Researcher basic information

• 博士（水産科学）, 北海道大学

• https://researchmap.jp/fbeppu?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201801007026532380

• 201801007026532380

• Lipid biochemisty
• Molecular nutrition
• Lipid metabolism
• Functional lipids
• Marine lipids
• phospholipids
• Ether lipids
• Diabetes
• Obesity
• Skeletal muscle
• ω3 Polyunsaturated fatty acids
• Vitamin E

• Life Science, Food sciences, 脂質生化学 / 分子栄養学
• Life Science, Aquatic life science, Fisheries Sciences
• Life Science, Aquatic life science, 水産脂質化学

• Bachelor's degree program, School of Fisheries Sciences
• Master's degree program, Graduate School of Fisheries Sciences
• Doctoral (PhD) degree program, Graduate School of Fisheries Sciences

Research activity information

• 2018, 日本油化学会, ベストオーサー賞
  別府 史章

• Potential role of COUP-TF in regulating major yolk protein gene expression in sea urchin.
  Ichiro Higuchi; Shiori Tsue; Yudai Kitano; Osamu Nishimiya; Masashi Hosokawa; Fumiaki Beppu; Kazuhiro Ura; Yasuaki Takagi
  General and comparative endocrinology, 376, 114867, 114867, 06 Dec. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Major yolk protein (MYP) serves as a critical nutrient reservoir for gonadal development in sea urchins, but the transcriptional regulation of its encoding gene remains largely uncharacterized. Here, we cloned and analyzed the MYP promoter from Mesocentrotus nudus and found two conserved elements with putative binding sites for the orphan nuclear receptor COUP-TF (chicken ovalbumin upstream promoter-transcription factor). We cloned the full-length cDNA encoding COUP-TF from M. nudus (MnCOUP-TF). The cDNA encoded a 472-amino acid protein highly homologous to other COUP-TF/SVP subfamily members. RT-PCR and qPCR analyses revealed that transcripts of both MnCOUP-TF and MYP were present in several tissues, including gonads, and their levels exhibited a significant positive correlation during gonadal maturation (R2 = 0.6764, p < 0.01). Electrophoretic mobility shift assays demonstrated specific binding of MnCOUP-TF to the MYP promoter, and transactivation assays showed that MnCOUP-TF activates transcription through these sites. Furthermore, total lipids extracted from gonadal tissue enhanced MnCOUP-TF-dependent reporter activity in a dose-dependent manner. This enhancement was comparable to the effect of 9-cis-retinoic acid, a compound known to activate COUP-TF at high concentrations, although it is not considered to be a physiological COUP-TF ligand. These results suggest that MnCOUP-TF regulates MYP expression in a ligand-dependent manner and that endogenous activators may be present in gonadal lipids. To our knowledge, this is the first molecular evidence implicating COUP-TF in yolk protein gene regulation in sea urchins, providing new insights into nuclear receptor-mediated control of reproduction in marine invertebrates.
• Structural determination of the carotenoid glycoside ester produced by the marine bacterium Exiguobacterium sp. strain oki7 and its antioxidant and anti-inflammatory effects
  Naoki Takatani; Takashi Maoka; Misaki Aoike; Munekimi Nanao; Tomoo Sawabe; Fumiaki Beppu; Masashi Hosokawa
  Fisheries Science, 91, 5, 999, 1011, Springer Science and Business Media LLC, 01 Jul. 2025
  Scientific journal
• Identification of flexixanthin and its derivatives in Algoriphagus bacteria and evaluation of their antioxidant and anti-inflammatory effects
  Naoki Takatani; Yuki Sato-Takabe; Misaki Aoike; Rika Sekine; Takashi Maoka; Tomoo Sawabe; Fumiaki Beppu; Kazutoshi Shindo; Masashi Hosokawa
  Food Bioscience, 65, Mar. 2025, [Peer-reviewed]
  Scientific journal
• Alkylglycerol enhances myogenesis and regulates ether-phospholipid metabolism in C2C12 myoblasts.
  Takero Sasaki; Miu Kameyama; Naoki Takatani; Masashi Hosokawa; Fumiaki Beppu
  Biochemical and biophysical research communications, 754, 151532, 151532, 23 Feb. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, 1-O-Alkylglycerol (AKG), a lipid characteristic of marine organisms, possesses an ether-linked alkyl chain on its glycerol backbone. AKG exhibits various biological activities, including anti-cancer effects, promoting sperm motility, and stimulating immune response. Metabolically, AKG is converted into alkyl- and alkenyl-phospholipids (PLs), which are key components of the cell membrane and play essential roles in maintaining membrane homeostasis and cellular functions. However, the influence of AKG on myogenesis and ether-type PL metabolism in muscle cells remains unknown. This study evaluated the effects of AKG on myogenic differentiation and ether-PL metabolism in mouse C2C12 myoblasts. During differentiation, cells were treated with 10-20 μM 1-O-octadecyl-glycerol (batyl alcohol) and 1-O-hexadecyl-glycerol (chimyl alcohol). By day 7 of differentiation, myotube size had increased in cells treated with AKGs. Comparative tests using compounds with similar or partial structures, including monoacylglycerol and alkenylglycerol, demonstrated that this activity was linked to the structural features of AKG. Conversely, myotube growth was insufficient after treatment with 1-O-dodecyl-glycerol, which contains a shorter alkyl chain. Additionally, batyl alcohol treatment elevated the levels of ether-phosphatidylcholine (PC) molecular species, including e-PC38:4 and e-PC38:5, those are presumed to bind polyunsaturated fatty acids. Chimyl alcohol treatment also increased ether-PC species, including e-PC36:4 and e-PC36:5 while monoacylglycerol did not alter ether-PC levels. These findings suggest that AKG plays a crucial role in membrane dynamics during myogenesis through metabolic conversion to ether-PLs, providing novel insights into muscle homeostasis to contribute to developing nutritional strategies and preventing and treating muscle diseases.
• Development of a Method for in vivo Direct Monitoring of Stored Fat Burning Using Stable Isotope-labeled Fatty Acids.
  Seiya Tanaka; Tetsujiro Matsui; Riho Murata; Aya Yoshinaga-Kiriake; Kazuaki Yoshinaga; Fumiaki Beppu; Hiroyuki Watanabe; Naohiro Gotoh
  Journal of oleo science, 74, 4, 423, 428, 2025, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, We developed a novel approach for direct and rapid evaluation of the fat-burning effects of various food ingredients in vivo. Male ICR mice were fed a diet containing stable isotope-labeled palmitic acid, and fat oxidation was quantified by measuring the production of labeled carbon dioxide (13CO2) generated during β-oxidation. By evaluating the 24-h fat burning profile, we demonstrated the immediate fat-burning effect of caffeine and the gradual fat-burning effect of curcumin. This methodology allows fat-burning-specific detection rather than fat absorption inhibition, thereby providing a powerful tool for comparing fat-burning effects of different food components in vivo.
• Identification of a novel monocyclic carotenoid and prediction of its biosynthetic genes in Algoriphagus sp. oki45.
  Naoki Takatani; Takashi Maoka; Tomoo Sawabe; Fumiaki Beppu; Masashi Hosokawa
  Applied microbiology and biotechnology, 108, 1, 102, 102, Dec. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Bacteria belonging to the genus Algoriphagus have been isolated from various sources, such as Antarctic sea ice, seawater, and sediment, and some strains are known to produce orange to red pigments. However, the pigment composition and biosynthetic genes have not been fully elucidated. A new red-pigmented Algoriphagus sp. strain, oki45, was isolated from the surface of seaweed collected from Senaga-Jima Island, Okinawa, Japan. Genome comparison revealed oki45's average nucleotide identity of less than 95% to its closely related species, Algoriphagus confluentis NBRC 111222 T and Algoriphagus taiwanensis JCM 19755 T. Comprehensive chemical analyses of oki45's pigments, including 1H and 13C nuclear magnetic resonance and circular dichroism spectroscopy, revealed that the pigments were mixtures of monocyclic carotenoids, (3S)-flexixanthin ((3S)-3,1'-dihydroxy-3',4'-didehydro-1',2'-dihydro-β,ψ-caroten-4-one) and (2R,3S)-2-hydroxyflexixanthin ((2R,3S)-2,3,1'-trihydroxy-3',4'-didehydro-1',2'-dihydro-β,ψ-caroten-4-one); in particular, the latter compound was new and not previously reported. Both monocyclic carotenoids were also found in A. confluentis NBRC 111222 T and A. taiwanensis JCM 19755 T. Further genome comparisons of carotenoid biosynthetic genes revealed the presence of eight genes (crtE, crtB, crtI, cruF, crtD, crtYcd, crtW, and crtZ) for flexixanthin biosynthesis. In addition, a crtG homolog gene encoding 2,2'-β-hydroxylase was found in the genome of the strains oki45, A. confluentis NBRC 111222 T, and A. taiwanensis JCM 19755 T, suggesting that the gene is involved in 2-hydroxyflexixanthin synthesis via 2-hydroxylation of flexixanthin. These findings expand our knowledge of monocyclic carotenoid biosynthesis in Algoriphagus bacteria. KEY POINTS: • Algoriphagus sp. strain oki45 was isolated from seaweed collected in Okinawa, Japan. • A novel monocyclic carotenoid 2-hydroxyflexixanthin was identified from strain oki45. • Nine genes for 2-hydroxyflexixanthin biosynthesis were found in strain oki45 genome.
• Identification of the Active EPA/AA-Binding Ether-Type Phosphatidylcholine Derived from the Starfish Patiria pectinifera for C2C12 Myotube Growth
  Aoi Fukushima; Kyosuke Imamura; Naoki Takatani; Masashi Hosokawa; Fumiaki Beppu
  ACS Omega, American Chemical Society (ACS), 30 Oct. 2024, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal
• Dietary 7,10-dihydroxy-8(E)-octadecenoic acid reduces fat accumulation and prevents hyperglycemia and hyperlipidemia in diabetic/obese KK-Ay mice
  Fumiaki Beppu; Shota Fuji; Hak-Ryul Kim; Ching T. Hou; Kazuo Miyashita; Masashi Hosokawa
  BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 61, Oct. 2024, [Peer-reviewed], [Lead author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal
