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Sato Masanao
| Research Faculty of Agriculture Fundamental AgriScience Research Applied Bioscience | Associate Professor |
| Education and Research Center for Mathematical and Data Science | Associate Professor |
Researcher basic information
■ Degree■ URL
researchmap URLホームページURL■ Various IDs
Researcher number
- 20517693
Research KeywordResearch Field
- Life Science, Virology, Baculoviruses, Potyviruses
- Life Science, Genome biology, Gene regulatory networks, Transcriptome
- Bachelor's degree program, School of Agriculture
- Master's degree program, Graduate School of Agriculture
- Doctoral (PhD) degree program, Graduate School of Agriculture
Career
■ CareerCareer
- Jul. 2022 - Present
Hokkaido University, Graduate School of Agriculture Research Faculty of Agriculture Division of Applied Bioscience, Associate Professor, Japan - Apr. 2016 - Jun. 2022
Hokkaido University, Graduate School of Agriculture Research Faculty of Agriculture Division of Applied Bioscience, Assistant Professor, Japan - Apr. 2015 - Mar. 2016
Keio University, Institute for Advanced Biosciences, Project Assistant Professor, Japan - Aug. 2009 - Mar. 2015
National Institute for Basic Biology, Developmental Genetics, Assistant professor, Japan
Research activity information
■ Papers- Lobe‐Less, a Long Noncoding RNA That Regulates Drosophila Mushroom Body Morphogenesis
Sachi Inagaki; Natsuki Nakamura; Mitsutaka Kadota; Sean D. Keeley; Shigehiro Kuraku; Masanao Sato; Shinichi Nakagawa; Mari Mito; Kevin G. Nyberg; Kaori Niimi; Hidenori Kiyosawa; Satoru Kobayashi; Yuji Kageyama
Genes to Cells, Jan. 2026
Scientific journal - Amyloid-β-induced alteration of fast and localized calcium elevations in cultured astrocytes
Kaito Nakata; Joe Sakamoto; Kohei Otomo; Masanao Sato; Hirokazu Ishii; Motosuke Tsutsumi; Ryosuke Enoki; Tomomi Nemoto
Scientific Reports, 15, 1, Springer Science and Business Media LLC, 29 May 2025, [Peer-reviewed]
English, Scientific journal - Clinically approved chemical-controlled suppression of protein expression in BmN cells
Toshiki Nakanishi; Shin-ichiro Asano; Hisanori Bando; Masanao Sato
Journal of Insect Biotechnology and Sericology, 93, 1, 1, 10, 28 May 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal - The transcription regulator ChpA affects the global transcriptome including quorum sensing-dependent genes in Ralstonia pseudosolanacearum strain OE1-1.
Chika Takemura; Wakana Senuma; Masayuki Tsuzuki; Yuki Terazawa; Kanako Inoue; Masanao Sato; Akinori Kiba; Kouhei Ohnishi; Kenji Kai; Yasufumi Hikichi
Molecular plant pathology, 14 Jul. 2023, [International Magazine]
English, Scientific journal, The gram-negative plant-pathogenic β-proteobacterium Ralstonia pseudosolanacearum strain OE1-1 produces methyl 3-hydroxymyristate as a quorum sensing (QS) signal through methyltransferase PhcB and senses the chemical via the sensor histidine kinase PhcS. This leads to activation of the LysR family transcription regulator PhcA, which regulates the genes (QS-dependent genes) responsible for QS-dependent phenotypes, including virulence. The transcription regulator ChpA, which possesses a response regulator receiver domain and also a hybrid sensor histidine kinase/response regulator phosphore-acceptor domain but lacks a DNA-binding domain, is reportedly involved in QS-dependent biofilm formation and virulence of R. pseudosolanacearum strain GMI1000. To explore the function of ChpA in QS of OE1-1, we generated a chpA-deletion mutant (ΔchpA) and revealed that the chpA deletion leads to significantly altered QS-dependent phenotypes. Furthermore, ΔchpA exhibited a loss in its infectivity in xylem vessels of tomato plant roots, losing virulence on tomato plants, similar to the phcA-deletion mutant (ΔphcA). Transcriptome analysis showed that the transcript levels of phcB, phcQ, phcR, and phcA in ΔchpA were comparable to those in OE1-1. However, the transcript levels of 89.9% and 88.9% of positively and negatively QS-dependent genes, respectively, were significantly altered in ΔchpA compared with OE1-1. Furthermore, the transcript levels of these genes in ΔchpA were positively correlated with those in ΔphcA. Together, our results suggest that ChpA is involved in the regulation of these QS-dependent genes, thereby contributing to the behaviour in host plant roots and virulence of OE1-1. - The dataset of de novo assembly and inferred functional annotation of the transcriptome of Heterosigma akashiwo, a bloom-forming, cosmopolitan raphidophyte
Masanao Sato; Masahide Seki; Yutaka Suzuki; Shoko Ueki
Data in Brief, 48, 109071, 109071, Elsevier BV, Jun. 2023, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Construction of gene co-expression networks in cultured silkworm cells and identification of previously uncharacterized lepidopteran-specific genes required for chromosome dynamics.
Hiroaki Mon; Masanao Sato; Jae Man Lee; Takahiro Kusakabe
Insect biochemistry and molecular biology, 151, 103875, 103875, Dec. 2022, [International Magazine]
English, Scientific journal, Advances in sequencing technology and bioinformatics have accelerated gene discovery and homology-based functional annotation in many species, and numerous targeted gene studies have greatly expanded the understanding of gene functions. Nevertheless, there are still many genes that lack homology with genes in other evolutionary lineages and are left as genes with unknown functions. We constructed a gene co-expression network from the Bombyx mori ovary-derived cell line, BmN4, and attempted to infer the biological roles of uncharacterized genes based on the correlation between the function-known and unknown genes. Within this network, we focused on the co-expression modules involved in chromosome architecture, dynamics, and integrity, and selected the uncharacterized genes for subsequent RNAi-based phenotypic screening. This approach enabled the identification of 5 genes whose knockdown led to abnormalities in chromosome dynamics and spindle morphology in mitosis. One of them was a recently characterized gene, BmCenp-T, which plays a central role in building the kinetochore protein complex on the silkworm holocentric chromosomes. In this study, we suggest a method for constructing the gene co-expression network and selecting candidate genes for small-scale RNAi screening. This approach is complementary to homology-based annotation and may be useful for the analysis of lineage-specific uncharacterized genes such as orphan genes. - Intraspecies variation of the mitochondrial genome: An evaluation for phylogenetic approaches based on the conventional choices of genes and segments on mitogenome.
Jesús Morón-López; Karen Vergara; Masanao Sato; Gonzalo Gajardo; Shoko Ueki
PloS one, 17, 8, e0273330, 2022, [International Magazine]
English, Scientific journal, Intraspecies nucleotide sequence variation is a key to understanding the evolutionary history of a species, such as the geographic distribution and population structure. To date, numerous phylogenetic and population genetics studies have been conducted based on the sequences of a gene or an intergenic region on the mitochondrial genome (mtDNA), such as cytochrome c oxidase subunits or the D-loop. To evaluate the credibility of the usage of such 'classic' markers, we compared the phylogenetic inferences based on the analyses of the partial and entire mtDNA sequences. Importantly, the phylogenetic reconstruction based on the short marker sequences did not necessarily reproduce the tree topologies based on the analyses of the entire mtDNA. In addition, analyses on the datasets of various organisms revealed that the analyses based on the classic markers yielded phylogenetic trees with poor confidence in all tested cases compared to the results based on full-length mtDNA. These results demonstrated that phylogenetic analyses based on complete mtDNA sequences yield more insightful results compared to those based on mitochondrial genes and segments. To ameliorate the shortcomings of the classic markers, we identified a segment of mtDNA that may be used as an 'approximate marker' to closely reproduce the phylogenetic inference obtained from the entire mtDNA in the case of mammalian species, which can be utilized to design amplicon-seq-based studies. Our study demonstrates the importance of the choice of mitochondrial markers for phylogenetic analyses and proposes a novel approach to choosing appropriate markers for mammalian mtDNA that reproduces the phylogenetic inferences obtained from full-length mtDNA. - PhcQ mainly contributes to the regulation of quorum sensing-dependent genes, in which PhcR is partially involved, in Ralstonia pseudosolanacearum strain OE1-1
Takemura, C.; Senuma, W.; Hayashi, K.; Minami, A.; Terazawa, Y.; Kaneoka, C.; Sakata, M.; Chen, M.; Zhang, Y.; Nobori, T.; Sato, M.; Kiba, A.; Ohnishi, K.; Tsuda, K.; Kai, K.; Hikichi, Y.
Molecular Plant Pathology, 22, 12, 1538, 1552, Wiley, Dec. 2021, [Peer-reviewed]
Scientific journal - Proliferation of Bombyx mori nucleopolyhedrovirus strain H4 in BmN cells is enhanced by exchange of the F gene sequence with type strain T3.
