Researcher basic information

• 博士（水産科学）, 北海道大学

• https://researchmap.jp/y-kuma3?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201301024085045609

• 201301024085045609

• carbohydrate relating enzyme
• mycosporine like amino acids
• brown algae
• タンパク質工学
• 酵素利用
• 紅藻類

• Life Science, Aquatic life science, 水産資源開発工学

• Bachelor's degree program, School of Fisheries Sciences
• Master's degree program, Graduate School of Fisheries Sciences
• Doctoral (PhD) degree program, Graduate School of Fisheries Sciences

Research activity information

• Nov. 2025, キャンパスコンソーシアム函館, 大賞（ステージセッション）
  フコイダンによる血液凝固の抑制と促進～魚類と哺乳類で異なる働き～
  長澤和貴;青木建斗;曽我英利;辻井豪佑;太田龍成;岸村 栄毅;熊谷 祐也
• Nov. 2024, 14th JOINT INTERNATIONAL SYMPOSIUM ON FOOD SCIENCE AND TECHNOLOGY, BEST PRESENTATION AWARD
  Sustainable Livestock Production Enabled by Fisheries Waste
  Gosuke Tsujii;Yuya Kumagai;Hideki Kishimura
• Nov. 2024, キャンパスコンソーシアム函館, 優秀賞（ステージセッション）
  低利用資源「海のパセリ」のここがすごい！ ～糖尿病予防に寄与する可能性を明らかに～
  太田 龍成;馬場 惇平;石川 勇斗;岸村 栄毅;熊谷 祐也
• Sep. 2024, 公益社団法人日本缶詰びん詰レトルト食品協会, 令和 6 年度年度「逸見賞」
  海藻レトルト食品の栄養成分および抗酸化 力に及ぼす製造条件の影響
  宮部好克;落合瞳子;熊谷祐也;岸村栄毅
• Nov. 2022, キャンパス・コンソーシアム函館, ブースセッション『優秀賞』
  色落ちダルスに救済を!
  濵﨑海瑠;辰巳 幸彦;岸村栄毅;熊谷祐也
• Dec. 2021, キャンパス・コンソーシアム函館, ブースセッション『大賞』
  海藻のヌメヌメ成分「フコイダン」の最大Maxパワー力を生かすために
  松井亘;伊藤大翔;熊谷祐也;岸村栄毅
• Dec. 2021, キャンパス・コンソーシアム函館, ステージセッション『優秀賞 (産学連携「クリエイティブネットワーク」賞) 』
  季節変動から紐解くダルス「MAAs」産業利用への試み
  前田侑也;山本竜矢;熊谷祐也;岸村栄毅
• Nov. 2020, キャンパス・コンソーシアム函館, ブースセッション『優秀賞』
  ダルスで腸を元気にする！
  吉田光里;小林真奈美;栗田大輝;熊谷祐也;岸村栄毅
• Nov. 2020, キャンパス・コンソーシアム函館, ブースセッション『優秀賞』
  ミッション！低利用海藻に眠るお宝「MAAs」を可能な限り手に入れよ！
  杉山佳奈海;西田有輝;熊谷祐也;岸村栄毅
• Nov. 2020, Food Society of Modern International Lifestyle Education, Good Oral Award
  Efficient Extraction and Monthly Variation of Mycosporine-like Amino Acids from Red Alga Dulse in Japan
  ○Yuki Nishida;Yuya Kumagai;Shunta Michiba;Hajime Yasui;Hideki Kishimura
• Nov. 2019, キャンパス・コンソーシアム函館「アカデミックリンク2019」大賞 海藻の秘密兵器！？「マイコスポリン様アミノ酸」
  西田有輝;熊谷祐也;岸村英毅
• Nov. 2018, キャンパス・コンソーシアム函館 ブース部門『優秀賞』「絶対に捨てられない海藻がここにある」
  小林真奈美;山本陽平;熊谷祐也;岸村栄毅
• Sep. 2013, 日本応用糖質科学, 日本応用糖質科学会平成25年度大会（第62回）ポスター賞
  Gluconobacter oxydans由来dextran dextrinaseによる直鎖イソマルトメガロ糖の酵素合成
  熊谷祐也;Weeranuch Lang;貞廣樹里;奥山正幸;森 春英;木村淳夫
• Feb. 2012, 日本応用糖質科学北海道支部, 平成23年度日本応用糖質学会北海道支部・支部奨励賞
  水産軟体動物由来ラミナリナーゼの酵素特性と環境適応に関する研究
  熊谷祐也

