Researcher basic information

• 博士（獣医学）, 北海道大学大学院獣医学研究科, Mar. 2012

• https://researchmap.jp/sunaga21?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202001009620263048

• 90649112

• 202001009620263048

• 獣医整形外科

• Life Science, Veterinary medical science, 整形外科

• Bachelor's degree program, School of Veterinary Medicine
• Doctoral (PhD) degree program, Graduate School of Veterinary Medicine

Research activity information

• Effect of fuzapladib sodium hydrate on adhesion molecule expression in canine endothelial cells and neutrophils.
  Kanittha Darawiroj; Takafumi Sunaga; Ryo Owaki; Nomsa Mulenga Handondo; Masahiro Okumura
  The Journal of veterinary medical science, 31 Dec. 2025, [Domestic magazines]
  English, Scientific journal, Neutrophil recruitment to inflamed tissue is mediated by adhesion molecules and chemokine signaling. Fuzapladib sodium hydrate (FZP) has been reported to reduce neutrophil infiltration, but its broader effect on adhesion molecules in dogs remains unclear. This study investigated the influence of FZP on canine aortic endothelial cells (CnAOEC) and primary neutrophils under cytokine stimulation. CnAOEC were treated with recombinant canine tumor necrosis factor alpha (rh-cTNFα) or lipopolysaccharide together with FZP, while neutrophils isolated from healthy dogs were preincubated with FZP before rh-cTNFα stimulation for gene and protein expression analyses. Expression of endothelial E-selectin, P-selectin, and intercellular adhesion molecule-1 (ICAM-1) was quantified by qPCR. Neutrophil C-X-C motif chemokine receptor 2, P-selectin glycoprotein ligand-1 (PSGL-1), and L-selectin were also analyzed using qPCR. In addition, C-X-C motif chemokine ligand 1 and leukocyte function-associated antigen-1 (LFA-1) were determined using ELISA and flow cytometry, respectively. Neutrophil adhesion to FZP-treated endothelial monolayers was evaluated under static conditions. FZP significantly downregulated endothelial P-selectin at the highest dose, whereas ICAM-1 was upregulated. In neutrophils, FZP decreased L-selectin and PSGL-1 expression at the highest concentration. These findings suggest that FZP selectively modulates early adhesion through suppression of P-selectin, L-selectin, and PSGL-1, which may weaken rolling and tethering interactions between cells. Although functional adhesion effects were limited, these molecular responses provide further insight into the anti-inflammatory effect of FZP.
• Progressive myelomalacia with spinal cord disorders along with severe intradural hemorrhage in a cat.
  Takafumi Sunaga; Shingo Miki; Fuka Takahashi; Keisuke Aoshima; Sangho Kim; Masahiro Okumura
  The Journal of veterinary medical science, 87, 8, 954, 959, 01 Aug. 2025, [Domestic magazines]
  English, Scientific journal, Progressive myelomalacia (PMM) is a severe neurological disorder. Although several case reports have been published, PMM is uncommon in cats. A 9-year-old neutered male domestic short-haired cat presented with hindlimb paraplegia. Based on the neurological examination, severe abnormalities in the L4-S3 segment were suspected. Based on magnetic resonance imaging findings, intervertebral disk herniation (IVDH) was suspected at the L5-L6 intervertebral disk. A hemilaminectomy and durotomy were performed. Four days after surgery, PMM was suspected, and the cat was euthanized. An autopsy was performed, and a histopathological examination confirmed PMM secondary to severe spinal disorders.
• Glutathione-associated redox regulation alleviates radio-resistance of canine cancer stem-like cells with low proteasome activity.
  Koangyong Sung; Kenji Hosoya; Tatsuya Deguchi; Koya Yamashita; Sangho Kim; Takafumi Sunaga; Hironobu Yasui; Osamu Inanami; Masahiro Okumura
  Scientific reports, 15, 1, 18017, 18017, 23 May 2025, [International Magazine]
  English, Scientific journal, Radio-resistance of cancer stem-like cells (CSCs) is associated with the failure of radiation therapy. ZsGreen1-positive (ZsG⁺) cells, which exhibit low proteasome activity, have been used to enable the detection and isolation of CSCs. However, the mechanisms of radio-resistance in canine tumor cells with low proteasome activity remain unclear. This study aimed to elucidate the radio-resistance mechanisms of ZsG+ cells by identifying a potential target of canine CSCs. ZsG+ cells, isolated using flow cytometric cell sorting, were compared with ZsG- cells. Sulfasalazine was used to suppress glutathione (GSH) synthesis by inhibiting xCT. In vitro experiments demonstrated a significantly higher radio-resistance in ZsG+ cells than in ZsG- cells. After X-irradiation, ZsG+ cells had fewer p53‑binding protein 1 (53BP1) foci, low reactive oxygen species (ROS) accumulation, and high GSH content. Sulfasalazine caused radiosensitization of ZsG+ cells with an increased number of 53BP1 foci by decreasing GSH contents and increasing ROS accumulation. The low proteasome activity played a role in xCT upregulation. In conclusion, canine tumor cells with low proteasome activity are radio-resistant due to high GSH content and low ROS accumulation. Sulfasalazine causes radiosensitization of the tumor cells by altering redox balance by inhibiting GSH synthesis for effective targeting of canine CSCs.
• Evaluation of the course of improvement with molnupiravir treatment for feline infectious peritonitis.
  Shino Yoshida; Mei Sugawara-Suda; Kazuyoshi Sasaoka; Noboru Sasaki; Nozomu Yokoyama; Kensuke Nakamura; Keitaro Morishita; Sangho Kim; Takafumi Sunaga; Mitsuyoshi Takiguchi
  The Canadian veterinary journal = La revue veterinaire canadienne, 66, 5, 546, 554, May 2025, [International Magazine]
  English, Scientific journal, OBJECTIVE: To clarify the clinical course during molnupiravir treatment for feline infectious peritonitis (FIP). ANIMALS AND PROCEDURE: Cats diagnosed with FIP and treated with molnupiravir at Hokkaido University Veterinary Teaching Hospital (Sapporo, Hokkaido, Japan) were retrospectively reviewed. RESULTS: Eleven cats were eligible for inclusion. Six cats had effusive FIP and 5 had non-effusive FIP. In noneffusive cases, 2 cats had neurological abnormalities at diagnosis, whereas 1 additional cat developed neurological signs during treatment. The median initial dosage of molnupiravir was 13.0 mg/kg (range: 10.0 to 15.0 mg/kg), PO, q12h. One cat died after 11 d and the remaining 10 cats completed an 84-day course of treatment. All neurological cases were given dosage increases, extended treatment duration, or both. The median final dosage of molnupiravir in non-neuro-FIP cases was 13.1 mg/kg (range: 10.0 to 15.0 mg/kg), PO, q12h, whereas dosages in neuro-FIP cases were 15.0, 15.2, and 17.2 mg/kg, PO, q12h in the 3 affected cats, respectively. In non-neurological cases, dysrexia, lethargy, and high serum amyloid A were resolved within 15 d. Total follow-up duration ranged from 175 to 362 d. No relapses were observed. CONCLUSION AND CLINICAL RELEVANCE: Monitoring responses to molnupiravir treatment requires observing clinical signs and conducting clinicopathological evaluations, including acute-phase protein evaluation.