Mami Sakai; Satoshi Kakutani; Shin-Ichiro Asano; Masanao Sato; Hisanori Bando
Virus research, 291, 198195, 198195, 02 Jan. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, The Bombyx mori nucleopolyhedrovirus (BmNPV)-based baculoviral expression vector system is among the most efficient expression vector systems for eukaryotic proteins especially when used in combination with silkworms as a host. We newly isolated a novel BmNPV strain (BmNPV H4) in Hokkaido, Japan that outperforms the type strain T3 in terms of both proliferation and expression of polyhedrin protein in silkworm larvae; however, it proliferates poorly in the BmN cell line. We inferred the gene responsible for the differences in proliferation between viral strains by quantifying amino acid similarity distances in protein functional domains and identifying highly divergent alleles between the H4 and T3 strains. Among proteins that differ markedly in functional domain sequence between H4 and T3, we identified the F gene, which encodes the F protein, as a putative cause of proliferative differences between the two strains. Using recombinant viruses with the F protein-coding sequence exchanged between H4 and T3, we determined that the T3 F protein increases H4 proliferation in BmN while the H4 F protein does not improve T3 proliferation in silkworm larvae. Our results suggest that the BmNPV F protein can strongly affect viral proliferation in a genetic background-specific manner and may be an important target for manipulating the proliferation characteristics of BmNPV-based expression vectors. - A single amino acid replacement of tyrosine with histidine at position 172 in BmNPV T3 GP64 decreases viral proliferation in silkworm larvae despite increasing membrane fusion activity with bmn cells
Sekiguchi, M.; Ishikawa, S.; Asano, S.-I.; B; o, H.; Sato, M.
Journal of Insect Biotechnology and Sericology, 89, 3, 45, 53, Oct. 2020, [Peer-reviewed], [Corresponding author]
English, Scientific journal - Design and prototyping of a bombyx mori nucleopolyhedrovirus-based genome assembly from pcr-amplified dna fragments
Harada, N.; Ishibashi, D.; Asano, S.-I.; Sato, M.; B; o, H.
Journal of Insect Biotechnology and Sericology, 87, 3, 97, 108, Oct. 2018, [Peer-reviewed], [Corresponding author]
English, Scientific journal - A mutation in plutella xylostella ABCC2 causes resistance to bacillus thuringiensis Cry1Ac by interfering with its receptor function
Nakaishi, Y.; Sato, M.; B; o, H.; Asano, S.-I.
Journal of Insect Biotechnology and Sericology, 87, 2, 45, 51, 2018, [Peer-reviewed]
English - Transcripts immunoprecipitated with Sxl protein in primordial germ cells of Drosophila embryos
Ryoma Ota; Shumpei Morita; Masanao Sato; Shuji Shigenobu; Makoto Hayashi; Satoru Kobayashi
DEVELOPMENT GROWTH & DIFFERENTIATION, 59, 9, 713, 723, Dec. 2017, [Peer-reviewed]
English, Scientific journal - Identification and functional analysis of outer kinetochore genes in the holocentric insect Bombyx mori
Hiroaki Mon; Jae Man Lee; Masanao Sato; Takahiro Kusakabe
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 86, 1, 8, Jul. 2017, [Peer-reviewed]
English, Scientific journal - Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure
Makoto Suzuki; Masanao Sato; Hiroshi Koyama; Yusuke Hara; Kentaro Hayashi; Naoko Yasue; Hiromi Imamura; Toshihiko Fujimori; Takeharu Nagai; Robert E. Campbell; Naoto Ueno
DEVELOPMENT, 144, 7, 1307, 1316, Apr. 2017, [Peer-reviewed]
English, Scientific journal - Conserved role of Ovo in germline development in mouse and Drosophila
Makoto Hayashi; Yuko Shinozuka; Shuji Shigenobu; Masanao Sato; Michihiko Sugimoto; Seiji Ito; Kuniya Abe; Satoru Kobayashi
SCIENTIFIC REPORTS, 7, 40056, Jan. 2017, [Peer-reviewed]
English, Scientific journal - Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses
Yuka Hagiwara-Komoda; Sun Hee Choi; Masanao Sato; Go Atsumi; Junya Abe; Junya Fukuda; Mie N. Honjo; Atsushi J. Nagano; Keisuke Komoda; Kenji S. Nakahara; Ichiro Uyeda; Satoshi Naito
SCIENTIFIC REPORTS, 6, 21411, Feb. 2016, [Peer-reviewed]
English, Scientific journal - Generation of an infectious cDNA clone of Okushiri virus and its derivative capable of expressing an exogenous gene
Yudistira Wahyu Kurnia; Ryosuke Fujita; Masanao Sato; Haruhiko Isawa; Shin Ichiro Asano; Ngo Dinh Binh; Hisanori Bando
Journal of Insect Biotechnology and Sericology, 85, 2, 39, 47, 2016, [Peer-reviewed]
English, Scientific journal - The distribution pattern of alpha 2,3-and alpha 2,6-linked sialic acids affects host cell preference in Toxoplasma gondii
Minami Baba; Masanao Sato; Katsuya Kitoh; Yasuhiro Takashima
EXPERIMENTAL PARASITOLOGY, 155, 74, 81, Aug. 2015, [Peer-reviewed]
English, Scientific journal - Ionotropic Glutamate Receptors Mediate Inducible Defense in the Water Flea Daphnia pulex
Hitoshi Miyakawa; Masanao Sato; John K. Colbourne; Taisen Iguchi
PLOS ONE, 10, 3, e0121324, Mar. 2015, [Peer-reviewed]
English, Scientific journal - Tightly Regulated Expression of Autographa californica Multicapsid Nucleopolyhedrovirus Immediate Early Genes Emerges from Their Interactions and Possible Collective Behaviors
Chikako Ono; Masanao Sato; Hitomi Taka; Shin-ichiro Asano; Yoshiharu Matsuura; Hisanori Bando
PLOS ONE, 10, 3, e0119580, Mar. 2015, [Peer-reviewed]
English, Scientific journal - H3K36 Trimethylation-Mediated Epigenetic Regulation is Activated by Bam and Promotes Germ Cell Differentiation During Early Oogenesis in Drosophila
Masanori Mukai; Seiji Hira; Katsuhiro Nakamura; Shoichi Nakamura; Hiroshi Kimura; Masanao Sato; Satoru Kobayashi
BIOLOGY OPEN, 4, 2, 119, 124, Feb. 2015, [Peer-reviewed]
English, Scientific journal - Detection of plant viruses in natural environments by using RNA-Seq.
