chicken breeds.
• Three-dimensional observation of connective tissue of bovine Masseter muscle under concentrate - and roughage-fed conditions by using immunohistochemical/confocal laser-scanning microscopic methods

  Y.-N. Nakamura, H. Iwamoto, T. Etoh, Y. Shiotsuka, T. Yamaguchi, Y. Ono, S. Tabata, S. Nishimura, T. Gotoh

  JOURNAL OF FOOD SCIENCE 72 (6) E375 - E381 0022-1147 2007/08 [Refereed][Not invited]
   

  We investigated changes in connective tissue components of masseter (MA) muscle in Japanese black heifers (n= 6) in concentrate- and roughage-fed groups (groups C and R, respectively). Body weight, at slaughter of experimental heifers in group C (272.3 +/- 22.3kg) was higher (P < 0.05) than that of group R(213.8 +/- 27.5 kg). However, muscle weight and myofiber diameter (superficial and deep layers) of MAmuscle did not differ between groups C and R. In contrast, total mastication duration of group R was longer (P < 0.05) than that of group C, MAmuscle of groups C and R was composed only of type I myofiber. Using immunohistochemical/confocal, laser-scanning microscopy , type I collagen was observed mainly in perimysium, and type V and VI collagen were observed in perimysium. and endomysium of both groups. Type IV collagen and laminin were observed only In the endomysium in both groups. However, type ill collagen and fibronectin were strongly apparent in the perimysium and endomysium in group R. Connective tissue components in the perimysium of groups C and R were observed to form plate-shaped layers. On the other hand, honeycomb-shaped connective tissue components were seen in the endomysium-surrounded muscle fibers. In particular, fibronectin was strongly observed in the perimysium and endomysium in group R. These results indicate that there are different developmental changes among connective tissue components in MA muscle in response to mastication. The immunohistochemical/confocal laser-scanning microscopic method Is useful to, investigate the structural relationship among connective tissue components In skeletal muscle.
• Three-dimensional reconstruction of intramuscular collagen networks of bovine muscle: A demonstration by an immunohistochemical/confocal laser-scanning microscopic method

  Yoshi-Nori Nakamura, Hisao Iwamoto, Takahiro Yamaguchi, Yoshitaka Ono, Yoshitaka Nakanishi, Shoji Tabata, Shotaro Nishimura, Takafumi Gotoh

  ANIMAL SCIENCE JOURNAL 78 (4) 445 - 447 1344-3941 2007/08 [Refereed][Not invited]
   

  We undertook a three-dimensional reconstruction of intramuscular collagen networks of bovine muscle using an immunohistochemical/confocal laser-scanning microscopic method. By immunohistochemical staining, type I and III collagens were observed mainly in the perimysium, while type IV collagen was observed in the endomysium. On the other hand, type V and VI collagens were observed in both the perimysium and endomysium. By confocal laser-scanning microscopy, the collagen observed in the perimysium was three-dimensionally reconstructed as plate-shaped layers whereas the collagen observed in the endomysium surrounded myofibers. The three-dimensionally reconstructed observations using immunohistochemical/confocal laser-scanning microscopic method is useful for investigating collagen networks in muscle.
• Effect of daytime grazing of fresh grass without supplemental feed on serum and milk a-tocopherol concentrations of holstein dairy cows

  Yoshi-Nori Nakamura, Akio Shiga, Shigeru Akasaka, Takafumi Gotoh

  Journal of Applied Animal Research 32 (2) 119 - 124 0974-1844 2007 [Refereed][Not invited]
   

  The effect of switching 5 Holstein dairy cows from concentrate-fed to roughage-fed conditions without supplemental feed for seven weeks on serum parameters and milk chemical composition in morning and afternoon milk samples was investigated. BW was not affected and blood hematocrit and total protein, glucose and triglyceride concentrations varied within normal values throughout the experiment. During day grazing period, serum non-esterified fatty acid (NEFA), milk somatic cell counts (SCC), total solids, lipids and milk and serum a-tocopherol values increased, whereas, those of milk yield, milk protein, lactose and solids-non-fat decreased. For diurnal changes, NEFA concentrations were lower, whereas, SCC, milk lipid and milk a-tocopherol concentrations were higher in the afternoon. Changes in milk chemical composition, when cows were switched from concentrate-fed to roughage-fed conditions including daytime grazing, were related to the chemical composition of the feeds consumed. © GSP, India.
• Sfrp 1 and Sfrp2 regulate anteroposterior axis elongation and somite segmentation during mouse embryogenesis

  Wataru Satoh, Takafumi Gotoh, Yasuhiko Tsunematsu, Shinichi Aizawa, Akihiko Shimon

  Development 133 (6) 989 - 999 0950-1991 2006/03 [Refereed][Not invited]
   

  Regulation of Wnt signaling is essential for embryonic patterning. Sfrps are secreted Wnt antagonists that directly interact with the Wnt ligand to inhibit signaling. Here, we show that Sfrp1 and Sfrp2 are required for anteroposterior (AP) axis elongation and somitogenesis in the thoracic region during mouse embryogenesis. Double homozygous mutations in Sfrp1 and Sfrp2 lead to severe shortening of the thoracic region. By contrast, a homozygous mutation in one or the other exerts no effect on embryogenesis, indicating that Sfrp1 and Sfrp2 are functionally redundant. The defect of a shortened thoracic region appears to be the consequence of AP axis reduction and incomplete somite segmentation. The reduction in the AP axis is partially due to abnormalities in cell migration of pre-somitic mesoderm from the end of gastrulation. Aberrant somite segmentation is associated with altered oscillations of Notch signaling, as evidenced by abnormal Lfng and Hes7 expression during somitogenesis in the thoracic region. This study suggests that Wnt regulation by Sfrp1 and Sfrp2 is required for embryonic patterning.
• Short-term response of vegetation to cattle grazing in an abandoned orchard in southwestern Japan

  K. Hayashi, K. Ikeda, A. Ueda, T. Fumita, T. Etoh, T. Gotoh

  Asian-Australasian Journal of Animal Sciences 19 (4) 514 - 520 1011-2367 2006 [Refereed][Not invited]
   

  An abandoned mandarin orange orchard in southwestern Japan was set-stocked by Japanese Black cows at two stocking rates (1.0 and 2.0 animals/ha), and vegetation dynamics and diet selection by cattle were monitored for two years, in an effort to obtain information on effective use of abandoned agricultural fields for low-cost animal production and environmental conservation. Two dominant species at the commencement of grazing, kudzu (Pueraria lobata Ohwi) and tall goldenrod (Solidago altissima L.), snowed different responses to grazing during the two years the composition of kudzu decreased, contrasting with that of tall goldenrod which increased at both stocking rates. This was caused by high preference for kudzu and avoidance or low preference for tall goldenrod by cattle. Retrogression of vegetation due to cattle disturbances occurred at both stocking rates, with the high stocking rate leading to a lower degree of succession than the low stocking rate. It was shown that cattle grazing, particularly at a high stocking rate, was effective in the management of vegetation of an abandoned orchard.
• Usefulness of Natural Mating on the Reproduction in Japanese Black Cows

  塩塚 雄二, 衛藤 哲次, 岩元 久雄, 後藤 貴文

  Bulletin of the Kyushu University Farm 九州大学農学部附属農場 (12) 60 - 65 1346-5643 2005/10 

  黒毛和種の種雄牛1頭と繁殖雌牛14頭を用いて，自然発情（自然発情区）後の自然交配と発情同期化処理に基づく発情誘起（発情誘起区）による自然交配における繁殖成績を調べ，人工授精における繁殖成績と比較検討した．発情同期化処理としてCIDR（イージーブリード）を利用したオブシンク（Ovsynch : Ovary Synchronization）プログラムを用いた．それぞれの延受胎率は自然交配の自然発情区93.3％，発情誘起区50.0％，人工授精の自然発情区42.4％，発情誘起区40.0％を示し，受胎した繁殖牛1頭当たりの授精回数はそれぞれ1.1回，2.0回，2.4回，および2.5回であった．自然交配の自然発情区では14頭中13頭が初回授精にて受胎した（92.9％）．また，自然交配の受胎間隔は人工授精のそれより有意に短縮され（83日短縮），345日であった（P＜0.01）．黒毛和種において種雄牛による自然交配により繁殖成績の向上，繁殖管理の省力化が図られ，同一種雄牛で同一月齢の子牛生産が可能になると推察された．黒毛和種種雄牛の飼養管理にかかる年間経費は，年間40頭の子牛を受胎させる繁殖経費とほぼ等しく，種雄牛による自然交配の導入は大規模繁殖農家にとって有効であると考えられる．今後は黒毛和種による自然交配で生産された子牛に対する市場評価を高めることが課題であろう．Reproductive efficiency of a Japanese Black bull to 14 cows was examined on the mating in natural estrus and introduced estrus caused by Ovsynch (Ovary Synchronization) program with CIDR (EAZI-BREED) for synchronization of estrus. The efficiency of the mating was compared with it of artificial insemination (AI) service. Conception rate on the mating to natural estrous cows was 93.3%, the number of fertilization per one conception was 1.1 times, and the scores on the mating to Ovsynch-treated cows were 50.0% and 2.0 services respectively. On AI service, conception rate in natural estrus was 42.4%, the number of fertilization per one conception was 2.4, and the scores in introduced estrus were 40.0% and 2.5 services respectively. 13 of 14 cows were conceived by the first mating in natural estrus. Moreover, conception interval on natural mating (345 days) was shorter than on AI services (428 days). From these result, it is suggested that natural mating by bull is quite useful to improvement of reproductive efficiency and labor-saving management of reproduction and make it possible to breed calves whose father is the same at the same season. On the reproductive cost, the introduction of bull is effective for farmers who raise more than 40 calves a year. To realize this natural mating in Japanese Black cows, the estimate of its produced calf would be essential on the market.
• Viability of Echinochloa crusgalli (L.) Beauv. and Digitaria adscendens (H.B.K.) Henr. Seeds Excreted in Feces from Cattle

  Ikeda Kentarou, Hayashi Keisuke, Gotoh Takafumi, Etou Tetsuji, Tobisa Manabu, Shimojo Masataka, Masuda Yasuhisa

  Japanese Journal of Grassland Science 日本草地学会 51 (2) 171 - 178 0447-5933 2005 [Refereed][Not invited]
   

  When hay is contaminated by many seeds of Echinochloa crus-galli (L.) Beauv. and Digitaria adscendens (H.B.K.) Henr., it is possible that these seeds disseminate over cultivation fields through the feces of cattle eating this hay. The purpose of this study is to estimate the number of seeds of both weeds in hay (Exp. 1), and to clarify which digestive organ affects the germination of the awakened and dormant seeds in vitro and in vivo (Exp. 2). The number of seeds of both weeds in hay increased as the coverage of both weeds increased. It was estimated that 10,000 seeds of both weeds were included in 1 kg of the hay from a meadow dominated by both weeds. Artificial rumen fluid alone did not affect the germination of both weeds, while acid-pepsin fluid depressed the germination largely. Awakened seeds of both weeds collected from cattle's feces showed slight germination. However, dormant seeds of D. adscendens germinated more than 20%, and those of E. crus-galli germinated to the same percentage as before being fed to the cattle. From the above-mentioned results, it appears that a large number of germinable seeds were excreted into cattle's feces, especially in the case of dormant seeds.
• 牛の体質制御によるグラスフェッド型牛肉生産システム再構築

  後藤 貴文, 文田 登美子, 林 恵介

  栄養生理研究会報 家畜栄養生理研究会 49 (2) 33 - 48 0286-4754 2005 [Refereed][Not invited]
  
• ウシの消化管通過に伴う牧草種子の発芽能力の変化

  池田 堅太郎, 板山 智子, 中村 好徳, 植田 彩, 林 恵介, 衛藤 哲次, 後藤 貴文, 岡野 香, 岩元 久雄, 増田 泰久

  日本草地学会誌 50 120 - 121 2004/03/26 [Refereed][Not invited]
  
• Effect of Different Light Quality on the Germination of Echinochloa crus-galli (L.) Beauv. seeds and Digitaria adscendens (H.B.K) Henr. Seeds

  Ikeda Kentarou, Hayashi Keisuke, Eto Tetsuji, Gotoh Takafumi, Tobisa Manabu, Shimojo Masataka, Masuda Yasuhisa

  Japanese Journal of Grassland Science 日本草地学会 49 (6) 640 - 644 0447-5933 2004 [Refereed][Not invited]
   

  In order to examine the effect of different light quality on the germination of Echinochloa crus-galli (L.) Beauv. seeds and Digitaria adscendens (H.B.K.) Henr. seeds, the germination test was carried out under the conditions of green light, red light, far-red light, filtrated light through cheese cloth, full natural light, or dark. In addition, to examine whether phytochrome function is involved in the light effect on termination of both species, another germination test was carried out using seeds under the alternate radiation of red and far-red lights. In both species, the germination percentage was in the order of light≒filtrated light through cheese cloth≒redlight>green light>far-red light≒dark, there were significant differences among light qualities (p<0.05). In addition the promoting effect of red light on germination of both species was negated by far-red light. Oppositely the effect of far-red light on germination of both species was negated by red light. These results suggest that the promoting effect of lighton the germination of both species depends on red light. Also there is a strong possibility that phytochrome function is involved in the light germination of both species.
• Changes of histochemical profiles of myofibers in pectoralis and supracoracoideus fasciculi induced by breeding for large or small body size in Japanese quails

  Hisao Iwamoto, Takafumi Gotoh, Shoji Tabata, Shotaro Nishimura, Satoru Okamoto

  Animal Science Journal 74 (2) 111 - 118 1344-3941 2003/04 [Refereed][Not invited]
   

  The histochemical profiles of myofibers in Musculus pectoralis (PT) and M. supracoracoideus (SC) fasciculi were compared among Japanese quail strains with large, normal and small body sizes. In male and female adults, both the PT and SC muscles had attained a 2.5-2.7-fold weight gain in the large strain and conversely a 0.43-0.50-fold change in the small strain relative to those of the normal size. The muscles were composed of fasciculi with a central cluster of type IIA fibers surrounded by a peripheral layer of type IIB fibers. In the large strain, the cross sectional area (CSA) of the fasciculus and CSA of the fibers in each type were significantly enlarged compared with those in the normal size, with the exception of the fasciculus in the deep region of the male PT muscle. The hypertrophied type IIA fibers in the large strain showed considerable variation in nicotinamide adenine dinucleotide dehydrogenase activity, some of which might represent a transitional form into type IIB fibers. In the small strain, the fasciculus CSA did not significantly differ from that of the normal size except for the PT surface region of the male. However, fiber atrophy was observed in type IIB fibers of the PT surface region in both sexes, and type IIA fibers of the PT deep region and SC muscle in the small strain male quails. The relative fiber type composition of a fasciculus in each region showed only a slight change across the strains. These results indicate that breast muscle hypertrophy in the large strain could be based mainly on fasciculus and fiber hypertrophy, but muscle atrophy in the small strain is not induced by fasciculus and fiber atrophy.
• The pattern of grazing behaviour and blood condition of the Japanese Black Cattle in the abandoned groves

  林 恵介, 宮川 創, 衛藤 哲次, 後藤 貴文, 増田 泰久

  Bulletin of the Kyushu University Farm 九州大学農学部附属農場 (11) 36 - 45 1346-5643 2003/03 

  果樹園跡地における黒毛和牛放牧システムを確立することを目的とし，放牧牛の血液性状および体重から健康状態を調査し，さらに放牧牛の行動と植生の関係について検討を行った．かつてミカンの栽培が行われていた果樹園跡地約1.86haに黒毛和種繁殖雌牛3頭（平均87ヵ月齢，平均体重544kg）を2001年8月から放牧した．その結果，放牧開始から3日間において急激な体重の減少（27.7±7.6kg）とカテコールアミン（アドレナリン，ノルアドレナリン）の血中濃度の上昇が見られた．しかし，その後放牧牛の体重および採食時間，反芻時間は速やかに増加した．放牧牛はクズを主に採食し，その結果放牧開始53日目にはクズはほとんど見られなくなった．また，53日目の採食時間は他の調査日とは異なり，GT/RT（採食時間／反芻時間）値も他の調査日とは異なっていた．したがって，GT／RT値の変化は採食構成の変化を示す可能性が推察された．本実験において放牧牛が果樹園跡地のような新しい環境に速やかに馴致することが明らかとなった．果樹園跡地を牛肉生産の場として長期利用するためには適正な放牧圧や植生動態に及ぼす放牧牛の嗜好性の影響についての検討が必要である。The objective of this study was the establishment of the grazing system of Japanese Black Cattle in the area of abandoned groves. We investigated the body condition of cattle from blood samples, and the relationship between grazing behaviour and vegetation in the abandoned grove. In August 2001, three heads of Japanese Black Cattle aged 87 months with 544 kg in average weight were transported and introduced into the experimental site that was about 1.86 ha. There were an immediate decrease in body weight (27.7±7.6kg) and increase in the level of catecholamines (adrenaline and noradrenaline) for the first 3 days. After that, however, the body weight, the grazing and the rumination time of the cattie rapidly increased. The cattle grazed mainly kudzu, therefore on the 53rd day, the mass of kudzu markedly declined. Interestingly the grazing time on the 53rd day was different from that on the other researching days. The value of GT (grazing time)/ RT (rumination time) also showed differences between 53rd day and the other days. The change of GT/RT value may be indicative of the transition of the herbage components. It was clear that the cattle rapidly adapted to the new environment like such abandoned grove. The long time use of abandoned groves for beef cattle production requires the investigation into the proper grazing pressure and herbage preference by cattle under vegetation dynamics.
• Influence of Light and Temperature on Germination of Echinochloa crus-galli (L.) Beauv. and Digitaria adscendens (H.B.K.) Henr. seeds

  IKEDA Kentarou, GOTOH Takafumi, TOBISA Manabu, SHIMOJO Masataka, MASUDA Yasuhisa

  Japanese Journal of Grassland Science 日本草地学会 49 (1) 28 - 32 0447-5933 2003 [Refereed][Not invited]
   

