Researcher basic information

• D.V.M.Ph.D., Hokkaido University, Mar. 2009

• https://researchmap.jp/read0140611?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901045319589831
• https://orcid.org/0000-0002-6643-1554
• http://www.vetmed.hokudai.ac.jp/organization/anat/index.html

• 60547769

• https://orcid.org/0000-0002-6643-1554

• 200901045319589831

• Animal
• Kidney
• 泌尿器
• Glomerulus
• Tubulointerstitium
• Urine
• Immunity

• Life Science, Veterinary medical science
• Life Science, Animal life science
• Life Science, Anatomy
• Life Science, Morphology and anatomical structure
• Life Science, Nephrology
• Life Science, Urology
• Life Science, Connective tissue disease and allergy

• Bachelor's degree program, School of Veterinary Medicine
• Doctoral (PhD) degree program, Graduate School of Veterinary Medicine

Research activity information

• Sep. 2023, 日本獣医学会, Good Reviewer賞
• Oct. 2022, アジア獣医解剖学会, 第8回 アジア獣医解剖学会 Best Oral Presentation Award
  Activity of Technical Workshop for Animal Gross Anatomy in the Japanese Association of Veterinary Anatomists
• Sep. 2018, The Japanese Society of Veterinary Science, The Japanese Society of Veterinary Science Award
  Studies on the novel marker molecules for kidney diseases and their application to clinical veterinary medicine
• Mar. 2017, Kazato Research Foundation, Kazato Research Encouragement Prize
  走査型電子顕微鏡の応用的手法による腎臓病理解析法の革新
• Mar. 2013, Japanese Association of Veterinary Anatomists, Best poster award in Japanese Association of Veterinary Anatomists
  腎臓病のmicroRNA解析―動物種共通のバイオマーカー開発を目指して―
• Apr. 2011, Leave a Nest Co.,ltd., Leave a Nest Toray award
  種の壁を越えた尿中microRNAバイオマーカーの開発
• Mar. 2009, Japanese Society of Veterinary Science, Veterinary Science Young Investigator Award
  腎疾患の新しい診断指標－Interleukin 1 family,member 6 は尿細管傷害マーカーになり得る－
  Japan
• Mar. 2009, Japanese Society of Veterinary Science, Award from the chairman
  腎疾患の新しい診断指標－Interleukin 1 family,member 6 は尿細管傷害マーカーになり得る－
  Japan
• Mar. 2008, Japanese Association of Veterinary Anatomists, Best presentation award in Japanese Association of Veterinary Anatomists
  性腺摘出が自己免疫性糸球体腎炎の病態に与える影響について
  Japan
• Sep. 2007, Japanese Association of Veterinary Anatomists, Award in Japanese Association of Veterinary Anatomists
  自己免疫性糸球体腎炎とFc gamma receptor III (Fcgr3)の関連性について
  Japan

• Three-dimensional structure and epithelial changes of the piriform sinus fistula in cotton rats
  Teppei Nakamura; Marina Hosotani; Osamu Ichii; Takao Irie; Akio Shinohara; Hirokazu Kouguchi; Yasuhiro Kon
  Cell and Tissue Research, 403, 3, Springer Science and Business Media LLC, 26 Feb. 2026
  Scientific journal
• Protoscolex Maturation in Echinococcus multilocularis Is Controlled by Host Genetic Factors Independent of Initial Development
  Moe Kogawa; Keisuke Sato; Teppei Nakamura; Naoki Hayashi; Hirokazu Kouguchi; Ryo Nakao; Masahito Hidaka; Hiroyuki Matsuyama; Nariaki Nonaka; Osamu Ichii; Takashi Agui; Masami Morimatsu
  Parasite Immunology, 48, 2, Wiley, 16 Feb. 2026
  Scientific journal, ABSTRACT
  
