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Yano-Nashimoto Saori
| Faculty of Veterinary Medicine Veterinary Medicine Basic Veterinary Sciences | Assistant Professor |
Researcher basic information
■ Degree■ URL
researchmap URLホームページURL■ Various IDs
Researcher number
- 00779548
Research Field■ Educational Organization
- Bachelor's degree program, School of Veterinary Medicine
- Doctoral (PhD) degree program, Graduate School of Veterinary Medicine
Research activity information
■ Awards■ Papers
- Male odor preference in female mice is modulated across reproductive stages via the posteroventral medial amygdala.
Satomi Komada; Katsura Kagawa; Ayaka Takimoto-Inose; Soichiro Yamaguchi; Saori Yano-Nashimoto
bioRxiv, 01 Apr. 2026, [Last author, Corresponding author], [International Magazine] - Transient changes in body weight and behavior during the placentation period in non-human primates and rodents
Saori Yano-Nashimoto; Kazutaka Shinozuka; Takuma Kurachi; Katsura Kagawa; Kentaro Q. Sakamoto; Yuko Shigeno; Kimie Niimi; Kumi O. Kuroda; Soichiro Yamaguchi
Scientific Reports, 16, 1, 8162, Springer Science and Business Media LLC, 25 Mar. 2026, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
Scientific journal, Abstract
During early pregnancy, many women experience physical changes, including nausea and vomiting of pregnancy (NVP), which negatively impact their quality of life. However, the absence of model animals has limited our understanding of such pregnancy-associated physiological changes. Here, we examined pregnancy-associated metabolic and behavioral changes in common marmosets and mice. Marmosets exhibited a transient weight decrease during the period of placental development in approximately 22% of pregnancies. Some marmosets repeatedly showed transient weight loss across multiple pregnancies, suggesting individual variations in the likelihood of pregnancy-associated weight loss. Although mice did not show apparent alteration in body weight, they exhibited a slowdown in food intake and alterations in locomotor activity during the corresponding phase. The observed transient changes in pregnant marmosets and mice may provide a basis for generating hypotheses regarding physiological changes associated with placentation. - Human chorionic gonadotropin inhibits locomotion but not food intake independently of the area postrema and the nucleus tractus solitarius in female mice
Masami Anan; Katsura Kagawa; Yuko Okamatsu-Ogura; Soichiro Yamaguchi; Saori Yano-Nashimoto
Physiology & Behavior, 303, 115151, 115151, Elsevier BV, Jan. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
Scientific journal - Functional expression of the proton channel OTOP1 in brown adipocytes
MD Mominul Islam; Omi Sasaki; Saori Yano-Nashimoto; Yuko Okamatsu-Ogura; Soichiro Yamaguchi
Biochemical and Biophysical Research Communications, Nov. 2025, [Peer-reviewed]
Scientific journal - Transmembrane channel-like 4 (TMC4) could act as a negative regulator of KCNQ1 (Kv7.1) potassium channel.
