Researcher basic information

• 博士（獣医学）, 北海道大学

• https://researchmap.jp/keitarou.morisihta?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201301090364518340
• http://kaken.nii.ac.jp/d/r/30637046.ja.html

• 201301090364518340

• 貧血
• 臨床獣医学

• Life Science, Veterinary medical science

• Bachelor's degree program, School of Veterinary Medicine
• Doctoral (PhD) degree program, Graduate School of Veterinary Medicine

Research activity information

• Feb. 2020, 臨床研究アワード（インターズー学術奨励賞）非再生性貧血のミニチュア・ダックスフンド11例に対する脾臓摘出術の治療成績 第16回日本獣医内科学アカデミー学術大会（JCVIM2020））
  菅原芽伊;森下啓太郎, 今井 健友, 山崎 淳平, 佐々木 東, 大田 寛, 細谷 謙次, 滝口 満喜
• Aug. 2019, 令和元年度 北海道地区学会北海道獣医師会長賞
  細田 千尋, 森下 啓太郎, 山崎 淳平, 高田 健介, 細谷 謙次, 金 尚昊, 佐々木 東, 大田 寛, 滝口 満喜
• Aug. 2018, 平成30年度 北海道地区学会奨励賞
  岩永 優斗, 森下 啓太郎, 細谷 謙次, 大菅 辰幸, 佐々木 東, 大田 寛, 滝口 満喜
• Sep. 2014, 平成26年度 日本小動物獣医学会地区学会長賞
  森下啓太郎

• Evaluation of the course of improvement with molnupiravir treatment for feline infectious peritonitis.
  Shino Yoshida; Mei Sugawara-Suda; Kazuyoshi Sasaoka; Noboru Sasaki; Nozomu Yokoyama; Kensuke Nakamura; Keitaro Morishita; Sangho Kim; Takafumi Sunaga; Mitsuyoshi Takiguchi
  The Canadian veterinary journal = La revue veterinaire canadienne, 66, 5, 546, 554, May 2025, [International Magazine]
  English, Scientific journal, OBJECTIVE: To clarify the clinical course during molnupiravir treatment for feline infectious peritonitis (FIP). ANIMALS AND PROCEDURE: Cats diagnosed with FIP and treated with molnupiravir at Hokkaido University Veterinary Teaching Hospital (Sapporo, Hokkaido, Japan) were retrospectively reviewed. RESULTS: Eleven cats were eligible for inclusion. Six cats had effusive FIP and 5 had non-effusive FIP. In noneffusive cases, 2 cats had neurological abnormalities at diagnosis, whereas 1 additional cat developed neurological signs during treatment. The median initial dosage of molnupiravir was 13.0 mg/kg (range: 10.0 to 15.0 mg/kg), PO, q12h. One cat died after 11 d and the remaining 10 cats completed an 84-day course of treatment. All neurological cases were given dosage increases, extended treatment duration, or both. The median final dosage of molnupiravir in non-neuro-FIP cases was 13.1 mg/kg (range: 10.0 to 15.0 mg/kg), PO, q12h, whereas dosages in neuro-FIP cases were 15.0, 15.2, and 17.2 mg/kg, PO, q12h in the 3 affected cats, respectively. In non-neurological cases, dysrexia, lethargy, and high serum amyloid A were resolved within 15 d. Total follow-up duration ranged from 175 to 362 d. No relapses were observed. CONCLUSION AND CLINICAL RELEVANCE: Monitoring responses to molnupiravir treatment requires observing clinical signs and conducting clinicopathological evaluations, including acute-phase protein evaluation.
• ラブラドール・レトリーバーのコロニーで集団発生したサルファ剤過敏症とCYPB5Rの遺伝子型頻度解析
  岸原 果子; 横山 望; 山崎 淳平; 笹岡 一慶; 森下 啓太郎; 中村 健介; 宮原 和郎; 近藤 厚; 滝口 満喜; 高橋 徹
  北海道獣医師会雑誌, 68, 8, 314, 314, (公社)北海道獣医師会, Aug. 2024
  Japanese
• 慢性リンパ球性白血病により大細胞性リンパ腫がマスクされていた犬の1例
  塩原 希; 森下 啓太郎; 五日市 大介; 後藤 裕子; 富安 博隆; 滝口 満喜
  北海道獣医師会雑誌, 68, 8, 317, 317, (公社)北海道獣医師会, Aug. 2024
  Japanese
• Case report: Echinococcus multilocularis infection in a dog showing gastrointestinal signs in Hokkaido, Japan
  Izumi Kida; Naoki Hayashi; Nozomu Yokoyama; Noriyuki Nagata; Kazuyoshi Sasaoka; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Hirokazu Kouguchi; Kinpei Yagi; Ryo Nakao; Mitsuyoshi Takiguchi; Nariaki Nonaka
  Frontiers in Veterinary Science, 11, 1373035, 1373035, Frontiers Media SA, 13 Jun. 2024, [International Magazine]
  English, Scientific journal, Echinococcus multilocularis is a cestode that causes human alveolar echinococcosis, a lethal zoonotic disease distributed in the northern hemisphere. The life cycle of this parasite is maintained in nature by voles as intermediate hosts and foxes as definitive hosts in Hokkaido, Japan. Although dogs are also susceptible to the parasite, the infection has been considered typically asymptomatic. We report the detection of E. multilocularis eggs in the diarrheal feces of a dog with chronic gastrointestinal signs, which disappeared after anthelmintic treatment. The mitochondrial genome sequence constructed by sequencing of the overlapping PCRs using DNA from the eggs was identical to the most predominant haplotype previously reported in red foxes in Hokkaido. This case highlights that Echinococcus infection should be considered as a differential diagnosis for diarrheal dogs in the disease endemic areas. Further efforts are needed to accumulate parasite genotypes in domestic dogs as well as humans to assess the risk of human infection from dogs.
• Urinary steroid profiling using liquid chromatography-tandem mass spectrometry for the diagnosis of canine Cushing's syndrome.
  N Nagata; H Sawamura; Y Ikenaka; K Morishita; K Hosoya; N Sasaki; K Nakamura; M Takiguchi
  Veterinary journal (London, England : 1997), 106151, 106151, 29 May 2024, [International Magazine]
  English, Scientific journal, Serum cortisol measurements by chemiluminescence enzyme immunoassay (CLEIA) are widely used to diagnose hypercortisolism (HC) or Cushing's syndrome in dogs. However, they are associated with problems such as the need for multiple blood collections under stressful conditions or cross-reactivity between hormones. Therefore, a less invasive and more accurate diagnostic method is required. This study aimed to develop a urinary steroid profile analysis method using liquid chromatography-tandem mass spectrometry (LC/MS/MS) and to evaluate its clinical usefulness. Sixty-five healthy dogs and 38 dogs with suspected HC were included in the study. Using LC/MS/MS, the levels of 11 steroid hormones in the urine were determined. We established the upper limit of the reference interval for each urinary steroid-to-creatinine ratio and evaluated their diagnostic performances. The levels of the five steroid hormones were significantly higher in the 14 dogs with HC than in the 24 dogs with mimicking HC and 65 healthy dogs. The urinary corticosterone-to-creatinine ratio showed the highest diagnostic accuracy (area under the curve, 0.96). A significant correlation was seen between urinary cortisol concentrations measured by LC/MS/MS and CLEIA (rs = 0.88, P <0.001), although the CLEIA measurements were significantly higher than the LC/MS/MS measurements (P <0.001). LC/MS/MS-based urinary steroid profiles are a promising tool for diagnosing canine HC.
• Antimicrobial resistance and self-reported hand hygiene awareness before and after an infection prevention and control programme: a 7-year analysis in a small animal veterinary teaching hospital
  K. Sasaoka; T. Sato; K. Morishita; K. Hosoya; N. Yokoyama; T. Sato; M. Horiuchi; M. Takiguchi
  The Veterinary Journal, 306, 106154, 106154, Elsevier BV, May 2024, [International Magazine]
  English, Scientific journal, Infection prevention and control (IPC) in veterinary medicine is crucial to protect patients, owners, staff, and the public. An IPC programme is recommended for every animal hospital. The objective of this retrospective longitudinal study was to describe the changes in bacterial and multidrug-resistant (MDR) bacterial isolates and self-reported hand hygiene awareness and practices after an IPC programme to assess the long-term effect of this programme in small animal veterinary medicine. The IPC programme was implemented at our veterinary teaching hospital in April 2018, which included the establishment of an infection control task force, regular IPC lectures and poster campaigns, infrastructure improvement, and manual refinement. Laboratory-based surveillance was retrospectively conducted before and after the programme (January 2016-December 2022). Level and slope changes in bacterial isolates were evaluated using interrupted time-series analysis. Self-reported hand hygiene awareness and practices were assessed using an annual questionnaire. Additionally, hygiene product purchases during the study period were investigated. The monthly number of total and MDR bacterial isolates decreased significantly after the programme (MDR level change: -0.426; 95% confidence interval: -0.744, -0.109; P = 0.009; and MDR slope change: -0.035; 95% confidence interval: -0.058, -0.011; P = 0.003). Additionally, awareness of hand hygiene before touching animals improved after the programme. Overall self-reported hand hygiene practices improved, and hygiene product purchases significantly increased. These results suggested that the IPC programme may have long-term effects regarding reducing total and MDR bacterial isolates and improving hand hygiene awareness in veterinary medicine.
• 標準的感染症対策プログラムは動物病院において手指衛生コンプライアンス向上と多剤耐性菌出現抑制に貢献する
  笹岡 一慶; 森下 啓太郎; 細谷 謙次; 佐藤 敬近; 佐藤 豊孝; 堀内 基広; 滝口 満喜
  日本獣医学会学術集会講演要旨集, 166回, 174, 174, (公社)日本獣医学会, Sep. 2023
  Japanese
• 犬の腎うっ血の評価におけるShear wave elastographyの有用性の検討
  塩原 希; 中村 健介; 横山 望; 森下 啓太郎; 滝口 満喜
  北海道獣医師会雑誌, 67, 8, 316, 316, (公社)北海道獣医師会, Aug. 2023
  Japanese
• Successful treatment of sclerosing encapsulating peritonitis in a cat using bioresorbable hyaluronate-carboxymethylcellulose membrane after surgical adhesiolysis and long-term prednisolone
  Nozomu Yokoyama; Ryohei Kinoshita; Hiroshi Ohta; Kazuki Okada; Genya Shimbo; Kazuyoshi Sasaoka; Noriyuki Nagata; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Yumiko Kagawa; Mitsuyoshi Takiguchi
  Journal of Feline Medicine and Surgery Open Reports, 9, 2, 20551169231209917, 20551169231209917, SAGE Publications, Jul. 2023, [International Magazine]
  English, Scientific journal, Case summary
  
  An 8-year-old neutered male domestic shorthair indoor cat was presented with an 8-week history of intermittent vomiting, anorexia and weight loss that had been unresponsive to supportive treatment. Abdominal ultrasound revealed plication of the small intestine and fluid accumulation proximal to the lesion, and a linear foreign body was suspected. An exploratory celiotomy showed cocoon-like encapsulation of the entire intestine. Surgical adhesiolysis and full-thickness biopsy were performed, and histopathologic examination revealed mild thickening of the visceral peritoneum with fibrin deposition, as well as mild neutrophil and lymphocyte infiltration. These findings were compatible with sclerosing encapsulating peritonitis (SEP). The cat recovered well postoperatively and was discharged the next day. Prednisolone was administered for 7 weeks to prevent recurrence of SEP. Five months after surgery, the cat was re-presented with anorexia and chronic vomiting. Based on the clinical examination findings, recurrent SEP was suspected. At the second surgery, surgical adhesiolysis was repeated and a bioresorbable hyaluronate-carboxymethylcellulose membrane was used to cover the serosal surface and thus prevent adhesion formation. Histopathologic findings of the peritoneal biopsy specimen confirmed SEP. Long-term prednisolone treatment (1 mg/kg for the first dose and 0.5 mg/kg every 48 h for maintenance) was administered postoperatively. The cat survived for more than 1239 days without recurrence.
  