  Alveolar echinococcosis, caused by Echinococcus multilocularis , poses a serious public health concern. This parasite requires rodents as intermediate hosts for protoscolex maturation in the liver and subsequent transmission to definitive hosts. We aimed to identify the host genetic factors controlling protoscolex development and maturation in congenic mice carrying susceptible DBA/2 genomic segments on a resistant C57BL/6N background. We assessed the hepatic protoscoleces and immune‐related gene expression levels 16 weeks post‐oral infection. All congenic strains developed protoscoleces; however, the number and proportion of mature forms decreased as C57BL/6N‐derived genomic contribution on chromosome 1 increased. Differences in DBA/2‐derived segments revealed two loci, E. multilocularis protoscolex maturation 1 ( Empmat1 ) and 2 ( Empmat2 ), as regulators of protoscolex maturation, but not early development, where immune involvement was limited. T‐box transcription factor 21 expression was correlated with protoscolex maturation; however, it lay outside Empmat1 and Empmat2 and was unlikely to be the responsible gene. Therefore, protoscolex maturation is potentially regulated by multiple host genetic factors distinct from those involved in early development, with B6‐derived alleles exerting inhibitory effects, possibly via non‐immune host mechanisms. Elucidation of these genetic pathways can reveal novel targets to disrupt the life cycle and reduce the zoonotic transmission of E. multilocularis .
• Multi-laboratory Validation of Join of the Five Dyes Revealing Collagenous Tissue (JFRL) Staining for Bone Histology Across Animal Species and Bone Implant Materials.
  Erika Tsuji; Kanako Sumi; Natsuko Tsuzuki; Daisuke Kondoh; Masashi Tsujio; Marina Hosotani; Takashi Namba; Shoichi Wakitani; Osamu Ichii; Ko Nakanishi; Teppei Nakamura
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 31, 4, 15 Jul. 2025, [International Magazine]
  English, Scientific journal, Histological staining is essential for understanding bone structure and pathology; however, variations in decalcification agents can compromise reproducibility. We have previously developed a novel osteochondral staining method, Join of the Five dyes Revealing coLlagenous tissue (JFRL) staining, that is independent of the decalcification method. To promote its widespread adoption, this study confirms the robustness of JFRL staining through intra- and inter-laboratory validation. JFRL staining demonstrated consistent patterns across different manufacturers and facilities, with proper dehydration steps being crucial for optimal results. We applied JFRL staining to diverse vertebrate species prepared under various fixation and decalcification conditions to effectively visualize species-specific bone structures, including distinct osteoid and mineralized bone features from fish to large mammals. Furthermore, JFRL staining proved useful in evaluating bone biomaterials within defect models and clearly depicts the complex architecture of bone-healing processes and material integration. The staining qualitatively distinguished osteoid, mineralized bone, hyaline cartilage, and bone cells of different colors across all applications. These findings establish JFRL staining as a robust and versatile method for bone histology. Future studies focusing on quantitative assessment and pathological applications will prove that JFRL staining presents a reliable tool for both basic research and clinical diagnostics of bone disorders.
• Altered morphology of mucosa-associated lymphoid tissues and epithelium in the nasal cavity and lacrimal apparatus in autoimmune disease-prone MRL/MpJ-Faslpr/lpr mice
  Masaya Hiraishi; Takashi Namba; Teppei Nakamura; Md. Zahir Uddin Rubel; Yasuhiro Kon; Osamu Ichii
  Cell and Tissue Research, Springer Science and Business Media LLC, 25 Mar. 2025
  Scientific journal
• Application of chlorous acid water for disinfection of surgical site in dairy cows.
  Osamu Ichii; Teppei Nakamura; Masaya Hiraishi; Takashi Namba; Md Zahir Uddin Rubel; Takuya Umeyama; Megumi Asai
  Frontiers in veterinary science, 12, 1444674, 1444674, 2025, [International Magazine]
  English, Scientific journal, Disinfection is crucial for preventing surgical site infections. Recently, the effectiveness of sanitizers using chlorous acid (HClO2) under conditions rich in organic matter has been reported, and chlorous acid water (CAW) has been approved as a food additive. This study evaluated the potential of CAW as a new presurgical disinfectant for cattle. The experiments were performed on the paralumbar fossa of cattle in Sapporo during March (winter to spring) and August (summer). Colony-forming units (CFUs) of standard plate count bacteria (SPCB), Enterococcus faecalis (EF), Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus spp. (SP) were analyzed as indicators of bacterial load. SPCB and SP were abundantly detected, exceeding 6 log10 CFU/100 g on clipped hair and 6 log10 CFU/100 cm2 on the skin immediately after clipping, with no significant seasonal differences. The bacterial load on the skin was evaluated at three time points: after clipping, cleansing, and disinfection. Clipping and cleansing with liquid soap were common procedures, following this, either the standard disinfection protocol using 7.5% iodine scrub for 1 min, 10% povidone-iodine for 5 min, and 70% alcohol for 5 min (SPA), or a modified protocol using CAW with contact times of 15, 10, or 5 min (CAW15, CAW10, CAW5) were performed separately. The cleansing procedure significantly reduced the SPCB, EF, and SP on the skin after clipping, and all disinfection methods significantly decreased the SP after cleansing. Draping significantly enhanced the disinfection efficiency of the SPA, CAW10, and CAW5 protocols. The CAW procedure did not alter skin histology in the paralumbar fossa or udder compared to 10% povidone-iodine or 70% alcohol. Our data suggest that the disinfection method using CAW is useful and comparable to routine disinfection methods and might reduce the time required for presurgical disinfection in farm fields.
• Effects of autoimmune abnormalities on fertility and placental morphology in mice.
  Risa Yamanaka; Osamu Ichii; Teppei Nakamura; Yuki Otani; Takashi Namaba; Yasuhiro Kon
  Autoimmunity, 57, 1, 2319209, 2319209, Dec. 2024, [International Magazine]
  English, Scientific journal, Autoimmune diseases (AIDs) alter the placental immune environment leading to fetal loss. This study investigated the effects of AIDs on pregnancy and the placenta in AID-prone MRL/MpJ-Faslpr/lpr mice and wild-type MRL/MpJ, which were mated with male MRL/MpJ and MRL/MpJ-Faslpr/lpr at five months and defined as moLpr and moMpJ, respectively. AID indices (spleen weight and serum autoantibody levels) and fertility status (number and size of fetuses, morphology, and comprehensive gene expression of placentas) were evaluated on gestational day 15.5. Both strains showed equivalent fertility, but moLpr showed lighter placentas and fetuses than moMpJ, and decreased fertility with AID severity. moLpr placentas had a higher number of T cells, higher expression of genes associated with T helper 2 and T follicular helper functions, and altered expression of genes (Krt15, Slc7a3, Sprr2a3) that significantly regulate pregnancy or immunity. The gene expression of T cell migration-associated chemokines (Ccl5, Cxcl9) was significantly increased in moLpr placentas, and CCL5 and CXCL9 were detected in moLpr placentas, particularly in T cells and placenta-component cells, respectively. Thus, AID altered placental morphofunction and fertility in mice; however, fertility was maintained at the examined time points. This study enhances our understanding of placental alterations and gestational risk due to AIDs.
• Salinity negatively correlates with the production and immunity of chicken: A molecular insight for food security and safety issues.
  Subrato Biswas; Md Abdul Masum; Sujan Kumar Sarkar; Basant Saud; Rupa Akter; K B M Saiful Islam; Shah Jungy Ibna Karim; Md Mostafizur Rahman; Hossain M Golbar; Md Emtiaj Alam; Md Akhtarul Islam; Maksuda Begum; Mohammad Musfiqur Rahman; Osamu Ichii; Yasuhiro Kon
  Heliyon, 10, 15, e34819, 15 Aug. 2024, [International Magazine]
  English, Scientific journal, Salinity intrusion into the freshwater system due to climate change and anthropogenic activities is a growing global concern, which has made humans and domesticated animals more susceptible to diseases, resulting in less productivity. However, the effects of salinity on domesticated and wild birds, especially in terms of production and immunity, have not been fully elucidated yet. Therefore, this study was designed to examine the effects of salinity on the production and immunity of birds and the mechanisms by which immunity is compromised. Broiler chicks were subjected to different concentrations of salty water (control = normal water, treatment = 5 g/L, treatment = 10 g/L, and treatment = 15 g/L). The collected blood and organs from different groups of broilers were biochemically and histopathologically examined. Birds in salt-treated groups consumed significantly less feed than the control group, while the feed conversion ratio (FCR) was significantly higher. Body weight gain was significantly lower in salt-treated groups compared to control. Serum analysis revealed a lower systemic antibody titer in the salt-treated groups compared to the control. Primary lymphoid organs (thymus and bursa of Fabricius) were reduced in size in the salt-treated group due to cellular migration and depletion from these organs. Importantly, most of the parenchyma of lymphoid organs was replaced with fibrotic tissue. Gut microbes, Escherichia coli (E. coli) and Salmonella spp., from salt-treated groups, showed less viability but developed antibiotic resistance. Levels of salinity were significantly and negatively correlated with feed intake, body weight gain, antibody titer, lymphoid organ size, and viable count of gut microbes, while FCR, fibrosis of lymphoid organs, and antibiotic resistance were significant positively correlated. In conclusion, increased salinity is a possible threat to food security and safety as it decreases body weight gain, reduces immunity, and influences the development of multi-drug resistance in gut microbes.
• Lipidomic study of kidney in a mouse model with urine flow obstruction.
  Divyavani Gowda; Md Abdul Masum; Siddabasave Gowda B Gowda; Chandra Shekhar; Md Zahir Uddin Rubel; Shunnosuke Kira; Osamu Ichii; Yasuhiro Kon; Hitoshi Chiba; Shu-Ping Hui
  Scientific reports, 14, 1, 18042, 18042, 05 Aug. 2024, [International Magazine]
  English, Scientific journal, Obstructed urine flow is known to cause structural and functional kidney damage leading to renal fibrosis. However, limited information is available on the change in kidney lipids during urinary tract obstruction. In this study, we investigated the change in lipidome in a mouse model with unilateral ureteral obstruction (UUO). The establishment of the UUO model was confirmed by histopathological examination using transmission electron microscopy. Untargeted liquid chromatography/mass spectrometry was carried out over a time course of 4 and 7 days. Compared to the sham control, the UUO kidney at 7 days showed dilatation of the renal tubule with loss of brush borders and thickening of the capillary endothelium. In the kidney lipidomes obtained from the UUO 7 days group compared to the control, a significant decrease of ceramide, sphingomyelin, phosphatidylcholine, lysophospholipids, and phosphatidylethanolamine was observed, whereas cholesteryl esters, free fatty acids, phosphatidylglycerol, and cardiolipins were significantly increased. The present study revealed the disturbed lipid metabolism in the UUO model, which may provide a clue to potential lipid pathways and therapeutic targets for the early stage of renal fibrosis.
• Species-specific histological characterizations of renal tubules and collecting ducts in the kidneys of cats and dogs
  Shunnosuke Kira; Takashi Namba; Masaya Hiraishi; Teppei Nakamura; Yuki Otani; Yasuhiro Kon; Osamu Ichii
  PLOS ONE, 19, 7, e0306479, e0306479, Public Library of Science (PLoS), 03 Jul. 2024
  Scientific journal, The histomorphological features of normal kidneys in cats and dogs have been revealed despite the high susceptibility of cats to tubulointerstitial damage. Herein, the histological characteristics of the two species were compared. Cytoplasmic lipid droplets (LDs) were abundant in the proximal convoluted tubules (PCTs) of cats aged 23–27 months but scarce in dogs aged 24–27 months. LDs were rarely observed in the distal tubules (DTs) and collecting ducts (CDs) of either species, as visualized by the expression of Tamm–Horsfall protein 1, calbindin-D28K, and aquaporin 2. The occupational area ratio of proximal tubules (PTs) in the renal cortex was higher, but that of DTs or CDs was significantly lower in adult cats than in dogs. Single PT epithelial cells were larger, but PCT, DT, and CD lumens were significantly narrower in adult cats than in dogs. Unlike adults, young cats at 6 months exhibited significantly abundant cytoplasmic LDs in proximal straight tubules, indicating lipid metabolism-related development. Histochemistry of the 21 lectins also revealed variations in glycosylation across different renal tubules and CDs in both species. Sodium-glucose cotransporter 2 was expressed only in PTs, excluding the proximal straight tubules with few LDs in adult cats or the PCTs of young cats and adult dogs. These findings are crucial for understanding species-specific characteristics of renal histomorphology and pathogenesis.
• Collagen 17A1 in the Urothelium Regulates Epithelial Cell Integrity and Local Immunologic Responses in Obstructive Uropathy.
  Takashi Namba; Osamu Ichii; Ken Natsuga; Teppei Nakamura; Yuki Otani; Yasuhiro Kon
  The American journal of pathology, 18 May 2024, [International Magazine]
  English, Scientific journal, Collagen 17A1 (COL17A1), an epidermal hemidesmosome component, is ectopically induced in the urothelium of mouse and human renal pelvis (RP) in parallel with urinary tract-associated lymphoid structure development. Here, we found that COL17A1 was induced in obstructive uropathy-prone ureter of humans and cats. To ascertain its function, murine urinary organs with unilateral ureteral obstruction (UUO) were analyzed during 1 week after surgery. One day after UUO, COL17A1 expression increased in urothelial cells of RP and ureter, and was positively correlated with renal tubulointerstitial lesions. A portion of RP where the smooth muscle layer from the ureter was interrupted was sensitive to urothelium deciduation and COL17A1 induction, showing urine leaked from the RP lumen into the parenchyma. After urine stimulation, cultured immune cells expressed Cxcl2, also up-regulated in CD11b+ cells following COL17A1 stimulation. One day after UUO, CXCL2+ CD11b+ cells infiltrated the urothelium-disrupted area; however, these numbers were significantly lower in Col17a1-deficient mice. COL17A1+ urothelial cells partially co-expressed cytokeratin-14, a progenitor cell marker for urothelium, whereas Col17a1-deficient mice had lower numbers of cytokeratin-14+ cells. Gene Ontology analysis revealed that expression of epithelial- and immune-associated genes was up-regulated and down-regulated, respectively, in the ureter of Col17a1-deficient mice 4 days after UUO. Thus, COL17A1 maintains urothelium integrity by regulating urothelial cell adhesion, proliferation, and differentiation, and activates local immune responses during obstructive uropathy in mammals.
• Systemic autoimmune abnormalities alter the morphology of mucosa-associated lymphoid tissues in the rectum of MRL/MpJ-Faslpr/lpr mice.
  Md Zahir Uddin Rubel; Osamu Ichii; Takashi Namba; Md Abdul Masum; Tsolmon Chuluunbaatar; Masaya Hiraishi; Teppei Nakamura; Yasuhiro Kon
  Experimental animals, 02 Feb. 2024, [Domestic magazines]
  English, Scientific journal, Systemic autoimmune diseases (ADs) might affect the morphology and function of gut-associated lymphoid tissue (LTs) indirectly; however, their exact relationship remains unclear. Therefore, we investigated mouse LTs in the anorectal canal and morphologically compared them between MRL/MpJ-Fas+/+ and MRL/MpJ-Faslpr/lpr mice. LT aggregations, also known as rectal mucosa-associated lymphoid tissues (RMALTs), were exclusively seen in the lamina propria and submucosa of the rectum. The mean size and number of the LT aggregations both significantly increased in MRL/MpJ-Faslpr/lpr mice compared to those in MRL/MpJ-Fas+/+ mice. The distance from the anorectal junction to the first LT aggregate was significantly shorter in MRL/MpJ-Faslpr/lpr mice than that in MRL/MpJ-Fas+/+ mice. Immunostaining revealed that the RMALTs included CD3+,CD4+, and CD8+ T cells; B220+ B cells; IBA1+ macrophages; Ki67+ proliferative cells; and PNAd+ high-endothelial venules (HEVs). The numbers of macrophages, proliferative cells, CD4+ T cells, CD8+ T cells, and HEVs were significantly increased in MRL/MpJ-Faslpr/lpr mice compared to those in MRL/MpJ mice. Furthermore, the gene expression levels of chemokines (Cxcl9 and Cxcl13) and their corresponding receptors (Cxcr3 and Cxcr5) were significantly higher in MRL/MpJ-Faslpr/lpr mice than those in MRL/MpJ-Fas+/+ mice. Although the morphology of rectal epithelium was comparable between the strains, M cell number was significantly higher in MRL/MpJ-Faslpr/lpr mice than in MRL/MpJ-Fas+/+ mice. Thus, ADs could alter RMALT morphology, and quantitative changes in T-cell subsets, proliferative cells, macrophages, HEVs, chemokine expression, and M cells could affect their cell composition and development.
• Roles of tumor necrosis factor-like ligand 1A in γδT-cell activation and psoriasis pathogenesis
  Shangyi Wang; Mina Kozai; Masaya Hiraishi; Md. Zahir Uddin Rubel; Osamu Ichii; Mutsumi Inaba; Kazuhiro Matsuo; Kensuke Takada
  Frontiers in Immunology, 15, 1340467, 1340467, Frontiers Media SA, 29 Jan. 2024, [International Magazine]
  English, Scientific journal, Background
  