• Apo-12'-capsorubinal exhibits anti-inflammatory effects and activates nuclear factor erythroid 2-related factor 2 in RAW264.7 macrophages.
  Naoki Takatani; Hiroki Miyafusa; Yumiko Yamano; Fumiaki Beppu; Masashi Hosokawa
  Archives of biochemistry and biophysics, 760, 110125, 110125, 16 Aug. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Apocarotenoids have short carbon chain structures cleaved at a polyene-conjugated double bond. They can be biosynthesized in plants and microorganisms. Animals ingest carotenoids through food and then metabolize them into apocarotenoids. Although several apocarotenoids have been identified in the body, their precise health functions are still poorly understood. This study investigated the anti-inflammatory activities of apo-12'-capsorubinal in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. It was confirmed that apo-12'-capsorubinal was not cytotoxic to the macrophages at the concentrations tested. Apo-12'-capsorubinal treatment led to a marked downregulation of interleukin (IL)-6 protein and Il6 mRNA levels. This apocarotenoid exhibited more potent inhibitory effects than its parent carotenoids, capsanthin and capsorubin. Furthermore, apo-12'-capsorubinal, but not its parent carotenoids, promoted the nuclear accumulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulated the expression of Nrf2-target genes, such as heme oxygenase 1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO-1), in a dose-dependent manner. Furthermore, a comparison using apo-12'-zeaxanthinal and 7,8-dihydro-8-oxo-apo-12'-zeaxanthinal revealed that the α, β-unsaturated carbonyl group on the polyene linear chain mediated the enhanced nuclear Nrf2 translocation, HO-1 expression, and inhibition of IL-6 production. In contrast, apo-12'-mytiloxanthinal, which harbored a hydroxyl group at C-8 of apo-12'-capsorubinal, did not exhibit any of these activities. These results indicated that the β carbon of the α, β-unsaturated carbonyl group in the linear part of the polyene chain is crucial to the Nrf2-activating and anti-inflammatory effects of apo-12'-capsorubinal. This study will advance our knowledge of the physiological significance of xanthophyll-derived apocarotenoids and their potential use as nutraceuticals and pharmaceuticals.
• Identification and tissue distribution of fucoxanthinol and amarouciaxanthin A fatty acid esters in fucoxanthin-fed mice.
  Naoki Takatani; Kana Sakimura; Kodai Nagata; Fumiaki Beppu; Yumiko Yamano; Takashi Maoka; Masashi Hosokawa
  Food chemistry, 410, 135318, 135318, 28 Dec. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Monocaprin Enhances Bioavailability of Fucoxanthin in Diabetic/Obese KK-Ay Mice
  Kodai Nagata; Naoki Takatani; Fumiaki Beppu; Aya Abe; Etsuko Tominaga; Tomohisa Fukuhara; Makoto Ozeki; Masashi Hosokawa
  Marine Drugs, 20, 7, 446, 446, MDPI AG, 07 Jul. 2022, [Peer-reviewed]
  Scientific journal, Fucoxanthin is a marine carotenoid found in brown seaweeds and several microalgae. It has been reported that fucoxanthin has health benefits such as anti-obesity and anti-diabetic effects. To facilitate fucoxanthin applications in the food industry, it is important to improve its low bioavailability. We attempted the combined feeding of fucoxanthin-containing seaweed oil (SO) and monocaprin in a powder diet and analyzed the fucoxanthin metabolite contents in the liver, small intestine and serum of diabetic/obese KK-Ay mice. After 4 weeks of feeding with the experimental diets, the serum fucoxanthinol concentrations of the mice fed 0.2% SO and 0.5% monocaprin were higher than those of the 0.2% SO-fed mice. Furthermore, fucoxanthinol accumulation in the liver and small intestine tended to increase in a combination diet of 0.2% SO and 0.125–0.5% monocaprin compared with a diet of 0.2% SO alone, although amarouciaxanthin A accumulation was not different among the 0.2% SO-fed groups. These results suggest that a combination of monocaprin with fucoxanthin-containing SO is an effective treatment for improving the bioavailability of fucoxanthin.
• Preparation of Apoastaxanthinals and Evaluation of Their Anti-inflammatory Action against Lipopolysaccharide-Stimulated Macrophages and Adipocytes
  Naoki Takatani; Fumiaki Beppu; Yumiko Yamano; Takashi Maoka; Kazuo Miyashita; Masashi Hosokawa
  ACS OMEGA, Jun. 2022, [Peer-reviewed]
  English, Scientific journal
• Comparison of Lipoprotein Cholesterol Levels in Golden Syrian Hamster Administrated trans-Octadecenoic Acid Positional Isomers.
  Koji Nagao; Junya Ueno; Kazuaki Yoshinaga; Toshiharu Nagai; Hoyo Mizobe; Akihiko Yoshida; Fumiaki Beppu; Seiya Tanaka; Naohiro Gotoh
  Journal of oleo science, 71, 4, 609, 618, 01 Apr. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, We previously conducted a study using HepG2 cells to compare the effect on the secreted apolipoprotein B-100 and apolipoprotein A-1 ratio (B-100/A-1) corresponding to the ratio of low-density to high-density lipoprotein cholesterol (LDL/HDL) among 13 types of trans-octadecenoic acid (t-18:1) positional isomers. The results revealed that trans-5-18:1 (t5) significantly increased B-100/A-1. In this study, 1% of t5 in the diet, corresponding to 2.08 energy%, was administrated golden Syrian hamsters for 4 weeks to reveal the effects on lipid profiles, including LDL/HDL, by comparing cis-9-octadecenoic acid (OA, oleic acid), trans-9-octadecenoic acid (EA), trans-11-octadecenoic acid (VA), and trans-9,trans-12- octadecadienoic acid (TT). LDL/HDL was not significantly different among the groups. However, the cholesterol concentration of medium very low-density lipoprotein (VLDL) was significantly lower in the TT diet than in the OA and t5 diets. The cholesterol concentration of small VLDL was significantly lower in the TT diet than in the OA, t5, and EA diets. The cholesterol concentration of large LDL was significantly lower in the TT diet than in the t5 and EA diets. However, no significant difference was detected between the TT and OA diets. In contrast, the cholesterol concentration of very small HDL was significantly higher in the TT diet than in the t5 diet. These results would support that lipid metabolism is affected by the structure of TFA in animals. However, t5-18:1 did not significantly change any lipid profile compared to OA existing in nature, and the previous result from the cell experiment showing that t5 increased B-100/A-1 (LDL/HDL) was not confirmed in this animal experiment.
• Reduction of serum lipid levels in diabetic obese KK-A ^y mice using monoalkyldiacylglycerol-rich oil derived from Berryteuthis magister
  Masaki Kakimoto; Naoki Takatani; Masashi Hosokawa; Fumiaki Beppu
  Fisheries Science, 89, 243, 251, 2022, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal
• Analysis of minor vitamin E homologues in natural products
  Fumiaki Beppu; Naohiro Gotoh
  Journal of Berry Research, 1, 12, IOS Press, 07 Sep. 2021, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal, Fruits and seeds are naturally rich in antioxidants, which provide protection against UV light and oxygen. Vitamin E is a typical antioxidant, and offers radical scavenging activity to prevent the oxidation of the polyunsaturated fatty acids in cell membranes, contribute to homeostasis, and support the biological functions of animals and plants. Tocopherol and tocotrienol are forms of vitamin E widely found in natural products. In addition, recent studies have demonstrated the existence of minor homologues, namely tocomonoenol and tocodienol. The characteristic physiological functions of vitamin E homologues are dependent on their structure. Consequently, the nutritional functionality of the minor homologues has attracted much research interest, which relies on highly accurate analytical methods. This review aimed to summarize the literature concerning the diversity of tocomonoenol and tocodienol, as well as reports on the various methods for their analysis and detection in different sample matrices. This paper is expected to contribute to future exploratory research and the functional evaluation of minor homologues., 13849844;13849843
• n-3 Polyunsaturated fatty acid-enriched phosphatidylglycerol suppresses inflammation in RAW264.7 cells through Nrf2 activation via alteration of fatty acids in cellular phospholipids
  Liping Chen; Fumiaki Beppu; Naoki Takatani; Kazuo Miyashita; Masashi Hosokawa
  FISHERIES SCIENCE, 87, 5, 727, 737, Sep. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Simultaneous Treatment of Long-chain Monounsaturated Fatty Acid and n-3 Polyunsaturated Fatty Acid Decreases Lipid and Cholesterol Levels in HepG2 Cell.
  Kazuaki Yoshinaga; Yota Mizuno; Samanthika Senarath; Aya Yoshinaga-Kiriake; Toshiharu Nagai; Fumiaki Beppu; Seiya Tanaka; Naohiro Gotoh
  Journal of oleo science, 70, 5, 731, 736, 01 May 2021, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The n-3 type polyunsaturated fatty acids (n-3PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), from fish oil exhibit health benefits such as triacylglycerol- and cholesterol-lowering effects. Some pelagic fishes contain long-chain monounsaturated fatty acids (LC-MUFAs) such as eicosenoic acid (C20:1), which exert health-promoting effects. However, no study has evaluated beneficial effects of n-3PUFA and LC-MUFA combination. Here, we investigated effects of simultaneous treatment with n-3PUFA (EPA and DHA) and LC-MUFA (cis-5-C20:1 and cis-7-C20:1) and found that n-3PUFA and LC-MUFA combination significantly decreased lipid accumulation and reduced total cholesterol in HepG2 cells. Cholesterol level was significantly lower in DHA + cis-7-C20:1 group than in DHA + EPA group. These results suggest the importance of LC-MUFA as a functional molecule in fish oil.
• Seco-type β-Apocarotenoid Generated by β-Carotene Oxidation Exerts Anti-inflammatory Effects against Activated Macrophages.
  Naoki Takatani; Fumiaki Beppu; Yumiko Yamano; Takashi Maoka; Masashi Hosokawa