• Enhancement of radio-sensitivity by inhibition of Janus kinase signaling with oclacitinib in canine tumor cell lines.
  Ryo Owaki; Kenji Hosoya; Tatsuya Deguchi; Satoru Konnai; Naoya Maekawa; Tomohiro Okagawa; Hironobu Yasui; Sangho Kim; Takafumi Sunaga; Masahiro Okumura
  Molecular therapy. Oncology, 33, 1, 200946, 200946, 20 Mar. 2025, [International Magazine]
  English, Scientific journal, A combination of irradiation and oclacitinib, a Janus kinase (JAK) inhibitor used in dogs, could lead to synergistic anticancer effects in canine tumors. However, the anti-tumor effects of oclacitinib remain unclear. This study investigated the radio-sensitizing effect of oclacitinib in canine tumors and determined its underlying mechanisms using osteosarcoma (HMPOS), malignant melanoma (CMeC), and thyroid adenocarcinoma (CTAC) cell lines. A clonogenic assay and a tumor growth assessment in a xenograft mouse model (BALB/cAJcl-nu/nu) were performed to evaluate the radio-sensitizing effects of oclacitinib. Oclacitinib enhanced the radio-sensitivity of tumor cells both in vitro and in vivo. The signal transducer and activator of transcription (STAT)3 expression was activated and suppressed by oclacitinib in X-irradiation-exposed cells. Oclacitinib enhanced radiation-induced apoptosis only in HMPOS cells by inhibiting anti-apoptotic genes. In addition, oclacitinib inhibited the transcription of cell-cycle-regulating genes and arrested cell cycle progression from the G1 phase to subsequent phases. In conclusion, oclacitinib enhanced radio-sensitivity both in vitro and in vivo by triggering apoptosis and impeding cell cycle progression via STAT3 inhibition in canine tumor cell lines. This study suggested the clinical therapeutic potential of oclacitinib and radiation therapy in enhancing treatment efficacy and outcomes in canine tumors.
• In vitro chondrotoxicity of bupivacaine, levobupivacaine and ropivacaine and their effects on caspase activity in cultured canine articular chondrocytes.
  Carol Mwale; Takafumi Sunaga; Yanlin Wang; Eugene C Bwalya; H M Suranji Wijekoon; Sangho Kim; Masahiro Okumura
  The Journal of veterinary medical science, 09 Mar. 2023, [Domestic magazines]
  English, Scientific journal, Bupivacaine, levobupivacaine and ropivacaine are potent, long acting, amide-type local anesthetics that have several clinical applications including intra-articular administration. The objectives of this study were to evaluate their in vitro effects on cell viability and caspase activity to elucidate whether they activate the extrinsic or intrinsic pathways of apoptosis in canine articular chondrocytes. Chondrocytes in monolayer culture were treated with culture medium as the control, or with 0.062% (0.62 mg/mL) bupivacaine, 0.062% levobupivacaine, and 0.062% ropivacaine for 24 hr. Cell viability was evaluated using the live/dead, 3-(4,5-dimehylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), and Cell Counting Kit-8 (CCK-8) assays. Evaluation of caspase-3, caspase-8, and caspase-9 activity was performed using colorimetric assays. The MTT and CCK-8 assays were used to evaluate the effect of caspase inhibitors on local anesthetic chondrotoxicity. All three local anesthetics decreased chondrocyte viability after 24 hr (P˂0.001). Apoptosis was induced through both the extrinsic and intrinsic pathways. Bupivacaine increased caspase-3, caspase-8, and caspase-9 activity (P˂0.001). Levobupivacaine increased caspase-3 (P=0.03) while ropivacaine did not significantly upregulate activity for all three caspases. Caspase inhibition did not suppress bupivacaine chondrotoxicity whereas inhibition of caspase-8 and caspase-9 decreased ropivacaine chondrotoxicity and mildly attenuated levobupivacaine chondrotoxicity. In summary, the level of chondrotoxicity, the type of caspase activated, the level of caspase activation, and the response to caspase inhibitors was dependent on the type of local anesthetic. Therefore, ropivacaine may be a safer choice for intra-articular administration compared to levobupivacaine and bupivacaine.
• In vitro chondrotoxicity of bupivacaine at low concentrations in cultured canine articular chondrocytes
  Carol Mwale; Takafumi Sunaga; Yanlin Wang; Eugene C. Bwalya; H. M; Suranji Wijekoon; Sangho Kim; Masahiro Okumura
  Japanese Journal of Veterinary Research, 71, 1, 1, 11, Mar. 2023, [Peer-reviewed]
  English, Scientific journal
• Regulation of programmed death ligand 1 expression by interferon-γ and tumour necrosis factor-α in canine tumour cell lines.
  Ryo Owaki; Tatsuya Deguchi; Satoru Konnai; Naoya Maekawa; Tomohiro Okagawa; Kenji Hosoya; Sangho Kim; Takafumi Sunaga; Masahiro Okumura
  Veterinary and comparative oncology, 21 Feb. 2023, [International Magazine]
  English, Scientific journal, Expression of programmed death ligand 1 (PD-L1) on tumor cells provides an immune evasion mechanism by inducing suppression of cytotoxic T cells. Various regulatory mechanisms of PD-L1 expression have been described in human tumours, however, little is known in canine tumours. To investigate whether inflammatory signaling is involved in PD-L1 regulation in canine tumours, the effects of interferon (IFN)-γ and tumour necrosis factor (TNF)-α treatment were examined in canine malignant melanoma cell lines (CMeC and LMeC) and an osteosarcoma cell line (HMPOS). The protein level of PD-L1 expression was upregulated by IFN-γ and TNF-α stimulation. Upon IFN-γ stimulation, all cell lines showed an increase in expression of PD-L1, signal transducer and activator of transcription (STAT)1, STAT3 and genes regulated by STAT activation. Upregulated expression of these genes was suppressed by the addition of a JAK inhibitor, oclacitinib. Contrastingly, upon TNF-α stimulation, all cell lines exhibited higher gene expression of the nuclear factor kappa B (NF-κB) gene RELA and genes regulated by NF-κB activation, whereas expression of PD-L1 was upregulated in LMeC only. Upregulated expression of these genes was suppressed by the addition of an NF-κB inhibitor, BAY 11-7082. The expression level of cell surface PD-L1 induced by IFN-γ and TNF-α treatment was reduced by oclacitinib and BAY 11-7082, respectively, indicating that upregulation of PD-L1 expression by IFN-γ and TNF-α stimulation is regulated via the JAK-STAT and NF-κB signaling pathways, respectively. These results provide insights into the role of inflammatory signaling in PD-L1 regulation in canine tumours. This article is protected by copyright. All rights reserved.
• Pentosan polysulfate sodium promotes redifferentiation to the original phenotype in micromass-cultured canine articular chondrocytes and exerts molecular weight-dependent effects
  Yanlin WANG; Takafumi SUNAGA; Carol MWALE; Ekkapol AKARAPHUTIPORN; Sangho KIM; Masahiro OKUMURA
  Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 2023
  Scientific journal
• ポリ硫酸ペントサンのPI3K/Akt経路を介したイヌ関節軟骨細胞における細胞増殖の抑止および分化度の調節に対する効果
  王 延りん; Carol Mwale; 金 尚昊; 須永 隆文; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 165回, [HS3P, 02], (公社)日本獣医学会, Sep. 2022
  Japanese
• Visualizing the cancer stem-like properties of canine tumour cells with low proteasome activity.
