Researcher basic information

• PhD(Veterinary Medicine), Hokkaido University
• Master of Veterinary Medical Science, Hokkaido University

• https://researchmap.jp/read0180169?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901022044782571

• 200901022044782571

• Chronic inflammation
• 自己免疫疾患
• 超音波診断
• 画像診断
• Veterinary Diagnostic imaging
• Autoimmune Diseases
• Diagnostic Ultrasound
• Diagnostic imaging

• Life Science, Radiological sciences
• Life Science, Connective tissue disease and allergy
• Life Science, Veterinary medical science

• Bachelor's degree program, School of Veterinary Medicine
• Doctoral (PhD) degree program, Graduate School of Veterinary Medicine

Research activity information

• 2004, 日本小動物獣医学会北海道獣医師会長賞
  Japan
• 1999, 平成11年度日本小動物獣医学会(北海道)獣医師会長賞
  Japan
• 1999, 日本獣医学会大会長賞 獣医学奨励賞
  Japan
• 1998, 日本獣医学会大会長賞 獣医学奨励賞
  Japan
• 1997, 平成9年度日本小動物獣医学会(北海道)地区学会長賞
  Japan

• Deep-learning-based automatic liver segmentation using computed tomography images in dogs
  Seungyeon Lee; Genya Shimbo; Nozomu Yokoyama; Kensuke Nakamura; Ren Togo; Takahiro Ogawa; Miki Haseyama; Mitsuyoshi Takiguchi
  Frontiers in Veterinary Science, 12, Frontiers Media SA, 21 Oct. 2025, [Peer-reviewed], [Corresponding author]
  English, Scientific journal, Introduction
  
  Deep learning-based automated segmentation has significantly improved the efficiency and accuracy of human medicine applications. However, veterinary applications, particularly canine liver segmentation, remain limited. This study aimed to develop and validate a deep learning model based on a 3D U-Net architecture for automated liver segmentation in canine abdominal computed tomography (CT) scans.
  
  Methods
  
  A total of 221 canine abdominal CT scans were analyzed, comprising 159 cases without hepatic masses and 62 cases with hepatic masses. The model was trained and evaluated using two separate datasets: one containing cases without hepatic masses (Experiment 1) and the other combining cases with and without hepatic masses (Experiment 2).
  
  Results
  
  Both experiments demonstrated high segmentation performance, achieving mean Dice similarity coefficients of 0.926 (Experiment 1) and 0.929 (Experiment 2).
  
  Discussion
  
  The manual and predicted liver volumes showed excellent agreement, highlighting the potential clinical applicability of this approach.
• Potential human health risk of carbapenem-non-susceptible Pseudomonas aeruginosa from companion animals.
  Jirachaya Toyting-Hiraishi; Toyotaka Sato; Mana Tohyama; Taro Fujino; Kaho Okada; Kazuyoshi Sasaoka; Nozomu Yokoyama; Kana Torii; Akio Suzuki; Yuzo Tsuyuki; Kensuke Nakamura; Mitsuyoshi Takiguchi; Motohiro Horiuchi
  The Journal of antimicrobial chemotherapy, 19 Sep. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND AND OBJECTIVES: The close bond between companion animals and humans may accelerate the spread of antimicrobial-resistant bacteria. Pseudomonas aeruginosa, an opportunistic pathogen in both, poses a public health threat due to antimicrobial resistance (AMR) and diverse virulence factors. However, One Health-based comparison remains limited. This study investigated the current AMR status and molecular characteristics of P. aeruginosa in companion animals in Japan to assess potential human health risks. METHODS: We examined 197 P. aeruginosa clinical isolates from companion animals [dogs (n = 99) and cats (n = 98)] across Japan in 2024. Antimicrobial susceptibility to human clinical antibiotics was evaluated. In carbapenem-non-susceptible isolates, multilocus sequence typing and detection of resistance genes and virulence factors were performed. RESULTS: Ciprofloxacin (20.3%) and piperacillin (10.7%) showed the highest resistance rates, with 5.6% of isolates being multidrug-resistant. Carbapenem resistance rates were 6.1% for imipenem and 1.0% for meropenem. Thirty-five isolates (17.8%) exhibited carbapenem non-susceptibility but remained susceptible to cefepime, ciprofloxacin, or amikacin. Of 27 identified sequence types, 20 (77.1% of carbapenem-non-susceptible isolates) were known in humans, including two high-risk clones (ST233 and ST298; 8.6%) reported for the first time in Japanese companion animals. These isolates carried mutations in efflux pump-related genes and multiple virulence factors. One showed close genetic relatedness to a human isolate, suggesting possible interspecies transmission. CONCLUSIONS: Our findings highlight the potential cross-species transmission risk of antimicrobial-resistant P. aeruginosa. Identification of shared high-risk clones with multiple virulence factors emphasizes the need for continuous vigilance and actions within the One Health framework.
• Outcomes and Prognostic Factors in Dogs With Presumed Intracranial Gliomas Treated With Definitive-Intent Intensity Modulated Radiation Therapy: 55 Cases (2014-2023).
  Yohichi Fukuyama; Kenji Hosoya; Sangho Kim; Koangyong Sung; Tatsuya Deguchi; Genya Shimbo; Kazuyoshi Sasaoka; Ryouhei Kinoshita; Mitsuyoshi Takiguchi
  Veterinary and comparative oncology, 25 Aug. 2025, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, Radiation therapy (RT) is the treatment of choice for canine intracranial gliomas. Recently, modern advanced radiation techniques, including intensity modulated RT (IMRT) and volumetric modulated arc therapy (VMAT), have become widely available in veterinary medicine. However, the glioma-specific therapeutic outcomes of patients treated with modern RT remain unclear. This study aimed to describe survival outcomes and tumour response and to identify whether any treatment, clinical, and imaging factors were predictive of prognosis in dogs with intracranial gliomas treated with definitive-intent IMRT alone. Medical records of dogs with presumed intracranial gliomas that underwent definitive-intent IMRT were retrospectively reviewed. Fifty-five dogs were included. Amongst them, 29 and 26 underwent fractionated RT (FRT) and stereotactic RT (SRT), respectively. In the 44 dogs that underwent follow-up magnetic resonance imaging (MRI), the overall measurable response rate was 77.3%. Clinical improvement was observed in 92% of the dogs. Local tumour regrowth and drop metastases were observed in 17 (30.9%) and 10 dogs (18.2%), respectively. The median overall survival, disease-specific survival, and progression-free survival were 432, 670, and 441 days, respectively. Seven dogs (12.7%) died during RT or within 6 weeks. There was no statistically significant difference in the survival times between FRT and SRT. In the multivariate analysis, poor performance status, tumour location in the diencephalon, and fluid-attenuated inversion recovery heterogeneity were significantly associated with shorter survival times. These findings suggest that definitive-intent RT results in tumour shrinkage and prolonged survival (432 days) with minimal radiation toxicity regardless of the RT protocol used. Performance status and MRI findings can be useful for predicting prognosis.
• Effects of cardiac volume loading on left atrial stiffness estimated by 2-dimensional echocardiography in healthy dogs.
  Tatsuyuki Osuga; Masahiro Tamura; Noboru Sasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 1, 7, 14 Aug. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVE: To elucidate the effect of cardiac volume loading, which should physiologically increase left atrial (LA) stiffness (LASt), on echocardiographically estimated LASt in healthy dogs. METHODS: 6 healthy Beagle dogs were anesthetized. In each dog, a Swan-Ganz catheter was placed to measure the mean pulmonary capillary wedge pressure. Cardiac volume loading was performed by IV infusion with hydroxyethylated starch solution. Echocardiography was transthoracically conducted at the baseline and at mean pulmonary capillary wedge pressures of 10 to 15, 15 to 20, 20 to 25, and 25 to 30 mm Hg. The echocardiographic indicators of LA pressure, including the peak velocity of early diastolic transmitral flow (E), ratio of E to the isovolumic relaxation time (E/IVRT), ratio of E to the peak velocity of early diastolic mitral annular motion (E/E'), and LA reservoir strain, were determined to estimate LASt. RESULTS: During cardiac volume loading, E, E/IVRT, and LA reservoir strain were increased, and E/E' was decreased, when compared with the baseline. Consequently, LASts estimated by using E, E/IVRT, and E/E' were decreased or remained unchanged during cardiac volume loading. CONCLUSIONS: Echocardiographically estimated LASt may not be increased by cardiac volume loading in healthy dogs. CLINICAL RELEVANCE: Healthy dogs may not be an appropriate model to elucidate the effect of cardiac volume loading on echocardiographically estimated LASt. This is problematic, especially for dogs with myxomatous mitral valve disease that are older and may have cardiac dysfunction.
• Ultrasonographic features and blood biochemistry findings of cholangiocarcinoma in a captive polar bear (Ursus maritimus)
  Keita Kakisaka; Nozomu Yokoyama; Fuka Takahashi; Keisuke Aoshima; Mitsuyoshi Takiguchi
  Veterinary Record Case Reports, Wiley, 28 Jun. 2025, [Peer-reviewed], [Corresponding author]
  English, Scientific journal, ABSTRACT
  
  A 29‐year‐old male captive polar bear (Ursus maritimus) with a year‐long history of elevated gamma‐glutamyl transpeptidase (GGT) presented with a 2‐week history of anorexia and abdominal distention. Blood biochemistry revealed a severely elevated GGT (>1500 IU/L). Abdominal ultrasound revealed multiple cystic hepatic masses and marked ascites. Fourteen days after initial presentation, the patient was found dead. Postmortem examination revealed hepatomegaly with diffuse, multifocal, coalescing cysts containing a red‒tan to dark‐red serous fluid. Histopathologically, the cyst walls were lined by flattened or cuboidal neoplastic cells, some exhibiting tubulopapillary projections into the lumen. Metastatic lesions were identified in the anterior mediastinal lymph nodes, lungs and kidneys. This case was diagnosed as cholangiocarcinoma. While there are numerous reports of cholangiocarcinoma in polar bears, published data on blood biochemistry and abdominal ultrasonographic findings are limited. This case report illustrates the benefit of blood biochemistry and abdominal ultrasound in investigating cholangiocarcinoma in polar bears.
• Evaluation of the course of improvement with molnupiravir treatment for feline infectious peritonitis.
  Shino Yoshida; Mei Sugawara-Suda; Kazuyoshi Sasaoka; Noboru Sasaki; Nozomu Yokoyama; Kensuke Nakamura; Keitaro Morishita; Sangho Kim; Takafumi Sunaga; Mitsuyoshi Takiguchi
  The Canadian veterinary journal = La revue veterinaire canadienne, 66, 5, 546, 554, May 2025, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, OBJECTIVE: To clarify the clinical course during molnupiravir treatment for feline infectious peritonitis (FIP). ANIMALS AND PROCEDURE: Cats diagnosed with FIP and treated with molnupiravir at Hokkaido University Veterinary Teaching Hospital (Sapporo, Hokkaido, Japan) were retrospectively reviewed. RESULTS: Eleven cats were eligible for inclusion. Six cats had effusive FIP and 5 had non-effusive FIP. In noneffusive cases, 2 cats had neurological abnormalities at diagnosis, whereas 1 additional cat developed neurological signs during treatment. The median initial dosage of molnupiravir was 13.0 mg/kg (range: 10.0 to 15.0 mg/kg), PO, q12h. One cat died after 11 d and the remaining 10 cats completed an 84-day course of treatment. All neurological cases were given dosage increases, extended treatment duration, or both. The median final dosage of molnupiravir in non-neuro-FIP cases was 13.1 mg/kg (range: 10.0 to 15.0 mg/kg), PO, q12h, whereas dosages in neuro-FIP cases were 15.0, 15.2, and 17.2 mg/kg, PO, q12h in the 3 affected cats, respectively. In non-neurological cases, dysrexia, lethargy, and high serum amyloid A were resolved within 15 d. Total follow-up duration ranged from 175 to 362 d. No relapses were observed. CONCLUSION AND CLINICAL RELEVANCE: Monitoring responses to molnupiravir treatment requires observing clinical signs and conducting clinicopathological evaluations, including acute-phase protein evaluation.
• Pre-operative left atrial size and functions are predictors of left atrial reverse remodelling after mitral valvuloplasty for myxomatous mitral valve disease in dogs.
  S Kawamoto; K Nakamura; T Mori; T Wada; A Fujiwara; M Takiguchi
  Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology, 57, 47, 57, Feb. 2025, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, OBJECTIVES: The objective of this study was to determine alterations in left atrial size, function, and determinants of left atrial reverse remodelling (LARR) after mitral valvuloplasty (MVP) in dogs with myxomatous mitral valve disease. ANIMALS: Thirty-seven dogs undergoing MVP were included in this study. MATERIALS AND METHODS: Echocardiographic parameters, including left atrial (LA) strain, were evaluated at baseline, during the early postoperative period (within two weeks), and at one and three months postoperatively. Patients were divided into two groups based on the attainment of LARR, defined as a left atrial-to-aortic root ratio (LA:Ao) < 1.6 at three months. The predictors of LARR were analysed using univariate logistic regression analysis. RESULTS: Left atrial-to-aortic root ratio significantly decreased in the early postoperative period (median: 1.68; lower and upper quartiles: 1.54-1.92) compared to that in baseline (2.41; 2.00-2.80), with a further significant reduction observed at three months (1.46; 1.34-1.63). Left atrial reverse remodelling was achieved in 27 (73%) patients. Baseline left atrial-to-aortic root ratio was higher, and peak atrial longitudinal strain and peak atrial contraction strain (PACS) were lower in the non-LARR group than in the LARR group. Among the baseline parameters, PACS was the strongest predictor of LARR (area under the curve: 0.837). CONCLUSIONS: Following MVP, the LA size decreased in the early postoperative period and continued to decrease over three months of follow-up. A low PACS score was the most reliable predictor of inadequate LARR. Further studies are necessary to determine the relationship between LARR, prognosis, and the optimal timing for surgical intervention.
• Reference intervals for urinary metanephrines to creatinine ratios in dogs in Japan.
  Noriyuki Nagata; Yoshinori Ikenaka; Hiryu Sawamura; Noboru Sasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 03 Sep. 2024, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal, The urinary normetanephrine to creatinine ratio (uNMN/Cr) and urinary metanephrine to creatinine ratio (uMN/Cr) are commonly evaluated for the diagnosis of pheochromocytomas (PCC) in dogs. This study aimed to establish reference intervals for uNMN/Cr and uMN/Cr in 56 healthy dogs in Japan and to investigate the effect of urine collection methods on these measurements in 15 non-PCC dogs. The upper limits of reference intervals for uNMN/Cr and uMN/Cr were 124.4 nmol/mmol (90% confidence interval [CI] 107.7-137.0) and 121.1 nmol/mmol (90% CI 102.8-145.1), respectively. Both uNMN/Cr and uMN/Cr were significantly higher when urine was collected in the hospital compared to at home. Several factors, including the method of urine collection, should be considered when utilizing these reference intervals.
• Case report: Echinococcus multilocularis infection in a dog showing gastrointestinal signs in Hokkaido, Japan
  Izumi Kida; Naoki Hayashi; Nozomu Yokoyama; Noriyuki Nagata; Kazuyoshi Sasaoka; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Hirokazu Kouguchi; Kinpei Yagi; Ryo Nakao; Mitsuyoshi Takiguchi; Nariaki Nonaka
  Frontiers in Veterinary Science, 11, Frontiers Media SA, 13 Jun. 2024, [Peer-reviewed]
  English, Scientific journal, Echinococcus multilocularis is a cestode that causes human alveolar echinococcosis, a lethal zoonotic disease distributed in the northern hemisphere. The life cycle of this parasite is maintained in nature by voles as intermediate hosts and foxes as definitive hosts in Hokkaido, Japan. Although dogs are also susceptible to the parasite, the infection has been considered typically asymptomatic. We report the detection of E. multilocularis eggs in the diarrheal feces of a dog with chronic gastrointestinal signs, which disappeared after anthelmintic treatment. The mitochondrial genome sequence constructed by sequencing of the overlapping PCRs using DNA from the eggs was identical to the most predominant haplotype previously reported in red foxes in Hokkaido. This case highlights that Echinococcus infection should be considered as a differential diagnosis for diarrheal dogs in the disease endemic areas. Further efforts are needed to accumulate parasite genotypes in domestic dogs as well as humans to assess the risk of human infection from dogs.
• Urinary steroid profiling using liquid chromatography-tandem mass spectrometry for the diagnosis of canine Cushing's syndrome.
  N Nagata; H Sawamura; Y Ikenaka; K Morishita; K Hosoya; N Sasaki; K Nakamura; M Takiguchi
  Veterinary journal (London, England : 1997), 106151, 106151, 29 May 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Serum cortisol measurements by chemiluminescence enzyme immunoassay (CLEIA) are widely used to diagnose hypercortisolism (HC) or Cushing's syndrome in dogs. However, they are associated with problems such as the need for multiple blood collections under stressful conditions or cross-reactivity between hormones. Therefore, a less invasive and more accurate diagnostic method is required. This study aimed to develop a urinary steroid profile analysis method using liquid chromatography-tandem mass spectrometry (LC/MS/MS) and to evaluate its clinical usefulness. Sixty-five healthy dogs and 38 dogs with suspected HC were included in the study. Using LC/MS/MS, the levels of 11 steroid hormones in the urine were determined. We established the upper limit of the reference interval for each urinary steroid-to-creatinine ratio and evaluated their diagnostic performances. The levels of the five steroid hormones were significantly higher in the 14 dogs with HC than in the 24 dogs with mimicking HC and 65 healthy dogs. The urinary corticosterone-to-creatinine ratio showed the highest diagnostic accuracy (area under the curve, 0.96). A significant correlation was seen between urinary cortisol concentrations measured by LC/MS/MS and CLEIA (rs = 0.88, P <0.001), although the CLEIA measurements were significantly higher than the LC/MS/MS measurements (P <0.001). LC/MS/MS-based urinary steroid profiles are a promising tool for diagnosing canine HC.
• Antimicrobial resistance and self-reported hand hygiene awareness before and after an infection prevention and control programme: a 7-year analysis in a small animal veterinary teaching hospital
  K. Sasaoka; T. Sato; K. Morishita; K. Hosoya; N. Yokoyama; T. Sato; M. Horiuchi; M. Takiguchi
  The Veterinary Journal, 106154, 106154, Elsevier BV, May 2024, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• A de novo nonsense variant in the DMD gene associated with X-linked dystrophin-deficient muscular dystrophy in a cat.
  Nozomu Yokoyama; Yuki Matsumoto; Takahisa Yamaguchi; Kazuki Okada; Ryohei Kinoshita; Genya Shimbo; Hisashi Ukawa; Ryuga Ishii; Kensuke Nakamura; Jumpei Yamazaki; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 13 Apr. 2024, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, BACKGROUND: X-linked dystrophin-deficient muscular dystrophy (MD) is a form of MD caused by variants in the DMD gene. It is a fatal disease characterized by progressive weakness and degeneration of skeletal muscles. HYPOTHESIS/OBJECTIVES: Identify deleterious genetic variants in DMD by whole-genome sequencing (WGS) using a next-generation sequencer. ANIMALS: One MD-affected cat, its parents, and 354 cats from a breeding colony. METHODS: We compared the WGS data of the affected cat with data available in the National Center for Biotechnology Information database and searched for candidate high-impact variants by in silico analyses. Next, we confirmed the candidate variants by Sanger sequencing using samples from the parents and cats from the breeding colony. We used 2 genome assemblies, the standard felCat9 (from an Abyssinian cat) and the novel AnAms1.0 (from an American Shorthair cat), to evaluate genome assembly differences. RESULTS: We found 2 novel high-impact variants: a 1-bp deletion in felCat9 and an identical nonsense variant in felCat9 and AnAms1.0. Whole genome and Sanger sequencing validation showed that the deletion in felCat9 was a false positive because of misassembly. Among the 357 cats, the nonsense variant was only found in the affected cat, which indicated it was a de novo variant. CONCLUSION AND CLINICAL IMPORTANCE: We identified a de novo variant in the affected cat and next-generation sequencing-based genotyping of the whole DMD gene was determined to be necessary for affected cats because the parents of the affected cat did not have the risk variant.
• Interventricular inflow time difference assessed by dual pulsed-wave Doppler echocardiography in dogs with myxomatous mitral valve disease
  T. Morita; K. Nakamura; T. Osuga; S. Kawamoto; M. Takiguchi
  Journal of Veterinary Cardiology, 51, 24, 34, Elsevier BV, Feb. 2024, [Peer-reviewed], [Last author]
  English, Scientific journal
• Use of genome-wide DNA methylation analysis to identify prognostic CpG site markers associated with longer survival time in dogs with multicentric high-grade B-cell lymphoma.
  Yong Bin Teoh; Teita Ishizaki; Yumiko Kagawa; Shoko Yokoyama; Jaroslav Jelinek; Yuki Matsumoto; Hirotaka Tomiyasu; Hajime Tsujimoto; Mitsuyoshi Takiguchi; Jumpei Yamazaki
  Journal of veterinary internal medicine, 38, 1, 316, 325, 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: DNA methylation analysis might identify prognostic CpG sites in CHOP-treated dogs with multicentric high-grade B-cell lymphoma (MHGL) with heterogenous prognosis. OBJECTIVE: To identify prognostic CpG sites of MHGL through genome-wide DNA methylation analysis with pyrosequencing validation. ANIMALS: Test group: 24 dogs. Validation group: 100 dogs. All client-owned dogs were diagnosed with MHGL and treated with CHOP chemotherapy. METHODS: Cohort study. DNA was extracted from lymph node samples obtained via FNA. Genome-wide DNA methylation analysis using Digital Restriction Enzyme Analysis of Methylation (DREAM) was performed on the test group to identify differentially methylated CpG sites (DMCs). Bisulfite pyrosequencing was used to measure methylation status of candidate DMCs in the validation group. Median survival times (MST) were analyzed using Kaplan-Meier (log-rank) product limit method. RESULTS: DREAM analyzed 101 576 CpG sites. Hierarchical clustering of 16 262 CpG sites in test group identified group with better prognosis (MST = 55-477 days vs 10-301 days, P = .007). Volcano plot identified 1371 differentially methylated CpG sites (DMCs). DMC near the genes of FAM213A (DMC-F) and PHLPP1 (DMC-P) were selected as candidates. Bisulfite-pyrosequencing performed on validation group showed group with methylation level of DMC-F < 40% had favorable prognosis (MST = 11-1072 days vs 8-1792 days, P = .01), whereas group with the methylation level combination of DMC-F < 40% plus DMC-P < 10% had excellent prognosis (MST = 18-1072 days vs 8-1792 days, P = .009). CONCLUSION AND CLINICAL IMPORTANCE: Methylation status of prognostic CpG sites delineate canine MGHL cases with longer MST, providing owners with information on expectations of potential improved treatment outcomes.
• Adrenocortical hypoperfusion detected by contrast-enhanced ultrasound in a dog with trilostane-induced hypoadrenocorticism.
  N Nagata; K Aoshima; K Nakamura; M Takiguchi
  The Journal of small animal practice, 64, 11, 722, 726, Nov. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, A 12-year-old neutered male Chihuahua dog was diagnosed with pituitary-dependent hypercortisolism and treated with trilostane. Eighty-nine days later, the dog showed lethargy accompanied by hyponatraemia and hyperkalaemia. Hypoadrenocorticism due to trilostane was suspected, but the result of the adrenocorticotropic hormone stimulation test was not conclusive. Contrast-enhanced ultrasound showed loss of adrenocortical blood flow in both adrenal glands, indicating adrenocortical hypoperfusion and isolated hypoadrenocorticism. Treatment with fludrocortisone acetate improved the condition and electrolyte abnormalities. Thirteen months later, the dog showed alopecia, and an adrenocorticotropic hormone stimulation test revealed increased cortisol concentration, indicating hypercortisolism recurrence. The dog died due to progressive deterioration 22 months after the initial presentation. Post-mortem examination revealed focally extensive necrosis with marked calcification in the parenchyma of the adrenal glands and regeneration of the cells in the zona fasciculata with severe fibrosis. Adrenocortical hypoperfusion detected by contrast-enhanced ultrasound can support the diagnosis of adrenal necrosis and hypoadrenocorticism.
• The role of heat shock protein 90 in the proliferation of Babesia gibsoni in vitro.
  Hiroka Tsutsumi; Moeko Abe; Naohiro Uchida; Mitsuyoshi Takiguchi; Masahiro Yamasaki
  Experimental parasitology, 251, 108567, 108567, Aug. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The present study investigated the role of heat shock protein 90 (HSP90) in the proliferation and survival of Babesia gibsoni in vitro. To detect the effect on the entry of B. gibsoni into host erythrocytes, the parasite was incubated with an antibody against B. gibsoni HSP90 (BgHSP90) for 24 h. The results of this experiment demonstrated that both the incorporation of [3H]hypoxanthine into the nucleic acids of B. gibsoni and the number of parasites were not altered, indicating that an anti-BgHSP90 antibody did not directly inhibit the entry of the parasite into erythrocytes. Moreover, two HSP90 inhibitors, geldanamycin (GA) and tanespimycin (17-AAG), were used to evaluate the function of BgHSP90. GA and 17-AAG decreased both the incorporation of [3H]hypoxanthine and the number of infected erythrocytes, suggesting that BgHSP90 plays important roles in DNA synthesis and the proliferation of B. gibsoni. The effect of 17-AAG on the parasites was weaker than that of GA. Additionally, the effect of GA on the survival and superoxide generation of canine neutrophils was assessed. The survival of canine neutrophils was not affected. The superoxide generation was strongly suppressed by GA. This result indicated that GA inhibited the function of canine neutrophils. Additional studies are necessary to elucidate the role of BgHSP90 in the proliferation of the parasite.
• Diverse genome-wide DNA methylation alterations in canine hepatocellular tumours.
  Yu Asari; Jumpei Yamazaki; Oo Thandar; Tamami Suzuki; Keisuke Aoshima; Kyosuke Takeuchi; Ryohei Kinoshita; Sangho Kim; Kenji Hosoya; Teita Ishizaki; Yumiko Kagawa; Jaroslav Jelinek; Shoko Yokoyama; Noboru Sasaki; Hiroshi Ohta; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Veterinary medicine and science, 22 Jul. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Canine hepatocellular tumours (HCTs) are common primary liver tumours. However, the exact mechanisms of tumourigenesis remain unclear. Although some genetic mutations have been reported, DNA methylation alterations in canine HCT have not been well studied. OBJECTIVES: In this study, we aimed to analyse the DNA methylation status of canine HCT. METHODS: Tissues from 33 hepatocellular carcinomas, 3 hepatocellular adenomas, 1 nodular hyperplasia, 21 non-tumour livers from the patients and normal livers from 5 healthy dogs were used. We analysed the DNA methylation levels of 72,367 cytosine-guanine dinucleotides (CpG sites) in all 63 samples. RESULTS AND CONCLUSIONS: Although a large fraction of CpG sites that were highly methylated in the normal liver became hypomethylated in tumours from most patients, we also found some patients with less remarkable change or no change in DNA methylation. Hierarchical clustering analysis revealed that 32 of 37 tumour samples differed from normal livers, although the remaining 5 tumour livers fell into the same cluster as normal livers. In addition, the number of hypermethylated genes in tumour livers varied among tumour cases, suggesting various DNA methylation patterns in different tumour groups. However, patient and clinical parameters, such as age, were not associated with DNA methylation status. In conclusion, we found that HCTs undergo aberrant and diverse patterns of genome-wide DNA methylation compared with normal liver tissue, suggesting a complex epigenetic mechanism in canine HCT.
• An inflammatory bowel disease-associated SNP increases local thyroglobulin expression to develop inflammation in miniature dachshunds
  Yong Bin Teoh; Jing-Jing Jiang; Takeshi Yamasaki; Noriyuki Nagata; Toshiki Sugawara; Rie Hasebe; Hiroshi Ohta; Noboru Sasaki; Nozomu Yokoyama; Kensuke Nakamura; Yumiko Kagawa; Mitsuyoshi Takiguchi; Masaaki Murakami
  Frontiers in Veterinary Science, 10, Frontiers Media SA, 14 Jul. 2023, [Peer-reviewed], [Corresponding author]
  English, Scientific journal, Inflammatory colorectal polyp (ICRP) in miniature dachshunds (MDs) is a chronic inflammatory bowel disease (IBD) characterized by granulomatous inflammation that consists of neutrophil infiltration and goblet cell hyperplasia in the colon. Recently, we identified five MD-associated single-nucleotide polymorphisms (SNPs), namely PLG, TCOF1, TG, COL9A2, and COL4A4, by whole-exome sequencing. Here, we investigated whether TG c.4567C&gt;T (p.R1523W) is associated with the ICRP pathology. We found that the frequency of the T/T SNP risk allele was significantly increased in MDs with ICRP. In vitro experiments showed that TG expression in non-immune cells was increased by inducing the IL-6 amplifier with IL-6 and TNF-α. On the other hand, a deficiency of TG suppressed the IL-6 amplifier. Moreover, recombinant TG treatment enhanced the activation of the IL-6 amplifier, suggesting that TG is both a positive regulator and a target of the IL-6 amplifier. We also found that TG expression together with two NF-κB targets, IL6 and CCL2, was increased in colon samples isolated from MDs with the T/T risk allele compared to those with the C/C non-risk allele, but serum TG was not increased. Cumulatively, these results suggest that the T/T SNP is an expression quantitative trait locus (eQTL) of TG mRNA in the colon, and local TG expression triggered by this SNP increases the risk of ICRP in MDs via the IL-6 amplifier. Therefore, TG c.4567C&gt;T is a diagnostic target for ICRP in MDs, and TG-mediated IL-6 amplifier activation in the colon is a possible therapeutic target for ICRP.
• Successful treatment of sclerosing encapsulating peritonitis in a cat using bioresorbable hyaluronate-carboxymethylcellulose membrane after surgical adhesiolysis and long-term prednisolone
  Nozomu Yokoyama; Ryohei Kinoshita; Hiroshi Ohta; Kazuki Okada; Genya Shimbo; Kazuyoshi Sasaoka; Noriyuki Nagata; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Yumiko Kagawa; Mitsuyoshi Takiguchi
  Journal of Feline Medicine and Surgery Open Reports, 9, 2, SAGE Publications, Jul. 2023, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal, Case summary
  
  An 8-year-old neutered male domestic shorthair indoor cat was presented with an 8-week history of intermittent vomiting, anorexia and weight loss that had been unresponsive to supportive treatment. Abdominal ultrasound revealed plication of the small intestine and fluid accumulation proximal to the lesion, and a linear foreign body was suspected. An exploratory celiotomy showed cocoon-like encapsulation of the entire intestine. Surgical adhesiolysis and full-thickness biopsy were performed, and histopathologic examination revealed mild thickening of the visceral peritoneum with fibrin deposition, as well as mild neutrophil and lymphocyte infiltration. These findings were compatible with sclerosing encapsulating peritonitis (SEP). The cat recovered well postoperatively and was discharged the next day. Prednisolone was administered for 7 weeks to prevent recurrence of SEP. Five months after surgery, the cat was re-presented with anorexia and chronic vomiting. Based on the clinical examination findings, recurrent SEP was suspected. At the second surgery, surgical adhesiolysis was repeated and a bioresorbable hyaluronate-carboxymethylcellulose membrane was used to cover the serosal surface and thus prevent adhesion formation. Histopathologic findings of the peritoneal biopsy specimen confirmed SEP. Long-term prednisolone treatment (1 mg/kg for the first dose and 0.5 mg/kg every 48 h for maintenance) was administered postoperatively. The cat survived for more than 1239 days without recurrence.
  