Hirota Aoyagi; Koya Kawaguchi; Saori Yano-Nashimoto; Soichiro Yamaguchi
Biochimica et biophysica acta. Biomembranes, 184460, 184460, 02 Oct. 2025, [Peer-reviewed], [International Magazine]
English, Scientific journal, TMC4 is a member of the transmembrane channel-like (TMC) protein family. In this family, TMC1 and TMC2 are thought to form the mechano-electrical transduction (MET) channel in the inner ear. On the other hand, the intrinsic functions of the other TMC family members (TMC3-8) are largely unknown. KCNQ1 (Kv7.1) channel is a voltage-gated potassium channel and plays crucial physiological roles with its auxiliary subunits, KCNE proteins (e.g. KCNQ1/KCNE1 complex contributes to cardiac repolarization, and KCNQ1/KCNE3 complex participates in epithelial ion transport). Recently, it was reported that TMC1 and TMC2 interacted with KCNQ1 and suppressed its K+ currents. However, the relationships between KCNQ1 and the other TMC proteins have not been examined. Here, we show a novel interaction and a functional association between overexpressed TMC4 and KCNQ1. The Bead Halo assay and FRET analysis revealed the physical interaction between these two proteins. Whole-cell patch clamp recording demonstrated that co-expression of TMC4 reduced KCNQ1 current densities without altering their voltage dependence and activation kinetics. This effect was also observed in the KCNQ1/KCNE1 and KCNQ1/KCNE3 channel complexes. A structural prediction using AlphaFold-Multimer suggested possible interaction sites between TMC4 and KCNQ1. Mutageneses, followed by patch clamp recording, suggested that specific amino acid residues at these sites contribute to the inhibitory effect of TMC4. These results indicate that TMC4 could function as a negative regulator of the KCNQ1 channel. Our findings could enhance the understanding of KCNQ1 channel regulation and propose potential research directions on the function of TMC4 under various physiological and pathological conditions. - Morning sickness-like changes during pregnancy in non-human primates and rodents
Saori Yano-Nashimoto; Kazutaka Shinozuka; Takuma Kurachi; Katsura Kagawa; Kentaro Q. Sakamoto; Yuko Shigeno; Kimie Niimi; Kumi O. Kuroda; Soichiro Yamaguchi
bioRxiv, 15 Aug. 2025, [Lead author, Corresponding author] - Behavioral competition between infant care and sexual behavior in male but not female common marmosets
Takuma Kurachi; Kazutaka Shinozuka; Chihiro Yoshihara; Saori Yano-Nashimoto; Ayako Y. Murayama; Junichi Hata; Hideyuki Okano; Atsuko Saito; Kumi O. Kuroda
Neuroscience Research, Jul. 2025, [Peer-reviewed], [Invited], ［Internationally co-authored］, [International Magazine]
Scientific journal - A proton channel, Otopetrin 1 (OTOP1) is N-glycosylated at two asparagine residues in third extracellular loop.
Omi Sasaki; Saori Yano-Nashimoto; Soichiro Yamaguchi
Journal of cellular physiology, 239, 9, e31403, Sep. 2024, [Peer-reviewed], [International Magazine]
English, Scientific journal, A proton (H+) channel, Otopetrin 1 (OTOP1) is an acid sensor in the sour taste receptor cells. Although OTOP1 is known to be activated by extracellular acid, no posttranslational modification of OTOP1 has been reported. As one of the posttranslational modifications, glycosylation is known to modulate many ion channels. In this study, we investigated whether OTOP1 is glycosylated and how the glycosylation affects OTOP1 function. Pharmacological and enzymatic examinations (using an N-glycosylation inhibitor, tunicamycin and peptide: N-glycanase F [PNGase F]) revealed that overexpressed mouse OTOP1 was N-glycosylated. As the N-glycans were Endoglycosidase H (Endo H)-sensitive, they were most likely high-mannose type. A site-directed mutagenesis approach revealed that both two asparagine residues (N238 and N251) in the third extracellular loop between the fifth transmembrane region and the sixth transmembrane region (L5-6) were the glycosylation sites. Prevention of the glycosylations by the mutations of the asparagine residues or by tunicamycin treatment diminished the whole-cell OTOP1 current densities. The results of cell surface biotinylation assay showed that the prevention of the glycosylations reduced the surface expression of OTOP1 at the plasma membrane. These results indicate that mouse OTOP1 is N-glycosylated at N238 and N251, and that the glycosylations are necessary for OTOP1 to show the maximum degree of H+ current densities at the plasma membrane through promoting its targeting to the plasma membrane. These findings on glycosylations of OTOP1 will be a part of a comprehensive understanding on the regulations of OTOP1 function. - Anxious about rejection, avoidant of neglect: Infant marmosets tune their attachment based on individual caregiver's parenting style.