• Atlantic salmon skin gelatin hydrolysate: Antioxidant activity and functional role as a cryoprotectant in freeze-thawed surimi
  Sakonwat Kuepethkaew; Soottawat Benjakul; Yuya Kumagai; Hideki Kishimura; Sappasith Klomklao
  International Journal of Refrigeration, 182, 533, 545, Elsevier BV, Feb. 2026
  Scientific journal
• Structural basis of transglucosylation in dextran dextrinase, a homolog of anomer-inverting GH15 glucoside hydrolases
  Takayoshi Tagami; Wataru Saburi; Juri Sadahiro; Yuya Kumagai; Weeranuch Lang; Naohiro Matsugaki; Masayuki Okuyama; Haruhide Mori; Atsuo Kimura
  Journal of Biological Chemistry, 110541, 110541, Elsevier BV, Jul. 2025, [Peer-reviewed]
  English, Scientific journal
• Mycosporine-like amino acids from red alga Devaleraea inkyuleei harvested at two locations in southern Hokkaido
  Koki Takizawa; Hideki Kishimura; Shingo Akita; Yuya Kumagai
  Fisheries Science, Springer Science and Business Media LLC, 27 Jun. 2025, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• A Comparative Study of Different Precipitation Techniques for Protein Isolation from Cordyceps militaris: Effects on Functional and Structural Properties
  Natthapong Mungmueang; Theeraphol Senphan; Wichittra Daengprok; Chitraporn Ngampeerapong; Mongkol Thirabunyanon; Soottawat Benjakul; Hideki Kishimura; Yuya Kumagai; Chodsana Sriket
  Food Biophysics, 20, 1, Springer Science and Business Media LLC, 08 Mar. 2025, [Peer-reviewed]
  Scientific journal
• Molecular structure of enzyme-synthesized amylose-like chimeric isomaltomegalosaccharides and their encapsulation of the sulfasalazine prodrug
  Weeranuch Lang; Yoshiaki Yuguchi; Chun-Yao Ke; Ting-Wei Chang; Yuya Kumagai; Wilaiwan Kaenying; Takayoshi Tagami; Feng Li; Takuya Yamamoto; Kenji Tajima; Kenji Takahashi; Takuya Isono; Toshifumi Satoh; Atsuo Kimura
  Carbohydrate Polymers, 122956, 122956, Elsevier BV, Nov. 2024, [Peer-reviewed]
  English, Scientific journal
• DEEP-SEA FISHES COLLECTED FROM THE ANDAMAN SEA BY R/V CHAKRATONG TONGYAI DURING 1996–2000. PART 9: ORDERS CHIMAERIFORMES, CARCHARHINIFORMES, TORPEDINIFORMES AND RAJIFORMES
  Toshio Kawai; Fumihito Tashiro; Naohide Nakayama; Yuya Kumagai; Charatsee Aungtonya; Surangkana Tuanapay
  Phuket mar. biol. Cent. Res. Bull., 81, 93, 104, Sep. 2024, [Peer-reviewed]
  English, Scientific journal
• Extraction and antioxidant capacity of mycosporine-like amino acids from red algae in Japan.
  Ryuya Yamamoto; Shigeru Toriumi; Chikara Kawagoe; Wataru Saburi; Hideki Kishimura; Yuya Kumagai
  Bioscience, biotechnology, and biochemistry, 29 Apr. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Mycosporine-like amino acids (MAAs) are the natural UV absorbing compounds with antioxidant activity found in microalgae and macroalgae. We collected red algae Asparagopsis taxiformis, Meristotheca japonica, and Polysiphonia senticulosa from Nagasaki, where UV radiation is more intense than in Hokkaido, and investigated the effect of UV radiation on MAAs content. It was suggested that A. taxiformis and M. japonica contained shinorine and palythine, while UV absorbing compound in P. senticulosa could not be identified. The amounts of these MAAs were lower compared to those from Hokkaido. Despite an increase in UV radiation in both region from February to April, MAAs contents of red algae from Nagasaki slightly decreased, while that from Hokkaido significantly decreased. This difference was suggested the amount of inorganic nitrogen in the ocean. Antioxidant activity of MAAs increased under alkaline conditions. The extract containing MAAs from P. senticulosa showed the highest antioxidant activity among four red algae.
• Understanding Antidiabetic Potential of Oligosaccharides from Red Alga Dulse Devaleraea inkyuleei Xylan by Investigating α-Amylase and α-Glucosidase Inhibition
  Martin Alain Mune Mune; Tadashi Hatanaka; Hideki Kishimura; Yuya Kumagai
  Molecules, 29, 7, 1536, 1536, MDPI AG, 29 Mar. 2024, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, In this study, the α-glucosidase (maltase-glucoamylase: MGAM) and α-amylase inhibitory properties elicited by xylooligosaccharides (XOSs) prepared from dulse xylan were analysed as a potential mechanism to control postprandial hyperglycaemia for type-2 diabetes prevention and treatment. Xylan was purified from red alga dulse powder and used for enzymatic hydrolysis using Sucrase X to produce XOSs. Fractionation of XOSs produced xylobiose (X2), β-(1→3)-xylosyl xylobiose (DX3), xylotriose (X3), β-(1→3)-xylosyl-xylotriose (DX4), and a dulse XOS mixture with n ≥ 4 xylose units (DXM). The different fractions exhibited moderate MGAM (IC50 = 11.41–23.44 mg/mL) and α-amylase (IC50 = 18.07–53.04 mg/mL) inhibitory activity, which was lower than that of acarbose. Kinetics studies revealed that XOSs bound to the active site of carbohydrate digestive enzymes, limiting access to the substrate by competitive inhibition. A molecular docking analysis of XOSs with MGAM and α-amylase clearly showed moderate strength of interactions, both hydrogen bonds and non-bonded contacts, at the active site of the enzymes. Overall, XOSs from dulse could prevent postprandial hyperglycaemia as functional food by a usual and continuous consumption.
• Tunable structure of chimeric isomaltomegalosaccharides with double α-(1 → 4)-glucosyl chains enhances the solubility of water-insoluble bioactive compounds
  Weeranuch Lang; Takayoshi Tagami; Yuya Kumagai; Seiya Tanaka; Hye-Jin Kang; Masayuki Okuyama; Wataru Saburi; Haruhide Mori; Tohru Hira; Chaehun Lee; Takuya Isono; Toshifumi Satoh; Hiroshi Hara; Takayuki Kurokawa; Nobuo Sakairi; Yoshiaki Yuguchi; Atsuo Kimura
  Carbohydrate Polymers, 319, 121185, 121185, Elsevier BV, Nov. 2023, [Peer-reviewed]
  Scientific journal
• Mycosporine-like Amino Acids from Red Alga Dulse (Devaleraea inkyuleei): Monthly Variation and Improvement in Extraction
  Ryuya Yamamoto; Koki Takizawa; Yoshikatsu Miyabe; Martin Alain Mune Mune; Hideki Kishimura; Yuya Kumagai
  Phycology, 3, 3, 394, 404, MDPI AG, 07 Sep. 2023, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, Mycosporine-like amino acids (MAAs) are natural UV-absorbing compounds found in microalgae and macroalgae. The content of MAAs in algae varies with the seasons and environmental factors. Red alga dulse in Usujiri (Hokkaido, Japan) is an underutilized resource. Therefore, we investigated the amount of MAAs in Usujiri dulse in 2022 to clarify the suitable months for MAA extraction. In addition, we also evaluated the extraction method focusing on the extraction volume. MAAs were prepared via the 20 volumes of 25% ethanol extraction method and detected via HPLC. The results showed that the amount of MAAs on 25 March 2022 showed the highest value (40.4 μmol/g DW) among the samples from 24 January to 13 May. The tendency of suitable samples for MAA preparation corresponded to the term from mid-February to early April, which was the same as the previous three years. Although the surveys from 2019–2021 were performed by using the successive water–methanol method, it was found that the improved method also reflected the monthly variation in MAAs. The extraction of MAAs was performed via 20 or 40 volumes of 25% ethanol at 4 °C for 24 h. The amount of MAAs with 40 volumes of 25% ethanol extraction increased 1.3-fold compared to that with 20 volumes of 25% ethanol extraction. These data are useful information for valuable compound extraction from Usujiri dulse.
• 海藻レトルト食品の栄養成分および抗酸化力に及ぼす製造条件の影響
  宮部好克; 落合瞳子; 熊谷祐也; 岸村栄毅
  日本調理科学会誌, Jun. 2023, [Peer-reviewed]
  Japanese, Scientific journal
• Exploring Bioactive Compounds in Brown Seaweeds Using Subcritical Water: A Comprehensive Analysis
  Jin-Seok Park; Ji-Min Han; Yu-Na Shin; Ye-Seul Park; Ye-Ryeon Shin; Sin-Won Park; Vikash Chandra Roy; Hee-Jeong Lee; Yuya Kumagai; Hideki Kishimura; Byung-Soo Chun
  Marine Drugs, 21, 6, 328, 328, MDPI AG, 26 May 2023
  Scientific journal, In this study, we characterized the bioactive properties of three important brown seaweed species, Sargassum thunbergii, Undaria pinnatifida, and Saccharina japonica, by subcritical water extraction (SWE), as these species are well known for their beneficial health effects. Their physiochemical properties, including potential antioxidant, antihypertensive, and α-glucosidase inhibitory activity, and the antibacterial activity of the hydroysates were also analyzed. The highest total phlorotannin, total sugar content, and reducing sugar content in the S. thunbergii hydrolysates were 38.82 ± 0.17 mg PGE/g, 116.66 ± 0.19 mg glucose/g dry sample, and 53.27 ± 1.57 mg glucose/g dry sample, respectively. The highest ABTS+ and DPPH antioxidant activities were obtained in the S. japonica hydrolysates (124.77 ± 2.47 and 46.35 ± 0.01 mg Trolox equivalent/g, respectively) and the highest FRAP activity was obtained in the S. thunbergii hydrolysates (34.47 ± 0.49 mg Trolox equivalent/g seaweed). In addition, the seaweed extracts showed antihypertensive (≤59.77 ± 0.14%) and α-glucosidase inhibitory activity (≤68.05 ± 1.15%), as well as activity against foodborne pathogens. The present findings provide evidence of the biological activity of brown seaweed extracts for potential application in the food, pharmaceutical, and cosmetic sectors.
• Monthly Variation in Mycosporine-like Amino Acids from Red Alga Dulse (Devaleraea inkyuleei, Formerly Palmaria palmata in Japan)
  Ryuya Yamamoto; Martin Alain Mune Mune; Yoshikatsu Miyabe; Hideki Kishimura; Yuya Kumagai
  Phycology, 3, 1, 127, 137, MDPI AG, 09 Feb. 2023, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, Mycosporine-like amino acids (MAAs) are natural ultraviolet-absorbing compounds found in microalgae and macroalgae. MAA content changes seasonally and in response to environmental factors. We previously investigated MAAs from the red alga dulse (Devaleraea inkyuleei, formerly Palmaria palmata in Japan) in Usujiri, Hokkaido, Japan, from 2019 to 2020. At that time, some factors affecting MAA content were still unclear. In this study, we investigated MAA variation during the period from January to June 2021, and evaluated new methods of MAA extraction from dulse. We recorded a maximum MAA extraction yield (7.03 µmol/g dry weight) on 25 March 2021. Over the course of our three years of investigations from 2019 to 2021, we found that dulse was most suitable for MAA preparation from the middle of February to late April. In the later work reported in this paper, we improved our extraction method by using a lower-risk organic solvent (ethanol) rather than methanol. In addition, we evaluated MAA extraction using different levels of ethanol concentration (25, 50, and 99%) and different extraction times (2, 6, and 24 h). We found that extraction with 25% ethanol for 24 h increased MAA content by a factor of 3.2, compared with our previous extraction method. In summary, we determined the most suitable sampling period for Usujiri dulse, to extract the highest content of MAAs. We also improved the effectiveness of the extraction process.
• Characterisation of Bioactive Peptides from Red Alga Gracilariopsis chorda
  Martin Alain Mune Mune; Yoshikatsu Miyabe; Takeshi Shimizu; Wataru Matsui; Yuya Kumagai; Hideki Kishimura
  Marine Drugs, 21, 1, 49, 49, MDPI AG, 11 Jan. 2023, [Peer-reviewed]