Atsushi J Nagano; Mie N Honjo; Motohiro Mihara; Masanao Sato M; Hiroshi Kudoh
Methods in molecular biology (Clifton, N.J.), 1236, 89, 98, 2015, [Peer-reviewed], [Invited], [International Magazine]
English, Scientific journal, Sequencing of RNA by next generation sequencers, RNA-Seq, is revolutionizing virus detection. In addition to the unbiased detection of various viruses from wild plants in natural environments, RNA-Seq also allows for the parallel collection of host plant transcriptome data. Host transcriptome data are highly valuable for studying the responses of hosts to viral infections, as well as viral host manipulation. When detecting viruses using RNA-Seq, it is critical to choose appropriate methods for the removal of rRNA from total RNA. Although viruses with polyadenylated genomes can be detected by RNA-Seq following mRNA purification using oligo-dT beads, viruses with non-polyadenylated genomes are not effectively detected. However, such viruses can be detected by RNA-Seq using the rRNA selective depression method. The high-throughput and cost-effective method of RNA-Seq library preparation which is described here allows us to detect a broad range of viruses in wild plants. - Enrichment of Spermatogonial Stem Cells Using Side Population in Teleost
Makoto Hayashi; Masanao Sato; Yasuhiko Nagasaka; Sakiko Sadaie; Satoru Kobayashi; Goro Yoshizaki
BIOLOGY OF REPRODUCTION, 91, 1, 23, Jul. 2014, [Peer-reviewed]
English, Scientific journal - An effective method for the inference of reduced S-system models of Genetic Networks
Shuhei Kimura; Masanao Sato; Mariko Okada-Hatakeyama
IPSJ Transactions on Bioinformatics, 7, 30, 38, Information Processing Society of Japan, 2014, [Peer-reviewed]
English, Scientific journal - Inference of Vohradsky's Models of Genetic Networks by Solving Two-Dimensional Function Optimization Problems
Shuhei Kimura; Masanao Sato; Mariko Okada-Hatakeyama
PLOS ONE, 8, 12, e83308, Dec. 2013, [Peer-reviewed]
English, Scientific journal - Complex genetic interactions among non-essential genes of BmNPV revealed by multiple gene knockout analysis
Hitomi Taka; Chikako Ono; Masanao Sato; Shin-Ichiro Asano; Hisanori Bando
Journal of Insect Biotechnology and Sericology, 82, 1, 25, 32, 05 Aug. 2013
English, Scientific journal - Development of an efficient parameter estimation method for the inference of Vohradský's neural network models of genetic networks
Shuhei Kimura; Masanao Sato; Mariko Okada-Hatakeyama
Proceedings of the International Joint Conference on Neural Networks, 2013, [Peer-reviewed]
English, International conference proceedings - Dual Regulation of Gene Expression Mediated by Extended MAPK Activation and Salicylic Acid Contributes to Robust Innate Immunity in Arabidopsis thaliana
Kenichi Tsuda; Akira Mine; Gerit Bethke; Daisuke Igarashi; Christopher J. Botanga; Yayoi Tsuda; Jane Glazebrook; Masanao Sato; Fumiaki Katagiri
PLoS Genetics, 9, 12, e1004015, 2013, [Peer-reviewed]
English, Scientific journal - CBP60g and SARD1 play partially redundant critical roles in salicylic acid signaling
Lin Wang; Kenichi Tsuda; William Truman; Masanao Sato; Le V. Nguyen; Fumiaki Katagiri; Jane Glazebrook
PLANT JOURNAL, 67, 6, 1029, 1041, Sep. 2011, [Peer-reviewed]
English, Scientific journal - A Putative RNA-Binding Protein Positively Regulates Salicylic Acid-Mediated Immunity in Arabidopsis
Yiping Qi; Kenichi Tsuda; Anna Joe; Masanao Sato; Le V. Nguyen; Jane Glazebrook; James R. Alfano; Jerry D. Cohen; Fumiaki Katagiri
MOLECULAR PLANT-MICROBE INTERACTIONS, 23, 12, 1573, 1583, Dec. 2010, [Peer-reviewed]
English, Scientific journal - Network Modeling Reveals Prevalent Negative Regulatory Relationships between Signaling Sectors in Arabidopsis Immune Signaling
Masanao Sato; Kenichi Tsuda; Lin Wang; John Coller; Yuichiro Watanabe; Jane Glazebrook; Fumiaki Katagiri
PLOS PATHOGENS, 6, 7, e1001011, Jul. 2010, [Peer-reviewed]
English, Scientific journal - Endosome-Associated CRT1 Functions Early in Resistance Gene-Mediated Defense Signaling in Arabidopsis and Tobacco
Hong-Gu Kang; Chang-Sik Oh; Masanao Sato; Fumiaki Katagiri; Jane Glazebrook; Hideki Takahashi; Pradeep Kachroo; Gregory B. Martin; Daniel F. Klessig
PLANT CELL, 22, 3, 918, 936, Mar. 2010, [Peer-reviewed]
English, Scientific journal - Network Properties of Robust Immunity in Plants
Kenichi Tsuda; Masanao Sato; Thomas Stoddard; Jane Glazebrook; Fumiaki Katagiri
PLOS GENETICS, 5, 12, e1000772, Dec. 2009, [Peer-reviewed]
English, Scientific journal - Arabidopsis CaM Binding Protein CBP60g Contributes to MAMP-Induced SA Accumulation and Is Involved in Disease Resistance against Pseudomonas syringae
Lin Wang; Kenichi Tsuda; Masanao Sato; Jerry D. Cohen; Fumiaki Katagiri; Jane Glazebrook
PLOS PATHOGENS, 5, 2, e1000301, Feb. 2009, [Peer-reviewed]
English, Scientific journal - The Genetic Network Controlling the Arabidopsis Transcriptional Response to Pseudomonas syringae pv. maculicola: Roles of Major Regulators and the Phytotoxin Coronatine
Lin Wang; Raka M. Mitra; Keegan D. Hasselmann; Masanao Sato; Lisa Lenarz-Wyatt; Jerry D. Cohen; Fumiaki Katagiri; Jane Glazebrook
MOLECULAR PLANT-MICROBE INTERACTIONS, 21, 11, 1408, 1420, Nov. 2008, [Peer-reviewed]
English, Scientific journal - Interplay between MAMP-triggered and SA-mediated defense responses
Kenichi Tsuda; Masanao Sato; Jane Glazebrook; Jerry D. Cohen; Fumiaki Katagiri
PLANT JOURNAL, 53, 5, 763, 775, Mar. 2008, [Peer-reviewed]
English, Scientific journal - Interplay between MAMP-triggered and SA-mediated defense responses (Plant Journal (2008) 53, (763-775))
Tsuda, K.; Sato, M.; Glazebrook, J.; Cohen, J.D.; Katagiri, F.
Plant Journal, 55, 6, 1061, 1061, 2008
Scientific journal - Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean
Ma. Leonora M. Yambao; Haruka Yagihashi; Hiroshi Sekiguchi; Toru Sekiguchi; Toru Sasaki; Masanao Sato; Go Atsumi; Yoko Tacahashi; Kenji S. Nakahara; Ichiro Uyeda
Archives of Virology, 153, 1, 105, 115, Jan. 2008, [Peer-reviewed]
English, Scientific journal - Natural variation in RPS2-mediated resistance among Arabidopsis accessions: Correlation between gene expression profiles and phenotypic responses
Remco M. P. Van Poecke; Masanao Sato; Lisa Lenarz-Wyatt; Sanford Weisberg; Fumiaki Katagiri
PLANT CELL, 19, 12, 4046, 4060, Dec. 2007, [Peer-reviewed]
English, Scientific journal - Two resistance modes to Clover yellow vein virus in pea characterized by a green fluorescent protein-tagged virus
Marcelo Andrade; Masanao Sato; Ichiro Uyeda