  Relevance and novel information
  
  To our knowledge, this is the first report of SEP in a cat with long-term survival. The use of a bioresorbable hyaluronate-carboxymethylcellulose membrane and long-term prednisolone treatment may have prevented short-term and long-term recurrence, respectively, in this case.
• Investigation of the therapeutic effects, predictors, and complications of long-term immunosuppressive therapy in dogs with precursor-targeted immune-mediated anemia.
  Mei Sugawara-Suda; Keitaro Morishita; Yuto Iwanaga; Jumpei Yamazaki; Yumiko Kagawa; Nozomu Yokoyama; Noboru Sasaki; Hiroshi Ohta; Kensuke Nakamura; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 85, 7, 695, 701, 01 Jul. 2023, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, Dogs with precursor-targeted immune-mediated anemia (PIMA) are commonly treated with immunosuppressive therapy, but information on predictors of treatment response and response time is limited. Therefore, we retrospectively investigated predictive factors that influenced the treatment response and duration required to observe a response in dogs with PIMA receiving continuous immunosuppressive therapies for more than 105 days. Of 50 client-owned dogs that developed PIMA, 27 were included in this study, of which 18 were responders and 9 were non-responders to immunosuppressive therapies. Sixteen of the 18 responders responded to treatment within 60 days and the remaining 2 responded at 93 and 126 days, respectively. We found that an erythroid-maturation ratio of <0.17 may be a useful predictor for treatment response. In addition, complications of immunosuppressive therapies were investigated further in 50 dogs. Pancreatitis (n=4) and pneumonia (3) occurred over the entire treatment period, and infections such as abscesses (3) tended to be more common in dogs on an extended period of immunosuppressive therapy. These findings may be helpful when planning for the initial treatment and may provide evidence for informed consent about potential comorbidities throughout the treatment course.
• Enhanced Systemic Antitumour Immunity by Hypofractionated Radiotherapy and Anti-PD-L1 Therapy in Dogs with Pulmonary Metastatic Oral Malignant Melanoma.
  Tatsuya Deguchi; Naoya Maekawa; Satoru Konnai; Ryo Owaki; Kenji Hosoya; Keitaro Morishita; Motoji Nakamura; Tomohiro Okagawa; Hiroto Takeuchi; Sangho Kim; Ryohei Kinoshita; Yurika Tachibana; Madoka Yokokawa; Satoshi Takagi; Yukinari Kato; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi
  Cancers, 15, 11, 01 Jun. 2023, [International Magazine]
  English, Scientific journal, Although immune checkpoint inhibitors (ICIs), such as the anti-programmed death-ligand 1 (PD-L1) antibody, have been developed for the treatment of canine malignant melanoma, desirable clinical efficacies have not been achieved. Recent studies in humans have suggested that radiation therapy (RT) combined with ICIs induces robust systemic antitumour immunity in patients with cancer. This study retrospectively examined the therapeutic efficacy of combination therapy (hypofractionated RT and anti-PD-L1 antibody [c4G12]) in dogs with pulmonary metastatic oral malignant melanoma. The intrathoracic clinical benefit rate (CBR)/median overall survival (OS) in the no RT (n = 20, free from the effect of RT), previous RT (n = 9, received RT ≤8 weeks prior to the first c4G12 dose), and concurrent RT (n = 10, c4G12 therapy within ±1 week of the first RT fraction) groups were 10%/185 days, 55.6%/283.5 days (p < 0.05 vs. no RT group), and 20%/129 days (p > 0.05 vs. no RT group), respectively. The adverse events were considered to be tolerable in the combination therapy. Thus, hypofractionated RT before the initiation of c4G12 therapy can be an effective approach for enhancing the therapeutic efficacy of immunotherapy, with acceptable safety profiles. Further prospective clinical studies are required to confirm the findings of this study.
• Evaluation of responses to immunosuppressive therapy in dogs with suspected non‐regenerative immune‐mediated anaemia: 11 cases (2012‐2018)
  K. Morishita; M. Sugawara‐Suda; J. Yamazaki; N. Sasaki; K. Nakamura; H. Ohta; M. Takiguchi
  Journal of Small Animal Practice, Wiley, 07 Apr. 2023, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• Transcriptome and proteome analysis of dogs with precursor targeted immune-mediated anemia treated with splenectomy.
  Mei Sugawara-Suda; Keitaro Morishita; Osamu Ichii; Takashi Namba; Keisuke Aoshima; Yumiko Kagawa; Sangho Kim; Kenji Hosoya; Nozomu Yokoyama; Noboru Sasaki; Kensuke Nakamura; Jumpei Yamazaki; Mitsuyoshi Takiguchi
  PloS one, 18, 5, e0285415, 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Precursor-targeted immune-mediated anemia (PIMA) in dogs is characterized by persistent non-regenerative anemia and ineffective erythropoiesis, and it is suspected to be an immune-mediated disease. Most affected dogs respond to immunosuppressive therapies; however, some are resistant. In this study, we carried out splenectomy as an alternative therapy for refractory PIMA in dogs, and analyzed gene expression levels in the spleen of dogs with or without PIMA and in serum before and after splenectomy. A total of 1,385 genes were found to express differentially in the spleens from dogs with PIMA compared with healthy dogs by transcriptome analysis, of which 707 genes were up-regulated, including S100A12, S100A8, and S100A9 that are linked directly to the innate immune system and have been characterized as endogenous damage-associated molecular patterns. Furthermore, immunohistochemistry confirmed that S100A8/A9 protein expression levels were significantly higher in dogs with PIMA compared with those in healthy dogs. A total of 22 proteins were found to express differentially between the serum samples collected before and after splenectomy by proteome analysis, of which 12 proteins were up-regulated in the samples before. The lectin pathway of complement activation was identified by pathway analysis in pre-splenectomy samples. We speculated that S100A8/9 expression may be increased in the spleen of dogs with PIMA, resulting in activation of the lectin pathway before splenectomy. These findings further our understanding of the pathology and mechanisms of splenectomy for PIMA.
• Serum steroid profiling of hepatocellular carcinoma associated with hyperadrenocorticism in dogs: A preliminary study
  Thandar Oo; Noboru Sasaki; Yoshinori Ikenaka; Takahiro Ichise; Noriyuki Nagata; Nozomu Yokoyama; Kazuyoshi Sasaoka; Keitaro Morishita; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Frontiers in Veterinary Science, 9, Frontiers Media SA, 28 Sep. 2022
  Scientific journal, Background
  
  Hepatocellular carcinoma (HCC) is one of the most common primary liver tumors in humans and dogs. Excessive adrenocortical hormone exposure may cause steroid hepatopathy, which may develop into HCC. In our previous study, hyperadrenocorticism (HAC) was a highly concurrent disease in dogs with HCC. Therefore, this study hypothesized that adrenal steroid alterations might be involved in hepatocarcinogenesis and aimed to specify the relationship between HAC and HCC in dogs.
  
  Materials and methods
  
  This study included 46 dogs brought to the Hokkaido University Veterinary Teaching Hospital between March 2019 and December 2020. Owners gave their signed consent for blood collection on their first visit. A total of 19 steroids (14 steroids and 5 metabolites) in the baseline serum of 15 dogs with HCC, 15 dogs with HAC, and 10 dogs with both diseases were quantitatively measured using the developed liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method.
  
  Results
  
  In each group, 11 steroids were detected higher than 50%. The detection rate of steroid hormones did not significantly differ between the groups (p &gt; 0.05). Principle component analysis (PCA) showed that the steroid profiles of the three groups were comparable. Median steroid hormone concentrations were not significantly different between the study diseases (p &gt; 0.05).
  
