Researcher basic information

• 博士(歯学)

• https://researchmap.jp/read0046066?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901024859655089
• http://www.den.hokudai.ac.jp/seitaizairyou/index.html
• http://www.okayama-u.ac.jp/user/md/master_program/index.html

• 200901024859655089

• 補綴理工系歯学
• Dental Materials

• Life Science, Prosthodontics
• Life Science, Conservative dentistry

• Bachelor's degree program, School of Dental Medicine
• Doctoral (PhD) degree program, Graduate school of Dental Medicine

Research activity information

• Apr. 2015, 一般社団法人日本歯科理工学会, 平成26年度日本歯科理工学会論文賞
  Ultra-structural evaluation of an anodic oxidated titanium dental implant
  Akiyoshi TAMAGAMI;Noriyuki NAGAOKA;Kumiko YOSHIHARA;Mariko NAKAMURA;Hajime SHIRAI;Takuya MATSUMOTO;Kazuomi SUZUKI;Yasuhiro YOSHIDA
• 2014, 2014 AADR/IADR William J. Gies Award
• 2013, 平成24年度日本歯科理工学会論文賞
• 2010, 第54回日本歯科理工学会学術講演会発表優秀賞
  吉田 靖弘
• 2008, 第1回日本歯科理工学会IADR-DMGC-J記念賞
  Japan
• 2008, Best Presentation Award
  Japan
• 2007, Best Presentation Award at International Dental Materials Congress 2007 in Bankok, Thailand
  Japan
• 2007, 第48回日本歯科理工学会学術講演会発表優秀賞
  吉田 靖弘, Japan
• 2006, 第47回日本歯科理工学会学術講演会発表優秀賞
  吉田 靖弘
• 2005, 第44回日本歯科理工学会学術講演会発表優秀賞
  吉田 靖弘
• 2004, 第43回日本歯科理工学会学術講演会発表優秀賞
  吉田 靖弘
• 2004, Kuraray Young Investigator Award 2004 (2000,- Euro) 1st place winner for the poster (and short oral presentation) on 5th International Kuraray Symposium of Adhesive Dentistry
• 2003, 平成15年度日本歯科保存学会奨励賞
  Japan
• 2003, 平成14年度日本歯科理工学会論文賞
  Japan
• 2003, Dentsply－Merit Award
  Japan
• 2002, GlaxoSmithKline-Award in Odonto-Stomatology 2002
  YOSHIDA Yasuhiro
• 2001, 第4回広島大学歯学部同窓会奨励賞
  Japan
• 1999, Award of the 1st Joint Scientific Meeting between KACD and JSCD
• 1998, Academy of Dental Materials-Fellowship and Student Award

• Development of a novel dismantlable adhesive for orthodontic use triggered by thermal stimulation.
  Ayaka Shundo; Ko Nakanishi; Takayuki Kurokawa; Tomoo Kaneko; Kumiko Yoshihara; Aiko Matsumoto; Ryoshun Endo; Shuhei Otsubo; Takuya Fukuzoe; Tsukasa Akasaka; Yasuhiro Yoshida; Bart Van Meerbeek; Yoshiaki Sato
  Scientific reports, 16, 1, 7041, 7041, 03 Feb. 2026, [International Magazine]
  English, Scientific journal, This study aimed to develop a novel orthodontic adhesive material whose bond strength decreases under external stimuli for easy removal. Stearyl acrylate (SA) and methyl acrylate (MA) copolymerization gels with monomer ratios of 1:1 (SA/MA(1:1) gel) or 3:1 (SA/MA(3:1) gel) were synthesized and added to 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane resin (4META resin) at 30 wt% (4META resin-30% SA/MA(1:1) gel, 4META resin-30% SA/MA(3:1) gel). The thermal and mechanical properties of obtained adhesive were characterized. Bond strength was measured by shear testing at room temperature and at elevated temperatures (50 °C). Transition temperatures of 4META resin-30% SA/MA (1:1) gel and 4META resin-30% SA/MA (3:1) gel were 37.9 °C and 41.6 °C respectively from differential scanning calorimetry measurement. The shear strength of 4META resin, 4META resin-30%SA/MA(3:1) gel, and 4META resin-30%SA/MA(1:1) gel was 8.0 MPa, 8.4 MPa, and 7.1 MPa at room temperature and 6.0 MPa, 3.9 MPa, and 4.7 MPa at 50 °C. In 4META resin containing the copolymerized gel, Young's modulus and tensile strength, as well as shear strength, decreased significantly when the measurement temperature was set to 50 °C from room temperature. A novel adhesive that reduces bond strength by half or less upon temperature stimulation was successfully developed using SA and MA copolymer gels.
• Phosphorylated pullulan as a local drug delivery matrix for cationic antibacterial chemicals to prevent oral biofilm.
  Naoko Namba-Koide; Yasuhiro Yoshida; Noriyuki Nagaoka; Takumi Okihara; Yusuke Kawata; Masahiro Ito; Takashi Ito; Kazu Takeuchi-Hatanaka; Yuki Shinoda-Ito; Kazuhiro Omori; Tadashi Yamamoto; Shogo Takashiba
  BMC oral health, 25, 1, 1333, 1333, 16 Aug. 2025, [International Magazine]
  English, Scientific journal, BACKGROUND: Preventing oral infections, such as oral caries and periodontal disease, helps reduce the risks of various systemic diseases. In this study, the polysaccharide pullulan produced by the black yeast Aureobasidium pullulans was modified in combination with the cationic surfactant cetylpyridinium chloride (CPC) to create a local drug delivery system, and its antibacterial potential on oral bacteria was examined in vitro. METHODS: Pullulan was phosphorylated at the CH2OH residue of α6 in the maltotriose structure and mixed with CPC. Bacterial attachment of cariogenic Streptococcus mutans on hydroxyapatite plates (HAPs) treated with the phosphorylated pullulan (PP) and CPC compound (0.01% PP and 0.001- 0.03% CPC, and vice versa) was assessed by observing bacteria using a field emission scanning electron microscope (FE-SEM) and quantified through 16 S rRNA amplification via real-time polymerase chain reaction (PCR). Additionally, the quartz crystal microbalance (QCM) method was employed to evaluate the sustained release of CPC. RESULTS: PP-CPC compound maintained significant bactericidal activity even at 0.01%, which is one-fifth of the conventional applicable concentration of CPC. Additionally, a residual mixture was detected by the hydroxyapatite sensor of the crystal oscillator microbalance detector, suggesting an unknown molecular interaction that enables the sustained release of CPC after attachment to hydroxyapatite. CONCLUSIONS: The combination of PP and CPC may contribute to the low concentration and effective prevention of oral infections, such as dental caries.
• Development of an Antimicrobial Coating Film for Denture Lining Materials.
  Kumiko Yoshihara; Takeru Kameyama; Noriyuki Nagaoka; Yukinori Maruo; Yasuhiro Yoshida; Bart Van Meerbeek; Takumi Okihara
  Pharmaceutics, 17, 7, 11 Jul. 2025, [International Magazine]
  English, Scientific journal, Background/Objectives: Denture hygiene is essential for the prevention of oral candidiasis, a condition frequently associated with Candida albicans colonization on denture surfaces. Cetylpyridinium chloride (CPC)-loaded montmorillonite (CPC-Mont) has demonstrated antimicrobial efficacy in tissue conditioners and demonstrates potential for use in antimicrobial coatings. In this study, we aimed to develop and characterize CPC-Mont-containing coating films for dentures, focusing on their physicochemical behaviors and antifungal efficacies. Methods: CPC was intercalated into sodium-type montmorillonite to prepare CPC-Mont; thereafter, films containing CPC-Mont were fabricated using emulsions of different polymer types (nonionic, cationic, and anionic). CPC loading, release, and recharging behaviors were assessed at various temperatures, and activation energies were calculated using Arrhenius plots. Antimicrobial efficacy against Candida albicans was evaluated for each film using standard microbial assays. Results: X-ray diffraction analysis confirmed the expansion of montmorillonite interlayer spacing by approximately 3 nm upon CPC loading. CPC-Mont showed temperature-dependent release and recharging behavior, with higher temperatures enhancing its performance. The activation energy for CPC release was 38 kJ/mol, while that for recharging was 26 kJ/mol. Nonionic emulsions supported uniform CPC-Mont dispersion and successful film formation, while cationic and anionic emulsions did not. CPC-Mont-containing coatings maintained antimicrobial activity against Candida albicans on dentures. Conclusions: CPC-Mont can be effectively incorporated into nonionic emulsion-based films to create antimicrobial coatings for denture applications. The films exhibited temperature-responsive, reversible CPC release and recharging behaviors, while maintaining antifungal efficacy, findings which suggest the potential utility of CPC-Mont-containing films as a practical strategy to prevent denture-related candidiasis.
• Pharmacokinetics and the effectiveness of pyrogen-free bioabsorbable wet adhesives.
  Risa Oshima; Kumiko Yoshihara; Ko Nakanishi; Tsukasa Akasaka; Shinji Shimoji; Teppei Nakamura; Takumi Okihara; Mariko Nakamura; Akihiro Matsukawa; Ikkei Tamada; Bart Van Meerbeek; Tsutomu Sugaya; Yasuhiro Yoshida
  Scientific reports, 15, 1, 20056, 20056, 12 Jun. 2025, [International Magazine]
  English, Scientific journal, Bioabsorbable materials are essential for advanced therapies, including surgical sealing, cell therapy, and drug delivery. Natural bioabsorbable materials, including collagen and hyaluronic acid, have better biocompatibility than synthetic bioabsorbable polymers; however, they are mainly derived from animals, presenting infection risks. Non-animal origin polymers have a lower molecular weight than those of animal origins. Their viscosity increases with increase in molecular weight, making endotoxin removal difficult. Here, using the phosphoryl chloride disposal method, we present a strategy for synthesizing pyrogen-free bioabsorbable adhesives with controlled molecular weight. Phosphopullulan, a polysaccharide derivative, had less than detectable endotoxin levels and controllable average molecular weight of approximately 300,000 to over 1,400,000. Furthermore, it is important to ensure the safety as well as efficacy of bio-implantable materials. We have evaluated the biosafety of polysaccharide derivatives we are developing, and have examined their cell phagocytosis and pharmacokinetics in vitro and in vivo, and have confirmed that they are safe. We have also evaluated their adhesion to wet tissue adhesions and confirmed that they leak less than existing materials.
• Top-Down Stereolithography-Based System for Additive Manufacturing of Zirconia for Dental Applications
  Kumiko Yoshihara; Noriyuki Nagaoka; Fiona Spirrett; Yukinori Maruo; Yasuhiro Yoshida; Bart Van Meerbeek; Soshu Kirihara
  Applied Sciences, 15, 11, 6155, 6155, MDPI AG, 30 May 2025
  Scientific journal, This study investigated the feasibility and effectiveness of a commercial top-down stereolithography (SLA)-based system for the additive manufacturing of zirconia dental prostheses. Yttria-stabilized zirconia–resin slurries were prepared, and zirconia objects were fabricated using a top-down SLA system. Thermogravimetric–differential thermal analysis was used to examine the resin, while X-ray fluorescence spectroscopy and X-ray diffraction were used to analyze the printed samples. The microstructures of additively manufactured and subtractively manufactured zirconia were compared using field emission scanning electron microscopy (FE-SEM) before and after sintering. Biaxial flexural strength tests were also conducted to evaluate mechanical properties. The green bodies obtained via additive manufacturing exhibited uniform layering with strong interlayer adhesion. After sintering, the structures were dense with minimal porosity. However, compared to subtractively manufactured zirconia, the additively manufactured specimens showed slightly higher porosity and lower biaxial flexural strength. The results demonstrate the potential of SLA-based additive manufacturing for dental zirconia applications while also highlighting its current mechanical limitations. The study also showed that using a blade to evenly spread viscous slurry layers in a top-down SLA system can effectively reduce oxygen inhibition at the surface and relieve internal stresses during the layer-by-layer printing process, offering a promising direction for clinical adaptation.
• Dual-route administration of balanced anesthesia using medetomidine, midazolam, and butorphanol provides both suitable anesthetic depth and reduced tissue injury in rabbits.
  Risa Iwanaga; Kanako Sumi; Chizuko Kodama; Munekatsu Ita; Mohammad Ibrahim Qasimi; Jun Tamura; Ko Nakanishi; Yasuhiro Yoshida; Masami Morimatsu; Kayoko Matsumura; Teppei Nakamura
  Experimental animals, 74, 2, 181, 188, 20 Apr. 2025, [Domestic magazines]
  English, Scientific journal, Medetomidine, midazolam, and butorphanol (MMB) anesthesia is the preferred choice for rodents but requires excess volume of intramuscular injection in rabbits, which can lead to muscular damage. This study aimed to evaluate a dual-route MMB administration via the intravenous and subcutaneous routes in rabbits. MMB was administered to male Kbs:JW rabbits with an intravenous injection of 0.2 ml/kg followed by a subcutaneous injection of 0.8 ml/kg, totaling 0.2 mg/kg medetomidine, 2.0 mg/kg midazolam, and 2.0 mg/kg butorphanol. We compared the anesthetic effects of this dual-route method with those of intramuscular administration. The dual-route method resulted in a shorter induction time and similar anesthetic duration compared with those of the intramuscular route. While it induced a temporary decrease in body temperature within 30 min post-injection, other vital signs, such as respiration rate, heart rate, and O2 saturation, remained similar. Notably, unlike intramuscular administration, dual-route administration did not increase tissue injury marker levels. This dual-route MMB administration provided sufficient anesthetic depth during surgery, eliminating pain reflexes. Double-dose administration extended anesthetic duration but resulted in rare fatalities, indicating room for protocol improvement. In conclusion, the novel anesthetic method is preferable for injectable anesthesia in rabbits, providing rapid induction and sufficient anesthetic duration, while potentially minimizing muscle injury. This technique may be beneficial for both laboratory and companion animals and significantly enhance animal welfare in anesthesia by reducing the pain associated with injectable anesthesia.
• New future dental material: Antimicrobial material "cetylpyridinium chloride-montmorillonite" and implantable material "phosphorylated pullulan".
  Ko Nakanishi; Tsukasa Akasaka; Yasuhiko Abe; Hiroshi Hayashi; Kumiko Yoshihara; Teppei Nakamura; Mariko Nakamura; Bart VAN Meerbeek; Yasuhiro Yoshida
  Dental materials journal, 44, 1, 1, 8, 11 Jan. 2025, [Domestic magazines]
  English, Scientific journal, In dental practice, there are two major diseases: dental caries and periodontal disease. Although dental treatment techniques have advanced along with advances in dental materials, some diseases such as root surface caries and horizontal bone resorption have not yet achieved satisfactory treatment results. Since these diseases are infections caused by oral bacteria, we believe that materials with long-lasting antimicrobial properties would help control these diseases. In addition, materials that can adhere to wet hard tissues would contribute to treatment. In this review, new materials developed based on this idea, the antimicrobial material "cetylpyridinium chloride-montmorillonite" and the hard tissue adhesive implantable material "phosphorylated pullulan" was introduced.
• Design of bioresorbable calcium phosphate cement with high porosity via the addition of bioresorbable polymers
  Masanobu Kamitakahara; Kakeru Kato; Masaki Umetsu; Kumiko Yoshihara; Yasuhiro Yoshida
  Journal of Biomaterials Applications, 39, 6, 557, 565, SAGE Publications, Jan. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Novel calcium phosphate cements (CPCs) that can be resorbed into the human body need to be developed. One approach for improving bioresorbability is reducing the content of calcium phosphate in CPCs; however, this may induces difficulties in setting the cement and increases the risk of decay. Adding bioresorbable polymers to a liquid solution can shorten the setting time and inhibit decay during setting. A novel bioresorbable polymer, phosphorylated pullulan (PPL), was recently reported. The effect of adding PPL to α-tricalcium phosphate (α-TCP)-based CPCs was examined and compared to that of adding bioresorbable polymers such as collagen, chitosan, and alginate. Collagen did not significantly inhibit the conversion of α-TCP to hydroxyapatite (HA), and its combination with calcium phosphate decreased the setting time and suppressed decay; chitosan decreased the setting time when combined with calcium phosphate; and alginate inhibited the conversion of α-TCP to HA and contributed to suppressing the decay. In contrast, PPL slightly inhibited the conversion of α-TCP to HA; however, its combination with calcium phosphate decreased the setting time. Thus, selecting bioresorbable polymers can help effectively control the properties of CPCs.
• Antibacterial Dental Adhesive Containing Cetylpyridinium Chloride Montmorillonite.
  Yohei Okazaki; Kiichi Nakamori; Chenmin Yao; Mohammed H Ahmed; Benjamin Mercelis; Noriyuki Nagaoka; Yukinori Maruo; Yasuhiro Yoshida; Yasuhiko Abe; Bart Van Meerbeek; Kumiko Yoshihara
  Materials (Basel, Switzerland), 17, 17, 04 Sep. 2024, [International Magazine]
  English, Scientific journal, Oral bacteria cause tooth caries and periodontal disease. Much research is being conducted to prevent both major oral diseases by rendering dental materials' antimicrobial potential. However, such antimicrobial materials are regarded as 'combination' products and face high hurdles for regulatory approval. We loaded inorganic montmorillonite with the antimicrobial agent cetylpyridinium chloride, referred to below as 'CPC-Mont'. CPC-Mont particles in a 1, 3 and 5 wt% concentration were added to the considered gold-standard self-etch adhesive Clearfil SE Bond 2 ('CSE2'; Kuraray Noritake) to render its antibacterial potential (CSE2 without CPC-Mont served as control). Besides measuring (immediate) bonding effectiveness and (aged) bond durability to dentin, the antibacterial activity against S. mutans and the polymerization-conversion rate was assessed. Immediate and aged bond strength was not affected by 1 and 3 wt% CPC-Mont addition, while 5 wt% CPC-Mont significantly lowered bond strength and bond durability. The higher the concentration of the antimicrobial material added, the stronger the antimicrobial activity. Polymerization conversion was not affected by the CPC-Mont addition in any of the three concentrations. Hence, adding 3 wt% CPC-Mont to the two-step self-etch adhesive rendered additional antimicrobial potential on top of its primary bonding function.
• Synthesis and evaluation of fluorescent resins with europium-β-diketonate complex for orthodontic use
  Shuichi YAMAGATA; Takaaki YAMAMOTO; Yoshiaki SATO; Tsukasa AKASAKA; Yasuhiro YOSHIDA
  Dental Materials Journal, 43, 4, 531, 538, Japanese Society for Dental Materials and Devices, 25 Jul. 2024
  Scientific journal
• From Tooth Adhesion to Bioadhesion: Development of Bioabsorbable Putty-like Artificial Bone with Adhesive to Bone Based on the New Material "Phosphorylated Pullulan".
  Ko Nakanishi; Tsukasa Akasaka; Hiroshi Hayashi; Kumiko Yoshihara; Teppei Nakamura; Mariko Nakamura; Bart Van Meerbeek; Yasuhiro Yoshida
  Materials (Basel, Switzerland), 17, 15, 25 Jul. 2024, [International Magazine]
  English, Scientific journal, Bioabsorbable materials have a wide range of applications, such as scaffolds for regenerative medicine and cell transplantation therapy and carriers for drug delivery systems. Therefore, although many researchers are conducting their research and development, few of them have been used in clinical practice. In addition, existing bioabsorbable materials cannot bind to the body's tissues. If bioabsorbable materials with an adhesive ability to biological tissues can be made, they can ensure the mixture remains fixed to the affected area when mixed with artificial bone or other materials. In addition, if the filling material in the bone defect is soft and uncured, resorption is rapid, which is advantageous for bone regeneration. In this paper, the development and process of a new bioabsorbable material "Phosphorylated pullulan" and its capability as a bone replacement material were demonstrated. Phosphorylated pullulan, which was developed based on the tooth adhesion theory, is the only bioabsorbable material able to adhere to bone and teeth. The phosphorylated pullulan and β-TCP mixture is a non-hardening putty. It is useful as a new resorbable bone replacement material with an adhesive ability for bone defects around implants.
• Innovative submucosal injection solution for endoscopic resection with phosphorylated pullulan: a preclinical study.
  Takuya Satomi; Yukari Ochi; Takumi Okihara; Hiroki Fujii; Yasuhiro Yoshida; Katsumi Mominoki; Haruko Hirayama; Junki Toyosawa; Yasushi Yamasaki; Seiji Kawano; Yoshiro Kawahara; Hiroyuki Okada; Motoyuki Otsuka; Akihiro Matsukawa
  Gastrointestinal endoscopy, 99, 6, 1039, 1047, Jun. 2024, [International Magazine]
  English, Scientific journal, BACKGROUND AND AIMS: A submucosal injection solution is used to assist in endoscopic surgery. The high viscosity of current solutions makes them difficult to inject. In the present study, we developed an extremely low-viscosity, easy-to-use submucosal injection solution using phosphorylated pullulan (PPL). METHODS: The PPL solutions were prepared at different concentrations, and their viscosities were measured. The mucosal elevation capacity was evaluated using excised porcine stomachs. Controls included 0.4% sodium hyaluronate (SH), 0.6% sodium alginate (SA), and saline. To evaluate the practicality, the catheter injectability of 0.7% PPL was measured, and EMR and endoscopic submucosal dissection (ESD) were performed using the stomach and colorectum of live pigs. As controls, 0.4% SH and saline were used. RESULTS: The PPL solutions were of extremely low viscosity compared to the solutions of 0.4% SH and 0.6% SA. Nevertheless, the mucosal elevation capacity of PPL solutions for up to 0.7% concentration was similar to that of 0.4% SH, and 0.7% PPL was less resistant to catheter infusion than 0.4% SH and 0.6% SA. In live pig experiments with endoscopic mucosal resection and ESD, snaring after submucosal injection of 0.7% PPL was easier than with 0.4% SH, ESD with 0.7% PPL produced less bubble formation than with 0.4% SH, and the procedure time tended to be shorter with 0.7% PPL than with 0.4% SH because of the shorter injection time. CONCLUSIONS: The PPL solution is an innovative and easy-to-use submucosal injection solution.
• Biological evaluation of novel phosphorylated pullulan-based calcium hydroxide formulations as direct pulp capping materials: An in vivo study on a rat model.
  Md Refat Readul Islam; Rafiqul Islam; Yunqing Liu; Yu Toida; Yasuhiro Yoshida; Hidehiko Sano; Hany Mohamed Aly Ahmed; Atsushi Tomokiyo
  International endodontic journal, 23 May 2024, [International Magazine]
  English, Scientific journal, AIM: Calcium hydroxide (CH) has been considered as a direct pulp capping materials (DPC) for the last decades despite having some limitations. Phosphorylate pullulan (PPL) incorporated with CH (CHPPL) is a novel biomaterial that was introduced as a promising DPC material. Thus, the aim of the study was to evaluate the inflammatory response and mineralized tissue formation (MTF) ability of PPL-based CH formulations on rat molars after DPC. METHODOLOGY: This study consisted of six groups: CH with 1% PPL (CHPPL-1); 3% PPL (CHPPL-3); 5% PPL (CHPPL-5); Dycal and NEX MTA Cement (N-MTA) as the positive control, and no capping materials (NC). One hundred twenty maxillary first molar cavities were prepared on Wistar rats. After capping, all the cavities were restored with 4-META/MMA-TBB resin and pulpal responses were evaluated at days 1, 7, and 28. Kruskal-Wallis followed by Mann-Whitney U-test was performed with a significance level of 0.05. Immunohistochemical expression of IL-6, Nestin, and DMP-1 was observed. RESULTS: At day 1, CHPPL-1, N-MTA, and Dycal exhibited no to mild inflammation, whilst CHPPL-3, CHPPL-5, and NC showed mild to moderate inflammation, and the results were significantly different (p < .05). At day 7, mild to moderate inflammation was observed in CHPPL-1, N-MTA, and Dycal, whereas CHPPL-3, CHPPL-5, and NC exhibited moderate to severe inflammation. Significant differences were observed between CHPPL-1 and N-MTA with NC (p < .05), CHPPL-1 and CHPPL-3 with CHPPL-5 and Dycal (p < .05), and CHPPL-3 with N-MTA (p < .05). A thin layer of mineralized tissue formation (MTF) was observed in all groups. At day 28, CHPPL-1, Dycal, and N-MTA showed no to mild inflammation, whilst CHPPL-3, CHPPL-5, and NC exhibited mild to severe inflammation, and statistically significant difference was detected (p < .05). CHPPL-1, Dycal, and N-MTA exhibited continuous MTF, whilst CHPPL-3, CHPPL-5, and NC had thicker and interrupted MTF. Significant differences were observed between CHPPL-1, CHPPL-3, and N-MTA with NC group (p < .05). Variable expressions of IL-6, Nestin, and DMP-1 indicated differences in the materials' impact on odontoblast-like cell formation and tissue mineralization. CONCLUSIONS: These findings suggest that CHPPL-1 has the potential to minimize pulpal inflammation and promote MTF and had similar efficacy as MTA cement.
• Epithelial-mesenchymal transition in oral cancer cells induced by prolonged and persistent Fusobacterium nucleatum stimulation.
  Shintaro Nakano; Machiko Kasai; Keisuke Nakamura; Tsukasa Akasaka; Yasuhiro Yoshida; Shiho Suzuki; Yoichi Ohiro; Akira Hasebe
  Journal of oral biosciences, 21 May 2024, [International Magazine]
  English, Scientific journal, OBJECTIVES: Several studies have reported the effects of Fusobacterium nucleatum stimulation on oral cancer cells. However, given that these studies typically span a stimulation period of three days to eight days, the in vitro studies conducted to date may not fully mimic the oral cancer environment, which involves constant exposure to oral commensal bacteria. This study aimed to elucidate the effects of prolonged and persistent Fusobacterium nucleatum infection on oral cancer cells. METHODS: Human tongue squamous cell carcinoma (SCC) cells were continuously stimulated with Fusobacterium nucleatum for two or four weeks, then experimentally evaluated. RESULTS: Prolonged, persistent Fusobacterium nucleatum stimulation increased the cells' proliferative, invasive, and migratory capacities, decreased their expression of epithelial markers, and increased their expression of mesenchymal markers progressively with time. The cells also adopted a spindle-shaped morphology and cell-to-cell contact dependence was progressively lost, suggesting time-dependent occurrence of epithelial-mesenchymal transition. Furthermore, mRNA levels of CD44, a cancer stem cell marker, were time-dependently upregulated. When SCC cells were stimulated with Fusobacterium nucleatum for four weeks in the presence of dexamethasone, Fusobacterium nucleatum induced epithelial-mesenchymal transition was inhibited. CONCLUSIONS: Epithelial-mesenchymal transition in human tongue SCC cells was time-dependently induced by prolonged, persistent Fusobacterium nucleatum stimulation and inhibited by dexamethasone. Routine decontamination of the oral cavity may be crucial for controlling tumor invasion and metastasis.
• Investigation of a new implant surface modification using phosphorylated pullulan.
  Kanako Nagamoto; Ko Nakanishi; Tsukasa Akasaka; Shigeaki Abe; Kumiko Yoshihara; Mariko Nakamura; Hiroshi Hayashi; Shinji Takemoto; Masato Tamura; Yoshimasa Kitagawa; Bart Van Meerbeek; Yasuhiro Yoshida
  Frontiers in bioengineering and biotechnology, 12, 1378039, 1378039, 2024, [International Magazine]
  English, Scientific journal, Various implant surface treatment methods have been developed to achieve good osseointegration in implant treatment. However, some cases remain impossible to treat with implants because osseointegration is not obtained after implantation, and the implants fail. Thus, this study focused on phosphorylated pullulan because of its adhesiveness to titanium (Ti) and bone, high biocompatibility, and early replacement with bone. In this study, the response of bone-related cells to phosphorylated pullulan was evaluated to develop a new surface treatment method. Saos-2 (human osteosarcoma-derived osteoblast-like cells), MC3T3-E1 (mouse calvaria-derived osteoblast-like cells), and RAW264.7 (mouse macrophage-like cells) were used. In evaluating cellular responses, phosphorylated pullulan was added to the culture medium, and cell proliferation and calcification induction tests were performed. The proliferation and calcification of cells on the surface of Ti disks coated with phosphorylated pullulan were also evaluated. In addition, bone morphogenetic protein-2 (BMP-2), an osteogenic factor, was used to evaluate the role of phosphorylated pullulan as a drug carrier in inducing calcification on Ti disks. Phosphorylated pullulan tended to promote the proliferation of osteoblast-like cells and the formation of calcification on Ti disks coated with phosphorylated pullulan. Ti disks coated with phosphorylated pullulan loaded with BMP-2 enhanced calcification. Phosphorylated pullulan inhibited osteoclast-like cell formation. These results are due to the properties of phosphorylated pullulan, such as adhesiveness to titanium and drug-loading function. Therefore, phosphorylated pullulan effectively promotes bone regeneration when coated on titanium implants and is useful for developing a new surface treatment method.
• Long-Term Antibacterial Efficacy of Cetylpyridinium Chloride-Montmorillonite Containing PMMA Resin Cement.
  Kumiko Yoshihara; Noriyuki Nagaoka; Yoji Makita; Yasuhiro Yoshida; Bart Van Meerbeek
  Nanomaterials (Basel, Switzerland), 13, 9, 27 Apr. 2023, [International Magazine]
  English, Scientific journal, Despite being able to adhesively restore teeth, adhesives and cement do not possess any anticariogenic protection potential, by which caries recurrence may still occur and reduce the clinical lifetime of adhesive restorations. Several antibacterial agents have been incorporated into dental adhesives and cement to render them anticariogenic. Due to an additional therapeutic effect, such materials are classified as 'dental combination products' with more strict market regulations. We incorporated cetylpyridinium chloride (CPC), often used for oral hygiene applications, into montmorillonite (CPC-Mont), the latter to serve as a carrier for controlled CPC release. CPC-Mont incorporated into tissue conditioner has been approved by the Pharmaceuticals and Medical Devices Agency (PmontMDA) in Japan. To produce a clinically effective dental cement with the antibacterial potential to prevent secondary caries, we incorporated CPC-Mont into PMMA resin cement. We measured the flexural strength, shear bond strength onto dentin, CPC release, and the biofilm-inhibition potential of the experimental CPC-Mont-containing PMMA cement. An 8 and 10 wt% CPC-Mont concentration revealed the antibacterial potential without reducing the mechanical properties of the PMMA cement.
• Antimicrobial adhesive polyurethane gel sheet with cetylpyridinium chloride-montmorillonite for facial and somato prosthesis fastening.
  Kiichi Nakamori; Yasuhiko Abe; Maho Takeuchi; Kazuko Kagawa; Kumiko Yoshihara; Yasuhiro Yoshida; Kazuhiro Tsuga
  Journal of prosthodontic research, 67, 2, 180, 188, 12 Apr. 2023, [Domestic magazines]
  English, Scientific journal, Purpose Existing options for attaching facial and somato prostheses, such as skin adhesives, are problematic because of microbial colonization and skin irritation. This study aims to evaluate the suitability of adhesive polyurethane gel sheets containing cetylpyridinium chloride (CPC)-montmorillonite (Mont) for prosthesis fastening.Methods Adhesive gel sheets were fabricated as mixtures of base resin (99.6 wt% polyol) and hardening agent at a ratio of 3:1 with 0 (control), 2, 5, 10, or 15 wt% CPC-Mont. The controlled release of CPC, antimicrobial activity, in vitro skin irritation, and adhesive force against silicone and human skin at different blending ratios were determined. Statistical analyses of the data were performed using one-way analysis of variance (ANOVA), analysis of covariance (ANCOVA), Tukey's test, or single regression analysis, as appropriate.Results The amount of CPC released increased with the CPC-Mont blending ratio and was linearly proportional to the surface occupation area ratio of CPC-Mont. The samples with >5 wt% CPC-Mont exhibited antimicrobial activity against Staphylococcus aureus at an exposure time of 0 d, and samples with >2 wt% CPC-Mont exhibited antimicrobial activity against Candida albicans at an exposure time of 1 d. All samples were classified as non-irritant based on an in vitro skin irritation test. The adhesive force on the silicone material and human skin decreased with increasing CPC-Mont blending ratio.Conclusions Samples with 5 wt% CPC-Mont are potential candidates as antimicrobial adhesive polyurethane gel sheets for fastening facial and somato prostheses.
• Can a low dosage of recombinant human bone morphogenetic protein-2 loaded on collagen sponge induce ectopic bone?
  Mohammed Katib Alruwaili; Tsutomu Sugaya; Yasuhito Morimoto; Ko Nakanishi; Tsukasa Akasaka; Yasuhiro Yoshida
  Dental materials journal, 42, 3, 433, 440, 07 Apr. 2023, [Domestic magazines]
  English, Scientific journal, Recombinant human bone morphogenetic protein-2 (rhBMP-2) is one of the growth factors that may induce the formation of new bone. The aim was to determine the efficacy of low doses of rhBMP-2 for bone regeneration using a collagen sponge as a carrier. Three doses of rhBMP-2 (1.167, 0.117, and 0.039 mg/mL) were combined with an absorbable collagen sponge (ACS) as a delivery vehicle. The rhBMP-2/ACS implants were placed in the subcutaneous tissues of rat backs. X-ray microcomputed tomography (micro-CT) and histological analysis were used to evaluate bone formation. The samples treated with 1.167 mg/mL of rhBMP-2 showed greater bone formation than the samples treated with 0.117 mg/mL of rhBMP-2 four weeks after surgery. However, there was no evidence of bone formation in the samples that were treated with 0.039 mg/mL of rhBMP-2. It was found that rhBMP-2 was osteogenic even at one-tenth of its manufacturer's recommended concentration (1.167 mg/mL), indicating its potential for clinical use at lower concentrations.
• Acrylamide monomers in universal adhesives
  M. H. Ahmed; K. Yoshihara; N. Nagaoka; C. Yao; A. Matsukawa; Y. Yoshida; B. Van Meerbeek
  Dental Materials, 39, 3, 246, 259, Mar. 2023
  Scientific journal
• Prevention of Root Caries Using Oxalic Acid
  Hidetoshi Oguma; Yasuhiro Matsuda; Kumiko Yoshihara; Katsushi Okuyama; Masahiko Sakurai; Takashi Saito; Satoshi Inoue; Yasuhiro Yoshida
  Materials, 16, 4, 09 Feb. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Certain dentin hypersensitivity treatment materials include oxalic acid to coat dentin surfaces with minerals, while certain organic acids possess a remineralization effect. Herein, an organic acid that inhibits the demineralization and coating of root surfaces was evaluated. Specimens were produced using five non-carious extracted bovines. Four different acids were used: oxalic acid (OA), malonic acid (MA), polyacrylic acid (PA), and succinic acid (SA). Each acid was applied to the root surface and washed using distilled water or a remineralization solution, and the surface was observed using scanning electron microscopy (SEM). All the surfaces of each specimen, barring the polished surface, were covered with wax and immersed in an automatic pH cycling system for two weeks. Dentin demineralization was analyzed using transverse microradiography (TMR) before and after pH cycling. SEM analysis demonstrated that the three acid groups demineralized the dentin surface, whereas the OA group generated crystals covering the dentin surface, even in a distilled water environment. TMR analysis revealed that the OA groups showed significantly lower integrated mineral loss compared with the other groups, even in the distilled water environment. The results suggest that OA generates insoluble calcium oxalate crystals on the dentin and suppresses demineralization even under low saliva conditions.
• Development of Autopolymerizing Resin Material with Antimicrobial Properties Using Montmorillonite and Nanoporous Silica
  Shuhei Otsubo; Ko Nakanishi; Kakufu Fukukawa; Ryoshun Endo; Seiichiro Yoshida; Aiko Matsumoto; Kumiko Yoshihara; Tsukasa Akasaka; Akira Hasebe; Yasuhiro Yoshida; Yoshiaki Sato
  Pharmaceutics, 15, 2, 544, 544, MDPI AG, 06 Feb. 2023, [International Magazine]
  English, Scientific journal, Although autopolymerizing resin offers numerous applications in orthodontic treatment, plaque tends to accumulate between the appliance and the mucosa, which increases the number of microorganisms present. In this study, we added cetylpyridinium chloride (CPC) loaded montmorillonite (Mont) and nanoporous silica (NPS) to autopolymerizing resin (resin-Mont, resin-NPS) and evaluated their drug release capacity, antimicrobial capacity, drug reuptake capacity, mechanical strength, and color tone for the devolvement of autopolymerizing resin with antimicrobial properties. As observed, resin-Mont and resin-NPS were capable of the sustained release of CPC for 14 d, and a higher amount of CPC was released compared to that of resin-CPC. Additionally, resin-Mont and resin-NPS could reuptake CPC. Moreover, the antimicrobial studies demonstrated that resin-Mont and resin-NPS could release effective amounts of CPC against Streptococcus mutans for 14 d and 7 d after reuptake, respectively. Compared to resin-CPC, resin-Mont exhibited a higher sustained release of CPC in all periods, both in the initial sustained release and after reuptake. However, the mechanical strength decreased with the addition of Mont and NPS, with a 36% reduction observed in flexural strength for resin-Mont and 25% for resin-NPS. The application of these results to the resin portion of the orthodontic appliances can prevent bacterial growth on the surface, as well as on the interior, of the appliances and mitigate the inflammation of the mucosa.
• Phosphorylated pullulan promotes calcification during bone regeneration in the bone defects of rat tibiae.
  Yasuhito Morimoto; Tomoka Hasegawa; Hiromi Hongo; Tomomaya Yamamoto; Haruhi Maruoka; Mai Haraguchi-Kitakamae; Ko Nakanishi; Tsuneyuki Yamamoto; Hotaka Ishizu; Tomohiro Shimizu; Kumiko Yoshihara; Yasuhiro Yoshida; Tsutomu Sugaya; Norio Amizuka
  Frontiers in bioengineering and biotechnology, 11, 1243951, 1243951, 2023, [International Magazine]
  English, Scientific journal, The current study aimed to evaluate bone tissue regeneration using a combination of β-tricalcium phosphate (βTCP) and phosphorylated pullulan (PPL, a phosphate-rich polysaccharide polymer consisting of maltotriose units). Round defects of 2 mm diameter were created in the arterial center of rat tibiae, which were further treated with vehicle (control group), βTCP (βTCP group), or βTCP + PPL (βTCP + PPL group) grafts. The control specimens without bone grafts exhibited rapid bone formation after 1 week; however, the regenerated bone was not resorbed until 4 weeks. In contrast, βTCP-grafted specimens exhibited fewer but thicker trabeculae, whereas the βTCP + PPL group displayed many fine trabeculae at 4 weeks. In the βTCP + PPL group, new bone was associated with the βTCP granules and PPL. Similarly, PHOSPHO1-positive osteoblasts were localized on the βTCP granules as well as the PPL. On the other hand, TRAP-reactive osteoclasts predominantly localized on newly-formed bone and βTCP granules rather than on the PPL. No significant differences were observed in the expression of Alp, Integrin αv, Osteopontin, Osteocalcin, and Dmp-1 in PPL-treated MC3T3-E1 osteoblastic cells, suggesting that PPL did not facilitate osteoblastic differentiation. However, von Kossa staining identified abundant needle-like calcified structures extending inside the PPL. Furthermore, transmission electron microscopy (TEM) revealed many globular structures identical to calcified nodules. In addition, calcified collagen fibrils were observed in the superficial layer of the PPL. Thus, PPL may serve as a scaffold for osteoblastic bone formation and promotes calcification on its surface. In conclusion, we speculated that βTCP and PPL might promote bone regeneration and could be integrated into promising osteoconductive materials.
• Osteoclast formation from mouse bone marrow cells on micro/nano-scale patterned surfaces.
  Tsukasa Akasaka; Hiroshi Hayashi; Miho Tamai; Yoshitaka Yoshimura; Yoh-Ichi Tagawa; Hirofumi Miyaji; Ko Nakanishi; Yasuhiro Yoshida
  Journal of oral biosciences, 64, 2, 237, 244, Jun. 2022, [International Magazine]
  English, Scientific journal, OBJECTIVES: Osteoclasts can sense the surface topography of materials. However, it is difficult to identify the structural factors that affect osteoclast formation and its function. Furthermore, we hypothesized that the type of osteoclast precursor cells also affects osteoclastogenesis in the materials. In this study, we investigated the effects of defined micro/nanoscale patterns on osteoclastogenesis from bone marrow cells (BMCs). METHODS: Various cyclo-olefin polymer (COP) patterns were prepared using nanoimprinting. The effects of shape, size, and height of the patterns, and the wettability of the patterned surfaces on osteoclastogenesis from BMCs were evaluated in vitro. RESULTS: Osteoclast formation was promoted on pillars (diameter, 1 μm or 500 nm; height, 500 nm). Notably, osteoclastogenesis from BMCs was better promoted on hydrophobic pillars than on hydrophilic pillars. In contrast, decreased osteoclast formation was observed on the nanopillars (diameter, 100 nm; height, 200 nm). CONCLUSIONS: We demonstrated the promotion of osteoclast formation from BMCs on hydrophobic pillars with diameters of 1 μm and 500 nm. Some cellular behaviors in the patterns were dependent on the type of osteoclast precursor cells. The designed patterns are useful for designing the surface of dental implants or bone replacement materials with a controllable balance between osteoblast and osteoclast activities.
• Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure
  Tsukasa Akasaka; Miho Tamai; Yoshitaka Yoshimura; Natsumi Ushijima; Shinichiro Numamoto; Atsuro Yokoyama; Hirofumi Miyaji; Ryo Takata; Shuichi Yamagata; Yoshiaki Sato; Ko Nakanishi; Yasuhiro Yoshida
  NANO RESEARCH, Jan. 2022
  English, Scientific journal
• Novel composite cement containing the anti-microbial compound CPC-Montmorillonite.
  Yuya Yamamoto; Kumiko Yoshihara; Noriyuki Nagaoka; Bart Van Meerbeek; Yasuhiro Yoshida
  Dental materials : official publication of the Academy of Dental Materials, 38, 1, 33, 43, 17 Dec. 2021, [International Magazine]
  English, Scientific journal, OBJECTIVES: The aim of this study was to evaluate the mechanical properties, bonding performance and anti-microbial activity of a novel composite cement containing cetylpyridinium chloride (CPC) modified montmorillonite ('CPC-Mont'), and using these parameters to determine the optimal particle size and concentration of CPC-Mont the composite cement can be loaded with. METHODS: CPC-Mont particles with a median diameter of 30 and 7 µm were prepared and added to a composite cement at a concentration of 2, 3, 4, 5 and 7.5 wt%. Mechanical properties and bonding performance of the experimental composite cements were evaluated by 3-point bending and micro-tensile bond-strength testing. The amount of CPC released from the cement disks was quantified using a UV-vis recording spectrophotometer. The anti-biofilm activity was studied using scanning electron microscopy (SEM). RESULTS: Adding 30-μm CPC-Mont decreased the mechanical properties and bonding performance of the composite cement, while no reduction was observed for the 7-μm CPC-Mont loaded cement formulation. Although CPC release substantially decreased during the 7-day period assessed, 5- and 7.5-wt% CPC-Mont loaded composite cement inhibited biofilm formation for 30 days. SIGNIFICANCE: Loading composite cement with CPC-Mont with a median diameter of 7 µm at concentrations of 5-7.5 wt% was effective in achieving continuous anti-biofilm activity, while maintaining mechanical strength and bonding performance.
• Ion Capture and Release Ability of Glass Ionomer Cement Containing Nanoporous Silica Particles with Different Pore and Particle Size
  R. Endo; K. Nakanishi; Y. Bando; S. Abe; H. Maruoka; M. Nakamura; T. Akasaka; Y. Yoshida; Y. Sato
  Materials, 14, 19, 5742, 5742, MDPI AG, 01 Oct. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, This study prepared glass ionomer cement (GIC) containing nanoporous silica (NPS) (GIC–NPS) at 5 wt% concentrations using 3 types of NPS with different pore and particle sizes and evaluated the differences in their cationic ion capture/release abilities and mechanical properties. The cationic water-soluble dye was used as cationic ion. The test GIC–NPS complexes captured dyes by immersion in 1 wt% dye solutions. All the GIC–NPS complexes released dyes for 28 d, and the amount of dye released from the complexes increased with decreasing pore size; however, the particle size of NPS did not affect the amount of dye released. Additionally, GIC–NPS was able to recharge the dye, and the amount of released the dye by the complexes after recharge was almost identical to the amount released on the first charge. Although not significantly different, the compressive strength of GIC–NPS was slightly greater than that of GIC without NPS regardless of the type of NPS. These results suggest that the degree of capture and release of cationic molecules, such as drugs, can be controlled by optimizing the pore size of NPS without sacrificing its mechanical strength when its content is 5 wt%.
• Histological evaluation of a novel phosphorylated pullulan-based pulp capping material: An in vivo study on rat molars.
  Rafiqul Islam; Yu Toida; Fei Chen; Toru Tanaka; Satoshi Inoue; Tetsuya Kitamura; Yasuhiro Yoshida; Abu Faem Mohammad Almas Chowdhury; Hany Mohamed Aly Ahmed; Hidehiko Sano
  International endodontic journal, 54, 10, 1902, 1914, Oct. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, AIM: To evaluate the dental pulp response to a novel mineral trioxide aggregate containing phosphorylated pullulan (MTAPPL) in rats after direct pulp capping. METHODS: Ninety-six cavities were prepared in the maxillary first molars of 56 male Wistar rats. The dental pulps were intentionally exposed and randomly divided into four groups according to the application of pulp capping materials: MTAPPL; phosphorylated pullulan (PPL); a conventional MTA (Nex-Cem MTA, NCMTA; positive control); and Super-Bond (SB; negative control). All cavities were restored with SB and observed for pulpal responses at 1-, 3-, 7- and 28-day intervals using a histological scoring system. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney U-test with Bonferroni's correction, and the level of significance was set at 0.05. DMP1 and CD34 antigen were used to evaluate odontoblast differentiation and pulpal vascularization, respectively. RESULTS: On day 1, mild inflammatory cells were present in MTAPPL and NCMTA groups; fewer inflammatory cells were present in the PPL, whereas SB was associated with a mild-to-moderate inflammatory response. A significant difference was observed between PPL and SB (p < .05). No mineralized tissue deposition was observed. On day 3, moderate-to-severe inflammatory cells were present in PPL and SB, whereas MTAPPL and NCMTA had a mild inflammatory response. Initial mineralized tissue deposition was observed in the NCMTA, MTAPPL and SB. A significant difference was observed between MTAPPL and PPL (p < .05). On day 7, a thin layer of mineralized tissue was observed in all tested groups with no or mild inflammatory response. On day 28, no inflammatory response was observed in MTAPPL, whereas NCMTA, PPL and SB had mild inflammatory responses. A significant difference was observed between MTAPPL and SB (p < .05). Complete mineralized tissue barrier formation was observed in MTAPPL, NCMTA and PPL with no significant difference (p > .05). SB exhibited incomplete mineralized tissue barriers, significantly different from NCMTA, MTAPPL and PPL (p < .05). The staining with CD34 was positive in all the groups on all observation days. CONCLUSION: The favourable pulpal responses and induction of mineralized tissue formation associated with MTAPPL indicate its potential application as a direct pulp capping material.