  Interleukin (IL)-17-producing γδT (γδT17) cells mediate inflammatory responses in barrier tissues. Dysregulated γδT17 cell activation can lead to the overproduction of IL-17 and IL-22 and the development of inflammatory diseases, including psoriasis. IL-23 and IL-1β are known to synergistically activate γδT17 cells, but the regulatory mechanisms of γδT17 cells have not been fully elucidated. This study aimed to reveal the contribution of the inflammatory cytokine tumor necrosis factor-like ligand 1A (TL1A) to γδT17 cell activation and psoriasis development.
  
  Methods
  
  Anti-TL1A antibody was injected into an imiquimod (IMQ)-induced murine psoriasis model. TL1A receptor expression was analyzed in splenic and dermal γδT cells. γδT cells were tested for cytokine production in vitro and in vivo under stimulation with IL-23, IL-1β, and TL1A. TL1A was applied to a psoriasis model induced by intradermal IL-23 injection. Mice deficient in γδT cells were intradermally injected with IL-23 plus TL1A to verify the contribution of TL1A-dependent γδT-cell activation to psoriasis development.
  
  Results
  
  Neutralization of TL1A attenuated γδT17 cell activation in IMQ-treated skin. TL1A induced cytokine production by splenic γδT17 cells in synergy with IL-23. Dermal γδT17 cells constitutively expressed a TL1A receptor at high levels and vigorously produced IL-22 upon intradermal IL-23 and TL1A injection but not IL-23 alone. TL1A exacerbated the dermal symptoms induced by IL-23 injection in wild-type but not in γδT cell–deficient mice.
  