PHYTOPATHOLOGY, 97, 5, 544, 550, May 2007, [Peer-reviewed]
English, Scientific journal - Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea
Gerald Ravelo; Uiko Kagaya; Tsuyoshi Inukai; Masanao Sato; Ichiro Uyeda
Journal of General Plant Pathology, 73, 1, 59, 65, Feb. 2007, [Peer-reviewed]
English, Scientific journal - A high-performance, small-scale microarray for expression profiling of many samples in Arabidopsis-pathogen studies
Masanao Sato; Raka M. Mitra; John Coller; Dong Wang; Natalie W. Spivey; Julia Dewdney; Carine Denoux; Jane Glazebrook; Fumiaki Katagiri
PLANT JOURNAL, 49, 3, 565, 577, Feb. 2007, [Peer-reviewed], [Lead author]
English, Scientific journal - System control in plant defense implicated by expression profiles during pathogen infection
Sato, M.; Katagiri, F.
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 52, 6 Suppl, 2007
Scientific journal - The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain
C Morita-Yamamuro; T Tsutsui; M Sato; H Yoshioka; M Tamaoki; D Ogawa; H Matsuura; T Yoshihara; A Ikeda; Uyeda, I; J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 46, 6, 902, 912, Jun. 2005, [Peer-reviewed]
English, Scientific journal - Selective involvement of members of the eukaryotic initiation factor 4E family in the infection of Arabidopsis thaliana by potyviruses
Masanao Sato; Kenji Nakahara; Moyasu Yoshii; Masayuki Ishikawa; Ichiro Uyeda
FEBS LETTERS, 579, 5, 1167, 1171, Feb. 2005, [Peer-reviewed], [Lead author]
English, Scientific journal - Natural resistance to Clover yellow vein virus in beans controlled by a single recessive locus
Masanao Sato; Chikara Masuta; Ichiro Uyeda
MOLECULAR PLANT-MICROBE INTERACTIONS, 16, 11, 994, 1002, Nov. 2003, [Peer-reviewed], [Lead author]
English, Scientific journal - Positional effect of gene insertion on genetic stability of a clover yellow vein virus-based expression vector
Zhen Dong Wang; Shigenori Ueda; Ichiro Uyeda; Haruka Yagihashi; Hiroshi Sekiguchi; Yoko Tacahashi; Masanao Sato; Kenji Ohya; Chihiro Sugimoto; Takeshi Matsumura
Journal of General Plant Pathology, 69, 327, 334, Oct. 2003, [Peer-reviewed]
English, Scientific journal - Development of clover yellow vein virus as an efficient, stable gene-expression system for legume species
Chikara Masuta; Toshikazu Yamana; Yoko Tacahashi; Ichiro Uyeda; Masanao Sato; Shigenori Ueda; Takeshi Matsumura
PLANT JOURNAL, 23, 4, 539, 546, Aug. 2000, [Peer-reviewed]
English, Scientific journal
- カイコゲノム配列解析の現状と展望
佐藤昌直, 日本応用動物昆虫学会大会講演要旨, 67th, 2023 - Transplanting the Human TBX5 Regulatory Elements: In Silico Screening and Evaluation of Transcription Factors Independent of Bombyx mori Transcriptional Regulation
前田麻亜子; 坪田拓也; 門宏明; 増田亮津; 松田拓也; 浅野眞一郎; 伴戸久徳; 瀬筒秀樹; 瀬筒秀樹; 荒川和晴; 荒川和晴; 日下部宜宏; 佐藤昌直; 佐藤昌直, 日本分子生物学会年会プログラム・要旨集(Web), 46th, 2023 - カイコ精巣をモデルとした配偶子形成に関与するカオナシ遺伝子の解析
柿野耕平; 門宏明; 佐藤昌直; 藤井告; 日野真人; 李在萬; 藤田龍介; 日下部宜宏, 蚕糸・昆虫機能利用学術講演会・日本蚕糸学会大会講演要旨集, 92nd, 2022 - 組織透明化を利用したBmNPV感染観察の高感度化
佐藤 拓海; 浅野 眞一郎; 佐藤 昌直, 東北蚕糸・昆虫利用研究報告, 46, 17, 22, Dec. 2021, [Last author]
Japanese - 遺伝子間相互作用の定量によって浮き彫りになる遺伝子の役割 : バキュロウイルスimmediate early遺伝子群を例に (特集 定量生物学事始め)
佐藤 昌直; 小野 慎子; 伴戸 久徳, 蚕糸・昆虫バイオテック, 89, 1, 15, 23, Apr. 2020
日本蚕糸学会, Japanese - 機能性糖質によるラット盲腸内細菌叢の改変に基づいたデオキシコール酸生成菌と相互作用する腸内細菌種の同定
吹谷智; 佐竹実菜子; 佐藤昌直; 石塚敏; 小椋義俊; 林哲也; 横田篤, 日本乳酸菌学会誌, 30, 2, 103, 2019 - データベースを利用したカイコのもつ遺伝子の相同性検索と分子系統樹の作成
横井翔; 天竺桂弘子; 天竺桂弘子; 佐藤昌直, 生物の科学 遺伝 (生物の科学 遺伝 別冊), 別冊No.23, 122, 128, 2019, [Invited]
Japanese, Introduction scientific journal - BmNPV必須遺伝子のリファクタリングとその評価
石川聡子; 佐藤昌直; 浅野慎一郎; 伴戸久徳, 東北蚕糸・昆虫機能利用研究報告, 43, 3, 6, Dec. 2018
Japanese - Bombyx mori nucleopolyhedrovirus immediate‐early遺伝子ORF上流500塩基による発現制御についての解析
工藤滉己; 佐藤昌直; 浅野眞一郎; 伴戸久徳, 東北蚕糸・昆虫利用研究報告, 42, 8‐11, 25 Dec. 2017
Japanese - マメコガネ幼虫におけるCry8Daトキシンの殺虫活性機構について
山口真; 佐藤昌直; 伴戸久徳; 浅野眞一郎, 東北蚕糸・昆虫利用研究報告, 42, 1‐3, 25 Dec. 2017
Japanese - バキュロウイルスの複数の非必須遺伝子がウイルス増殖に与える影響について
齋藤諒; 武田ひとみ; 佐藤昌直; 浅野眞一郎; 伴戸久徳, 東北蚕糸・昆虫利用研究報告, 42, 4‐7, 25 Dec. 2017
Japanese - Bridging -omics and reductionist approaches through signaling network modeling at system levels
Sato, M., Seikagaku, 88, 1, 54‐60, 25 Feb. 2016
Japanese - BmSyndecanノックダウンBmN細胞におけるBmNPVの増殖
宇多桃香; 佐藤昌直; 浅野眞一郎; 伴戸久徳, 東北蚕糸・昆虫利用研究報告, 40, 12, 14, 20 Dec. 2015
Japanese - 摂動に対するカイコ培養細胞株BmN4‐SID1のトランスクリプトーム変化とその情報を利用したネットワークモデリング
佐藤昌直; 佐藤昌直; 門宏明; 徐剣; 李在萬; 笠嶋めぐみ; 鈴木誉保; 坪田拓也; 小林悟; 瀬筒秀樹; 日下部宜宏, 日本生化学会大会(Web), 88th, 1P0798 (WEB ONLY), [1P0798], Dec. 2015
(公社)日本生化学会, Japanese - カイコにおけるTORシグナル経路の解明
平田一真; 佐藤昌直; 徐剣; 季明明; 祝力; 日野真人; 山下真実; 諸熊大輔; 門宏明; 李在萬; 日下部宜宏, 蚕糸・昆虫機能利用学術講演会・日本蚕糸学会大会講演要旨集, 85th, 2015 - バキュロウイルスゲノム人工合成系の確立に向けて
石橋大樹; 佐藤昌直; 高ひとみ; 武内潤一; 浅野眞一郎; 伴戸久徳, 東北蚕糸・昆虫利用研究報告, 39, 5, 7, 10 Dec. 2014
Japanese - Toward a systems understanding of plant-microbe interactions
Akira Mine; Masanao Sato; Kenichi Tsuda, FRONTIERS IN PLANT SCIENCE, 5, 423, Aug. 2014, [Peer-reviewed]
English, Book review - BmNPVの非必須遺伝子(Bm32‐Bm36)間相互作用解析
高ひとみ; 小野慎子; 佐藤昌直; 浅野眞一郎; 伴戸久徳, 東北蚕糸・昆虫利用研究報告, 38, 1, 3, 30 Dec. 2013
Japanese - Not to be suppressed? Rethinking the host response at a root-parasite interface
Derek B. Goto; Hikota Miyazawa; Jessica C. Mar; Masanao Sato, PLANT SCIENCE, 213, 9, 17, Dec. 2013, [Peer-reviewed], [Last author]
English, Book review - カイコ分散型動原体染色体ループドメイン形成へのCTCF,CP190の関与
益田篤; 佐藤昌直; 末次克行; 山本公子; 門宏明; 李在萬; 日下部宜宏, 蚕糸・昆虫機能利用学術講演会・日本蚕糸学会大会講演要旨集, 83rd, 2013 - BmDnmt1,BmDnmt2,bMBDの機能阻害細胞におけるDNAのメチル化
満留卓実; 門宏明; 佐藤昌直; 末次克行; 山本公子; 李志清; 徐剣; 祝力; 李在萬; 日下部宜宏, 蚕糸・昆虫機能利用学術講演会・日本蚕糸学会大会講演要旨集, 83rd, 2013 - Expression profiles as detailed snapshots of biological states
Fumiaki Katagiri; Masanao Sato, TRANSGENIC RESEARCH, 16, 4, 399, 403, Aug. 2007, [Peer-reviewed], [Invited], [Last author]
English, Others - 植物と微生物および昆虫との相互作用 遺伝子発現パターンから見た植物の防御応答システム
佐藤昌直; 片桐文章, 蛋白質 核酸 酵素, 52, 6, 654, 659, 10 May 2007
Japanese - Host factors and its relevance to virus infection in plants
SATO Masanao; WATANABE Yuichiro, ウイルス, 56, 2, 155, 164, 22 Dec. 2006, [Peer-reviewed]
Virus infection is established when viral proteins can interact with host factors to execute replication and/or cell-to-cell movement. Even after the virus infection has started, host resistance reactions, if trigged, would suppress further virus propagation. We would like to introduce what we understand about host factors as determinants of infection establishment and as key resistance molecules. Genome-wide information of Arabidopsis is providing us much information about such host factors involved in virus infection., The Japanese Society for Virology, Japanese
- カイコの実験単 : カイコで生命科学をまるごと理解!