  Relevance and novel information
  
  To our knowledge, this is the first report of SEP in a cat with long-term survival. The use of a bioresorbable hyaluronate-carboxymethylcellulose membrane and long-term prednisolone treatment may have prevented short-term and long-term recurrence, respectively, in this case.
• Investigation of the therapeutic effects, predictors, and complications of long-term immunosuppressive therapy in dogs with precursor-targeted immune-mediated anemia.
  Mei Sugawara-Suda; Keitaro Morishita; Yuto Iwanaga; Jumpei Yamazaki; Yumiko Kagawa; Nozomu Yokoyama; Noboru Sasaki; Hiroshi Ohta; Kensuke Nakamura; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 85, 7, 695, 701, 01 Jul. 2023, [Peer-reviewed], [Last author], [Domestic magazines]
  English, Scientific journal, Dogs with precursor-targeted immune-mediated anemia (PIMA) are commonly treated with immunosuppressive therapy, but information on predictors of treatment response and response time is limited. Therefore, we retrospectively investigated predictive factors that influenced the treatment response and duration required to observe a response in dogs with PIMA receiving continuous immunosuppressive therapies for more than 105 days. Of 50 client-owned dogs that developed PIMA, 27 were included in this study, of which 18 were responders and 9 were non-responders to immunosuppressive therapies. Sixteen of the 18 responders responded to treatment within 60 days and the remaining 2 responded at 93 and 126 days, respectively. We found that an erythroid-maturation ratio of <0.17 may be a useful predictor for treatment response. In addition, complications of immunosuppressive therapies were investigated further in 50 dogs. Pancreatitis (n=4) and pneumonia (3) occurred over the entire treatment period, and infections such as abscesses (3) tended to be more common in dogs on an extended period of immunosuppressive therapy. These findings may be helpful when planning for the initial treatment and may provide evidence for informed consent about potential comorbidities throughout the treatment course.
• Effectiveness of 2‐dimensional shear wave elastography for noninvasive and reliable estimation of right atrial pressure in dogs with induced volume overload
  Masahiro Tamura; Hiroshi Ohta; Tatsuyuki Osuga; Mitsuyoshi Takiguchi
  Journal of Veterinary Internal Medicine, Wiley, 10 Apr. 2023, [Peer-reviewed], [Corresponding author]
  Scientific journal
• Evaluation of responses to immunosuppressive therapy in dogs with suspected non‐regenerative immune‐mediated anaemia: 11 cases (2012‐2018)
  K. Morishita; M. Sugawara‐Suda; J. Yamazaki; N. Sasaki; K. Nakamura; H. Ohta; M. Takiguchi
  Journal of Small Animal Practice, 64, 527, Wiley, 07 Apr. 2023, [Peer-reviewed], [Last author]
  English, Scientific journal
• Zoobiquity experiments show the importance of the local MMP9-plasminogen axis in inflammatory bowel diseases in both dogs and patients.
  Takeshi Yamasaki; Noriyuki Nagata; Toru Atsumi; Rie Hasebe; Yuki Tanaka; Izuru Ohki; Shimpei Kubota; Yuta Shinohara; Yong Bin Teoh; Nozomu Yokoyama; Noboru Sasaki; Kensuke Nakamura; Hiroshi Ohta; Takehiko Katsurada; Yoshihiro Matsuno; Shintaro Hojyo; Shigeru Hashimoto; Mitsuyoshi Takiguchi; Masaaki Murakami
  International immunology, 35, 7, 313, 326, 18 Mar. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Using a zoobiquity concept, we directly connect animal phenotypes to a human disease mechanism: the reduction of local plasminogen levels caused by matrix metalloproteinase-9 (MMP9) activity is associated with the development of inflammation in the intestines of dogs and patients with inflammatory bowel disease. We first investigated inflammatory colorectal polyps (ICRPs), which are a canine gastrointestinal disease characterized by the presence of idiopathic chronic inflammation, in Miniature Dachshund (MD), and found 31 missense disease-associated SNPs by whole-exome sequencing. We sequenced them in 10 other dog breeds and found five, PLG, TCOF1, TG, COL9A2, and COL4A4, only in MD. We then investigated two rare and breed-specific missense SNPs (T/T SNPs), PLG: c.477G>T and c.478A>T, and found that ICRPs with the T/T SNP risk-alleles showed less intact plasminogen and plasmin activity in the lesions compared to ICRPs without the risk-alleles but no differences in serum. Moreover, we show that MMP9, which is a NF-κB target, caused the plasminogen reduction and that intestinal epithelial cells expressing plasminogen molecules were colocalized with epithelial cells expressing MMP9 in normal colons with the risk-alleles. Importantly, MMP9 expression in patients with ulcerous colitis or Crohn's disease also colocalized with epithelial cells showing enhanced NF-κB activation and less plasminogen expression. Overall, our zoobiquity experiments showed that MMP9 induces the plasminogen reduction in intestine, contributing to the development of local inflammation and suggesting the local MMP9-plasminogen axis is a therapeutic target in both dogs and patients. Therefore, zoobiquity-type experiments could bring new perspectives for biomarkers and therapeutic targets.
• Prognostic value of left atrial stiffness estimated using echocardiography in dogs with myxomatous mitral valve disease
  T. Osuga; K. Kuroda; T. Morita; N. Sasaki; K. Nakamura; M. Takiguchi
  Journal of Veterinary Cardiology, 45, 15, 26, Feb. 2023, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal
• Transcriptome and proteome analysis of dogs with precursor targeted immune-mediated anemia treated with splenectomy.
  Mei Sugawara-Suda; Keitaro Morishita; Osamu Ichii; Takashi Namba; Keisuke Aoshima; Yumiko Kagawa; Sangho Kim; Kenji Hosoya; Nozomu Yokoyama; Noboru Sasaki; Kensuke Nakamura; Jumpei Yamazaki; Mitsuyoshi Takiguchi
  PloS one, 18, 5, e0285415, 2023, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, Precursor-targeted immune-mediated anemia (PIMA) in dogs is characterized by persistent non-regenerative anemia and ineffective erythropoiesis, and it is suspected to be an immune-mediated disease. Most affected dogs respond to immunosuppressive therapies; however, some are resistant. In this study, we carried out splenectomy as an alternative therapy for refractory PIMA in dogs, and analyzed gene expression levels in the spleen of dogs with or without PIMA and in serum before and after splenectomy. A total of 1,385 genes were found to express differentially in the spleens from dogs with PIMA compared with healthy dogs by transcriptome analysis, of which 707 genes were up-regulated, including S100A12, S100A8, and S100A9 that are linked directly to the innate immune system and have been characterized as endogenous damage-associated molecular patterns. Furthermore, immunohistochemistry confirmed that S100A8/A9 protein expression levels were significantly higher in dogs with PIMA compared with those in healthy dogs. A total of 22 proteins were found to express differentially between the serum samples collected before and after splenectomy by proteome analysis, of which 12 proteins were up-regulated in the samples before. The lectin pathway of complement activation was identified by pathway analysis in pre-splenectomy samples. We speculated that S100A8/9 expression may be increased in the spleen of dogs with PIMA, resulting in activation of the lectin pathway before splenectomy. These findings further our understanding of the pathology and mechanisms of splenectomy for PIMA.
• Biological effects related to exposure to polychlorinated biphenyl (PCB) and decabromodiphenyl ether (BDE-209) on cats
  Khidkhan K; Mizukawa H; Ikenaka Y; Nakayama SMM; Nomiyama K; Yokoyama N; Ichii O; Takiguchi M; Tanabe S; Ishizuka M
  PLOS ONE, 18, 1, e0277689, Jan. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, As an animal familiar to humans, cats are considered to be sensitive to chemicals; cats may be exposed to polychlorinated biphenyls (PCBs) and decabromodiphenyl ether (BDE-209) from indoor dust, household products, and common pet food, leading to adverse endocrine effects, such as thyroid hormone dysfunction. To elucidate the general biological effects resulting from exposure of cats to PCBs and PBDEs, cats were treated with a single i.p. dose of a principal mixture of 12 PCBs and observed for a short-term period. Results revealed that the testis weight, serum albumin, and total protein of the treated group decrease statistically in comparison with those in the control group. The negative correlations suggested that the decrease in the total protein and albumin levels may be disturbed by 4'OH-CB18, 3'OH-CB28 and 3OH-CB101. Meanwhile, the serum albumin level and relative brain weight decreased significantly for cats subjected to 1-year continuous oral administration of BDE-209 in comparison to those of control cats. In addition, the subcutaneous fat as well as serum high-density lipoprotein (HDL) and triglycerides (TG) levels increased in cats treated with BDE-209 and down-regulation of stearoyl-CoA desaturase mRNA expression in the liver occurred. These results suggested that chronic BDE-209 treatment may restrain lipolysis in the liver, which is associated with lipogenesis in the subcutaneous fat. Evidence of liver and kidney cell damage was not observed as there was no significant difference in the liver enzymes, blood urea nitrogen and creatinine levels between the two groups of both experiments. To the best of our knowledge, this is the first study that provides information on the biochemical effects of organohalogen compounds in cats. Further investigations on risk assessment and other potential health effects of PCBs and PBDEs on the reproductive system, brain, and lipid metabolism in cats are required.
• An experimental challenge model for Leishmania donovani in beagle dogs, showing a similar pattern of parasite burden in the peripheral blood and liver.
  Hiroya Konno; Nozomu Yokoyama; Yu Tamura; Keisuke Aoshima; Ryo Nakao; Mitsuyoshi Takiguchi; Ken Katakura
  Parasitology research, 121, 12, 3569, 3579, Dec. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Leishmania donovani and Leishmania infantum are closely related species. However, the former is considered the causative agent for anthroponotic visceral leishmaniasis (AVL), while the latter is known to be responsible for zoonotic visceral leishmaniasis (ZVL) with dogs as the main reservoir host. Although molecular detection of L. donovani from naturally infected dogs has been reported in AVL endemic areas, the experimental infection of dogs with this species is very limited. Here, we constructed an experimental canine visceral leishmaniasis (CVL) model with L. donovani infection using beagle dogs. During an observation period of 8 months after parasite inoculation, few clinical symptoms were observed in the three inoculated dogs. The overall hematological and biochemical data of the dogs showed normal levels, and there were no remarkable changes in the peripheral CD4+, CD8+, CD25+, or FoxP3+ T cell populations. Liver biopsy sampling was conducted to monitor the parasite burden in the liver. A similar pattern of the amount of mitochondrial kinetoplast DNA was observed in the peripheral blood and liver by real-time PCR analysis. In addition, parasite antigens were detected from the liver biopsy sections by immunohistochemical analysis, further supporting the existence of parasites in the liver. These results showed a subclinical CVL model for L. donovani in beagle dogs with a similar kinetics of parasite burden in the peripheral blood and liver.
• Health impact assessment of pet cats caused by organohalogen contaminants by serum metabolomics and thyroid hormone analysis.
  Kei Nomiyama; Yasuo Yamamoto; Akifumi Eguchi; Hiroyuki Nishikawa; Hazuki Mizukawa; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Shouta M M Nakayama; Yoshinori Ikenaka; Mayumi Ishizuka
  The Science of the total environment, 842, 156490, 156490, 10 Oct. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Companion animals are in close contact with the human surroundings, and there is growing concern about the effects of harmful substances on the health of pet cats. In this study, we investigated the potential health effects of organohalogen compounds (OHCs) on thyroid hormone (TH) homeostasis and metabolomics in Japanese pet cats. There was a significant negative correlation between concentrations of several contaminants, such as polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), hydroxylated PCBs (OH-PCBs), hydroxylated PBDEs (OH-PBDEs), and THs in cat serum samples. These results suggested that exposure to OHCs causes a decrease in serum TH levels in pet cats. In this metabolomics study, each exposure level of parent compounds (PCBs and PBDEs) and their hydroxylated compounds (OH-PCBs and OH-PBDEs) were associated with their own unique primary metabolic pathways, suggesting that parent and phenolic compounds exhibit different mechanisms of action and biological effects. PCBs were associated with many metabolic pathways, including glutathione and purine metabolism, and the effects were replicated in in-vivo cat PCB administration studies. These results demonstrated that OHC exposure causes chronic oxidative stress in pet cats. PBDEs were positively associated with alanine, aspartate, and glutamate metabolism. Due to the chronic exposure of cats to mixtures of these contaminants, the combination of their respective metabolic pathways may have a synergistic effect.
• Local cerebral blood flow assessment using transcranial Doppler ultrasonography in a dog with brain infarction in the right middle cerebral artery territory.
  Kazuyoshi Sasaoka; Hiroshi Ohta; Tomohito Ishizuka; Tatsuyuki Osuga; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 84, 10, 1385, 1390, 01 Oct. 2022, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal, A 12-year-old neutered male Chihuahua was diagnosed with acute brain infarction in the right middle cerebral artery (MCA) territory. Transcranial Doppler ultrasonography (TCD) was performed to assess the local cerebral blood flow at the time of diagnosis and after 4 and 31 hr. Initially, the right MCA retained blood flow but with a lower cerebral blood flow velocity (CBFV; 14.9 cm/sec) than the left MCA (27.9 cm/sec). The TCD vascular resistance variables were higher in the right than in the left MCA. An increase in the CBFV and a decrease in TCD vascular resistance variables were observed, consistent with improvements in neurological symptoms. TCD can be a non-invasive, and easy-to-use modality for bedside monitoring of cerebral edema and infarction.
• Malignant oligoastrocytoma in the spinal cord of a cat.
  Dai Hasegawa; Keisuke Aoshima; Kazuyoshi Sasaoka; Atsushi Kobayashi; Mitsuyoshi Takiguchi; Takashi Kimura
  The Journal of veterinary medical science, 84, 9, 1277, 1282, Japanese Society of Veterinary Science, 12 Sep. 2022, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, A 12-year and 3-month spayed female mixed cat was presented with severe lumbar pain. Magnetic resonance imaging and postmortem examination revealed a swollen lesion in the spinal cord at L3 level. Histologic examination identified extensive neoplastic cell proliferation with massive necrosis in the tumor tissue. Two types of neoplastic cells were recognized. One type of neoplastic cells were large cells characterized by round to polygonal shape and abundant eosinophilic cytoplasm (referred to as "large cells"). The other neoplastic cells were small, densely proliferated, and had round to irregular shape and scant eosinophilic cytoplasm (referred to as "small cells"). Both types of cells were positive for oligodendrocyte transcription factor 2 and SRY-box transcription factor 10. Glial fibrillary acidic protein was positive in large cells but negative in most small cells. Digital analysis for Ki-67-stained tumor tissues found that total 21.1% ± 6.5% of tumor cells were positive for Ki-67. Based on these findings, we diagnosed malignant oligoastrocytoma in the spinal cord.
• Urinary corticoid to creatinine ratios using IMMULITE 2000 XPi for diagnosis of canine hypercortisolism.
  Noriyuki Nagata; Hiryu Sawamura; Keitaro Morishita; Kenji Hosoya; Nozomu Yokoyama; Kazuyoshi Sasaoka; Noboru Sasaki; Kensuke Nakamura; Yoshinori Ikenaka; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 84, 7, 954, 959, 10 Jul. 2022, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal, The urinary corticoid to creatinine ratio (UCCR) is one of the most commonly used screening tests for canine hypercortisolism (HC). In this study, a reference interval was established for UCCR using IMMULITE 2000 XPi, the latest chemiluminescence enzyme immunoassay. The diagnostic performance of this method for UCCR in canine HC was also evaluated. The median UCCR was 1.06 × 10-5 (range: 0.28-2.49) for 58 healthy dogs, and an upper reference limit of 1.98 × 10-5 (90% confidence interval: 1.76-2.15) was determined. The median UCCR in the 12 dogs with HC (7.38 × 10-5, range 1.86-29.98) was significantly higher than that in the 16 dogs with mimic-HC (1.59 × 10-5, range 0.47-3.42, P<0.001). The area under the curve for UCCR to differentiate HC dogs from mimic-HC dogs was 0.971, with a sensitivity of 91.7% and specificity of 100% when the cut-off value was set at 3.77 × 10-5. The UCCR of 16 paired urine samples collected at home and in hospital showed that the UCCR of samples collected in the hospital was significantly higher than that of samples collected at home (mean difference 3.30 × 10-5, 95% confidence interval: 0.70-5.90, P=0.001). In summary, we established the upper reference limit for UCCR using IMMULITE 2000 XPi in dogs and confirmed that UCCR is a useful diagnostic test for HC in dogs if urine samples are collected at home.
• Characterization of mucin gene expression and goblet cell proportion in inflammatory colorectal polyps in miniature dachshunds.
  Noriyuki Nagata; Hiroshi Ohta; Nozomu Yokoyama; Yong Bin Teoh; Noboru Sasaki; Kensuke Nakamura; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 84, 6, 872, 876, 18 Jun. 2022, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal, Hyperplastic goblet cells and abundant mucus are significant characteristics of inflammatory colorectal polyps (ICRPs) in miniature dachshunds. In this study, selected mucin gene expressions and goblet cell proportions were evaluated in miniature dachshunds with ICRPs and in healthy dogs. Mucin 2 (MUC2) gene expression was not significantly different among the groups, whereas mucin 5AC (MUC5AC) gene expression was significantly higher in the polypoid lesions than in healthy colonic mucosa. Although the percentage of goblet cells in the upper crypt regions did not significantly differ between the groups, that in the lower crypt regions was significantly decreased in polypoid lesions. In conclusion, increased MUC5AC gene expression and goblet cell proportion changes may be associated with the pathogenesis of ICRPs.
• Incremental predictive value of echocardiographic indices of right ventricular function in the assessment of long-term prognosis in dogs with myxomatous mitral valve disease
  T. Morita; K. Nakamura; T. Osuga; M. Takiguchi
  Journal of Veterinary Cardiology, 39, 51, 62, Feb. 2022, [Peer-reviewed], [Last author]
  Scientific journal
• Transcranial Doppler ultrasonography detects the elevation of cerebral blood flow during ictal-phase of pentetrazol-induced seizures in dogs.
  Kazuyoshi Sasaoka; Hiroshi Ohta; Tomohito Ishizuka; Kazuki Kojima; Noboru Sasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 83, 4, 331, 338, 28 Jan. 2022, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVE: To investigate the association between changes in cerebral blood flow and electrographic epileptic seizure in dogs using transcranial Doppler ultrasonography (TCD). ANIMALS: 6 healthy Beagle dogs. PROCEDURES: Each dog was administered pentetrazol (1.5 mg/kg/min) or saline (0.9% NaCl) solution under general anesthesia with continuous infusion of propofol. Both pentetrazol and saline solution were administered to all 6 dogs, with at least 28 days interval between the experiments. Blood flow waveforms in the middle cerebral artery and the basilar artery were obtained using TCD at baseline, after pentetrazol administration, and after diazepam administration. TCD velocities, including peak systolic velocity, end-diastolic velocity, and mean velocity and resistance variables, were determined from the Doppler waveforms. RESULTS: During ictal-phase of pentetrazol-induced seizures, the TCD velocities significantly increased in the basilar and middle cerebral arteries while TCD vascular resistance variables did not change in either artery. The TCD velocities significantly decreased after diazepam administration. Systemic parameters, such as the heart rate, mean arterial pressure, systemic vascular resistance, cardiac index, end-tidal carbon dioxide, oxygen saturation, and body temperature, did not change significantly during seizures. CLINICAL RELEVANCE: This study showed that cerebral blood flow, as obtained from TCD velocities, increased by 130% during ictal-phase of pentetrazol-induced seizures in dogs. The elevated velocities returned to baseline after seizure suppression. Thus, TCD may be used to detect electrographic seizures during the treatment of status epilepticus in dogs, and further clinical studies clarifying the association between changes in cerebral blood flow and non-convulsive seizure cases are needed.
• Serum steroid profiling of hepatocellular carcinoma associated with hyperadrenocorticism in dogs: A preliminary study.
  Thandar Oo; Noboru Sasaki; Yoshinori Ikenaka; Takahiro Ichise; Noriyuki Nagata; Nozomu Yokoyama; Kazuyoshi Sasaoka; Keitaro Morishita; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Frontiers in veterinary science, 9, 1014792, 1014792, 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common primary liver tumors in humans and dogs. Excessive adrenocortical hormone exposure may cause steroid hepatopathy, which may develop into HCC. In our previous study, hyperadrenocorticism (HAC) was a highly concurrent disease in dogs with HCC. Therefore, this study hypothesized that adrenal steroid alterations might be involved in hepatocarcinogenesis and aimed to specify the relationship between HAC and HCC in dogs. MATERIALS AND METHODS: This study included 46 dogs brought to the Hokkaido University Veterinary Teaching Hospital between March 2019 and December 2020. Owners gave their signed consent for blood collection on their first visit. A total of 19 steroids (14 steroids and 5 metabolites) in the baseline serum of 15 dogs with HCC, 15 dogs with HAC, and 10 dogs with both diseases were quantitatively measured using the developed liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method. RESULTS: In each group, 11 steroids were detected higher than 50%. The detection rate of steroid hormones did not significantly differ between the groups (p > 0.05). Principle component analysis (PCA) showed that the steroid profiles of the three groups were comparable. Median steroid hormone concentrations were not significantly different between the study diseases (p > 0.05). CONCLUSION: The developed LC/MS/MS was useful for measuring steroid hormones. Although it was clear that HAC was concurrent in dogs with HCC, none of the serum steroids was suggested to be involved in HCC.
• Evaluation of Right Ventricular Function and Dyssynchrony in a Dog Model of Acute Pulmonary Embolism: Diagnostic Utility and Reversibility.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Sei Kawamoto; Shingo Miki; Mitsuyoshi Takiguchi
  Frontiers in veterinary science, 9, 861064, 861064, 2022, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, Background: The diagnosis of acute pulmonary thromboembolism is challenging in dogs. Little has been reported on changes in echocardiographic indices in dogs with acute pulmonary thromboembolism. The objective of this study was to validate the relationship between echocardiographic indices and right heart catheterization variables in dogs with acute pulmonary embolism and to identify a useful echocardiographic index for diagnosing acute pulmonary embolism. Materials and Methods: Six healthy laboratory beagles were included in the study. Echocardiography and right heart catheterization were performed in a dog model of acute pulmonary embolism produced by microsphere injection. Echocardiographic indices, including the right ventricular (RV) Tei index, RV longitudinal strain, and the dyssynchrony index using speckle tracking echocardiography, transmitral flow, and eccentricity index, were measured. Results: The mean pulmonary arterial pressure increased (22.2 ± 1.2 mmHg) and the blood pressure decreased after microsphere injection. Although the mean pulmonary arterial pressure remained elevated, the blood pressure recovered 2 days after the microsphere injection. Most echocardiographic indices of RV function were significantly impaired following microsphere injection and recovered after 2 days. In contrast, the RV Tei index was significantly impaired after microsphere injection and the impairment persisted after 2 days. Multivariable analysis revealed that the RV Tei index was an independent echocardiographic predictor of pulmonary vascular resistance (β = 0.88, P < 0.001), and transmitral early diastolic wave was an independent predictor of the cardiac index (β = -0.86, P = 0.001). Conclusions: The RV Tei index is a useful echocardiographic index for diagnosing acute pulmonary embolism. Ventricular interdependence may be an important factor causing low cardiac output in dogs with acute pulmonary embolism.
• Case Report: Usefulness of Drip Infusion Cholangiography With Computed Tomography for the Diagnosis of Biloma in a Dog.
  Masahiro Tamura; Hiroshi Ohta; Nene Hasegawa; Kenji Hosoya; Mitsuyoshi Takiguchi
  Frontiers in veterinary science, 9, 854042, 854042, 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Bilomas are encapsulated collections of bile outside or inside the biliary tract within the abdominal cavity. For diagnostic and therapeutic approaches, it is important to identify the origin of bile leaks from the biliary tract. This case report describes the usefulness of drip infusion cholangiography with computed tomography (DIC-CT) for detecting the site of bile leakage in a dog with biloma. A 10-year-old, castrated male Pomeranian dog was referred to our department for gastrointestinal signs. Abdominal ultrasonography detected gallbladder mucocele without evidence of defect on the wall and well-defined anechoic localized fluid accumulation around the right division of the liver. On the other hand, there was only a small amount of ascites in the abdominal cavity. The accumulated fluid collected through abdominocentesis had a bilirubin concentration of 11.4 mg/dl, which was more than twice as high as that in serum (0.4 mg/dl), but had absence of pyogenic bacteria. The DIC-CT with meglumine iotroxate showed two well-defined large fluid collections: one between right medial and lateral lobe and the other between the right lateral lobe and caudate process of caudate lobe. Three-dimensional DIC-CT views that the former was enhanced by the contrast agent and that it communicated with an intrahepatic bile duct of the right lateral lobe. Moreover, the DIC-CT images confirmed communication with each fluid collections. After 6 days of hospitalization, a decrease in the amount of accumulated fluid was confirmed, after which cholecystectomy was performed. The dog was discharged from the hospital without complications. No signs of bile leakage were observed on follow-up imaging on postoperative day 10. According to authors knowledge, this has been the first report to show that DIC-CT can be useful for determining the origin of bile leakage in dogs with bilomas.
• Safety Assessment of Ultrasound-Assisted Intravesical Chemotherapy in Normal Dogs: A Pilot Study.
  Noboru Sasaki; Yoshinori Ikenaka; Keisuke Aoshima; Teiichiro Aoyagi; Nobuki Kudo; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Frontiers in pharmacology, 13, 837754, 837754, 2022, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, Intravesical chemotherapy after transurethral resection is a treatment option in patients with non-muscle invasive bladder cancer. The efficacy of intravesical chemotherapy is determined by the cellular uptake of intravesical drugs. Therefore, drug delivery technologies in the urinary bladder are promising tools for enhancing the efficacy of intravesical chemotherapy. Ultrasound-triggered microbubble cavitation may enhance the permeability of the urothelium, and thus may have potential as a drug delivery technology in the urinary bladder. Meanwhile, the enhanced permeability may increase systemic absorption of intravesical drugs, which may increase the adverse effects of the drug. The aim of this preliminary safety study was to assess the systemic absorption of an intravesical drug that was delivered by ultrasound-triggered microbubble cavitation in the urinary bladder of normal dogs. Pirarubicin, a derivative of doxorubicin, and an ultrasound contrast agent (Sonazoid) microbubbles were administered in the urinary bladder. Ultrasound (transmitting frequency 5 MHz; pulse duration 0.44 μsec; pulse repetition frequency 7.7 kHz; peak negative pressure -1.2 MPa) was exposed to the bladder using a diagnostic ultrasound probe (PLT-704SBT). The combination of ultrasound and microbubbles did not increase the plasma concentration of intravesical pirarubicin. In addition, hematoxylin and eosin staining showed that the combination of ultrasound and microbubble did not cause observable damages to the urothelium. Tissue pirarubicin concentration in the sonicated region was higher than that of the non-sonicated region in two of three dogs. The results of this pilot study demonstrate the safety of the combination of intravesical pirarubicin and ultrasound-triggered microbubble cavitation, that is, ultrasound-assisted intravesical chemotherapy.
• Prognostic value of left ventricular-arterial coupling estimated using echocardiography in dogs with myxomatous mitral valve disease.
  Tatsuyuki Osuga; Tomoya Morita; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 35, 6, 2607, 2615, 06 Nov. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: The interaction between the left ventricle (LV) and systemic arterial systems, known as left ventricular-arterial coupling (VAC), has been evaluated based on the effective arterial elastance (Ea) to LV end-systolic elastance (Ees) ratio (Ea/Ees). The Ea reflects the total arterial load of LV, whereas Ees reflects the LV systolic function. A recent study found that inappropriate VAC based on increased Ea/Ees estimated by echocardiography is associated with advanced disease severity in dogs with myxomatous mitral valve disease (MMVD). HYPOTHESIS: Inappropriate VAC assessed by echocardiographic estimation of Ea/Ees is associated with a worse prognosis in dogs with MMVD. ANIMALS: Eighty-nine dogs with MMVD. METHODS: Prospective cohort study. Dogs underwent echocardiographic examinations at enrollment. The Ea was estimated using the formula: mean blood pressure/(forward stroke volume/body weight). The Ees was estimated using the formula: mean blood pressure/(LV end-systolic volume/body weight). The Ea/Ees was calculated. RESULTS: By end of study, 22 dogs died of cardiac-related causes with 67 dogs censored. Dogs with increased Ea/Ees (Ea/Ees >0.34; median survival time, 527 days; 95% confidence interval [CI], 322 days-not determinable) had a shorter survival time (P < .0001) than those without increased Ea/Ees (Ea/Ees ≤0.34; median survival time, >1112 days; 95% CI, not determinable). Multivariate Cox proportional hazard analysis showed that Ea/Ees, body weight, peak systolic mitral annular velocity, and the peak early diastolic transmitral velocity-to-peak early diastolic mitral annular velocity ratio were independent predictors of cardiac-related death among echocardiographic indices. CONCLUSIONS AND CLINICAL IMPORTANCE: Inappropriate VAC assessed based on echocardiographically-estimated Ea/Ees is associated with a worse prognosis in dogs with MMVD.
• The use of 4-dimensional magnetic resonance angiography as a noninvasive procedure to diagnose subclavian steal syndrome in a dog.
  Genya Shimbo; Ryosuke Echigo; Mitsuyoshi Takiguchi
  Journal of the American Veterinary Medical Association, 259, 9, 1040, 1042, 01 Nov. 2021, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, CASE DESCRIPTION: A 5-year-old 4.1-kg (9.0-lb) spayed female Toy Poodle was referred because of a 6-month history of sporadic signs of neck pain. CLINICAL FINDINGS: Diagnostic imaging with MRI and CT revealed a dilated radicular artery connecting the right and left vertebral arteries and causing mild compression of the spinal cord. The left subclavian artery caudal to the origin of the left vertebral artery was absent. Subclavian steal syndrome (SSS) was suspected. TREATMENT AND OUTCOME: The owner declined surgical treatment; thus, the dog was treated conservatively with glucocorticoids and analgesics. Eight months later, the dog's clinical signs were unchanged but palliated with the administration of glucocorticoids and analgesics, and 4-dimensional (4-D) magnetic resonance angiography (MRA) revealed that the left vertebral artery received blood supply from the right vertebral artery through the dilated radicular artery and that the left vertebral artery caudal to this site had retrograde flow and drained into the left subclavian artery, confirming the diagnosis of SSS. The owner again declined surgery, and conservative treatment continued. The dog's condition was unchanged at the last follow-up communication 11 months after 4-D MRA. CLINICAL RELEVANCE: Subclavian steal syndrome is an extremely rare condition in dogs, and our findings suggested that 4-D MRA could be used to definitively diagnose SSS in dogs.
• Effects of intravenous administration of pimobendan on hemodynamic indices and indices of left atrial longitudinal strain derived from speckle-tracking echocardiography in healthy dogs.
  Shingo Miki; Kensuke Nakamura; Tomoya Morita; Tatsuyuki Osuga; Sei Kawamoto; Noboru Sasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 82, 10, 795, 801, Oct. 2021, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, OBJECTIVE: To determine the effects of IV administration of pimobendan on hemodynamic indices and indices of left atrial (LA) longitudinal strain by speckle-tracking echocardiography in healthy dogs. ANIMALS: 6 healthy Beagles. PROCEDURES: After the dogs were anesthetized, the right heart was catheterized and 2-D conventional and speckle-tracking echocardiography were performed before and after IV administration of 0.15 mg of pimobendan/kg. Speckle-tracking echocardiography was performed to assess the 3 LA phasic functions through LA deformation (longitudinal strain and strain rate) and volumetric analyses. RESULTS: Pimobendan significantly increased stroke volume and cardiac output and decreased systemic vascular resistance. Pimobendan significantly improved left ventricular function assessed by the Tei index and LA booster pump function assessed by LA longitudinal strain and change in fractional volume during atrial systole. Indices of LA reservoir and conduit function were unchanged. CONCLUSIONS AND CLINICAL RELEVANCE: In healthy dogs, IV administration of pimobendan improved the indices of LA booster pump function but had no effect on the indices of LA reservoir and conduit functions. Further studies are needed to validate whether these results can be extrapolated to dogs with myxomatous mitral valve disease.
• Genome-wide DNA methylation analysis of dogs with high lead exposure living near a lead mining area in Kabwe, Zambia.
  Jumpei Yamazaki; Haruya Toyomaki; Shouta M M Nakayama; John Yabe; Kaampwe Muzandu; Jaroslav Jelinek; Shoko Yokoyama; Yoshinori Ikenaka; Mitsuyoshi Takiguchi; Mayumi Ishizuka
  Environmental pollution (Barking, Essex : 1987), 286, 117229, 117229, 01 Oct. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Lead (Pb) is a heavy metal that has been proven to be toxic to both animals and humans. Genom-wide DNA methylation in domestic dogs exposed to high levels of Pb in Kabwe, Zambia was analyzed in this study. Using next-generation sequencing on samples from 20 domestic dogs (mean blood Pb concentration: 43.6 μg/dL and 7.2 μg/dL in the high and low exposure groups), a digital restriction enzyme analysis of methylation was performed to identify the genomic locations of differentially methylated CpG sites. A validation study on an additional 20 dogs followed (blood Pb concentration: 4.9-29.7 μg/dL). The cluster analysis resolved two broad clusters indicating high and low Pb exposure. The study identified 827 (1.2%) CpG sites with differences in methylation (101 CpG sites were hypermethylated in the low exposure group and 726 were hypermethylated in the high exposure group). The sites corresponded to 26 genes with differentially methylated CpG sites at their promoter regions, including the NGF gene. The methylation of four CpG sites was validated using bisulfite pyrosequencing. The results indicate that aberrant hypermethylation is prevalent in dogs exposed to Pb. The altered DNA methylation of the genes identified in this study contributes to a greater understanding of the epigenetic changes caused by Pb exposure and highlights novel biomarker discoveries across species.
• Obese status is associated with accelerated DNA methylation change in peripheral blood of senior dogs.
  Jumpei Yamazaki; Shinji Meagawa; Jaroslav Jelinek; Shoko Yokoyama; Noriyuki Nagata; Masashi Yuki; Mitsuyoshi Takiguchi
  Research in veterinary science, 139, 193, 199, Oct. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Obesity and its associated comorbidities constitute a major and growing health problem worldwide not only involved with people but also dogs and cats. Although few genetic mutations have been associated with obesity in dogs, molecular mechanism remains to be clearly understood. Given the fact that DNA methylation leads to gene expression variability and has plasticity affected by metabolic phenotypes such as obesity in human, the objective of this study is to identify obesity-associated differentially methylated cytosine-phosphate-guanine (CpG) dinucleotide sites in dogs. With genome-wide DNA methylation analysis using next-generation sequencing for blood samples from fourteen Miniature dachshunds with body condition score (BCS) 4-5 and BCS ≥6, over 100,000 sites could be analysed to identify genomic locations of differentially methylated CpG sites. As a result, 191 differentially methylated CpG sites (89 CpG sites were hypermethylated in BCS ≥6 and 102 were hypermethylated in BCS 4-5) were identified. These sites included promoter regions of Kisspeptin receptor (KISS1R) and Calcyphosine 2 (CAPS2) genes which were subsequently validated by bisulfite-pyrosequencing for another set of 157 dog blood samples. KISS1R methylation levels were found to be higher in BCS ≥6 group than BCS 4-5 in senior (>84 months) dogs. Especially male dogs but not female dogs as well as uncastrated male dogs but not castrated male dogs showed this trend. DNA methylation of KISS1R gene will be useful for understanding of comprehensive epigenetic change in obese dogs.
• Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method.
  Noboru Sasaki; Thandar Oo; Yoshikazu Yasuda; Takahiro Ichise; Noriyuki Nagata; Nozomu Yokoyama; Kazuyoshi Sasaoka; Keitaro Morishita; Shouta Mm Nakayama; Mayumi Ishizuka; Kensuke Nakamura; Mitsuyoshi Takiguchi; Yoshinori Ikenaka
  The Journal of veterinary medical science, 83, 11, 1634, 1642, 17 Sep. 2021, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2-16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.
• Genome-wide DNA methylation profile in feline haematological tumours: A preliminary study.
  Jumpei Yamazaki; Jaroslav Jelinek; Shoko Yokoyama; Mitsuyoshi Takiguchi
  Research in veterinary science, 140, 221, 228, 08 Sep. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Although DNA methylation has been analysed in few studies for a limited number of loci in cats with diseases, genome-wide profile of DNA methylation has never been addressed. The hypothesis for this study is that next-generation sequencing with sequential digestion of genomic DNA with SmaI and XmaI enzymes could provide highly quantitative information on methylation levels in cats. Using blood from four healthy control cats and two disease cats as well as three feline lymphoma/leukemia cell lines, approximately 74-94 thousand CpG sites across the cat genome could be analysed. CpG sites in CpG island (CGI) were broadly either methylated or unmethylated in normal blood, while CpG sites in non-CpG islands (NCGI) are largely methylated. Lymphoma cell lines showed thousands of CpG sites with gain of methylation at normally unmethylated CGI sites and loss of methylation at normally methylated NCGI sites. Hypermethylated CpG sites located at promoter regions included genes annotated with 'developmental process' and 'anatomical structure morphogenesis' such as HOXD10. This highly quantitative method would be suitable for studies of DNA methylation changes not only in cancer but also in other common diseases in cats.
• Systemic mucoid degeneration of the arterial tunica intima in a young dog.
  Yoshiki Ichikawa; Mizuki Heishima; Kristin Vyhnal; Keisuke Aoshima; Kazuyoshi Sasaoka; Ryohei Kinoshita; Atsushi Kobayashi; Mitsuyoshi Takiguchi; Takashi Kimura
  Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 34, 1, 10406387211042587, 10406387211042587, 04 Sep. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, A 27-mo-old, spayed female mixed-breed dog was presented with left forelimb pain, which progressed to full thickness necrosis of the soft tissues of multiple limbs. Clinical imaging and postmortem examination suggested multiple large arterial thromboemboli. Histologic examination of vascular lesions revealed markedly thickened tunica intima with polypoid intraluminal projections, which partially to entirely occluded the arterial lumen. The expanded tunica intima was comprised of intimal accumulation of Alcian blue-positive matrix with scattered spindle-to-satellite cells. These cells were positive for von Willebrand factor and vimentin but negative for α-smooth muscle actin, suggesting endothelial origin. Deposition of the intimal mucoid matrix was observed in the elastic and muscular arteries associated with regional ischemic changes. Mucoid emboli, likely from fragmentation of proliferative intimal tissue, were identified in smaller vessels supplied by affected arteries. Based on these findings, we diagnosed systemic mucoid degeneration of the arterial tunica intima. Such systemic arterial degeneration characterized by deposition of mucoid matrix in the tunica intima has not been reported previously in dogs, to our knowledge, and should be distinguished from thromboembolism and other degenerative vascular diseases.
• Cardiovascular effects of dose escalating of norepinephrine in healthy dogs anesthetized with isoflurane.
  Kazuki Kojima; Tomohito Ishizuka; Noboru Sasaki; Kensuke Nakamura; Mitsuyoshi Takiguchi
  Veterinary anaesthesia and analgesia, 48, 5, 654, 662, Sep. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To evaluate the systemic cardiovascular effects of dose escalating administration of norepinephrine in healthy dogs anesthetized with isoflurane. STUDY DESIGN: Experimental study. ANIMALS: A total of six adult laboratory Beagle dogs, 10.5 (9.2-12.0) kg [median (range)]. METHODS: Each dog was anesthetized with isoflurane at an end-tidal concentration of 1.7%, mechanically ventilated and administered a continuous rate infusion of rocuronium (0.5 mg kg-1 hour-1). Each dog was administered incremental dose rates of norepinephrine (0.05, 0.125, 0.25, 0.5, 1.0 and 2.0 μg kg-1 minute-1), and each dose was infused for 15 minutes. Cardiovascular variables were recorded before administration and at the end of each infusion period. RESULTS: Norepinephrine infusion increased mean arterial pressure (MAP), cardiac output (CO) and oxygen delivery in a dose-dependent manner. Systemic vascular resistance did not significantly change during the experiment. Stroke volume increased at the lower dose rates and heart rate increased at the higher dose rates. Oxygen consumption and lactate concentrations did not significantly change during infusions. CONCLUSIONS: In dogs anesthetized with isoflurane, norepinephrine increased MAP by increasing the CO. CO increased with a change in stroke volume at lower dose rates of norepinephrine. At higher dosage, heart rate also contributed to an increase in CO. Norepinephrine did not cause excessive vasoconstriction that interfered with the CO during this study. CLINICAL RELEVANCE: Norepinephrine can be useful for treating hypotension in dogs anesthetized with isoflurane.