  Koangyong Sung; Kenji Hosoya; Yusuke Murase; Tatsuya Deguchi; Sangho Kim; Takafumi Sunaga; Masahiro Okumura
  Veterinary and comparative oncology, 20, 1, 324, 335, Mar. 2022, [International Magazine]
  English, Scientific journal, Cancer stem-like cells (CSCs) cause treatment failure in various tumours; however, establishing CSC-targeted therapies has been hampered by difficulties in the identification and isolation of this small sub-population of cells. Recent studies have revealed that tumour cells with low proteasome activity display a CSC phenotype that can be utilized to image CSCs in canines. This study visualizes and reveals the CSC-like properties of tumour cells with low proteasome activity in HMPOS (osteosarcoma) and MegTCC (transitional cell carcinoma), which are canine cell lines. The parent cells were genetically engineered to express ZsGreen1, a fluorescent protein connected to the carboxyl-terminal degron of canine ornithine decarboxylase that accumulates with low proteasome activity (ZsG+ cells). ZsG+ cells were imaged and the mode of action of this system was confirmed using a proteasome inhibitor (MG-132), which increased the ZsGreen1 fluorescence intensity. The CSC-like properties of ZsG+ cells were evaluated on the basis of cell divisions, cell cycle, the expression of CSC markers and tumourigenicity. ZsG+ cells underwent asymmetric divisions and had a low percentage of G0/G1 phase cells; moreover, ZsG+ cells expressed CSC markers such as CD133 and showed a large tumourigenic capability. In histopathological analysis, ZsG+ cells were widely distributed in the tumour samples derived from ZsG+ cells and in the proliferative regions of the tumours. The results of this study indicate that visualized canine tumour cells with low proteasome activity have a CSC-like phenotype and that this visualization system can be utilized to identify and isolate canine CSCs.
• Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses.
  Daiki Murata; Shingo Ishikawa; Takafumi Sunaga; Yasuo Saito; Takeshi Sogawa; Koichi Nakayama; Seiji Hobo; Takashi Hatazoe
  BMC veterinary research, 18, 1, 53, 53, 22 Jan. 2022, [International Magazine]
  English, Scientific journal, BACKGROUND: Medical interventions for subchondral bone cysts in horses have been extensively studied. This study investigated the regeneration of articular cartilage and subchondral bone with scaffold-free three-dimensional (3D) constructs of equine synovial membrane-derived mesenchymal stem cells (SM-MSCs) isolated from three ponies and expanded until over 1.0 × 107 cells at passage 2 (P2). RESULTS: SM-MSCs were strongly positive for CD11a/CD18, CD44, and major histocompatibility complex (MHC) class I; moderately positive for CD90, CD105, and MHC class II; and negative for CD34 and CD45 on flow cytometry and differentiated into osteogenic, chondrogenic, and adipogenic lineages in the tri-lineage differentiation assay. After culturing SM-MSCs until P3, we prepared a construct (diameter, 6.3 mm; height, 5.0 mm) comprising approximately 1920 spheroids containing 3.0 × 104 cells each. This construct was confirmed to be positive for type I collagen and negative for type II collagen, Alcian blue, and Safranin-O upon histological analysis and was subsequently implanted into an osteochondral defect (diameter, 6.8 mm; depth, 5.0 mm) at the right femoral medial condyle. The contralateral (left femoral) defect served as the control. At 3 and 6 months after surgery, the radiolucent volume (RV, mm3) of the defects was calculated based on multiplanar reconstruction of computed tomography (CT) images. Magnetic resonance (MR) images were evaluated using a modified two-dimensional MR observation of cartilage repair tissue (MOCART) grading system, while macroscopic (gross) and microscopic histological characteristics were scored according to the International Cartilage Repair Society (ICRS) scale. Compared to the control sites, the implanted defects showed lower RV percentages, better total MOCART scores, higher average gross scores, and higher average histological scores. CONCLUSIONS: Implantation of a scaffold-free 3D-construct of SM-MSCs into an osteochondral defect could regenerate the original structure of the cartilage and subchondral bone over 6 months post-surgery in horses, indicating the potential of this technique in treating equine subchondral bone cysts.
• Pentosan polysulfate regulates hepcidin expression in native Mongolian horses
  Suranji WIJEKOON; Mijiddorj TSOGBADRAKH; Takafumi SUNAGA; Yanlin WANG; Carol MWALE; Sangho KIM; Damdinsuren ALIMAA; Masahiro OKUMURA
  Journal of Veterinary Medical Science, 84, 10, 1437, 1441, Japanese Society of Veterinary Science, 2022
  Scientific journal
• Pentosan polysulfate regulates hepcidin 1-facilitated formation and function of osteoclast derived from canine bone marrow.
  Suranji Wijekoon; Takafumi Sunaga; Yanlin Wang; Carol Mwale; Sangho Kim; Masahiro Okumura
  PloS one, 17, 3, e0265596, 2022, [International Magazine]
  English, Scientific journal, Hepcidin which is the crucial regulator of iron homeostasis, produced in the liver in response to anemia, hypoxia, or inflammation. Recent studies have suggested that hepcidin and iron metabolism are involved in osteoporosis by inhibiting osteoblast function and promoting osteoclastogenesis. Pentosan polysulfate (PPS) is a heparin analogue and promising novel therapeutic for osteoarthritis (OA). This study was undertaken to determine whether PPS inhibits hepcidin-facilitated osteoclast (OC) differentiation and iron overload. Canine (n = 3) bone marrow mononuclear cells were differentiated to OC by macrophage colony-stimulating factor and receptor-activator of nuclear factor kappaB ligand with the treatment of hepcidin1 (200, 400, 800, 1200 nmol/L) and PPS (1, 5, 10, 20, 40 μg/mL). Differentiation and function of OC were accessed using tartrate-resistant acid phosphate staining and bone resorption assay while monitoring ferroportin1 (FPN1) and iron concentration by immunocytochemistry. Gene expression of OC for cathepsin K (CTK), matrix metallopeptidase-9, nuclear factor of activated-T-cells cytoplasmic 1 and FPN1 was examined. Hepcidin1 showed significant enhancement of OC number at 800 nmol/L (p<0.01). PPS impeded hepcidin-facilitated OC at 1, 5 and 10 μg/mL and reduction of resorption pits at 5 and 10 μg/mL (p< 0.01). All OC specific genes were downregulated with PPS, specifically in significant manner with CTK at higher concentrations. However, heparin induced FPN1 internalization and degradation was inhibited at higher concentrations of PPS while restoring iron-releasing capability of OC. We demonstrate for the first time that PPS is a novel-inhibitor of hepcidin-facilitated OC formation/function which might be beneficial for treatment of OA and osteoporosis.