  Conclusion
  
  These findings suggest a novel regulatory mechanism of γδT cells through TL1A and its involvement in psoriasis pathogenesis as a possible therapeutic target.
• Phenotypes of streptozotocin-induced gestational diabetes mellitus in mice.
  Narumi Takahashi; Osamu Ichii; Masaya Hiraishi; Takashi Namba; Yuki Otani; Teppei Nakamura; Yasuhiro Kon
  PloS one, 19, 4, e0302041, 2024, [International Magazine]
  English, Scientific journal, Gestational diabetes mellitus (GDM) in human patients disrupts glucose metabolism post-pregnancy, affecting fetal development. Although obesity and genetic factors increase GDM risk, a lack of suitable models impedes a comprehensive understanding of its pathology. To address this, we administered streptozotocin (STZ, 75 mg/kg) to C57BL/6N mice for two days before pregnancy, establishing a convenient GDM model. Pregnant mice exposed to STZ (STZ-pregnant) were compared with STZ-injected virgin mice (STZ-virgin), citrate buffer-injected virgin mice (CB-virgin), and pregnant mice injected with citrate buffer (CB-pregnant). STZ-pregnant non-obese mice exhibited elevated blood glucose levels on gestational day 15.5 and impaired glucose tolerance. They also showed fewer normal fetuses compared to CB-pregnant mice. Additionally, STZ-pregnant mice had the highest plasma C-peptide levels, with decreased pancreatic islets or increased alpha cells compared to CB-pregnant mice. Kidneys isolated from STZ-pregnant mice did not display histological alterations or changes in gene expression for the principal glucose transporters (GLUT2 and SGLT2) and renal injury-associated markers. Notably, STZ-pregnant mice displayed decreased gene expression of insulin-receiving molecules (ISNR and IGFR1), indicating heightened insulin resistance. Liver histology in STZ-pregnant mice remained unchanged except for a pregnancy-related increase in lipid droplets within hepatocytes. Furthermore, the duodenum of STZ-pregnant mice exhibited increased gene expression of ligand-degradable IGFR2 and decreased expression of GLUT5 and GLUT12 (fructose and glucose transporters, respectively) compared to STZ-virgin mice. Thus, STZ-pregnant mice displayed GDM-like symptoms, including fetal abnormalities, while organs adapted to impaired glucose metabolism by altering glucose transport and insulin reception without histopathological changes. STZ-pregnant mice offer a novel model for studying mild onset non-obese GDM and species-specific differences in GDM features between humans and animals.
• Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy Cows.
  Yuka Katayama; Osamu Ichii; Teppei Nakamura; Keita Yanase; Masaya Hiraishi; Takashi Namba; Yuki Otani; Teppei Ikeda; Erika Tsuji; Natsuko Tsuzuki; Ken Kobayashi; Yasuhiro Kon; Takanori Nishimura
  Animals : an open access journal from MDPI, 13, 23, 29 Nov. 2023, [International Magazine]
  English, Scientific journal, Foreign body reactions (FBRs) are inadvertently observed in invading or artificially embedded materials, triggering inflammation and subsequent fibrotic processes to occur in situ. Here, we assessed the spatiotemporal formation of connective tissue around implanted materials to establish a technique using connective tissue formed by FBRs as xenografts. An acrylic resin implant, comprising a columnar inner rod and a tubular outer cylinder (OC) with several slits, was embedded in adult dairy cows. Tissues formed in the inner rod and OC groups were histologically analyzed at weeks 2, 4, 8, and 12. Edematous tissues with non-collagenous fibers formed for 2 weeks and showed increased cellularity after 4 weeks. The weight, thickness, amounts of total protein, collagen, DNA, and quantitative scores of α-smooth muscle actin-positive myofibroblasts or elastic fibers notably increased after 8 weeks, with condensed collagen fibers showing orientation. Inflammatory cells were primarily localized in tissues close to the OC, and their numbers increased, with the count of CD204+ cells peaking at 8 weeks and declining at 12 weeks. The count of Ki67+ proliferating cells slightly increased in tissues close to the OC; however, the number and lumen of CD31+ vessels increased. These results may help understand FBR-related tissue remodeling.
• Histological Characteristics of Conjunctiva-Associated Lymphoid Tissue in Young and Adult Holstein Cattle.
  Keigo Kosenda; Osamu Ichii; Yusuke Yamashita; Hiromichi Ohtsuka; Shigeo Fukuda; Yasuhiro Kon
  Animals : an open access journal from MDPI, 13, 22, 11 Nov. 2023, [International Magazine]
  English, Scientific journal, The conjunctiva-associated lymphoid tissue (CALT) has been used as a target site for mucosal vaccinations in several animals. In this study, we compared the morphological features of CALT in the eyelid and third eyelid between Holstein calves and adult cows. In the eyelids, CALTs in the form of diffused lymphoid tissue (DLT) and lymphatic follicles (LF) were observed, where DLTs were dominant and LFs were scarce. The CALTs of cows comprised T-, B-cells, macrophages, and antigen-presenting cells (APCs). In particular, B-cells were dominant except in the eyelids of the calves. The epithelial layer covering the CALT is often discontinuous and lacks goblet cells. Cytokeratin18 is strongly expressed in the epithelial layer covering the CALT, except in the third eyelids of adult cows. IgA-positive cells were diffusely distributed in the lamina propria of the conjunctiva of the eyelids and third eyelids. The eyelid CALT area in calves was lower than that in adult cows. Furthermore, the CALT of calves had a lower cellularity of B-cells and a higher cellularity of macrophages than that of adult cows. These histological characteristics indicate that CALT plays a role in the mucosal immune-inductive and effector sites. Furthermore, lower cellularity of B-cells in the CALT of calves indicates that the function of CALT as a mucosal immune induction site is less developed in calves than in adult cows.
• Effects of autoimmune abnormalities on skeletal muscle regeneration after needle puncture in mice
  Misato Masugi; Osamu Ichii; Yuki Otani; Takashi Namba; Yasuhiro Kon
  Experimental Biology and Medicine, 248, 20, 1829, 1840, SAGE Publications, 26 Sep. 2023, [International Magazine]
  English, Scientific journal, Regeneration of injured skeletal muscles is supported by the activation of satellite cells, and excessive traumatic injuries may trigger abnormal processes, such as fibrosis. Because the participation of immune cells is crucial during skeletal muscle repair, systemic autoimmune diseases impair their regeneration. This study focused on a traumatic injury by injection and investigated the effect of autoimmune diseases on skeletal muscle regeneration. Male mice of MRL/MpJ- Fas^lpr/lpr and MRL/MpJ (6–7 months old) were used for autoimmune disease and healthy groups. The abdominal walls punctured by a needle were histologically analyzed at 1, 3, and 8 days postinjection. In both groups, injured skeletal muscle tissues showed necrosis and inflammatory cell infiltrations on day 1, increased cell density at 3 days, and regenerative myotubes with central nuclei without fibrosis at 8 days. Gr-1⁺ neutrophils at injured skeletal muscle were abundant at 1 day, and then substantially decreased starting from 3 days in both groups. The number of CD3⁺ T cells was remarkably higher in MRL/MpJ- Fas^lpr/lpr than that in MRL/MpJ at 1 day, and a similar tendency was observed in B220⁺ B cells. The numbers of IBA1⁺ macrophages and bromodeoxyuridine-incorporating cells tended to be higher at 3 days, and those of the latter, mainly proliferating paired-box-7⁺ satellite cells, showed significance at other time points and negatively correlated with the autoimmune disease indices, such as spleen weights or serum autoantibody level. Thus, this result suggested that injured skeletal muscle by minor trauma is normally regenerated regardless of the effects of autoimmune diseases, although lymphocyte infiltrations during these processes were more severe in MRL/MpJ- Fas^lpr/lpr.
• Metataxonomic Analysis of the Uterine Microbiota Associated with Low Fertility in Dairy Cows Using Endometrial Tissues Prior to First Artificial Insemination.
  Takuya Yagisawa; Jumpei Uchiyama; Iyo Takemura-Uchiyama; Shun Ando; Osamu Ichii; Hironobu Murakami; Osamu Matsushita; Seiji Katagiri
  Microbiology spectrum, 11, 3, e0476422, 15 Jun. 2023, [International Magazine]
  English, Scientific journal, The deterioration in reproductive performance in association with low fertility leads to significant economic losses on dairy farms. The uterine microbiota has begun to attract attention as a possible cause of unexplained low fertility. We analyzed the uterine microbiota associated with fertility by 16S rRNA gene amplicon sequencing in dairy cows. First, the alpha (Chao1 and Shannon) and beta (unweighted and weighted UniFrac) diversities of 69 cows at four dairy farms that had passed the voluntary waiting period before the first artificial insemination (AI) were analyzed with respect to factors including farm, housing style, feeding management, parity, and AI frequency to conception. Significant differences were observed in the farm, housing style, and feeding management, except parity and AI frequency to conception. The other diversity metrics did not show significant differences in the tested factors. Similar results were obtained for the predicted functional profile. Next, the microbial diversity analysis of 31 cows at a single farm using weighted UniFrac distance matrices revealed a correlation with AI frequency to conception but not with parity. In correlation with AI frequency to conception, the predicted function profile appeared to be slightly modified and a single bacterial taxon, Arcobacter, was detected. The bacterial associations related to fertility were estimated. Considering these, the uterine microbiota in dairy cows can be varied depending on the farm management practices and may become one of the measures for low fertility. IMPORTANCE We examined the uterine microbiota associated with low fertility in dairy cows derived from four commercial farms via a metataxonomic approach using endometrial tissues prior to the first artificial insemination. The present study provided two new insights into the relevance of uterine microbiota with respect to fertility. First, the uterine microbiota varied depending on housing style and feeding management. Next, a subtle change was observed in functional profile analysis: a formation of uterine microbiota was detected to be different in correlation with fertility in one farm studied. Considering these insights, an examination system on bovine uterine microbiota is hopefully established based on continuous research on this topic.