• Peliosis Hepatis with Chylous Ascites in a Dog.
  Kevin Christian Montecillo Gulay; Noriyuki Nagata; Keisuke Aoshima; Nozomi Shiohara; Atsushi Kobayashi; Mitsuyoshi Takiguchi; Takashi Kimura
  Journal of comparative pathology, 187, 63, 67, Aug. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, A 6-year-old spayed female Toy Poodle dog was referred to the Hokkaido University Veterinary Teaching Hospital for abdominal distension. Abdominocentesis yielded ascitic fluid that had a mildly increased total protein concentration and a 2.7-fold higher triglyceride concentration than plasma, and was interpreted as chylous ascites. The patient had an enlarged liver, which contained multiple, small, nodular masses and cyst-like structures. Microscopically, these lesions were multifocal dilated spaces containing lymphocytes, endothelial cells, fibrin and islands of hepatocytes. Increased α-smooth muscle actin-positive cells were observed in hepatic sinusoids. Based on these findings, we diagnosed peliosis hepatis with chylous ascites, which is likely to have been due to lymphangiectasia and disrupted hepatic sinusoids. Neither Bartonella spp DNA nor mutations in ACVRL1 and MTM1 genes were detected, although there was a 47-fold increase in hepatic ACVRL1 expression compared with age-matched control liver. To the authors' knowledge, this is the first report of chylous ascites resulting from peliosis hepatis in any species.
• CT morphology of anomalous systemic arterial supply to normal lung in dogs.
  Genya Shimbo; Mitsuyoshi Takiguchi
  Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association, 62, 6, 657, 665, 11 Jul. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Anomalous systemic arterial supply to the normal lung (ASANL) is a rare congenital anomaly in humans, in which the systemic arteries supply the basal segments of the lower lobe. It has a normal bronchial connection, but lacks a normal pulmonary artery. This anomaly has not been previously reported in the veterinary literature. The objectives of this retrospective descriptive study were to characterize the CT findings and clinical features of ASANL , and to determine the breed predisposition in a population of referral canine cases. Thoracic CT images, in which the caudal lung lobes were fully inflated and the pulmonary artery could be traced to the periphery, were reviewed. A total of 1,950 dogs were enrolled, and the aberrant vasculature equivalent to ASANL in humans was detected in 48 dogs. Shetland Sheepdogs (7/48, odds ratio [OR] = 8.0, P < 0.00001), Miniature Dachshunds (19/48, OR = 3.9, P < 0.00001), and Labrador Retrievers (6/48, OR = 4.5, P = 0.0009) were over-represented. The affected lung lobes were the right caudal lobe (24/48, 50%), the left caudal lobe (21/48, 43.8%), and bilateral caudal lobes (3/48, 6.3%). The aberrant vessels originated from the left gastric artery (14/48), descending thoracic aorta (8/48), celiac artery (6/48), and splenic artery (1/48). In the remaining 19 cases, the origin of the aberrant vessels could not be determined. Although the clinical significance of ASANL in dogs remains unclear, surgeons should be aware of this finding prior to lobectomy of the caudal lung lobes to avoid intraoperative systemic arterial bleeding.
• Morphometric evaluation of canine hepatocellular carcinoma using computed tomography: a promising tool for predicting malignancy.
  Rommaneeya Leela-Arporn; Hiroshi Ohta; Genya Shimbo; Noboru Sasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 83, 9, 1459, 1464, 06 Jul. 2021, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, The size of canine focal liver lesions (FLLs) is known to be one of the predicting criteria for malignancy. However, there are discrepancies for the measurement of maximum lesion size, resulting in contradicting results among studies and incidences of false positive outcomes. Thus far, the morphometric changes of FLLs for distinguishing malignancy from benignancy remains undocumented. This study aimed to investigate morphometric characteristics of FLLs using computed tomography (CT). CT images of 40 dogs with histopathological confirmation of 49 liver lesions, including 39 hepatocellular carcinomas and 10 nodular hyperplasias were retrospectively reviewed. The morphometric parameters including size (long and short axis diameters measured on transverse image), shape (measured by long to short axis (L/S) ratio), volume, and surface appearance of a liver lesion were evaluated using univariate and stepwise multivariate analyses, respectively. The results of univariate analysis showed that long and short axis diameters, L/S ratio, volume, and surface appearance of a lesion were significantly different between hepatocellular carcinomas and nodular hyperplasias. Multivariate analysis revealed that short axis diameter (>3.30 cm; odds ratio (OR): 36.1, 95% confidence interval (CI): 3.36-387.05, P=0.0031) and L/S ratio (>1.23; OR: 18.1, 95% CI: 1.61-205.12, P=0.0191) were independent predictors of malignancy, with the area under the curve of 0.9154. These results suggest that the combination of short axis diameter and L/S ratio is a promising tool for predicting liver malignancy with outstanding discriminating ability.
• Extrahepatic biliary obstruction can interfere with hepatic fibrosis prediction using two-dimensional shear wave elastography in dogs.
  Masahiro Tamura; Hiroshi Ohta; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Mitsuyoshi Takiguchi
  Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association, 62, 4, 483, 489, Jul. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Two-dimensional shear wave elastography (2D-SWE) can be used to quantitatively evaluate the elastic modulus of the liver as shear wave velocity (SWV), which can noninvasively predict clinically relevant hepatic fibrosis in both dogs and humans. However, extrahepatic biliary obstruction (EHBO), regardless of the presence of clinically relevant hepatic fibrosis, can influence SWVs in humans and thus may interfere with hepatic fibrosis prediction using 2D-SWE in dogs. The aim of this prospective, observational, and one-group pretest-posttest study is to investigate whether SWV measured by 2D-SWE displays a difference between dogs with and without EHBO. A total of 20 dogs were included (7 with EHBO and 13 with gallbladder pathology but no EHBO) that underwent preoperative SWV measurement using 2D-SWE. In all dogs, stages of hepatic fibrosis were evaluated histopathologically using a scoring scheme. In addition, postoperative SWVs in dogs with EHBO relieved via laparotomy were also evaluated. The median (range) SWVs in the dogs with and without EHBO were 1.91 (1.81-2.54) m/s and 1.57 (1.37-1.64) m/s, respectively. Although there was no significant difference in the histopathological hepatic fibrosis stages between the dogs with and without EHBO, the preoperative SWVs in the dogs with EHBO were significantly higher than in dogs without EHBO (P = .0004), and SWVs were found to decrease significantly after surgery (P = .0097). This study demonstrates that EHBO can increase the SWV of dogs without clinically relevant hepatic fibrosis and can interfere with the prediction of noninvasive hepatic fibrosis using 2D-SWE.
• The effects of fipronil on emotional and cognitive behaviors in mammals.
  Tomohiro Suzuki; Anri Hirai; Kraisiri Khidkhan; Collins Nimako; Takahiro Ichise; Kazuki Takeda; Hazuki Mizukawa; Shouta M M Nakayama; Kei Nomiyama; Nobuhiko Hoshi; Mizuki Maeda; Tetsushi Hirano; Kazuyoshi Sasaoka; Noboru Sasaki; Mitsuyoshi Takiguchi; Mayumi Ishizuka; Yoshinori Ikenaka
  Pesticide biochemistry and physiology, 175, 104847, 104847, Jun. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Fipronil is a phenylpyrazole insecticide that is widely used as a pesticide and a veterinary drug, although studies suggest that it could be toxic to mammals. The objectives of this study were to examine the pharmacokinetic profile of fipronil in mice, dogs, and cats, and to evaluate its effects on emotional and cognitive behaviors of dogs and cats using the data obtained from mice. The assessment of in vivo kinetics of fipronil was conducted in mice and dogs. We also performed behavioral tests (elevated plus-maze and Y-maze) and measured the levels of neurotransmitters in mice exposed to fipronil. In addition, the in vitro metabolism of fipronil were evaluated using liver microsomes of rats, mice, dogs, and cats. The results revealed that fipronil is distributed throughout the body (blood, brain, adipose tissue, and liver) of mice after dermal application. It was metabolized to fipronil sulfone primarily in the liver. The data on kinetics show that both fipronil and fipronil sulfone have a longer half-life in dogs and cats than in mice. The behavioral tests indicated that fipronil and fipronil sulfone could affect emotional and cognitive behaviors and alter the levels of neurotransmitters (dopamine in the striatum and serotonin in the hippocampus) in mice. Furthermore, we found that dogs and cats have a low ability to metabolize fipronil than mice and rats. However, further comprehensive studies are needed to determine whether fipronil affects the emotional and cognitive behaviors when administered to dogs and cats. To the best of our knowledge, this is the first study to examine the pharmacokinetic data and verify the effects of fipronil on emotional and cognitive behaviors of dogs and cats using the data obtained from mice.
• DNA methylation landscape of 16 canine somatic tissues by methylation-sensitive restriction enzyme-based next generation sequencing.
  Jumpei Yamazaki; Yuki Matsumoto; Jaroslav Jelinek; Teita Ishizaki; Shingo Maeda; Kei Watanabe; Genki Ishihara; Junya Yamagishi; Mitsuyoshi Takiguchi
  Scientific reports, 11, 1, 10005, 10005, 11 May 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, DNA methylation plays important functions in gene expression regulation that is involved in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in companion animals like dog. Using methylation-sensitive restriction enzyme-based next generation sequencing (Canine DREAM), we obtained canine DNA methylation maps of 16 somatic tissues from two dogs. In total, we evaluated 130,861 CpG sites. The majority of CpG sites were either highly methylated (> 70%, 52.5-64.6% of all CpG sites analyzed) or unmethylated (< 30%, 22.5-28.0% of all CpG sites analyzed) which are methylation patterns similar to other species. The overall methylation status of CpG sites across the 32 methylomes were remarkably similar. However, the tissue types were clearly defined by principle component analysis and hierarchical clustering analysis with DNA methylome. We found 6416 CpG sites located closely at promoter region of genes and inverse correlation between DNA methylation and gene expression of these genes. Our study provides basic dataset for DNA methylation profiles in dogs.
• Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method.
  Noboru Sasaki; Yoshinori Ikenaka; Yumiko Inoue; Takahiro Ichise; Noriyuki Nagata; Mayumi Ishizuka; Syouta Mm Nakayama; Kensuke Nakamura; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 83, 4, 648, 655, 09 Apr. 2021, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, Measurement of urinary metanephrines in spot samples is used for the diagnosis of canine pheochromocytoma (PC). We describe a simple analytical method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for measuring free metanephrine (MN) and normetanephrine (NMN) in spot urine samples. Using the developed method, we evaluated the stability of urinary free-MN and free-NMN at various storing conditions. In addition, we assessed the feasibility of urinary free-MN and -NMN measurement for diagnosing PC. Urine samples were mixed with stable isotope internal standards and thereafter purified by ultrafiltration. The purified samples were analyzed by LC-MS/MS in the multiple reaction monitoring mode after separation on a multimode octa decyl silyl column. The coefficient of variation of free-MN and -NMN measurement was 7.6% and 5.5%, respectively. The linearity range was 0.5-10 µg/l for both analytes. Degradation was less than 10% for both analytes under any of the storage conditions. The median free-NMN ratio to creatinine of 9 PC dogs (595, range 144-47,961) was significantly higher (P<0.05) than that of 13 dogs with hypercortisolism (125, range 52-224) or 15 healthy dogs (85, range 50-117). The developed method is simple and may not require acidification of spot urine. The results of this preliminary retrospective study suggest that the measurement of urinary free metanephrines is a promising tool for diagnosing canine PC.
• Repeatability, reproducibility, and reference intervals for indices of right atrial longitudinal strain derived from speckle-tracking echocardiography in healthy dogs.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Mitsuyoshi Takiguchi
  American journal of veterinary research, 82, 4, 274, 279, Apr. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To determine repeatability, reproducibility, and reference intervals of indices of right atrial longitudinal strain (RALS) derived from speckle-tracking echocardiography (STE) in dogs without heart disease. ANIMALS: 110 client-owned dogs and 10 laboratory Beagles. PROCEDURES: To determine intraobserver within-day (repeatability) and interobserver (reproducibility) coefficients of variation, RALS during ventricular systole (ϵS), ventricular early diastole (ϵE), and atrial systole (ϵA), as derived with STE, were obtained by 2 investigators for 5 randomly selected client-owned dogs and analyzed by linear regression. Reference intervals were estimated from the results of all dogs. Correlations between RALS indices (ϵS, ϵE, and ϵA) and sex, age, body weight, heart rate, and blood pressure were determined. RESULTS: RALS derived from STE showed good intraobserver within-day repeatability and interobserver reproducibility, with coefficients of variation of < 20%. Both ϵS and ϵE were significantly negatively correlated with age, but ϵA was not correlated with age. Indices were not correlated with sex, body weight, or blood pressure. CONCLUSIONS AND CLINICAL RELEVANCE: RALS indices derived from STE were repeatable and reproducible and were affected by the age of dogs without heart disease. Age should be considered in the interpretation of RALS indices in clinical settings. Further studies are needed to apply RALS indices for assessing dogs with heart disease.
• Prognostic value of small intestinal dilatation in dogs with protein-losing enteropathy.
  Hiroshi Ohta; Noriyuki Nagata; Nozomu Yokoyama; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 83, 3, 378, 384, 11 Mar. 2021, [Peer-reviewed], [Last author], [Domestic magazines]
  English, Scientific journal, To date, little is known about the prognostic significance of ultrasonographic findings in dogs with protein-losing enteropathy (PLE). The aim of this retrospective study was to examine the prognostic value of ultrasonographic findings in dogs with PLE. A total of 26 dogs with PLE were included: 20 dogs with chronic enteropathy and 6 dogs with gastrointestinal lymphoma. The presence of small intestinal dilatation was associated with shorter survival time in dogs with PLE (P=0.003). The presence of hyperechoic intestinal mucosal striations was associated with longer survival time in dogs with PLE (P=0.0085). The results of the current study indicate that the presence of small intestinal dilatation might be associated with poor prognosis in dogs with PLE.
• Reduced expression levels of heat shock protein 90 in a diminazene aceturate-resistant Babesia gibsoni isolate.
  Masahiro Yamasaki; Natsuki Idaka; Moeko Abe; Mitsuyoshi Takiguchi
  Experimental parasitology, 221, 108050, 108050, Feb. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Heat shock protein 90 (HSP90) is a molecular chaperon and an essential component for stage differentiation and intracellular growth inside the host cells of many protozoans. HSP90 of Babesia gibsoni (BgHSP90) was suggested to function in the development of diminazene aceturate (DA)-resistance. Therefore, we examined the expression level of BgHSP90 in a DA-resistant B. gibsoni isolate. Transcription of the BgHSP90 gene in the DA-resistant isolate and wild-type B. gibsoni was assessed by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). As a result, the copy number and relative amount of BgHSP90 transcripts in the DA-resistant isolate were significantly lower than those in the wild-type. Moreover, a rabbit anti-recombinant BgHSP90 antibody was developed, and the protein synthesis of BgHSP90 in the DA-resistant isolate was compared with that in the wild-type by Western blot analysis and indirect fluorescence assay. There was significantly less BgHSP90 protein than in the wild-type. Additionally, the relative intensity of BgHSP70 in DA-resistant isolate was also lower than that in the wild-type. This suggested that the expression of BgHSP90 and BgHSP70 in the DA-resistant B. gibsoni isolate was suppressed and that the reduced amount of BgHSP90 and BgHSP70 might cause the weak proliferation of the DA-resistant isolate. Further studies are necessary to elucidate the function of BgHSP90.
• Echocardiographic estimation of left ventricular-arterial coupling in dogs with myxomatous mitral valve disease.
  Tatsuyuki Osuga; Tomoya Morita; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 35, 1, 78, 87, Jan. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: The effective arterial elastance (Ea) to left ventricular (LV) end-systolic elastance (Ees) ratio (Ea/Ees) is an index of the interaction between LV and systemic arterial systems, left ventricular-arterial coupling (VAC). The Ea is an index of total arterial load of the LV, whereas Ees is an index of LV systolic function. In humans, inappropriate VAC based on increased Ea/Ees estimated using echocardiography is associated with more advanced heart disease severity. HYPOTHESIS: Left ventricular-arterial coupling assessed by echocardiographic estimation of Ea/Ees is associated with disease severity in dogs with myxomatous mitral valve disease (MMVD). ANIMALS: Ninety MMVD dogs and 61 healthy dogs. METHODS: Prospective cross-sectional study. The MMVD dogs were classified into stages B1, B2, or C according to American College of Veterinary Internal Medicine guidelines. Effective arterial elastance was echocardiographically estimated using the formula: mean blood pressure/(forward stroke volume/body weight). End-systolic elastance was echocardiographically estimated using the formula: mean blood pressure/(LV end-systolic volume/body weight). The ratio Ea/Ees was calculated. RESULTS: The ratio Ea/Ees was higher in stage B2 dogs than in healthy dogs and dogs stage B1 (both P < .0001), and higher in stage C dogs than in healthy dogs and dogs in the other 2 stages (healthy vs C and B1 vs C, P < .0001; B2 vs C, P = .0005). Multivariable logistic regression analysis showed that Ea/Ees and the peak velocity of early diastolic transmitral flow to isovolumic relaxation time ratio were independent predictors of stage C among echocardiographic indices in MMVD dogs. CONCLUSIONS AND CLINICAL IMPORTANCE: Inappropriate VAC assessed by echocardiographically estimated Ea/Ees is associated with advanced disease severity in dogs with MMVD.
• Acute effects of intravenous pimobendan administration in dog models of chronic precapillary pulmonary hypertension
  T. Morita; K. Nakamura; T. Osuga; S. Kawamoto; S. Miki; K. Sasaoka; M. Takiguchi
  Journal of Veterinary Cardiology, 32, 16, 27, Dec. 2020, [Peer-reviewed], [Last author]
  Scientific journal
• Long interspersed nucleotide element-1 hypomethylation in canine malignant mucosal melanoma.
  Teita Ishizaki; Jumpei Yamazaki; Shinji Meagawa; Nozomu Yokoyama; Keisuke Aoshima; Mitsuyoshi Takiguchi; Takashi Kimura
  Veterinary and comparative oncology, 18, 4, 854, 860, Dec. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Canine malignant melanoma is a common cancer with a high mortality rate and is a clinically important disease. DNA methylation has been considered to be a potential tumorigenic mechanism through aberrant DNA methylation at promoter region which represses gene transcription. Global hypomethylation could also facilitate chromosome instability. There are few reports regarding DNA methylation in canine malignant melanoma; therefore, the purpose of this study was to examine DNA methylation status of long interspersed nucleotide element-1 (LINE-1) to be a surrogate marker of genome-wide methylation changes in this disease. We measured levels of DNA methylation of two adjacent cytosine-guanine sites on CpG island (CGI) at the putative promoter of canine LINE-1 sequence by bisulphite-pyrosequencing in 41 canine melanoma patient samples as well as six cell lines compared with normal mucosae. The survival rates were obtained from owners or medical records. We found DNA methylation levels of LINE-1 in normal mucosae were methylated. Interestingly, both melanoma cell lines and clinical melanoma samples showed remarkable hypomethylation. In addition, patients with lower LINE-1 methylation showed worse prognosis than those with higher LINE-1 methylation, though the difference did not reach statistical significance (P = .09). Here, we demonstrate that hypomethylation of LINE-1 is an epigenetically aberrant feature in canine melanoma with possible prognostic value.
• Wobbly hedgehog syndrome with disseminated histiocytic sarcoma and lateral ventricular meningioma in an African pygmy hedgehog.
  Lesa A Thompson; Atsuya Morita; Shoko Murakami; Noboru Sasaki; Miou Murashita; Ryou Yamazaki; Atsushi Kobayashi; Takashi Kimura; Mitsuyoshi Takiguchi
  Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 32, 6, 953, 956, Nov. 2020, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, An 8-mo-old male African pygmy hedgehog was anorectic and ataxic; physical examination revealed tetraparesis and a gangrenous left hindlimb. Analgesic and supportive care were administered, but the animal died 3 d after presentation. Postmortem examination revealed a histiocytic sarcoma in a mesenteric lymph node with metastasis to several organs, multifocal vacuolation in the cerebral and cerebellar white matter, and a meningioma in the left lateral ventricle. We diagnosed wobbly hedgehog syndrome (WHS) with disseminated histiocytic sarcoma and lateral ventricular meningioma. Ventricular meningioma, a rare neoplasm in veterinary and human patients, has not been reported previously in hedgehogs, to our knowledge. The neurologic signs in our case were probably caused by the WHS-related vacuolar lesions and are consistent with those of reported WHS cases. Duration of illness was shorter than is typical of WHS cases, which might be related to the disseminated histiocytic sarcoma. Clinical relevance of the lateral ventricular meningioma was not evident because the ventricular mass was localized and not invasive.
• Gene expression of leucine-rich alpha-2 glycoprotein in the polypoid lesion of inflammatory colorectal polyps in miniature dachshunds.
  Hiroshi Ohta; Yu Tamura; Nozomu Yokoyama; Noriyuki Nagata; Tatsuyuki Osuga; Noboru Sasaki; Yumiko Kagawa; Keitaro Morishita; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 82, 10, 1445, 1449, 20 Oct. 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Inflammatory colorectal polyps (ICRPs) in miniature dachshunds (MDs) are speculated to be a breed-specific inflammatory bowel disease (IBD). Leucine-rich alpha-2 glycoprotein (LRG) has been identified as a novel biomarker of human IBD. The aim of this study was to examine LRG gene expression in the polypoid lesions of ICRPs. Polypoid lesion specimens were collected from 24 MDs with ICRPs. Nonpolypoid colonic mucosa was collected from 18 MDs with ICRPs and 10 controls. The gene expression of LRG, interleukin (IL)-1β, IL-6, tumor necrosis factor-α, and IL-22 was examined. The expression of LRG gene was significantly increased in the polypoid lesions of ICRPs and correlated with that of the four cytokines. In conclusion, the LRG gene was expressed within the polypoid lesions of ICRPs and might be associated with local cytokine expression.
• Altered hepatic cytochrome P450 expression in cats after chronic exposure to decabromodiphenyl ether (BDE-209).
  Kraisiri Khidkhan; Hazuki Mizukawa; Yoshinori Ikenaka; Shouta M M Nakayama; Kei Nomiyama; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Shinsuke Tanabe; Mayumi Ishizuka
  The Journal of veterinary medical science, 82, 7, 978, 982, 31 Jul. 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The knowledge of cytochrome P450 (CYP) expression involved in chemical exposure are necessary in clinical applications for the medication and prediction of adverse effects. The aim of this study was to evaluate the mRNA expression of CYP1-CYP3 families in cats exposed to BDE-209 for one year. All selected CYP isoforms showed no significant difference in mRNA expressions between control and exposure groups, however, CYP3A12 and CYP3A131 revealed tend to be two times higher in the exposure group compared to control group. The present results indicate that the chronic exposure of BDE209 could not alter CYP expression in the liver of cats. This result considered caused by the deficiency of CYP2B subfamily which is major metabolism enzyme of polybrominated diphenyl ethers (PBDEs) in cat.
• An area ratio of thyroid gland to common carotid artery for evaluating the thyroid gland size.
  Noboru Sasaki; Noriyuki Nagata; Keitaro Morishita; Tatsuyuki Osuga; Kazuyoshi Sasaoka; Nozomu Yokoyama; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 82, 7, 1012, 1016, 31 Jul. 2020, [Domestic magazines]
  English, Scientific journal, The feasibility of ultrasonographic measurement of thyroid gland area to common carotid artery (TG:CCA) was investigated. Twenty-one healthy, 12 hypothyroid and 18 non-thyroid illness (NTI) dogs were evaluated. The area of thyroid lobe and common carotid artery in right and left sides were measured using the same ultrasonographic images in transverse plane. The average of the right and left ratio was calculated as TG:CCA. The median TG:CCA of 21 healthy dogs was 1.53, and it did not correlate either body weight or age. The median TG:CCA of 12 hypothyroid dogs was 0.81, which was significantly lower than that of 18 NTI dogs (1.81, P<0.001). If the cut off value <1.12 was used, TG:CCA indicated hypothyroidism with a sensitivity of 100%, specificity of 83%, and accuracy of 90%. Our data indicated that TG:CCA was independent of both body weight, which may contribute to consistent measurement of thyroid size. The results of this study suggest that TG:CCA is a promising tool for diagnosing canine hypothyroidism.
• Activities of matrix metalloproteinase-2, matrix metalloproteinase-9, and serine proteases in samples of the colorectal mucosa of Miniature Dachshunds with inflammatory colorectal polyps.
  Noriyuki Nagata; Hiroshi Ohta; Arisa Yamada; Yong Bin Teoh; Osamu Ichii; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 81, 7, 572, 580, Jul. 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVE: To investigate the activities of gelatinases (matrix metalloproteinase [MMP]-2 and MMP-9) and serine proteases in the colorectal mucosa of Miniature Dachshunds (MDs) with inflammatory colorectal polyps (ICRPs). ANIMALS: 15 MDs with ICRPs and 5 dogs with non-ICRP-related large bowel diarrhea (controls). PROCEDURES: Zymographic methods were used to evaluate the activities of MMP-2, MMP-9, latent forms of MMP-2 and MMP-9 (pro-MMP-2 and pro-MMP-9), and serine proteases in inflamed and noninflamed tissue samples from MDs with ICRPs and in noninflamed tissue samples from control dogs. The associations of serine protease activities with MMP-2 or MMP-9 activity were also analyzed. RESULTS: Activities of pro-MMP-2 and pro-MMP-9 were detected in most tissue samples, regardless of the tissue type, whereas activities of MMP-2 and MMP-9 were not detected in control tissue samples. In the inflamed tissue samples from MDs with ICRPs, the activities of MMP-2, pro-MMP-9, and MMP-9 were significantly higher than those in the noninflamed tissue samples from those dogs. Serine protease activities were significantly higher in the inflamed and noninflamed tissue samples from MDs with ICRP, compared with findings for control tissue samples. A weak correlation was detected between serine protease activities and MMP-9 activity. CONCLUSIONS AND CLINICAL RELEVANCE: Study results suggested that gelatinase and serine protease activities are upregulated in the colorectal mucosa of MDs with ICRPs, possibly contributing to the pathogenesis of this disease through the functions of these enzymes in degradation of extracellular matrix and promotion of inflammatory cell migration and inflammatory responses.
• Wobbly hedgehog syndrome with disseminated histiocytic sarcoma and lateral ventricular meningioma in an African pygmy hedgehog
  Lesa A. Thompson; Atsuya Morita; Shoko Murakami; Noboru Sasaki; Miou Murashita; Ryou Yamazaki; Atsushi Kobayashi; Takashi Kimura; Mitsuyoshi Takiguchi
  J Vet Diagn Invest, Jun. 2020, [Peer-reviewed]
  English, Scientific journal
• Altered hepatic cytochrome P450 expression in cats after chronic exposure to decabromodiphenyl ether (BDE-209).
  Kraisiri Khidkhan; Hazuki Mizukawa; Yoshinori Ikenaka; Shouta M M Nakayama; Kei Nomiyama; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Shinsuke Tanabe; Mayumi Ishizuka
  The Journal of veterinary medical science, 82, 7, 978, 982, 21 May 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The knowledge of cytochrome P450 (CYP) expression involved in chemical exposure are necessary in clinical applications for the medication and prediction of adverse effects. The aim of this study was to evaluate the mRNA expression of CYP1-CYP3 families in cats exposed to BDE-209 for one year. All selected CYP isoforms showed no significant difference in mRNA expressions between control and exposure groups, however, CYP3A12 and CYP3A131 revealed tend to be two times higher in the exposure group compared to control group. The present results indicate that the chronic exposure of BDE209 could not alter CYP expression in the liver of cats. This result considered caused by the deficiency of CYP2B subfamily which is major metabolism enzyme of polybrominated diphenyl ethers (PBDEs) in cat.
• Genome-wide DNA methylation analysis in canine gastrointestinal lymphoma.
  Hiroshi Ohta; Jumpei Yamazaki; Jaroslav Jelinek; Teita Ishizaki; Yumiko Kagawa; Nozomu Yokoyama; Noriyuki Nagata; Noboru Sasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 82, 5, 632, 638, 25 Mar. 2020, [Peer-reviewed], [Last author], [Domestic magazines]
  English, Scientific journal, DNA methylation is the covalent modification of methyl groups to DNA mostly at CpG dinucleotides and one of the most studied epigenetic mechanisms that leads to gene expression variability without affecting the DNA sequence. Genome-wide analysis of DNA methylation identified the signatures that could define subtypes of human lymphoma patients. The objective of this study was to conduct the genome-wide analysis of DNA methylation in dogs with gastrointestinal lymphoma (GIL). Genomic DNA was extracted from endoscopic biopsies from 10 dogs with GIL. We performed Digital Restriction Enzyme Assay of DNA Methylation (DREAM) for genome-wide DNA methylation analysis that could provide highly quantitative information on DNA methylation levels of CpG sites across the dog genome. We successfully obtained data of quantitative DNA methylation level for 148,601-162,364 CpG sites per GIL sample. Next, we analyzed 83,132 CpG sites to dissect the differences in DNA methylation between GIL and normal peripheral blood mononuclear cells (PBMCs). We found 383-3,054 CpG sites that were hypermethylated in GIL cases compared to PBMCs. Interestingly, 773 CpG sites including promoter regions of 61 genes were identified to be commonly hypermethylated in more than half of the cases, suggesting conserved DNA methylation patterns that are abnormal in GIL. This study revealed that there was a large number of hypermethylated sites that are common in most of canine GIL. These abnormal DNA methylation could be involved in tumorigenesis of the canine GIL.
• Long interspersed nucleotide element-1 hypomethylation in canine malignant mucosal melanoma.
  Teita Ishizaki; Jumpei Yamazaki; Shinji Meagawa; Nozomu Yokoyama; Keisuke Aoshima; Mitsuyoshi Takiguchi; Takashi Kimura
  Veterinary and comparative oncology, 18, 4, 854, 860, 18 Mar. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Canine malignant melanoma is a common cancer with a high mortality rate and is a clinically important disease. DNA methylation has been considered to be a potential tumorigenic mechanism through aberrant DNA methylation at promoter region which represses gene transcription. Global hypomethylation could also facilitate chromosome instability. There are few reports regarding DNA methylation in canine malignant melanoma; therefore, the purpose of this study was to examine DNA methylation status of long interspersed nucleotide element-1 (LINE-1) to be a surrogate marker of genome-wide methylation changes in this disease. We measured levels of DNA methylation of two adjacent cytosine-guanine sites on CpG island (CGI) at the putative promoter of canine LINE-1 sequence by bisulphite-pyrosequencing in 41 canine melanoma patient samples as well as six cell lines compared with normal mucosae. The survival rates were obtained from owners or medical records. We found DNA methylation levels of LINE-1 in normal mucosae were methylated. Interestingly, both melanoma cell lines and clinical melanoma samples showed remarkable hypomethylation. In addition, patients with lower LINE-1 methylation showed worse prognosis than those with higher LINE-1 methylation, though the difference did not reach statistical significance (P = .09). Here, we demonstrate that hypomethylation of LINE-1 is an epigenetically aberrant feature in canine melanoma with possible prognostic value.
• Clinical characteristics of dogs with food-responsive protein-losing enteropathy.
  Noriyuki Nagata; Hiroshi Ohta; Nozomu Yokoyama; Yong Bin Teoh; Khoirun Nisa; Noboru Sasaki; Tatsuyuki Osuga; Keitaro Morishita; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 34, 2, 659, 668, Mar. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: In dogs with protein-losing enteropathy (PLE), data on the clinical characteristics of food-responsive PLE (FR-PLE) remain scarce. OBJECTIVE: To determine the clinical characteristics of FR-PLE in dogs responsive to ultralow-fat diet (ULFD) management. ANIMALS: Thirty-three dogs diagnosed with PLE based on standard diagnostic criteria. METHODS: Retrospective review of medical records. Clinical findings were compared between dogs with FR-PLE (FR-PLE group) and those with immunosuppressant-responsive PLE (IR-PLE) or nonresponsive PLE (NR-PLE) (IR/NR-PLE group). The area under the curve (AUC) of a receiver operating characteristic curve was used to evaluate the ability of factors to differentiate the FR-PLE and IR/NR-PLE groups. Survival time was compared between the FR-PLE and IR/NR-PLE groups. RESULTS: Twenty-three dogs responded to ULFD management and were diagnosed with FR-PLE. The canine chronic enteropathy clinical activity index (CCECAI) was significantly lower in the FR-PLE group than in the IR/NR-PLE group (P < .001). The AUC of CCECAI for differentiating the FR-PLE group was 0.935 (95% confidence interval [CI], 0.845-1.000) with an optimal cutoff value of 8 (sensitivity, 0.826; specificity, 0.889). Survival times were significantly longer in the FR-PLE group (median, not reached) than in the IR/NR-PLE group (median, 432 days; P < .001). CONCLUSIONS AND CLINICAL IMPORTANCE: Dogs that respond to ULFD management and are diagnosed with FR-PLE are expected to have a favorable prognosis. Clinical scores, specifically the CCECAI, could be useful for differentiating FR-PLE from IR-PLE or NR-PLE.
• Computed tomographic features for differentiating benign from malignant liver lesions in dogs.
  Rommaneeya Leela-Arporn; Hiroshi Ohta; Genya Shimbo; Kiwamu Hanazono; Tatsuyuki Osuga; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 12, 1697, 1704, 18 Dec. 2019, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal, Thus far, there are few computed tomography (CT) characteristics that can distinguish benign and malignant etiologies. The criteria are complex, subjective, and difficult to use in clinical applications due to the high level of experience needed. This study aimed to identify practical CT variables and their clinical relevance for broadly classifying histopathological diagnoses as benign or malignant. In this prospective study, all dogs with liver nodules or masses that underwent CT examination and subsequent histopathological diagnosis were included. Signalments, CT findings and histopathological diagnoses were recorded. Seventy liver nodules or masses in 57 dogs were diagnosed, comprising 18 benign and 52 malignant lesions. Twenty-three qualitative and quantitative CT variables were evaluated using univariate and stepwise multivariate analyses, respectively. Two variables, namely, the postcontrast enhancement pattern of the lesion in the delayed phase (heterogeneous; odds ratio (OR): 14.7, 95% confidence interval (CI): 0.82-262.03, P=0.0429) and the maximal transverse diameter of the lesion (>4.5 cm; OR: 33.3, 95% CI: 2.29-484.18, P=0.0006), were significantly related to the differentiation of benign from malignant liver lesions, with an area under the curve of 0.8910, representing an accuracy of 88.6%. These findings indicate that features from triple-phase CT can provide information for distinguishing pathological varieties of focal liver lesions and for clinical decision making. Evaluations of the maximal transverse diameter and postcontrast enhancement pattern of the lesion included simple CT features for predicting liver malignancy with high accuracy in clinical settings.
• Tissue distribution and characterization of feline cytochrome P450 genes related to polychlorinated biphenyl exposure.
  Khidkhan K; Mizukawa H; Ikenaka Y; Nakayama SMM; Nomiyama K; Yokoyama N; Ichii O; Darwish WS; Takiguchi M; Tanabe S; Ishizuka M
  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 226, 108613, 108613, Dec. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Computed tomographic features for differentiating benign from malignant liver lesions in dogs.
  Leela-Arporn R; Ohta H; Shimbo G; Hanazono K; Osuga T; Morishita K; Sasaki N; Takiguchi M
  The Journal of veterinary medical science, 81, 12, 1697, 1704, Dec. 2019, [Peer-reviewed]
• Effects of PCB exposure on serum thyroid hormone levels in dogs and cats.
  Takaguchi K; Nishikawa H; Mizukawa H; Tanoue R; Yokoyama N; Ichii O; Takiguchi M; Nakayama SMM; Ikenaka Y; Kunisue T; Ishizuka M; Tanabe S; Iwata H; Nomiyama K
  The Science of the total environment, 688, 1172, 1183, Oct. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH-PCBs) might disrupt thyroid function. However, there is no clear evidence of PCB exposure disrupting thyroid hormone (TH) homeostasis in dogs and cats. The present study conducted in vivo experiments to evaluate the effects of a mixture of 12 PCB congeners (CB18, 28, 70, 77, 99, 101, 118, 138, 153, 180, 187 and 202, each congener 0.5 mg/kg BW, i.p. administration) on serum TH levels in male dogs (Canis lupus familiaris) and male cats (Felis silvestris catus). In PCB-exposed dogs, the time courses of higher-chlorinated PCBs and L-thyroxine (T4)-like OH-PCBs (4-OH-CB107 and 4-OH-CB202) concentrations were unchanged or tended to increase, whereas those of lower-chlorinated PCBs and OH-PCBs tended to decrease after 24 h. In PCB-exposed cats, concentrations of PCBs increased until 6 h and then remained unchanged. The levels of lower-chlorinated OH-PCBs including 4'-OH-CB18 increased until 96 h and then decreased. In PCB-exposed dogs, free T4 concentrations were higher than those in the control group at 48 and 96 h after PCB administration and positively correlated with the levels of T4-like OH-PCBs, suggesting competitive binding of T4 and T4-like OH-PCBs to a TH transporter, transthyretin. Serum levels of total T4 and total 3,3',5-triiodo-L-thyronine (T3) in PCB-exposed dogs were lower than in the control group at 24 and 48 h and negatively correlated with PCB concentrations, implying that PCB exposure enhanced TH excretion by increasing TH uptake and TH conjugation enzyme activities in the dog liver. In contrast, no obvious changes in TH levels were observed in PCB-exposed cats. This could be explained by the lower levels of T4-like OH-PCBs and lower hepatic conjugation enzyme activities in cats compared with dogs. Different effects on serum TH levels in PCB-exposed dogs and cats are likely to be attributable to species-specific PCB and TH metabolism.
• Change in right ventricular function in an American cocker spaniel with acute pulmonary thromboembolism.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Kiwamu Hanazono; Keitaro Morishita; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 81, 9, 1259, 1265, 03 Sep. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, A 12-year-old neutered female American cocker spaniel weighing 9.9 kg was presented for evaluation with a 2-day history of dyspnea and anorexia. Echocardiography revealed severe pulmonary hypertension (estimated systolic pulmonary arterial pressure, 93.4 mmHg) with right heart enlargement, pulmonary arterial dilation, and right ventricular dysfunction. The dilation of left heart and congenital cardiac shunt were not observed. Pulmonary thromboembolism (PTE) was confirmed by computed tomographic angiography. After treatment with antiplatelet and anticoagulant, the clinical sign and the echocardiographic abnormality of right heart were improved. These echocardiographic findings are not specific for PTE, but it can be useful as a rule-in test for PTE when other causes of pulmonary hypertension are excluded and a monitor of therapeutic efficacy.
• Usefulness of noninvasive shear wave elastography for the assessment of hepatic fibrosis in dogs with hepatic disease.
  Tamura M; Ohta H; Shimbo G; Osuga T; Sasaki N; Morishita K; Kagawa Y; Takiguchi M
  Journal of veterinary internal medicine, 33, 5, 2067, 2074, Sep. 2019, [Peer-reviewed], [Last author, Corresponding author]
  English
• Effect of ultra low fat diet on regulatory T cells of dogs with intestinal limphangiectasia secondary to chronic enteropathy: A pilot study
  Khoirun Nisa; Hiroshi Ohta; Nozomu Yokoyama; Masahiro Tamura; Noriyuki Nagata; Kazuyoshi Sasaoka; Angkhana Dermlim; Rommaneeya Leela-Arporn; Tomoya Morita; Tatsuyuki Osuga; Noboru Sasaki; Keitaro Morishita; Kensuke Nakamura; Yumiko Kagawa; Mitsuyoshi Takiguchi
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 67, 3, 221, 227, Aug. 2019, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Findings of contrast-enhanced ultrasonography with Sonazoid for cholangiocellular adenoma in three dogs.
  Tamura M; Nakamura K; Osuga T; Shimbo G; Sasaki N; Morishita K; Ohta H; Takiguchi M
  The Journal of veterinary medical science, 81, 8, 1104, 1108, Aug. 2019, [Peer-reviewed]
• Plasma amino acid profiles in dogs with inflammatory bowel disease.
  Yu Tamura; Hiroshi Ohta; Yumiko Kagawa; Tatsuyuki Osuga; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  Journal of veterinary internal medicine, 33, 4, 1602, 1607, Jul. 2019, [International Magazine]