• イヌ腫瘍細胞株におけるProgrammed cell death-ligand 1の発現機序の解析
  大脇 稜; 出口 辰弥; 今内 覚; 前川 直也; 細谷 謙次; 金 尚昊; 須永 隆文; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 164回, [HSO, 19], (公社)日本獣医学会, Sep. 2021
  Japanese
• 犬軟骨細胞における一酸化窒素産生とアポトーシスに対するペントサン多硫酸ナトリウムの抑制 分子量依存作用(Inhibition of Pentosan Polysulfate Sodium on nitric oxide production and apoptosis in Canine Chondrocytes: a molecular weight dependent effect)
  Wang Yanlin; Carol Mwale; Ekkapol Akaraphutiporn; 金 尚昊; 須永 隆文; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 164回, [HSO, 42], (公社)日本獣医学会, Sep. 2021
  English
• ブピバカインの軟骨毒性に対するペントサン多硫酸(PPS)の作用(Effects of Pentosan polysulfate(PPS) on bupivacaine chondrotoxicity)
  Carol Mwale; Wang Yanlin; Ekkapol Akaraphutiporn; Eugene Bwalya; Suranji Wijekoon; 金 尚昊; 須永 隆文; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 164回, [HSO, 45], (公社)日本獣医学会, Sep. 2021
  English
• イヌ腫瘍細胞株におけるProgrammed cell death-ligand 1の発現機序の解析
  大脇 稜; 出口 辰弥; 今内 覚; 前川 直也; 細谷 謙次; 金 尚昊; 須永 隆文; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 164回, [HSO, 19], (公社)日本獣医学会, Sep. 2021
  Japanese
• An Insight into the Role of Apoptosis and Autophagy in Nitric Oxide-Induced Articular Chondrocyte Cell Death
  Ekkapol Akaraphutiporn; Takafumi Sunaga; Eugene C. Bwalya; Wang Yanlin; Mwale Carol; Masahiro Okumura
  CARTILAGE, Dec. 2020
  English, Scientific journal
• Effects of pentosan polysulfate on cell proliferation, cell cycle progression and cyclin-dependent kinases expression in canine articular chondrocytes.
  Ekkapol Akaraphutiporn; Eugene C Bwalya; Sangho Kim; Takafumi Sunaga; Ryosuke Echigo; Masahiro Okumura
  The Journal of veterinary medical science, 82, 8, 1209, 1218, 28 Aug. 2020, [Domestic magazines]
  English, Scientific journal, Pentosan polysulfate (PPS) is a semi-synthetic sulfated polysaccharide compound which has been shown the benefits on therapeutic treatment for osteoarthritis (OA) and has been proposed as a disease modifying osteoarthritis drugs (DMOADs). This study investigated the effects of PPS on cell proliferation, particularly in cell cycle modulation and phenotype promotion of canine articular chondrocytes (AC). Canine AC were treated with PPS (0-80 µg/ml) for 24, 48 and 72 hr. The effect of PPS on cell viability, cell proliferation and cell cycle distribution were analyzed by MTT assay, DNA quantification and flow cytometry. Chondrocyte phenotype was analyzed by quantitative real-time PCR (qPCR) and glycosaminoglycan (GAG) quantification. PPS significantly reduced AC proliferation through cell cycle modulation particularly by maintaining a significantly higher proportion of chondrocytes in the G1 phase and a significantly lower proportion in the S phase of the cell cycle in a concentration- and time-dependent manner. While the proportion of chondrocytes in G1 phase corresponded with the significant downregulation of cyclin-dependent kinase (CDK) 1 and 4. Furthermore, the study confirms that PPS promotes a chondrogenic phenotype of AC through significant upregulation of collagen type II (Col2A1) mRNA and GAG synthesis. The effect of PPS on the inhibition of chondrocyte proliferation while promoting a chondrocyte phenotype could be beneficial in the early stages of OA treatment, which transient increase in proliferative activity of chondrocytes with subsequent phenotypic shift and less productive in an essential component of extracellular matrix (ECM) is observed.
• Clinical evaluation of pentosan polysulfate as a chondroprotective substance in native Mongolian horses
  Mijiddorj Tsogbadrakh; Takafumi Sunaga; Eugene Bwalya; Suranji Wijekoon; Ekkapol Akaraphutiporn; Yanlin Wang; Carol Mwale; Adiya Naranbaatar; Sangho Kim; Kenji Hosoya; Damdinsuren Alimaa; Masahiro Okumura
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 68, 3, 203, 208, Aug. 2020
  English, Scientific journal
• Alterations in characteristics of canine articular chondrocytes in non-passaged long-term monolayer culture: Matter of differentiation, dedifferentiation and redifferentiation.
  Ekkapol Akaraphutiporn; Takafumi Sunaga; Eugene C Bwalya; Ryosuke Echigo; Masahiro Okumura
  The Journal of veterinary medical science, 82, 6, 793, 803, 24 Jun. 2020, [Domestic magazines]
  English, Scientific journal, This study investigated the effects of culture time on phenotype stability of canine articular chondrocytes (CACs) in non-passaged long-term monolayer culture. Third passage (P3) CACs isolated from four cartilage samples were seeded at three different initial seeding densities (0.2 × 104, 1.0 × 104 and 5.0 × 104 cells/cm2) and maintained in monolayer condition up to 8 weeks without undergoing subculture after confluence. The characteristic changes of chondrocytes during the culture period were evaluated based on the cell morphology, cell proliferation, glycosaminoglycans (GAGs) content, DNA quantification, mRNA expression and ultrastructure of chondrocytes. Chondrocytes maintained under post-confluence condition exhibited a capability to grow and proliferate up to 4 weeks. Alcian blue staining and Dimethylmethylene blue (DMMB) assay revealed that the extracellular matrix (ECM) synthesis was increased in a time-dependent manner from 2 to 8 weeks. The chondrocyte mRNA expression profile was dramatically affected by prolonged culture time, with a significant downregulation of collagen type I, whereas the expression of collagen type II, aggrecan, Sox9 and matrix metalloproteinase 13 (MMP-13) were significantly upregulated. In addition, transmission electron microscopy (TEM) result indicated dilation of rough endoplasmic reticulum (RER) in these long-term monolayer cultured chondrocytes. These findings demonstrate that the chondrocytes phenotype could be partially redifferentiated through the spontaneous redifferentiation process in long-term cultures using standard culture medium without the addition of chondrogenic supplements or tissue-culture scaffolds.
• 半導体レーザ焼灼により治療を試みた黄色腫の猫1例
  山口 亮; 金 尚昊; 桜井 雄平; 細谷 謙次; 須永 隆文; 奥村 正裕
  北海道獣医師会雑誌, 63, 8, 347, 347, (公社)北海道獣医師会, Aug. 2019
  Japanese
• Osteochondral regeneration using constructs of mesenchymal stem cells made by bio three-dimensional printing in mini-pigs.