  The interactive effect of light and temperature on germination of Echinochloa crus-galli (L.) Beauv. and Digitaria adscendens (H.B.K.) Henr. was studied using their seeds given cold stratification for 4 months. Both species showed higher germination percentages under the higher light intensity than under the lower light intensity. The light was more effective for germination under the lower temperature (15℃, 20℃ and 25℃) than under the optimum temperature (30℃). Effects of lighting length on germination of both species were determined under 25℃, and it was found that only 10 minutes light flash everyday was sufficient for the germination. Alternating temperatures in a day, such as 25℃ (8h)/10℃ (16h), and 25℃ (8h)/5℃ (16h), were effective in promoting the germination in darkness. However, the small range of alternating temperature, such as 25℃ (8h)/20℃ (16h), was hardly effective in promoting the germination. Both species could germinate under favorable alternating temperature conditions without the light flash (25℃ (8h)/10℃ (16h) in the dark, and 25℃ (8h)/5℃ (16h) in the dark) as well as under the low or constant temperature with the adequate light (15℃ under 100% relative light intensity, and 25℃ with more than 10 minutes/day light flash). Probably light flash and alternating temperature are not always necessary for the germination. Both factors may only assist the germination when the condition is inadequate for germination such as low temperatures or low light intensities.
• Studies on Dormancy Awakening and Germination of Echinochloa crus-galli (L.) Beauv. and Digitaria adscendens (H. B. K.) Henr. Buried Seeds in the Central Highland Area of Kyushu

  IKEDA Kentarou, GOTO Takafumi, TOBISA Manabu, SIMOJO Masataka, MASUDA Yasuhisa

  Japanese Journal of Grassland Science 日本草地学会 49 (3) 238 - 242 0447-5933 2003 [Refereed][Not invited]
   

  Studies were carried out on the progress of dormancy awakening and changes of effect of light and alternating temperature on germination of Echinochloa crus-galli (L.) Beauv. and Digitaria adscendens (H. B.K.) Henr. buried seeds in the central highland area of Kyushu. Time of dormancy awakening was not different between shedding and adhesion seeds of both species. Buried seeds of both species remained in dormant during the season in which the environment was suitable for germinate in the soil, i. e., September and October. The dormancy was gradually awakened during winter. Over 85 percent of the seeds had come out of dormancy by May. Under preservative conditions of different temperature and moisture, progress of dormancy awakening was different. Chilling in moist condition promoted the dormancy awakening most, which maybe the most important environmental factor related to the dormancy awakening in winter. Effect of light and alternating temperature on germination was very different among various dormancy awakening phases. In the phase of deep dormancy no germination occurred under any conditions, but in the more progressed phase of awakening germination was promoted under light and daily alternating temperature conditions. Finally germination could be observed even under constant temperature and dark conditions in the completely awaked phase. Difference of dormancy awakening phase caused by various environmental conditions in the soil may be one of the factors that induce variation in germination time.
• Germination Time of Digitariaadscendens (H.B.K.) Henr. and Echinochloa crus-galli (L.) Beauv. as Influenced by Bare Ground or by Covering with Dactylis glomerata L. and its Cutting Treatments

  IKEDA Kentaro, GOTO Takafumi, TOBISA Manabu, SHIMOJO Masataka, MASUDA Yasuhisa

  Japanese Journal of Grassland Science 日本草地学会 49 (4) 373 - 378 0447-5933 2003 [Refereed][Not invited]
   

  Studies were carried out in the central highland area of Kyushu to observe the germination of Da (Digitaria adscendens (H.B.K.) Henr.) and EC (Echinochloa crus-galli (L.) Beauv.) as influenced by vegetation and cutting management. We made three-treatments plots using containers ; the plot without plant (bare ground plot), the plot planted with stubbles ofDactylis glomerata L. with cutting two times on July 4. and Aug. 15. (cutting plot) and without cutting (no cutting plot). The number of seedlings of both species and relative light intensity on the soil surface were investigated every two weeks from May 9. to Sep. 12. At the end of the experiment, the number of germinable buried seeds were counted. The soil surface temperature was measured during 3 days before and after each cutting. The total number of seedlings during the experimental period was in the order of the bare ground plot>the cutting plot>the no cutting plot. In the bare ground plot, the largest number of seedling was observed in the middle of June for Da and from the end of May to the middle of June for EC. In the cutting plot, many seedlings were observed in both species during the first two weeks following cutting. Intensive germinations were not observed in both species in no cutting plot. The number of germinable buried seeds of both species at the end of the experiment in the no cutting plot was significantly (p<0.05) larger than that of the cutting plot and the bare ground plot where no germinable buried seeds were found. It was considered that the increase in relative light intensity and the widened diurnal range of surface soil temperature after cutting induced the germinations in both species.
• ヤギを放牧した荒廃果樹園の植生変化

  城戸 英, 石若 礼子, 飛佐 学, 重盛 進, 後藤 貴文, 増田 泰久

  日本草地学会九州支部会報 日本暖地畜産学会 33 (2) 23 - 29 2003 [Refereed][Not invited]
  
• 果樹園跡地の未利用草資源を利用した牛肉生産システムの再構築

  後藤 貴文

  日本草地学会九州支部会報 日本暖地畜産学会 33 (1) 18 - 25 2003 [Refereed][Not invited]
  
• 2-15 変温と光質の違いがイヌビエとメヒシバの発芽に及ぼす影響

  池田 堅太郎, 松藤 由貴子, 荒牧 春香, 塩塚 雄二, 衛藤 哲次, 西村 光博, 後藤 貴文, 増田 泰久

  日本草地学会誌 日本草地学会 48 (0) 92 - 93 0447-5933 2002 [Refereed][Not invited]
  
• 2-16 九州中部高原地域の採草地におけるイヌビエとメヒシバ埋土種子の休眠覚醒の進行と発芽特性の変化

  池田 堅太郎, 松藤 由貴子, 荒牧 春香, 塩塚 雄二, 衛藤 哲次, 西村 光博, 後藤 貴文, 増田 泰久

  日本草地学会誌 日本草地学会 48 (0) 94 - 95 0447-5933 2002 [Refereed][Not invited]
  
• 荒廃果樹園におけるヤギの採食による植生の変化について

  城戸 英, 重盛 進, 飛佐 学, 増田 泰久, 宮川 創, 林 恵介, 佐藤 洋行, 松藤 由貴子, 浅野 陽樹, 池田 堅太郎, 石若 礼子, 後藤 貴文

  日本家畜管理学会誌 日本家畜管理学会 38 (1) 40 - 41 1342-1131 2002 [Refereed][Not invited]
  
• Scanning electron microscopic observation of the architecture of collagen fibres in chicken M-iliotibialis lateralis

  H Iwamoto, S Tabata, K Sakakibara, S Nishimura, T Gotoh, Y Koga

  BRITISH POULTRY SCIENCE 42 (3) 321 - 326 0007-1668 2001/07 [Refereed][Not invited]
   

  1. The collagen architecture of M. iliotibialis lateralis in chicken was observed under the scanning electron microscope after muscle maceration in NaOH. 2. Immunohistochemical methods showed Type I and III collagens to be distributed over both perimysium and endomysium. 3. Thick perimysium around secondary myofibre fasciculi was composed of many large longitudinal collagen bundles and a few small circumferential bundles. In contrast, thin perimysium around primary myofibre fasciculi showed mainly circumferential bundles. 4. Endomysium had a honeycomb-like structure and consisted of a fine collagen mesh, its main fibre striation being circumferential. 5. It is suggested that functional demand differs between thick perimysium and thin endomysium.
• Proportional change of somatotrophs, mammotrophs and somatomammotrophs induced by cessation of lactation in female goat adenohypophysis.

  Nishimura,S, Ikeda,K, Okano,K, Gotoh,T, Tabata,S, Iwamoto,H

  Animal Science Journal Japanese Society of Animal Science 72 (1) 32 - 33 1346-907X 2001 [Refereed][Not invited]
   

  In the goat adenohypophysis, percentages of somatotrophs, mammotrophs and somatomammotrophs were compared between the lactating females and the post-lactation immunohistochemically. The pituitary weight was significantly different between the lactating females (0.65±0.10g) and the past-lactation (0.38±0.07g). Except for zona tuberalis, mammotrophs appeared at significantly larger percentage in the lactating females (26.9%) than in the post-lactation (10.1%) and conversely somatomammotrophs in the post-lactation (28.1%) than in the other (16.0%) at 5% level. The percentage of somatotrophs increased only in the caudal region of the pars distalis but immunonegative cells did not show significant differences after the cessation of lactation. From these results, it was suggested that a part of mammotrophs might transform into somatomammotrophs after cessation of lactation in the female goat adenohypophysis.
• Testis developments and puberty in the male Tokara (Japanese native) goat

  S Nishimura, K Okano, K Yasukouchi, T Gotoh, S Tabata, H Iwamoto

  ANIMAL REPRODUCTION SCIENCE 64 (1-2) 127 - 131 0378-4320 2000/12 [Refereed][Not invited]
   

  In male Tokara (Japanese native) goats, testis development and the onset of puberty Were studied histologically and observing sexual behaviour. Testes weight increased from 36 +/- 5.3(S.E.) g (n = 5) at 3 months of age to 126 +/- 6.3 g (n = 6) at 12 months. The degree of the testis development differed among kids at 3 months of age and only one of four had produced spermatozoa in the test is. Large number of spermatozoa were always present in seminiferous tubules and epididymal ducts from 4 months of age. The diameter of seminiferous tubules increased from 133 +/- 9.9 mum (n = 4) at 3 months to 198 +/- 1.0 mum (n = 3) at 6 months with little increase thereafter. Mounting and pelvic thrusts onto a teaser doe started at from 9 to 14 weeks of age. Ejaculated semen contained sperm with good motility for the first time from 17 weeks. The male Tokara goat reaches puberty at around 4 months of age but testis development continues to 12 months. (C) 2000 Elsevier Science B.V. All rights reserved.
• Myofibre composition and total collagen content in M-iliotibialis lateralis and M-pectoralis of Silkie and White Leghorn chickens

  K Sakakibara, S Tabata, N Shiba, T Gotoh, S Nishimura, H Iwamoto

  BRITISH POULTRY SCIENCE 41 (5) 570 - 574 0007-1668 2000/12 [Refereed][Not invited]
   

  1. Using adult Silkie and White Leghorn (WL) chickens, fibre composition and total collagen content were compared between M. iliotibialis lateralis (ITL) and M. pectoralis (PT). 2. Fibres were divided into type IIR and IIW showing strong and weak reduced nicotinamide adenine dinucleotide dehydrogenase activities, respectively. 3. Fibre composition differed markedly between ITL and PT muscle. ITL muscle was composed of both types IIR and IIW fibre but PT muscle of only type IIW. Breed difference on composition was noted only in ITL muscle of cocks (type IIR; Silkie 53.8% and WL 37.0%). 4. Total collagen content was greater in ITL muscles and in cocks in both muscles. However, a breed difference was observed only in the PT muscle of hens. 5. In conclusion, the total collagen content was affected by muscle location within the body and by sex rather than by fibre composition.
• Histochemical Studies on the Skeletal Muscles in Fattened Mishima Steers

  MORITA Satoru, IWAMOTO Hisao, FUKUMITSU Yuji, GOTOH Takafumi, NISHIMURA Shoutarou, ONO Yoshitaka

  日本畜産學會報 = The Japanese journal of zootechnical science 71 (7) J51 - J59 1346-907X 2000/02/25 [Refereed][Not invited]
  
• 去勢雄見島牛の骨格筋に関する組織化学的研究

  守田 智, 岩元 久雄, 福満 裕二, 後藤 貴文, 西村 正太郎, 尾野 喜孝

  Animal Science Journal John Wiley & Sons Australia, Ltd 71 (7) J51 - J59 1344-3941 2000/02 [Refereed][Not invited]
  
• Heterogeneous composition of histochemical fibre types in the different parts of M-longissimus thoracis from Mishima (Japanese native) steers

  S Morita, H Iwamoto, Y Fukumitsu, T Gotoh, S Nishimura, Y Ono

  MEAT SCIENCE 54 (1) 59 - 63 0309-1740 2000/01 [Refereed][Not invited]
   

  In Mishima (Japanese native) steers, histochemical properties of m. longissimus thoracis were examined at 3 parts: on the level of 6th thoracic (LT I), 11th thoracic (LT II) and 5th lumbar vertebra (LT III). Myofibres were categorized into Type I, II A and II B. The same fibre type composition (I, 37%, II A, 17%, II B, 46%) was observed at LT I and LT III and another (I, 26%, II A, 15%, II B, 59%) at LT II. At each part apparent regional differences of fibre type composition could not be demonstrated except for Type I between medial and lateral subpart at LT I and Type II A between central and lateral at LT III because of its marked variation among the individual steers. Relative fibre diameter of Type I to Type II A in the combined data was large at LT I and LT III. Type II B fibres showed larger diameter than Type I only at LT III. From these results it was suggested that Mishima steers have maintained another histochemical property of the longissimus muscle differing from that of Japanese Black steers (Gotoh, Iwamoto, One, Nishimura, Matsuo, Nakanishi, Umetsu & Takahara, (1994). Comparative study on the regional composition of fiber types in M. Longissimus thoracis with different marbling scores for Japanese Black steers. Animal Science and Technology, 65, 454-453). (C) 1999 Elsevier Science Ltd. All rights reserved.
• Bovine circumvallate taste bud: the structure and the gustducin localization

  TABATA Shoji, KOBAYASHI Takahiko, NISHIMURA Shotaro, GOTOH Takafumi, MUGURUMA Michio, IWAMOTO Hisao

  The Japanese journal of taste and smell research 日本味と匂学会 6 (3) 537 - 540 1340-4806 1999/12/01 [Refereed][Not invited]
  
• 黒毛和種若齢去勢牛の体各部位骨格筋における組織化学的特性

  後藤 貴文, 岩元 久雄, 中西 良孝, 梅津 頼三郎, 尾野 喜孝

  Animal Science Journal 70 (6) 497 - 509 1344-3941 1999/11 [Refereed][Not invited]
  
• 黒毛和種の若齢去勢牛における大腿二頭筋前部の筋線維型構成について

  後藤 貴文, 岩元 久雄, 中西 良孝, 梅津 頼三郎, 尾野 喜孝

  Animal Science Journal 70 (6) 510 - 518 1344-3941 1999/11 [Refereed][Not invited]
  
• 去勢雄見島牛の大腿二頭筋前部における筋線維型の分布の差異

  守田 智, 岩元 久雄, 福満 裕二, 後藤 貴文, 西村 正太郎, 尾野 喜孝

  Animal Science Journal John Wiley & Sons Australia, Ltd 70 (9) J177 - J183 1344-3941 1999/08 [Refereed][Not invited]
  
• Quantitative and Qualitative Beef Production Ability of Single-calf Japanese Black Heifers.

  Ono Yoshitaka, Eto Tetsuji, Uchida Tetsuro, Nishimura Teruhiro, Gotoh Takafumi, Iwamoto Hisao, Morita Satoru

  The West Japan Journal of Animal Science 日本暖地畜産学会 (42) 23 - 27 0914-3459 1999 [Refereed][Not invited]
   

  This study was performed to evaluate the beef production ability of single-calf heifers (SH), by comparing to those of adult none-calf heifers (AH) and young heifers (YH) in Japanese Black cattle. SH and AH were fattened from 32 and 30 age of month for 5 months, respectively, and YH was fattened from 10 to 25 age of month. Although SH and All had a larger pre-slaughter body weight than YH (P<0.05), there were no differences in carcass weight and dressing percentage among the groups. No significant differences were also observed in the other carcass yield traits (rib eye area, rib thickness, backfat thickness and cutability) and the quality traits (marbling, beef color, fat color and firmness) among the groups. From these results, it is suggested that SH would be able to produce the almost same size of carcasses and meat quality as YH in Japanese Black cattle.
• Growth Characteristics of Fattening Single-calf Japanese Black Heifers.

  Ono Yoshitaka, Eto Tetsuji, Uchida Tetsuro, Nishimura Teruhiro, Gotoh Takafumi, Iwamoto Hisao, Morita Satoru

  The West Japan Journal of Animal Science 日本暖地畜産学会 (42) 5 - 11 0914-3459 1999 [Refereed][Not invited]
   

  This study was conducted to evaluate the growth characteristics of fattening single-calf heifers of Japanese Black.Four single-calf heifers (SH) and 3 adult none-calf heifers (AH) were fattened for 5 months. Six young heifers (YH) were engaged in the fattening course from 10-to 25-month old, and incorporated into this study for the last 5 months. Body weight, height and length, chest and trunk girths, shoulder, chest and thurl widths were measured monthly, and relative growth rates of body dimensions were compared among the 3 heifer groups using the growth coefficients of the 7 measured items to the body weight. In spite of smaller body weights, SH and AH obtained a larger body weight than YH by the end of the fattening period.Average daily gain during the fattening period was 1.33, 1.06 and 0.49kg, in SH, AH and YH, respectively. The body weight gain in SH and AH was associated with the growth of chest and trunk girths, shoulder, chest and thurl widths. On the other hand, the growth of chest and trunk girths and chest width was related to the body weight gain in YH. The growth of body height and length was very little and never contributed to body weight gain in all 3 groups. From these results, it is suggested that SH shows a remarkable growth based on the growth of body width, and could reach a marketable final body weight after only 5 months of fattening.
• Comparative Studies on the Fiber Type Composition in the Different Parts of M. biceps femoris of Mishima Steers

  MORITA Satoru, IWAMOTO Hisao, FUKUMITSU Yuji, GOTOH Takafumi, NISHIMURA Shoutarou, ONO Yoshitaka

  Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 70 (9) 177 - 183 1346-907X 1999 [Refereed][Not invited]
   

  In Mishima steers, regional differences of histochemical fiber type composition were demonstrated in the biceps femoris muscles. The cranial portion of the biceps femoris muscle was divided into 3 parts with equal width and moreover into s subparts with equal depth in each part. Type/βR, αR and αW fibers were categorized. The subparts of each part did not show any differences of fiber type composition except for the distal part which showed a little difference. Prominent change of the composition was recognized from the proximal part to the middle where Type, BR fibers decreased from 47.8%to 27.0% and conversely Type αW increased from 23.7% to 42.7%. However, relative diameter of Type βR fibers to the other types was large in the Mishima.Our results indicate that enough volume of the biceps femoris muscle of the Mishima has remained showing its ability to produce good meat.
• Myfiber type distribution in the Cranial portion of M. biceps femoris from Japanese Black Steers

  GOTOH Takafumi, IWAMOTO Hisao, NAKANISHI Yoshitaka, UMETSU Raizaburo, ONO Yoshitaka

  Animal Science Journal 70 (6) 510 - 518 1346-907X 1999 [Refereed][Not invited]
  
• Inter- and intra-breed variation in the fiber type composition of M.longissimus lumborum and M.biceps femoris in Japanese Black, Japanese Brown, Holstein and F1(Japanese Black X Holstein) steers