「カイコの実験単」企画・編集委員会; 日本蚕糸学会; 金児, 雄; 塩見, 邦博; 天竺桂, 弘子; 外川, 徹; 横山, 岳(蚕糸学)
エヌ・ティー・エス, Mar. 2019, 9784860435981, 303p, Japanese, [Joint work]
- BmNPVにおける膜タンパク質(GP64)のアミノ酸バリアントが増殖に与える影響
関口真理; 佐藤昌直; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2020
2020 - 2020 - 蛍光レポーター発現細胞クラスター認識を自動化・定量化する画像解析アプローチ
中西登志紀; 関口真理; 浅野眞一郎; 伴戸久徳; 佐藤昌直
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2020
2020 - 2020 - Identification of key targets and components of PRC2-mediated systemic defense priming in Arabidopsis thaliana
田島由理; 佐藤昌直; LOO Eliza Po-Iian; 西條雄介
日本植物病理学会大会プログラム・講演要旨予稿集, 2020
2020 - 2020 - Polycomb repressive complex 2 positively regulates systemic immunity and priming in Arabidopsis thaliana
Yuri Tajima; Masanao Sato; Eva-Maria Reimer-Michalski; Eliza Po-iian Loo; Barbara Kracher; Franziska Turck; Yusuke Saijo
2019 IS-MPMI XVIII Congress, 15 Jul. 2019 - カイコに存在する2つのTOR遺伝子の機能的違い
小林政彦; 門宏明; 李在萬; 佐藤昌直; 藤田龍介; 日下部宜宏
日本分子生物学会年会プログラム・要旨集(Web), 2019
2019 - 2019 - ポリコーム抑制複合体を介した植物免疫プライミング機構
田島由理; REIMER-MICHALSKI Eva-Maria; LOO Eliza Po-Iian; KRACHER Barbara; TURCK Franziska; 佐藤昌直; 西條雄介; 西條雄介
日本植物病理学会大会プログラム・講演要旨予稿集, 2019
2019 - 2019 - BmN細胞におけるSmall Molecule Assisted Shutoffの検証
中西登志紀; 佐藤昌直; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2019
2019 - 2019 - カイコ核多角体病ウイルス新規株H4の膜タンパク質GP64の機能解析
関口真理; 佐藤昌直; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2019
2019 - 2019 - カイコRNA-seqデータ自動解析および可視化プログラムの作成と利用
佐藤昌直; 横井翔; 坪田拓也; 瀬筒秀樹
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2019
2019 - 2019 - 機能未知遺伝子ライブラリーから得られたカイコ分散型動原体を構成する新規遺伝子の解析
門宏明; 佐藤昌直; 藤田龍介; 李在萬; 日下部宜宏
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2019
2019 - 2019 - Gene ontology解析:トランスクリプトーム情報から生物学的解釈を得る
佐藤昌直
日本植物病理学会植物感染生理談話会論文集, 2019
2019 - 2019 - 大量ショートリードデータからトランスクリプトーム情報を抽出する
佐藤昌直
日本植物病理学会植物感染生理談話会論文集, 2019, Public discourse
2019 - 2019 - 機能性糖質によるラット盲腸内細菌叢の改変に基づいたデオキシコール酸生成菌と相互作用する腸内細菌種の同定
吹谷智; 佐竹実菜子; 佐藤昌直; 石塚敏; 小椋義俊; 林哲也; 横田篤
日本乳酸菌学会誌, 2019
2019 - 2019 - Lobe‐less RNAはショウジョウバエの胚発生期において軸索走行制御に寄与する長鎖ノンコーディングRNAである
稲垣幸; 門田満隆; KEELEY Sean D; KEELEY Sean D; 中村奈月; 佐藤昌直; 工樂樹洋; 影山裕二; 影山裕二
日本RNA学会年会要旨集, 09 Jul. 2018, Japanese - ネッタイシマカにおけるCry44Aaトキシンレセプターの評価
浅野眞一郎; 小野山雄亮; 中神あゆみ; 中尾悠太; 佐藤昌直; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 19 Mar. 2018, Japanese - in vitro DNA連結によるBmNPVゲノム構築プラットフォームの確立
原田直人; 石橋大樹; 浅野眞一郎; 佐藤昌直; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 19 Mar. 2018, Japanese - Pseudomonas syringae pv. tabaciのNicotiana tabacumに対する感染挙動
丸山望; 石賀貴子; 石賀康博; 佐藤昌直; 鈴木智子; 加来久敏; 尾花望; 一瀬勇規; 野村暢彦; 別役重之
日本植物病理学会大会プログラム・講演要旨予稿集, 12 Mar. 2018, Japanese - ポリコーム抑制複合体を介した植物免疫プライミングの分子制御機構の解明
田島由理; REIMER‐MICHALSKI Eva‐Maria; LOO Eliza Po‐iian; 白石菜月; KRACHER Barbara; TURCK Franziska; 佐藤昌直; 西條雄介; 西條雄介
日本植物病理学会大会プログラム・講演要旨予稿集, 12 Mar. 2018, Japanese - ラットへのコール酸および難消化性オリゴ糖の同時投与による腸内細菌叢改変に基づいたデオキシコール酸生成菌と相互作用する腸内細菌種の探索
佐竹実菜子; 吹谷智; 佐藤昌直; 石塚敏; 小椋義俊; 林哲也; 横田篤
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2018, Japanese - ウイルス感染時におけるAGO1-vsiRNAの標的となる植物mRNAの予測
堀裕和; 岩橋美穂; 白谷公孝; 佐藤昌直; 峯彰; 峯彰; 竹田篤史; 竹田篤史; 竹田篤史
日本分子生物学会年会プログラム・要旨集(Web), 2018
2018 - 2018 - Lobe-less RNAはショウジョウバエの胚発生期において軸索走行制御に寄与する長鎖ノンコーディングRNAである
稲垣幸; 門田満隆; KEELEY Sean D.; KEELEY Sean D.; 中村奈月; 佐藤昌直; 工樂樹洋; 影山裕二; 影山裕二
日本RNA学会年会要旨集, 2018
2018 - 2018 - ショウジョウバエLobe‐less RNAは胚発生期の軸索走行シグナルを制御している
稲垣幸; 中野美幸; 中村奈月; 門田満隆; KEELEY Sean D; 佐藤昌直; 工樂樹洋; 影山裕二; 影山裕二
日本RNA学会年会要旨集, 19 Jul. 2017, Japanese - 〔Major achievements〕「顔無し遺伝子」ハンティングパイプラインの構築:配列情報から機能を推定できない遺伝子の機能推定
佐藤昌直; 門宏明; 日下部宜宏
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 17 Mar. 2017, Japanese - ネッタイシマカにおけるCry44Aaトキシンレセプターについて
浅野眞一郎; 中神あゆみ; 中尾悠太; 佐藤昌直; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 17 Mar. 2017, Japanese - 〔Major achievements〕BmNPV非必須遺伝子arif‐1,pif‐2による補償的な必須遺伝子発現制御
高ひとみ; 佐藤昌直; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 17 Mar. 2017, Japanese - 動的なカルシウムシグナルによる頂端収縮と神経管閉鎖の制御
鈴木誠; 佐藤昌直; 小山宏史; 上野直人
日本数理生物学会年会(Web), 2017
2017 - 2017 - ショウジョウバエLobe-less RNAは胚発生期の軸索走行シグナルを制御している
稲垣幸; 中野美幸; 中村奈月; 門田満隆; KEELEY Sean D.; 佐藤昌直; 工樂樹洋; 影山裕二; 影山裕二
日本RNA学会年会要旨集, 2017
2017 - 2017 - BmNPV非必須遺伝子arif-1,pif-2による補償的な必須遺伝子発現制御
高ひとみ; 佐藤昌直; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2017
2017 - 2017 - 植物micro‐RNAの認識特異性に関する研究とその応用
白谷公孝; 舛田さくら; 長田怜子; 唐戸俊介; 都筑正行; 佐藤昌直; 渡邊雄一郎; 竹田篤史
日本植物学会大会研究発表記録, 01 Sep. 2016, Japanese - RNA‐seq法を用いた核多角体病ウイルス感染カイコ細胞のトランスクリプトーム解析
浜島りな; 佐藤昌直; 小林迪弘; 池田素子
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 16 Mar. 2016, Japanese - MAPKの基質となるWRKY型転写因子の葉緑体ROSバーストへの関与
安達広明; 波多江健太; 佐藤昌直; 吉岡博文
日本植物病理学会大会プログラム・講演要旨予稿集, 03 Mar. 2016, Japanese - 植物micro-RNAの認識特異性に関する研究とその応用
白谷公孝; 舛田さくら; 長田怜子; 唐戸俊介; 都筑正行; 佐藤昌直; 渡邊雄一郎; 竹田篤史
日本植物学会大会研究発表記録, 2016
2016 - 2016 - RNA-seq法を用いた核多角体病ウイルス感染カイコ細胞のトランスクリプトーム解析
浜島りな; 佐藤昌直; 小林迪弘; 池田素子
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2016
2016 - 2016 - カイコにおけるTORシグナル経路の解明
平田一真; 佐藤昌直; 徐剣; 季明明; 祝力; 日野真人; 山下真実; 諸熊大輔; 門宏明; 李在萬; 日下部宜宏
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 25 Sep. 2015, Japanese - カイコ卵巣由来培養細胞BmN‐BmNPVトランスクリプトームにおけるmRNA配列の推定と評価
佐藤昌直; 佐藤昌直; 高ひとみ; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 25 Sep. 2015, Japanese - BmSyndecanノックダウンBmN細胞におけるBmNPVの増殖
宇多桃香; 佐藤昌直; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 25 Sep. 2015, Japanese - 遺伝子ネットワークのreduced S-systemモデルの効率的同定法の提案
木村 周平; 佐藤 昌直; 岡田(畠山) 眞里子
情報処理学会研究報告. BIO, バイオ情報学, 13 Mar. 2015, 一般社団法人情報処理学会, Japanese
13 Mar. 2015 - 13 Mar. 2015, 遺伝子ネットワーク同定とは,観察された遺伝子発現データに合うような遺伝子ネットワークのモデルのパラメータを推定する問題のことである.遺伝子ネットワークを記述するために多くの数理モデルが提案されているが,中でも S-system モデルは最も使用されてモデルの一つである. S-system モデルは生化学反応を近似したモデルであり,遺伝子ネットワークの記述に適していると考えられている.そのため S-system モデルを用いた遺伝子ネットワーク同定法が,これまでに数多く提案されている.しかしながら S-system に含まれるパラメータ数は,他の頻繁に使用されているモデルのパラメータ数よりも多い.従って遺伝子ネットワークの S-system モデルを同定するためには,より多くの遺伝子発現データを与える必要があると考えられる.遺伝子ネットワーク同定に必要な遺伝子発現データの量を減らすために,本研究では S-system モデルの一部のパラメータを 0 に固定することでモデルを単純化する.本研究では単純化した S-system モデルを,reduced S-system モデルと呼ぶ.次に遺伝子ネットワークの reduced S-system モデルを効率的に同定するための新たな方法を提案する.最後に数値実験を通して,提案手法の有効性を示す. - クローバ葉脈黄化ウイルスがコードするP3N‐PIPOおよび新規タンパク質の発現機構
薦田(萩原)優香; CHOI S. H; 佐藤昌直; 厚見剛; 阿部純也; 中原健二; 上田一郎; 内藤哲
日本植物病理学会大会プログラム・講演要旨予稿集, 10 Mar. 2015, Japanese - バキュロウイルスゲノム人工合成系の確立
石橋大樹; 佐藤昌直; 高ひとみ; 武内潤一; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 2015