• Pulpal response to mineral trioxide aggregate containing phosphorylated pullulan-based capping material.
  Yu Toida; Shimpei Kawano; Rafiqul Islam; Fu Jiale; Afm A Chowdhury; Shuhei Hoshika; Yasushi Shimada; Junji Tagami; Masahiro Yoshiyama; Satoshi Inoue; Ricardo M Carvalho; Yasuhiro Yoshida; Hidehiko Sano
  Dental materials journal, 41, 1, 126, 133, 01 Oct. 2021, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, This study aimed to evaluate the pulpal responses of monkey's pulp after direct pulp capping (DPC) with the novel mineral trioxide aggregate containing phosphorylated pullulan-based material (MTAPPL). Seventy-two teeth were randomly divided into four groups: MTAPPL; Nex-Cem MTA (NX); TheraCal LC (TH); and Dycal (DY). Histopathological changes in the pulps were observed at days 3, 7 and 70. On day 3, mild inflammatory responses were observed in the MTAPPL, no to moderate inflammatory responses in the TH, whereas moderate inflammatory responses in the NX and DY. No mineralized tissue formation (MTF) was observed in all groups. On day 7, no or mild inflammatory responses were observed in all groups. Initial MTF was observed except for DY. No inflammation with complete MTF including presence of odontoblast-like cells was observed in the MTAPPL, NX and TH groups at day 70. These findings indicate that MTAPPL could be an efficient DPC material.
• Development of new diacrylate monomers as substitutes for Bis-GMA and UDMA.
  Kazuhiko Yoshinaga; Kumiko Yoshihara; Yasuhiro Yoshida
  Dental materials : official publication of the Academy of Dental Materials, 37, 6, e391-e398, Jun. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: Bisphenol A-glycidyl methacrylate (Bis-GMA) and urethane dimethacrylate (UDMA) are widely used as the primary components of (meth)acrylic monomers. However, the use of Bis-GMA, which is a bisphenol A derivative, in dentistry is being questioned after bisphenol A was found to exhibit estrogenic activity. Although UDMA is being considered as a substitute for bis-GMA, the mechanical properties of cured resin composites containing UDMA are less than desirable. Therefore, in this study, we developed new alternative (meth)acrylic monomers to enhance the mechanical strength of cured composite resins. METHODS: Five urethane acrylic monomers were synthesized in this study as (meth)acrylic monomer substituents to replace Bis-GMA and UDMA. The elastic modulus, strength, and breaking energy values of cured resins consisting of mixtures of the urethane acrylates and diluting monomers were determined using the three-point flexural test. The data obtained were analyzed using one-way ANOVA and the post-hoc Tukey HSD tests (p < 0.05). Viscosities of the urethane acrylic monomers were measured with a cone-plate viscometer. Refractive indices of the urethane acrylic monomers were determined with an Abbe refractometer. RESULTS: The results of the three-point flexural tests revealed that the cured urethane acrylic monomer-based resin exhibited higher elastic modulus (up to 40%) and strength (up to 21%) compared to the cured UDMA-based resin. Viscosity and refractive index of the urethane acrylic monomers were between those of UDMA and Bis-GMA. SIGNIFICANCE: The developed urethane diacrylates prepared from diisocyanates which have an aromatic or aliphatic ring, 1,3-bis(1-isocyanato-1-methylethyl)benzene (TMXDI), 1,3-bis(isocyanatomethyl)benzene (XDI), or norbornane diisocyanate (NBDI) are worth considering as alternative options of Bis-GMA and UDMA for restorative resin composites.
• Nano-Layering Adds Strength to the Adhesive Interface.
  K Yoshihara; N Nagaoka; A Nakamura; T Hara; Y Yoshida; B Van Meerbeek
  Journal of dental research, 100, 5, 515, 521, May 2021, [International Magazine]
  English, Scientific journal, X-ray diffraction (XRD) surface analysis and ultrastructural interfacial characterization using transmission electron microscopy (TEM) confirmed that the functional monomer 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) self-assembles into nano-layers at adhesive-tooth interfaces. Self-assembled nano-layering is thought to contribute to the durability of bonding to tooth dentin, although this has not been proven yet. In order to disclose this potential bond-durability contribution of nano-layering, we observed the 3-dimensional (3D) spreading of nano-layering by a series of focused-ion-beam (FIB) milled cross sections by scanning electron microscopy (FIB-SEM) and examined the mechanical properties of self-assembled nano-layering using scanning probe microscopy (SPM). A commercial 10-MDP-containing 3-step self-etch adhesive partially demineralized dentin up to submicron depth, forming a submicron hydroxyapatite-rich hybrid layer. TEM chemically and ultrastructurally confirmed the formation of interfacial nano-layering. FIB-SEM 3D reconstructions disclosed a 3D network of self-assembled nano-layering extending from the hybrid layer up to within the adjacent adhesive-resin layer. SPM revealed that nano-layering within the adhesive-resin layer possessed a higher elastic modulus than that of the surrounding adhesive resin, hereby suggesting that nano-layering contributes to the mechanical strength of adhesives like filler particles do. Nano-layering's 3D expanded structure is expected to strengthen the surrounding resin, as well to better interconnect the adhesive-resin layer to the hybrid layer. In conclusion, this exploratory study demonstrated that nano-layering constitutes a strong phase at the adhesive interface, which may contribute to the clinical longevity of the 10-MDP-based bond to dentin.
• Touch-Cure Polymerization at the Composite Cement-Dentin Interface
  K. Yoshihara; N. Nagaoka; Y. Benino; A. Nakamura; T. Hara; Y. Maruo; Y. Yoshida; B. Van Meerbeek
  Journal of Dental Research, 002203452110010, 002203452110010, SAGE Publications, 26 Mar. 2021
  Scientific journal, Ceramic restorations are often adhesively luted onto the tooth prep. The so-called touch-cure concept was developed to yield optimum polymerization of composite cement at the restoration-cement-tooth interface for immediate bond stabilization. Although this touch cure is theorized to initiate polymerization at the interface when the accelerator in the primer makes contact with the cement, this process has not yet been proven. This study aimed to elucidate the mechanism of touch cure by measuring the degree of conversion (DC) of composite cement applied with or without an accelerator-containing tooth primer (TP) versus an accelerator-free primer using real-time Fourier-transform infrared spectroscopy (RT-FTIR) and attenuated total reflection (ATR)–FTIR. Interfacial bond strength was measured in shear mode, the accelerator composition confirmed by X-ray fluorescence analysis (XRF), and the interfacial interaction of TP and composite cement with dentin investigated by X-ray diffraction (XRD), focused-ion-beam scanning electron microscopy (FIB-SEM) with 3-dimensional interface reconstruction, and transmission electron microscopy (TEM). RT/ATR-FTIR revealed the significantly highest DC when the composite cement was applied with the accelerator-containing primer. XRF disclosed a vanadium compound as a novel chemical accelerator within TP, instead of a classic chemical curing initiator system, to set off touch cure as soon the cement contacts the previously applied primer. Although the TP contains the acidic functional monomer 10-MDP for adhesion to tooth tissue, touch cure using the accelerator-containing TP combined the fastest/highest DC with the highest bond strength. FIB-SEM and TEM confirmed the tight interfacial interaction at dentin with submicron hybridization along with stable 10-MDP also Ca-salt nanolayering.
• Antibacterial Effect of Amino Acid-Silver Complex Loaded Montmorillonite Incorporated in Dental Acrylic Resin.
  Kumiko Yoshihara; Noriyuki Nagaoka; Aya Umeno; Akinari Sonoda; Hideki Obika; Yasuhiro Yoshida; Bart Van Meerbeek; Yoji Makita
  Materials (Basel, Switzerland), 14, 6, 16 Mar. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Several dental materials contain silver for antibacterial effect, however the effect is relatively low. The reason for the lower antibacterial efficacy of silver is considered to be the fact that silver ions bind to chloride ions in saliva. To develop new effective silver antibacterial agents that can be useful in the mouth, we synthesized two novel amino acid (methionine or histidine)-silver complexes (Met or His-Ag) loaded with montmorillonite (Mont) and analyzed their antibacterial efficacy. At first the complexes were characterized using nuclear magnetic resonance (NMR), and amino acid-Ag complex-loaded Mont (amino acid-Ag-Mont) were characterized using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The antibacterial efficacy of these materials in dental acrylic resin was then investigated by bacterial growth measurement using a spectrophotometer. As controls, commercially available silver-loaded zeolite and silver-zirconium phosphate were also tested. Dental acrylic resin incorporating His-Ag-Mont strongly inhibited Streptococcus mutans growth. This was explained by the fact that His-Ag complex revealed the highest amounts of silver ions in the presence of chloride. The structure of the amino acid-Ag complexes affected the silver ion presence in chloride and the antibacterial efficacy. His-Ag-Mont might be used as antibacterial agents for dental materials.
• Cell Adherence Competition between Osteoblasts and Fibroblasts on Various Materials Influences the Establishment of Osseointegration
  Miho Tamai; Kenichi Harimoto; Noriyuki Nagaoka; Kumiko Yoshihara; Yasuhiro Yoshida; Yoh-ichi Tagawa
  Archives of Clinical and Biomedical Research, 05, 04, Fortune Journals, 2021
  Scientific journal
• Three-dimensional observation and analysis of remineralization in dentinal caries lesions
  Kumiko Yoshihara; Noriyuki Nagaoka; Akiko Nakamura; Toru Hara; Satoshi Hayakawa; Yasuhiro Yoshida; Bart Van Meerbeek
  Scientific Reports, 10, 1, 4387, 4387, Springer Science and Business Media LLC, Dec. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The remineralization mechanism in dental caries lesions is not completely understood. This study reports on ultrastructural and chemical changes observed within arrested caries lesions. Carious human teeth were observed using scanning electron microscopy (SEM) and focused-ion-beam (FIB)-SEM. The crystals detected in the caries lesions were characterized by transmission electron microscopy (TEM), along with chemical element mapping using energy-dispersive spectroscopy (EDS)-STEM. FIB-SEM 3D reconstructions revealed a severely damaged dentin surface abundantly covered by bacteria. Although the dentin tubules were clogged up to a depth of 100 μm, bacterial invasion into dentin tubules was not observed. TEM crystal analysis and EDS-STEM revealed the presence of Ca and P, as well as of Mg within the HAp crystals deposited inside the dentin tubules. It was concluded that extensive remineralization with deposition of Mg-HAp crystals had occurred in dentin tubules of caries-arrested dentin. Understanding the natural remineralization process is thought to be helpful for developing clinical biomimetic remineralization protocols.
• Development of tissue conditioner containing cetylpyridinium chloride montmorillonite as new antimicrobial agent: Pilot study on antimicrobial activity and biocompatibility.
  Tsubasa Naoe; Akira Hasebe; Rumi Horiuchi; Yoji Makita; Yohei Okazaki; Keisuke Yasuda; Kenya Matsuo; Yasuhiro Yoshida; Kazuhiro Tsuga; Yasuhiko Abe; Atsuro Yokoyama
  Journal of prosthodontic research, 64, 4, 436, 443, Oct. 2020, [Domestic magazines]
  English, Scientific journal, PURPOSE: The mechanical properties, antimicrobial activity, and biocompatibility of a novel antimicrobial tissue conditioner containing cetylpyridinium chloride with montmorillonite (CPC-Mont) were evaluated. METHODS: To examine the mechanical properties of the novel material, hardness, consistency, and penetration tests were conducted. Antimicrobial activity against Candida albicans (C. albicans) and Staphylococcus aureus (S. aureus) was evaluated. Cell viabilities of fibroblasts and epithelial cells using eluates from materials were measured to evaluate cytotoxicity. In addition, to assess tissue response, animal experiments were conducted. RESULTS: The hardness test results were similar to those of other commercially available materials. The novel tissue conditioner showed good antimicrobial activity against C. albicans and S. aureus compared with other materials. This effect was sustained for a week for C. albicans. In the case of S. aureus, microbial growth was suppressed for up to 3 weeks. Cell viability of the novel material for the eluate at 1 day was significantly less than those of other material for both cells. However, the cell viability at 7 days showed no significant difference. Animal experiments demonstrated that inflammatory responses around materials were not observed on the oral mucosa as other material. CONCLUSION: Within the limitations of this in vitro and in vivo study, the results suggest that the newly developed tissue conditioner containing CPC-Mont has not only excellent antimicrobial properties, but also the same mechanical properties and biocompatibility as tissue conditioners on the market.
• Size- and Morphology-Controlled Preparation of Surface-Modified Water-Dispersible Fullerene Nanoparticles for Bioapplications
  Hidetoshi Oguma; Eri Seitoku; Mami Mutoh; Saori Yoshizawa; Ko Nakanishi; Yosuke Bando; Yuko Era; Takayuki Kiba; Pipop Saikaew; Miho Tamai; Tsukasa Akasaka; Mariko Nakamura; Teruo Kusaka; Yasuhiro Yoshida; Yoshiaki Sato; Hidehiko Sano; Shigeaki Abe; Valanezhad Alireza; Ikuya Watanabe; Satoshi Inoue; Tomoya Takada
  JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 20, 5, 2668, 2674, May 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Silane-coupling effect of a silane-containing self-adhesive composite cement
  Kumiko Yoshihara; Noriyuki Nagaoka; Yukinori Maruo; Goro Nishigawa; Yasuhiro Yoshida; Bart Van Meerbeek
  Dental Materials, 36, 7, 914, 926, Elsevier BV, May 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: Hydrofluoric-acid etching followed by silanization is a routine clinical protocol for durable bonding to glass ceramics. Simplifying ceramic-bonding procedures, new technological developments involve the inclusion of a silane coupling agent in a self-adhesive composite cement. To investigate the effectiveness of the incorporated silane coupling agent, shear bond strength (SB) to ceramic and dentin, contact angle of water (CA), transmission electron microscopy (TEM), X-ray diffraction (XRD) and 29Si nuclear magnetic resonance (NMR) assessments were correlatively conducted. MATERIALS AND METHODS: SB to glass ceramic was measured without ('immediate') and with ('aged') 50K thermocycles upon application of (1) the silane-containing self-adhesive composite cement Panavia SA Cement Universal ('SAU'), being light-cured: 'SAU_light', (2) 'SAU_chem': chemically cured SAU, (3) 'SAP_light': light-cured Panavia SA Cement Plus ('SAP'), and (4) 'SAP_CP': SAP light-cured after separate silanization using Clearfil Ceramic Primer Plus ('CP'). CA was also measured on glass ceramic. The cement pastes before and upon mixing were characterized using 29Si NMR. SB of SAU or SAP onto dentin was measured. Finally, the cement-dentin interface was characterized by TEM and XRD. RESULTS: The immediate and aged SB to glass ceramic of SAU did not significantly differ from those of SAP_CP, while they were significantly higher than those of SAP. CA of SAU did not significantly differ from that of SAP_CP, but it was significantly higher than CA of SAP. 29Si NMR revealed siloxane bonds after mixture. SB of SAU and SAP to dentin did not show any significant difference. SEM, TEM and XRD confirmed tight and chemical interaction, respectively. SIGNIFICANCE: Incorporating silane in a 10-MDP-based self-adhesive composite cement combined efficient silane-coupling ability at the ceramic surface with effective bonding ability at dentin.
• Self-assembled monolayer formation on a dental orthodontic stainless steel wire surface to suppress metal ion elution
  Hironori Tamaki; Shigeaki Abe; Shuichi Yamagata; Yasuhiro Yoshida; Yoshiaki Sato
  Coatings, 10, 4, MDPI AG, 01 Apr. 2020
  English, Scientific journal
• Injectable phosphopullulan-functionalized calcium-silicate cement for pulp-tissue engineering: An in-vivo and ex-vivo study
  Mariano Simón Pedano; Xin Li; Bernardo Camargo; Esther Hauben; Stéphanie De Vleeschauwer; Kumiko Yoshihara; Kirsten Van Landuyt; Yasuhiro Yoshida; Bart Van Meerbeek
  Dental Materials, 36, 4, 512, 526, Elsevier BV, Apr. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To evaluate, by means of an ex-vivo human tooth-culture model and in-vivo minipig animal study, the pulpal inflammatory reaction and reparative dentin-formation capacity of an injectable phosphopullulan-based calcium-silicate cement (GC, Tokyo, Japan) upon pulp capping, this in comparison with the commercial reference material Biodentine (Septodont). METHODS: For the ex-vivo tooth model, 9 freshly-extracted teeth from 3 different patients were pulp-capped with the experimental biomaterial (n = 3), Biodentine (n = 3) or left uncapped (control; n = 3). The teeth were kept in fresh culture medium for 4 weeks and, upon fixation three-dimensional Micro-CT and histology were performed. For the in-vivo animal study, 40 teeth from 3 minipigs were exposed and pulp capped with the experimental biomaterial containing phosphopullulan (n = 24) or Biodentine (n = 16) for 7 or 70 days. The inflammatory reaction and the tissue-regenerative potential was qualitatively and semi-quantitatively characterized using three-dimensional micro-CT and histology. RESULTS: Ex vivo, the treatment with the experimental phosphopullulan-based calcium-silicate cement and Biodentine stimulated the formation of fibrous tissue and mineralized foci. In vivo, early inflammatory reaction and regeneration of the pulp-tissue interface was promoted by both bioceramic materials after 7 and 70 days, respectively. SIGNIFICANCE: Our findings bring new insights into calcium-silicate-mediated dental pulp repair and regeneration. The novel ready-to-use and self-adhering functionalized calcium-silicate cement revealed effective pulpal repair potential.
• Morphology and fluorescence of Y2O3:Eu³⁺ nanophosphors and dispersibility in adhesives resins
  Shuichi Yamagata; Takaaki Yamamoto; Yoshiaki Sato; Natsumi Ushijima; Tsukasa Akasaka; Yasuhiro Yoshida
  Nano Biomedicine, 11, 2, 57, 64, 2020
  Scientific journal
• Fluorescence mechanical properties of Y2O3:Eu³⁺ and Nanophosphor-containing adhesive resins
  Shuichi Yamagata; Takaaki Yamamoto; Yoshiaki Sato; Natsumi Ushijima; Tsukasa Akasaka; Yasuhiro Yoshida
  Nano Biomedicine, 12, 1, 19, 26, 2020
  Scientific journal
• Proliferation of Saos-2 and Ca9-22 cells on grooved and pillared titanium surfaces.
  Naoyuki Kaga; Tsukasa Akasaka; Takashi Matsuura; Atsuro Yokoyama; Yasuhiro Yoshida
  Bio-medical materials and engineering, 30, 5-6, 559, 567, 2020, [International Magazine]
  English, Scientific journal, BACKGROUND: Surface nanostructures in titanium (Ti) oral implants are critical for rapid osseointegration. OBJECTIVE: The purpose of this study was to evaluate the growth of osteoblast-like (Saos-2) and epithelial-like (Ca9-22) cells on nanopatterned Ti films. METHODS: Ti films with 500 nm grooves and pillars were fabricated by nanoimprinting, and seeded with Saos-2 and Ca9-22 cells. Cell viability and morphology were assessed by cell proliferation assay and scanning electron microscopy, respectively. RESULTS: As assessed after 1 hour, proliferation of Saos-2 cells was most robust on grooved films than on pillared and smooth films, in this order. These cells approximately doubled on grooved and pillared substrates in 24 hours and after 5 days, but not on smooth surfaces. In contrast, Ca9-22 cells favored smooth surfaces, followed by grooved and pillared films. Indeed, cells sparsely adhered to pillared films over 5 days of incubation (p < 0.05). CONCLUSIONS: The data show that Saos-2 and Ca9-22 cells respond differently to different nanostructures, and highlight the potential use of nanopatterns to promote bone regeneration or to prevent epithelial downgrowth at the implant-bone interface.
• Proliferation of Saos-2 and Ca9-22 cells on grooved and pillared titanium surfaces
  Naoyuki Kaga; Tsukasa Akasaka; Takashi Matsuura; Atsuro Yokoyama; Yasuhiro Yoshida
  BIO-MEDICAL MATERIALS AND ENGINEERING, 30, 5-6, 559, 567, 2020, [International Magazine]
  English, Scientific journal
• From Buonocore's Pioneering Acid-Etch Technique to Self-Adhering Restoratives. A Status Perspective of Rapidly Advancing Dental Adhesive Technology.
  Bart Van Meerbeek; Kumiko Yoshihara; Kirsten Van Landuyt; Yasuhiro Yoshida; Marleen Peumans
  The journal of adhesive dentistry, 22, 1, 7, 34, 2020, [International Magazine]
  English, Scientific journal, This literature-based OPINION PAPER reflects in an introductory historical perspective on the rapid advancement of dental adhesive technology. Past and current techniques to bond to tooth tissue, in particular to dentin as the most challenging bonding substrate, are critically appraised. Including the historical perspective in (1), this paper focuses on fourteen items thought to be of primary importance with regard to the current status of dental adhesive technology. In (2) the primary mechanisms involved in adhesion to enamel and especially dentin are dealt with having (3) also revisited the previously introduced adhesion-decalcification concept (AD concept) as basis of biomaterial-hard tissue interaction; the worldwide accepted classification of today's adhesives into etch&rinse (E&R) and self-etch (SE) adhesives are presented in (4), along with presentation of their respective PLUS-MINUS balances in (5) and (6); nomination of the GOLD-STANDARD E&R (7) and SE (8) adhesives is based on evidence of successful laboratory and long-term clinical performance, resulting in a recommended 3-step full E&R bonding route in (9) and the preferred 3-step combined selective enamel E&R with 2-SE bonding route in (10); (11) description of the main bond-degradation pathways and eight strategies to preserve bond stability; (12) coverage of the PROS and CONS of the newest generation of UNIVERSAL adhesives. Looking into the future, some expected future developments in dental adhesive technology have been suggested in (13), along with (14) a first status determination of the latest research-and-development towards self-adhesive restorative materials that no longer require any pre-treatment.
• Development of self-adhesive pulp-capping agents containing a novel hydrophilic and highly polymerizable acrylamide monomer
  Kumiko Yoshihara; Noriyuki Nagaoka; Takumi Okihara; Masao Irie; Akihiro Matsukawa; Mariano Simón Pedano; Yukinori Maruo; Yasuhiro Yoshida; Bart Van Meerbeek
  Journal of Materials Chemistry B, 8, 24, 5320, 5329, Royal Society of Chemistry (RSC), 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal,

  Several studies have shown the clinical success of hydraulic calcium-silicate cements (hCSCs) for direct and indirect pulp capping and root repair.

• Atomic level observation and structural analysis of phosphoric-acid ester interaction at dentin.
  Yoshihara K; Nagaoka N; Yoshida Y; Van Meerbeek B; Hayakawa S
  Acta biomaterialia, 97, 544, 556, Oct. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The functional monomer 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP), used in many dental adhesives, self-assembles in nano-layers at adhesive-tooth interfaces. Recently, several states of the POH groups of 10-MDP_Ca salts were suggested, while their actual status has not been elucidated yet. We mechanistically investigated 10-MDP_Ca-salt nano-layering at adhesive-dentin interfaces, correlatively using scanning transmission electron microscopy with energy-dispersive X-ray spectrometry (STEM-EDS), X-ray diffraction (XRD) and solid state nuclear magnetic resonance (NMR). STEM-EDS confirmed the presence of Ca and P in each nano-layer. Both XRD and NMR revealed that the two terminal POH groups of 10-MDP reacted with Ca. This twofold POH interaction of 10-MDP with Ca was stable in water and is therefore expected to contribute to durable nano-layering of 10-MDP_Ca salts in the hybrid and adhesive layer. STATEMENT OF SIGNIFICANCE: The functional monomer 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP), commonly used in dental adhesives with favorable long-term clinical outcome, has been documented to self-assemble into nano-layers at adhesive-tooth interfaces. Characterizing ultra-morphologically (STEM) and chemically (STEM-EDS, XRD, NMR) the mechanisms of interaction of 10-MDP with bulk dentin in a similar manner as what occurs clinically, it was found that the water stable 10-MDP_Ca salts consist of CaRPO4, meaning that the two OH groups of the phosphate group of 10-MDP ionically reacted with Ca. This stable structure is expected to contribute to durable nano-layering of 10-MDP_Ca salts in the hybrid and adhesive layer and hence to clinical longevity of the adhesively bonded tooth restoration.
• Rechargeable anti-microbial adhesive formulation containing cetylpyridinium chloride montmorillonite.
  Matsuo K; Yoshihara K; Nagaoka N; Makita Y; Obika H; Okihara T; Matsukawa A; Yoshida Y; Van Meerbeek B
  Acta biomaterialia, 100, 388, 397, Sep. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Long-term anti-bacterial effect is a desired ability of any dental material in combating tooth caries as one of the most common and widespread persistent diseases today. Among several cationic quaternary ammonium compounds with antiseptic properties, cetylpyridinium chloride (CPC) is often used in mouthrinses and toothpastes. In this study, we incorporated CPC in a soft phyllosilicate mineral (clay), referred to as montmorillonite (Mont), to enable gradual CPC release with rechargeability. Besides measuring CPC release and recharge, we examined the anti-bacterial effect, cytotoxicity and bonding effectiveness of five experimental adhesive formulations, prepared by adding 1 and 3 wt% CPC_Mont, 3 wt% Mont (without CPC), and 1 and 3 wt% CPC (without Mont) to the commercial adhesive Clearfil S3 Bond ND Quick ('C-S3B'; Kuraray Noritake). Strong inhibition of Streptococcus mutans biofilm formation by CPC_Mont adhesives was confirmed by optical density and SEM. CPC release from CPC_Mont adhesives was higher and lasted longer than from CPC adhesives, while CPC_Mont adhesives could also be recharged with CPC upon immersion in 2 wt% CPC. In conclusion, CPC_Mont technology rendered adhesives anti-bacterial properties with recharge ability, this without reducing its bonding potential, neither increasing its cytotoxicity. STATEMENT OF SIGNIFICANCE: Dental caries is one of the most prevalent chronic diseases in the population worldwide and is the major cause of tooth loss. In this study, we developed cetylpyridinium chloride (CPC) loaded montmorillonite (CPC-Mont) with a long-term antibacterial efficacy to prevent caries. CPC is an antibacterial agent approved by FDA, used as an OTC drug and contained in oral hygiene aids. CPC-Mont was incorporated in a dental adhesive to gradually release CPC. CPC_Mont technology rendered adhesives anti-bacterial properties with rechargeability, this without reducing its bonding potential, neither increasing its cytotoxicity.
• Fluorine distribution from fluoride-releasing luting materials into human
  Katsushi Okuyama; Yasuhiro Matsuda; Hiroko Yamamoto; Yukimichi Tamaki; Takashi Saito; Mikako Hayashi; Yasuhiro Yoshida; Hidehiko Sano; Takahiro Sato; Masashi Koka
  Nuclear Instruments and Methods in Physics Research Section B, 456, 16, 20, Jul. 2019, [Peer-reviewed]
  English, Scientific journal
• Fusion of RAW 264.7 macrophage cells on micro-scale fine pillar patterns
  J Honma; T Akasaka; M Tamai; Y Yoshimura; T Taira; H Miyaji; S Yamagata; Y Sato; Y Yoshida
  Dig J Nanomater Bios, 13, 4, 1123, 1131, Dec. 2018, [Peer-reviewed]
  English, Scientific journal
• Ultrastructure and bonding properties of tribochemical silica-coated zirconia.
  Nagaoka N; Yoshihara K; Tamada Y; Yoshida Y; Meerbeek BV
  Dental materials journal, 38, 1, 107, 113, Oct. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Tribochemical silica coating (TSC) is commonly used to pretreat zirconia surfaces prior to luting. Although many studies demonstrate an adhesion-promoting effect of TSC on zirconia, its actual interaction mechanism has not been fully elucidated. We therefore characterized the ultrastructure of TSC-treated zirconia and tested shear-bond strength. STEM/EDS disclosed a micro-roughened zirconia surface partially covered with fused Al and Si, while residual unfused silica particles could also still be detected. TSC-treated zirconia having received the solely silane primer exhibited a significantly lower shear-bond strength than zirconia on which the combined 10-MDP/silane primer was applied. SEM fracture analysis revealed residual silica particles on both the zirconia and cement sides. Correlative ultrastructural and chemical surface characterization revealed that TSC deposited an inhomogeneous silica layer on the zirconia surface, which explains why the solely silane coupling agent was less effective than the combined 10-MDP/silane ceramic primer for bonding to zirconia pretreated by TSC.
• Etching Efficacy of Self-Etching Functional Monomers.
  Yoshihara K; Hayakawa S; Nagaoka N; Okihara T; Yoshida Y; Van Meerbeek B
  J Dent Res., 97, 9, 1010, 1016, Sep. 2018, [Peer-reviewed]
  English
• Differences in interleukin-1β release-inducing activity of Candida albicans toward dendritic cells and macrophages
  Akira Hasebe; Ayumi Saeki; Yasuhiro Yoshida; Ken-ichiro Shibata
  Archives of Oral Biology, 93, 115, 125, Elsevier Ltd, 01 Sep. 2018, [Peer-reviewed]
  English, Scientific journal
• Optical and Chromatic Evaluation of Fluorescent Orthodontic Bonding Materials Doped with a Europium β-diketonate Complex
  Aiko MATSUMOTO; Shuichi YAMAGATA; Shigeaki ABE; Tsukasa AKASAKA; Yasuhiro YOSHIDA; Junichiro IIDA
  Nano Biomedicine, 10, 1, 3, 8, Aug. 2018, [Peer-reviewed]
• Effect of different remaining dentin thickness and long term water storage on dentin bond strength.
  Ting S; Chowdhury AFMA; Sun J; Kakuda S; Sidhu SK; Yoshida Y; Selimovic D; Sano H
  Dental materials journal, 37, 4, 562, 567, Jul. 2018, [Peer-reviewed]
• Chemical interaction of glycero-phosphate dimethacrylate (GPDM) with hydroxyapatite and dentin
  Kumiko Yoshihara; Noriyuki Nagaoka; Satoshi Hayakawa; Takumi Okihara; Yasuhiro Yoshida; Bart Van Meerbeek
  Dental Materials, 34, 7, 1072, 1081, Elsevier Inc., 01 Jul. 2018, [Peer-reviewed]
  English, Scientific journal
• Effect of a nano-scale fine hole pattern on the differentiation of RAW264.7 cells into osteoclasts
  R Takata; T Akasaka; M Tamai; Y Yoshimura; T Taira; H Miyaji; Y Tagawa; S Yamagata; J Iida; Y Yoshida
  Dig J Nanomater Bios, 13, 2, 451, 458, Apr. 2018, [Peer-reviewed]
  English, Scientific journal
• Effects of Lectins on initial attachment of cariogenic Streptococcus mutans
  Takashi Ito; Yasuhiro Yoshida; Yasuyoshi Shiota; Yuki Ito; Tadashi Yamamoto; Shogo Takashiba
  Glycoconjugate Journal, 35, 1, 41, 51, Springer New York LLC, 01 Feb. 2018, [Peer-reviewed]
  English, Scientific journal
• Collagen-Binding Hepatocyte Growth Factor (HGF) alone or with a Gelatin- furfurylamine Hydrogel Enhances Functional Recovery in Mice after Spinal Cord Injury.
  Kentaro Yamane; Tetsuro Mazaki; Yasuyuki Shiozaki; Aki Yoshida; Kensuke Shinohara; Mariko Nakamura; Yasuhiro Yoshida; Di Zhou; Takashi Kitajima; Masato Tanaka; Yoshihiro Ito; Toshifumi Ozaki; Akihiro Matsukawa
  Scientific reports, 8, 1, 917, 917, 17 Jan. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The treatment of spinal cord injury (SCI) is currently a significant challenge. Hepatocyte growth factor (HGF) is a multipotent neurotrophic and neuroregenerative factor that can be beneficial for the treatment of SCI. However, immobilized HGF targeted to extracellular matrix may be more effective than diffusible, unmodified HGF. In this study, we evaluated the neurorestorative effects of an engineered HGF with a collagen biding domain (CBD-HGF). CBD-HGF remained in the spinal cord for 7 days after a single administration, while unmodified HGF was barely seen at 1 day. When a gelatin-furfurylamine (FA) hydrogel was applied on damaged spinal cord as a scaffold, CBD-HGF was retained in gelatin-FA hydrogel for 7 days, whereas HGF had faded by 1 day. A single administration of CBD-HGF enhanced recovery from spinal cord compression injury compared with HGF, as determined by motor recovery, and electrophysiological and immunohistochemical analyses. CBD-HGF alone failed to improve recovery from a complete transection injury, however CBD-HGF combined with gelatin-FA hydrogel promoted endogenous repair and recovery more effectively than HGF with hydrogel. These results suggest that engineered CBD-HGF has superior therapeutic effects than naïve HGF. CBD-HGF combined with hydrogel scaffold may be promising for the treatment of serious SCI.
• Fabrication of light-curing type fluorescent orthodontic bonding materials using europium-ß-diketonate complex
  Shuichi Yamagata; Takaaki Yamamoto; Yoshiaki Sato; Shigeaki Abe; Tsukasa Akasaka; Yasuhiro Yoshida
  Nano Biomedicine, 10, 2, 53, 60, 2018
  Scientific journal
• Chemical modification to suppress metal ions elution of dental orthodontic wire surface
  Hironori Tamaki; Satoshi Tsuchiya; Yuka Sakurada; Shuichi Yamagata; Atsushi Hyono; Kotaro Kaneko; Koji Kawai; Shigeaki Abe; Yasuhiro Yoshida; Junichiro Iida
  MOLECULAR CRYSTALS AND LIQUID CRYSTALS, 660, 1, 163, 172, 2018
  English, Scientific journal
• Electric Charge Dependence of Controlled Dye-Release Behavior in Glass Ionomer Cement Containing Nano-Porous Silica Particles
  Yosuke Bando; Ko Nakanishi; Shigeaki Abe; Shuichi Yamagata; Yasuhiro Yoshida; Junichiro Iida
  JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 18, 1, 75, 79, Jan. 2018, [Peer-reviewed]
  English, Scientific journal
• Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants.
  Reika Makita; Tsukasa Akasaka; Seiichi Tamagawa; Yasuhiro Yoshida; Saori Miyata; Hirofumi Miyaji; Tsutomu Sugaya
  Beilstein journal of nanotechnology, 9, 1735, 1754, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Background: Collagen is a basic component of the periodontium and plays an important role in the function of the periodontal unit. Therefore, coating with collagen/gelatin has been applied to enable dental implants to positively interact with peri-implant tissues. Although the micro/nanoscale topography is an important property of the surface of dental implants, smaller collagen/gelatin surface patterns have not been sufficiently developed. Furthermore, only few reports on the behavior of cells on gelatin surfaces with different patterns and sizes exist. In this study, we developed micro/nanometer-scaled gelatin surfaces using genipin crosslinking, with the aim of understanding the use of patterning in surface modification of dental implants. Results: Grooves, holes, and pillars, with widths or diameters of 2 µm, 1 µm, or 500 nm were fabricated using a combination of molding and genipin crosslinking of gelatin. The stability of the different gelatin patterns could be controlled by the degree of genipin crosslinking. The gelatin patterns at 20 mM concentration of genipin and 41% crosslinking maintained a stable, patterned shape for at least 14 days in a cell culture medium. A cell morphology study showed that the cells on groves were aligned along the direction of the grooves. In contrast, the cells on pillars and holes exhibited randomly elongated filopodia. The vinculin spots of the cells were observed on the top of ridges and pillars or the upper surface of holes. The results of a cell attachment assay showed that the number of surface-attached cells increased with increasing patterning of the gelatin surface. Unlike the cell attachment assay, the results of a cell proliferation assay showed that Saos-2 cells prefer grooves with diameters of approximately 2 µm and 1 µm and pillars with diameters of 1 µm and heights of 500 nm. The number of cells on pillars with heights of 2 µm was larger than those of the other gelatin surface patterns tested. Conclusion: These data support that a detailed design of the gelatin surface pattern can control both cell attachment and proliferation of Saos-2 cells. Thus, gelatin surfaces patterned using genipin crosslinking are now an available option for biocompatible material patterning.
• Titanium implant functionalization with phosphate-containing polymers may favour in vivo osseointegration
  Marcio V. Cardoso; Judith De Rycker; Amol Chaudhari; Eduardo Coutinho; Yasuhiro Yoshida; Bart Van Meerbeek; Marcelo F. Mesquita; Wander J. Da Silva; Kumiko Yoshihara; Katleen Vandamme; Joke Duyck
  JOURNAL OF CLINICAL PERIODONTOLOGY, 44, 9, 950, 960, Sep. 2017, [Peer-reviewed]
  English, Scientific journal
• Interfacial interactions of bioadhesive materials with human hard tissues
  Yoshida, Yasuhiro; Inoue, Satoshi; Tamai, Miho; Abe, Shigeaki; Akasaka, Tsukasa; Yoshihara, Kumiko; Meerbeek, Bart Van
  Hokkaido Journal of Dental Science, 38, 86, 98, 北海道歯学会, Sep. 2017, [Peer-reviewed]
  English, Scientific journal, Recently, in dental practice, tooth reconstruction can be performed using dental adhesive technology following either a "glass-ionomer", an "etch-and-rinse" or a "self-etch" approach. Glass-ionomer cements are well-known to possess an auto-adhesive capability without requiring any surface pre-treatment. The fundamental bonding mechanism of resin-based materials to enamel and dentin is essentially based on an exchange process, in which minerals removed from the dental hard tissues are replaced by resin monomers. These resin monomers, upon polymerization become micro-mechanically interlocked in the created porosities. Besides micro-mechanical interlocking through hybridization, an additional chemical interaction between functional monomers/polymers and tooth substrate components has been found to be important. In this review, we focus on how the chemical interaction of the biomaterial-hard tissue interface can improve bond durability, especially using chemical analytical techniques.
• Y2O3:Eu3+ Phosphors by Homogeneous Precipitation and Subsequent Calcination at Varied Temperatures for Orthodontic Adhesives
  Shuichi YAMAGATA; Takaaki YAMAMOTO; Yoshiaki SATO; Natsumi USHIJIMA; Shigeaki ABE; Tsukasa AKASAKA; Yasuhiro YOSHIDA; Junichiro IIDA
  Nano Biomedicine, 9, 1, 15, 22, Jun. 2017, [Peer-reviewed]
  English, Scientific journal
• Bacterial adhesion not inhibited by ion-releasing bioactive glass filler
  Kumiko Yoshihara; Noriyuki Nagaoka; Yukinori Maruo; Hidehiko Sano; Yasuhiro Yoshida; Bart Van Meerbeek
  DENTAL MATERIALS, 33, 6, 723, 734, Jun. 2017, [Peer-reviewed]
  English, Scientific journal
• Cell adhesion and alignment of human osteoblasts and human gingival fibroblasts on micro/nano-grooved gelatin sheet
  Naoyuki Kaga; Tsukasa Akasaka; Rumi Horiuchi; Yasuhiro Yoshida; Atsuro Yokoyama
  Digest Journal of Nanomaterials and Biostructures, 12, 2, 431, 440, May 2017, [Peer-reviewed]
  English, Scientific journal
• Submicro-patterning of curable dental materials by molding methods: a screening trial
  T Akasaka; H Miyaji; T Imamura; N Kaga; A Yokoyama; Y Yoshida
  Dig J Nanomater Bios, 12, 2, 281, 292, Forum of Chalcogeniders, Apr. 2017, [Peer-reviewed]
  English, We investigated patterning with curable dental materials via micro/nano-molding as ascreening trial. We used typical curable dental materials such as a composite resin (CR),acrylic resin, varnish, adhesive resin, restoration, sealer, cement, tooth desensitizer, andgypsum with either a cyclo-olefin polymer (COP) mold or polydimethylsiloxane (PDMS)mold that had holes, pillars, and grooves with 0.5 μm diameters and 1 μm heights. TheCOP mold was suitable for patterning of holes and grooves with Bondfill SB after 2 hcuring, while PDMS mold was suitable for patterning of pillars with Bondfill SB at the 0.5μm scale after 2 h curing. Most of the curable dental materials could be patterned using theCOP or the PDMS mold. However, dental impression materials could not be patterned asrequired. In addition, the COP mold was suitable for many organic pillars, while thePDMS mold was suitable for inorganic pillars. Further, we achieved patterning on thecurved surface of an artificial tooth with flowable CR using the COP thin film mold. Weconclude that for the formation of uniform shaped patterns, it is important to cure within asuitable time and to select mold types depending on the type of curable dental material.
• Estimation of Controlled Drug-release Behavior of Nano-porous Silica Micro Particles and Their Biocompatibility
  Eri SEITOKU; Yuko ERA; Ko NAKANISHI; Yosuke BANDO; Shinichi KAKUDA; Koichi NAKAMURA; Teruo KUSAKA; Shigeaki ABE; Mariko NAKAMURA; Yasutaka YAWAKA; Junichiro IIDA; Yasuhiro YOSHIDA; Hidehiko SANO
  Nano Biomedicine, 9, 2, 112, 116, Mar. 2017, [Peer-reviewed]
  English, Scientific journal
• Sandblasting may damage the surface of composite CAD-CAM blocks
  Kumiko Yoshihara; Noriyuki Nagaoka; Yukinori Maruo; Goro Nishigawa; Masao Irie; Yasuhiro Yoshida; Bart Van Meerbeek
  DENTAL MATERIALS, 33, 3, E124, E135, Mar. 2017, [Peer-reviewed]
  English, Scientific journal
• Phosphorylated Pullulan Coating Enhances Titanium Implant Osseointegration in a Pig Model
  Marcio Vivan Cardoso; Amol Chaudhari; Kumiko Yoshihara; Marcelo Ferraz Mesquita; Yasuhiro Yoshida; Bart Van Meerbeek; Katleen Vandamme; Joke Duyck
  INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS, 32, 2, 282, 290, Mar. 2017, [Peer-reviewed]
  English, Scientific journal
• Chemical interaction mechanism of 10-MDP with zirconia
  Noriyuki Nagaoka; Kumiko Yoshihara; Victor Pinheiro Feitosa; Yoshiyuki Tamada; Masao Irie; Yasuhiro Yoshida; Bart Van Meerbeek; Satoshi Hayakawa
  SCIENTIFIC REPORTS, 7, 45563, Mar. 2017, [Peer-reviewed]
  English, Scientific journal
• Limited interaction of a self- adhesive flowable composite with dentin/enamel characterized by TEM
  Atsushi Mine; Jan De Munck; Annelies Van Ende; Andre Poitevin; Mariko Matsumoto; Yasuhiro Yoshida; Takuo Kuboki; Kirsten L. Van Landuyt; Hirofumi Yatani; Bart Van Meerbeek
  DENTAL MATERIALS, 33, 2, 209, 217, Feb. 2017, [Peer-reviewed]
  English, Scientific journal
• Migration and Osteogenic Differentiation of Mesenchymal Stem Cells on Titanium with a Micro-Patterned Gelatin Matrix
  Mariko Kawai; Noriyuki Nagaoka; Masakazu Inubushi; Yoshihiro Ito; Yasuhiro Yoshida; Kiyoshi Ohura
  International Journal of Theoretical and Applied Nanotechnology, Avestia Publishing, 2017
  Scientific journal
• Controlled ion release property of glass ionomer cement containing nanoporous silica particles
  K. Nakanishi; Y. Bando; T. Katsurayama; S. Morimoto; S. Abe; S. Yamagata; Y. Yoshida; J. Iida
  Key Engineering Materials, 720, 17, 20, Trans Tech Publications Ltd, 2017, [Peer-reviewed]
  English, International conference proceedings
• Effect of Micro/Nano-Patterned Surfaces on Cell Adhesion of Ca9-22 cells
  Naoyuki Kaga; Rumi Horiuchi; Atsuro Yokoyama; Tsukasa Akasaka; Yasuhiro Yoshida
  e-Journal of Surface Science and Nanotechnology, 15, 1, 6, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• Charge and controlled release of epigallocatechin gallate by glass ionomer cement containing nanoporous silica particles.