  English, Scientific journal, BACKGROUND: Lymphocytic-plasmacytic enteritis is the common form of idiopathic inflammatory bowel disease (IBD) in dogs. In human IBD, disturbances of amino acid metabolism have been demonstrated to be involved in the pathophysiology of IBD. Therefore, plasma amino acid profile might represent a novel marker of human IBD. OBJECTIVES: To determine the plasma amino acid profiles of dogs with IBD and its usefulness as a novel marker of IBD in dogs. ANIMALS: Fasting blood plasma was obtained from 10 dogs with IBD and 12 healthy dogs. METHODS: All IBD dogs were prospectively included in this study, and heparinized blood samples were collected. The plasma concentrations of 21 amino acids were determined using the ninhydrin method. The relationships among the plasma amino acid concentrations and plasma C-reactive protein (CRP) concentration, canine chronic enteropathy clinical activity index (CCECAI), and overall World Small Animal Veterinary Association (WSAVA) score were investigated. RESULTS: Median concentration (nmol/mL) of methionine [46.2; range, 30.0-59.3], proline [119.4; range, 76.7-189.2], serine [115.1; range, 61.4-155.9], and tryptophan [17.4; range, 11.9-56.3]) were significantly lower than in control dogs [62.6; range, 51.0-83.6, 199.1; range, 132.5-376.7, 164.3; range, 124.7-222.9, and 68.3; range, 35.7-94.8, respectively]. A negative correlation was identified between the plasma serine concentration and CCECAI (r s = -.67, P = .03), but there were no correlations between plasma amino acid concentrations and CRP concentration or overall WSAVA score. CONCLUSIONS AND CLINICAL IMPORTANCE: Plasma serine concentration might represent a novel maker of IBD in dogs.
• Plasma-free amino acid profiles in dogs with hepatocellular carcinoma.
  Leela-Arporn R; Ohta H; Tamura M; Nagata N; Sasaoka K; Dermlim A; Nisa K; Osuga T; Morishita K; Sasaki N; Takiguchi M
  Journal of veterinary internal medicine, 33, 4, 1653, 1659, Jul. 2019, [Peer-reviewed]
• Effect of acute volume loading on left atrial strain values derived from two-dimensional speckle tracking echocardiography in dogs.
  Dermlim A; Osuga T; Nakamura K; Morita T; Nisa K; Sasaoka K; Leela-Arporn R; Nagata N; Tamura M; Sasaki N; Ohta H; Takiguchi M
  The Journal of veterinary medical science, 81, 7, 949, 957, Jul. 2019, [Peer-reviewed]
• Epidemiology of massive hepatocellular carcinoma in dogs: A 4-year retrospective study.
  Leela-Arporn R; Ohta H; Nagata N; Sasaoka K; Tamura M; Dermlim A; Nisa K; Morishita K; Sasaki N; Nakamura K; Takagi S; Hosoya K; Takiguchi M
  Veterinary journal (London, England : 1997), 248, 74, 78, Elsevier BV, Jun. 2019, [Peer-reviewed]
  Scientific journal
• Right ventricular function and dyssynchrony measured by echocardiography in dogs with precapillary pulmonary hypertension
  T. Morita; K. Nakamura; T. Osuga; K. Morishita; N. Sasaki; H. Ohta; M. Takiguchi
  Journal of Veterinary Cardiology, 23, 1, 14, Elsevier {BV}, Jun. 2019, [Peer-reviewed]
  Scientific journal
• The repeatability and left atrial strain analysis obtained via speckle tracking echocardiography in healthy dogs.
  Dermlim, A; Nakamura, K; Morita, T; Osuga, T; Nisa, K; Sasaoka, K; Leela-arporn, R; Sasaki, N; Ohta, H; Takiguchi, M
  Journal of Veterinary Cardiology, 23, 1, 69, 80, Elsevier BV, Jun. 2019, [Peer-reviewed]
  English, Scientific journal
• Predictive factors of malignancy in dogs with focal liver lesions using clinical data and ultrasonographic features.
  Leela-Arporn R; Ohta H; Tamura M; Nagata N; Sasaoka K; Dermlim A; Nisa K; Morishita K; Sasaki N; Nakamura K; Takagi S; Hosoya K; Takiguchi M
  The Journal of veterinary medical science, 81, 5, 723, 729, May 2019, [Peer-reviewed]
• Plasma amino acid profiles in dogs with inflammatory bowel disease.
  Tamura Y; Ohta H; Kagawa Y; Osuga T; Morishita K; Sasaki N; Takiguchi M
  Journal of veterinary internal medicine, May 2019, [Peer-reviewed]
• Evaluation of liver and spleen stiffness of healthy dogs by use of two-dimensional shear wave elastography.
  Tamura M; Ohta H; Nisa K; Osuga T; Sasaki N; Morishita K; Takiguchi M
  American journal of veterinary research, 80, 4, 378, 384, Apr. 2019, [Peer-reviewed], [Last author, Corresponding author]
• Evaluation of duodenal perfusion by contrast-enhanced ultrasonography in dogs with chronic inflammatory enteropathy and intestinal lymphoma.
  Nisa K; Lim SY; Shinohara M; Osuga T; Yokoyama N; Tamura M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 33, 2, 559, 568, Mar. 2019, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Contrast-enhanced ultrasonography is a feasible technique for quantifying hepatic microvascular perfusion in dogs with extrahepatic congenital portosystemic shunts.
  Tamura M; Ohta H; Nisa K; Osuga T; Sasaki N; Morishita K; Takiguchi M
  Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association, 60, 2, 192, 200, Mar. 2019, [Peer-reviewed]
• Low-intensity pulsed ultrasound modifies effects of a myelin-related growth inhibitor and enhances neurite re-growth
  Noboru Sasaki; Nobuki Kudo; Hiroshi Ohta; Mitsuyoshi Takiguchi
  2019 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS), 1308, 1310, 2019
  English, International conference proceedings
• Corrigendum: Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats.
  Ichii O; Ohta H; Horino T; Nakamura T; Hosotani M; Mizoguchi T; Morishita K; Nakamura K; Sasaki N; Takiguchi M; Sato R; Oyamada K; Elewa YHA; Kon Y
  Frontiers in veterinary science, 6, 2, 2019, [Peer-reviewed]
• Effect of acute volume overload on echocardiographic indices of right ventricular function and dyssynchrony assessed by use of speckle tracking echocardiography in healthy dogs.
  Morita T; Nakamura K; Osuga T; Yokoyama N; Morishita K; Sasaki N; Ohta H; Takiguchi M
  American journal of veterinary research, 80, 1, 51, 60, Jan. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE To determine the relationship between acute volume overload and echocardiographic indices of right ventricular (RV) function and dyssynchrony in healthy dogs. ANIMALS 7 healthy Beagles. PROCEDURES Right heart catheterization and echocardiography were performed in 7 healthy anesthetized Beagles at baseline and after induction of volume overload. Volume overload was induced by IV infusion of lactated Ringer solution (150 mL/kg/h for 90 minutes). Echocardiographic indices of RV function, including peak velocity of systolic tricuspid annular motion, tricuspid annulus plane systolic excursion, fractional area change, RV Tei index, RV longitudinal strain (RVLS), and systolic RV longitudinal strain rate (RVLSR), were obtained by use of speckle tracking echocardiography (STE). In addition, SD of the systolic shortening time of the right ventricle for the 6 segments (RV-SD6) was determined with STE. RESULTS Volume overload significantly increased the RV end-diastolic pressure, compared with the baseline value. Echocardiographic indices of RV function, except for septal RVLSR, were significantly enhanced by volume overload. In contrast, RV-SD6 did not change with volume overload. Although echocardiographic indices of RV function, except for septal RVLSR, were correlated with RV end-diastolic pressure, RV-SD6 was not correlated. CONCLUSIONS AND CLINICAL RELEVANCE Echocardiographic indices of RV function, including RVLS and RVLSR, were affected by acute short-term volume overload. Therefore, results for assessment of RV function by use of STE in dogs with clinical conditions associated with right-sided chronic volume overload, such as tricuspid and pulmonic regurgitation, should be interpreted with caution.
• Effects of immunosuppressive prednisolone therapy on pancreatic tissue and concentration of canine pancreatic lipase immunoreactivity in healthy dogs.
  Ohta H; Kojima K; Yokoyama N; Sasaki N; Kagawa Y; Hanazono K; Ishizuka T; Morishita K; Nakamura K; Takagi S; Takiguchi M
  Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 82, 4, 278, 286, Oct. 2018, [Peer-reviewed]
  English, Scientific journal
• ECG of the Month.
  Osuga T; Nakamura K; Morita T; Watanabe K; Takiguchi M
  Journal of the American Veterinary Medical Association, 253, 4, 417, 420, Aug. 2018, [Peer-reviewed]
• Pulmonary hypertension due to unclassified interstitial lung disease in a Pembroke Welsh corgi.
  Morita T; Nakamura K; Osuga T; Kobayashi A; Ichii O; Yabuki A; Takiguchi M
  The Journal of veterinary medical science, 80, 6, 939, 944, Jun. 2018, [Peer-reviewed]
• The effect of sedation with a combination of butorphanol and midazolam on quantitative contrast-enhanced ultrasonography of duodenum in healthy dogs.
  Nisa K; Lim SY; Osuga T; Yokoyama N; Tamura M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  The Journal of Veterinary Medical Science, 80, 3, 453, 459, Mar. 2018, [Peer-reviewed]
  English, Scientific journal
• The effect of sedation with a combination of butorphanol and midazolam on quantitative contrast-enhanced ultrasonography of duodenum in healthy dogs
  Nisa K; Lim S.Y; Osuga T; Yokoyama N; Tamura M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  Journal of Veterinary Medical Science, 80, 3, 453, 459, Mar. 2018, [Peer-reviewed]
  Scientific journal
• Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats.
  Ichii O; Ohta H; Horino T; Nakamura T; Hosotani M; Mizoguchi T; Morishita K; Nakamura K; Sasaki N; Takiguchi M; Sato R; Oyamada K; Elewa YHA; Kon Y
  Frontiers in veterinary science, 5, 289, 2018, [Peer-reviewed]
• Transcranial Doppler Ultrasound Examination in Dogs with Suspected Intracranial Hypertension Caused by Neurologic Diseases
  Sasaoka K; Nakamura K; Osuga T; Morita T; Yokoyama N; Morishita K; Sasaki N; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 32, 1, 314, 323, 2018, [Peer-reviewed]
  Scientific journal
• Intracellular diminazene aceturate content and adenosine incorporation in diminazene aceturate-resistant Babesia gibsoni isolate in vitro
  Masahiro Yamasaki; Nao Watanabe; Natsuki Idaka; Tohru Yamamori; Ken-ichi Otsuguro; Naohiro Uchida; Aiko Iguchi; Hiroshi Ohta; Mitsuyoshi Takiguchi
  EXPERIMENTAL PARASITOLOGY, 183, 92, 98, Dec. 2017, [Peer-reviewed]
  English, Scientific journal
• Case report: Congenital methemoglobinemia in a cat with the reduced NADH-cytochrome b5 reductase 3 activity and missense mutations in CYB5R3
  Akiyoshi Tani; Jumpei Yamazaki; Kensuke Nakamura; Mitsuyoshi Takiguchi; Mutsumi Inaba
  Japanese Journal of Veterinary Research, 65, 4, 201, 206, Faculty of Veterinary Medicine, Hokkaido University, Nov. 2017, [Peer-reviewed]
  English
• Spatial and temporal profile of cisplatin delivery by ultrasound-assisted intravesical chemotherapy in a bladder cancer model
  Noboru Sasaki; Kazuhiro Ishi; Nobuki Kudo; Shouta M. M. Nakayama; Kensuke Nakamura; Keitaro Morishita; Hiroshi Ohta; Mayumi Ishizuka; Mitsuyoshi Takiguchi
  PLOS ONE, 12, 11, e0188093, Nov. 2017, [Peer-reviewed]
  English, Scientific journal
• Diastolic heart failure associated with hemangiosarcoma infiltrating left ventricular walls in a dog
  Osuga T; Nakamura K; Morita T; Kagawa Y; Ohta H; Takiguchi M
  Canadian Veterinary Journal, 58, 11, 1167, 1170, Nov. 2017, [Peer-reviewed]
• Repeatability and reproducibility of quantitative contrast-enhanced ultrasonography for assessing duodenal perfusion in healthy dogs.
  Nisa K; Lim SY; Shinohara M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Yokoyama N; Osuga T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  The Journal of Veterinary Medical Science, 79, 9, 1585, 1590, Sep. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Contrast-enhanced ultrasonography (CEUS) with microbubbles as a contrast agent allows the visualization and quantification of tissue perfusion. The assessment of canine intestinal perfusion by quantitative CEUS may provide valuable information for diagnosing and monitoring chronic intestinal disorders. This study aimed to assess the repeatability (intraday variability) and reproducibility (interday variability) of quantitative duodenal CEUS in healthy dogs. Six healthy beagles underwent CEUS three times within one day (4-hr intervals) and on two different days (1-week interval). All dogs were sedated with a combination of butorphanol (0.2 mg/kg) and midazolam (0.1 mg/kg) prior to CEUS. The contrast agent (Sonazoid®) was administered using the intravenous bolus method (0.01 ml/kg) for imaging of the duodenum. Time-intensity curves (TIC) were created by drawing multiple regions of interest (ROIs) in the duodenal mucosa, and perfusion parameters, including the time-to-peak (TTP), peak intensity (PI), area under the curve (AUC), and wash-in and wash-out rates (WiR and WoR, respectively), were generated. Intraday and interday coefficients of variation (CVs) for TTP, PI, AUC, WiR and WoR were <25% (range, 2.27-23.41%), which indicated that CEUS was feasible for assessing duodenal perfusion in healthy sedated dogs. A further study of CEUS in dogs with chronic intestinal disorders is necessary to evaluate its clinical applicability.
• Repeatability and reproducibility of quantitative contrast-enhanced ultrasonography for assessing duodenal perfusion in healthy dogs
  Nisa K; Lim S.Y; Shinohara M; Nagata N; Sasaoka K; Dermlim A; Leela-Arporn R; Morita T; Yokoyama N; Osuga T; Sasaki N; Morishita K; Nakamura K; Ohta H; Takiguchi M
  Journal of Veterinary Medical Science, 79, 9, 1585, 1590, Sep. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit
  Yasunobu Arima; Takuto Ohki; Naoki Nishikawa; Kotaro Higuchi; Mitsutoshi Ota; Yuki Tanaka; Junko Nio-Kobayashi; Mohamed Elfeky; Ryota Sakai; Yuki Mori; Tadafumi Kawamoto; Andrea Stofkova; Yukihiro Sakashita; Yuji Morimoto; Masaki Kuwatani; Toshihihiko Iwanaga; Yoshichika Yoshioka; Naoya Sakamoto; Akihiko Yoshimura; Mitsuyoshi Takiguchi; Saburo Sakoda; Marco Prinz; Daisuke Kamimura; Masaaki Murakami
  ELIFE, 6, Aug. 2017, [Peer-reviewed]
  English, Scientific journal
• Changes in right ventricular function assessed by echocardiography in dog models of mild RV pressure overload
  Morita T; Nakamura K; Osuga T; Yokoyama N; Morishita K; Sasaki N; Ohta H; Takiguchi M
  Echocardiography, 34, 7, 1040, 1049, Jul. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Stimulation of colorectal biopsies from miniature dachshunds with inflammatory colorectal polyps with toll-like receptor ligands: A pilot study
  Yokoyama N; Ohta H; Kagawa Y; Nagata N; Nisa K; Morita T; Osuga T; Sasaki N; Morishita K; Nakamura K; Takiguchi M
  Veterinary Immunology and Immunopathology, 188, 78, 83, Jun. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Washout Ratio in the Hepatic Vein Measured by Contrast-Enhanced Ultrasonography to Distinguish Between Inflammatory and Noninflammatory Hepatic Disorders in Dogs
  K. Morishita; A. Hiramoto; A. Michishita; S. Takagi; T. Osuga; S. Y. Lim; K. Nakamura; N. Sasaki; H. Ohta; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 31, 3, 770, 777, May 2017, [Peer-reviewed]
  English, Scientific journal
• Washout Ratio in the Hepatic Vein Measured by Contrast-Enhanced Ultrasonography to Distinguish Between Inflammatory and Noninflammatory Hepatic Disorders in Dogs.
  Morishita K; Hiramoto A; Michishita A; Takagi S; Osuga T; Lim SY; Nakamura K; Sasaki N; Ohta H; Takiguchi M
  Journal of veterinary internal medicine, 31, 3, 770, 777, May 2017, [Peer-reviewed]
• Assessment of contrast-enhanced ultrasonography of the hepatic vein for detection of hemodynamic changes associated with experimentally induced portal hypertension in dogs
  Keitaro Morishita; Akira Hiramoto; Asuka Michishita; Satoshi Takagi; Yuki Hoshino; Takaharu Itami; Sue Yee Lim; Tatsuyuki Osuga; Sayuri Nakamura; Kenji Ochiai; Kensuke Nakamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 78, 4, 465, 471, Apr. 2017, [Peer-reviewed]
  English, Scientific journal
• Expression of apical junction complex proteins in colorectal mucosa of miniature dachshunds with inflammatory colorectal polyps
  Yokoyama N; Ohta H; Kagawa Y; Leela-Arporn R; Dermlim A; Nisa K; Morita T; Osuga T; Sasaki N; Morishita K; Nakamura K; Takiguchi M
  Journal of Veterinary Medical Science, 79, 3, 456, 463, Mar. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Contrast-enhanced ultrasonography of the hepatic vein in normal dogs.
  Morishita K; Hiramoto A; Osuga T; Lim SY; Khoirun N; Sasaki N; Nakamura K; Ohta H; Yamasaki M; Takiguchi M
  The Journal of veterinary medical science, 78, 12, 1753, 1758, Jan. 2017, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal, Contrast-enhanced ultrasonography (CEUS) of the hepatic vein can assess intrahepatic hemodynamic changes and has been studied as a noninvasive method to assess the severity of portal hypertension and hepatic fibrosis in humans. However, few reports have described its usefulness in veterinary medicine. The purpose of this study was to characterize CEUS findings of the hepatic vein in normal dogs and assess the repeatability of this method both in a conscious group (n=6) and a sedated group (n=6). Sonazoid® (0.01 ml/kg) was used as a contrast agent, and scanning of the hepatic vein was performed for 2 min. Time-intensity curves were generated from regions of interest in the hepatic vein. Four perfusion parameters were measured for quantitative analysis: hepatic vein arrival time (HVAT), time to peak (TTP), time to peak phase (TTPP) and wash-out ratio (WR). CEUS examinations were performed three times in each dog. The median (range) values of HVAT, TTP, TTPP and WR in the conscious group were 13.5 sec (9-22 sec), 12.5 sec (6-24 sec), 8 sec (6-13 sec) and 78.0% (60.7-91.7%), respectively. Median (range) values of HVAT, TTP, TTPP and WR in the sedated group were 12 sec (8-17 sec), 12.5 sec (9-17 sec), 9 sec (7-13 sec) and 84.1% (63.0-94.4%), respectively. The coefficients of variation of these parameters in the conscious and sedated groups were 7.6-29.7% and 11.8-14.8%, respectively.
• Impact of surgical margins on survival of 37 dogs with massive hepatocellular carcinoma
  A. Matsuyama; S. Takagi; K. Hosoya; Y. Kagawa; K. Nakamura; T. Deguchi; M. Takiguchi
  NEW ZEALAND VETERINARY JOURNAL, 65, 5, 227, 231, 2017, [Peer-reviewed]
  English, Scientific journal
• Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Yuki Hoshino; Satoshi Takagi; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yasuhiro Kon
  SCIENTIFIC REPORTS, 7, 40340, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• Serial measurement of pancreatic lipase immunoreactivity concentration in dogs with immune-mediated disease treated with prednisolone
  Ohta H; Morita T; Yokoyama N; Osuga T; Sasaki N; Morishita K; Nakamura K; Takiguchi M
  Journal of Small Animal Practice, 58, 6, 342, 347, 2017, [Peer-reviewed]
  Scientific journal
• Localization of Toll-like Receptor (TLR) 2 and TLR4 mRNA in the Colorectal Mucosa of Miniature Dachshunds with Inflammatory Colorectal Polyps
  Yokoyama N; Ohta H; Yamazaki J; Kagawa Y; Ichii O; Khoirun N; Morita T; Osuga T; Lim S.Y; Sasaki N; Morishita K; Nakamura K; Takiguchi M
  Journal of Comparative Pathology, 156, 2-3, 183, 190, 2017, [Peer-reviewed]
  Scientific journal
• Left Atrial Strain at Different Stages of Myxomatous Mitral Valve Disease in Dogs
  Nakamura K; Kawamoto S; Osuga T; Morita T; Sasaki N; Morishita K; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 31, 2, 316, 325, 2017, [Peer-reviewed]
• The repeatability and characteristics of right ventricular longitudinal strain imaging by speckle-tracking echocardiography in healthy dogs
  Morita T; Nakamura K; Osuga T; Yokoyama N; Khoirun N; Morishita K; Sasaki N; Ohta H; Takiguchi M
  Journal of Veterinary Cardiology, 19, 4, 351, 362, 2017, [Peer-reviewed]
  Scientific journal
• Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90.
  Masahiro Yamasaki; Yoshihiro Tsuboi; Yusuke Taniyama; Naohiro Uchida; Reeko Sato; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 78, 8, 1355, 60, 01 Sep. 2016, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length coding sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels were elevated when the parasites were incubated at 43°C for 1 hr.
• Effects of experimental cardiac volume loading on left atrial phasic function in healthy dogs
  Osuga T; Nakamura K; Morita T; Nisa K; Yokoyama N; Sasaki N; Morishita K; Ohta H; Takiguchi M
  American Journal of Veterinary Research, 77, 9, 952, 960, Sep. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Hypercalcemia in a Dog with Chronic Ingestion of Maxacalcitol Ointment.
  Nakamura K; Tohyama N; Yamasaki M; Ohta H; Morishita K; Takiguchi M
  Journal of the American Animal Hospital Association, 52, 4, 256, 258, Jul. 2016, [Peer-reviewed]
• Repeatability and reproducibility of right ventricular Tei index valves derived from three echocardiographic methods for evaluation of cardiac function in dogs
  Morita T; Nakamura K; Osuga T; Lim S.Y; Yokoyama N; Morishita K; Ohta H; Takiguchi M
  American Journal of Veterinary Research, 77, 7, 715, 720, Jul. 2016, [Peer-reviewed]
  Scientific journal
• Prognostic Value of Right Ventricular Tei Index in Dogs with Myxomatous Mitral Valvular Heart Disease
  Nakamura K; Morita T; Osuga T; Morishita K; Sasaki N; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 30, 1, 69, 75, Wiley-Blackwell, 2016, [Peer-reviewed]
  English
• Effects of various cardiovascular drugs on indices obtained with two-dimensional speckle tracking echocardiography of the left atrium and time-left atrial area curve analysis in healthy dogs
  Tatsuyuki Osuga; Kensuke Nakamura; Tomoya Morita; Sue Yee Lim; Nozomu Yokoyama; Keitaro Morishita; Hiroshi Ohta; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 76, 8, 702, 709, Aug. 2015, [Peer-reviewed]
  English, Scientific journal
• Quantitative contrast-enhanced ultrasonographic assessment of naturally occurring pancreatitis in dogs.
  Lim SY; Nakamura K; Morishita K; Sasaki N; Murakami M; Osuga T; Yokoyama N; Ohta H; Yamasaki M; Takiguchi M
  Journal of veterinary internal medicine / American College of Veterinary Internal Medicine, 29, 1, 71, 78, Jan. 2015, [Peer-reviewed]
  Scientific journal
• Vitamin D Status in Different Stages of Disease Severity in Dogs with Chronic Valvular Heart Disease
  Osuga T; Nakamura K; Morita T; Lim S.Y; Nisa K; Yokoyama N; Sasaki N; Morishita K; Ohta H; Takiguchi M
  Journal of Veterinary Internal Medicine, 29, 6, 1518, 1523, 2015, [Peer-reviewed]
  Scientific journal
• CONTRAST-ENHANCED ULTRASONOGRAPHIC FINDINGS IN THREE DOGS WITH PANCREATIC INSULINOMA
  Kensuke Nakamura; Sue-Yee Lim; Kenji Ochiai; Masahiro Yamasaki; Hiroshi Ohta; Keitaro Morishita; Satoshi Takagi; Mitsuyoshi Takiguchi
  VETERINARY RADIOLOGY & ULTRASOUND, 56, 1, 55, 62, Jan. 2015, [Peer-reviewed]
  English, Scientific journal
• Lesion Type Classification by Applying Machine-Learning Technique to Contrast-Enhanced Ultrasound Images
  Kazuya Takagi; Satoshi Kondo; Kensuke Nakamura; Mitsuyoshi Takiguchi
  IEICE TRANSACTIONS ON INFORMATION AND SYSTEMS, E97D, 11, 2947, 2954, Nov. 2014, [Peer-reviewed]
  English, Scientific journal
• Evaluation of Selected Cytokine Gene Expression in Colonic Mucosa from Dogs with Idiopathic Lymphocytic-plasmacytic Colitis
  Yu Tamura; Hiroshi Ohta; Nozomu Yokoyama; Sue Yee Lim; Tatsuyuki Osuga; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, 10, 1407, 1410, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• In vitro and in vivo safety and efficacy Studies of amphotericin B on Babesia gibsoni
  Masahiro Yamasaki; Eriko Harada; Yu Tamura; Sue Yee Lim; Tatsuyuki Ohsuga; Nozomu Yokoyama; Keitaro Morishita; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  VETERINARY PARASITOLOGY, 205, 3-4, 424, 433, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• Case report: Protein-losing enteropathy caused by Mesocestoides vogae (syn. M. corti) in a dog
  Yu Tamura; Hiroshi Ohta; Takuya Kashiide; Jun Matsumoto; Tatsuya Sakurai; Nozomu Yokoyama; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY PARASITOLOGY, 205, 1-2, 412, 415, Sep. 2014, [Peer-reviewed]
  English, Scientific journal
• First detection of Echinococcus multilocularis infection in two species of nonhuman primates raised in a zoo: A fatal case in Cercopithecus diana and a strongly suspected case of spontaneous recovery in Macaca nigra
  Kimiaki Yamano; Hirokazu Kouguchi; Kohji Uraguchi; Takeshi Mukai; Chikako Shibata; Hideaki Yamamoto; Noboru Takaesu; Masaki Ito; Yoshinori Makino; Mitsuyoshi Takiguchi; Kinpei Yagi
  PARASITOLOGY INTERNATIONAL, 63, 4, 621, 626, Aug. 2014, [Peer-reviewed]
  English, Scientific journal
• Expression of apical junction complex proteins in duodenal mucosa of dogs with inflammatory bowel disease
  Hiroshi Ohta; Yuji Sunden; Nozomu Yokoyama; Tatsuyuki Osuga; Sue Yee Lim; Yu Tamura; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 75, 8, 746, 751, Aug. 2014, [Peer-reviewed]
  English, Scientific journal
• Qualitative and Quantitative Contrast-Enhanced Ultrasonographic Assessment of Cerulein-Induced Acute Pancreatitis in Dogs
  S. Y. Lim; K. Nakamura; K. Morishita; N. Sasaki; M. Murakami; T. Osuga; H. Ohta; M. Yamasaki; M. Takiguchi
  JOURNAL OF VETERINARY INTERNAL MEDICINE, 28, 2, 496, 503, Mar. 2014, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• CD4(+) T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease
  Hiroshi Ohta; Kanae Takada; Yuji Sunden; Yu Tamura; Tatsuyuki Osuga; Sue Yee Lim; Masahiro Murakami; Noboru Sasaki; Bandula Kumara Wickramasekara Rajapakshage; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, 3, 409, 414, Mar. 2014, [Peer-reviewed]
  English, Scientific journal
• Ultrasound image-guided therapy enhances antitumor effect of cisplatin.
  Noboru Sasaki; Nobuki Kudo; Kensuke Nakamura; Sue Yee Lim; Masahiro Murakami; W R Bandula Kumara; Yu Tamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  Journal of medical ultrasonics (2001), 41, 1, 11, 21, Jan. 2014, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, PURPOSE: The aim of this study was to clarify whether ultrasound image-guided cisplatin delivery with an intratumor microbubble injection enhances the antitumor effect in a xenograft mouse model. METHODS: Canine thyroid adenocarcinoma cells were used for all experiments. Before in vivo experiments, the cisplatin and microbubble concentration and ultrasound exposure time were optimized in vitro. For in vivo experiments, cells were implanted into the back of nude mice. Observed by a diagnostic ultrasound machine, a mixture of cisplatin and ultrasound contrast agent, Sonazoid, microbubbles was injected directly into tumors. The amount of injected cisplatin and microbubbles was 1 μg/tumor and 1.2 × 10(7) microbubbles/tumor, respectively, with a total injected volume of 20 μl. Using the same diagnostic machine, tumors were exposed to ultrasound for 15 s. The treatment was repeated four times. RESULTS: The combination of cisplatin, microbubbles, and ultrasound significantly delayed tumor growth as compared with no treatment (after 18 days, 157 ± 55 vs. 398 ± 49 mm(3), P = 0.049). Neither cisplatin alone nor the combination of cisplatin and ultrasound delayed tumor growth. The treatment did not decrease the body weight of mice. CONCLUSION: Ultrasound image-guided anticancer drug delivery may enhance the antitumor effects of drugs without obvious side effects.
• Prognostic Value of Left Atrial Function in Dogs with Chronic Mitral Valvular Heart Disease
  Nakamura K; Osuga T; Morishita K; Suzuki S; Morita T; Yokoyama N; Ohta H; Yamasaki M; Takiguchi M
  Journal of Veterinary Internal Medicine, 28, 6, 1746, 1752, Wiley-Blackwell, 2014, [Peer-reviewed]
  English
• Qualitative and Quantitative Contrast Enhanced Ultrasonography of the Pancreas Using Bolus Injection and Continuous Infusion Methods in Normal Dogs
  Sue Yee Lim; Kensuke Nakamura; Keitaro Morishita; Noboru Sasaki; Masahiro Murakami; Tatsuyuki Osuga; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 75, 12, 1601, 1607, Dec. 2013, [Peer-reviewed]
  English, Scientific journal
• LC-MS/MS analysis of canine lipoproteins fractionated using the ultracentrifugation-precipitation method.
  Asuka Suto; Masahiro Yamasaki; Yukari Takasaki; Yasuyuki Fujita; Riichiro Abe; Hiroshi Shimizu; Hiroshi Ohta; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 75, 11, 1471, 7, Nov. 2013, [Peer-reviewed], [Domestic magazines]
  English, Due to the lack of a gold standard method in canine lipoprotein analysis, it is unclear whether canine high-density lipoprotein (HDL) and low-density lipoprotein (LDL) can be accurately evaluated by the lipoprotein analysis methods used for dogs. This study investigated whether the ultracentrifugation-precipitation (U-P) method was suitable as a gold standard method for analyzing canine lipoprotein. First, the U-P method was compared with a gel permeation high-performance liquid chromatography system (GP-HPLC). The concentrations of canine HDL cholesterol (HDL-C) and LDL cholesterol (LDL-C) determined by the U-P method correlated closely with those determined by GP-HPLC. However, the canine HDL-C concentration determined by the U-P method was lower than that determined by GP-HPLC, and the canine LDL-C concentration determined by the U-P method was higher than that determined by GP-HPLC. This study showed that some canine HDL could be precipitated with heparin manganese chloride solution. Second, the HDL and LDL fractions separated by the U-P method were analyzed by LC-MS/MS. The HDL fraction was found to contain only apolipoprotein A-I, which is an apolipoprotein of HDL, whereas the LDL fraction contained both apolipoprotein A-I and apolipoprotein B-100, which is an apolipoprotein of LDL. This data showed that a certain lipoprotein that includes apolipoprotein A-I might precipitate with canine LDL when using heparin manganese chloride solution. These results indicated that the U-P method is not currently a gold standard method for analyzing canine lipoproteins.
• Markedly increased expression of interleukin-8 in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds
  Yu Tamura; Hiroshi Ohta; Shidow Torisu; Masashi Yuki; Nozomu Yokoyama; Masahiro Murakami; Sue Yee Lim; Tatsuyuki Osuga; Keitaro Morishita; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 156, 1-2, 32, 42, Nov. 2013, [Peer-reviewed]
  English, Scientific journal
• Expression of CD4(+) T cell cytokine genes in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds
  Hiroshi Ohta; Kanae Takada; Shidow Torisu; Masashi Yuki; Yu Tamura; Nozomu Yokoyama; Tatsuyuki Osuga; Sue Yee Lim; Masahiro Murakami; Noboru Sasaki; Kensuke Nakamura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 155, 4, 259, 263, Oct. 2013, [Peer-reviewed]
  English, Scientific journal
• Increased intratumoral fluorothymidine uptake levels following multikinase inhibitor sorafenib treatment in a human renal cell carcinoma xenograft model
  Masahiro Murakami; Songji Zhao; Yan Zhao; Wenwen Yu; Chowdhury Nusrat Fatema; Ken-Ichi Nishijima; Masahiro Yamasaki; Mitsuyoshi Takiguchi; Nagara Tamaki; Yuji Kuge
  Oncology Letters, 6, 3, 667, 672, Sep. 2013, [Peer-reviewed]
  English, Scientific journal
• Repeatability and reproducibility of measurements obtained via two-dimensional speckle tracking echocardiography of the left atrium and time-left atrial area curve analysis in healthy dogs
  Tatsuyuki Osuga; Kensuke Nakamura; Sue Yee Lim; Yu Tamura; Wickramasekara Rajapakshage Bandula Kumara; Masahiro Murakami; Noboru Sasaki; Keitaro Morishita; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 74, 6, 864, 869, Jun. 2013, [Peer-reviewed]
  English, Scientific journal
• Evaluation of changes in the tumor microenvironment after sorafenib therapy by sequential histology and F-18-fluoromisonidazole hypoxia imaging in renal cell carcinoma
  Masahiro Murakami; Songji Zhao; Yan Zhao; Nusrat Fatema Chowdhury; Wenwen Yu; Ken-Ichi Nishijima; Mitsuyoshi Takiguchi; Nagara Tamaki; Yuji Kuge
  INTERNATIONAL JOURNAL OF ONCOLOGY, 41, 5, 1593, 1600, Nov. 2012, [Peer-reviewed]
  English, Scientific journal
• Link between inflammation and aquaporin-5 distribution in submandibular gland in sjögren's syndrome?
  M. S. Soyfoo; A. Konno; N. Bolaky; J. S. Oak; D. Fruman; C. Nicaise; M. Takiguchi; C. Delporte
  Oral Diseases, 18, 6, 568, 574, Sep. 2012, [Peer-reviewed]
  English, Scientific journal
• Involvement of Mitochondrial Genes of Babesia gibsoni in Resistance to Diminazene Aceturate
  Bandula Kumara Wickramasekara Rajapakshage; Masahiro Yamasaki; Shiang-Jyi Hwang; Noboru Sasaki; Masahiro Murakami; Yu Tamura; Sue Yee Lim; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 74, 9, 1139, 1148, Sep. 2012, [Peer-reviewed]
  English, Scientific journal
• Bilateral Segmental Aplasia with Unilateral Uterine Horn Torsion in a Pomeranian Bitch
  Kensuke Nakamura; Masahiro Yamasaki; Tomohiro Osaki; Hiroshi Ohta; Noboru Sasaki; Keisuke Aoshima; Takashi Kimura; Mitsuyoshi Takiguchi
  JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION, 48, 5, 327, 330, Sep. 2012, [Peer-reviewed]
  English, Scientific journal
• Analysis of energy generation and glycolysis pathway in diminazene aceturate-resistant Babesia gibsoni isolate in vitro
  Bandula Kumara Wickramasekara Rajapakshage; Masahiro Yamasaki; Masahiro Murakami; Yu Tamura; Sue Yee Lim; Tatsuyuki Osuga; Noboru Sasaki; Kensuke Nakamura; Hiroshi Ohta; Mitsuyoshi Takiguchi
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 60, 2-3, 51, 61, Aug. 2012, [Peer-reviewed]
  English, Scientific journal
• Enhanced Cytotoxic Effect of Cisplatin Using Diagnostic Ultrasound and Microbubbles In Vitro
  Sasaki Noboru; Nakamura Kensuke; Murakami Masahiro; Lim Sue Yee; Ohta Hiroshi; Yamasaki Masahiro; Takiguchi Mitsuyoshi
  11TH INTERNATIONAL SYMPOSIUM ON THERAPEUTIC ULTRASOUND, 1481, 307, 310, 2012, [Peer-reviewed]
• ACTIVATION OF MICROBUBBLES BY SHORT-PULSED ULTRASOUND ENHANCES THE CYTOTOXIC EFFECT OF CIS-DIAMMINEDICHLOROPLATINUM (II) IN A CANINE THYROID ADENOCARCINOMA CELL LINE IN VITRO
  Noboru Sasaki; Nobuki Kudo; Kensuke Nakamura; Sue Yee Lim; Masahiro Murakami; W. R. Bandula Kumara; Yu Tamura; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  ULTRASOUND IN MEDICINE AND BIOLOGY, 38, 1, 109, 118, Jan. 2012, [Peer-reviewed]
  English, Scientific journal
• Effects and Mechanisms of Action of Polyene Macrolide Antibiotic Nystatin on Babesia gibsoni In Vitro
  Masahiro Yamasaki; Norihisa Tamura; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Wickramasekara Rajapakshage; Bandula Kumara; Yu Tamura; Sue Yee Lim; Hiroshi Ohta; Mitsuyoshi Takiguchi
  JOURNAL OF PARASITOLOGY, 97, 6, 1190, 1192, Dec. 2011, [Peer-reviewed]
  English, Scientific journal
• Effects of sildenafil citrate on five dogs with Eisenmenger's syndrome
  K. Nakamura; M. Yamasaki; H. Ohta; N. Sasaki; M. Murakami; W. R. Bandula Kumara; M. Takiguchi
  JOURNAL OF SMALL ANIMAL PRACTICE, 52, 11, 595, 598, Nov. 2011, [Peer-reviewed]
  English, Scientific journal
• Pathological correlations between podocyte injuries and renal functions in canine and feline chronic kidney diseases.
  Ichii O; Yabuki A; Sasaki N; Otsuka S; Ohta H; Yamasaki M; Takiguchi M; Namiki Y; Hashimoto Y; Endoh D; Kon Y
  Histology and histopathology, 26, 10, 1243, 1255, Universidad de Murcia, Oct. 2011, [Peer-reviewed]
  English, Podocytes cover the glomerulus and their adjacent foot processes form a principal barrier called the slit diaphragm. Podocyte dysfunctions, including podocyte loss and slit diaphragm disruptions, induce chronic kidney diseases (CKD). In this study, we analyzed the correlations between podocyte injuries and renal dysfunctions in domestic carnivores. Dogs and cats were divided into normal and CKD groups according to renal histopathology and plasma creatinine values. Immunostaining results showed that linear reactions of slit diaphragm molecules, e.g., nephrin, podocin, and ACTN4, were parallel to glomerular capillaries in all animals. However, in dogs, reactions of nephrin and ACTN4 were changed to a granular pattern in the CKD group, and their intensities significantly decreased with the number of podocytes in the glomerulus. Moreover, the expression of nephrin and ACTN4 negatively correlated with creatinine. Real-time PCR analysis showed that nephrin mRNA expression in the kidneys of CKD dogs was significantly lower than that in normal animals, and negatively correlated with creatinine. Although no significant correlation between renal dysfunction and podocyte injury was detected in cats, histoplanimetric scores of tubulointerstitial lesions in CKD cats were higher than those in both normal cats and diseased dogs. Furthermore, mRNAs of WT1 and SD molecules were detected in urine from CKD animals. In conclusion, podocyte injuries such as podocytopenia and decreased expression of nephrin and ACTN4 in the glomerulus were more strongly correlated with renal dysfunction in dogs than in cats. These findings suggest that the CKD pathogenesis, especially susceptibilities to podocyte injuries, differed between dogs and cats.
• Detection of anti-Babesia gibsoni heat shock protein 70 antibody and anti-canine heat shock protein 70 antibody in sera from Babesia gibsoni-infected dogs
  Masahiro Yamasaki; Mikiko Ishida; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Wickramasekara Rajapakshage Bandula Kumara; Yu Tamura; Sue Yee Lim; Hiroshi Ohta; Mitsuyoshi Takiguchi
  VETERINARY PARASITOLOGY, 180, 3-4, 215, 225, Aug. 2011, [Peer-reviewed]
  English, Scientific journal
• Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa
  Hiroshi Ohta; Tomoki Yamaguchi; B. K. Wickramasekara Rajapakshage; Masahiro Murakami; Noboru Sasaki; Kensuke Nakamura; Shiang-Jyi Hwang; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  AMERICAN JOURNAL OF VETERINARY RESEARCH, 72, 8, 1046, 1051, Aug. 2011, [Peer-reviewed]
  English, Scientific journal
• Altered expression of miR-146a correlates with the development of chronic renal inflammation.
  Ichii O; Yabuki A; Sasaki N; Otsuka S; Ohta H; Yamasaki M; Takiguchi M; Namiki Y; Hashimoto Y; Endoh D; Kon Y
  Kidney Int., 81, 3, 280, 292, 2011, [Peer-reviewed]
  English, Scientific journal
• Babesia gibsoni: Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis
  Masahiro Yamasaki; Yusuke Kobayashi; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Bandula Kumara Wickramasekara Rajapakshage; Hiroshi Ohta; Osamu Yamato; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  EXPERIMENTAL PARASITOLOGY, 127, 1, 119, 126, Jan. 2011, [Peer-reviewed]
  English, Scientific journal
• Contrast-enhanced ultrasonography for characterization of focal splenic lesions in dogs.
  Nakamura K; Sasaki N; Murakami M; Bandula Kumara WR; Ohta H; Yamasaki M; Takagi S; Osaki T; Takiguchi M
  Journal of veterinary internal medicine / American College of Veterinary Internal Medicine, 24, 6, 1290, 1297, 6, Nov. 2010, [Peer-reviewed]
  Nakamura K, Sasaki N, Murakami M, Bandula Kumara WR, Ohta H, Yamasaki M, Takagi S, Osaki T, Takiguchi M, <i>Journal of veterinary internal medicine / American College of Veterinary Internal Medicine</i>, 2010, vol. 24, no. 6, pp. 1290-1297
• Reduced transcript levels of the heat shock protein 70 gene in diminazene aceturate-resistant Babesia gibsoni variants under low concentrations of diminazene aceturate
  Shiang-Jyi Hwang; Masahiro Yamasaki; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Bandula Kumara Wickramasekara Rajapakshage; Hiroshi Ohta; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 58, 3-4, 155, 164, Nov. 2010, [Peer-reviewed]
  English, Scientific journal
• Development and Characterization of a Strain of Babesia gibsoni Resistant to Diminazene Aceturate In Vitro
  Shiang-Jyi Hwang; Masahiro Yamasaki; Kensuke Nakamura; Noboru Sasaki; Masahiro Murakami; Bandula Kumara Wickramasekara Rajapakshage; Hiroshi Ohta; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 72, 6, 765, 771, Jun. 2010, [Peer-reviewed]
  English, Scientific journal
• Local overexpression of interleukin-1 family, member 6 relates to the development of tubulointerstitial lesions
  Osamu Ichii; Saori Otsuka; Nobuya Sasaki; Akira Yabuki; Hiroshi Ohta; Mitsuyoshi Takiguchi; Yoshiharu Hashimoto; Daiji Endoh; Yasuhiro Kon
  LABORATORY INVESTIGATION, 90, 3, 459, 475, Mar. 2010, [Peer-reviewed]
  English, Scientific journal
• CONTRAST-ENHANCED ULTRASONOGRAPHY FOR CHARACTERIZATION OF CANINE FOCAL LIVER LESIONS
  Kensuke Nakamura; Satoshi Takagi; Noboru Sasaki; Wickramasekara Rajapakshage Bandula Kumara; Masahiro Murakami; Hiroshi Ohta; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY RADIOLOGY & ULTRASOUND, 51, 1, 79, 85, Jan. 2010, [Peer-reviewed]
  English, Scientific journal
• EFFECTS AND MECHANISMS OF ACTION OF IONOPHOROUS ANTIBIOTICS VALINOMYCIN AND SALINOMYCIN-NA ON BABESIA GIBSONI IN VITRO
  Masahiro Yamasaki; Kensuke Nakamura; Norihisa Tamura; Shiang-Jyi Hwang; Muneyoshi Yoshikawa; Noboru Sasaki; Hiroshi Ohta; Osamu Yamato; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  JOURNAL OF PARASITOLOGY, 95, 6, 1532, 1538, Dec. 2009, [Peer-reviewed]
  English, Scientific journal
• Primary Splenic Torsion in a Boston Terrier
  Hiroshi Ohta; Satoshi Takagi; Masahiro Murakami; Noboru Sasaki; Muneyoshi Yoshikawa; Kensuke Nakamura; Shiang-Jyi Hwang; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 71, 11, 1533, 1535, Nov. 2009, [Peer-reviewed]
  English, Scientific journal
• QUANTITATIVE CONTRAST-ENHANCED ULTRASONOGRAPHY OF CANINE SPLEEN
  Kensuke Nakamura; Noboru Sasaki; Muneyoshi Yoshikawa; Hiroshi Ohta; Shiang-Jyi Hwang; Takahiro Mimura; Masahiro Yamasaki; Mitsuyoshi Takiguchi
  VETERINARY RADIOLOGY & ULTRASOUND, 50, 1, 104, 108, Jan. 2009, [Peer-reviewed]
  English, Scientific journal
• Flow cytometry to evaluate the level of Babesia gibsoni parasitemia in vivo and in vitro by using the fluorescent nucleic acid stain SYTO16
  Masahiro Yamasaki; Shiang-Jyi Hwang; Hiroshi Ohta; Osamu Yamato; Yoshimitsu Maede; Mitsuyoshi Takiguchi
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 55, 4, 129, 136, Feb. 2008, [Peer-reviewed]
  English, Scientific journal
• Identification of a quantitative trait locus regulating B cell-dominant infiltration into autoimmune sialitis lesions of the IQI mouse model of primary Sjogren's syndrome
  Akihiro Konno; Mitsuyoshi Takiguchi; Kensuke Takada; Takeshi Usami; Kaoru Azumi; Hisayo Kubota; Mutsumi Inaba; Junzo Saegusa; Yasuhiro Kon
  IMMUNOGENETICS, 59, 11, 853, 859, Nov. 2007, [Peer-reviewed]
  English, Scientific journal
• Restricted localization of claudin-16 at the tight junction in the thick ascending limb of Henle's loop together with claudins 3, 4, and 10 in bovine nephrons
  H Ohta; H Adachi; M Takiguchi; M Inaba
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, 5, 453, 463, May 2006, [Peer-reviewed]
  English
• Magnetic resonance imaging of alveolar echinococcosis experimentally induced in the rat lung
  T Asanuma; T Kawahara; O Inanami; M Nakao; K Nakaya; A Ito; M Takiguchi; A Hashimoto; M Kuwabara
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, 1, 15, 20, Jan. 2006, [Peer-reviewed]
  English
• Different effects on the inflammatory lesions in the lacrimal and salivary glands after neonatal thymectomy in IQI/Jic mice, a model for Sjogren's syndrome
  K Takada; M Takiguchi; M Inaba
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 9, 955, 957, Sep. 2005, [Peer-reviewed]
  English
• Autoimmunity against a tissue kallikrein in IQI/Jic mice - A model for Sjogren's syndrome
  K Takada; M Takiguchi; A Konno; A Inaba
  JOURNAL OF BIOLOGICAL CHEMISTRY, 280, 5, 3982, 3988, Feb. 2005, [Peer-reviewed]
  English, Scientific journal
• Diagnostic ultrasound of polypoid cystitis in dogs
  M Takiguchi; M Inaba
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 1, 57, 61, Jan. 2005, [Peer-reviewed]
  English
• Magnetic resonance imaging and immunoblot analyses in rats with experimentally induced cerebral alveolar echinococcosis
  T Asanuma; Y Matsumoto; M Takiguchi; O Inanami; M Nakao; K Nakaya; A Ito; A Hashimoto; M Kuwabara
  COMPARATIVE MEDICINE, 53, 6, 649, 656, Dec. 2003, [Peer-reviewed]
  English, Scientific journal
• Presence of B7-2+ dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjögren's syndrome
  Akihiro Konno; Kensuke Takada; Junzo Saegusa; Mitsuyoshi Takiguchi
  Autoimmunity, 36, 4, 247, 254, Jun. 2003, [Peer-reviewed]
  English, Scientific journal
• Virologic and serologic identification of minute virus of canines (canine parvovirus type 1) from dogs in Japan
  M Mochizuki; M Hashimoto; T Hajima; M Takiguchi; A Hashimoto; Y Une; F Roerink; T Ohshima; CR Parrish; LE Carmichael
  JOURNAL OF CLINICAL MICROBIOLOGY, 40, 11, 3993, 3998, Nov. 2002, [Peer-reviewed]
  English, Scientific journal
• Detection of Canine Herpesvirus DNA in the Ganglionic Neurons and the Lymph Node Lymphocytes of Latently Infected Dogs
  Masahiro Miyoshi; Yuki Ishii; Mitsuyoshi Takiguchi; Ayato Takada; Jun Yasuda; Akira Hashimoto; Katsunori Okazaki; Hiroshi Kida
  Journal of Veterinary Medical Science, 61, 4, 375, 379, Apr. 1999, [Peer-reviewed]
  English, Scientific journal
• イヌヘルペスウイルスの神経節神経細胞及びリンパ節リンパ球における潜伏感染
  三好 正浩; 石井 由紀; 滝口 満喜; 高田 礼人; 安田 隼; 橋本 晃; 岡崎 克則; 喜田 宏
  The Journal of Veterinary Medical Science, 61, 4, 375, 379, (公社)日本獣医学会, Apr. 1999, [Peer-reviewed]
  English