Saori Yano-Nashimoto; Anna Truzzi; Kazutaka Shinozuka; Ayako Y Murayama; Takuma Kurachi; Keiko Moriya-Ito; Hironobu Tokuno; Eri Miyazawa; Gianluca Esposito; Hideyuki Okano; Katsuki Nakamura; Atsuko Saito; Kumi O Kuroda
Communications biology, 7, 1, 212, 212, 20 Feb. 2024, [Peer-reviewed], [Lead author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Children's secure attachment with their primary caregivers is crucial for physical, cognitive, and emotional maturation. Yet, the causal links between specific parenting behaviors and infant attachment patterns are not fully understood. Here we report infant attachment in New World monkeys common marmosets, characterized by shared infant care among parents and older siblings and complex vocal communications. By integrating natural variations in parenting styles and subsecond-scale microanalyses of dyadic vocal and physical interactions, we demonstrate that marmoset infants signal their needs through context-dependent call use and selective approaches toward familiar caregivers. The infant attachment behaviors are tuned to each caregiver's parenting style; infants use negative calls when carried by rejecting caregivers and selectively avoid neglectful and rejecting caregivers. Family-deprived infants fail to develop such adaptive uses of attachment behaviors. With these similarities with humans, marmosets offer a promising model for investigating the biological mechanisms of attachment security. - Atypical isn't necessarily a disadvantage
SAORI YANO-NASHIMOTO
Japanese Journal of Animal Psychology, 74, 1, 27, 28, Japanese Society of Animal Psychology, 2024, [Peer-reviewed], [Invited], [Lead author, Corresponding author], [Domestic magazines] - Distinct roles of amylin and oxytocin signaling in intrafamilial social behaviors at the medial preoptic area of common marmosets.
Takuma Kurachi; Kazutaka Shinozuka; Chihiro Yoshihara; Saori Yano-Nashimoto; Ayako Y Murayama; Junichi Hata; Yawara Haga; Hideyuki Okano; Kumi O Kuroda
Communications biology, 6, 1, 1231, 1231, 05 Dec. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Calcitonin receptor (Calcr) and its brain ligand amylin in the medial preoptic area (MPOA) are found to be critically involved in infant care and social contact behaviors in mice. In primates, however, the evidence is limited to an excitotoxic lesion study of the Calcr-expressing MPOA subregion (cMPOA) in a family-living primate species, the common marmoset. The present study utilized pharmacological manipulations of the cMPOA and shows that reversible inactivation of the cMPOA abolishes infant-care behaviors in sibling marmosets without affecting other social or non-social behaviors. Amylin-expressing neurons in the marmoset MPOA are distributed in the vicinity of oxytocin neurons in the anterior paraventricular nucleus of the hypothalamus. While amylin infusion facilitates infant carrying selectively, an oxytocin's inverse agonist, atosiban, reduces physical contact with non-infant family members without grossly affecting infant care. These data suggest that the amylin and oxytocin signaling mediate intrafamilial social interactions in a complementary manner in marmosets. - Cibacron blue 3G-A is a novel inhibitor of Otopetrin 1 (OTOP1), a proton channel.