  IWAMOTO Hisao, GOTOH Takafumi, NISHIMURA Shotaro, ONO Yoshitaka, TAKAHARA Hitoshi

  Animal Science Journal 70 (6) 490 - 496 1346-907X 1999 [Refereed][Not invited]
  
• Histochemical properties of Skeletal muscles in different Body parts of young Japanese Black Steers

  Gotoh,T, Iwamoto,H, Nakanishi,Y, Umetsu,R, Ono,Y

  Animal Science Journal 70 (6) 497 - 509 1346-907X 1999 [Refereed][Not invited]
  
• 生検筋肉材料の組織学および組織化学的特質による枝肉成績予測の可能性

  守田 智, 岩元 久雄, 後藤 貴文, 西村 正太郎, 尾野 喜孝

  肉用牛研究会報 66 33 - 34 0386-8419 1998/12/25 [Refereed][Not invited]
  
• Effects of parent Shamo cocks on the histochemical properties of M-iliotibialis lateralis and M-supracoracoideus on their crossbred broilers

  H Iwamoto, F Katoh, T Gotoh, S Nishimura, Y Ono, Y Nishio, E Fukuhara, T Murakami

  BRITISH POULTRY SCIENCE 39 (5) 589 - 595 0007-1668 1998/12 [Refereed][Not invited]
   

  1. Four Shame (a Japanese game bird) cocks showing different characteristics in the histochemical properties of M. iliotibialis lateralis (ITL) were crossed with White Rock hens to produce male and female crossbred broilers of the 4 lines (90 d of age). Normal broilers (56 d) were used, for comparison. 2. Histochemical properties of ITL and M. supracoracoideus (SC) were compared among the crossbred lines and normal broilers. Myofibres were divided into Types II R, II I and II W showing high, moderate and low reduced nicotinamide adenine dinucleotide dehydrogenase (NADH-DH) activities, respectively. 3. In the ITL of the crossbred cockerels, the percentage of Type II R and II I fibres decreased and conversely Type II W increased in comparison to those in the Shame. 4. Sex differences of the histochemical properties were recognised only in the ITL of the crossbred, in which the percentage of Type II R fibres was greater in the male. 5. The different characteristics of the parent Shame cocks were reproduced only in the different fibre type composition of the ITL muscle in the crossbred cockerels. 6. The histochemical features of fibre type seemed to develop with bird age, particularly subsarcolemmal accumulation of formazan granules (indicating high NADH-DH activity) in Type IIR fibres. 7. Breed, line, sex and age differences in the histochemical properties were demonstrated clearly in ITL but not in SC.
• Comparative Studies of Muscle Histochemical Characteristics of Ducks between Free Ranged and Housed, and among Three Types of Breeds

  ONO Yoshitaka, GOTO Takafumi, NASU Ryou, IWAMOTO Hisao, TAKAYAMA Koji, NAKANISHI Yoshitaka, MANDA Masaharu

  Japanese poultry science 日本万国家禽学会 35 (6) 367 - 375 0029-0254 1998/11/25 [Refereed][Not invited]
   

  This study was conducted to compare the development and histochemistry of pectoralis and iliotibialis lateralis muscles between different raising systems of ducks; free ranged in paddy fields and housed, and among three types of breeds; Crossbred (Mallard×Khaki Cambell), Indian Runner and Chinese native. Three birds of both sexes from each breed at the age of 12 days were introduced into the paddy field (4a) at the first week after rice planted, kept for 72 days, and then transported into the pen where the birds were fed with formula diet for layers. The rest of the birds were fed with the same formula diet from the beginning of the experiment in the pen (1.9a). All the birds were killed at 21 weeks of age. The results obtained were follows;
  1. Body weight and the weights of pectoralis and iliotibialis muscles did not show any differences between free ranged and housed ducks, but breed differences were observed. Body weight was the greatest in Chinese native, and followed by Indian Runner and Crossbred (Mallard×Ckhaki Cambell). The pectoralis muscle was most developed in Crossbred (Mallard×Khaki Cambell), and the iliotibialis lateralis muscle was in Indian Runner.
  2. Both the muscles were composed of only type II fibers with a high contraction speed. Type II fibers were subdivided into two types;1) type II A fibers with high oxidative enzyme activity, 2) type II B fibers with low oxidative activity. Thepectoralis muscle was composed of around 70% type II A fibers and 30% type II B fibers. The iliotibialis lateralis muscle was composed of around 40% type II A fibers and 60% type II B fibers. The housed Chinese native had more type II A fibers and less type II B fibers in the iliotibialis lateralis muscle than the free ranged. Among the breeds, Crossbred (Mallard×Khaki Cambell) possessed the highest percentage of type II A fibers, and the lowest of type II B fibers in the pectoralis muscle. Indian Runner had the highest percentage of type II A fibers, and the lowest of type II B fibers in the iliotibialis lateralis muscle.
  3. The muscle fiber diameter was smaller in type II A fiber than type II B fibers in both the muscles. In comparison among the breeds, Crossbred (Mallard×Khaki Cambell) and Indian Runner had a larger diameter of type II B fibers in the pectoralis muscle. In the iliotibialis lateralis muscle, Indian Runner and Chinese native had a larger diameter of both type II A and II B fibers.
  4. These results suggest that the effects of the different raising systems on muscle development of ducks were a little, and a higher flying ability in Crossbred (Mallard×XKhaki Cambell), running or walking ability on the ground in Indian Runner and meat productivity in Chinese native ducks.
• Interrelationships between the Histological Properties of Biopsy Muscle Materials and Beef Marbling Score in the Japanese Black Steers

  IWAMOTO Hisao, GOTOH Takafumi, NISHIMURA Shoutarou, TAKAHARA Hitoshi

  Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 66 (9) 807 - 809 1346-907X 1995/09/25 [Refereed][Not invited]
   

  Fifteen Japanese Black steers engaged in progeny test of two sires were used in this study. Histological properties of biopsy materials taken from longissimus lumborum and biceps femoris muscles were observed at 9-10 months old. Intramuscular fatty tissue, perimysium deposits, was detected already at this age with individual difference in its development. In the longissimus, the histological volume score of the fatty tissue was related significantly to beef marbling score (BMS) of the carcass at 21-22 months old (p<0.05). Correlation coefficient between percentage of Type αW fibers and BMS was also significant (p<0.05). In the biceps, these coefficients were not significant. These results indicated that BMS could be estimated from the early observation of the perimysium deposits in the longissimus muscle.
• Comparative Study on the Regional Composition of Fiber Types in the Cranial Portion of M. Biceps Femoris in Japanese Black Steers

  GOTO Takafumi, IWAMOTO Hisao, ONO Yoshitaka, NISHIMURA Shotaro, SHIGEMATSU Ichiro, NAKANISHI Yoshitaka, UMETSU Raizaburo, TAKAHARA Hitoshi

  Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 66 (1) 62 - 71 1346-907X 1995/01/25 [Refereed][Not invited]
   

  A variation in fiber type distribution between regions was clarified in the cranial portion of the M. biceps femoris of Japanese Black steers. The cranial portion was divided into three main parts (proximal, middle and dista^l) and 15 subparts (5 arranged with respect to the superficia-lprofound subpart in each part). Muscle fibers were classified into type βR, αR and αW fibers according to the nomenclature of ASHMORE et al.1).
  Among the proximal, middle and distal parts, the percentage of Type βR fibers was higher in the proximal than in the middle and distal parts, while Type αR fibers showed a converse tendency. The percentage of Type αW fibers was highest in the distal part and lowest in the proximal part.
  Among proximal subparts, the fiber type percentages did not show any difference. Among the middle subparts, however, the percentage of Type βR and αW fibers was higher in the superficial than in the profound subparts, while that of Type αR fibers exhibited a converse tendency. Among the distal subparts, the percentage of Type αW fibers was higher in the superficial than in the profound subpart and that of Type αR fibers was lower in the superficial than in the profound subparts.
  These results indicate different localizations of fiber types among parts and subparts of the cranial portion of the M. biceps femoris, and functional differentiation relative to the region. It is suggested that the proximal part mainly maintains posture and that the distal part is active in hind leg locomotion.
• 黒毛和種去勢雄牛の脂肪交雑度の異なる胸最長筋における筋線維型構成の比較

  後藤 貴文, 岩元 久雄, 尾野 喜孝

  日本畜産学会報 (公社)日本畜産学会 65 (5) 454 - 463 1346-907X 1994/05 [Refereed][Not invited]
  
• 夏山冬里飼養方式における妊娠末期牛への夕方給餌が昼間分娩に及ぼす影響

  中西 良孝, 衛藤 哲次, 河村 俊久, 後藤 貴文, 増田 泰久, 梅津 頼三郎

  西日本畜産学会報 日本暖地畜産学会 (37) 54 - 56 0914-3459 1994 [Refereed][Not invited]
   

  夏山冬里方式で飼育された黒毛和種繁殖牛について, 妊娠末期における1カ月の夕方給餌 (16: 30) が昼間分娩に及ぼす影響を慣行給餌 (朝夕2回) の場合と比較検討した。放牧期での昼間 (5: 00～22: 00) 分娩に対する夕方給餌の効果は認められなかった。一方, 舎飼い期では, 夕方給餌を行った場合の昼間 (5: 00～22: 00) 分娩率は100%であり, 慣行給餌の場合の50%と比較して有意に高い値を示した (P<0.02) 。また, 夕方給餌を行った牛の昼間分娩率について, 放牧期と舎飼い期との比較を行うと, 舎飼い期の方が有意に高かった (P<0.05) 。しかし, 昼間分娩時間帯を8: 00～17: 00とした場合には, 舎飼い期に夕方給餌を行っても分娩率は50%となり, 慣行飼育牛と比べ若干高かったものの, 有意差は認められなかった。
• Different growth rates of male chicken skeletal muscles related to their histochemical properties

  H. Iwamoto, T. Gotoh, Y. Ono, H. Takahara

  British Poultry Science 34 (5) 925 - 938 1466-1799 1993 [Refereed][Not invited]
   

  1. Early, M. pubo-ischio-femoralis pars medialis (PIF muscle) and late, M. iliotibialis lateralis pars postacetabularis (ITL muscle), maturing muscles were studied. These two muscles contained different populations of histochemical fibre types. 2. The profile measurements of the muscles showed diphasic allometric growth relative to the weights. In the early stage of growth (up to 15 weeks after hatching), the muscle length, width and depth all increased, while in the later stage (from 15 to 35 weeks) muscle growth resulted mainly from the marked increase in the depth and to a lesser degree, from an increase in the width. 3. Type I fibres, observed predominantly in PIF muscle matured earlier than the Type II fibres of ITL muscle. 4. From these results, the detailed process of skeletal muscle growth in the chicken was discussed. © 1993, Taylor … Francis Group, LLC. All rights reserved.
• Distribution of Histochemical Fiber Types in M. biceps femoris of Crossbred Steer (Japanese Black Male×Holstein Female)

  GOTO Takafumi, IWAMOTO Hisao, ONO Yoshitaka, NISHIMURA Shotaro, TAKAHARA Hitoshi

  Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 64 (9) 927 - 929 1346-907X 1993 [Refereed][Not invited]
  
• The Distribution of Muscle Weight in the Tokara Goat and its Sex Differences

  NISHIMURA Shotaro, ONO Yoshitaka, GOTO Takafumi, IWAMOTO Hisao, TAKAHARA Hitoshi

  Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 64 (8) 826 - 832 1346-907X 1993 [Refereed][Not invited]
   

  In this study the distribution of skeletal muscle in the Tokara goat, a Japanese native breed, was examined and its sex difference was also discussed. The body and carcass weights of adult male (n=6) and female (n=6) goats (16-24months oad) were weighed. According to anatomical location, the skeletal muscle was divided into 3 groups, namely trunk, forelimb and hindlimb, and moreover totally into 10 subgroups made up from 3-4 in every group. The muscle weights in the 3 groups and 10 subgroups were weighed on the right side of the carcass, and total muscle weight was calculated. Thirty two individual muscles were removed and weighed on the left side of the carcass. As body weight exhibited very large differences between sexes (male; 31.0kg, female; 21.9kg), the carcass and total muscle weight did also. The development of the trunk and forelimb muscle group with respect to body weight differed prominently between sexes but not so for hindlimbs. When the compositional distribution of muscle was compared between sexes by its percentage weight of total muscle weight, the male gave larger values in the cervical group of the trunk and shoulder gurdle's group of forelimb, and the female gave larger values in the pelvic, femoral and crural groups of hindlimb, the scapular group of the forelimb and the abdominal group of the trunk. From these results, the characteristics of muscle distribution and meat production capability of the Tokara goat were clarified and also discussed in comparison with results reported for other ruminants.
• The Effect of Denervation on the Degenerative Process of Type I Fibers in the Biceps Brachii Muscle of the Chick

  ONO Yoshitaka, GOTO Takafumi, NISHIMURA Shotaro, IWAMOTO Hisao, TAKAHARA Hitoshi

  Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 63 (8) 805 - 808 1346-907X 1992 [Refereed][Not invited]
  
• The Effect of Different Management Practices on Incidence of Diarrhea of Naturally Suckled Japanese Black Calves

  NAKANISHI Yoshitaka, UMETSU Raizaburo, GOTO Takafumi

  The West Japan Journal of Animal Science 日本暖地畜産学会 (35) 16 - 21 0914-3459 1992 [Refereed][Not invited]
   

  Incidences of calf diarrhea between 0 and 91 days of age were investigated under two different management systems; (i) conventional system: old calving pen, disinfection of pen barn using invert soap solution (once/mo.) and keeping a group of 4-8 cow-calf pairs in loose barn, (ii) modified system: new calving pen away from loose barn with the old one, disinfection of pen barn using invert soap or orthodichlorobenzene solution (twice/mo.), dipping in an operator's foot bath and keeping a group of 4-6 cow-calf pairs in loose barn. Calves experimented under both systems were natural all suckled Japanese Black Cattle, which were born in the period from April to November. The number of calves used were 15 (8 heifers and 7 bulls) under conventional system (1989) and 10 (4 heifers and 6 bulls) under modified one (1990), respectively. The calves were kept in individual calving pens for the first week postpartum and then transferred to the loose barn (adjacent to the old calving pen) where they remained as a cow-calf group to the end of experiment. The relationship between the days of diarrhea morbidity and average daily weight gain in the calves during 0-91 days postpartum were also examined in both systems. The results obtained were as follows:
  The overall incidence rate for calf diarrhea showed a lower value in modified system than in conventional one (80 vs 100%) . Especially, the incidence rate for calf diarrhea for the first week postpartum in the new calving pen was much lower than that in the old pen (20 vs 53.3%) . In both conventional and modified systems, the incidence rates for calf diarrhea revealed the highest value (100 and 70%, respectively) during 0-30 days postpartum, thereafter tended to decline with advancing calf age until 91 days. The calves which had not suffered from diarrhea in the new calving pen, had a much shorter diarrheal duration even in the loose barn (average 2.4 days/animal) compared to the animals which had (and not) suffered from the illness in the old pen (average 18.6 and 7.4 days/animal, P<0.01 and P<0.05, respectively) . In the conventional system, a significant negative correlation was shown between diarrheal duration and average daily weight gain in calves (P<0.10), indicating that the calves which suffered from diarrhea for the longest time tended to have a slower growth rate. By contrast, it was suggested that in the modified system, a shorter diarrheal duration (within 7 days during the over all nursing period) in calves did not affect their performance.
• 新参牛を夜間に導入した場合の導入前後における牛群の行動比較 : 維持行動について

  中西 良孝, 河村 俊久, 後藤 貴文, 梅津 頼三郎, 衛藤 哲次

  日本家畜管理研究会誌 日本家畜管理研究会（現 日本家畜管理学会） 28 (1) 16 - 17 0916-6505 1992 [Refereed][Not invited]
  
• Comparative Studies on the Composition of Muscle Fiber Types in Japanese Black, Japanese Brown and Holstein Steers

  IWAMOTO Hisao, ONO Yoshitaka, GOTO Takafumi, NISHIMURA Shotaro, NAKANISHI Yoshitaka, UMEZU Raizaburo, TAKAHARA Hitoshi

  Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 62 (7) 674 - 682 1346-907X 1991 [Refereed][Not invited]
   

  Comparative studies on histochemical fiber type composition were carried out on eighteen skeletal muscles in six Japanese black, five Japanese brown and five Holstein steers. Muscle materials were excised and made into serial frozen cross sections which were stained with histochemical reactions for ATPase and NADH-dehydrogenase activity. Muscle fibers were distinguished into type βR, αR and αW fibers according to the nomenclature of ASHMORE et al.²⁾.M.longissimus thoracis, M. biceps femoris, M. semimembranosus, M. triceps brachii/Caput longum, M. semitendinosus and M. gluteus medius, which play an important role in producing meats in quantity because of their larger weights, were composed of all three types of fiber and exhibited obvious differences between the Black and other two breeds in the percentage distribution of each type of fiber. The larger percentage of αW fibers was observed in the Brown and Holstein, and conversely the larger percentage of βR or αR fibers was observed in the Black than in any other breed. The Black had larger βR fibers in higher frequency than the other two breeds did in M. longissimus thoracis, M. semimembranosu, M. triceps brachii/Caput longum, M. gluteus medius, and M. vastus medialis. The results of this experiment show that these muscles of the Blaek have a different fiber type composition from those of the other two breeds. Good potential for quantitative meat production of the highest grade of meat, such as loin and fllet which are mainly composed of M. longissimus thoracis and M. iliopsoas respectively, was indicated by the Brown, but not by the Holstein.
• Comparison of Histochemical Fiber Composition in M. longissimus thoracis and M. psoas major between Japanese Black and Japanese Brown Steers

  ONO Yoshitaka, IWAMOTO Hisao, GOTO Takahumi, NISHIMURA Shotaro, TAKAHARA Hitoshi, UMEZU Raizaburo, NAKANISHI Raizaburo

  Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 60 (9) 891 - 893 1346-907X 1989 [Refereed][Not invited]
  

Books etc

• メディカル・サイエンス・ダイジェスト2023年７月号 特集：人生最初の１０００日間と疾患感受性 妊婦の栄養摂取からみた次世代の健康

  合田 敏尚 (DOHaD理論の農学への応用と発展へ向けて)
  ニューサイエンス社 2023/06
• DOHaD先制医療への展開

  日本DOHaD学会, 佐田, 文宏, 福岡, 秀興 (7章 DOHaDを基盤とする動物・細胞モデル. 9. DOHaDの畜産への応用)
  金原出版 2023/05 (ISBN: 9784307170796) viii, 307p
• 食の現代社会論 : 科学と人間の狭間から