• Ameliorated Renal Pathological Feature in MRL/MpJ-Faslpr/lpr Background Interleukin-36 Receptor-Deficient Mice.
  Takashi Namba; Osamu Ichii; Tadashi Okamura; Kenta Nakano; Teppei Nakamura; Yuki Otani; Yasuhiro Kon
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 29, 2, 675, 685, 05 Apr. 2023, [International Magazine]
  English, Scientific journal, Systemic autoimmune diseases frequently induce lupus nephritis, causing altered balance and expression of interleukin 36 receptor (IL-36R) ligands, including agonists (IL-36α, β, γ) and antagonists (IL-36Ra, IL-38), in kidneys. Here, we established and analyzed a mouse model of lupus nephritis, MRL/MpJ-Faslpr/lpr with IL-36R-knockout (KO), compared to wild-type (WT) mice. In both genotypes, indices for immune abnormalities and renal functions were comparable, although female WT mice showed higher serum autoantibody levels than males. IL-36R ligand expression did not differ significantly between genotypes at the mRNA level or in IL-36α and IL-38 scores. However, glomerular lesions, especially mesangial matrix expansion, were significantly ameliorated in both sexes of IL-36R-KO mice compared to WT mice. Cell infiltration into the tubulointerstitium with the development of tertiary lymphoid structures was comparable between genotypes. However, the positive correlation with the IL-36α score in WT mice was not evident in IL-36R-KO mice. Fibrosis was less in female IL-36R-KO mice than in WT mice. Importantly, some IL-36α+ nuclei co-localized with acetylated lysine and GCN5 histone acetyltransferase, in both genotypes. Therefore, IL-36R ligands, especially IL-36α, contribute to the progression of renal pathology in lupus nephritis via IL-36R-dependent and IL-36R-independent pathways.
• Oocyte cumulus complex quality and oviduct transportation velocity in systemic autoimmune disease model mice.
  Marina Hosotani; Osamu Ichii; Takafumi Watanabe; Yasuhiro Kon
  Experimental biology and medicine (Maywood, N.J.), 248, 16, 15353702231160875, 15353702231160875, 24 Mar. 2023, [International Magazine]
  English, Scientific journal, Oocyte transportation by the oviduct involves the interaction between ciliated epithelial cells and cumulus cells. To determine whether the quality of cumulus-oocyte complexes (COCs) changes the transportation property of COCs, we compared the transportation velocity of COCs (TVC) by the infundibulum ex vivo with various combinations of infundibula and COCs collected from different mice. We used young and aged C57BL/6N and MRL/MpJ, and MRL/MpJ-Faslpr/lpr mice as the strains with intact female reproductive function and the systemic autoimmune disease model exhibiting oocyte pick-up dysfunction owing to the morphofunctional abnormality of ciliated epithelium, respectively. The TVC of aged MRL strains was less than that of aged C57BL/6N mice, suggesting that aging affects the transportation of COCs in MRL strains. The TVC of aged MRL/MpJ-Faslpr/lpr mice was the least among all examined combinations, whereas the TVC accelerated when the infundibulum or COCs were collected from other strains. These results indicate that the transportation property of COCs is determined not only by the ciliary function in the infundibulum but also by the properties of COCs.
• Analysis of gene expression in poultry red mite, Dermanyssus gallinae, by RNAscope in situ hybridization.
  Hikari Seo; Shiro Murata; Osamu Ichii; Takashi Namba; Shwe Yee Win; Takumi Sato; Eiji Oishi; Akira Taneno; Naoya Maekawa; Tomohiro Okagawa; Satoru Konnai; Kazuhiko Ohashi
  The Journal of veterinary medical science, 85, 5, 532, 535, 14 Mar. 2023, [Domestic magazines]
  English, Scientific journal, The poultry red mite (PRM; Dermanyssus gallinae) is a hematophagous ectoparasite that mainly infests chickens, and its infestation causes significant economic losses to the poultry industry. In this study, we examined the use of RNAscope-based in situ hybridization (ISH) to characterize gene expression in PRM. We analyzed the mRNA expression of Dermanyssus gallinaecathepsin D-1 (Dg-CatD-1) and Dermanyssus gallinae cystatin (Dg-Cys). RNAscope ISH analysis revealed that mRNA expression of Dg-CatD-1 was observed in the digestive tract, and Dg-Cystatin mRNA was expressed in the ovaries in addition to the digestive tract. RNAscope ISH could be applicable for the analysis of gene expression in each tissue of PRM and is an effective method to investigate the characteristics of target genes.
• Morphological Characteristics of Genital Organ-Associated Lymphoid Tissue in the Vaginal Vestibule of Goats and Pigs.
  Tsolmon Chuluunbaatar; Osamu Ichii; Md Abdul Masum; Takashi Namba; Yasuhiro Kon
  Veterinary sciences, 10, 1, 11 Jan. 2023, [International Magazine]
  English, Scientific journal, Mucosa-associated lymphoid tissue (MALT) is a specialized form of peripheral lymphoid tissue (LT), which is found on mucosal surfaces exposed to the environment. However, morphological data of these tissues in farm animals are scarce. This study investigated the gross anatomical and histological features of genital organ-associated lymphoid tissues (GOALTs) in the vaginal vestibule (VV) of healthy, non-pregnant, adult goats and pigs. Their VVs were composed of stratified squamous, non-keratinized epithelium, and various-sized dark-blue hematoxylin-positive spots were observed in whole-mount specimens, which were diffusely distributed throughout the mucosal surfaces. These spots were histologically identified as LTs and consisted of lymphatic nodules (LNs) or diffuse lymphoid tissue (DLTs). Both LNs and DLTs contained B cells, T cells, macrophages, dendritic cells, plasma cells, and high endothelial venules. Only the numbers of B cells were significantly higher in both the LNs and DLTs of pigs compared to goats. Furthermore, the surface of the VV epithelium covering the LTs was partially disrupted with a large intercellular space containing abundant connective tissue fibers with numerous lymphocytes. In conclusion, GOALTs in the VV appear to be common local immunological barriers in both examined animals. This knowledge is crucial for understanding the structures and disorders of female reproductive organs in farm animals.
• Transcriptome and proteome analysis of dogs with precursor targeted immune-mediated anemia treated with splenectomy.
  Mei Sugawara-Suda; Keitaro Morishita; Osamu Ichii; Takashi Namba; Keisuke Aoshima; Yumiko Kagawa; Sangho Kim; Kenji Hosoya; Nozomu Yokoyama; Noboru Sasaki; Kensuke Nakamura; Jumpei Yamazaki; Mitsuyoshi Takiguchi
  PloS one, 18, 5, e0285415, 2023, [International Magazine]
  English, Scientific journal, Precursor-targeted immune-mediated anemia (PIMA) in dogs is characterized by persistent non-regenerative anemia and ineffective erythropoiesis, and it is suspected to be an immune-mediated disease. Most affected dogs respond to immunosuppressive therapies; however, some are resistant. In this study, we carried out splenectomy as an alternative therapy for refractory PIMA in dogs, and analyzed gene expression levels in the spleen of dogs with or without PIMA and in serum before and after splenectomy. A total of 1,385 genes were found to express differentially in the spleens from dogs with PIMA compared with healthy dogs by transcriptome analysis, of which 707 genes were up-regulated, including S100A12, S100A8, and S100A9 that are linked directly to the innate immune system and have been characterized as endogenous damage-associated molecular patterns. Furthermore, immunohistochemistry confirmed that S100A8/A9 protein expression levels were significantly higher in dogs with PIMA compared with those in healthy dogs. A total of 22 proteins were found to express differentially between the serum samples collected before and after splenectomy by proteome analysis, of which 12 proteins were up-regulated in the samples before. The lectin pathway of complement activation was identified by pathway analysis in pre-splenectomy samples. We speculated that S100A8/9 expression may be increased in the spleen of dogs with PIMA, resulting in activation of the lectin pathway before splenectomy. These findings further our understanding of the pathology and mechanisms of splenectomy for PIMA.
• Biological effects related to exposure to polychlorinated biphenyl (PCB) and decabromodiphenyl ether (BDE-209) on cats
  Khidkhan K; Mizukawa H; Ikenaka Y; Nakayama SMM; Nomiyama K; Yokoyama N; Ichii O; Takiguchi M; Tanabe S; Ishizuka M
  PLOS ONE, 18, 1, e0277689, Jan. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, As an animal familiar to humans, cats are considered to be sensitive to chemicals; cats may be exposed to polychlorinated biphenyls (PCBs) and decabromodiphenyl ether (BDE-209) from indoor dust, household products, and common pet food, leading to adverse endocrine effects, such as thyroid hormone dysfunction. To elucidate the general biological effects resulting from exposure of cats to PCBs and PBDEs, cats were treated with a single i.p. dose of a principal mixture of 12 PCBs and observed for a short-term period. Results revealed that the testis weight, serum albumin, and total protein of the treated group decrease statistically in comparison with those in the control group. The negative correlations suggested that the decrease in the total protein and albumin levels may be disturbed by 4'OH-CB18, 3'OH-CB28 and 3OH-CB101. Meanwhile, the serum albumin level and relative brain weight decreased significantly for cats subjected to 1-year continuous oral administration of BDE-209 in comparison to those of control cats. In addition, the subcutaneous fat as well as serum high-density lipoprotein (HDL) and triglycerides (TG) levels increased in cats treated with BDE-209 and down-regulation of stearoyl-CoA desaturase mRNA expression in the liver occurred. These results suggested that chronic BDE-209 treatment may restrain lipolysis in the liver, which is associated with lipogenesis in the subcutaneous fat. Evidence of liver and kidney cell damage was not observed as there was no significant difference in the liver enzymes, blood urea nitrogen and creatinine levels between the two groups of both experiments. To the best of our knowledge, this is the first study that provides information on the biochemical effects of organohalogen compounds in cats. Further investigations on risk assessment and other potential health effects of PCBs and PBDEs on the reproductive system, brain, and lipid metabolism in cats are required.