  Journal of oleo science, 70, 4, 549, 558, 02 Apr. 2021, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, β-Apocarotenoids are the cleavage products of β-carotene. They are found in plants, carotenoid-containing foods, and animal tissues. However, limited information is available regarding the health benefits of β-apocarotenoids. Here, we prepared seco-type β-apocarotenoids through the chemical oxidation of β-carotene and investigated their anti-inflammatory effects against activated macrophages. Oxidation of β-carotene with potassium permanganate produced seco-β-apo-8'-carotenal, in which one end-group formed an "open" β-ring and the other was cleaved at the C-7',8' position. In lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells, seco-β-apo-8'-carotenal inhibited the secretion and mRNA expression of inflammatory mediators such as nitric oxide, interleukin (IL)-6 and IL-1β, and monocyte chemoattractant protein-1. Furthermore, seco-β-apo-8'-carotenal suppressed phosphorylation of c-Jun N-terminal kinase and the inhibitor of nuclear factor (NF)-κB as well as the nuclear accumulation of NF-κB p65. Notably, since seco-β-apo-8'-carotenal exhibited remarkable anti-inflammatory activity compared with β-apo-8'-carotenal, its anti-inflammatory action could depend on the opened β-ring structure. These results suggest that seco-β-apo-8'-carotenal has high potential for the prevention of inflammation-related diseases.
• Incorporation of Dietary Arachidonic and Docosatetraenoic Acid into Mouse Brain.
  Kazuaki Yoshinaga; Haruna Ishikawa; Fumiaki Beppu; Naohiro Gotoh
  Journal of agricultural and food chemistry, 69, 8, 2457, 2461, 03 Mar. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, It is essential to analyze the metabolism of dietary polyunsaturated fatty acids in the brain for the research and development of functional foods. In this study, a single dose of 2,2-dideuterium-labeled docosatetraenoic acid ((+2)DTA) or 2,2-dideuterium-labeled arachidonic acid ((+2)AA) was orally administered to Institute of Cancer Research (ICR) mice and its metabolism in the brain was investigated. In the (+2)DTA group, the (+2)DTA content in the brain was significantly increased at 4, 8, 24, and 96 h compared to 0 h after administration, while in the (+2)AA group, the (+2)AA content was significantly increased at 4, 8, 24, and 96 h compared to 0 h. However, there was no significant difference in the content of (+2)DTA, a metabolite of (+2)AA, among all the groups. These results suggest that dietary (+2)DTA and (+2)AA pass through the blood-brain barrier and dietary (+2)AA is rather stored in the brain than converted to (+2)DTA.
• Identification of Paracentrone in Fucoxanthin-Fed Mice and Anti-Inflammatory Effect against Lipopolysaccharide-Stimulated Macrophages and Adipocytes.
  Naoki Takatani; Daisuke Taya; Ami Katsuki; Fumiaki Beppu; Yumiko Yamano; Akimori Wada; Kazuo Miyashita; Masashi Hosokawa
  Molecular nutrition & food research, 65, 2, e2000405, Jan. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, SCOPE: Fucoxanthin is converted to fucoxanthinol and amarouciaxanthin A in the mouse body. However, further metabolism such as cleavage products (i.e., apocarotenoids) remains unclear. The fucoxanthin-derived apocarotenoid in vivo is investigated and the anti-inflammatory effect of apocarotenoids with fucoxanthin partial structure such as allenic bond and epoxide residue against activated macrophages and adipocytes in vitro is evaluated. METHODS AND RESULTS: LC-MS analysis indicates the presence of paracentrone, a C31 -allenic-apocarotenoid, in white adipose tissue of diabetic/obese KK-Ay and normal C57BL/6J mice fed 0.2% fucoxanthin diet for 1 week. In lipopolysaccharide-activated RAW264.7 macrophages, paracentrone as well as C26 - and C28 -allenic-apocarotenoids suppresses the overexpression of inflammatory factors. Further, apo-10'-fucoxanthinal, a fucoxanthin-derived apocarotenoid which retained epoxide residue, exhibits a most potent anti-inflammatory activity through regulating mitogen-activated protein kinases and nuclear factor-κB inflammatory signal pathways. In contrast, β-apo-8'-carotenal without allenic bond and epoxide residue lacks suppressed inflammation. In 3T3-L1 adipocytes, paracentrone, and apo-10'-fucoxanthinal downregulate the mRNA expression of proinflammatory mediators and chemokines induced by co-culture with RAW264.7 cells. CONCLUSION: Dietary fucoxanthin accumulates as paracentrone as well as fucoxanthinol and amarouciaxanthin A in the mouse body. Allenic bond and epoxide residue of fucoxanthin-derived apocarotenoids have pivotal roles for anti-inflammatory action against activated macrophages and adipocytes.
• Application of Partial Hydrogenation for the Generation of Minor Tocochromanol Homologs and Functional Evaluation of Hydrogenated Tocotrienol-rich Vitamin E Oil in Diabetic Obese Mice.
  Fumiaki Beppu; Aimi Sakuma; Satoshi Kasatani; Yoshinori Aoki; Naohiro Gotoh
  Journal of oleo science, 70, 1, 103, 112, 2021, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
  English, Scientific journal, Recent research has identified minor homologs of vitamin E with one or two double bonds in the side-chain, namely tocomonoenol (T1) and tocodienol (T2), in natural products. We first explored the effectiveness of partial hydrogenation for generating minor tocochromanols from tocotrienol (T3). During hydrogenation with pure α-T3 as a substrate, the side-chain was partially saturated in a time-dependent manner, and a large amount of α-T1 and α-T2 was obtained. To investigate the beneficial effects of the hydrogenated product, we fed diabetic obese KK-A y mice with a hydrogenated T3 mixture (HT3). Feeding HT3 revealed tissue-specific accumulation of tocochromanols, ameliorated hyperglycemia and improved ratio of high-density lipoprotein cholesterol to total cholesterol in serum, with invariant body weight and fat mass. Hence, we propose that hydrogenation is a useful method for generating T1 and T2 homologs, which can be applied to explore the structure-related function of tocochromanols.
• Fucoxanthin inhibits hepatic oxidative stress, inflammation, and fibrosis in diet-induced nonalcoholic steatohepatitis model mice.
  Naoki Takatani; Yuka Kono; Fumiaki Beppu; Yuko Okamatsu-Ogura; Yumiko Yamano; Kazuo Miyashita; Masashi Hosokawa
  Biochemical and biophysical research communications, 528, 2, 305, 310, 23 Jul. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Nonalcoholic steatohepatitis (NASH) is associated with hepatocyte injury, excessive oxidative stress, and chronic inflammation in fatty liver, and can progress to more severe liver diseases, such as cirrhosis and hepatocellular carcinoma. However, currently there are no effective therapies for NASH. Marine carotenoid, fucoxanthin (Fx), abundant in brown seaweeds, has variable biological properties, such as anti-cancer, anti-inflammatory, anti-oxidative and anti-obesity. However, the effect of Fx on the development of NASH has not been explored. We investigated the protective effects of Fx in diet-induced NASH model mice fed choline-deficient L-amino acid-defined high fat diet (CDAHFD). Fx administration significantly attenuated liver weight gain and hepatic fat accumulation, resulting in the alleviation of hepatic injury. Furthermore, the Fx-fed mice, not only exhibited reduced hepatic lipid oxidation, but also decreased mRNA expression levels of inflammation and infiltration-related genes compared to that of the CDAHFD-fed mice. Moreover, fucoxanthinol and amarouciaxanthin A, two Fx metabolites exerted anti-inflammatory effects in the liver via inhibiting the chemokine production in hepatocytes. In case of fibrosis, one of the features of advanced NASH, the expression of fibrogenic factors including activated-hepatic stellate cell marker was significantly decreased in the liver of Fx-fed mice. Thus, the present study elucidated that dietary Fx not only inhibited hepatic oxidative stress and inflammation but also prevented early phase of fibrosis in the diet-induced NASH model mice.
• Bioactive significance of fucoxanthin and its effective extraction
  Kazuo Miyashita; Fumiaki Beppu; Masashi Hosokawa; Xiaoyong Liu; Shuzhou Wang
  BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 26, Jul. 2020, [Peer-reviewed]
  English, Scientific journal
• Nutraceutical characteristics of the brown seaweed carotenoid fucoxanthin.
  Kazuo Miyashita; Fumiaki Beppu; Masashi Hosokawa; Xiaoyong Liu; Shuzhou Wang
  Archives of biochemistry and biophysics, 686, 108364, 108364, 15 Jun. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Fucoxanthin (Fx), a major carotenoid found in brown seaweed, is known to show a unique and wide variety of biological activities. Upon absorption, Fx is metabolized to fucoxanthinol and amarouciaxanthin, and these metabolites mainly accumulate in visceral white adipose tissue (WAT). As seen in other carotenoids, Fx can quench singlet oxygen and scavenge a wide range of free radicals. The antioxidant activity is related to the neuroprotective, photoprotective, and hepatoprotective effects of Fx. Fx is also reported to show anti-cancer activity through the regulation of several biomolecules and signaling pathways that are involved in either cell cycle arrest, apoptosis, or metastasis suppression. Among the biological activities of Fx, anti-obesity is the most well-studied and most promising effect. This effect is primarily based on the upregulation of thermogenesis by uncoupling protein 1 expression and the increase in the metabolic rate induced by mitochondrial activation. In addition, Fx shows anti-diabetic effects by improving insulin resistance and promoting glucose utilization in skeletal muscle.
• Effective extraction of carotenoids from brown seaweeds and vegetable leaves with edible oils
  Kazuyoshi Teramukai; Shusuke Kakui; Fumiaki Beppu; Masashi Hosokawa; Kazuo Miyashita
  INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, 60, Mar. 2020, [Peer-reviewed]
  English, Scientific journal