  伏木, 亨 (第３部 第11章 植物資源循環をめざす次世代型牛肉生産―牛肉におけるFun to Eat)
  [農山漁村文化協会] 2022/08 (ISBN: 9784540204098) 1オンラインリソース (442p)
• 電波技術協会報FORN ３４５号

  (スマートフォンで牛を飼う～DXで牛肉生産システムの構造改革～)
  一般財団法人 電波技術協会 2022/03
• 食の現代社会論

  伏見亨 (Joint work第11章 植物資源循環をめざす次世代型牛肉生産―牛肉におけるFun to Eat.)
  一般社団法人 農山漁村文化協会 2022 (ISBN: 9784540201097)

Conference Activities & Talks

• 加工型畜産から環境と調和したタンパク質生産へ向けた畜産のパラダイムシフト  [Invited]

  後藤貴文

  2023年度日本農学会シンポジウム「激動する社会と農学」東京大学弥生講堂  2023/10
• DOHaDに基づいた家畜生産研究２:和牛放牧肥育における度フォワード・エピジェネティクス研究  [Not invited]

  西野大地, 後藤貴文

  第1回動物生産におけるDOHaDと免疫および遺伝的はいけいの関係性シンポジウム 帯広畜産大学  2023/09
• これからの畜産研究、特に大家畜研究をどのように進化させればよいのか？  [Not invited]

  後藤貴文

  第1回動物生産におけるDOHaDと免疫および遺伝的はいけいの関係性シンポジウム 帯広畜産大学  2023/09
• 母牛の低栄養は胎子の肝臓における糖代謝、尿素回路、ステロイド代謝に影響を及ぼす  [Not invited]

  室谷進・Y Zhang, 乙丸孝之助, 大島一修, 大島一郎, 佐野光枝, 盧尚建, 尾嶋孝一, 後藤貴文

  第131回日本畜産学会 帯広畜産大学  2023/09
• 妊娠期の栄養環境がウシ胎仔の胸最長筋におけるＤＮＡメチル化に及ぼす影響  [Not invited]

  西野大地, 室谷 進, 後藤貴文

  第131回日本畜産学会 帯広畜産大学  2023/09
• 先端技術と国土をフル活用した放牧型牛肉生産システムの開発への挑戦  [Invited]

  後藤貴文

  第476回月例研究会 一般社団法人 日本科学飼料協会 オンライン  2023/05
• Potential application of epigenetics in Wagyu Production for the improvement of meat quality and consumer acceptance.  [Invited]

  Takafumi Gotoh

  The proceeding of the 55th Korean Society for Food Science of Animal Resources(KoSFA) International Symposium and Annual Meeting Gwangju, Korea.  2023/05
• 持続可能な社会に向けた食と農業のありかた、持続可能なタンパク源の生産  [Invited]

  後藤貴文

  一般社団法人 山岡記念財団 第6回科学技術講演会  2023/04
• Overcoming future industry challenges  [Invited]

  Takafumi Gotoh

  WagyuEdge2023 AWA conference in Sydney, Australia  2023/04
• 西南暖地畜産の未来に向けて 持続可能な未来型畜産の構築に向けた取り組み 教育研究・産業・行政からの提言  [Invited]

  後藤貴文

  持続可能な未来型畜産の構築に向けた畜産教育研究. 第15回日本暖地畜産学会鹿児島大会シンポジウム  2022/10
• 宇宙で加速する、スマート農業とサステナブル社会  [Invited]

  後藤貴文

  北海道宇宙サミット 2022 帯広市  2022/09
• 先端科学における牛肉生産の大革新への挑戦～和牛で放牧肥育の限界を超えられるか～  [Not invited]

  後藤貴文

  畜産経営経済研究会9月例会 online  2022/09
• 激動の時代の中でZ世代に対する大学における家畜生産実習をどのように位置づけるか  [Invited]

  後藤貴文

  令和5年畜産学教育協議会シンポジウム「畜産学教育における家畜生産実習について」帯広畜産大学  2022/09
• おいしい牛肉をつくるための和牛代謝プログラミングは可能か？：和牛における妊娠期の栄養が胎児の脂質代謝や骨格筋形成に与える影響  [Invited]

  後藤貴文

  第46回日本女性栄養・代謝学会  2022/09
• MATERNAL NUTRITION DURING GESTATION ALTERS FETUS MUSCLE TISSUE HISTOCHEMICAL PROPERTIES, AND MRNA AND MICRORNA EXPRESSION IN FETUS MUSCLE TISSUE OF WAGYU (JAPANESE BLACK) CATTLE  [Not invited]

  Yi Zhang, Kounosuke Otomaru, Kazunaga Oshima, Yuji Goto, Ichiro Oshima, Susumu Muroya, Mitsue Sano, Sanggun Roh, Takafumi Gotoh

  he proceedings of DOHaD World Congress 2022 in Vancouver, Canada
• 1,4 Effects of early high nutrition related to metabolic imprinting events on comprehensive DNA methylation of longissimus muscle in grass-fed Wagyu  [Not invited]

  Daichi Nishino, Sithyphone Khounsaknalath, Kunihiko Saito, Akira Saito, Tsuyoshi Abe, Shinobu Yasuo, Takafumi Gotoh

  68th international congress of meat science and technology Online, Kobe, Japan
• Potential of grass-fed Wagyu and application of epigenetics in beef production.  [Invited]

  Takafumi Gotoh

  The proceedings of 68th international congress of meat science and technology (ICoMST )
• 放牧におけるDXのポテンシャルと食肉生産  [Invited]

  後藤貴文

  日本学術会議公開シンポジウム「グリーン・デジタルによる持続的食料生産：環境保全型ＤＸ支援放牧のポテンシャル」  2022/03
• 放牧で美味しく健康的な黒毛和牛を育てる技術  [Invited]

  後藤貴文

  第11回「食と健康に関するシンポジウム」  2021/11
• 家畜生産へのDOHaD仮説の逆説的応用の可能性  [Invited]

  後藤貴文

  第45回日本女性栄養・代謝学会学術集会／第10回日本DOHaD学会学術集会  2021/09
• Changing production system in Japan  [Invited]

  Takafumi Gotoh

  WAGYUEDGE CONFERENCE  2021/04
• ウシにおける代謝プログラミングの可能性

  後藤貴文

  The 128th Annual Meeting of Japanese Society of Animal Science  2021/03  九州大学（オンライン）  Japanese Society of Animal Science
  
• 「畜産王国・九州のブランド確立を支える試験研究の取組 」

  後藤貴文

  第128回日本畜産学会 市民公開シンポジウム２（総合司会者（座長））  2021/03  九州大学（オンライン）  Japanese Society of Animal Science
  
• 「地球規模で考える放牧による牛肉生産の意義と未来」

  後藤貴文

  第27回日本産肉研究会、学術集会  2021/03  オンライン  The Japan Society for Sustainable Meat Production
   

  鹿児島大学 産肉研副会長
• 植物資源循環を目指す次世代型牛肉生産

  後藤貴文

  2020年度「食の文化フォーラム」40周年記念II 「食と現代」  2021/03  オンライン  味の素食の文化センター
  
• Effect of poor maternal nutrition on skeletal muscle microRNA expression of foetal calves 539

  S. Muroya, Y. Zhang, A. Kinoshita, K.Oshima, Y. Gotoh, I. Oshima, K. Otomaru, M. Sano, S. Roh, M. Futohashi, M.Oe, K. Ojima and T. Gotoh.

  European Association for Animal Production (EAAP) Annual Meeting 2020 (online)  2020/12
• Effects of serums derived from Wagyu calves fed different nutrition on adipocyte differentiation.

  R. Saneshima, S. Shimamoto, D. Ijiri, A. Ohtsuka, Y. Okamura, T, Gotoh

  European Association for Animal Production (EAAP) Annual Meeting 2020(online)  2020/12
• Potential of metabolic programming in beef production.  [Invited]

  Gotoh, T

  2020 international conference of the cooperation and integration of industry, education, research and application. Neo-agriculture education & intelligent animal husbandry  2020/09
• Effects of maternal nutrition on skeletal muscle and adipose tissue in fetuses of Wagyu cattle and its potential for sustainable meat production.  [Invited]

  後藤貴文

  日本生理学会 100周年記念大会

Works

• Molecularbiological and morphological aspects of meat quality in Japanese cattle

  2000 -2006
• Molecularbiological and morphological aspects of meat quality in Japanese cattle

  2000
• 口之島野生化牛骨格筋の組織化学的特徴

  1997
• Histochemical properties of muscle in Kuchinoshima cattle

  1997
• 日本ウズラ骨格筋の組織化学的特徴

  1996
• 黒毛和牛骨格筋の組織化学的特徴

  1996
• Histochemical properties of muscle in Japanese Quail

  1996
• Histochemical properties of muscles in Japanese Black cattle

  1996

MISC

• 妊娠期の栄養環境がウシ胎仔の胸最長筋におけるDNAメチル化に及ぼす影響

  西野大地, 室谷進, 後藤貴文  日本畜産学会大会講演要旨  131st-  2023
• 母牛の低栄養は胎子の肝臓における糖代謝,尿素回路,ステロイド代謝に影響を及ぼす

  室谷進, ZHANG Y, 乙丸孝之介, 大島一修, 大島一郎, 佐野光枝, 廬尚健, 尾嶋孝一, 後藤貴文  日本畜産学会大会講演要旨  131st-  2023
• 母牛の低栄養状態における胎仔胸最長筋のメタボローム解析

  室谷進, ZHANG Yi, 木下葵衣, 乙丸孝之介, 大島一修, 後藤裕司, 大島一郎, 佐野光枝, 盧尚健, 大江美香, 尾嶋孝一, 後藤貴文  日本畜産学会大会講演要旨  129th-  2021
• 新しい子牛の科学 : 胎生期から初産分娩まで

  猪熊壽, 加藤敏英, 後藤貴文, 佐藤繁, 芳賀聡, 松﨑正敏, 監修  緑書房  2021
• 新しい酪農経営のかたち : ジャージー牛のミルクと肉を丸ごと楽しむ経営 安原氏の挑戦

  後藤 貴文  畜産の情報 = Livestock industries information  (361)  48  -54  2019/11  [Not refereed][Not invited]
  
• 国内情報 放牧型畜産への先端技術の利活用 : IT技術を活用した高度放牧管理システム

  後藤 貴文  畜産技術 = Livestock technology  (772)  27  -33  2019/09  [Not refereed][Not invited]
  
• 牛肉の風味に影響を与える脂肪以外の因子の解明―筋線維タイプに着目した解析―

  間島大介, 岩井芳夫, 後藤貴文, 澤野祥子, 辰巳隆一, 中村真子, 水野谷航  日本畜産学会大会講演要旨  125th-  206  2019/03/28  [Not refereed][Not invited]
  
• スマート畜産:IoT・人工知能およびロボット技術の利活用 放牧型畜産への利活用 IT技術を活用した高度放牧管理システム

  後藤 貴文  日本畜産学会大会講演要旨集  125回-  84  -84  2019/03  [Not refereed][Not invited]
  
• 和牛の胎仔発生に与える母体栄養の影響(Effects of maternal nutrition on fetal development in Wagyu cows)

  Yi Zhang, 實島 伶奈, 長尾 有希子, 木下 葵衣, 大島 一修, 後藤 裕司, 大島 一郎, 佐野 光枝, 室谷 進, 盧 尚建, 太箸 誠, 岡村 保子, 乙丸 孝之介, 後藤 貴文  日本畜産学会大会講演要旨集  125回-  169  -169  2019/03  [Not refereed][Not invited]
  
• 新しい畜産経営のかたち : 株式会社重次郎 中屋敷氏の挑戦

  後藤 貴文  畜産の情報 = Livestock industries information  (348)  39  -47  2018/10  [Not refereed][Not invited]
  
• 黒毛和種仔牛の腸内環境と下痢症発症に関する遺伝学的分類とその評価

  中吉 晃祐, 松浦 真紀子, 宮本 浩邦, 小野瀬 暁, 岡田 徹, 衛藤 哲次, 塩塚 雄二, 藤野 亮一, 児玉 浩明, 後藤 貴文, 高橋 秀之  日本畜産学会大会講演要旨集  124回-  125  -125  2018/03  [Not refereed][Not invited]
  
• 哺乳期の日長が黒毛和種の成長や骨格筋関連指標に及ぼす影響

  安尾 しのぶ, 打和 龍宏, 高木 望, 古閑 弘晃, 志賀 灯, 高橋 秀之, 後藤 貴文, 古瀬 充宏  日本畜産学会大会講演要旨集  124回-  189  -189  2018/03  [Not refereed][Not invited]
  
• 黒毛和牛のグラスフェッド型牛肉のポテンシャルを向上させる代謝プログラミング

  後藤 貴文  食肉の科学  59-  (2)  137  -142  2018  [Not refereed][Not invited]
  
• 井信行氏の牛肉の生産哲学と阿蘇の畜産への展望 : 褐毛和種やジャージー種牛肉生産への道

  後藤 貴文  畜産の情報 = Livestock industries information  (336)  47  -54  2017/10  [Not refereed][Not invited]
  
• 胎児期と初期成長期の代謝プログラミングによる和牛肥育への応用 : 無駄な飼料と時間を減らすための肉質制御肥育期間の大幅な短縮技術開発は可能か?(下)

  後藤 貴文  畜産コンサルタント  53-  (9)  81  -85  2017/09  [Not refereed][Not invited]
  
• 児期と初期成長期の代謝プログラミングによる和牛肥育への応用 : 無駄な飼料と時間を減らすための肉質制御肥育期間の大幅な短縮技術開発は可能か?(上)

  後藤 貴文  畜産コンサルタント  53-  (8)  83  -85  2017/08  [Not refereed][Not invited]
  
• 好熱菌Bacillus hisashii BP-863の経口給与が仔牛の腸内環境に与える影響

  中吉 晃祐, 高橋 秀之, 宮本 浩邦, 加藤 完, 中西 裕美子, 大野 博司, 小野瀬 暁, 岡田 徹, 古閑 弘晃, 衛藤 哲次, 塩塚 雄二, 藤野 亮一, 児玉 浩明, 古瀬 充宏, 後藤 貴文  日本畜産学会大会講演要旨集  122回-  122  -122  2017/03  [Not refereed][Not invited]
  
• 海外情報 第17回アジア太平洋畜産学会議の概要とアジアオセアニア地域における畜産研究の動向

  後藤 貴文  畜産技術 = Livestock technology  (741)  34  -38  2017/02  [Not refereed][Not invited]
  
• Application of Metabolic Programming to Beef Production

  後藤 貴文  アグリバイオ = Agricultural biotechnology  1-  (2)  194  -202  2017/02  [Not refereed][Not invited]
  
• 高等大型哺乳類における初期成長期の栄養水準の差異は成熟期にどのような違いをもたらすのか？

  後藤 貴文  DOHaD研究  6-  (1)  60  -60  2017  [Not refereed][Not invited]
  
• Application of Metabolic Programming to Feeding Animal and Disease Prevention

  後藤 貴文  家畜感染症学会誌 = The journal of farm animal in infectious disease  6-  (2)  57  -63  2017  [Not refereed][Not invited]
   

  近年、胎児期や生後の初期成長期に受けた栄養刺激により、その後の動物体の代謝システム、体質および形態、さらに最近の研究では、特に肝臓、骨格筋および脂肪組織の代謝に多大な影響を及ぼすことが明らかになりつつある。近年、実験動物を用いた研究が医学分野で進んでいる。これはDOHaD(Developmental Origins of Health and Disease:成長過程の栄養状態や環境因子の作用に起因する疾患の発生)という概念として医学分野で捉えられ、エピジェネティクス研究分野と関連して代謝プログラミングあるいは代謝インプリンティングとも呼ばれる。近年、医学分野においてDOHaD概念に関連してヒトの疫学調査やモデル動物実験が盛んにおこなわれている。特に妊娠期の栄養と免疫刺激あるいは感染症が、子孫の代謝や表現型にどのような影響を及ぼすのかについての研究報告が多数集まりつつある。また、それらの相互作用についても考慮される。本講演では、DOHaDに関連した研究の動向について紹介し、代謝プログラミングシステムの家畜への応用の可能性とその研究の必要性について議論する。(著者抄録)
• Potential of Application of Metabolic Programming for Beef Production

  後藤 貴文  アミノ酸研究 = Amino acid research  11-  (1)  29  -36  2017  [Not refereed][Not invited]
  
• 田中畜産の放牧敬産牛の哲学 : 黒毛和牛但馬牛の繁殖経営と放牧敬産牛肉の販売

  後藤 貴文  畜産の情報 = Livestock industries information  (326)  43  -50  2016/12  [Not refereed][Not invited]
  
• 和牛の代謝プログラミングと肉質制御(その2)

  後藤 貴文  畜産技術 = Livestock technology  (738)  12  -16  2016/11  [Not refereed][Not invited]
  
• 和牛の代謝プログラミングと肉質制御(その1)

  後藤 貴文  畜産技術 = Livestock technology  (737)  2  -5  2016/10  [Not refereed][Not invited]
  
• 代謝プログラミングとその畜産への応用の可能性

  後藤 貴文  日本畜産学会大会講演要旨集  121回-  109  -109  2016/03  [Not refereed][Not invited]
  
• 麦焼酎粕濃縮液と稲WCSを混合した発酵飼料の黒毛和種仔牛への給与が骨格筋タンパク質分解関連遺伝子発現に及ぼす影響

  鶴岡 克彦, 倉原 貴美, 酒井 奏, 阿部 菜奈子, 日高 康志, 高橋 秀之, 後藤 貴文  日本畜産学会大会講演要旨集  121回-  154  -154  2016/03  [Not refereed][Not invited]
  
• 黒毛和種とホルスタイン種における濃厚飼料と粗飼料の給与パターンが体重と筋内脂肪含量に及ぼす影響

  後藤 貴文, 高橋 秀之, 塩塚 雄二, 藤野 亮一, 衛藤 哲次  日本畜産学会大会講演要旨集  121回-  156  -156  2016/03  [Not refereed][Not invited]
  
• Potential of IoT in livestock production:Production System of Kyushu University Brand Beef QBeef

  GOTOH Takafumi  The Proceedings of Mechanical Engineering Congress, Japan  2016-  (0)  K13200  2016  [Not refereed][Not invited]
   

  There are many serious problems in the beef production of Japan. The focus of this paper will be basically in four parts: 1) current problems of beef production in Japan, 2) Analysis of the metabolic programming effect in Wagyu: to create a fatty constitution in calf to produce good quality beef from grass foraging, and 3) to design a new beef production system utilizing grass resources on mountainous area. I accomplished Kyushu University Brand "Q Beef" cattle four years ago. To fatten Q Beef cattle by grazing on the mountainous area, 4) we created an ICT system to monitor the feeding of grazing cattle by smartphone, comprising the following elements: (1) actuators, including an audio player, feeder, and stanchion(an equipment for locking cattle neck during feeding ); (2) wireless nodes with Wi-Fi connectivity to control the actuators; (3) Internet protocol (IP)-addressed cameras with Wi-Fi connectivity; (4) a wireless access point enabling the wireless nodes and IP cameras to connect to a wired network using Wi-Fi; and (5) a Web server that communicates with the wireless nodes and delivers a control Graphical User Interface (GUI) for use by farmers. Farmers can call cattle towards an IP camera and feed them by using a smartphone without being physically present on the farm. Next, we constructed a received signal strength (RSS)-based location system for grazing cattle. This included monitoring the location of cattle on pastures in mountainous areas. To fatten Q Beef cattle on the mountainous area, we created two systems controllable via a smartphone, one system to call cattle and the other to monitor their locations. We introduce other devices which support farmers. These systems could be very useful to farmers in controlling and monitoring cattle grazing.