• Lectin histochemistry of the normal feline kidney
  Noguchi A; Kurahara N; Yamato O; Ichii O; Yabuki A
  Vet Sci, 10, 1, 26, MDPI, Jan. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Lectins have a strict binding specificity to carbohydrate moieties of cellular components, and can thus indicate changes in the glycosylation of cells in diseases. However, lectin-binding patterns in nephron segments of feline kidneys have not been fully surveyed. The present study reported lectin-binding patterns in normal feline kidneys by histochemical investigations of eight commercially available lectin detection kits. Kidneys from four normal cats (intact males, 23–27 months old) were fixed in 4% paraformaldehyde, and embedded in paraffin; lectin histochemistry was performed for WGA, s-WGA, RCA-I, ConA, PNA, SBA, DBA, and UEA-I lectins. WGA, RCA, and ConA binding was observed from Bowman’s capsule to the collecting ducts, while only WGA was detected in the glomerular capillary. s-WGA was observed from the proximal tubules to the collecting ducts, showing discriminative heterogeneous binding. PNA and SBA were detected in the distal nephrons, such as the thin limbs of the loops of Henle, distal tubules, and collecting ducts. UEA-I binding was observed in the thick ascending limbs of the loops of Henle, especially in the macula densa regions. DBA lectin showed no positive labeling in nephrons. The observed binding patterns may prove beneficial for the analysis of changes in glycosylation in feline kidney diseases.
  Keywords: cat; kidney; lectin histochemistry; nephron segment
• Expression of Indian hedgehog signaling in murine oviductal infundibulum and its relationship with epithelial homeostasis
  Marina Hosotani; Osamu Ichii; Takashi Namba; Md. Abdul Masum; Teppei Nakamura; Yasuhiro Hasegawa; Takafumi Watanabe; Yasuhiro Kon
  Cell and Tissue Research, 391, 3, 595, 609, Springer Science and Business Media LLC, 29 Dec. 2022, [International Magazine]
  English, Scientific journal, Homeostasis of the oviductal infundibulum epithelium is continuously regulated by signaling pathways under physiological and pathological conditions. Herein, we investigated the expression of hedgehog (Hh) signaling-related components in the murine oviductal infundibulum, which is known to maintain homeostasis in the adult epithelium. Additionally, using autoimmune disease-prone MRL/MpJ-Faslpr/lpr (MRL/lpr) mice showing abnormal morphofunction of the ciliated epithelium of the infundibulum related to the oviductal inflammation, we examined the relationship between Hh signaling and pathology of the infundibulum. The expression and localization of Pax8, a marker for progenitor cells in the oviductal epithelium, and Foxj1, a marker for ciliogenesis, were examined in the infundibulum. The results showed that Pax8 was downregulated and Foxj1 was upregulated with aging, suggesting that homeostasis of the infundibulum epithelium of MRL/lpr mice was disturbed at 6 months of age. In all mice, the motile cilia of ciliated epithelial cells in the infundibulum harbored Hh signaling pathway-related molecules: patched (Ptch), smoothened (Smo), and epithelial cells harbor Gli. In contrast, Ptch, Smo, and Gli2 were significantly downregulated in the infundibulum of MRL/lpr mice at 6 months of age. The expression levels of Pax8 and Foxj1 were significantly positively correlated with those of Ptch1, Smo, and Gli2. Hh signaling is thought to be involved in homeostasis of the ciliated epithelium in the infundibulum. In MRL/lpr mice, which show exacerbated severe systemic autoimmune abnormalities, molecular alterations in Hh signaling-related components are considered to interact with local inflammation in the infundibulum, leading to disturbances in epithelial homeostasis and reproductive function.
• Ureteral morphology and pathology during urolithiasis in cats.
  Osamu Ichii; Kazuhisa Oyamada; Hazuki Mizukawa; Nozomu Yokoyama; Takashi Namba; Yuki Otani; Yaser Hosny Ali Elewa; Noboru Sasaki; Teppei Nakamura; Yasuhiro Kon
  Research in veterinary science, 151, 10, 20, 10 Dec. 2022, [International Magazine]
  English, Scientific journal, Cats exhibit high susceptibility to urinary organ-related diseases. We investigated the healthy ureter morphologies and compared these with ureters that were surgically resected distal to a urolithiasis obstruction in cats. Healthy ureters (total length 9.88 ± 0.38 cm) developed adventitia composed of collagen fibers (ADCF), containing a longitudinal muscular layer, toward the distal segment. The healthy ureter was the smallest in the middle segment (4.71-6.90 cm from the urinary bladder) with significantly decreased luminal and submucosal areas compared to those in the proximal segment. Diseased cats exhibited a high incidence of calcium oxalate urolithiasis with renal dysfunction, regardless of age, sex, and body size. Diseased ureters showed increased perimeters, inflammation, and decreased nerves in ADCF. Collagen fibers were increased in the submucosal area, intermuscular spaces, and ADCF, particularly near the obstructed lesion. The mean resected ureter length was 5.66 ± 0.49 cm, suggesting a high obstruction risk in the middle segment. The middle segment also increased the cross-sectional area of the ureter and ADCF, regardless of the distance from the obstructed lesion. The ureters in several cases either lacked the transitional epithelium, or exhibited transitional epithelial hyperplasia, and some of these formed the mucosal folds. In conclusion, we demonstrated the following characteristics and histopathological features of cat ureters: decreases in the ureter size, lumen area, and submucosa area from proximal to middle segment in healthy; ADCF changes in urolithiasis, including increased connective tissues with inflammation and decreased nerves. These data are important to understand the pathogenesis of feline ureteral obstruction.
• Anti-lipoprotein lipase antibody-associated autoimmune hypertriglyceridaemia in a patient with systemic lupus erythematosus
  Horino, T.; Inotani, S.; Matsumoto, R.; Ohnishi, H.; Komori, M.; Ichii, O.; Terada, Y.
  Lupus, 31, 11, 1408, 1409, SAGE Publications, Oct. 2022, [Peer-reviewed]
  Scientific journal
• [Not Available].
  Taro Horino; Satoshi Inotani; Reiko Matsumoto; Hiroshi Ohnishi; Masahiro Komori; Osamu Ichii; Yoshio Terada
  Lupus, 31, 11, 1408, 1409, Oct. 2022, [International Magazine]
  English
• Letter to the Editor
  Taro Horino; Satoshi Inotani; Reiko Matsumoto; Hiroshi Ohnishi; Masahiro Komori; Osamu Ichii; Yoshio Terada
  Lupus, 31, 11, 1408, 1409, SAGE Publications, Oct. 2022
  Scientific journal
• Primary Superior Lumbar Hernia with Nephrotic-range Orthostatic Proteinuria.
  Taro Horino; Takeshi Kashio; Satoshi Inotani; Sachi Yamaguchi; Masayuki Ishihara; Osamu Ichii; Yoshio Terada
  Internal medicine (Tokyo, Japan), 61, 14, 2187, 2190, 15 Jul. 2022, [Domestic magazines]
  English, Scientific journal, Lumbar hernias are extremely rare. The posterolateral abdominal wall has two susceptible areas - the superior (Grynfeltt-Lesshaft's triangle) and the inferior (Petit's triangle) lumbar triangles - that cause superior and inferior lumbar hernias, respectively. We herein report a 67-year-old woman with nephrotic-range proteinuria caused by primary superior lumbar hernia. Superior lumbar hernias should be considered as a differential disease causing massive orthostatic proteinuria in adults. The present case highlights the importance of considering lumbar hernia in patients with flank swelling and the potential complications that may result from a missed diagnosis.
• Jaccoud's arthropathy in the elderly.
  Taro Horino; Hiroaki Ueba; Satoshi Inotani; Masashi Izumi; Osamu Ichii; Yoshio Terada
  Rheumatology (Oxford, England), 61, 7, e212, 06 Jul. 2022, [International Magazine]
  English, Scientific journal
• Haematologic malignancy-associated mucocutaneous paraneoplastic syndrome.
  Taro Horino; Satoko Ohmi; Satoshi Inotani; Hideki Nakajima; Hiroshi Ohnishi; Masahiro Komori; Osamu Ichii; Yoshio Terada
  Rheumatology (Oxford, England), 61, 7, e207-e208, 06 Jul. 2022, [International Magazine]
  English, Scientific journal
• Turkeys possess diverse Siaα2-3Gal glycans that facilitate their dual susceptibility to avian influenza viruses isolated from ducks and chickens.
  Daiki Kobayashi; Takahiro Hiono; Osamu Ichii; Shoko Nishihara; Sayaka Takase-Yoden; Kazuo Yamamoto; Hiroto Kawashima; Norikazu Isoda; Yoshihiro Sakoda
  Virus research, 315, 198771, 198771, 02 Jul. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Genital organ-associated lymphoid tissues arranged in a ring in the mucosa of cow vaginal vestibules.
  Tsolmon Chuluunbaatar; Osamu Ichii; Md Abdul Masum; Takashi Namba; Md Rashedul Islam; Yuki Otani; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Research in veterinary science, 145, 147, 158, Jul. 2022, [International Magazine]
  English, Scientific journal, Female reproductive tracts are equipped with local and mucosal immune systems; however, structural information remains unclear for farm animals. In this study, the mucosa-associated lymphoid tissue-like structures in cow reproductive tracts were described. Vaginal vestibule (VV) and external parts of the genital organ, including the clitoris and vulva, were morphologically analyzed. Whole-mount specimens revealed several hematoxylin-positive spots arranged in a ring in the mucosa. Histologically, these spots were aggregated immune cells and defined as genital organ-associated lymphoid tissues (GOALTs). GOALTs were composed of lymphatic follicles (LFs) or diffuse lymphoid tissues (DLTs) at different depths of lamina propria. LFs frequently contained germinal centers. Scattered lymphocytes occupied the border area between follicles and epithelium, whereas DLTs had indefinite shapes. GOALTs contained immune cells and high endothelial venules. B cells were dominant both in LFs and DLTs. Abundant collagenous fibers were stretched across VV lamina propria, whereas reticular fibers were primarily observed in the DLT rather than LF. The epithelium covering of GOALTs was partially or fully disrupted by the invasion of immune cells toward the VV lumen. These findings suggest GOALTs function as a "genital lymphoid ring" as in Waldeyer's pharyngeal ring and act as immunological gate systems in cow reproductive tracts.