  English, Scientific journal, In this study, we studied the bioactive peptides produced by thermolysin hydrolysis of a water-soluble protein (WSP) from the red alga Gracilariopsis chorda, whose major components are phycobiliproteins and Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCo). The results showed that WSP hydrolysate exhibited significantly higher ACE inhibitory activity (92% inhibition) compared to DPP-IV inhibitory activity and DPPH scavenging activity. The phycobiliproteins and RuBisCo of G. chorda contain a high proportion of hydrophobic (31.0–46.5%) and aromatic (5.1–46.5%) amino acid residues, which was considered suitable for the formation of peptides with strong ACE inhibitory activity. Therefore, we searched for peptides with strong ACE inhibitory activity and identified two novel peptides (IDHY and LVVER). Then, their interaction with human ACE was evaluated by molecular docking, and IDHY was found to be a promising inhibitor. In silico analysis was then performed on the structural factors affecting ACE inhibitory peptide release, using the predicted 3D structures of phycobiliproteins and RuBisCo. The results showed that most of the ACE inhibitory peptides are located in the highly solvent accessible α-helix. Therefore, it was suggested that G. chorda is a good source of bioactive peptides, especially ACE-inhibitory peptides.
• Editorial: Application of biotechnology on the high-value development of marine bioresource
  Zedong Jiang; Haijin Mou; Yuya Kumagai; Hideki Kishimura
  Frontiers in Bioengineering and Biotechnology, 10, Frontiers Media SA, Oct. 2022, ［Internationally co-authored］, [International Magazine]
  English, Scientific journal
• Physicochemical functionality of chimeric isomaltomegalosaccharides with α-(1 → 4)-glucosidic segments of various lengths
  Weeranuch Lang; Yuya Kumagai; Shinji Habu; Juri Sadahiro; Takayoshi Tagami; Masayuki Okuyama; Shinichi Kitamura; Nobuo Sakairi; Atsuo Kimura
  Carbohydrate Polymers, 291, 119562, 119562, Elsevier BV, Sep. 2022, [Peer-reviewed]
  English, Scientific journal
• Characterization of an Unknown Region Linked to the Glycoside Hydrolase Family 17 β-1,3-Glucanase of Vibrio vulnificus Reveals a Novel Glucan-Binding Domain.
  Yuya Kumagai; Hideki Kishimura; Weeranuch Lang; Takayoshi Tagami; Masayuki Okuyama; Atsuo Kimura
  Marine drugs, 20, 4, 31 Mar. 2022, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, The glycoside hydrolase family 17 β-1,3-glucanase of Vibrio vulnificus (VvGH17) has two unknown regions in the N- and C-termini. Here, we characterized these domains by preparing mutant enzymes. VvGH17 demonstrated hydrolytic activity of β-(1→3)-glucan, mainly producing laminaribiose, but not of β-(1→3)/β-(1→4)-glucan. The C-terminal-truncated mutants (ΔC466 and ΔC441) showed decreased activity, approximately one-third of that of the WT, and ΔC415 lost almost all activity. An analysis using affinity gel containing laminarin or barley β-glucan revealed a shift in the mobility of the ΔC466, ΔC441, and ΔC415 mutants compared to the WT. Tryptophan residues showed a strong affinity for carbohydrates. Three of four point-mutations of the tryptophan in the C-terminus (W472A, W499A, and W542A) showed a reduction in binding ability to laminarin and barley β-glucan. The C-terminus was predicted to have a β-sandwich structure, and three tryptophan residues (Trp472, Trp499, and Trp542) constituted a putative substrate-binding cave. Linker and substrate-binding functions were assigned to the C-terminus. The N-terminal-truncated mutants also showed decreased activity. The WT formed a trimer, while the N-terminal truncations formed monomers, indicating that the N-terminus contributed to the multimeric form of VvGH17. The results of this study are useful for understanding the structure and the function of GH17 β-1,3-glucanases.
• Introduction of a second "Green Revolution" mutation into wheat via in planta CRISPR/Cas9 delivery.
  Yuya Kumagai; Yuelin Liu; Haruyasu Hamada; Weifeng Luo; Jianghui Zhu; Misa Kuroki; Yozo Nagira; Naoaki Taoka; Etsuko Katoh; Ryozo Imai
  Plant physiology, 188, 4, 1838, 1842, 28 Mar. 2022, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal, Direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem via particle bombardment enabled introduction of a semidwarf1-orthologous mutation into an elite wheat variety.
• Characterization of Antioxidant Activity of Heated Mycosporine-like Amino Acids from Red Alga Dulse Palmaria palmata in Japan.
  Yuki Nishida; Wataru Saburi; Yoshikatsu Miyabe; Hideki Kishimura; Yuya Kumagai
  Marine drugs, 20, 3, 01 Mar. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, We recently demonstrated the monthly variation and antioxidant activity of mycosporine-like amino acids (MAAs) from red alga dulse in Japan. The antioxidant activity of MAAs in acidic conditions was low compared to that in neutral and alkali conditions, but we found strong antioxidant activity from the heated crude MAA fraction in acidic conditions. In this study, we identified and characterized the key compounds involved in the antioxidant activity of this fraction. We first isolated two MAAs, palythine, and porphyra-334, from the fraction and evaluated the activities of the two MAAs when heated. MAAs possess absorption maxima at around 330 nm, while the heated MAAs lost this absorption. The heated MAAs showed a high ABTS radical scavenging activity at pH 5.8-8.0. We then determined the structure of heated palythine via ESI-MS and NMR analyses and speculated about the putative antioxidant mechanism. Finally, a suitable production condition of the heated compounds was determined at 120 °C for 30 min at pH 8.0. We revealed compounds from red algae with antioxidant activities at a wide range of pH values, and this information will be useful for the functional processing of food.
• Physicochemical properties of house and cave edible bird's nest from Southern Thailand
  Kasidate Chantakun; Hideki Kishimura; Yuya Kumagai; Soottawat Benjakul
  ScienceAsia, 48, 2, 136, 136, Science Society of Thailand, Feb. 2022, [Peer-reviewed]
  English, Scientific journal
• A practical approach to producing isomaltomegalosaccharide using dextran dextrinase from Gluconobacter oxydans ATCC 11894.
  Weeranuch Lang; Yuya Kumagai; Juri Sadahiro; Wataru Saburi; Rakrudee Sarnthima; Takayoshi Tagami; Masayuki Okuyama; Haruhide Mori; Nobuo Sakairi; Doman Kim; Atsuo Kimura
  Applied microbiology and biotechnology, 106, 2, 689, 698, Jan. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Dextran dextrinase (DDase) catalyzes formation of the polysaccharide dextran from maltodextrin. During the synthesis of dextran, DDase also generates the beneficial material isomaltomegalosaccharide (IMS). The term megalosaccharide is used for a saccharide having DP = 10-100 or 10-200 (DP, degree of polymerization). IMS is a chimeric glucosaccharide comprising α-(1 → 6)- and α-(1 → 4)-linked portions at the nonreducing and reducing ends, respectively, in which the α-(1 → 4)-glucosyl portion originates from maltodextrin of the substrate. In this study, IMS was produced by a practical approach using extracellular DDase (DDext) or cell surface DDase (DDsur) of Gluconobacter oxydans ATCC 11894. DDsur was the original form, so we prepared DDext via secretion from intact cells by incubating with 0.5% G6/G7 (maltohexaose/maltoheptaose); this was followed by generation of IMS from various concentrations of G6/G7 substrate at different temperatures for 96 h. However, IMS synthesis by DDext was limited by insufficient formation of α-(1 → 6)-glucosidic linkages, suggesting that DDase also catalyzes elongation of α-(1 → 4)-glucosyl chain. For production of IMS using DDsur, intact cells bearing DDsur were directly incubated with 20% G6/G7 at 45 °C by optimizing conditions such as cell concentration and agitation efficiency, which resulted in generation of IMS (average DP = 14.7) with 61% α-(1 → 6)-glucosyl content in 51% yield. Increases in substrate concentration and agitation efficiency were found to decrease dextran formation and increase IMS production, which improved the reaction conditions for DDext. Under modified conditions (20% G6/G7, agitation speed of 100 rpm at 45 °C), DDext produced IMS (average DP = 14.5) with 65% α-(1 → 6)-glucosyl content in a good yield of 87%. KEY POINTS: • Beneficial IMS was produced using thermostabilized DDase. • Optimum conditions for reduced dextran formation were successfully determined. • A practical approach was established to provide IMS with a great yield of 87%.
• Enzymological characteristics of pepsinogens and pepsins purified from lizardfish (Saurida micropectoralis) stomach
  Sakonwat Kuepethkaew; Yi Zhang; Hideki Kishimura; Yuya Kumagai; Benjamin K. Simpson; Soottawat Benjakul; Srinivasan Damodaran; Sappasith Klomklao
  Food Chemistry, 366, 130532, 130532, Elsevier BV, Jan. 2022, [Peer-reviewed]
  English, Scientific journal
• Preparation of β(1→3)/β(1→4) xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseus
  Yuki Fujii; Manami Kobayashi; Yoshikatsu Miyabe; Hideki Kishimura; Tadashi Hatanaka; Yuya Kumagai
  Bioresources and Bioprocessing, 8, 1, 38, Springer Science and Business Media LLC, Dec. 2021, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, Red alga dulse contains xylan with β(1→3)/β(1→4) linkages. We previously prepared xylooligosaccharides (XOSs) from dulse xylan; however, the product contained many d-xylose residues and fewer XOSs with β(1→3) linkages. To improve the efficiency of XOS production, we prepared two recombinant endoxylanases from Streptomyces thermogriseus (StXyl10 and StXyl11). Comparing the k_cat/K_m values for dulse xylan, this value from StXyl10 was approximately two times higher than that from StXyl11. We then determined the suitable conditions for XOS production. As a result, dulse XOS was prepared by the successive hydrolysis of 10 mg/mL dulse xylan by 0.5 μg/mL StXyl10 for 4 h at 50 °C and then 2.0 μg/mL StXyl11 for 36 h at 60 °C. Xylan was converted into 95.8% XOS, including 59.7% XOS with a β(1→3) linkage and 0.97% d-xylose. Our study provides useful information for the production of XOSs with β(1→3) linkages.
• Monthly Variation and Ultraviolet Stability of Mycosporine-like Amino Acids from Red Alga Dulse Palmaria palmata in Japan
  Yuki Nishida; Yoshikatsu Miyabe; Hideki Kishimura; Yuya Kumagai
  Phycology, 1, 2, 119, 128, MDPI AG, 25 Nov. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Mycosporine-like amino acids (MAAs) are the natural ultraviolet (UV)-absorbing compounds from micro- and macro-algae. The MAAs in algae change with the environmental conditions and seasons. We previously determined an efficient extraction method of MAAs from red alga dulse in Usujiri (Hokkaido, Japan) and revealed monthly variation of MAA in 2019. Dulse samples in 2019 for MAA preparation were suitable from late February to April. In this study, to confirm the suitable timings to extract MAAs from Usujiri dulse, we also investigated the monthly (from January to May) variation of MAA content in 2020. There were the most MAAs in the sample on 18 March (6.696 µmol g−1 dry weight) among the samples from January to May 2020. From two years of investigation, we deduce that samples of Usujiri dulse from late February to early April were suitable for MAA preparation. The UV stability of the two major purified MAAs in Usujiri dulse—palythine and porphyra-334—was tested. The two MAAs and 2-hydroxy-4-methoxybenzophenone were stable for up to 12 h under a 312 nm lamp at 200 µW cm−2, but 2-ethylhexyl-4-methoxycinnamate formed a cis/trans-mixture in a short time. The data in this study show the suitable sampling period for Usujiri dulse and the possible application for UV protection from food and cosmetics.
• 水産加工食品のω3脂肪酸残存率に及ぼす製造条件の影響
  宮部好克; 落合瞳子; 熊谷祐也; 岸村栄毅
  日本調理科学会誌, 一般社団法人 日本調理科学会, Nov. 2021, [Peer-reviewed]
  Japanese, Scientific journal
• Study on the Mechanism of the Blood-Glucose-Lowering Effect of Collagen Peptides from Sturgeon By-Products
  Yukiho Sasaoka; Taichi Takagi; Shunta Michiba; Yohei Yamamoto; Yuya Kumagai; Hideki Kishimura