• アメリカの牛肉生産を学ぶ学生研修プログラムの挑戦 : カンザス州立大学におけるプログラム

  後藤 貴文, 廣政 恭明, 岡本 正宏  畜産の研究  69-  (10)  873  -880  2015/10  [Not refereed][Not invited]
  
• Co–Culture with Amniotic Membrane Stem Cells for Oocyte Maturation and Early Embryonic Development in Bovine

  Han Kil–Woo, Chun Ju Lan, Lee Ji Hey, Kim Keun–Jung, Lee Kyung-Bon, Gotoh Takafumi, Goto Takafumi, Do Chang Hee, Kim Min Kyu  九州大学大学院農学研究院紀要  60-  (2)  393  -398  2015/09/18  

  To improve early embryonic development, many strategies have been studied in various species including bovine. The co–culture of embryos with somatic cells has been known to improve embryo quality during in vitro culture and pregnancy rates compared with conventional culture systems. The co–culture of embryos with oviductal cells which have been used for blastocyst expansion appears to shorten the time needed for synthesis of hCG, early embryo development, and hatching blastocysts. The aim of this study is to investigate whether the co–culture system with amniotic membrane stem cells (bAMS) improves bovine embryonic development competence. In order to investigate the influence of bAMS on early embryo development, bAMS originated from fetal bovine amniotic membranes were used as the feeder layer during in vitro maturation. The rates of matured oocytes, cleavage division, and blastocysts formation were increased (p<0.05) after co–culture with the bAMS compared to the control group. Moreover the rates of oocyte maturation and embryo development were significantly higher with using bAMS below 5 passages. In addition, the GSH level and the total cell number of blastocysts were significantly higher in co–culture group than in those of control group. Apoptotic cells were found more in the control group, and the expression of anti–apoptosis related genes such as Mcl–1 and Bcl–2 were increased after co–culture with bAMS. In conclusion, the present study demonstrated that co–culture with bAMS cells enhances oocyte maturation and embryo developmental competence by increasing GSH level and expression of anti–apoptotic genes with reducing apoptotic cells in bovine embryos. We also found that it is more suitable to use bAMS below 5 passages.
• Effects of Dam Age, Lamb Gender, and Singleton or Twin Status on Bodyweight and Chest Measurements of Malya Lambs in Middle Anatolia, Turkey

  Gotoh Takafumi, Goto Takafumi  九州大学大学院農学研究院紀要  60-  (2)  371  -375  2015/09/18  

  We investigated the effects of dam age, lamb gender, and singleton or twin status on lamb chest measurements and body weight in Malya lambs in Middle Anatolia, Turkey. Data were obtained from 302 lambs in an elite Malya sheep flock. Bodyweight, chest circumference, chest depth, chest length, and chest width were recorded. The least square means method was used to determine factors that significantly affected lamb body weight and chest measurements. The general linear model in the Minitab packet program was used to test the effects of variables on body weight and chest measurements. Dam age did not significantly affect lamb bodyweight or chest measurements during the first year of life, except for chest circumference and chest depth. Male lambs were significantly larger in bodyweight and chest measurements than females at the age of 12 months (p<0.001 for all parameters). Singleton lambs were significantly larger in bodyweight and chest–related parameters than twins until 4 months of age, but this effect was only significant at 12 months of age for chest width. It is suggested that there might be compensatory growth in twin lambs after weaning. If farmers are able to ensure twin lamb survival until weaning age, they can expect compensatory growth by 12 months of age.
• 黒毛和種牛の初乳におけるメタボローム解析の検討

  西浦 誠, 高橋 秀之, 矢野 純司, 衛藤 哲次, 塩塚 雄二, 藤野 亮一, 後藤 貴文, 舟場 正幸, 松井 徹, 友永 省三  日本畜産学会大会講演要旨集  120回-  51  -51  2015/09  [Not refereed][Not invited]
  
• ウシ骨格筋中微量元素含量と筋線維型の関係

  松井 徹, 後藤 貴文, 船津 祥一郎, 友永 省三, 舟場 正幸  日本畜産学会大会講演要旨集  120回-  93  -93  2015/09  [Not refereed][Not invited]
  
• Expressional regulation of chemerin and its receptors in the liver and adipose tissues of young cattle by weaning and nutrition.

  Yutaka Suzuki, Daichi Kato, Mitsuhiko Kondo, Hizuru Hatanaka, Satoshi Haga, Takafumi Gotoh, Sanggun Roh  ADSA-ASAS JAM 2015  588  2015/07  [Refereed][Not invited]
  
• 黒毛和種仔牛における哺乳量の差が初期成長期の成長と代謝に及ぼす影響

  松原 敦子, 高橋 秀之, 木村 優里, 斎藤 昭, 野村 碧, Sithyphone Khounsaknalath, 藤野 亮一, 塩塚 雄二, 衛藤 哲次, 古瀬 充宏, 後藤 貴文  日本畜産学会大会講演要旨集  119回-  138  -138  2015/03  [Not refereed][Not invited]
  
• 麦焼酎粕濃縮液の黒毛和種仔牛への給与が発育、ルーメン内液性状、および血液性状に及ぼす影響

  鶴岡 克彦, 倉原 貴美, 酒井 奏, 阿部 菜奈子, 三輪 友樹, 日高 康志, 高橋 秀之, 後藤 貴文  日本畜産学会大会講演要旨集  119回-  138  -138  2015/03  [Not refereed][Not invited]
  
• 黒毛和種仔牛における哺乳期栄養の違いが初期成長期の栄養素代謝に及ぼす影響

  高橋 秀之, 松原 敦子, 木村 優里, 齋藤 昭, 野村 碧, Sithyphone Khounsaknalath, 藤野 亮一, 塩塚 雄二, 衛藤 哲次, 古瀬 充宏, 後藤 貴文  日本畜産学会大会講演要旨集  119回-  183  -183  2015/03  [Not refereed][Not invited]
  
• 去勢牛の肝臓メタボロームに及ぼすGLP-1とグレリンの影響

  杉野 利久, El-Sabagh Mabrouk, 谷口 大, 小櫃 剛人, 高橋 秀之, 後藤 貴文, 谷口 幸三  日本畜産学会大会講演要旨集  119回-  221  -221  2015/03  [Not refereed][Not invited]
  
• 赤身和牛肉の魅力をいかに伝えるか : レストランを基盤として生産者と消費者をつなぐ、きたやま南山の挑戦 (特集 国産畜産物の需要拡大に向けて)

  後藤 貴文  畜産の情報 = Livestock industries information  (304)  13  -20  2015/02  [Not refereed][Not invited]
  
• A106 Relationship of blood flow and contact pressure in cow

  Nagatomo Yasutaka, Inoue Ryo, Nogami Hirohumi, Goto Takahumi, Sawada Renshi  The Proceedings of the JSME Conference on Frontiers in Bioengineering  2015-  (0)  11  -12  2015  [Not refereed][Not invited]
  
• 動物用光MEMSセンサにおける波長特性の追究

  井ノ上 涼, 永友 康貴, 野上 大史, 後藤 貴文, 澤田 廉士  精密工学会学術講演会講演論文集  2015-  (0)  809  -810  2015  [Not refereed][Not invited]
   

  皮膚の色や厚みによって光は散乱・吸収・透過の特性が大きく異なる。牛に利用する光MEMSセンサ（脈波センサ・血流量センサ）において、最適な波長を選択するために波長特性を追求した。
• 耕作放棄地を活用した放牧による肉用牛生産の取り組み : 岡山県高梁市の日本短角種を用いた地域の取り組み

  後藤 貴文  畜産の情報 = Livestock industries information  (295)  41  -47  2014/05  [Not refereed][Not invited]
  
• 粗飼料肥育の黒毛和種における初期栄養が筋内細胞外マトリックスに与える影響

  野村 碧, 齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 塩塚 雄二, 藤村 亮佑, 衛藤 哲次, 高橋 秀之, 後藤 貴文  日本畜産学会大会講演要旨集  118回-  151  -151  2014/03  [Not refereed][Not invited]
  
• 牛肉の筋線維タイプと遊離アミノ酸量の関係性

  岡 佳輝, 水野谷 航, 後藤 貴文, 友永 省三, 中村 真子, 辰巳 隆一, 池内 義秀  日本畜産学会大会講演要旨集  118回-  219  -219  2014/03  [Not refereed][Not invited]
  
• Growth and Productivity Based on Metabolic Imprinting in Calves

  後藤 貴文  家畜感染症学会誌 = The journal of farm animal in infectious disease  3-  (4)  129  -138  2014  [Not refereed][Not invited]
   

  近年、輸入飼料に過度に依存している日本の牛肉生産はBSE(牛海綿状脳症)等の発生に見られる食の安全性に関する問題、集約的経営形態から排出される大量の糞尿処理問題、それに関わる環境問題、集約的な飼養形態における家畜福祉等の多くの問題を抱える。ウシは本来、家畜としてヒトが消化できない植物中の粗い繊維質(繊維性の高い通常の動物では消化できない植物多糖資源)を分解し、草資源からタンパク質源としての食肉を生産し、それをヒトに供給するという重要な物資循環機能を担った反芻家畜(草食動物)である。言い換えるとセルロース等からタンパク源を生産するコンバーター的アニマルと言える。一方、近年、胎子期や生後の初期成長期に受けた栄養刺激により、その後の動物体の代謝システム、体質および形態、さらに最近の研究では、特に肝臓、骨格筋および脂肪組織の代謝に多大な影響を及ぼすことが明らかになりつつある。近年、実験動物を用いた研究が医学分野で進んでいる。これはDOHaD(Developmental Origins of Health and Disease:成長過程の栄養状態や環境因子の作用に起因する疾患の発生)という概念として医学分野で捉えられ、エピジェネティクス研究分野と関連して代謝プログラミングあるいは代謝インプリンティングとも呼ばれる。著者らは、これまでの黒毛和種に関する研究により脂肪交雑の高さ、骨格筋の組織化学的特徴を確認しており、黒毛和種のユニークな能力を示してきた。現在、輸入濃厚飼料に過度に依存した黒毛和種の飼養システムを新しい生物科学的コンセプトを導入して革新し、現在の牛肉生産の問題点を解決したい。そのために、黒毛和種の飼養を、輸入穀物飼料多給ではなく、本来の草食獣の飼養形態を基盤として、代謝インプリンティングを組み合わせることで、粗飼料(牧草等、植物資源)を基盤とした肥育システムへシフトしたいと考えている。本総説では、代謝インプリンティングに関する研究の背景と著者らの取り組みを紹介しながら、子牛の成長と産肉性、さらには、新しい牛肉生産システムの可能性を論じる。(著者抄録)
• Search!! 研究室Report 学術の最先端はいま! ICTを活用した牛放牧における遠隔地管理システム

  後藤 貴文, 村西 明  月刊LASDEC : 地方自治情報誌  43-  (11)  30  -35  2013/11  [Not refereed][Not invited]
  
• 日本の森と山を活用する林畜複合経営の可能性 : 生物学的基礎、土地の集積、省力･効率化技術そしてマーケティングが必要 (特集 放牧のさまざまな効用を考える : 低コスト・省力化・高付加価値・地域貢献などで再評価)

  後藤 貴文  畜産コンサルタント  49-  (11)  41  -47  2013/11  [Not refereed][Not invited]
  
• Reconstruction of Phylogenetic Relationships of Korean Chickens Using Mitochondrial D–loop Sequences

  Hoque Md. Rashedul, Seo Dong–Won, Jo Cheorun, Gotoh Takafumi, Lee Jun–Heon, Goto Takafumi  九州大学大学院農学研究院紀要  58-  (2)  287  -293  2013/09  [Not refereed][Not invited]
   

  Two hypotheses of monophyletic and multiple origins have been documented for chicken domestication. The maternally inherited D–loop region in mtDNA is widely used for the investigation of relationships between populations and individuals of various animal species. In this study, 854 Korean chicken mtDNA D-loop sequences were investigated. Thirty five nucleotide substitutions were identified from the sequence analyses, and they were classified as twenty eight haplotypes. Phylogenetic analyses revealed five divergent mtDNA clades (A to E) based on geographical distribution. There was no breed specific clade among the Korean chickens. Moreover, the estimation of network profiles was traced using 28 haplotypes and five mtDNA clades. Korean chickens were discriminated based on the nucleotide positions. The clades A, B, and E are distributed universally in Eurasia, and among them, 95.78% Korean chickens were included. On the other hand, C and D clades were represented 2.34 and 1.87% of Korean chickens, respectively. Our mtDNA results indicate that Korean chickens share mtDNA haplotypes with a number of chicken breeds from South China, Japan, Europe, Middle East and Indian subcontinent regions. Therefore, the results suggest that Korean chickens have multiple maternal origins.
• 頭脳循環を加速する若手研究者戦略的海外派遣プログラム(3)ドイツライプニッツ家畜生物学研究所およびミュンヘン工科大学を訪問して

  後藤 貴文, 中村 真子, 柴田 昌宏  畜産の研究  67-  (9)  907  -912  2013/09  [Not refereed][Not invited]
  
• 同様の飼料を給与した黒毛和種とホルスタイン種の成長に伴う血漿アミノ酸の変化

  古瀬 充宏, 池田 裕美, Elke Albrecht, Steffen Maak, Jochen Wegner, 塩塚 雄二, 衛藤 哲次, 後藤 貴文  日本畜産学会大会講演要旨集  117回-  83  -83  2013/09  [Not refereed][Not invited]
  
• 頭脳循環を加速する若手研究者戦略的海外派遣プログラム(2)タイチェンマイ大学を訪問して

  後藤 貴文, 中村 真子, 柴田 昌宏  畜産の研究  67-  (5)  559  -566  2013/05  [Not refereed][Not invited]
  
• B-1-42 Propagation measurements between a subcutaneous implant and body surface of cattle for ICT aided grazing

  Yokoo Kaoru, Ishikawa Shouhei, Nishidoi Takeshi, Sugiyama Jun, Gotoh Takafumi, Tan aka Kenji  Proceedings of the IEICE General Conference  2013-  (1)  42  -42  2013/03/05  [Not refereed][Not invited]
  
• 初期成長期の高栄養処理が黒毛和種去勢雄牛の粗飼料肥育における胸最長筋での糖代謝関連遺伝子群の発現に及ぼす影響

  藤村 亮佑, 齋藤 邦彦, 阿部 剛, 金田 修一, 長谷部 浩行, 衛藤 哲次, 塩塚 雄二, 後藤 貴文  日本畜産学会大会講演要旨集  116回-  138  -138  2013/03  [Not refereed][Not invited]
  
• 黒毛和種における初期成長期栄養と筋内細胞外マトリックスの関係に関する研究

  野村 碧, 齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 塩塚 雄二, 藤村 亮佑, 衛藤 哲次, 高橋 秀之, 後藤 貴文  日本畜産学会大会講演要旨集  116回-  180  -180  2013/03  [Not refereed][Not invited]
  
• 哺乳期の給与飼料の違いが黒毛和種仔牛の成長および胸最長筋における栄養素代謝に及ぼす影響

  高橋 秀之, 木村 優里, 齋藤 昭, 猪内 勝利, 秋月 亮介, 野村 碧, 藤村 亮佑, 塩塚 雄二, 衛藤 哲次, 後藤 貴文  日本畜産学会大会講演要旨集  116回-  180  -180  2013/03  [Not refereed][Not invited]
  
• 哺乳期の給与飼料の違いが黒毛和種仔牛の胸最長筋における脂肪形成に関わる遺伝子群の発現への影響

  木村 優里, 高橋 秀之, 齋藤 昭, 猪内 勝利, 秋月 亮介, 野村 碧, 藤村 亮佑, 塩塚 雄二, 衛藤 哲次, 後藤 貴文  日本畜産学会大会講演要旨集  116回-  181  -181  2013/03  [Not refereed][Not invited]
  
• 黒毛和種仔牛における哺育形態の違いが肝の免疫および成長系因子の発現に与える影響

  秋月 亮介, 木村 優里, 齋藤 昭, 猪内 勝利, 野村 碧, 藤村 亮佑, 塩塚 雄二, 衛藤 哲次, 高橋 秀之, 後藤 貴文  日本畜産学会大会講演要旨集  116回-  181  -181  2013/03  [Not refereed][Not invited]
  
• ウシ培養肝細胞におけるChemerinの発現と調節因子

  北山 峻, Ardiyanti Astrid, 山内 恵利, 後藤 貴文, 萩野 顕彦, 加藤 和雄, 盧 尚建  日本畜産学会大会講演要旨集  116回-  186  -186  2013/03  [Not refereed][Not invited]
  
• 自給飼料活用における耕作放棄地放牧の可能性をさぐる : 肉用牛の放牧飼養と管理技術に関する取り組み (特集 自給飼料生産の振興に向けて)

  後藤 貴文  畜産の情報  (280)  5  -16  2013/02  [Not refereed][Not invited]
  
• 放牧や粗飼料を主体とした牛肉生産の可能性

  後藤 貴文, 衛藤 哲二, 塩塚 雄二  食肉の科学  54-  (2)  147  -153  2013  [Not refereed][Not invited]
  
• 頭脳循環を加速する若手研究者戦略的海外派遣プログラム(1)ドイツライプニッツ家畜生物学研究所を訪問して

  後藤 貴文, 中村 真子, 柴田 昌宏  畜産の研究  66-  (5)  505  -509  2012/05  [Not refereed][Not invited]
  