  Atsushi Yamasaki; Yoshihiro Kunitomi; Daiki Murata; Takafumi Sunaga; Tomohide Kuramoto; Takeshi Sogawa; Kazuhiro Misumi
  Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37, 6, 1398, 1408, Jun. 2019, [International Magazine]
  English, Scientific journal, Osteoarthritis is a major joint disease that has been extensively investigated in humans and in model animals. In this study, we examined the regeneration of articular cartilage and subchondral bone using artificial scaffold-free constructs composed of adipose tissue-derived mesenchymal stem cells (AT-MSCs) created using bio three-dimensional (3D) printing with a needle-array. Printed constructs were implanted into osteochondral defects created in the right femoral trochlear groove of six mini-pigs, using femoral defects created in the left femurs as controls. Repair within the defects was evaluated at 3 and 6 months post-implantation using computed tomography (CT) and magnetic resonance (MR) imaging. The radiolucent volume (RV, mm3 ) in the defects was calculated using multi-planar reconstruction of CT images. MR images were evaluated based on a modified 2D- MOCART (magnetic resonance observation of cartilage repair tissue) grading system. Gross and microscopic pathology were scored according to the ICRS (International Cartilage Repair Society) scale at 6 months after implantation. The percentage RV at 3 months postoperation was significantly lower in the implanted defects than in the controls, whereas total scores based on the MOCART system were significantly higher in the implanted defects as compared with the controls. Although there were no statistical differences in the gross scores, the average histological scores were significantly higher in the implanted defects than in the controls. To our knowledge, this is the first report to suggest that artificial scaffold-free 3D-printed constructs of autologous AT-MSCs can be aid in the osteochondral regeneration in pigs. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1398-1408, 2019.
• A pilot study of regenerative therapy by implanting synovium-derived mesenchymal stromal cells in equine osteochondral defect models.
  Atsushi Yamasaki; Takaya Omura; Daiki Murata; Minoru Kobayashi; Takafumi Sunaga; Kanichi Kusano; Yoshiharu Ueno; Tomohide Kuramoto; Seiji Hobo; Kazuhiro Misumi
  Journal of equine science, 29, 4, 117, 122, Dec. 2018, [Domestic magazines]
  English, Scientific journal, Synovium-derived mesenchymal stromal cells (SM-MSCs) from seven Thoroughbreds with naturally occurring intra-articular fracture proliferated to over ten million cells by the second passage. Using three experimental Thoroughbreds, columnar osteochondral defects were made arthroscopically at the bilateral distal radius. Five million allogenic SM-MSCs were implanted into the right defect, and another five million were injected into the right radio-carpal joint (implantation site). No SM-MSCs were implanted into the left defect or the same joint (control site). At 3 and 6 weeks after surgery, ten million autologous SM-MSCs were injected into the right joints. Radiolucent volumes of defects calculated by analysis of postmortem CT images 9 weeks after surgery were decreased in implanted sites compared with control sites in all horses. The average scores for ICRS gross and histopathological grading scales in implanted sites were equal to or higher than those of the controls. These results suggest that allogenic implantation and subsequent autologous injection of SM-MSCs might not obstruct subchondral bone formation in defects.
• ウマ滑膜由来間葉系幹細胞を用いて作製した立体構造体による膝関節荷重面の骨軟骨再生
  村田 大紀; 石川 真悟; 齋藤 靖生; 須永 隆文; 十川 英; 藤木 誠; 三角 一浩; 中山 功一; 帆保 誠二; 畠添 孝
  日本獣医学会学術集会講演要旨集, 161回, 419, 419, (公社)日本獣医学会, Aug. 2018
  Japanese
• Aberrant expression of microRNAs and the miR-1/MET pathway in canine hepatocellular carcinoma.
  Y-C Lai; N Ushio; M M Rahman; Y Katanoda; K Ogihara; Y Naya; A Moriyama; T Iwanaga; Y Saitoh; T Sogawa; T Sunaga; Y Momoi; H Izumi; N Miyoshi; Y Endo; M Fujiki; H Kawaguchi; N Miura
  Veterinary and comparative oncology, 16, 2, 288, 296, Jun. 2018, [International Magazine]
  English, Scientific journal, Canine hepatocellular carcinoma (HCC) is the most common primary hepatic tumour in dogs. MicroRNA (miRNA) dysregulation has been reported in human HCC and shown to have diagnostic and prognostic value; however, there are no data on miRNA expression in canine HCC. The aim of the present study was to investigate differentially expressed miRNAs in canine HCC. Analysis of miRNA expression in canine HCC tissues and cell lines by quantitative reverse transcription PCR showed that miR-1, miR-122, let-7a, and let-7g were downregulated, whereas miR-10b and miR-21 were upregulated in canine HCC. MET is one of the target genes of miR-1. MET was upregulated in canine HCC at the gene and protein levels, and a significant correlation between the concomitant downregulation of miR-1 and upregulation of MET was observed. Fast/intermediate-proliferating canine HCC cell lines had higher MET gene and protein expression levels than the slow-proliferating cell line. These findings suggest that miRNAs are differentially expressed in canine HCC, and that the miR-1/MET pathway may be associated with canine HCC cell proliferation.
• Characteristics and multipotency of equine dedifferentiated fat cells.
  Daiki Murata; Atsushi Yamasaki; Shouta Matsuzaki; Takafumi Sunaga; Makoto Fujiki; Satoshi Tokunaga; Kazuhiro Misumi
  Journal of equine science, 27, 2, 57, 65, 2016, [Domestic magazines]
  English, Scientific journal, Dedifferentiated fat (DFAT) cells have been shown to be multipotent, similar to mesenchymal stem cells (MSCs). In this study, we aimed to establish and characterize equine DFAT cells. Equine adipocytes were ceiling cultured, and then dedifferentiated into DFAT cells by the seventh day of culture. The number of DFAT cells was increased to over 10 million by the fourth passage. Flow cytometry of DFAT cells showed that the cells were strongly positive for CD44, CD90, and major histocompatibility complex (MHC) class I; moderately positive for CD11a/18, CD105, and MHC class II; and negative for CD34 and CD45. Moreover, DFAT cells were positive for the expression of sex determining region Y-box 2 as a marker of multipotency. Finally, we found that DFAT cells could differentiate into osteogenic, chondrogenic, and adipogenic lineages under specific nutrient conditions. Thus, DFAT cells could have clinical applications in tissue regeneration, similar to MSCs derived from adipose tissue.
• Limited inhibitory effects of non-steroidal antiinflammatory drugs on in vitro osteogenic differentiation in canine cells.
  Namgil Oh; Takafumi Sunaga; Hiroki Yamazaki; Kenji Hosoya; Satoshi Takagi; Masahiro Okumura
  The Japanese journal of veterinary research, 61, 3, 97, 107, Aug. 2013, [Domestic magazines]
  English, Scientific journal, Cyclooxygenase (COX)-2 participates essentially in bone healing, demonstrated by COX-2 knockout mice that showed delayed fracture repair. Considerable controversy still exists on inhibitory effects of COX-2 inhibitors on bone healing in clinical cases. To assess stage-dependent effects of short-term treatment of COX-2 inhibitors on osteogenic differentiation, a canine POS osteosarcoma cell line which spontaneously differentiates into osteoblastic cell was exposed to COX-2 inhibitors such as carprofen and meloxicam for 72 hours during three different stages of osteoblast differentiation, including day 0 to 3 (pre-osteoblastic stage), day 4 to 7 (transitional stage) and day 8 to 11 (mature osteoblastic stage). As osteogenic markers, expression of alkaline phosphatase (ALP) was estimated by analysis of mRNA expression, enzymatic activity and ALP staining, and expression of osteocalcin was estimated by analysis of mRNA expression after the drug treatments. Calcified matrix formation was finally observed by von Kossa staining on day 14. Expressions of ALP showed no significant suppression by carprofen and meloxicam during all three stages. However, expressions of osteocalcin mRNA and non-calcified nodule formations were delayed by carprofen and meloxicam during transitional stage. Nevertheless, fully calcified nodule formation was observed in all experimental groups during post-medication period. These results indicate that short-term treatment of carprofen and meloxicam would reversibly suppress the differentiation of osteoblasts.