2015 - 2015 - 摂動に対するカイコ培養細胞株BmN4‐SID1のトランスクリプトーム変化とその情報を利用したネットワークモデリング
佐藤昌直; 佐藤昌直; 門宏明; 徐剣; 李在萬; 笠嶋めぐみ; 鈴木誉保; 坪田拓也; 小林悟; 瀬筒秀樹; 日下部宜宏
日本生化学会大会(Web), 2015, Japanese - 植物のmicroRNA‐標的mRNA間に1~3ケ所のミスマッチがある際の認識特異性に関する研究
白谷公孝; 舛田さくら; 長田怜子; 唐戸俊介; 都筑正行; 佐藤昌直; 佐藤昌直; 渡邊雄一郎; 竹田篤史; 竹田篤史
日本生化学会大会(Web), 2015, Japanese - Lobe‐less RNAはポリコーム複合体と相互作用し,ショウジョウバエにおけるキノコ体の形態形成を制御している
稲垣幸; 佐藤昌直; 中村奈月; 小林悟; 影山裕二
日本RNA学会年会要旨集, 23 Jul. 2014, Japanese - AcMNPV IE遺伝子発現のロバストネスを支えるIE遺伝子ネットワークと補償的相互作用
小野慎子; 高ひとみ; 佐藤昌直; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 09 Mar. 2014, Japanese - 非必須遺伝子の複数ノックアウトがBmNPV増殖に与える影響について
高ひとみ; 小野慎子; 佐藤昌直; 浅野眞一郎; 伴戸久徳
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 09 Mar. 2014, Japanese - カイコDNMT‐1(BmDNMT‐1)の生化学的特性と遺伝子転写制御における役割
満留卓実; 門宏明; 佐藤昌直; 末次克行; 山本公子; 李志清; 徐剣, 祝力; LEE Jae M; 日下部宜宏; 益田篤
日本分子生物学会年会プログラム・要旨集(Web), 2014, Japanese - BmDnmt1,BmDnmt2,bMBDの機能阻害細胞におけるDNAのメチル化
満留卓実; 門宏明; 佐藤昌直; 末次克行; 山本公子; 李志清; 徐剣, 祝力; 李在萬; 日下部宜宏
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 18 Mar. 2013, Japanese - カイコ分散型動原体染色体ループドメイン形成へのCTCF,CP190の関与
益田篤; 佐藤昌直; 末次克行; 山本公子; 門宏明; 李在萬; 日下部宜宏
日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集, 18 Mar. 2013, Japanese - Lobe‐less RNAはショウジョウバエキノコ体の形態形成を介して学習・記憶を制御している
稲垣幸; 佐藤昌直; 宮下知之; 中村奈月; 小林悟; 斉藤実; 影山裕二
日本分子生物学会年会プログラム・要旨集(Web), 2013, Japanese - Side populationを指標にしたニジマス精原幹細胞の濃縮
林誠; 佐藤昌直; 岩崎佳子; 小野澤健明; 長坂安彦; 定家咲子; 小林悟; 吉崎悟朗
日本分子生物学会年会プログラム・要旨集(Web), 2012, Japanese - カイコ分散型動原体染色体におけるクロマチンループ形成とその役割
益田篤; 佐藤昌直; 門宏明; LEE Jae Man; 日下部宜宏
日本分子生物学会年会プログラム・要旨集(Web), 2012, Japanese - サリチル酸,ジャスモン酸,エチレンのシグナル伝達を司る遺伝子の多重変異体の解析は,植物抵抗性メカニズムにおけるそれらの協調的影響を明らかにした
津田賢一; 佐藤昌直; STODDARD Thomas; GLAZEBROOK Jane; 片桐文章
日本植物病理学会報, 25 Aug. 2009, Japanese - mRNA発現プロファイリングおよびネットワーク解析を基盤とした機能ゲノミクス
佐藤昌直; LENARZ‐WYATT Lisa; HERNICK Charles; GLAZEBROOK Jane; 渡辺雄一郎; 片桐文章
日本植物生理学会年会要旨集, 16 Mar. 2009, Japanese - 植物ホルモンの情報伝達を司る遺伝子の多重変異が植物の病原体抵抗性に与える影響
津田賢一; 佐藤昌直; STODDARD Thomas; GLAZEBROOK Jane; 片桐文章
日本植物生理学会年会要旨集, 16 Mar. 2009, Japanese - (403) Mutual Inhibition between Salicylate-mediated and MAMPs-induced Signaling Revealed by a Network Analysis(Abstracts of the Papers Presented at the Annual Meeting of the Society)
Sato M.; Tsuda K.; Glazebrook Jane; Watanabe Y.; Katagiri F.
Annals of the Phytopathological Society of Japan, 25 Aug. 2008, The Phytopathological Society of Japan (PSJ), English
25 Aug. 2008 - 25 Aug. 2008 - MAMPによって誘導される防御応答におけるSAシグナリングの重要性
津田賢一; 佐藤昌直; GLAZEBROOK Jane; COHEN Jerry; 片桐文章
日本植物生理学会年会要旨集, 15 Mar. 2008, Japanese - シロイヌナズナの防御応答シグナル伝達のネットワークレベルでの解析
佐藤昌直; 津田賢一; GLAZEBROOK Jane; 渡辺雄一郎; 片桐文章
日本植物生理学会年会要旨集, 15 Mar. 2008, Japanese - (283) Statistical Models that Predict the in planta Bacterial Growth based on Early Expression Levels of a Small Number of Arabidopsis Genes in the RPS2-mediated Resistance(Abstracts of the Papers Presented at the Annual Meeting of the Society)
Sato M.; Lenarz-Wtatt Lisa; Watanabe Y.; Katagiri F.
Annals of the Phytopathological Society of Japan, 25 Aug. 2007, The Phytopathological Society of Japan (PSJ), English
25 Aug. 2007 - 25 Aug. 2007 - 遺伝子発現プロファイリングによる,シロイヌナズナのPseudomonas syringae hrcC変異体に対する防御応答解析
津田賢一; 佐藤昌直; 片桐文章
日本植物生理学会年会要旨集, 15 Mar. 2007, Japanese - シロイヌナズナのPseudomonas syringaeに対する防御応答を制御するシグナル伝達ネットワークの解析
佐藤昌直; LENARZ‐WYATT Lisa; 渡辺雄一郎; 片桐文章
日本植物生理学会年会要旨集, 15 Mar. 2007, Japanese - Analysis of the Arabidopsis thaliana Signaling Network Controlling Defense Responses Against the Bacterial Pathogen Pseudomonas syringae
Sato Masanao; Lenarz-Wyatt Lisa; Watanabe Yuichiro; Katagiri Fumiaki
Plant and Cell Physiology Supplement, 2007, The Japanese Society of Plant Physiologists, English
2007 - 2007, Elucidation of the plant defense signaling network controlling responses to pathogen infection, has been challenging for several biological reasons. First, plant defense signaling pathways are highly interconnected. Second, plants receive multiple stimuli associated with pathogen infection including pathogen-associated molecular patterns and effectors delivered directly into plant cells. Third, part of the signaling network is robust to perturbations.
To reveal the structure and function of this signaling network, we have employed a strategy using parallel and quantitative data collection. Our objective is to collect hundreds of observations about the behavior of the system after introducing specific perturbations. To this end, we have developed a high-performance, inexpensive custom microarray and have initiated gene expression profiling combined with a reverse genetics strategy. Analysis of known mutants as reference points within the signaling network revealed a model for the topology of the network and key players controlling signaling pathways utilized to process different stimuli. - Expression Profile Analysis of Arabidopsis Defense Responses Elicited by The Pseudomonas syringae hrcC Mutant
Tsuda Kenichi; Sato Masanao; Katagiri Fumiaki
Plant and Cell Physiology Supplement, 2007, The Japanese Society of Plant Physiologists, English
2007 - 2007, Plants change expression of numerous genes upon pathogens attack, and many of the changes compose plant defense mechanisms. The hrcC mutant of the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 is defect in injecting type III effectors (T3Es) into the plant cell, and plant responses induced by the mutant strain is considered mostly based on recognition of pathogen-associated molecular patterns (PAMPs). It is not clear whether PAMPs have any effects on salicylic acid (SA) -mediated responses, which are induced by recognition of some T3Es. Several Arabidopsis defense mutants were analyzed by mRNA profiling using a small-scale custom microarray after inoculation of the hrcC strain. We found that induction of some genes by the hrcC strain depends on the ICS1 gene, which is essential for the pathogen-induced SA synthesis. This strongly suggests that PAMPs can induce SA-mediated defense responses. We will also discuss expression profiles of other Arabidopsis mutants. - (212) Development of an Arabidopsis Pathoarray to Study Arabidopsis thaliana-pathogen Interactions(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)
Sato M.; Mitra Raka M.; Poecke Remco M. van; Collar John; Glazebrook Jane; Katagin F.