Md Mominul Islam; Omi Sasaki; Saori Yano-Nashimoto; Yuko Okamatsu-Ogura; Soichiro Yamaguchi
Biochemical and biophysical research communications, 665, 64, 70, 12 Jul. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Otopetrin 1 (OTOP1) is a proton (H+) channel which detects acidic stimuli in sour taste receptor cells and plays some sort of role in the formation of otoconia in the inner ear. Although it is known that zinc ion (Zn2+) inhibits OTOP1, Zn2+ requires high concentrations (mM order) to inhibit OTOP1 sufficiently, and no other inhibitors have been found. Therefore, to identify a novel inhibitor, we screened a chemical library (LOPAC1280) by whole-cell patch clamp recordings, measuring proton currents of heterologously-expressed mouse OTOP1. From the screening, we found that reactive blue 2 inhibited OTOP1 currents. Further evaluations of three analogues of reactive blue 2 revealed that cibacron blue 3G-A potently inhibited OTOP1 currents. Cibacron blue 3G-A inhibited OTOP1 currents in a concentration-dependent manner, and its 50% inhibitory concentration (IC50) and the Hill coefficient were 5.0 μM and 1.1, respectively. The inhibition of OTOP1 currents by cibacron blue 3G-A was less affected by extracellular anion compositions, membrane potentials, and low pH than the inhibition by Zn2+. These results suggest that the inhibition of OTOP1 by cibacron blue 3G-A is neither likely to be a pore-blocking inhibition nor a competitive inhibition. Furthermore, our findings revealed that cibacron blue 3G-A can be used as a novel inhibitor of OTOP1 especially under the conditions in which OTOP1 activity is evaluated such as low pH. - Neurobiology of parent-infant relationships in rodents and primates
SAORI YANO-NASHIMOTO; KUMI O. KURODA
Japanese Journal of Animal Psychology, 73, 2, 35, 49, Japanese Society of Animal Psychology, 2023, [Peer-reviewed], [Lead author]
Scientific journal - A calcitonin receptor-expressing subregion of the medial preoptic area is involved in alloparental tolerance in common marmosets.
Kazutaka Shinozuka; Saori Yano-Nashimoto; Chihiro Yoshihara; Kenichi Tokita; Takuma Kurachi; Ryosuke Matsui; Dai Watanabe; Ken-Ichi Inoue; Masahiko Takada; Keiko Moriya-Ito; Hironobu Tokuno; Michael Numan; Atsuko Saito; Kumi O Kuroda
Communications biology, 5, 1, 1243, 1243, 21 Nov. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Like humans, common marmoset monkeys utilize family cooperation for infant care, but the neural mechanisms underlying primate parental behaviors remain largely unknown. We investigated infant care behaviors of captive marmosets in family settings and caregiver-infant dyadic situations. Marmoset caregivers exhibited individual variations in parenting styles, comprised of sensitivity and tolerance toward infants, consistently across infants, social contexts and multiple births. Seeking the neural basis of these parenting styles, we demonstrated that the calcitonin receptor-expressing neurons in the marmoset medial preoptic area (MPOA) were transcriptionally activated during infant