• Functional evaluation of marine-derived tocopherol, a minor homolog of vitamin E, on adipocyte differentiation and inflammation using 3T3-L1 and RAW264.7 cells
  Fumiaki Beppu; Yusuke Aida; Masahiro Kaneko; Satoshi Kasatani; Yoshinori Aoki; Naohiro Gotoh
  Fisheries Science, 86, 2, 415, 425, Springer Science and Business Media LLC, Mar. 2020, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  Scientific journal
• The Effect of n-3 PUFA Binding Phosphatidylglycerol on Metabolic Syndrome-Related Parameters and n-3 PUFA Accretion in Diabetic/Obese KK-Ay Mice.
  Liping Chen; Naoki Takatani; Fumiaki Beppu; Kazuo Miyashita; Masashi Hosokawa
  Nutrients, 11, 12, 22 Nov. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, n-3 Polyunsaturated fatty acid binding phospholipids (n-3 PUFA-PLs) are known to be potent carriers of n-3 PUFAs and provide health benefits. We previously prepared n-3 PUFA binding phosphatidylglycerol (n-3 PUFA-PG) by phospholipase D-mediated transphosphatidylation. Because PG has excellent emulsifiability, n-3 PUFA-PG is expected to work as a functional molecule with properties of both PG and n-3 PUFAs. In the present study, the health benefits and tissue accretion of dietary n-3 PUFA-PG were examined in diabetic/obese KK-Ay mice. After a feeding duration over 30 days, n-3 PUFA-PG significantly reduced the total and non-HDL cholesterols in the serum of diabetic/obese KK-Ay mice. In the mice fed n-3 PUFA-PG, but not n-3 PUFA-TAG, hepatic lipid content was markedly alleviated depending on the neutral lipid reduction compared with the SoyPC-fed mice. Further, the n-3 PUFA-PG diet increased eicosapentaenoic acid and docosahexaenoic acid (DHA) and reduced arachidonic acid in the small intestine, liver, perirenal white adipose tissue, and brain, and the ratio of the n-6 PUFAs to n-3 PUFAs in those tissues became lower compared to the SoyPC-fed mice. Especially, the DHA level was more significantly elevated in the brains of n-3 PUFA-PG-fed mice compared to the SoyPC-fed mice, whereas n-3 PUFA-TAG did not significantly alter DHA in the brain. The present results indicate that n-3 PUFA-PG is a functional lipid for reducing serum and liver lipids and is able to supply n-3 PUFAs to KK-Ay mice.
• Preparation of n-3 Polyunsaturated Phosphatidylglycerol from Salmon Roe Lipids by Phospholipase D and In Vitro Digestion
  Chen Liping; Beppu Fumiaki; Miyashita Kazuo; Hosokawa Masashi
  EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 122, 2, 21 Oct. 2019, [Peer-reviewed]
  English, Scientific journal
• Examination of the Catabolic Rates of 13C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol Using 13CO2 Expired from Mice.
  Kazuaki Yoshinaga; Fumiaki Beppu; Yoshio Yamatani; Atsushi Kubo; Aya Yoshinaga-Kiriake; Toshiharu Nagai; Akihiko Yoshida; Jota Kanda; Naohiro Gotoh
  Journal of oleo science, 68, 6, 591, 598, 06 Jun. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Comparison of the Effects of Long-chain Monounsaturated Fatty Acid Positional Isomers on Lipid Metabolism in 3T3-L1 Cells.
  Samanthika Senarath; Fumiaki Beppu; Kazuaki Yoshinaga; Toshiharu Nagai; Akihiko Yoshida; Naohiro Gotoh
  Journal of oleo science, 68, 4, 379, 387, 01 Apr. 2019, [Peer-reviewed], [Domestic magazines]
  English
• Evaluating the Content and Distribution of Trans Fatty Acid Isomers in Foods Consumed in Japan.
  Naohiro Gotoh; Kazuaki Yoshinaga; Satoshi Kagiono; Yukiko Katoh; Yota Mizuno; Fumiaki Beppu; Toshiharu Nagai; Hoyo Mizobe; Akihiko Yoshida; Koji Nagao
  Journal of oleo science, 68, 2, 193, 202, 01 Feb. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Effect of calcium treatment on catabolic rates of 13C-labeled fatty acids bound to the α and β positions of triacylglycerol.
  K. Yoshinaga; F. Beppu; Y Yamatani; A. Kubo; A. Yoshinaga-Kiriake; T. Nagai; A. Yoshida; J. Kanda; N. Gotoh
  J. Oleo Sci., in press, 11, 1149, 1155, 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The absorption efficacies and catabolic rates of fatty acids are affected by their binding position on triacylglycerol (TAG). However, the kind of effect calcium treatment has on the catabolism of fatty acids is unclear. In this study, the catabolic rates of 13C-labeled palmitic acid, oleic acid, and linoleic acid bound to sn-1, 3 (α) and sn-2 (β) position of TAG in the presence of calcium were compared using isotope ratio mass spectrometry. The catabolic rates of 13C-labeled fatty acids were evaluated using the ratio of 13C to 12C in the carbon dioxide expired by mice. The catabolic rate of palmitic acid bound to the α position was significantly lower than that of palmitic acid bound to the β position of TAG. The rates of 13CO2 formation from palmitic acid at the β position remained higher for a long time. In contrast, oleic and linoleic acids at the α position were as well catabolized as those at the β position. These results indicate that in the presence of calcium, the saturated fatty acid bound to the β position is highly catabolized, whereas that bound to the α position is not well catabolized. Saturated fatty acid at the α position is hydrolyzed by pancreatic lipase to promptly form insoluble complexes with calcium, which are excreted from the body, and thereby reducing the catabolic rate of these fatty acids.
• Metabolism of Natural Highly Unsaturated Fatty Acid, Tetracosahexaenoic Acid (24:6n-3), in C57BL/KsJ-db/db Mice.
  Naohiro Gotoh; Koji Nagao; Hiroki Ishida; Kazusa Nakamitsu; Kazuaki Yoshinaga; Toshiharu Nagai; Fumiaki Beppu; Aya Yoshinaga-Kiriake; Hiroyuki Watanabe; Teruyoshi Yanagita
  Journal of oleo science, 67, 12, 1597, 1607, 01 Dec. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Simple Quantification of Lactones in Milk Fat by Solvent Extraction Using Gas Chromatography-Mass Spectrometry.
  Junji Obi; Kazuaki Yoshinaga; Arisa Tago; Toshiharu Nagai; Akihiko Yoshida; Fumiaki Beppu; Naohiro Gotoh
  Journal of oleo science, 67, 8, 941, 948, 01 Aug. 2018, [Peer-reviewed], [Domestic magazines]
  English
• Differential Effect of cis-Eicosenoic Acid Positional Isomers on Adipogenesis and Lipid Accumulation in 3T3-L1 Cells
  Samanthika Senarath; Kazuaki Yoshinaga; Toshiharu Nagai; Akihiko Yoshida; Fumiaki Beppu; Naohiro Gotoh
  European Journal of Lipid Science and Technology, 120, 6, Jun. 2018, [Peer-reviewed]
  Scientific journal
• Study of Trans Fatty Acid Formation in Oil by Heating Using Model Compounds.
  Naohiro Gotoh; Satoshi Kagiono; Kazuaki Yoshinaga; Hoyo Mizobe; Toshiharu Nagai; Akihiko Yoshida; Fumiaki Beppu; Koji Nagao
  Journal of oleo science, 67, 3, 273, 281, 01 Mar. 2018, [Peer-reviewed], [Domestic magazines]
  English
• Quantitative analysis of the accumulation of marine-derived tocopherol in the tissue of mice fed with salmon roe oil using HPLC-fluorescence
  Fumiaki Beppu; Hitomi Kondo; Satoshi Kasatani; Yoshinori Aoki; Naohiro Gotoh
  Journal of Oleo Science, 67, 3, 283, 288, 2018, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Homochiral Asymmetric Triacylglycerol Isomers in Egg Yolk
  Toshiharu Nagar; Keiko Ishikawa; Kazuaki Yoshinagal; Akihiko Yoshida; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 66, 12, 1293, 1299, Dec. 2017, [Peer-reviewed]
  English, Scientific journal
• Quantification of Fraglide-1, a New Functional Ingredient, in Vinegars
  Aydan H. Yatmazt; Tetsuaki Kinoshita; Akio Miyazato; Masahiro Takagi; Yoshio Tsujino; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 66, 12, 1381, 1386, Dec. 2017, [Peer-reviewed]
  English, Scientific journal
• Comparison of the Distribution of Unsaturated Fatty Acids at the Sn-2 Position of Phospholipids and Triacylglycerols in Marine Fishes and Mammals
  Fumiaki Beppu; Keiko Yasuda; Ayako Okada; Yoshitsugu Hirosaki; Masako Okazaki; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 66, 11, 1217, 1227, Nov. 2017, [Peer-reviewed]
  English, Scientific journal
• Comparison of the Effect of trans Fatty Acid Isomers on Apolipoprotein A1 and B Secretion in HepG2 Cells
  Koji Nagao; Kazuaki Yoshinaga; Toshiharu Nagai; Hoyo Mizobe; Akihiko Yoshida; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 66, 10, 1175, 1181, Oct. 2017, [Peer-reviewed]
  English, Scientific journal
• Dietary Starfish Oil Prevents Hepatic Steatosis and Hyperlipidemia in C57BLI6N Mice Fed High-fat Diet
  Fumiaki Beppu; Haoqi Li; Kazuaki Yoshinaga; Toshiharu Nagai; Akihiko Yoshinda; Atsushi Kubo; Jota Kanda; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 66, 7, 761, 769, Jul. 2017, [Peer-reviewed]
  English, Scientific journal
• Acute Effect of Metformin on Postprandial Hypertriglyceridemia through Delayed Gastric Emptying
  Daisuke Sato; Katsutaro Morino; Fumiyuki Nakagawa; Koichiro Murata; Osamu Sekine; Fumiaki Beppu; Naohiro Gotoh; Satoshi Ugi; Hiroshi Maegawa
  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18, 6, Jun. 2017, [Peer-reviewed]
  English, Scientific journal
• Quantification of Triacylglycerol Molecular Species in Edible Fats and Oils by Gas Chromatography-Flame Ionization Detector Using Correction Factors
  Kazuaki Yoshinaga; Junji Obi; Toshiharu Nagai; Hiroyuki Iioka; Akihiko Yoshida; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 66, 3, 259, 268, Mar. 2017, [Peer-reviewed]
  English, Scientific journal
• Quantitative Analysis of the Distribution of cis-Eicosenoic Acid Positional Isomers in Marine Fishes from the Indian Ocean
  Samanthika Senarath; Kazuaki Yoshinaga; Toshiharu Nagai; Akihiko Yoshida; Fumiaki Beppu; Chamila Jayasinghe; Chandravathany Devadawson; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 66, 2, 187, 197, Feb. 2017, [Peer-reviewed]
  English, Scientific journal
• Comparison of 13C-labeled fatty acid catabolic rates bound to the sn-1, sn-2, and sn-3 positions of triacylglycerol using 13C-labeled CO2 expired from mice.
  F. Beppu; T. Kawamatsu; Y. Yamatani; T. Nagai; K. Yoshinaga; H. Mizobe; A. Yoshida; A. Kubo; J. Kanda; N. Gotoh
  J. Oleo Sci., 66, 85, 91, 2017, [Peer-reviewed]