  Nakamura K; Nakanishi K; Bando Y; Hasebe A; Hyono A; Abe S; Shibata K; Yoshida Y; Iida J; Yawaka Y
  Nano Biomedicine, 9, 1, 29, 34, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• Effect of Protein Adsorption on Alignment of Human Gingival Fibroblasts on Grooved Composite Resin
  Akasaka Tsukasa; Imamura Takuya; Miyaji Hirofumi; Kaga Naoyuki; Yokoyama Atsuro; Yoshida Yasuhiro
  E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY, 14, 10 Dec. 2016, [Peer-reviewed]
  English, Scientific journal
• Adhesion of Human Osteoblast-like Cells (Saos-2 cells) on Micro/nanopatterned Structures Sputter-Coated with Titanium
  KAGA Naoyuki; AKASAKA Tsukasa; HORIUCHI Rumi; YOSHIDA Yasuhiro; YOKOYAMA Atsuro
  Nano Biomedicine, 8, 2, 74‐82, Dec. 2016, [Peer-reviewed]
  English
• Adhesion of Osteoblast-like cells (Saos-2) on micro-/submicro-patterned apatite scaffolds fabricated with apatite cement paste by micro-molding
  T Akasaka; H Miyaji; N Kaga; A Yokoyama; S Abe; Y Yoshida
  Nano Biomed, 8, 112, 122, Dec. 2016, [Peer-reviewed]
• Evaluation of Fluorescent Orthodontic Adhesives Containing Y2O3:Eu3+ Particles
  Shuichi YAMAGATA; Yoshiaki SATO; Takaaki YAMAMOTO; Shigeaki ABE; Tsukasa AKASAKA; Yasuhiro YOSHIDA; Junichiro IIDA
  Nano Biomedicine, 8, 1, 35, 40, Oct. 2016, [Peer-reviewed]
  English, Scientific journal
• Evaluation of a Gliding Arc Discharge Non-thermal Atmospheric Pressure Plasma Device for Orthodontic Bonding
  Shuichi YAMAGATA; Takaaki YAMAMOTO; Yoshiaki SATO; Shigeaki ABE; Tsukasa AKASAKA; Yasuhiro YOSHIDA; Junichiro IIDA
  Nano Biomedicine, 8, 1, 57, 63, Oct. 2016, [Peer-reviewed]
  English, Scientific journal
• Effectiveness and stability of silane coupling agent incorporated in 'universal' adhesives
  Kumiko Yoshihara; Noriyuki Nagaoka; Akinari Sonoda; Yukinori Maruo; Yoji Makita; Takumi Okihara; Masao Irie; Yasuhiro Yoshida; Bart Van Meerbeek
  DENTAL MATERIALS, 32, 10, 1218, 1225, Oct. 2016, [Peer-reviewed]
  English, Scientific journal
• Development of Poly(L-lactic acid)/Organically Modified Montmorillonite Nanocomposites for the Fabrication of Orthodontic Anchoring Screws
  Nakanishi, Ko; Yamagata, Shuichi; Akasaka, Tsukasa; Abe, Shigeaki; Yoshida, Yasuhiro; Iida, Junichiro
  Journal of Nanoscience and Nanotechnology, 16, 9, 9292, 9297, Sep. 2016, [Peer-reviewed]
  English, Scientific journal
• Activation of inflammasomes in dendritic cells and macrophages byMycoplasma salivarium
  Sugiyama Masahiro; Saeki Ayumi; Hasebe Akira; Kamesaki Ryousuke; Yoshida Yasuhiro; Kitagawa Yoshimasa; Suzuki Toshihiko; Shibata Ken-ichiro
  Molecular Oral Microbiology, 31, 3, 259, 269, Wiley, Jun. 2016
  English, Interleukin-1β (IL-1β) plays crucial roles in the pathogenesis of periodontal disease.IL-1β is produced after the processing of pro-IL-1β by caspase-1, which is activated bythe inflammasome-a multiprotein complex comprising NLR, the adaptor protein ASC,and procaspase-1. Mycoplasma salivarium preferentially inhabits the gingival sulcusand the incidence and number of organisms in the oral cavity increase significantly withthe progression of periodontal disease. To initially clarify the association of thisorganism with periodontal diseases, this study determined whether it induces IL-1βproduction by innate immune cells such as dendritic cells or macrophages by usingMycoplasma pneumoniae as a positive control. Both live and heat-killed M. salivariumand M. pneumoniae cells induced IL-1β production by XS106 murine dendritic cells aswell as pyroptosis. The activities were significantly downregulated by silencing ofcaspase-1. Bone marrow-derived macrophage (BMMs) from wild-type and NLRP3-,ASC-, and caspase-1–deficient mice were examined for IL-1β production in response tothese mycoplasmas. Live M. salivarium and M. pneumoniae cells almost completelylost the ability to induce IL-1β production by BMMs from ASC- and caspase-1–deficient mice. Their activities toward BMMs from NLRP3-deficient mice weresignificantly but not completely attenuated. These results suggest that live M.salivarium and M. pneumoniae cells can activate several types of inflammasomesincluding the NLRP3 inflammasome. M. salivarium as well as M. pneumoniae cells canactivate THP-1 human monocytic cells to induce IL-1β production.Thus, the present finding that M. salivarium induces IL-1β production by dendriticcells and macrophages may suggest the association of this organism with periodontaldiseases.
• Assessment of Size and Morphological Effects of Ceramics Nanoparticles on Their Biocompatibility
  Satoshi Tsuchiya; Shigeaki Abe; Yuko Era; Yukari Nakagawa; Shuichi Yamagata; Mariko Nakamura; Teruo Kusaka; Yasuhiro Yoshida; Tetsu Yonezawa; Junichiro Iida
  Nanoscience and Nanotechnology Letters, 8, 4, 329, 334, Apr. 2016, [Peer-reviewed]
  English, Scientific journal
• Evaluation of the Biocompatibility of Ceramic Nanoparticles with A549 Lung Epithelial Cells
  Nozomu Kiyama; Shigeaki Abe; Shuichi Yamagata; Yasuhiro Yoshida; Junichiro Iida
  Nanoscience and Nanotechnology Letters, 8, 4, 310, 315, Apr. 2016, [Peer-reviewed]
  English, Scientific journal
• Interference of functional monomers with polymerization efficiency of adhesives
  Masao Hanabusa; Kumiko Yoshihara; Yasuhiro Yoshida; Takumi Okihara; Takatsugu Yamamoto; Yasuko Momoi; Bart Van Meerbeek
  EUROPEAN JOURNAL OF ORAL SCIENCES, 124, 2, 204, 209, Apr. 2016, [Peer-reviewed]
  English, Scientific journal
• Cytocompatibility of HeLa Cells to Nano-Sized Ceramics Particles
  Eri Seitoku; Shigeaki Abe; Teruo Kusaka; Mariko Nakamura; Satoshi Inoue; Yasuhiro Yoshida; Hidehiko Sano
  JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 16, 4, 3373, 3377, Apr. 2016, [Peer-reviewed]
  English, Scientific journal
• Density Functional Theory (DFT) Study on the Ternary Interaction System of the Fluorinated Ethylene Carbonate, Li+ and Graphene Model
  Mami Mutoh; Shigeaki Abe; Teruo Kusaka; Mariko Nakamura; Yasuhiro Yoshida; Junichiro Iida; Hiroto Tachikawa
  ATOMS, 4, 1, Mar. 2016, [Peer-reviewed]
  English, Scientific journal
• Estimation of Biocompatibility of Nano-Sized Ceramic Particles with Osteoblasts, Osteosarcomas and Hepatocytes by Static and Time-Lapse Observation
  Shigeaki Abe; Eri Seitoku; Nobuki Lwadera; Yusuke Hamba; Shuichi Yamagata; Tsukasa Akasaka; Teruo Kusaka; Satoshi Lnoue; Yasutaka Yawaka; Junichiro Lida; Hidehiko Sano; Tetsu Yonezawa; Yasuhiro Yoshida
  JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 12, 3, 472, 480, Mar. 2016, [Peer-reviewed]
  English, Scientific journal
• Effects of surface properties of montmorillonite for their cytocompatibility
  Ko Nakanishi; Shigeaki Abe; Shuichi Yamagata; Yasuhiro Yoshida; Junichiro Iida
  Key Engineering Materials, 696, 80, 84, Trans Tech Publications Ltd, 2016, [Peer-reviewed]
  English, International conference proceedings
• Structural and Chemical Analysis of the Zirconia-Veneering Ceramic Interface
  M. Inokoshi; K. Yoshihara; N. Nagaoka; M. Nakanishi; J. De Munck; S. Minakuchi; K. Vanmeensel; F. Zhang; Y. Yoshida; J. Vleugels; I. Naert; B. Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 95, 1, 102, 109, Jan. 2016, [Peer-reviewed]
  English, Scientific journal
• Functional monomer impurity affects adhesive performance.
  Yoshihara K; Nagaoka N; Okihara T; Kuroboshi M; Hayakawa S; Maruo Y; Nishigawa G; De Munck J; Yoshida Y; Van Meerbeek B
  Dental materials : official publication of the Academy of Dental Materials, 31, 12, 1493, 1501, Dec. 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: The functional monomer 10-MDP has been considered as one of the best performing functional monomers for dental adhesives. Different adhesives containing 10-MDP are commercially available, among which many so-called 'universal' adhesives. We hypothesize that the quality of the functional monomer 10-MDP in terms of purity may affect bonding performance. METHODS: We therefore characterized three different 10-MDP versions (10-MDP_KN provided by Kuraray Noritake; 10-MDP_PCM provided by PCM; 10-MDP_DMI provided by DMI) using NMR, and analyzed their ability to form 10-MDP_Ca salts on dentin using XRD. The 'immediate' and 'aged' micro-tensile bond strength (μTBS) to dentin of three experimental 10-MDP primers was measured. The resultant interfacial adhesive-dentin ultra-structure was characterized using TEM. RESULTS: NMR disclosed impurities and the presence of 10-MDP dimer in 10-MDP_PCM and 10-MDP_DMI. 10-MDP_PCM and 10-MDP_DMI appeared also sensitive to hydrolysis. 10-MDP_KN, on the contrary, contained less impurities and dimer, and did not undergo hydrolysis. XRD revealed more intense 10-MDP_Ca salt deposition on dentin induced by 10-MDP_KN. The adhesive based on the experimental 10-MDP_KN primer resulted in a significantly higher 'immediate' bond strength that remained stable upon aging; the μTBS of the experimental 10-MDP_PCM and 10-MDP_DMI adhesives significantly dropped upon aging. TEM revealed thicker hybridization and more intense nano-layering for 10-MDP_KN. SIGNIFICANCE: It was concluded that primer impurities and the presence of 10-MDP dimer affected not only hybridization, but also reduced the formation of 10-MDP_Ca salts and nano-layering. 10-MDP in a high purity grade is essential to achieve durable bonding.
• Bone engineering by phosphorylated-pullulan and β-TCP composite.
  Takahata T; Okihara T; Yoshida Y; Yoshihara K; Shiozaki Y; Yoshida A; Yamane K; Watanabe N; Yoshimura M; Nakamura M; Irie M; Van Meerbeek B; Tanaka M; Ozaki T; Matsukawa A
  Biomedical materials (Bristol, England), 10, 6, 065009, Nov. 2015, [Peer-reviewed]
• Early Initiation of Endochondral Ossification of Mouse Femur Cultured in Hydrogel with Different Mechanical Stiffness
  Gulsan Ara Sathi; Kodai Kenmizaki; Satoshi Yamaguchi; Hitoshi Nagatsuka; Yasuhiro Yoshida; Aira Matsugaki; Takuya Ishimoto; Satoshi Imazato; Takayoshi Nakano; Takuya Matsumoto
  Tissue Engineering - Part C: Methods, 21, 6, 567, 575, 01 Jun. 2015
  Scientific journal
• Effect of remaining dentin thickness on microtensile bond strength of current adhesive systems
  Shihchun Ting; A. F. M. Almas Chowdhury; Feng Pan; Jiale Fu; Jihow Sun; Shinichi Kakuda; Shuhei Hoshika; Yasuhiro Matsuda; Takatsumi Ikeda; Yasuko Nakaoki; Shigeaki Abe; Yasuhiro Yoshida; Hidehiko Sano
  DENTAL MATERIALS JOURNAL, 34, 2, 181, 188, Apr. 2015
  English, Scientific journal
• Preparation of a poly(lactic acid)/montmorillonite nanocomposite
  NAKANISHI KO; YAMAGATA SHUICHI; AKASAKA TSUKASA; ABE SHIGEAKI; YOSHIDA YASUHIRO; IIDA JUNICHIRO
  Key Eng Mater, 631, 151, 155, 2015
  English
• Simple Size-controlled Preparation of Micro-/nano-sized Fullerene C₆₀ Particles using Different Chain-length Diaminoalkane Compounds
  Mami Mutoh; Shigeaki Abe; Katsutoshi Nakayama; Saori Yoshizawa; Mariko Nakamura; Teruo Kusaka; Yasuhiro Yoshida; Junichiro Iida; Tomoya Takada
  Nano Biomedicine, 2015
  English, Scientific journal
• Electroconductive pretreatment of several types of non-conductive wet biological samples for SEM using a room temperature ionic liquid: simple and rapid conductive preparation
  Mami Mutoh; Shigeaki Abe; Atsushi Hyono; Hiroshi Matsuura; Ko Nakanishi; Koji Kawai; Mariko Nakamura; Satoshi Inoue; Yasuhiro Yoshida; Junichiro Iida; Tetsu Yonezawa
  Nano Biomedicine, 7, 2, 80, 2015, [Peer-reviewed]
• Ultra-structural evaluation of an anodic oxidated titanium dental implant
  Akiyoshi Yamagami; Noriyuki Nagaoka; Kumiko Yoshihara; Mariko Nakamura; Hajime Shirai; Takuya Matsumoto; Kazuomi Suzuki; Yasuhiro Yoshida
  DENTAL MATERIALS JOURNAL, 33, 6, 828, 834, Dec. 2014
  English, Scientific journal
• Dentin-smear remains at self-etch adhesive interface
  Atsushi Mine; Jan De Munck; Marcio Vivan Cardoso; Kirsten L. Van Landuyt; Andre Poitevin; Annelies Van Ende; Mariko Matsumoto; Yasuhiro Yoshida; Talmo Kuboki; Hirofumi Yatani; Bart Van Meerbeek
  DENTAL MATERIALS, 30, 10, 1147, 1153, Oct. 2014
  English, Scientific journal
• A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering
  Tetsuro Mazaki; Yasuyuki Shiozaki; Kentaro Yamane; Aki Yoshida; Mariko Nakamura; Yasuhiro Yoshida; Di Zhou; Takashi Kitajima; Masato Tanaka; Yoshihiro Ito; Toshifumi Ozaki; Akihiro Matsukawa
  SCIENTIFIC REPORTS, 4, Mar. 2014, [Peer-reviewed]
  English, Scientific journal
• Novel Fluoro-carbon Functional Monomer for Dental Bonding
  K. Yoshihara; Y. Yoshida; S. Hayakawa; N. Nagaoka; S. Kamenoue; T. Okihara; T. Ogawa; M. Nakamura; A. Osaka; B. Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 93, 2, 189, 194, Feb. 2014, [Peer-reviewed]
  English, Scientific journal
• Hybrid sponge comprised of galactosylated chitosan and hyaluronic acid mediates the co-culture of hepatocytes and endothelial cells
  Yi Shang; Miho Tamai; Ryusei Ishii; Noriyuki Nagaoka; Yasuhiro Yoshida; Masamichi Ogasawara; Jun Yang; Yoh-ichi Tagawa
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117, 1, 99, 106, Jan. 2014
  English, Scientific journal
• In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells
  Tetsuro Mazaki; Takashi Kitajima; Yasuyuki Shiozaki; Miwa Sato; Megumi Mino; Aki Yoshida; Mariko Nakamura; Yasuhiro Yoshida; Masato Tanaka; Toshifumi Ozaki; Akihiro Matsukawa; Yoshihiro Ito
  Journal of Functional Foods, 6, 1, 241, 247, 2014, [Peer-reviewed]
  English, Scientific journal
• 2P059 Effects of chemical structure of hydrophilic tertaammonium-type ionic liquids for stability of higher-order structure of proteins(01C. Protein: Property,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))
  Abe Shigeaki; Hyono Atasushi; Kaneko Nobuhiro; Kaneko Kotaro; Kawai Koji; Yoshida Yasuhiro
  Seibutsu Butsuri, 54, 1, S204, The Biophysical Society of Japan General Incorporated Association, 2014
  English
• Bone tissue response to implant surfaces functionalized with phosphate-containing polymers
  Marcio Vivan Cardoso; Amol Chaudhari; Yasuhiro Yoshida; Bart Van Meerbeek; Ignace Naert; Joke Duyck
  CLINICAL ORAL IMPLANTS RESEARCH, 25, 1, 91, 100, Jan. 2014, [Peer-reviewed]
  English, Scientific journal
• Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces
  Xi Yang; Liping Zhu; Seiichi Tada; Di Zhou; Takashi Kitajima; Takashi Isoshima; Yasuhiro Yoshida; Mariko Nakamura; Weiqun Yan; Yoshihiro Ito
  INTERNATIONAL JOURNAL OF NANOMEDICINE, 9, 2753, 2765, 2014, [Peer-reviewed]
  English, Scientific journal
• Adhesive interfacial interaction affected by different carbon-chain monomers.
  Yoshihara K; Yoshida Y; Nagaoka N; Hayakawa S; Okihara T; De Munck J; Maruo Y; Nishigawa G; Minagi S; Osaka A; Van Meerbeek B
  Dental materials : official publication of the Academy of Dental Materials, 29, 8, 888, 897, Aug. 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVES: The functional monomer 10-methacryloxydecyl dihydrogen phosphate (10-MDP), recently used in more self-etch adhesives, chemically bonds to hydroxyapatite (HAp) and thus tooth tissue. Although the interfacial interaction of the phosphoric-acid functional group of 10-MDP with HAp-based substrates has well been documented, the effect of the long carbon-chain spacer of 10-MDP on the bonding effectiveness is far from understood. METHODS: We investigated three phosphoric-acid monomers, 2-methacryloyloxyethyl dihydrogen phosphate (2-MEP), 6-methacryloyloxyhexyl dihydrogen phosphate (6-MHP) and 10-MDP, that only differed for the length of the carbon chain, on their chemical interaction potential with HAp and dentin, this correlatively using X-ray diffraction (XRD) and transmission electron microscopy (TEM). Commercial 6-MHP and 10-MDP containing adhesives were investigated as well. RESULTS: XRD revealed that on HAp only 10-MDP produced monomer-calcium salts in the form of 'nano-layering', while on dentin all monomers produced 'nano-layering', but with a varying intensity in the order of 10-MDP>6-MHP>2-MEP. TEM confirmed that 10-MDP formed the thickest hybrid and adhesive layer. XRD and TEM revealed 'nano-layering' for all commercial adhesives on dentin, though less intensively for the 6-MHP containing adhesive than for the 10-MDP ones. SIGNIFICANCE: It is concluded that not only the phosphoric-acid group but also the spacer group, and its length, affect the chemical interaction potential with HAp and dentin. In addition, the relatively strong 'etching' effect of 10-MDP forms more stable monomer-Ca salts, or 'nano-layering', than the two shorter carbon-chain monomers tested, thereby explaining, at least in part, the better bond durability documented with 10-MDP containing adhesives.
• Visible Light-Induced Crosslinkable Gelatin for Direct Pulp Capping
  Mariko Nakamura; Yasuhiro Yoshida; Yoshihiro Ito
  BIOCERAMICS 24, 529-530, 543, +, 2013
  English, International conference proceedings
• Enhanced in vivo osteogenesis by nanocarrier-fused bone morphogenetic protein-4.
  Yasuyuki Shiozaki; Takashi Kitajima; Tetsuro Mazaki; Aki Yoshida; Masato Tanaka; Akihiro Umezawa; Mariko Nakamura; Yasuhiro Yoshida; Yoshihiro Ito; Toshifumi Ozaki; Akihiro Matsukawa
  International journal of nanomedicine, 8, 1349, 60, 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal, PURPOSE: Bone defects and nonunions are major clinical skeletal problems. Growth factors are commonly used to promote bone regeneration; however, the clinical impact is limited because the factors do not last long at a given site. The introduction of tissue engineering aimed to deter the diffusion of these factors is a promising therapeutic strategy. The purpose of the present study was to evaluate the in vivo osteogenic capability of an engineered bone morphogenetic protein-4 (BMP4) fusion protein. METHODS: BMP4 was fused with a nanosized carrier, collagen-binding domain (CBD), derived from fibronectin. The stability of the CBD-BMP4 fusion protein was examined in vitro and in vivo. Osteogenic effects of CBD-BMP4 were evaluated by computer tomography after intramedullary injection without a collagen-sponge scaffold. Recombinant BMP-4, CBD, or vehicle were used as controls. Expressions of bone-related genes and growth factors were compared among the groups. Osteogenesis induced by CBD-BMP4, BMP4, and CBD was also assessed in a bone-defect model. RESULTS: In vitro, CBD-BMP4 was retained in a collagen gel for at least 7 days while BMP4 alone was released within 3 hours. In vivo, CBD-BMP4 remained at the given site for at least 2 weeks, both with or without a collagen-sponge scaffold, while BMP4 disappeared from the site within 3 days after injection. CBD-BMP4 induced better bone formation than BMP4 did alone, CBD alone, and vehicle after the intramedullary injection into the mouse femur. Bone-related genes and growth factors were expressed at higher levels in CBD-BMP4-treated mice than in all other groups, including BMP4-treated mice. Finally, CBD-BMP4 potentiated more bone formation than did controls, including BMP4 alone, when applied to cranial bone defects without a collagen scaffold. CONCLUSION: Altogether, nanocarrier-CBD enhanced the retention of BMP4 in the bone, thereby promoting augmented osteogenic responses in the absence of a scaffold. These results suggest that CBD-BMP4 may be clinically useful in facilitating bone formation.
• Phosphorylated Pullulan Bioadhesive for Regeneration and Reconstruction of Bone and Tooth
  Yoshida Yasuhiro; Okihara Takumi; Nakamura Mariko; Matsumoto Takuya
  BIOCERAMICS 24, 529-530, 516, +, 2013, [Peer-reviewed]
• Chemical analyses in dental adhesive technology
  Yasuhiro Yoshida; Satoshi Inoue
  Japanese Dental Science Review, 48, 2, 141, 152, Aug. 2012
  English
• Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis
  Kenichi Harimoto; Yasuhiro Yoshida; Kumiko Yoshihara; Noriyuki Nagaoka; Takuya Matsumoto; Yoh-ichi Tagawa
  DENTAL MATERIALS JOURNAL, 31, 4, 674, 680, Jul. 2012
  English, Scientific journal
• X-ray diffraction analysis of three-dimensional self-reinforcing monomer and its chemical interaction with tooth and hydroxyapatite
  Yasuhiro Yoshida; Kumiko Yoshihara; Noriyuki Nagaoka; Masao Hanabusa; Takuya Matsumoto; Yasuko Momoi
  DENTAL MATERIALS JOURNAL, 31, 4, 697, 702, Jul. 2012
  English, Scientific journal
• Preparation of low-crystalline apatite nanoparticles and their coating onto quartz substrates
  Masakazu Kawashita; Koji Taninai; Zhixia Li; Kunio Ishikawa; Yasuhiro Yoshida
  JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 23, 6, 1355, 1362, Jun. 2012
  English, Scientific journal
• Effect of low-shrinking composite on the bonding effectiveness of two adhesives in occlusal Class-I cavities
  Atsushi Mine; Jan De Munck; Marcio V. Cardoso; Kirsten L. Van Landuyt; Andre Poitevin; Takuo Kuboki; Yasuhiro Yoshida; Kazuomi Suzuki; Bart Van Meerbeek
  DENTAL MATERIALS JOURNAL, 31, 3, 418, 426, May 2012
  English, Scientific journal
• Effects of functional monomers and photo-initiators on the degree of conversion of a dental adhesive.
  Oguri M; Yoshida Y; Yoshihara K; Miyauchi T; Nakamura Y; Shimoda S; Hanabusa M; Momoi Y; Van Meerbeek B
  Acta biomaterialia, 8, 5, 1928, 1934, May 2012, [Peer-reviewed]
• Self-assembled Nano-layering at the Adhesive interface.
  Yoshida Y; Yoshihara K; Nagaoka N; Hayakawa S; Torii Y; Ogawa T; Osaka A; Meerbeek BV
  Journal of Dental Research, 91, 4, 376, 381, Apr. 2012, [Peer-reviewed]
  English, Scientific journal
• Real time assessment of surface interactions with a titanium passivation layer by surface plasmon resonance
  Hirata, Isao; Yoshida, Yasuhiro; Nagaoka, Noriyuki; Hiasa, Kyou; Abe, Yasuhiko; Maekawa, Kenji; Kuboki, Takuo; Akagawa, Yasumasa; Suzuki, Kazuomi; Van Meerbeek; Bart
  ACTA BIOMATERIALIA, 8, 3, 1260, 1266, ELSEVIER SCI LTD, Mar. 2012, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Due to the high corrosion resistance and strength to density ratio titanium is widely used in industry, and also in a gamut of medical applications. Here we report for the first time on our development of a titanium passivation layer sensor that makes use of surface plasmon resonance (SPR). The deposited titanium metal layer on the sensor was passivated in air, similarly to titanium medical devices. Our Ti-SPR sensor" enables analysis of biomolecule interactions with the passivated surface of titanium in real time. As a proof of concept, corrosion of a titanium passivation layer exposed to acid was monitored in real time. The Ti-SPR sensor can also accurately measure the time-dependence of protein adsorption onto the titanium passivation layer at sub-nanogram per square millimeter accuracy. Besides such SPR analyses, SPR imaging (SPRI) enables real time assessment of chemical surface processes that occur simultaneously at "multiple independent spots" on the Ti-SPR sensor, such as acid corrosion or adhesion of cells. Our Ti-SPR sensor will therefore be very useful to study titanium corrosion phenomena and biomolecular titanium-surface interactions with application in
• Time-Resolved Switching Characteristic in Magnetic Tunnel Junction with Spin Transfer Torque Write Scheme
  Fumitaka Iga; Yasuhiro Yoshida; Shoji Ikeda; Takahiro Hanyu; Hideo Ohno; Tetsuo Endoh
  JAPANESE JOURNAL OF APPLIED PHYSICS, 51, 2, 02BM02.1-02BM02.5, Feb. 2012
  English, Scientific journal
• BMP-2 gene transfer under various conditions with in vivo electroporation and bone induction
  Hiromitsu Yamamoto; Mariko Kawai; Noriko Shiotsu; Minori Watanabe; Yasuhiro Yoshida; Kazuomi Suzuki; Hiroki Maruyama; Jun-Ichi Miyazaki; Mika Ikegame; Kazuhisa Bessho; Toshio Yamamoto
  Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 24, 1, 49, 53, Elsevier Ltd, 2012, [Peer-reviewed]
  English, Scientific journal
• HEMA inhibits interfacial nano-layering of the functional monomer MDP.
  Yoshida Y; Yoshihara K; Hayakawa S; Nagaoka N; Okihara T; Matsumoto T; Minagi S; Osaka A; Van Landuyt K; Van Meerbeek B
  J Dent Res., 91, 1060, 1065, 2012
• Nanolayering of phosphoric acid ester monomer on enamel and dentin.
  Yoshihara K; Yoshida Y; Hayakawa S; Nagaoka N; Irie M; Ogawa T; Van Landuyt KL; Osaka A; Suzuki K; Minagi S; Van Meerbeek B
  Acta biomaterialia, 7, 8, 3187, 3195, Aug. 2011, [Peer-reviewed]
  English, Scientific journal
• Sox9 expression during fracture repair
  Yuko Shintaku; Takashi Murakami; Takeshi Yanagita; Noriaki Kawanabe; Tomohiro Fukunaga; Kiyomi Matsuzaki; Setsuko Uematsu; Yasuhiro Yoshida; Hiroshi Kamioka; Teruko Takano-Yamamoto; Kenji Takada; Takashi Yamashiro
  Cells Tissues Organs, 194, 1, 38, 48, Jun. 2011, [Peer-reviewed]
  English, Scientific journal
• Hydrolytic stability of one-step self-etching adhesives bonded to dentin.
  Fukuoka A; Koshiro K; Inoue S; Yoshida Y; Tanaka T; Ikeda T; Suzuki K; Sano H; Meerbeek BV
  The journal of adhesive dentistry, 13, 3, 243, 248, 3, Jun. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal, PURPOSE: To evaluate the hydrolytic stability of three one-step self-etching adhesives (1-SEAs) bonded to dentin through bond strength testing and ultra-morphological interfacial analysis before and after long-term thermocycling. MATERIALS AND METHODS: Eighteen flattened mid-coronal dentin surfaces of extracted human molars were subjected to bonding treatment using three 1-SEAs (Clearfil S3 Bond, Kuraray (S3), G-Bond, GC (GB), Absolute, Dentsply-Sankin (AB)), after which the bonded surfaces were built up with composite. After storage overnight at 37°C, the specimens were sectioned into slabs and further trimmed into an hourglass shape with an interface area of approximately 1 mm2. The specimens were left untouched (control) or were thermocycled for 100,000 cycles. The microtensile bond strength (μTBS) was measured and the ultrastructure of the adhesive/dentin interface characterized using transmission electron microscopy (TEM). RESULTS: Long-term thermocycling significantly decreased the μTBS of all one-step adhesives tested (p < 0.05, one way ANOVA and Games-Howell test). TEM revealed a similar interfacial ultrastructure before and after thermocycling for S3. For GB, many voids were observed at the interface after 100,000 thermocycles. Regarding AB, collagen fibrils could no longer be clearly observed upon staining, while the adjacent unaffected dentin was rich in voids. CONCLUSION: The bond strength and ultramorphological data demonstrated that the bond of 1-SEAs to dentin degrades with time, although the degree of degradation is obviously material dependent.
• Self-etch monomer-calcium salt deposition on dentin.
  Yoshihara K; Yoshida Y; Hayakawa S; Nagaoka N; Torii Y; Osaka A; Suzuki K; Minagi S; Van Meerbeek B; Van Landuyt KL
  J Dent Res., 90, 5, 602, 606, May 2011, [Peer-reviewed]
  English
• Detection of synthetic RGDS(PO3H2)PA peptide adsorption using a titanium surface plasmon resonance biosensor
  Yasuhiko Abe; Kyou Hiasa; Isao Hirata; Yohei Okazaki; Keisuke Nogami; Wataru Mizumachi; Yasuhiro Yoshida; Kazuomi Suzuki; Masayuki Okazaki; Yasumasa Akagawa
  JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 22, 3, 657, 661, Mar. 2011, [Peer-reviewed]
  English, Scientific journal
• Effect of functional monomers in all-in-one adhesive systems on formation of enamel/dentin acid-base resistant zone
  Toru Nikaido; Chiaki Ichikawa; Na Li; Tomohiro Takagaki; Alireza Sadr; Yasuhiro Yoshida; Kazuomi Suzuki; Junji Tagami
  Dental Materials Journal, 30, 5, 576, 582, 2011, [Peer-reviewed]
  English, Scientific journal
• State of the art of self-etch adhesives
  B. Van Meerbeek; K. Yoshihara; Y. Yoshida; A. Mine; J. De Munck; K. L. Van Landuyt
  DENTAL MATERIALS, 27, 1, 17, 28, Jan. 2011, [Peer-reviewed]
  English, Scientific journal
• Degree of immobilization of synthetic RGDS(PO3H2)PA peptides on titanium surfaces
  Yasuhiko Abe; Yohei Okazaki; Kyou Hiasa; Isao Hirata; Yasuhiro Yoshida; Tsuyoshi Taji; Kazuomi Suzuki; Masayuki Okazaki; Yasumasa Akagawa
  DENTAL MATERIALS JOURNAL, 29, 6, 668, 672, Nov. 2010, [Peer-reviewed]
  English, Scientific journal
• Visible light-induced crosslinkable gelatin
  Tae Il Son; Makoto Sakuragi; Sawa Takahashi; Sei Obuse; Jeonghwa Kang; Masako Fujishiro; Haruhiko Matsushita; Jiansheng Gong; Shigeru Shimizu; Yusuke Tajima; Yasuhiro Yoshida; Kazuomi Suzuki; Toshio Yamamoto; Mariko Nakamura; Yoshihiro Ito
  ACTA BIOMATERIALIA, 6, 10, 4005, 4010, Oct. 2010, [Peer-reviewed]
  English, Scientific journal
• Nano-controlled molecular interaction at adhesive interfaces for hard tissue reconstruction.
  Yoshihara K; Yoshida Y; Nagaoka N; Fukegawa D; Hayakawa S; Mine A; Nakamura M; Minagi S; Osaka A; Suzuki K; Van Meerbeek B
  Acta biomaterialia, 6, 9, 3573, 3582, Sep. 2010, [Peer-reviewed]
  English, Scientific journal
• Antibacterial effect of bactericide immobilized in resin matrix.
  Namba N; Yoshida Y; Nagaoka N; Takashima S; Matsuura-Yoshimoto K; Maeda H; Van Meerbeek B; Suzuki K; Takashiba S
  Dental materials : official publication of the Academy of Dental Materials, 25, 4, 424, 430, 4, Apr. 2009, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: Biomaterials with anti-microbial properties are highly desirable in the oral cavity. Ideally, bactericidal molecules should be immobilized within the biomaterial to avoid unwanted side-effects against surrounding tissues. They may then however loose much of their antibacterial efficiency. The aim of this study was to investigate how much antibacterial effect an immobilized bactericidal molecule still has against oral bacteria. METHODS: Experimental resins containing 0, 1 and 3% cetylpyridinium chloride (CPC) were polymerized, and the bacteriostatic and bactericidal effects against Streptococcus mutans were determined. Adherent S. mutans on HAp was quantitatively determined using FE-SEM and living cells of S. mutans were quantified using real-time RT-PCR. The amount of CPC released from the 0%-, 1%- and 3%-CPC resin sample into water was spectrometrically quantified using a UV-vis recording spectrophotometer. RESULTS: UV spectrometry revealed that less than 0.11 ppm of CPC was released from the resin into water for all specimens, which is lower than the minimal concentration generally needed to inhibit biofilm formation. Growth of S. mutans was significantly inhibited on the surface of the 3%-CPC-containing resin coating, although no inhibitory effect was observed on bacteria that were not in contact with its surface. When immersed in water, the antibacterial capability of 3%-CPC resin lasted for 7 days, as compared to resin that did not contain CPC. SIGNIFICANCE: These results demonstrated that the bactericidal molecule still possessed significant contact bacteriostatic activity when it was immobilized in the resin matrix.
• Ultrasonic cleaning of silica-coated zirconia influences bond strength between zirconia and resin luting material
  Goro Nishigawa; Yukinori Maruo; Masao Irie; Morihiko Oka; Kumiko Yoshihara; Shogo Minagi; Noriyuki Nagaoka; Yasuhiro Yoshida; Kazuomi Suzuki
  DENTAL MATERIALS JOURNAL, 27, 6, 842, 848, Nov. 2008
  English, Scientific journal
• Bonding effectiveness and interfacial characterization of a HEMA/TEGDMA-free three-step etch&rinse adhesive
  Atsushi Mine; Jan De Munck; Kirsten L. Van Landuyt; Andre Poitevin; Takuo Kuboki; Yasuhiro Yoshida; Kazuomi Suzuki; Paul Lambrechts; Bart Van Meerbeek
  JOURNAL OF DENTISTRY, 36, 10, 767, 773, Oct. 2008
  English, Scientific journal
• Influence of the chemical structure of functional monomers on their adhesive performance
  K. L. Van Landuyt; Y. Yoshida; I. Hirata; J. Snauwaert; J. De Munck; M. Okazaki; K. Suzuki; P. Lambrechts; B. Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 87, 8, 757, 761, Aug. 2008
  English, Scientific journal
• Effect of polyphosphoric acid pre-treatment of titanium on attachment, proliferation, and differentiation of osteoblast-like cells (MC3T3-E1)
  Kenji Maekawa; Yasuhiro Yoshida; Atsushi Mine; Bart van Meerbeek; Kazuomi Suzuki; Takuo Kuboki
  CLINICAL ORAL IMPLANTS RESEARCH, 19, 3, 320, 325, Mar. 2008
  English, Scientific journal
• Promotion of hydroxyapatite-associated, stem cell-based bone regeneration by CCN2
  Mitsuaki Ono; Satoshi Kubota; Takuo Fujisawa; Wataru Sonoyama; Harumi Kawakij; Kentaro Akiyama; Kengo Shimono; Masarnitsu Oshima; Takashi Nishida; Yasuhiro Yoshida; Kazuomi Suzuki; Masaharu Takigawa; Takuo Kuboki
  CELL TRANSPLANTATION, 17, 1-2, 231, 240, 2008
  English, Scientific journal
• Dental adhesives and adhesive performance
  Bart Van Meerbeek; Jan De Munck; Kirsten L. Van Landuyt; Atsushi Mine; Paul Lambrechts; Mouhamed Sarr; Mouhamed Sarr; Yasuhiro Yoshida; Kazuomi Suzuki
  Dental Biomaterials: Imaging, Testing and Modelling, 81, 111, Elsevier Inc., 2008, [Peer-reviewed]
  English, In book
• Modification of the titan surface with photoreactive gelatin to regulate cell attachment
  Mojgan Heydari; Hirokazu Hasuda; Makoto Sakuragi; Yasuhiro Yoshida; Kazuorni Suzuki; Yoshihiro Ito
  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 83A, 4, 906, 914, Dec. 2007
  English, Scientific journal
• Systematic review of the chemical composition of contemporary dental adhesives
  Kirsten L. Van Landuyt; Johan Snauwaert; Jan De Munck; Marleen Peurnans; Yasuhiro Yoshida; Andr Poitevin; Eduardo Coutinho; Kazuomi Suzuki; Paul Lambrechtsa; Bart Van Meerbeek
  BIOMATERIALS, 28, 26, 3757, 3785, Sep. 2007
  English
• Origin of interfacial droplets with one-step adhesives
  K. L. Van Landuyt; J. Snauwaert; J. De Munck; E. Coutinho; A. Poitevin; Y. Yoshida; K. Suzuki; P. Lambrechts; B. Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 86, 8, 739, 744, Aug. 2007
  English, Scientific journal
• Gel phase formation at resin-modified glass-ionomer/tooth interfaces
  E. Coutinho; Y. Yoshida; S. Inoue; R. Fukuda; J. Snauwaert; Y. Nakayama; J. De Munck; P. Lambrechts; K. Suzuki; B. Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 86, 7, 656, 661, Jul. 2007
  English, Scientific journal
• Chemical interaction of polyphosphoric acid with titanium and its effect on human bone marrow derived mesenchymal stem cell behavior
  Kenji Maekawa; Yasuhiro Yoshida; Atsushi Mine; Takuo Fujisawa; Bart Van Meerbeek; Kazuomi Suzuki; Takuo Kuboki
  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 82A, 1, 195, 200, Jul. 2007
  English, Scientific journal
• Restoring cervical lesions with flexible composites
  M. Peumans; J. De Munck; K. L. Van Landuyt; P. Kanumilli; Y. Yoshida; S. Inoue; P. Lambrechts; B. Van Meerbeek
  DENTAL MATERIALS, 23, 6, 749, 754, Jun. 2007
  English, Scientific journal
• Promotion of attachment of human bone marrow stromal cells by CCN2
  Mitsuaki Ono; Satoshi Kubota; Takuo Fujisawa; Wataru Sonoyama; Harumi Kawaki; Kentaro Akiyama; Masamitsu Oshima; Takashi Nishida; Yasuhlro Yoshida; Kazuomi Suzuki; Masaharu Takigawa; Takuo Kuboki
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 357, 1, 20, 25, May 2007
  English, Scientific journal
• Chemical interaction between titanium implant surface and amino acids
  Kyou Hiasa; Yasuhiko Abe; Yasuhiro Yoshida; Tsuyoshi Taji; Kazuomi Suzuki; Yasumasa Akagawa
  DENTAL MATERIALS JOURNAL, 26, 2, 156, 161, Mar. 2007
  English, Scientific journal
• Analysis of chemical interaction of 4-MET with hydroxyapatite using XPS
  Nagakane, Kouji; Yoshida, Yasuhiro; Hirata, Isao; Fukuda, Ryuichi; Nakayama, Youichi; Shirai, Kenichi; Ogawa, Tatsuyuki; Suzuki, Kazuomi; Van Meerbeek, Bart; Okazaki, Masayuki
  DENTAL MATERIALS JOURNAL, 25, 4, 645, 649, JAPANESE SOC DENTAL MATERIALS DEVICES, Dec. 2006, [Peer-reviewed]
  English, Scientific journal, Each dental adhesive contains a specific functional monomer that determines its actual adhesive performance to tooth tissue. 4-methacryloxyethyl trimellitic acid (4-MET) is well-known as one of the functional monomers mostly available and consequently widely used in commercial adhesives. We therefore characterized the chemical - interaction of 4-MET with hydroxy-apatite (HAp) using X-ray Photoelectron Spectroscopy (XPS). XPS revealed that the peak representing -COO- of 4-MET shifted to a lower binding energy, when 4-MET was adsorbed onto HAp. Deconvolution of this shifted peak disclosed two components with a peak representing unreacted carboxyl groups and ester groups, and a peak suggesting chemical bonding of other carboxyl groups to Ca of HAp. XPS spectra of HAp treated with 4-MET also disclosed the surface to be enriched in calcium and decreased in phosphorus, indicating that phosphorus was extracted at a, relatively higher rate than calcium. It can thus be concluded that true chemical bonding of 4-MET with calcium present in HAp occurred, as it was proven using XPS.
• Chemical interaction of phosphoric acid ester with hydroxyapatite
  D. Fukegawa; S. Hayakawa; Y. Yoshida; K. Suzuki; A. Osaka; B. Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 85, 10, 941, 944, Oct. 2006
  English, Scientific journal
• Effect of water storage on the bonding effectiveness of 6 adhesives to class I cavity dentin
  J. De Munck; K. Shirai; Y. Yoshida; S. Inoue; K. L. Van Landuyt; P. Lambrechts; K. Suzuki; H. Shintani; B. Van Meerbeek
  OPERATIVE DENTISTRY, 31, 4, 456, 465, Jul. 2006
  English, Scientific journal
• 骨芽細胞様細胞(MC3T3E1)分化に対するチタンの影響
  秋山 謙太郎; 藤澤 拓生; 明貝 文夫; 大野 充明; 吉田 靖弘; 高柴 正悟; 鈴木 一臣; 窪木 拓男
  日本骨代謝学会学術集会プログラム抄録集, 24回, 229, 229, (一社)日本骨代謝学会, Jul. 2006
  Japanese
• Spectroscopic characterization of enamel surfaces irradiated with Er : YAG laser
  Atsushi Mine; Yasuhiro Yoshida; Kazuomi Suzuki; Youichi Nakayama; Hirofumi Yatani; Takuo Kuboki
  DENTAL MATERIALS JOURNAL, 25, 2, 214, 218, Jun. 2006
  English, Scientific journal
• Effect of surface pre-treatment on durability of resin-based cements bonded to titanium
  Y Tsuchimoto; Y Yoshida; M Takeuchi; A Mine; H Yatani; Y Tagawa; B Van Meerbeek; K Suzuki; T Kuboki
  DENTAL MATERIALS, 22, 6, 545, 552, Jun. 2006
  English, Scientific journal
• Development of a self-etch adhesive for resin-modified glass ionomers
  E Coutinho; K Van Landuyt; J De Munck; A Poitevin; Y Yoshida; S Inoue; M Peumans; K Suzuki; P Lambrechts; B Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 85, 4, 349, 353, Apr. 2006
  English, Scientific journal
• Effect of 4-MET- and 10-MDP-based primers on resin bonding to titanium
  Y Tsuchimoto; Y Yoshida; A Mine; M Nakamura; N Nishiyama; B Van Meerbeek; K Suzuki; T Kuboki
  DENTAL MATERIALS JOURNAL, 25, 1, 120, 124, Mar. 2006
  English, Scientific journal
• Analysis of Chemical Interaction of 4-MET with Hydroxyapatite Using XPS :
  NAGAKANE Kouji; YOSHIDA Yasuhiro; HIRATA Isao; FUKUDA Ryuichi; NAKAYAMA Youichi; SHIRAI Kenichi; OGAWA Tatsuyuki; SUZUKI Kazuomi; VAN MEERBEEK Bart; OKAZAKI Masayuki
  Dental materials journal, 25, 4, 645, 649, Japanese Society for Dental Materials and Devices, 2006
  English, Each dental adhesive contains a specific functional monomer that determines its actual adhesive performance to tooth tissue. 4-methacryloxyethyl trimellitic acid (4-MET) is well-known as one of the functional monomers mostly available and consequently widely used in commercial adhesives. We therefore characterized the chemical interaction of 4-MET with hydroxyapatite (HAp) using X-ray Photoelectron Spectroscopy (XPS). XPS revealed that the peak representing -COO- of 4-MET shifted to a lower binding energy, when 4-MET was adsorbed onto HAp. Deconvolution of this shifted peak disclosed two components with a peak representing unreacted carboxyl groups and ester groups, and a peak suggesting chemical bonding of other carboxyl groups to Ca of HAp. XPS spectra of HAp treated with 4-MET also disclosed the surface to be enriched in calcium and decreased in phosphorus, indicating that phosphorus was extracted at a relatively higher rate than calcium. It can thus be concluded that true chemical bonding of 4-MET with calcium present in HAp occurred, as it was proven using XPS.