  Marine Drugs, 19, 10, 584, 584, MDPI AG, 19 Oct. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, In a previous study, we found that the collagen peptides prepared from the by-products of Bester sturgeon had an inhibitory effect on elevated blood glucose levels in a glucose tolerance test with ICR mice. In the present study, we examine the mechanism of the effect of sturgeon collagen peptides (SCPs) in detail. When glucose was orally administered to mice along with the SCPs, it was found that the glucose remained in the stomach for a longer time. In the above tests, the amount of glucose excreted in the feces of mice also increased. On the contrary, it was revealed that the SCPs have a dipeptidyl-peptidase-IV (DPP-IV) inhibitory ability in an in vitro test. In subsequent oral and intravenous glucose administration tests, glucagon-like peptide-1 (GLP-1) and insulin levels in the blood of mice were maintained at high levels. These results suggested the following three mechanisms: SCPs slow the rate of transportation of glucose from the stomach into the small intestine, resulting in delayed glucose absorption; SCPs suppress the absorption of glucose in the small intestine and excrete it from the body; SCPs inhibit DPP-IV in the blood and maintain a high GLP-1 level in blood, which in turn stimulates insulin secretion.
• ACE inhibitory effect of the protein hydrolysates prepared from commercially available nori product by pepsin–trypsin digestion
  Rie Morikawa; Keigo Toji; Yuya Kumagai; Hideki Kishimura
  European Food Research and Technology, Springer Science and Business Media LLC, 06 Oct. 2021, [Peer-reviewed]
  English, Scientific journal
• Characterization of ACE Inhibitory Peptides Prepared from Pyropia pseudolinearis Protein
  Yuya Kumagai; Keigo Toji; Satoshi Katsukura; Rie Morikawa; Toshiki Uji; Hajime Yasui; Takeshi Shimizu; Hideki Kishimura
  Marine Drugs, 19, 4, 200, 200, MDPI AG, 01 Apr. 2021, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal, More than 7000 red algae species have been classified. Although most of them are underused, they are a protein-rich marine resource. The hydrolysates of red algal proteins are good candidates for the inhibition of the angiotensin-I-converting enzyme (ACE). The ACE is one of the key factors for cardiovascular disease, and the inhibition of ACE activity is related to the prevention of high blood pressure. To better understand the relationship between the hydrolysates of red algal proteins and the inhibition of ACE activity, we attempted to identify novel ACE inhibitory peptides from Pyropia pseudolinearis. We prepared water soluble proteins (WSP) containing phycoerythrin, phycocyanin, allophycocyanin, and ribulose 1,5-bisphosphate carboxylase/oxygenase. In vitro analysis showed that the thermolysin hydrolysate of the WSP had high ACE inhibitory activity compared to that of WSP. We then identified 42 peptides in the hydrolysate by high-performance liquid chromatography and mass spectrometry. Among 42 peptides, 23 peptides were found in chloroplast proteins. We then synthesized the uncharacterized peptides ARY, YLR, and LRM and measured the ACE inhibitory activity. LRM showed a low IC50 value (0.15 μmol) compared to ARY and YLR (1.3 and 5.8 μmol). In silico analysis revealed that the LRM sequence was conserved in cpcA from Bangiales and Florideophyceae, indicating that the novel ACE inhibitory peptide LRM was highly conserved in red algae.
• Efficient Extraction and Antioxidant Capacity of Mycosporine-Like Amino Acids from Red Alga Dulse Palmaria palmata in Japan
  Yuki Nishida; Yuya Kumagai; Shunta Michiba; Hajime Yasui; Hideki Kishimura
  Marine Drugs, 18, 10, 502, 502, MDPI AG, 30 Sep. 2020, [Peer-reviewed]
  English, Scientific journal, Mycosporine-like amino acids (MAAs) are the ultraviolet (UV)-absorbable compounds, which are naturally produced by cyanobacteria and algae. Not only these algae but also marine organisms utilize MAAs to protect their DNA from UV-induced damage. On the other hand, the content of MAAs in algae was changed by the environmental condition and season. In addition to the UV-protected function, the antioxidant capacity of MAAs can apply to the cosmetic sunscreen materials and anti-cancer for human health. In this study, we developed the efficient extraction method of MAAs from red alga dulse in Usujiri (Hokkaido, Japan) and investigated the monthly variation. We also evaluated the antioxidant capacity. We employed the successive extraction method of water and then methanol extraction. Spectrophotometric and HPLC analyses revealed that the yield of MAAs by 6 h water extraction was the highest among the tested conditions, and the content of MAAs in the sample of February was the most (6.930 µmol g−1 dry weight) among the sample from January to May in 2019. Antioxidant capacity of MAAs such as crude MAAs, the purified palythine and porphyra-334 were determined by 2,2’-azinobis(3-ethylbenzothiazoline 6-sulfonic acid) (ABTS) radical scavenging and ferrous reducing power assays in various pH conditions, showing that the highest scavenging activity and reducing power were found at alkaline condition (pH 8.0).
• The Potential of Freshwater Fish Viscus from Silver Carp Hypophthalmichthys molitrix for Trypsin Source
  Kenichiro Abe; Chunhong Yuan; Yuya Kumagai; Hideki Kishimura
  Waste and Biomass Valorization, 11, 8, 3971, 3978, 01 Aug. 2020, [Peer-reviewed]
  English, Scientific journal
• Debittering of salmon (Salmo salar) frame protein hydrolysate using 2-butanol in combination with β-cyclodextrin: Impact on some physicochemical characteristics and antioxidant activities
  Avtar Singh; Anthony Temitope Idowu; Soottawat Benjakul; Hideki Kishimura; Rotimi E. Aluko; Yuya Kumagai
  Food Chemistry, 321, 126686, 126686, Elsevier BV, Aug. 2020, [Peer-reviewed]
  English, Scientific journal
• Identification of ACE inhibitory peptides from red alga Mazzaella japonica
  Yuya Kumagai; Yumi Kitade; Manami Kobayashi; Kei Watanabe; Hiroki Kurita; Hirohumi Takeda; Hajime Yasui; Hideki Kishimura
  European Food Research and Technology, Springer Science and Business Media LLC, 21 Jul. 2020, [Peer-reviewed], [Lead author]
  English, Scientific journal
• 紅藻ダルス由来キシロオリゴ糖のプレバイオティクスとしての可能性
  岸村 栄毅; 熊谷 祐也
  メディカル・サイエンス・ダイジェスト, 46, 7, 46, 47, Jul. 2020, [Invited]
  Japanese, Scientific journal
• In planta particle bombardment (iPB): A new method for plant transformation and genome editing.
  Ryozo Imai; Haruyasu Hamada; Yuelin Liu; Qianyan Linghu; Yuya Kumagai; Yozo Nagira; Ryuji Miki; Naoaki Taoka
  Plant Biotechnology (Tokyo, Japan), 37, 2, 171, 176, 25 Jun. 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Transformation is a key step in modern breeding technology that involves genome editing. The requirement for in vitro tissue culture and regeneration hampers application of this technology to commercially important varieties of many crop species. To overcome this problem, we developed a simple and reproducible in planta transformation method in wheat (Tritticum aestivum L.). Our in planta particle bombardment (iPB) method utilizes the shoot apical meristem (SAM) as a target tissue. The SAM contains a subepidermal cell layer termed L2, from which germ cells later develop during floral organogenesis. The iPB method can also be used for genome editing through transient CRISPR/Cas9 expression or direct delivery of the CRISPR/Cas9 ribonucleoprotein. In this review, we describe the iPB technology and provide an overview of its current and future applications in plant transformation and genome editing.
• In Silico Analysis of ACE Inhibitory Peptides from Chloroplast Proteins of Red Alga Grateloupia asiatica
  Kana Sumikawa; Kentaro Takei; Yuya Kumagai; Takeshi Shimizu; Hajime Yasui; Hideki Kishimura
  Marine Biotechnology, 22, 3, 391, 402, Springer Science and Business Media LLC, Jun. 2020, [Peer-reviewed]
  English, Scientific journal
• Identification of a Key Enzyme for the Hydrolysis of β-(1→3)-Xylosyl Linkage in Red Alga Dulse Xylooligosaccharide from Bifidobacterium Adolescentis
  Manami Kobayashi; Yuya Kumagai; Yohei Yamamoto; Hajime Yasui; Hideki Kishimura
  Marine Drugs, 18, 3, 174, 174, MDPI AG, 20 Mar. 2020, [Peer-reviewed]
  English, Scientific journal, Red alga dulse possesses a unique xylan, which is composed of a linear β-(1→3)/β-(1→4)-xylosyl linkage. We previously prepared characteristic xylooligosaccharide (DX3, (β-(1→3)-xylosyl-xylobiose)) from dulse. In this study, we evaluated the prebiotic effect of DX3 on enteric bacterium. Although DX3 was utilized by Bacteroides sp. and Bifidobacterium adolescentis, Bacteroides Ksp. grew slowly as compared with β-(1→4)-xylotriose (X3) but B. adolescentis grew similar to X3. Therefore, we aimed to find the key DX3 hydrolysis enzymes in B. adolescentis. From bioinformatics analysis, two enzymes from the glycoside hydrolase family 43 (BAD0423: subfamily 12 and BAD0428: subfamily 11) were selected and expressed in Escherichia coli. BAD0423 hydrolyzed β-(1→3)-xylosyl linkage in DX3 with the specific activity of 2988 mU/mg producing xylose (X1) and xylobiose (X2), and showed low activity on X2 and X3. BAD0428 showed high activity on X2 and X3 producing X1, and the activity of BAD0428 on DX3 was 1298 mU/mg producing X1. Cooperative hydrolysis of DX3 was found in the combination of BAD0423 and BAD0428 producing X1 as the main product. From enzymatic character, hydrolysis of X3 was completed by one enzyme BAD0428, whereas hydrolysis of DX3 needed more than two enzymes.
• Complete sequence of mitochondrial DNA of Gloiopeltis furcata (Postels and Ruprecht) J. Agardh
  Watanabe Kei; Kishimoto Takahiro; Kumagai Yuya; Shimizu Takeshi; Uji Toshiki; Yasui Hajime; Kishimura Hideki
  MITOCHONDRIAL DNA PART B-RESOURCES, 4, 2, 2543, 2544, 03 Jul. 2019, [Peer-reviewed]
• Complete sequence of mitochondrial DNA of red alga dulse Palmaria palmata (Linnaeus) Weber & Mohr in Japan
  Kumagai Yuya; Tsubouchi Ryota; Miyabe Yoshikatsu; Takeda Tomoyuki; Adachi Kohsuke; Yasui Hajime; Kishimura Hideki
  MITOCHONDRIAL DNA PART B-RESOURCES, 4, 2, 3177, 3178, 03 Jul. 2019, [Peer-reviewed], [Lead author]
  Scientific journal
• Enzymatic production of xylooligosaccharides from red alga dulse (Palmaria sp.) wasted in Japan
  Yamamoto Yohei; Kishimura Hideki; Kinoshita Yasunori; Saburi Wataru; Kumagai Yuya; Yasui Hajime; Ojima Takao
  PROCESS BIOCHEMISTRY, 82, 117, 122, Jul. 2019, [Peer-reviewed]
• A thermostable trypsin from freshwater fish Japanese dace (Tribolodon hakonensis): a comparison of the primary structures among fish trypsins.
  Kanno G; Klomklao S; Kumagai Y; Kishimura H
  Fish physiology and biochemistry, 45, 2, 561, 571, Apr. 2019, [Peer-reviewed]
• In Silico Analysis of Relationship between Proteins from Plastid Genome of Red Alga Palmaria sp. (Japan) and Angiotensin I Converting Enzyme Inhibitory Peptides.
  Kumagai Y; Miyabe Y; Takeda T; Adachi K; Yasui H; Kishimura H
  Marine drugs, 17, 3, 190, 190, MDPI AG, Mar. 2019, [Peer-reviewed], [Lead author]
  Scientific journal, Plastid proteins are one of the main components in red algae. In order to clarify the angiotensin I converting enzyme (ACE) inhibitory peptides from red alga Palmaria sp. (Japan), we determined the plastid genome sequence. The genome possesses 205 protein coding genes, which were classified as genetic systems, ribosomal proteins, photosystems, adenosine triphosphate (ATP) synthesis, metabolism, transport, or unknown. After comparing ACE inhibitory peptides between protein sequences and a database, photosystems (177 ACE inhibitory peptides) were found to be the major source of ACE inhibitory peptides (total of 751). Photosystems consist of phycobilisomes, photosystem I, photosystem II, cytochrome complex, and a redox system. Among them, photosystem I (53) and II (51) were the major source of ACE inhibitory peptides. We found that the amino acid sequence of apcE (14) in phycobilisomes, psaA (18) and psaB (13) in photosystem I, and psbB (11) and psbC (10) in photosystem II covered a majority of bioactive peptide sequences. These results are useful for evaluating the bioactive peptides from red algae.
• Antioxidant activity of proteins extracted from red alga dulse harvested in Japan
  Sato Naoto; Furuta Tomoe; Takeda Tomoyuki; Miyabe Yoshikatsu; Ura Kazuhiro; Takagi Yasuaki; Yasui Hajime; Kumagai Yuya; Kishimura Hideki