• Modified foreign body reaction to silicone imbedded in subcutaneous tissues by different mouse systemic immune conditions.
  Tomohiro Yamakawa; Osamu Ichii; Teppei Nakamura; Takashi Namba; Yaser Hosny Ali Elewa; Md Abdul Masum; Yuki Otani; Takanori Nishimura; Yasuhiro Kon
  Journal of biomedical materials research. Part A, 110, 12, 1921, 1931, 30 Jun. 2022, [International Magazine]
  English, Scientific journal, Foreign body reaction (FBR) causes unexpected adverse effects due to implanted materials in humans and animals. Inflammation and subsequent fibrosis during FBR seems to be affected by recipient immunity, such as the balance of T helper (Th) response that has the potential to regulate FBR-related macrophage function. Here, the immunological effects of FBR on subcutaneously imbedded silicone tubes (ST) at 8 weeks were investigated histologically by comparing Th1-biased C57BL/6N, Th2-biased MRL/MpJ, and autoimmune disease-prone MRL/MpJ-Faslpr/lpr . Tissue surrounding ST (TSS) was analyzed at day (D) 7 and 14 (reaction phase) or D35 (stability phase) after surgery. In all strains, the TSS was composed of a thin layer (TL) containing fibrous tissues and loose connective tissues formed outside the TL. Few lymphocytes and mast cells, several neutrophils, and numerous macrophages infiltrated the TSS. Active vascularization was observed at D14 in all strains. For the examined indices, M1-type macrophage density in the TSS of C57BL/6N mice was significantly higher at D14 compared to other strains. No significant strain difference relating to M2-type macrophages was detected, suggesting the effects of Th1-biased immunity on FBR-related inflammation. Collagen fibers in the TSS increased in density and became stable with age in all strains. In particular, MRL/MpJ-Faslpr/lpr showed progressive fibrotic features. Serum autoantibody levels in MRL/MpJ-Faslpr/lpr mice were inversely correlated with M1-type macrophage density. These data from MRL/MpJ-Faslpr/lpr mice suggested modifications of FBR-related inflammation and fibrosis by autoimmune abnormalities. The results provide crucial insights into the pathological modification of FBR by recipient immunity and emphasize its clinicopathological importance in humans and animals.
• Clarifying expression patterns by renal lesion using transcriptome analysis and vanin-1 as a potential novel biomarker for renal injury in chickens.
  Chihiro Ishii; Yusuke K Kawai; Yoshinori Ikenaka; Naoya Maekawa; Osamu Ichii; Shouta M M Nakayama; Mayumi Ishizuka
  Poultry science, 101, 9, 102011, 102011, 16 Jun. 2022, [International Magazine]
  English, Scientific journal, Bird death is often caused by renal lesions induced by chemicals. The avian kidney has a renal portal system with significant blood flow that is sensitive to many chemicals. However, early avian biomarkers for kidney injury are yet to be identified. This study aimed to identify novel renal biomarkers. Acute kidney injury (AKI) can be divided into acute interstitial nephritis (AIN) and acute tubular necrosis (ATN). A chicken model of kidney damage was created by an injection of diclofenac or cisplatin, which caused either AIN or ATN, respectively. Microarray analysis was performed to profile the gene expression patterns in the chickens with nephropathy. A gene enrichment analysis suggested that the genes related to responses to external stimuli showed expression changes in both AIN and ATN. However, hierarchical clustering analyses suggested that gene expression patterns differed between AIN and ATN, and the number of biomarkers relating to renal damage was low. To identify early biomarkers for nephropathy, we focused on genes that were induced at various levels of renal damage. The gene, vanin-1 (VNN1) was highly induced in the early stages of renal damage. A quantitative real-time PCR analysis supported this finding. These results suggest VNN1 could be a useful early biomarker of kidney injury in avian species.
• Health impact assessment of pet cats caused by organohalogen contaminants by serum metabolomics and thyroid hormone analysis.
  Kei Nomiyama; Yasuo Yamamoto; Akifumi Eguchi; Hiroyuki Nishikawa; Hazuki Mizukawa; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Shouta M M Nakayama; Yoshinori Ikenaka; Mayumi Ishizuka
  The Science of the total environment, 842, 156490, 156490, 日本内分泌撹乱物質学会, 03 Jun. 2022, [International Magazine]
  English, Companion animals are in close contact with the human surroundings, and there is growing concern about the effects of harmful substances on the health of pet cats. In this study, we investigated the potential health effects of organohalogen compounds (OHCs) on thyroid hormone (TH) homeostasis and metabolomics in Japanese pet cats. There was a significant negative correlation between concentrations of several contaminants, such as polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), hydroxylated PCBs (OH-PCBs), hydroxylated PBDEs (OH-PBDEs), and THs in cat serum samples. These results suggested that exposure to OHCs causes a decrease in serum TH levels in pet cats. In this metabolomics study, each exposure level of parent compounds (PCBs and PBDEs) and their hydroxylated compounds (OH-PCBs and OH-PBDEs) were associated with their own unique primary metabolic pathways, suggesting that parent and phenolic compounds exhibit different mechanisms of action and biological effects. PCBs were associated with many metabolic pathways, including glutathione and purine metabolism, and the effects were replicated in in-vivo cat PCB administration studies. These results demonstrated that OHC exposure causes chronic oxidative stress in pet cats. PBDEs were positively associated with alanine, aspartate, and glutamate metabolism. Due to the chronic exposure of cats to mixtures of these contaminants, the combination of their respective metabolic pathways may have a synergistic effect.
• Anti-cyclic citrullinated peptide antibody-positive rheumatoid arthritis caused by bacterial organizing pneumonia in a patient with Sjogren's syndrome.
  Taro Horino; Mitsuharu Yoshida; Satoshi Inotani; Kazuki Anabuki; Hiroshi Ohnishi; Masahiro Komori; Osamu Ichii; Yoshio Terada
  Romanian journal of internal medicine = Revue roumaine de medecine interne, 60, 2, 127, 131, 01 Jun. 2022, [International Magazine]
  English, Scientific journal, A 58-year-old woman with a history of Sjogren's syndrome was admitted to our hospital with cough, decreased right lung breath sounds and arthralgia in both thumbs. Chest computed tomography showed consolidation with air bronchogram in the right lung. Levels of anti-cyclic citrullinated peptide antibody and rheumatoid factor levels were significantly elevated. She was diagnosed with rheumatoid arthritis induced by bacterial organizing pneumonia. Treatment with salazosulfapyridine was added for rheumatoid arthritis and arthralgia gradually improved. This case highlights that respiratory infections could lead to anti-cyclic citrullinated peptide antibody-positive rheumatoid arthritis in patients with Sjogren's syndrome.
• Histopathological Impact of Bleomycin on Lung Injury and Development of Mediastinal Fat-Associated Lymphoid Clusters in the Lymphoproliferative Mouse Model.
  Yaser Hosny Ali Elewa; Osamu Ichii; Sherif Kh A Mohamed; Yasuhiro Kon
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 1, 15, 23 May 2022, [International Magazine]
  English, Scientific journal, The purpose of this study is to elucidate the impact of bleomycin on the degree of lung injury and development of mediastinal fat-associated lymphoid clusters (MFALCs) in the lymphoproliferative mouse model (MRL/MpJ-Faslpr/lpr “Lpr”) and its control strain (MRL/MpJ “MpJ”). We analyzed immune cells, the degree of proliferation, lymphatic vessels (LVs), and high endothelial venules (HEVs) in lungs and MFALCs in Lpr and MpJ mice on the 7th and 21st days following intranasal instillation of either bleomycin (BLM group) or PBS (PBS group). The BLM group showed a significant increase in the size of MFALCs, lung injury score, and positive area ratios of LVs, HEVs, and immune cells (especially macrophages, B- and T-lymphocytes) on both days 7 and 21. Interestingly, the lungs in the BLM group on day 21 showed higher collagen deposition and cellular infiltration in MpJ and Lpr, respectively. Moreover, significant positive correlations were observed between the size of MFALCs and lung injury. In conclusion, BLM could exert lung fibrosis or lymphoproliferative infiltration in chronic stages in MpJ and Lpr, respectively, and this varied effect could be due to the variations in the degree of immune cell proliferation and the development of LVs and HEVs among the studied strains.
• Scanning electron microscopy of Quilonia renniei from Asian elephants revealing variation in coronal leaflet number.
  Hla Myet Chel; Saw Bawm; Lat Lat Htun; Md Abdul Masum; Osamu Ichii; Nariaki Nonaka; Ryo Nakao; Ken Katakura
  Parasitology, 149, 4, 529, 533, Apr. 2022, [International Magazine]
  English, Scientific journal, Although parasitic nematodes in the genera Murshidia and Quilonia (family Strongylidae) are recognized as major gastrointestinal parasites in Asian elephants, they have been poorly studied. Recently, light micrographs of these parasites in Myanmar have been presented, almost 100 years after the original drawings. However, the number of coronal leaflets, a key taxonomic feature of Quilonia species, has not been precisely determined based on light microscopy. The current study aimed to determine the exact number of coronal leaflets in Quilonia renniei specimens from Asian elephants in Myanmar. On the basis of scanning electron micrographs, leaflet number in females (19–20, average 19.7, n = 9) was significantly higher (P < 0.005) than that in males (16–19, average 18.1, n = 8). This compares with 18 coronal leaflets indicated in the original species description. Specimens bearing 19 coronal leaflets were most numerous, followed by those with 20 leaflets. Median-joining network analysis of mitochondrial cytochrome c oxidase subunit I gene sequences with 16 haplotypes from 19 individuals revealed no clear association between parasite populations and the number of coronal leaflets. These results highlight the importance of determining the number of coronal leaflets in the taxonomy of Q. renniei and other related Quilonia species infecting Asian elephants.