• New surface modification of titanium implant with phospho-amino acid
  Y Abe; K Hiasa; M Takeuchi; Y Yoshida; K Suzuki; Y Akagawa
  DENTAL MATERIALS JOURNAL, 24, 4, 536, 540, Dec. 2005
  English, Scientific journal
• Hydrolytic stability of self-etch adhesives bonded to dentin
  S Inoue; K Koshiro; Y Yoshida; J De Munck; K Nagakane; K Suzuki; H Sano; B Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 84, 12, 1160, 1164, Dec. 2005
  English, Scientific journal
• Adsorption of polyphosphoric acid to titanium surface and its effect on hBMSC attachment
  K. Maekawa; A. Mine; T. Fujisawa; Y. Yoshida; K. Suzuki; T. Kuboki
  International Congress Series, 1284, 332, 333, Sep. 2005, [Peer-reviewed]
• Micro-rotary fatigue of tooth-biomaterial interfaces
  J De Munck; M Braem; M Wevers; Y Yoshida; S Inoue; K Suzuki; P Lambrechts; B Van Meerbeek
  BIOMATERIALS, 26, 10, 1145, 1153, Apr. 2005
  English, Scientific journal
• Effect of cavity configuration and aging on the bonding effectiveness of six adhesives to dentin
  K Shirai; J De Munck; Y Yoshida; S Inoue; P Lambrechts; K Suzuki; H Shintani; B Van Meerbeek
  DENTAL MATERIALS, 21, 2, 110, 124, Feb. 2005
  English, Scientific journal
• Monomer-solvent phase separation in one-step self-etch adhesives
  KL Van Landuyt; J De Munck; J Snauwaert; E Coutinho; A Poitevin; Y Yoshida; S Inoue; M Peumans; K Suzuki; P Lambrechts; B Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 84, 2, 183, 188, Feb. 2005
  English, Scientific journal
• Effect of conditioner on bond strength of glass-ionomer adhesive to dentin/enamel with and without smear layer interposition
  S Inoue; Y Abe; Y Yoshida; J De Munck; H Sano; K Suzuki; P Lambrechts; B Van Meerbeek
  OPERATIVE DENTISTRY, 29, 6, 685, 692, Nov. 2004
  English, Scientific journal
• Development of self-setting calcium hydroxide preparation using dicalcium phosphate anhydrous and sodium hydrogen phosphate aqueous solution
  M Nakamura; K Ishikawa; Y Yoshida; K Suzuki; T Yamamoto
  JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 112, 1308, 434, 439, Aug. 2004
  English, Scientific journal
• Effect of adding spherical silica filler on physico-mechanical properties of resin modified glass-ionomer cement
  R Tjandrawinata; M Irie; Y Yoshida; K Suzuki
  DENTAL MATERIALS JOURNAL, 23, 2, 146, 154, Jun. 2004
  English, Scientific journal
• Characterization of CO(3)Ap-collagen sponges using x-ray high-resolution microtomography
  M Itoh; A Shimazu; Hirata, I; Y Yoshida; H Shintani; M Okazaki
  BIOMATERIALS, 25, 13, 2577, 2583, Jun. 2004
  English, Scientific journal
• Comparative study on adhesive performance of functional monomers
  Y Yoshida; K Nagakane; R Fukuda; Y Nakayama; M Okazaki; H Shintani; S Inoue; Y Tagawa; K Suzuki; J De Munck; B Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 83, 6, 454, 458, Jun. 2004
  English, Scientific journal
• Four-year water degradation of a resin-modified glass-ionomer adhesive bonded to dentin
  J De Munck; B Van Meerbeek; Y Yoshida; S Inoue; K Suzuki; P Lambrechts
  EUROPEAN JOURNAL OF ORAL SCIENCES, 112, 1, 73, 83, Feb. 2004
  English, Scientific journal
• Development of self-setting calcium hydroxide preparation using dicalcium phosphate anhydrous and sodium hydrogen phosphate aqueous solution
  Mariko Nakamura; Kunio Ishikawa; Yasuhiro Yoshida; Kazuomi Suzuki; Toshio Yamamoto
  Journal of the Ceramic Society of Japan, 112, 1308, 434, 439, Ceramic Society of Japan, 2004, [Peer-reviewed]
  English
• Microtensile bond strengths of one- and two-step self-etch adhesives to bur-cut enamel and dentin
  J De Munck; B Van Meerbeek; Satoshi, I; M Vargas; Y Yoshida; S Armstrong; P Lambrechts; G Vanherle
  AMERICAN JOURNAL OF DENTISTRY, 16, 6, 414, 420, Dec. 2003
  English, Scientific journal
• Action of FGMgCO(3)Ap-collagen composite in promoting bone formation
  Y Yamasaki; Y Yoshida; M Okazaki; A Shimazu; T Kubo; Y Akagawa; T Uchida
  BIOMATERIALS, 24, 27, 4913, 4920, Dec. 2003
  English, Scientific journal
• Microtensile bond strength of eleven contemporary adhesives to enamel
  S Inoue; MA Vargas; Y Abe; Y Yoshida; P Lambrechts; G Vanherle; H Sano; B Van Meerbeek
  AMERICAN JOURNAL OF DENTISTRY, 16, 5, 329, 334, Oct. 2003
  English, Scientific journal
• Bonding efficacy of polyalkenoic acids to hydroxyapatite, enamel and dentin
  R Fukuda; Y Yoshida; Y Nakayama; M Okazaki; S Inoue; H Sano; K Suzuki; H Shintani; B Van Meerbeek
  BIOMATERIALS, 24, 11, 1861, 1867, May 2003
  English, Scientific journal
• Acid pretreatment of titanium implants
  M Takeuchi; Y Abe; Y Yoshida; Y Nakayama; M Okazaki; Y Akagawa
  BIOMATERIALS, 24, 10, 1821, 1827, May 2003
  English, Scientific journal
• Four-year water degradation of total-etch adhesives bonded to dentin
  J De Munck; B Van Meerbeek; Y Yoshida; S Inoue; M Vargas; K Suzuki; P Lambrechts; G Vanherle
  JOURNAL OF DENTAL RESEARCH, 82, 2, 136, 140, Feb. 2003
  English, Scientific journal
• Effect of presilanization filler decontamination on aesthetics and degradation resistance of resin composites
  Y Yoshida; K Shirai; H Shintani; M Okazaki; K Suzuki; B Van Meerbeek
  DENTAL MATERIALS JOURNAL, 21, 4, 383, 395, Dec. 2002
  English, Scientific journal
• Direct adhesive pulp capping: Pulpal healing and ultra-morphology of the resin-pulp interface
  M Fujitani; S Shibata; B Van Meerbeek; Y Yoshida; H Shintani
  AMERICAN JOURNAL OF DENTISTRY, 15, 6, 395, 402, Dec. 2002
  English, Scientific journal
• Synthesis of functionally graded MgCO3 apatite accelerating osteoblast adhesion
  Y Yamasaki; Y Yoshida; M Okazaki; A Shimazu; T Uchida; T Kubo; Y Akagawa; Y Hamada; J Takahashi; N Matsuura
  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 62, 1, 99, 105, Oct. 2002
  English, Scientific journal
• Improved filler-matrix coupling in resin composites
  Y Yoshida; K Shirai; Y Nakayama; M Itoh; M Okazaki; H Shintani; S Inoue; P Lambrechts; G Vanherle; B Van Meerbeek
  JOURNAL OF DENTAL RESEARCH, 81, 4, 270, 273, Apr. 2002
  English, Scientific journal
• Thermal analysis of dental resins cured with blue light-emitting diodes (LEDs)
  Y Nomura; W Teshima; N Tanaka; Y Yoshida; Y Nahara; M Okazaki
  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 63, 2, 209, 213, Apr. 2002
  English, Scientific journal
• Adhesion/decalcification mechanisms of acid interactions with human hard tissues
  M Yoshioka; Y Yoshida; S Inoue; P Lambrechts; G Vanherle; Y Nomura; M Okazaki; H Shintani; B Van Meerbeek
  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 59, 1, 56, 62, Jan. 2002
  English, Scientific journal
• Chemical interaction of acids with apatitic substrates
  Y Yoshida; M Okazaki; B Van Meerbeek
  TISSUE ENGINEERING FOR THERAPEUTIC USE 6, 1243, 161, 168, 2002, [Peer-reviewed]
  English, International conference proceedings
• 骨芽細胞の接着特性ならびに石灰化能に及ぼすMg2+の影響
  岡崎 正之; 山崎 保彦; 吉田 靖弘; 島津 篤
  マグネシウム, 20, 1, 46, 46, (一社)日本マグネシウム学会, Nov. 2001
  Japanese
• 傾斜機能性Mg含有炭酸アパタイト・コラーゲン複合体の細胞ならびに組織との親和性
  山崎 保彦; 吉田 靖弘; 島津 篤; 岡崎 正之
  マグネシウム, 19, 1～2, 48, 48, (一社)日本マグネシウム学会, Oct. 2001
  Japanese
• Effect of remaining dentin thickness and the use of conditioner on microtensile bond strength of a glass-ionomer adhesive
  S Inoue; B Van Meerbeek; Y Abe; Y Yoshida; P Lambrechts; G Vanherle; H Sano
  DENTAL MATERIALS, 17, 5, 445, 455, Sep. 2001
  English, Scientific journal
• Affinity binding phenomena of DNA onto apatite crystals
  M Okazaki; Y Yoshida; S Yamaguchi; M Kaneno; JC Elliott
  BIOMATERIALS, 22, 18, 2459, 2464, Sep. 2001
  English, Scientific journal
• Adhesives and cements to promote preservation dentistry
  B Van Meerbeek; M Vargas; S Inoue; Y Yoshida; M Peumans; P Lambrechts; G Vanherle
  OPERATIVE DENTISTRY, 119, 144, Aug. 2001
  English, Scientific journal
• Adhesion to and decalcification of hydroxyapatite by carboxylic acids
  Y Yoshida; B Van Meerbeek; Y Nakayama; M Yoshioka; J Snauwaert; Y Abe; P Lambrechts; G Vanherle; M Okazaki
  JOURNAL OF DENTAL RESEARCH, 80, 6, 1565, 1569, Jun. 2001
  English, Scientific journal
• A new concept of CO3 apatite-collagen composites with adhesion motif.
  M Okazaki; Y Yamazaki; Y Yoshida; A Shimadzu; Y Hamada; J Takahashi
  JOURNAL OF DENTAL RESEARCH, 80, 4, 1337, 1337, Apr. 2001
  English
• Micro-tensile bond strength of eleven modern adhesives to dentin
  Inoue, S; Vargas, M.A; Abe, Y; Yoshida, Y; Lambrechts, P; Vanherle, G; Sano, H; Van Meerbeek, B
  Journal of Adhesive Dentistry, 2001
• 新機能性Mg2+含有炭酸アパタイト・コラーゲン複合体の創製
  山崎 保彦; 吉田 靖弘; 岡崎 正之; 島津 篤; 濱田 吉之輔; 高橋 純造; 松浦 成昭
  日本バイオマテリアル学会大会予稿集, シンポジウム2000, 126, 126, 日本バイオマテリアル学会, Nov. 2000
  Japanese
• Assessment of decontamination methods as pretreatment of silanization of composite glass fillers
  K Shirai; Y Yoshida; Y Nakayama; M Fujitani; H Shintani; K Wakasa; M Okazaki; J Snauwaert; B Van Meerbeek
  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 53, 3, 204, 210, Jun. 2000
  English, Scientific journal
• Evidence of chemical bonding at biomaterial-hard tissue interfaces
  Y Yoshida; B Van Meerbeek; Y Nakayama; J Snauwaert; L Hellemans; P Lambrechts; G Vanherle; K Wakasa
  JOURNAL OF DENTAL RESEARCH, 79, 2, 709, 714, Feb. 2000
  English, Scientific journal
• 高分子バイオマテリアル 生体接着分子付与アパタイト・コラーゲン複合体の表面性状に関する研究
  山崎 保彦; 吉田 靖弘; 島津 篤; 岡崎 正之
  日本バイオマテリアル学会大会予稿集, 21回, 82, 82, 日本バイオマテリアル学会, Nov. 1999
  Japanese
• Silica investment prepared for dental purposes: effect of cristobalite content on mechanical properties
  K. Wakasa; A. Ikeda; Y. Yoshida; M. Yamaki
  Journal of Materials Science Letters, 12, 24, 1908, 1910, Kluwer Academic Publishers, Dec. 1993
  English, Scientific journal

• A Novel alveolar bone graft using polysaccharide derivative 'Phosphorylated Pullulan' for autologous bone augmentation material
  NAKANISHI K.; TAMADA I.; NAKAMURA M.; YOSHIDA Y.; SATO Y., 第84回日本矯正歯科学会学術大会 プログラム・抄録集, 147, 147, Sep. 2025
• 高齢者の口腔感染症の防止と介護者の負担軽減に資するCPC徐放・リチャージ義歯の開発
  中西康; 赤坂司; 吉田靖弘, 日本歯科医学会誌, 44, 2025
• Adhesion of composite resin cement to dentin
  吉原久美子; 丸尾幸憲; 長岡紀幸; 吉田靖弘, 接着歯学, 42, 1, 5, 9, Apr. 2024, [Peer-reviewed]
  Japan Society for Adhesive Dentistry, Japanese
• Bone Regeneration and Reconstruction Using Non-animal Derived Bioabsorbable Polymers
  吉田靖弘; 中西康; 赤坂司, 日本口腔インプラント学会誌, 37, 2, 103, 111, 2024
  Novel bioabsorbable materials have hardly progressed to clinical application ; collagen, hyaluronic acid, polyglycolic acid, and polylactic acid are still the mainstream. These bioabsorbable materials do not adhere to biological tissues. If bioabsorbable materials could be given the ability to adhere to biological tissues, the implantation of artificial bones and other mixtures into the affected area could be ensured. We have developed phosphorylated pullulan based on the tooth adhesive theory, which is the first non-animal-derived bioabsorbable material that adheres to bone and tooth. Phosphorylated pullulan is the only product selected for “Strategy of SAKIGAKE” by the Ministry of Health, Labour and Welfare in the dental field. We are planning to start an investigator-initiated clinical trial using phosphorylated pullulan as a class Ⅳ medical device in 2024. The mixture of phosphorylated pullulan and β-TCP has been reported to be useful as a putty-like bone replacement material for bone defects around dental implants. Practical applications are also being promoted outside the dental field. A submucosal injection material “enRise” used for endoscopic resection of gastric cancer, etc. has already received pharmaceutical approval. Phosphorylated pullulan has received various large amounts of support for research and development, probably because we have been focusing on introducing the new material from Japan to the world for the phosphorylated pullulan project. Other than the dental field, phosphorylated pullulan is expected to be used for scaffolds and carriers using drug delivery systems for various organs. Phosphorylated pullulan will be developed for a wide range of applications as new bioabsorbable materials instead of existing products., Japanese Society of Oral Implantology, Japanese
• 微細構造に応答した破骨細胞が作り出す多様なアクチン構造物
  赤坂 司; 中西 康; 吉田 靖弘, 日本歯科理工学会誌, 42, Special Issue WINTER, 5, 5, Dec. 2023
  (一社)日本歯科理工学会, Japanese
• 世界トップレベルの歯科材料・機器を臨床につなげる! 若手研究者の自由な発想と努力がもたらす大型医療研究事業
  吉田 靖弘, 日本補綴歯科学会誌, 15, 東北・北海道支部学術大会特別号, 15, 15, Oct. 2023
  (公社)日本補綴歯科学会, Japanese
• マイクロナノパターンが上皮細胞の接着に与える影響
  石井 咲苗; 吉田 靖弘; 横山 敦郎, 日本補綴歯科学会誌, 15, 東北・北海道支部学術大会特別号, 27, 27, Oct. 2023
  (公社)日本補綴歯科学会, Japanese
• Formation of Various Actin Structures by Osteoclast-like Cells in Response to Micro- and Nano-Patterns
  赤坂司; 宮治裕史; 横山敦郎; 吉田靖弘, 日本バイオマテリアル学会大会予稿集(Web), 45回, 422, 422, Oct. 2023
  日本バイオマテリアル学会, Japanese
• Bone regeneration and reconstruction using non-animal derived bioabsorbable polymers
  吉田靖弘, 日本口腔インプラント学会誌, 36, 特別号, 3, 3, Sep. 2023
  (公社)日本口腔インプラント学会, Japanese
• ものづくり研究の進め方 大型研究事業の企画立案と申請書作成のコツ
  吉田 靖弘, 北海道外科雑誌, 68, 1, 55, 56, Jun. 2023
  北海道外科学会, Japanese
• 臨床へ実装されるバイオロジー研究～研究室から診療室へ～ 未来歯科医療実現に向けた大型研究事業の活用
  吉田 靖弘, 日本補綴歯科学会誌, 15, 特別号, 84, 84, May 2023
  (公社)日本補綴歯科学会, Japanese
• マイクロナノパターンの表面性状が歯根膜線維芽細胞に与える影響
  工藤 円; 吉田 靖弘; 横山 敦郎, 日本補綴歯科学会誌, 15, 特別号, 262, 262, May 2023
  (公社)日本補綴歯科学会, Japanese
• 破骨細胞分化へのパターン材質と前駆細胞種の影響
  赤坂 司; 中西 康; 吉田 靖弘, 日本歯科理工学会誌, 42, Special Issue81, 78, 78, Mar. 2023
  (一社)日本歯科理工学会, Japanese
• Effect of surface structure with micro-nano pattern on fibroblast of periodontal ligament
  工藤円; 吉田靖弘; 横山敦郎, 日本補綴歯科学会誌(Web), 15, 2023
• Utilization of large-scale research projects for the realization of future dentistry
  吉田靖弘, 日本補綴歯科学会誌(Web), 15, 2023
• Nanoscale Analysis of the Tooth/Material Interface and Future Prospects for Adhesive Dentistry
  YOSHIHARA Kumiko; NAGAOKA Noriyuki; YOSHIDA Yasuhiro, Adhesive Dentistry, 40, 4, 141, 144, Dec. 2022
  Japan Society for Adhesive Dentistry, Japanese
• Efforts of large-scale research promotion committee―“Development of innovative dental research” learned from the large medical research promotion forum―
  Takano Yamamoto Teruko; Kusaka Teruo; Miyawaki Shouichi; Shimada Kouji; Nakanishi Ko; Yoshida Yasuhiro; Yamashiro Takashi, Journal of Japanese Dental Science Federation, 1, 1, 12, 16, Dec. 2022
  Japanese Dental Science Federation, Japanese
• 温度応答性歯科矯正用解体性接着材の開発
  春藤 彩花; 中西 康; 金子 知生; 黒川 考幸; 松本 愛子; 遠藤 諒俊; 赤坂 司; 吉田 靖弘; 佐藤 嘉晃, 日本矯正歯科学会大会プログラム・抄録集, 81回, 177, 177, Oct. 2022
  (公社)日本矯正歯科学会, Japanese
• 微細構造が破骨細胞への分化に与える影響
  赤坂 司; 中西 康; 吉田 靖弘, 北海道外科雑誌, 67, 1, 95, 95, Jun. 2022
  北海道外科学会, Japanese
• 抗菌性ポリウレタンゲル粘着シートの塩化セチルピリジニウム徐放特性と粘着性能
  中守 貴一; 阿部 泰彦; 吉原 久美子; 吉田 靖弘; 津賀 一弘, 日本歯科理工学会誌, 41, Special Issue79, 14, 14, May 2022
  (一社)日本歯科理工学会, Japanese
• パイロジェンフリー・非動物由来生体吸収性材料の開発
  中西 康; 赤坂 司; 吉原 久美子; 中村 真理子; 吉田 靖弘, 日本歯科理工学会誌, 41, Special Issue79, 13, 13, May 2022
  (一社)日本歯科理工学会, Japanese
• 破骨細胞様細胞への分化へ影響を与えるマイクロ・ナノパターンの探索
  赤坂 司; 横山 敦郎; 中西 康; 吉田 靖弘, 日本歯科理工学会誌, 41, Special Issue79, 31, 31, May 2022
  (一社)日本歯科理工学会, Japanese
• Dental innovation at Hokkaido University
  Yoshida Yasuhiro; Nakanishi Ko; Akasaka Tsukasa; Kusaka Teruo; Takano-Yamamoto Teruko, 日本歯科医学会連合雑誌, 1, 1, 17, 21, 2022
  Japanese Dental Science Federation, Japanese
• マイクロナノパターンの表面形状がヒト歯根膜線維芽細胞に与える影響について
  工藤 円; 吉田 靖弘; 横山 敦郎, 日本口腔インプラント学会誌, 34, 4, E215, E216, Dec. 2021
  (公社)日本口腔インプラント学会, Japanese
• マイクロナノパターンが骨形成に与える影響
  沼本 真一郎; 横山 敦郎; 吉田 靖弘, 日本口腔インプラント学会誌, 34, 特別号, ポスター, 30, Dec. 2021
  (公社)日本口腔インプラント学会, Japanese
• 骨再生におけるバイオマテリアル開発と骨質評価アップデート 新規リン酸化多糖体"リン酸化プルラン"を用いた骨再生(Bone regeneration by using the novel phosphorylated polysaccharide "Phosphorylated pullulan")
  長谷川 智香; 森本 康仁; 久保田 恵亮; 本郷 裕美; 吉田 靖弘; 網塚 憲生, Journal of Oral Biosciences Supplement, 2021, 90, 90, Oct. 2021
  (一社)歯科基礎医学会, Japanese
• 骨補填材としてのリン酸化プルラン/β-TCPコンビネーションマテリアルの応用
  森本 康仁; 久保田 恵亮; 阿部 未来; 丸岡 春日; 長谷川 智香; 本郷 裕美; 吉田 靖弘; 網塚 憲生; 菅谷 勉, 日本歯周病学会会誌, 63, 秋季特別, 120, 120, Oct. 2021
  (NPO)日本歯周病学会, Japanese
• 【歯科生体材料の進歩が歯科臨床の未来を拓く!】大学発の新技術が歯科医療のプレゼンスを高める
  吉田 靖弘; 横山 敦郎; 阿部 泰彦, 歯科医療, 35, 4, 28, 38, Oct. 2021
  (株)第一歯科出版, Japanese
• 歯科理工学 東西対抗戦 東軍 北海道大学におけるデンタルイノベーション戦略
  吉田 靖弘, 日本歯科理工学会誌, 40, Special Issue78, 5, 5, Sep. 2021
  (一社)日本歯科理工学会, Japanese
• 破骨細胞の吸収窩形成へのマイクロ・ナノパターンの影響
  赤坂 司; 中西 康; 吉田 靖弘, 日本歯科理工学会誌, 40, Special Issue77, 31, 31, Mar. 2021
  (一社)日本歯科理工学会, Japanese
• 唇顎口蓋裂治療における外科的侵襲の低減と医療費適正化
  吉田 靖弘; 岡部 一登, 医科学応用研究財団研究報告, 38, 163, 166, Feb. 2021
  (公財)鈴木謙三記念医科学応用研究財団, Japanese
• 骨補填材としてのリン酸化プルラン/β-TCPコンビネーションマテリアルの応用
  森本康仁; 久保田恵亮; 阿部未来; 丸岡春日; 長谷川智香; 本郷裕美; 吉田靖弘; 網塚憲生; 菅谷勉, 日本歯周病学会会誌(Web), 63, 2021
• Bone regeneration by using the novel phosphorylated polysaccharide “Phosphorylated pullulan”
  長谷川智香; 森本康仁; 久保田恵亮; 本郷裕美; 吉田靖弘; 網塚憲生, Journal of Oral Biosciences Supplement (Web), 2021, 2021
• Examination of bond strength of resin cement to additive manufacturing zirconia
  吉原久美子; 清水那弥; 長岡紀幸; 吉田靖弘, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 154th, 84, 84, 2021
  (NPO)日本歯科保存学会, Japanese
• リン酸三カルシウム(TCP)およびリン酸化プルラン(PPL)含有新規骨補填材を用いた骨再生
  森本康仁; 長谷川智香; 本郷裕美; 久保田恵亮; 阿部未来; 丸岡春日; 吉田靖弘; 菅谷勉; 網塚憲生, 日本再生医療学会総会(Web), 20th, 2021
• リン酸化多糖体とTCP混合補填材を用いたインプラント周囲骨再生における組織学的検索
  久保田恵亮; 長谷川智香; 本郷裕美; 森本康仁; 阿部未来; 丸岡春日; 吉田靖弘; 横山敦郎; 網塚憲生, 日本再生医療学会総会(Web), 20th, 2021
• リン酸化プルラン/β-TCPコンビネーションマテリアルにおける新規骨再生誘導
  森本康仁; 久保田恵亮; 阿部未来; 丸岡春日; 本郷裕美; 吉田靖弘; 菅谷勉; 網塚憲生; 長谷川智香, 日本骨形態計測学会雑誌, 31, 2, S135, S135, 2021
  日本骨形態計測学会, Japanese
• マイクロ・ナノパターンが骨系細胞に与える影響について
  沼本真一郎; 横山敦郎; 赤坂司; 吉田靖弘, 北海道外科雑誌, 66, 1, 57, 57, 2021
  北海道外科学会, Japanese
• リン酸化プルラン含有根管充填シーラーの封鎖性と根尖病巣の治療効果
  大嶌 理紗; 河野 通俊; 下地 伸司; 菅谷 勉; 吉田 靖弘, 北海道外科雑誌, 65, 1, 91, 92, Jun. 2020
  北海道外科学会, Japanese
• 化学重合ユニバーサルボンドのデュアルキュア化の検討
  吉原 久美子; 長岡 紀幸; 入江 正郎; 丸尾 幸憲; 西川 悟郎; 吉田 靖弘, 日本補綴歯科学会誌, 12, 特別号, 92, 92, Jun. 2020
  (公社)日本補綴歯科学会, Japanese
• リン酸化プルラン含有根管充填シーラーの封鎖性と根尖病巣の治療効果
  大嶌 理紗; 河野 通俊; 下地 伸司; 菅谷 勉; 吉田 靖弘, 北海道外科雑誌, 65, 1, 91, 92, Jun. 2020
  北海道外科学会, Japanese
• 破骨細胞分化誘導へのマイクロ・ナノパターンの影響
  赤坂 司; 玉井 美保; 吉田 靖弘, 日本歯科理工学会誌, 39, Special Issue75, 8, 8, Apr. 2020
  (一社)日本歯科理工学会, Japanese
• Development of self-adhesive pulp-capping agents containing functional monomer
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 152nd, 107, 107, 2020
  (NPO)日本歯科保存学会, Japanese
• Examination of dual cure system of chemical cure universal adhesive
  吉原久美子; 長岡紀幸; 入江正郎; 丸尾幸憲; 西川悟郎; 吉田靖弘, 日本補綴歯科学会誌(Web), 12, 2020
• リン酸三カルシウム(TCP)/リン酸化プルラン(PPL)含有新規骨補填材による新生骨形成誘導について
  本郷裕美; 森本康仁; 長谷川智香; 菅谷勉; 吉田靖弘; 網塚憲生, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 125th, 2020
• リン酸三カルシウム(TCP)/リン酸化プルラン(PPL)含有新規骨補填材によって形成された新生骨の組織学的検索
  本郷裕美; 長谷川智香; 森本康仁; 菅谷勉; 吉田靖弘; 網塚憲生, 日本再生医療学会総会(Web), 19th, 2020
• ナノ多孔質シリカの孔サイズがもたらすイオン放出能への影響
  遠藤 諒俊; 中西 康; 坂東 洋祐; 松本 愛子; 阿部 薫明; 山方 秀一; 吉田 靖弘; 佐藤 嘉晃, 日本矯正歯科学会大会プログラム・抄録集, 78回, 192, 192, Nov. 2019
  (公社)日本矯正歯科学会, Japanese
• リン酸三カルシウム(TCP)およびリン酸化プルラン(PPL)含有新規骨補填材の開発
  森本 康仁; 長谷川 智香; 本郷 裕美; 吉田 靖弘; 網塚 憲生; 菅谷 勉, 日本歯周病学会会誌, 61, 秋季特別, 142, 142, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• 液体アクリルアミド系モノマー含有接着材の検討
  吉原 久美子; 長岡 紀幸; 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue74, 70, 70, Sep. 2019
  (一社)日本歯科理工学会, Japanese
• マイクロ・ナノ構造への血液関連細胞の付着性
  赤坂 司; 玉井 美保; 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue74, 34, 34, Sep. 2019
  (一社)日本歯科理工学会, Japanese
• ナノポーラス物質含有による歯科用シーラント材料から薬剤徐放特性の検討
  江良 裕子; 中西 康; 阿部 薫明; 玉井 美保; 赤坂 司; 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue74, 47, 47, Sep. 2019
  (一社)日本歯科理工学会, Japanese
• ナノポーラスシリカを用いた新規な薬剤徐放能性歯科材料の開発
  江良 裕子; 成徳 英理; 中西 康; 坂東 洋祐; 阿部 薫明; 吉田 靖弘, 北海道外科雑誌, 64, 1, 101, 102, Jun. 2019
  北海道外科学会, Japanese
• リン酸化プルラン含有MTAシーラーが実験的根尖性歯周炎の治癒に及ぼす効果
  河野通俊; 大嶌理紗; 中塚 愛; 中谷充宣; 前田良子; 下地伸司; 菅谷 勉; 吉田靖弘, 第150回春季日本歯科保存学会学術大会．プログラムおよび講演抄録集, 150回, 39, 39, Jun. 2019
  (NPO)日本歯科保存学会, Japanese
• CPC担持モンモリロナイト含有抗菌性ティッシュコンディショナーの性能と安全性
  朝原 恵里加; 直江 翼; 槇田 洋二; 岡崎 洋平; 保田 啓介; 中守 貴一; 岡田 信輔; 堀内 留美; 吉田 靖弘; 横山 敦郎; 津賀 一弘; 阿部 泰彦, 日本補綴歯科学会誌, 11, 特別号, 169, 169, May 2019
  (公社)日本補綴歯科学会, Japanese
• 新規シランカップリング剤添加型レジンセメントのシランカップリング効果
  吉原 久美子; 長岡 紀幸; 丸尾 幸憲; 西川 悟郎; 吉田 靖弘, 日本補綴歯科学会誌, 11, 特別号, 141, 141, May 2019
  (公社)日本補綴歯科学会, Japanese
• 液体アクリルアミド系モノマー含有レジン添加型覆髄材の検討
  吉原 久美子; 長岡 紀幸; 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue73, 14, 14, Apr. 2019
  (一社)日本歯科理工学会, Japanese
• カゴ状ナノシリカ粒子を用いた薬剤徐放性歯科材料の開発
  江良 裕子; 阿部 薫明; 赤坂 司; 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue73, 43, 43, Apr. 2019
  (一社)日本歯科理工学会, Japanese
• Groove/Pillarパターン化チタン表面が細胞接着及び増殖に与える影響
  加我 公行; 赤坂 司; 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue73, 37, 37, Apr. 2019
  (一社)日本歯科理工学会, Japanese
• 核反応を利用したフッ化物含有接着材からの歯質内フッ素分布の測定
  奥山 克史; 松田 康裕; 山本 洋子; 新谷 耕平; 堀口 敬司; 斉藤 隆史; 林 美加子; 吉田 靖弘; 玉置 幸道, 日本歯科理工学会誌, 38, Special Issue73, 65, 65, Apr. 2019, [Peer-reviewed]
  (一社)日本歯科理工学会, Japanese
• マイクロ・ナノ構造が歯周組織関連細胞の挙動にもたらす影響
  高田 亮; 赤坂 司; 山方 秀一; 佐藤 嘉晃; 吉田 靖弘, 北海道歯学雑誌, 39, 2, 167, 167, Mar. 2019
  北海道歯学会, Japanese
• マイクロ・サブミクロパターン構造を付与したCOP film上における細胞挙動
  本間 淳; 赤坂 司; 山方 秀一; 佐藤 嘉晃; 吉田 靖弘, 北海道歯学雑誌, 39, 2, 167, 167, Mar. 2019
  北海道歯学会, Japanese
• CPC担持モンモリロナイト含有抗菌性ティッシュコンディショナーの性能と安全性
  朝原恵里加; 直江翼; 槇田洋二; 岡崎洋平; 保田啓介; 中守貴一; 岡田信輔; 堀内留美; 吉田靖弘; 横山敦郎; 津賀一弘; 阿部泰彦, 日本補綴歯科学会誌(Web), 11, 2019
• 新規シランカップリング剤添加型レジンセメントのシランカップリング効果
  吉原久美子; 長岡紀幸; 丸尾幸憲; 西川悟郎; 吉田靖弘, 日本補綴歯科学会誌(Web), 11, 2019
• 新規親水性アミドモノマーの接着材への応用の検討
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本接着歯学会学術大会講演集(Web), 38th, 3, 126, 126, 2019
  (一社)日本接着歯学会, Japanese
• リン酸化ポリビニルアルコールのグラスアイオノマーセメントへの応用
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 151st, 91, 91, 2019
  (NPO)日本歯科保存学会, Japanese
• ユニバーサルボンディング材のI級窩洞における象牙質接着強さ
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 150th, 115, 115, 2019
  (NPO)日本歯科保存学会, Japanese
• マイクロ・ナノパターン表面構造における細胞応答
  加我公行; 赤坂司; 吉田靖弘; 横山敦郎, 北海道外科雑誌, 64, 1, 101, 101, 2019
  北海道外科学会, Japanese
• リン酸三カルシウム(TCP)およびリン酸化プルラン(PPL)含有新規骨補填剤による新生骨誘導における組織学的解析
  森本康仁; 長谷川智香; 邱紫せん; 松本愛子; 桐越晶子; 森谷康人; 橋本圭司; 趙申; 前壮功仁; 本郷裕美; 菅谷勉; 吉田靖弘; 網塚憲生, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 124th, 162, 2019
  Japanese
• 矯正歯科用ステンレススチールワイヤーへのイオン溶出抑制を目的とした表面化学修飾
  玉木 宏典; 阿部 薫明; 山方 秀一; 佐藤 嘉晃; 飯田 順一郎; 吉田 靖弘, 日本矯正歯科学会大会プログラム・抄録集, 77回, 253, 253, Oct. 2018
  (公社)日本矯正歯科学会, Japanese
• マイクロ・ナノ構造が細胞接着にもたらす影響
  高田 亮; 赤坂 司; 山方 秀一; 佐藤 嘉晃; 飯田 順一郎; 吉田 靖弘, 日本矯正歯科学会大会プログラム・抄録集, 77回, 256, 256, Oct. 2018
  (公社)日本矯正歯科学会, Japanese
• ワンステップ接着システムにおける歯質界面の生成物と物性の関係
  鈴木 一臣; 長岡 紀幸; 吉原 久美子; 松本 卓也; 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue72, 29, 29, Sep. 2018
  (一社)日本歯科理工学会, Japanese
• 化学重合型マルチプライマーの歯質接着界面の解析
  吉原 久美子; 長岡 紀幸; 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue72, 30, 30, Sep. 2018
  (一社)日本歯科理工学会, Japanese
• 多孔質シリカを用いた選択的薬剤徐放を実現する歯科用材料の開発
  江良 裕子; 中西 康; 阿部 薫明; 玉井 美保; 赤坂 司; 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue72, 56, 56, Sep. 2018
  (一社)日本歯科理工学会, Japanese
• ユウロピウム錯体を用いた矯正歯科用蛍光ボンディング材の試作
  松本 愛子; 山方 秀一; 佐藤 嘉晃; 阿部 薫明; 赤坂 司; 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue72, 107, 107, Sep. 2018
  (一社)日本歯科理工学会, Japanese
• ワンステップ接着システムにおける歯質界面の生成物と物性の関係
  鈴木 一臣; 長岡 紀幸; 吉原 久美子; 松本 卓也; 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue72, 29, 29, Sep. 2018
  (一社)日本歯科理工学会, Japanese
• 化学重合型マルチプライマーの歯質接着界面の解析
  吉原 久美子; 長岡 紀幸; 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue72, 30, 30, Sep. 2018
  (一社)日本歯科理工学会, Japanese
• ナノ多孔質シリカを用いた新規多機能性グラスアイオノマーセメントの開発
  坂東 洋祐; 中西 康; 山方 秀一; 飯田 順一郎; 阿部 薫明; 吉田 靖弘, 北海道外科雑誌, 63, 1, 63, 63, Jun. 2018
  北海道外科学会, Japanese
• 北大歯におけるイノベーション・ベンチャー戦略
  吉田 靖弘, 北海道外科雑誌, 63, 1, 67, 67, Jun. 2018
  北海道外科学会, Japanese
• 歯科材料に混合したナノポーラス物質からの薬剤徐放特性の追跡
  江良 裕子; 中西 康; 阿部 薫明; 赤坂 司; 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue71, 53, 53, Mar. 2018
  (一社)日本歯科理工学会, Japanese
• プライマー接触硬化型レジンセメント重合のリアルタイム測定
  吉原 久美子; 長岡 紀幸; 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue71, 77, 77, Mar. 2018
  (一社)日本歯科理工学会, Japanese
• 薬剤徐放能を持つ多孔質シリカを用いた歯科用材料の開発
  江良裕子; 坂東洋祐; 中西康; 阿部薫明; 玉井美保; 赤坂司; 飯田順一郎; 吉田靖弘, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018
• モノマーカルシウム塩の層状構造の三次元観察と接着界面の弾性率評価
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本接着歯学会学術大会講演集(Web), 37th, 3, 111, 111, 2018
  (一社)日本接着歯学会, Japanese
• 新規抗菌材添加セメントのバイオフィルム形成抑制特性
  吉原久美子; 長岡紀幸; 槇田洋二; 山本裕也; 吉田靖弘, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 149th, 72, 72, 2018
  (NPO)日本歯科保存学会, Japanese
• 新規多糖誘導体リン酸化プルランフィルムとBMP-2の併用はラット脊椎固定モデルにおいて骨癒合を促進する
  宇川諒; 塩崎泰之; 村岡聡介; 辻寛謙; 吉田晶; 瀧川朋亨; 三澤治夫; 吉原久美子; 吉田靖弘; 尾崎敏文; 松川昭博, 整形外科バイオマテリアル研究会プログラム・抄録集, 38th, 2018
• 歯質に対する被着面処理 2 象牙質に対する被着面処理 2)プライマー処理 接着性モノマーによる象牙質接着メカニズムの解明
  吉田靖弘, 日本接着歯学会設立35周年記念誌 平成30年, 47‐48, 2018
  Japanese
• ナノ構造体シリカからの薬剤徐放挙動と生体適合性の評価
  阿部 薫明; 中西 康; 坂東 洋祐; 成徳 英理; 江良 裕子; 飯田 順一郎; 佐野 英彦; 吉田 靖弘, 日本バイオマテリアル学会大会予稿集, 39回, 243, 243, Nov. 2017
  日本バイオマテリアル学会, Japanese
• 歯科矯正用ステンレススチールワイヤーの化学修飾による金属イオン溶出抑制
  土屋 聡; 阿部 薫明; 山方 秀一; 吉田 靖弘; 飯田 順一郎, 日本矯正歯科学会大会プログラム・抄録集, 76回, 205, 205, Oct. 2017
  (公社)日本矯正歯科学会, Japanese
• レジンセメントの曲げ強さに対する長期水中保管の影響
  吉原 久美子; 長岡 紀幸; 入江 正郎; 吉田 靖弘, 接着歯学, 35, 3, 68, 68, Oct. 2017
  (一社)日本接着歯学会, Japanese
• アルカリ環境が修復材料の耐久性に与える影響
  吉原 久美子; 長岡 紀幸; 小河 達之; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 147回, 156, 156, Oct. 2017
  (NPO)日本歯科保存学会, Japanese
• 学識経験者の寄稿 歯科発のシーズを活かした医療イノベーション
  吉田 靖弘, ちゅうごく産業創造センター会報, 114, 4, 8, 26 Sep. 2017
  広島 : ちゅうごく産業創造センター, Japanese
• ナノポーラス物質を用いたグラスアイオノマーセメントマトリックスからの薬剤モデル物質の徐放挙動
  江良 裕子; 中西 康; 阿部 薫明; 玉井 美保; 赤坂 司; 吉田 靖弘, 日本歯科理工学会誌, 36, 5, 356, 356, Sep. 2017
  (一社)日本歯科理工学会, Japanese
• う蝕治療に使用される歯質接着材の電子顕微鏡観察
  長岡紀幸; 吉原久美子; 吉田靖弘, 顕微鏡, 52, 2, 104‐107, 107, 30 Aug. 2017
  The general treatment for a dental caries is to remove the decay and put in a filling artificial materials. Recently, tooth color composite resin is often used for filling material due to meet patients’ esthetic demand. Composite resin doesn’t adhere tooth itself, so dental adhesive is needed. It is known that the components of dental adhesive affect the longevity and clinical performance. We focused the interface between tooth and adhesive using transmission electron microscopy. Furthermore, we investigated the interaction functional monomer in dental adhesive and hydroxyapatite using X-ray diffraction (XRD), solid state nuclear magnetic resonance (NMR)., The Japanese Society of Microscopy, Japanese
• 10-MDPプライマー処理前の洗浄がジルコニアの接着に及ぼす影響
  吉原 久美子; 長岡 紀幸; 丸尾 幸憲; 吉田 靖弘, 日本補綴歯科学会誌, 9, 特別号, 248, 248, Jun. 2017
  (公社)日本補綴歯科学会, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーの根尖封鎖性
  大嶌理紗; 下地伸司; 菅谷 勉; 吉田靖弘, 第146回春季日本歯科保存学会学術大会．プログラムおよび講演抄録集, 146回, 40, 40, Jun. 2017
  (NPO)日本歯科保存学会, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーによる根尖孔への硬組織形成
  河野通俊; 大嶌理紗; 熊谷広道; 工藤 愛; 佐藤賢人; 中谷充宣; 前田良子; 大歳祐生; 下地伸司; 菅谷 勉; 吉田靖弘, 第146回春季日本歯科保存学会学術大会．プログラムおよび講演抄録集, 146回, 139, 139, Jun. 2017
  (NPO)日本歯科保存学会, Japanese
• 異なる種類のフィラーが1液型ボンディング材の粘性、耐久性に与える影響
  吉原 久美子; 小河 達之; 長岡 紀幸; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 146回, 90, 90, May 2017
  (NPO)日本歯科保存学会, Japanese
• 硬化可能な歯科材料によるマイクロ・ナノパターニング
  赤坂 司; 加我 公行; 横山 敦郎; 阿部 薫明; 吉田 靖弘, 日本歯科理工学会誌, 36, 2, 82, 82, Mar. 2017
  (一社)日本歯科理工学会, Japanese
• The development of acrylamide monomer containing pulp capping materials
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科理工学会誌, 36, 2, 141, 141, Mar. 2017
  (一社)日本歯科理工学会, Japanese
• 臨床医がもとめるコンビネーションプロダクト~歯科領域を中心に~
  吉田靖弘, 月刊PHARM STAGE, 16, 11, 58‐62, 62, 15 Feb. 2017
  技術情報協会, Japanese
• Cell population of mesenchymal stem cells on micro-patterned titanium
  Mariko Kawai; Noriyuki Nagaoka; Yasuhiro Yoshida; Kiyoshi Ohura, World Congress on Recent Advances in Nanotechnology, 2017
  Avestia Publishing, English
• 10-MDPプライマー処理前の洗浄がジルコニアの接着に及ぼす影響
  吉原久美子; 長岡紀幸; 丸尾幸憲; 吉田靖弘, 日本補綴歯科学会誌(Web), 9, 2017
• Micro/nano-patterning of gelatin by crosslinking with genipin
  赤坂司; 阿部薫明; 吉田靖弘, 日本歯科理工学会誌, 36, 5, 351, 351, 2017
  (一社)日本歯科理工学会, Japanese
• Density functional theory study on oligosilane-functionalized C
  Yoshizawa Saori; Abe Shigeaki; Mutoh Mami; Kusaka Teruo; Nakamura Mariko; Yoshida Yasuhiro; Iida Junichiro; Kawabata Hiroshi; Tachikawa Hiroto, Jpn. J. Appl. Phys., 56, 1, 01AE03, 08 Nov. 2016
  Oligosilane-functionalized C<inf>60</inf>fullerenes [namely, C<inf>60</inf>–(SiH<inf>2</inf>)<inf>n</inf>–H, n = 1–4] have been investigated by the density functional theory (DFT) method to elucidate the structures and electronic states of oligosilane-radical added fullerene. The DFT calculation showed that oligosilane radicals bind to the carbon atom of C<inf>60</inf>in the on-top site, and a strong Si–C heterojunction is formed. The binding energies of oligosilane radicals to C<inf>60</inf>were calculated to be 24.6–28.2 kcal/mol at the CAM-B3LYP/6-311G(d,p) level. The electronic states of oligosilane-functionalized fullerenes C<inf>60</inf>–(SiH<inf>2</inf>)<inf>n</inf>–H are discussed on the basis of theoretical results., Institute of Physics, English
• 蛍光性セラミックスナノ粒子の細胞への適合性の評価
  木山 望; 阿部 薫明; 山方 秀一; 吉田 靖弘; 飯田 順一郎, 日本矯正歯科学会大会プログラム・抄録集, 75回, 211, 211, Nov. 2016
  (公社)日本矯正歯科学会, Japanese
• 新しい接着領域への探求 接着歯学の基礎研究が生んだセラピューティック・イノベーション
  吉田 靖弘, 接着歯学, 34, 3, 94, 94, Nov. 2016
  (一社)日本接着歯学会, Japanese
• ヒト由来細胞に対するセラミックナノ粒子の生体適合性の検討
  阿部 薫明; 成徳 英理; 江良 裕子; 吉田 靖弘; 佐野 英彦, 日本バイオマテリアル学会大会予稿集, シンポジウム2016, 138, 138, Nov. 2016
  日本バイオマテリアル学会, Japanese
• ジルコニアに対するカップリングモノマーの吸着特性
  長岡 紀幸; 吉原 久美子; 吉田 靖弘, 接着歯学, 34, 3, 99, 99, Nov. 2016
  (一社)日本接着歯学会, Japanese
• 2ステップセルフエッチングボンディング材の長期臨床症例の接着界面観察
  吉原 久美子; 長岡 紀幸; 吉田 靖弘, 接着歯学, 34, 3, 107, 107, Nov. 2016
  (一社)日本接着歯学会, Japanese
• バイオアクティブガラス含有歯質接着材の電子顕微鏡観察
  長岡 紀幸; 吉原 久美子; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 145回, 36, 36, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• リン酸エッチングの有無がユニバーサルアドヒーシブの象牙質接着耐久性に与える影響
  吉原 久美子; 長岡 紀幸; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 145回, 74, 74, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• 接着性を有するMTA含有直接覆髄材の開発
  戸井田 侑; 川野 晋平; 丁 世俊; 長谷川 智香; チョウドリ・アルマス; サイケオ・ピポップ; アハメッド・ズバエル; 成徳 英理; 勝俣 愛一郎; 福澤 尚幸; 松本 真理子; 角田 晋一; 星加 修平; 池田 考績; 田中 享; 島田 康史; 田上 順次; 網塚 憲生; 吉田 靖弘; 佐野 英彦, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 145回, 24, 24, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• 多糖複合体を基材とした感染制御能と生体吸収性を有する抗菌薬含有骨セメントの開発
  渡邉典行; 香川洋平; 張偉; 吉村将秀; 吉田晶; 田中雅人; 吉田靖弘; 松川昭博; 尾崎敏文, 日本整形外科学会雑誌, 90, 8, S1819, S1819, 31 Aug. 2016
  (公社)日本整形外科学会, Japanese
• 新規生体材料リン酸化プルランによるインプラントコーティング技術の開発
  香川洋平; 渡邉典行; 張偉; 吉村将秀; 吉田晶; 吉田靖弘; 尾崎敏文; 松川昭博, 日本整形外科学会雑誌, 90, 8, S1813, S1813, 31 Aug. 2016
  (公社)日本整形外科学会, Japanese
• マイクロ・ナノパターン化ゼラチンシートへの細胞接着に対する影響
  加我 公行; 横山 敦郎; 吉田 靖弘, 日本補綴歯科学会誌, 8, 特別号, 299, 299, Jul. 2016
  (公社)日本補綴歯科学会, Japanese
• リチウムシリケートセラミックの構造観察と分析
  吉原 久美子; 長岡 紀幸; 丸尾 幸憲; 西川 悟郎; 吉田 靖弘, 日本補綴歯科学会誌, 8, 特別号, 234, 234, Jul. 2016
  (公社)日本補綴歯科学会, Japanese
• マイクロ・ナノパターン化ゼラチンシートへの細胞接着に対する影響
  加我公行; 横山敦郎; 吉田靖弘, 日本補綴歯科学会誌(Web), 8, 299 (WEB ONLY), Jul. 2016
  Japanese
• Bioactive glassフィラー含有コンポジットレジンの表面性状の検討
  吉原 久美子; 長岡 紀幸; 佐野 英彦; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 144回, 83, 83, Jun. 2016
  (NPO)日本歯科保存学会, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーの封鎖性に及ぼす根管洗浄剤の影響