  Conclusion
  
  The developed LC/MS/MS was useful for measuring steroid hormones. Although it was clear that HAC was concurrent in dogs with HCC, none of the serum steroids was suggested to be involved in HCC.
• 磁気共鳴スペクトロスコピーは犬の起源不明髄膜脳脊髄炎の予後指標となり得るか
  山口 敬央; 笹岡 一慶; 新坊 弦也; 山根 由久; 永田 矩之; 横山 望; 森下 啓太郎; 佐々木 東; 中村 健介; 滝口 満喜
  北海道獣医師会雑誌, 66, 8, 287, 287, (公社)北海道獣医師会, Aug. 2022
  Japanese
• Urinary corticoid to creatinine ratios using IMMULITE 2000 XPi for diagnosis of canine hypercortisolism.
  Noriyuki Nagata; Hiryu Sawamura; Keitaro Morishita; Kenji Hosoya; Nozomu Yokoyama; Kazuyoshi Sasaoka; Noboru Sasaki; Kensuke Nakamura; Yoshinori Ikenaka; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 84, 7, 954, 959, 27 May 2022, [Domestic magazines]
  English, Scientific journal, The urinary corticoid to creatinine ratio (UCCR) is one of the most commonly used screening tests for canine hypercortisolism (HC). In this study, a reference interval was established for UCCR using IMMULITE 2000 XPi, the latest chemiluminescence enzyme immunoassay. The diagnostic performance of this method for UCCR in canine HC was also evaluated. The median UCCR was 1.06 × 10-5 (range: 0.28-2.49) for 58 healthy dogs, and an upper reference limit of 1.98 × 10-5 (90% confidence interval: 1.76-2.15) was determined. The median UCCR in the 12 dogs with HC (7.38 × 10-5, range 1.86-29.98) was significantly higher than that in the 16 dogs with mimic-HC (1.59 × 10-5, range 0.47-3.42, P<0.001). The area under the curve for UCCR to differentiate HC dogs from mimic-HC dogs was 0.971, with a sensitivity of 91.7% and specificity of 100% when the cut-off value was set at 3.77 × 10-5. The UCCR of 16 paired urine samples collected at home and in hospital showed that the UCCR of samples collected in the hospital was significantly higher than that of samples collected at home (mean difference 3.30 × 10-5, 95% confidence interval: 0.70-5.90, P=0.001). In summary, we established the upper reference limit for UCCR using IMMULITE 2000 XPi in dogs and confirmed that UCCR is a useful diagnostic test for HC in dogs if urine samples are collected at home.
• Local cerebral blood flow assessment using transcranial Doppler ultrasonography in a dog with brain infarction in the right middle cerebral artery territory
  Kazuyoshi SASAOKA; Hiroshi OHTA; Tomohito ISHIZUKA; Tatsuyuki OSUGA; Keitaro MORISHITA; Noboru SASAKI; Mitsuyoshi TAKIGUCHI
  Journal of Veterinary Medical Science, 84, 10, 1385, 1390, Japanese Society of Veterinary Science, 2022
  Scientific journal
• Prognostic value of left ventricular-arterial coupling estimated using echocardiography in dogs with myxomatous mitral valve disease.
  Tatsuyuki Osuga; Tomoya Morita; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 35, 6, 2607, 2615, 06 Nov. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: The interaction between the left ventricle (LV) and systemic arterial systems, known as left ventricular-arterial coupling (VAC), has been evaluated based on the effective arterial elastance (Ea) to LV end-systolic elastance (Ees) ratio (Ea/Ees). The Ea reflects the total arterial load of LV, whereas Ees reflects the LV systolic function. A recent study found that inappropriate VAC based on increased Ea/Ees estimated by echocardiography is associated with advanced disease severity in dogs with myxomatous mitral valve disease (MMVD). HYPOTHESIS: Inappropriate VAC assessed by echocardiographic estimation of Ea/Ees is associated with a worse prognosis in dogs with MMVD. ANIMALS: Eighty-nine dogs with MMVD. METHODS: Prospective cohort study. Dogs underwent echocardiographic examinations at enrollment. The Ea was estimated using the formula: mean blood pressure/(forward stroke volume/body weight). The Ees was estimated using the formula: mean blood pressure/(LV end-systolic volume/body weight). The Ea/Ees was calculated. RESULTS: By end of study, 22 dogs died of cardiac-related causes with 67 dogs censored. Dogs with increased Ea/Ees (Ea/Ees >0.34; median survival time, 527 days; 95% confidence interval [CI], 322 days-not determinable) had a shorter survival time (P < .0001) than those without increased Ea/Ees (Ea/Ees ≤0.34; median survival time, >1112 days; 95% CI, not determinable). Multivariate Cox proportional hazard analysis showed that Ea/Ees, body weight, peak systolic mitral annular velocity, and the peak early diastolic transmitral velocity-to-peak early diastolic mitral annular velocity ratio were independent predictors of cardiac-related death among echocardiographic indices. CONCLUSIONS AND CLINICAL IMPORTANCE: Inappropriate VAC assessed based on echocardiographically-estimated Ea/Ees is associated with a worse prognosis in dogs with MMVD.
• Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method.
  Noboru Sasaki; Thandar Oo; Yoshikazu Yasuda; Takahiro Ichise; Noriyuki Nagata; Nozomu Yokoyama; Kazuyoshi Sasaoka; Keitaro Morishita; Shouta Mm Nakayama; Mayumi Ishizuka; Kensuke Nakamura; Mitsuyoshi Takiguchi; Yoshinori Ikenaka
  The Journal of veterinary medical science, 83, 11, 1634, 1642, 17 Sep. 2021, [Domestic magazines]
  English, Scientific journal, We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2-16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.
• Extrahepatic biliary obstruction can interfere with hepatic fibrosis prediction using two-dimensional shear wave elastography in dogs.
  Masahiro Tamura; Hiroshi Ohta; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Mitsuyoshi Takiguchi
  Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association, 62, 4, 483, 489, Jul. 2021, [International Magazine]
  English, Scientific journal, Two-dimensional shear wave elastography (2D-SWE) can be used to quantitatively evaluate the elastic modulus of the liver as shear wave velocity (SWV), which can noninvasively predict clinically relevant hepatic fibrosis in both dogs and humans. However, extrahepatic biliary obstruction (EHBO), regardless of the presence of clinically relevant hepatic fibrosis, can influence SWVs in humans and thus may interfere with hepatic fibrosis prediction using 2D-SWE in dogs. The aim of this prospective, observational, and one-group pretest-posttest study is to investigate whether SWV measured by 2D-SWE displays a difference between dogs with and without EHBO. A total of 20 dogs were included (7 with EHBO and 13 with gallbladder pathology but no EHBO) that underwent preoperative SWV measurement using 2D-SWE. In all dogs, stages of hepatic fibrosis were evaluated histopathologically using a scoring scheme. In addition, postoperative SWVs in dogs with EHBO relieved via laparotomy were also evaluated. The median (range) SWVs in the dogs with and without EHBO were 1.91 (1.81-2.54) m/s and 1.57 (1.37-1.64) m/s, respectively. Although there was no significant difference in the histopathological hepatic fibrosis stages between the dogs with and without EHBO, the preoperative SWVs in the dogs with EHBO were significantly higher than in dogs without EHBO (P = .0004), and SWVs were found to decrease significantly after surgery (P = .0097). This study demonstrates that EHBO can increase the SWV of dogs without clinically relevant hepatic fibrosis and can interfere with the prediction of noninvasive hepatic fibrosis using 2D-SWE.
• Prognostic value of small intestinal dilatation in dogs with protein-losing enteropathy.
  Hiroshi Ohta; Noriyuki Nagata; Nozomu Yokoyama; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 83, 3, 378, 384, 11 Mar. 2021, [Domestic magazines]
  English, Scientific journal, To date, little is known about the prognostic significance of ultrasonographic findings in dogs with protein-losing enteropathy (PLE). The aim of this retrospective study was to examine the prognostic value of ultrasonographic findings in dogs with PLE. A total of 26 dogs with PLE were included: 20 dogs with chronic enteropathy and 6 dogs with gastrointestinal lymphoma. The presence of small intestinal dilatation was associated with shorter survival time in dogs with PLE (P=0.003). The presence of hyperechoic intestinal mucosal striations was associated with longer survival time in dogs with PLE (P=0.0085). The results of the current study indicate that the presence of small intestinal dilatation might be associated with poor prognosis in dogs with PLE.
• Surgical treatment of an abnormally positioned right adrenal tumor on segmental caudal vena cava aplasia in two dogs.
  Satoshi Takagi; Eiichi Kanai; Keitaro Morishita; Hiroko Ogawa; Junya Ogawa
  The Journal of veterinary medical science, 83, 1, 69, 72, 14 Jan. 2021, [Domestic magazines]
  English, Scientific journal, Segmental caudal vena cava (CVC) aplasia is a rare congenital vascular anomaly in dogs. Two dogs were diagnosed by CT imaging to have right adrenal tumors with concomitant segmental CVC aplasia. During surgery, a firm connection between the right adrenal gland and CVC was observed in both cases. The adrenal glands were found ventral to the CVC and the adrenal tumor was resected including the vascular wall. CVC venectomy for tumor removal will be required if the right adrenal gland is displaced in dogs with segmental CVC aplasia, even if there is no intravascular invasion.
• Echocardiographic estimation of left ventricular-arterial coupling in dogs with myxomatous mitral valve disease.
  Tatsuyuki Osuga; Tomoya Morita; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 35, 1, 78, 87, Jan. 2021, [International Magazine]
  English, Scientific journal, BACKGROUND: The effective arterial elastance (Ea) to left ventricular (LV) end-systolic elastance (Ees) ratio (Ea/Ees) is an index of the interaction between LV and systemic arterial systems, left ventricular-arterial coupling (VAC). The Ea is an index of total arterial load of the LV, whereas Ees is an index of LV systolic function. In humans, inappropriate VAC based on increased Ea/Ees estimated using echocardiography is associated with more advanced heart disease severity. HYPOTHESIS: Left ventricular-arterial coupling assessed by echocardiographic estimation of Ea/Ees is associated with disease severity in dogs with myxomatous mitral valve disease (MMVD). ANIMALS: Ninety MMVD dogs and 61 healthy dogs. METHODS: Prospective cross-sectional study. The MMVD dogs were classified into stages B1, B2, or C according to American College of Veterinary Internal Medicine guidelines. Effective arterial elastance was echocardiographically estimated using the formula: mean blood pressure/(forward stroke volume/body weight). End-systolic elastance was echocardiographically estimated using the formula: mean blood pressure/(LV end-systolic volume/body weight). The ratio Ea/Ees was calculated. RESULTS: The ratio Ea/Ees was higher in stage B2 dogs than in healthy dogs and dogs stage B1 (both P < .0001), and higher in stage C dogs than in healthy dogs and dogs in the other 2 stages (healthy vs C and B1 vs C, P < .0001; B2 vs C, P = .0005). Multivariable logistic regression analysis showed that Ea/Ees and the peak velocity of early diastolic transmitral flow to isovolumic relaxation time ratio were independent predictors of stage C among echocardiographic indices in MMVD dogs. CONCLUSIONS AND CLINICAL IMPORTANCE: Inappropriate VAC assessed by echocardiographically estimated Ea/Ees is associated with advanced disease severity in dogs with MMVD.
• Gene expression of leucine-rich alpha-2 glycoprotein in the polypoid lesion of inflammatory colorectal polyps in miniature dachshunds.
  Hiroshi Ohta; Yu Tamura; Nozomu Yokoyama; Noriyuki Nagata; Tatsuyuki Osuga; Noboru Sasaki; Yumiko Kagawa; Keitaro Morishita; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 82, 10, 1445, 1449, 20 Oct. 2020, [Domestic magazines]
  English, Scientific journal, Inflammatory colorectal polyps (ICRPs) in miniature dachshunds (MDs) are speculated to be a breed-specific inflammatory bowel disease (IBD). Leucine-rich alpha-2 glycoprotein (LRG) has been identified as a novel biomarker of human IBD. The aim of this study was to examine LRG gene expression in the polypoid lesions of ICRPs. Polypoid lesion specimens were collected from 24 MDs with ICRPs. Nonpolypoid colonic mucosa was collected from 18 MDs with ICRPs and 10 controls. The gene expression of LRG, interleukin (IL)-1β, IL-6, tumor necrosis factor-α, and IL-22 was examined. The expression of LRG gene was significantly increased in the polypoid lesions of ICRPs and correlated with that of the four cytokines. In conclusion, the LRG gene was expressed within the polypoid lesions of ICRPs and might be associated with local cytokine expression.
• An area ratio of thyroid gland to common carotid artery for evaluating the thyroid gland size.
  Noboru Sasaki; Noriyuki Nagata; Keitaro Morishita; Tatsuyuki Osuga; Kazuyoshi Sasaoka; Nozomu Yokoyama; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 82, 7, 1012, 1016, 31 Jul. 2020, [Domestic magazines]
  English, Scientific journal, The feasibility of ultrasonographic measurement of thyroid gland area to common carotid artery (TG:CCA) was investigated. Twenty-one healthy, 12 hypothyroid and 18 non-thyroid illness (NTI) dogs were evaluated. The area of thyroid lobe and common carotid artery in right and left sides were measured using the same ultrasonographic images in transverse plane. The average of the right and left ratio was calculated as TG:CCA. The median TG:CCA of 21 healthy dogs was 1.53, and it did not correlate either body weight or age. The median TG:CCA of 12 hypothyroid dogs was 0.81, which was significantly lower than that of 18 NTI dogs (1.81, P<0.001). If the cut off value <1.12 was used, TG:CCA indicated hypothyroidism with a sensitivity of 100%, specificity of 83%, and accuracy of 90%. Our data indicated that TG:CCA was independent of both body weight, which may contribute to consistent measurement of thyroid size. The results of this study suggest that TG:CCA is a promising tool for diagnosing canine hypothyroidism.
• Activities of matrix metalloproteinase-2, matrix metalloproteinase-9, and serine proteases in samples of the colorectal mucosa of Miniature Dachshunds with inflammatory colorectal polyps.
  Noriyuki Nagata; Hiroshi Ohta; Arisa Yamada; Yong Bin Teoh; Osamu Ichii; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 81, 7, 572, 580, Jul. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To investigate the activities of gelatinases (matrix metalloproteinase [MMP]-2 and MMP-9) and serine proteases in the colorectal mucosa of Miniature Dachshunds (MDs) with inflammatory colorectal polyps (ICRPs). ANIMALS: 15 MDs with ICRPs and 5 dogs with non-ICRP-related large bowel diarrhea (controls). PROCEDURES: Zymographic methods were used to evaluate the activities of MMP-2, MMP-9, latent forms of MMP-2 and MMP-9 (pro-MMP-2 and pro-MMP-9), and serine proteases in inflamed and noninflamed tissue samples from MDs with ICRPs and in noninflamed tissue samples from control dogs. The associations of serine protease activities with MMP-2 or MMP-9 activity were also analyzed. RESULTS: Activities of pro-MMP-2 and pro-MMP-9 were detected in most tissue samples, regardless of the tissue type, whereas activities of MMP-2 and MMP-9 were not detected in control tissue samples. In the inflamed tissue samples from MDs with ICRPs, the activities of MMP-2, pro-MMP-9, and MMP-9 were significantly higher than those in the noninflamed tissue samples from those dogs. Serine protease activities were significantly higher in the inflamed and noninflamed tissue samples from MDs with ICRP, compared with findings for control tissue samples. A weak correlation was detected between serine protease activities and MMP-9 activity. CONCLUSIONS AND CLINICAL RELEVANCE: Study results suggested that gelatinase and serine protease activities are upregulated in the colorectal mucosa of MDs with ICRPs, possibly contributing to the pathogenesis of this disease through the functions of these enzymes in degradation of extracellular matrix and promotion of inflammatory cell migration and inflammatory responses.
• Clinical characteristics of dogs with food-responsive protein-losing enteropathy.
  Noriyuki Nagata; Hiroshi Ohta; Nozomu Yokoyama; Yong Bin Teoh; Khoirun Nisa; Noboru Sasaki; Tatsuyuki Osuga; Keitaro Morishita; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 34, 2, 659, 668, Mar. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: In dogs with protein-losing enteropathy (PLE), data on the clinical characteristics of food-responsive PLE (FR-PLE) remain scarce. OBJECTIVE: To determine the clinical characteristics of FR-PLE in dogs responsive to ultralow-fat diet (ULFD) management. ANIMALS: Thirty-three dogs diagnosed with PLE based on standard diagnostic criteria. METHODS: Retrospective review of medical records. Clinical findings were compared between dogs with FR-PLE (FR-PLE group) and those with immunosuppressant-responsive PLE (IR-PLE) or nonresponsive PLE (NR-PLE) (IR/NR-PLE group). The area under the curve (AUC) of a receiver operating characteristic curve was used to evaluate the ability of factors to differentiate the FR-PLE and IR/NR-PLE groups. Survival time was compared between the FR-PLE and IR/NR-PLE groups. RESULTS: Twenty-three dogs responded to ULFD management and were diagnosed with FR-PLE. The canine chronic enteropathy clinical activity index (CCECAI) was significantly lower in the FR-PLE group than in the IR/NR-PLE group (P < .001). The AUC of CCECAI for differentiating the FR-PLE group was 0.935 (95% confidence interval [CI], 0.845-1.000) with an optimal cutoff value of 8 (sensitivity, 0.826; specificity, 0.889). Survival times were significantly longer in the FR-PLE group (median, not reached) than in the IR/NR-PLE group (median, 432 days; P < .001). CONCLUSIONS AND CLINICAL IMPORTANCE: Dogs that respond to ULFD management and are diagnosed with FR-PLE are expected to have a favorable prognosis. Clinical scores, specifically the CCECAI, could be useful for differentiating FR-PLE from IR-PLE or NR-PLE.
• Computed tomographic features for differentiating benign from malignant liver lesions in dogs.