  English, Scientific journal
• Comparison of Catabolic Rates of sn-1, sn-2, and sn-3 Fatty Acids in Triacylglycerols Using (CO2)-C-13 Breath Test in Mice
  Fumiaki Beppu; Takashi Kawamatsu; Yoshio Yamatani; Toshiharu Nagai; Kazuaki Yoshinaga; Hoyo Mizobe; Akihiko Yoshida; Atsushi Kubo; Jota Kanda; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 66, 1, 85, 91, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• Quantification of Triacylglycerol Positional Isomers in Rat Milk
  Natsuko Watanabe; Toshiharu Nagai; Hoyo Mizobe; Kazuaki Yoshinaga; Akihiko Yoshida; Yohei Kitamura; Takashi Shimizu; Fumiaki Beppu; Naohiro Gotohl
  JOURNAL OF OLEO SCIENCE, 65, 12, 977, 983, Dec. 2016, [Peer-reviewed]
  English, Scientific journal
• Comparative Analysis of Lipid Content and Fatty Acid Composition of Commercially Important Fish and Shellfish from Sri Lanka and Japan
  Chandravathany Devadason; Chamila Jayasinghe; Ramiah Sivakanesan; Samanthika Senarath; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 65, 7, 543, 556, Jul. 2016, [Peer-reviewed]
  English, Scientific journal
• Trans-octadecenoic Acid Positional Isomers Have Different Accumulation and Catabolism Properties in Mice
  Fumiaki Beppu; Masaharu Asanuma; Yoshinori Kawamura; Toshiharu Nagai; Kazuaki Yoshinaga; Hoyo Mizobe; Koichi Kojima; Satoshi Kasatani; Koji Nagao; Atsushi Kubo; Jota Kanda; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 64, 11, 1159, 1167, Nov. 2015, [Peer-reviewed]
  English, Scientific journal
• Abundances of Triacylglycerol Positional Isomers and Enantiomers Comprised of a Dipalmitoylglycerol Backbone and Short- or Medium-chain Fatty Acids in Bovine Milk Fat
  Toshiharu Nagai; Natsuko Watanabe; Kazuaki Yoshinaga; Hoyo Mizobe; Koichi Kojima; Lkuma Kuroda; Yuki Odanaka; Tadao Saito; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 64, 9, 943, 952, Sep. 2015, [Peer-reviewed]
  English, Scientific journal
• Generation of Tetracosahexaenoic Acid in Benthic Marine Organisms
  Rei Suo; Haoqi Li; Kazuaki Yoshinaga; Toshiharu Nagai; Hoyo Mizobe; Koichi Kojima; Koji Nagao; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 64, 7, 721, 727, Jul. 2015, [Peer-reviewed]
  English, Scientific journal
• Regiospecific Distribution of trans-Octadecenoic Acid Positional Isomers in Triacylglycerols of Partially Hydrogenated Vegetable Oil and Ruminant Fat
  Kazuaki Yoshinaga; Yoshinori Kawamura; Takashi Kitayama; Toshiharu Nagai; Hoyo Mizobe; Koichi Kojima; Yomi Watanabe; Shinichi Sato; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 64, 6, 617, 624, Jun. 2015, [Peer-reviewed]
  English, Scientific journal
• Comparison of catabolic rates of C-13-labeled palmitic acid bound to the alpha and beta positions of triacylglycerol using CO2 expired from mice
  Fumiaki Beppu; Kaoru Konno; Takashi Kawamatsu; Toshiharu Nagai; Kazuaki Yoshinaga; Hoyo Mizobe; Koichi Kojima; Hiroyuki Watanabe; Naohiro Gotoh
  EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 117, 5, 718, 723, May 2015, [Peer-reviewed]
  English, Scientific journal
• Effects of Imidacloprid on Myogenesis in C2C12 Myoblasts
  Amanda Rutherford; Yoo Kim; Fumiaki Beppu; Yeonhwa Park
  FASEB JOURNAL, 29, Apr. 2015, [Peer-reviewed]
  English
• Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice (vol 26, pg 57, 2015)
  Kazuaki Yoshinaga; Keiichi Sasaki; Hiroyuki Watanabe; Koji Nagao; Nao Inoue; Bungo Shirouchi; Teruyoshi Yanagita; Toshiharu Nagai; Hoyo Mizobe; Koichi Kojima; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 26, 4, 431, 432, Apr. 2015, [Peer-reviewed]
  English
• Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice
  Kazuaki Yoshinaga; Keiichi Sasaki; Hiroyuki Watanabe; Koji Nagao; Nao Inoue; Bungo Shirouchi; Teruyoshi Yanagita; Toshiharu Nagai; Hoyo Mizobe; Koichi Kojima; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 26, 1, 57, 63, Jan. 2015, [Peer-reviewed]
  English, Scientific journal
• Characterization of cis- and trans-octadecenoic acid positional isomers in edible fat and oil using gas chromatography-flame ionisation detector equipped with highly polar ionic liquid capillary column
  Kazuaki Yoshinaga; Masaharu Asanuma; Hoyo Mizobe; Koichi Kojima; Toshiharu Nagai; Fumiaki Beppu; Naohiro Gotoh
  FOOD CHEMISTRY, 160, 39, 45, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells
  Koji Nagao; Kazusa Nakamitsu; Hiroki Ishida; Kazuaki Yoshinaga; Toshiharu Nagai; Hoyo Mizobe; Koichi Kojima; Teruyoshi Yanagita; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 63, 10, 979, 985, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• Resolution Behavior of cis- and trans-Octadecenoic Acid Isomers by AOCS Official Method Using SP-2560 Column
  Kazuaki Yoshinaga; Masaharu Asanuma; Chao Xu; Hoyo Mizobe; Koichi Kojima; Toshiharu Nagai; Fumiaki Beppu; Naohiro Gotoh
  JOURNAL OF OLEO SCIENCE, 62, 10, 781, 788, Oct. 2013, [Peer-reviewed]
  English, Scientific journal
• Down-Regulation of Hepatic Stearoyl-CoA Desaturase-1 Expression by Fucoxanthin via Leptin Signaling in Diabetic/Obese KK-A (y) Mice
  Fumiaki Beppu; Masashi Hosokawa; Mi-Jin Yim; Taro Shinoda; Kazuo Miyashita
  LIPIDS, 48, 5, 449, 455, May 2013, [Peer-reviewed]
  English, Scientific journal
• Quantification of triacylglycerol molecular species in cocoa butter using high-performance liquid chromatography equipped with nano quantity analyte detector.
  Fumiaki Beppu; Toshiharu Nagai; Kazuaki Yoshinaga; Hoyo Mizobe; Koichi Kojima; Naohiro Gotoh
  Journal of oleo science, 62, 10, 789, 94, 2013, [Peer-reviewed], [Domestic magazines]
  English
• Actual ratios of triacylglycerol positional isomers and enantiomers comprising saturated fatty acids and highly unsaturated fatty acids in fishes and marine mammals.
  Toshiharu Nagai; Yumiko Matsumoto; Yanying Jiang; Keiko Ishikawa; Tokuhisa Wakatabe; Hoyo Mizobe; Kazuaki Yoshinaga; Koichi Kojima; Ikuma Kuroda; Tadao Saito; Fumiaki Beppu; Naohiro Gotoh
  Journal of oleo science, 62, 12, 1009, 15, 2013, [Peer-reviewed], [Domestic magazines]
  English
• Effects of dietary fucoxanthin on cholesterol metabolism in diabetic/obese KK-A(y) mice.
  Fumiaki Beppu; Masashi Hosokawa; Yoshimi Niwano; Kazuo Miyashita
  Lipids in health and disease, 11, 112, 112, 10 Sep. 2012, [Peer-reviewed], [International Magazine]
  English
• In vivo antioxidant activity of fucoxanthin on obese/diabetes KK-Ay mice.
  S. Iwasaki; M. A-K. Widjaja-Adhi; A. Koide; T. Kaga; S. Nakano; F. Beppu; M. Hosokawa; K. Miyashita
  Food Nutr. Sci., 3, 1491, 1499, 2012, [Peer-reviewed]
  English
• In Vivo and in Vitro Toxicity Studies of Fucoxanthin, a Marine Carotenoid
  Yoshimi Niwano; Fumiaki Beppu
  Handbook of Marine Macroalgae: Biotechnology and Applied Phycology, 319, 328, John Wiley and Sons, 21 Nov. 2011, [Peer-reviewed]
  English, In book
• The allenic carotenoid fucoxanthin, a novel marine nutraceutical from brown seaweeds
  Kazuo Miyashita; Sho Nishikawa; Fumiaki Beppu; Takayuki Tsukui; Masayuki Abe; Andmasashi Hosokawa
  JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 91, 7, 1166, 1174, May 2011, [Peer-reviewed]
  English
• 褐藻由来フコキサンチンによるstearoyl-CoA desaturase 1(SCD1)発現抑制効果
  篠田 太郎; 別府 史章; 津久井 隆行; 細川 雅史; 宮下 和夫
  日本栄養・食糧学会大会講演要旨集, 65回, 163, 163, (公社)日本栄養・食糧学会, Apr. 2011
  Japanese
• Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-A(y) mice
  Masashi Hosokawa; Tatsuya Miyashita; Sho Nishikawa; Shingo Emi; TakaYuki Tsukui; Fumiaki Beppu; Tomoko Okada; Kazuo Miyashita
  ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 504, 1, 17, 25, Dec. 2010, [Peer-reviewed]
  English, Scientific journal
• In vitro and in vivo evaluation of mutagenicity of fucoxanthin (FX) and its metabolite fucoxanthinol (FXOH)
  Fumiaki Beppu; Yoshimi Niwano; Emiko Sato; Masahiro Kohno; Takayuki Tsukui; Masashi Hosokawa; Kazuo Miyashita
  JOURNAL OF TOXICOLOGICAL SCIENCES, 34, 6, 693, 698, Dec. 2009, [Peer-reviewed]
  English
• Single and repeated oral dose toxicity study of fucoxanthin (FX), a marine carotenoid, in mice
  Fumiaki Beppu; Yoshimi Niwano; Takayuki Tsukui; Masashi Hosokawa; Kazuo Miyashita
  JOURNAL OF TOXICOLOGICAL SCIENCES, 34, 5, 501, 510, Oct. 2009, [Peer-reviewed]
  English, Scientific journal
• Hypolipidemic Effects of Ethanol Extracts of Citrus depressa and Annona atemoya, Typical Plant Foodstuffs in Okinawa, Japan on KKAy Mice Fed with Moderately High Fat Diet
  Fumiaki Beppu; Yoshimi Niwano; Rika Kyan; Kazumasa Yasura; Minori Tamaki; Michinori Nishino; Yoshiyuki Midorikawa; Hiroki Hamada
  FOOD SCIENCE AND TECHNOLOGY RESEARCH, 15, 5, 553, 556, Sep. 2009, [Peer-reviewed]
  English, Scientific journal
• Extensive Screening for Plant Foodstuffs in Okinawa, Japan with Anti-Obese Activity on Adipocytes In Vitro
  Yoshimi Niwano; Fumiaki Beppu; Taichi Shimada; Rika Kyan; Kazumasa Yasura; Minori Tamaki; Michinori Nishino; Yoshiyuki Midorikawa; Hiroki Hamada
  PLANT FOODS FOR HUMAN NUTRITION, 64, 1, 6, 10, Mar. 2009, [Peer-reviewed]
  English, Scientific journal
• ナノマイザー技術を応用した全粒大豆を用いた大豆飲料の開発
  島田太一; 別府史章; 庭野吉己; 北川昭夫; 上東亨子; 佐藤恵美子; 河野雅弘
  月刊 FOOD STYLE21 食品の機能と健康を考える化学情報誌, 142, 3, 86, 89, 食品化学新聞社, 2009
  Japanese, Research society
• フコキサンチンの安全性評価
  別府 史章; 倉智 麻木; 庭野 吉己; 津久井 隆行; 前多 隼人; 細川 雅史; 宮下 和夫
  日本栄養・食糧学会大会講演要旨集, 62回, 278, 278, (公社)日本栄養・食糧学会, Apr. 2008, [Peer-reviewed]
  Japanese
• 9trans,11trans conjugated linoleic acid inhibits the development of azoxymethane-induced colonic aberrant crypt foci in rats
  Yasui, Yumiko; Suzuki, Rikako; Kohno, Hiroyuki; Miyamoto, Shingo; Beppu, Fumiaki; Hosokawa, Masashi; Miyashita, Kazuo; Tanaka, Takuji
  NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 59, 1, 82, 91, 2007, [Peer-reviewed]
  English, Scientific journal
• Potent inhibitory effect of trans9, trans 11 isomer of conjugated linoleic acid on the growth of human colon cancer cells
  Fumiaki Beppu; Masashi Hosokawa; Leo Tanaka; Hiroyuki Kohno; Takuji Tanaka; Kazuo Miyashita
  JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 17, 12, 830, 836, Dec. 2006, [Peer-reviewed]
  English, Scientific journal