  大嶌理紗; 菅谷 勉; 下地伸司; 竹生寛恵; 川浪雅光; 吉田靖弘, 第144回春季日本歯科保存学会学術大会．プログラムおよび講演抄録集, 144回, 110, 110, Jun. 2016
  (NPO)日本歯科保存学会, Japanese
• 歯表面での3D微細構造構築によるバイオミメティック次世代歯科治療へ向けて
  赤坂司; 加我公行; 宮治裕史; 横山敦郎; 吉田靖弘, 日本歯科医学会誌, 35, 36, 36, 31 Mar. 2016
  日本歯科医学会, Japanese
• Electroconductive treatment for SEM observation of wet-biological samples using room temperature ionic liquids-
  江良裕子; 阿部薫明; 川上隼人; 河合功治; 赤坂司; 吉田靖弘, 日本歯科理工学会誌, 35, 2, 135, 135, 25 Mar. 2016
  (一社)日本歯科理工学会, Japanese
• Cell aggregation of periodontal ligament fibroblasts controlled by micro-patterns
  赤坂司; 加我公行; 横山敦郎; 阿部薫明; 吉田靖弘, 日本歯科理工学会誌, 35, 2, 134, 134, 25 Mar. 2016
  (一社)日本歯科理工学会, Japanese
• A novel drug controlled release system using phoshorylated polysaccharides
  阿部薫明; 沖原巧; 赤坂司; 吉田靖弘, 日本歯科理工学会誌, 35, 2, 128, 128, 25 Mar. 2016
  (一社)日本歯科理工学会, Japanese
• The development of acrylamide monomer containing self-adhesive resin sealer
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科理工学会誌, 35, 2, 82, 82, 25 Mar. 2016
  (一社)日本歯科理工学会, Japanese
• Evaluation of cytocompatibility of montmotillonite for novel dental materials
  中西康; 阿部薫明; 坂東洋祐; 山方秀一; 吉田靖弘; 飯田順一郎, 日本歯科理工学会誌, 35, 2, 139, 139, 25 Mar. 2016
  (一社)日本歯科理工学会, Japanese
• Evaluation of Staining Inhibition and Adsorption of Sodium Metaphosphate to Bleached Enamel
  牧野紗織; 川本千春; 池田考績; 吉原久美子; 吉田靖弘; 佐野英彦, 日本歯科保存学雑誌, 59, 1, 22‐31, 31, 29 Feb. 2016
  (NPO)日本歯科保存学会, Japanese
• Electronic states of alkyl-radical-functionalized C
  Abe Shigeaki; Kawano Shimpei; Toida Yu; Nakamura Mariko; Inoue Satoshi; Sano Hidehiko; Yoshida Yasuhiro; Kawabata Hiroshi; Tachikawa Hiroto, Jpn. J. Appl. Phys., 55, 3, 03DD03, 03DD03, 19 Jan. 2016
  The structures and electronic states of alkyl-radical-functionalized C<inf>20</inf>fullerenes (denoted by C<inf>20</inf>–R) have been investigated using density functional theory (DFT). The different alkyl radicals investigated were methyl, ethyl, propyl, and butyl radicals. The DFT calculation indicated that the alkyl radical binds to the carbon atom of C<inf>20</inf>in the on-top site, thus forming a strong C–C single bond. The binding energies of the alkyl radicals to C<inf>20</inf>were calculated to be 83.9–86.6 kcal/mol at the CAM-B3LYP/6-311G(d,p) level. The electronic states of the C<inf>20</inf>–R complex are discussed on the basis of the theoretical results., Institute of Physics
• 従来型グラスアイオノマーセメントの電子顕微鏡観察
  長岡紀幸; 吉原久美子; 吉田靖弘, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 144th, 89, 89, 2016
  (NPO)日本歯科保存学会, Japanese
• 酵素モデル化合物を用いた石灰化反応制御
  阿部薫明; 吉沢早織; 吉田靖弘; 飯田順一郎, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, ROMBUNNO.P42, 2016
  Japanese
• 多孔性シリカ添加グラスアイオノマーセメントのイオン放出能の検討
  中西康; 坂東洋祐; 阿部薫明; 山方秀一; 吉田靖弘; 飯田順一郎, 日本矯正歯科学会大会プログラム・抄録集, 75th, 213, 213, 2016
  (公社)日本矯正歯科学会, Japanese
• リン酸系モノマーのアパタイト・象牙質への化学的相互作用
  吉原久美子; 長岡紀幸; 井上哲; 吉田靖弘, 接着歯学, 33, 4, 196‐203, 203, 15 Dec. 2015
  歯科治療における象牙質の接着には、樹脂含浸層の形成による機械的嵌合に加え、化学的な結合が関与する。特に化学的な結合に関しては、機能性モノマーの構造や純度が影響する。また、HEMAなど他のモノマーが共存する場合では、歯質との反応性が異なることも報告されている。異なるスペーサーの長さを持つ機能性モノマーの化学的反応性、機能性モノマーの純度が接着性・接着耐久性に与える影響、フッ素を含有した機能性モノマーについて述べた。, Japan Society for Adhesive Dentistry, Japanese
• 新規リン酸モノマー含有ボンディング材の歯質接着性の評価
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本接着歯学会学術大会講演集(Web), 34th, 3, P16 (WEB ONLY), 141, 15 Nov. 2015
  (一社)日本接着歯学会, Japanese
• ハイブリッドレジンの構造とサンドブラストの影響
  長岡紀幸; 吉原久美子; 吉田靖弘, 日本接着歯学会学術大会講演集(Web), 34th, 3, P32 (WEB ONLY), 149, 15 Nov. 2015
  (一社)日本接着歯学会, Japanese
• 10-MDP含有レジンセメントの象牙質接着界面の解析
  吉原 久美子; 長岡 紀幸; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 143回, 101, 101, Nov. 2015
  (NPO)日本歯科保存学会, Japanese
• 新規生体吸収性骨セメントの細胞動態の検討
  ZHANG W; 吉田晶; 山根健太郎; 香川洋平; 篠原健介; 吉田靖弘; 松川昭博; 尾崎敏文, 日本整形外科学会雑誌, 89, 8, S1575, S1575, 10 Sep. 2015
  (公社)日本整形外科学会, Japanese
• 新規生体材料によるインプラントコーティング
  香川 洋平; 山根 健太郎; 篠原 健介; 渡邉 典行; 張 偉; 吉田 晶; 吉田 靖弘; 尾崎 敏文; 松川 昭博, 日本整形外科学会雑誌, 89, 8, S1576, S1576, Sep. 2015
  (公社)日本整形外科学会, Japanese
• 多糖複合体リン酸化プルランを用いた抗菌薬含有骨セメントの開発
  渡邉 典行; 山根 健太郎; 香川 洋平; 篠原 健介; 張 偉; 吉田 晶; 松川 昭博; 吉田 靖弘; 田中 雅人; 尾崎 敏文, 日本整形外科学会雑誌, 89, 8, S1577, S1577, Sep. 2015
  (公社)日本整形外科学会, Japanese
• P-53 Cell alignment and migration of periodontal ligament fibroblasts on micro-patterns
  Akasaka T; Kaga N; Yokoyama A; Abe S; Yoshida Y, The journal of the Japanese Society for Dental Materials and Devices, 34, 5, 397, 397, Sep. 2015
  We investigated preparation of micro/nano-pattered composite on the curved surface of artificial tooth. And then the behaviors of cell alignment and movement on micro-pattern were estimated using human periodontal ligament fibroblasts (HPDLF). As a result, patterning to the curved surface was able to be obtained by using flexible mold. The alignment and movement of cells was influenced by type of patterns consisting of groove. These data supported that the micro/nano-pattern can control the alignment and assembly of cells., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• The development of long-term effectiveness silver ion-loaded antimicrobial agent
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科理工学会誌, 34, 5, 351, 351, Sep. 2015
  It has been known that Silver has antibacterial effect on gram-positive and gram-negative bacteria. Several dental materials contain silver, however the antibacterial effect of silver is not so high. So we synthesized novel silver-amino acid complexes and analyzed their antibacterial efficacy. These materials were characterized by SEM and XRD. Then the antibacterial efficacy on S. mutans were investigated. The stability of these materials in salts water were investigated by measuring silver ion. The stability test revealed especially His-Ag-montmorillonite showed strong suppressing production of silver chloride. The structure of silver-complex affected the antibacterial efficacy. His-Ag-montmorillonite can be useful for dental materials., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• CAD/CAMレジンブロックに対するサンドブラスト処理の影響
  吉原久美子; 長岡紀幸; 入江正郎; 松本卓也; 吉田靖弘, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 142nd, P21 (WEB ONLY), 96, 01 Jun. 2015
  (NPO)日本歯科保存学会, Japanese
• 生体触媒安定化剤への利用に向けた高溶解性イオン液体中での酵素の長期安定性の検討
  金子恒太郎; 河合功治; 矢下亜紀良; 阿部薫明; 吉田靖弘, 日本蛋白質科学会年会プログラム・要旨集, 15th, 139, 26 May 2015
  Japanese
• 骨芽細胞のマイクロ・ナノパターン化ゼラチンシートへの細胞付着性及び配向性
  加我 公行; 横山 敦郎; 吉田 靖弘, 日本補綴歯科学会誌, 7, 特別号, 249, 249, May 2015
  (公社)日本補綴歯科学会, Japanese
• 1液性ボンディング材中に含まれるシランカップリング材のカップリング効果の検討
  吉原 久美子; 長岡 紀幸; 入江 正郎; 西川 悟郎; 丸尾 幸憲; 松本 卓也; 皆木 省吾; 吉田 靖弘, 日本補綴歯科学会誌, 7, 特別号, 169, 169, May 2015
  (公社)日本補綴歯科学会, Japanese
• 1液性ボンディング材中に含まれるシランカップリング材のカップリング効果の検討
  吉原久美子; 長岡紀幸; 入江正郎; 西川悟郎; 丸尾幸憲; 松本卓也; 皆木省吾; 吉田靖弘, 日本補綴歯科学会誌(Web), 7, 2‐3‐2 (WEB ONLY), May 2015
  Japanese
• Analysis of self-assembled presipitate in bonding agent
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科理工学会誌, 34, 2, 169, 169, 25 Mar. 2015
  To chemically unravel the adhesion mechanism of GPDM with hydroxyapatite (HAp) and dentin. The molecular interaction of GPDM with HAp was analyzed using X-ray diffraction (XRD) and solid-state NMR (^<31>P MAS NMR, ^<31>P CP-MAS NMR, ^1H MAS NMR). XRD showed the formation of CaHPO_4・2H_2O (DCPD) on HAp, but only after 24hrs. NMR confirmed the adsorption of GPDM onto HAp, even after 5min. However, GPDM was removed from HAp after being washed with water, this in contrast with 10-MDP that remained stably adhered onto HAp. The bond of GPDM to HAp was less strong than that of 10-MDP., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Behavior of cell adhesion on micro/nano-patterned materials
  赤坂司; 加我公行; 阿部薫明; 吉田靖弘, 日本歯科理工学会誌, 34, 2, 58, 58, 25 Mar. 2015
  We investigated preparation of micro/nano-pattered composite resin and collagen micro-pillars with wings. And then the behaviors of cell adhesion were estimated using human periodontal ligament fibroblasts (HPDLF). As a result, alignment of cells against groove pattern was largely influenced by pre-coating of proteins such as fibronectin, BSA, and so on. And then the cells on collagen micro-pillar were aligned along wings. Furthermore ECM fibers produced by the cells were aligned along wings, too. These data supported that the micro/nano-pattern can control the alignment of cells and ECM fibers., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• 歯科から医科へ―歯質接着の基礎研究が生んだ医療イノベーション―
  吉田靖弘, 北海道歯学雑誌, 35, 2, 179, 179, 15 Mar. 2015
  北海道歯学会, Japanese
• 2P-047 Study of long-term stability of the enzyme in high soluble ionic liquids for the use to the biocatalyst stabilizing agent
  Kaneko Kotaro; Yashita Akira; Kawai Koji; Abe Shigeaki; Yoshida Yasuhiro, 日本生物工学会大会講演要旨集, 67, 186, 186, 2015
  日本生物工学会, Japanese
• モノマーの純度が接着とその耐久性に及ぼす影響
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本接着歯学会学術大会講演集(Web), 33rd, 3, P7 (WEB ONLY), 159, Dec. 2014
  (一社)日本接着歯学会, Japanese
• 2ケイ酸リチウムガラスセラミクスの構造とカップリング処理の評価
  長岡紀幸; 吉原久美子; 入江正郎; 吉田靖弘, 日本接着歯学会学術大会講演集(Web), 33rd, 3, KOEN3 (WEB ONLY), 144, Dec. 2014
  (一社)日本接着歯学会, Japanese
• リン酸化プルランを基材とした複合材料の力学物性
  沖原巧; 中尾行宏; 中井暉; 渡邉智也; 松尾健哉; 吉田靖弘, レオロジー討論会講演要旨集, 62nd, 174, 175, 15 Oct. 2014
  Japanese
• Effects of cell type on cell adhesion of micro/nano-patterned composite resin
  赤坂司; 加我公行; 阿部薫明; 吉田靖弘, 日本歯科理工学会誌, 33, 5, 466, 466, 20 Sep. 2014
  Patterned biomaterial is known to be constituent of enamel of teeth and periodontal membrane etc. Therefore we investigated preparation of micro/nano-pattered composite resin scaffolds and estimated cell adhesion using various types of cells. As a result of cell adhesion, the pattern resin indicated higher cell adhesion activity than plane resin. Furthermore, tendency of ratio of the attached cells using each cell was slightly different. In the case of osteoblasts, there was a small difference. These data supported that the ratio of the attached cells on the patterns was affected by type of cells., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Development of protein stock solution using ionic liquids: A long-term stability of proteins at room temperature
  阿部薫明; 金子恒太郎; 河合功治; 赤坂司; 吉田靖弘, 日本歯科理工学会誌, 33, 5, 446, 446, 20 Sep. 2014
  Ionic Liquids (ILs) are organic salts of liquid state at room temperature. They have been interested by their unique properties and much attention as useful solvents for electrochemical, enzymatic reaction and green chemistry. Recently, many researchers applied the ILs as protein stabilization solvents. Interestingly, some protein can be soluble, stable and remain activity in some ILs. This is potent to use ILs for stock solvent of proteins for pharmaceutical use. As conventional methods require optimizing stock conditions for each protein or leading to decrease bioactivity, improvement to effective methods is required. Therefore, we investigate stability of proteins in ILs., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Analysis of self-assembled presipitate in bonding agent
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科理工学会誌, 33, 5, 426, 426, 20 Sep. 2014
  Chemical interaction of specific functional monomers with hydroxyapatite (HAp) has been shown to contribute to bond durability. In previous research, some 10-MDP-based adhesives were found to produce 'nano-layering' at the interface with dentin. Here, we investigated the nano-layering potential of two phosphoric-acid monomer containing 1-step self-etch adhesives, G-aenial Bond/G-Bond Plus (GC). XRD disclosed nano-layering for the adhesives bothe on dentin and glass plate. TEM revealed nano-layering near the interface between adhesive and composite. STEM-EDS confirmed the self-assembled nano-layering from G-aenical Bond consisted of aluminum. The Self-assembled nano-layering might affect the mechanical property for adhesives., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Fabrication of micro/nano-patterned gelatin for cell adhesion studies
  加我公行; 赤坂司; 横山敦郎; 吉田靖弘, 日本歯科理工学会誌, 33, 5, 464, 464, 20 Sep. 2014
  In this study, we developed the micro/nano-patterned gelatin sheets to improve cell attachment of human gingival fibroblasts. The micro-and nano-patterned sheets with pattern shape (groove, hole, and pillar) and size (2μm, 1μm, and 0.5μm) were prepared from gelatin solution via nano-imprint method. As a result of cell attachment assay for lh-incubation, the attached cells on patterned-gelatin sheets were larger than those on plane-gelatin sheets. Further, both size and shape of the pattern greatly influenced the degree of extension of the attached cell., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Analysis of MTA cement and glass ionomer cement interface
  長岡紀幸; 吉原久美子; 吉田靖弘, 日本歯科理工学会誌, 33, 5, 486, 486, 20 Sep. 2014
  There was a few study that investigated the interface between MTA cement and glass ionomer cement. To analyze the interface we prepared cross section sample of the interface and observed it by a scanning electron microscope. The crystal growth due to the curing was observed in MTA cement near the interface. The matrix of glass ionomer cement infiltrated into the filler and crystal of MTA cement. The results suggested that the interface of MTA cement and glass ionomer cement showed good adhesion. Glass ionomer cement might be a good filling material for MTA cement treatment., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• リン酸化プルラン‐リン酸カルシウム複合体の力学物性の制御
  渡邉俊; 中井暉; 渡邉智也; 沖原巧; 吉田靖弘; 松尾健哉, 高分子学会予稿集(CD-ROM), 63, 2, ROMBUNNO.1PF094, 03 Sep. 2014
  Japanese
• リン酸化プルランを基材とした持続型口腔ケア剤の機能発現メカニズム
  沖原巧; 岡島裕樹; 亀ノ上翔吾; 難波尚子; 長岡紀幸; 高柴正悟; 吉田靖弘, 高分子学会予稿集(CD-ROM), 63, 2, ROMBUNNO.3Y08, 03 Sep. 2014
  Japanese
• リン酸化プルランと薬剤の複合構造と薬剤徐放性
  吉実伸悟; 沖原巧; 吉田靖弘; 松尾健哉, 高分子学会予稿集(CD-ROM), 63, 2, ROMBUNNO.1PF092, 03 Sep. 2014
  Japanese
• Phosphorylated gelatin to enhance cell adhesion to titanium
  Shin-Hye Park; Liping Zhu; Seiichi Tada; Sei Obuse; Yasuhiro Yoshida; Mariko Nakamura; Tae Il Son; Satoshi Tsuneda; Yoshihiro Ito, POLYMER INTERNATIONAL, 63, 9, 1616, 1619, Sep. 2014
  English
• 新しい可視光硬化ゼラチンを用いた家兎骨軟骨欠損モデルにおける組織修復効果
  馬崎哲朗; 山根健太郎; 吉田晶; 松川昭博; 中村真理子; 吉田靖弘; 北嶋隆; 伊藤嘉浩; 尾崎敏文, 日本整形外科学会雑誌, 88, 8, S1587, S1587, 29 Aug. 2014
  (公社)日本整形外科学会, Japanese
• 新規生体材料によるインプラントコーティング技術
  香川洋平; 馬崎哲朗; 山根健太郎; 篠原健介; 吉田晶; 松川昭博; 中村真理子; 吉田靖弘; 北嶋隆; 伊藤嘉浩; 尾崎敏文, 日本整形外科学会雑誌, 88, 8, S1501, S1501, 29 Aug. 2014
  (公社)日本整形外科学会, Japanese
• QCM‐Dによる様々な細胞種の接着と伸展の観察
  竹下裕治; 張本乾一; 玉井美保; 南隆之; 荻博次; 長岡紀幸; 松川昭博; 吉田靖弘; 田川陽一, 日本生物工学会大会講演要旨集, 66th, 154, 154, 05 Aug. 2014
  日本生物工学会, Japanese
• Simple and Rapid Conductive Preparation of Wet Biological Samples for SEM Observation: Use of an Asymmetrical Choline-like Room Temperature Ionic Liquid as a Visualizing Agent
  ABE SHIGEAKI; HYONO ATSUSHI; KAWAI KOJI; NAKAMURA KOICHI; YAWAKA YASUTAKA; YOSHIDA YASUHIRO; YONEZAWA TETSU, Nano Biomed, 6, 1, 41, 46, 30 Jun. 2014
  English
• リン酸化プルランとリン酸カルシウム複合体の物性発現メカニズム
  渡邉俊; 中井暉; 中尾行広; 沖原巧; 吉田靖弘; 松尾健哉, 高分子学会予稿集(CD-ROM), 63, 1, ROMBUNNO.1PH142, 09 May 2014
  Japanese
• 薬剤含有リン酸化プルラン人工骨と薬剤徐放性
  吉実伸悟; 沖原巧; 吉田靖弘; 松尾健哉, 高分子学会予稿集(CD-ROM), 63, 1, ROMBUNNO.3PB126, 09 May 2014
  Japanese
• 家兎骨軟骨欠損モデルにおける可視光硬化ゼラチンおよび骨髄間質細胞の組織修復効果
  馬崎 哲朗; 塩崎 泰之; 山根 健太郎; 吉田 晶; 松川 昭博; 中村 真理子; 吉田 靖弘; 北嶋 隆; 伊藤 嘉浩; 尾崎 敏文, 移植, 49, 1, 178, 178, May 2014
  (一社)日本移植学会, Japanese
• 可視光硬化型ゼラチンとコラーゲン結合性肝細胞増殖因子を用いた脊髄損傷治療
  山根健太郎; 馬崎哲朗; 篠原健介; 吉田晶; 松川昭博; 中村真理子; 吉田靖弘; 北嶋隆; 伊藤嘉浩; 田中雅人; 尾崎敏文, J Spine Res, 5, 3, 473, 473, 25 Mar. 2014
  (一社)日本脊椎脊髄病学会, Japanese
• The effect of functional monomers on long-term bond durability
  吉原久美子; 長岡紀幸; 吉田靖弘, 日本歯科理工学会誌, 33, 2, 142, 142, 25 Mar. 2014
  Our previous studies reported that not only the phosphoric-acid group but also the spacer and group and it length affect the chemical interaction potential with hydroxyapatite (HAp) and dentin. The objective of this study was to evaluate the microtensile bond strength (μTBS) after 50,000 and 100,000 thermal cycles to dentin of experimental adhesives containing 5 different phosphate functional monomers and contact angle of these monomer treated dentin and HAp. The μTBS of MF8P and MF8P/10-MDP did not decrease significantly after 100,000 cycles. However, the μTBS of 2-MEP, 4-MET, 6-MHP and 10-MDP decreased. The interfacial ultrastructure was showed the aggressive etching effect on 10-MDP. MF8P and10-MDP showed high contact angle. MF8P showed good bonding durability. Etching efficacy and hydrophobic surface of these monomers affect the long-term bond durability., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• 多糖誘導体リン酸化プルランをキーマテリアルとした多目的硬組織接着剤の開発
  吉田 靖弘; 松川 昭博; 高柴 正悟; 沖原 巧; 伊東 孝, 医科学応用研究財団研究報告, 31, 23, 27, Feb. 2014
  (公財)鈴木謙三記念医科学応用研究財団, Japanese
• 多糖誘導体リン酸化プルランをキーマテリアルとした多目的硬組織接着剤の開発
  吉田靖弘; 松川昭博; 高柴正悟; 沖原巧; 伊東孝, 医科学応用研究財団研究報告(CD-ROM), 31, 23, 27, Feb. 2014
  Japanese
• 家兎骨軟骨欠損モデルにおける可視光硬化ゼラチンおよび骨髄間質細胞の組織修復効果
  馬崎哲朗; 塩崎泰之; 山根健太郎; 吉田晶; 松川昭博; 中村真理子; 吉田靖弘; 北嶋隆; 伊藤嘉浩; 尾崎敏文, 移植(Web), 49, 1, 178(J-STAGE), 2014
  Japanese
• 直交配置型FIB-SEMを用いた歯質接着界面の3次元観察
  長岡 紀幸; 吉原 久美子; 吉田 靖弘; 鳥井 康弘, 接着歯学, 31, 3, 120, 120, Nov. 2013
  (一社)日本接着歯学会, Japanese
• P-20 Analysis of the zirconia surface treated with phosphoric acid monomers
  Nagaoka N; Tamada Y; Irie M; Yoshihara K; Yoshida Y; Nishigawa G; Maruo Y; Matsumoto T; Minagi S; Hayakawa S, The journal of the Japanese Society for Dental Materials and Devices, 32, 5, 354, 354, Sep. 2013
  Adhesion of inorganic material requires surface coupling processing. The coupling of zirconia with a phosphoric acid monomer is effective. In the present study, the relation between concentration of the phosphoric acid monomer solution used for zirconia surface treatment, and adhesion strength was investigated. The chemical bond state of the phosphate acid monomer coupled to the zirconia surface was analyzed using NMR as well. It was suggested that the nature of the chemical bond between zirconia and phosphate acid monomer was ionic., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• 家兎骨軟骨欠損モデルにおける可視光硬化ゼラチンおよび骨髄間質細胞の組織修復効果
  香川 洋平; 馬崎 哲朗; 塩崎 泰之; 山根 健太郎; 吉田 晶; 松川 昭博; 中村 真理子; 吉田 靖弘; 北嶋 隆; 伊藤 嘉浩; 尾崎 敏文, 日本整形外科学会雑誌, 87, 8, S1405, S1405, Aug. 2013
  (公社)日本整形外科学会, Japanese
• 可視光硬化ゼラチンとcollagen binding domain(CBD)-BMP4の併用による家兎骨軟骨組織修復効果
  馬崎 哲朗; 塩崎 泰之; 山根 健太郎; 香川 洋平; 篠原 健介; 吉田 晶; 松川 昭博; 中村 真理子; 吉田 靖弘; 北嶋 隆; 伊藤 嘉浩; 尾崎 敏文, 日本整形外科学会雑誌, 87, 8, S1606, S1606, Aug. 2013
  (公社)日本整形外科学会, Japanese
• 【歯質に対する接着耐久性を考える】 アパタイトとの化学的相互作用
  吉田 靖弘; 吉原 久美子; 井上 哲, 日本歯科理工学会誌, 32, 4, 257, 260, Jul. 2013
  (一社)日本歯科理工学会, Japanese
• TEM microstructure analysis of surface treated zirconia by rocatec system
  TAMADA Y; NAGAOKA N; TAKETA H; YOSHIHARA K; IRIE M; NISHIGAWA G; MARUO Y; YOSHIDA Y; TORII Y; MATSUMOTO T; MINAGI S, The journal of the Japanese Society for Dental Materials and Devices, 32, 2, 152, 152, Mar. 2013
  Rocatec treatment can make strong share bond strength by making rough surface. It is reported that to make close silane coupling layer on zirconia surface is difficult than making on silica. Making close silane coupling layer on zirconia surface is very important to gain strong share bond strength. The aim of this study is to evaluate detailed structure of silane coupling layer on zirconia surface by exanimating SEM and STEM images. Sedimentary layer of globular silica is observed on zirconia surface. Remaining globular silica has a bud effect on share bond strength. It is important to remove Rocatec sand., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• リン酸化多糖-界面活性剤複合体の物性と機能
  沖原巧; 亀ノ上翔吾; 吉田靖弘; 難波尚子; 高柴正悟; 長岡紀幸, 高分子学会予稿集(CD-ROM), 62, 2, 2013
• リン酸化多糖と界面活性剤との複合体による持続的作用を持つ口腔用抗菌剤の開発
  沖原巧; 亀ノ上翔吾; 難波尚子; 吉田靖弘; 長岡紀幸; 高柴正悟, セルロース学会年次大会講演要旨集, 20th, 2013
• コラーゲン結合性骨形成蛋白質BMP4の骨芽細胞に対する影響
  吉田 晶; 古松 毅之; 塩崎 泰之; 馬崎 哲朗; 松川 昭博; 中村 真理子; 吉田 靖弘; 北嶋 隆; 伊藤 嘉浩; 尾崎 敏文, 移植, 47, 6, 498, 498, Dec. 2012
  (一社)日本移植学会, Japanese
• 天然多糖プルランのリン酸化合物と塩化セチルピリジニウム混合液の細胞と生体および口腔内細菌へ与える影響
  伊東 孝; 河田 有祐; 難波 尚子; 吉田 靖弘; 前田 博史; 高柴 正悟, 岡山歯学会雑誌, 31, 2, 92, 92, Dec. 2012
  岡山歯学会, Japanese
• 炭酸含有アパタイトナノ粒子の作製および水晶基板への電気泳動コーティング
  横浜 優人; 川下 将一; 石川 邦夫; 吉田 靖弘, 日本バイオマテリアル学会大会予稿集, シンポジウム2012, 475, 475, Nov. 2012
  日本バイオマテリアル学会, Japanese
• マウスを用いた遺伝子改変成長因子Collagen binding domain BMP4の効果
  塩崎 泰之; 馬崎 哲郎; 山根 健太郎; 吉田 靖弘; 中村 真理子; 北嶋 隆; 伊藤 嘉浩; 尾崎 敏文; 松川 昭博, 日本バイオマテリアル学会大会予稿集, シンポジウム2012, 269, 269, Nov. 2012
  日本バイオマテリアル学会, Japanese
• ウサギの骨欠損モデルにおいてコラーゲン結合性骨形成蛋白質は骨形成を促進する
  吉田 晶; 塩崎 泰之; 馬崎 哲朗; 古松 毅之; 松川 昭博; 中村 真理子; 吉田 靖弘; 北島 隆; 伊藤 嘉浩; 尾崎 敏文, 日本バイオマテリアル学会大会予稿集, シンポジウム2012, 270, 270, Nov. 2012
  日本バイオマテリアル学会, Japanese
• 家兎骨軟骨欠損モデルにおける可視光硬化ゼラチンの骨軟骨組織修復の効果
  馬崎 哲朗; 塩崎 泰之; 吉田 晶; 松川 昭博; 中村 真理子; 吉田 靖弘; 北嶋 隆; 伊藤 嘉浩; 尾崎 敏文, 日本バイオマテリアル学会大会予稿集, シンポジウム2012, 325, 325, Nov. 2012
  日本バイオマテリアル学会, Japanese
• 汚染されたジルコニア表面に対するリン酸エステルによるカップリング処理
  長岡 紀幸; 玉田 宜之; 入江 正郎; 西川 悟郎; 丸尾 幸憲; 吉田 靖弘; 松本 卓也, 接着歯学, 30, 3, 114, 114, Nov. 2012
  (一社)日本接着歯学会, Japanese
• Nano-Interfacial Analysis for Tooth Bonding
  YOSHIDA Yasuhiro, Annals of Japan Prosthodontic Society, 4, 4, 353, 363, 10 Oct. 2012
  Dental adhesives have rapidly improved since they were introduced more than 50 years ago. Decayed or fractured teeth can be reconstructed minimally, invasively, and nearly invisibly using resin composites. The practice of fixed prosthodontics has changed dramatically with the introduction of innovative adhesive luting agents. Although the bonding mechanism of dental adhesives to tooth tissues is complicated, the fundamental mechanism is essentially based on an exchange process, in which minerals removed from the dental hard tissues are replaced by resin monomers that upon polymerization become micro-mechanically interlocked in the created porosities. In addition to micro-mechanical interlocking through hybridization, the potential benefits of additional chemical interactions between functional monomers/polymers and tooth substrate components have recently regained attention. In this review paper, we focus on the tooth bonding mechanism, especially on how chemical interaction at the biomaterial-hard tissue interface can improve bond durability., Japan Prosthodontic Society, Japanese
• Adsorption characteristics of phosphoric acid ester monomer for zirconia surface
  TAMADA Y; NAGAOKA N; HAYAKAWA S; IRIE M; NISHIGAWA G; MARUO Y; YOSHIDA Y; MATSUMOTO T; OSAKA A; MINAGI S, The journal of the Japanese Society for Dental Materials and Devices, 31, 5, 479, 479, Sep. 2012
  Copings and frameworks for all-ceramic restorations can be made out of zirconia satisfying the increasing demand for esthetic restorations. The organification on zirconia surface is the most important thing to improve adhesive strength. Priming treatment such as phosphoric acid ester monomer or silane coupling agent is necessary to strong bond strength on zirconia. Many primers on market include phosphoric acid ester monomer and silane coupling agent. Using this primer, phosphoric acid ester monomer is mainly adsorbed on zirconia. The aim of this study is to evaluate the Adsorption characteristics of phosphoric acid ester monomer for zirconia surface., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Osteoblasts defeat fibroblasts on titanium surface during osteointegration steps
  K. Harimoto; Y. Yoshida; K. Yoshihara; N. Nagaoka; B. Van Meerbeek; Y. Tagawa, JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 6, 236, 236, Sep. 2012
  English, Summary international conference
• ウサギの骨欠損モデルにおけるコラーゲン結合性骨形成蛋白質の有効性
  馬崎 哲朗; 塩崎 泰之; 吉田 晶; 松川 昭博; 中村 真理子; 吉田 靖弘; 北嶋 隆; 伊藤 嘉浩; 尾崎 敏文, 日本整形外科学会雑誌, 86, 8, S1071, S1071, Aug. 2012
  (公社)日本整形外科学会, Japanese
• コラーゲン結合型BMP4の骨芽細胞に対する影響
  吉田 晶; 馬崎 哲朗; 塩崎 泰之; 古松 毅之; 松川 昭博; 中村 真理子; 吉田 靖弘; 北嶋 隆; 伊藤 嘉浩; 尾崎 敏文, 日本整形外科学会雑誌, 86, 8, S1343, S1343, Aug. 2012
  (公社)日本整形外科学会, Japanese
• 低濃度CPCと新基質リン酸プルランによる口腔バイオフィルムの抑制
  河田 有祐; 難波 尚子; 伊東 孝; 吉田 靖弘; 前田 博史; 高柴 正悟, 日本歯科医師会雑誌, 65, 5, 637, 637, Aug. 2012
  (公社)日本歯科医師会, Japanese
• How Can Biomaterials Contribute to Health Care for Elderly People?
  YOSHIDA Yasuhiro, Ronen Shika Igaku, 26, 4, 387, 393, 31 Mar. 2012
  標記について、歯科材料、特に歯質接着性材料を中心に解説した。歯質接着性材料は、レジン系とグラスアイオノマー系が多用されている。レジン系では歯質のエッチング処理後に水洗が必要なトータルエッチングシステムと、エッチングとプライミングを同時に行うため水洗が不要なセルフエッチングシステム、さらにグラスアイオノマーセメントについて概説した。また、新しい生体硬組織用の接着材料である多糖誘導体リン酸化プルランについて述べた。, Japanese Society of Gerodontology, Japanese
• ジルコニア表面のリン酸モノマーによる有機化の検討
  玉田 宜之; 長岡 紀幸; 早川 聡; 入江 正郎; 西川 悟郎; 丸尾 幸憲; 吉田 靖弘; 松本 卓也; 尾坂 明義; 皆木 省吾, 日本歯科理工学会誌, 31, 2, 190, 190, Mar. 2012
  Copings and frameworks for all-ceramic restorations can be made out of zirconia satisfying the increasing demand for esthetic restorations. The organification on zirconia surface is the most important to strengthen bond strength. The aim of this study is to evaluate the relationship sher-bond strength and influence of cleaning and organification of contaminated zirconia. Zirconia disc was contaminated by saliva. Cleaning and organification was performed in different six ways. In some ways we used silica. Using silica is effective to clean saliva on zirconia surface., (一社)日本歯科理工学会, Japanese
• リン酸化多糖-界面活性剤複合体の機能と物性
  亀ノ上翔吾; 沖原巧; 難波尚子; 吉田靖弘; 高柴正悟; 長岡紀幸, 高分子学会予稿集(CD-ROM), 61, 1, 2012
• リン酸化プルランの合成と口腔用持続的抗菌剤の開発
  亀ノ上翔吾; 沖原巧; 吉田靖弘; 難波尚子; 高柴正悟; 長岡紀幸, 高分子学会予稿集(CD-ROM), 61, 2, 2012
• リン酸化プルランを用いた持続作用を持つ口腔用抗菌剤の開発
  沖原巧; 亀ノ上翔吾; 難波尚子; 吉田靖弘; 長岡紀幸; 高柴正悟, セルロース学会年次大会講演要旨集, 19th, 2012
• 歯科領域におけるInterface制御 歯質接着の解析と制御
  吉田 靖弘, 日本バイオマテリアル学会大会予稿集, 33回, 201, 201, Nov. 2011
  日本バイオマテリアル学会, Japanese
• 天然多糖プルランのリン酸化合物と塩化セチルピリジニウム混合液のラットへの反復経口投与毒性試験と細胞へ与える影響
  河田 有祐; 難波 尚子; 峯柴 史; 吉田 靖弘; 前田 博史; 高柴 正悟, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 135回, 210, 210, Sep. 2011
  (NPO)日本歯科保存学会, Japanese
• 硬組織接着性多糖誘導体リン酸化プルランによる薬剤徐放能の解析
  馬崎 哲朗; 塩崎 泰之; 高畑 智宏; 吉田 靖弘; 杉本 佳久; 三澤 治夫; 尾崎 修平; 田中 雅人; 尾崎 敏文, 日本整形外科学会雑誌, 85, 8, S1303, S1303, Aug. 2011
  (公社)日本整形外科学会, Japanese
• 生体材料は高齢者の健康維持にどのように貢献できるか
  吉田 靖弘, 日本老年歯科医学会総会・学術大会プログラム・抄録集, 22回, 62, 62, Jun. 2011
  (一社)日本老年歯科医学会, Japanese
• 岡山県の介護施設における口腔ケアに対する意識とニーズ
  河田 有祐; 難波 尚子; 伊東 孝; 吉田 靖弘; 前田 博史; 鈴木 一臣; 高柴 正悟, 岡山歯学会雑誌, 30, 1, 36, 37, Jun. 2011
  岡山歯学会, Japanese
• フラボノイドによる歯髄炎症制御の応用 リン酸化プルラン複合体を用いての検討(第2報)
  米廣 純子; 山下 明子; 吉田 靖弘; 西村 英紀, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 134回, 8, 8, May 2011
  (NPO)日本歯科保存学会, Japanese
• セルフエッチングシステムを用いる前のエナメルエッチングは有効か ナノスケール界面観察によるnano-interactionとresin-smear complexの明示化
  峯 篤史; De Munck Jan; 窪木 拓男; 吉田 靖弘; 鈴木 一臣; Van Meerbeek Bart, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 134回, 66, 66, May 2011
  (NPO)日本歯科保存学会, Japanese
• 硬組織接着性多糖誘導体リン酸化プルランの開発
  高畑 智宏; 塩崎 泰之; 吉田 靖弘; 三澤 治夫; 杉本 佳久; 田中 雅人; 尾崎 敏文, Journal of Spine Research, 2, 3, 647, 647, Mar. 2011
  (一社)日本脊椎脊髄病学会, Japanese
• 硬組織接着性多糖誘導体リン酸化プルランの開発
  高畑 智宏; 塩崎 泰之; 吉田 靖弘; 杉本 佳久; 三澤 治夫; 吉田 晶; 田中 雅人; 尾崎 敏文, 日本整形外科学会雑誌, 85, 2, S108, S108, Feb. 2011
  (公社)日本整形外科学会, Japanese
• 硬組織接着性多糖誘導体リン酸化プルランによる薬剤徐放能の検討
  塩崎 泰之; 高畑 智宏; 吉田 靖弘; 杉本 佳久; 三澤 治夫; 吉田 晶; 田中 雅人; 尾崎 敏文, 日本整形外科学会雑誌, 85, 2, S109, S109, Feb. 2011
  (公社)日本整形外科学会, Japanese
• 高分解能走査電子顕微鏡によるインプラント/生体組織界面の観察
  白井肇; 武田宏明; 長岡紀幸; 吉田靖弘; 坂本隼一; 原哲也; 河野隆幸; 鈴木康司; 皆木省吾; 鈴木一臣; 鳥井康弘, 岡山歯学会雑誌, 30, 1, 39, 40, 2011
  岡山歯学会, Japanese
• リン酸化プルラン-界面活性剤複合体の構造と物性
  亀ノ上翔吾; 山本大樹; 沖原巧; 吉田靖弘; 難波尚子; 長岡紀幸; 高柴正悟, 高分子学会予稿集(CD-ROM), 60, 2 Disk1, 2011
• リン酸化多糖と低分子化合物の複合体形成
  沖原巧; 山本大樹; 吉田靖弘; 難波尚子; 長岡紀幸; 高島征助; 鈴木一臣; 高柴正悟, 高分子学会予稿集(CD-ROM), 60, 1 Disk1, 2011
• オッセオインテグレーションにおける線維芽細胞の影響
  張本乾一; 長岡紀幸; 鈴木一臣; 吉田靖弘; 田川陽一, 日本バイオマテリアル学会大会予稿集, 33rd, 259, 259, 2011
  日本バイオマテリアル学会, Japanese
• リン酸化多糖-薬剤分子複合体の構造と徐放物性
  辻昇吾; JO Yung Woo; 沖原巧; 吉田靖弘; 長岡紀幸, 高分子学会予稿集(CD-ROM), 60, 2 Disk1, 2011
• Measuring bond strength by conventional tensile or shear test
  YOSHIDA Yasuhiro; OKIHARA Takumi; NAGAOKA Noriyuki; INOUE Satoshi; TORII Yasuhiro, Adhesive Dentistry, 29, 2, 90, 94, 2011
  Japan Society for Adhesive Dentistry, Japanese
• 可視光硬化型ゼラチン誘導体の調製とその性能評価
  伊藤 嘉浩; 小布施 聖; 北嶋 隆; 水越 清美; 松江 登久; 田代 英夫; 中村 真理子; 吉田 靖弘, 日本バイオマテリアル学会大会予稿集, 32回, 151, 151, Nov. 2010
  日本バイオマテリアル学会, Japanese
• 機能性修復材料開発戦略のベクトルを探る 接着技術の応用による医用材料の高機能化
  吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 133回, 11, 11, Oct. 2010
  (NPO)日本歯科保存学会, Japanese
• Streptococcus mutansのバイオフィルム形成に対するレクチンによる抑制機構
  伊東 孝; 吉田 靖弘; 峯柴 淳二; 難波 尚子; 今村 幸治; 竹内 英明; 鈴木 一臣; 高柴 正悟, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 133回, 40, 40, Oct. 2010
  (NPO)日本歯科保存学会, Japanese
• 岡山県の介護施設における口腔ケアのアンケート調査
  難波 尚子; 河田 有祐; 伊東 孝; 吉田 靖弘; 前田 博史; 鈴木 一臣; 高柴 正悟, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 133回, 204, 204, Oct. 2010
  (NPO)日本歯科保存学会, Japanese
• Drug delivery by sensing its concentration
  YOSHIDA Y; NAMBA N; NAGAOKA N; NAKAMURA M; TAKASHIBA S; SUZUKI K, The journal of the Japanese Society for Dental Materials and Devices, 29, 5, 452, 452, 20 Sep. 2010
  Carrier materials with sensor for drug concentration hold great promise as smart delivery systems for drugs which have strong side effects, such as anticancer agents and bactericides. However, any carriers sensing drug concentration have not been turned to practical use. We found that the phosphopullulan and CPC complex changed their interaction at critical micelle concentration of CPC with phosphopullulan. It was also revealed that this phenomenon strongly related the bactericidal effects of the complex., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• フラボノイドによる歯髄炎症制御の応用 リン酸化プルランセメントを用いての検討
  米廣 純子; 山下 明子; 吉田 靖弘; 西村 英紀, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 132回, 204, 204, May 2010
  (NPO)日本歯科保存学会, Japanese
• Application of chemical analyses in dental adhesive technology
  YOSHIDA Yasuhiro; NAKAYAMA Youichi; YOSHIHARA Kumiko; HANABUSA Masao; MINE Atsushi; INOUE Satoshi; SUZUKI Kazuomi; VAN MEERBEEK Bart, 接着歯学, 28, 1, 1, 13, Apr. 2010
  (一社)日本接着歯学会, Japanese
• 細胞の接着性が骨形成に及ぼすバイオマテリアルの影響
  張本乾一; 長岡紀幸; 赤池敏宏; 鈴木一臣; 吉田靖弘; 田川陽一; 田川陽一; 田川陽一, 再生医療, 9, 2010
• リン酸化プルラン-界面活性剤複合体の形成と物性
  山本大樹; 沖原巧; 吉田靖弘; 難波尚子; 長岡紀幸; 高島征助; 鈴木一臣; 高柴正悟, 高分子学会予稿集(CD-ROM), 59, 1 Disk1, 2010
• リン酸化プルランを利用した持続的効果を有する口腔用抗菌剤
  山本大樹; 沖原巧; 吉田靖弘; 難波尚子; 長岡紀幸; 高島征助; 鈴木一臣; 高柴正悟, 高分子学会予稿集(CD-ROM), 59, 2 Disk1, 2010
• リン酸化多糖-界面活性剤複合体の機能と構造
  山本大樹; 沖原巧; 吉田靖弘; 難波尚子; 長岡紀幸; 高島征助; 鈴木一臣; 高柴正悟, 高分子学会予稿集(CD-ROM), 59, 2 Disk1, 2010
• バイオマテリアルに対する細胞の接着性がオッセオインテグレーションに及ぼす影響
  張本乾一; 長岡紀幸; 赤池敏宏; 鈴木一臣; 吉田靖弘; 田川陽一, 日本バイオマテリアル学会大会予稿集, 32nd, 208, 208, 2010
  日本バイオマテリアル学会, Japanese
• リン酸化多糖と界面活性剤の複合体の構造と物性
  山本大樹; 沖原巧; 吉田靖弘; 難波尚子; 長岡紀幸; 高島征助; 鈴木一臣; 高柴正悟, 日本化学会西日本大会講演要旨集, 2010, 2010
• リン酸化多糖を担体とする口腔用抗菌剤の開発
  山本大樹; 沖原巧; 吉田靖弘; 難波尚子; 長岡紀幸; 高島征助; 鈴木一臣; 高柴正悟, 日本化学会西日本大会講演要旨集, 2010, 2010
• 口腔用抗菌剤担体としてのリン酸化多糖の合成と作用機構の解明
  山本大樹; 沖原巧; 吉田靖弘; 難波尚子; 長岡紀幸; 高島征助; 鈴木一臣; 高柴正悟, 高分子学会予稿集(CD-ROM), 59, 1 Disk1, 2010
• Enamel-smear compromises bonding by mild self-etch adhesives
  Mine A; De Munck J; Cardoso MV,Van; Landuyt KL; Poitevin A; Kuboki T; Yoshida Y; Suzuki K; Van Meerbeek B, J Dent Res., 2010
• The quasi-three-dimensional marginal leakage of full-coverage crowns : resin coating versus sodium hypochlorite treatment
  KAWAHARA D, Int J Prosthodont, 23, 406, 408, 2010
• TEM characterization of a silorane composite bonded to enamel/dentin.