• 黒毛和牛における飼養システムの比較 : 代謝インプリンティングと国内の草資源を活用した新しい生産システム構築への挑戦

  後藤 貴文  畜産技術  0-  (683)  20  -26  2012/04  [Not refereed][Not invited]
  
• Grass-fed Wagyu (Japanese Black) beef production by Metabolic Imprinting on Nutrition manipulation and Utilization of domestic grass resources

  藤田 達男, 阿比留 真吾, 木下 正徳, 松田 謙一郎, 後藤 貴文  大分県農林水産研究指導センター研究報告. Bulletin of Oita Prefectural Agriculture, Forestry and Fisheries Research Center  (2)  4  -10  2012/03  [Not refereed][Not invited]
   

  黒毛和種雄牛12頭を用いて、哺育期に炭水化物を強化した代用乳および人工乳給与後、育成期を濃厚飼料給与区と乾草のみ給与区に分け、さらに肥育期には両区を畜舎内乾草給与区と放牧区の計4区に分けて約30ヵ月齢まで飼育した。7～23ヵ月齢の体重は、育成期に濃厚飼料を給与した区が有意に高く、育成期の濃厚飼料給与は発育に強く影響することが示唆された。枝肉格付け等級はA2:2頭、B2:4頭、B1:6頭であったが、育成期に濃厚飼料給与した区の方が枝肉重量、ロース芯面積、バラの厚さおよびBMSNoが高い傾向がみられ、育成期の濃厚飼料給与は発育と肉質の双方を高める効果が示唆された。部内職員を対象として食味試験した結果、予想以上に柔らかく、あっさりして美味しかったといった好意的な評価が多かった。経済性を試算した結果、育成期に濃厚飼料を多給し代謝的インプリンティングを誘導した後に放牧肥育を行う方法が最も収益性が高かった。
• 黒毛和種の優良種雄牛クローン子牛の初期成長期における高栄養処理が胸最長筋の遺伝子発現に及ぼす影響

  藤村 亮佑, 藤田 達男, 阿比留 真吾, 衛藤 哲次, 塩塚 雄二, 江原 史雄, 松田 謙一郎, 盧 尚建, 西村 正太郎, 後藤 貴文  日本畜産学会大会講演要旨集  115回-  145  -145  2012/03  [Not refereed][Not invited]
  
• 黒毛和種哺乳雄子牛における代用乳の給与量と質の違いが成長および生理状態に及ぼす影響

  寺尾 裕美, 阿比留 真吾, 木下 正徳, 齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 齋藤 昭, 塩塚 雄二, 松田 謙一郎, 藤村 亮佑, 衛藤 哲次, 後藤 貴文  日本畜産学会大会講演要旨集  115回-  226  -226  2012/03  [Not refereed][Not invited]
  
• Metabolic Imprinting Effect in Beef Production: Effects of Nutrition Manipulation during an Early Growth Stage on Fatty Acid Composition in Longissimus Muscles of Wagyu (Japanese Black)

  Takafumi Gotoh, Kotaro Etoh, Kunihiko Saitoh, Kaoru Saitoh, Hironori Sakuma, Kaori Sakuma, Syuichi Kaneda, Tsuyoshi Abe, Tetsuji Etoh, Yuji Shiotsuka, Kenichiro Matsuda, Hidetoshi Suzuki, Hiroyuki Hasebe, Fumio Ebara, Akira Saitoh, Jochen Wegner  JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE  2-  S88  -S88  2011/09  [Not refereed][Not invited]
  
• The Influence of Fattening by Eco-Feed Based on Okara on the Growth, Meat Quality and Histochemical Properties of the Longissimus Thoracis Muscle in Japanese Black Cattle

  Khounsaknalath Sithphone, Matsuda Ken'ichiro, Shiotsuka Yuji, Etoh Tetsuji, Fumita Tomiko, Shiroshita Takashi, Sin Takeshi, Gotoh Takafumi  九州大学大学院農学研究院紀要  55-  (2)  247  -252  2010/10/29  [Not refereed][Not invited]
   

  The objective of this article is (1) to examine effects of eco-feed on histochemical properties in Longissimus thoracis muscle, (2) to identify the effectiveness of eco-feed utilization in composition of fattening concentrate and (3) to investigate quality of eco-feed beef taste. After 10 months reared, ten animals were divided into convention (steers, n=6) and eco-feed (steers, n=2; heifers, n=2) group, and fattened in similar feeding system based on numbers in each group. In eco-feed group, fattening concentrate was removed approximately 40 percent and replaced by eco-feed for slaughtered at age of 26 months and then all beef quality was evaluated. Samples from around 6th thoracis in Longissimus muscle were performed in histochemical techniques. Quality of test was confirmed by the taste panel test from French and Italian restaurant in Fukuoka. The results showed that the body growth and quality of beef in both groups did not differ (P<0.05) in all categories. The composition of myofibre type I of steers (40%) in conventional group was larger than steers (25%) and heifers (29%) in eco-feed group (P<0.01). The size of myofibre type I had no significantly different for both steers and heifers between groups and the diameter type I, type IIA and type IIB in convention group was almost the same size, while in eco-feed the size of myofibre type IIA and IIB were larger than those in convention (P<0.01) group. The taste of eco-feed beef was extremely impressed by beef steak consumers at high confirmation level. As a result, eco-feed had no negative effect on the animal growth and its beef taste. The quality of beef of eco-feed were almost the same with common fattened; histochemical properties confirmed a slightly better in eco-feed animals in terms of beef quality grades, scores and levels.
• Metabolic imprinting effects in grass-fed Wagyu (Japanese Black) beef production

  Takafumi Gotoh  FASEB JOURNAL  24-  2010/04  [Not refereed][Not invited]
  
• 初期成長期の代謝生理的インプリンティング効果の解明 黒毛和種への強化哺乳が粗飼料肥育牛の枝肉成績に及ぼす影響

  齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 小山田 幸夫, 金田 修一, 長谷部 浩行, 斎藤 昭, 衛藤 浩太郎, 江原 史雄, 衛藤 哲次, 塩塚 雄二, 後藤 貴文  日本畜産学会大会講演要旨集  112回-  108  -108  2010/03  [Not refereed][Not invited]
  
• 初期成長期の代謝生理的インプリンティング効果の解明 牛肉生産に関与した因子群の粗飼料肥育終了時の動態解析

  後藤 貴文, 衛藤 浩太郎, 齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 小山田 幸夫, 長谷部 浩行, 齋藤 昭, 塩塚 雄二, 江原 史雄, 衛藤 哲次  日本畜産学会大会講演要旨集  112回-  109  -109  2010/03  [Not refereed][Not invited]
  
• Character of Oribatid mite composition in Japanese grassland with description of a new species

  中村 好男, 中村 好徳, 橋本 新一, 後藤 貴文  Science bulletin of the Faculty of Agriculture, Kyushu University  64-  (2)  109  -118  2009/10  [Not refereed][Not invited]
   

  In the Bahiagrass pasture, the number of oribatid species was smaller than in the chestnut orchard under eco-friendly agriculture and the grassy lawn in the park. Four of eleven species collected from the Bahiagrass pasture were also collected from the chestnut orchard and the grassy lawn. The remaining seven species were peculiar in the Bahiagrass pasture. A new species, Hermannia (Phyllhermannia) maruokaensis, was collected from the grassy lawn. Oribatid mites are subdivided taxonomically into Lower, Brachypylina and Poronata. In Japanese grassland and pasture, the number of Lower (primitive) species was abundant. On the other hand, the abundant species was the Brachypylina species in the forest and the Poronata species in the cultivated field.
• 初期成長期の代謝生理的インプリンティング効果の解明 黒毛和種子牛への強化哺乳が粗飼料肥育期の発育に及ぼす影響

  齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 小山田 幸夫, 金田 修一, 齋藤 昭, 衛藤 浩太郎, 江原 史雄, 衛藤 哲次, 塩塚 雄二, 後藤 貴文  日本畜産学会大会講演要旨集  111回-  60  -60  2009/09  [Not refereed][Not invited]
  
• 初期成長期の代謝生理的インプリンティング効果の解明 牛肉生産に関与した因子群の動態解析

  後藤 貴文, 衛藤 浩太郎, 齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 小山田 幸夫, 齋藤 昭, 塩塚 雄二, 江原 史雄, 衛藤 哲次  日本畜産学会大会講演要旨集  111回-  60  -60  2009/09  [Not refereed][Not invited]
  
• 初期成長期の代謝生理的インプリンティング効果の解明 第一胃内性状および細菌叢に及ぼす影響

  小池 聡, 齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 小山田 幸夫, 齋藤 昭, 衛藤 浩太郎, 江原 史雄, 衛藤 哲次, 塩塚 雄二, 後藤 貴文  日本畜産学会大会講演要旨集  111回-  60  -60  2009/09  [Not refereed][Not invited]
  
• 哺乳期に摂取する代用乳の量および質の差異が黒毛和種雄子牛の生理形態に与える影響

  松田 謙一郎, 阿比留 真吾, 木下 正徳, 衛藤 浩太郎, 齋藤 邦彦, 佐久間 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 小山田 幸夫, 齋藤 昭, 塩塚 雄二, 江原 史雄, 衛藤 哲次, 後藤 貴文  日本畜産学会大会講演要旨集  111回-  61  -61  2009/09  [Not refereed][Not invited]
  
• 交雑種去勢雄牛における初期成長期の給与飼料の違いが胸最長筋のグルコース代謝に及ぼす影響

  盛川 美和子, 江原 史雄, 稲田 淳, 浅田 研一, 磯崎 良寛, 齋藤 昭, 後藤 貴文  日本畜産学会大会講演要旨集  111回-  71  -71  2009/09  [Not refereed][Not invited]
  
• ウシ初期成長期の代謝生理的インプリンティング効果の解明 育成期における牛肉生産に関与した遺伝子群の動態解析

  衛藤 浩太郎, 衛藤 哲次, 江原 史雄, 中村 好徳, 目時 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 斉藤 昭, 後藤 貴文  日本畜産学会大会講演要旨集  110回-  102  -102  2009/03  [Not refereed][Not invited]
  
• A Simple Method of Optical Evaluation of the Distribution of Muscle Extracellular Matrix by Immunofluorescence and Image Analysis using Fluorescent Semiconductor Nanocrystals (quantum dot)

  Nakamura Yoshi-Nori, Fumita Tomiko, Hayashi Keisuke, Ebara Fumio, Etoh Tetsuji, Shiotsuka Yuji, Hattori Masa-aki, Gotoh Takafumi  九州大学大学院農学研究院紀要  54-  (1)  103  -108  2009/02/27  [Not refereed][Not invited]
   

  A simple method using optically stable quantum dots (Qdots) for immunohistochemical (IHC) analysis was tested to investigate their signal localization and stability in extracellular matrix (ECM) components of bovine longissimus thoracis muscles in fattened Japanese black steers. Detection of primary antibodies of each ECM component in IHC was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide in Qdots with a fluorescence microscope (FM). We could observe each ECM component by IHC using Qdots in a FM for a long time. Each ECM component was detected clearly in the perimysium and endomysium in skeletal muscle. Using computer image analysis software, each ECM component was reconstructed in detail: structures were clear, with the distribution of each component of the ECM shown after 3D conversion based on the strength of fluorescenece. It is possible and useful to apply a simple method of IHC using Qdots under FM to the detailed investigation of ECM components in skeletal muscle.
• Comparative Ultrastructural Observation of the Cuticle and Muscle of an Enchytraeid (Enchytraeus japonensis) and an Oribatid species (Tectocepheus velatus) using Transmission Electron Microscopy

  Yoshi-Nori Nakamura, Takafumi Gotoh  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY  54-  (1)  97  -101  2009/02  [Not refereed][Not invited]
   

  We observed internal tissue of wet soil mesoanimal, Enchytraeus japonensis and dry soil meso animal, Tectocepheus velatus using optical and transmission electron microscopes. Thin cuticle layer and thick epidermis covered the whole body, and there were epidermal gland cells of various forms in the cuticle of E. japonensis. the epidermis was formed of the bilayer, and thick body wall muscles of various travels were observed in the hypodermic. There were many lipid droplets (large and small size) under body wall muscles. On the other hand, the mite body T. velatus was surrounded by thick sclerites. The Sclerites formed a thin epidermis (outer layer), a thick epicuticle (middle layer) and a thick procuticle (inner layer). The procuticle was formed of 5-6 thin layers. However, there was the position with the impossible discriminate in other place. The hypodermic muscles were connected to the procuticle, and were surrounded by many lipid dorplets. There were lipid droplets (large and small sizes) in various places. This study indicates that there are internal histological differences of soil animals according to different habitat, and environment in soil.
• 「日本産肉研究会 第3回学術集会」開催される--九州における赤肉生産の戦略

  後藤 貴文  畜産コンサルタント  45-  (2)  60  -65  2009/02  [Not refereed][Not invited]
  
• Study of availability of seabuckthorn strained lees as cattle feed

  SHIOTSUKA Yuji, SANO Motonobu, NAKAMURA Yoshinori, ETOH Tetsuji, FUMITA Tomiko, HAYASHI Keisuke, GOTOH Takafumi  Science bulletin of the Faculty of Agriculture, Kyushu University  63-  (1)  35  -39  2008/02  [Not refereed][Not invited]
   

  Seabuckthorn （Hippophae rhamnoides L.） is the plant which contained many nutrients, for vitamin. In this study, the effect of seabuckthorn lees （SL） which added in concentrate feed on the growth and blood parameter, in particular factors in relation to stress in the weaning period was investigated using Japanese black cattle （n＝15）. Control group （n＝5, body weight: 81.1±4.6kg） , 5％ SL group （n＝5, 85.4±9.3kg） and 10 ％ SL group （n＝5, 80.2±6.4kg） were set up. The changes of body weight and blood parameter from wean to six months cattle were investigated. Body weight in 5 ％ and 10 ％ SL group were lower （P＜0.05） than that of control group. There were differences of serum total protein, albumen and triglyceride among group. Contents of γ-GTP in 5％ group was higher （P＜0.05） those of control and 10 ％ group from weaning to one month. The contents of IgA, IgG and IgM were not changed. Therefore growth and blood parameter in cattle were not affected by the addition to SL （within 10 ％）. However, body weight in cattle decreased for SL addition. For use of SL, future study needs to investigate the processing and extracting of SL.
• A Novel Feeding System Mainly Utilized by Domestic Grass Resources and Grazing in Beef Cattle(Contribution of Animal and Grassland Research for Solution of Problem in Food Supply and Environmental Conservation)

  Gotoh Takafumi, Etoh Tetsuji, Shiotsuka Yuji, Hayashi Keisuke, Fumita Tomiko  Japanese Journal of Grassland Science  54-  (2)  182  -194  2008  [Not refereed][Not invited]
   

  食料・環境問題に対する畜産・草地研究の貢献。放牧を軸とした国内草資源フル活用による新たな肉牛飼養システムの提案。近年、地球レベルの環境問題、世界的な異常気象、食料問題、政治不安、国際間の経済協定に伴う貿易関係、国際間の戦争、テロ対策等に関する戦争の長期化と世界の状況は様々な面で混沌とした時代に突入している。食料生産に関しても、地球に住む我々は、これまでの生産性重視の時代から、上記に関わる種々の要因を総合的に考慮し、長期的な視点にたった施策や技術の開発が望まれる。このような状況を踏まえ農業、特に畜産の分野においても、これまでの比較的縦割りであった学問領域を超えた"学際的な"研究の推進が必要である。今後、農学研究者は、どのような方向に進むべきかを慎重に議論し、明確なビジョンあるいは日本独自の農業哲学を構築し、明確な目標を設定する必要がある。それを実現するために、これまでの科学的知見を集積、再構築し、これまでにない持続型ならびに環境保全型の新しい技術の開発による新しい農業の創出が望まれる。我々は、ウシの体質制御と国内の草資源を活用した安全で良質な牛肉の新しい生産システムの構築を目指して模索し、研究を行っている。この新しい肉牛飼養システムの構築に向けての研究について、これまでの成果を紹介するとともに、本システムを提案したい。
• Comparative observations on the growth changes of the histochemical property and collagen architecture of the Musculus pectoralis from Silky, layer-type and meat-type cockerels

  Ichiro Oshima, Hisao Iwamoto, Shoji Tabata, Yoshitaka Ono, Akira Ishibashi, Nobuya Shiba, Hideyuki Miyachi, Takafumi Gotoh, Shotaro Nishimura  ANIMAL SCIENCE JOURNAL  78-  (6)  619  -630  2007/12  [Not refereed][Not invited]
   

  Growth-related changes in the histochemical properties and collagen architecture of the Musculus pectoralis were compared among Silky, layer-type and meat-type cockerels. Histochemical and immunohistochemical methods were used and collagen architecture was studied using scanning electron microscopy. The total amount of collagen present was also measured. The diameter of type IIB myofibers was similar or rather larger in the layer-type birds compared with the meat-type. The collagen content was generally low for 5-10 weeks across the breeds and then increased in the other breeds except for Silky. In the perimysium, the collagen bundles gradually increased in size and the density of the fibrils also increased during growth. At 30 weeks of age, the layer-type birds showed compact collagen bundles while the meat-type had loose bundles. The endomysial collagen network appeared relatively denser in the meat-type chicks compared to the others at week 1. At 30 weeks of age, compact and felt-like structure of endomysium was shown by Silky and layer-type chickens, while the meat-type showed a relatively loose arrangement of tissue in the endomysial collagen. From these results, it appears that the meat-type chicken can produce a large M. pectoralis with many, relatively thinner myofibers and a relatively undeveloped form of intramuscular collagen structure.
• 肉牛:代謝生理的刷り込みを活用した草資源フル活用型新飼養技術 (特集 家畜栄養管理技術の最近の進歩)

  後藤 貴文, 高橋 博人  畜産技術  (629)  9  -16  2007/10  [Not refereed][Not invited]
  
• 代謝生理的インプリンティング効果の解明 高蛋白低脂肪代用乳の多給が交雑種雄哺育牛の発育性に及ぼす影響

  稲田 淳, 浅岡 壮平, 浅田 研一, 磯崎 良寛, 江原 史雄, 齋藤 昭, 後藤 貴文  日本畜産学会大会講演要旨集  108回-  52  -52  2007/09  [Not refereed][Not invited]
  
• 黒毛和種子牛への強化哺乳が胸最長筋内の筋制御因子の発現に及ぼす影響

  衛藤 浩太郎, 後藤 貴文, 衛藤 哲次, 中村 好徳, 江原 史雄, 目時 香織, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 金田 修一, 鈴木 英敏, 斉藤 昭  日本畜産学会大会講演要旨集  108回-  57  -57  2007/09  [Not refereed][Not invited]
  
• 初期成長期の代謝生理的インプリンティング効果の解明 黒毛和種子牛への強化哺乳が発育に及ぼす影響

  目時 香織, 衛藤 浩太郎, 増田 恭久, 岡田 真人, 阿部 剛, 小林 栄治, 鈴木 英敏, 金田 修一, 斎藤 昭, 江原 史雄, 衛藤 哲次, 中村 好徳, 後藤 貴文  日本畜産学会大会講演要旨集  108回-  57  -57  2007/09  [Not refereed][Not invited]
  
• 和牛における初期成長期の代謝生理的インプリンティング効果の解明 発育に関する研究

  江原 史雄, 文田 登美子, 中村 好徳, 林 恵介, 衛藤 哲次, 塩塚 雄二, 岩元 久雄, Wegner Jochen, 後藤 貴文  日本畜産学会大会講演要旨集  107回-  99  -99  2007/03  [Not refereed][Not invited]
  
• 和牛における初期成長期の代謝生理的インプリンティング効果の解明 骨格筋の形態学的特質に関する研究

  中村 好徳, 文田 登美子, 江原 史雄, 衛藤 哲次, 塩塚 雄二, 岩元 久雄, Wegner Jochen, 後藤 貴文  日本畜産学会大会講演要旨集  107回-  99  -99  2007/03  [Not refereed][Not invited]
  
• 和牛における初期成長期の代謝生理的インプリンティング効果の解明 筋分化制御因子及び筋成長因子に関する研究

  後藤 貴文, 文田 登美子, 中村 好徳, 江原 史雄, 衛藤 哲次, 塩塚 雄二, Wegner Jochen, 岩元 久雄  日本畜産学会大会講演要旨集  107回-  99  -99  2007/03  [Not refereed][Not invited]
  
• 和牛における初期成長期の代謝生理的インプリンティング効果の解明 脂肪細胞分化制御因子群に関する研究

  文田 登美子, 中村 好徳, 江原 史雄, 衛藤 哲次, 塩塚 雄二, 稲田 淳, 岩元 久雄, Wegner Jochen, 後藤 貴文  日本畜産学会大会講演要旨集  107回-  100  -100  2007/03  [Not refereed][Not invited]
  
• Comparison of nutritional composition and histological changes of the soybean seeds cultivated by conventional and organic farming systems after long-term storage - preliminary study.