  JOURNAL OF FOOD BIOCHEMISTRY, 43, 2, e12709, e12709, Feb. 2019, [Peer-reviewed]
  Scientific journal
• Engineered dextranase from Streptococcus mutans enhances the production of longer isomaltooligosaccharides.
  Klahan P; Okuyama M; Jinnai K; Ma M; Kikuchi A; Kumagai Y; Tagami T; Kimura A
  Bioscience, biotechnology, and biochemistry, 82, 9, 1, 8, May 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Herein, we investigated enzymatic properties and reaction specificities of Streptococcus mutans dextranase, which hydrolyzes α-(1→6)-glucosidic linkages in dextran to produce isomaltooligosaccharides. Reaction specificities of wild-type dextranase and its mutant derivatives were examined using dextran and a series of enzymatically prepared p-nitrophenyl α-isomaltooligosaccharides. In experiments with 4-mg·mL-1 dextran, isomaltooligosaccharides with degrees of polymerization (DP) of 3 and 4 were present at the beginning of the reaction, and glucose and isomaltose were produced by the end of the reaction. Increased concentrations of the substrate dextran (40 mg·mL-1) yielded isomaltooligosaccharides with higher DP, and the mutations T558H, W279A/T563N, and W279F/T563N at the -3 and -4 subsites affected hydrolytic activities of the enzyme, likely reflecting decreases in substrate affinity at the -4 subsite. In particular, T558H increased the proportion of isomaltooligosaccharide with DP of 5 in hydrolysates following reactions with 4-mg·mL-1 dextran.Abbreviations CI: cycloisomaltooligosaccharide; CITase: CI glucanotransferase; CITase-Bc: CITase from Bacillus circulans T-3040; DP: degree of polymerization of glucose unit; GH: glycoside hydrolase family; GTF: glucansucrase; HPAEC-PAD: high performance anion-exchange chromatography-pulsed amperometric detection; IG: isomaltooligosaccharide; IGn: IG with DP of n (n, 2‒5); PNP: p-nitrophenol; PNP-Glc: p-nitrophenyl α-glucoside; PNP-IG: p-nitrophenyl isomaltooligosaccharide; PNP-IGn: PNP-IG with DP of n (n, 2‒6); SmDex: dextranase from Streptococcus mutans; SmDexTM: S. mutans ATCC25175 SmDex bearing Gln100‒Ile732.
• Polysaccharide-Degrading Enzymes From Marine Gastropods
  Takao Ojima; Mohammad M. Rahman; Yuya Kumagai; Ryuji Nishiyama; Joemark Narsico; Akira Inoue
  Methods in Enzymology, 605, 457, 497, Academic Press Inc., 01 Jan. 2018, [Peer-reviewed]
  English, In book
• A novel glycoside hydrolase family 97 enzyme: Bifunctional beta-L-arabinopyranosidase/alpha-galactosidase from Bacteroides thetaiotaomicron
  Asako Kikuchi; Masayuki Okuyama; Koji Kato; Shohei Osaki; Min Ma; Yuya Kumagai; Kana Matsunaga; Patcharapa Kiahan; Takayoshi Tagami; Min Yao; Atsuo Kimura
  BIOCHIMIE, 142, 41, 50, Nov. 2017, [Peer-reviewed]
  English, Scientific journal
• Effects of mutation of Asn694 in Aspergillus niger alpha-glucosidase on hydrolysis and transglucosylation
  Min Ma; Masayuki Okuyama; Megumi Sato; Takayoshi Tagami; Patcharapa Klahan; Yuya Kumagai; Haruhide Mori; Atsuo Kimura
  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101, 16, 6399, 6408, Aug. 2017, [Peer-reviewed]
  English, Scientific journal
• Substrate recognition of the catalytic alpha-subunit of glucosidase II from Schizosaccharomyces pombe
  Masayuki Okuyama; Masashi Miyamoto; Ichiro Matsuo; Shogo Iwamoto; Ryo Serizawa; Masanari Tanuma; Min Ma; Patcharapa Klahan; Yuya Kumagai; Takayoshi Tagami; Atsuo Kimura
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81, 8, 1503, 1511, 2017, [Peer-reviewed]
  English, Scientific journal
• Molecular insights into the mechanism of thermal stability of actinomycete mannanase
  Yuya Kumagai; Misugi Uraji; Kun Wan; Masayuki Okuyama; Atsuo Kimura; Tadashi Hatanaka
  FEBS LETTERS, 590, 17, 2862, 2869, Sep. 2016, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Heat treatment of curdlan enhances the enzymatic production of biologically active beta-(1,3)-glucan oligosaccharides
  Yuya Kumagai; Masayuki Okuyama; Atsuo Kimura
  CARBOHYDRATE POLYMERS, 146, 396, 401, Aug. 2016, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Megalo-type alpha-1,6-glucosaccharides induce production of tumor necrosis factor alpha in primary macrophages via toll-like receptor 4 signaling
  Ga-Hyun Joe; Midori Andoh; Aki Shinoki; Weeranuch Lang; Yuya Kumagai; Juri Sadahiro; Masayuki Okuyama; Atsuo Kimura; Hidehisa Shimizu; Hiroshi Hara; Satoshi Ishizuka
  BIOMEDICAL RESEARCH-TOKYO, 37, 3, 179, 186, 2016, [Peer-reviewed]
  English, Scientific journal
• The loop structure of Actinomycete glycoside hydrolase family 5 mannanases governs substrate recognition
  Yuya Kumagai; Keitaro Yamashita; Takayoshi Tagami; Misugi Uraji; Kun Wan; Masayuki Okuyama; Min Yao; Atsuo Kimura; Tadashi Hatanaka
  FEBS JOURNAL, 282, 20, 4001, 4014, Oct. 2015, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Biochemical properties and substrate recognition mechanism of GH31 alpha-glucosidase from Bacillus sp AHU 2001 with broad substrate specificity
  Wataru Saburi; Masayuki Okuyama; Yuya Kumagai; Atsuo Kimura; Haruhide Mori
  BIOCHIMIE, 108, 140, 148, Jan. 2015, [Peer-reviewed]
  English, Scientific journal
• Different molecular complexity of linear-isomaltomegalosaccharides and beta-cyclodextrin on enhancing solubility of azo dye ethyl red: Towards dye biodegradation
  Weeranuch Lang; Yuya Kumagai; Juri Sadahiro; Janjira Maneesan; Masayuki Okuyama; Haruhide Mori; Nobuo Sakairi; Atsuo Kimura
  BIORESOURCE TECHNOLOGY, 169, 518, 524, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• A laminaribiose-hydrolyzing enzyme, AkLab, from the common sea hare Aplysia kurodai and its transglycosylation activity
  Yuya Kumagai; Takuya Satoh; Akira Inoue; Takao Ojima
  COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 167, 1, 7, Jan. 2014, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Enzymatic production of ferulic acid from defatted rice bran by using a combination of bacterial enzymes
  Misugi Uraji; Masayo Kimura; Yosikazu Inoue; Kayoko Kawakami; Yuya Kumagai; Koichi Harazono; Tadashi Hatanaka
  Applied Biochemistry and Biotechnology, 171, 5, 1085, 1093, Nov. 2013, [Peer-reviewed]
  English, Scientific journal
• Effect of the binding of bivalent ion to the calcium-binding site responsible for the thermal stability of actinomycete mannanase: Potential use in production of functional mannooligosaccharides
  Yuya Kumagai; Kayoko Kawakami; Misugi Uraji; Tadashi Hatanaka
  JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 94, 63, 68, Oct. 2013, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai
  Yuya Kumagai; Takuya Satoh; Akira Inoue; Takao Ojima
  Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 164, 2, 80, 88, 2, Feb. 2013, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Binding of bivalent ions to actinomycete mannanase is accompanied by conformational change and is a key factor in its thermal stability
  Yuya Kumagai; Kayoko Kawakami; Misugi Uraji; Tadashi Hatanaka
  Biochimica et Biophysica Acta - Proteins and Proteomics, 1834, 1, 301, 307, 1, Jan. 2013, [Peer-reviewed], [Lead author]
  English, Scientific journal
• A novel mechanism for the promotion of quercetin glycoside absorption by megalo alpha-1,6-glucosaccharide in the rat small intestine
  Aki Shinoki; Weeranuch Lang; Charin Thawornkuno; Hee-Kwon Kang; Yuya Kumagai; Masayuki Okuyama; Haruhide Mori; Atsuo Kimura; Satoshi Ishizuka; Hiroshi Hara
  FOOD CHEMISTRY, 136, 2, 293, 296, Jan. 2013, [Peer-reviewed]
  English, Scientific journal
• The structural analysis and the role of calcium binding site for thermal stability in mannanase
  Yuya Kumagai; Kayoko Kawakami; Takafumi Mukaihara; Masayo Kimura; Tadashi Hatanaka
  BIOCHIMIE, 94, 12, 2783, 2790, Dec. 2012, [Peer-reviewed], [Lead author]
  English, Scientific journal
• The Delay in the Development of Experimental Colitis from Isomaltosyloligosaccharides in Rats Is Dependent on the Degree of Polymerization
  Hitoshi Iwaya; Jae-Sung Lee; Shinya Yamagishi; Aki Shinoki; Weeranuch Lang; Charin Thawornkuno; Hee-Kwon Kang; Yuya Kumagai; Shiho Suzuki; Shinichi Kitamura; Hiroshi Hara; Masayuki Okuyama; Haruhide Mori; Atsuo Kimura; Satoshi Ishizuka
  PLOS ONE, 7, 11, e50658, Nov. 2012, [Peer-reviewed]
  English, Scientific journal
• Production of dipeptidyl peptidase IV inhibitory peptides from defatted rice bran
  Tadashi Hatanaka; Yosikazu Inoue; Jiro Arima; Yuya Kumagai; Hirokazu Usuki; Kayoko Kawakami; Masayo Kimura; Takafumi Mukaihara
  FOOD CHEMISTRY, 134, 2, 797, 802, Sep. 2012, [Peer-reviewed]
  English, Scientific journal
• Preparation of hemicellulolic oligosaccharides from Chamaecyparis obtuse (Hinoki) slurry using commercial enzymes
  Yuya Kumagai; Hirokazu Usuki; Yukihiro Yamamoto; Akihiro Yamasato; Takafumi Mukaihara; Tadashi Hatanaka
  Frontiers of Chemical Science and Engineering, 6, 2, 224, 231, Jun. 2012, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Characterization of calcium ion sensitive region for beta-Mannanase from Streptomyces thermolilacinus
  Yuya Kumagai; Hirokazu Usuki; Yukihiro Yamamoto; Akihiro Yamasato; Jiro Arima; Takafumi Mukaihara; Tadashi Hatanaka
  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1814, 9, 1127, 1133, Sep. 2011, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Synthesis of prolyl-hydroxyproline using prolyl aminopeptidase from Streptomyces aureofaciens TH-3
  Yukihiro Yamamoto; Hirokazu Usuki; Yuya Kumagai; Takafumi Mukaihara; Akihiro Yamasato; Tadashi Hatanaka
  PROCESS BIOCHEMISTRY, 46, 8, 1560, 1564, Aug. 2011, [Peer-reviewed]
  English, Scientific journal
• Extracellular Production and Characterization of Streptomyces X-prolyl Dipeptidyl Aminopeptidase
  Tadashi Hatanaka; Akihiro Yamasato; Jiro Arima; Hirokazu Usuki; Yukihiro Yamamoto; Yuya Kumagai
  APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 164, 4, 475, 486, Jun. 2011, [Peer-reviewed]
  English, Scientific journal
• Extracellular Production and Characterization of Two Streptomyces L-Asparaginases
  Tadashi Hatanaka; Hirokazu Usuki; Jiro Arima; Yoshiko Uesugi; Yukihiro Yamamoto; Yuya Kumagai; Akihiro Yamasato; Takafumi Mukaihara
  APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 163, 7, 836, 844, Apr. 2011, [Peer-reviewed]
  English, Scientific journal
• MS/MS fragmentation-guided search of TMG-chitooligomycins and their structure-activity relationship in specific beta-N-acetylglucosaminidase inhibition
  Hirokazu Usuki; Yukihiro Yamamoto; Yuya Kumagai; Teruhiko Nitoda; Hiroshi Kanzaki; Tadashi Hatanaka
  ORGANIC & BIOMOLECULAR CHEMISTRY, 9, 8, 2943, 2951, 2011, [Peer-reviewed]
  English, Scientific journal
• Isolation and characterization of two types of beta-1,3-glucanases from the common sea hare Aplysia kurodai
  Yuya Kumagai; Takao Ojima
  COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 155, 2, 138, 144, Feb. 2010, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Enzymatic properties and the primary structure of a beta-1,3-glucanase from the digestive fluid of the Pacific abalone Haliotis discus hannai
  Yuya Kumagai; Takao Ojima
  COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 154, 1, 113, 120, Sep. 2009, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Comparative study on general properties of alginate lyases from some marine gastropod mollusks
  Mami Hata; Yuya Kumagai; Mohammad Matiur Rahman; Satoru Chiba; Hiroyuki Tanaka; Akira Inoue; Takao Ojima
  FISHERIES SCIENCE, 75, 3, 755, 763, May 2009, [Peer-reviewed]
  English, Scientific journal
• Preparation of beta-1,3-glucanase from scallop mid-gut gland drips and its use for production of novel heterooligosaccharides
  Yuya Kumagai; Akira Inoue; Hiroyuki Tanaka; Takao Ojima
  FISHERIES SCIENCE, 74, 5, 1127, 1136, Oct. 2008, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus
  Yukiko Nishida; Ken-ichi Suzuki; Yuya Kumagai; Hiroyuki Tanaka; Akira Inoue; Takao Ojima
  BIOCHIMIE, 89, 8, 1002, 1011, Aug. 2007, [Peer-reviewed]
  English, Scientific journal