• 新時代の小動物臨床医学 内科学におけるブレイクスルー
  滝口 満喜, 日本獣医学会学術集会講演要旨集, 168回, 155, 155, Sep. 2025
  (公社)日本獣医学会, Japanese
• 低温プラズマ照射溶液による膀胱癌株細胞に対する凝細胞効果の検討
  佐々木 東; 安井 博宣; 田中 宏昌; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 168回, 304, 304, Sep. 2025
  (公社)日本獣医学会, Japanese
• LC/Q-TOF/MSを用いたメタボロミクスによる犬大細胞性消化器型リンパ腫の血清バイオマーカー探索
  澤村 飛龍; 横山 望; 松山 楓; 池中 良徳; 山崎 淳平; 菅原 芽伊[須田]; 笹岡 一慶; 森下 啓太郎; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 168回, 307, 307, Sep. 2025
  (公社)日本獣医学会, Japanese
• 犬の肝臓におけるSuperb Microvascular Imagingの検者間信頼性の検討
  本田 英俊; 横山 望; 澤村 飛龍; 菅原 芽伊[須田]; 川元 誠; 新坊 弦也; 笹岡 一慶; 佐々木 東; 森下 啓太郎; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 168回, 309, 309, Sep. 2025
  (公社)日本獣医学会, Japanese
• 犬の僧帽弁粘液腫様変性における血管内皮障害バイオマーカーSyndecan-1濃度に影響する因子の探索
  井上 祐人; 田村 純; 細谷 謙次; 金 尚昊; 山崎 淳平; 菅原 芽伊; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 168回, 312, 312, Sep. 2025
  (公社)日本獣医学会, Japanese
• 犬における経頭蓋超音波による脳および脳血管の描出性の検討
  昇 良樹; 笹岡 一慶; 新坊 弦也; 横山 望; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 168回, 313, 313, Sep. 2025
  (公社)日本獣医学会, Japanese
• 若齢期からの慢性的な運動失調を呈し、拡散テンソル画像(DTI)を用いて脳白質の萎縮を定量的に評価した猫の1例
  中村 伽奈; 笹岡 一慶; 新坊 弦也; 山根 由久; 中村 健介; 滝口 満喜, 北海道獣医師会雑誌, 69, 8, 355, 355, Aug. 2025
  (公社)北海道獣医師会, Japanese
• 行動異常を主訴としたフレンチブルドッグの大脳膠腫症の1例
  加藤 史; 笹岡 一慶; 新坊 弦也; 越川 藍; 木村 享史; 滝口 満喜, 北海道獣医師会雑誌, 69, 8, 355, 355, Aug. 2025
  (公社)北海道獣医師会, Japanese
• 耳科処置により医原性気脳を発症した犬の1例
  川上 侑記; 笹岡 一慶; 田村 純; 大山 紀彦; 新坊 弦也; 山根 由久; 横山 望; 中村 健介; 滝口 満喜, 北海道獣医師会雑誌, 69, 8, 356, 356, Aug. 2025
  (公社)北海道獣医師会, Japanese
• 獣医学教育先進地域としての役割と課題 北海道大学の獣医学教育の概要と課題
  滝口 満喜, 北海道獣医師会雑誌, 69, 8, 380, 380, Aug. 2025
  (公社)北海道獣医師会, Japanese
• 肺高血圧症を併発した粘液腫様変性僧帽弁疾患の犬に対する僧帽弁形成術の短期治療成績
  川元誠; 川元誠; 中村健介; 塩原希; 佐藤景; 村田芙花; 池田正悟; 滝口満喜, 北海道獣医師会雑誌, 69, 8, 360, 360, 2025
  (公社)北海道獣医師会, Japanese
• 持続性心房静止と診断した犬の1例
  塩原希; 中村健介; 川元誠; 滝口満喜, 北海道獣医師会雑誌, 69, 8, 360, 360, 2025
  (公社)北海道獣医師会, Japanese
• 意識障害は起源不明髄膜脳脊髄炎(MUO)の予後不良に関連する MUO82例の回顧的検討(2014～2022)
  笹岡 一慶; 福家 知樹; 須永 隆文; 大田 寛; 滝口 満喜, 北海道獣医師会雑誌, 68, 8, 313, 313, Aug. 2024
  (公社)北海道獣医師会, Japanese
• ラブラドール・レトリーバーのコロニーで集団発生したサルファ剤過敏症とCYPB5Rの遺伝子型頻度解析
  岸原 果子; 横山 望; 山崎 淳平; 笹岡 一慶; 森下 啓太郎; 中村 健介; 宮原 和郎; 近藤 厚; 滝口 満喜; 高橋 徹, 北海道獣医師会雑誌, 68, 8, 314, 314, Aug. 2024
  (公社)北海道獣医師会, Japanese
• 慢性リンパ球性白血病により大細胞性リンパ腫がマスクされていた犬の1例
  塩原 希; 森下 啓太郎; 五日市 大介; 後藤 裕子; 富安 博隆; 滝口 満喜, 北海道獣医師会雑誌, 68, 8, 317, 317, Aug. 2024
  (公社)北海道獣医師会, Japanese
• 気管支拡張症の原因として気管支軟骨の低形成が疑われた2頭のアメリカン・コッカー・スパニエル
  川上 侑記; 中村 健介; 菅原 芽伊; 新坊 弦也; 山根 由久; 細谷 謙次; 岡田 一喜; 賀川 由美子; 滝口 満喜, 北海道獣医師会雑誌, 68, 8, 321, 321, Aug. 2024
  (公社)北海道獣医師会, Japanese
• 部分肺静脈還流異常により右心拡大を呈した犬の1例
  安田 理紗; 中村 健介; 新坊 弦也; 横山 望; 滝口 満喜, 北海道獣医師会雑誌, 68, 8, 323, 323, Aug. 2024
  (公社)北海道獣医師会, Japanese
• 低強度パルス超音波による培養神経細胞刺激作用の検討
  佐々木 東; 工藤 信樹; 滝口 満喜, 超音波医学, 51, Suppl., S672, S672, Apr. 2024
  (公社)日本超音波医学会, Japanese
• モデル犬を用いた肺高血圧症を診断するための心エコー図指標の有用性の検証
  森田智也; 森田智也; 中村健介; 大菅辰幸; 川元誠; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 121st, 2024
• 猫の心筋症における左房ブースターストレインの有用性の検討
  佐藤景; 中村健介; 川元誠; 菅原芽伊; 笹岡一慶; 横山望; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 121st, 2024
• 患者腫瘍組織移植モデル(PDX)を用いた犬の前立腺癌・移行上皮癌に対するダブラフェニブの有効性の評価
  大橋咲子; 木之下怜平; 木之下怜平; 青島圭佑; 山尾結衣; 山崎淳平; 金尚昊; 細谷謙次; 山崎淳平; 金尚昊; 細谷謙次; 滝口満喜, 日本獣医学会学術集会講演要旨集, 167th, 2024
• 大動脈小体腫瘍の心膜転移により収縮性心膜炎を呈した犬の1例
  塩原希; 中村健介; 細谷謙次; 新坊弦也; 賀川由美子; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 120th, 2024
• 殺細胞効果を有する液体の膀胱内注入治療法の検討
  佐々木東; 池中良徳; 安井博宣; 中村健介; 滝口満喜, 日本獣医学会学術集会講演要旨集, 167th, 288, 288, 2024
  (公社)日本獣医学会, Japanese
• 乳び胸の原因としてリンパ管腫症が疑われた犬の1例
  早坂 郁人; 新坊 弦也; 細谷 謙次; 三木 伸悟; 滝口 満喜, 日本獣医画像診断学会抄録, 73回, np176, np176, Dec. 2023
  (一社)日本獣医画像診断学会, Japanese
• 標準的感染症対策プログラムは動物病院において手指衛生コンプライアンス向上と多剤耐性菌出現抑制に貢献する
  笹岡 一慶; 森下 啓太郎; 細谷 謙次; 佐藤 敬近; 佐藤 豊孝; 堀内 基広; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 166回, 174, 174, Sep. 2023
  (公社)日本獣医学会, Japanese
• 犬の腎うっ血の評価におけるShear wave elastographyの有用性の検討
  塩原 希; 中村 健介; 横山 望; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 67, 8, 316, 316, Aug. 2023
  (公社)北海道獣医師会, Japanese
• 拘束型心筋症に伴う左室内血栓により大動脈血栓塞栓に陥った猫の1例
  立花 圭; 中村 健介; 塩原 希; 川上 侑記; 滝口 満喜, 北海道獣医師会雑誌, 67, 8, 318, 318, Aug. 2023
  (公社)北海道獣医師会, Japanese
• 犬の発作性ジスキネジア23例の回顧的検討
  川上 侑記; 笹岡 一慶; 大田 寛; 山根 由久; 新坊 弦也; 横山 望; 佐々木 東; 森下 啓太郎; 中村 健介; 滝口 満喜, 北海道獣医師会雑誌, 67, 8, 316, 316, Aug. 2023
  (公社)北海道獣医師会, Japanese
• コンパニオンアニマルのPFAS汚染:イヌ・ネコの種差要因と汚染の地域差
  佐藤 愛佳; 佐藤 楓夏; 水川 葉月; 池中 良徳; 田上 瑠美; 横山 望; 滝口 満喜; 中津 賞; 野見山 桂, 環境化学物質3学会合同大会要旨集, 2回, 305, 306, May 2023
  (一社)日本環境化学会・日本環境毒性学会・環境ホルモン学会, Japanese
• 胃・小腸の拡張—特集 画像所見からみる診断のアプローチ
  横山 望; 滝口 満喜, 伴侶動物画像診断 = Diagnostic imaging of companion animal, 8, 2, 6, 32, Apr. 2023
  緑書房, Japanese
• 造影超音波検査で副腎皮質の血流消失を確認した医原性副腎皮質機能低下症の犬の1例
  永田矩之; 前田貞俊; 青島圭佑; 中村健介; 滝口満喜, 日本獣医師会獣医学術学会年次大会講演要旨集, 2023 (Web), 2023
• 犬においてピモベンダンとドブタミンを併用することにはどのような意味があるのか?
  大菅辰幸; 大菅辰幸; 矢口紗羅; 中村健介; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 118th, 2023
• 心疾患の犬におけるShear wave elastography/Dispersion Imagingの有用性の検討
  塩原希; 中村健介; 横山望; 佐々木東; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 119th, 2023
• 造影超音波検査で副腎皮質の血流消失を確認した医原性副腎皮質機能低下症の犬の1例
  永田矩之; 前田貞俊; 青島圭佑; 中村健介; 滝口満喜, 日本産業動物獣医学会(中部)・日本小動物獣医学会(中部)・日本獣医公衆衛生学会(中部), 2023, 2023
• 容量過負荷状態の犬における心エコー図法による左心房stiffnessの推定
  大菅 辰幸; 田村 昌大; 佐々木 東; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 165回, [HS1P, 05], Sep. 2022
  (公社)日本獣医学会, Japanese
• 2次診療動物病院に来院した犬の糞便真核生物叢解析
  横山 望; 川口 剛; 中尾 亮; 森下 啓太郎; 細谷 謙次; 永田 矩之; 笹岡 一慶; 新坊 弦也; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 165回, [HS1P, 16], Sep. 2022
  (公社)日本獣医学会, Japanese
• 容量過負荷状態の犬における心エコー図法による左心房stiffnessの推定
  大菅 辰幸; 田村 昌大; 佐々木 東; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 165回, [HS1P, 05], Sep. 2022
  (公社)日本獣医学会, Japanese
• 2次診療動物病院に来院した犬の糞便真核生物叢解析
  横山 望; 川口 剛; 中尾 亮; 森下 啓太郎; 細谷 謙次; 永田 矩之; 笹岡 一慶; 新坊 弦也; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 165回, [HS1P, 16], Sep. 2022
  (公社)日本獣医学会, Japanese
• 犬尿路上皮癌におけるPDXモデルの樹立
  森下 大暉; 木之下 怜平; 青島 圭佑; 細谷 謙次; 山崎 淳平; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 165回, [B2P, 03], Sep. 2022
  (公社)日本獣医学会, Japanese
• 磁気共鳴スペクトロスコピーは犬の起源不明髄膜脳脊髄炎の予後指標となり得るか
  山口 敬央; 笹岡 一慶; 新坊 弦也; 山根 由久; 永田 矩之; 横山 望; 森下 啓太郎; 佐々木 東; 中村 健介; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 287, 287, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 造影CT検査後に非心原性肺水腫を呈した褐色細胞腫を疑う犬の1例
  吉田 紫乃; 田村 純; 永田 矩之; 大山 紀彦; 新坊 弦也; 池中 良徳; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 289, 289, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 麻酔中パルスオキシメーター測定異常から舌局所の灌流障害を疑った犬の1例
  田村 純; 湯本 優希; 大山 紀彦; 新坊 弦也; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 289, 289, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 低コレステロール血症は犬の膵外分泌不全を予測できるのか?
  横山 望; 大田 寛; 永田 矩之; 笹岡 一慶; 新坊 弦也; 佐々木 東; 森下 啓太郎; 中村 健介; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 284, 284, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 洞停止を認めた咳嗽失神の犬の1例
  幸野 瑠美; 中村 健介; 笹岡 一慶; 塩原 希; 小幡 祐路; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 284, 284, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 不完全型房室中隔欠損症の外科的整復時に両側房室弁の弁輪形成術を行った犬の1例
  川元 誠; 中村 健介; 森 拓也; 古越 真耶; 大橋 美里; 三木 悠矢; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 285, 285, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 甲状腺機能低下症の治療により失神の消失を認めた犬の1例
  塩原 希; 中村 健介; 幸野 瑠美; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 285, 285, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 臨床的に間質性肺疾患と診断された犬25頭の回顧的検討
  川上 侑記; 中村 健介; 新坊 弦也; 山根 由久; 大菅 辰幸; 竹内 恭介; 木之下 怜平; 細谷 謙次; 永田 矩之; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 286, 286, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 犬尿中コルチゾール/クレアチニン比の基準値の確立とクッシング症候群の診断への応用
  永田 矩之; 澤村 飛龍; 五日市 大介; 森下 啓太郎; 細谷 謙次; 横山 望; 笹岡 一慶; 佐々木 東; 中村 健介; 池中 良徳; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 286, 286, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 院内湿潤環境に対する注意喚起 北大附属動物病院における環境サーベイランス結果
  笹岡 一慶; 新田 優里香; 佐藤 豊孝; 堀内 基広; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 287, 287, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 磁気共鳴スペクトロスコピーは犬の起源不明髄膜脳脊髄炎の予後指標となり得るか
  山口 敬央; 笹岡 一慶; 新坊 弦也; 山根 由久; 永田 矩之; 横山 望; 森下 啓太郎; 佐々木 東; 中村 健介; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 287, 287, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 造影CT検査後に非心原性肺水腫を呈した褐色細胞腫を疑う犬の1例
  吉田 紫乃; 田村 純; 永田 矩之; 大山 紀彦; 新坊 弦也; 池中 良徳; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 289, 289, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 麻酔中パルスオキシメーター測定異常から舌局所の灌流障害を疑った犬の1例
  田村 純; 湯本 優希; 大山 紀彦; 新坊 弦也; 滝口 満喜, 北海道獣医師会雑誌, 66, 8, 289, 289, Aug. 2022
  (公社)北海道獣医師会, Japanese
• 日本のペット動物における有機フッ素化合物の汚染実態
  野見山 桂; 佐藤 楓夏; 水川 葉月; 池中 良徳; 田上 瑠美; 横山 望; 滝口 満喜, 環境化学物質3学会合同大会要旨集, 2022年, 195, 196, Jun. 2022
  (一社)日本環境化学会・日本環境毒性学会・環境ホルモン学会, Japanese
• 犬の粘液腫様僧帽弁疾患に対する僧帽弁形成術後の左房リバースリモデリングの長期過程と予測因子の検討
  川元誠; 川元誠; 川元誠; 中村健介; 森拓也; 森拓也; 和田智樹; 和田智樹; 藤原彬; 藤原彬; 大橋美里; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 117th, 2022
• 二度目の肺血栓塞栓症により死亡した腎細胞癌の猫の1例
  湯本優希; 中村健介; 青島圭佑; 小林麻衣; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 117th, 2022
• 門脈静脈連結部での選択的結紮に術中超音波ガイドが有用であった右肝区域肝内門脈体循環短絡症の犬の1例
  松本創; 細谷謙次; 細谷謙次; 大脇稜; 大山紀彦; 竹内恭介; 木之下怜平; 金尚昊; 滝口満喜; 奥村正裕, 日本獣医麻酔外科学会学術集会抄録集(Web), 105th, 2022
• 「新型コロナ感染症(COVID-19)下での参加型臨床実習の実施 2020年度北海道大学動物医療センターにおける参加型臨床実習の実際
  滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSS, 2], Sep. 2021
  (公社)日本獣医学会, Japanese
• メタボロミクスおよび甲状腺ホルモン分析による有機ハロゲン汚染物質への曝露によって引き起こされたペット猫(Felis silvestris catus)の健康影響評価
  野見山 桂; 山本 恭央; 江口 哲史; 西川 博之; 水川 葉月; 横山 望; 市居 修; 滝口 満喜; 中山 翔太; 池中 良徳; 石塚 真由美; 国末 達也, 環境ホルモン学会研究発表会要旨集, 23回, 55, 55, Sep. 2021
  環境ホルモン学会(日本内分泌撹乱化学物質学会), Japanese
• 「新型コロナ感染症(COVID-19)下での参加型臨床実習の実施 2020年度北海道大学動物医療センターにおける参加型臨床実習の実際
  滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSS, 2], Sep. 2021
  (公社)日本獣医学会, Japanese
• 犬の僧帽弁疾患における2D Speckle Tracking心エコー図法により推定した左心房stiffnessと予後の関係
  大菅 辰幸; 黒田 杏子; 森田 智也; 中村 健介; 佐々木 東; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 8], Sep. 2021
  (公社)日本獣医学会, Japanese
• Comprehensive analysis of steroid profile in dogs with Cushing' syndrome and hepatocellular carcinoma(和訳中)
  Oo Thandar; 佐々木 東; 池中 良徳; 永田 矩之; 一瀬 貴大; Bin Teoh Yong; 川元 誠; 小島 一輝; 菅原 芽伊; 中山 翔太; 石塚 真由美; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 18], Sep. 2021
  (公社)日本獣医学会, English
• イヌの肝細胞腫瘍におけるDNAメチル化の網羅的解析
  淺利 友; 山崎 淳平; Oo Thander; 細谷 謙次; 金 尚昊; 木之下 怜平; 竹内 恭介; 賀川 由美子; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 30], Sep. 2021
  (公社)日本獣医学会, Japanese
• Identification of pathogenic Single Nucleotide Polymorphism in Inflammatory Colorectal Polyps of Miniature Dachshunds(和訳中)
  Bin Teoh Yong; 永田 矩之; 大田 寛; 佐々木 東; 中村 健介; 村上 正晃; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 32], Sep. 2021
  (公社)日本獣医学会, English
• 非再生性免疫介在性貧血の犬における脾臓摘出術前後の血清中蛋白の網羅的解析
  菅原 芽伊; 山崎 淳平; 森下 啓太郎; 金 尚昊; 細谷 謙次; 佐々木 東; 中村 健介; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 60], Sep. 2021
  (公社)日本獣医学会, Japanese
• Elucidation of the pathophysiology of hyperadrenocortical hyperfunction (Cushing’s syndrome) by simultaneous analysis of steroid hormones using LC-MS/MS
  一瀬貴大; 安田淑一; 佐々木東; 滝口満喜; 中山翔太; 石塚真由美; 池中良徳, JSBMS Letters, 46, Suppl., 75, 75, Aug. 2021
  (一社)日本医用マススペクトル学会, Japanese
• 重度肝線維化を伴う好酸球性肉芽腫を認めた好酸球増多症候群の若齢小型犬の1例
  篠原 正義; 田村 昌大; 金 尚昊; 賀川 由美子; 滝口 満喜, 北海道獣医師会雑誌, 65, 8, 262, 262, Aug. 2021
  (公社)北海道獣医師会, Japanese
• 長期生存している特発性被嚢性腹膜硬化症の猫の1例
  前田 晴香; 横山 望; 木之下 怜平; 大田 寛; 岡田 一喜; 賀川 由美子; 滝口 満喜, 北海道獣医師会雑誌, 65, 8, 263, 263, Aug. 2021
  (公社)北海道獣医師会, Japanese
• 左室壁肥厚と動的流出路閉塞を呈した犬の3例
  湯本 優希; 中村 健介; 志波 一恵; 寺澤 元子; 早坂 郁人; 滝口 満喜, 北海道獣医師会雑誌, 65, 8, 263, 263, Aug. 2021
  (公社)北海道獣医師会, Japanese
• 重度肝線維化を伴う好酸球性肉芽腫を認めた好酸球増多症候群の若齢小型犬の１例
  篠原正義; 田村昌大; 金 尚昊; 賀川由美子; 滝口満喜, 北海道獣医師会雑誌, 65, 8, 46, 46, Aug. 2021
• 非造影4D-MRAにより診断した鎖骨下動脈盗血症候群の犬の1症例
  新坊弦也; 越後良介; 越後良介; 滝口満喜, 北海道獣医師会雑誌, 65, 8, 268, 268, Aug. 2021
  (公社)北海道獣医師会, Japanese
• 超音波で生体の壁を超える 超音波と気泡を併用する膀胱内注入療法の犬における安全性検討
  佐々木 東; 青柳 貞一郎; 池中 良徳; 工藤 信樹; 中村 健介; 滝口 満喜, 超音波医学, 48, Suppl., S195, S195, Apr. 2021
  (公社)日本超音波医学会, Japanese
• 超音波で生体の壁を超える 超音波と気泡を併用する膀胱内注入療法の犬における安全性検討
  佐々木 東; 青柳 貞一郎; 池中 良徳; 工藤 信樹; 中村 健介; 滝口 満喜, 超音波医学, 48, Suppl., S195, S195, Apr. 2021
  (公社)日本超音波医学会, Japanese
• Speckle tracking法による右心房機能評価は慢性僧帽弁疾患犬において有用な指標となるか
  森田智也; 森田智也; 中村健介; 大菅辰幸; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 114th, 2021
• 動的左室流出路閉塞の犬4例
  湯本優希; 中村健介; 志波一恵; 寺澤元子; 幸野瑠美; 早坂郁人; 滝口満喜, 日本獣医循環器学会抄録(CD-ROM), 115th, 2021
• Analysis of variation of serum metabolites in cats by BDE209 long-term exposure test using Smart Metabolite Database
  野見山桂; 林多聞; 江口哲史; 水川葉月; 高口倖暉; 横山望; 市居修; 滝口満喜; 笹岡一慶; 中山翔太; 池中良徳; 石塚真由美; 国末達也, 環境化学討論会要旨集(CD-ROM), 29th, 2021
• メラトニンの経口投与が著効した両側性副腎腫大を伴う全身性脱毛症のフェレットの1例
  BIN Teoh Yong; 亀山健吾; 永田矩之; 佐々木東; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 65, 8, 265, 265, 2021
  (公社)北海道獣医師会, Japanese
• Safety aspects of ultrasound-assisted intravesical chemotherapy in dogs
  佐々木東; 青柳貞一郎; 池中良徳; 工藤信樹; 中村健介; 滝口満喜, 超音波医学 Supplement, 48, 2021
• 診療力が試される症例 Vet Board QUIZ 肝・胆道系・膵外分泌 若齢で腹水を認めたボーダー・コリー
  田村 昌大; 滝口 満喜; 中村 健介, Veterinary Board, 2, 12, 64, 69, Dec. 2020
  (株)エデュワードプレス, Japanese
• 最近の超音波による癌治療 その可能性と課題
  近藤 隆; 鈴木 亮; 佐々木 東; 滝口 満喜, Thermal Medicine, 36, 3, 75, 90, Sep. 2020
  (一社)日本ハイパーサーミア学会, Japanese
• 非再生性貧血のミニチュア・ダックスフンド11例に対する脾臓摘出術の治療成績
  菅原 芽伊; 森下 啓太郎; 今井 健友; 山崎 淳平; 佐々木 東; 大田 寛; 細谷 謙次; 滝口 満喜, SA Medicine, 22, 3, 74, 75, Jun. 2020
  (株)エデュワードプレス, Japanese
• イソフルラン麻酔下においてノルアドレナリンが全身循環に及ぼす影響
  小島 一輝; 石塚 友人; 佐藤 敬近; 滝口 満喜, 日本獣医麻酔外科学雑誌, 50, Suppl.2, 285, 285, Dec. 2019
  (一社)日本獣医麻酔外科学会, Japanese
• Two-dimensional shear wave elastographyによる非侵襲的な犬の肝線維化診断
  田村 昌大; 大田 寛; 大菅 辰幸; 新坊 弦也; 佐々木 東; 森下 啓太郎; 賀川 由美子; 滝口 満喜, 日本獣医麻酔外科学雑誌, 50, Suppl.2, 315, 315, Dec. 2019
  (一社)日本獣医麻酔外科学会, Japanese
• Two-dimensional shear wave elastographyによる非侵襲的な犬の肝線維化診断
  田村 昌大; 大田 寛; 大菅 辰幸; 新坊 弦也; 佐々木 東; 森下 啓太郎; 賀川 由美子; 滝口 満喜, 日本獣医麻酔外科学雑誌, 50, Suppl.2, 315, 315, Dec. 2019
  (一社)日本獣医麻酔外科学会, Japanese
• LC‐MS/MSを用いたイヌ尿中メタネフリンおよびノルメタネフリン測定
  井上裕美子; 佐々木東; 池中良徳; 一瀬貴大; 笹岡一慶; 大菅辰幸; 中山翔太; 森下啓太郎; 大田寛; 石塚真由美; 滝口満喜, 日本獣医学会学術集会講演要旨集, 162nd, 470, 20 Aug. 2019
  Japanese
• LC‐MS/MS法を用いたイヌ血清中ステロイドホルモンの分析法の確立と内分泌疾患モデルを用いた実測
  白尾大樹; 池中良徳; 一瀬貴大; 中山翔太; 佐々木東; 永田矩之; 水川葉月; 滝口満喜; 石塚真由美, 日本獣医学会学術集会講演要旨集, 162nd, 470, 20 Aug. 2019
  Japanese
• 【部位別の基本描出シリーズ 循環器編(3)心臓 各論 後天性疾患】超音波検査
  大菅 辰幸; 滝口 満喜, 伴侶動物画像診断, 4, 4, 28, 71, Aug. 2019
  (株)緑書房, Japanese
• LC-MS/MSを用いたイヌ尿中メタネフリンおよびノルメタネフリン測定
  井上 裕美子; 佐々木 東; 池中 良徳; 一瀬 貴大; 笹岡 一慶; 大菅 辰幸; 中山 翔太; 森下 啓太郎; 大田 寛; 石塚 真由美; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 162回, 470, 470, Aug. 2019
  (公社)日本獣医学会, Japanese
• LC-MS/MS法を用いたイヌ血清中ステロイドホルモンの分析法の確立と内分泌疾患モデルを用いた実測
  白尾 大樹; 池中 良徳; 一瀬 貴大; 中山 翔太; 佐々木 東; 永田 矩之; 水川 葉月; 滝口 満喜; 石塚 真由美, 日本獣医学会学術集会講演要旨集, 162回, 470, 470, Aug. 2019
  (公社)日本獣医学会, Japanese
• 北大附属動物病院における薬剤耐性モニタリング調査結果2018
  笹岡 一慶; 森下 啓太郎; 細谷 謙次; 滝口 満喜, 北海道獣医師会雑誌, 63, 8, 334, 334, Aug. 2019
  (公社)北海道獣医師会, Japanese
• ネコのアクロメガリー関連インスリン抵抗性糖尿病のコントロールを目的とした成長ホルモン受容体/Fc融合タンパク質の作製
  鈴木 理紗; 和泉 雄介; 前川 直也; 今内 覚; 高木 哲; 森下 啓太郎; 細谷 謙次; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 162回, 469, 469, Aug. 2019
  (公社)日本獣医学会, Japanese
• 部位別の基本描出シリーズ 循環器編 3)心臓 各論 後天性心疾患
  大菅辰幸; 滝口満喜, 伴侶動物画像診断, 4, 4, 28, 71, Aug. 2019, [Invited]
  Japanese, Introduction commerce magazine
• 詳細な病理学的解析に基づいて治療方針を決定した免疫複合体性糸球体腎炎の犬の1例
  新井田 篤; 大田 寛; 笹岡 一慶; 細谷 謙次; 市居 修; 賀川 由美子; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 63, 8, 336, 336, Aug. 2019
  (公社)北海道獣医師会, Japanese
• 過去3年間の輸血後副反応に関する回顧的研究
  細田 千尋; 森下 啓太郎; 山崎 淳平; 高田 健介; 細谷 謙次; 金 尚昊; 佐々木 東; 大田 寛; 滝口 満喜, 北海道獣医師会雑誌, 63, 8, 337, 337, Aug. 2019
  (公社)北海道獣医師会, Japanese
• 経皮的ドレナージで良好に経過した胃排出障害を伴う膵仮性嚢胞の犬の1例
  坂本 暁彦; 大田 寛; 杉浦 岳; 康井 翔平; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 63, 8, 341, 341, Aug. 2019
  (公社)北海道獣医師会, Japanese
• 肝皮症候群を疑い血漿アミノ酸解析を行った肝硬変の犬の1例
  上條 太貴; 大田 寛; 賀川 由美子; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 63, 8, 341, 341, Aug. 2019
  (公社)北海道獣医師会, Japanese
• 犬の先天性肝外門脈体循環シャントの術後合併症率と危険因子に関する回顧的研究
  鈴木 理紗; 細谷 謙次; 金 尚昊; 森下 啓太郎; 大田 寛; 滝口 満喜; 奥村 正裕, 北海道獣医師会雑誌, 63, 8, 349, 349, Aug. 2019
  (公社)北海道獣医師会, Japanese
• 犬の僧帽弁疾患における2D Speckle Tracking心エコー図法を用いた左心房stiffnessの推定
  黒田 杏子; 大菅 辰幸; 笹岡 一慶; 森下 啓太郎; 佐々木 東; 大田 寛; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 162回, 464, 464, Aug. 2019
  (公社)日本獣医学会, Japanese
• 猫の新規炎症マーカー、ロイシンリッチα2糖蛋白質(LRG)の測定系構築の試み
  坂本 暁彦; 大田 寛; 好井 健太朗; 矢吹 悠久美; 笹岡 一慶; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 162回, 475, 475, Aug. 2019
  (公社)日本獣医学会, Japanese
• PCBs曝露によるネコ肝トランスクリプトームへの影響
  水川葉月; NGYUEN Hoa Thanh; 岩田久人; 野見山桂; 池中良徳; 中山翔太; 横山望; 市居修; 滝口満喜; KHIDKHAN Kraisiri; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 28th, ROMBUNNO.1C‐08, 11 Jun. 2019
  Japanese
• デカブロモジフェニルエーテル(BDE209)の長期曝露がイエネコへ及ぼす影響
  田中啓介; 野見山桂; 水川葉月; 水川葉月; 高口倖暉; 田上瑠美; 横山望; 市居修; 滝口満喜; 笹岡一慶; 中山翔太; 池中良徳; 石塚真由美; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 28th, ROMBUNNO.1C‐09, 11 Jun. 2019
  Japanese
• 【部位別の基本描出シリーズ 循環器編(2)心臓 各論 先天性疾患】超音波検査
  大菅 辰幸; 滝口 満喜, 伴侶動物画像診断, 4, 3, 24, 66, Jun. 2019
  (株)緑書房, Japanese
• 部位別の基本描出シリーズ 循環器編 2)心臓 各論 先天性心疾患
  大菅辰幸; 滝口満喜, 伴侶動物画像診断, 4, 3, 24, 66, Jun. 2019, [Invited]
  Japanese
• 慢性僧帽弁疾患犬において心室間流入時間差は有用な心機能評価指標である
  森田 智也; 中村 健介; 大菅 辰幸; 滝口 満喜, 日本獣医麻酔外科学雑誌, 50, Suppl.1, 322, 322, Jun. 2019
  (一社)日本獣医麻酔外科学会, Japanese
• 閉塞性黄疸はShear Wave Elastographyによる肝硬度測定に影響を与える
  田村 昌大; 大田 寛; 新坊 弦也; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 日本獣医麻酔外科学雑誌, 50, Suppl.1, 333, 333, Jun. 2019
  (一社)日本獣医麻酔外科学会, Japanese
• 大学動物病院の活動の現状とさらなる発展を目指して 北海道大学動物医療センターの取組みと課題
  滝口 満喜, 日本獣医師会雑誌, 72, 5, 240, 242, May 2019
  (公社)日本獣医師会, Japanese
• 蚕白喪失性腸症の犬40例からの腸リンパ管拡張症の診断と治療に関する考察
  永田 矩之; 大田 寛; 横山 望; 佐々木 東; 大菅 辰幸; 森下 啓太郎; 滝口 満喜, 日本獣医師会雑誌, 72, 4, 234, 235, Apr. 2019
  (公社)日本獣医師会, Japanese
• 【部位別の基本描出シリーズ 循環器編(1) 心臓 総論】超音波検査
  大菅 辰幸; 滝口 満喜, 伴侶動物画像診断, 4, 2, 27, 68, Apr. 2019
  (株)緑書房, Japanese
• 部位別の基本描出シリーズ 循環器編 1)心臓 総論 超音波検査
  大菅辰幸; 滝口満喜, 伴侶動物画像診断, 4, 2, 27, 68, Apr. 2019, [Invited]
  Japanese
• 蚕白喪失性腸症の犬40例からの腸リンパ管拡張症の診断と治療に関する考察
  永田 矩之; 大田 寛; 横山 望; 佐々木 東; 大菅 辰幸; 森下 啓太郎; 滝口 満喜, 日本獣医師会雑誌, 72, 4, 234, 235, Apr. 2019
  (公社)日本獣医師会, Japanese
• 低強度パルス超音波照射による毛髪細胞刺激
  佐々木 東; 工藤 信樹; 大田 寛; 滝口 満喜, 超音波医学, 46, Suppl., S579, S579, Apr. 2019
  (公社)日本超音波医学会, Japanese
• 蛋白喪失性腸症の犬40例からの腸リンパ管拡張症の診断と治療に関する考察
  永田矩之; 大田寛; 横山望; 佐々木東; 大菅辰幸; 森下啓太郎; 滝口満喜, 日本獣医師会獣医学術学会年次大会講演要旨集, 2018, 2019
• 慢性僧帽弁疾患犬において心室間流入時間差は有用な心機能評価指標である
  森田智也; 中村健介; 大菅辰幸; 大菅辰幸; 滝口満喜, 日本獣医麻酔外科学雑誌(Web), 50, Supplement 1, 2019
• ピモベンダンは慢性塞栓性肺高血圧症において圧を上げずに拍出量を上げる
  森田 智也; 中村 健介; 大菅 辰幸; 滝口 満喜, 日本獣医麻酔外科学雑誌, 49, Suppl.2, 242, 242, Dec. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の僧帽弁疾患における心エコー図法により推定した左心室-動脈カップリングとステージの関係
  大菅 辰幸; 森田 智也; 佐々木 東; 森下 啓太郎; 大田 寛; 滝口 満喜, 日本獣医麻酔外科学雑誌, 49, Suppl.2, 249, 249, Dec. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• Shear Wave Elastographyによる犬の肝硬度評価の試み
  田村 昌大; 森下 啓太郎; 新坊 弦也; 佐々木 東; 大田 寛; 滝口 満喜, 日本獣医画像診断学会抄録, 64回, 13, 13, Nov. 2018
  (一社)日本獣医画像診断学会, Japanese
• やっぱり難しい膵炎診断 症状、膵リパーゼ、画像をどう評価するか? 膵炎の画像診断を中心に
  滝口 満喜, 動物臨床医学会年次大会プロシーディング, 39回, 1, 77, 77, Nov. 2018
  動物臨床医学会, Japanese
• 超音波造影を含めた超音波検査の将来
  滝口 満喜, 日本獣医画像診断学会抄録, 64回, 9, 9, Nov. 2018
  (一社)日本獣医画像診断学会, Japanese
• Shear Wave Elastographyによる犬の肝硬度評価の試み
  田村 昌大; 森下 啓太郎; 新坊 弦也; 佐々木 東; 大田 寛; 滝口 満喜, 日本獣医画像診断学会抄録, 64回, 13, 13, Nov. 2018
  日本獣医画像診断学会, Japanese
• 犬の非再生性免疫介在性貧血の臨床病理所見と治療反応に関する回顧的研究
  岩永優斗; 森下啓太郎; 細谷謙次; 大菅辰幸; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 62, 8, 334, 24 Aug. 2018
  Japanese
• シスプラチン膀胱内ソノポレーションの前臨床試験的検討
  佐々木東; 中山翔太; 大田寛; 大菅辰幸; 森下啓太郎; 水川葉月; 池中良徳; 石塚真由美; 滝口満喜, 日本獣医学会学術集会講演要旨集, 161st, 429, 21 Aug. 2018
  Japanese
• 犬の非再生性免疫介在性貧血の臨床病理所見と治療反応に関する回顧的研究
  岩永 優斗; 森下 啓太郎; 細谷 謙次; 大菅 辰幸; 佐々木 東; 大田 寛; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 334, 334, Aug. 2018
  (公社)北海道獣医師会, Japanese
• シスプラチン膀胱内ソノポレーションの前臨床試験的検討
  佐々木 東; 中山 翔太; 大田 寛; 大菅 辰幸; 森下 啓太郎; 水川 葉月; 池中 良徳; 石塚 真由美; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 161回, 429, 429, Aug. 2018
  (公社)日本獣医学会, Japanese
• 術前からダイナミックな血圧変動を呈した褐色細胞腫を良好に麻酔管理した犬の1例
  小島 一輝; 石塚 友人; 金 尚昊; 竹内 恭介; 細谷 謙次; 滝口 満喜; 奥村 正裕, 北海道獣医師会雑誌, 62, 8, 336, 336, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 蛋白喪失性腸症の犬33例からの腸リンパ管拡張症の診断と治療に関する考察
  永田 矩之; 大田 寛; 横山 望; ニサ・ホイルン; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 334, 334, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 神経筋接合部検査としてアイスパックテストを試みた重症筋無力症の犬の1例
  山田 有紗; 笹岡 一慶; 大田 寛; 立花 由莉加; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 335, 335, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 犬の粘液腫様変性性僧帽弁疾患における心エコー図法による左心室-大動脈カップリングの推定
  大菅 辰幸; 佐々木 東; 森下 啓太郎; 大田 寛; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 335, 335, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 神経症状を主訴とした悪性中皮腫の猫の1例
  大村 侑己; 大田 寛; 小林 篤史; 笹岡 一慶; 道下 明日香; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 340, 340, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 経頭蓋超音波ドプラ法により局所的な脳循環動態を評価し得た右中大脳動脈梗塞の犬の1例
  笹岡 一慶; 大田 寛; 石塚 友人; 大菅 辰幸; 立花 由莉加; 森下 啓太郎; 佐々木 東; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 341, 341, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 鼻腔内真菌感染症に対しクロトリマゾール灌流および注入療法を実施した犬の1例
  大谷 豪; 大田 寛; 岩木 有香; 細谷 謙次; 新坊 弦也; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 62, 8, 344, 344, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 健康な犬における二次元スペックルトラッキング心エコーによる左心室長軸方向ストレイン解析に与える心臓容積負荷の影響(Effect of cardiac volume loading on left atrial longitudinal strain analysis derived from two-dimensional Speckle Tracking Echocardiography in healthy dogs)
  Dermlim Angkhana; 中村 健介; 森田 智也; 大菅 辰幸; Nisa Khoirun; Leela-arporn Rommaneeya; 笹岡 一慶; 永田 矩之; 田村 昌大; 佐々木 東; 大田 寛; 森下 啓太郎; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 161回, 435, 435, Aug. 2018
  (公社)日本獣医学会, English
• 蛋白喪失性腸症の犬33例からの腸リンパ管拡張症の診断と治療に関する考察
  永田矩之; 大田寛; 横山望; ニサ ホイルン; 佐々木東; 森下啓太郎; 滝口満喜, 北海道獣医師会雑誌, 62, 8, 334, 334, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 神経筋接合部検査としてアイスパックテストを試みた重症筋無力症の犬の1例
  山田有紗; 笹岡一慶; 大田寛; 立花由莉加; 大菅辰幸; 佐々木東; 森下啓太郎; 滝口満喜, 北海道獣医師会雑誌, 62, 8, 335, 335, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 経頭蓋超音波ドプラ法により局所的な脳循環動態を評価し得た右中大脳動脈梗塞の犬の1例
  笹岡一慶; 大田寛; 石塚友人; 大菅辰幸; 立花由莉加; 森下啓太郎; 佐々木東; 滝口満喜, 北海道獣医師会雑誌, 62, 8, 341, 341, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 神経症状を主訴とした悪性中皮腫の猫の1例
  大村侑己; 大田寛; 小林篤史; 笹岡一慶; 道下明日香; 大菅辰幸; 佐々木東; 森下啓太郎; 滝口満喜, 北海道獣医師会雑誌, 62, 8, 340, 340, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 犬の粘液腫様変性性僧帽弁疾患における心エコー図法による左心室‐大動脈カップリングの推定
  大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 62, 8, 335, 335, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 鼻腔内真菌感染症に対しクロトリマゾール潅流および注入療法を実施した犬の1例
  大谷豪; 大田寛; 岩木有香; 細谷謙次; 新坊弦也; 佐々木東; 森下啓太郎; 滝口満喜, 北海道獣医師会雑誌, 62, 8, 344, 344, Aug. 2018
  (公社)北海道獣医師会, Japanese
• 術前からダイナミックな血圧変動を呈した褐色細胞腫を良好に麻酔管理した犬の1例
  小島 一輝; 石塚 友人; 金 尚昊; 竹内 恭介; 細谷 謙次; 滝口 満喜; 奥村 正裕, 北海道獣医師会雑誌, 62, 8, 336, 336, Aug. 2018
  (公社)北海道獣医師会, Japanese
• Shear Wave Elastography(SWE)を用いた犬の肝硬度測定
  田村 昌大; 大田 寛; 佐々木 東; 賀川 由美子; 滝口 満喜, 獣医畜産新報, 71, 7, 496, 497, Jul. 2018
  文永堂出版(株), Japanese
• Shear Wave Elastography(SWE)を用いた犬の肝硬度測定
  田村昌大; 大田寛; 佐々木東; 賀川由美子; 滝口満喜, 獣医畜産新報, 71, 7, 496, 497, Jul. 2018
  文永堂出版(株), Japanese
• 慢性僧帽弁疾患犬において右室strainによる右心室機能評価は優れた予後指標となり得るか
  森田 智也; 中村 健介; 大菅 辰幸; 森下 啓太郎; 佐々木 東; 大田 寛; 滝口 満喜, SA Medicine, 20, 3, 68, 70, Jun. 2018
  (株)エデュワードプレス, Japanese
• ポリ塩化ビフェニル曝露によるイヌ・ネコの甲状腺ホルモン恒常性への影響
  高口 倖暉; 野見山 桂; 西川 博之; 水川 葉月; 田上 瑠美; 草木 桃子; 横山 望; 市居 修; 滝口 満喜; 中山 翔太; 池中 良徳; 石塚 真由美; 岩田 久人; 国末 達也; 田辺 信介, The Journal of Toxicological Sciences, 43, Suppl., S245, S245, Jun. 2018
  (一社)日本毒性学会, Japanese
• 肺塞栓症において認められる心室中隔の奇異性運動は左右心室間同期障害によって生じる
  中村健介; 中村健介; 森田智也; 大菅辰幸; 池田正悟; DERMLIM Angkhana; 滝口満喜, 日本獣医麻酔外科学雑誌, 49, Suppl.1, 301, 301, Jun. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• 慢性僧帽弁疾患犬において右室strainによる右心室機能評価は優れた予後指標となり得るか
  森田智也; 中村健介; 大菅辰幸; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, SA Medicine, 20, 3, 68‐70, 01 Jun. 2018
  Japanese
• 有機ハロゲン化合物曝露によるネコの生化学マーカーの変化および甲状腺機能に及ぼす影響評価
  水川葉月; KHIDKHAN Kraisiri; 高口倖暉; 横山望; 市居修; 滝口満喜; 笹岡一慶; 池中良徳; 池中良徳; 中山翔太; TSEND‐AYUSH Sainnoxoi; 野見山桂; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 27th, ROMBUNNO.2A‐10, 11 May 2018
  Japanese
• ネコのin vivo BDE209長期曝露試験による体内動態の解明および甲状腺ホルモンへの影響評価
  田中啓介; 野見山桂; 水川葉月; 高口倖暉; 田上瑠美; 横山望; 市居修; 滝口満喜; 笹岡一慶; 中山翔太; 池中良徳; 石塚真由美; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 27th, ROMBUNNO.1A‐03(PA‐13), 11 May 2018
  Japanese
• 犬の蛋白漏出性腸症の予後因子解析 超音波検査所見の有用性の検討
  大田 寛; 賀川 由美子; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 日本獣医師会雑誌, 71, 4, 206, 207, Apr. 2018
  (公社)日本獣医師会, Japanese
• 【部位別の基本描出シリーズ 内分泌器官編 甲状腺・上皮小体】超音波検査
  滝口 満喜, 伴侶動物画像診断, 3, 2, 54, 62, Apr. 2018
  (株)緑書房, Japanese
• ロジカルに修得する超音波検査(第1回) 総論 超音波診断装置とその調整
  滝口 満喜, 伴侶動物画像診断, 3, 2, 98, 101, Apr. 2018
  (株)緑書房, Japanese
• 犬の蛋白漏出性腸症の予後因子解析 超音波検査所見の有用性の検討
  大田 寛; 賀川 由美子; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 日本獣医師会雑誌, 71, 4, 206, 207, Apr. 2018
  (公社)日本獣医師会, Japanese
• 低強度パルス超音波による神経軸索の伸展制御の試み
  佐々木東; 工藤信樹; 大田寛; 滝口満喜, 超音波医学, 45, Suppl., S575, S575, Apr. 2018
  (公社)日本超音波医学会, Japanese
• 血清中メタボローム解析によるペットネコ(Felis catus)の有機ハロゲン化合物毒性影響評価
  野見山桂; 江口哲史; 西川博之; 水川葉月; 山本恭央; 滝口満喜; 中山翔太; 池中良徳; 石塚真由美; 田辺信介, 環境化学討論会要旨集(CD-ROM), 27th, 2018
• JCVIM 2018 臨床研究アワード 慢性僧帽弁疾患犬において右室strainによる右心室機能評価は優れた予後指標となり得るか (第14回日本獣医内科学アカデミー学術大会(JCVIM 2018) 〈臨床研究アワード〉日本獣医内科学アカデミー/インターズー学術奨励賞受賞作品ダイジェスト版)
  森田 智也; 中村 健介; 大菅 辰幸; 森下 啓太郎; 佐々木 東; 大田 寛; 滝口 満喜, SA medicine : Journal of small animal medicine, 24, 3, 68, 70, 2018
  インターズー, Japanese
• Effects of PCB-exposure on thyroid hormone homeostasis in dog (canis lupus familiaris) and cat (felis catus)
  TAKAGUCHI Kohki; NAKAYAMA Shouta; IKENAKA Yoshinori; ISHIZUKA Mayumi; IWATA Hisato; KUNISUE Tatsuya; TANABE Shinsuke; NOMIYAMA Kei; NISHIKAWA Hiroyuki; MIZUKAWA Hazuki; TANOUE Rumi; KUSAKI Momoko; YOKOYAMA Nozomu; ICHII Osamu; TAKIGUCHI Mitsuyoshi, Annual Meeting of the Japanese Society of Toxicology, 45, 0, P, 72, 2018
  <p>[in Japanese] </p>, The Japanese Society of Toxicology, Japanese
• 内分泌性脱毛症の2例
  島崎 博美; 飯田 大起; 佐々木 東; 滝口 満喜; 藤井 義晴, (一社)比較統合医療学会学術大会・日本補完代替医療学会学術集会プログラム・抄録集, 60回・20回, 57, 58, Dec. 2017
  (一社)比較統合医療学会・日本補完代替医療学会, Japanese
• ポリ塩化ビフェニル(PCBs)がネコの甲状腺機能に及ぼす影響
  水川 葉月; 高居 名菜; 野見山 桂; 高口 倖暉; 横山 望; 市居 修; 滝口 満喜; 池中 良徳; 中山 翔太; 石塚 真由美, 環境ホルモン学会研究発表会要旨集, 20回, 78, 78, Dec. 2017
  環境ホルモン学会(日本内分泌撹乱化学物質学会), Japanese
• ポリ塩化ビフェニル(PCBs)がネコの甲状腺機能に及ぼす影響
  水川葉月; 高居名菜; 野見山桂; 高口倖暉; 横山望; 市居修; 滝口満喜; 池中良徳; 池中良徳; 中山翔太; 石塚真由美, 日本内分泌かく乱化学物質学会研究発表会要旨集, 20th, 78, 78, Dec. 2017
  環境ホルモン学会(日本内分泌撹乱化学物質学会), Japanese
• 消化管エコーのノウハウ
  滝口 満喜, 動物臨床医学会年次大会プロシーディング, 38回, 1, 91, 91, Nov. 2017
  動物臨床医学会, Japanese
• すぐに診療で活かせる超音波画像診断の臨床
  滝口 満喜, 動物臨床医学会年次大会プロシーディング, 38回, 3, 205, 205, Nov. 2017
  動物臨床医学会, Japanese
• 生前診断に苦慮した化膿性腸炎の猫の1例
  森下 啓太郎; 中島 彩乃; 賀川 由美子; 華園 究; 佐々木 東; 大田 寛; 滝口 満喜, 日本獣医画像診断学会抄録, 62回, 6, 6, Sep. 2017
  (一社)日本獣医画像診断学会, Japanese
• 生前診断に苦慮した化膿性腸炎の猫の1例
  森下啓太郎; 中島彩乃; 賀川由美子; 華園究; 佐々木東; 大田寛; 滝口満喜, 日本獣医画像診断学会抄録(Web), 62nd, 6 (WEB ONLY), Sep. 2017
  Japanese
• 超音波と膀胱内注入療法を組み合わせた膀胱癌治療法の開発
  佐々木東; 中山翔太; 森下啓太郎; 中村健介; 大田寛; 水川葉月; 池中良徳; 石塚真由美; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160th, 461, 30 Aug. 2017
  Japanese
• 犬の右肝区域におけるCT画像解析を用いた静脈血管走行の多様性調査
  西野綾乃; 華園究; 石塚友人; 星野有希; 森下啓太郎; 滝口満喜; 高木哲, 日本獣医学会学術集会講演要旨集, 160th, 455, 30 Aug. 2017
  Japanese
• 犬の右肝区域におけるCT画像解析を用いた静脈血管走行の多様性調査
  西野 綾乃; 華園 究; 石塚 友人; 星野 有希; 森下 啓太郎; 滝口 満喜; 高木 哲, 日本獣医学会学術集会講演要旨集, 160回, 455, 455, Aug. 2017
  (公社)日本獣医学会, Japanese
• 超音波と膀胱内注入療法を組み合わせた膀胱癌治療法の開発
  佐々木 東; 中山 翔太; 森下 啓太郎; 中村 健介; 大田 寛; 水川 葉月; 池中 良徳; 石塚 真由美; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 160回, 461, 461, Aug. 2017
  (公社)日本獣医学会, Japanese
• 造影超音波検査により計測する肺通過時間は肺動脈塞栓モデル犬において短縮する
  中村 健介; 森田 智也; 大菅 辰幸; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 160回, 465, 465, Aug. 2017
  (公社)日本獣医学会, Japanese
• 副腎偶発腫から診断した犬のサブクリニカルクッシング症候群の臨床的特徴
  永田矩之; 大田寛; 佐々木東; 森下啓太郎; 中村康介; 中村康介; 細谷謙次; 高木哲; 滝口満喜, 北海道獣医師会雑誌, 61, 8, 326, 326, Aug. 2017
  (公社)北海道獣医師会, Japanese
• 組織球性大腸炎のフレンチ・ブルドッグの1例
  立花由莉加; 大田寛; 賀川由美子; 佐々木東; 森下啓太郎; 滝口満喜, 北海道獣医師会雑誌, 61, 8, 348, 348, Aug. 2017
  (公社)北海道獣医師会, Japanese
• 後天性門脈体循環シャントを合併した先天性肝線維症の猫の1例
  田村昌大; 森下啓太郎; 高木哲; 早川小百合; 賀川由美子; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 61, 8, 345, 345, Aug. 2017
  (公社)北海道獣医師会, Japanese
• 市販フードの給餌により栄養性二次性上皮小体機能亢進症を生じた犬の2例
  道下明日香; 森下啓太郎; 橋場香野; 田村昌大; 金尚昊; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 61, 8, 327, 327, Aug. 2017
  (公社)北海道獣医師会, Japanese
• 犬の蛋白漏出性腸症の予後因子解析:超音波検査所見の有用性の検討
  大田寛; 賀川由美子; 佐々木東; 森下啓太郎; 滝口満喜, 北海道獣医師会雑誌, 61, 8, 347, 347, Aug. 2017
  (公社)北海道獣医師会, Japanese
• 犬の原発性免疫介在性血小板減少症(pIMT)再発症例の回顧的研究
  高村大地; 森下啓太郎; 中島彩乃; 竹内恭介; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 61, 8, 332, 332, Aug. 2017
  (公社)北海道獣医師会, Japanese
• 犬慢性腸症の十二指腸粘膜におけるロイシンリッチα2糖蛋白の発現とその臨床的意義
  奥朋哉; 大田寛; 田村昌大; 永田矩之; 笹岡一慶; LEELA‐ARPORN Rommaneeya; DERMLIM Angkhana; NISA Khoirun; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160回, 477, 477, Aug. 2017
  (公社)日本獣医学会, Japanese
• 全エクソームシーケンスを用いたミニチュア・ダックスフントの炎症性結直腸ポリープ感受性遺伝子の探索
  永田矩之; 大田寛; 横山望; 田村昌大; 笹岡一慶; LEELA‐ARPORN Rommaneeya; DERMLIM Angkhana; NISA Khoirun; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160回, 477, 477, Aug. 2017
  (公社)日本獣医学会, Japanese
• 犬虚血性脳血管障害における脳底動脈血流の経頭蓋超音波ドプラ法による評価
  笹岡一慶; 大田寛; 田村昌大; 永田矩之; LEELA‐ARPORN Rommaneeya; DERMLIM Angkhana; KHOIRUN Nisa; 森田智也; 佐々木東; 森下啓太郎; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160回, 465, 465, Aug. 2017
  (公社)日本獣医学会, Japanese
• 慢性肺動脈塞栓性肺高血圧症モデル犬を用いたピモベンダンの作用に関する検討
  森田智也; 中村健介; 大菅辰幸; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 日本獣医学会学術集会講演要旨集, 160回, 465, 465, Aug. 2017
  (公社)日本獣医学会, Japanese
• 慢性腸疾患の犬における十二指腸の定量的造影超音波法(Quantitative contrast-enhanced ultrasonography of duodenum in dogs with chronic enteropathy)
  Khoirun Nisa; 大田 寛; 横山 望; 田村 昌大; 永田 矩之; 笹岡 一慶; Dermlim Angkhana; Leela-Arporn Rommaneeya; 森田 智也; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 160回, 477, 477, Aug. 2017
  (公社)日本獣医学会, English
• ソノポレーションを用いた膀胱内注入療法の開発 (特集 進化する超音波治療技術の最前線(1))
  佐々木 東; 工藤 信樹; 中山 翔太; 滝口 満喜, 超音波techno, 29, 4, 27, 30, Jul. 2017