care, as in laboratory mice. Further, site-specific neurotoxic lesions of this MPOA subregion, termed the cMPOA, significantly reduced alloparental tolerance and total infant carrying, while sparing general health and other social or nonsocial behaviors. These results suggest that the molecularly-defined neural site cMPOA is responsible for mammalian parenting, thus provide an invaluable model to study the neural basis of parenting styles in primates. - 動物の世界の愛着
矢野(梨本)沙織; 篠塚一貴; 吉田さちね; 黒田公美
チャイルドヘルス, 22, 2, 111, 113, 診断と治療社, Feb. 2019, [Lead author]
Japanese - 攻撃性の脳内基盤Ⅱ－最近の基礎研究の動向
Kazutaka Shinozuka; Saori Yano-Nashimoto; Menno R. Kruk; Kumi O. Kuroda
Neurobiology and clinical research on aggression, 40, 9, 1067, 1076, 2017
Japanese, Scientific journal - Female Mice Avoid Male Odor from the Same Strain via the Vomeronasal System in an Estrogen-Dependent Manner
Saori Yano; Kentaro Q. Sakamoto; Yoshiaki Habara
CHEMICAL SENSES, 40, 9, 641, 648, Nov. 2015, [Peer-reviewed]
English, Scientific journal - Estrus Cycle-Related Preference of BALB/c Female Mice for C57BL/6 Males Is Induced by Estrogen
Saori Yano; Kentaro Q. Sakamoto; Yoshiaki Habara
JOURNAL OF VETERINARY MEDICAL SCIENCE, 74, 10, 1311, 1314, Oct. 2012, [Peer-reviewed]
English, Scientific journal
- Full activity of Otopetrin-1 on the plasma membrane requires N-glycosylations at two sites in the third extracellular loop
佐々木雄望; 矢野(梨本)沙織; 山口聡一郎, 日本解剖学会総会・全国学術集会抄録集(CD-ROM), 130th, 2025 - Transmembrane channel-likeタンパク質との相互作用が電位依存性K+チャネルKCNQ1の機能に及ぼす影響の評価
青柳博太; 川口光哉; 矢野(梨本)沙織; 山口聡一郎, 日本獣医学会学術集会講演要旨集, 167th, 2024 - 霊長類マーモセットの子の愛着:親の養育からの影響と関係脳部位の探索
黒田公美; 倉地卓将; 矢野(梨本)沙織, 日本生物学的精神医学会(Web), 46th, 2024 - プロトン(H+)チャネルOTOP1の新規阻害薬の発見
イスラム モハメドモミヌル; 佐々木雄望; 矢野沙織; 岡松優子; 山口聡一郎, 日本獣医学会学術集会講演要旨集, 166th, 2023 - プロトン(H+)チャネルOTOP1における糖鎖付加の検討
佐々木雄望; 矢野沙織; 山口聡一郎, 日本獣医学会学術集会講演要旨集, 166th, 2023 - 子育ての分子神経回路:雌雄で共通するコアメカニズムと雌雄差のある制御機構
黒田公美; 黒田公美; 矢野(梨本)沙織; 倉地卓将; 篠塚一貴; 吉原(根本)千尋, 日本獣医学会学術集会講演要旨集, 166th, 2023 - 哺乳類の親の子育てスタイルの神経機構と子の愛着形成に与える影響
黒田公美; 篠塚一貴; 矢野沙織; 斎藤慈子; 吉原千尋; TRUZZI Anna; 村山綾子; 倉地卓将, 日本赤ちゃん学会学術集会プログラム・要旨集, 22nd (CD-ROM), 2022 - 牛耳下腺腺房細胞の管腔側膜にCl-/HCO3-交換輸送体であるSLC26A6が機能発現する
井上龍生; 山口聡一郎; 矢野(梨本)沙織, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 - Parental behavior in common marmosets: consideration from multi indicators
SHINOZUKA Kazutaka; YANO-NASHIMOTO Saori; SHINDO Sayaka; SAITO Atsuko; KURODA Kumi, Primate Research Supplement, 34, 0, 66, 66, 2018コモンマーモセットは，ヒトと同様に共同繁殖をする種であり，母親だけでなく，父親や兄姉個体も子育てに参加することから，霊長類の養育行動の神経メカニズムを調べるよいモデルとなる。本発表では，家族で飼育されているマーモセットのホームケージ内での観察，および乳児回収テスト，食物分配テストという実験場面での行動といった複数の指標による養育行動のデータを示す。【ホームケージ内での背負い行動】子が背負われている確率は生後35日頃までは50%以上あり，その後急速に減衰した。これは背負う個体の頭数によって変わらず，背負い行動が家族内で柔軟に分担されていることが示唆された。子を背負っている個体は，移動，食物摂取，遊び行動が減少したことから，背負い行動は個体にとってコストであるといえる。一方，子を背負っている個体は他個体からグルーミングを受ける確率が高くなる傾向がみられ，グルーミングが背負い行動に対する報酬となっていることが示唆された。【乳児回収テスト】養育個体が子を回収するまでの潜時は，分離による子の発声に対する感受性や，子を回収するために必要なスキルを表し，回収後に子を拒絶せずに背負い続けることは，子（背負い）に対する寛容性を表すと考えられる。養育経験は生後早期の回収潜時を減少させたが，子に対する寛容性には影響を与えなかった。【食物分配実験】養育個体が獲得した餌を子が取ることへの寛容さを計測するもので，家族ごとに大きな差がみられたが，背負い行動と食物分配行動の間には弱い相関関係が示唆された。【子育て行動と性行動の関係】マーモセットは出産後1週間で排卵し，連続妊娠することがあるが，連続妊娠で出産した場合，その前の出産後1週間時点の父親の背負い行動が減少していた。父親の子育て行動と性行動の間には，トレードオフがあることが示唆された。このように養育行動の様々な側面を測定することで，養育行動の発現メカニズムの詳細を明らかにできると考えられる。