• 海洋深層水を活用した海藻スプラウトの陸上養殖と利用適性に関する研究
  木下康宣; 木村和世; 川越力; 高野智宏; 熊谷祐也; 岸村栄毅; 宇治利樹; 水田浩之, 北海道立工業技術センター研究成果発表会要旨集(Web), 2021, 2021
• デキストランデキストリナーゼの多糖合成に関与するアミノ酸の同定
  佐々木優希; 熊谷祐也; 貞廣樹里; LANG Weeranuch; 田上貴祥; 奥山正幸; 木村淳夫, 日本農芸化学会大会講演要旨集(Web), 2018, ROMBUNNO.2A26p03 (WEB ONLY), 05 Mar. 2018
  Japanese
• Bacillus sp.AHU2001由来GH31α‐グルコシダーゼの生化学的諸性質と基質認識に重要な構造因子の解析
  佐分利亘; 奥山正幸; 熊谷祐也; 木村淳夫; 森春英, 日本農芸化学会大会講演要旨集(Web), 2015, 3E32P02 (WEB ONLY), 05 Mar. 2015
  Japanese
• Bp-6 Amphiphilic function of linear-isomaltomegalosaccharides (L-IMS) on ethyl red (ER) solubility
  Lang Weeranuch; Kumagai Yuya; Sadahiro Juri; Okuyama Masayuki; Mori Haruhide; Sakairi Nobuo; Kimura Atsuo, Bulletin of applied glycoscience, 4, 3, B42, 20 Aug. 2014
  The Japanese Society of Applied Glycoscience, English
• Ba-3 Dextran dextrinaseのPhe602の生成物特異性に関する影響(その他の糖質関連酵素,一般講演,日本応用糖質科学会平成26年度大会(第63回))
  坂谷 敦; 熊谷 祐也; 貞廣 樹里; Weeranuch Lang; 奥山 正幸; 森 春英; 木村 淳夫, 応用糖質科学：日本応用糖質科学会誌, 4, 3, B39, 2014
  一般社団法人 日本応用糖質科学会, Japanese
• Da-6 放線菌マンナナーゼの基質特異性に関するループ7の解析(ヘミセルラーゼ,一般講演,日本応用糖質科学会平成26年度大会(第63回))
  熊谷 祐也; 裏地 美杉; 奥山 正幸; 木村 淳夫; 畑中 唯史, 応用糖質科学：日本応用糖質科学会誌, 4, 3, B53, 2014
  一般社団法人 日本応用糖質科学会, Japanese
• 2P-052 Insight into the substrate specificity of galactosylmannooligosaccharide by actinomycete mannanase
  Kumagai Yuya; Uraji Misugi; Okuyama Masayuki; Kimura Atsuo; Hatanaka Tadashi, 日本生物工学会大会講演要旨集, 66, 119, 119, 2014
  日本生物工学会, Japanese
• Da-10 Gluconobacter oxydans由来dextran dextrinaseによる直鎖イソマルトメガロ糖の酵素合成(糖質の合成・生産,一般講演,日本応用糖質科学会平成25年度大会(第62回))
  熊谷 祐也; Lang Weeranuch; 貞廣 樹里; 奥山 正幸; 森 春英; 木村 淳夫, 応用糖質科学：日本応用糖質科学会誌, 3, 3, B46, 2013
  一般社団法人 日本応用糖質科学会, Japanese
• 1Ep10 Inhibitory Action of Xaa-Pro Dipeptides toward Human Dipeptidyl Peptidase IV
  Hatanaka Tadashi; Kumagai Yuya; Usuki Hirokazu; Kawakami Kayoko; Mukaihara Takafumi, 日本生物工学会大会講演要旨集, 63, 41, 41, 2011
  日本生物工学会, Japanese
• Comparative study on general properties of alginate lyases from some marine gastropod mollusks
  RAHMAN Mohammad Matiur, 日本水産學會誌 = Bulletin of the Japanese Society of Scientific Fisheries, 76, 4, 580, 580, 15 Jul. 2010
  Japanese
• エゾアワビ肝膵臓におけるアルギン酸代謝に関する研究
  木本雄太; 熊谷祐也; 澤辺智雄; 田中啓之; 井上晶; 尾島孝男, 日本水産学会大会講演要旨集, 2010, 2010
• 2P-1071 Clarification of the reaction mechanism of aminolysin and its application into the effective synthesis of cyclic dipeptides
  Usuki Hirokazu; Yamamoto Yukihiro; Yamasato Akihiro; Kumagai Yuya; Mukaihara Takafumi; Hatanaka Tadashi, 日本生物工学会大会講演要旨集, 22, 26, 26, 2010
  日本生物工学会, Japanese
• 3P-1054 Analysis of Ca^<2+> dependent domain of mannanase from Streptomyces sp.
  KUMAGAI Yuya; USUKI Hirokazu; YAMAMOTO Yukihiro; YAMASATO Akihiro; MUKAIHARA Takafumi; HATANAKA Tadashi, 日本生物工学会大会講演要旨集, 22, 71, 71, 2010
  日本生物工学会, Japanese
• 3P-1158 Collagen degradation by combination of Streptomyces aminopeptidases
  HATANAKA Tadashi; USUKI Hirokazu; YAMAMOTO Yukihiro; KUMAGAI Yuya; YAMASATO Akihiro; MUKAIHARA Takafumi, 日本生物工学会大会講演要旨集, 22, 97, 97, 2010
  日本生物工学会, Japanese
• 海洋資源-7つの不思議と11の挑戦-, 大塚耕司編, 海事プレス社, 2007年, A5版, 196頁, 1,429円
  熊谷 祐也; 北大院, 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries, 74, 2, 15 Mar. 2008
  Japanese