  Nakamura.Y, Fujita.M, Nakamura.Y, Gotoh.T  Journal of the Faculty of Agriculture, Kyushu University  52-  (1)  1  -10  2007  [Not refereed][Not invited]
   

  We investigated the effect of hermetic long-term (9-year) storage at room temperature on the chemical compositions and histological structure of soybean seeds that had been cultivated by two farming systems [conventional farming (CF) or organic (natural) farming (OF)]. Soybean seeds had been harvested from plants cultivated in the field under CF practices, or in a neighboring field where OF practices had been used for over 3 years. Protein content, calcium (Ca), potassium (K) and phosphorus (P) content of OF soybean seeds were higher than CF seeds just after harvesting. However, after long-term storage, protein content of OF soybean seeds was significantly higher than CF seeds. In addition, there were many minerals with a higher content (in particular, K) of OF soybean seeds. The breaking strength and pH of OF soybean seeds were higher than CF ones. Optical microscopic examination revealed collapsed tissue and gaps in the internal and external parts of CF soybean seeds. Transmission electron microscopic photographs of CF soybean seeds confirmed the presence of collapsed cell walls and protein bodies. These results indicate that the type of farming system (inorganic or organic fertilizers) used results in differences in chemical compositions and histological structure of product during storage. In addition, our results indicated that high Ca content of OF soybean seeds strengthened cell wall structure, and prevented the loss of contents during storage.
• 乳用種去勢牛における育成期のTMR給与が発育性および骨格筋の産肉性関連遺伝子群の発現に及ぼす影響

  稲田 淳, 古賀 鉄也, 浅田 研一, 磯崎 良寛, 文田 登美子, 後藤 貴文  日本畜産学会大会講演要旨集  106回-  26  -26  2006/03  [Not refereed][Not invited]
  
• 代謝生理的インプリンティング機構の解明:牛初期成長期の給与飼料の違いが骨格筋中の肉質関連遺伝子発現に及ぼす影響

  文田 登美子, 田中 章子, 衛藤 哲次, 塩塚 雄二, 岩元 久雄, 後藤 貴文  日本畜産学会大会講演要旨集  106回-  121  -121  2006/03  [Not refereed][Not invited]
  
• 耕作放棄地に放牧した黒毛和牛の産肉的特性

  林 恵介, 文田 登美子, 衛藤 哲次, 稲田 淳, 後藤 貴文  日本畜産学会大会講演要旨集  106回-  128  -128  2006/03  [Not refereed][Not invited]
  
• 牛飼養における肉質に関連した代謝生理的インプリンティング効果の検討

  文田 登美子, 衛藤 哲次, 塩塚 雄二, 岩元 久雄, 後藤 貴文  日本畜産学会大会講演要旨集  104回-  191  -191  2005/03  [Not refereed][Not invited]
  
• Histochemical properties of skeletal muscles in Japanese cattle and their meat production ability

  Takafumi Gotoh  Animal Science Journal  74-  (5)  339  -354  2003/10  [Not refereed][Not invited]
   

  The compositional characteristics of the three basic types of myofiber, namely type I (slow-twitch oxidative), type IIA (fast-twitch oxidative glycolytic) and type IIB (fast-twitch glycolytic), are clarified in the skeletal muscles of Japanese Black cattle. The myofiber composition, which is characteristic of the muscles of Japanese Black cattle, markedly changes during their growth, when some type IIA myofibers are transformed into type I or IIB, depending on the different muscles. Independent of these changes with growth, inter- and intramuscular variations of myofiber type distribution is evident. The small extensor muscles in deep regions around bone contain a lot of type I myofibers, whereas the large muscles at surface regions have many type II myofibers. Japanese Black cattle have typical white muscles such as the Longissimus thoracis and Semitendinosus, containing half the myofibers as red (type I + IIA). The muscles of Japanese Black cattle show a tendency to contain a higher percentage of type I myofibers than other breeds over an intrabreed variation of the myofiber type composition. In the big muscles such as the Longissimus thoracis and Biceps femoris, a great diversity of myofiber type composition is observed among the different regions. When fattened, heifers produce Longissimus thoracis and Biceps femoris muscles of smaller weight than steers, but in heifers the myofiber size in each type is rather larger. In the Psoas major, Vastus lateralis and Serratus ventralis muscles, heifers contain a higher frequency of red (type I + IIA) myofibers with no differences in myofiber size. Among the several muscles of fattened Japanese Black steers, the percentage distribution of type I myofibers has a positive correlation with the percentage amount of intramuscular fat. From these results, the high potential of Japanese Black cattle to produce marbled beef could be based on the histochemical properties of myofibers in their skeletal muscles.
• Effects of Different Feed on Myostatin mRNA Expression in Skeletal Muscles of Japanese Black Cattle

  馬場 明子, 文田 登美子, 衛藤 哲次, ほか2名, 後藤 貴文  Bulletin of the Kyushu University Farm  (11)  11  -14  2003/03  [Not refereed][Not invited]
   

  Myostatin, a member of the transforming growth factor(TGF-β)-superfamily, have function as a negative regulator of skeletal muscles growth. We examined the effect of different feeds on myostatin mRNA expression in skeletal muscles of Japanese Black Cattle. The cattle were divided into two groups; one group(two cattle) was mainly fed with concentrate (group-C), another(two cattle) was mainly fed with roughage(group-R). The muscle materials were taken from M.biceps femoris (BF) and M.longissimus thoracis (LT) at 5 months and 7 months of age by needle biopsy method, and stored in liquid nitrogen. After total cellular RNA was extracted from those tissues by ISOGEN, the RT-PCR analysis was carried out. At 5 months of age, in both muscles, the intensity of expression level for myostatin mRNA in group-C was stronger than in group-R. At 7 months of age, in BF, the expression of myostatin mRNA in group-C was stronger than in group-R. However, in LT, the expression made no difference between 2 groups. The present study suggested that the expression of myostatin mRNA in these muscles of calves might be influenced by the feeding system.
• Expression of Peroxisome Proliferator-Activated Receptor gamma2(PPAR_γ2) mRNA in Muscle Tissues of Japanese Black Cattle

  文田 登美子, 馬場 明子, 渡邊 潤, ほか3名, 西村 光博, 後藤 貴文  Bulletin of the Kyushu University Farm  (11)  46  -50  2003/03  [Not refereed][Not invited]
   

  Peroxisome proliferator-activated receptor gamma2 (PPAR_γ2) is one of the nuclear receptors and is known as a main regulator of adipogenesis. In this study, we investigated the influence of the feeding system on the expression of PPAR_γ2 mRNA in muscle tissue of young Japanese Black cattle. Four animals were divided into two groups: one group was separated from the mother cows at 3 days and was raised by artificial nursing until 2 months of age. After that they were fed with high concentrate (group C). Another was raised and grazed with their mother cows until 3 months. After weaning, they were fed only with roughage (group R). Muscle tissues were taken from M. longissimus thoracis (LT) and M. biceps femoris (BF) by needle biopsy at 5 and 7 months of age. Total RNA was isolated with ISOGEN from these tissues. Using the total RNA, we carried out RT-PCR to measure the expression of PPAR_γ2 mRNA. At 5 months, high expressions of PPAR_γ2 mRNA were observed in LT and BF of group C. On the other hand, in group R, there were no or low expressions of PPAR_γ2 mRNA. At 7 months, in both groups, the expressions were observed and the expressions in group C were higher than those in group R. From these results, it was suggested that feeding system during calf might influence the expression of PPAR_γ2 mRNA in muscle tissue of Japanese Black cattle and be related to intramuscular adipogenesis.
• Interrelationship between myofiber type composition and intramuscular-fat content in muscles of Japanese Black cattle

  衛藤 哲次, 篠原 孝明, 文田 登美子, ほか5名, 阪下 邦仁, 大園 正陽, 岩元 久雄, 後藤 貴文  Bulletin of the Kyushu University Farm  (11)  51  -55  2003/03  [Not refereed][Not invited]
   

  The interrelationship between myofiber type composition and intramuscular-fat content was investigated in muscles of Japanese Black cattle. The cattle were mainly fed with concentrate and slaughtered at 26-29 mo of age. After slaughter, M.longissimus thracis, M.biceps femoris, M.semimembranosus, M.semitendinosus, M. vastus lateralis, M.serratus ventralis and M.rectus femoris were removed from each carcass. Myofibers were enzyme-histochemically classified into type I, IIA and IIB myofibers according to the nomenclature of Brooke and Kaiser(1970). The percentage of intramuscular-fat content in each muscle was measured with Soxhlet method. In these muscles, the percentage of each type myofiber was related significantly to the percentage of intramuscular-fat content of each muscle at 26-29 mo of age. Correlation coefficient between the percentage of Type I myofiber and intramuscular-fat content was positive significant (p<0.005). Conversely, correlation coefficient between the percentages of type IIA, IIB myofiber and intramuscular-fat content was negative significant (p<0.05). These results indicated that the percentage of Type I myofiber was significantly related to the accumulation of intramuscular-fat in the muscles of Japanese Black cattle.
• 3-14 樹園跡地植生の群落内照度に及ぼす異なる放牧強度の影響

  宮川 創, 林 恵介, 植田 彩, 塩塚 雄二, 衛藤 哲次, 重盛 進, 増田 泰久, 名田 陽一, 後藤 貴文  日本草地学会誌  49-  (0)  178  -179  2003  [Not refereed][Not invited]
  
• 3-15 放牧強度が異なる樹園跡地における植生の短期的変化

  林 恵介, 宮川 創, 植田 彩, 衛藤 哲次, 塩塚 雄二, 重盛 進, 増田 泰久, 名田 陽一, 後藤 貴文  日本草地学会誌  49-  (0)  180  -181  2003  [Not refereed][Not invited]
  
• Seasonal prevalence of Echinochloa crus-galli (L.) Beauv and Digitaria adscendens (H. B. K.) Henr. in meadows in the central highland area of Kyushu

  Ikeda K., Goto T., Etou T., Shiotuka Y., Nisimura T., Masuda Y.  Journal of Weed Science and Technology  48-  (0)  98  -99  2003  [Not refereed][Not invited]
  
• Grazing of Solidago altissima L. by Japanese Black Cattle in the grove abandoned

  Ueda A., Hayashi K., Miyagawa S., Eto T., Goto T., Masuda Y.  Journal of Weed Science and Technology  48-  (0)  104  -105  2003  [Not refereed][Not invited]
  
• Histochemical properties of skeletal muscles in Japanese cattle and their meat production ability

  Animal Science Journal  74:339-354-  2003  [Not refereed][Not invited]
  
• The Relationship between Meat Quality and Feeding System in Japanese Black Steers ; Can much concentrate feed during 3 to 14 months of age improve meat quality in the longissimus muscle of fattened Japanese steers?

  Proceeding of the 9th AAAP Animal Science Congress  B-  232  2000  [Not refereed][Not invited]
  
• Histochemical Properties of Skeletal Muscles in Different Body Parts of Young Japanese Black Steers

  GOTOH Takafumi, IWAMOTO Hisao, NAKANISHI Yoshitaka, UMETSU Raizaburo, ONO Yoshitaka  Animal science journal  70-  (6)  497  -509  1999/11/25
• Myofiber Type Distribution in the Cranial Portion of M. biceps femoris of Japanese Black Young Steers

  GOTOH Takafumi, IWAMOTO Hisao, NAKANISHI Yoshitaka, UMETSU Raizaburo, ONO Yoshitaka  Animal science journal  70-  (6)  510  -518  1999/11/25
• Comparative Study on the Myofiber Type Composition and the Fat Deposit in M. longissimus thoracis between young Heifer Groups of Japanese Black fed freely on Concentrate and Roughage

  GOTOH T  Proceeding of 45th International Congress of Meat Science and Technology  1-  278  -279  1999  [Not refereed][Not invited]
  
• Influence of Feed Quality on pulsatile release patterns of Growth Hormone in Young Japanese Black heifers

  Proceeding of 3rd Conference Farm Animal Endocrinology  33  1998  [Not refereed][Not invited]
  
• Relationship between social grooming and agonistic behavior in a stable fattening cattle herd.

  Nakanishi Yoshitaka, Shimizu Yoshimasa, Sumiyoshi Youichi, Kawamura Toshihisa, Goto Takafumi, Umetsu Raizaburo  Journal of the Faculty of Agriculture, Kyushu university  37-  (3-4)  325  -329  1993  [Not refereed][Not invited]
   

  Wheat starch-gluten mixture was converted into a bioplastics like material (bioplastics) under processing at an elevated temperature. The density of the bioplastics was 1.24 -1.26 g/cm^3 depending on the ratio of starch to gluten. The color difference of the bioplastics was altered with the starch-gluten ratio. Endothermic peak by differential scanning calorimetry was considerably decreased with the increase of the ratio of gluten to starch. It was revealed that the physical characteristics of the bioplastics were dependent on the starch-gluten ratio and that wheat starch and gluten interacts at their interface with the increase of the glass-transition temperature.
• Comparative Aspects of Behavioural Activities of Beef Cows before and after Introducing a Stranger at Night.

  Nakanishi,Y, Kawamura.T, Gotoh.T, Umetsu.R  Journal of the Faculty of Agriculture, Kyushu university  37-  (3-4)  227  -238  1993  [Not refereed][Not invited]
   

  Two groups of dry-lot feeding beef cows comprising 7 residents and a stranger (Groups A and B) were used to examine differences in social and maintenance behaviours before and after introducing the stranger at night, i.e. after dark when most resident animals were recumbent while resting or ruminating and there was less social activity. Agonistic and social licking interactions and incidents of eating, lying, standing and locomotion of each animal in each group were recorded during the daytime.' Body weight change for each cow in the groups after introduction was also estimated. In Group A, there was little change in the frequencies of agonistic and social licking behaviours during the observation period. In Group B, there was more than a 2-fold increase in the frequency of agonistic behaviour following the introduction, though social licking changed little over the period. In each group, a relatively higher frequency of aggressive behaviour initiated and received by each stranger occurred on the 1st to 2nd day after introduction, which thereafter tended to decrease until the 7th day. The stranger in Group A significantly received more frequent aggression from residents than a stranger in Group B (P < 0.05). The strange cow in each group spent less time eating and more time in locomotion than residents. A significant negative correlation between eating time and relative body weight change in Group A (P < 0.05) suggested that a greater weight reduction in the strange cow after introduction is mainly associated with her less eating time.