• Quantification of Triacylglycerol Molecular Species in Edible Fats and Oils by Gas Chromatography-Flame Ionization Detector Using Correction Factors (vol 66, pg 259, 2017)
  Kazuaki Yoshinaga; Junji Obi; Toshiharu Nagai; Hiroyuki Iioka; Akihiko Yoshida; Fumiaki Beppu; Naohiro Gotoh, JOURNAL OF OLEO SCIENCE, 74, 4, 435, 435, Apr. 2025
  English, Others
• フコキサンチン由来アポカロテノイドによる炎症抑制およびNrf2活性化効果
  高谷直己; 松田慶吾; 別府史章; 細川雅史, 日本水産学会大会講演要旨集(CD-ROM), 2025, 2025
• n-3ドコサペンタエン酸のII型糖尿病/肥満モデルマウスにおける脂質代謝調節作用
  井野友介; 高谷直己; 細川雅史; 別府史章, 日本水産学会大会講演要旨集(CD-ROM), 2025, 2025
• 褐藻フコキサンチンの高齢マウスにおける食餌誘導性肥満に与える影響
  川添朱鞠; 高谷直己; 別府史章; 細川雅史, 日本栄養・食糧学会大会講演要旨集, 78th, 2024
• マクロファージに対するアポ-12’-カプソルビナールの炎症抑制およびNrf2活性化効果
  高谷直己; 宮房拓生; 山野由美子; 別府史章; 細川雅史, 日本栄養・食糧学会大会講演要旨集, 78th, 2024
• Fucoxanthinによる樹状細胞の炎症抑制およびミトコンドリア制御
  七戸優斗; 高谷直己; 別府史章; 細川雅史, 日本水産学会大会講演要旨集(CD-ROM), 2024, 2024