  日本工業出版, Japanese
• ポリ塩化ビフェニル(PCBs)暴露によるネコ甲状腺機能への影響評価~甲状腺ホルモンの脱ヨード化と代謝能の解析~
  水川葉月; 高居名菜; 横山望; 市居修; 滝口満喜; 野見山桂; 高口倖暉; 西川博之; 池中良徳; 池中良徳; 中山翔太; SAINNOXOI Tsend‐ayush; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐01, 05 Jun. 2017
  Japanese
• PCBs曝露がイヌ・ネコの甲状腺ホルモン恒常性へ及ぼす影響
  高口倖暉; 野見山桂; 西川博之; 水川葉月; 田上瑠美; 芳之内結加; 横山望; 市居修; 滝口満喜; 中山翔太; 池中良徳; 石塚真由美; 岩田久人; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐02, 05 Jun. 2017
  Japanese
• イエネコのin vivo長期曝露試験:デカブロモジフェニルエーテル(BDE209)は甲状腺機能亢進症を引き起こすのか?
  水川葉月; 野見山桂; 池中良徳; 池中良徳; 中山翔太; SAENGTIENCHAI Aksorn; SAINNOXOI Tsendayush; 横山望; 笹岡一慶; 市居修; KHIDKHAN Kraisiri; 高居名菜; 滝口満喜; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐03, 05 Jun. 2017
  Japanese
• イエネコのin vivo長期曝露試験:デカブロモジフェニルエーテル(BDE209)の生体内変化
  田中啓介; 野見山桂; 水川葉月; 高口倖暉; 田上瑠美; SAENGTIENCHAI Aksorn; SAINNOXOI Tsend‐ayush; 横山望; 市居修; 滝口満喜; 中山翔太; 池中良徳; 石塚真由美; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐04, 05 Jun. 2017
  Japanese
• 血清中メタボローム解析によるイエネコのPCBs毒性影響評価
  野見山桂; 西川博之; 水川葉月; 高口倖暉; SAINNOXOI Tsend‐ayush; 横山望; 市居修; 滝口満喜; 石塚真由美; 池中良徳; 中山翔太; 江口哲史; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐05, 05 Jun. 2017
  Japanese
• ネコの甲状腺機能に対するポリ塩化ビフェニル(PCBs)の影響評価 甲状腺ホルモンの脱ヨード化と代謝能の解析
  水川 葉月; 高居 名菜; 横山 望; 市居 修; 滝口 満喜; 野見山 桂; 高口 倖暉; 西川 博之; 池中 良徳; 中山 翔太; Sainnoxoi Tsend-ayush; 田辺 信介; 石塚 真由美, The Journal of Toxicological Sciences, 42, Suppl., S236, S236, Jun. 2017
  (一社)日本毒性学会, Japanese
• 正常犬ならびに慢性僧帽弁疾患犬におけるピモベンダン投与による左心房機能の変化
  三木伸悟; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 48, Suppl.1, 348, 348, Jun. 2017
  (一社)日本獣医麻酔外科学会, Japanese
• 急性圧負荷が右室にもたらす影響:心エコー図検査は何を反映しているか
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 48, Suppl.1, 336, 336, Jun. 2017
  (一社)日本獣医麻酔外科学会, Japanese
• 先天性門脈体循環シャントに伴う肝肺症候群および門脈肺高血圧症を疑った1例
  道下明日香; 中村健介; 森下啓太郎; 佐々木東; 大田寛; 細谷謙次; 賀川由美子; 滝口満喜, 日本獣医麻酔外科学雑誌, 48, Suppl.1, 220, 220, Jun. 2017
  (一社)日本獣医麻酔外科学会, Japanese
• ネコの甲状腺機能に対するポリ塩化ビフェニル(PCBs)の影響評価~甲状腺ホルモンの脱ヨード化と代謝能の解析~
  水川葉月; 高居名菜; 横山望; 市居修; 滝口満喜; 野見山桂; 高口倖暉; 西川博之; 池中良徳; 池中良徳; 中山翔太; SAINNOXOI Tsend‐Ayush; 田辺信介; 石塚真由美, Journal of Toxicological Sciences, 42, Supplement, S236, Jun. 2017
  有機ハロゲン化合物の一種であるポリ塩化ビフェニル（PCBs）は甲状腺ホルモン（THs）の恒常性かく乱が懸念されており、実際、ヒトやげっ歯類においてはPCBs暴露により血中THs濃度の低下が認められている。ヒトの身近な動物であるネコは餌などを介してPCBsに暴露していると懸念され、THs恒常性に何らかの悪影響をもたらすことが予想される。本研究では、ネコの甲状腺機能に対するPCBsの影響を解析するため、in vivo投与試験したネコを用いて甲状腺機能への影響を解析した。血中のTHs濃度（総T4、総T3、遊離T4、遊離T3、リバースT3）を解析した結果、対照群と暴露群の間に有意な差は見られなかった。また、THsの恒常性に関与する遺伝子（甲状腺ホルモン受容体αおよびβ、脱ヨード酵素type1）の肝臓中発現量やTHs代謝に関わる酵素であるグルクロン酸抱合酵素（UGT1A1）の活性を調べたところ、対照群と暴露群の間に有意な差は見られなかった。
  本実験結果では、ネコにおいてPCBs短期暴露によるTHsへの影響が認められなかったが、日本のペットネコにおけるモニタリング調査では、血中のTHs濃度とPCBs濃度との間に負の相関が見られており、有機ハロゲン化合物によるTHsかく乱が懸念されている。また、本研究試料のメタボローム解析結果では酸化ストレスの発現、抗酸化物質、電子伝達物質の生産阻害などが示唆されており、PCBsや水酸化代謝物による内分泌撹乱作用が心配される。THsの恒常性は非常に複雑な機構のもと維持されていることが知られており、負のフィードバック機構に加え、THsの活性化や代謝、排泄の調整にも多くの遺伝子やトランスポーター、酵素など様々な要因が関わっていることから、ネコは長期的かつ慢性的に化学物質に暴露されることで、甲状腺機能などへの悪影響が現れると考えられ、内分泌撹乱作用の作用機序について解析を進めることが重要である。, The Japanese Society of Toxicology, Japanese
• Thyroid hormone effects of polychlorinated biphenyls (PCBs) in cats-Analysis of deiodination and metabolism-
  MIZUKAWA Hazuki; NAKAYAMA Shouta M.M; SAINNOXOI Tsend-ayush; TANABE Shinsuke; ISHIZUKA Mayumi; TAKAI Nana; YOKOYAMA Nozomu; ICHII Osamu; TAKIGUCHI Mitsuyoshi; NOMIYAMA Kei; TAKAGUCHI Kohki; NISHIKAWA Hiroyuki; IKENAKA Yoshinori, Annual Meeting of the Japanese Society of Toxicology, 44, 0, P, 55, Jun. 2017
  有機ハロゲン化合物の一種であるポリ塩化ビフェニル（PCBs）は甲状腺ホルモン（THs）の恒常性かく乱が懸念されており、実際、ヒトやげっ歯類においてはPCBs暴露により血中THs濃度の低下が認められている。ヒトの身近な動物であるネコは餌などを介してPCBsに暴露していると懸念され、THs恒常性に何らかの悪影響をもたらすことが予想される。本研究では、ネコの甲状腺機能に対するPCBsの影響を解析するため、<i>in vivo</i>投与試験したネコを用いて甲状腺機能への影響を解析した。血中のTHs濃度（総T4、総T3、遊離T4、遊離T3、リバースT3）を解析した結果、対照群と暴露群の間に有意な差は見られなかった。また、THsの恒常性に関与する遺伝子（甲状腺ホルモン受容体αおよびβ、脱ヨード酵素type1）の肝臓中発現量やTHs代謝に関わる酵素であるグルクロン酸抱合酵素（UGT1A1）の活性を調べたところ、対照群と暴露群の間に有意な差は見られなかった。<br>本実験結果では、ネコにおいてPCBs短期暴露によるTHsへの影響が認められなかったが、日本のペットネコにおけるモニタリング調査では、血中のTHs濃度とPCBs濃度との間に負の相関が見られており、有機ハロゲン化合物によるTHsかく乱が懸念されている。また、本研究試料のメタボローム解析結果では酸化ストレスの発現、抗酸化物質、電子伝達物質の生産阻害などが示唆されており、PCBsや水酸化代謝物による内分泌撹乱作用が心配される。THsの恒常性は非常に複雑な機構のもと維持されていることが知られており、負のフィードバック機構に加え、THsの活性化や代謝、排泄の調整にも多くの遺伝子やトランスポーター、酵素など様々な要因が関わっていることから、ネコは長期的かつ慢性的に化学物質に暴露されることで、甲状腺機能などへの悪影響が現れると考えられ、内分泌撹乱作用の作用機序について解析を進めることが重要である。, 日本毒性学会, Japanese
• 低強度パルス超音波による毛包構成細胞への刺激
  佐々木東; 工藤信樹; 中村健介; 森下啓太郎; 大田寛; 滝口満喜, 再生医療, 16, 348, 01 Feb. 2017
  Japanese
• 毛包を構成する細胞に対する低強度超音波刺激
  佐々木東; 工藤信樹; 森下啓太郎; 大田寛; 滝口満喜, 日本超音波医学会光超音波画像研究会プログラム・抄録集, 2017, 2nd, 66‐67, 2017
  Japanese
• 犬の甲状腺機能低下症を誤診しないように 画像診断
  滝口 満喜, 動物臨床医学会年次大会プロシーディング, 37回, 1, 194, 194, Nov. 2016
  動物臨床医学会, Japanese
• もう一度見直す超音波検査法 基本テクニックと各種疾患の診断・評価法 腹部エコー診断がどこまで治療に役立つか
  滝口 満喜, 動物臨床医学会年次大会プロシーディング, 37回, 1, 341, 341, Nov. 2016
  動物臨床医学会, Japanese
• Post‐capillary肺高血圧症ではPre‐capillary肺高血圧症ほど右室機能指標は減弱しない
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Suppl.2, 228, 228, Nov. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• 3次元培養でのシスプラチンと超音波 マイクロバブルの併用
  佐々木 東; Moonen Chrit; 滝口 満喜, 超音波医学, 43, 5, 673, 673, Sep. 2016
  (公社)日本超音波医学会, Japanese
• 超音波による細胞周期変動を利用した増感放射線療法の可能性
  藤沢 眞代; 佐々木 東; 安井 博宣; 工藤 信樹; 滝口 満喜, 超音波医学, 43, 5, 679, 679, Sep. 2016
  (公社)日本超音波医学会, Japanese
• 膀胱癌を模した3次元培養系でのシスプラチンデリバリー
  佐々木 東; 工藤 信樹; 滝口 満喜, 超音波医学, 43, 5, 679, 679, Sep. 2016
  (公社)日本超音波医学会, Japanese
• 3次元培養でのシスプラチンと超音波 マイクロバブルの併用
  佐々木 東; Moonen Chrit; 滝口 満喜, 超音波医学, 43, 5, 673, 673, Sep. 2016
  (公社)日本超音波医学会, Japanese
• 超音波による細胞周期変動を利用した増感放射線療法の可能性
  藤沢 眞代; 佐々木 東; 安井 博宣; 工藤 信樹; 滝口 満喜, 超音波医学, 43, 5, 679, 679, Sep. 2016
  (公社)日本超音波医学会, Japanese
• 膀胱癌を模した3次元培養系でのシスプラチンデリバリー
  佐々木 東; 工藤 信樹; 滝口 満喜, 超音波医学, 43, 5, 679, 679, Sep. 2016
  (公社)日本超音波医学会, Japanese
• 心エコー図検査にて急性期および回復後の変化を捉えた急性肺血栓塞栓症の犬の1症例
  森田智也; 中村健介; 森下啓太郎; 竹内恭介; 大菅辰幸; 那須香菜子; 華園究; 滝口満喜, 北海道獣医師会雑誌, 60, 8, 417, 417, 10 Aug. 2016
  (公社)北海道獣医師会, Japanese
• 好塩基球性白血病の犬の1症例
  渡邉清正; 森下啓太郎; 中村健介; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 60, 8, 406, 406, Aug. 2016
  (公社)北海道獣医師会, Japanese
• 大腸血管拡張症の犬の1症例
  竹内恭介; 大田寛; 高木哲; 中島彩乃; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 60, 8, 418, 418, Aug. 2016
  (公社)北海道獣医師会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープにおけるCOX‐2発現とToll‐like receptorとの関連性の解析
  長谷川純也; 大田寛; 横山望; 笹岡一慶; NISA Khoirun; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 日本獣医学会学術集会講演要旨集, 159回, 456, 456, Aug. 2016
  (公社)日本獣医学会, Japanese
• 犬の免疫介在性関節炎の疫学的および臨床的特徴
  永田 矩之; 中村 健介; 森下 啓太郎; 越後 良介; 奥村 正裕; 佐々木 東; 大田 寛; 滝口 満喜, 北海道獣医師会雑誌, 60, 8, 416, 416, Aug. 2016
  (公社)北海道獣医師会, Japanese
• ネコにおけるポリ塩化ビフェニル(PCBs)の異物代謝機構解明と甲状腺ホルモンへの影響評価
  水川葉月; 前原美咲; 横山望; 市居修; 滝口満喜; 野見山桂; 西川博之; 池中良徳; 中山翔太; 高口倖暉; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 25th, ROMBUNNO.1A‐13, 06 Jun. 2016
  Japanese
• メタボローム解析を用いたイエネコにおけるPCBsの毒性影響評価
  西川博之; 野見山桂; 水川葉月; 横山望; 市居修; 滝口満喜; 石塚真由美; 池中良徳; 中山翔太; 江口哲史; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 25th, ROMBUNNO.1A‐12, 06 Jun. 2016
  Japanese
• 犬猫の脳疾患における造影FLAIRの使用経験
  華園究; 大田寛; 山根由久; 那須香菜子; 細谷兼次; 石塚友人; 伊丹貴晴; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Supplement 1, 335, 335, 05 Jun. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• ポリ塩化ビフェニル(PCBs)のin vivo暴露によるネコの異物代謝解明と甲状腺ホルモンへの影響評価
  水川 葉月; 前原 美咲; 横山 望; 市居 修; 滝口 満喜; 野見山 桂; 西川 博之; 池中 良徳; 中山 翔太; 高口 倖暉; 田辺 信介; 石塚 真由美, The Journal of Toxicological Sciences, 41, Suppl., S215, S215, Jun. 2016
  (一社)日本毒性学会, Japanese
• Parametric Micro flow imaging法を用いて肝実質の動脈化を検出した門脈血栓症の犬の1例
  田村昌大; 中村健介; 森俊輔; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Suppl.1, 335, 335, Jun. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• 間質性肺疾患による重度肺高血圧症を呈したウェルシュ・コーギーの2例
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Suppl.1, 235, 235, Jun. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• 造影超音波検査により計測した肺循環時間と慢性房室弁疾患の重症度との関係
  池田正悟; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 47, Suppl.1, 323, 323, Jun. 2016
  (一社)日本獣医麻酔外科学会, Japanese
• In vivo analysis of metabolic capacities and effects on the thyroid hormone and in cats treated with polychlorinated biphenyls
  MIZUKAWA Hazuki; MAEHARA Misaki; YOKOYAMA Nozomu; ICHII Osamu; TAKIGUCHI Mitsuyoshi; NOMIYAMA Kei; NISHIKAWA Hiroyuki; IKENAKA Yoshinori; NAKAYAMA Shouta M.M; TAKAGUCHI Kohki; TANABE Shinsuke; ISHIZUKA Mayumi, Annual Meeting of the Japanese Society of Toxicology, 43, 0, P, 6, Jun. 2016
  ポリ塩化ビフェニル（PCBs）の水酸化代謝物であるOH-PCBsは、肝臓内で薬物代謝酵素より生成され、その後体外へ排泄される。しかしながら、一部の水酸化代謝物は甲状腺ホルモン（TH）と類似の構造をもつため、THの恒常性を撹乱することが危惧されている。これまでに、多様な陸棲哺乳類の血中OH-PCBsを分析したところ、種間でOH-PCBsの組成に差異が認められ、中でも、ネコのOH-PCBs残留パターンは他種と大きく異なることから、本種は特異な代謝機能を有することが示唆された。しかし、ネコの異物代謝能の研究は僅かであり、化学物質暴露による毒性影響も不明な点が多い。本研究では、ネコにおけるPCBs <i>in vivo</i>暴露試験を実施し、体内動態および代謝に関与する酵素活性や遺伝子を解析するとともに、化学物質の暴露評価に繋がる基盤的情報の収集を目的とした。<br>コーン油に溶解した12異性体のPCBsを腹腔内投与し、経時採血した血清中PCBsおよびOH-PCBs濃度について同条件で実施したイヌの<i>in vivo</i>試験と比較した結果、異性体の残留パターンや体内動態にイヌとネコで種差が観察された。とくにネコでは低塩素化体の残留が顕著であった。また、代謝酵素活性および遺伝子解析の結果、PCBs暴露によりEROD、MROD、PROD活性は上昇するものの、第2相抱合酵素（UGTやSULT）活性は変化せず、PCBs暴露による抱合酵素活性への影響もみられなかった。また、<i>CYP1A1</i>および<i>CYP1A2</i>遺伝子の発現量の上昇も認められた。<br> 本研究により、ネコのPCBs吸収・代謝・排泄能はイヌと異なることが示唆され、とくに低塩素化OH-PCBsの毒性リスクは高いことが予想された。低塩素化OH-PCBsは血中でTH輸送タンパクとの競合結合や、THの硫酸抱合排泄の阻害、TH起因性遺伝子の転写抑制などが報告されており、ネコの甲状腺機能障害が懸念される。, 日本毒性学会, Japanese
• 犬の膵炎診断における客観的で簡便な指標による超音波検査の有用性の検討
  竹内 恭介; 中村 健介; 森下 啓太郎; 大田 寛; 佐々木 東; 滝口 満喜, 日本獣医師会雑誌, 69, 5, 292, 292, May 2016
  (公社)日本獣医師会, Japanese
• 右心機能の低下は僧帽弁疾患犬の生存期間を短縮する
  森田 智也; 中村 健介; 大菅 辰幸; 佐々木 東; 大田 寛; 滝口 満喜, 日本獣医師会雑誌, 69, 5, 294, 294, May 2016
  (公社)日本獣医師会, Japanese
• 日本の専門家が結集して作る獣医臨床腫瘍学テキストブック 消化器系(食道〜結腸・直腸、肛門) 概要
  滝口 満喜, Veterinary Oncology, 3, 2, 78, 84, Apr. 2016
  (株)エデュワードプレス, Japanese
• シスプララチンデリバリーの効果が及ぶ距離
  佐々木東; 工藤信樹; 中村健介; 森下啓太郎; 大田寛; 滝口満喜, 超音波医学, 43, Suppl., S544, S544, Apr. 2016
  (公社)日本超音波医学会, Japanese
• 有機ハロゲン化合物によるコンパニオンアニマルの汚染実態と甲状腺ホルモンへの影響評価
  野見山桂; 山本恭央; 水川葉月; 江口哲史; 滝口満喜; 中山翔太; 池中良徳; 石塚真由美; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 25th, 2016
• 本当にソノポレーションか?
  佐々木東; 工藤信樹; 中山翔太; 滝口満喜, 日本超音波医学会光超音波画像研究会プログラム・抄録集, 2016, 2nd, 22‐24, 2016
  Japanese
• 犬の急性および慢性膵炎の診断治療の最前線 膵炎の画像診断と生検診断
  滝口 満喜, 動物臨床医学, 24, 4, 153, 154, Dec. 2015
  動物臨床医学会, Japanese
• 右心室‐左心室‐左心房interaction:犬急性肺高血圧症モデルにおける検討
  大菅辰幸; 中村健介; 森田智也; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Suppl.2, 251, 251, Dec. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 診察中に左房破裂による心タンポナーデを発症したものの内科療法のみで長期生存中の犬の1例
  川元誠; 中村健介; 高瀬奈美; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Suppl.2, 241, 241, Dec. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 二次診療施設において診断・治療に苦慮した消化器疾患の症例カンファレンス 膵臓疾患編
  滝口 満喜, 動物臨床医学会年次大会プロシーディング, 36回, 1, 125, 125, Nov. 2015
  動物臨床医学会, Japanese
• 胆管原発カルチノイドによる肝外胆管閉塞と診断した猫の1例
  森俊輔; 中村健介; 高木哲; 賀川由美子; 森下啓太郎; 大田寛; 滝口満喜, 動物臨床医学会年次大会プロシーディング, 36回, 2, 283, 284, Nov. 2015
  動物臨床医学会, Japanese
• 犬の非再生性免疫介在性貧血(NRIMA)に対する治療の前向き検討
  森下啓太郎; 竹内恭介; 森俊輔; 早川小百合; 中村健介; 佐々木東; 大田寛; 滝口満喜, 動物臨床医学会年次大会プロシーディング, 36回, 2, 367, 368, Nov. 2015
  動物臨床医学会, Japanese
• 犬のびまん性肝疾患に対する造影超音波検査の応用の試み
  道下 明日香; 森下 啓太郎; 華園 究; 中村 健介; 滝口 満喜; 高木 哲, 日本獣医画像診断学会抄録, 58回, 12, 12, Sep. 2015
  (一社)日本獣医画像診断学会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるToll‐like receptor2,4の局在解析
  横山望; 大田寛; 賀川由美子; 山崎淳平; 市居修; KHOIRUN Nisa; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 日本獣医学会学術集会講演要旨集, 158th, 402, 402, 30 Aug. 2015
  (公社)日本獣医学会, Japanese
• 胸腺腫に続発した後天性重症筋無力症の猫の1例
  早川小百合; 大田寛; 細谷謙次; 賀川由美子; 華園究; 田淵祐司; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 403, 403, 10 Aug. 2015
  (公社)北海道獣医師会, Japanese
• 【一目でわかる症候シリーズvol.4 嘔吐(1)】胃の腫瘍
  滝口 満喜, SA Medicine, 17, 4, 18, 21, Aug. 2015
  (株)エデュワードプレス, Japanese
• (パート1)参加型臨床実習をどうするか 目前にせまった参加型臨床実習の内容、実施方法を議論する 伴侶動物の参加型臨床実習 国立大学編国立大学の参加型臨床実習の実施方針、実際の実施計画(伴侶動物)「国際認証を目指す伴侶動物の参加型臨床実習」
  滝口 満喜, 日本獣医学会学術集会講演要旨集, 158回, 139, 139, Aug. 2015
  (公社)日本獣医学会, Japanese
• 汎用性が高く臨床的にも有用な犬の左心房機能評価法の検討;僧帽弁逆流の重症度評価について
  川元誠; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 390, 390, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 右心機能の低下は僧帽弁疾患犬の生存期間を短縮する
  森田智也; 中村健介; 大菅辰幸; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 389, 389, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 超低脂肪食で治療した犬慢性腸症18例の回顧的検討
  横山望; 大田寛; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 394, 394, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 犬の膵炎診断における客観的で簡便な指標による超音波検査の有用性の検討
  竹内恭介; 中村健介; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 北海道獣医師会雑誌, 59, 8, 396, 396, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 心エコー図検査による右心機能指標と心臓カテーテル検査との関連:急性肺高血圧モデル犬を用いた検討
  森田智也; 中村健介; 大菅辰幸; 森下啓太郎; 佐々木東; 大田寛; 滝口満喜, 日本獣医学会学術集会講演要旨集, 158回, 403, 403, Aug. 2015
  (公社)日本獣医学会, Japanese
• 免疫抑制治療中に合併したRgizopus microsporus感染に伴う胃穿孔の犬の1例
  那須 香菜子; 中村 健介; 高木 哲; 賀川 由美子; 青島 圭佑; 甲斐 千暁; 後藤 正光; 滝口 満喜, 北海道獣医師会雑誌, 59, 8, 392, 392, Aug. 2015
  (公社)北海道獣医師会, Japanese
• 【副腎腫瘍】副腎腫瘍の内科
  滝口 満喜, Joncol: Japanese Journal of Veterinary Clinical Oncology, 11, 2, 22, 24, Jul. 2015
  (株)ファームプレス, Japanese
• Dynamic CTによる肝細胞性腫瘤の良悪性鑑別
  那須香菜子; 中村健介; 高木哲; 細谷謙次; 華園究; 賀川由美子; 森下啓太郎; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Supplement 1, 302, 302, 10 Jun. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 右室Tei‐indexは僧帽弁疾患犬の1年生存を規定する
  森田智也; 中村健介; 大菅辰幸; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Suppl.1, 280, 280, Jun. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 僧帽弁疾患犬におけるうっ血性心不全の発症はLA strain法によるブースターポンプ機能指標と関連する
  川元誠; 中村健介; 大菅辰幸; 森田智也; 佐々木東; 森下啓太郎; 大田寛; 滝口満喜, 日本獣医麻酔外科学雑誌, 46, Suppl.1, 281, 281, Jun. 2015
  (一社)日本獣医麻酔外科学会, Japanese
• 肝静脈造影超音波検査による肝疾患罹患犬の肝内血行動態の評価
  森下 啓太郎; 平本 彰; 大菅 辰幸; 中村 健介; 大田 寛; 滝口 満喜, 日本獣医師会雑誌, 68, 5, 326, 327, May 2015
  (公社)日本獣医師会, Japanese
• ドラッグデリバリーを可視化する培養系の開発
  佐々木 東; 滝口 満喜; Bos Clemens; Moonen Chrit, 超音波医学, 42, Suppl., S476, S476, Apr. 2015
  (公社)日本超音波医学会, Japanese
• ドラッグデリバリーを可視化する培養系の開発
  佐々木 東; 滝口 満喜; Bos Clemens; Moonen Chrit, 超音波医学, 42, Suppl., S476, S476, Apr. 2015
  (公社)日本超音波医学会, Japanese
• 肝静脈造影超音波検査による肝疾患罹患犬の肝内血行動態の評価
  森下啓太郎; 平本彰; 大菅辰幸; 中村健介; 大田寛; 滝口満喜, 日本獣医師会獣医学術学会年次大会講演要旨集, 2014, 137, 30 Jan. 2015
  Japanese
• ペットネコにおける有機ハロゲン化合物および水酸化代謝物の汚染実態と甲状腺ホルモン恒常性への影響
  山本恭央; 野見山桂; 水川葉月; 滝口満喜; 石塚真由美; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 24th, 2015
• ペット動物における有機ハロゲン化合物および水酸化代謝物の曝露実態と甲状腺ホルモン恒常性へ及ぼす影響
  山本恭央; 野見山桂; 水川葉月; 滝口満喜; 石塚真由美; 国末達也; 田辺信介, 日本内分泌かく乱化学物質学会研究発表会要旨集, 18th, 124, 124, 2015
  環境ホルモン学会(日本内分泌撹乱化学物質学会), Japanese
• 犬におけるSpeckle tracking法を用いた右室strain解析の再現性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.2, 216, 216, Dec. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の急性および慢性膵炎の診断治療の最前線―膵炎の画像診断と生検診断―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35th, 1, 209, 210, 14 Nov. 2014
  動物臨床医学会, Japanese
• うっ血性左心不全を呈した原発性心臓血管肉腫の犬の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; TOOYAMA NOBUO; MORITA TOMOYA; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 33, 34, Nov. 2014
  動物臨床医学会, Japanese
• 免疫抑制量のプレドニゾロンが奏功した重度肺高血圧症を伴う間質性肺疾患を疑ったウェルシュコーギーの1例
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 103, 104, Nov. 2014
  動物臨床医学会, Japanese
• NSAIDsが原因と考えられた骨髄壊死の犬の1例
  MORISHITA KEITARO; DEGUCHI TATSUYA; KAGAWA YUMIKO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 35回, 2, 93, 94, Nov. 2014
  動物臨床医学会, Japanese
• 犬慢性消化器疾患における十二指腸造影超音波検査の有用性の検討
  篠原 正義; リン・スーイー; 森下 啓太郎; 横山 望; 大菅 辰幸; 中村 健介; 賀川 由美子; 大田 寛; 滝口 満喜, 日本獣医画像診断学会抄録, 56回, 24, 24, Sep. 2014
  日本獣医画像診断学会, Japanese
• 参加型臨床実習 小動物
  TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 157th, 192, 192, 11 Aug. 2014
  (公社)日本獣医学会, Japanese
• 参加型臨床実習 小動物
  TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 398, 398, 10 Aug. 2014
  (公社)北海道獣医師会, Japanese
• プロカルバジンが奏功した肉芽腫性髄膜脳脊髄炎を疑う犬の1例
  YAMAGUCHI TOMOO; OTA HIROSHI; MATSUMOTO MASANORI; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 365, 365, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 肺高血圧症の犬に対するDual pulsed‐wave Doppler法を用いた右室Tei‐index測定の有用性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 369, 369, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 尿管結石による水腎症から膿腎症に至った犬の1例
  HAYAKAWA SAYURI; NAKAMURA KENSUKE; TAKAGI SATOSHI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 376, 376, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 二次的な巨大食道を伴った鼻咽頭ポリープの猫の1例
  NASU KANAKO; NAKAMURA KENSUKE; TAKAGI SATOSHI; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 374, 374, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 活性型ビタミンD₃外用薬の慢性摂取により高カルシウム血症が認められた犬の2例
  SHIMOIDE ARISA; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 379, 379, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 肝静脈造影超音波検査による肝疾患罹患犬の肝内血行動態の評価
  MORISHITA KEITARO; HIRAMOTO AKIRA; RIN SU・I; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 58, 8, 370, 370, Aug. 2014
  (公社)北海道獣医師会, Japanese
• 左心房機能は心血管薬によりいかに変化するか:正常犬における2D Speckle Tracking法を用いた検討
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORITA TOMOYA; YEE LIM SUE; YOKOYAMA NOZOMU; MORISHITA KEITARO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 157回, 479, 479, Aug. 2014
  (公社)日本獣医学会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるclaudinの蛋白質発現解析
  YOKOYAMA NOZOMU; OTA HIROSHI; MORITA TOMOYA; OSUGA TATSUYUKI; LIM SUE YEE; MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 157回, 491, 491, Aug. 2014
  (公社)日本獣医学会, Japanese
• 犬バベハア症治療における熱ショック蛋白質90の薬剤標的としての可能性
  ABE MOEKO; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 157回, 361, 361, Aug. 2014
  (公社)日本獣医学会, Japanese
• 犬の非感染性髄膜脳炎症例の脳脊髄液中におけるサイトカインの発現解析
  OTA HIROSHI; KAMADA MAYUMI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医神経病学会, 40th, 19, 05 Jul. 2014
  Japanese
• 犬胃癌10症例における超音波検査所見
  YOKOYAMA NOZOMI; OTA HIROSHI; NAKAMURA KENSUKE; MORISHITA KEITARO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 261, 261, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 造影超音波検査による腎機能評価の基礎的検討
  MORISHITA KEITARO; RIN SU・I; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 262, 262, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 徐脈性不整脈に対してピモベンダンを投与し失神の改善を認めた犬の1例
  MORITA TOMOYA; SUZUKI SHUJI; NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 45, Suppl.1, 248, 248, Jun. 2014
  (一社)日本獣医麻酔外科学会, Japanese
• 2D Speckle Tracking法を用いた左心房機能評価による犬心筋症の重症度評価
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; SUZUKI SHUJI; MORISHITA KEITARO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 67, 5, 367, 367, 20 May 2014
  (公社)日本獣医師会, Japanese
• プレドニゾロンを主とする免疫抑制療法実施症例における犬膵特異的リパーゼの推移
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 67, 5, 369, 369, May 2014
  (公社)日本獣医師会, Japanese
• セルレイン誘発膵炎犬における造影超音波検査の定量的および定性的評価
  LIM SUE YEE; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医畜産新報, 67, 4, 286, 288, Apr. 2014
  文永堂出版(株), Japanese
• 北海道での小動物臨床での放射線利用の広がり
  TAKIGUCHI MITSUYOSHI, 日本アイソトープ協会放射線安全取扱部会年次大会要旨集, 2014, 57, 2014
  Japanese
• LC-MS/MS Analysis of Canine Lipoproteins Fractionated Using the Ultracentrifugation-Precipitation Method (vol 75, pg 1471, 2013)
  Asuka Suto; Masahiro Yamasaki; Yukari Takasaki; Yasuyuki Fujita; Riichiro Abe; Hiroshi Shimizu; Hiroshi Ohta; Mitsuyoshi Takiguchi, JOURNAL OF VETERINARY MEDICAL SCIENCE, 75, 12, 1678, 1678, Dec. 2013
  English, Others
• Dual pulsed‐wave doppler法による右室Tei‐index測定の再現性
  MORITA TOMOYA; NAKAMURA KENSUKE; OSUGA TATSUYUKI; SUZUKI SHUJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 44, Suppl.2, 250, 250, Dec. 2013
  (一社)日本獣医麻酔外科学会, Japanese
• 猫心筋症において2D Speckle Tracking法を用いた左心房機能評価は可能であるのか
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; SUZUKI SHUJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 44, Suppl.2, 249, 249, Dec. 2013
  (一社)日本獣医麻酔外科学会, Japanese
• 両側性尿管閉塞を呈した犬の1例
  TOOYAMA NOBUO; NAKAMURA KENSUKE; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; TAKAGI SATOSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 3, 4, Nov. 2013
  動物臨床医学会, Japanese
• 非典型的溶血性尿毒症症候群(D‐HUS)が疑われた犬の1例
  MORISHITA KEITARO; TOOYAMA NOBUO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 141, 142, Nov. 2013
  動物臨床医学会, Japanese
• 慢性特発性偽性腸閉塞症が疑われた犬の1例
  YOKOYAMA NOZOMU; OTA HIROSHI; HASHIBA KANO; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OCHIAI KENJI; TERAMOTO HIDESHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 59, 60, Nov. 2013
  動物臨床医学会, Japanese
• 市販フードの給餌によって病的骨折を起こした栄養性二次性上皮小体機能亢進症の犬の1例
  HASHIBA KANO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 119, 120, Nov. 2013
  動物臨床医学会, Japanese
• 組織奇形が疑われた膀胱粘膜下嚢胞の犬の1例
  MORISHITA KEITARO; HOSOYA KENJI; OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 34回, 2, 9, 10, Nov. 2013
  動物臨床医学会, Japanese
• 超音波検査により診断が可能であった子宮捻転の犬の1例
  中村 健介; 山崎 真大; 大田 寛; 森下 啓太郎; 木村 享史; 滝口 満喜, 日本獣医画像診断学会抄録, 54回, 28, 28, Sep. 2013
  日本獣医画像診断学会, Japanese
• 胆嚢十二指腸吻合および胆嚢肝管吻合を行った胆管閉塞の猫の1症例
  ANEGAWA HIROFUMI; TAKAGI SATOSHI; HASHIBA KANO; HOSOYA KENJI; HOSHINO YUKI; TAKIGUCHI MITSUYOSHI; OKUMURA MASAHIRO, 北海道獣医師会雑誌, 57, 8, 400, 400, 10 Aug. 2013
  (公社)北海道獣医師会, Japanese
• プレドニゾロンを主とする免疫抑制療法実施症例における犬膵特異的リパーゼの推移
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 402, 402, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 輪状咽頭アカラシアの犬の1例
  UENO TAKASHI; OTA HIROSHI; TAKAGI SATOSHI; HOSHI KIYOTAKA; MORISHITA KEITARO; NAKAMURA TAKESHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 401, 401, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 2D Speckle Tracking法を用いた左心房機能評価による犬心筋症の重症度評価
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; HOSOYA KENJI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 396, 396, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 非典型的溶血性尿毒症症候群(D‐HUS)が疑われた犬の1例
  TOOYAMA NOBUO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 395, 395, Aug. 2013
  (公社)北海道獣医師会, Japanese
• 閉鎖式持続吸引ドレーンにて良好にコントロールできた膵膿瘍の犬の1例
  YAMAGUCHI TOMOO; MORISHITA KEITARO; HOSOYA KENJI; MUKAIDANI RYUSUKE; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 57, 8, 400, 400, Aug. 2013
  (公社)北海道獣医師会, Japanese
• ジミナゼン耐性Babesia gibsoniの熱ショック蛋白質90遺伝子発現量の測定
  IDAKA NATSUKI; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 156回, 236, 236, Aug. 2013
  (公社)日本獣医学会, Japanese
• 特発性収縮性心膜炎の犬の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; HOSOYA KENJI; MORITA TOMOYA; KAGAWA YUMIKO; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 44, Suppl.1, 245, 245, Jun. 2013
  (一社)日本獣医麻酔外科学会, Japanese
• QUALITATIVE AND QUANTITATIVE CONTRAST ENHANCED ULTRASONOGRAPHIC ASSESSMENT OF CERULEIN-INDUCED PANCREATITIS IN DOGS
  S. Y. Lim; K. Nakamura; K. Morishita; H. Ohta; M. Yamasaki; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 27, 3, 707, 707, May 2013
  English, Summary international conference
• MARKED INCREASED EXPRESSION OF INTERLEUKIN 8 IN THE COLORECTAL MUCOSA OF DOGS WITH INFLAMMATORY COLORECTAL POLYPS BUT NOT WITH LYMPHOCYTIC-PLASMACYTIC COLITIS
  Y. Tamura; H. Ohta; N. Yokoyama; K. Morishita; K. Nakamura; M. Yamasaki; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 27, 3, 706, 706, May 2013
  English, Summary international conference
• モバイル超音波時代の幕開け
  TAKIGUCHI MITSUYOSHI, MVM, 22, 4, 61, 63, 01 May 2013
  (株)ファームプレス, Japanese
• 肝静脈血流波形解析による右心不全の重症度評価
  NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会雑誌, 66, 5, 346, 347, May 2013
  (公社)日本獣医師会, Japanese
• 内科領域の取り組みと課題
  TSUJIMOTO HAJIME; OKUDA MASARU; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 155th, 150, 150, 04 Mar. 2013
  (公社)日本獣医学会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープの病態
  OTA HIROSHI; TAMURA YU; YOKOYAMA NOZOMI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 155回, 153, 153, Mar. 2013
  (公社)日本獣医学会, Japanese
• Babesia gibsoni熱ショック蛋白質90の遺伝子クローニングおよび系統樹解析
  YAMASAKI MASAHIRO; TSUBOI YOSHIHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本寄生虫学会大会プログラム・抄録集, 82nd, 84, Feb. 2013
  Japanese
• “診る目を養う”超音波検査 第12回 膵臓を極める
  TAKIGUCHI MITSUYOSHI, SA Med, 15, 1, 18, 22, 01 Feb. 2013
  (株)エデュワードプレス, Japanese
• 開業獣医師のための画像診断再入門 実例から学ぶ画像診断ピットフォール
  TAKIGUCHI MITSUYOSHI, MVM, 22, 1, 31, 36, 01 Jan. 2013
  (株)ファームプレス, Japanese
• ―免疫抑制剤の理論と実践―炎症性腸疾患の概要と免疫抑制剤使用の考え方
  TAKIGUCHI MITSUYOSHI, CAP (Companion Anim Pract), 27, 282, 19, 23, 01 Dec. 2012
  (株)緑書房, Japanese
• 末梢肺動脈狭窄症と診断した高齢猫の1例
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.2, 213, 213, Dec. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• 最近よく診る消化器疾患の診断と治療―IBD,胃運動性障害等の対策について―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 33rd, 3, 223, 225, 16 Nov. 2012
  動物臨床医学会, Japanese
• Babesia gibsoniにおけるdiminazene aceturate耐性の性質の安定性
  B.K.・ウィクラマセカラ・ラジャパクシャゲ; 山崎 真大; S.J.・ファン; 中村 健介; 佐々木 東; 村上 正紘; 田村 悠; S.Y.・リン; 大田 寛; 滝口 満喜, 獣医寄生虫学会誌, 11, 1, 35, 35, Oct. 2012
  日本獣医寄生虫学会, Japanese
• “診る目を養う”超音波検査 第11回 内分泌疾患を超音波で診る(2)甲状腺・上皮小体
  TAKIGUCHI MITSUYOSHI, SA Med, 14, 5, 81, 87, 01 Oct. 2012
  (株)エデュワードプレス, Japanese
• ソナゾイド造影超音波検査と機械学習を組み合わせた脾臓腫瘤の自動良悪性鑑別の試み
  中村 健介; 滝口 満喜; 高木 一也; 近藤 敏志, 日本獣医画像診断学会抄録, 52回, 16, 16, Sep. 2012
  (一社)日本獣医画像診断学会, Japanese
• 北海道の飼育犬におけるCapnocytophaga属菌の侵淫調査
  HATANAKA YUI; TAKIGUCHI MITSUYOSHI; GOTO MASAMITSU; MURAMATSU YASUKAZU, 日本獣医学会学術集会講演要旨集, 154th, 270, 270, 31 Aug. 2012
  (公社)日本獣医学会, Japanese
• 披裂軟骨背側に形成された炎症性腫瘤病変を喉頭切開により摘出した猫の1例
  IWANO KIMI; TAKAGI SATOSHI; HOSHINO YUKI; HOSOYA KENJI; TAKIGUCHI MITSUYOSHI; OKUMURA MASAHIRO, 北海道獣医師会雑誌, 56, 8, 411, 411, 10 Aug. 2012
  (公社)北海道獣医師会, Japanese
• 好酸球増多症候群を伴った消化器型リンパ腫の猫の1例
  TOOYAMA NOBUO; HOSOYA KENJI; NAKAMURA KENSUKE; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 399, 399, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 下垂体性副腎皮質機能亢進症とクロム親和性細胞腫を併発した犬の1例
  KANAZAWA TOMONORI; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 410, 410, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 麻酔後の全脳虚血が疑われた2症例のMRI所見
  OTA HIROSHI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 429, 429, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 肝静脈血流波形解析による右心不全の重症度評価
  NAKAMURA KENSUKE; OSUGA TATSUYUKI; MORISHITA KEITARO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 409, 409, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 両側性子宮分節性無形成から片側性子宮角捻転に至った犬の1例
  YOKOYAMA NOZOMI; NAKAMURA KENSUKE; KIMURA TAKASHI; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 418, 418, Aug. 2012
  (公社)北海道獣医師会, Japanese
• 下垂体性副腎皮質機能亢進症と中枢性尿崩症を併発し,放射線治療を行った下垂体腫瘍の犬の1例
  TAMURA YU; YAMASAKI MASAHIRO; MORISHITA KEITARO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 56, 8, 410, 410, Aug. 2012
  (公社)北海道獣医師会, Japanese
• Diminazene aceturate耐性Babesia gibsoniの核酸取り込み量の測定
  WATANABE NATSUO; YAMASAKI MASAHIRO; KUMARA W; R. BANDULA; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 154回, 215, 215, Aug. 2012
  (公社)日本獣医学会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ病変における炎症性サイトカインの発現解析
  TAMURA YU; OTA HIROSHI; MURAKAMI MASAHIRO; BANDULA KUMARA WR; SUE YEE LIM; OSUGA TATSUYUKI; MORISHITA KEITARO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 154回, 307, 307, Aug. 2012
  (公社)日本獣医学会, Japanese
• 腎細胞癌移植マウスにおけるマルチキナーゼ阻害剤Sorafenib治療後のFLTによる治療反応評価
  MURAKAMI MASAHIRO; CHO SHOKICHI; CHO GEN; CHODORI NUSURATTOFATIMA; CHODORI NUSURATTOFATIMA; U BUMBUN; NISHIJIMA KEN'ICHI; KUBO NAOKI; TAKIGUCHI MITSUYOSHI; TAMAKI NAGARA; KUGE YUJI, 核医学, 49, 3, S246, S246, Aug. 2012
  (一社)日本核医学会, Japanese
• 犬の頭蓋内疾患診断における経頭蓋超音波ドプラ法の有用性の検討
  SASAOKA KAZUYOSHI; NAKAMURA KENSUKE; MORISHITA KEITARO; OSUGA TATSUYUKI; LIM SUE YEE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 270, 270, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• CT検査を実施した肝葉捻転の犬の2例
  MORISHITA KEITARO; HOSOYA KENJI; TAKAGI SATOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 267, 267, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• 房室ブロックの犬2例に認められた低速僧帽弁逆流と高速左室駆出血流
  NAKAMURA KENSUKE; TOOYAMA NOBUO; UENO TAKASHI; MORISHITA KEITARO; OSUGA TATSUYUKI; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 43, Suppl.1, 245, 245, Jun. 2012
  (一社)日本獣医麻酔外科学会, Japanese
• “診る目を養う”超音波検査 第10回 内分泌疾患を超音波で診る(1)副腎を極める
  TAKIGUCHI MITSUYOSHI, SA Med, 14, 3, 68, 73, 01 Jun. 2012
  (株)エデュワードプレス, Japanese
• LEFT ATRIAL FUNCTION ANALYSIS BY LEFT ATRIAL TRACKING METHOD IN CANINE CHRONIC MITRAL VALVULAR HEART DISEASE.
  K. Nakamura; T. Osuga; M. Yamasaki; H. Ohta; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 26, 3, 720, 720, May 2012
  English, Summary international conference
• INTERLEUKIN-17A GENE EXPRESSION IN THE INTESTINAL MUCOSA IN DOGS WITH LYMPHOCYTIC-PLASMACYTIC ENTERITIS AND INFLAMMATORY COLORECTAL POLYP
  H. Ohta; K. Takada; Y. Tamura; K. Nakamura; M. Yamasaki; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 26, 3, 772, 773, May 2012
  English, Summary international conference
• ¹⁸F×FMISOを用いた腎細胞癌移植モデルにおける抗血管新生療法(sorafenicの早期治療反応の評価
  MURAKAMI MASAHIRO; CHO GEN; NUSURATTOFATIMA CHODORI; U MOMBUN; NISHIJIMA KEN'ICHI; TAKIGUCHI MITSUYOSHI; TAMAKI NAGARA; KUGE YUJI, JSMI Rep, 5, 2, 117, 117, May 2012
  日本分子イメージング学会, Japanese
• パルス超音波を用いたソノポレーションの実現に向けたいくつかの基礎的検討
  KUDO NOBUKI; SASAKI NOBORU; TAKIGUCHI MITSUYOSHI, 超音波医学, 39, Suppl., S174, S174, 15 Apr. 2012
  (公社)日本超音波医学会, Japanese
• 診断装置によるソノポレーションの特徴と条件設定
  SASAKI NOBORU; NAKAMURA KENSUKE; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 39, 2, 226, 227, 15 Mar. 2012
  (公社)日本超音波医学会, Japanese
• ジミナゼン耐性株および野生株におけるBabesia gibsoniミトコンドリアDNAの塩基配列の比較
  ウイクラマセカラ B.K.; ラジャパク・シャゲ; 山崎 真大; 中村 健介; 佐々木 東; 村上 正紘; 田村 悠; リン S.Y.; 大田 寛; 滝口 満喜, 獣医寄生虫学会誌, 10, 1-2, 34, 34, Mar. 2012
  日本獣医寄生虫学会, Japanese
• Babesia gibsoniにおけるdiminazene aceturate抵抗性の安定性(Stability of characteristic of the diminazene aceturate resistance in Babesia gibsoni)
  Rajapakshage Bandula Kumara Wickramasekara; 山崎 真大; Hwang Shiang-Jyi; 中村 健介; 佐々木 東; 村上 正紘; 田村 悠; Lim Sue Yee; 大田 寛; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 153回, 221, 221, Mar. 2012
  (公社)日本獣医学会, English
• 臨床の立場から臨床薬理学に期待するもの
  TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 153rd, 147, 147, 01 Mar. 2012
  (公社)日本獣医学会, Japanese
• 診断シリーズ vol.1 肝胆道系疾患 X線検査
  TAKIGUCHI MITSUYOSHI, SA Med, 14, 1, 37, 39, 01 Feb. 2012
  (株)エデュワードプレス, Japanese
• 診断シリーズ vol.1 肝胆道系疾患 超音波検査
  TAKIGUCHI MITSUYOSHI, SA Med, 14, 1, 40, 44, 01 Feb. 2012
  (株)エデュワードプレス, Japanese
• ここまでは押さえておこう! 腹部エコー検査