  Rommaneeya Leela-Arporn; Hiroshi Ohta; Genya Shimbo; Kiwamu Hanazono; Tatsuyuki Osuga; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 12, 1697, 1704, 18 Dec. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Thus far, there are few computed tomography (CT) characteristics that can distinguish benign and malignant etiologies. The criteria are complex, subjective, and difficult to use in clinical applications due to the high level of experience needed. This study aimed to identify practical CT variables and their clinical relevance for broadly classifying histopathological diagnoses as benign or malignant. In this prospective study, all dogs with liver nodules or masses that underwent CT examination and subsequent histopathological diagnosis were included. Signalments, CT findings and histopathological diagnoses were recorded. Seventy liver nodules or masses in 57 dogs were diagnosed, comprising 18 benign and 52 malignant lesions. Twenty-three qualitative and quantitative CT variables were evaluated using univariate and stepwise multivariate analyses, respectively. Two variables, namely, the postcontrast enhancement pattern of the lesion in the delayed phase (heterogeneous; odds ratio (OR): 14.7, 95% confidence interval (CI): 0.82-262.03, P=0.0429) and the maximal transverse diameter of the lesion (>4.5 cm; OR: 33.3, 95% CI: 2.29-484.18, P=0.0006), were significantly related to the differentiation of benign from malignant liver lesions, with an area under the curve of 0.8910, representing an accuracy of 88.6%. These findings indicate that features from triple-phase CT can provide information for distinguishing pathological varieties of focal liver lesions and for clinical decision making. Evaluations of the maximal transverse diameter and postcontrast enhancement pattern of the lesion included simple CT features for predicting liver malignancy with high accuracy in clinical settings.
• Change in right ventricular function in an American cocker spaniel with acute pulmonary thromboembolism.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Kiwamu Hanazono; Keitaro Morishita; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 9, 1259, 1265, 03 Sep. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, A 12-year-old neutered female American cocker spaniel weighing 9.9 kg was presented for evaluation with a 2-day history of dyspnea and anorexia. Echocardiography revealed severe pulmonary hypertension (estimated systolic pulmonary arterial pressure, 93.4 mmHg) with right heart enlargement, pulmonary arterial dilation, and right ventricular dysfunction. The dilation of left heart and congenital cardiac shunt were not observed. Pulmonary thromboembolism (PTE) was confirmed by computed tomographic angiography. After treatment with antiplatelet and anticoagulant, the clinical sign and the echocardiographic abnormality of right heart were improved. These echocardiographic findings are not specific for PTE, but it can be useful as a rule-in test for PTE when other causes of pulmonary hypertension are excluded and a monitor of therapeutic efficacy.
• Usefulness of noninvasive shear wave elastography for the assessment of hepatic fibrosis in dogs with hepatic disease.
  Masahiro Tamura; Hiroshi Ohta; Genya Shimbo; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Yumiko Kagawa; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 33, 5, 2067, 2074, Sep. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Two-dimensional shear wave elastography (2D-SWE) can noninvasively evaluate hepatic elastic modulus as shear wave velocity (SWV). Additionally, it may predict the presence of clinical relevant hepatic fibrosis (≥F2) in dogs with hepatic disease. OBJECTIVES: To investigate whether SWV measured by 2D-SWE can differentiate between dogs with (≥F2) and without (F0-1) clinically relevant hepatic fibrosis. ANIMALS: Twenty-eight client-owned dogs with hepatic disease and 8 normal healthy Beagle dogs were enrolled. METHODS: In this cross-sectional prospective study, SWVs were measured using 2D-SWE in all dogs. Hepatic fibrosis stages and necroinflammatory activity grades were histopathologically evaluated using a histological scoring scheme that was adapted from the Ishak schema used in human medicine. RESULTS: Median SWVs were significantly higher in dogs with clinically relevant hepatic fibrosis (2.04 m/s; range, 1.81-2.26 m/s) than in healthy dogs (1.51 m/s; range, 1.44-1.66 m/s; P = .007), and dogs without clinically relevant hepatic fibrosis (1.56 m/s; range, 1.37-1.67 m/s; P < .001). However, no significant difference was found in the SWVs between dogs without clinically relevant hepatic fibrosis and healthy dogs (P = .99). Furthermore, median SWVs were not significantly different among dogs with necroinflammatory activity, those without necroinflammatory activity, and healthy dogs (Kruskal-Wallis test, P = .12). CONCLUSIONS AND CLINICAL IMPORTANCE: The 2D-SWE may be useful for predicting the presence of hepatic fibrosis in dogs with hepatic disease.
• Findings of contrast-enhanced ultrasonography with Sonazoid for cholangiocellular adenoma in three dogs.
  Masahiro Tamura; Kensuke Nakamura; Tatsuyuki Osuga; Genya Shimbo; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 8, 1104, 1108, 09 Aug. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Contrast-enhanced ultrasonography (CEUS) is useful to distinguish benign and malignant focal liver lesions in dogs. Cholangiocellular adenoma is an extremely rare benign tumor in dogs and has not been examined using CEUS with Sonazoid. The aim of this study was to describe findings of CEUS with Sonazoid in three dogs with cholangiocellular adenoma. All three dogs showed contrast defects in the Kupffer phase and these findings mimicked malignant neoplasia during the Kupffer phase. Moreover, all dogs showed early washout and hypoechoic lesions relative to the surrounding normal liver parenchyma in the portal phase. To our knowledge, this is the first study to report that CEUS findings of cholangiocellular adenoma with Sonazoid mimicked malignancy in three dogs.
• Effect of ultra low fat diet on regulatory T cells of dogs with intestinal limphangiectasia secondary to chronic enteropathy: A pilot study
  Khoirun Nisa; Hiroshi Ohta; Nozomu Yokoyama; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Rommaneeya Leela-Arporn; Tomoya Morita; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Yumiko Kagawa; Mitsuyoshi Takiguchi
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 67, 3, 221, 227, Aug. 2019, [Peer-reviewed]
  English, Scientific journal
• Plasma-free amino acid profiles in dogs with hepatocellular carcinoma.
  Rommaneeya Leela-Arporn; Hiroshi Ohta; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Khoirun Nisa; Tatsuyuki Osuga; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 33, 4, 1653, 1659, Jul. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Metabolomic analysis using blood samples has been suggested to be useful for the early detection of cancer. Among metabolites, plasma-free amino acid (PFAA) profiles are potential diagnostic biomarkers for several diseases including cancer. However, the relationship between PFAA concentrations and liver tumors in dogs remains unknown. OBJECTIVE: To determine the characteristics of PFAA profiles of dogs with hepatocellular carcinoma (HCC) and correlated clinical features. ANIMALS: Thirty-four client-owned dogs diagnosed with HCC (n = 26) and benign liver diseases (n = 8) and 11 age-matched healthy dogs. METHODS: Prospective study using heparinized blood samples from fasted dogs. Plasma was deproteinized, and the concentrations of 21 amino acids were measured using an automated high-performance liquid chromatography amino acid analyzer. RESULTS: Plasma glutamic acid concentrations were significantly different among groups (P < .0024 after Bonferroni correction). Compared to healthy dogs, dogs with HCC and benign liver diseases had significantly higher concentrations of glutamic acid by post hoc analysis. However, no significant difference in the PFAA profiles of HCC and benign liver diseases were detected. In addition, preoperative and postoperative PFAA profiles of dogs with HCC were not significantly different. CONCLUSIONS AND CLINICAL IMPORTANCE: Increased glutamic acid concentrations might play a role in the development or be a consequence of liver tumor formation. However, PFAA profiles of HCC could not be differentiated from those of benign lesions. In addition, glutamic acid concentrations did not change after surgical resection. These results indicate that PFAA profiles may not be useful biomarkers for detecting HCC in dogs.
• Plasma amino acid profiles in dogs with inflammatory bowel disease.
  Yu Tamura; Hiroshi Ohta; Yumiko Kagawa; Tatsuyuki Osuga; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 33, 4, 1602, 1607, Jul. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Lymphocytic-plasmacytic enteritis is the common form of idiopathic inflammatory bowel disease (IBD) in dogs. In human IBD, disturbances of amino acid metabolism have been demonstrated to be involved in the pathophysiology of IBD. Therefore, plasma amino acid profile might represent a novel marker of human IBD. OBJECTIVES: To determine the plasma amino acid profiles of dogs with IBD and its usefulness as a novel marker of IBD in dogs. ANIMALS: Fasting blood plasma was obtained from 10 dogs with IBD and 12 healthy dogs. METHODS: All IBD dogs were prospectively included in this study, and heparinized blood samples were collected. The plasma concentrations of 21 amino acids were determined using the ninhydrin method. The relationships among the plasma amino acid concentrations and plasma C-reactive protein (CRP) concentration, canine chronic enteropathy clinical activity index (CCECAI), and overall World Small Animal Veterinary Association (WSAVA) score were investigated. RESULTS: Median concentration (nmol/mL) of methionine [46.2; range, 30.0-59.3], proline [119.4; range, 76.7-189.2], serine [115.1; range, 61.4-155.9], and tryptophan [17.4; range, 11.9-56.3]) were significantly lower than in control dogs [62.6; range, 51.0-83.6, 199.1; range, 132.5-376.7, 164.3; range, 124.7-222.9, and 68.3; range, 35.7-94.8, respectively]. A negative correlation was identified between the plasma serine concentration and CCECAI (r s = -.67, P = .03), but there were no correlations between plasma amino acid concentrations and CRP concentration or overall WSAVA score. CONCLUSIONS AND CLINICAL IMPORTANCE: Plasma serine concentration might represent a novel maker of IBD in dogs.
• Epidemiology of massive hepatocellular carcinoma in dogs: A 4-year retrospective study.
  Leela-Arporn R; Ohta H; Nagata N; Sasaoka K; Tamura M; Dermlim A; Nisa K; Morishita K; Sasaki N; Nakamura K; Takagi S; Hosoya K; Takiguchi M
  Veterinary journal (London, England : 1997), 248, 74, 78, Elsevier BV, Jun. 2019, [Peer-reviewed]
  Scientific journal
• Right ventricular function and dyssynchrony measured by echocardiography in dogs with precapillary pulmonary hypertension.
  Morita, T; Nakamura, K; Osuga, T; Morishita, K; Sasaki, N; Ohta; H. Takiguchi, M
  Journal of Veterinary Cardiology, 23, 1, 1, 14, Elsevier BV, Jun. 2019, [Peer-reviewed]
  English, Scientific journal
• Predictive factors of malignancy in dogs with focal liver lesions using clinical data and ultrasonographic features.
  Rommaneeya Leela-Arporn; Hiroshi Ohta; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Khoirun Nisa; Keitaro Morishita; Noboru Sasaki; Kensuke Nakamura; Satoshi Takagi; Kenji Hosoya; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 5, 723, 729, 31 May 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, A definitive diagnosis of focal liver lesions (FLLs) requires invasive procedures for histopathologic examination. Thus, a simpler noninvasive diagnostic method, such as conventional ultrasonography combined with clinical data, is needed for the prediction of liver malignancy. The objective of this study was to examine the diagnostic accuracy of clinical data and ultrasonographic (US) features to differentiate benign and malignant liver lesions. Medical records and US images from dogs with FLLs that underwent abdominal US and histopathologic examinations following surgery or liver biopsy were retrospectively reviewed. Clinical data, including signalment, clinical signs and laboratory findings, and the US features of liver lesions that could act as predictive factors were assessed using univariate and multivariate analyses to evaluate the associations between predictive factors and liver malignancy. Based on the histopathologic results, 55 dogs with malignant lesions and 28 dogs with benign lesions were included in the study. The results of univariate analysis showed that several US features and platelet count were significantly associated with liver malignancy. Multivariate analysis revealed that the platelet count (thrombocytosis; odds ratio [OR]: 4.13, 95% confidence intervals [CI]: 1.81-9.41), lesion size (4.1 cm or greater; OR: 23.83, 95% CI: 3.74-151.95) and echotexture of FLLs (heterogenous; OR: 8.44; 95% CI: 1.37-51.91) were independent predictors for differentiating benign and malignant liver lesions, suggesting that a combination of clinical data and US findings of FLLs could predict liver malignancy in dogs.
• Evaluation of liver and spleen stiffness of healthy dogs by use of two-dimensional shear wave elastography.
  Masahiro Tamura; Hiroshi Ohta; Khoirun Nisa; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Mitsuyoshi Takiguchi
  American journal of veterinary research, 80, 4, 378, 384, Apr. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To assess liver and spleen stiffness in healthy dogs by use of a novel 2-D shear wave elastography (SWE) technique and to investigate the repeatability and reproducibility of the technique. ANIMALS: 8 healthy adult Beagles. PROCEDURES: 2-D SWE was performed on each dog to assess liver and spleen stiffness. Repeatability (intraday variability) and reproducibility (interday variability) of 2-D SWE were investigated. For all 8 dogs, 2-D SWE was performed 3 times in 1 day (4-hour intervals) and on 3 separate days (1-week interval). Data were expressed as mean ± SD values for shear wave velocity and the Young modulus in the liver and spleen. Intraday and interday coefficients of variation were assessed for all variables. RESULTS: Mean ± SD shear wave velocity obtained for the liver and spleen was 1.51 ± 0.08 m/s and 2.18 ± 0.27 m/s, respectively. Mean value for the Young modulus obtained for the liver and spleen was 6.93 ± 0.79 kPa and 14.66 ± 3.79 kPa, respectively. Elasticity values were significantly higher for the spleen than for the liver. Intraday and interday coefficients of variation for all variables were < 25% (range, 3.90% to 20.70%). CONCLUSIONS AND CLINICAL RELEVANCE: 2-D SWE was a feasible technique for assessing liver and spleen stiffness of healthy dogs. Future studies on the application of 2-D SWE for dogs with chronic hepatitis, cirrhosis, and portal hypertension are needed to evaluate the clinical applicability of 2-D SWE.
• Evaluation of duodenal perfusion by contrast-enhanced ultrasonography in dogs with chronic inflammatory enteropathy and intestinal lymphoma.
  Nisa K; Lim SY; Shinohara M; Osuga T; Yokoyama N; Tamura M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 33, 2, 559, 568, Mar. 2019, [Peer-reviewed]
  English, Scientific journal
• Contrast-enhanced ultrasonography is a feasible technique for quantifying hepatic microvascular perfusion in dogs with extrahepatic congenital portosystemic shunts.
  Masahiro Tamura; Hiroshi Ohta; Khoirun Nisa; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Mitsuyoshi Takiguchi
  Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association, 60, 2, 192, 200, Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Extrahepatic-congenital portosystemic shunt is a vascular anomaly that connects the portal vein to the systemic circulation and leads to a change in hepatic microvascular perfusion. However, an assessment of hepatic microvascular perfusion is limited by conventional diagnostic modalities. The aim of this prospective, exploratory study was to assess hepatic microvascular perfusion in dogs with extrahepatic-congenital portosystemic shunt using contrast-enhanced ultrasonography (CEUS) using perfluorobutane (Sonazoid® ). A total of 17 dogs were included, eight healthy dogs and nine with extrahepatic-congenital portosystemic shunt. The time-to-peak (TTP), rising time (RT), and rising rate (RR) in the hepatic artery, portal vein, and hepatic parenchyma, as well as the portal vein-to-hepatic parenchyma transit time (ΔHP-PV) measured from time-intensity curve on CEUS were compared between healthy and extrahepatic-congenital portosystemic shunt dogs. The RT of the hepatic artery in extrahepatic-congenital portosystemic shunt dogs was significantly earlier than in healthy dogs (P = 0.0153). The TTP and RT of the hepatic parenchyma were significantly earlier in extrahepatic-congenital portosystemic shunt dogs than in healthy dogs (P = 0.0018 and P = 0.0024, respectively). ΔHP-PV was significantly shorter in extrahepatic-congenital portosystemic shunt dogs than in healthy dogs (P = 0.0018). CEUS effectively revealed changes in hepatic microvascular perfusion including hepatic artery, portal vein, and hepatic parenchyma simultaneously in extrahepatic-congenital portosystemic shunt dogs. Rapid hepatic artery and hepatic parenchyma enhancements may reflect a compensatory increase in hepatic artery blood flow (arterialization) caused by a decrease in portal vein blood flow and may be used as an additional diagnostic test to distinguish extrahepatic-congenital portosystemic shunt dogs from healthy dogs.
• Effect of acute volume overload on echocardiographic indices of right ventricular function and dyssynchrony assessed by use of speckle tracking echocardiography in healthy dogs.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Nozomu Yokoyama; Keitaro Morishita; Noboru Sasaki; Hiroshi Ohta; Mitsuyoshi Takiguchi
  American journal of veterinary research, 80, 1, 51, 60, Jan. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE To determine the relationship between acute volume overload and echocardiographic indices of right ventricular (RV) function and dyssynchrony in healthy dogs. ANIMALS 7 healthy Beagles. PROCEDURES Right heart catheterization and echocardiography were performed in 7 healthy anesthetized Beagles at baseline and after induction of volume overload. Volume overload was induced by IV infusion of lactated Ringer solution (150 mL/kg/h for 90 minutes). Echocardiographic indices of RV function, including peak velocity of systolic tricuspid annular motion, tricuspid annulus plane systolic excursion, fractional area change, RV Tei index, RV longitudinal strain (RVLS), and systolic RV longitudinal strain rate (RVLSR), were obtained by use of speckle tracking echocardiography (STE). In addition, SD of the systolic shortening time of the right ventricle for the 6 segments (RV-SD6) was determined with STE. RESULTS Volume overload significantly increased the RV end-diastolic pressure, compared with the baseline value. Echocardiographic indices of RV function, except for septal RVLSR, were significantly enhanced by volume overload. In contrast, RV-SD6 did not change with volume overload. Although echocardiographic indices of RV function, except for septal RVLSR, were correlated with RV end-diastolic pressure, RV-SD6 was not correlated. CONCLUSIONS AND CLINICAL RELEVANCE Echocardiographic indices of RV function, including RVLS and RVLSR, were affected by acute short-term volume overload. Therefore, results for assessment of RV function by use of STE in dogs with clinical conditions associated with right-sided chronic volume overload, such as tricuspid and pulmonic regurgitation, should be interpreted with caution.