, Primate Society of Japan, Japanese
- 生命科学特論, 2024年, 博士後期課程, 獣医学院
- 先端生命科学特論Ⅱ：細胞生理学, 2024年, 博士後期課程, 獣医学院
- 生物統計学演習, 2024年, 学士課程, 獣医学部
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■ Research Themes
- マウスを用いたつわり脳内機構の探求―エストロゲンによるGDF15作用増強
科学研究費助成事業
01 Apr. 2026 - 31 Mar. 2029
梨本 沙織
日本学術振興会, 基盤研究(C), 北海道大学, 26K12279 - マウスモデルを用いたhCGのつわり関連症状との因果関係検証および作用脳部位・神経群特定
内藤記念女性研究者研究助成金
Dec. 2024 - Sep. 2028
矢野(梨本)沙織
公益財団法人内藤記念科学振興財団 - 哺乳類の生理学・心理学・行動学から共同養育の本質を捉える ―少子化問題の解決に向けて
北海道大学 J-PEAKS 連携研究プラットフォーム事業 インキュベーションステージ
Apr. 2025 - Mar. 2027
瀧本彩加; 矢野(梨本)沙織; 河合正人
Coinvestigator - マウスのつわり様症状とGDF15,hCGの因果関係検証及び作用脳部位・神経群探索
科学研究費助成事業
01 Apr. 2023 - 31 Mar. 2026
梨本 沙織
日本学術振興会, 若手研究, 北海道大学, 23K15797 - Investigation of brain regions regulating pair bond in primates: analysis by electrical stimulation and pharmacogenetic techniques
Grants-in-Aid for Scientific Research
01 Apr. 2019 - 31 Mar. 2023
Yano-Nashimoto Saori
The ultimate goal of this research is to elucidate the brain mechanisms of family bond. Although our initial target was pair bond, we decided to focus on infant attachment because we occasionally found some marmosets show atypical behavior similar to human attachment disorder.
We revealed that infant marmoset modified their attachment behavior according to the parenting style of each caregiver. On the other hand, artificially reared infants could not develop the adaptive use of attachment behaviors or age-appropriate autonomy. In addition, the received care in childhood affected later sociality and caused paradoxical negative responses towards social and non-social rewards. These characteristics were similar to those in human, indicating that a marmoset is a useful animal model for attachment research.
Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, 19K16901 - Identification of brain area required for rat attachment behavior to their mother
Grants-in-Aid for Scientific Research
01 Apr. 2016 - 31 Mar. 2019
Ohmura Nami; KURODA kumi; YANO saori; OKUMA lana
We focused on three representative behaviors of rat’s filial attachment behaviors: maternal contact, suckling and Transport Response. We confirmed that pup’s calm down, stop crying and that their heart rate decrease during these attachment behaviors.
To narrow down the related the brain regions related to these attachment behaviors and responses, we decerebrated preweaning rat pups with a razor blade, at either a coronal whole plane or partial plane. The effects of decerebration on the pup’s behaviors were evaluated. Results suggest that filial attachment behaviors may be regulated by several brain areas, and that the lateral-ventral area of the forebrain may be related to maternal contact, suckling, and calming down component seen in Transport Response.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Institute of Physical and Chemical Research, Competitive research funding, 16K21617