  滝口 満喜, 日本獣医画像診断学会抄録, 50回, 59, 59, Jan. 2012
  (一社)日本獣医画像診断学会, Japanese
• 麻酔後の全脳虚血が疑われた猫2例のMRI所見
  大田 寛; 山崎 真大; 滝口 満喜, 日本獣医画像診断学会抄録, 50回, 94, 94, Jan. 2012
  日本獣医画像診断学会, Japanese
• “診る目を養う”超音波検査 第9回 嘔吐の原因を超音波で探る(4)腸管を極める その2
  TAKIGUCHI MITSUYOSHI, SA Med, 13, 6, 55, 58, 01 Dec. 2011
  (株)エデュワードプレス, Japanese
• 心エコー図検査にて心内膜心筋線維症と診断した猫4例
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; MURAKAMI MASAHIRO; MATSUYAMA ARATA; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 42, Suppl.2, 233, 233, Dec. 2011
  (一社)日本獣医麻酔外科学会, Japanese
• 活性型ビタミンD₃外用薬(マキサカルシトール軟膏)常用患者の飼育犬に認められた高カルシウム血症
  TOOYAMA NOBUO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32nd, 2, 149, 150, 18 Nov. 2011
  動物臨床医学会, Japanese
• 最近よく診る消化器疾患の傾向と対策―確実な診断と治療を行うには―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32nd, 3, 185, 185, 18 Nov. 2011
  動物臨床医学会, Japanese
• 内分泌疾患の画像診断
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32nd, 1, 100, 100, 18 Nov. 2011
  動物臨床医学会, Japanese
• 小型犬における下垂体性副腎皮質機能亢進症の超音波診断基準
  MATSUYAMA ARATA; TOOYAMA NOBUO; MURAKAMI MASAHIRO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32nd, 3, 14, 18, 18 Nov. 2011
  動物臨床医学会, Japanese
• ポリエン系抗生物質アムホテリシンBのイヌバベシア症治療薬としての応用に関する研究
  YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32nd, 3, 91, 94, 18 Nov. 2011
  動物臨床医学会, Japanese
• 超音波診断装置および超音波造影剤による抗がん剤の抗腫瘍効果の増強
  SASAKI NOBORU; NAKAMURA KENSUKE; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 38, 6, 674, 674, 15 Nov. 2011
  (公社)日本超音波医学会, Japanese
• 最近よく診る消化器疾患の診断と治療
  TAKIGUCHI MITSUYOSHI, MVM, 20, 7, 82, 87, 01 Nov. 2011
  (株)ファームプレス, Japanese
• 犬の肝臓腫瘤診断におけるソナゾイド造影超音波検査―4年間の検討―
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; HOSOYA KENJI; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 32回, 3, 6, 9, Nov. 2011
  動物臨床医学会, Japanese
• “診る目を養う”超音波検査 第8回 嘔吐の原因を超音波で探る(3)腸管を極める その1
  TAKIGUCHI MITSUYOSHI, SA Med, 13, 5, 79, 83, 01 Oct. 2011
  (株)エデュワードプレス, Japanese
• セフォベシンが有効であった巨大食道症に併発した誤嚥性肺炎の犬の一例
  TAKIGUCHI MITSUYOSHI, CAP (Companion Anim Pract), 26, 268, 78, 80, 01 Oct. 2011
  (株)緑書房, Japanese
• 犬の嘔吐の治療および管理の実際と対応 制吐剤の効果的な使い方
  滝口 満喜; 大野 耕一; 竹内 和義; 峯岸 則之; 米元 仁, CLINIC NOTE, 7, 9, 77, 83, Sep. 2011
  (株)エデュワードプレス, Japanese
• 病理組織検査にて角層下および真皮への多数の好酸球の浸潤が認められた犬の2症例
  CHIBA ERI; YAMASAKI MASAHIRO; OTA HIROSHI; NAKAMURA KENSUKE; SASAKI NOBORU; MURAKAMI MASAHIRO; TAMURA YU; YEE LIM SUE; KUMARA WICKRAMASEKARA R.B; HONDA HIDETAKA; NASUNO TOYOHIKO; OCHIAI KENJI; TAKIGUCHI MITSUYOSHI, 獣医臨床皮膚科, 17, 3, 177, 182, Sep. 2011
  (一社)日本獣医皮膚科学会, Japanese
• ジミナゼンアセチュレート耐性Babesia gibsoni株と野生型におけるミトコンドリアDNA配列の比較(Comparison of the sequence of mitochondrial DNA from diminazene aceturate resistance Babesia gibsoni strain with those from wild type)
  Rajapakshage Bandula Kumara Wickramasekara; 山崎 真大; 中村 健介; 佐々木 東; 村上 正紘; 田村 悠; Lim Sue Yee; 大田 寛; 滝口 満喜, 日本獣医学会学術集会講演要旨集, 152回, 203, 203, Aug. 2011
  (公社)日本獣医学会, English
• セフォベシンが有効であった好中球性胆管炎の猫の一例
  TAKIGUCHI MITSUYOSHI, CAP (Companion Anim Pract), 26, 266, 84, 86, 01 Aug. 2011
  (株)緑書房, Japanese
• ”診る目を養う”超音波検査 第7回 嘔吐の原因を超音波で探る(2)胃の超音波検査 その2
  TAKIGUCHI MITSUYOSHI, SA Med, 13, 4, 70, 73, 01 Aug. 2011
  (株)エデュワードプレス, Japanese
• 犬の僧房弁閉鎖不全症におけるLeft atrial tracking法を用いた左心房機能評価の有用性
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 55, 8, 386, 386, Aug. 2011
  (公社)北海道獣医師会, Japanese
• 後鼻孔領域に炎症性ポリープを認めた若齢犬の1例
  WATANABE TAKESHI; OTA HIROSHI; SONO FUMIO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 55, 8, 387, 387, Aug. 2011
  (公社)北海道獣医師会, Japanese
• 気腫性胆嚢炎の犬の1例
  TAMURA YU; YAMASAKI MASAHIRO; NAKAMURA KENSUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 55, 8, 386, 386, Aug. 2011
  (公社)北海道獣医師会, Japanese
• 胃癌の犬5例における超音波検査および内視鏡検査所見の特徴
  TOOYAMA NOBUO; OTA HIROSHI; TAKAGI SATOSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 55, 8, 397, 397, Aug. 2011
  (公社)北海道獣医師会, Japanese
• 心エコー図検査とD‐dimer測定により診断し得た肺血栓塞栓症の犬の1例
  OGI NAOKO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 55, 8, 385, 385, Aug. 2011
  (公社)北海道獣医師会, Japanese
• 超音波診断装置および超音波造影剤による抗がん剤のソノポレーションの抗腫瘍効果
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; BANDULA KUMARA WR; LIM SUE YEE; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 152回, 302, 302, Aug. 2011
  (公社)日本獣医学会, Japanese
• 犬の慢性腸炎症例の腸粘膜におけるIL‐17A遺伝子の発現解析
  TAKADA KANAE; OTA HIROSHI; NAKAMURA KENSUKE; SASAKI NOBORU; MURAKAMI MASAHIRO; KUMARA W.R. BANDULA; TAMURA YU; LIM SUE YEE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 152回, 298, 298, Aug. 2011
  (公社)日本獣医学会, Japanese
• プロテオーム解析を用いた犬リポ蛋白質測定法の評価
  SUDO ASUKA; YAMASAKI MASAHIRO; NAKAMURA KENSUKE; SASAKI NOBORU; MURAKAMI MASAHIRO; KUMARA W.R. BANDULA; TAMURA YU; LIM SUE YEE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 152回, 291, 291, Aug. 2011
  (公社)日本獣医学会, Japanese
• 犬の慢性腸炎症例の結腸粘膜における細胞接着分子クローディンの発現変動
  OTA HIROSHI; TAMURA YU; LIM SUE YEE; KUMARA WR BANDULA; MURAKAMI MASAHIRO; SASAKI NOBORU; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 152回, 298, 298, Aug. 2011
  (公社)日本獣医学会, Japanese
• 門脈体循環シャント(前編)門脈体循環シャントの画像診断:超音波検査
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE, Surgeon, 15, 4, 33, 38, 01 Jul. 2011
  (株)エデュワードプレス, Japanese
• ”診る目を養う”超音波検査 第6回 嘔吐の原因を超音波で探る(1)胃の超音波検査
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE, SA Med, 13, 3, 72, 78, 01 Jun. 2011
  (株)エデュワードプレス, Japanese
• 治療シリーズ Vol.10―私はこうしている―「肝胆道系・膵外分泌疾患」胆嚢炎
  TAKIGUCHI MITSUYOSHI, SA Med, 13, 3, 50, 51, 01 Jun. 2011
  (株)エデュワードプレス, Japanese
• 犬の心疾患におけるLeft atrial tracking法を用いた左心房機能評価の有用性
  OSUGA TATSUYUKI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 42, Suppl.1, 243, 243, Jun. 2011
  (一社)日本獣医麻酔外科学会, Japanese
• 治療シリーズ Vol.10―私はこうしている―「肝胆道系・膵外分泌疾患」胆石症
  TAKIGUCHI MITSUYOSHI, SA Med, 13, 3, 51, 52, 01 Jun. 2011
  (株)エデュワードプレス, Japanese
• 仮想気管支鏡検査が診断に有用であった肺葉捻転の犬の2例
  MURAKAMI MASAHIRO; NAKAMURA KENSUKE; SASAKI NOBORU; MATSUYAMA SHIN; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 42, Suppl.1, 264, 264, Jun. 2011
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の肝カルチノイド4症例におけるソナゾイド造影超音波検査所見
  NAKAMURA KENSUKE; HOSOYA KENJI; YAMASAKI MASAHIRO; OTA HIROSHI; YEE LIM SUE; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 42, Suppl.1, 261, 261, Jun. 2011
  (一社)日本獣医麻酔外科学会, Japanese
• EFFECTS OF SILDENAFIL CITRATE ON DOGS WITH EISENMENGER'S SYNDROME
  K. Nakamura; M. Yamasaki; H. Ohta; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 25, 3, 660, 660, May 2011
  English, Summary international conference
• パルス超音波と微小気泡によるシスプラチンの抗腫瘍効果増強の基礎的知見
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 38, 3, 338, 338, May 2011
  (公社)日本超音波医学会, Japanese
• “診る目を養う”超音波検査 第5回 門脈シャントを超音波で診断する
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, SA Med, 13, 2, 86, 90, 01 Apr. 2011
  (株)エデュワードプレス, Japanese
• 診断用超音波とマイクロバブルによるドラッグデリバリーのin vitroでの最適化
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BADULA; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 38, Suppl., S316, S316, Apr. 2011
  (公社)日本超音波医学会, Japanese
• シトシンアラビノシドにより良好に維持されている特発性脳炎の犬の2例
  OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医皮膚科学会学術大会・総会, 14th, Vol.1, 117, 11 Mar. 2011
  Japanese
• 二次性赤血球増加症を伴ったEisenmenger症候群の犬に対するシルデナフィルの有効性の検討
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医皮膚科学会学術大会・総会, 14th, Vol.1, 175, 11 Mar. 2011
  Japanese
• 内分泌疾患の画像診断
  TAKIGUCHI MITSUYOSHI, 日本獣医皮膚科学会学術大会・総会, 14th, Vol.2, 272, 11 Mar. 2011
  Japanese
• 超音波とマイクロバブルによるドラッグデリバリーに薬剤濃度とバブル濃度が及ぼす影響
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BANDULA; TAMURA YU; YEE LIM SUE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医皮膚科学会学術大会・総会, 14th, Vol.1, 183, 11 Mar. 2011
  Japanese
• アムホテリシンBによるイヌバベシア症の治療に関する研究
  YAMASAKI MASAHIRO; O KOJAKU; BANDULA KUMARA W. R; TANIYAMA YUSUKE; TSUBOI YOSHIHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 151回, 216, 216, Mar. 2011
  (公社)日本獣医学会, Japanese
• 甲状腺の外科(外科適応となる)甲状腺疾患の病態と診断
  TAKIGUCHI MITSUYOSHI, Surgeon, 15, 2, 8, 17, 01 Mar. 2011
  (株)エデュワードプレス, Japanese
• 第二世代超音波造影剤ソナゾイドを用いた造影超音波検査による犬の肝臓および脾臓腫瘤の診断に関する研究
  NAKAMURA KENSUKE; TAKAGI SATOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; TAMURA YU; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 151回, 82, 82, Mar. 2011
  (公社)日本獣医学会, Japanese
• アムホテリシンBのin vivoにおける抗バベシア効果に関する研究
  YAMASAKI MASAHIRO; HWANG SHIANG-JYI; KUMARA W.R.BANDULA; TANIYAMA YUSUKE; TSUBOI YOSHIHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本寄生虫学会大会プログラム・抄録集, 80th, 73, Feb. 2011
  Japanese
• “診る目を養う”超音波検査 第4回 肝臓腫瘤を超音波で鑑別する
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, SA Med, 13, 1, 101, 107, 01 Feb. 2011
  (株)エデュワードプレス, Japanese
• シトシンアラビノシドが奏効した肉芽腫性髄膜脳脊髄炎疑いの犬の1例
  OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 55, 1, 9, 11, Jan. 2011
  (公社)北海道獣医師会, Japanese
• “診る目を養う”超音波検査 第3回 黄疸を超音波で鑑別する(2)肝実質の超音波検査
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, SA Med, 12, 6, 65, 69, 01 Dec. 2010
  (株)エデュワードプレス, Japanese
• 急性膵炎とどう戦う―急性膵炎の病態生理と診断―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 31st, 3, 152, 153, 19 Nov. 2010
  動物臨床医学会, Japanese
• 腹部エコーのブラッシュ・アップ―上腹部を極める―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 31st, 1, 1, 1, 19 Nov. 2010
  動物臨床医学会, Japanese
• 消化器疾患の内科学的アプローチ―診断技術の向上と的確な治療を目指して―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 31st, 3, 187, 187, 19 Nov. 2010
  動物臨床医学会, Japanese
• ガストリン産生性カルチノイドに対し徐放性オクトレオチド製剤を使用した犬の1例
  細谷 謙次; 金子 一成; 滝口 満喜; 中村 健介; 高木 哲; 奥村 正裕, 動物臨床医学会年次大会プロシーディング, 31回, 2, 193, 194, Nov. 2010
  動物臨床医学会, Japanese
• “診る目を養う”超音波検査 第2回 黄疸を超音波で鑑別する(1)胆道系の超音波検査
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE, SA Med, 12, 5, 79, 85, 01 Oct. 2010
  (株)エデュワードプレス, Japanese
• 教育の質保証と獣医学モデル・コア・カリキュラムおよび獣医学共用試験の役割 「eラーニングコンテンツ作成の試み」内科学
  滝口 満喜, 日本獣医学会学術集会講演要旨集, 150回, 131, 131, Sep. 2010
  (公社)日本獣医学会, Japanese
• 微小気泡存在下での診断用超音波照射によるシスプラチンの抗腫瘍効果増強の検討
  SASAKI AZUMA; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; BANDULA KUMARA; TAMURA YU; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 150回, 297, 297, Sep. 2010
  (公社)日本獣医学会, Japanese
• 犬のガストリノーマに対してサンドスタチンLARを投与した1例
  金子 一成; 細谷 謙次; 滝口 満喜; 中村 健介; 北村 二郎; 高木 哲; 奥村 正裕, 北海道獣医師会雑誌, 54, 8, 450, 450, Aug. 2010
  (公社)北海道獣医師会, Japanese
• “診る目を養う”超音波検査 第1回 腹部エコー:基本と上達のコツ
  TAKIGUCHI MITSUYOSHI, SA Med, 12, 4, 67, 71, 01 Aug. 2010
  (株)エデュワードプレス, Japanese
• 造影超音波検査により病変の特定が可能であったインスリノーマの犬2例
  NAKAMURA KENSUKE; TAKAGI SATOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 460, 460, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 胆嚢粘液嚢腫の犬19症例の回顧的解析
  MURATA NAOMITSU; TAKAGI SATOSHI; HOSOYA KENJI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI; SATO KOTA; OKUMURA MASAHIRO, 北海道獣医師会雑誌, 54, 8, 462, 462, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 細菌性心内膜炎の犬の1例
  MORI SHINSUKE; OTA HIROSHI; SAKAI HIDEFUMI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 459, 459, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 犬の皮膚無菌性肉芽腫/化膿性肉芽腫症候群と診断した1症例
  WAKAKI NOZOMI; YAMASAKI MASAHIRO; OTA HIROSHI; NASUNO TOYOHIKO; OCHIAI KENJI; TAKEBAYASHI KENSAKU; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 464, 464, Aug. 2010
  (公社)北海道獣医師会, Japanese
• リンパ管拡張症を疑う胸水貯留に続発して肺葉捻転を起こした犬の1例
  MURAKAMI MASAHIRO; NAKAMURA KENSUKE; SASAKI AZUMA; OTA HIROSHI; YAMASAKI MASAHIRO; TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 464, 464, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 造影3DCTにより確定診断し得た犬の重複大動脈弓の1例
  MATSUYAMA SHIN; KANEKO KAZUNARI; YAMANE YOSHIHISA; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 458, 458, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 心房中隔欠損症と僧帽弁閉鎖不全症を併発した犬の1例
  OGI NAOKO; NAKAMURA KENSUKE; MATSUYAMA SHIN; HASHIBA MINORU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 458, 458, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 経皮的エコーガイド下胆嚢穿刺により確定診断した細菌性胆嚢炎の犬と猫の2例
  UKAI AYAKO; KANEKO KAZUNARI; OKAWA MINAKO; OTA HIROSHI; KOBAYASHI HIDEAKI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 54, 8, 461, 461, Aug. 2010
  (公社)北海道獣医師会, Japanese
• 二次性赤血球増加症を伴ったEisenmenger症候群の犬に対するシルデナフィルの有効性の検討
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 41, Suppl.1, 294, 294, Jul. 2010
  (一社)日本獣医麻酔外科学会, Japanese
• CONTRAST-ENHANCED ULTRASONOGRAPHY WITH SONAZOID (R) FOR CHARACTERIZATION OF FOCAL SPLENIC LESIONS
  K. Nakamura; H. Ohta; M. Yamasaki; M. Takiguchi, JOURNAL OF VETERINARY INTERNAL MEDICINE, 24, 3, 733, 734, May 2010
  English, Summary international conference
• 培養Babesia gibsoniの末梢血単核球への侵入と増殖の検討
  TANIYAMA YUSUKE; YAMASAKI MASAHIRO; HWANG SHIANG-JYI; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本寄生虫学会大会プログラム・抄録集, 79th, 70, May 2010
  Japanese
• ビジュアルで学ぶ!内視鏡検査と治療 第7回 手技編 下部消化管の内視鏡検査
  TAKIGUCHI MITSUYOSHI, MVM, 19, 4, 68, 69, 01 May 2010
  (株)ファームプレス, Japanese
• 超音波診断装置と微小気泡によるシスプラチンの抗腫瘍効果の増強
  SASAKI NOBORU; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; KUMARA BANDULA; OTA HIROSHI; YAMASAKI MASAHIRO; KUDO NOBUKI; TAKIGUCHI MITSUYOSHI, 超音波医学, 37, Suppl., S322, S322, Apr. 2010
  (公社)日本超音波医学会, Japanese
• Diminazene aceturate耐性Babesia gibsoni株に対する他の抗バベシア原虫薬の効果
  黄 香寂; 谷山 裕介; 山崎 真大; 大田 寛; 滝口 満喜, 獣医寄生虫学会誌, 8, 2, 149, 149, Mar. 2010
  日本獣医寄生虫学会, Japanese
• イヌおよびネコの腎疾患における病態差―腎機能とスリット膜関連分子の動態―
  ICHII OSAMU; YABUKI AKIRA; SASAKI NOBUYA; OTA HIROSHI; TAKIGUCHI MITSUYOSHI; OTSUKA SAORI; HASHIMOTO YOSHIHARU; ENDO DAIJI; KON YASUHIRO, 日本獣医学会学術集会講演要旨集, 149回, 213, 213, Mar. 2010
  (公社)日本獣医学会, Japanese
• 犬の肝臓腫瘤30例に対するソナゾイド造影超音波検査
  NAKAMURA KENSUKE; TAKAGI SATOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; SASAKI NOBORU; TAKIGUCHI MITSUYOSHI, 日本獣医師会三学会年次大会講演要旨集, 2009, 237, 07 Jan. 2010
  Japanese
• 診断と内科治療
  TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 40, Supplement 2, 99, 99, 10 Dec. 2009
  (一社)日本獣医麻酔外科学会, Japanese
• 外科手術を実施した犬の肝細胞癌17症例の検討
  TAKAGI SATOSHI; TAKIGUCHI MITSUYOSHI; YAMASAKI MASAHIRO; OTA HIROSHI; NAKAMURA KENSUKE; OSAKI TOMOHIRO; HOSOYA YOSHITSUGU; OKUMURA MASAHIRO, 獣医麻酔外科学雑誌, 40, Suppl.2, 196, 196, Dec. 2009
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の消化器疾患の内科学的アプローチ―最新の診断技術と治療法の紹介―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 30th, 3, 247, 247, 20 Nov. 2009
  動物臨床医学会, Japanese
• 腹部エコー:基本と上達への道
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 30th, 1, 9, 9, 20 Nov. 2009
  動物臨床医学会, Japanese
• 犬のリンパ球プラズマ細胞性腸炎における細胞接着分子クローディンの発現変動
  OTA HIROSHI; MURAKAMI MASAHIRO; KUMARA BANDURA; SASAKI NOBORU; NAKAMURA KENSUKE; KO KOJAKU; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 148回, 261, 261, Sep. 2009
  (公社)日本獣医学会, Japanese
• Diminazene aceturate耐性Babesia gibsoni株に対する他の抗バベシア原虫薬の効果
  KO KOJAKU; TANIYAMA YUSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 148回, 188, 188, Sep. 2009
  (公社)日本獣医学会, Japanese
• 犬の脾臓腫瘤におけるソナゾイド造影超音波検査の有用性
  NAKAMURA KENSUKE; YAMASAKI MASAHIRO; OTA HIROSHI; SASAKI NOBORU; MURAKAMI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 148回, 268, 268, Sep. 2009
  (公社)日本獣医学会, Japanese
• 犬の肝臓腫瘤28結節に対するソナゾイド造影超音波検査
  NAKAMURA KENSUKE; TAKAGI SATOSHI; SASAKI AKIRA; MURAKAMI MASAHIRO; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 101, 101, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 胆嚢粘液嚢腫の犬5例における周術期管理
  MITSUDA KENTA; TAKAGI SATOSHI; SUNAGA TAKAFUMI; TAKIGUCHI MITSUYOSHI; OTA HIROSHI; SATO KOTA; OSAKI TOMOHIRO; OKUMURA MASAHIRO, 北海道獣医師会雑誌, 53, 8, 93, 93, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 大脳辺縁系の壊死が疑われた猫の1症例
  KAMIJO HIROMI; OTA HIROSHI; MATSUMOTO MASANORI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 103, 103, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 筋炎の併発が疑われた劇症型重症筋無力症の犬の1例
  UKAI AYAKO; YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; NAKAO KIICHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 98, 98, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 消化器系の診療手技 消化管の超音波検査
  TAKIGUCHI MITSUYOSHI, SA Med, 11, 4, 24, 33, 01 Aug. 2009
  (株)エデュワードプレス, Japanese
• 本態性高Na血症が疑われた神経症状を示した犬と猫の2例
  MURAKAMI MASAHIRO; OTA HIROSHI; NAKAMURA KENSUKE; SASAKI AKIRA; YAMASAKI MASAHIRO; ETO MASAYUKI; KOSEKI YUMI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 97, 97, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 気管支内視鏡検査で気管支狭窄を認めた慢性発咳の犬の1症例
  SASAKI AKIRA; OTA HIROSHI; ANDO REMI; NAKAMURA KENSUKE; MURAKAMI MASAHIRO; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 88, 88, Aug. 2009
  (公社)北海道獣医師会, Japanese
• ボストンテリアの脾臓捻転の1症例
  OTA HIROSHI; TAKAGI SATOSHI; NAKAZAWA SHIGEKI; MURAKAMI MASAHIRO; SASAKI AKIRA; NAKAMURA KENSUKE; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 53, 8, 89, 89, Aug. 2009
  (公社)北海道獣医師会, Japanese
• 腹部エコーの基礎とブラッシュ・アップ
  TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 40, Supplement 1, 150, 150, 20 Jun. 2009
  (一社)日本獣医麻酔外科学会, Japanese
• 腎疾患の新しい診断指標―Interleukin 1 family,member 6は尿細管傷害マーカーになり得る―
  ICHII OSAMU; KON YASUHIRO; SASAKI NOBUYA; HASHIMOTO YOSHIHARU; OTA HIROSHI; TAKIGUCHI MITSUYOSHI; YABUKI AKIRA; ENDO DAIJI, 日本獣医学会学術集会講演要旨集, 147回, 79, 79, Mar. 2009
  (公社)日本獣医学会, Japanese
• 培養Babesia gibsoniのストレス環境下におけるheat shock protein 70の役割に関する研究
  YAMASAKI MASAHIRO; HWANG SHIANG-JYI; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本寄生虫学会大会プログラム・抄録集, 78th, 86, Feb. 2009
  Japanese
• SLEが疑われた血球貪食症候群の猫の1例
  YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; SASAKI AZUMA; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医師会三学会年次大会講演要旨集, 2008, 296, 25 Dec. 2008
  Japanese
• ポリエン系抗生物質アムホテリシンBの抗Babesia gibsoni効果
  原田 絵梨子; 山崎 真大; 黄 寂香; 大田 寛; 滝口 満喜, 獣医寄生虫学会誌, 7, 2, 90, 90, Dec. 2008
  日本獣医寄生虫学会, Japanese
• Diminazene aceturate耐性Babesia gibsoni株の作成
  黄 香寂; 山崎 真大; 大田 寛; 滝口 満喜, 獣医寄生虫学会誌, 7, 2, 92, 92, Dec. 2008
  日本獣医寄生虫学会, Japanese
• 初心者のための基本的な消化管の画像診断(X線,超音波,内視鏡)
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 29th, 1, 110, 110, 14 Nov. 2008
  動物臨床医学会, Japanese
• 簡便な犬リポ蛋白質の測定法の開発と臨床例への応用
  TAKASAKI YUKARI; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 29回, 3, 37, 40, Nov. 2008
  動物臨床医学会, Japanese
• 超音波検査により求めた正常なビーグル犬の甲状腺体積と体重の相関
  SASAKI NOBORU; NAKAMURA KENSUKE; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 29回, 3, 29, 32, Nov. 2008
  動物臨床医学会, Japanese
• 犬の肝臓における造影超音波検査の有用性の検討
  NAKAMURA KENSUKE; SASAKI HIGASHI; YOSHIKAWA MUNEYOSHI; O KOJAKU; OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 146回, 265, 265, Sep. 2008
  (公社)日本獣医学会, Japanese
• 犬の消化菅粘膜におけるクローディン分子群の発現:十二指腸を中心として
  YAMAGUCHI TOMOO; OTA HIROSHI; NAKAMURA KENSUKE; O KOJAKU; SASAKI HIGASHI; YOSHIKAWA MUNEYOSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 146回, 258, 258, Sep. 2008
  (公社)日本獣医学会, Japanese
• Diminazene aceturate耐性Babesia gibsoni株の作成
  O KOJAKU; YAMASAKI MASAHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 146回, 173, 173, Sep. 2008
  (公社)日本獣医学会, Japanese
• ポリエン系抗生物質アムホテリシンBの抗Babesia gibsoni効果
  HARADA ERIKO; YAMASAKI MASAHIRO; O KOJAKU; OTA HIROSHI; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 146回, 172, 172, Sep. 2008
  (公社)日本獣医学会, Japanese
• 抗リン脂質抗体症候群が疑われた不明熱のミニチュア・ダックスフンドの1例
  NAKAMURA KENSUKE; SASAKI NOBORU; YOSHIKAWA MUNEYOSHI; OTA HIROSHI; YAMAZAKI MASAHIRO; OBA MASAYO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 8, 410, 410, Aug. 2008
  (公社)北海道獣医師会, Japanese
• 犬の無菌性好酸球性膿疱症と診断した2症例
  MINAMIYAMA ERI; YAMAZAKI MASAHIRO; OTA HIROSHI; HONDA HIDETAKA; NASUNO TOYOHIKO; OCHIAI KENJI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 8, 413, 413, Aug. 2008
  (公社)北海道獣医師会, Japanese
• 気胸を併発した先天性肺葉性肺気腫の犬の1例
  KAMIJO HIROMI; TAKAGI SATOSHI; OTA HIROSHI; YAMAZAKI MASAHIRO; KIMURA TAKASHI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 8, 420, 420, Aug. 2008
  (公社)北海道獣医師会, Japanese
• エリスロポエチン依存性の二次性多血症が疑われた腎リンパ腫の犬の1例
  SASAKI AZUMA; YOSHIKAWA MUNEYOSHI; NAKAMURA KENSUKE; OTA HIROSHI; YAMAZAKI MASAHIRO; SATO KOTA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 8, 415, 415, Aug. 2008
  (公社)北海道獣医師会, Japanese
• SLEが疑われた血球貪食症候群の猫の1例
  YOSHIKAWA MUNEYOSHI; SHODA MASATO; NAKAMURA KENSUKE; SASAKI NOBORU; OTA HIROSHI; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 8, 410, 410, Aug. 2008
  (公社)北海道獣医師会, Japanese
• 犬の炎症性腸疾患:リンパ球形質細胞性腸炎・結腸炎を中心に
  OTA HIROSHI; YAMASAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 52, 7, 263, 265, Jul. 2008
  (公社)北海道獣医師会, Japanese
• 第二世代超音波造影剤ソナゾイドによる肝臓腫瘤の診断
  NAKAMURA KENSUKE; OTA HIROSHI; YAMAZAKI MASAHIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 39, Suppl.1, 227, 227, Jun. 2008
  (一社)日本獣医麻酔外科学会, Japanese
• 腹部エコー検査のススメ―小動物臨床における超音波造影剤の可能性
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, CAP (Companion Anim Pract), 23, 225, 23, 28, 01 Mar. 2008
  (株)緑書房, Japanese
• クエン酸シルデナフィルが奏功した逆行性動脈管開存症のイヌの2例
  NAKAMURA KENSUKE; MORISHITA KEITARO; NAKADE TETSUYA; TAKIGUCHI MITSUYOSHI, 日本獣医師会三学会年次大会講演要旨集, 2007, 245, 2008
  Japanese
• 第二世代超音波造影剤ソナゾイドの小動物臨床への応用に関する基礎的検討
  TAKIGUCHI MITSUYOSHI; NAKAMURA KENSUKE, 獣医麻酔外科学雑誌, 38, Supplement 2, 290, 291, 20 Nov. 2007
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の膵疾患における超音波ヒストグラム解析の有用性に関する基礎的研究
  NAKAMURA KENSUKE; MORISHITA KEITARO; TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 144th, 160, 160, 27 Aug. 2007
  (公社)日本獣医学会, Japanese
• クエン酸シルデナフィルが奏功した逆行性動脈管開存症の犬の2例
  NAKAMURA KENSUKE; MORISHITA KEITARO; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 51, 8, 333, 333, 01 Aug. 2007
  (公社)北海道獣医師会, Japanese
• メソトレキセートが奏功したステロイド抵抗性炎症性腸疾患の犬の1例
  MORISHITA KEITARO; NAKAMURA KENSUKE; HIRAYAMA KAZUKO; TANIYAMA HIROYUKI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 51, 8, 335, 335, 01 Aug. 2007
  (公社)北海道獣医師会, Japanese
• デジタルX線(DR)撮影下における非選択的血管造影法により逆行性動脈管開存症と診断された犬の2例
  MORISHITA KEITARO; NAKAMURA KENSUKE; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 日本獣医循環器学会抄録, 86th, Suppl.1, 203, 203, 2007
  (一社)日本獣医麻酔外科学会, Japanese
• 十二指腸に閉塞性病変を伴った炎症性陽疾患の犬の1例
  中村 健介; 森下 啓太郎; 廉澤 剛; 平山 和子; 谷山 弘行; 滝口 満喜, 北海道獣医師会雑誌, 50, 8, 79, 79, Aug. 2006
  (公社)北海道獣医師会, Japanese
• I型偽性低アルドステロン症が疑われた犬の1例
  MORISHITA KEITARO; NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 336, 80, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• 犬の慢性リンパ性白血病:メルファラン継続投与による良好な長期コントロールの1例
  HIROSE TAKESHI; NAKAMURA KENSUKE; MORISHITA KEITARO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 346, 90, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• デジタルX線(DR)撮影下における非選択的血管造影法により逆行性動脈管開存症と診断された犬の1例
  TOOYAMA NOBUO; NAKAMURA KENSUKE; MORISHITA KEITARO; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 355, 99, 01 Aug. 2006
  (公社)北海道獣医師会, Japanese
• 十二指腸に閉塞性病変を伴った炎症性腸疾患の犬の1例
  NAKAMURA KENSUKE; MORISHITA KEITARO; RENZAWA TSUYOSHI; HIRAYAMA KAZUKO; TANIYAMA HIROYUKI; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 50, 8, 335, 01 Aug. 2006
  Japanese
• 肉芽腫性胃炎により幽門狭窄症を呈したイヌの一例
  NAKAMURA KENSUKE; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 37, Supplement 1, 207, 207, 20 Jun. 2006
  (一社)日本獣医麻酔外科学会, Japanese
• 壊死性髄膜脳炎(NME)が強く疑われた犬8例と抗アストロサイト抗体
  NAKADE TETSUYA; MATSUKI NAOAKI; INOUE AIKA; TAKIGUCHI MITSUYOSHI; NIIYAMA MASAYOSHI; IZUMISAWA YASUHARU; TANADA ATSUSHI; YOSHIOKA YU; YOSHIOKA HINATSU; WATANABE TAKUO; TERASAWA ASAKO; ENOMOTO KOICHI; ISHIZUKA KISEKO; TAMAI SATOSHI, 動物臨床医学会年次大会プロシーディング, 26th, 2, 195, 196, 18 Nov. 2005
  動物臨床医学会, Japanese
• 臨床的に線維軟骨性塞栓症と診断されたイヌのMRI所見
  MIYOSHI KENJIRO; NAKADE TETSUYA; IZUMISAWA YASUHARU; TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 26th, 3, 26, 29, 18 Nov. 2005
  動物臨床医学会, Japanese
• 食道狭窄:とくに猫,バルーンなどを使った治療
  TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 36, Supplement 2, 73, 73, 01 Oct. 2005
  (一社)日本獣医循環器学会, Japanese
• 私立および国立獣医大学における臨床講座の違いとそれぞれの教育・研究のあり方
  TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 140th, 30, 30, 31 Aug. 2005
  (公社)日本獣医学会, Japanese
• 犬の明らかな血小板増多を伴った赤芽球癆
  町田 竜彦; 内田 英二; 伊藤 真之; 木村 真; 滝口 満喜; 谷山 弘行, 北海道獣医師会雑誌, 49, 8, 71, 71, Aug. 2005
  (公社)北海道獣医師会, Japanese
• 眼底出血から副腎腫瘍が疑われた犬の1例
  若生 晋輔; 滝口 満喜; 前原 誠也; 久保 明; 都築 圭子; 伊藤 典彦; 泉澤 康晴, 北海道獣医師会雑誌, 49, 8, 73, 73, Aug. 2005
  (公社)北海道獣医師会, Japanese
• 犬猫の消化管内異物の超音波診断
  TAKIGUCHI MITSUYOSHI, 獣医画像診断, 16/17, 3, 8, Aug. 2005
  Japanese
• 眼底出血から副腎腫ようが疑われた犬の1例
  WAKO SHINSUKE; TAKIGUCHI MITSUYOSHI; MAEHARA MASAYA; KUBO AKIRA; TSUZUKI KEIKO; ITO NORIHIKO; IZUMISAWA YASUHARU, 北海道獣医師会雑誌, 49, 8, 289, 01 Aug. 2005
  Japanese
• 頻回の内視鏡下バルーン拡張術が功を奏した食道狭窄の猫の1例
  MIWA ERIKO; MIYATA KAYOKO; MACHIDA TATSUHIKO; NAKAMURA KENSUKE; NAKAIDE TETSUYA; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 49, 8, 276, 60, 01 Aug. 2005
  (公社)北海道獣医師会, Japanese
• 犬の明らかな血小板増多を伴った赤芽球ろう
  MACHIDA TATSUHIKO; UCHIDA EIJI; ITO MASAYUKI; KIMURA MAKOTO; TAKIGUCHI MITSUYOSHI; TANIYAMA HIROYUKI, 北海道獣医師会雑誌, 49, 8, 287, 01 Aug. 2005
  Japanese
• 超音波検査により粥状動脈硬化症と診断された犬の1例
  NAKAMURA KENSUKE; KITAMURA JIRO; TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 49, 8, 293, 77, 01 Aug. 2005
  (公社)北海道獣医師会, Japanese
• 最近の腹部超音波診断の有効性
  滝口 満喜, 日本獣医循環器学会抄録, 82, 127, 25 Jun. 2005
  Japanese
• 血管超音波検査によりアテローム性動脈硬化症が疑われた犬の一例
  中村 健介; 北村 二郎; 滝口 満喜, 日本獣医循環器学会抄録, 82, 249, 249, 25 Jun. 2005
  Japanese
• 最近の腹部超音波診断の有効性
  TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 36, Supplement 1, 120, 120, 20 Jun. 2005
  (一社)日本獣医麻酔外科学会, Japanese
• 血管超音波検査によりアテローム性動脈硬化症が疑われた犬の一例
  NAKAMURA KENSUKE; KITAMURA JIRO; TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 36, Supplement 1, 240, 240, 20 Jun. 2005
  (一社)日本獣医麻酔外科学会, Japanese
• Autoimmunity against a tissue kallikrein in IQI/Jic mice - A model for Sjogren's syndrome
  K Takada; M Takiguchi; A Konno; A Inaba, JOURNAL OF BIOLOGICAL CHEMISTRY, 280, 5, 3982, 3988, Feb. 2005
  English
• 紐状異物による十二指腸の穿孔に対して腸管内側から修復を行った犬の1例
  HOSHINO YUKI; KADOSAWA TSUYOSHI; TAKIGUCHI MITSUYOSHI; OKUMURA MASAHIRO; FUJINAGA TOORU, 獣医麻酔外科学雑誌, 36, 1, 19, 22, 31 Jan. 2005
  (一社)日本獣医麻酔外科学会, Japanese
• 犬のポリープ様ぼうこう炎の超音波画像所見
  TAKIGUCHI MITSUYOSHI, 日本産業動物獣医学会・日本小動物獣医学会・日本獣医公衆衛生学会年次大会, 2004, 295, 2005
  Japanese
• 胸部,腹部の超音波診断法
  TAKIGUCHI MITSUYOSHI, 日本産業動物獣医学会・日本小動物獣医学会・日本獣医公衆衛生学会年次大会, 2004, 212, 215, 2005
  Japanese
• 腎臓の障害をいかにして知るか―腎疾患の画像診断―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 25th, 1, 326, 326, 19 Nov. 2004
  動物臨床医学会, Japanese
• 犬と猫の急性すい炎のすべてがわかるセミナー―急性すい炎の画像診断―
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 25th, 3, 240, 241, 19 Nov. 2004
  動物臨床医学会, Japanese
• 脳溢血と診断した小型初老犬の1例
  天内 満雄; 滝口 満喜; 黒田 敏, 北海道獣医師会雑誌, 48, 10, 4, 6, Oct. 2004
  (公社)北海道獣医師会, Japanese
• IVRによりコイル栓塞術を実施したイヌ動脈管開存症の1例
  NAKADE TETSUYA; RENZAWA TAKESHI; TAKIGUCHI MITSUYOSHI; ASANO KAZUYUKI, 獣医麻酔外科学雑誌, 35, Supplement 2, 61, 61, 01 Oct. 2004
  (一社)日本獣医麻酔外科学会, Japanese
• 脳溢血と診断した小型初老犬の1例
  AMAUCHI MITSUO; TAKIGUCHI MITSUYOSHI; KURODA SATOSHI, 北海道獣医師会雑誌, 48, 10, 422, 424, 01 Oct. 2004
  Japanese
• リパーゼ産生性すい臓腫ようによる無菌性脂肪織炎が疑われた犬の1例
  SEKIGUCHI MICHIKO; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 北海道獣医師会雑誌, 48, 8, 305, 305, 01 Aug. 2004
  (公社)北海道獣医師会, Japanese
• ホジキン型リンパ腫が疑われた猫の1例
  OKAZAKI KAZUKO; TAKIGUCHI MITSUYOSHI; KIMURA TAKAFUMI; MATSUDA EIICHIRO; INABA MUTSUMI, 北海道獣医師会雑誌, 48, 8, 319, 319, 01 Aug. 2004
  (公社)北海道獣医師会, Japanese
• 新生子牛のグロビンスイッチングにおける転写因子erythroid Kruppel‐like factorの役割
  ARASHIKI NOBUTO; ITO DAISUKE; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 138th, 148, 148, 01 Aug. 2004
  (公社)日本獣医学会, Japanese
• 反すう動物赤血球膜タンパク質gp155の分子実態はMRP4である
  HIRAI MAYUMI; ITO DAISUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 138回, 178, 178, Aug. 2004
  (公社)日本獣医学会, Japanese
• クローディン‐16欠損胎子腎の組織構築とタイトジャンクション構成分子の発現動態
  OTA HIROSHI; ITO DAISUKE; KAGEYAMA SOICHI; HIRAYAMA HIROKI; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 138回, 148, 148, Aug. 2004
  (公社)日本獣医学会, Japanese
• 血尿を主訴とした雌ウサギの子宮疾患例
  NAGANO MASASHI; KATAGIRI SEIJI; TAKIGUCHI MITSUYOSHI; KOMATSU TOMOHIKO; NAGASHIMA TARO; IKAWA SHIN'ICHI; TAKAHASHI YOSHIYUKI, 北海道獣医師会雑誌, 48, 8, 318, 318, 01 Aug. 2004
  (公社)北海道獣医師会, Japanese
• 犬のポリープ様ぼうこう炎の超音波画像
  TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 北海道獣医師会雑誌, 48, 8, 307, 307, 01 Aug. 2004
  (公社)北海道獣医師会, Japanese
• シェーグレン症候群モデルマウス・IQI/Jicにおける自己抗原の同定
  TAKADA KENSUKE; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 138th, 160, 160, 01 Aug. 2004
  (公社)日本獣医学会, Japanese
• バンド3の細胞膜移行におけるC末端細胞質領域の重要性
  ITO DAISUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 138回, 178, 178, Aug. 2004
  (公社)日本獣医学会, Japanese
• Spontaneous development of multiple glandular and extraglandular lesions in aged IQI/Jic mice: a model for primary Sjogren's syndrome
  K Takada; M Takiguchi; A Konno; M Inaba, RHEUMATOLOGY, 43, 7, 858, 862, Jul. 2004
  English
• Spontaneous development of multiple glandular and extraglandular lesions in aged IQI/Jic mice: a model for primary Sjogren's syndrome
  K Takada; M Takiguchi; A Konno; M Inaba, RHEUMATOLOGY, 43, 7, 858, 862, Jul. 2004
  English
• 乳腺上皮細胞におけるタイトジャンクション構成タンパク質の発現動態
  OTA HIROSHI; KOMABAYASHI KEN'ICHI; ITO DAISUKE; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本膜学会年会講演要旨集, 26th, 56, 30 Apr. 2004
  Japanese
• 牛遺伝性バンド3欠損症ホモ接合型胎子における組織病態
  ITO DAISUKE; KAGEYAMA SOICHI; SAWAI KEN; NAGATA AKIHIRO; OTA HIROSHI; TAKIGUCHI MITSUYOSHI; BAN AKIRA; WATANABE DAISAKU; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 137回, 136, 136, Mar. 2004
  (公社)日本獣医学会, Japanese
• 乳腺管腔上皮におけるタイトジャンクション構成蛋白質の発現動態
  OTA HIROSHI; KOMABAYASHI KEN'ICHI; ITO DAISUKE; WATANABE ATSUSHI; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 137回, 152, 152, Mar. 2004
  (公社)日本獣医学会, Japanese
• Autoimmunity against a tissue kallikrein in IQI/Jic mice, a model for Sjogren's syndrome 'jointly worked'
  J. Biol. Chem.., (in press), 2004
• Diagnostic ultrasound of polypoid cystitis in the dog 'jointly worked'
  J. Vet. Med. Sci., (in press), 2004
• Diagnostic ultrasound of polypoid cystitis in the dog 'jointly worked'
  J. Vet. Med. Sci., (in press), 2004
• 腎疾患の新たな診断と治療法を目指して 腎疾患の画像診断
  TAKIGUCHI MITSUYOSHI, 獣医畜産新報, 57, 990, 35, 40, 01 Jan. 2004
  文永堂出版(株), Japanese
• Magnetic resonance imaging and immunoblot analyses in rats with experimentally induced cerebral alveolar echinococcosis
  T Asanuma; Y Matsumoto; M Takiguchi; O Inanami; M Nakao; K Nakaya; A Ito; A Hashimoto; M Kuwabara, COMPARATIVE MEDICINE, 53, 6, 649, 656, Dec. 2003
  English