• Inhibitory effects of pentosan polysulfate sodium on MAP-kinase pathway and NF-κB nuclear translocation in canine chondrocytes in vitro.
  Takafumi Sunaga; Namgil Oh; Kenji Hosoya; Satoshi Takagi; Masahiro Okumura
  The Journal of veterinary medical science, 74, 6, 707, 11, Jun. 2012, [Domestic magazines]
  English, Scientific journal, Pentosan polysulfate sodium (PPS) has a heparin-like structure and is purificated from the plant of European beech wood. PPS has been used for the treatment of interstitial cystitis for human patients. Recent years, it was newly recognised that PPS reduce pain and inflammation of OA. The molecular biological mechanism of PPS to express its clinical effects is not fully understood. The purpose of the present study is to investigate a mechanism of action of PPS on inflammatory reaction of chondrocytes in vitro. It was evaluated that effects of PPS on interleukin (IL)-1β-induced phosphorylation of mitogen-actiated protein kinases (MAPKs), such as p38, extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), nuclear translocation of nuclear factor-kappa B (NF-κB), and matrix metalloproteinase (MMP)-3 production in cultured articular chondrocytes. As a result, in the presence of PPS existence, IL-1β-induced phosphorylation of p38 and ERK were certainly inhibited, while JNK phosphorylation was not affected. Nuclear translocation of NF-κB and MMP-3 production were suppressed by PPS pretreatment prior to IL-1β stimulation. In conclusion, it is strongly suggested that PPS treatment prevents inflammatory intracellular responses induced by IL-1 β through inhibition of phosphorylation of certain MAPKs, p38 and ERK and then nuclear translocation of NF-κB in cultured chondrocytes. These PPS properties may contribute to suppressive consequence of catabolic MMP-3 synthesis. These data might translate the clinical efficacy as PPS treatment could inhibit the cartilage catabolism and related clinical symptoms of OA in dogs.
• Pro-apoptotic effects of tepoxalin, a cyclooxygenase/lipoxygenase dual inhibitor, on canine synovial fibroblasts.
  Takafumi Sunaga; Namgil Oh; Kenji Hosoya; Satoshi Takagi; Masahiro Okumura
  The Journal of veterinary medical science, 74, 6, 745, 50, Jun. 2012, [Domestic magazines]
  English, Scientific journal, Canine osteoarthritis occurs frequently and causes secondary synovitis. Administration of nonsteroidal anti-inflammatory drugs (NSAIDs) is one of the major therapeutic options for pain management of joint diseases. Tepoxalin has an unique property as an NSAIDs that suppresses both cyclooxygenase and lipoxygenase. The purpose of this study was to evaluate antiproliferative effects of tepoxalin on cultured canine synovial cells. Cytotoxic effects of tepoxalin, carprofen, meloxicam and AA-861 on cultured canine synoviocytes were evaluated by MTT colorimetric assay. Apoptosis was detected by morphological observations with Giemsa or annexin V/Hoechst 33342 staining and by the inhibition of caspase-3 activity with N-Ac-Asp-Glu-Val-Asp-CHO (Ac-DEVD-CHO). Cytotoxic effects of tepoxalin were evident in comparison with the effects of carprofen or meloxicam. The same tendency of cytotoxicity was observed when 5-lipoxygenase was inhibited by AA-861. The morphological findings and contradictory effects of Ac-DEVD-CHO with regard to the cytotoxicity proved the proapoptotic effects of tepoxalin. In conclusion, tepoxalin might control osteoarthritic synovitis by inducing apoptosis in proliferating synoviocytes, while most NSAIDs that selectively inhibit cyclooxygenase-2 most likely would not suppress synovial proliferation.
• 柴犬の膝蓋骨内方脱臼における後肢骨格変形の画像的特徴
  青木 由徳; 須永 隆文; 山崎 裕毅; 細谷 謙次; 高木 哲; 奥村 正裕
  北海道獣医師会雑誌, 55, 8, 401, 401, (公社)北海道獣医師会, Aug. 2011
  Japanese
• 犬関節軟骨細胞における外因性ヒアルロン酸のヒアルロン酸合成酵素遺伝子発現に対する効果
  須永 隆文; 呉 南佶; 山崎 裕毅; 足立 真実; 細谷 謙次; 高木 哲; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 152回, 294, 294, (公社)日本獣医学会, Aug. 2011
  Japanese
• 犬の組織球肉腫におけるSurvivin遺伝子発現の予後因子としての評価
  山崎 裕毅; 高木 哲; 呉 南佶; 須永 隆文; 細谷 謙次; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 152回, 302, 302, (公社)日本獣医学会, Aug. 2011