• Corrigendum: Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats.
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in veterinary science, 6, 2, 2, 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, [This corrects the article DOI: 10.3389/fvets.2018.00289.].
• Effects of immunosuppressive prednisolone therapy on pancreatic tissue and concentration of canine pancreatic lipase immunoreactivity in healthy dogs.
  Hiroshi Ohta; Kazuki Kojima; Nozomu Yokoyama; Noboru Sasaki; Yumiko Kagawa; Kiwamu Hanazono; Tomohito Ishizuka; Keitaro Morishita; Kensuke Nakamura; Satoshi Takagi; Mitsuyoshi Takiguchi
  Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 82, 4, 278, 286, Oct. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The objective of this study was to examine the effects of immunosuppressive prednisolone therapy on pancreatic tissue and the concentration of serum canine pancreatic lipase immunoreactivity (cPLI) in healthy dogs. Six healthy beagle dogs were subcutaneously administered an immunosuppressive dose of prednisolone [4 mg/kg body weight (BW)] once daily for either 2 or 3 weeks. Serum cPLI concentration was measured before and after treatment. Ultrasonographic examination of the pancreas and laparoscopic biopsy and histopathological examination of the right pancreatic lobe and the liver were also conducted before and after treatment. The expression of pancreatic lipase messenger ribonucleic acid (mRNA) in the pancreas and liver was examined by polymerase chain reaction (PCR). Although the serum cPLI concentration was significantly higher on day 14 and on the day of the second laparoscopy than before treatment, it was classified as normal (≤ 200 μg/L) in 5 dogs and as abnormal (≥ 400 μg/L) in only 1 dog. None of the 6 dogs showed clinical signs of pancreatitis during the study period. After treatment, ultrasonographic examination of the pancreas showed no changes except for a hypoechoic pancreas in 1 dog. Histopathological examination of the right pancreatic lobe in all dogs showed no evidence of pancreatitis after treatment. Pancreatic lipase mRNA expression was detected in the pancreas, but not in the liver, before and after treatment. The administration of 4 mg/kg BW per day of prednisolone for 2 or 3 weeks increased the serum cPLI concentration without clinical signs of pancreatitis, although an abnormal cPLI concentration (≥ 400 μg/L) was observed in only 1 dog. No ultrasonographic or histological evidence of pancreatitis was observed in any of the dogs.
• The effect of sedation with a combination of butorphanol and midazolam on quantitative contrast-enhanced ultrasonography of duodenum in healthy dogs.
  Khoirun Nisa; Sue Yee Lim; Tatsuyuki Osuga; Nozomu Yokoyama; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Rommaneeya Leela-Arporn; Tomoya Morita; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 80, 3, 453, 459, 24 Mar. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Quantitative contrast-enhanced ultrasonography (CEUS) enables non-invasive and objective evaluation of intestinal perfusion by quantifying the intensity of enhancement on the intestine after microbubble contrast administration. During CEUS scanning, sedation is sometimes necessary to maintain animal cooperation. Nevertheless, the effect of sedative administration on the canine intestinal CEUS is unknown. This study aimed to investigate the effect of sedation with a combination of butorphanol and midazolam on the duodenal CEUS-derived perfusion parameters of healthy dogs. For this purpose, duodenum was imaged following contrast administration (Sonazoid®, 0.01 ml/kg) in six healthy beagles before and after intravenous injection of a combination of butorphanol (0.2 mg/kg) and midazolam (0.1 mg/kg). Furthermore, hemodynamic parameters including blood pressure and heart rate were recorded during the procedure. Five CEUS derived perfusion parameters including time-to-peak (TTP), peak intensity (PI), area under the curve (AUC), wash-in and wash-out rates (WiR and WoR, respectively) before and after sedation were statistically compared. The result showed that no significant change was detected in any of perfusion parameters. Systolic and mean arterial pressures significantly reduced after sedative administration, but diastolic arterial pressure and heart rate did not significantly change. Moreover, no significant partial correlation was observed between perfusion parameters and hemodynamic parameters. Thus, we concluded that the combination did not cause significant influence in duodenal CEUS perfusion parameters and could be a good option for sedation prior to duodenal CEUS in debilitated dogs.
• Transcranial Doppler Ultrasound Examination in Dogs with Suspected Intracranial Hypertension Caused by Neurologic Diseases
  Sasaoka K; Nakamura K; Osuga T; Morita T; Yokoyama N; Morishita K; Sasaki N; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 32, 1, 314, 323, 2018, [Peer-reviewed]
  Scientific journal
• Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats.
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in veterinary science, 5, 289, 289, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Increased incidence of kidney disease (KD) is a common concern in human and companion animals. Cats, in particular, are highly susceptible to KD. Novel KD biomarkers would help to address these problems. Therefore, we are focusing on microRNA, a highly conserved nucleic acid, as a KD biomarker for various animals. We previously reported that altered levels of urinary exosome (UExo)-derived microRNAs indicate renal pathologies in dogs. This study comprehensively examined UExo-derived microRNAs, which reflected the KD status in cats. The examined cats were divided into two groups: normal renal function (NR) and KD. Based on our previous data in dogs and cats, as well as the present data on UExo-derived microRNAs in cats by next-generation sequencing, let-7b, let-7f, miR-10a, miR-10b, miR-21a, miR-22, miR-26a, miR-27b, miR-146a, miR-181a, miR-191, and miR-486a were identified as biomarker candidates. In summary, the levels of UExo-derived let-7b, miR-22, and miR-26a significantly decreased in cats with KD from the early stages of the disease. UExo-derived miRNA levels normalized to urinary creatinine or total RNA of miR-21a was significantly higher in the KD group. Importantly, the ratio of UExo-derived miR-21a to let-7b showed a significant and strongest correlation with serum creatinine (ρ = 0.751), blood urea nitrogen (ρ = 0.754), and urinary creatinine (ρ = -0.421) among all examined indices. Further, the ratio of miR-181a to let-7b or miR-10b significantly correlated with the progression of renal dysfunction in the KD group. Thus, we identified that UExo-derived microRNAs in cats, and their raw and normalized levels could indicate altered renal function.
• Spatial and temporal profile of cisplatin delivery by ultrasound-assisted intravesical chemotherapy in a bladder cancer model
  Noboru Sasaki; Kazuhiro Ishi; Nobuki Kudo; Shouta M. M. Nakayama; Kensuke Nakamura; Keitaro Morishita; Hiroshi Ohta; Mayumi Ishizuka; Mitsuyoshi Takiguchi
  PLOS ONE, 12, 11, e0188093, Nov. 2017, [Peer-reviewed]
  English, Scientific journal
• Repeatability and reproducibility of quantitative contrast-enhanced ultrasonography for assessing duodenal perfusion in healthy dogs.
  Nisa K; Lim SY; Shinohara M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Yokoyama N; Osuga T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  The Journal of Veterinary Medical Science, 79, 9, 1585, 1590, Sep. 2017, [Peer-reviewed]
  English, Scientific journal
• Changes in right ventricular function assessed by echocardiography in dog models of mild RV pressure overload
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Nozomu Yokoyama; Keitaro Morishita; Noboru Sasaki; Hiroshi Ohta; Mitsuyoshi Takiguchi
  ECHOCARDIOGRAPHY-A JOURNAL OF CARDIOVASCULAR ULTRASOUND AND ALLIED TECHNIQUES, 34, 7, 1040, 1049, Jul. 2017, [Peer-reviewed]
  English, Scientific journal
• Stimulation of colorectal biopsies from miniature dachshunds with inflammatory colorectal polyps with toll-like receptor ligands: A pilot study
  Nozomu Yokoyama; Hiroshi Ohta; Yumiko Kagawa; Noriyuki Nagata; Khoirun Nisa; Tomoya Morita; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Mitsuyoshi Takiguchi
  VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 188, 78, 83, Jun. 2017, [Peer-reviewed]
  English, Scientific journal
• Washout Ratio in the Hepatic Vein Measured by Contrast-Enhanced Ultrasonography to Distinguish Between Inflammatory and Noninflammatory Hepatic Disorders in Dogs
  K. Morishita; A. Hiramoto; A. Michishita; S. Takagi; T. Osuga; S. Y. Lim; K. Nakamura; N. Sasaki; H. Ohta; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 31, 3, 770, 777, May 2017, [Peer-reviewed]
  English, Scientific journal
• An intrathoracic ectopic liver with pleural effusion in a dog
  Yoshimi Iwaki; Satoshi Takagi; Keitaro Morishita; Kiwamu Hanazono; Kenji Hosoya; Masahiro Okumura
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 65, 2, 95, 99, May 2017, [Peer-reviewed]
  English, Scientific journal
• Washout Ratio in the Hepatic Vein Measured by Contrast-Enhanced Ultrasonography to Distinguish Between Inflammatory and Noninflammatory Hepatic Disorders in Dogs
  K. Morishita; A. Hiramoto; A. Michishita; S. Takagi; T. Osuga; S. Y. Lim; K. Nakamura; N. Sasaki; H. Ohta; M. Takiguchi
  Journal of Veterinary Internal Medicine, 31, 3, 770, 777, Blackwell Publishing Inc., 01 May 2017, [Peer-reviewed]
  English, Scientific journal
• Assessment of contrast-enhanced ultrasonography of the hepatic vein for detection of hemodynamic changes associated with experimentally induced portal hypertension in dogs
  Keitaro Morishita; Akira Hiramoto; Asuka Michishita; Satoshi Takagi; Yuki Hoshino; Takaharu Itami; Sue Yee Lim; Tatsuyuki Osuga; Sayuri Nakamura; Kenji Ochiai; Kensuke Nakamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 78, 4, 465, 471, Apr. 2017, [Peer-reviewed]
  English, Scientific journal
• Expression of apical junction complex proteins in colorectal mucosa of miniature dachshunds with inflammatory colorectal polyps
  Nozomu Yokoyama; Hiroshi Ohta; Yumiko Kagawa; Rommaneeya Leela-Arporn; Angkhana Dermlim; Khoirun Nisa; Tomoya Morita; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Mitsuyoshi Takiguchii
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 79, 3, 456, 463, Mar. 2017, [Peer-reviewed]
  English, Scientific journal
• Serial measurement of pancreatic lipase immunoreactivity concentration in dogs with immune-mediated disease treated with prednisolone
  Ohta H; Morita T; Yokoyama N; Osuga T; Sasaki N; Morishita K; Nakamura K; Takiguchi M
  Journal of Small Animal Practice, 58, 6, 342, 347, 2017, [Peer-reviewed]
  Scientific journal
• Localization of Toll-like Receptor (TLR) 2 and TLR4 mRNA in the Colorectal Mucosa of Miniature Dachshunds with Inflammatory Colorectal Polyps
  Yokoyama N; Ohta H; Yamazaki J; Kagawa Y; Ichii O; Khoirun N; Morita T; Osuga T; Lim S.Y; Sasaki N; Morishita K; Nakamura K; Takiguchi M
  Journal of Comparative Pathology, 156, 2-3, 183, 190, 2017, [Peer-reviewed]
  Scientific journal
• Left Atrial Strain at Different Stages of Myxomatous Mitral Valve Disease in Dogs
  Nakamura K; Kawamoto S; Osuga T; Morita T; Sasaki N; Morishita K; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 31, 2, 316, 325, 2017, [Peer-reviewed]
• The repeatability and characteristics of right ventricular longitudinal strain imaging by speckle-tracking echocardiography in healthy dogs
  Morita T; Nakamura K; Osuga T; Yokoyama N; Khoirun N; Morishita K; Sasaki N; Ohta H; Takiguchi M
  Journal of Veterinary Cardiology, 19, 4, 351, 362, 2017, [Peer-reviewed]
  Scientific journal
• Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Yuki Hoshino; Satoshi Takagi; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yasuhiro Kon
  SCIENTIFIC REPORTS, 7, 40340, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• Contrast-enhanced ultrasonography of the hepatic vein in normal dogs
  Keitaro Morishita; Akira Hiramoto; Tatsuyuki Osuga; Sue Yee Lim; Nisa Khoirun; Noboru Sasaki; Kensuke Nakamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 78, 12, 1753, 1758, Dec. 2016, [Peer-reviewed]
  English, Scientific journal
• Effects of experimental cardiac volume loading on left atrial phasic function in healthy dogs
  Tatsuyuki Osuga; Kensuke Nakamura; Tomoya Morita; Khoirun Nisa; Nozomu Yokoyama; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 77, 9, 952, 960, Sep. 2016, [Peer-reviewed]
  English, Scientific journal
• Hypercalcemia in a Dog with Chronic Ingestion of Maxacalcitol Ointment
  Kensuke Nakamura; Nobuo Tohyama; Masahiro Yamasaki; Hiroshi Ohta; Keitaro Morishita; Mitsuyoshi Takiguchi
  JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION, 52, 4, 256, 258, Jul. 2016, [Peer-reviewed]
  English, Scientific journal
• Repeatability and reproducibility of right ventricular Tei index valves derived from three echocardiographic methods for evaluation of cardiac function in dogs
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Sue Yee Lim; Nozomu Yokoyama; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 77, 7, 715, 720, Jul. 2016, [Peer-reviewed]
  English, Scientific journal
• Prognostic Value of Right Ventricular Tei Index in Dogs with Myxomatous Mitral Valvular Heart Disease
  Nakamura K; Morita T; Osuga T; Morishita K; Sasaki N; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 30, 1, 69, 75, Wiley-Blackwell, 2016, [Peer-reviewed]
  English
• Effects of various cardiovascular drugs on indices obtained with two-dimensional speckle tracking echocardiography of the left atrium and time-left atrial area curve analysis in healthy dogs
  Tatsuyuki Osuga; Kensuke Nakamura; Tomoya Morita; Sue Yee Lim; Nozomu Yokoyama; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 76, 8, 702, 709, Aug. 2015, [Peer-reviewed]
  English, Scientific journal
• Vitamin D Status in Different Stages of Disease Severity in Dogs with Chronic Valvular Heart Disease
  Osuga T; Nakamura K; Morita T; Lim S.Y; Nisa K; Yokoyama N; Sasaki N; Morishita K; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 29, 6, 1518, 1523, 2015, [Peer-reviewed]
  Scientific journal
• Quantitative Contrast-enhanced Ultrasonographic Assessment of Naturally Occurring Pancreatitis in Dogs
  S. Y. Lim; K. Nakamura; K. Morishita; N. Sasaki; M. Murakami; T. Osuga; N. Yokoyama; H. Ohta; M. Yamasaki; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 29, 1, 71, 78, Jan. 2015, [Peer-reviewed]
  English, Scientific journal
• CONTRAST-ENHANCED ULTRASONOGRAPHIC FINDINGS IN THREE DOGS WITH PANCREATIC INSULINOMA
  Kensuke Nakamura; Sue-Yee Lim; Kenji Ochiai; Masahiro Yamasaki; Hiroshi Ohta; Keitaro Morishita; Satoshi Takagi; Mitsuyoshi Takiguchi
  VETERINARY RADIOLOGY & ULTRASOUND, 56, 1, 55, 62, Jan. 2015, [Peer-reviewed]
  English, Scientific journal
• Evaluation of Selected Cytokine Gene Expression in Colonic Mucosa from Dogs with Idiopathic Lymphocytic-plasmacytic Colitis
  Yu Tamura; Hiroshi Ohta; Nozomu Yokoyama; Sue Yee Lim; Tatsuyuki Osuga; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, 10, 1407, 1410, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• In vitro and in vivo safety and efficacy Studies of amphotericin B on Babesia gibsoni
  Masahiro Yamasaki; Eriko Harada; Yu Tamura; Sue Yee Lim; Tatsuyuki Ohsuga; Nozomu Yokoyama; Keitaro Morishita; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  VETERINARY PARASITOLOGY, 205, 3-4, 424, 433, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• Case report: Protein-losing enteropathy caused by Mesocestoides vogae (syn. M. corti) in a dog
  Yu Tamura; Hiroshi Ohta; Takuya Kashiide; Jun Matsumoto; Tatsuya Sakurai; Nozomu Yokoyama; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY PARASITOLOGY, 205, 1-2, 412, 415, Sep. 2014, [Peer-reviewed]
  English, Scientific journal
• Expression of apical junction complex proteins in duodenal mucosa of dogs with inflammatory bowel disease
  Hiroshi Ohta; Yuji Sunden; Nozomu Yokoyama; Tatsuyuki Osuga; Sue Yee Lim; Yu Tamura; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 75, 8, 746, 751, Aug. 2014, [Peer-reviewed]
  English, Scientific journal
• Qualitative and Quantitative Contrast-Enhanced Ultrasonographic Assessment of Cerulein-Induced Acute Pancreatitis in Dogs
  S. Y. Lim; K. Nakamura; K. Morishita; N. Sasaki; M. Murakami; T. Osuga; H. Ohta; M. Yamasaki; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 28, 2, 496, 503, Mar. 2014, [Peer-reviewed]
  English, Scientific journal
• Prognostic Value of Left Atrial Function in Dogs with Chronic Mitral Valvular Heart Disease
  Nakamura K; Osuga T; Morishita K; Suzuki S; Morita T; Yokoyama N; Ohta H; Yamasaki M; Takiguchi M
  Journal of Veterinary Internal Medicine, 28, 6, 1746, 1752, Wiley-Blackwell, 2014, [Peer-reviewed]
  English
• Qualitative and Quantitative Contrast Enhanced Ultrasonography of the Pancreas Using Bolus Injection and Continuous Infusion Methods in Normal Dogs
  Sue Yee Lim; Kensuke Nakamura; Keitaro Morishita; Noboru Sasaki; Masahiro Murakami; Tatsuyuki Osuga; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 75, 12, 1601, 1607, Dec. 2013, [Peer-reviewed]
  English, Scientific journal
• Markedly increased expression of interleukin-8 in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds
  Yu Tamura; Hiroshi Ohta; Shidow Torisu; Masashi Yuki; Nozomu Yokoyama; Masahiro Murakami; Sue Yee Lim; Tatsuyuki Osuga; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 156, 1-2, 32, 42, Nov. 2013, [Peer-reviewed]
  English, Scientific journal
• Repeatability and reproducibility of measurements obtained via two-dimensional speckle tracking echocardiography of the left atrium and time-left atrial area curve analysis in healthy dogs
  Tatsuyuki Osuga; Kensuke Nakamura; Sue Yee Lim; Yu Tamura; Wickramasekara Rajapakshage Bandula Kumara; Masahiro Murakami; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 74, 6, 864, 869, Jun. 2013, [Peer-reviewed]
  English, Scientific journal