  Atsushi Mine; Jan De Munck; Annelies Van Ende; Marcio Vivan Cardoso; Takuo Kuboki; Yasuhiro Yoshida; Bart Van Meerbeek, Dental materials : official publication of the Academy of Dental Materials, 26, 6, 524, 32, 2010, [International Magazine]
  OBJECTIVES: The low-shrinking composite composed of combined siloxane-oxirane technology (Filtek Silorane, 3M ESPE, Seefeld, Germany) required the development of a specific adhesive (Silorane System Adhesive, 3M ESPE), in particular because of the high hydrophobicity of the silorane composite. The purpose of this study was to characterize the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). METHODS: Non-demineralized/demineralized 70-90 nm sections were prepared following common TEM specimen processing procedures. RESULTS: TEM revealed a typical twofold build-up of the adhesive resin, resulting in a total adhesive layer thickness of 10-20 microm. At bur-cut enamel, a tight interface without distinct dissolution of hydroxyapatite was observed. At bur-cut dentin, a relatively thin hybrid layer of maximum a few hundreds of nanometer was formed without clear surface demineralization. No clear resin tags were formed. At fractured dentin, the interaction appeared very superficial (100-200 nm). Distinct resin tags were formed due to the absence of smear plugs. Silver-nitrate infiltration showed a varying pattern of both spot- and cluster-like appearance of nano-leakage. Traces of Ag were typically detected along some part of the enamel-adhesive interface and/or between the two adhesive resin layers. Substantially more Ag-infiltration was observed along the dentin-adhesive interface of bur-cut dentin, as compared to that of fractured dentin. CONCLUSIONS: The nano-interaction of Silorane System Adhesive should be attributed to its relatively high pH of 2.7. The obtained tight interface at both enamel and dentin indicates that the two-step self-etch adhesive effectively bridged the hydrophilic tooth substrate with the hydrophobic silorane composite., English
• 水晶基板へのアパタイトナノコーティング
  谷内 光史; 川下 将一; 石川 邦夫; 吉田 靖弘, 日本バイオマテリアル学会大会予稿集, 31回, 434, 434, Nov. 2009
  日本バイオマテリアル学会, Japanese
• 表面・界面研究が明日の歯科医療を創る
  吉田 靖弘; 鈴木 一臣, 日本歯科評論, 69, 10, 39, 40, Oct. 2009
  (株)ヒョーロン・パブリッシャーズ, Japanese
• Bactericide delivery system containing the phosphorylated polysaccharide as carrier material
  YOSHIDA Y; NAMBA N; NAGAOKA N; NAKAMURA M; TAKASHIBA S; SUZUKI K, Journal of the Japanese Society for Dental Materials and Devices, 28, 5, 296, 296, 10 Sep. 2009
  To effectively exploit the properties of antibacterial agents, we synthesized phosphorylated polysaccharides as the carrier for bactericide delivery to surface of apatitic substrates. It was indicated that 0.01% phosphorylated pullulan and 0.01% cetylpyridinium chloride (CPC) consistently exhibited an antibacterial effect, even after rinsing with distilled water, although CPC at a concentration of 10% showed no effect on bacterial growth after slight rinsing., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Antibacterial Coating for Prevention of Caries and Periodontal Disease
  YOSHIDA Yasuhiro, 日本歯科医学会誌, 28, 28, 39, 43, Mar. 2009
  日本歯科医学会, Japanese
• The formation mechanism of the biomaterial-tooth interface by functional phosphoric asid ester monomers
  Yoshihara Kumiko; Hayakawa Satoshi; Nagaoka Noriyuki; Yoshida Yasuhiro; Suzuki Kazuomi; Minagi Shogo, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 28, 2, 51, 51, Mar. 2009
  We examined the chemical interaction of functional phosphoric asid monomers (10-MDP, phenyl-P) and a hydroxyapatite (HAp) particle used for XRD and solid-state NMR. The monomer 10-MDP, which has better long-term durability, formed the layered structure of MDP-Ca salt on the HAp surface in early period. On the contrary, when phenyl-P interacted with HAp, DCPD was formed by decalcification of HAp but phenyl-P-Ca salt was not formed., The Japanese Society for Dental Materials and Devices, Japanese
• Effect of Smear Layer on Short/Long-term Bonding Effectiveness of One-step Adhesive Systems Bonded to Dentin
  FUKUOKA ANRI; KOSHIRO KEN'ICHI; INOUE SATOSHI; YOSHIDA YASUHIRO; TANAKA TOORU; IKEDA TAKATSUMI; SUZUKI KAZUOMI; KOMATSU HISANORI; SANO HIDEHIKO, 日本歯科保存学雑誌, 52, 1, 27, 38, Feb. 2009, [Peer-reviewed]
  The objective of this study was to investigate the ultra-morphological smear layers produced by various dental instruments, and to evaluate the effect of the smear layers on the short-and long-term bonding effectiveness of two commercially available one-step adhesive systems bonded to dentin. Smear layers were produced on flattened mid-coronal dentin surfaces of extracted human molars by one of the following materials: two types of diamond burs (regular-grit or superfine-grit) or two types of SiC papers (#600 or #2,000). Thickness of the smear layers and size of the particles of the four materials were measured by SEM. TEM images of the smear layers were taken, as well. Two types of dentin surfaces produced using a regular-grit diamond bur and #2,000 SiC paper were conditioned with either Clearfil S³Bond (Kuraray, S3) or Absolute (Dentsply-Sankin, AB) without light-curing, rinsed with acetone, and observed by SEM. Moreover, resin composites (Z-100, 3M ESPE) were bonded with either S3 or AB, and incrementally built-up on two types of the dentin surfaces. The specimens were sectioned into slabs and further trimmed into an hourglass shape with an interface area of approximately 1mm². They were left untouched or thermo-cycled (100,000 cycles). Micro-tensile bond strength (μTBS) testing and ultra-morphological observation by TEM were performed. The larger the particle sizes were, the thicker the width of the smear layer became, although TEM images of smear layers produced by the four materials revealed no differences in ultra-morphology. For S3, thick smear layers were not removed completely, whereas AB removed the smear layers despite their thickness. Whether the specimens were thermo-cycled or not, the μTBS of S3 on the dentin surface with thick smear layers was significantly lower than that of the thin, smear layer covered dentin surface. However, the μTBS of AB was not affected by the thickness of smear layers. For both adhesives, 100,000 thermo-cycles significantly decreased μTBS despite the thickness of the smear layers, and TEM images revealed scattered voids, which appears to be an indication of interfacial degradation. It is concluded that 1) the thickness of the smear layer produced on the dentin surface was dependent on the materials used; 2) thick smear layers affected the bonding effectiveness of S3 which had a low ability to dissolve the smear layers; 3) smear layers did not always affect the bonding effectiveness because AB completely removed the smear layers regardless of their thickness; and 4) the bonding effectiveness of one-step adhesive systems decreased after long-term thermo-cycling., The Japanese Society of Conservative Dentistry, Japanese
• 最新の接着技術を駆使した「G-ボンドプラス」の接着メカニズムと性能.
  鈴木 一臣; 吉田 靖弘, GC CIRCLE No.130, 26, 29, 2009
• 疑問に答えて:著者から読者へ(DE No.165, 166):歯質接着界面のナノスケールにおける解析
  吉田 靖弘, The Journal of Dental Engineering, 169, 38, 39, 2009
• ポリリン酸処理を用いたチタンインプラントの高機能化.
  前川賢治; 吉田靖弘; 下野賢吾; 大島正充; 峯 篤史; 鈴木一臣; 窪木拓男, 歯界展望, 61, 特別, 512, 512, 2009
  (公社)日本歯科医師会, Japanese
• Bonding effectiveness of two contemporary self-etch adhesives to enamel and dentin.
  Atsushi Mine; Jan De Munck; Marcio V Cardoso; Kirsten L Van Landuyt; André Poitevin; Takuo Kuboki; Yasuhiro Yoshida; Kazuomi Suzuki; Paul Lambrechts; Bart Van Meerbeek, Journal of dentistry, 37, 11, 872, 883, 2009, [International Magazine]
  OBJECTIVES: Among contemporary adhesives, self-etch adhesives have been adopted by general practitioners for routine adhesive restorative purposes, mainly because of their ease of use. However, many versions that differ for their clinical application procedure, pH, number of components, etc., are currently available on the market. The purpose of this study was to determine the bonding effectiveness of two new self-etch adhesives (Adper Easy Bond and Adper ScotchBond SE, 3M ESPE) to enamel and dentin using a micro-tensile bond strength (microTBS) protocol and to characterise the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). METHODS: The adhesives were applied onto coronal human enamel and dentin surfaces and built up with the micro-hybrid resin composite Z100 (3M ESPE). The 'gold-standard' two-step self-etch adhesive Clearfil SE Bond (Kuraray) served as control. Specimens were sectioned to sticks and trimmed at the interface to a cylindrical hour-glass shape ('trimmed' micro-specimens). Non-demineralized and demineralized TEM sections through the adhesive-dentin/enamel interface were prepared by ultra-microtomy. RESULTS: The microTBS of the two self-etch adhesives to enamel was statistically significantly lower than that of the control. To dentin, the microTBS of Adper Easy Bond was significantly lower than that of Adper ScotchBond SE and the control. TEM showed a tight interface to enamel for all three self-etch adhesives. A relatively thick, completely demineralized and acid-resistant hybrid layer was formed at dentin by Adper ScotchBond SE, whereas the interaction of Adper Easy Bond was much shallower, and comparable to that of so-called 'ultra-mild' self-etch adhesives. Some degree of spot- and cluster-like nano-leakage was observed for both adhesives, but did not differ in extent and form from that observed for the control. CONCLUSIONS: Although the new two self-etch adhesives revealed a tight interaction at both enamel and dentin, their bond strength to both tooth tissues was generally lower than that of the control adhesive. Nevertheless, their bonding effectiveness appears in line with other simplified self-etch adhesives., English
• Polyphosphoric acid treatment promotes bone regeneration around titanium implants
  K. MAEKAWA; K. SHIMONO; M. OSHIMA; Y. YOSHIDA; B. VAN MEERBEEK; K. SUZUKI; T. KUBOKI, Journal of Oral Rehabilitation, 36, 5, 362, 367, 2009
  Wiley
• ポリリン酸を用いたチタンインプラントの生物学的表面改質
  前川 賢治; 下野 賢吾; 大島 正充; 峯 篤史; 吉田 靖弘; 鈴木 一臣; 窪木 拓男, 日本口腔インプラント学会誌, 21, 特別号, 242, 242, Aug. 2008
  (公社)日本口腔インプラント学会, Japanese
• Bactericide delivery system for preventing dental caries and periodontal disease
  NAMBA Naoko; YOSHIDA Yasuhiro; MATSUURA Kaori; ITO Takashi; MAEDA Hiroshi; ARAI Hideo; SUZUKI Kazuomi; TAKASHIBA Shogo, 日本歯科保存学雑誌, 51, 春季特別, 44, 44, 07 May 2008
  (NPO)日本歯科保存学会, Japanese
• P-6 Antibacterial effect of CPC immobilized in the resin matrix
  NAMBA N; YOSHIDA Y; NAGAOKA N; SUZUKI K; TAKASHIBA S, Journal of the Japanese Society for Dental Materials and Devices, 27, 2, 141, 141, 31 Mar. 2008
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Antibacterial effect of CPC immobilized in the resin matrix
  Namba Naoko; Yoshida Yasuhiro; Nagaoka Noriyuki; Suzuki Kazuomi; Takashiba Shogo, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 2008s, 37, 37, 2008
  レジンに添加した殺菌剤は，徐放されることにより抗菌効果を発揮する。しかしながら，固定された殺菌剤の抗菌性については定かではない。本研究では，殺菌剤CPCを固定したときの効果についてS.mutansを用いて検討した。その結果，CPC添加レジンは，硬化後，CPC溶出量が検出限界以下であったにも関わらず，十分な抗菌効果を発揮していた。これより，表面に固定された殺菌剤も抗菌効果を発揮することが示唆された。, The Japanese Society for Dental Materials and Devices, Japanese
• 陽極酸化Ti-6Al-4V合金の表面解析
  山上哲賢; 鈴木一臣; 吉田靖弘; 長岡紀幸; 脇山智子, 日本歯科医師会雑誌, 61, 5, 540, 540, 2008
  (公社)日本歯科医師会, Japanese
• The Quasi-3D Marginal Leakage of Full-coverage Crowns: Resin Coating Method Vs Sodium Hypochlorite Treatment.
  Kawahara D; Mine A; De Munck J; Kuboki T; Yoshida Y; Suzuki K; Van Meerbeek B; Yatani H, Journal of Dentistry, 2008
• 歯質接着界面のナノスケールにおける解析
  吉田靖弘; 鈴木一臣, The Journal of Dental Engineering, 165, 26, 28, 2008
• Guideline for Selection of Dentin Adhesives and Luting Agents
  MIYAZAKI Takashi; MATSUMURA Hideo; MOMOI Yasuko; KADOMA Yoshinori; NISHIYAMA Norihiro; YOSHIDA Yasuhiro; SUZUKI Kazuomi, 日本歯科医学会誌, 27, 27, 35, 39, 2008
  日本歯科医学会, Japanese
• 【接着性コンポジットレジン修復の基礎と臨床】基礎編 接着の進歩と研究・開発の現状 象牙質への接着 化学的接着の見地から
  吉田 靖弘; 鈴木 一臣; 宮崎 真至, 日本歯科評論, 特別号, 85, 92, Nov. 2007
  (株)ヒョーロン・パブリッシャーズ, Japanese
• チタン界面反応のナノレベル解析のためのチタンバイオセンサーの開発
  平田 伊佐雄; 日浅 恭; 阿部 靖彦; 赤川 安正; 岡崎 正之; 吉田 靖弘; 窪木 拓男; 鈴木 一臣, 日本バイオマテリアル学会大会予稿集, 29回, 365, 365, Nov. 2007
  日本バイオマテリアル学会, Japanese
• Titanium surface modification by phosphorylated saccharide for bioactive oral implant
  TANAKA T.; YOSHIDA Y.; TSUCHIMOTO Y.; SUZUKI K.; KUBOKI T., Journal of the Japanese Society for Dental Materials and Devices, 26, 2, 172, 172, 25 Apr. 2007
  本実験では、チタンインプラントの新規表面改質材料として、リン酸化プルランを用い検討した。細胞実験では、リン酸化プルラン処理チタンプレート上でマウス骨芽細胞様細胞の増殖特性を検討したところコントロールと比べ有意に高い増殖反応を認めた。さらに、細胞内シグナル伝達経路のひとつであるMAPKにリン酸化プルランがおよぼす影響を検討したところ、MAPKのなかでもERKの活性がリン酸化プルランチタンプレート上で亢進していた。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Chemical, mechanical, and biological properties of titanium implant surface treated with polyphosphoric acid.
  Maekawa Kenji; Yoshida Yasuhiro; Hirata Isao; Okazaki Masayuki; Suzuki Kazuomi; Kuboki Takuo, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 2007f, 205, 205, 2007
  We evaluated the chemical, mechanical, and biological properties of polyphosphoric acid (PPA) adsorbed titanium (Ti) implants. The research data suggeted the possibilities that the PPA pre-treatment of Ti surface could be useful for fabricating the bioactive Ti implant., The Japanese Society for Dental Materials and Devices, English
• Modification of Ti surface with phosphorylated pullulan and photoreactive gelatin
  Yoshida Yasuhiro; Tanaka Tsutomu; Kuboki Takuo; Suzuki Kazuomi; Ito Yoshihiro, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 2007f, 38, 38, 2007
  The aim of this study was to evaluate the effect of treating Ti with phosphorylated pullulan or photo-reactive gelatin on the cell attachment and proliferation. Adsorption of phosphorylated pullulan significantly accelerated cell proliferation. It was found that the growth of cells on titanium could be regulated by pattern-immobilized gelatin. These facts indicate that these materials can be used to fabricate bioactive Ti implants., The Japanese Society for Dental Materials and Devices, English
• Cell proliferation on Titania layer with in vitro apatite forming ability
  S. Yabe; K. Tsuru; S. Hayakawa; A. Osaka; Y. Yoshida; K. Suzuki; T. Kuboki, BIOCERAMICS, VOL 19, PTS 1 AND 2, 330-332, 131, +, 2007
  English
• 【臨床に役立つ 接着修復のすべて】臨床に役立つ接着の基礎理論 歯質への化学的接着を科学する
  吉田 靖弘; 鈴木 一臣, 歯界展望, 別冊, 臨床に役立つ接着修復のすべて, 45, 50, Oct. 2006
  医歯薬出版(株), Japanese
• Long-term Bonding Effectiveness of 1-step Adhesives to Dentin
  FUKUOKA Anri; KOSHIRO Kenichi; INOUE Satoshi; YOSHIDA Yasuhiro; TANAKA Toru; IKEDA Takatsumi; SUZUKI Kazuomi; VAN MEERBEEK Bart; SANO Hidehiko, 日本歯科保存学雑誌, 49, 秋季特別, 83, 83, Oct. 2006
  (NPO)日本歯科保存学会, Japanese
• Biological modification of the Ti surface with photoreactive gelatin
  YOSHIDA Y.; SUZUKI K., Journal of the Japanese Society for Dental Materials and Devices, 25, 5, 321, 321, 25 Sep. 2006
  Ti was biologically modified with photoreactive gelatin in order to regulate the attachment of cells. Photoreactive gelatin, which was synthesized by the coupling reaction of gelatin with N-(4-azidobenzoyloxy) succiminide, was immobilized onto Ti surface by ultraviolet (UV) irradiation. With the photo mask in place, micropatterning of the gelatin was performed. This pattern was confirmed by optical microscopy and time-of-flight secondary ion-mass spectroscopy (TOF-SIMS). Monkey COS-7 epithelial cells and mouse STO fibroblast cells were cultured on the pattern-immobilized plate. A significantly higher degree of cell attachment was found on the photoreactive gelatin-immobilized regions than on those that were not immobilized., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• チタン表面の生物学的改質による細胞接着制御
  吉田 靖弘; 伊藤 嘉浩; 蓮田 寛和; Heydari Mojgan; 鈴木 一臣, 日本口腔インプラント学会誌, 19, 3, 323, 324, Sep. 2006
  (公社)日本口腔インプラント学会, Japanese
• チタン表面と生体分子の相互作用解析のためのバイオセンサーの開発
  平田 伊佐雄; 岡崎 正之; 吉田 靖弘; 鈴木 一臣; 前川 賢治; 窪木 拓男, 日本口腔インプラント学会誌, 19, 3, 325, 325, Sep. 2006
  (公社)日本口腔インプラント学会, Japanese
• Nano-scale Intereraction Analysis for Surface Modification of Titanium Implant with Phospho-RGD Peptide
  HIASA K; ABE Y; HIRATA I; YOSHIDA Y; SUZUKI K; OKAZAKI M; AKAGAWA Y, 日本補綴歯科学会雑誌. 特別号, 日本補綴歯科学会学術大会抄録集 = Proceedings of the ... conference, the Japan Prosthodontic Society, 50, 115, 92, 92, 08 Jul. 2006
  Japanese
• Acceleration of Cell Behavior and Growth Factor Adsorption to Titanium Implant Surface by Polyphosphoric Acid Treatment
  MAEKAWA K; YOSHIDA Y; MINE A; OSHIMA A; SHIMONO K; AKIYAMA K; FUJISAWA T; SUZUKI K; KUBOKI T, 日本補綴歯科学会雑誌. 特別号, 日本補綴歯科学会学術大会抄録集 = Proceedings of the ... conference, the Japan Prosthodontic Society, 50, 115, 91, 91, 08 Jul. 2006
  (公社)日本補綴歯科学会, Japanese
• Effect of a phosphoric acid ester monomer on resin bonding to titanium
  YOSHIDA Y.; TSUCHIMOTO Y.; FUKEGAWA D.; MINE A.; KUBOKI T.; SUZUKI K., Journal of the Japanese Society for Dental Materials and Devices, 25, 2, 143, 143, 05 Apr. 2006
  The purpose of this study was to investigate the effect of a phosphoric acid ester monomer on resin bonding to titanium. XPS demonstrated P 2p peak representing phosphate groups from MDP appeared and shifted to a lower binding energy. These facts indicate that MDP strongly and chemically adsorbed on the Ti surface. For tensile bond strength measurement, Ti plates were treated with experimental primers consisting of, respectively, MDP in concentrations of 0.1, 1 and 10wt%, or were kept untreated (control). The highest tensile bond strength of Panavia Fluoro Cement to Ti was obtained when the Ti surface was pre-treated with 10wt% MDP and was significantly higher than that when a lower concentrated MDP-based primer and control. Altogether, the data obtained strongly suggest that MDP is effective to improve the adhesive performance of resin to titanium., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Nano-scale Interaction Analysis at Titanium-Phospho-amino Acid Derivative Interfaces
  HIRATA I.; HIASA K.; ABE Y.; YOSHIDA Y.; SUZUKI K.; AKAGAWA Y.; OKAZAKI M., Journal of the Japanese Society for Dental Materials and Devices, 25, 2, 179, 179, 05 Apr. 2006
  チタンの細胞接着性を向上させるために接着因子をチタン表面に化学結合させるには、これらの結合・解離特性の解明が重要である。本研究は、リン酸化された接着因子がチタン表面に結合するメカニズムの一端を明らかにすることを目的として、リン酸化アミノ酸およびリン酸化ペプチドのチタン表面への結合・解離特性について、X線光電子分光法および表面プラズモン共鳴法を用いて表面科学的検討を行った結果について報告する。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• う蝕・歯周病予防のための抗菌性コーティング材の開発
  吉田 靖弘; 鈴木 一臣, 日本歯科医学会誌, 25, 130, 130, Mar. 2006
  日本歯科医学会, Japanese
• 骨芽細胞様細胞株(MC3T3E1細胞)の細胞接着,増殖,分化および遺伝子発現に対するチタンの影響
  秋山 謙太郎; 藤澤 拓生; 明貝 文夫; 完山 学; 吉田 靖弘; 高柴 正悟; 鈴木 一臣; 窪木 拓男, 日本口腔インプラント学会誌, 19, 1, 32, 32, Mar. 2006
  (公社)日本口腔インプラント学会, Japanese
• 表面プラズモン共鳴のためのリン酸カルシウムおよび各種金属センサーの開発
  吉田靖弘; 鈴木一臣; 尾坂明義; 早川聡; 石川邦夫; 岡崎正之; 平田伊佐雄, 中谷電子計測技術振興財団年報, 20, 2006
• 歯質接着はどこまで解明されたか？―樹脂含浸層と化学接着―
  吉田靖弘; 鈴木一臣, 日本歯科医師会雑誌, 59,605-613, 9, 835, 843, 2006
  日本歯科医師会, Japanese
• 歯質接着材のナノスケール構造解析
  吉田靖弘, バイオマテリアル－生体材料－, 24,257-265, 4, 257, 265, 2006
  資料形態 : テキストデータ プレーンテキスト
  コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 雑誌, 日本バイオマテリアル学会, Japanese
• チタンの接着耐久性を向上する新しい被着面処理法
  土本 洋平; 吉田 靖弘; 峯 篤史; 矢谷 博文; 鈴木 一臣; 窪木 拓男, 日本補綴歯科学会雑誌, 49, 114回特別, 142, 142, Oct. 2005
  (公社)日本補綴歯科学会, Japanese
• Real-time analysis between titanium and bio-interactive molecules using surface plasmon resonance
  HIRATA I.; NOMURA Y.; WAKASA K.; OKAZAKI M.; YOSHIDA Y.; SUZUKI K.; MAEKAWA K.; KUBOKI T., Journal of the Japanese Society for Dental Materials and Devices, 24, 5, 358, 358, 05 Sep. 2005
  表面プラズモン共鳴解析法は、表面近傍における物質のナノレベルでの吸着・脱離過程をリアルタイムで直接観察可能な優れた方法である。前発表では、表面プラズモン共鳴解析装置のためのチタンバイオセンサーの開発について報告した。今回は、開発に成功したチタンバイオセンサーを用いて、チタン表面と組織接着性因子とのリアルタイムな相互作用の解析を行った結果について報告する。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• ヒドロキシアパタイトとリン酸エステル系機能性モノマーの化学的相互作用
  泓川 大輔; 吉田 靖弘; 鈴木 一臣; 早川 聡; 尾坂 明義; Van Meerbeek Bart, 歯科材料・器械, 24, 5, 338, 338, Sep. 2005
  (一社)日本歯科理工学会, Japanese
• トータルエッチングシステムによる樹脂含浸象牙質層の違いが長期接着耐久性に及ぼす影響
  吉田 靖弘; 鈴木 一臣, 歯界展望, 特別号, 健康な心と身体は口腔から-発ヨコハマ2004-, 318, 318, Jun. 2005
  医歯薬出版(株), Japanese
• トータルエッチングシステムのステップ数と樹脂含浸層の違いが象牙質への接着耐久性に及ぼす影響
  吉田 靖弘; 岡崎 まさみ; 中村 真理子; 畑中 乾志; 橋本 俊明; 中條 新次郎; 入江 正郎; 田仲 持郎; 鈴木 一臣, 岡山歯学会雑誌, 24, 1, 12, 13, Jun. 2005
  岡山歯学会, Japanese
• 水酸化カルシウムあるいはレジン系接着システムによる直接覆髄が臓器に及ぼす影響
  吉田 靖弘; 鈴木 一臣, 日本歯科保存学雑誌, 48, 春季特別, 67, 67, May 2005
  (NPO)日本歯科保存学会, Japanese
• Acceleration of cell behavior and growth factor adsorption onto titanium implant surface by polyphosphoric acid treatment
  MAEKAWA K.; YOSHIDA Y.; HIRATA I.; OKAZAKI M.; SUZUKI K.; KUBOKI T., Journal of the Japanese Society for Dental Materials and Devices, 24, 2, 90, 90, 05 Apr. 2005
  種々の細胞の増殖や，骨芽細胞の石灰化を亢進することが報告されているポリリン酸塩の生理活性に着目し，ポリリン酸のチタン表面吸着を試みたところ，この高分子は強固にチタン表面に科学吸着することをX線光電子分光分析法で確認するとともに，骨髄由来幹細胞の細胞接着を亢進することを見出した．さらに，このポリリン酸を担体として細胞成長因子であるbFGFをチタン表面に吸着させることに成功した．, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Effect of different functional monomers on hydrolytic stability of self-etching priming systems bonded to dentin
  YOSHIDA Y.; INOUE S.; KOSHIRO K.; SANO H.; SUZUKI K.; VAN MEERBEEK B., Journal of the Japanese Society for Dental Materials and Devices, 24, 2, 149, 149, 05 Apr. 2005
  The aim of this study was to characterize the adhesive performance of three functional monomers, 10-MDP, 4-MET, and phenyl-P, with hydroxyapatite, and to determine the hydrolytic stability of 3 self-etch adhesives, Clearfil MegaBond (CMB), Unifil Bond (UFB) or Clearfil Liner Bond II (LBII). Among these monomers, 10-MDP appeared not only to interact most intensively with hydroxyapatite; its bond with calcium appeared also most hydrolytically stable. Even after 100000 cycles, the 10-MDP-based adhesive (CMB) revealed an micro-TBS of 35.3 MPa, which was not significantly different from the control micro-TBS (40.8 MPa). The 4-MET-based adhesive (UFB) showed statistically the same micro-TBS as the control (37.9 MPa) up to 50000 cycles, but after 100000 cycles its micro-TBS was significantly lower (22.5 MPa). For the phenyl-P-based adhesive (LBII), the micro-TBS decreased significantly after 30000 cycles from the control value (44.7 MPa) towards 23.2 MPa after 100000 cycles., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Technique-sensitivity of contemporary adhesives
  B van Meerbeek; K van Landuyt; J de Munck; M Hashimoto; M Peumans; P Lambrechts; Y Yoshida; S Inoue; K Suzuki, DENTAL MATERIALS JOURNAL, 24, 1, 1, 13, Mar. 2005
  English, Book review
• より豊かな生活に貢献する医療技術に関する研究 骨形成促進因子の表面修飾を応用したチタンインプラントの機能制御と組織再生能評価
  吉田 靖弘; 鈴木 一臣, 医科学応用研究財団研究報告, 22, 104, 108, Feb. 2005
  チタンインプラントの更なる機能向上と治癒期間短縮を目指して,骨誘導能を有する生理活性物質をチタン表面に化学吸着させるバイオミメティック表面改質法を新たに確立するために,チタン表面に種々の処理を行い表面分析ならびに細胞実験にてその機能を評価した.ポリリン酸は水溶液の酸性度が強いため,チタン酸化膜表面の水酸基は正に帯電し,強固に吸着したものと考えられた.また,ポリリン酸にて表面修飾されたチタンは負に帯電することから,等電点が約9.6であるbFGFをチタンインプラント表面に吸着させ,徐放させることにも有用であると考えられた, (公財)鈴木謙三記念医科学応用研究財団, Japanese
• チタンの接着耐久性を向上する新しい被着面処理法
  土本洋平; 吉田靖弘; 峯篤史; 矢谷博文; 鈴木一臣; 窪木拓男, 日本補綴歯科学会学術大会プログラム・抄録集, 114th, 2005
• Effects of inorganic ion adsorption on the in vitro bioactivity of nano-crystalline titanium oxide layer
  Matsuura Naoko; Tsuru Kanji; Hayakawa Satoshi; Osaka Akiyoshi; Yoshida Yasuhiro; Suzuki Kazuomi; Kuboki Takuo, Preprints of Annual Meeting of The Ceramic Society of Japan
  Preprints of Fall Meeting of The Ceramic Society of Japan, 18th, 828, 828, 2005
  金属チタン系インプラント材料は骨と直接結合しないが，チタンを化学処理して得られるナノ結晶性酸化チタン層はサブミクロンスケールの多孔質表面構造をもち，優れたin vitroアパタイト形成能（生体活性）を示す。そこでチタンインプラントにこの層を生成させ，さらにアパタイトの成分であるCa(II)とP(V)とを吸着させれば，生体活性が一層向上し，細胞接着性が増進され，新しい機能性インプラント材料の開発に繋がると考えた。本研究ではイオン吸着がアパタイト核形成誘導期間に及ぼす影響について調べ，細胞の挙動に影響を与えると考えられるイオン吸着条件を検討した。その結果，リン酸イオンを吸着させた試片のアパタイト形成能は低下することが分かった。, The Ceramic Society of Japan
• Chemical interaction between hydroxyapatite and functional phosphoric acid ester monomers
  FUKEGAWA D.; YOSHIDA Y.; SUZUKI K.; HAYAKAWA S.; OSAKA A.; VAN MEERBEEK B., Journal of the Japanese Society for Dental Materials and Devices, 24, P, 35, 2005
  We examined the chemical interaction of 10-MDP and a hydroxyapatite (HAp) particle used for a self-etching system by various structural analysis techniques. In early period of reaction, 10-MDP chemisorbed on the calcium sites of the HAp surface. The 10-MDP monomers reacted with calcium ions dissolved from HAp due to the decalcification function of a 10-MDP monomer and resulted in the deposition of calcium salt (MDPCa) of 10-MDP on HAp. As reaction advances, calcium hydrogenphosphate dihydrate (DCPD) also deposited on the MDPCa. The dissolution (decalcification) of HAp by a 10-MDP monomer and the deposition of calcium salts were caused on the HAp surface., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Nano-technological Control of Biomaterial-Hard Tissue Interfaces
  Yasuhiro Yoshida; Kazuomi Suzuki; Bart Van Meerbeek; Yoshihiro Ito; Yoshihiro Ito, Journal of Hard Tissue Biology, 14, 2, 265, 266, 2005
  English
• 硬組織疾患に対する新規治療薬開発のための有機材料・生体硬組織無機成分界面のナノレベルにおける構造解析
  吉田 靖弘, 上原記念生命科学財団研究報告集, 18, 404, 406, Nov. 2004
  骨補填材としてのアパタイトの更なる機能向上と治癒期間短縮を目指し,表面性状や吸着分子をナノレベルで制御することにより,成長因子を局在させるためのドラッグデリバリーシステムとしての役割と骨形成時のCaやリンの供給源としての両方の機能を兼ね備えた骨誘導型機能性アパタイト系インプラントを開発した.Mg2+が生体接着分子の結合に関与していることに着目し,独自に開発した合成装置を用いて傾斜機能性Mg含有炭酸アパタイトを合成した.細胞接着実験の結果,このアパタイト粉体は骨の無機成分である炭酸アパタイトよりも骨芽細胞の接着性に優れていた.動物実験の結果,Mg含有炭酸アパタイトでは骨密度が高く,かつ,十分な厚みのある骨再生が成された.これよりアパタイト表層の化学状態は生体接着分子の吸着に影響を及ぼすこと,Mgは接着分子の吸着に有用で,また本材料は骨補填材として有用であることが示唆された, (公財)上原記念生命科学財団, Japanese
• インプラント表面における生体調節因子の分子配列制御とES細胞の分化誘導—平成14年度助成 研究成果特集 ; 学術研究(第40回)
  吉田 靖弘, 公益財団法人山陽放送学術文化財団リポート / 山陽放送学術文化財団 編, 48, 34, 38, Oct. 2004
  岡山 : 山陽放送学術文化財団, Japanese
• Effect of Er:YAG laser irradiation on bonding, structural and mechanical properties of the tooth enamel
  MINE A.; SUZUKI K.; YATANI H.; YOSHIDA Y.; KUBOKI T., Journal of the Japanese Society for Dental Materials and Devices, 23, 5, 361, 361, 10 Sep. 2004
  Er:YAGレーザーを照射したエナメル質の性質がどのように変化し，また被着体とした場合の接着について十分に解明されていない．そこでレーザー照射エナメル質をSEM，EDX，XPS，XRD，万能試験機を用い物理的および化学的に把握し，接着に及ぼす影響について検討した．その結果，レーザー照射によりエナメル質は機械的に脆弱になり，特に低い照射出力の場合はこの事象が接着に影響することが明らかとなった．, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Detrimental effects of direct pulp capping agents on organic tissues
  NAKAMURA M.; OKAZAKI M.; YOSHIDA Y.; SUZUKI K.; ISHIKAWA K., Journal of the Japanese Society for Dental Materials and Devices, 23, 5, 341, 341, Sep. 2004
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• 象牙質接着の長期安定性 トータルエッチングシステムにおける水中浸漬後の接着強さ
  吉田 靖弘; 鈴木 一臣; De Munck Jan; Van Meerbeek Bart, 日本歯科医師会雑誌, 57, 4, 423, 423, Jul. 2004
  (公社)日本歯科医師会, Japanese
• Four-year water degradation of a resin-modified glass-ionomer adhesive bonded to dentin (vol 112, pg 73, 2004)
  J De Munck; B Van Meerbeek; Y Yoshida; S Inoue; K Suzuki; P Lambrechts, EUROPEAN JOURNAL OF ORAL SCIENCES, 112, 2, 205, 205, Apr. 2004
  English, Others
• Influence of hybridization effectiveness on long-term durability of total-etch adhesives bonded to dentin
  YOSHIDA Y.; SUZUKI K., Journal of the Japanese Society for Dental Materials and Devices, 23, 2, 132, 132, 20 Mar. 2004
  象牙質へのレジン浸透効果を解明するため，2-および3-ステップトータルエッチングシステムにより形成された樹脂含浸象牙質層の違いが4年間の水中浸漬後の象牙質接着強さに及ぼす影響について検討した。その結果，エナメル質とレジンとの接着により辺縁封鎖場合には両者の接着強さには優位さは認められなかったが，辺縁封鎖のない場合では3-ステップに比べ2-ステップのシステムでは接着強さの優位な低下が認められた。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Detrimental effects of direct pulp capping agents on organic tissues
  Nakamura Mariko; Yoshida Yasuhiro; Okazaki Masami; Suzuki Kazuomi; Ishikawa Kunio, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 2004f, 21, 21, 2004
  直接覆髄剤は血液と直接接するため生体への影響が大きいと考えられるので、為害作用が少ない薬剤であることが望まれる。本研究はラット第一臼歯を露髄させ水酸化カルシウム製剤などにより直接覆髄した後、一定期間経過後心臓より採血を行い、肝臓などを摘出し、血液検査ならびに臓器の病理学的検査を行うことにより直接覆髄剤が生体に及ぼす影響の調査を行うことを目的とした。, The Japanese Society for Dental Materials and Devices
• Adhesion to enamel and dentin. Current state and future tasks - Part 2
  B. Van Meerbeek; J. De Munck; Y. Yoshida; S. Inoue; M. Vargas; P. Vijay; K. Van Landuyt; P. Lambrechts; G. Vanherle, Asthetische Zahnmedizin, 7, 2, 95, 109, 2004
  German, Book review
• Adhesion to enamel and dentin. Current state and future tasks - Part 1
  B. Van Meerbeek; J. De Munck; Y. Yoshida; S. Inoue; M. Vargas; P. Vijay; K. Van Landuyt; P. Lambrechts; G. Vanherle, Asthetische Zahnmedizin, 7, 1, 4, 18, 2004
  German, Book review
• マウスES細胞を用いたin vitro歯はい組織誘導システムの検討
  土本洋平; 田川陽一; 完山学; 神吉昭子; 吉田靖弘; 鈴木一臣; 窪木拓男, 日本分子生物学会年会プログラム・講演要旨集, 27th, 2004
• 次世代の歯質接着材料・技術の展望と課題.
  鈴木一臣; 吉田靖弘, 日本歯科医師会雑誌, 57,3,217-225, 3, 217, 225, 2004
  日本歯科医師会, Japanese
• 歯質接着機構のナノスケールにおける解明.
  吉田靖弘; Bart Van Meerbee; 鈴木一臣, ザ・クインテッセンス, 23,4,57-63, 4, 57, 63, 2004
  クインテッセンス出版(株), Japanese
• 歯根破折防止に繋がる?ファイバーポストの臨床
  山下 敦; シルビア・ヨシガ; 峯 篤史; 土本 洋平; 函城 貴仁; 園山 亘; 大山 龍男; 矢谷 博文; 吉田 靖弘; 鈴木 一臣; 窪木 拓男, 日本補綴歯科学会雑誌, 47, 5, 901, 901, Dec. 2003
  (公社)日本補綴歯科学会, Japanese
• Micro-tensile bond strength of glass-ionomer adhesive to dentin after 4-year water storage.
  B Van Meerbeek; J De Munck; Y Yoshida; S Inoue; P Lambrechts, JOURNAL OF DENTAL RESEARCH, 82, 501, 501, Dec. 2003
  English, Summary international conference
• A Mechanically Weak Layer (Laser Smear Layer) of the Laser-irradiated Dentin and Bond Strengths
  MINE A; SUZUKI K; YATANI H; YOSHIDA Y; KUBOKI T, 日本補綴歯科學會雜誌 = The journal of the Japan Prosthodontic Society, 47, 110回特別, 69, 69, 24 Oct. 2003
  (公社)日本補綴歯科学会, Japanese
• Development of a new adhesive system to titanium surface
  TSUCHIMOTO Y.; YOSHIDA Y.; MINE A.; KUBOKI T.; YATANI H.; SUZUKI K., Journal of the Japanese Society for Dental Materials and Devices, 22, 5, 343, 343, 25 Aug. 2003
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Development of self-setting calcium hydroxide preparation (PART 3) - Effect of self-setting calcium hydroxidepreparation on dental pulp
  NAKAMURA M.; ISHIKAWA K.; YOSHIDA Y.; SUZUKI K., Journal of the Japanese Society for Dental Materials and Devices, 22, 5, 408, 408, Aug. 2003
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Osteoblast invasion into CO(3)Ap-collagen sponges.
  H. Shintani; M. Itoh; I. Hirata; Y. Yoshida; A. Shimazu; M. Okazaki, JOURNAL OF DENTAL RESEARCH, 82, B139, B139, Jun. 2003
  English, Summary international conference
• Comparative study on adhesive performance of functional monomers.
  Y. Yoshida; B. Van Meerbeek; M. Okazaki; H. Shintani; K. Suzuki, JOURNAL OF DENTAL RESEARCH, 82, B19, B19, Jun. 2003
  English, Summary international conference
• Chemical bonding potential of adhesive materials to hydroxyapatite.
  B. Van Meerbeek; J. De Munck; Y. Yoshida; K. Shirai; S. Inoue; H. Shintani; P. Lambrechts, JOURNAL OF DENTAL RESEARCH, 82, B101, B101, Jun. 2003
  English, Summary international conference
• Effect of surface Treatment on Adhesive Properties Titanium Dental Prosthesis
  TSUCHIMOTO Y; YOSHIDA Y; MINE A; KUBOKI T; YATANI H; SUZUKI K, 日本補綴歯科學會雜誌 = The journal of the Japan Prosthodontic Society, 47, 109回特別, 85, 85, 09 May 2003
  (公社)日本補綴歯科学会, Japanese
• Long-term Durability of Resin-dentin Bond in Class I Cavity Using Various Adhesive Systems
  SHIRAI Kenichi; MOTOYAMA Tomotoku; SHINTANI Takahide; ITOH Manabu; YOSHIDA Yasuhiro; INOUE Satoshi; Meerbeek Bart Van; FUJITANI Morioki; SHINTANI Hideaki, 日本歯科保存学雑誌, 46, 春季特別, 121, 121, 06 May 2003
  (NPO)日本歯科保存学会, Japanese
• Buonocore Memorial Lecture - Adhesion to enamel and dentin: Current status and future challenges
  B Van Meerbeek; J De Munck; Y Yoshida; S Inoue; M Vargas; P Vijay; K Van Landuyt; P Lambrechts; G Vanherle, OPERATIVE DENTISTRY, 28, 3, 215, 235, May 2003
  English, Book review
• Bonding and technology to hard tissue for next generation
  SUZUKI K.; OHNO H.; KADOMA Y.; KURATA S.; NISHIYAMA N.; HIRABAYASHI S.; YOSHIDA Y., Journal of the Japanese Society for Dental Materials and Devices, 22, 2, 119, 119, 20 Mar. 2003
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Cells invasion into carbonated apatite/collagen composite sponges
  ITOH M.; SHIMAZU A.; HIRATA I.; YOSHIDA Y.; OKAZAKI M.; SHINTANI H., Journal of the Japanese Society for Dental Materials and Devices, 22, 2, 105, 105, 20 Mar. 2003
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Shrinkage kinetics of acid etched dentin (part2) Measurement of the heigh of dentin disk using a contact probe and a non-contact probe
  ITOU K.; TORII Y.; YOSHIDA Y.; SUZUKI K.; YOSHIDA M.; PASHLEY David H., Journal of the Japanese Society for Dental Materials and Devices, 22, 2, 65, 65, 20 Mar. 2003
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Effect of surface treatment on adhesion to titanium prosthesis
  Tsuchimoto Yohei; Yoshida Yasuhiro; Mine Atsushi; Kuboki Takuo; Yatani Hirohumi; Suzuki Kazuomi, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 2003f, 56, 56, 2003
  近年、チタンは歯科用金属として使用頻度が高くなっているが、接着特性に関して断片的にしか検討されていない。本研究では、チタンに対する表面処理法の違いが接着性能に及ぼす影響について検討した。表面化学分析の結果、塩酸処理後のチタン表面には、より多くのMDPが化学吸着していた。引張り試験の結果は、塩酸処理群は熱負荷後において有意に高い値を示し、表面化学分析の結果を裏付けるものとなった。, The Japanese Society for Dental Materials and Devices
• Osteoblast invasion into carbonated apatite-collagen sponges
  Itoh Manabu; Shimazu Atushi; Hirata Isao; Yoshida Yasuhiro; Okazaki Masayuki; Shintani Hideaki, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 2003s, 325, 325, 2003
  炭酸アパタイトとゲル化したコラーゲンを混合し，凍結乾燥を行うことで，高気孔率を有するスポンジ状の複合体が創製した。その複合体スポンジに骨芽細胞を播種したところ，細胞は複合体のかなり深部にまで中に侵入していることが確認された。, The Japanese Society for Dental Materials and Devices
• Shrinkage kinetics of acid etched dentin (part2)
  ITOU KOUSUKE; TORII YASUHIRO; YOSHIDA YASUHIRO; SUZUKI KAZUOMI; YOSHIYAMA MASAHIRO; PASHLEY DAVID, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 2003s, 115, 115, 2003
  今回の実験では接触および非接触型プローブを用いて酸処理象牙質の収縮動態の測定を行った．その結果，接触および非接触型プローブでは結果が異なり，酸処理象牙質の変化を測定するためには非接触型プローブでの測定が好ましいと考えられた．, The Japanese Society for Dental Materials and Devices
• Development of self-setting calcium hydroxide preparation (part 3) effect of self-setting calcium hydroxidepreparation on dental pulp
  Nakamura Mariko; Ishikawa Kunio; Yoshida Yasuhiro; Suzuki Kazuomi, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 2003f, 44, 44, 2003
  我々は、水酸化カルシウムにリン酸水素カルシウムを添加し、かつ、リン酸二水素ナトリウム水溶液で練和することにより水酸化カルシウム製剤が自己硬化性を示すことを報告した。今回は、硬化しない水酸化カルシウム粉末を対照として、自己硬化型水酸化カルシウム製剤の直接覆髄剤としての効果をラットの歯を用いて病理組織学的に検討を加えた。, The Japanese Society for Dental Materials and Devices
• チタン製補綴物の接着特性に及ぼす表面処理の影響
  土本洋平; 吉田靖弘; 峯篤史; 窪木拓男; 矢谷博文; 鈴木一臣, 日本補綴歯科学会学術大会プログラム・抄録集, 109th, 2003
• Er:YAGレーザー照射象牙質の脆弱層(レーザースメア層)と接着強さ
  峯篤史; 鈴木一臣; 矢谷博文; 吉田靖弘; 窪木拓男, 日本補綴歯科学会学術大会プログラム・抄録集, 110th, 2003
• Biological examination of apatite-collagen sponges for hard tissue regeneration
  ITOH M.; SHIMAZU A.; HIRATA I.; YOSHIDA Y.; SHINTANI H.; OKAZAKI M., Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 21, 75, 75, 10 Aug. 2002
  現在臨床で用いられているアパタイト系骨補填材は多孔体ではあるが細胞の遊走や増殖に有効な気孔が十分にあるとは言えない。また, アパタイト系骨補填材では成型時, 焼結することが多く, 結晶性の著しい向上から生体内での吸収が妨げられるなどの問題もある。そこで演者らは, より生体硬組織に近い組成と結晶性を有する骨補填材を開発すべく, 結合性や柔軟性を付与した複合体の改良を続けており, 高気孔率を有するアパタイト·コラーゲン複合体の作製法を検討し, 第39回本学会において報告した。本研究では, 作製されたアパタイト·コラーゲン複合体が, ハイドロキシアパタイトの配合量で複合体内部の気孔率が変化することから, 骨再建材料としての応用の可能性を追及することを目的として, 細胞学的検討を試みた。
  以上のことより, 本研究におけるアパタイト·コラーゲン複合体には, 細胞が遊走するための連続した気孔が十分に存在しており, 骨誘導に有効で, 優れた骨補填材となりうる可能性が示唆された。, The Japanese Society for Dental Materials and Devices, Japanese
• Effect of different functional monomers on dentin adhesion of self-etching priming systems
  YOSHIDA Y.; NAGAKANE K.; FUKUDA R.; INOUE S.; SHINTANI H.; OKAZAKI M.; SANO H.; SUZUKI K.; MEERBEEK B.Van, 歯科材料・器械, 21, 20, 20, 10 Aug. 2002
  Japanese
• Effect of etching time on shrinkage and expansion of dentin etched with phosphoric acid
  ITOU K.; TORII Y.; YOSHIDA Y.; SUZUKI K.; YOSHIYAMA M.; PASHLEY D.H., Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 21, 21, 21, 10 Aug. 2002
  酸処理象牙質にレジンを接着させるためには, プライマーもしくはウェットボンデイング法を用いることが必要である. いずれの場合でも酸処理により生成したコラーゲン層の水もしくは有機溶媒等による収縮および膨潤の動態を知ることが接着機構を理解する上で重要である. 今回の実験では, 脱水および加水における過程でのリン酸処理象牙質の寸法変化を測定し, その結果より, コラーゲン層の収縮および膨潤の動態にリン酸処理時間が及ぼす影響について検討した.