Awards & Honors

• 2021/09 Animal Production Science Animal Production Science's 60 years of publication (Animal Production Science in the 21st Century)
   (selected paper) Potential of the application of epigenetics in animal production (Takafumi Gotoh) 

  受賞者: Takafumi Gotoh
• 2020/02 Leave a Nest Co., Ltd., The Kagoshima Bank Ltd. Kagoshima Tech Planter, Award by Kagoshima Bank
   Big Revolution of Beef Production 

  受賞者: Takafumi Gotoh
• 2016/09 Leave a Nest Co., Ltd., Yammer Holdings Co.,Ltd. Awarded by Japanese Society of Animal Science for a thesis entitled “Histochemical Properties of Skeletal Muscles in Japanese Cattle and Their Meat Production Ability”.
   Package construction of Q Beef production 

  受賞者: Takafumi Gotoh
• 2001/03 Japanese Society of Animal Science Award of Japanese Society of Animal Science
   Meat production ability and histo-chemical properties in skeletal muscles of Wagyu 

  受賞者: Takafumi Gotoh

Research Grants & Projects

• 放牧基盤型飼養のためのIoTと宇宙技術に よる戦略的スマート畜産技術の開発

  生研支援センター：オープンイノベーション研究・実用化推進事業

  Date (from‐to) : 2023/07 -2029/03 

  Author : 後藤貴文, 内田義崇, 神武直彦, 小高暁, 慶應義塾, 中西波瑠, 株式会社神戸デジタル・ラボ, 松永圭史, 株式会社システムフォレスト
• The role of histone modification in bovine skeletal muscle altered by nutrition during fetal life

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2022/04 -2025/03 

  Author : 室谷 進, 後藤 貴文, 大島 一郎
• 令和3年度代謝刷り込みによる牛放牧肥育技術開発事業

  令和3年度 日本中央競馬会畜産振興事業

  Date (from‐to) : 2021 -2024/03 

  Author : 後藤貴文, 大島 一郎, 下桐 猛, 安尾 しのぶ, 川畑 健次, 室谷 進
• Revolution of Beef Production with Design-in system for Sustainable Utilization of Rural Nature Resource

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

  Date (from‐to) : 2019/07 -2022/03 

  Author : 後藤 貴文

   

  現在の畜産は、高騰する輸入飼料相場に翻弄される経営困難、循環不可の過剰糞尿処理、BSE等の食の安全、脂肪過多牛肉志向で硬直したマーケット、さらに集約的飼養による動物福祉等、多くの問題を抱えている。新しい生物科学概念「代謝プログラミング」研究をシーズとして牛の代謝を早期に制御し、飼料には日本 の豊富な植物資源を放牧により高度活用し、その飼養管理には先端ICTを駆使する。さらに販売には、エシカル（倫理的な）ダイレクトマーケットを構築し、若 い農業者が未来に希望をもち、産業として世界と戦えるよう畜産業を構造改革し大革新することを目標とした。今年度は以下のような結果を得た。①代謝プログ ラミング研究：遺伝子発現解析、microRNA発現解析、骨格筋の組織化学的解析、メタボローム解析、血清を用いた培養によるin vitro解析、およびDNAメチル化解析を進め、メカニズムの一部を明らかとした。②ICT放牧管理システム：放牧肥育牛の素牛を生産するためのシステムとして、耳標とは別のタグ画像解析による個体認識をベースとした遠隔個体給餌システムのしくみの基盤を再構築した。インプラント体温センサーによるウシの発情看視システムの仕組みを構築した。③マーケティング：放牧牛肉の硬さに関係するコラーゲンについて蛍光免疫組織による3次元的構築により基盤的知見を得た。以上、今年度も黒毛和種における「代謝プログラミング」に関して分子生物学的解析を進めて、そのメカニズムの一端を明らかとし、日本の植物資源における牛肉生産、いわゆるタンパク質生産の最適メカニズムの一端を構築した。耕作放棄での放牧飼養に適した先端ICTシステムを構築した。さらに、そこから生産される牧草牛肉の肉質に関する基盤的知見を得た。
• 過疎地活性化のための地球観測と高精度測位による放牧地と放牧牛をモニタ可能な自動飼養システムの構築

  文部科学省：宇宙利用技術創出プログラム

  Date (from‐to) : 2020/09 -2022/03 

  Author : 後藤貴文, 神武 直彦, 内田 義崇
• Investigation of microRNA functions in metabolic imprinting of Japanese Black cattle

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

  Date (from‐to) : 2018/04 -2021/03 

  Author : Muroya Susumu

   

  The pregnant cows were fed on low-nutrition (LN) or high-nutrition (HN) of their overall nutritional requirement during gestation. The longissimus thoracis muscle of Japanese Black fetal calves was analyzed by metabolomics and transcriptomics. Maternal undernutrition decreased the bodyweight and muscle weight of the fetus. The levels of amino acids (AAs) and arginine-related metabolites including glutamine were higher in the LN group than those in the HN group. Metabolite set enrichment analysis revealed that the highly different metabolites were associated with the metabolic pathways of pyrimidine, glutathione, and AAs, suggesting that MUN resulted in AA accumulation rather than protein accumulation. The expression of genes associated with energy metabolism, glycolysis, angiogenesis, and insulin signaling pathways were lower in the LN group than in the HN group.
• 令和2年度畜産・酪農生産力強化対策事業

  公益社団法人 畜産技術協会：令和2年度畜産・酪農生産力強化対策事業（繁殖性向上対策のうち繁殖性向上に資する情報の測定・分析技術の実証）

  Date (from‐to) : 2020/11 -2021/03 

  Author : 後藤 貴文
• AIやICTを活用した放牧管理システムの実装と基盤強化

  独立行政法人 農畜産業振興機構：令和2年度畜産関係学術研究委託調査

  Date (from‐to) : 2020/08 -2021/03
• ＩＣＴ等を活用した周年親子放牧による肉用牛子牛生産の省力化・低コスト化技術の開発.

  農林水産省：革新的技術開発事業・緊急展開事業、先導プロジェクト（包括提案型）

  Date (from‐to) : 2016 -2020
• 胎児期と初期成長期の代謝プログラミングによる和牛肥育期間の大幅な短縮技術の開発.

  農林水産省：革新的技術開発事業・緊急展開事業、先導プロジェクト（個別提案型）

  Date (from‐to) : 2016 -2020
• The Development of Novel Food CirculationSystem: Metabolic imprinting, the New Beef Production by Grass Resource and ICT

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

  Date (from‐to) : 2014/07 -2018/03 

  Author : GOTOH Takafumi, YABE Mitsuyasu

   

  We developed a metabolic imprinting and an information and communications technology (ICT, or Internet of Things) management system for Wagyu beef production as future systems.We partly found out the mechanism of metabolic imprinting by analysis of molecular biology in Wagyu cattle. finally, we produced safe, high-quality Wagyu beef using domestic pasture resources while solving the problems of how to utilize increasing areas of abandoned agricultural land and to make use of the plant-based feed resources in Japan’s mountainous areas.We also confirm the potential of grassfed beef market by Web questionnaire.
• Evolustion of Beef Production System

  一般財団法人 キヤノン財団：研究助成プログラム「理想の追求」

  Date (from‐to) : 2016 -2018 

  Author : Takafumi Gotoh
• Development of Cattle Production System by Metabolic Programming: Metabolic Regulation System during Fetus and Neonate

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

  Date (from‐to) : 2013/04 -2017/03 

  Author : GOTOH TAKAFUMI, TAKAHASHI Hideyuki, Albrecht Elke, MAAK Steffen, PFFAFL Michael

   

  Recent research has discovered a unique phenomenon referred to as ‘fetus and neonatal programming’, which is based on ‘the developmental origins of health and disease (DOHaD)’ concept. These studies have shown that alterations in fetus and early postnatal nutrition and endocrine status may result in developmental adaptations that permanently change the structure, physiology and metabolism of affected animals during adult life. The timing of the perturbation in maternal nutrient availability plays an important role in determining the effect that the fetal and neonatal programming have on the developing placenta or fetus and offspring performance. In this study about Japanese Black cattle, it was suggested that there might be strong effects of maternal and neonatal nutrition on the meat quality and quantity.
• 黒毛和牛における革新的強化哺育技術および飼養システムに関する研究

  全国酪農業協同組合連合会：

  Date (from‐to) : 2011/08 -2016/03
• Creation of implanted temperature sensor for grazing cattle by ICT

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

  Date (from‐to) : 2013/04 -2015/03 

  Author : GOTOH TAKAFUMI, HIRAYAMA Noritomo, UENO Hideo, IWASAKI Wataru

   

  The purpose of this project was to develop the vital sensing system for cattle, what is called, implanted temperature sensor/actuator, to realize to monitor cattle health, heat, calving and so on. If sensor attaches on the body of cattle, data of it is strongly affected by the environment. Therefore I utilized the small temperature sensor used in industries and modified it to adjust cattle body with collaboration with some companies. I succeeded to develop the model of implanted temperature sensor for cattle and could get data on the PC with wireless system from grazing cattle. I believe that this technology could contribute to improve or innovate cattle management.
• 肉用牛繁殖性向上緊急対策のうち繁殖性向上に資する

  公益社団法人 畜産技術協会：平成26年度畜産・酪農生産力強化緊急対策事業

  Date (from‐to) : 2015 -2015
• 焼酎粕とイネ発酵粗飼料を活用した豊後牛生産のための新規ブランド育成飼料の開発.

  株式会社アグリサポートあじむ：

  Date (from‐to) : 2012/08 -2014/08
• 若手研究者による革新的家畜生産学術領域の構築と戦略的国際ネットワークの形成.

  日本学術振興会：頭脳循環を加速する若手研究者戦略的海外派遣プログラム

  Date (from‐to) : 2011 -2013
• センサーNWを活用したICT農業に関する研究

  NTT西日本株式会社：

  Date (from‐to) : 2012 -2012
• 人に優しくスマートな放牧管理を実現する無線生体管理システムの研究開発.

  総務省：戦略的情報通信研究開発推進制度

  Date (from‐to) : 2011 -2012
• ICT技術を活用した高度放牧管理システムの研究・開発

  富士通株式会社：

  Date (from‐to) : 2011 -2012
• グラスフェッド型の黒毛和牛肉に関する市場調査

  フレンチレストラン ラ・プートウリーエ―：

  Date (from‐to) : 2011 -2011
• 「先端センシング技術による牛の人工授精適期検地システム」に関する共同研究

  ＮＳマテリアルズ株式会社：

  Date (from‐to) : 2010 -2010
• Characterization of molecular mechanisms of bovine fat accumulation by global gene expression analysis

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

  Date (from‐to) : 2008 -2010 

  Author : ROH Sanggun, GOTOH Takfumi, CHIKUNI Koichi, HIRAMATSU Kohzi

   

  This study was to investigate the molecular mechanism of fat accumulation and adipogenesis by DNA chip using adipose tissues from 10- and 24-month-old Japanese Black Cattle. Several candidate genes were separated and identified from global gene expression analysis. Adipogenin, chemerin and mPARM-1 genes were confirmed to relate to lipid metabolism and adipocyte differentiation in bovine adipocytes. These results suggested that several genes were involved with adipogenesis and fat accumulation in bovine.
• The Creation of Next Feeding System in Cattle : Studies on Metabolic Imprinting during Early Growth Stage

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

  Date (from‐to) : 2008 -2010 

  Author : GOTOH Takafumi

   

  In this study, we investigated how the metabolic imprinting during an early growth stage influences on constitution of Wagyu cattle by molecular-biologically, morphologically and physiologically investigating. In conclusion, it was clarified that the feeding at high energy level during the early growth stage, what is called, metabolic imprinting treatment, markedly influenced not only mRNA expressions of genes related to meat quantity and quality in skeletal muscles but also meat quantity and quality in Wagyu cattle.
• 乳酸菌二重カプセル剤バイタルフィードが強化哺乳時の消化吸収機構および免疫システムに与える影響に関する検討

  レイジントレードコーポレーション株式会社：

  Date (from‐to) : 2009 -2009
• 海藻由来製剤アルギカーフが強化哺乳時の消化吸収機構および免疫システムに与える影響に関する検討

  神協産業株式会社：

  Date (from‐to) : 2009 -2009
• 母牛の体液電気抵抗常時計測研究（先端ＩＴ技術を用いた繁殖雌牛の発情モニタリングシステムの構築）

  九州航空株式会社：

  Date (from‐to) : 2009 -2009
• 肉用牛における早期肥育に適した哺育・育成技術の開発

  長崎県：

  Date (from‐to) : 2008 -2009
• 代謝生理的刷り込みを活用した交雑種粗飼料多給型肥育技術

  福岡県：ふくおか型良質牛肉生産技術確立普及事業

  Date (from‐to) : 2008 -2009
• 納豆菌由来分解酵素およびキノコ類有用カビ類生物菌を活用した植物系および食品系廃棄物の機能性飼料化に関する研究開発

  公益社団法人 中央畜産会：

  Date (from‐to) : 2008 -2009
• Molecular comparison of extreme phenotypes (Japanese Black vs Charolais vs. Holstein) to understand molecular mechanisms of adipogenesis in cattle.

  ファイザーアニマルヘルス株式会社：

  Date (from‐to) : 2006 -2009
• 工場排熱と酵素による食品残さの飼料化に関する研究開発事業.

  株式会社アジア環境研究所：

  Date (from‐to) : 2006 -2009
• 草資源から安心・安全な高機能良質牛肉を生産する次世代型牛の選抜育種マーカーの探索

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Exploratory Research

  Date (from‐to) : 2005 -2006 

  Author : 後藤 貴文

   

  草資源による良質な牛肉生産は一般に難しいと考えられている。反芻類家畜の栄養摂取機構ではルーメンでの微生物による粗飼料中の繊維性植物多糖の分解に時間を要する。濃厚飼料多給に比べ粗飼料の栄養吸収は緩慢で効率が悪い。しかし、わが国の環境問題や消費者への家畜を通じたBSE等伝染病流入の危険性を考えるとわが国の草資源による安心・安全な新奇牛肉生産システムの開発が急務である。このようなシステムの構築にはこれまでの輸入穀物飼料による飼養に適した牛を育種するのではなく、草資源からの栄養吸収に優れた、あるいは草嗜好性の高い牛を選抜育種していくことが必要不可欠である。放牧形態により飼養されている繁殖牛は、通常痩せているが、その中には草のみの飼養にもかかわらず、かなり肥満している個体も見られる。このような個体がどのような栄養生理的および産肉生理的な特徴を有しているのかを探ることは、草資源による牛肉生産システム開発のためには必要不可欠であり、非常に興味深いモデルである。本研究課題では、放牧により肥満する牛群と痩身牛群について、まず血液サンプルについてインスリン様成長因子、ストレスホルモン等の解析を行ったが、それらの牛群間に有意な差異は認められなかった。また、バイオプシー筋サンプルを用いて、脂肪細胞分化制御因子群の発現を解析したが、体格のよい個体で遺伝子発現レベルが高い傾向が認められた。
• Construction of Novel Beef Production System : study of the effect of metabolic imprinting during the early growth period

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

  Date (from‐to) : 2003 -2006 

  Author : GOTOH Takafumi, HATTORI Massaki, MASUDA Yasuhisa

   

  Wagyu (Japanese Black cattle) are known for their excellent marbled beef by using a considerable amount of imported grain feed. We would like to apply effects of metabolic imprinting system to beef production : we would like to realize a good quality production system that by regulating the constitution of cattle by metabolic imprinting and feeding domestic grass resources can produce safe beef in Japan without using imported feed. We investigated the effects of metabolic imprinting during the early growth period on meat quality by comparing between two groups of Wagyu steers; group R was fed only roughage (hay) until slaughter (26 mo), while the other group C was fed concentrate (grain) from 2 to 10 mo of age and then fed only roughage until slaughter. We took muscle samples by biopsy during growth. Gene expressions related to intramuscular adipogenesis and muscle development were investigated by real-time PCR. The average live weight was 2.2-fold higher in group C (265±18 kg) than in group R (117±12 kg) just after 10 mo of age (p<0.001). At slaughter, group C was just 1.3-fold heavier (472±42 kg) than group R (357±25 kg) (p<0.001). In longissimus muscles, there were significant differences of the mRNA expressions of meat quality-and quantity related genes during growth between these two groups. In conclusion, the feeding level during the early growth stage influenced mRNA expressions in skeletal muscle. The growth size, meat quantity and quality were markedly different between groups. This may be caused by the effect of metabolic imprinting induced by a high feeding level during the early growth stage.
• 荒廃した農地跡地を利用した牛肉生産システムの構築

  Date (from‐to) : 2001 -2006
• 牛初期成長期の代謝的インプリンテイング効果の解明

  Date (from‐to) : 2001 -2006
• 頭部形成に働く遺伝子群に関する分子生物学的解析.

  公益財団法人ホクト生物科学振興財団：助成金

  Date (from‐to) : 2000 -2000
• 給与飼料の質の変化が和牛成長ホルモンの分泌パターンに及ぼす影響

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Encouragement of Young Scientists (A)

  Date (from‐to) : 1999 -2000 

  Author : 後藤 貴文

   

  成長ホルモン(Growth Hormone:GH)は体長の増加やタンパク質合成の促進、脂肪の分解、糖利用の低下などに影響を及ぼし、腺性下垂体より放出されるホルモンである。これまでに和牛(黒毛和種)を用いた実験により濃厚飼料を多給すると粗飼料を多給したときよりも著しくGHの分泌が減少し、そのパターンも著しく異なることを確認した。本研究では和牛の成長時期により給与飼料の質を変化させて、それによって血中GHの分泌パターンがどのように変化するにかを明らかにすることを目的として行った。昨年度は離乳前(3カ月齢時)から徐々に給与飼料を変化させる過程で採血を行い、飼料中の濃厚飼料の割合が増すにつれGHの分泌が著しく減少することを明らかにした。その後、これらの牛は8カ月齢まで濃厚飼料多給で飼養された。本年度は、これらの牛を用いて8カ月齢時より逆に給与濃厚飼料を徐々に減少させる(給与粗飼料の割合を高めていく)過程で採血を行いGH分泌パターンの変化を追求した。給与濃厚飼料を体重比3%,2.5%,2%,1.5%とそれぞれの変化に2週間かけて徐々に減少させながら経時的に採血した。採血方法は一時的に牛頚静脈にカテーテルを装着し、8時間15分間隔の採血を行い、血中より血清を分離し分析までメデイカルフリーザーで保存した。現在RadioimmunoassayによりGH concentrationを定量中である。
• 和牛の肉質に関する分子生物学的および形態学的解析
• 骨格筋の生体機構学的研究
• Influence of feed quality on pulsatile release patterns of growth hormone in Japanese Black cattle
• Functional Anatomy of Muscles

Educational Activities

Committee Membership

• 2022/08 -2022/08   68th International Congress of Meat Science and Technology (ICoMST) 組織運営員、学術委員、プログラム委員

Social Contribution

Social Contribution

Media Coverage

• 「放牧」次世代へ畜産の再構築を

  Date : 2023/10/18

  Program, newspaper magazine: 農業共済新聞

  第一面掲載 Paper
• “スマート放牧”で効率的な畜産を

  Date : 2023/09/01

  Publisher, broadcasting station: NHK

  Program, newspaper magazine: おはBiz

  Media report
• 放牧でおいしい牛肉を～佐賀・鹿島～

  Date : 2023/07/06

  Publisher, broadcasting station: NHK

  Program, newspaper magazine: ロクいち！福岡 食イチ！

  Media report
• 和牛放牧のススメ-肥育で実証進むさし入り可能に

  Date : 2023/02/15

  Writer: Myself

  Program, newspaper magazine: 日本農業新聞

  Paper
• “宇宙牛プロジェクト”で一石二鳥って？

  Date : 2022/07/20

  Publisher, broadcasting station: NHK

  Program, newspaper magazine: NHK NEWS WEB ビジネス特集

  Internet
• 鹿児島1次産業DX 宇宙牛プロジェクトで変わる畜産

  Date : 2022/06/24

  Publisher, broadcasting station: NHK鹿児島

  Program, newspaper magazine: かごしまWEB特集

  Internet