• 猫の心筋症における左房ブースターストレインの有用性の検討
  佐藤景; 中村健介; 川元誠; 菅原芽伊; 笹岡一慶; 横山望; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 121st, 2024
• 犬の発作性ジスキネジア23例の回顧的検討
  川上侑記; 笹岡一慶; 大田寛; 山根由久; 新坊弦也; 横山望; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 67, 8, 2023
• 2次診療動物病院に来院した犬の糞便真核生物叢解析
  横山 望; 川口 剛; 中尾 亮; 森下 啓太郎; 細谷 謙次; 永田 矩之; 笹岡 一慶; 新坊 弦也; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 165回, [HS1P, 16], Sep. 2022
  (公社)日本獣医学会, Japanese
• 非再生性免疫介在性貧血の犬における脾臓摘出術前後の血清中蛋白の網羅的解析
  菅原 芽伊; 山崎 淳平; 森下 啓太郎; 金 尚昊; 細谷 謙次; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 60], Sep. 2021
  (公社)日本獣医学会, Japanese
• 【犬と猫における輸血療法の実際2021】輸血後紫斑病および急性溶血性輸血反応を疑った犬の1例
  森下 啓太郎, MVM, 30, 5, 53, 56, Jul. 2021
  (株)ファームプレス, Japanese
• 症例で学ぶ検査値の落とし穴 第13回 自己免疫検査 性ホルモン検査
  森下啓太郎; 小林正典, SA Medicine, 23, 4, 2021
• 診療力が試される症例 Vet Board QUIZ 血液 6)
  田川道人 森下啓太郎, Veterinary Board, 3, 7, 2021
• 診療力が試される症例 Vet Board QUIZ 血液 Question 1:血小板減少症の原因を考察し、最も可能性が高い病態をあげてください。 Question 2:症例の臨床経過と血液塗抹所見(図1)から、「腫瘍+血小板減少症+再生性貧血」の相互関係を考察してください
  森下 啓太郎, Veterinary Board, 2, 12, 58, 63, Dec. 2020
  (株)エデュワードプレス, Japanese
• 【最新輸液療法〜症例から正しい輸液の概念を見直す〜】(Vol.11)膵炎の輸液管理
  石塚 友人; 森下 啓太郎, SA Medicine, 22, 5, 5, 24, Oct. 2020
  (株)エデュワードプレス, Japanese
• 非再生性貧血のミニチュア・ダックスフンド11例に対する脾臓摘出術の治療成績
  菅原 芽伊; 森下 啓太郎; 今井 健友; 山崎 淳平; 佐々木 東; 大田 寛; 細谷 謙次; 滝口 満喜, SA Medicine, 22, 3, 74, 75, Jun. 2020
  (株)エデュワードプレス, Japanese
• 【最新輸液療法〜症例から正しい輸液の概念を見直す〜】(Vol.7)肝疾患の輸液管理
  森下 啓太郎, SA Medicine, 22, 1, 3, 20, Feb. 2020
  (株)エデュワードプレス, Japanese
• とことん掘り下げ!PSS 超音波検査でシャント血管を描出するコツ
  森下啓太郎, GO-VET, 4, 1, 2020
• What’s your diagnosis?第10回
  森下啓太郎, GO-VET, 3, 2, 2020
• 【輸血のABCと副反応】輸血概論
  森下 啓太郎, Veterinary Board, 2, 1, 6, 16, Jan. 2020
  (株)エデュワードプレス, Japanese
• 診療力が試される症例 Vet Board QUIZ 血液 Question 以下の症例情報から、貧血の鑑別診断を4つあげてください
  森下 啓太郎, Veterinary Board, 2, 1, 50, 55, Jan. 2020
  (株)エデュワードプレス, Japanese
• 抗がん薬治療の実際〜薬用量の決定と有害事象への対応(第12回) (CASE 27)犬の慢性骨髄性白血病
  永田 矩之; 森下 啓太郎, Veterinary Oncology, 7, 1, 103, 109, Jan. 2020
  (株)エデュワードプレス, Japanese
• ジェネラリストのための目で見て覚える 腹部X線・超音波の異常像(第15回) 胃
  森下 啓太郎, CAP: Companion Animal Practice, 34, 7, 56, 65, Jul. 2019
  (株)緑書房, Japanese
• ジェネラリストのための目で見て覚える腹部X線・超音波の異常像(第13回) 脾臓
  森下 啓太郎, CAP: Companion Animal Practice, 34, 5, 58, 64, May 2019
  (株)緑書房, Japanese
• What’s your diagnosis?第6回
  森下啓太郎, GO-VET, 2, 2, 61‐65, 20 Mar. 2019
  Japanese
• ピルビン酸キナーゼ欠損症 (特集 猫の疾患 総まとめ(後編)) -- (品種特異的疾患)
  森下 啓太郎, Clinic note : journal of clinical daily treatment for small animals, 15, 3, 36, 39, Mar. 2019
  インターズー, Japanese
• 蛋白喪失性腸症の犬40例からの腸リンパ管拡張症の診断と治療に関する考察
  永田矩之; 大田寛; 横山望; 佐々木東; 大菅辰幸; 森下啓太郎; 滝口満喜, 日本獣医師会獣医学術学会年次大会講演要旨集, 2018, 2019
• LC-MS/MSを用いたイヌ尿中メタネフリンおよびノルメタネフリン測定
  井上裕美子; 佐々木東; 池中良徳; 一瀬貴大; 笹岡一慶; 大菅辰幸; 中山翔太; 森下啓太郎; 大田寛; 石塚真由美; 滝口満喜, 日本獣医学会学術集会講演要旨集, 162nd, 2019
• こうすればよかった 見逃し・後悔症例カンファレンス Vol.3 Case 11 おしっこに血が混ざってるんですけど...
  森下啓太郎, SA Medicine, 21, 3, 2019
• What’s your diagnosis?第7回
  森下啓太郎, GO-VET, 2, 3, 2019
• What’s your diagnosis?第8回
  森下啓太郎, GO-VET, 2, 4, 2019
• What’s your diagnosis?第9回
  森下啓太郎, GO-VET, 3, 1, 2019
• 猫の疾患|総まとめ(前編)腎泌尿器疾患‐慢性腎臓病と尿石症‐
  森下啓太郎, Clinic Note, 15, 1, 43‐53, 01 Jan. 2019
  Japanese
• 腎泌尿器疾患 : 慢性腎臓病と尿石症 (特集 猫の疾患 総まとめ(前編))
  森下 啓太郎, Clinic note : journal of clinical daily treatment for small animals, 15, 1, 43, 53, Jan. 2019
  インターズー, Japanese
• What’s your diagnosis?第5回
  森下啓太郎, GO-VET, 2, 1, 87‐91, 20 Dec. 2018
  Japanese
• 腎性貧血 (特集 臨床徴候シリーズ(vol. 5)貧血)
  森下 啓太郎, J-vet : jounral for veterinary practitioner, 31, 10, 50, 55, Oct. 2018
  インターズー, Japanese
• What’s your diagnosis?第4回
  森下啓太郎, GO-VET, 1, 4, 103‐108, 20 Sep. 2018
  Japanese
• 神経筋接合部検査としてアイスパックテストを試みた重症筋無力症の犬の1例
  山田 有紗; 笹岡 一慶; 大田 寛; 立花 由莉加; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 335, 335, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 犬の粘液腫様変性性僧帽弁疾患における心エコー図法による左心室-大動脈カップリングの推定
  大菅 辰幸; 佐々木 東; 森下 啓太郎; 大田 寛; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 335, 335, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 神経症状を主訴とした悪性中皮腫の猫の1例
  大村 侑己; 大田 寛; 小林 篤史; 笹岡 一慶; 道下 明日香; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 340, 340, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 経頭蓋超音波ドプラ法により局所的な脳循環動態を評価し得た右中大脳動脈梗塞の犬の1例
  笹岡 一慶; 大田 寛; 石塚 友人; 大菅 辰幸; 立花 由莉加; 森下 啓太郎; 佐々木 東; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 341, 341, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 鼻腔内真菌感染症に対しクロトリマゾール灌流および注入療法を実施した犬の1例
  大谷 豪; 大田 寛; 岩木 有香; 細谷 謙次; 新坊 弦也; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 344, 344, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 貧血 (特集 腫瘍随伴症候群 : 知る! 診る! 治す! そして活かす!)
  森下 啓太郎, Veterinary oncology : 小動物腫瘍科専門誌, 5, 3, 41, 46, Jul. 2018
  インターズー, Japanese
• What’s your diagnosis?第3回
  森下啓太郎, GO-VET, 1, 3, 79‐85, 20 Jun. 2018
  Japanese
• What’s your diagnosis?第2回
  森下啓太郎, GO-VET, 1, 2, 83‐88, 20 Mar. 2018
  Japanese
• 血液疾患 非再生性免疫介在性貧血 (特集 ミニチュア・ダックスフンドに好発する免疫介在性疾患)
  森下 啓太郎, Clinic note : journal of clinical daily treatment for small animals, 14, 3, 34, 39, Mar. 2018
  インターズー, Japanese
• 蛋白喪失性腸症の犬33例からの腸リンパ管拡張症の診断と治療に関する考察
  永田矩之; 大田寛; 横山望; ニサ ホイルン; 佐々木東; 森下啓太郎; 滝口満喜, 北海道獣医師会雑誌, 62, 8, 2018
• シスプラチン膀胱内ソノポレーションの前臨床試験的検討
  佐々木東; 中山翔太; 大田寛; 大菅辰幸; 森下啓太郎; 水川葉月; 池中良徳; 石塚真由美; 滝口満喜, 日本獣医学会学術集会講演要旨集, 161st, 2018
• JCVIM 2018 臨床研究アワード 慢性僧帽弁疾患犬において右室strainによる右心室機能評価は優れた予後指標となり得るか (第14回日本獣医内科学アカデミー学術大会(JCVIM 2018) 〈臨床研究アワード〉日本獣医内科学アカデミー/インターズー学術奨励賞受賞作品ダイジェスト版)
  森田 智也; 中村 健介; 大菅 辰幸; 森下 啓太郎; 佐々木 東; 大田 寛; 滝口 満喜, SA medicine : Journal of small animal medicine, 24, 3, 68, 70, 2018
  インターズー, Japanese
• 不明熱に対する診断アプローチ (特集 不明熱の診断プロセス)
  森下 啓太郎, Clinic note : journal of clinical daily treatment for small animals, 13, 12, 14, 21, Dec. 2017
  インターズー, Japanese
• What’s your diagnosis?第1回
  森下啓太郎, GO-VET, 1, 1, 118‐123, 20 Nov. 2017
  Japanese
• 【一目でわかる症候シリーズ Vol.15 黄疸】 肝臓の原発性腫瘍
  森下 啓太郎, SA Medicine, 19, 3, 28, 31, Jun. 2017
  (株)インターズー, Japanese
• 【一目でわかる症候シリーズ Vol.15 黄疸】 肝臓の転移性腫瘍
  森下 啓太郎, SA Medicine, 19, 3, 32, 35, Jun. 2017
  (株)インターズー, Japanese
• 全エクソームシーケンスを用いたミニチュア・ダックスフントの炎症性結直腸ポリープ感受性遺伝子の探索
  永田矩之; 大田寛; 横山望; 田村昌大; 笹岡一慶; LEELA-ARPORN Rommaneeya; DERMLIM Angkhana; NISA Khoirun; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160th, 2017
• 副腎偶発腫から診断した犬のサブクリニカルクッシング症候群の臨床的特徴
  永田矩之; 大田寛; 佐々木東; 森下啓太郎; 中村康介; 中村康介; 細谷謙次; 高木哲; 滝口満喜, 北海道獣医師会雑誌, 61, 8, 2017
• 犬虚血性脳血管障害における脳底動脈血流の経頭蓋超音波ドプラ法による評価
  笹岡一慶; 大田寛; 田村昌大; 永田矩之; LEELA-ARPORN Rommaneeya; DERMLIM Angkhana; KHOIRUN Nisa; 森田智也; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160th, 2017
• 犬慢性腸症の十二指腸粘膜におけるロイシンリッチα2糖蛋白の発現とその臨床的意義
  奥朋哉; 大田寛; 田村昌大; 永田矩之; 笹岡一慶; LEELA-ARPORN Rommaneeya; DERMLIM Angkhana; NISA Khoirun; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160th, 2017
• 心エコー図検査にて急性期および回復後の変化を捉えた急性肺血栓塞栓症の犬の1症例
  森田智也; 中村健介; 森下啓太郎; 竹内恭介; 大菅辰幸; 那須香菜子; 華園究; 滝口満喜, 北海道獣医師会雑誌, 60, 8, 417, 417, 10 Aug. 2016
  (公社)北海道獣医師会, Japanese
• ソマトトロピン産生性下垂体腫瘍によりインスリン抵抗性を呈した糖尿病の猫の1例
  和泉 雄介; 細谷 謙治; 早川 小百合; 森下 啓太郎; 高木 哲; 奥村 正裕, 日本獣医師会雑誌, 69, 5, 293, 293, May 2016
  (公社)日本獣医師会, Japanese
• 大腸血管拡張症の犬の1症例
  竹内恭介; 大田寛; 高木哲; 中島彩乃; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 60, 8, 2016
• 犬の膵炎診断における客観的で簡便な指標による超音波検査の有用性の検討
  竹内恭介; 中村健介; 森下啓太郎; 大田寛; 佐々木東; 滝口満喜, 日本獣医師会獣医学術学会年次大会講演要旨集, 2015 (CD-ROM), 2016
• 【症候シリーズvol.6 貧血(2)・出血傾向】 血球貪食性組織球肉腫
  森下 啓太郎, SA Medicine, 17, 6, 20, 23, Dec. 2015
  (株)インターズー, Japanese
• 犬のびまん性肝疾患に対する造影超音波検査の応用の試み
  道下 明日香; 森下 啓太郎; 華園 究; 中村 健介; 滝口 満喜; 高木 哲, 日本獣医画像診断学会抄録, 58回, 12, 12, Sep. 2015
  (一社)日本獣医画像診断学会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるToll-like receptor2,4の局在解析
  横山望; 大田寛; 賀川由美子; 山崎淳平; 市居修; KHOIRUN Nisa; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 日本獣医学会学術集会講演要旨集, 158th, 2015
• 犬におけるSpeckle tracking法を用いた右室strain解析の再現性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.2, 216, 216, Dec. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• うっ血性左心不全を呈した原発性心臓血管肉腫の犬の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; TOOYAMA NOBUO; MORITA TOMOYA; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 33, 34, Nov. 2014
  動物臨床医学会, Japanese
• 免疫抑制量のプレドニゾロンが奏功した重度肺高血圧症を伴う間質性肺疾患を疑ったウェルシュコーギーの1例
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 103, 104, Nov. 2014
  動物臨床医学会, Japanese
• NSAIDsが原因と考えられた骨髄壊死の犬の1例
  MORISHITA KEITARO; DEGUCHI TATSUYA; KAGAWA YUMIKO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 93, 94, Nov. 2014
  動物臨床医学会, Japanese
• 免疫介在性貧血の骨髄像
  森下 啓太郎, MPアグロジャーナル, 19, 7, 10, Oct. 2014
  MPアグロ, Japanese
• プロカルバジンが奏功した肉芽腫性髄膜脳脊髄炎を疑う犬の1例
  YAMAGUCHI TOMOO; OTA HIROSHI; MATSUMOTO MASANORI; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 365, 365, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 肺高血圧症の犬に対するDual pulsed‐wave Doppler法を用いた右室Tei‐index測定の有用性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 369, 369, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 尿管結石による水腎症から膿腎症に至った犬の1例
  HAYAKAWA SAYURI; NAKAMURA KENSUKE; TAKAGI SATOSHI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 376, 376, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 二次的な巨大食道を伴った鼻咽頭ポリープの猫の1例
  NASU KANAKO; NAKAMURA KENSUKE; TAKAGI SATOSHI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 374, 374, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 活性型ビタミンD₃外用薬の慢性摂取により高カルシウム血症が認められた犬の2例
  SHIMOIDE ARISA; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 379, 379, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 肝静脈造影超音波検査による肝疾患罹患犬の肝内血行動態の評価
  MORISHITA KEITARO; HIRAMOTO AKIRA; RIN SU・I; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 370, 370, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 左心房機能は心血管薬によりいかに変化するか:正常犬における2D Speckle Tracking法を用いた検討
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORITA TOMOYA; YEE LIM SUE; YOKOYAMA NOZOMU; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 157回, 479, 479, Aug. 2014
  (公社)日本獣医学会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるclaudinの蛋白質発現解析
  YOKOYAMA NOZOMU; OTA HIROSHI; MORITA TOMOYA; OSUGA TATSUYUKI; LIM SUE YEE; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 157回, 491, 491, Aug. 2014
  (公社)日本獣医学会, Japanese
• 犬胃癌10症例における超音波検査所見
  YOKOYAMA NOZOMI; OTA HIROSHI; NAKAMURA KENSUKE; MORISHITA KEITARO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 261, 261, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 徐脈性不整脈に対してピモベンダンを投与し失神の改善を認めた犬の1例
  MORITA TOMOYA; SUZUKI SHUJI; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 248, 248, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 造影超音波検査による腎機能評価の基礎的検討
  MORISHITA KEITARO; RIN SU・I; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 262, 262, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 2D Speckle Tracking法を用いた左心房機能評価による犬心筋症の重症度評価
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; SUZUKI SHUJI; MORISHITA KEITARO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 67, 5, 367, 367, 20 May 2014
  (公社)日本獣医師会, Japanese
• プレドニゾロンを主とする免疫抑制療法実施症例における犬膵特異的リパーゼの推移
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 67, 5, 369, 369, May 2014
  (公社)日本獣医師会, Japanese
• セルレイン誘発膵炎犬における造影超音波検査の定量的および定性的評価
  LIM SUE YEE; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医畜産新報, 67, 1113, 286, 288, 01 Apr. 2014
  文永堂出版, Japanese
• Dual pulsed‐wave doppler法による右室Tei‐index測定の再現性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; SUZUKI SHUJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 44, Suppl.2, 250, 250, Dec. 2013
  (一社)日本獣医麻酔外科学会, Japanese
• 猫心筋症において2D Speckle Tracking法を用いた左心房機能評価は可能であるのか
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; SUZUKI SHUJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 44, Suppl.2, 249, 249, Dec. 2013
  (一社)日本獣医麻酔外科学会, Japanese
• 両側性尿管閉塞を呈した犬の1例
  TOOYAMA NOBUO; NAKAMURA KENSUKE; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; TAKAGI SATOSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 3, 4, Nov. 2013
  動物臨床医学会, Japanese
• 非典型的溶血性尿毒症症候群(D‐HUS)が疑われた犬の1例
  MORISHITA KEITARO; TOOYAMA NOBUO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 141, 142, Nov. 2013
  動物臨床医学会, Japanese
• 慢性特発性偽性腸閉塞症が疑われた犬の1例
  YOKOYAMA NOZOMU; OTA HIROSHI; HASHIBA KANO; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OCHIAI KENJI; TERAMOTO HIDESHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 59, 60, Nov. 2013
  動物臨床医学会, Japanese
• 市販フードの給餌によって病的骨折を起こした栄養性二次性上皮小体機能亢進症の犬の1例
  HASHIBA KANO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 119, 120, Nov. 2013
  動物臨床医学会, Japanese
• 非典型的溶血性尿毒症症候群(D‐HUS)が疑われた犬の1例
  TOOYAMA NOBUO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 395, 395, Aug. 2013
  (公社)北海道獣医師会, Japanese
• プレドニゾロンを主とする免疫抑制療法実施症例における犬膵特異的リパーゼの推移
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 402, 402, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 輪状咽頭アカラシアの犬の1例
  UENO TAKASHI; OTA HIROSHI; TAKAGI SATOSHI; HOSHI KIYOTAKA; MORISHITA KEITARO; NAKAMURA TAKESHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 401, 401, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 肝静脈血流波形解析による右心不全の重症度評価
  NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 66, 5, 346, 347, May 2013
  (公社)日本獣医師会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープの病態
  OTA HIROSHI; TAMURA YU; YOKOYAMA NOZOMI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 155回, 153, 153, Mar. 2013
  (公社)日本獣医学会, Japanese
• 末梢肺動脈狭窄症と診断した高齢猫の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.2, 213, 213, Dec. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• 好酸球増多症候群を伴った消化器型リンパ腫の猫の1例
  TOOYAMA NOBUO; HOSOYA KENJI; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 399, 399, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 麻酔後の全脳虚血が疑われた2症例のMRI所見
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 429, 429, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 肝静脈血流波形解析による右心不全の重症度評価
  NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 409, 409, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 下垂体性副腎皮質機能亢進症と中枢性尿崩症を併発し,放射線治療を行った下垂体腫瘍の犬の1例
  TAMURA YU; YAMASAKI MASAHIRO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 410, 410, Aug. 2012
  (公社)北海道獣医師会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ病変における炎症性サイトカインの発現解析
  TAMURA YU; OTA HIROSHI; MURAKAMI MASAHIRO; BANDULA KUMARA WR; SUE YEE LIM; OSUGA TATSUYUKI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 154回, 307, 307, Aug. 2012
  (公社)日本獣医学会, Japanese
• 房室ブロックの犬2例に認められた低速僧帽弁逆流と高速左室駆出血流
  NAKAMURA KENSUKE; TOOYAMA NOBUO; UENO TAKASHI; MORISHITA KEITARO; OSUGA TATSUYUKI; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 245, 245, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の頭蓋内疾患診断における経頭蓋超音波ドプラ法の有用性の検討
  SASAOKA KAZUYOSHI; NAKAMURA KENSUKE; MORISHITA KEITARO; OSUGA TATSUYUKI; LIM SUE YEE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 270, 270, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• CT検査を実施した肝葉捻転の犬の2例
  MORISHITA KEITARO; HOSOYA KENJI; TAKAGI SATOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 267, 267, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• Two Feline Cases Which Developed Severe Hemolytic Anemia after Treatment for Diabetic Ketoacidosis
  MASATSUGU Hideaki; YASUKAWA Kuniyoshi; OHIGASHI Yusuke; MORISHITA Keitaro; NAKAMICHI Jun; FUKUI Kenta; SHIMODA Tetsuya, 動物臨床医学 = Journal of Animal Clinical Research Foundation, 19, 1, 15, 19, 20 Mar. 2010
  Japanese
• 犬の膵疾患における超音波ヒストグラム解析の有用性に関する基礎的研究
  NAKAMURA KENSUKE; MORISHITA KEITARO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 144th, 160, 160, 27 Aug. 2007
  (公社)日本獣医学会, Japanese
• クエン酸シルデナフィルが奏功した逆行性動脈管開存症の犬の2例
  NAKAMURA KENSUKE; MORISHITA KEITARO; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 51, 8, 333, 333, 01 Aug. 2007
  (公社)北海道獣医師会, Japanese
• メソトレキセートが奏功したステロイド抵抗性炎症性腸疾患の犬の1例
  MORISHITA KEITARO; NAKAMURA KENSUKE; HIRAYAMA KAZUKO; TANIYAMA HIROYUKI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 51, 8, 335, 335, 01 Aug. 2007
  (公社)北海道獣医師会, Japanese
• デジタルX線(DR)撮影下における非選択的血管造影法により逆行性動脈管開存症と診断された犬の2例
  MORISHITA KEITARO; NAKAMURA KENSUKE; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 日本獣医循環器学会抄録, 86th, Suppl.1, 203, 203, 2007
  (一社)日本獣医麻酔外科学会, Japanese
• I型偽性低アルドステロン症が疑われた犬の1例
  MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 336, 80, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• 犬の慢性リンパ性白血病:メルファラン継続投与による良好な長期コントロールの1例
  HIROSE TAKESHI; NAKAMURA KENSUKE; MORISHITA KEITARO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 346, 90, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• デジタルX線(DR)撮影下における非選択的血管造影法により逆行性動脈管開存症と診断された犬の1例
  TOOYAMA NOBUO; NAKAMURA KENSUKE; MORISHITA KEITARO; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 355, 99, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• 十二指腸に閉塞性病変を伴った炎症性陽疾患の犬の1例
  中村 健介; 森下 啓太郎; 廉澤 剛; 平山 和子; 谷山 弘行; 滝口 満喜, 北海道獣医師会雑誌, 50, 8, 79, 79, Aug. 2006
  (公社)北海道獣医師会, Japanese