  これらの結果から, リン酸により脱灰した象牙質の寸法変化は, x: 酸処理時間(sec), y: 変位量(mm)とした場合において脱水時による収縮時にはy=0.056x+0.40, R²=0.95で示され, 加水による膨潤時にはy=0.019x+0.51, R²=0.95で示された. また, 象牙質の収縮/膨潤比(=回復率, y)は酸処理時間(x)の延長に伴い低下し, y=-0.96x+95.1, R²=0.87と示された., The Japanese Society for Dental Materials and Devices, Japanese
• Dentine bonding system : Crack formation and porosity effect (Adhesion theory)
  WAKASA K.; IKEDA A.; FUKUDA R.; NOMURA Y.; YOSHIDA Y.; OKAZAKI M.; SHINTANI H.; SANO H., Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 21, 100, 100, 20 Mar. 2002
  接着理論に必要な値としての接着強さは界面にかかる強さであり, その値はmicro-tensile bond試験により評価されている。Wakasaはその試験方法をI-sectionと称しており¹⁾, そのモデルはSanoモデル²⁾とYoshiyamaモデル(カリエスを含むroot dentineにSanoモデルを適用したもの)³⁾である. Wakasaモデルではレジン/象牙質界面特性を想定して, 接着強さに与える諸因子の影響を明らかにする^4-6)。すなわち, resin composite/adhesive resin layer(bonding area)界面(界面III), adhesive resin layer/hybrid layer界面(界面II), hybrid layer/dentine界面(界面I)を想定するのがWakasaモデルである。脱灰象牙質での欠陥が指摘され⁷⁾, 試験片全体の欠陥としてのporosity分布とその影響が明らかにされた⁸⁾。porosity(microcracksと定義する)とa main crackとの相互作用(interaction energy)が接着強さに影響することを報告した⁸⁾。そこで接着理論を確立するためにmicro-tensile bond試験の結果に基づいて応力·ひずみ関係を検討して^9-12), その影響を理論的に求める。
  界面を想定するWakasaモデルを用いて, 接着試験片の諸性質と諸因子が及ぼす応力とひずみの意味を明らかにした。, The Japanese Society for Dental Materials and Devices, Japanese
• Apatite-Collagen Sponges with High-Porosity
  ITOH M; YOSHIDA Y; SHINTANI H; OKAZAKI M, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 21, 77, 77, 20 Mar. 2002
  現在, 骨再建·再生用材料には, 主として自家骨が用いられているが採取骨量の制限や骨採取手術が必要であることなど多くの問題点を残している。一方, アパタイトは供給量に問題がないことに加え, 歯質や骨など生体硬組織の無機成分として知られており, 生体組織に対して優れた親和性と骨伝導性を示すことから, 現在骨再建術に広く用いられている。生体内における移植材料は内部のスカフォールドの形状が骨誘導に大きな影響を与えることは, これまで多方面から研究され知られている。しかしながら, 現在臨床で用いられているアパタイト系骨補填材は多孔体ではあるが細胞の遊走や増殖に有効な気孔が十分にあるとは言えない。また, アパタイト系骨補填材では成型時, 焼結することが多く, 結晶性の著しい向上から生体内での吸収が妨げられるなどの問題もある。我々は, より生体硬組織に近い組成と結晶性を有する骨補填材を開発すべく, 骨の無機成分に類似したアパタイトをフィラーとし, マトリックスとしてのアテロコラーゲンを混和することにより, 結合性や柔軟性を付与した複合体の改良, 研究を続けている。しかしながら, これまでのアパタイト·コラーゲン複合体は気孔率が低く連続的な気孔を得ることが困難であった。そこで, 本研究ではアパタイト·コラーゲン複合体内部への細胞の遊走性を高めるため, 高気孔率を有するアパタイト·コラーゲン複合体の作製法を検討するとともに, 得られた複合体を三次元的に解析することを試みた。
  以上のことより, 今回作製したアパタイト·コラーゲン複合体には, 細胞が遊走するための連続した気孔が十分に存在しており, 骨誘導に有効で, 優れた骨補填材となりうる可能性が示唆された。, The Japanese Society for Dental Materials and Devices, Japanese
• Bonding Mechanism and Effectiveness of a Glass-ionomer Adhesive
  INOUE S.; VAN MEERBEEK B.; YOSHIDA Y.; SANO H., 接着歯学 = Adhesive dentistry, 19, 4, 274, 274, 15 Mar. 2002
  English
• Improved Degradation Resistance of Resin Composites through Optimized Filler-Matrix Coupling
  SHIRAI K.; YOSHIDA Y.; ITOH M.; MOTOYAMA T.; SHINTANI H.; VAN MEERBEEK B., 接着歯学 = Adhesive dentistry, 19, 4, 431, 431, 15 Mar. 2002
  English
• Micro-tensile bond strength of 4 total-etch adhesives to dentin after 4-year water storage.
  B Van Meerbeek; J De Munck; Y Yoshida; S Inoue; M Vargas; P Lambrechts; G Vanherle, JOURNAL OF DENTAL RESEARCH, 81, A138, A138, Mar. 2002
  English, Summary international conference
• Effect of different functional monomers on dentin adhesion of self-etching priming systems
  Yoshida Y.; Nagakane K.; Fukuda R.; Inoue S.; Shintani H.; Okazaki M.; Sano H.; Suzuki K.; Van Meerbeek Bart, Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices, 40, 2, 2, 2002
  近年, セルフエッチングシステムは本邦のみならず諸外国においても広く臨床応用されている。従来のエッチングとプライミングを同時に行うセルフエッチングプライマー中には, 接着性能を左右する各システム特有の機能性モノマーが含まれている。しかしながら, その違いが接着性能に及ぼす影響についてはほとんど報告されていない。本研究では, 異なる機能性モノマーを含有する3種のセルフエッチングシステムの象牙質接着性について, 形態学的, 化学的および力学的に比較検討した。
  以上より, 各セルフエッチングシステムの象牙質接着性は, 含まれる機能性モノマーによって異なり, 長期安定性を左右する可能性があることが示唆された。, The Japanese Society for Dental Materials and Devices
• 樹脂含浸層による象牙質接着の最新コンセプト
  Van Meerbeek B; De Munk J; 井上 哲; 吉田靖弘, DE, 142,21-24, 142, 21, 24, 2002
  (一社)日本歯科理工学会, Japanese
• 理工21世紀研究チーム報告 次世代の硬組織接着材料と技術
  鈴木 一臣; 西山 典宏; 平林 茂; 門磨 義則; 大野 弘機; 倉田 茂昭; 吉田 靖弘, 歯科材料・器械, 20, 特別, 56, 60, Dec. 2001
  (一社)日本歯科理工学会, Japanese
• フィラー表面洗浄によるフィラー/マトリックス界面の結合強化
  本山 智得; 白井 憲一; 新谷 英章; 吉田 靖弘; 岡崎 正之, 広島大学歯学雑誌, 33, 2, 148, 148, Dec. 2001
  広島大学歯学会, Japanese
• 象牙質接着界面の形態学的及び化学的研究 各種セルフエッチングシステムについて
  吉田 靖弘; 福田 竜一; 伊東 学; 吉岡 雅之; 白井 憲一; 新谷 英章; 井上 哲; 佐野 英彦; Meerbeek B.Van, 日本歯科保存学雑誌, 44, 秋季特別, 62, 62, Oct. 2001
  (NPO)日本歯科保存学会, Japanese
• Letters to the editor
  CS Ramesha, AMERICAN LABORATORY, 33, 20, 8, 8, Oct. 2001
  English, Report scientific journal
• Dentine bonding system : Porosity and the effect
  WAKASA K.; IKEDA A.; FUKUDA R.; NOMURA Y.; YOSHIDA Y.; OKAZAKI M.; SHINTANI H.; SANO H., 歯科材料・器械, 20, 23, 23, 15 Sep. 2001
  Japanese
• Morphological and chemical interfacial characterization of resin-modified glass-ionomer cements bonded to dentin
  YOSHIDA Y.; NAGAKANE K.; NOMURA Y.; WAKASA K.; OKAZAKI M.; SHINTANI H.; VAN MEERBEEK B., 歯科材料・器械, 20, 24, 24, 15 Sep. 2001
  Japanese
• Dentine bonding system : Stress analysis of collagen fibres at micro-tensile bond test
  WAKASA K.; IKEDA A.; FUKUDA R.; NOMURA Y.; YOSHIDA Y.; OKAZAKI M.; SHINTANI H.; SANO H., 歯科材料・器械, 20, 81, 81, 15 Mar. 2001
  Japanese
• XPS analysis of filler-silane coupling agent interfaces
  YOSHIDA Y.; SHIRAI K.; NOMURA Y.; WAKASA K.; OKAZAKI M.; SHINTANI H., 歯科材料・器械, 20, 87, 87, 15 Mar. 2001
  Japanese
• Genotoxicity and estrogenicity of polymerization initiator in dental resin in vitro
  NOMURA Y.; SHIRAISHI F.; JAN C. Moin; URABE H.; YOSHIDA Y.; WAKASA K.; OKAZAKI M., 歯科材料・器械, 20, 40, 40, 15 Mar. 2001
  Japanese
• Mg含有アパタイトの合成および細胞接着特性
  山崎 保彦; 吉田 靖弘; 野村 雄二; 岡崎 正之; 久保 隆靖; 赤川 安正; 内田 隆; 里田 隆博; 島津 篤; 濱田 吉之輔, 広島大学歯学雑誌, 32, 2, 120, 120, Dec. 2000
  広島大学歯学会, Japanese
• 光重合型レジンにおける重合開始剤の溶出に関する研究
  Jan C.Moin; 占部 秀徳; 吉岡 雅之; 新谷 英章; 野村 雄二; 吉田 靖弘; 若狭 邦男; 岡崎 正之, 広島大学歯学雑誌, 32, 2, 120, 120, Dec. 2000
  広島大学歯学会, Japanese
• 歯質に対するポリカルボン酸の化学的接着能
  福田 竜一; 新谷 英章; 吉田 靖弘; 野村 雄二; 若狭 邦男; 岡崎 正之; Meerbeek B.Van, 広島大学歯学雑誌, 32, 2, 121, 121, Dec. 2000
  広島大学歯学会, Japanese
• Microscopy investigations. Techniques, results, limitations
  B Van Meerbeek; M Vargas; S Inoue; Y Yoshida; J Perdigao; P Lambrechts; G Vanherle, AMERICAN JOURNAL OF DENTISTRY, 13, 3D, 18D, Nov. 2000
  English
• Bonding Mechanisms of Glass-ionomer Cements to Tooth Structure : Chemical Bonding Potential of Copolymers
  YOSHIDA Yasuhiro; FUKUDA Ryuichi; YOSHIOKA Masayuki; SHIRAI Kenichi; SHINTANI Hideaki, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 43, 秋季特別, 16, 16, 17 Oct. 2000
  (NPO)日本歯科保存学会, Japanese
• A histological study on biocompatibility of functionally graded MgCO_3apatite-collagen composite
  YAMASAKI Y.; YOSHIDA Y.; NOMURA Y.; OKAZAKI M.; AKAGAWA Y.; SHIMAZU A.; HAMADA Y., 歯科材料・器械, 19, 53, 53, 20 Sep. 2000
  Japanese
• 残存象牙質の厚めがレジン接着用光硬化型グラスアイオノマーセメントの微小引張り接着強さに及ぼす影響(共著)
  井上 哲; Van Meerbeek Bart; 阿部 泰彦; 吉田 靖弘; Lambrechts Paul; Vanherle Guido; 佐野 英彦, 接着歯学, 18, 2, 93, 99, Aug. 2000
  残存象牙質の厚み(RDT)が,レジン接着用光硬化型グラスアイオノマーセメントの微小引張り接着強さ(MTBS)に及ぼす影響を検討した.14歯のヒト抜去歯から得た平坦象牙質面に,Fuji BOND LC(ジーシー)を用いて,コンディショナー処理の有無の条件下でコンポジットレジンを接着した.37℃で24時間水中保存後,試料をダンベル型に調整し,微小引張り試験を行った.その結果,RDTが大きくなると,有意に高いMTBSを示した.又,コンディショナー未処理群よりも処理群の方が,有意に高いMTBSを示した.更に,処理群においては,RDTに拘わらず,本材とコンポジットレンジ間の界面破壊を多く認めた.未処理群では,本材とコンポジットレンジ間での界面破壊を認めず,象牙質と本材間の界面破壊及び混合破壊を多く認めた, (一社)日本接着歯学会, Japanese
• Surface properties of functionally graded MgCO_3apatite-collagen composite and its cell adhesion behavior
  YAMASAKI Y.; YOSHIDA Y.; NOMURA Y.; OKAZAKI M.; AKAGAWA Y.; SHIMAZU A.; HAMADA Y., 歯科材料・器械, 19, 35, 152, 152, 01 Apr. 2000
  Japanese
• Assessment of decontamination methods of filler particles for dental resin composites
  ITOH M.; YOSHIDA Y.; SHIRAI K.; FUJITANI M.; OKAZAKI M.; SHINTANI H., 歯科材料・器械, 19, 35, 142, 142, 01 Apr. 2000
  Japanese
• Studies on dissolution of visible light-cured resin (Part 3) Relationship between radical generation and leachability of CQ
  NOMURA Y.; MOIN JAN C.; URABE H.; YOSHIOKA M.; YOSHIDA Y.; WAKASA K.; SHINTANI H.; OKAZAKI M., 歯科材料・器械, 19, 35, 158, 158, 01 Apr. 2000
  Japanese
• Dentine bonding system - Stress analysis of demineralized dentine at micro - tensile bond test -
  WAKASA K.; YOSHIDA Y.; IKEDA A.; SHIRAI K.; YOSHIOKA M.; MATSUI A.; YAMAKI M.; SANO H.; SANO H., 歯科材料・器械, 19, 35, 31, 31, 01 Apr. 2000
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Bonding mechanism micro-tensile bond strength of 4-MET-based self-etching adhesive.
  S Van Meerbeek; Y Yoshida; S Inoue; M Vargas; Y Abe; R Fukuda; M Okazaki; P Lambrechts; G Vanherle, JOURNAL OF DENTAL RESEARCH, 79, 249, 249, 2000
  English, Summary international conference
• Micro-tensile bond strength of an experimental resin-modified glass-ionomer adhesive.
  S Inoue; B Van Meerbeek; Y Abe; Y Yoshida; P Lambrechts; G Vanherle; H Sano, JOURNAL OF DENTAL RESEARCH, 79, 597, 597, 2000
  English, Summary international conference
• TEM evaluation of self-etching adhesives resin-dentin interfaces.
  MA Vargas; B van Meerbeek; Y Yoshida; C Bergeron; P Lambrechts; G Vanherle, JOURNAL OF DENTAL RESEARCH, 79, 268, 268, 2000
  English, Summary international conference
• Mechanism of adhesion/decalcification effects on human hard tissues by acids.
  Y Yoshida; B Van Meerbeek; M Yoshioka; P Lambrechts; G Vanherle; K Wakasa; M Okazaki, JOURNAL OF DENTAL RESEARCH, 79, 418, 418, 2000
  English, Summary international conference
• Evidence of chemical bonding at biomaterial-hard tissue interfaces
  Y. Yoshida; B. VanMeerbeek; Y. Nakayama; J. Snauwaert; L. Hellemans; P. Lambrechts; G. Vannerle; K. Wakasa, Journal of Dental Research, 79, 2, 709, 714, 2000
  Intern. and American Associations for Dental Research, English
• Effect of Remaining Dentin Thickness on Micro-tensile Bond Strength of a Resin-modified Glass-ionomer Cement for Bonding Composite Resin
  INOUE Satoshi; VAN MEERBEEK Bart; ABE Yasuhiko; YOSHIDA Yasuhiro; LAMBRECHTS Paul; VANHERLE Guido; SANO Hidehiko, Adhesive Dentistry, 18, 2, 93, 99, 2000
  The purpose of this study was to investigate the effect of remaining dentin thickness (RDT) on the micro-tensile bond strength (MTBS) to dentin of a new resin-modified glass-ionomer cement for bonding composite resin. Composite resin was bonded to flat dentin surfaces from 14 extracted human teeth using Fuji BOND LC (GC) with or without a cavity conditioner, and then sectioned to thin slabs, trimmed into dumbbell shape with the area of the interface being approximately 1mm², and subjected to micro-tensile testing at a cross-head speed of 1mm/min. MTBS to dentin significantly improved when the cavity conditioner was used and RDT increased . When the conditioner was used, failures mainly occurred adhesively at the interface between the adhesive and composite resin .When no conditioner was used, no adhesive failures between the adhesive and composite resin occurred, but failures occurred mainly adhesively between dentin and the adhesive, or mixed adhesive-cohesively. These results indicate that removal of the smear layer improves the bond strength of the glass-ionomer adhesive to dentin; Its bonding effectiveness is also affected by the area of intertubular dentin available for micro-mechanical retention through hybrid-layer formation., Japan Society for Adhesive Dentistry, Japanese
• Evidence of chemical bonding at biomaterial-hard tissue interfaces
  Y. Yoshida; B. VanMeerbeek; Y. Nakayama; J. Snauwaert; L. Hellemans; P. Lambrechts; G. Vannerle; K. Wakasa, Journal of Dental Research, 79, 2, 709, 714, 2000
  Intern. and American Associations for Dental Research, English
• 各種カルボン酸の及ぼす歯質表面Hybrid layer層への影響に関する検討 接着と脱灰メカニズムの解明
  吉岡 雅之; 新谷 英章; 吉田 靖弘; 野村 雄二; 若狭 邦男; 岡崎 正之; Meerbeek B.Van, 広島大学歯学雑誌, 31, 2, 229, 229, Dec. 1999
  広島大学歯学会, Japanese
• 象牙質接着性及び接着機序に関する基礎的研究(第13報) 微小接着強さとshrinkageの関係(micro-tensile bond testモデル)
  若狭 邦男; 吉田 靖弘; 池田 敦治; 山崎 保彦; 福田 竜一; 吉岡 雅之; 白井 憲一; 新谷 英章, 広島大学歯学雑誌, 31, 1, 188, 188, Jun. 1999
  広島大学歯学会, Japanese
• Dentine bonding system - Functional analysis of hybrid layer at micro-tensile bond test-
  WAKASA K.; YOSHIDA Y.; IKEDA A.; YAMASAKI Y.; FUKUDA R.; YOSHIOKA M.; SHIRAI K.; SANO H., 歯科材料・器械, 18, 33, 27, 27, 15 Mar. 1999
  Japanese
• Effect of tannic acid solution on collagen structures for dental restoration
  Nurhayaty Natsir; K. Wakasa; Y. Yoshida; N. Satou; H. Shintani, Journal of Materials Science: Materials in Medicine, 10, 8, 489, 492, 1999
  Kluwer Academic Publishers, English
• Porcelain veneers bonded to tooth structure: An ultra-morphological FE-SEM examination of the adhesive interface
  M. Peumans; B. Van Meerbeek; Y. Yoshida; P. Lambrechts; G. Vanherle, Dental Materials, 15, 2, 105, 119, 1999
  Elsevier Inc., English
• Hybridization effectiveness of a two-step versus a three-step smear layer removing adhesive system examined correlatively by TEM and AFM
  Bart Van Meerbeek; Yasuhiro Yoshida; Johan Snauwaert; Louis Hellemans; Paul Lambrechts; Guido Vanherle; Kunio Wakasa; David H. Pashleyg, Journal of Adhesive Dentistry, 1, 1, 7, 23, 1999
  English
• Effect of tannic acid solution on collagen structures for dental restoration
  Nurhayaty Natsir; K. Wakasa; Y. Yoshida; N. Satou; H. Shintani, Journal of Materials Science: Materials in Medicine, 10, 8, 489, 492, 1999
  Kluwer Academic Publishers, English
• Hybridization effectiveness of a two-step versus a three-step smear layer removing adhesive system examined correlatively by TEM and AFM
  Bart Van Meerbeek; Yasuhiro Yoshida; Johan Snauwaert; Louis Hellemans; Paul Lambrechts; Guido Vanherle; Kunio Wakasa; David H. Pashleyg, Journal of Adhesive Dentistry, 1, 1, 7, 23, 1999
  English
• A novel approach to AFM characterization of adhesive tooth-biomaterial interfaces
  Y. Yoshida; B. Van Meerbeek; J. Snauwaert; L. Hellemans; P. Lambrechts; G. Vanherle; K. Wakasa; D. H. Pashley, Journal of Biomedical Materials Research, 47, 1, 85, 90, 1999
  John Wiley & Sons Inc, English
• Effect of tannic acid solution on collagen structures for dental restoration
  Nurhayaty Natsir; K. Wakasa; Y. Yoshida; N. Satou; H. Shintani, Journal of Materials Science: Materials in Medicine, 10, 8, 489, 492, 1999
  Kluwer Academic Publishers, English
• Hybridization effectiveness of a two-step versus a three-step smear layer removing adhesive system examined correlatively by TEM and AFM
  Bart Van Meerbeek; Yasuhiro Yoshida; Johan Snauwaert; Louis Hellemans; Paul Lambrechts; Guido Vanherle; Kunio Wakasa; David H. Pashleyg, Journal of Adhesive Dentistry, 1, 1, 7, 23, 1999
  English
• A novel approach to AFM characterization of adhesive tooth-biomaterial interfaces
  Y. Yoshida; B. Van Meerbeek; J. Snauwaert; L. Hellemans; P. Lambrechts; G. Vanherle; K. Wakasa; D. H. Pashley, Journal of Biomedical Materials Research, 47, 1, 85, 90, 1999
  John Wiley & Sons Inc, English
• 象牙質接着性及び接着機序に関する基礎的研究(第12報) eigenstrainによるshrinkageの計算(micro-tensile bond testモデル)
  若狹 邦男; 吉田 靖弘; 池田 敦治; 山崎 保彦; 福田 竜一; 白井 憲一; 吉岡 雅之; 新谷 英章, 広島大学歯学雑誌, 30, 2, 243, 244, Dec. 1998
  広島大学歯学会, Japanese
• Dentine bonding system -Analysis of shrinkage at micro-tensile bond test-
  WAKASA K.; YOSHIDA Y.; IKEDA A.; YAMASAKI Y.; FUKUDA R.; SHIRAI K.; YOSHIOKA M.; SANO H., 歯科材料・器械, 17, 32, 172, 172, 30 Sep. 1998
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Dentine bonding system -Fracture model analysis of micro-tensile bond test-
  WAKASA K.; YOSHIDA Y.; IKEDA A.; YAMASAKI Y.; FUKUDA R.; SHIRAI K.; YOSHIOKA M.; YAMAKI M.; SANO H., 歯科材料・器械, 17, 31, 35, 35, 31 Mar. 1998
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Mechanisms of bonding of a resin-modified glass-ionomer adhesive to dentin.
  B Van Meerbeek; Y Yoshida; P Lambrechts; G Vanherle; K Wakasa; Y Nakayama, JOURNAL OF DENTAL RESEARCH, 77, 911, 911, 1998
  English, Summary international conference
• Adherent bacteria cells in five dental materials: Sonication effect
  Y. Yoshida; K. Wakasa; Y. Kajie; H. Takahashi; H. Urabe; N. Satou; H. Shintani; M. Yamaki, Journal of Materials Science: Materials in Medicine, 9, 2, 117, 120, 1998
  English
• Adherent bacteria cells in five dental materials: Sonication effect
  Y. Yoshida; K. Wakasa; Y. Kajie; H. Takahashi; H. Urabe; N. Satou; H. Shintani; M. Yamaki, Journal of Materials Science: Materials in Medicine, 9, 2, 117, 120, 1998
  English
• 象牙質接着システム 微小な被着面と接着強さとの関係(micro-tensile bond test計算モデル)
  若狹 邦男; 吉田 靖弘; 池田 敦治; 白井 憲一; 吉岡 雅之; 松井 昌; 山木 昌雄; 佐野 英彦, 歯科材料・器械, 16, 特別30, 31, 31, Sep. 1997
  (一社)日本歯科理工学会, Japanese
• A Fundamental Study on Dental Application of Silver Zeolite : Dental Restorative Materials
  Wakasa, Kunio; Yoshida, Yasuhiro; Nomura, Yuuji; Ikeda, Atsuharu; Nakatsuka, Akio; Ogino, Sekiya; Matsui, Hideki; Shirai, Ken-ichi; Yoshioka, Masayuki; Yamaki, Masao, 広島大学歯学雑誌, 29, 1, 87, 98, Jun. 1997
  銀配合量の多いAJ10Nはそれらの材質を銀イオンによって変色させるので,有効でない.銀配合量の少ないAW10N, SW80Nを用いるとその配合量(1～2wt%)によっては銀イオンの溶出が生じるので用いた材料の材質劣化が少ない.銀ゼオライト添加歯冠修復材料が抗う蝕性材料として有用な素材であり,更に接着レジンに応用した場合,結果的にそれらのレジンに含まれることになる金属イオン成分がレジン/象牙質界面の所要材質に与える影響も有効であると思われる, 広島大学歯学会, Japanese
• Dentine bonding system -Resin/dentine interface model-
  WAKASA K.; YOSHIDA Y.; IKEDA A.; SHIRAI K.; YOSHIOKA M.; YAMAKI M.; MATSUI A., 歯科材料・器械, 16, 29, 98, 98, 20 Mar. 1997
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Calculation of stress zones during crack propagation.
  K Wakasa; Y Yoshida; B Ciucchi; H Sano; DH Pashley, JOURNAL OF DENTAL RESEARCH, 76, 199, 199, 1997
  English, Summary international conference
• A TEM study of two water-based adhesive systems bonded to dry and wet dentin.
  B VanMeerbeek; P Lambrechts; G Vanherle; ES Duke; JD Eick, JOURNAL OF DENTAL RESEARCH, 76, 50, 50, 1997
  English, Summary international conference
• A TEM study of two water-based adhesive systems bonded to dry and wet dentin.
  B VanMeerbeek; P Lambrechts; G Vanherle; ES Duke; JD Eick, JOURNAL OF DENTAL RESEARCH, 76, 1, 50, 50, 1997
  English, Summary international conference
• Dental application of polyfunctional urethane comonomers to composite resin veneering materials
  K. Wakasa; Y. Yoshida; A. Ikeda; Nurhayaty Natsir; N. Satou; H. Shintani; M. Yamaki, Journal of Materials Science: Materials in Medicine, 8, 1, 57, 60, Jan. 1997
  Chapman & Hall Ltd, English
• A TEM study of two water-based adhesive systems bonded to dry and wet dentin.
  B VanMeerbeek; P Lambrechts; G Vanherle; ES Duke; JD Eick, JOURNAL OF DENTAL RESEARCH, 76, 1, 50, 50, 1997
  English, Summary international conference
• Dental application of polyfunctional urethane comonomers to composite resin veneering materials
  K. Wakasa; Y. Yoshida; A. Ikeda; Nurhayaty Natsir; N. Satou; H. Shintani; M. Yamaki, Journal of Materials Science: Materials in Medicine, 8, 1, 57, 60, Jan. 1997
  Chapman & Hall Ltd, English
• Interfacial stress distribution between bovine dentine and resin composite in dentine bonding systems
  K. Wakasa; Y. Yoshida; A. Nakatsuka; A. Ikeda; M. Yamaki, Journal of Materials Science: Materials in Medicine, 7, 10, 617, 621, Oct. 1996
  English
• Interfacial stress distribution between bovine dentine and resin composite in dentine bonding systems
  K. Wakasa; Y. Yoshida; A. Nakatsuka; A. Ikeda; M. Yamaki, Journal of Materials Science: Materials in Medicine, 7, 10, 617, 621, Oct. 1996
  English
• Dentine bonding system -Smear layer model-
  WAKASA K.; YOSHIDA Y.; IKEDA A.; YAMAKI M.; MATSUI A., 歯科材料・器械, 15, 28, 166, 167, 05 Sep. 1996
  接着領域(bonding area)と象牙質樹脂含浸層(hybrid layer)に注目し, しかもsmear layerが応力とひずみの挙動に影響する因子を調べるため, まずはじめにそれらlayerを考慮した応力解析モデルを創案し, その物性, ポアソン比や弾性率の役割を明らかにすることに応用した。次に推定する物性のうちsmear layerの大きさと厚さの影響を明らかにし, このモデルはsmear layerでの応力計算に有効であることがわかった。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Dentine bonding system Fracture stress analysis model
  WAKASA K.; YOSHIDA Y.; IKEDA A.; YAMAKI M.; MATSUI A., 歯科材料・器械, 15, 27, 144, 145, 28 Mar. 1996
  象牙質接着システムでは象牙質・レジン界面層, とくに接着領域(bonding area)と象牙質樹脂含浸層(hybrid layer)での応力の発生はその変形挙動に依存するため, まずはじめに破壊解析モデルを創成し, その物性, ポアソン比や弾性率を計算することに応用した。その結果, 推定する物性のうち接着領域の大きさと接着領域厚さの影響が明らかになり, このモデルは接着領域での破壊応力計算に有効であった。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Dental castable glass ceramics: ceramming treatment and colour property
  K. Wakasa; A. Ikeda; Y. Yoshida; M. Yamaki, Journal of Materials Science: Materials in Medicine, 6, 1, 29, 31, May 1995
  Kluwer Academic Publishers, English
• Dental castable glass ceramics: ceramming treatment and colour property
  K. Wakasa; A. Ikeda; Y. Yoshida; M. Yamaki, Journal of Materials Science: Materials in Medicine, 6, 1, 29, 31, May 1995
  Kluwer Academic Publishers, English
• Dentine bonding system -Stress analysis of composite resin (filler morphology)-
  WAKASA K.; IKEDA A.; YOSHIDA Y.; YAMAKI M.; MATSUI A., 歯科材料・器械, 14, 25, 182, 183, 31 Mar. 1995
  象牙質接着システムとして, 象牙質・レジン界面層や接着領域(bonding area)を含む計算モデルにもとづいて, それらレジン側での応力modelおよび内部応力問題から, composite resin側での応力をfiller morphologyの関係から検討して, 推定したfillerの形態から応力増加(降伏後)について明らかにすることができた。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Dentine bonding system : Dentine・Resin interface, especially, stress analysis of composite resin sides
  WAKASA K.; IKEDA A.; YOSHIDA Y.; YAMAKI M.; MATSUI A., 歯材器, 13, 42, 43, 1994
  象牙質接着性・接着機構に関する基礎的研究として, 象牙質・レジン界面層(以下, 界面層とする)を含む試料の接着性評価にもとづいて, 計算モデルをつくり, それらの変形挙動を評価した。さらに界面層を介して, そのコンポジットレジン側の応力を, その計算モデルから求めた。その結果から, レジンの変形挙動は界面を含む試料の接着性試験での変形挙動とよく対応することを明確にした。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Dentine bonding system : Deformation of the bonding area and its stress analysis
  WAKASA K.; IKEDA A.; YOSHIDA Y.; YAMAKI M.; MATSUI A., 歯材器, 13, 272, 273, 1994
  象牙質接着性を明らかにするため, 象牙質・レジン界面層(以下, 界面層とする), あるいは接着領域(bonding area)について, 計算モデルをつくり, それらの変形挙動を評価した。nano-indentation testによる硬さと界面層の厚さとの関係を求め, 次に, 界面層の変形について計算モデルを創成して, 界面層(あるいは接着領域)の変形挙動と界面のelasticityや塑性変形領域の関連性を明らかにした。, The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• Ethyl silicate-bonded investments: Effect of silica gel as a binder on DSC results
  K. Wakasa; M. Yamaki, Journal of Materials Science Letters, 13, 16, 1177, 1178, Jan. 1994
  Kluwer Academic Publishers, English
• Effect of gelation on setting time in ethyl silicate-bonded investment
  K. Wakasa; Y. Yoshida; A. Ikeda; M. Yamaki, Journal of Materials Science Letters, 13, 4, 258, 259, Jan. 1994
  Kluwer Academic Publishers, English
• Ethyl silicate-bonded investments: Effect of silica gel as a binder on DSC results
  K. Wakasa; M. Yamaki, Journal of Materials Science Letters, 13, 16, 1177, 1178, Jan. 1994
  Kluwer Academic Publishers, English
• Effect of gelation on setting time in ethyl silicate-bonded investment
  K. Wakasa; Y. Yoshida; A. Ikeda; M. Yamaki, Journal of Materials Science Letters, 13, 4, 258, 259, Jan. 1994
  Kluwer Academic Publishers, English
• Effect of Denture Cleanser on Properties in Denture Base Resin : (Part 4) pH Value of Denture Cleanser and Colour Change Vector of Denture Base Materials
  Wakasa, Kunio; Hirose, Tomoji; Taira, Masayuki; Nomura, Yuuji; Tsuji, Takeshi; Ikeda, Atsuharu; Yoshida, Yasuhiro; Yamaki, Masao, 広島大学歯学雑誌, 25, 2, 489, 493, 01 Dec. 1993
  広島大学歯学会, Japanese
• Effect of Denture Cleanser on Properties in Denture Base Resin : (Part 3) Mechanical Properties in Polyethelsulphone Base Resin
  Wakasa, Kunio; Hirose, Tomoji; Taira, Masayuki; Nomura, Yuuji; Tsuji, Takeshi; Ikeda, Atsuharu; Yoshida, Yasuhiro; Yamaki, Masao, 広島大学歯学雑誌, 25, 1, 212, 216, 06 May 1993
  広島大学歯学会, Japanese
• A Case Report of Complete Denture Treatment with Patient's Satisfaction
  Akagawa, Yasumasa; Yoshida, Yasuhiro; Sakurai, Hiroya; Habu, Eisaku; Sato, Yuuji; Tsuru, Hiromichi, 広島大学歯学雑誌, 24, 1, 138, 144, 01 Jun. 1992
  広島大学歯学会, Japanese
• Mechanism of chemical bonding at toothpolycarboxylic acid interfaces
  FUKUDA R.; YOSHIDA Y.; NOMURA Y.; WAKASA K.; OKAZAKI M.; SHINTANI H.; VANMEERBEEK Bart, 歯科材料・器械, 18, 34, 56, 56, 25 Sep. 1990
  Japanese
• Molecule-level kinetics of organic acids on biological hard tissues
  YOSHIDA Y.; NOMURA Y.; WAKASA K.; OKAZAKI M.; YOSHIOKA M.; SHINTANI H.; VANMEERBEEK B., 歯科材料・器械, 18, 34, 57, 57, 25 Sep. 1990
  Japanese
• Dentine bonding system -Collagen fibre analysis of demineralized dentine at micro -tensile bond test-
  WAKASA K.; YOSHIDA Y.; IKEDA A.; YAMAZAKI Y.; FUKUDA R.; YOSHIOKA M.; NOMURA Y.; OKAZAKI M.; SANO H., 歯科材料・器械, 18, 34, 229, 229, 25 Sep. 1990
  Japanese
• Standies on dissolution of visible light-curing resin (part 2) Examination on urethane resin
  NOMURA Y.; MOINJAN C.; URABE H.; YOSHIDA Y.; WAKASA K.; SHINTANI H.; OKAZAKI M., 歯科材料・器械, 18, 34, 166, 166, 25 Sep. 1990
  Japanese
• Inhibitory effect and physical properties of dental glass-ionomer cements．
  広島大学歯学雑誌, 30, 23-27
• Two-dimensional FE analysis of principal stress within bonding area as resin composite/dentine interface（共著）
  広島大学歯学雑誌, 30, 8-12
• Thermal change in binary polyfunctional urethane monomer mixtures for visible light-cured resins（共著）
  広島大学歯学雑誌, 30, 1-7
• Factors influencing clinically successful bonding to dentin and enamel.(共著)
  American Journal of Dentistry
• 残存象牙質の厚みがレジン接着用光硬貨型グラスアイオノマーセメントの微小引張り接着強さに及ぼす影響.(共著)
  接着歯学, 18, 93-99
• Factores que influencian el exito clinico de la adhesion a la dentina y esmalte (II)(共著)
  Maxillaris, II(15), 20-30
• Factores que influencian el exito clinico de la adhesion a la dentina y esmalte (I)(共著)
  Maxillaris, II(14), 26-36
• Distortion analysis of casting mold in dental casting (2-D Finite element method)
  広島大学歯学雑誌, 30, 165-170
• Cristobalite-quartz investment for apatite-based glass ceramic (共著)
  27, 1, 10, 14
• Dental use of hydrolyzed solution in cast ethyl silicate-bonded investment (共著)
  27, 1, 7, 9
• Exothermic setting behaviour in dental investment and gypsum(Thermoanalytical method)(共著)
  26, 263, 266
• Thermoanalytical characterization of dental porcelains and kaolinite/feldspar minerals(共著)
  26, 197, 202
• Microscopy Investigations-Techniques, Results, Limitations(共著)
  American Journal of Dentistry, 13, 3D-18D
• Dental apatite glass ceramics-Sprue and dimension for ability to cast(共著)
  26, 188, 191
• Effect of Remaining Dentin Thickness on Micro-tensile Band Strength of a Resin-modified Glass-ionomer Cement for Bonding Composite Resin
  Adhesive Dentistry, 18, 2, 93, 99
• Factores que influencian el (]E85EE[)xito cl(]E85F1[)nico de la adhesi(]E85F6[)n a la dentina y esmaalte (y (]G0002[))(共著)
  Maxillaris, 2, 15/10, 20, 30
• Factores que influencian el (]E85EE[)xito cl(]E85F1[)nico de la adhesi(]E85F6[)n a la dentina y esmalte ((]G0001[)) (共著)
  Maxillaris, 2, 14/, 9,26-36
• Distortion analysis of casting moldin dental casting(2-D Finite element method)
  30, 168, 170
• Inhibitory effect and physical properties of dental glass-ionomer cements(共著)
  30, 23, 27
• Two-dimencional FE analysis of prineipal stress within bonding area as resin composite/dentine interface(共著)
  30, 8, 12
• Thermal change in binary polyfunctional urethane monomer mixtures for visible light-cured resins(共著)
  30, 1, 7
• Microscopy of resin-dentin interfaces(共著)
  Transactions of the Academy of Dental Materials, 11, 33, 63
• Developmental study of functional galss ceramics Part3 A Synthesis of binary CaO/P₂O₅ glass ceramics(共著)
  29, 207, 212
• Developmental study of functional glass ceramics Part2 DTA-TG analysis of powder mixtures applied for dental purpose(共著)
  29, 201, 206
• Developmental study of functional glass ceramics Part1 Strength evaluation(共著)
  29, 193, 200
• Dentine bonding : Effects of applied stress and thickness on principal stress within the resin composite/dentine interface(FEM analysis)(共著)
  29, 7, 11
• Titanium casting : Effect of mould condition of magnesia-based investment(共著)
  29, 1, 6
• Analytical model of shear and tensile bond testing : Definition of yield criteria(共著)
  28, 279, 282
• Accuracy of apatite-based glass ceramic in dental cast ethyl silicate-bonded investment : Theoretical consideration(共著)
  28, 5, 10
• Absorbance spectra analysis of photosensitizer and reducing agent for dental light-cured resin monomers (共著)
  27, 2, 365, 368
• Fibre distribution of light conductors in dental visible-light curing units (共著)
  27, 2, 359, 364
• Dental visible-ligth curing units : intensity characteristics and photopolymerization (共著)
  27, 2, 351, 358
• Surface roughness ralue in cast and cerammed apatite-based glass ceramic (共著)
  27, 1, 15, 18
• Cristobalite-quartz investment for apatite-based glass ceramic (共著)
  27, 1, 10, 14
• Dental use of hydrolyzed solution in cast ethyl silicate-bonded investment (共著)
  27, 1, 7, 9
• Exothermic setting behaviour in dental investment and gypsum(Thermoanalytical method)(共著)
  26, 263, 266
• Thermoanalytical characterization of dental porcelains and kaolinite/feldspar minerals(共著)
  26, 197, 202
• Microscopy Investigations-Techniques, Results, Limitations(共著)
  American Journal of Dentistry, 13, 3D-18D
• Dental apatite glass ceramics-Sprue and dimension for ability to cast(共著)
  26, 188, 191
• Effect of Remaining Dentin Thickness on Micro-tensile Band Strength of a Resin-modified Glass-ionomer Cement for Bonding Composite Resin
  Adhesive Dentistry, 18, 2, 93, 99
• Factores que influencian el (]E85EE[)xito cl(]E85F1[)nico de la adhesi(]E85F6[)n a la dentina y esmaalte (y (]G0002[))(共著)
  Maxillaris, 2, 15/10, 20, 30
• Factores que influencian el (]E85EE[)xito cl(]E85F1[)nico de la adhesi(]E85F6[)n a la dentina y esmalte ((]G0001[)) (共著)
  Maxillaris, 2, 14/, 9,26-36
• Distortion analysis of casting moldin dental casting(2-D Finite element method)
  30, 168, 170
• Inhibitory effect and physical properties of dental glass-ionomer cements(共著)
  30, 23, 27
• Two-dimencional FE analysis of prineipal stress within bonding area as resin composite/dentine interface(共著)
  30, 8, 12
• Thermal change in binary polyfunctional urethane monomer mixtures for visible light-cured resins(共著)
  30, 1, 7
• Microscopy of resin-dentin interfaces(共著)
  Transactions of the Academy of Dental Materials, 11, 33, 63
• Developmental study of functional galss ceramics Part3 A Synthesis of binary CaO/P₂O₅ glass ceramics(共著)
  29, 207, 212
• Developmental study of functional glass ceramics Part2 DTA-TG analysis of powder mixtures applied for dental purpose(共著)
  29, 201, 206
• Developmental study of functional glass ceramics Part1 Strength evaluation(共著)
  29, 193, 200
• Dentine bonding : Effects of applied stress and thickness on principal stress within the resin composite/dentine interface(FEM analysis)(共著)
  29, 7, 11
• Titanium casting : Effect of mould condition of magnesia-based investment(共著)
  29, 1, 6
• Analytical model of shear and tensile bond testing : Definition of yield criteria(共著)
  28, 279, 282
• Accuracy of apatite-based glass ceramic in dental cast ethyl silicate-bonded investment : Theoretical consideration(共著)
  28, 5, 10
• Absorbance spectra analysis of photosensitizer and reducing agent for dental light-cured resin monomers (共著)
  27, 2, 365, 368
• Fibre distribution of light conductors in dental visible-light curing units (共著)
  27, 2, 359, 364
• Dental visible-ligth curing units : intensity characteristics and photopolymerization (共著)
  27, 2, 351, 358
• Surface roughness ralue in cast and cerammed apatite-based glass ceramic (共著)
  27, 1, 15, 18

• Photochemistry for Biomedical Applications: From Device Fabrication to Diagnosis and Therapy
  Kumiki Yoshihara; Yasuhiro Yoshida, Dental Resin
  Springer, 05 Jul. 2018, 9789811301513, 321, 179-198, [Contributor]
• 生体吸収性材料の開発と安全性評価
  執筆者:103名, 第4節 歯科領域で求めるコンビネーションプロダクト製品像, 第8章 コンビネーションプロダクト（医薬品・生体吸収性材料）の臨床での使われ方と安全性, 生体吸収性
  技術情報協会, 01 Jan. 2017, 4861046890, 465p, Japanese
• Caries management : science and clinical practice
  Van Meerbeek, B.; Yoshida, Y., Basics in adhesion technology
  Thieme Medical Publishers, Inc., Stuttgart, Germany, 2013, 9783131547118, xxi, 414 p., English, [Contributor]
• Wissenschaft und Klinische Praxis
  Van Meerbeek, B.; Yoshida, Y., Grundlagen der Adhäsivtechnik: In Karies
  Georg Thieme Verlag, Stuttgart, Germany, 2012, 9783131545411, 232-248, German, [Contributor]
• Phosphorylated pullulan bioadhesive for regeneration and reconstruction of bone and tooth.
  Yoshida, Y.; Okihara, T.; Nakamura, M.; Matsumoto, T.
  Key Engineering Materials, 2012, 529-530, [Joint work]
• Visible Light-Induced Crosslinkable Gelatin for Direct Pulp Capping
  Nakamura, M.; Yoshida, Y.; Ito, Y.
  Key Engineering Materials, 2012, 543-546, [Joint work]
• スタンダ-ド歯科理工学: 生体材料と歯科材料
  吉田靖弘
  鍬谷書店, 20 Mar. 2009, 4762426148, 363, Japanese, [Contributor]
• Statements: Diagnostics and Therapy in Dental Medicine Today and in the Future
  Cardoso, M.V.; Yoshida, Y.; Van Meerbeek, B., Adhesion to tooth enamel and dentin – A view on the latest technology and future perspectives
  Quintessence Pub Co, 30 Nov. 2008, 9781850971825, 245, 25-43, [Contributor]
• スタンダード歯科理工学 : 歯科生体材料・歯科材料
  吉田靖弘
  学建書院, Mar. 2007, 9784762416149, 401p, Japanese, Textbook, [Contributor]
• Cell proliferation on Titania layer with in vitro apatite forming ability
  Yabe, S.; Tsuru, K.; Hayakawa, S.; Osaka, A.; Yoshida, Y.; Suzuki, K.; Kuboki, T.