• 脂質・脂肪酸関連物質の使いこなし方 : 素材開発・機能創生・応用技術
  石井, 淑夫, 水産脂質の栄養機能性
  テクノシステム, Sep. 2020, 9784924728868, 3, 15, 592p, Japanese, [Contributor]

• 水産物由来エーテル型脂質の健康機能性
  別府史章
  第6回脂質駆動学術産業創生研究部会, 04 Oct. 2024, Invited oral presentation
  [Invited]
• マウス筋損傷モデルを用いたエーテル型脂質の筋形成制御作用の検討
  今村響介; 高谷直己; 細川雅史; 別府史章
  第62回日本油化学会年会, 03 Sep. 2024, Oral presentation
• 褐藻フコキサンチンの高齢マウスにおける食餌誘導性肥満に与える影響
  川添 朱鞠; 高谷 直己; 別府 史章; 細川 雅史
  日本栄養・食糧学会大会講演要旨集, Apr. 2024, (公社)日本栄養・食糧学会, Japanese
  Apr. 2024 - Apr. 2024
• マクロファージに対するアポ-12'-カプソルビナールの炎症抑制およびNrf2活性化効果
  高谷 直己; 宮房 拓生; 山野 由美子; 別府 史章; 細川 雅史
  日本栄養・食糧学会大会講演要旨集, Apr. 2024, (公社)日本栄養・食糧学会, Japanese
  Apr. 2024 - Apr. 2024
• 北太平洋に分布する動物プランクトン群集の脂質特性分析
  笹川拓真; 山口篤; 高谷直己; 細川雅史; 別府史章
  日本水産学会大会講演要旨集(CD-ROM), 2024
  2024 - 2024
• 海洋細菌が産生する希少カロテノイドの同定と抗炎症活性評価
  高谷直己; 別府史章; 細川雅史
  日本水産学会大会講演要旨集(CD-ROM), 2024
  2024 - 2024
• 函館沿岸で採取したキングサーモンの脂質特性
  李旻軒; 横山楓; 滑げい卓; 高谷直己; 別府史章; 細川雅史
  日本水産学会北海道支部大会講演要旨集, 2024
  2024 - 2024
• 海洋性カロテノイドastaxanthinの生体内代謝物の同定と組織分布
  高谷直己; 向村奏; 別府史章; 細川雅史
  日本水産学会大会講演要旨集(CD-ROM), 2023
  2023 - 2023
• n-3PUFA結合ホスファチジルグリセロールの細胞内脂肪酸調節機構の解明
  富樫咲; 別府史章; 高谷直己; 細川雅史
  日本水産学会大会講演要旨集(CD-ROM), 2023
  2023 - 2023
• 未利用水産生物の可能性を拡げる脂質機能性研究
  別府史章
  日本栄養・食糧学会大会講演要旨集, 2023, Invited oral presentation
  2023 - 2023
• イトマキヒトデ脂質成分によるC2C12細胞の筋管形成促進効果
  別府史章; 福島葵; 高谷直己; 細川雅史
  日本水産学会大会講演要旨集(CD-ROM), 2022
  2022 - 2022
• 水産物由来アルキルグリセロールによるC2C12筋管サイズ増大効果
  佐々木健朗; 亀山美宇; 高谷直己; 細川雅史; 別府史章
  日本栄養・食糧学会大会講演要旨集, 2022
  2022 - 2022
• 海洋性カロテノイドfucoxanthinの新規生体内代謝物の同定と組織分布解析
  高谷直己; 向村奏; 永田航大; 田谷大輔; 別府史章; 細川雅史
  日本栄養・食糧学会大会講演要旨集, 2022
  2022 - 2022
• 糖尿病肥満モデルKK-A^yマウスにおける水産物由来モノアルキル型中性脂質の脂質代謝への影響
  柿本真幸; 高谷直己; 細川雅史; 別府史章
  日本水産学会大会講演要旨集(CD-ROM), 2022
  2022 - 2022
• 水産物由来エーテル脂質の新たな健康機能性を探る
  別府史章
  日本栄養・食糧学会北海道支部シンポジウム講演要旨集, 2022, Invited oral presentation
  2022 - 2022, [Invited]
• ドスイカ(Berryteuthis magister)由来モノアルキル型中性脂質の2型糖尿病モデルKK-A^yマウスに対する脂質代謝への影響評価
  柿本真幸; 高谷直己; 細川雅史; 別府史章
  日本水産学会大会講演要旨集(CD-ROM), 2021
  2021 - 2021
• Exploring the Possibility of a “Copepod Fishery” targeting on the Marine Lower Trophic Organisms
  山口篤; 松石隆; 向井徹; 藤森康澄; 別府史章; 細川雅史
  日本ベントス学会・日本プランクトン学会合同大会講演要旨集, 2021
  2021 - 2021
• 低次栄養段階生物を対象とした「かいあし漁業」の可能性を探る
  山口篤; 松石隆; 向井徹; 藤森康澄; 別府史章; 細川雅史
  日仏海洋学会学術研究発表会講演要旨集, 2021
  2021 - 2021
• フコキサンチン生体内代謝物の合成および構造解析
  向村奏; 永田航大; 高谷直己; 別府史章; 細川雅史
  日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2021
  2021 - 2021
• 高度不飽和ホスファチジルグリセロールの酵素的合成と肝臓脂質低下作用
  陳莉萍; 高谷直己; 別府文章; 宮下和夫; 細川雅史
  日本水産学会大会講演要旨集, 2020
  2020 - 2020
• 低次栄養段階生物を対象とした「かいあし漁業」の可能性を探る
  山口篤; 松石隆; 向井徹; 藤森康澄; 別府史章; 細川雅史
  日本水産学会北海道支部大会講演要旨集, 2020
  2020 - 2020
• Regulation of HDL-quality and muscle function by dietary lipids from marine products
  別府史章
  飯島藤十郎記念食品科学振興財団年報, 2020
  2020 - 2020
• 非アルコール性脂肪肝炎誘導モデルマウスに対する海洋性カロテノイドfucoxanthinの炎症抑制作用
  高谷直己; 河野友香; 別府史章; 宮下和夫; 細川雅史
  日本栄養・食糧学会大会講演要旨集, 2020
  2020 - 2020
• n-3高度不飽和脂肪酸によるC2C12細胞の筋分化能保護効果
  別府史章; 山崎凌平; 宮下和夫; 細川雅史
  日本栄養・食糧学会大会講演要旨集, 2019
  2019 - 2019
• C2C12筋芽細胞におけるEPA/DHAの筋管形成能保護効果
  別府史章; 山崎凌平; 宮下和夫; 細川雅史
  日本油化学会年会講演要旨集, 2019
  2019 - 2019
• ジヒドロキシ脂肪酸による糖尿病/肥満マウスの糖・脂質代謝改善作用
  藤翔太; 別府史章; KIM Hak-Ryul; HOU Ching T.; 宮下和夫; 細川雅史
  日本油化学会年会講演要旨集, 2019
  2019 - 2019
• アカモクからのMCT抽出油の機能性評価
  松本卓己; 手良向一嘉; 別府史章; 細川雅史; 宮下和夫
  日本油化学会年会講演要旨集, 2019
  2019 - 2019
• フコキサンチンのUCP1欠損マウスにおけるミトコンドリア因子の発現誘導
  秋田知輝; 岡松優子; 別府史章; 宮下和夫; 細川雅史
  日本油化学会年会講演要旨集, 2019
  2019 - 2019
• アスタキサンチン由来アポカロテノイドによる炎症抑制機構
  高谷直己; 別府史章; 宮下和夫; 細川雅史
  日本油化学会年会講演要旨集, 2019
  2019 - 2019
• ¹³Cを用いたトリアシルグリセロール脂肪酸結合位置と脂肪酸種の違いが脂肪酸体燃焼性に及ぼす影響の精査
  後藤直宏; 山谷好生; 別府史章; 別府史章; 吉永和明; 吉永和明; 永井利治
  日本油化学会年会講演要旨集, 2019
  2019 - 2019
• ¹³Cを用いた脂肪酸の体燃焼性に及ぼすカルシウム添加効果
  吉永和明; 吉永和明; 山谷好生; 別府史章; 別府史章; 永井利治; 後藤直宏
  日本油化学会年会講演要旨集, 2019
  2019 - 2019
• 海洋生物由来ビタミンEによる脂肪細胞への分化誘導能
  別府史章; 相田祐介; 金子昌弘; 笠谷聡; 青木由典; 後藤直宏
  日本栄養・食糧学会大会講演要旨集, 2018
  2018 - 2018
• RAW264.7細胞におけるテトラコサヘキサエン酸の抗炎症作用評価
  安藤まなみ; 吉永和明; 後藤直宏; 別府史章
  日本油化学会年会講演要旨集, 2018
  2018 - 2018
• マウスの脳におけるドコサテトラエン酸の蓄積機構
  石川榛那; 吉永和明; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2018
  2018 - 2018
• 海洋性ビタミンE(Marine-derived tocopherol)の脂肪細胞分化に対する影響
  相田祐介; 別府史章; 後藤直宏
  日本水産学会大会講演要旨集, 2017
  2017 - 2017
• 海洋性ビタミンE(Marine-derived tocopherol)の体内組織分布に関する研究
  別府史章; 近藤仁美; 後藤直宏
  日本栄養・食糧学会大会講演要旨集, 2017
  2017 - 2017
• 食後高トリグリセライド血症を治療ターゲットとしたメトホルミン投与法の検討
  佐藤大介; 森野勝太郎; 村田幸一郎; 中川史之; 関根理; 別府史章; 後藤直宏; 卯木智; 前川聡
  糖尿病(Web), 2017
  2017 - 2017
• キヒトデ(Aterias amurensis)抽出油による高脂肪食摂取マウスの脂質代謝改善効果
  別府史章; 李皓き; 吉永和明; 永井利治; 吉田明彦; 後藤直宏
  日本水産学会大会講演要旨集, 2017
  2017 - 2017
• 食用油脂中の飽和脂肪酸を結合したトリアシルグリセロール異性体の直接分離
  木下徹亮; 永井利治; 吉田明彦; 吉永和明; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2017
  2017 - 2017
• 油脂中に含まれているラクトン及び遊離脂肪酸の同時定量法の開発
  小尾純志; 吉永和明; 永井利治; 吉田明彦; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2017
  2017 - 2017
• 海洋性ビタミンEの探索と機能性
  別府史章
  日本油化学会年会講演要旨集, 2016
  2016 - 2016
• 海洋性ビタミンE(Marine-derived tocopherol)の組織への蓄積に関する研究
  近藤仁美; 別府史章; 後藤直宏
  日本水産学会大会講演要旨集, 2016
  2016 - 2016
• トランス脂肪酸異性体間の組織への蓄積と異化効率の違い
  別府史章; 浅沼公晴; 河村芳徳; 永井利治; 吉永和明; 溝部帆洋; 小島浩一; 笠谷聡; 永尾晃治; 久保篤史; 神田穣太; 後藤直宏
  日本栄養・食糧学会大会講演要旨集, 2016
  2016 - 2016
• バターの脂肪酸組成および香気成分の季節変動
  吉永和明; 小尾純志; 永井利治; 吉田明彦; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2016
  2016 - 2016
• 油脂中に含まれているラクトンの簡易的分析法の構築
  小尾純志; 吉永和明; 永井利治; 吉田明彦; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2016
  2016 - 2016
• 過酸化脂質がラットの異食性および自発運動量へ与える影響
  別府史章; 北村風季; 渡邊浩幸; 後藤直宏
  日本油化学会年会講演要旨集, 2015
  2015 - 2015
• 鶏卵を構成するトリアシルグリセロール異性体の分析
  後藤直宏; 石川桂子; 永井利治; 吉永和明; 溝部帆洋; 小島浩一; 別府史章
  日本油化学会年会講演要旨集, 2015
  2015 - 2015
• キラルHPLCによる食用油脂トリアシルグリセロール位置異性体・鏡像異性体の同時分離
  永井利治; 石川桂子; 渡邊菜採子; 吉永和明; 溝部帆洋; 小島浩一; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2015
  2015 - 2015
• 乳脂肪を構成するトリアシルグリセロール(TAG)異性体の分析
  渡邊菜採子; 永井利治; 吉永和明; 溝部帆洋; 小島浩一; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2015
  2015 - 2015
• GC-FIDによる食用油脂トリアシルグリセロールの高精度分析法の構築
  吉永和明; 秋山由美; 永井利治; 溝部帆洋; 小島浩一; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2015
  2015 - 2015
• 食用油脂中トリアシルグリセロールにおけるトランス脂肪酸の位置特異的分析
  吉永和明; 河村芳徳; 北山敬; 永井利治; 溝部帆洋; 小島浩一; 渡辺嘉; 佐藤伸一; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 09 Sep. 2014, Japanese
  09 Sep. 2014 - 09 Sep. 2014
• 新規イオン液体カラムSLB-IL111を用いた食用油脂中のシスおよびトランスオクタデセン酸異性体のGC-FID分析
  吉永和明; 浅沼公晴; 永井利治; 溝部帆洋; 小島浩一; 別府史章; 後藤直宏
  日本油化学会年会講演要旨集, 2013
  2013 - 2013
• HPLC-Nano quantity analyte detector(NQAD)を用いたトリアシルグリセロール分子種の新規定量分析方法の開発
  別府史章; 吉永和明; 永井利治; 溝部帆洋; 小島浩一; 後藤直宏
  日本油化学会年会講演要旨集, 2013
  2013 - 2013
• ラット乳脂肪中のトリアシルグリセロール分子種の定量
  後藤直宏; 渡邊菜採子; 永井利治; 吉永和明; 溝部帆洋; 小島浩一; 北村洋平; 清水隆司; 別府史章
  日本油化学会年会講演要旨集, 2013
  2013 - 2013
• LC/MSによる短鎖・中鎖脂肪酸を含む乳脂トリアシルグリセロール位置異性体および鏡像異性体の定量
  永井利治; 渡邊菜採子; 吉永和明; 溝部帆洋; 小島浩一; 別府史章; 齋藤忠夫; 後藤直宏
  日本油化学会年会講演要旨集, 2013
  2013 - 2013
• 褐藻由来フコキサンチンの白色脂肪組織におけるUCP1発現誘導機構
  小出愛; 松本伊代; 別府史章; 前多隼人; 細川雅史; 宮下和夫
  日本農芸化学会大会講演要旨集(Web), 2012
  2012 - 2012
• 褐藻由来フコキサンチンによる肝臓でのSCD1発現抑制作用
  別府史章; 篠田太郎; 細川雅史; 宮下和夫
  日本水産学会大会講演要旨集, 2011
  2011 - 2011
• フコキサンチンによる白色脂肪組織の肥大化抑制機構
  細川雅史; 任美珍; 小出愛; 西川翔; 別府史章; 宮下和夫
  肥満研究, 2011
  2011 - 2011
• フコキサンチンの肥満初期における予防効果と抗炎症作用
  任美珍; 別府史章; 宮下和夫; 細川雅史
  日本農芸化学会大会講演要旨集, 2011
  2011 - 2011
• フコキサンチンによるマクロファージおよびCD8+T細胞の白色脂肪組織への浸潤抑制作用
  細川雅史; 任美珍; 別府史章; 宮下和夫
  肥満研究, 2010
  2010 - 2010
• 9 trans,11 trans型CLAのヒト結腸ガン細胞に対する
  別府史章; 細川雅史; 田中礼央; 宮下和夫
  日本農芸化学会大会講演要旨集, 2005
  2005 - 2005