  Japanese
• 犬の骨芽細胞分化および成熟における非ステロイド性抗炎症薬の影響
  呉 南佶; 須永 隆文; 山崎 裕毅; 細谷 謙次; 高木 哲; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 151回, 186, 186, (公社)日本獣医学会, Mar. 2011
  Japanese
• 犬培養滑膜細胞に対するテポキサリンのアポトーシス誘導作用と細胞内シグナル伝達系への関与
  須永 隆文; 細谷 謙次; 高木 哲; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 150回, 304, 304, (公社)日本獣医学会, Sep. 2010
  Japanese
• 腸瘻チューブによる栄養管理を実施した犬猫15症例の検討
  山崎 裕毅; 高木 哲; 須永 隆文; 青木 由徳; 細谷 謙次; 奥村 正裕
  北海道獣医師会雑誌, 54, 8, 466, 466, (公社)北海道獣医師会, Aug. 2010
  Japanese
• 血管拡張型骨肉腫と診断された犬の3症例
  青木 由徳; 高木 哲; 細谷 謙次; 須永 隆文; 大崎 智弘; 落合 謙爾; 梅村 孝司; 奥村 正裕
  獣医麻酔外科学雑誌, 41, Suppl.1, 332, 332, (一社)日本獣医麻酔外科学会, Jul. 2010
  Japanese
• 培養馬滑膜および軟骨細胞における多硫酸化グリコサミノグリカンの抗炎症効果発現機序
  住江 康晴; 須永 隆文; 光田 健太; 金 尚昊; 細谷 謙次; 高木 哲; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 149回, 286, 286, (公社)日本獣医学会, Mar. 2010
  Japanese
• 犬培養滑膜細胞に対するリポキシゲナーゼ阻害薬AA861のMAPK経路への効果およびアポトーシス誘導作用
  須永 隆文; 住江 康晴; 細谷 謙次; 高木 哲; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 149回, 288, 288, (公社)日本獣医学会, Mar. 2010
  Japanese
• 光感受性物質Benzoporphyrin derivative monoacid ring A(BPD-MA)腫瘍組織内投与における体組織分布
  王 禎祥; 高木 哲; 大崎 智弘; 須永 隆文; 奥村 正裕
  獣医麻酔外科学雑誌, 40, Suppl.2, 197, 197, (一社)日本獣医麻酔外科学会, Dec. 2009
  Japanese
• 交通事故による膀胱・尿道損傷により尿路変更を行った犬の1例
  高木 哲; 須永 隆文; 小儀 直子; 青木 由徳; 寺本 英司; 大崎 智弘; 奥村 正裕
  動物臨床医学会年次大会プロシーディング, 30回, 2, 163, 164, 動物臨床医学会, Nov. 2009
  Japanese
• 腸間膜リンパ節から胸管造影を行った乳び胸の犬の1例
  小儀 直子; 高木 哲; 井上 愛香; 須永 隆文; 大崎 智弘; 奥村 正裕
  北海道獣医師会雑誌, 53, 8, 88, 88, (公社)北海道獣医師会, Aug. 2009
  Japanese
• 交通事故による尿道断裂に対して膀胱全摘出および尿路再建術を実施した犬1症例
  須永 隆文; 高木 哲; 小儀 直子; 青木 由徳; 寺本 英司; 大崎 智弘; 奥村 正裕
  北海道獣医師会雑誌, 53, 8, 91, 91, (公社)北海道獣医師会, Aug. 2009
  Japanese
• 胆嚢粘液嚢腫の犬5例における周術期管理
  光田 健太; 高木 哲; 須永 隆文; 滝口 満喜; 大田 寛; 佐藤 耕太; 大崎 智弘; 奥村 正裕
  北海道獣医師会雑誌, 53, 8, 93, 93, (公社)北海道獣医師会, Aug. 2009
  Japanese
• 副腎皮質機能低下症の犬2症例に対する周術期管理
  住江 康晴; 高木 哲; 須永 隆文; 青木 由徳; 千葉 依里; 久万田 剛; 千石 和彦; 奥村 正裕
  北海道獣医師会雑誌, 53, 8, 94, 94, (公社)北海道獣医師会, Aug. 2009
  Japanese
• 副腎皮質機能低下症の犬2例における周術期管理
  高木 哲; 須永 隆文; 青木 由徳; 久万田 剛; 千石 和彦; 奥村 正裕
  獣医麻酔外科学雑誌, 40, Suppl.1, 177, 177, (一社)日本獣医麻酔外科学会, Jun. 2009
  Japanese
• 長期間経過した犬の外傷性横隔膜ヘルニアの1手術例
  高木 哲; 須永 隆文; 山田 佳代子; 青木 由徳; 大崎 智弘; 奥村 正裕
  動物臨床医学, 18, 1, 11, 15, 動物臨床医学会, Mar. 2009
  Japanese
• 獣医学における腫瘍研究最前線 活性化リンパ球療法および樹状細胞ワクチン療法の臨床応用
  高木 哲; 星野 有希; 須永 隆文; 大崎 智弘; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 147回, 163, 163, (公社)日本獣医学会, Mar. 2009
  Japanese
• 犬の自然発生悪性黒色腫における腫瘍関連抗原gp100の発現とそれを用いたペプチドワクチンの免疫学的効果
  山田 佳代子; 高木 哲; 須永 隆文; 奥村 正裕
  日本獣医学会学術集会講演要旨集, 147回, 272, 272, (公社)日本獣医学会, Mar. 2009
  Japanese
• 骨密度低下による多発性骨折を認めた若齢犬の1例
  高木 哲; 須永 隆文; 星野 有希; 唯野 剛史; 梅村 孝司; 大崎 智弘; 寺本 英司; 奥村 正裕
  獣医麻酔外科学雑誌, 39, 3-4, 51, 56, (一社)日本獣医麻酔外科学会, Jan. 2009
  Japanese
• 著しい骨脆弱を認めた若齢犬の1例
  高木 哲; 寺本 英司; 須永 隆文; 大崎 智弘; 奥村 正裕
  動物臨床医学会年次大会プロシーディング, 29回, 2, 251, 252, 動物臨床医学会, Nov. 2008
  Japanese
• 犬培養滑膜細胞におけるリポキシゲナーゼ、RhoおよびプロスタグランジンE2合成酵素遺伝子検出法の最適化
  須永 隆文; 奥村 正裕; 高木 哲
  日本獣医学会学術集会講演要旨集, 146回, 264, 264, (公社)日本獣医学会, Sep. 2008
  Japanese
• 生前診断が困難であった血管肉腫の犬の1例
  須永 隆文; 高木 哲; 嶋本 良則; 梅村 孝司; 奥村 正裕
  北海道獣医師会雑誌, 52, 8, 414, 414, (公社)北海道獣医師会, Aug. 2008
  Japanese
• 肝細胞癌摘出後に多発性転移を認めた犬の2例
  高木 哲; 山崎 真大; 須永 隆文; 小川 修治; 落合 謙爾; 梅村 孝司; 奥村 正裕
  北海道獣医師会雑誌, 52, 8, 416, 416, (公社)北海道獣医師会, Aug. 2008
  Japanese
• 外傷性横隔膜ヘルニアを呈した犬の1例
  山田 佳代子; 高木 哲; 須永 隆文; 青木 由徳; 本田 英隆; 奥村 正裕
  北海道獣医師会雑誌, 52, 8, 419, 419, (公社)北海道獣医師会, Aug. 2008
  Japanese
• In vitro犬関節滑膜および軟骨細胞における外因性ヒアルロン酸および多硫酸化グリコサミノグリカンのヒアルロン酸産生効果
  溝部 文彬; 奥村 正裕; 須永 隆文; 高木 哲; 藤永 徹
  日本獣医学会学術集会講演要旨集, 145回, 216, 216, (公社)日本獣医学会, Mar. 2008
  Japanese
• 犬培養滑膜線維芽細胞に対するリポキシゲナーゼ(LOX)阻害薬のアポトーシス誘導作用
  須永 隆文; 奥村 正裕; 溝部 文彬; 田崎 竜也; 星野 有希; 高木 哲; 藤永 徹
  日本獣医学会学術集会講演要旨集, 144回, 158, 158, (公社)日本獣医学会, Aug. 2007
  Japanese
• 脊髄圧迫に関連した硬膜病変を呈した硬膜外脊髄脂肪腫の犬1症例
  奥村 正裕; 須永 隆文; 田崎 竜也; 星野 有希; 梅村 孝司; 高木 哲; 藤永 徹
  動物臨床医学会年次大会プロシーディング, 27回, 2, 87, 88, 動物臨床医学会, Nov. 2006
  Japanese
• 脊髄硬膜内外の病変により後肢起立不能を発症した犬の2症例
  須永 隆文; 奥村 正裕; 星野 有希; 梅村 孝司; 田崎 竜也; 高木 哲; 藤永 徹
  北海道獣医師会雑誌, 50, 8, 93, 93, (公社)北海道獣医師会, Aug. 2006
  Japanese
• 変形癒合のみられた大腿骨分筋骨折にインターロッキングネイル固定法(I.I.N.法)を適用した犬の1症例
  小松 広宗; 奥村 正裕; 大崎 智弘; 譽田 由香; 須永 隆文; 高木 哲; 藤永 徹