Annals of the Phytopathological Society of Japan, 25 Nov. 2006, The Phytopathological Society of Japan (PSJ), English
25 Nov. 2006 - 25 Nov. 2006 - シロイヌナズナ‐病原体相互作用大規模解析用マイクロアレイ“Arabidopsis Pathoarray”の開発
佐藤昌直; MITRA Raka M; VAN POECKE Remco M; COLLER John; GLAZEBROOK Jane; 片桐文章
日本植物病理学会報, 25 Nov. 2006, English - (347) Characterization of the Resistance in Pea to Clover Yellow Vein Virus using a Green Fluorescent Protein Tagged Virus(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)
Andrade M.; Sato M.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Aug. 2005, The Phytopathological Society of Japan (PSJ), English
25 Aug. 2005 - 25 Aug. 2005 - (293) Isolation of Resistance-breaking Strains of Clover yellow vein virus from Pea(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)
Andrade M.; Yambao M. L. M.; Sato M.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Aug. 2004, The Phytopathological Society of Japan (PSJ), English
25 Aug. 2004 - 25 Aug. 2004 - (7) Resistance to Clover yellow vein virus (C1YVV) in Some Lines of Pea is Governed by a Single Recessive Gene.(Abstracts Presented at the Meeting of the Hokkaido Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)
Andrade M.; Sato M.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Feb. 2004, The Phytopathological Society of Japan (PSJ), English
25 Feb. 2004 - 25 Feb. 2004 - (6)A Point Mutation in the Helper Component-protease (HC-Pro) Gene of Clover yellow vein virus Associates with Symptom Attenuation in Broad Beans.(Abstracts Presented at the Meeting of the Hokkaido Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)
Yambao M. L. M.; Sato M.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Feb. 2004, The Phytopathological Society of Japan (PSJ), English
25 Feb. 2004 - 25 Feb. 2004 - (5) Genetic Analyses of Symptom Determinants in Clover yellow vein virus-infected Pea (Abstracts Presented at the Meeting of the Hokkaido Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)
Kagaya U.; Sato M.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Feb. 2004, The Phytopathological Society of Japan (PSJ), Japanese
25 Feb. 2004 - 25 Feb. 2004 - (427)Fine Mappinf of the Pathogenic Determinants of Clover yellow vein virus Genome(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)
Yambo M. L. M.; Sasaki T.; Ueda I.; Sato M.; Sekiguchi H.; Yagihashi H.; Takahashi Y.
Annals of the Phytopathological Society of Japan, 25 Aug. 2003, The Phytopathological Society of Japan (PSJ), Japanese
25 Aug. 2003 - 25 Aug. 2003 - Comparison of Reactions to Clover yellow vein virus Infection between Different Pea Cultivars(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)
Sato M.; Ueda I.
Annals of the Phytopathological Society of Japan, 25 Aug. 2003, The Phytopathological Society of Japan (PSJ), Japanese
25 Aug. 2003 - 25 Aug. 2003 - Symptom Analysis of Chimeric Viruses between Wild and MM Isolates of Clover yellou vein virus
Yambao M.L.M.; Sasaki T.; Uyeda I.; Sato M.; Sekiguchi H.; Yagihashi H.; Takahashi Y.
Annals of the Phytopathological Society of Japan, 25 Feb. 2003, The Phytopathological Society of Japan (PSJ), English
25 Feb. 2003 - 25 Feb. 2003, Wild type Clover yellow vein virus (ClYVV) induces severe necrosis in broad bean plants. A spontaneous mutant (MM) that induces mosaic symptoms with replication efficiency similar to wild type was isolated. To determine the domain in the viral genome involve in necrosis induction, cDNAs to genomic RNA of MM mutant were exchanged with corresponding wild type fragment. Three chimeric clones, pClYVV/CN, pClYVV/NS and pClYVV/SS were made and inoculated to broad beans. Symptom severity was scored and viral accumulation was measured by western blot. pClYVV/NS and pClYVV/SS induced symptoms essentially the same as the wild type. Viral accumulation in broad beans after inoculation of these constructs are also similar to the wild type. On the other hand, pClYVV/CN containing the C-terminal P1 to N-terminal P3 of MM caused a drastic symptom change to mild chlorosis with debilitated infectivity. Sequence analysis of this MM regions showed amino acid substitutions at P1 (E182G), HCPro (T27I, I33V, R51I, D193Y, L327Q, V393I) and P3 (N122S). These results indicate that the ability to induce necrosis and the control of viral accumulation are mapped in the C-terminal P1 to N-terminal P3 region of ClYVV. - ClYVV Resistance of Common Bean cv. Jolanda Analyzed by Reporter Genes
Sato M.; Masuta C.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Feb. 2003, The Phytopathological Society of Japan (PSJ), Japanese
25 Feb. 2003 - 25 Feb. 2003, インゲンマメ品種Jolandaはクローバー葉脈黄化ウイルス(ClYVV)に対して抵抗性を示す.我々はgreen fluorescent protein(GFP,C3-S65T)を発現するpClYVV/C3-S65Tを構築し,その組み換えウイルスに対してもJolandaが抵抗性を示すことを確認した.pClYVV/C3-S65Tをパーティクルボンバードメントで導入した場合に感受性品種ではウイルス感染の進行と共にGFP蛍光は広がっていったが,Jolandaでは全く観察されなかった.この結果から,1細胞レベルで抵抗性が発揮されていることが明らかになった.次に感染性が失われた欠失クローンpClYVVΔSac/Gを大正金時およびJolandaに導入したところ両者でGFP蛍光は観察されたが,JolandaでのGFP蛍光発現に遅れが見られた.この遺伝子発現の遅れが感受性・抵抗性に関与するかを解析するために,ClYVVの非翻訳領域(NTR)とホタル由来のルシフェラーゼを連結した発現ベクターpClnLを用いた.このルシフェラーゼを用いた解析から,Jolandaの抵抗性とClYVVのNTRによる発現制御は無関係であることが判明した. - (225)The Genetic Background of Resistance to C1YVV in Jolanda Bean
Sato M.; Masuta C.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Aug. 2002, The Phytopathological Society of Japan (PSJ), Japanese
25 Aug. 2002 - 25 Aug. 2002 - Analysis of Clover yellow vein virus Mutant Can Overcome the Resistance in Common Bean Clover yellow vein virus
Sato M.; Masuta C.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Aug. 2001, The Phytopathological Society of Japan (PSJ), Japanese
25 Aug. 2001 - 25 Aug. 2001 - Modefication of Clover Yellow Vein Viral Expression Vector and Stability of the Expressed Gene(Abstracts Presented at the Meeting of the Hokkaido Division)
Wang Z.D.; Uyeda I.; Ueda S.; Sekiguchi H.; Takahashi Y.; Sato M.
Annals of the Phytopathological Society of Japan, 25 Dec. 2000, The Phytopathological Society of Japan (PSJ), Japanese
25 Dec. 2000 - 25 Dec. 2000 - Monitoring of Clover Yellow Vein Virus Infection by Green Fluorescent Protein (GFP) (Abstracts Presented at the Meeting of the Hokkaido Division)
Sato M.; Masuta C.; Uyeda I.
Annals of the Phytopathological Society of Japan, 25 Dec. 1999, The Phytopathological Society of Japan (PSJ), Japanese
25 Dec. 1999 - 25 Dec. 1999 - マメ科植物での高発現植物ウイルスベクターの構築
山名利一; 増田税; 高橋葉子; 佐藤昌直; 松村健; 上田一郎
日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 22 Jul. 1999, Japanese - Construction of a Plant Virus Based Vector and Highly Efficient Expression of a Foreign Gene in Legume Plants
Yamana T.; Masuta C.; Takahashi Y.; Sato M.; Matsumura T.; Uyeda E.