• Advances in Oligosaccharides and Polysaccharide Modifications in Marine Bioresources
  Yuya Kumagai; Hideki Kishimura; Benwei Zhu
  Mdpi AG, 09 Aug. 2023, 3036583645, 176, [Editor]
• Marine_Bioactive_PeptidesStructure_Function_and_Application
  A.M.M.Martin; Y.Miyabe; T.Shimizu; W.Matsui; Y.Kumagai; H.Kishimura, pp.63-77
  MDPI, Jul. 2023, 9783036582603, [Contributor]
• Mycosporine-Like Amino Acids from Marine Resource
  Y. Nishida; Y. Kumagai; S. Michiba; H. Yasui; H. Kishimura, pp. 39-56
  Marine Drugs, Multidiciplinary Digital Publishing Institute（MDPI）, Jan. 2021, [Joint work]

• 紅藻ダルス由来DPP-IV阻害ペプチド に関する研究
  熊谷祐也; Martin Mune Mune Alain; 畑中唯史
  令和8年度日本水産学会春季大会, 29 Mar. 2026, Japanese, Oral presentation
  26 Mar. 2026 - 29 Mar. 2026
• 紅藻ウミゾウメン由来DPP-4阻害 ペプチドの探索
  熊谷 祐也; 太田 龍成; 畑中 唯史; ムネムネ マーティン
  日本農芸化学会 2026年度大会, 11 Mar. 2026, Japanese, Oral presentation
  09 Mar. 2026 - 12 Mar. 2026
• ブルーカーボンに資する海藻を活用したヘルスケア製品の開発
  熊谷祐也
  これからの水産ビジネス～大学は何ができるのか？～, 01 Dec. 2025, Japanese, Invited oral presentation
  01 Dec. 2025 - 01 Dec. 2025, [Invited]
• フコイダンによる血液凝固の抑制と促進～魚類と哺乳類で異なる働き～
  長澤和貴; 青木建斗; 曽我英利; 辻井豪佑; 太田龍成; 岸村 栄毅; 熊谷 祐也
  HAKODATEアカデミックリンク2025, 09 Nov. 2025, Japanese, Oral presentation
  09 Nov. 2025 - 09 Nov. 2025
• Upcycled Food and ONE Health Focusing on Seaweed in Hokkaido
  YUYA KUMAGAI
  2025 KFN International Symposium and Annual Meeting, 31 Oct. 2025, English, Invited oral presentation
  29 Oct. 2025 - 31 Oct. 2025, [Invited]
• 亜臨界水処理マコンブ含まれるグルコシルトランスフェラーゼ阻害物質に関する研究
  熊谷祐也; 畑中唯史; 阿座上弘行
  令和7年度日本水産学会秋季大会, 25 Sep. 2025, Japanese, Oral presentation
  24 Sep. 2025 - 27 Sep. 2025
• 亜臨界水処理を施したコンブの グルコシルトランスフェラーゼ阻害物質の探索
  畑中 唯史; 楊 霊麗; 逸見 健司; 木下 颯; 阿座上 弘行; 熊谷祐也
  学会創立100周年記念 日本農芸化学会 2025年度 中四国支部 第71回講演, 14 Jun. 2025, Japanese, Oral presentation
  14 Jun. 2025 - 14 Jun. 2025
• 紅藻スサビノリのフィコビリソームに関する研究
  熊谷祐也; 宇治利樹
  令和7年度（２０２５年度）日本水産学会春季大会, 27 Mar. 2025, Japanese, Oral presentation
  26 Mar. 2025 - 29 Mar. 2025
• 紅藻スサビノリのフィコビリソームの光応答の解析
  熊谷祐也; 宇治利樹
  日本農芸化学会 2025年度大会, 07 Mar. 2025, Japanese, Poster presentation
  04 Mar. 2025 - 08 Mar. 2025
• Biochemical analysis of novel Xylanase belonging to Glycosyl Hydrolases 141Family
  Sajeevan Shilja; Kumagai Yuya; Kishimura Hideki
  令和 6 年度日本水産学会北海道支部大会, 11 Jan. 2025, Japanese, Oral presentation
  11 Jan. 2025 - 11 Jan. 2025
• 紅藻ウミゾウメンの葉緑体ゲノムにコードされるタンパク質由来の DPP-IV 阻害ペプチドに関する in silico および in vitro 研究
  太田龍成; Martin Alainn; Mune Mune; 熊谷祐也; 岸村栄毅
  令和 6 年度日本水産学会北海道支部大会, 11 Jan. 2025, Japanese, Oral presentation
  11 Jan. 2025 - 11 Jan. 2025
• 道南の 2 地点で採取されたダルス由来マイコスポリン様アミノ酸の含有量変動に関する研究
  瀧澤巧季; 熊谷祐也; 岸村栄毅
  令和 6 年度日本水産学会北海道支部大会, 11 Jan. 2025, Japanese, Oral presentation
  11 Jan. 2025 - 11 Jan. 2025
• フコイダンの抗酸化活性は硫酸基に由来するのか
  馬場惇平; 熊谷裕也; 岸村栄毅
  令和 6 年度日本水産学会北海道支部大会, 11 Jan. 2025, Japanese, Oral presentation
  11 Jan. 2025 - 11 Jan. 2025
• Fucoidan from Saccharina japonica: Purification by molecular weight
  Jumpei Baba; Yuya Kumagai; Hideki Kishimura
  14th Joint International Symposium on Food Science and Technology, 21 Nov. 2024, English, Poster presentation
  20 Nov. 2024 - 21 Nov. 2024
• A comparison of mycosporine-like amino acids (MAAs) from red alga dulse (Devaleraea inkyuleei) harvested from two locations
  Koki Takizawa; Soichiro Hakura; Yuya Kumagai; Hideki Kishimura
  14th Joint International Symposium on Food Science and Technology, 21 Nov. 2024, English, Poster presentation
  20 Nov. 2024 - 21 Nov. 2024
• Sustainable Livestock Production Enabled by Fisheries Waste
  Gosuke TSUJII; Yuya KUMAGAI; Hideki KISHIMURA
  14th Joint International Symposium on Food Science and Technology, 21 Nov. 2024, English, Oral presentation
  20 Nov. 2024 - 21 Nov. 2024
• In silico and in vitro investigation on DPP-IV inhibitory peptides encrypted in Nemalion vermiculare chloroplast genome
  Ryusei Ota; Martin Alain Mune Mune; Yuya Kumagai; Hideki Kishimura
  14th Joint International Symposium on Food Science and Technology, 20 Nov. 2024, English, Poster presentation
  20 Nov. 2024 - 21 Nov. 2024
• Characterization of novel xylanase belonging to glycoside hydrolase family 141
  Sajeevan Shilja; Kishimura Hideki; Kumagai Yuya
  14th Joint International Symposium on Food Science and Technology, 20 Nov. 2024, English, Oral presentation
  20 Nov. 2024 - 21 Nov. 2024
• 低利用資源「海のパセリ」のここがすごい！ ～糖尿病予防に寄与する可能性を明らかに～
  太田 龍成; 馬場 惇平; 石川勇斗; 岸村栄毅; 熊谷祐也
  HAKODATEアカデミックリンク2024, 11 Nov. 2024, Japanese, Oral presentation
  11 Nov. 2024 - 11 Nov. 2024
• ダルスの飲水投与が活動リズムおよび認知機能に及ぼす影響
  宮本 舜佑; 篠塚 まりな; 谷口 楓果; 森田 竜未; 池上 啓介; 熊谷 祐也; 岸村 栄毅; 中熊 大英; 池口 主弥; 安尾 しのぶ
  第11回時間栄養学会, 24 Aug. 2024, Japanese, Oral presentation
  23 Aug. 2024 - 24 Aug. 2024
• 真昆布から中間素材の調製とその特性
  馬場惇平; 菅原一真; 熊谷祐也; 岸村栄毅
  海藻活用研究会令和6年度定期総会シンポジウム, 28 Jun. 2024, Japanese, Invited oral presentation
  [Invited]
• Mycosporine-like amino acids from red alga dulse (Devaleraea inkyukeei) harvested from two location in southern Hokkaido
  Koki Takizawa; Soichiro Hakura; Yuya Kumagai; Hideki Kishimura
  KoSFoST 2024 (International Symposium and Annual Meeting), 04 Jun. 2024, English, Poster presentation
  03 Jun. 2024 - 05 Jun. 2024
• Fucoidan from Kombu Saccharine japonica: separation by molecular weight
  Jumpei Baba; Yuya Kumagai; Hideki Kishimura
  KoSFoST 2024 (International Symposium and Annual Meeting), 03 Jun. 2024, English, Poster presentation
  03 Jun. 2024 - 05 Jun. 2024
• Function of underusage red alga dulse in Kombu cultivation rope
  Yuya Kumagai
  KoSFoST 2024 (International Symposium and Annual Meeting), 03 Jun. 2024, English, Invited oral presentation
  03 Jun. 2024 - 05 Jun. 2024, [Invited]
• 函館産マコンブの多糖成分の機能性について
  熊谷祐也; 岸村栄毅
  海藻活用研究会 令和6年シンポジウム, 22 Feb. 2024, Japanese, Invited oral presentation
  [Invited]
• 4種紅藻由来マイコスポリン様アミノ酸に関する研究
  山本竜矢; 熊谷祐也; 岸村栄毅
  令和5年度 日本水産学会 北海道支部大会, 20 Jan. 2024, Japanese, Oral presentation
• 函館産マコンブ Saccharina japonica 由来多糖類の調製と その収量，組成及び抗酸化作用に関する研究
  小島大樹; 今武洋人; 田畑大伍; 岸村栄毅; 熊谷祐也
  令和5年度 日本水産学会 北海道支部大会, 20 Jan. 2024, Japanese, Oral presentation
• 4種紅藻由来マイコスポリン様アミノ酸の 含有量及び組成の月別変動と抗酸化活性
  山本竜矢; 熊谷祐也; 岸村栄毅
  2023年度 日本農芸化学会北海道支部 第２回学術講演会, 09 Dec. 2023, Japanese, Oral presentation
  09 Dec. 2023 - 10 Dec. 2023
• 函館産マコンブ由来フコイダンの月別変動
  馬場惇平; 熊谷祐也; 岸村栄毅
  2023年度 日本農芸化学会北海道支部 第２回学術講演会, 09 Dec. 2023, Japanese, Oral presentation
  09 Dec. 2023 - 10 Dec. 2023
• Fucoidan from Kombu Saccharina japonica: Monthly variation and structure analysis
  Jumpei Baba; Yuya Kumagai; Hideki Kishimura
  13th Joint International Symposium on Food Science and Technology Among NUS, TUMSAT, HU, and KU, 21 Nov. 2023, English, Oral presentation
  20 Nov. 2023 - 22 Nov. 2023
• Study on health functional components of seaweed
  Yuya Kumagai
  13th Joint International Symposium on Food Science and Technology Among NUS, TUMSAT, HU, and KU, 21 Nov. 2023, English, Keynote oral presentation
  [Invited]
• 天然日焼け止め成分 「MAAs」含有量の変動を追え！
  中目 凛; 森田理央; 瀧澤巧季; 山本竜矢; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2023, 03 Nov. 2023, Japanese, Oral presentation
• 劇場版 KOMBU×FAMILY CODE：Brown
  濱崎海瑠; 馬場惇平; 張昕然; 濱崎海瑠; 田畑太伍; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2023, 03 Nov. 2023, Japanese, Oral presentation
• 水産廃棄物が可能にするサステイナブルな家畜生産への道 ：水産・獣医連携の秘める大きな可能性
  辻井豪佑; 熊谷祐也; 中尾亮
  第6回 北大・部局横断シンポジウム, 11 Oct. 2023, Japanese, Oral presentation
• 函館産マコンブ由来フコイダンの月別変動 及び構造特性について
  馬場惇平; 熊谷裕也; 岸村栄毅
  第6回 北大・部局横断シンポジウム, 11 Oct. 2023, Japanese, Poster presentation
• 真昆布からの中間素材の物性・機能性評価
  熊谷祐也; 岸村栄毅
  海藻活用研究会 令和5年度 総会, 15 Jun. 2023, Japanese
  15 Jun. 2023 - 15 Jun. 2023, [Invited]
• 紅藻ウミゾウメンの葉緑体DNAにコードされるタンパク質に由来するDPP-IV阻害ペプチドのin silico解析
  Martin Alain Mune Mune; 陳 瑞郷; 熊谷祐也; 岸村栄毅
  第23回マリンバイオテクノロジー学会大会, 28 May 2023, Japanese, Oral presentation
  27 May 2023 - 28 May 2023
• Polysaccharides from brown algae: relationship between structure and function
  Yuya Kumagai; Hiroki Kojima; Jumpei Baba; Hideki Kishimura
  East Asia Fisheries Technologists Association (EAFTA) 2023, 19 May 2023, English, Invited oral presentation
  17 May 2023 - 20 May 2023, [Invited]
• 函館産褐藻由来の多糖成分について
  岸村栄毅; 馬場惇平; 熊谷祐也
  海藻活用研究会 令和４年度定期シンポジウム, 08 Mar. 2023, Japanese, Nominated symposium
  08 Mar. 2023 - 08 Mar. 2023, [Invited]
• ビフィズス菌の増殖に適した 紅藻ダルス由来キシロオリゴ糖の組成
  吉田光里; 熊谷祐也; 岸村栄毅
  日本水産学会 北海道支部大会, 26 Nov. 2022, Japanese, Oral presentation
  26 Nov. 2022 - 26 Nov. 2022
• 函館産褐藻由来フコイダンの比較
  馬場惇平; 熊谷祐也; 岸村栄毅
  日本水産学会 北海道支部大会, 26 Nov. 2022, Japanese, Oral presentation
  26 Nov. 2022 - 26 Nov. 2022
• 紅藻ダルス由来のマイコスポリン様アミノ酸の 月別変動および効率的な抽出方法の検討
  山本竜矢; 熊谷祐也; 岸村栄毅
  令和4年度 日本農芸化学会 北海道・東北合同支部会, 21 Sep. 2022, Japanese
  20 Sep. 2022 - 21 Sep. 2022
• 紅藻ダルス由来タンパク質のDPPⅣ阻害作用に関する研究
  松井 亘; 熊谷祐也; 岸村栄毅
  令和4年度 日本農芸化学会 北海道・東北合同支部会, 21 Sep. 2022, Japanese
  20 Sep. 2022 - 21 Sep. 2022
• 紅藻ダルス由来キシランから亜臨界水を用いたオリゴ糖の調製に関する研究
  辰巳幸彦; 熊谷祐也; Byung-Soo Chun; 岸村栄毅
  令和4年度 日本農芸化学会 北海道・東北合同支部会, 21 Sep. 2022, Japanese, Oral presentation
  20 Sep. 2022 - 21 Sep. 2022
• 塩ストレスによる紅藻類の食味改善物質の同定と機構に関する研究
  熊谷 祐也
  公益財団法人ソルトサイエンス第34回助成研究発表会, 26 Jul. 2022, Japanese, Oral presentation
  [Invited]
• 紅藻ツルシラモ由来フィコエリスロビリンの生合成に関わる酵素に関する研究
  宮部好克; 熊谷祐也; 清水健志; 松井 亘; 佐藤諒介; 岸村栄毅
  第22回マリンバイオテクノロジー学会大会, 28 May 2022, Japanese, Poster presentation
  28 May 2022 - 29 May 2022
• 紅藻ツルシラモ由来フィコエリスリン・ガンマ鎖の構造特性
  熊谷祐也; 宮部好克; 清水健志; 佐藤諒介; 岸村栄毅
  令和４年度日本水産学会春季大会, 27 Mar. 2022, Japanese, Oral presentation
  26 Mar. 2022 - 29 Mar. 2022
• 紅藻類のマイコスポリン様アミノ酸の可能性
  岸村栄毅; 熊谷祐也; 木下康宣; 安井 肇; 川越 力
  海藻活用研究会定期シンポジウム 〜ウィズコロナの海藻活用〜, 25 Feb. 2022, Japanese, Nominated symposium
  25 Feb. 2022 - 25 Feb. 2022
• ダルスが腸に届くまで
  辰巳幸彦; 松本晃帆; 吉田光里; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2021, 23 Nov. 2021, Japanese, Poster presentation
• 海藻のヌメヌメ成分「フコイダン」の最大Maxパワー力を生かすために
  伊藤大翔; 松井 亘; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミクリンク2021, 23 Nov. 2021, Japanese, Poster presentation
• 季節変動から紐解くダルス「MAAs」産業利用への試み
  山本竜矢; 前田侑也; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2021, 23 Nov. 2021, Japanese, Oral presentation
• 紅藻ツルシラモ由来フィコエリスリン の構造・機能特性
  前田俊介; 熊谷侑貴; 宮部好克; 佐藤諒介; 熊谷祐也; 岸村栄毅
  令和3年度 日本水産学会秋季大会, 14 Sep. 2021, Japanese, Oral presentation
  13 Sep. 2021 - 16 Sep. 2021
• 紅藻ダルス由来β-(1→3)/β-(1→4)キシランの 腸内細菌増殖作用の検討
  吉田光里; 熊谷祐也; 岸村栄毅
  令和3年度 日本水産学会秋季大会, 14 Sep. 2021, Japanese, Oral presentation
  13 Sep. 2021 - 16 Sep. 2021
• Vibrio由来glycoside hydrolase family 17 β-1,3-glucanaseの機能解析
  熊谷祐也; 岸村栄毅
  令和3年度 日本水産学会秋季大会, 14 Sep. 2021, Japanese, Oral presentation
  13 Sep. 2021 - 16 Sep. 2021
• 紅藻ダルス小形葉体の 栄養特性に関する研究
  木下康宣; 鳥海 滋; 川越 力; 木村和世; 熊谷祐也; 岸村栄毅
  日本応用藻類学会 第19回大会, 04 Sep. 2021, Japanese, Oral presentation
  04 Sep. 2021 - 04 Sep. 2021
• 紅藻ウミゾウメン葉緑体DNAにコードされるタンパク質由来ACE阻害ペプチドのin silico解析
  陳 瑞卿; 丁 佳琦; 水田浩之; 熊谷祐也; 岸村栄毅
  2021年度 日本農芸化学会北海道支部 第1 回学術講演会, 24 Jul. 2021, Japanese, Oral presentation
  24 Jul. 2021 - 24 Jul. 2021
• 海洋深層水を活用した海藻スプラウトの 陸上養殖と利用適性に関する研究
  木下康宣; 木村和世; 川越 力; 高野智宏; 熊谷祐也; 岸村栄毅; 宇治利樹; 水田浩之
  2021年度 北海道立坑業技術センター研究成果発表会, 09 Jun. 2021, Japanese, Oral presentation
  09 Jun. 2021 - 09 Jun. 2021
• Bifidobacterium adolescentisによるβ-(1→3)-キシロシル-キシロビオース分解機構の解明
  小林真奈美; 熊谷祐也; 岸村栄毅
  R2年度 日本応用糖質科学会 北海道支部 支部賞授賞式・受賞公演およびシンポジウム, 28 Jan. 2021, Japanese, Oral presentation
  28 Jan. 2021 - 28 Jan. 2021
• 紅藻ダルス由来β-(1→3)/β-(1→4)-キシロオリゴ糖調製法の開発およびエンド型キシラナーゼの基質特異性
  藤井勇樹; 熊谷祐也; 岸村栄毅; 畑中 唯史
  R2年度 日本応用糖質科学会 北海道支部 支部賞授賞式・受賞公演およびシンポジウム, 28 Jan. 2021, Japanese, Oral presentation
  28 Jan. 2021 - 28 Jan. 2021
• 人類を紫外線から守る海藻？！ 函館産紅藻に秘めたるMAAsのポテンシャル
  杉山佳奈海; 西田有輝; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2020, 28 Nov. 2020, Japanese, Public discourse
  28 Nov. 2020 - 20 Dec. 2020
• 絶対正義オリゴ糖～もっと海藻は輝ける～
  吉田里光; 小林真奈美; 栗田大輝; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2020, 28 Nov. 2020, Japanese, Public discourse
  28 Nov. 2020 - 20 Dec. 2020
• 天然＆陸上栽培された紅藻成分の相違とは？
  大井一真; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2020, 28 Nov. 2020, Japanese, Public discourse
  28 Nov. 2020 - 20 Dec. 2020
• 放線菌 Streptomyces thermogriseus エンド型キシラナーゼを用いた紅藻ダルス β(1-3/1-4)キシロオリゴ糖調製法の開発
  藤井 勇樹; 熊谷 祐也; 岸村 栄毅; 畑中 唯史
  2020年度 日本農芸化学会北海道支部／日本栄養・食糧学会北海道支部 合同学術講演会, 12 Dec. 2020, Japanese, Oral presentation
  12 Dec. 2020 - 13 Dec. 2020
• 岩のり製品由来タンパク質から調製したペプチドの ACE 阻害作用
  森河理絵; 熊谷祐也; 岸村栄毅
  令和 2 年度日本水産学会 北海道支部大会, 05 Dec. 2020, Japanese
  05 Dec. 2020 - 05 Dec. 2020
• 紅藻ダルス由来キシランの簡易調製法の開発とその酵素分解物による Bifidobacterium の増殖
  栗田大輝; 熊谷祐也; 安井 肇; 岸村栄毅
  令和 2 年度日本水産学会 北海道支部大会, 05 Dec. 2020, Japanese, Oral presentation
  05 Dec. 2020 - 05 Dec. 2020
• Efficient Extraction and Monthly Variation of Mycosporine-like Amino Acids from Red Alga Dulse in Japan
  Yuki Nishida; Yuya Kumagai; Shunta Michiba; Hajime Yasui; Hideki Kishimura
  Online International Symposium of FSMILE 2020 Current Trends on Food Processing, Safety and Nutrition, 24 Nov. 2020, English, Oral presentation
  24 Nov. 2020 - 25 Nov. 2020
• Healthy functional materials in red algae
  Yuya Kumagai; Manami Kobayashi; Hajime Yasui; Hideki Kishimura
  Online International Symposium of FSMILE 2020 Current Trends on Food Processing, Safety and Nutrition, 24 Nov. 2020, English, Invited oral presentation
  24 Nov. 2020 - 25 Nov. 2020, [Invited]
• 「海藻の秘密兵器！？「マイコスポリン様アミノ酸」
  西田有輝・熊谷祐也・岸村栄毅
  アイデアと街を繋ぎ函館の未来を創る 合同研究発表会, 08 Feb. 2020, Japanese, Nominated symposium
  [Invited]
• 紅藻に含まれる健康機能性成分
  熊谷祐也
  次世代食育のグローバル化フォーラム（FFSMILE2020）, 07 Feb. 2020, Japanese, Nominated symposium
  [Invited]
• A search for β-(1→3)/β-(1→4)-xylotriose degradation enzyme from Bifidobacterium adolescentis
  Manami Kobayashi; Yuya Kumagai; Hideki Kishimura
  12th Joint International Symposium on Food Science and Technology among NUS, TUMSAT, HU, KU and ZGU, 01 Dec. 2019, English, Oral presentation
• ボーっと海苔食べてると答えられない⁉「フィコビリタンパク質って？」
  森河理絵; 澄川果奈; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2019, 09 Nov. 2019
• ナマコのヌメヌメ「フコイダン」
  瀬能拓海; 栗田大輝; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2019, 09 Nov. 2019
• 最強のマースおにいさんはただひとり！オレだ！
  西田有輝; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2019, 09 Nov. 2019
• 紅藻ウップルイノリのACE阻害ペプチドの探索
  渡邊慧; 勝倉 智; 岡田千晃; 熊谷祐也; 岸村栄毅
  第5回 北海道大学部局横断シンポジウム, 06 Nov. 2019
• Bifidobacterium adolescentisのβ-(1→3)/β-(1→4)-キシロトリオース分解機構に関する研究
  小林真奈美; 熊谷祐也; 岸村栄毅
  第5回 北海道大学部局横断シンポジウム, 06 Nov. 2019
• 紅藻ダルスの糖質成分の活用について
  熊谷祐也
  海藻活用研究会 第3回定期シンポジウム, 12 Jul. 2019
  [Invited]
• 紅藻ダルス由来β-(1→3)/β-(1→4)-キシロトリオースの Bifidobacterium属細菌選択増殖作用の検討
  山本陽平; 小林真奈美; 熊谷祐也; 山崎浩司; 安井 肇; 尾島孝男; 岸村栄毅
  平成31年度日本水産学会春季大会, 27 Mar. 2019
• 函館産紅藻ダルス由来マイコスポリン様アミノ酸の 熱安定性および機能性
  道場俊太; 木下康宜; 安井 肇; 熊谷祐也; 岸村栄毅
  平成31年度日本水産学会春季大会, 27 Mar. 2019
• 紅藻ダルスのタンパク質に含まれる機能性ペプチドの探索
  熊谷祐也; 岸村栄毅
  第4回 北大・部局横断シンポジウム, 25 Jan. 2019
• 函館産ダルス由来マイコスポリン様アミノ酸の 含有量・組成の時期変動及び安定性に関する検討
  道場俊太; 武田朋之; 木下康宣; 安井 肇; 栗原秀幸; 熊谷祐也; 岸村栄毅
  日本食品科学工学会 北海道支部大会, 02 Dec. 2018
• 実はこんなにすごい！！ スーパーフード "ダルス"
  栗田大輝; 熊谷祐也; 岸村栄毅
  合同研究発表会 シエスタハコダテ編 ademikku〜HAKODATEアカデミックリンク2018連携〜 アイデアと街を繋ぎ箱館の未来を創る, 23 Nov. 2018
  [Invited]
• 実はこんなにすごい！！ スーパーフード "ダルス"
  栗田大輝; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2018, 10 Nov. 2018
• 紅藻"ダルス"は"第２のガゴメ"となりうるか
  藤井勇樹; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2018, 10 Nov. 2018
• 絶対に捨てられない海藻がここにある
  小林真奈美; 山本陽平; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2018, 10 Nov. 2018
• 紅藻の真価 〜北海道より〜
  岸本堯大; 熊谷祐也; 岸村栄毅
  HAKODATEアカデミックリンク2018, 10 Nov. 2018