• Introduction to Fisheries Sciences Ⅰ（水産科学汎論Ⅰ）, 2024年, 修士課程, 水産科学院
• 生物資源化学特論Ⅱ, 2024年, 修士課程, 水産科学院
• 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
• 健康と社会, 2024年, 学士課程, 全学教育
• 環境と人間, 2024年, 学士課程, 全学教育
• 教科教育法（水産Ⅰ）, 2024年, 学士課程, 教育学部
• 生化学Ⅰ, 2024年, 学士課程, 水産学部
• 食品栄養学実験, 2024年, 学士課程, 水産学部

• 2023 - Present
  日本脂質生化学会
• JAPAN SOCIETY OF NUTRITION AND FOOD SCIENCE
• JAPAN OIL CHEMISTS' SOCIETY
• THE JAPANESE SOCIETY OF FISHERIES SCIENCE

• Exploring the Possibility of a "Copepod Fishery" targeting on the Marine Lower Trophic Organisms
  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Pioneering)
  Jul. 2020 - Mar. 2026
  山口 篤; 松石 隆; 向井 徹; 藤森 康澄; 別府 史章
  今年度はかいあし漁業の内容を広く一般に普及させるための情報発信を主に行った。国内学会の発表２件に加え、Fish of the Month (FoM) という、北海道大学水産学部の研究成果を紹介するHPにおいて、本科研費の研究分担者を主とする５名による、かいあし漁業に関する内容を紹介した。紹介内容と紹介者は以下の通りである：「海洋生態系におけるカイアシ類の重要性とかいあし漁業への夢（山口 篤）」、「カイアシ類の音響探知（向井 徹）」、「カイアシ類の音響散乱特性（福田美亮）」、「カイアシ類を対象とした商業漁業（藤森康澄）」、「カイアシ類-新たな水産脂質供給源として（別府史章）」。
  カイアシ類を対象とした研究論文として、北太平洋亜寒帯域に優占する日周鉛直移動を行うカイアシ類Metridia pacificaの一次生産への摂餌インパクトを推定し、呼吸量による輸送量の推定を行った。これは、表層（0-50 m）と深海（50-200 m）の水温条件下において、同種の雌成体を飼育し、呼吸による酸素消費量を測定し、現場の表層における摂餌量の推定と、深海における呼吸による炭素輸送量を推定したものである。夜間の表層における個体数は１平方メートルあたり27～5422個体の間にあり、昼間の表層には本種は出現せず、これは日周鉛直移動の反映と考えられた。日周鉛直移動バイオマスは、１平方メートルあたり1-309 mg Cであった。1日の夜間表層と昼間深海での滞留時間を考慮すると、1日あたり１平方メートルあたりの摂餌量は0.04-11.04 mg Cと推定され、これは現場一次生産量の0-2.4％を占めていた。一方、昼間深海での1日あたり１平方メートルあたりの呼吸量は0.02-9.39 mg Cと計算された。これは有光層からの沈降粒子輸送量の0-10％に相当しており、物質循環における重要性が示された。
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Pioneering), Hokkaido University, 20K20573
• Effect of marine lipids on skeletal muscle function
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  Apr. 2020 - Mar. 2023
  別府 史章
  超高齢社会にある我が国において、生活習慣病や要介護の問題を背景に骨格筋機能の維持は非常に重要な健康課題である。本研究では食品因子によるアプローチを目的とし、特に水産物由来脂質成分の骨格筋機能への有効性を検討している。
  筋分化促進効果を示すイトマキヒトデ脂質のホスファチジルコリン（PC）画分について、PCサブクラス組成比をHPLC分析すると、ジアシル型PCに加え、アルキルアシル型PCが約40%、プラスマローゲン型PCが約10%含まれることが分かった。そこで、これらの活性を比較評価したところ、筋管形成促進効果はサブクラス間で異なることを見出した（未発表、投稿準備中）。また、ヒトデPC処理した細胞では筋管マーカーであるミオシン重鎖の発現増加が認められるとともに、他臓器と連関したエネルギー代謝調節に関わるマイオカインIL-15のmRNA発現量にも増加が認められた。さらに、筋委縮関連遺伝子MuRF1およびatrogin-1 mRNA、これらの発現を制御する転写因子FoxOの核内タンパク質発現量が低下し、ヒトデPCの筋タンパク質代謝制御作用を介した筋委縮予防の有効性が示唆された。一方、対照で用いた大豆PCでは、筋管サイズの増大や関連する遺伝子発現量に変化は認められなかった。以上、本研究ではPCサブクラス間での筋分化における作用の違いが示され、特有な分子構造とその組成を持つヒトデ脂質の優れた健康機能性の新たな可能性が示唆された。
  その他、優れた筋形成促進効果を示す脂質素材を新たに見出し、in vivo試験への研究展開に有用な知見が得られた。
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 20K06221
• Search for new VE homolog and elucidation of its functionalities
  Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
  Apr. 2018 - Mar. 2020
  Beppu Fumiaki
  To clarify the difference in physiological function of vitamin E homologs depending on its structure, we aimed to chemically generate α-, β-, γ-, δ-tocomonoenol (T1), tocodienol (T2) and compared the function in in vitro and in vivo study. We showed that α-, β-, γ-, δ-T1 and T2 can be easily prepared by partial hydrogenation with the corresponding T3, and an analytical method for these minor homologs using LC-MS/MS was established. In diabetic model KK-Ay mice, the protective effect of dietary T1 and T2 on hyperglycemia was observed. We also investigated the effects of minor homologs using various cell models such as cancer and inflammatory macrophage cells. Our results from in vivo and in vitro experiment suggested a difference in the metabolic properties and function due to the side chain structure, providing new insights to nutritional and physiological properties of VE compounds.
  Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, Hokkaido University, Principal investigator, Competitive research funding, 18K14403
• Elucidation of the relationship between the positional isomers of various long-chain monoene-type fatty acids specifically contained in fish oil and their ability to inhibit body fat accumulation
  Grants-in-Aid for Scientific Research
  Apr. 2016 - Mar. 2018
  Gotoh Naohiro
  In this study, the relationship between the structure and health function of long-chain monounsaturated fatty acids (LC-MUFAs), abundant in fish oil, was examined. The position of the double bond was found to affect the function of LC-MUFA because c15-20:1 significantly reduced lipid accumulation in cell experiment among 20:1 isomers consisting of 20 carbon atoms and one double bond. Cell experiments using LC-MUFA with carbons of 18, 20, and 22 revealed that the length of the LC-MUFA carbon chain also affected the health function. Furthermore, when LC-MUFA was mixed with DHA or EPA, “c7-C20:1+DHA” significantly inhibited cholesterol synthesis in the cell compared to “DHA+EPA”.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Tokyo University of Marine Science and Technology, Competitive research funding, 16H03034
• Physiological function of Marine-Derived Tocopherol (MDT)
  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
  Apr. 2014 - Mar. 2017
  Beppu Fumiaki; Gotoh Naohiro
  Physiological function of Marine-Derived Tocopherol (MDT), characteristically occurred in marine organisms was investigated in the present study.
  First, we found that MDT is distributed in whole body tissues although the accumulated amount is less than α-tocopherol. The highest amount of MDT was observed in adipose tissue. Second, MDT enhances the differentiation of 3T3-L1 cells into adipocytes through increases in expression levels of PPARγ and C/EBPα which are key regulators of 3T3-L1 differentiation. Third, MDT reduced production of proinflammatory cytokines both in 3T3-L1 adipocytes and mouse macrophage-like RAW264.7 cells. We further observed the different effects on the activity of 3T3-L1 and RAW264.7 cells among MDT and other vitamin E homologues, suggesting that MDT can regulate cell functions through unique pathways which are different from those of other homologues.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Tokyo University of Marine Science and Technology, Principal investigator, Competitive research funding, 26850132
• フコキサンチンの生体内脂質代謝制御機構の解明
  科学研究費助成事業 特別研究員奨励費
  2011 - 2012
  別府 史章
  ワカメやコンブなどの褐藻類に特徴的なカロテノイドであるフコキサンチン(以下Fx)は、抗肥満効果を有する食品素材として今後の活用が期待されている。本研究ではFxの強い抗肥満作用機構を明らかにすることを目的に、実験動物を用いて脂質代謝制御機構への影響を検討した。
  これまでの研究により、Fxの抗肥満効果にレプチンシグナルが関わっていることを肝臓におけるレプチンのターゲット因子である脂肪酸不飽和化酵素SCD1の発現抑制作用から明らかにした。本研究では、Fxとレプチンシグナルの関連性を明らかにすることを目的とし、レプチンを欠損する肥満ob/obマウスに対するFxの影響を検討した。その結果、KK-Ayマウスで認められた肥満や高血糖の改善効果および肝臓SCD1の発現抑制作用は認められなかった。これらの結果より、Fxの抗肥満効果や血糖値改善効果がレプチン依存的であることを明らかにし、Fxがレプチン抵抗性の改善作用を有する、肥満予防に有望な食品素材であることを示す重要な知見が得られた。
  一方、げっ歯類を用いた試験で報告されているFxの血中コレステロール上昇作用について、これまでに肝臓におけるLDL受容体およびスカベンジャー受容体(SR-B1)を介した、コレステロールおよびリポタンパクの取り込み低下に起因することを示した。さらに本研究では、コレステロール代謝調節に関わる転写因子であるSREBP2の発現上昇と、それにより転写調節を受けるPCSK9の遺伝子発現レベルがFxにより肝臓で増加することを新たに見出した。
  PCSK9はLDL受容体タンパク質分解作用が報告されており、これらがFxによる血中コレステロール上昇作用に一部関わることが明らかとなった。以上の結果は、生体内の脂質運搬を担うリポタンパク質の代謝にもFxが影響することを示唆する重要な知見である。
  日本学術振興会, 特別研究員奨励費, 北海道大学, 11J02867