Annals of the Phytopathological Society of Japan, 25 Jun. 1999, The Phytopathological Society of Japan (PSJ), Japanese
25 Jun. 1999 - 25 Jun. 1999
- 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
- バイオテクノロジー学特論, 2024年, 修士課程, 農学院
- バイオテクノロジー学特論演習, 2024年, 修士課程, 農学院
- 応用分子生物学特論, 2024年, 修士課程, 農学院
- 応用分子生物学特論演習, 2024年, 修士課程, 農学院
- 応用分子昆虫学, 2024年, 学士課程, 農学部
- 応用生命科学実験, 2024年, 学士課程, 農学部
- 応用生命科学概論, 2024年, 学士課程, 農学部
- 基礎分子生物学, 2024年, 学士課程, 農学部
- 応用生命科学演習Ⅰ, 2024年, 学士課程, 農学部
- 農場実習, 2024年, 学士課程, 農学部
- Study of virus-mediated and viroid-mediated silencing of host plant genes
Grants-in-Aid for Scientific Research
29 Jun. 2018 - 31 Mar. 2021
Takeda Atsushi
To date, it is unclear how AGO1 and AGO2 determine the target mRNAs to be degraded in plants. To predict the target host mRNAs of AGO1-virus-derived siRNAs (vsiRNAs) and AGO2-vsiRNAs more precisely, I analyzed target specificities of AGO1-miRNA and AGO2-miRNA in plants. In case of AGO1-mediated silencing, I tested effects of G:U wobbles between miRNA and mRNAs. Results of a series of transient assay showed that G:U wobble has an intermediate effect between the base paring and the mismatch in a position-dependent manner. In case of AGO2-mediated silencing, I established a transient system to test the requirement of complementarities between miRNA and mRNAs by using a newly generated ago triple mutant of Nicotiana banthamiana. This system allows us to analyze mechanisms of the AGO2 specificity in plants. These basic analyses will help establish algorisms to predict host mRNAs targeted by AGO1-vsiRNAs and AGO2-vsiRNAs in future.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Ritsumeikan University, 18K19219 - New approach for studying functional importance of baculovirus nonessential genes
Grants-in-Aid for Scientific Research
01 Apr. 2015 - 31 Mar. 2018
Bando Hisanori; TAKA Hitomi; ISHIBASHI Taiki; HARADA Naoto; SAITO Ryo
In Bombyx mori nucleopolyhedrovirus (BmNPV) genome comprised of 143 genes, more than 60 % of them are nonessential genes for efficient multiplication in Bombyx mori cell line BmN. However, a combination of nonessential genes was found to play a critical role in efficient viral multiplication, suggested essentiality of nonessential genes as a collected gene. A study using reverse genetics identified genetic interactions among nonessential gens in such a combination and emphasized importance of a methodology to advance the reverse combinatorial genetics of BmNPVs; thus we established a platform for BmNPV genome assembly from PCR-amplified DNA fragments using Gibson Assembly for further analysis of the essentiality of nonessential genes and for reconstruction of BmNPV vector system based on logical design.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 15H04608 - Molecular basis of formation and regulation of insect holocentric chromosome
Grants-in-Aid for Scientific Research
01 Apr. 2014 - 31 Mar. 2018
KUSAKABE TAKAHIRO
Lepidopteran insects are known to have specific chromosomes called heliocentric chromosome having multiple centromeres along the entire length. In this study, we clarified the molecular basis of kinetochore formation of the heliocentric chromosome and newly found six centromere-related genes specific to silkworm using the gene network analysis. Based on these results and analysis on replication related genes, an artificial chromosome functioning by tethering DNA replication factor and kinetochore protein was synthesized.
On the other hand, as for the genome-wide gene expression regulation and molecular basis of chromatin structure, we focused on histone acetylation and deacetylation enzymes, and performed gene knockdown on these genes. As a result, we found that HDAC 8 plays an important role in the silkworm.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Kyushu University, 26252057 - Functional genomics on in vivo fitness of BmNPV
Grants-in-Aid for Scientific Research
01 Apr. 2015 - 31 Mar. 2017
Hisanori Bando; ASANO SHINICHIRO
Bombyx mori nucleopolyhedrovirus (BmNPV) H4 that had genome sharing high sequence similarity (approx. 97%) with that of the type strain of BmNPV T3 showed marked growth advantage in silkworm larvae. Studies using chimeric recombinant BmNPV between H4 and T3 demonstrated that a viral membrane protein gene gp64 as a determinant in the in vivo fitness of BmNPV H4. Further analyses identified amino acid positions involved in the BmNPV H4 phenotype of efficient growth in silkworm larvae. These results suggested the possibility to control growth ability of BmNPV in silkworm larvae by gp64-targetted genome manipulation.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 15K14891 - Studies of the small RNA specificity and the viral pathogenicity in plants
Grants-in-Aid for Scientific Research
01 Apr. 2015 - 31 Mar. 2017
Takeda Atsushi; SATO MASANAO
In plants, viral infection induces RNA silencing, which is a basal immune response against viruses. In the anti-viral pathways of plant RNA silencing, abundant viral-derived small-interfering RNAs (vsiRNAs) are generated through functions of Dicer-like enzymes (DCLs). A class of 21 nt vsiRNAs cleaved by DCL4 is incorporated into Argonaute 1 (AGO1). The AGO1-vsiRNA complex has a potential to cleave not only viral RNAs but also endogenous mRNAs dependent on the complementarity between target RNAs and vsiRNAs in AGO1. However, determinants of target specificity of AGO1-vsiRNA complex are unknown and thus we cannot predict the target mRNAs downregulated upon viral infection. In this study, we examined the AGO1 specificity in plants experimentally by using a massive transient luciferase assay. We identified several determinants of the plant AGO1 specificity, which can help predict the target mRNAs of vsiRNAs in viral-infected plants in future.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Ritsumeikan University, 15K14665 - Development of en masse inter-species genetic interaction analysis using a pooled E. coli gene knockout strans
Grants-in-Aid for Scientific Research
01 Apr. 2015 - 31 Mar. 2017
Sato Masanao
Microbes and hosts have been co-evolved as they have repeatedly developed a better strategy to infect/defend in infection processes and to counter it. Such coevolution processes result in development of highly complex gene network in both host and microbes. To elucidate the gene network in host or microbe for their interaction, high throughput molecular profiling and genetic interaction analyses have identified thousands of genes in host or microbe. A major next challenge in research on host-microbe interactions is to elucidate interactions between host and microbial gene networks.
In this study, I aimed at development of a method to efficiently collect genetic information on how host and microbial gene networks interact in an seamlessly integrated manner. The developed protocol and data analysis pipeline accurately collect readouts in an inter-species genetic interactions en masse.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, 15K14437 - Study of population dynamics of host population during viral infection
Grants-in-Aid for Scientific Research
01 Apr. 2014 - 31 Mar. 2017
Ueki Shoko; Higashi Aiko; Kobashi Rieko
The initial goal of the project was to analyze the infection process of Heterosigma akashiwo virus (HaV) to its host population, bloom-forming algae, Heterosigma akashiwo, that consisted from strains showing the different reactions to HaV infection. During the project, we completed the whole genome sequencing and gene predictions of HaV, and obtained the insights into the evolutionary and phylogenetical position to other giant double-stranded DNA viruses. In addition, contradicting to the initial understanding, we found out that the host reactions to HaV infection were not only determined by the host genotypes, but also by the presence/absence or the species of the accomplice bacteria associating to the host. The project developed into direction unpredicted when it was initially planed: however, the insights obtained by the project will provide infrastructure for future studies about H. akashiwo and its virus from cellular and molecular biology view points.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Okayama University, 26291092 - Assembly of baculovirus genome DNA fragments in yeast for improving baculovectors
Grants-in-Aid for Scientific Research
01 Apr. 2013 - 31 Mar. 2015
BANDO Hisanori; SATO Masanao
The technologies that enable flexible manipulation of virus genome are very important technical base for improving viral vectors. The aim of this study is to establish a method for assembling baculovirus genome DNA from both synthetic and natural DNA fragments to improve baculovectors. First, we constructed a baculovirus of the silkworm (BmNPV/bacmid) carrying an yeast replication origin (Yori). Second, we cloned 21 overlapping DNA fragments covering whole sequence of the BmNPV/bacmid genome DNA with Yori in bacterial cells using a plasmid vector. Lastly, we assembled the 21 fragments in yeast to form the complete BmNPV genome DNA.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 25660260 - A systems biology approach for improving baculovector system
Grants-in-Aid for Scientific Research
01 Apr. 2010 - 31 Mar. 2014
BANDO Hisanori; KUSAKABE Takahiro; SATOU Masanao
By taking a systems biology approach, a baculovirus gene network models was built and host factors involved in recombinant protein expression from a baculovector system were identified.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 22248003 - A systems approach to elucidate gene regulatory network for gernline development and fertility
Grants-in-Aid for Scientific Research
2011 - 2013
SATO Masanao
The signaling network in a germline cell was modeled using BmN4, the cell line derived from a Bombyx mori ovary. The network model is composed of genes that are evolutionarily conserved between B. mori and Drosophila melanogaster and that have been known as regulators for signal transduction in D. melanogaster germline or somatic cell development. The model suggested a gene known as a regulator of germline development in D. melanogaster as a hub in the network. In addition, the model inferred roles of genes of which mutations caused sterility by unknown reasons. Furthermore, the model inferred roles of genes, which have been known to function in somatic cells, in germline cells.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), National Institute for Basic Biology, 23710237 - Mechanism regulating GSC niche system in Drosophila ovary and testis
Grants-in-Aid for Scientific Research
2008 - 2012
KOBAYASHI Satoru; ASAOKA Miho; HAYASHI Yoshiki; SATO Masanao; KITADATE Yu; ABE Kuniya
In this study, we have been trying to clarify the mechanisms regulating "GSC Niche System" in Drosophila. We found that 1) heparan sulphate proteoglycans (HSPG) are deeply involved in GSC maintenance by restricting the function of growth factors in a narrow region (Niche field) around "Niche cells", 2) Notch, Sevenless and Egfr signaling are required for proper formation of "Niche cells" in the anterior region of male embryonic gonads, and that 3) maternal Ovo and Sxl are required for germline-specific gene expression and sex determination of primordial germ cells, respectively.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), National Institutes of Natural Sciences Okazaki Research Facilities, 20116002