  Key Engineering Materials, 2007, 131-134, [Joint work]
• 日本歯科評論別冊／接着性コンポジットレジン修復の基礎と臨床
  ヒョーロン・パブリッシャーズ,東京, 2007
• Fundamentals of operative dentistry : a contemporary approach
  Van Meerbeek, B.; Van Landuyt, K.; De Munck, J.; Inoue, S.; Yoshida, Y.; Perdigão, J.; Lambrechts, P.; Peumans, M., Bonding to Enamel and Dentin
  Quintessence Pub., 31 Jan. 2006, 0867154527, 599, 182-260, English, [Contributor]
• 歯界展望別冊 臨床に役立つ接着修復のすべて
  医歯薬出版,東京, 2006
• 歯科理工学教育用語集
  日本歯科理工学会
  医歯薬出版, Sep. 2005, 9784263455852, 158p, Japanese
• Proceeding of International Symposium for Interface Oral Health Science in Sendai
  Maekawa, K.; Mine, A.; Fujisawa, T.; Yoshida, Y.; Suzuki, K.; Kuboki T., Adsorption of polyphosphoric acid to titanium surface and its effect on hBMSC attachment
  Elsevier Science B.V.,Amsterdam, 2005, 332-333, [Joint work]
• 平成14-15年度科学研究費補助金（基盤研究(C)(2)）研究成果報告書
  2004
• 上原記念生命科学財団研究報告集 Vol.18
  上原記念生命科学財団,東京, 2004
• (財)中冨健康科学振興財団第15回研究助成業績集
  中冨健康科学振興財団,東京, 2004
• Expanded Horizon in Advanced Materials and Processing Vol 2
  JISSE-8,Tokyo, 2003
• Archives of BioCeramics Research Volume 3
  Yamasaki, Y.; Yoshida, Y.; Hirata, I.; Shimazu, M.; Okazaki, M., Osteoblast adhesion behavior onto functionally graded MgCO3 apatite
  サガプリンティング,佐賀, 2003, 330-335, [Joint work]
• Advanced Materials and Processing Vol 2
  Yoshida, Y.; Van Meerbeek, B.; Suzuki, K., Current status of dental adhesive technology
  2003, 1221-1224, [Joint work]
• Archives of BioCeramics Research Volume 3
  Shibata, S.; Shirai, K.; Shintani, T.; Itoh, M.; Motoyama, T.; Yoshida, Y.; Suzuki, K.; Okazaki, M.; Shintani, H., Filler Decontamination Methods Improving Degradation of Resin Composites
  2003, 57-62, [Joint work]
• Archives of BioCeramics Research Volume 3
  Fukuda, R.; Yoshida, Y.; Van Meerbeek, B.; Okazaki, M.; Suzuki, M.; Shintani, H., Chemical bonding potential of polyalkenoic acids to enamel and dentin
  2003, 89-94, [Joint work]
• Tissue Engineering for Therapeutic Use 6
  Yoshida, Y.; Okazaki, M.; Van Meerbeek, B., Chemical interaction of acids with apatitic substrates.
  Elsevier Science B.V.,Amsterdam, 2002, 161-168, [Joint work]
• Peptide Science 2000
  Nokihara K.; Blahunka A.; Yamazaki Y.; Yoshida Y.; Okazaki M., Development of biomimetic materials: Novel composite material carring immobileized functional peptides
  The Japanese Peptide Society, 2001, 373-376
• SELF-Etching PRIMER: Current Status and Its Evolution
  Van Meerbeek, B.; Yoshida, Y.; De Munck J.; Inoue S.; Vargas, M.; Lambrechts P.; Vanherle G., Correlative morphologic and chemical interfacial characterrization of tooth-biomaterial interactions
  2001, 41-55, [Joint work]
• 8th International Conference on Composites Engineering ICCE/8
  Inoue, S.; Van Meerbeek, B.; Yoshida, Y.; Sano, H., Adhesion mechanism and bonding effectiveness of a glass-ionomer adhesive for resin composite restoration
  2001, B11-B14
• Advanced Adhesive Dentistry 3rd International Kuraray Symposium
  Inoue, S.; Van Meerbeek, B.; Vargas, M.; Yoshida, Y.; Lambrechts, P.; Vanherle, G., Adhesion mechanisms of self-etching adhesives
  Kuraray Co. Ltd, 2000, 131-148
• 4th European Conference on Composites: Testing and Standarisation
  Wakasa, K.; Yoshida, Y.; Shintani, H., Interface modeling of micro-tensile bond test
  1998, 253-261, [Joint work]
• Computer Methods in Biomechanics & Biomedical Engineering – 2
  Wakasa, K.; Yoshida, Y.; Nakayama, Y.; Nakatsuka, A.; Yamaki, M., Stress analysis after yielding at the resin composite/dentine interface
  1998, 673-680, [Joint work]
• Sixth Annual International Conference on Composites Engineering ICCE/6
  Wakasa, K.; Yoshida, Y., Fracture analysis of resin composite/dentine interfaces
  1998, 865-866, [Joint work]
• 5th International Conference on Composites Engineering ICCE/5
  Wakasa, K.; Yoshida, Y., Theoretical calculation model of micro-tensile bond test
  1998, 925-926, [Joint work]
• Seventh European Conference on Composite Materials ECCM-7
  Wakasa, K.; Yoshida, Y.; Shintani, H.; Yamaki M., Fracture process of resin composite/dentine adhesive interfaces
  Novel biomaterials in prosthodontics and implantology and clinical relevent aspects on occlusion, 1993, 467-474, [Joint work]
• Analytical model of fraeture at the dentine/resin composite interface (共著)
  ICCE/3, ICCE & Coll. of Eng, Univ. NO
• Fracture process of resin composite/dentine adhesive interfaces(共著)
  Novel biomaterials in prosthodontics and implantology and clinical relevent aspects on occlusion

• A Novel alveolar bone graft using polysaccharide derivative 'Phosphorylated Pullulan' for autologous bone augmentation material
  NAKANISHI K.; TAMADA I.; NAKAMURA M.; YOSHIDA Y.; SATO Y.
  The 84th Annual Meeting of the Japanese Orthodontic Society, 30 Sep. 2025
  29 Sep. 2025 - 01 Oct. 2025
• マイクロ・ナノパターン上でのヒト乳歯歯髄幹細胞の挙動
  赤坂 司; 中西 康; 吉田靖弘
  第82 回日本歯科理工学会学術講演会, 20 Apr. 2024
  20 Apr. 2024 - 21 Apr. 2024
• 思い込みを捨てよう！若手研究者と創造する未来歯科医療
  吉田 靖弘
  公益社団法人 日本補綴歯科学会 Prosthodontic Meeting for Next Generation 2024, 29 Mar. 2024, Invited oral presentation
• 思い込みを捨てよう！若手が創る未来歯科医療
  吉田 靖弘
  北海道医療大学歯学会42回 学術大会, 02 Mar. 2024, Invited oral presentation
• 大型研究事業の企画と提案書作成のコツ
  吉田 靖弘
  一般社団法人 日本歯科医学会連合 第7回大型医療研究フォーラム, 17 Feb. 2024, Invited oral presentation
• 世界トップレベルの歯科材料・機器を臨床につなげる！〜若手研究者の自由な発想と努力がもたらす大型医療研究事業〜
  吉田 靖弘
  令和５年度 公益社団法人 日本補綴歯科学会 東北・北海道支部 学術大会, 05 Oct. 2023, Invited oral presentation
• 非動物由来の生体吸収性ポリマーを用いた骨再生・再建
  吉田 靖弘
  第53回 公益社団法人 日本口腔インプラント学会 学術大会, 17 Sep. 2023, Invited oral presentation
• Future dentistry with antibacterial materials and implantable absorbable materials that adhere to living tissues
  Yasuhiro Yoshida
  Biomaterials International 2023, 31 Jul. 2023, Invited oral presentation
• 臨床へ実装されるバイオロジー研究 〜研究室から診療室へ〜
  吉田 靖弘
  日本補綴歯科学会 132回 学術大会, 20 May 2023, Invited oral presentation
• 破骨細胞分化へのパターン材質と前駆細胞種の影響
  赤坂 司; 中西 康; 吉田靖弘
  第81 回日本歯科理工学会学術講演会, 15 Apr. 2023
  15 Apr. 2023 - 16 Apr. 2023
• ものづくり研究の進め方 －大型研究事業の企画立案と申請書作成のコツ
  吉田 靖弘
  第35回代用臓器・再生医学研究会総会／日本バイオマテリアル学会北海道ブロック第7回研究会, 18 Feb. 2023, Invited oral presentation
• Non-animal-derived bioabsorbable polymer for bone regeneration
  Nakanishi K; Akasaka T; Yoshihara K; Nakamura M; Yoshida Y
  INTERNATIONAL DENTAL MATERIALS CONGRESS, 04 Nov. 2022
• 北海道大学が進めるデンタルイノベーション
  吉田 靖弘
  2022年度東北大学金属材料研究所共同研究ワークショップ 日本バイオマテリアル学会東北ブロック講演会, 26 Oct. 2022, Invited oral presentation
• 大型医療研究の進め方 － 歯科における「ものづくり事業」の具体例
  吉田 靖弘
  第6回大型医療研究推進フォーラム, 22 Aug. 2022
• 抗菌性ポリウレタンゲル粘着シートの塩化セチルピリジニウム徐放特性と粘着性能
  中守貴一; 阿部泰彦; 吉原久美子; 吉田靖弘; 津賀一弘
  第79回日本歯科理工学会学術講演会, 21 May 2022
• 研究力の指標となる論文業績アップとイノベーションによる製品開発の両立
  吉田 靖弘
  URAステーション, 16 May 2022
• 微細構造が破骨細胞への分化に与える影響
  赤坂 司、中西 康、吉田 靖弘
  第 34 回代用臓器・再生医学研究会総会 日本バイオマテリアル学会北海道ブロック第 6 回研究会, 19 Feb. 2022
• A novel bioabsorbable polysaccharide derivative for minimally invasive bone graft harvesting
  Nakanishi K, Hasegawa T, Hayashi H, Nagamoto K, Oguma H, Akasaka T, Yoshida Y
  The 69th Annual Meeting of Japanese Association for Dental Research
  24 Oct. 2021 - 25 Oct. 2021
• 北海道大学におけるデンタルイノベーション戦略
  吉田 靖弘
  第78回日本歯科理工学会, 16 Oct. 2021, Nominated symposium
• 次世代の歯質接着治療に向けた機能性分子の設計理論構築
  小熊英敏; 中西康; 吉原久美子; 赤坂司; 松田康裕; 吉田靖弘; 井上哲
  令和3年度日本歯科理工学会北海道・東北地方会夏季セミナー, 21 Aug. 2021
• 歯科用コンビネーションプロダクトの実用化に向けた取り組み
  吉田靖弘; 吉原久美子; 長本香菜子; 中西 康; 赤坂 司; 阿部泰彦; 横山敦郎
  令和3年度日本歯科理工学会北海道・東北地方会夏季セミナー, 21 Aug. 2021, Invited oral presentation
• 接着性生体吸収材料を用いたインプラント治療のための新骨移植法
  長本香菜子、中西康、中村真理子、小熊英敏、赤坂司、吉田靖弘
  令和3年度日本歯科理工学会北海道・東北地方会夏季セミナー, 21 Aug. 2021
• Effect Of Polymerization On Additive Manufacturing Of Zirconia Ceramics
  Yoshihara K; Shimizu T; Nagaoka N; Yoshida Y; Van Meerbeek B, Kirihara S
  2021 IADR/AADR/CADR General Session, 21 Jul. 2021
• 破骨細胞の吸収窩形成へのマイクロ・ナノパターンの影響
  赤坂 司; 中西 康; 吉田靖弘
  第77回日本歯科理工学会
  10 Apr. 2021 - 11 Apr. 2021
• マイクロナノパターンが骨系細胞に与える影響について
  沼本真一郎; 横山敦郎; 赤坂司; 吉田靖弘
  第33回代用臓器・再生医科研究会総会, 27 Feb. 2021
• 破骨細胞分化誘導へのマイクロパターンの影響
  赤坂司; 玉井美保; 吉田靖弘
  第75回日本歯科理工学会学術公演会
  18 Apr. 2020 - 19 Apr. 2020
• 矯正歯科用ステンレススチールワイヤーへのイオン溶出抑制を目的とした表面化学修飾
  玉木 宏典; 阿部 薫明; 山方 秀一; 佐藤 嘉晃; 飯田 順一郎; 吉田 靖弘
  第78回日本矯正歯科学会学術大会, Japanese
  20 Nov. 2019 - 22 Nov. 2019
• リン酸三カルシウム（TCP）およびリン酸化プルラン(PPL)含有新規骨補填材の開発
  森本康仁; 長谷川智香; 本郷裕実; 吉田靖弘; 網塚憲生; 菅谷勉
  第62回秋季日本歯周病学会学術大会
  24 Oct. 2019 - 26 Oct. 2019
• ユニバーサルボンディング材のI級窩洞における象牙質接着強さ
  吉原 久美子; 長岡 紀幸; 吉田 靖弘
  日本歯科保存学会2019春季学術大会（第150回）, Japanese
  27 Jun. 2019 - 28 Jun. 2019
• リン酸化プルラン含有MTAシーラーが実験的根尖性歯周炎の治癒に及ぼす効果
  河野 通俊; 大嶌 理紗; 中塚 愛; 中谷 充宣; 前田 良子; 下地 伸司; 菅谷 勉; 吉田 靖弘
  日本歯科保存学会2019春季学術大会（第150回）, Japanese
  27 Jun. 2019 - 28 Jun. 2019
• 核反応を利用したフッ化物含有接着材からの歯質内フッ素分布の測定
  奥山 克史; 松田 康裕; 山本 洋子; 新谷 耕平; 堀口 敬司; 斉藤 隆史; 林 美加子; 吉田 靖弘; 玉置 幸道
  第73回日本歯科理工学会学術公演会, Japanese
  20 Apr. 2019 - 21 Apr. 2019
• カゴ状ナノシリカ粒子を用いた薬剤徐放性歯科材料の開発
  江良 裕子; 阿部 薫明; 赤坂 司; 吉田 靖弘
  第73回日本歯科理工学会学術公演会, Japanese
  20 Apr. 2019 - 21 Apr. 2019
• Groove/Pillarパターン化チタン表面が細胞接着及び増殖に与える影響
  加我 公行; 赤坂 司; 吉田 靖弘
  第73回日本歯科理工学会学術公演会, Japanese
  20 Apr. 2019 - 21 Apr. 2019
• 液体アクリルアミド系モノマー含有レジン添加型覆髄材の検討
  吉原 久美子; 長岡 紀幸; 吉田 靖弘
  第73回日本歯科理工学会学術公演会, Japanese
  20 Apr. 2019 - 21 Apr. 2019
• リン酸三カルシウムTCP）およびリン酸化プルラン(PPL)含有新規骨補填剤による新生骨誘導における組織学的解析
  森本康仁; 長谷川智香; 邱紫せん; 松本愛子; 桐越晶子; 森谷康人; 橋本圭司; 趙申; 前壮功仁; 本郷裕実; 菅谷勉; 吉田靖弘; 網塚憲生
  第124回日本解剖学会総会・全国学術集会
  27 Mar. 2019 - 29 Mar. 2019
• ナノポーラスシリカを用いた新規な薬剤徐放能性歯科材料の開発
  江良 裕子; 成徳 英理; 中西 康; 坂東 洋祐; 阿部 薫明; 吉田 靖弘
  第31回代用臓器・再生医科研究会総会, 16 Feb. 2019, Japanese
• マイクロ・ナノパターン表面構造における細胞応答
  加我 公行; 赤坂 司; 吉田 靖弘; 横山 敦郎
  第31回代用臓器・再生医科研究会総会, 16 Feb. 2019, Japanese
• マイクロ・サブミクロパターン構造を付与したCOP film上における細胞挙動
  本間 淳; 赤坂 司; 山方 秀一; 佐藤 嘉晃; 吉田 靖弘
  平成30年度北海道大学歯学会秋季学術大会, 01 Dec. 2018, Japanese
• 化学重合型マルチプライマーの歯質接着界面の解析
  吉原久美子; 長岡紀幸; 吉田靖弘
  第72回日本歯科理工学会学術公演会
  06 Oct. 2018 - 07 Oct. 2018
• ワンステップ接着システムにおける歯質界面の生成物と物性の関係
  鈴木一臣; 長岡紀幸; 吉原久美子; 松本卓也; 吉田靖弘
  第72回日本歯科理工学会学術公演会
  06 Oct. 2018 - 07 Oct. 2018
• プライマー接触硬化型レジンセメント重合のリアルタイム測定
  吉原久美子; 長岡紀幸; 吉田靖弘
  第71回日本歯科理工学会学術公演会
  14 Apr. 2018 - 15 Apr. 2018
• ナノ多孔質シリカを用いた新規多機能性グラスアイオノマーセメントの開発
  坂東洋祐; 中西康; 山方秀一; 飯田順一郎; 阿部薫明; 吉田靖弘
  第30回代用臓器・再生医科研究会総会, 24 Feb. 2018, Japanese
• 北大歯におけるイノベーション・ベンチャー戦略
  吉田靖弘
  第30回代用臓器・再生医科研究会総会, 24 Feb. 2018, Japanese, Invited oral presentation
• マイクロ・ナノ構造が歯周組織関連細胞の挙動にもたらす影響
  高田 亮; 赤坂 司; 山方 秀一; 佐藤 嘉晃; 吉田 靖弘
  第30回代用臓器・再生医科研究会総会, 24 Feb. 2018, Japanese
• 薬剤徐放能を持つ多孔質シリカを用いた歯科用材料の開発
  江良裕子; 坂東洋祐; 中西康; 阿部薫明; 玉井美保; 赤坂司; 飯田順一郎; 吉田靖弘
  化学系学協会北海道支部冬季研究発表会(Web), 2018, Japanese
• 生体硬組織への接着技術を活かした医療イノベーション
  吉田靖弘
  第37回整形外科バイオマテリアル研究会, 02 Dec. 2017, Japanese, Invited oral presentation
• レジンセメントの曲げ強さに対する長期水中保管の影響
  吉原久美子; 長岡紀幸; 入江正郎; 吉田靖弘
  第36回日本接着歯学会学術大会, Japanese
  25 Nov. 2017 - 26 Nov. 2017
• ナノ構造体シリカからの薬剤徐放挙動と生体適合性の評価
  阿部薫明; 阿部薫明; 中西康; 中西康; 坂東洋祐; 成徳英理; 江良裕子; 飯田順一郎; 飯田順一郎; 佐野英彦; 佐野英彦; 吉田靖弘; 吉田靖弘
  第39回日本バイオマテリアル学会大会, Japanese
  20 Nov. 2017 - 21 Nov. 2017
• アルカリ環境が修復材料の耐久性に与える影響
  吉原久美子; 長岡紀幸; 小河達之; 吉田靖弘
  日本歯科保存学会2017秋季学術大会（第147回）, Japanese
  26 Oct. 2017 - 27 Oct. 2017
• Controlled release property of drug models on glass ionomer cement containing nanoporous materials
  江良裕子; 中西康; 中西康; 阿部薫明; 玉井美保; 赤坂司; 吉田靖弘
  第70回日本歯科理工学会学術公演会, Japanese
  14 Oct. 2017 - 15 Oct. 2017
• Micro/nano-patterning of gelatin by crosslinking with genipin
  赤坂司; 阿部薫明; 吉田靖弘
  第70回日本歯科理工学会学術公演会, Japanese
  14 Oct. 2017 - 15 Oct. 2017
• 異なる種類のフィラーが1液型ボンディング材の粘性,耐久性に与える影響
  吉原久美子; 小河達之; 長岡紀幸; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 08 May 2017, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーの根尖封鎖性
  大嶌理紗; 下地伸司; 菅谷勉; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 08 May 2017, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーによる根尖孔への硬組織形成
  河野通俊; 大嶌理紗; 熊谷広道; 佐藤賢人; 工藤愛; 中谷充宣; 前田良子; 大歳祐生; 下地伸司; 菅谷勉; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 08 May 2017, Japanese
• Micro/nano-patterning of curable dental materials
  赤坂司; 加我公行; 横山敦郎; 阿部薫明; 吉田靖弘
  日本歯科理工学会誌, 25 Mar. 2017, Japanese
• The development of acrylamide monomer containing pulp capping materials
  吉原久美子; 長岡紀幸; 吉田靖弘
  日本歯科理工学会誌, 25 Mar. 2017, Japanese
• 歯科矯正用ステンレススチールワイヤーの化学修飾による金属イオン溶出抑制
  土屋聡; 阿部薫明; 山方秀一; 吉田靖弘; 飯田順一郎
  日本矯正歯科学会大会プログラム・抄録集, 2017, Japanese
• 10‐MDPプライマー処理前の洗浄がジルコニアの接着に及ぼす影響
  吉原久美子; 長岡紀幸; 丸尾幸憲; 吉田靖弘
  日本補綴歯科学会誌(Web), 2017, Japanese
• ヒト由来細胞に対するセラミックナノ粒子の生体適合性の検討
  阿部薫明; 成徳英理; 江良裕子; 吉田靖弘; 佐野英彦
  日本バイオマテリアル学会シンポジウム予稿集, 21 Nov. 2016, Japanese
• Cytocompatibility of several nano-sized ceramics particles: In vitro study
  Y. Era; S. Abe; Y. Nakagawa; A. Hyono; M. Nakamura; Y. Yoshida
  The 17th Chitose International Forum on Photonics Science and Technology
  14 Nov. 2016 - 15 Nov. 2016
• 接着歯学の基礎研究が生んだセラピューティック・イノベーション
  吉田靖弘
  日本接着歯学会学術大会講演集(Web), 15 Nov. 2016, Japanese
• ジルコニアに対するカップリングモノマーの吸着特性
  長岡紀幸; 吉原久美子; 吉田靖弘
  日本接着歯学会学術大会講演集(Web), 15 Nov. 2016, Japanese
• 2ステップセルフエッチングボンディング材の長期臨床症例の接着界面観察
  吉原久美子; 長岡紀幸; 吉田靖弘
  日本接着歯学会学術大会講演集(Web), 15 Nov. 2016, Japanese
• Assessment of the cytocompatibility of ceramic nanoparticles with several types of cells
  Y. Era; S. Abe; Y. Nakagawa; A. Hyono; M. Nakamura; T. Kusaka; Y. Yoshida
  AsiaNANO 2016
  10 Oct. 2016 - 13 Oct. 2016
• リン酸エッチングの有無がユニバーサルアドヒーシブの象牙質接着耐久性に与える影響
  吉原久美子; 長岡紀幸; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 13 Oct. 2016, Japanese
• 接着性を有するMTA含有直接覆髄材の開発
  戸井田侑; 川野晋平; TING S; 長谷川智香; CHOWDHURY A; SAIKAEW P; ZUBAER A; 成徳英理; 成徳英理; 勝俣愛一郎; 福澤尚幸; 松本真理子; 角田晋一; 星加修平; 池田考績; 田中享; 島田康史; 田上順次; 網塚憲生; 吉田靖弘; 佐野英彦
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 13 Oct. 2016, Japanese
• バイオアクティブガラス含有歯質接着材の電子顕微鏡観察
  長岡紀幸; 吉原久美子; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 13 Oct. 2016, Japanese
• Bioactive glassフィラー含有コンポジットレジンの表面性状の検討
  吉原久美子; 長岡紀幸; 佐野英彦; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 01 Jun. 2016, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーの封鎖性に及ぼす根管洗浄剤の影響
  大嶌理紗; 菅谷勉; 下地伸司; 竹生寛恵; 川浪雅光; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 01 Jun. 2016, Japanese
• 従来型グラスアイオノマーセメントの電子顕微鏡観察
  長岡紀幸; 吉原久美子; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 01 Jun. 2016, Japanese
• 歯表面での3D微細構造構築によるバイオミメティック次世代歯科治療へ向けて
  赤坂司; 加我公行; 宮治裕史; 横山敦郎; 吉田靖弘
  日本歯科医学会誌, 31 Mar. 2016, Japanese
• Electronic states of alkyl radical functionalized fullerene (R-C60): Density Functional Theory (DFT) study
  S. Yoshizawa; S. Abe; T. Kusaka; M. Nakamura; Y. Yoshida; J. Iida; H. Tachikawa
  ISPlasma2016
  07 Mar. 2016 - 09 Mar. 2016
• 蛍光性セラミックスナノ粒子の細胞への適合性の評価
  木山望; 阿部薫明; 山方秀一; 吉田靖弘; 飯田順一郎
  日本矯正歯科学会大会プログラム・抄録集, 2016, Japanese
• 10‐MDP含有レジンセメントの象牙質接着界面の解析
  吉原久美子; 長岡紀幸; 吉田靖弘
  日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 01 Nov. 2015, Japanese
• Biomimetic catalytic macromolecules containing an active reaction center analogue of Carbonic Anhydrase: Appllication for carbonate ion induced reaction on apatite formation
  S. Yoshizawa; S. Abe; M. Yohda; M. Nakatani; A. Nishiyama-Chaya; Y. Fujii; A. Kusuhara; E. Yamatoya; T. Ishida; T. Akasaka; M. Nakamura; Y. Yoshida; J. Iida; T. Takada
  1st International Symposium of Institute for Catalysis - Global Collaboration in Catalysis Science toward Sustainable
  14 Oct. 2015 - 15 Oct. 2015
• 生体触媒安定化剤への利用に向けた高溶解性イオン液体中での酵素の長期安定性の検討
  金子恒太郎; 矢下亜紀良; 河合功治; 阿部薫明; 吉田靖弘
  日本生物工学会大会講演要旨集, 25 Sep. 2015, Japanese
• The development of long-term effectiveness silver ion-loaded antimicrobial agent
  吉原久美子; 長岡紀幸; 吉田靖弘
  日本歯科理工学会誌, 15 Sep. 2015, Japanese
• Cell alignment and migration of periodontal ligament fibroblasts on micro-patterns
  赤坂司; 加我公行; 横山敦郎; 阿部薫明; 吉田靖弘
  日本歯科理工学会誌, 15 Sep. 2015, Japanese
• 多糖複合体リン酸化プルランを用いた抗菌薬含有骨セメントの開発
  渡邉典行; 山根健太郎; 香川洋平; 篠原健介; 張偉; 吉田晶; 松川昭博; 吉田靖弘; 田中雅人; 尾崎敏文
  日本整形外科学会雑誌, 10 Sep. 2015, Japanese
• 新規生体材料によるインプラントコーティング
  香川洋平; 山根健太郎; 篠原健介; 渡邉典行; 張偉; 吉田晶; 吉田靖弘; 吉田靖弘; 尾崎敏文; 松川昭博
  日本整形外科学会雑誌, 10 Sep. 2015, Japanese
• Phosphorylated pullulan bioadhesive for regeneration and reconstruction of bone and tooth
  Yoshida Y; Okihara T; Nakamura M; Matsumoto T
  24th Symposium and Annual Meeting of the International Society for Ceramics in Medicine
  22 Oct. 2012 - 24 Oct. 2012
• 薬剤の濃度センサー機能を有したデリバリーシステム
  吉田靖弘; 難波尚子; 長岡紀幸; 中村真理子; 高柴正悟; 鈴木一臣
  第56回日本歯科理工学会学術講演会
  09 Oct. 2010 - 10 Oct. 2010
• Phosphorylated Pullulan Bioadhesive for Hard-Tissue Reconstruction
  Yoshida Y; Takahata, T; Nakamura M; Irie M; Tanaka M; Ozaki T; Suzuki K; Van Meerbeek, B
  88th General Session & Exhibition of the IADR
  14 Jul. 2010 - 17 Jul. 2010
• リン酸化多糖を担体とした抗菌物質デリバリーシステム
  吉田靖弘; 難波尚子; 長岡紀幸; 中村真理子; 高柴正悟; 鈴木一臣
  第54回日本歯科理工学会学術講演会
  01 Oct. 2009 - 02 Oct. 2009
• Chemical interaction of two functional monomers 4-MET and MDP to hydroxyapatite
  Yoshida Y; Yoshihara K; Nagaoka N; Hayakawa S; Osaka A; Minagi S; Suzuki K; Van Meerbeek B
  The Third International Congress on Adhesive Dentistry
  26 Sep. 2008 - 28 Sep. 2008
• Time-dependent molecular interaction at the monomer-hydroxy apatite interface
  Yoshida Y; Nagaoka N; Hayakawa S; Osaka A; Suzuki K; Van Meerbeek B
  IADR 86th General Session
  26 Sep. 2008 - 28 Sep. 2008
• Effect of CH-treatment of titanium on bone formation around implants
  Maekawa Kenji; Kawanishi Koji; Hayakawa Satoshi; Oshima Masamitsu; Shimono Kengo; Yoshida Yasuhiro; Suzuki Kazuomi; Osaka Akiyoshi; Kuboki Takuo
  IADR 86th General Session, 05 Jul. 2008
• Tissue-Biomaterial Interface Research for Prosthodontic Dentistry
  Yoshida, Y
  The International Symposium of the 117th Scientific Meeting of Japan Prosthodontic Society, The 1st Biennial Joint Congress of Chinese Prosthodontic Society, Invited oral presentation
  06 Jun. 2008 - 08 Jun. 2008
• Present Status and Future of Self-etching Systems
  Yoshida, Y
  International Symposium for Adhesive Dentistry 2008 in Kanazawa, Invited oral presentation
  16 Feb. 2008 - 17 Feb. 2008
• 機能性モノマーによるＣＰＣの固定化とその殺菌性
  第51回日本歯科理工学会学術講演会, 2008
• Modification of Ti surface with phosphorylated pullulan and photoreactive gelatin
  Yoshida, Y; Tanaka, T; Kuboki, T; Suzuki, K; Ito, Y
  International Dental Materials Congress 2007
  21 Nov. 2007 - 24 Nov. 2007
• Surface and interface control for hard tissues reconstruction
  Yoshida, Y
  The lecture at National Taiwan University, 19 Nov. 2007, Invited oral presentation
• Nano-interfacial control for hard tissue reconstruction
  Yoshida, Y
  The Annual Scientific Meeting of the Association for Dental Sciences of the Republic of China, Invited oral presentation
  16 Nov. 2007 - 18 Nov. 2007
• Surface and interface control for functional reconstruction of human hard tissues
  Yoshida, Y
  The lecture at the Leuven BIOMAT Research Cluster of the Catholic University of Leuven, 29 Jun. 2007
• Surface modification of Ti implants for promotion of bone regeneration.
  Yoshida Y; Suzuki K
  The 6th International Symposium on Titanium in Dentistry and The 2nd International Meeting on Titanium in Dental Technology
  05 Jun. 2007 - 06 Jun. 2007
• Nano-interfacial control for functional reconstruction of human hard tissues
  Yoshida, Y
  The 9th International Symposium on Biomimetic Interfaces, 01 Feb. 2007, Invited oral presentation
• 生体活性型口腔インプラントを目指したリン酸化糖処理チタンの検討
  第49回日本歯科理工学会学術大会, 2007
• Polyphosphoric Acid Treatment Promotes Bone Regeneration around Titanium Imprants
  85th General Session & Exhibition of the IADR, 2007
• Nano-interfacial control for functional reconstruction of human hard tissues.
  The 9th International Symposium on Biomimetic Interfaces, 2007
• Interaction of Phosphoric Acid Ester Monomer with Hydroxyapatite
  Yoshida, Y; Fukegawa, D; Hayakawa, S; Suzuki, K; Osaka, A; Van Meerbeek, B
  84th General Session & Exhibition of the IADR
  28 Jun. 2006 - 01 Jul. 2006
• 光反応性ゼラチンによるチタン表面の生物学的改善
  第48回日本歯科理工学会学術講演会, 2006
• シンポジウム「10 年後の歯科医療への提言―歯科理工学と関連領域とのコラボレーションー①」
  吉田 靖弘
  平成18 年度日本歯科理工学会近畿・中四国支部夏期セミナー, 2006, Nominated symposium
• Effect of HCl surface pre-treatment on resin bonding to titanium
  The IADR General Session & Exhibition, 2006
• Detection of Chemical Bonding at the Adhesive Biomaterial-Hydroxyapatite Interface
  The IADR General Session & Exhibition, 2006
• Ti-surface modification using polyphosphoric-acid enhanced osteoblast proliferation and bFGF adsorption
  The ADEA/AADR/CADR Meeting & Exhibition, 2006
• チタンーリン酸化アミノ酸誘導体界面反応のナノレベル解析
  第47回日本歯科理工学会学術講演会, 2006
• チタンへの接着性に及ぼすリン酸エステル系モノマーの影響
  第47回日本歯科理工学会学術講演会, 2006
• ラット創傷治癒モデルを用いた新規吸収性アパタイトの骨再生誘導の効果
  第53回国際歯科研究学会日本部会（JADR）総会・学術大会, 2005
• セルフエッチングシステムの接着安定性におよぼす機能性モノマーの影響
  第53回国際歯科研究学会日本部会（JADR）総会・学術大会, 2005
• Effect of functional monomers on bond durability of self-etch adhesives
  53th annual meeting of Japanese Association for Dental Research, 2005
• チタン表面と生体分子の相互作用解析のためのバイオセンサーの開発
  第25回日本口腔インプラント学会中国・四国支部学術大会総会, 2005
• Acceleration of cell behavior and growth factor adsorption onto titanium implant surface by polyphosphoric acid treatment
  The 1st Congress of Asian Academy of Osseointegration, 2005
• Initial Cell Attachment, Proliferation and Differentiation of Osteoblast Like (MC3T3-E1) Cells to Titanium and Chrome
  The 1st Congress of Asian Academy of Osseointegration, 2005
• Acceleration of cell behavior and growth factor adsorption onto titanium implant surface by polyphosphoric acid tretment
  社団法人 日本補綴歯科学会第114回学術大会, 2005
• チタンの接着耐久性を向上する新しい被着面処理法
  社団法人日本補綴歯科学会第114回学術大会, 2005
• 接着歯学と再生歯学のハーモナイゼイション
  第１回口腔顔面頭蓋再生研究国際シンポジウム, 2005
• 骨芽細胞様細胞株（MC3T3E1細胞）の細胞接着、増殖、分化および遺伝子発現に対するチタンの影響
  第35回社団法人日本口腔インプラント学会総会・学術大会, 2005
• 表面プラズモン解析装置を用いたチタンと組織接着性因子とのリアルタイム相互解析
  第46回日本歯科理工学会学術講演会, 2005
• ヒドロキシアパタイトとリン酸エステル系機能性モノマーの化学的相互作用
  第46回日本歯科理工学会学術講演会, 2005
• Titanium surface modification by polyphosphoric acid accelerated cell behavior and bFGF adsorption
  The 4th Biennial Congress of Asian Academy of Prosthodontics, 2005
• チタン表面への生体分子固体化
  第34回医用高分子シンポジウム, 2005
• 水酸化カルシウムあるいはレジン系接着システムによる直接覆髄が臓器に及ぼす影響
  日本歯科保存学会2005年度春季学会（第122回）, 2005
• Current bonding technology: can adhesive be easy-to-use and effective?
  The 2nd International Congress on Adhesive Dentistry, 2005
• Hydorolytic stability of 3 two-step self-etch adhesives bonded to dentin.
  Second International Congress on Adhesive Dentistry, 2005
• 機能性モノマーの違いがセルフエッチングシステムの象牙質長期接着安定性におよぼす影響
  第45回日本歯科理工学会学術講演会, 2005
• ポリリン酸処理によるチタンインプラントへの細胞応答促進と成長因子吸着制御
  第45回日本歯科理工学会学術講演会, 2005
• hBMSC attachment was enhanced by CTGF adsorption in vitro
  83st General Session of the IADR, 2005
• Acceleration of hMSC Attachment by Polyphosphoric Acid Adsorption onto Titanium
  83st General Session of the IADR, 2005
• Polyphosphoric acid adsorption onto Ti surface enhanced initial hMSC attachment
  83rd General Session & Exhibition of IADR, 2005
• Adsorption of polyphosphoric acid to titanium surface and its effect on hBMSC attachment
  International Symposium for Interface Oral Health Science, 2005
• Er:YAGレーザー照射エナメル質被着体の性質と接着
  第44回日本歯科理工学会学術講演会, 2004
• CTGF coating enhanced human bone marrow stromal cell attachment in vivo
  COAST, 2004
• Polyphosphoic acid adsorption onto Ti surface enhanced initial hMSC attachment
  COAST, 2004
• 歯科材料における接着界面の研究
  高分子学会高分子表面研究会, 2004
• トータルエッチングシステムによる樹脂含浸象牙質層の違いが長期接着耐久性に及ぼす影響
  第43回日本歯科理工学会学術講演会, 2004
• 表面プラズモン共鳴解析装置のためのチタンバイオセンサ-の開発
  第43回日本歯科理工学会学術講演会, 2004
• Effect of aging on the bonding effectiveness of six adhesives to class-I cavity bottom dentin
  Fifth International Symposium of Adhesive Dentistry, 2004
• Micro-tensile bond strength of a two-step self-etch adhesive to enamel/dentin with and with out prior phosphoric acid etching
  Fifth International Symposium of Adhesive Dentistry, 2004
• Micro-tensile bond strength of three two-step self-etch adhesives to dentin after long-term thermo-cycling
  Fifth International Symposium of Adhesive Dentistry, 2004
• Initial Cell Attachment and Proliferation of Osteoblasts on Titanium Surface
  82st General Session of the IADR, 2004
• Growth Factor Coating Enhanced Human Pulp-derived Cell Attachment in vitro
  82st General Session of the IADR, 2004
• Blood application followed by complete wash increased resin-dentin bonding
  IADR 82nd General Session, 2004
• Blood applicatin followed by complete wash increased resin-dentin bonding
  82st General Session of the IADR, 2004
• Bonding effectiveness of one-step self-etch adhesives to enamel and dentin
  82st General Session of the IADR, 2004
• Comparative Study on Adhesive Performance of Functional Monomers
  Yoshida, Y; Van Meerbeek, B; Okazaki, M; Shintani, H; Suzuki, K
  81th General Session & Exhibition of the IADR
  25 Jun. 2003 - 28 Jun. 2003
• Current status of dental adhesive technology
  8th Japan International SAMPE Symposium & Exhibition, 2003
• Osteoblast adhesion behavior onto functionally graded MgCO3 apatite.
  3rd Asian BioCeramics Symposium, 2003
• Chemical bonding potential of polyalkenoic acids to enamel and dentin.
  3rd Asian BioCeramics Symposium, 2003
• Filler Decontamination Methods Improving Degradation of Resin Composites.
  3rd Asian BioCeramics Symposium, 2003
• Er:YAGレーザー照射象牙質の脆弱層（レーザースメア層）と接着強さ
  第110回日本補綴歯科学会学術大会, 2003
• 自己硬化型水酸化カルシウム製剤の開発 第三報 自己硬化型水酸化カルシウム製剤が歯髄に及ぼす影響
  第42回日本歯科理工学会学術講演会, 2003
• チタンの接着に適した表面改質法の検討
  第42回日本歯科理工学会学術講演会, 2003
• Fabrication and Phase-composition Analysis of a Macroporous Biodegradable Ceramic
  81st General Session of the IADR, 2003
• Osteoblast Invasion into CO3Ap-Collagen Sponges
  81st General Session of the IADR, 2003
• Chemical Bonding Potential of Adhesive Materials to Hydroxyapatite
  81st General Session of the IADR, 2003
• １級窩洞に応用した各種象牙質接着システムの長期耐久性について
  日本歯科保存学会2003年度春季学会（118回）, 2003
• チタン製補綴物の接着特性に及ぼす表面処理の影響
  第109回日本補綴歯科学会学術大会, 2003
• 次世代の硬組織接着材料と技術
  第41回日本歯科理工学会学術講演会, 2003
• 炭酸アパタイト・コラーゲン複合体への骨芽細胞の侵入
  第41回日本歯科理工学会学術講演会, 2003
• 酸処理象牙質の収縮動態について（第２報）接触および非接触型プローブによる測定
  第42回日本歯科理工学会学術講演会, 2003
• Improved degradation resistance of composites containing pre-silanization decontaminated filler
  Forth International Congress on Dental Materials, 2002
• Optomized fller-matrix coupling for improved degradation resistance of resin composites
  The FDI Annual World Dental Congress 2002, 2002
• Micro-tensile bond strength of glass-ionomer adhesive to dentin after 4-year water storage
  PAN EUROPEAN FEDERATION 1st Annual Meeting, 2002
• 硬組織再生用アパタイト・コラーゲン複合体スポンジの細胞学的検討
  第40回日本歯科理工学会学術講演会, 2002
• リン酸処理時間が象牙質の脱灰コラーゲン層収縮および膨潤に及ぼす影響
  第40回日本歯科理工学会学術講演会, 2002
• 各種機能性モノマーがセルフエッチングシステムの象牙質接着性に及ぼす影響
  第40回日本歯科理工学会学術講演会, 2002
• 表面・界面分析の生体機能再生・再建学への応用
  平成14年度 日本歯科理工学会 近畿・中四国支部 夏期セミナー, 2002
• Improved degradation resistance of resin composites through optomized fller-matrix coupling
  First International Congress on Adhesive Dentistry, 2002
• 象牙質接着システム－crack形成とporosityの影響
  第39回日本歯科理工学会学術講演会, 2002
• 高気孔率を有するアパタイト・コラーゲン複合体の試作
  第39回日本歯科理工学会学術講演会, 2002
• Micro-tensile bond strength of 4 total-etch adhesives to dentin after 4-year water storage
  2002 IADR General Seccion, 2002
• フッ素化アパタイトの傾斜性概念
  第17回日本アパタイト研究会, 2001
• 骨芽細胞の接着特性並びに石灰化能に及ぼすMg2+の影響
  第21回日本マグネシウム研究会, 2001
• フィラー／マトリックス界面における結合強化－第1報 フィラー表面洗浄の効果－
  日本歯科保存学会2001年度秋季学会（第115回）, 2001
• 象牙質接着界面の形態学的および化学的研究－各種セルフエッチングシステムについて－
  日本歯科保存学会2001年度秋季学会（第115回）, 2001
• Adhesion affinity between osteoblast and MgCO3apatite-collagen composites
  Composites at Lake Louise 2001, 2001
• Mg含有炭酸アパタイト・コラーゲン複合体への骨芽細胞の接着特性
  第23回日本バイオマテリアル学会, 2001
• Resin-Modified Glass-Ionomer Cementsの象牙質接着界面における形態学的ならびに化学的検討
  第38回日本歯科理工学会学術講演会, 2001
• 象牙質接着システム―porosity分布とその影響 (接着理論解析の試み)―
  第38回日本歯科理工学会学術講演会, 2001
• 高強度コンポジットレジンのためのフィラー表面処理法について
  日本歯科理工学会 近畿・中四国支部夏期セミナー, 2001
• 歯質・修復物接着界面の形態学的および化学的検討
  日本歯科理工学会 近畿・中四国支部夏期セミナー, 2001
• Improved filler-matrix coupling in composites through pre-silanization decontamination
  79th General Session of the International Association for Dental Research, 2001
• Adhesion/Decalcification mechanisms of acid interactions with human hard tissues
  79th General Session of the International Association for Dental Research, 2001
• Interfacial characterization of resin-modified glass-ionomers bonded to dentin
  79th General Session of the International Association for Dental Research, 2001
• Analysis of chemical bonding potential of poly-acids to enamel and dentin
  79th General Session of the International Association for Dental Research, 2001
• Adhesion activity of osteoblast onto MgCO３Ap-collagen composites
  79th General Session of the International Association for Dental Research, 2001
• Chemical interaction of acids for titanium implant decontamination
  79th General Session of the International Association for Dental Research, 2001
• Micro-tensile bond strength of eleven modern adhesive systems to enamel
  79th General Session of the International Association for Dental Research, 2001
• XPSを用いたフィラー/シランカップリング剤の界面化学分析
  第37回日本歯科理工学会学術講演会, 2001
• 象牙質接着システム－collagen fibreの応力解析(脱灰象牙質のmicro-tensile bond test モデル)－
  第37回日本歯科理工学会学術講演会, 2001
• 重合開始剤の毒性およびエストロゲン活性についての検討
  第37回日本歯科理工学会学術講演会, 2001
• ポリカルボン酸の生体硬組織接着メカニズム
  未来開拓学術研究推進事業・第３回生体組織工学シンポジウム, 2001
• A new concept of CO３apatite-collagen composites with adhesion motif
  The 48th Annual Meeting of Japanese Association for Dental Research, 2000
• 象牙質接着システムー脱灰および健全象牙質の応力解析と諸因子の影響－micro-tensile bond testモデル－
  第36回日本歯科理工学会学術講演会, 2000
• Mg含有アパタイト・コラーゲン複合体の生体親和性に関する組織学的研究
  第36回日本歯科理工学会学術講演会, 2000
• 新機能性Mg2+含有炭酸アパタイト・コラーゲン複合体の創製
  日本バイオマテリアル学会シンポジウム2000, 2000
• 光重合レジンの溶出に関する研究（第３報）ラジカル発生と溶出CQの関係について
  第35回日本歯科理工学会学術講演会, 2000
• Mg含有アパタイト・コラーゲン複合体の表面性状と細胞接着特性に関する研究
  第35回日本歯科理工学会学術講演会, 2000
• コンポジットレジンの物性向上の為のフィラー表面洗浄法の確立
  第35回日本歯科理工学会学術講演会, 2000
• 象牙質接着システム－脱灰象牙質における応力解析モデル(micro-tensile bond test評価)－
  第35回日本歯科理工学会学術講演会, 2000
• Micro-tensile bond strength of an experimental resin-modified glass-ionomer adhesive
  78th General Session of the IADR, 2000
• TEM evaluation of self-etching adhesives’ resin-dentin interfaces
  78th General Session of the IADR, 2000
• Bonding mechanism and micro-tensile bond strength of a 4-MET-based self-etching adhesive
  78th General Session of the IADR, 2000
• Mechanism of adhesion/decalcification effects on human hard tissues by acids
  78th General Session of the IADR, 2000
• 傾斜機能性Mg含有炭酸アパタイト・コラーゲン複合体の細胞並びに組織との親和性
  第20回日本マグネシウム研究会, 2000
• 傾斜機能性Mg含有アパタイト・コラーゲン複合体の細胞接着特性
  未来開拓学術研究推進事業・第２回生体組織工学シンポジウム, 2000