• 海洋生物資源科学特別実習Ⅱ, 2024年, 修士課程, 水産科学院
• Introduction to Fisheries Sciences Ⅱ（水産科学汎論Ⅱ）, 2024年, 修士課程, 水産科学院
• 海洋応用生命科学特別実習Ⅱ, 2024年, 修士課程, 水産科学院
• 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
• 水産科学特別実習Ⅱ, 2024年, 修士課程, 水産科学院
• 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
• 食品工学, 2024年, 学士課程, 水産学部
• 生物化学実験, 2024年, 学士課程, 水産学部
• 健康と社会, 2024年, 学士課程, 全学教育
• 分析化学, 2024年, 学士課程, 水産学部
• 環境と人間, 2024年, 学士課程, 全学教育
• 生産プロセス工学実習, 2024年, 学士課程, 水産学部
• 分析化学, 2024年, 学士課程, 水産学部

• Apr. 2021 - Present
  日本水産学会
• 日本生物工学会
• 日本農芸化学会

• 低利用資源・紅藻類からの抗酸化物質の調製
  岸村栄毅; 熊谷祐也, 20 Jan. 2023 - Present, [Educational materials]
• ガゴメのサンプル処理
  岸村栄毅; 熊谷祐也, 26 May 2022 - Present, [Educational materials]
• 紅藻ウップルイノリ・タンパク質に含まれる新規機能性ペプチドの同定
  岸村栄毅; 熊谷祐也, 25 May 2022 - Present, [Educational materials]
• 低利用資源・紅藻から紫外線防御物質の調製法の開発
  岸村栄毅; 熊谷祐也, 25 May 2022 - Present, [Educational materials]
• 未利用資源紅藻ダルスとその有効活用に向けて
  岸村栄毅; 熊谷祐也, 25 May 2022 - Present, [Educational materials]
• ダルスの採集
  岸村栄毅; 熊谷祐也, 29 Mar. 2022 - Present, [Educational materials]
• ワカメの採集
  岸村栄毅; 熊谷祐也, 29 Mar. 2022 - Present, [Educational materials]
• 生産プロセス工学実習（連続蒸留理論・冷凍工学理論）【実験動画】
  LASBOS Moodle, 岸村栄毅; 熊谷祐也, 21 Oct. 2021 - Present, [Educational materials]
• 低利用資源・紅藻から新たなオリゴ糖調製法の開発2
  LASBOS Moodle, 岸村栄毅; 熊谷祐也, 21 Oct. 2021 - Present, [Educational materials]
• アカデミックリンク2020・紅藻ダルスへの質問・ご意見
  LASBOS Moodle, 熊谷祐也, 21 Oct. 2021 - Present, [Educational materials]
• Search for health functional ingredients in red algae, a low-use resource; protein and peptide
  LASBOS Moodle, Hideki Kishimura; Yuya Kumagai, 21 Oct. 2021 - Present, [Educational materials]
• Search for health functional ingredients in red algae, a low-use resource; a method for preparing new oligosaccharides
  LASBOS Moodle, Hideki Kishimura; Yuya Kumagai, 21 Oct. 2021 - Present, [Educational materials]
• Genome analysis of mitochondria and chloroplast from red alga dulse and the relation of bioactivity
  LASBOS Moodle, Hideki Kishimura; Yuya Kumagai, 21 Oct. 2021 - Present, [Educational materials]

• コムギのゲノム編集方法、及びその利用
  Patent right, 柳楽洋三; 濱田晴康; 三木隆二; 田岡直明; 今井亮三; 熊谷祐也
  特願2020-151998, 10 Sep. 2020
  特許WO/2021/070549

• 令和4年度 公益社団法人日本農芸化学会 北海道・東北支部合同若手の会
  20 Sep. 2022 - 21 Sep. 2022
  Organizing member
  公益社団法人日本農芸化学会 北海道支部
• Reviewer Certificate
  Jun. 2022
  Organizing member
  日本水産学会誌・FISHERIES SCIENCE
• Reviewer Certificate
  10 Sep. 2020
  Organizing member
  MDPI・Molecules
• Reviewer Certificate
  28 Jun. 2020
  Organizing member
  ELSEVIER・Carbohydrate Research
• Reviewer Certificate
  29 Sep. 2019
  Organizing member
  MDPI・Marine Drugs
• Reviewer Certificate
  11 Sep. 2019
  Organizing member
  Springer・Fishries Science
• 海藻の光る成分を調べてみよう！
  05 Aug. 2019
  Lecturer
  北海道大学大学院水産科学研究院 2019
  北海道大学水産学部オープンキャンパス 2019年
• Reviewer Certificate
  19 Jul. 2019
  Organizing member
  ELSEVIER・Biochimie
• Reviewer Certificate
  18 Jul. 2019
  Organizing member
  MDPI・Marine Drugs
• Reviewer Certificate
  10 Jun. 2019
  Organizing member
  J-Stage・Bioscience, Biotechnology, and Biochemistry

• 海藻「ダルス」活用模索 コンブ養殖の厄介者 血糖値抑える糖質生成
  Aug. 2024
  北海道新聞
  北海道新聞
  [Paper]
• 水産学部とシンガポール国立大学のサマーコースの取組みが北海道新聞に掲載されました
  Jun. 2024
  北海道大学ホームページ
  北海道大学ホームページ
  プレスリリース, [Internet]
• 色落ちした紅藻"ダルス"から血糖抑制が期待されるオリゴ糖を調製～低利用資源の有効活用への貢献に期待～
  May 2024
  北海道大学ホームページ
  北海道大学ホームページ
  プレスリリース, [Internet]
• 低利用資源の紅藻"ダルス"から紫外線防御物質MAAs調製法を確立 ～効率的な調製法を検討、異なる条件で含有量と組成を比較～
  Oct. 2023
  北海道大学ホームページ
  北海道大学ホームページ
  プレスリリース, [Internet]
• 北海道大学－シンガポール国立大学 サマーコース ～日本とシンガポールにおける水産物供給体制の比較～
  Jun. 2020
  北海道大学 教育改革室
  北海道大学における教育方法のグッド・プラクティス
  [Pr]