• 犬の内科診療
  森下啓太郎, 鉄欠乏性貧血、腎性貧血、慢性疾患に伴う貧血
  緑書房, Mar. 2021, 9784895315852, 503p, Japanese, [Contributor]
• 猫の治療ガイド : 私はこうしている
  森下啓太郎, 門脈圧亢進症、
  Eduward press, Aug. 2020, 9784866711225, xvi, 963, 23p, Japanese, [Contributor]
• 犬の治療ガイド : 私はこうしている
  森下啓太郎, 門脈圧亢進症、免疫介在性好中球減少症、非再生性免疫介在性貧血、血球貪食性組織球性肉腫
  Eduward press, Aug. 2020, 9784866711218, xvi, 1219, 28p, Japanese, [Contributor]
• 猫の診療指針
  森下啓太郎, 鉄欠乏性貧血、腎性貧血、慢性疾患に伴う貧血
  緑書房, Aug. 2017, 9784895313100, 冊, Japanese, [Contributor]

• 初期診断が困難であった血球貪食性組織球性肉腫の犬の１例
  森下啓太郎
  日本獣医臨床病理学会2021年次大会, Oral presentation
  18 Aug. 2021 - 07 Sep. 2021
• 骨髄の評価
  森下啓太郎
  第17回日本獣医内科学アカデミー学術大会, Feb. 2021, Invited oral presentation
  [Invited]
• 免疫介在性溶血性貧血の診断と治療 米国獣医内科学会consensus statement準拠
  森下 啓太郎
  動物臨床医学会年次大会プロシーディング, Nov. 2020, 動物臨床医学会, Japanese
  Nov. 2020 - Nov. 2020, [Invited]
• ＡＣＶＩＭコンセンサスガイドラインからおさえるＩＭＨＡ治療編
  森下啓太郎
  第16回日本獣医内科学アカデミー学術大会, Feb. 2020, Invited oral presentation
  [Invited]
• 顕微鏡ＤＥディスカッション
  森下啓太郎
  第39回動物臨床医学会年次大会, Nov. 2019, Nominated symposium
  [Invited]
• 免疫介在性溶血性貧血（IMHA）の診断
  森下啓太郎
  第15回日本獣医内科学アカデミー学術大会, Feb. 2019, Invited oral presentation
  [Invited]
• 非再生性貧血の診断と治療
  森下啓太郎
  第15回日本獣医内科学アカデミー学術大会, Feb. 2019, Invited oral presentation
  [Invited]
• 尿中メタネフリン分画測定が診断の決め手となった副腎偶発腫の犬の2例
  立花由莉加; 森下啓太郎; 高木哲; 大田寛; 佐々木東; 大菅辰幸; 滝口満喜
  第14回日本獣医内科学アカデミー学術大会, 17 Feb. 2018
  17 Feb. 2018
• 血液疾患と間違えやすい病態を理解する
  森下啓太郎
  第14回日本獣医内科学アカデミー学術大会, Feb. 2018, Invited oral presentation
  [Invited]
• up-date ヘプシジンの発見で変わった 腎性貧血のメカニズム
  森下啓太郎
  第14回日本獣医内科学アカデミー学術大会, Feb. 2018, Invited oral presentation
  [Invited]
• 犬の非再生性免疫介在性貧血の臨床病理所見と治療反応に関する回顧的研究
  岩永優斗; 森下啓太郎; 細谷謙次; 大菅辰幸; 佐々木東; 大田寛; 滝口満喜
  北海道獣医師会雑誌, 2018
  2018 - 2018
• 感染性動脈瘤の猫の１例
  森下啓太郎; 高木哲; 賀川由美子; 華園究; 佐々木東; 大田寛; 滝口満喜
  第9回北海道・九州小動物獣医療ジョイントシンポジウム, 17 Dec. 2017
• 猫のCKDの治療最前線 腎性貧血の病態と治療
  森下 啓太郎
  動物臨床医学会年次大会プロシーディング, Nov. 2017, 動物臨床医学会, Japanese, Nominated symposium
  Nov. 2017 - Nov. 2017, [Invited]
• 生前診断に苦慮した化膿性腸炎の猫の1例
  森下啓太郎; 中島彩乃; 賀川由美子; 華園究; 佐々木東; 大田寛; 滝口満喜
  日本獣医画像診断学会抄録(Web), Sep. 2017, (一社)日本獣医画像診断学会, Japanese
  Sep. 2017 - Sep. 2017
• 犬の原発性免疫介在性血小板減少症(pIMT)再発症例の回顧的研究
  高村大地; 森下啓太郎; 中島彩乃; 竹内恭介; 佐々木東; 大田寛; 滝口満喜
  北海道獣医師会雑誌, Aug. 2017, (公社)北海道獣医師会, Japanese
  Aug. 2017 - Aug. 2017
• 市販フードの給餌により栄養性二次性上皮小体機能亢進症を生じた犬の2例
  道下明日香; 森下啓太郎; 橋場香野; 田村昌大; 金尚昊; 佐々木東; 大田寛; 滝口満喜
  北海道獣医師会雑誌, Aug. 2017, (公社)北海道獣医師会, Japanese
  Aug. 2017 - Aug. 2017
• 後天性門脈体循環シャントを合併した先天性肝線維症の猫の1例
  田村昌大; 森下啓太郎; 高木哲; 早川小百合; 賀川由美子; 佐々木東; 大田寛; 滝口満喜
  北海道獣医師会雑誌, Aug. 2017, (公社)北海道獣医師会, Japanese
  Aug. 2017 - Aug. 2017
• Remarked megakaryocytic dysplasia in a dog with non-regenerative anemia
  森下啓太郎
  日本獣医臨床病理学会2017年大会, 24 Jun. 2017, Oral presentation
• 犬の原発性免疫介在性血小板減少症（pIMT）の回顧的研究
  高村大地, 森下啓太郎, 中島彩乃, 竹内恭介, 大菅辰幸, 佐々木東, 大田寛, 滝口満喜
  第14回日本獣医内科学アカデミー学術大会, 17 Feb. 2017
• 造影超音波検査による門脈高血圧の新しい診断法
  森下啓太郎
  第13回日本獣医内科学アカデミー学術大会, Feb. 2017, Invited oral presentation
  [Invited]
• 非再生性免疫介在性貧血(NRIMA)を徹底攻略 NRIMAの治療 脾摘は有効な手段か？
  森下啓太郎
  第13回日本獣医内科学アカデミー学術大会, Feb. 2017, Invited oral presentation
  [Invited]
• 好塩基球性白血病の犬の1症例
  渡邉清正; 森下啓太郎; 中村健介; 佐々木東; 大田寛; 滝口満喜
  北海道獣医師会雑誌, Aug. 2016, (公社)北海道獣医師会, Japanese
  Aug. 2016 - Aug. 2016
• 肝膵疾患の超音波検査～基礎から応用まで～造影超音波検査の応用
  第12回日本獣医内科学アカデミー学術大会, Feb. 2016, Invited oral presentation
  [Invited]
• クイズで学ぶ腹部画像診断
  森下啓太郎
  第11回日本獣医内科学アカデミー学術大会, 22 Feb. 2015, Public discourse
  [Invited]
• 臨床現場で必要な血液学入門
  森下啓太郎
  北海道小動物獣医師会年次大会2014, 02 Nov. 2014, Public discourse
  [Invited]
• 肝静脈血流指標による門脈圧亢進症の非侵襲的評価の試み
  森下啓太郎
  第56回日本獣医画像診断学会, 09 Sep. 2014, Public discourse
  [Invited]
• 貧血の鑑別診断
  森下啓太郎
  北海道九州小動物獣医療ジョイントシンポジウム2013, 07 Dec. 2013, Public discourse
  [Invited]