• Feline leptin: Immunogenic and biological activities of the recombinant protein, and its measurement by ELISA
  H Shibata; N Sasaki; T Honjoh; Ohishi, I; M Takiguchi; K Ishioka; M Ahmed; M Soliman; K Kimura; M Saito, JOURNAL OF VETERINARY MEDICAL SCIENCE, 65, 11, 1207, 1211, Nov. 2003
  English
• Feline leptin: Immunogenic and biological activities of the recombinant protein, and its measurement by ELISA
  H Shibata; N Sasaki; T Honjoh; Ohishi, I; M Takiguchi; K Ishioka; M Ahmed; M Soliman; K Kimura; M Saito, JOURNAL OF VETERINARY MEDICAL SCIENCE, 65, 11, 1207, 1211, Nov. 2003
  English
• 紐状異物による十二指腸の線状穿孔に対して腸管内側から修復を行った犬の一例
  HOSHINO YUKI; KADOSAWA TAKESHI; TAKIGUCHI MITSUYOSHI; MATSUHISA YUKI; OKUMURA MASAHIRO; FUJINAGA TOORU, 獣医麻酔外科学雑誌, 34, Supplement 2, 99, 100, 20 Sep. 2003
  (一社)日本獣医麻酔外科学会, Japanese
• 調節帯異常により左室機能障害を呈した猫の超音波検査
  HAMAMOTO KOHEI; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 136th, 205, 205, 10 Sep. 2003
  (公社)日本獣医学会, Japanese
• シェーグレン症候群モデルマウス・IQI/Jicにおける多発性自己免疫病変の組織学的解析および自己抗原の同定
  TAKADA KENSUKE; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 136th, 218, 218, 10 Sep. 2003
  (公社)日本獣医学会, Japanese
• 副腎性性ホルモン機能こう進症とポメラニアンの脱毛症X
  TAKIGUCHI MITSUYOSHI; TAKADA KENSUKE, 日本獣医学会学術集会講演要旨集, 136th, 54, 54, 10 Sep. 2003
  (公社)日本獣医学会, Japanese
• 腎疾患の画像診断
  TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 136th, 50, 50, 10 Sep. 2003
  (公社)日本獣医学会, Japanese
• 牛腎尿細管においてクローディン16と共存するパートナークローディンの解析と遺伝子の単離
  OTA HIROSHI; ITO DAISUKE; KOMABAYASHI KEN'ICHI; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 136回, 196, 196, Sep. 2003
  (公社)日本獣医学会, Japanese
• バンド3欠損症変異バンド3認識・分解の分子機構―R664X変異周辺領域認識の検証―
  ITO DAISUKE; OTA HIROSHI; TAKIGUCHI MITSUYOSHI; INABA MUTSUMI, 日本獣医学会学術集会講演要旨集, 136回, 217, 217, Sep. 2003
  (公社)日本獣医学会, Japanese
• 右大動脈弓遺残と動脈管開存が併発した犬の1例
  KAWAMURA TAKAO; TAKIGUCHI MITSUYOSHI; HAMAMOTO KOHEI; HASEBE RIE; RENZAWA TAKESHI; INABA MUTSUMI, 北海道獣医師会雑誌, 47, 8, 316, 316, 01 Aug. 2003
  (公社)北海道獣医師会, Japanese
• 甲状腺ホルモン製剤補充療法により病態が改善された拡張型心筋症の犬の1例
  OKAZAKI KAZUKO; TAKIGUCHI MITSUYOSHI; HAMAMOTO KOHEI; MURAOKA NOBUYOSHI; INABA ATSUSHI, 北海道獣医師会雑誌, 47, 8, 295, 295, 01 Aug. 2003
  (公社)北海道獣医師会, Japanese
• 超音波検査によりしん出性収縮性心膜炎と診断された犬の1例
  HAMAMOTO KOHEI; TAKIGUCHI MITSUYOSHI; ITO AKIRA; KIMURA TAKASHI; RENZAWA TAKESHI; INABA MUTSUMI, 北海道獣医師会雑誌, 47, 8, 315, 315, 01 Aug. 2003
  (公社)北海道獣医師会, Japanese
• 小動物の紐状異物の超音波画像診断
  TAKIGUCHI MITSUYOSHI; HAMAMOTO KOHEI; INABA MUTSUMI, 北海道獣医師会雑誌, 47, 8, 317, 317, 01 Aug. 2003
  (公社)北海道獣医師会, Japanese
• 悪性組織球症が疑われた犬の2例
  ITO KEI; TAKIGUCHI MITSUYOSHI; HAMAMOTO KOHEI; NAKAO YOSHIKAZU; INABA ATSUSHI, 北海道獣医師会雑誌, 47, 8, 291, 291, 01 Aug. 2003
  (公社)北海道獣医師会, Japanese
• 無菌性結節性脂肪織炎が疑われた犬の2例
  SEKIGUCHI MICHIKO; TAKIGUCHI MITSUYOSHI; INABA ATSUSHI, 北海道獣医師会雑誌, 47, 8, 302, 302, 01 Aug. 2003
  (公社)北海道獣医師会, Japanese
• Presence of B7-2(+) dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjogren's syndrome
  A Konno; K Takada; J Saegusa; M Takiguchi, AUTOIMMUNITY, 36, 4, 247, 254, Jul. 2003
  English
• Presence of B7-2(+) dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjogren's syndrome
  A Konno; K Takada; J Saegusa; M Takiguchi, AUTOIMMUNITY, 36, 4, 247, 254, Jul. 2003
  English
• 超音波検査を過小評価してはいないか?
  TAKIGUCHI MITSUYOSHI, 日本獣医循環器学会抄録, 78th, 126, 126, 21 Jun. 2003
  (一社)日本獣医循環器学会, Japanese
• 最新の画像診断装置の獣医臨床応用の可能性‐超音波検査を過小評価してはいないか?
  TAKIGUCHI MITSUYOSHI, 獣医麻酔外科学雑誌, 34, Supplement 1, 140, 140, 10 Jun. 2003
  Japanese
• Moderator band cardiomyopathyを疑った猫の一例
  濱本 耕平; 滝口 満喜; 古橋 由美子; 高橋 徹, 日本獣医循環器学会抄録, 78回, 210, 211, Jun. 2003
  (一社)日本獣医循環器学会, Japanese
• Cloning and polymorphic analysis of canine 21-hydroxyrase gene: a candidate gene for alopecia X in dogs
  TAKADA KENSUKE; KITAMURA HIROSHI; TAKIGUCHI MITSUYOSHI; SAITO MASAYUKI; HASHIMOTO AKIRA; INABA MUTSUMI, 獣医生化学, 40, 1, 21, 27, 30 Apr. 2003
  獣医生化学会, Japanese
• イヌ脱毛症Xの原因候補遺伝子：21-ヒドロキシラーゼ遺伝子のクローニングと多型解析（共著）
  獣医生化学, 40(1): 21-27, 2003
• 犬の消化管内異物の超音波画像診断
  Small Animal Clinic, 133: 22-25, 2003
• Cloning of canine 21-hydroxylase gene and its polymorphic analysis as a candidate gene for congenital adrenal hyperplasia-like syndrome in Pomeranians
  K Takada; H Kitamura; M Takiguchi; M Saito; A Hashimoto, RESEARCH IN VETERINARY SCIENCE, 73, 2, 159, 163, Oct. 2002
  English
• 急性特発性多発性神経根炎が疑われた犬の一例
  ITO HIROSHI; HAMAMOTO KOHEI; TAKIGUCHI MITSUYOSHI; OCHIAI KENJI, 北海道獣医師会雑誌, 46, 8, 292, 292, 01 Aug. 2002
  (公社)北海道獣医師会, Japanese
• The Immediate Early Gene of Canine Herpesvirus is Transcribed through Early and Late Phases
  MIYOSHI Masahiro; OKAZAKI Katsunori; TAKIGUCHI Mitsuyoshi; KIDA Hiroshi; HASHIMOTO Akira, Journal of Veterinary Medical Science, 64, 7, 627, 631, 25 Jul. 2002
  The immediate early (IE) gene of canine herpesvirus (CHV), homologue of the infected cell protein 4 (ICP4) gene of herpes simplex virus 1, is transcribed as a 4.9kb mRNA during IE phase. The IE gene was further transcribed as a 4.8kb mRNA through early (E) and late (L) phases of productive infection. Transcription of the 4.8kb mRNA initiated from downstream of the TATA box in an intron which was spliced out during IE phase. The reverse transcription-polymerase chain reaction revealed that the IE promoter was turned off during L phase at a permissive temperature. We, thus, propose to redesignate the IE gene of CHV as CICP4 gene., Japanese Society of Veterinary Science, English
• The immediate early gene of canine herpesvirus is transcribed through early and late phases
  M Miyoshi; K Okazaki; M Takiguchi; H Kida; A Hashimoto, JOURNAL OF VETERINARY MEDICAL SCIENCE, 64, 7, 627, 631, Jul. 2002
  English
• Rhabdomyosarcoma (botryoid sarcoma) of the urinary bladder in a Maltese
  M Takiguchi; T Watanabe; H Okada; T Kudo; K Yamada; J Yasuda; A Hashimoto, JOURNAL OF SMALL ANIMAL PRACTICE, 43, 6, 269, 271, Jun. 2002
  English
• 犬微小ウイルス(犬パルボウイルス1型)分離株の性状と血清疫学
  HASHIMA TAKAYUKI; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA; UNE YUMI; SASAKI NOBUO; TOOYA YUKINOBU; ONISHI TAKAFUMI; MAEDA TAKESHI; CARMICHAEL L E, 日本獣医学会学術集会講演要旨集, 133rd, 106, 106, 01 Mar. 2002
  (公社)日本獣医学会, Japanese
• 肝皮膚症候群と診断された犬の肝臓超音波画像
  Small Animal Clinic, 129: 29-31, 2002
• Association of canine minute virus(canine parvovirus type Ⅰ)with diseased puppies in Japan.
  J. Clin. Microbiol., 40, 3993, 3998, 2002
• Association of canine minute virus(canine parvovirus type Ⅰ)with diseased puppies in Japan. "jointly worked"
  J. Clin. Microbiol., 40(11);3993-3998, 2002
• 犬のすい臓疾患におけるトリプシン様免疫活性(Trypsin‐like immunoreactivity;TLI)測定の診断的意義 II 急性すい炎の病態とTLI
  TAKIGUCHI MITSUYOSHI, 動物臨床医学会年次大会プロシーディング, 22nd, 3, 181, 183, 16 Nov. 2001
  動物臨床医学会, Japanese
• A serological survey of minute virus of canines (MVC; Canine parvovirus type-1) in dogs in the Tokai area of Japan
  A Hashimoto; M Takiguchi; K Hirai; H Kida; LE Carmichael, JAPANESE JOURNAL OF VETERINARY RESEARCH, 49, 3, 249, 253, Nov. 2001
  English
• Bovine serum adenosine deaminase activity and inflammatory change of the liver.
  YASUDA JUN; TAKUMA SHUNSUKE; TAKIGUCHI MITSUYOSHI; OKADA KEIJI; HASHIMOTO AKIRA, 獣医生化学, 38, 2, 33, 37, 31 Oct. 2001
  獣医生化学会, Japanese
• ウマヘルペスウイルス9型(EHV‐9)経鼻接種によるシリアンハムスター脳炎モデルのMRIによる経時的観察
  SUZUKI KENGO; ASANUMA TAKETOSHI; FUKUSHI HIDETO; HIRAI KATSUYA; TAKIGUCHI MITSUYOSHI; KUWAHARA MIKINORI; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 132nd, 221, 221, 07 Sep. 2001
  (公社)日本獣医学会, Japanese
• 非定形型副腎皮質機能低下症の犬の一例
  ITO KEI; TAKIGUCHI MITSUYOSHI; TAKADA KENSUKE; HAMAMOTO KOHEI; HASHIMOTO AKIRA, 北海道獣医師会雑誌, 45, 8, 272, 272, 01 Aug. 2001
  (公社)北海道獣医師会, Japanese
• 副腎皮質機能低下の猫の一例
  WATANABE MINAKO; TAKIGUCHI MITSUYOSHI; TAKADA KENSUKE; HAMAMOTO KOHEI; HASHIMOTO AKIRA, 北海道獣医師会雑誌, 45, 8, 272, 272, 01 Aug. 2001
  (公社)北海道獣医師会, Japanese
• Malignant seminoma with systemic metastases in a dog
  M Takiguchi; T Iida; T Kudo; A Hashimoto, JOURNAL OF SMALL ANIMAL PRACTICE, 42, 7, 360, 362, Jul. 2001
  English
• ぼうこう・尿道疾患のX線・超音波診断
  TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA, 獣医麻酔外科学雑誌, 32, Supplement 1, 67, 68, 01 Jul. 2001
  (一社)日本獣医麻酔外科学会, Japanese
• 犬の副腎褐色細胞腫の超音波診断
  TAKIGUCHI MITSUYOSHI; HAMAMOTO KOHEI; TAKADA KENSUKE; HASHIMOTO AKIRA, 獣医麻酔外科学雑誌, 32, Supplement 1, 291, 292, 01 Jul. 2001
  (一社)日本獣医麻酔外科学会, Japanese
• 自己免疫疾患の遺伝子治療
  TAKIGUCHI MITSUYOSHI, 日本獣医学会学術集会講演要旨集, 131st, 37, 37, 05 Mar. 2001
  (公社)日本獣医学会, Japanese
• すい管結さつマウスモデルを用いたすい臓におけるc‐metの発現動態
  HAMAMOTO KOHEI; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 131st, 142, 142, 05 Mar. 2001
  (公社)日本獣医学会, Japanese
• 犬微小ウイルス(犬パルボウイルス1型)の遺伝子の検出とウイルス分離
  MOCHIZUKI MASAMI; HASHIMOTO MICHIRU; HASHIMOTO AKIRA; TAKIGUCHI MITSUYOSHI; CARMICHAEL L E; PARRISH C R, 日本獣医学会学術集会講演要旨集, 131st, 81, 81, 05 Mar. 2001
  (公社)日本獣医学会, Japanese
• 膵管結紮マウスモデルを用いた膵臓におけるc-metの発現動態
  濱本 耕平; 滝口 満喜; 橋本 晃, 日本獣医学会学術集会講演要旨集, 131回, 142, 142, Mar. 2001
  (公社)日本獣医学会, Japanese
• An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies
  T Watanabe; N Shinohara; A Sazawa; T Harabayashi; Y Ogiso; T Koyanagi; M Takiguchi; A Hashimoto; N Kuzumaki; M Yamashita; M Tanaka; HB Grossman; WF Benedict, CANCER GENE THERAPY, 7, 12, 1575, 1580, Dec. 2000
  English
• Canine extracutaneous mast-cell tumours consisting of connective tissue mast cells
  N Iwata; K Ochiai; T Kadosawa; M Takiguchi; T Umemura, JOURNAL OF COMPARATIVE PATHOLOGY, 123, 4, 306, 310, Nov. 2000
  English
• イヌの成長ホルモン反応性脱毛症における21‐ヒドロキシラーゼ遺伝子の検討
  TAKADA KENSUKE; KITAMURA HIROSHI; TAKIGUCHI MITSUYOSHI; SAITO MASAYUKI; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 130th, 123, 07 Sep. 2000
  Japanese
• 新しいぼうこう癌同所性移植モデルを用いた経尿道的アデノウイルスベクター注入による遺伝子治療の有用性の検討
  WATANABE TAKAFUMI; SHINOHARA NOBUO; SAZAWA YO; OGISO YOSHIFUMI; TAKIGUCHI MITSUYOSHI; KUZUMAKI SUSUMU; YAMASHITA MOTOYUKI; TANAKA MOTOMIKI; GROSSMAN H B, 日本獣医学会学術集会講演要旨集, 130th, 27, 07 Sep. 2000
  Japanese
• 蛋白喪失性腸症に対する酢酸オクトレオチドによる新しい治療法の試み
  TAKIGUCHI MITSUYOSHI; HAMAMOTO KOHEI; HASHIMOTO AKIRA, 北海道獣医師会雑誌, 44, 7, 257, 257, 01 Jul. 2000
  (公社)北海道獣医師会, Japanese
• 2‐クロロデオキシアデノシン(2‐CdA)を用いたネコ縦隔型リンパ腫に対する新しい治療法の可能性
  FUJIKI MEGUMI; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA, 北海道獣医師会雑誌, 44, 7, 244, 244, 01 Jul. 2000
  (公社)北海道獣医師会, Japanese
• CTLA4IgG gene delivery prevents autoantibody production and lupus nephritis in MRL/lpr mice
  M Takiguchi; M Murakami; Nakagawa, I; A Hashimoto; T Uede, FASEB JOURNAL, 14, 6, A1207, A1207, Apr. 2000
  English, Summary international conference
• Significance of the Grb2 and Son of sevenless (Sos) proteins in human bladder cancer cell lines
  T Watanabe; N Shinohara; K Moriya; A Sazawa; Y Kobayashi; Y Ogiso; M Takiguchi; J Yasuda; T Koyanagi; N Kuzumaki; A Hashimoto, IUBMB LIFE, 49, 4, 317, 320, Apr. 2000
  English
• Significance of the Grb2 and Son of sevenless (Sos) proteins in human bladder cancer cell lines
  T Watanabe; N Shinohara; K Moriya; A Sazawa; Y Kobayashi; Y Ogiso; M Takiguchi; J Yasuda; T Koyanagi; N Kuzumaki; A Hashimoto, IUBMB LIFE, 49, 4, 317, 320, Apr. 2000
  English
• ぼうこう内腫りゅう性病変の画像所見を示したぼうこう血腫の1例
  WATANABE TAKAFUMI; TAKIGUCHI MITSUYOSHI; KUDO TADAAKI; HASHIMOTO AKIRA, 北海道獣医師会雑誌, 44, 4, 104, 106, 01 Apr. 2000
  Japanese
• イヌヘルペスウイルス(CHV)感染新生子犬の脳病変におけるウイルス抗原及びウイルスゲノムの分布
  NISHINO AKI; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA; OKAZAKI KATSUNORI; KIDA HIROSHI, 日本獣医学会学術集会講演要旨集, 129回, 47, 47, Mar. 2000
  (公社)日本獣医学会, Japanese
• CTLA4IgG gene delivery prevents autoantibody production and lupus nephritis in MRL/lpr mice
  M Takiguchi; M Murakami; Nakagawa, I; Saito, I; A Hashimoto; T Uede, LIFE SCIENCES, 66, 11, 991, 1001, Feb. 2000
  English
• CTLA4IgG gene delivery prevents autoantibody production and lupus nephritis in MRL/lpr mice
  M Takiguchi; M Murakami; Nakagawa, I; Saito, I; A Hashimoto; T Uede, LIFE SCIENCES, 66, 11, 991, 1001, Feb. 2000
  English
• Suppressive effects of dominant negative ras mutant N116Y on transformed phenotypes of human bladder cancer cells
  T Watanabe; N Shinohara; A Sazawa; Y Kobayashi; Y Ogiso; T Kimura; M Takiguchi; J Yasuda; A Hashimoto; T Koyanagi; N Kuzumaki, CANCER LETTERS, 149, 1-2, 195, 202, Feb. 2000
  English
• 膀胱内腫瘤性病変の画像所見を示した膀胱血腫の1例(共著)
  渡邉 孝文; 滝口 満喜; 工藤 忠明; 橋本 晃, 北海道獣医師会雑誌, 44, 4, 104, 106, 2000
  (公社)北海道獣医師会, Japanese
• Involvement of CD28/CTLA4-B7 costimulatory pathway in the development of lymphadenopathy and splenomegaly in MRL/lpr mice
  M Takiguchi; M Murakami; Nakagawa, I; MM Rashid; N Tosa; S Chikuma; A Hashimoto; T Uede, JOURNAL OF VETERINARY MEDICAL SCIENCE, 62, 1, 29, 36, Jan. 2000
  English
• Structure of the infected cell protein 0 gene of canine herpesvirus
  M Miyoshi; M Takiguchi; J Yasuda; A Hashimoto; A Takada; K Okazaki; H Kida, ARCHIVES OF VIROLOGY, 145, 8, 1715, 1723, 2000
  English
• Involvement of CD28/CTLA4-B7 costimulatory pathway in the development of lymphadenopathy and splenomegaly in MRL/lpr mice
  M Takiguchi; M Murakami; Nakagawa, I; MM Rashid; N Tosa; S Chikuma; A Hashimoto; T Uede, JOURNAL OF VETERINARY MEDICAL SCIENCE, 62, 1, 29, 36, Jan. 2000
  English
• Structure of the infected cell protein O gene of canine herpesvirus. "jointly worked"
  Arch. Virol., 145, 8, 1715, 1723, 2000
• Development of an Ultrasonic Washing Machine for Animals and its Clinical Application to Chronic Skin Diseases of Dogs.
  YASUDA JUN; NAKAO KIICHI; SHIMIZU KYOKO; KAMATA SATORU; KITAMURA YUKITOSHI; TAKIGUCHI MITSUYOSHI, 獣医畜産新報, 52, 939, 801, 804, 01 Oct. 1999
  文永堂出版, Japanese
• Dominant negative ras mutant N116Y発現アデノウイルスベクターを用いたぼうこう癌に対する遺伝子治療の基礎的研究
  WATANABE TAKAFUMI; SHINOHARA NOBUO; SAZAWA AKIRA; TAKIGUCHI MITSUYOSHI; KOYANAGI TOMOHIKO; KATSUMAKI SUSUMU; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 128th, 186, 186, 01 Sep. 1999
  (公社)日本獣医学会, Japanese
• 脳包虫症ラットモデルのMRI画像観察
  MATSUMOTO YUKARI; ASANUMA TAKETOSHI; NAKAO MINORU; NAKATANI KAZUHIRO; TAKIGUCHI MITSUYOSHI; ITO RYO; KUWABARA MIKINORI; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 128th, 167, 167, 01 Sep. 1999
  (公社)日本獣医学会, Japanese
• CTLA4IgG発現アデノウイルスベクターを用いたループス腎炎への遺伝子治療の試み
  TAKIGUCHI MITSUYOSHI; MURAKAMI MASAAKI; NAKAGAWA IZUMI; SAITO IZUMI; HASHIMOTO AKIRA; KAMIDE TOSHIMITSU, 日本獣医学会学術集会講演要旨集, 128th, 20, 20, 01 Sep. 1999
  (公社)日本獣医学会, Japanese
• 犬副腎髄質褐色細胞腫の画像診断と尿中カテコールアミン代謝産物の診断的意義
  滝口 満喜; 宮田 佳代子; 橋本 晃, 北海道獣医師会雑誌, 43, 8, 256, 256, Aug. 1999
  (公社)北海道獣医師会, Japanese
• インスリン療法により重度の溶血性貧血を呈した糖尿病性ケトアシドーシスの猫の1例
  濱本 耕平; 滝口 満喜; 橋本 晃, 北海道獣医師会雑誌, 43, 8, 253, 253, Aug. 1999
  (公社)北海道獣医師会, Japanese
• Combination of CTLA41GG and FTY720 suppresses development of obliterative bronchiolitis and respiratory epithelial injury in a murine heterotopic airway model
  K Konishi; M Murakami; A Yamada; M Takiguchi; M Morikawa; T Omura; S Todo; T Uede, TRANSPLANTATION, 67, 9, S605, S605, May 1999
  English, Summary international conference
• Detection of canine herpesvirus DNA in the ganglionic neurons and the lymph node lymphocytes of latently infected dogs
  M Miyoshi; Y Ishii; M Takiguchi; A Takada; J Yasuda; A Hashimoto; K Okazaki; H Kida, JOURNAL OF VETERINARY MEDICAL SCIENCE, 61, 4, 375, 379, Apr. 1999
  English
• Blockade of CD28/CTLA4-B7 pathway prevented autoantibody-related diseases but not lung disease in MRL/lpr mice
  M Takiguchi; M Murakami; Nakagawa, I; A Yamada; S Chikuma; Y Kawaguchi; A Hashimoto; T Uede, LABORATORY INVESTIGATION, 79, 3, 317, 326, Mar. 1999
  English
• MRL/lprマウス肺病変形成におけるCD28/CTLA4‐B7補助シグナルの役割
  TAKIGUCHI MITSUYOSHI; MURAKAMI MASAAKI; NAKAGAWA IZUMI; YASUDA JUN; HASHIMOTO AKIRA; KAMIDE TOSHIMITSU, 日本獣医学会学術集会講演要旨集, 127th, 68, 68, 01 Mar. 1999
  (公社)日本獣医学会, Japanese
• 動物用超音波洗浄装置を用いた犬の慢性皮膚疾患の治療について
  NAKAO KIICHI; YASUDA JUN; SHIMIZU KYOKO; KITAMURA YUKITOSHI; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA, 日本産業動物獣医学会・日本小動物獣医学会・日本獣医公衆衛生学会年次大会, 1998, 362, Feb. 1999
  Japanese
• X線および超音波検査で副腎髄質腫ようが疑われた犬の一例
  MIYATA KAYOKO; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA, 日本産業動物獣医学会・日本小動物獣医学会・日本獣医公衆衛生学会年次大会, 1998, 354, Feb. 1999
  Japanese
• 犬の皮膚病に対する動物用超音波洗浄装置の応用(共著)
  安田 準; 中尾 喜一; 清水 恭子; 釜田 悟; 北村 之利; 滝口 満喜, 獣医畜産新報, 52, 10, 801, 804, 1999
  文永堂出版(株), Japanese
• Structure of the immediate early gene of canine herpesvirus - Brief report
  M Miyoshi; M Takiguchi; J Yasuda; A Hashimoto; A Takada; K Okazaki; H Kida, ARCHIVES OF VIROLOGY, 144, 2, 407, 420, 1999
  English
• Preliminary study on clinical application of endoscopic ultrasonography (EUS) in small animal practice: EUS findings of experimentally-induced cystic lesion in the gastric wall in dogs (in Japanese with English Summary) 'jointly worked'
  Jpn. J. Vet. Imag, 10, 37, 40, 1999
• Structure of immediate early gene of canine herpesvirus. "jointly worked"
  Arch. Virol., 144, 2, 407, 420, 1999
• Detection of canine herpesvirns DNA in the ganglionic neurons and the lymph node lymphocytes of latently infected dogs. "jointly worked"
  Masahiro Miyoshi; Yuki Ishii; Mitsuyoshi Takiguchi; Ayato Takada; Jun Yasuda; Akira Hashimoto; Katsunori Okazaki; Hiroshi Kida, J. Ved. Med. Sci., 61, 4, 375, 379, 1999
• Preliminary study on clinical application of endoscopic ultrasonography (EUS) in small animal practice: EUS findings of experimentally-induced cystic lesion in the gastric wall in dogs (in Japanese with English Summary) 'jointly worked'
  Jpn. J. Vet. Imag, 10, 37, 40, 1999
• Laboratory of Pathobiology
  HASHIMOTO Akira; TAKIGUCHI Mitsuyoshi, Japanese journal of veterinary research, 46, 2, 90, 91, 30 Nov. 1998
  Hokkaido University, English
• Endoscopic ultrasonography of the pancreas in the dog
  Y Morita; M Takiguchi; J Yasuda; T Kitamura; M Syakalima; KD Eom; A Hashimoto, VETERINARY RADIOLOGY & ULTRASOUND, 39, 6, 552, 556, Nov. 1998
  English
• Endoscopic ultrasonographic findings of the pancreas after pancreatic duct ligation in the dog
  Y Morita; M Takiguchi; J Yasuda; KD Eom; A Hashimoto, VETERINARY RADIOLOGY & ULTRASOUND, 39, 6, 557, 562, Nov. 1998
  English
• 1prマウス肺病変形成におけるCD28/CTLA4-B7補助シグナルの役割
  滝口 満喜; 村上 正晃; 中川 泉; 橋本 晃; 上出 利光, 日本臨床免疫学会会誌, 26回抄録集, 376, 376, Oct. 1998
  (一社)日本臨床免疫学会, Japanese
• lprマウス肺病変形成におけるCD28/CTLA4‐B7補助シグナルの役割
  TAKIGUCHI MITSUYOSHI; MURAKAMI MASAAKI; NAKAGAWA IZUMI; HASHIMOTO AKIRA; UEDE TOSHIMITSU, 日本免疫学会総会・学術集会記録, 28, 376, Oct. 1998
  Japanese
• Isoenzyme Diagnosis in Veterinary Small Animal Medicine.
  YASUDA JUN; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA; SYAKALIMA M S, 動物臨床医学, 7, 2, 57, 65, Sep. 1998
  Japanese
• 犬の血中アデノシンデアミナーゼ活性の臨床的意義について
  安田 準; 高橋 恵理子; 滝口 満喜; 橋本 晃, 北海道獣医師会雑誌, 42, 8, 290, 290, Aug. 1998
  (公社)北海道獣医師会, Japanese
• Pathohistological finding supplement of pancreas acinus atrophy of dog.
  AZUMA YOKO; TAKIGUCHI MITSUYOSHI; YASUDA JUN; OCHIAI KENJI; KUDO TADAAKI; IWANAGA TOSHIHIKO; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 126th, 263, 263, Aug. 1998
  (公社)日本獣医学会, Japanese
• Concern of CD28/CTLA4-B7 support signal in pathogeny of MRL/lpr mouse.
  TAKIGUCHI MITSUYOSHI; MURAKAMI MASAAKI; NAKAGAWA IZUMI; YASUDA JUN; HASHIMOTO AKIRA; KAMIIDE TOSHIMITSU, 日本獣医学会学術集会講演要旨集, 126th, 22, 22, Aug. 1998
  (公社)日本獣医学会, Japanese
• Endoscopic and transcutaneous ultrasonographic findings and grey-scale histogram analysis in dogs with caerulein-induced pancreatitis
  Y Morita; M Takiguchi; J Yasuda; T Kitamura; M Syakalima; A Hashimoto, VETERINARY QUARTERLY, 20, 3, 89, 92, Jul. 1998
  English
• Preliminary studies on clinical application of endoscopic ultrasonography(EUS) in small animal practice: EUS findings of experimentally-induced cystic lesion in the gastric wall in dogs.
  MORITA YUTAKA; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA, 獣医画像診断, 10, 37, 40, Jun. 1998
  Japanese
• The canine alkaline phosphatases: A review of the isoenzymes in serum, analytical methods and their diagnostic application
  M Syakalima; M Takiguchi; J Yasuda; A Hashimoto, JAPANESE JOURNAL OF VETERINARY RESEARCH, 46, 1, 3, 11, May 1998
  English, Book review
• Blockade of CD28/CTLA4 pathway prevented almost diseases and lymphadenopathy but not lung disease in MRL/lpr mice.
  M Takiguchi; M Murakami; Nakagawa, I; A Yamada; S Chikuma; T Uede, FASEB JOURNAL, 12, 5, A1093, A1093, Mar. 1998
  English, Summary international conference
• Ultrasonographic and serologic studies of experimental cysticercosis in rats infected with Taenia taeniaeformis
  A Ito; Y Sakakibara; L Ma; K Asano; M Takiguchi; J Yasuda; A Hashimoto, PARASITE IMMUNOLOGY, 20, 3, 105, 110, Mar. 1998
  English
• Ultrasonographic and serologic studies of experimental cysticercosis in rats infected with Taenia taeniaeformis
  A Ito; Y Sakakibara; L Ma; K Asano; M Takiguchi; J Yasuda; A Hashimoto, PARASITE IMMUNOLOGY, 20, 3, 105, 110, Mar. 1998
  English
• Identification of the latent infected part of canine herpesvirus.
  MIYOSHI MASAHIRO; ISHII YUKI; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA; TAKADA AYAHITO; OKAZAKI KATSUNORI; KIDA HIROSHI, 日本獣医学会学術集会講演要旨集, 125回, 140, 140, Mar. 1998
  (公社)日本獣医学会, Japanese
• Trial of quantitative analysis of alkaline phosphatase isoenzyme in canine.
  SYAKALIMA M; YASUDA JUN; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 125th, 271, 271, Mar. 1998
  (公社)日本獣医学会, Japanese
• Application of serum trypsin-like immunoactivity (TLI) measurement to dog pancreas external hyposecretion.
  森田豊; 滝口満喜; 安田準; 橋本晃, 日本産業動物獣医学会・日本小動物獣医学会・日本獣医公衆衛生学会年次大会, 1997, 292, 293, Feb. 1998
  Japanese
• 超音波内視鏡検査(EUS)の小動物臨床への応用に関する研究－イヌの実験的胃壁嚢胞性病変のEUS画像(予報)（共著）
  獣医画像診断, 10: 37-40, 1998
• 小動物臨床におけるアイソザイムの診断（共著）
  動物臨床医学, 7: 57-65, 1998
• Comparison of attenuation and liver-kidney contrast of liver ultrasonographs with histology and biochemistry in dogs with experimentally induced steroid hepatopathy
  M Syakalima; M Takiguchi; J Yasuda; Y Morita; A Hashimoto, VETERINARY QUARTERLY, 20, 1, 18, 22, Jan. 1998
  English
• Separation and quantification of corticosteroid-induced, bone and liver alkaline phosphatase isoenzymes in canine serum
  M Syakalima; M Takiguchi; J Yasuda; A Hashimoto, JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 44, 9-10, 603, 610, Dec. 1997
  English
• イヌにおける年齢別の血清中ALP分画活性値の定量及びステロイド誘発性ALP分画活性値の診断的意義
  Syakalima Michelo; 滝口 満喜; 安田 準, The Journal of Veterinary Medical Science, 59, 10, 905, 909, Oct. 1997
  (公社)日本獣医学会, English
• The age dependent levels of serum ALP isoenzymes and the diagnostic significance of corticosteroid-induced ALP during long-term glucocorticoid treatment
  M Syakalima; M Takiguchi; J Yasuda; A Hashimoto, JOURNAL OF VETERINARY MEDICAL SCIENCE, 59, 10, 905, 909, Oct. 1997
  English
• Ultrasonographic appearance of orthotopic ureterocele in a dog
  M Takiguchi; J Yasuda; K Ochiai; Y Morita; A Hashimoto, VETERINARY RADIOLOGY & ULTRASOUND, 38, 5, 398, 399, Sep. 1997
  English
• Structure of the pre-early gene of canine herpesvirus.
  MIYOSHI MASAHIRO; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA; OKAZAKI KATSUNORI; KIDA HIROSHI, 日本獣医学会学術集会講演要旨集, 124th, 150, Sep. 1997
  Japanese
• MRI image observation of a lung echinococcosis rat model.
  KAWAHARA TOKIKO; ASANUMA TAKETOSHI; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA; KUWAHARA MIKINORI; MA RYO; ITO AKIRA, 日本獣医学会学術集会講演要旨集, 124th, 43, Sep. 1997
  Japanese
• Insulin stimulated secretion test by the 10% arginine hydrochloride load in the cat.
  KITAMURA TOMISHIGEKO; MORITA YUTAKA; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 124th, 199, Sep. 1997
  Japanese
• On the clinical significance of bovine serum adenosine deaminase activity.
  YASUDA JUN; TAKEMA SHUNSUKE; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 124th, 200, Sep. 1997
  Japanese
• Research on the application of endoscopic ultrasonic inspection (EUS) to small- animal clinic. (II). Hystogram analysis of the pancreas substance echo luminance of a dog having received pancreatic duct ligation.
  MORITA YUTAKA; KITAMURA TOSHIKO; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 124th, 42, Sep. 1997
  Japanese
• Intrahepatic lobular distribution of alkaline phosphatase (ALP) isoenzymes in the dog glucocorticoid induced hepatic disorder.
  MICHERO SHAKARIMA; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 124th, 200, Sep. 1997
  Japanese
• 超音波検査が有用であった犬の腸閉塞の3例
  滝口 満喜, 北海道獣医師会雑誌, 41, 8, 276, 276, Aug. 1997
  (公社)北海道獣医師会, Japanese
• Short-term hemodialysis treatment in dogs and cats with total uretic obstruction
  T Mashita; J Yasuda; M Iijima; M Takiguchi; T Yamazaki; A Hashimoto, JAPANESE JOURNAL OF VETERINARY RESEARCH, 45, 2, 59, 65, Aug. 1997
  English
• Circumstances on small animal medical care in U.S.A.
  TAKIGUCHI MITSUYOSHI, 北海道獣医師会雑誌, 41, 4, 91, 94, Apr. 1997
  (公社)北海道獣医師会, Japanese
• Study of application of ultrasonic autoscope inspection (EUS) to small animal clinic. Histogram analysis of pancreas substance echo luminance with caerulein induced pancreatitis of dog.
  MORITA YUTAKA; KITAMURA TOSHIKO; TAKIGUCHI MITSUYOSHI; YASUDA JUN; HASHIMOTO AKIRA, 日本獣医学会学術集会講演要旨集, 123rd, 255, Mar. 1997
  Japanese
• Esophageal/gastric adenocarcinoma in a dog
  M Takiguchi; J Yasuda; A Hashimoto; K Ochiai; C Itakura, JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION, 33, 1, 42, 44, Jan. 1997
  English
• Ultrasonographic renal changes associated with phenylbutazone administration in three foals
  R Leveille; T Miyabayashi; SE Weisbrode; DS Biller; M Takiguchi; JF Williams, CANADIAN VETERINARY JOURNAL-REVUE VETERINAIRE CANADIENNE, 37, 4, 235, 236, Apr. 1996
  English
• Ultrasonic image of liver in cat cestode infected rat.
  SAKAKIBARA YOSHIMI; TAKIGUCHI MITSUYOSHI; HASHIMOTO AKIRA; YASUDA JUN; ITO AKIRA, 日本獣医学会学術集会講演要旨集, 121st, 109, Mar. 1996
  Japanese
• Ultrasonographic Examination of Cephaloridine (Cer)-Induced Renal Lesions in Rabbits
  Katsuhiko Ishida; Akira Hashimoto; Jun Yasuda; Mitsuyoshi Takiguchi; Kunio Doi, Journal of Toxicologic Pathology, 9, 3, 273, 276, 1996
  English
• Ultrasonographic Examination of Cephaloridine (Cer)-Induced Renal Lesions in Rabbits
  Katsuhiko Ishida; Akira Hashimoto; Jun Yasuda; Mitsuyoshi Takiguchi; Kunio Doi, Journal of Toxicologic Pathology, 9, 3, 273, 276, 1996
  English
• RADIOGRAPHIC ANATOMY OF THE MEDIAL CORONOID PROCESS OF DOGS
  T MIYABAYASHI; M TAKIGUCHI; SC SCHRADER; DS BILLER, JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION, 31, 2, 125, 132, Mar. 1995
  English
• 抗血清およびアシクロビルによる新生仔犬の犬ヘルペスウイルス感染症の発病阻止効果
  浅野真知; 滝口満喜; 安田準; 橋本晃; 喜田宏; 平井克哉, 日本獣医学会学術集会講演要旨集, 119th, 235, Mar. 1995
  Japanese
• 新しい臨床診断 キモパパインを用いた髄核融解後のMRI像に関する試験的研究
  滝口満喜; 宮林孝仁, 日本獣医学会学術集会講演要旨集, 118th, 65, Aug. 1994
  Japanese
• 臨床生化学 小動物臨床における尿中酵素の診断的意義
  松岡鎮雄; 真下忠久; 森田豊; 滝口満喜; 安田準; 山崎忠良; SYAKALIMA M; 橋本晃, 日本獣医学会学術集会講演要旨集, 118th, 25, Aug. 1994
  Japanese
• RADIOGRAPHIC APPEARANCES OF THE NASAL CONCHAE IN DOGS USING DIFFERENT SCREEN-FILM SYSTEMS - A POSTMORTEM STUDY
  T MIYABAYASHI; DS BILLER; PR HAIDER; M TAKIGUCHI, JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION, 30, 4, 382, 388, Jul. 1994
  English
• Degeneration and diseases of intervertebral disk of the dog.
  TAKIGUCHI MITSUYOSHI; MIYABAYASHI TAKAHITO, 小動物臨床, 13, 2, 13, 18, Mar. 1994
  Japanese
• SCINTIGRAPHIC AND ULTRASONOGRAPHIC DIAGNOSIS OF SOFT-TISSUE INJURY IN A THOROUGHBRED HORSE
  J WILLIAMS; T MIYABAYASHI; A RUGGLES; J YAMAMOTO; M TAKIGUCHI, JOURNAL OF VETERINARY MEDICAL SCIENCE, 56, 1, 169, 172, Feb. 1994
  English, Introduction scientific journal
• 犬の椎間板の変性と疾患(共著)
  滝口 満喜; 宮林 孝仁, 小動物臨床, 13, 2, 13, 18, 1994
  (株)山水書房, Japanese
• Scintigraphic and ultrasonographic diagnosis of soft tissue injury in a thoroughberd horse. "jointly worked"
  J. Vet. Med. Sci., 53, 6, 1019, 1024, 1994
• アメリカにおける獣医免許制度について
  宮林孝仁; 滝口満喜, 小動物臨床, 12, 5, 31, 33, Sep. 1993
  (株)山水書房, Japanese
• 犬猫の口腔内腫瘍(共著)
  滝口 満喜; 宮林 孝仁, 小動物臨床, 12, 1, 11, 14, 1993
  (株)山水書房, Japanese
• Tumor in oral cavity of dog and cat.
  TAKIGUCHI MITSUYOSHI; MIYABAYASHI TAKAHITO, 小動物臨床, 12, 1, 11, 14, Jan. 1993
  Japanese
• Acute phase reaction protein of a horse.
  OKUMURA MASAHIRO; YAMASHITA KAZUHITO; TAIRA TOMOKO; ASANO KAZUYUKI; FUKAWA YASUSHI; TAKIGUCHI MITSUYOSHI; FUJINAGA TOORU, 家畜生化学研究会報, 29, 9, 18, Sep. 1992
  Japanese
• ウマの急性相蛋白（共著）
  家畜生化学研究会報, 29: 9-18, 1992
• SERUM C-REACTIVE PROTEIN (CRP) IN HORSES - THE EFFECT OF AGING, SEX, DELIVERY AND INFLAMMATIONS ON ITS CONCENTRATION
  K YAMASHITA; T FUJINAGA; M OKUMURA; M TAKIGUCHI; N TSUNODA; S MIZUNO, JOURNAL OF VETERINARY MEDICAL SCIENCE, 53, 6, 1019, 1024, Dec. 1991
  English
• SERUM C-REACTIVE PROTEIN (CRP) IN HORSES - THE EFFECT OF AGING, SEX, DELIVERY AND INFLAMMATIONS ON ITS CONCENTRATION
  K YAMASHITA; T FUJINAGA; M OKUMURA; M TAKIGUCHI; N TSUNODA; S MIZUNO, JOURNAL OF VETERINARY MEDICAL SCIENCE, 53, 6, 1019, 1024, Dec. 1991
  English
• 猫の外傷性口蓋破裂の外科的閉鎖法(共著)
  日本動物病院協会雑誌, 22: 12-16, 1991
• ISOLATION, CHARACTERIZATION, AND QUANTITATIVE-ANALYSIS OF C-REACTIVE PROTEIN FROM HORSES
  M TAKIGUCHI; T FUJINAGA; M NAIKI; S MIZUNO; K OTOMO, AMERICAN JOURNAL OF VETERINARY RESEARCH, 51, 8, 1215, 1220, Aug. 1990
  English
• Massive subcutaneous hemangiosarcoma in a dog.
  FUJINAGA TORU; SUWA TAKAHIKO; NARIKIO MICHIYO; OKUMURA MASAHIRO; INOUE KUMIKO; TAKIGUCHI MITSUYOSHI; MURAKAMI MASAAKI; TAJIMA MOTOSHI; OTOMO KANJURO, 日本獣医師会雑誌, 42, 7, 491, 494, Jul. 1989
  Japanese
• ウマの急性相蛋白に関する研究(1) ウマC-反応性蛋白の分離・精製およびその定量
  滝口 満喜, 日本獣医学会学術集会講演要旨集, 107回, 227, 227, Mar. 1989
  (公社)日本獣医学会, Japanese
• Massive subcutaneous hemangiosarcoma in a dog.
  藤永徹; 諏訪隆彦; 成清美智代; 奥村正裕; 井上久美子; 滝口満喜; 村上正晃; 田島誉士; 大友勘十郎, 日本獣医師会雑誌, 42: 491-494, 7, 1989
• Masive subcutaneous hemangiosarcoma in a dog (in Jaoanese with English summary) 'jointly worked'
  J. Jpn. Vet. Assoc., 3, 491, 494, 1989
• Isolation, characterization, and quantitative analysis of C-reactive protein from horses. "jointly worked"
  TAKIGUCHI, Mitsuyoshi, Jap. J. Vet. Res., 37, 2, 134, 134, 1989
  English
• Masive subcutaneous hemangiosarcoma in a dog (in Jaoanese with English summary) 'jointly worked'
  J. Jpn. Vet. Assoc., 3, 491, 494, 1989