  北海道獣医師会雑誌, 50, 8, 95, 95, (公社)北海道獣医師会, Aug. 2006
  Japanese

• 意識障害は起源不明髄膜脳脊髄炎(MUO)の予後不良に関連する MUO82例の回顧的検討(2014～2022)
  笹岡 一慶; 福家 知樹; 須永 隆文; 大田 寛; 滝口 満喜, 北海道獣医師会雑誌, 68, 8, 313, 313, Aug. 2024
  (公社)北海道獣医師会, Japanese
• 重度の脊髄内出血を伴う椎間板ヘルニアが認められた猫の1例
  須永隆文; 三木信悟; 青島圭佑; 奥村正裕, 日本獣医麻酔外科学会学術集会抄録集(Web), 105th, 2022
• 犬の口腔内悪性黒色腫に対する抗Programmed death-Ligand 1抗体療法と放射線治療におけるアブスコパル効果の誘導解析
  大脇稜; 出口辰弥; 細谷謙次; 今内覚; 前川直也; 立花由莉加; 中村基司; 金尚昊; 須永隆文; 奥村正裕, 北海道獣医師会雑誌, 65, 8, 2021
• プロテアソーム活性を用いて可視化したイヌ腫細胞株由来Cancer stem-like cellsの解析
  SUNG Koangyong; 細谷謙次; 村瀬優介; 出口辰弥; 金尚昊; 須永隆文; 奥村正裕, 日本獣医学会学術集会講演要旨集, 163rd, 2020
• ロジックで学ぶ 犬と猫の臨床テクニック(第22回) 皮膚のデブライドメント
  須永 隆文, CAP: Companion Animal Practice, 34, 9, 132, 135, Sep. 2019
  (株)緑書房, Japanese
• 滅菌と消毒 手術部位感染症を予防するために
  須永 隆文, 日本獣医麻酔外科学雑誌, 50, Suppl.1, 66, 67, Jun. 2019
  (一社)日本獣医麻酔外科学会, Japanese
• 【実践 創傷管理】咬傷、刺し傷などの処置 深い傷のある患部の治療と管理
  須永 隆文, CLINIC NOTE, 13, 2, 8, 15, Feb. 2017
  (株)エデュワードプレス, Japanese
• 症例から学ぶ鑑別診断(第29回) 吐出を呈した症例
  須永 隆文, SA Medicine, 18, 2, 54, 60, Apr. 2016
  (株)エデュワードプレス, Japanese
• 四肢及び尾部の広範囲皮膚欠損創に人工真皮と遊離皮膚移植による皮膚再建を実施した犬の4例
  須永 隆文; 高木 哲; 小川 修治; 細谷 謙次; 奥村 正裕, 日本獣医師会雑誌, 66, 1, 57, 60, Jan. 2013
  (公社)日本獣医師会, Japanese
• 表面抗原解析による遺伝子診断が有用であった肺組織球肉腫の犬5例
  山崎裕毅; 高木哲; 須永隆文; 細谷謙次; 奥村正裕, 日本獣医師会雑誌, 65, 5, 2012
• Urethral Dilation Using Balloon Catheters for Uroschesis Caused by Urine Based Tumors in Four Dogs
  山崎裕毅; 高木哲; 小儀直子; 須永隆文; 青木由徳; 細谷謙次; 奥村正裕, 日本獣医師会雑誌, 65, 7, 2012
• 犬培養軟骨細胞におけるポリ硫酸ペントサンナトリウムはMAPキナーゼ経路を介しnuclear dactor-kappa Bを抑制する
  須永隆文; OH Namgil; 住江康晴; 細谷謙次; 高木哲; 奥村正裕, 日本軟骨代謝学会プログラム・抄録集, 25th, 2012
• 犬関節症における関節内投与ヒアルロン酸の治療効果とその発現機構
  奥村正裕; 須永隆文; 青木由徳; 細谷謙次; 高木哲, 日本獣医師会三学会年次大会講演要旨集, 2009, 2010
• 犬の皮膚欠損に対する遊離皮膚移植における人工真皮の有用性
  高木哲; 青木由徳; 須永隆文; 大崎智弘; 細谷謙次; 奥村正裕, 日本獣医師会三学会年次大会講演要旨集, 2009, 2010
• 血管拡張型骨肉腫と診断された犬の3症例
  青木由徳; 高木哲; 須永隆文; 大崎智弘; 寸田祐嗣; 落合謙爾; 梅村孝司; 奥村正裕, 北海道獣医師会雑誌, 53, 8, 2009

• 伴侶動物獣医療実習, 2024年, 学士課程, 獣医学部
• 最新獣医外科学特論, 2024年, 博士後期課程, 獣医学院
• プレクリニカル実習, 2024年, 学士課程, 獣医学部
• 臨床疾病学特論, 2024年, 博士後期課程, 獣医学院
• 伴侶動物獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 総合専門臨床特論, 2024年, 博士後期課程, 獣医学院
• 伴侶動物獣医療実習Ⅱ, 2024年, 学士課程, 獣医学部
• 整形外科学, 2024年, 学士課程, 獣医学部
• 夜間・救急獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• アドバンスト演習, 2024年, 学士課程, 獣医学部
• 総合獣医療実習, 2024年, 学士課程, 獣医学部
• アドバンスト演習, 2024年, 学士課程, 獣医学部
• 外科学総論, 2024年, 学士課程, 獣医学部
• 獣医コミュニケーション演習, 2024年, 学士課程, 獣医学部
• 伴侶動物獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 伴侶動物獣医療実習Ⅱ, 2024年, 学士課程, 獣医学部
• 夜間・救急獣医療実習Ⅰ, 2024年, 学士課程, 獣医学部
• 総合獣医療実習, 2024年, 学士課程, 獣医学部
• プレクリニカル実習, 2024年, 学士課程, 獣医学部

• 日本軟骨代謝学会
• 日本獣医麻酔外科学会

• Development of Jawbone Regeneration Therapy Incorporating Soft Tissue Management Utilizing High-Performance Scaffolds.
  Grants-in-Aid for Scientific Research
  01 Apr. 2023 - 31 Mar. 2027
  大久保 直登; 北川 善政; 大西 俊介; 須永 隆文
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23K27786
• Osteochondral regeneration using allogenic implantation of somatic stem cell
  Grants-in-Aid for Scientific Research
  01 Apr. 2015 - 31 Mar. 2018
  MISUMI Kazuhiro
  Regeneration of articular cartilage and subchondral bone using scaffold-free constructs composed of adipose tissue-derived mesenchymal stem cells (AT-MSCs) using bio three-dimensional (3D) printer was evaluated in minipigs. Osteochondral defects created in medial chondyles of femurs were implanted by autologous construct (autologous implantation), allogenic construct (allogenic implantation), and no construct (controls). Post-surgical computed tomography demonstrated the delayed regeneration of subchondral bone in allogenic implantation, since the radiolucent volume of defects significantly decreased in the autologous implantation comparing to allogenic implantation. Also, total scores of either the magnetic resonance image or histopathology were significantly higher in the autologous implantation than the allogenic implantation. Allogenic implantation of scaffold-free 3D-constructs of AT-MSCs could delay the regeneration in osteochondral defect comparing to autologous implantation.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Kagoshima University, 15H04600