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• アドバンスト演習, 2024年, 学士課程, 獣医学部
• 総合獣医療実習, 2024年, 学士課程, 獣医学部
• 獣医コミュニケーション演習, 2024年, 学士課程, 獣医学部
• 伴侶動物獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 伴侶動物獣医療実習Ⅱ, 2024年, 学士課程, 獣医学部
• 夜間・救急獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 総合獣医療実習, 2024年, 学士課程, 獣医学部
• アドバンスト演習, 2024年, 学士課程, 獣医学部
• アドバンスト演習, 2024年, 学士課程, 獣医学部
• アドバンスト演習, 2024年, 学士課程, 獣医学部
• 内科学各論, 2024年, 学士課程, 獣医学部
• プレクリニカル実習, 2024年, 学士課程, 獣医学部

• JAPANESE SOCIETY OF CLINICAL VETERINARY MEDICINE
• THE JAPANESE SOCIETY OF VETERINARY IMAGINGS
• JAPAN VETERINARY MEDICAL ASSOCIATION
• JAPANESE SOCIETY OF VETERINARY SCIENCE

• 脾臓摘出がいかにして犬の非再生貧血を改善させるのか
  科学研究費助成事業
  01 Apr. 2024 - 31 Mar. 2027
  森下 啓太郎
  日本学術振興会, 若手研究, 北海道大学, 24K18009
• Mechanisms of TSPO2-mediated cholesterol redistribution in erythroblasts required for their effective proliferation and maturation
  Grants-in-Aid for Scientific Research
  01 Apr. 2023 - 31 Mar. 2026
  稲葉 睦; 森下 啓太郎
  TSPO2の細胞内分布・コレステロール分布との関係を主にマウスES細胞由来赤芽球系培養細胞(MEDEP)の正常(WT)細胞、Tspo2－/－細胞、ならびにTspo2－/－細胞にTSPO2-HAを導入した細胞(Tspo2－/－HA)を用いて調べた。
  1）Tspo2－/－細胞は遊離コレステロールをを小胞体ERでエステル型（CEs）に変換し脂肪滴(LD)に貯蔵する割合が低く、多くは細胞外に失われること、TSPO2-HAの発現でこの異常は解消すること、高コレステロールマウス血清（主にHDL由来のCEsが高濃度）はWTと同程度のCEs蓄積を生じることを明らかにした。
  2）MEDEP細胞にTSPO2-HAを導入・発現させると、Golgin、LAMP2、NPC1等との共在からゴルジ装置、リソソームに分布することが示された。同時にcalnexinやRab5, 7, 11等core Rabsとの近接から、各種エンドソーム、小胞体の近傍に位置することも示された。さらにclathrinやcatalaseとも近接した分布が観察された。Tspo2遺伝子をTspo2-HAに変換したMEDEP細胞でも同様の結果が得られた。TSPO2が主にゴルジやリソソームに分布し、小胞体をゴールとして、各種輸送小胞やペルオキシソーム、小胞体との間のコレステロール輸送に関与することが推定された。
  3）MEDEP細胞や骨髄由来赤芽球では、TSPO2と細胞内コレステロール両者の分布の相互関連を明確に示すことは困難であった（解像度の不足、ならびに細胞質が狭く明確な分布をしめすことの困難による）。そこでTSPO2-EGFPを導入したHEK293細胞で同様の解析を実施し、それが細胞内遊離コレステロールの分布とほぼ一致することが判明したが、どの小器官が関わるかまでは見同定である。一方でCEsとは異なる分布が明らかであった。
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23K27065
• ミニチュア・ダックスフンドに好発する非再生性貧血の病態解明
  科学研究費助成事業 若手研究
  01 Apr. 2019 - 31 Mar. 2022
  森下 啓太郎
  本邦のミニチュア・ダックスフンドには、原因不明の非再生性貧血が好発する。本研究では脾臓摘出によって造血能が劇的に回復するという知見を基に、貧血の原因をTGF-βシグナル異常に起因する造血障害としてとらえ、脾臓におけるTGF-βの過剰産生の有無、および骨髄におけるTGF-β制御因子の異常発現の有無を明らかにすることを目的とした。
  非再生貧血を呈するミニチュア・ダックス11頭で脾臓摘出術を実施した。年齢の中央値は12歳（範囲：5-15歳）、体重の中央値は5.12 kg（範囲：3.4-7.66 kg）であり、性別は雄4頭（全て去勢済）、雌7頭（避妊済み3頭）であった。術前の免疫抑制療法の実施日数は中央値112日（範囲：52-237日）、輸血間隔の中央値は34日（範囲：4-207 日）であった。脾摘による治療反応を評価したところ、7頭が有効、2頭が無効、2頭が治療判定不可に分類された。摘出された脾臓の病理組織診断は全例で髄外造血であり、罹患犬の脾臓には対照群（ミニチュアダックス以外の髄外造血サンプル）に比べ淡明核を有する巨核球の有意な増加を認めた。治療効果を判定可能であった9頭に対し脾臓におけるTGF-β関連分子のmRNA発現量をリアルタイムPCR法で定量したところ、脾摘有効群は無効群に比べTGF-β発現が高い傾向を得た。TGF-β受容体, SMAD-2, SMAD-3, SMAD7に関しては一定の傾向は認められなかった。
  日本学術振興会, 若手研究, 北海道大学, 19K15991
• Development of realistic audiovisual educational system for veterinary clinical techniques with movable viewpoints videos
  Grants-in-Aid for Scientific Research
  29 Jun. 2018 - 31 Mar. 2021
  Satoshi Takagi
  Veterinary clinical education requires learning a variety of techniques, but unnecessary use of laboratory animals should be avoided as much as possible. For this reason, videos and alternative non-living animal models have been used; however, in order to provide more realistic feeling in practice, virtual reality (VR) materials were created and evaluated. The teaching materials were filmed with a 360-degree camera, played back in a dedicated mode using a smartphone application on a VR head mount, and viewed by veterinary students. As a result of the survey, 142 students reviewed the VR materials, with 78 students (53.1%) answering "very good", 51 students (34.7%) answering "good", and 13 students (8.8%) responding with "not good". VR teaching materials were considered useful to stimulate students' interest and motivation as an introduction to small animal clinical education.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Azabu University, 18K19256
• Evaluation of clinical usefulness of contrast-enhanced ultrasonography as an early diagnostic method to detect renal dysfunction in dogs
  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up
  31 Aug. 2012 - 31 Mar. 2014
  MORISHITA Keitaro
  Chronic kidney disease (CKD) is one of common disease in dogs and cats. However, current available renal functional tests have poor diagnostic sensitivity, so there is a need to establish a non-invassive method to predict renal damage earlier. The purpose of this study was to determine the feasibility using quantitative contrast-enhanced ultrasonography (CEUS) to detect changes in renal blood flow related to renal dysfunction.
  We firstly characterized the image enhancement of the normal canine kidney using 6 healthy dogs, and secondaly we investigated the feasibility of CEUS in dogs with CKD. Differences in perfusion parameters were present between the dogs with advanced CKD and healthy dogs, whereas no perfusion chenges were seen in dogs with early stage of CKD.
  Japan Society for the Promotion of Science, Grant-in-Aid for Research Activity Start-up, Hokkaido University, 24880004