• Immunohistochemical Expression of TGF-β1 in Kidneys of Cats with Chronic Kidney Disease.
  Yuki Uehara; Yu Furusawa; Md Shafiqul Islam; Osamu Yamato; Hitoshi Hatai; Osamu Ichii; Akira Yabuki
  Veterinary sciences, 9, 3, 114, 114, MDPI AG, 03 Mar. 2022, [International Magazine]
  English, Scientific journal, Transforming growth factor-beta 1 (TGF-β1) plays a central role in the progression of chronic kidney disease (CKD). However, in feline CKD, renal expression of TGF-β1 and how it changes as the disease progresses have not been fully studied. In the present study, we immunohistochemically assessed the renal expression levels of TGF-β1 in cats with CKD and statistically analyzed its correlation with CKD severity. Clear immunosignals were detected in the glomerular mesangial cells, Bowman’s capsules, proximal tubules, distal nephrons, platelets, and vascular smooth muscles in the kidneys of cats with CKD. Statistically, luminal signals in the distal nephrons showed positive correlations with plasma creatinine levels and glomerulosclerosis, while those in the proximal tubules and platelets showed negative correlations with plasma urea and/or creatinine levels. Therefore, it was suggested that the changes in the renal expression of TGF-β1 could be associated with progression of feline CKD.
• In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens.
  Takuma Ariizumi; Shiro Murata; Sotaro Fujisawa; Masayoshi Isezaki; Takumi Sato; Eiji Oishi; Akira Taneno; Osamu Ichii; Naoya Maekawa; Tomohiro Okagawa; Satoru Konnai; Kazuhiko Ohashi
  Poultry science, 101, 3, 101638, 101638, Elsevier BV, Mar. 2022, [International Magazine]
  English, Scientific journal, Poultry red mites (PRMs, Dermanyssus gallinae) are hematophagous ectoparasites that negatively affect egg production, which causes serious economic losses to the poultry industry worldwide. Currently, the emergence of acaricide-resistant PRMs has impeded PRM control in poultry farms. Several alternatives for acaricide use have been described for managing PRM-caused problems. Vaccination is among the methods for controlling PRMs in poultry houses. Currently, several candidates for vaccine antigens have been identified. This study identified a cysteine protease, Deg-CPR-2, which differs from 2 other previously reported cysteine proteases in PRMs, from previously obtained data from RNA-sequencing (RNA-seq) analysis. We investigated the characteristics of Deg-CPR-2 and assessed its efficacy as a vaccine antigen in vitro. Phylogenetic analysis revealed that Deg-CPR-2 belonged to a different cluster from those of other cysteine proteases in PRMs. This cluster also included cathepsin L-like proteases, enzymes thought to be involved in hemoglobin digestion in ticks. Expression analysis revealed Deg-CPR-2 expression in midguts and all the life-stages; however, there were differences in the expression levels across the life-stages. The enzyme activity of recombinant Deg-CPR-2 was inhibited in the presence of a cysteine protease inhibitor, which suggests that Deg-CPR-2 functions as a cysteine protease in PRMs. Finally, there was an in vitro increase in the mortality of PRMs, mainly protonymphs that were artificially fed with plasma from chickens immunized with Deg-CPR-2. These findings suggest that Deg-CPR-2 may contribute to protein digestion in the midgut of PRMs and is crucially involved in physiological processes in PRMs. Additionally, immunization with Deg-CPR-2 may reduce the number of protonymphs, and Deg-CPR-2 should be considered as a candidate antigen for anti-PRM vaccine development.
• Cotton rats (Sigmodon hispidus) with a high prevalence of hydrocephalus without clinical symptoms.
  Daisuke Kondoh; Teppei Nakamura; Erika Tsuji; Marina Hosotani; Osamu Ichii; Takao Irie; Takashi Mishima; Ken-Ichi Nagasaki; Yasuhiro Kon
  Neuropathology : official journal of the Japanese Society of Neuropathology, 42, 1, 16, 27, Wiley, Feb. 2022, [International Magazine]
  English, Scientific journal, Normal-pressure hydrocephalus (NPH) is a condition in which the ventricle is enlarged without elevated cerebrospinal fluid pressure, and it generally develops in later life and progresses slowly. A complete animal model that mimics human idiopathic NPH has not yet been established, and the onset mechanisms and detailed pathomechanisms of NPH are not fully understood. Here, we demonstrate a high spontaneous prevalence (34.6%) of hydrocephalus without clinical symptoms in inbred cotton rats (Sigmodon hispidus). In all 46 hydrocephalic cotton rats, the severity was mild or moderate and not severe. The dilation was limited to the lateral ventricles, and none of the hemorrhage, ventriculitis, meningitis, or tumor formation was found in hydrocephalic cotton rats. These findings indicate that the type of hydrocephalus in cotton rats is similar to that of communicating idiopathic NPH. Histopathological examinations revealed that the inner granular and pyramidal layers (layers IV and V) of the neocortex became thinner in hydrocephalic brains. A small number of pyramidal cells were positive for Fluoro-Jade C (a degenerating neuron marker) and ionized calcium-binding adaptor molecule 1 (Iba1)-immunoreactive microglia were in contact with the degenerating neurons in the hydrocephalic neocortex, suggesting that hydrocephalic cotton rats are more or less impaired projections from the neocortex. This study highlights cotton rats as a candidate for novel models to elucidate the pathomechanism of idiopathic NPH. Additionally, cotton rats have some noticeable systemic pathological phenotypes, such as chronic kidney disease and metabolic disorders. Thus, this model might also be useful for researching the comorbidities of NPH to other diseases.
• Newly diagnosed IgA nephropathy with gross haematuria following COVID-19 vaccination.
  T Horino; D Sawamura; S Inotani; M Ishihara; M Komori; O Ichii
  QJM : monthly journal of the Association of Physicians, 115, 1, 28, 29, 21 Jan. 2022, [International Magazine]
  English, Scientific journal
• Membranous Nephropathy Associated With Multicentric Castleman Disease-Efficacy of Interleukin 6 Antibody for Nephrotic Syndrome.
  Taro Horino; Takeshi Kashio; Satoshi Inotani; Masayuki Ishihara; Osamu Ichii
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 28, 1, e1-e2, 01 Jan. 2022, [International Magazine]
  English, Scientific journal
• Pseudoxanthomatous rheumatoid nodule
  Taro Horino; Satoshi Inotani; Hideki Nakajima; Hiroshi Ohnishi; Masahiro Komori; Osamu Ichii; Yoshio Terada
  The Lancet Rheumatology, 5, 2, e110, Elsevier BV, Jan. 2022, [International Magazine]
  English, Scientific journal
• Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen.
  Sotaro Fujisawa; Shiro Murata; Masayoshi Isezaki; Takuma Ariizumi; Takumi Sato; Eiji Oishi; Akira Taneno; Naoya Maekawa; Tomohiro Okagawa; Osamu Ichii; Satoru Konnai; Kazuhiko Ohashi
  Parasitology, 149, 1, 105, 115, Jan. 2022, [International Magazine]
  English, Scientific journal
• Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens.
  Sotaro Fujisawa; Shiro Murata; Masayoshi Isezaki; Takuma Ariizumi; Takumi Sato; Eiji Oishi; Akira Taneno; Naoya Maekawa; Tomohiro Okagawa; Osamu Ichii; Satoru Konnai; Kazuhiko Ohashi
  Vaccines, 9, 12, 1472, 1472, MDPI AG, 13 Dec. 2021, [International Magazine]
  English, Scientific journal, Poultry red mite (PRM; Dermanyssus gallinae) is a hazardous, blood-sucking ectoparasite of birds that constitutes a threat to poultry farming worldwide. Acaricides, commonly used in poultry farms to prevent PRMs, are not effective because of the rapid emergence of acaricide-resistant PRMs. However, vaccination may be a promising strategy to control PRM. We identified a novel cystatin-like molecule in PRMs: Dg-Cys. Dg-Cys mRNA expression was detected in the midgut and ovaries, in all stages of life. The PRM nymphs that were artificially fed with the plasma from chickens that were immunized with Dg-Cys in vitro had a significantly reduced reproductive capacity and survival rate. Moreover, combination of Dg-Cys with other antigen candidates, like copper transporter 1 or adipocyte plasma membrane-associated protein, enhanced vaccine efficacies. vaccination and its application as an antigen for cocktail vaccines could be an effective strategy to reduce the damage caused by PRMs in poultry farming.
• Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2.
  Marumi Ohno; Michihito Sasaki; Yasuko Orba; Toshiki Sekiya; Md Abdul Masum; Osamu Ichii; Tatsuya Sawamura; Akemi Kakino; Yasuhiko Suzuki; Hiroshi Kida; Hirofumi Sawa; Masashi Shingai
  Viruses, 13, 11, 22 Oct. 2021, [International Magazine]
  English, Scientific journal
• Close Association Between Altered Urine-urothelium Barrier and Tertiary Lymphoid Structure Formation in the Renal Pelvis During Nephritis.
  Osamu Ichii; Marina Hosotani; Md Abdul Masum; Taro Horino; Teppei Nakamura; Takashi Namba; Yuki Otani; Yaser Elewa; Yasuhiro Kon
  Journal of the American Society of Nephrology : JASN, 33, 1, 88, 107, 22 Oct. 2021, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, Background: Kidneys with chronic inflammation develop tertiary lymphoid structures (TLSs). Infectious pyelonephritis is characterized by renal pelvis (RP) inflammation. However, the pathological features of TLSs, including their formation and association with non-infectious nephritis, are unclear. Methods: RPs from humans and mice that were healthy or had non-infectious chronic nephritis, were analyzed for TLS development, and the mechanism of TLS formation investigated using urothelium or lymphoid structure cultures. Results: Regardless of infection, TLSs in the RP, termed urinary tract-associated lymphoid structures (UTALSs), formed in humans and mice with chronic nephritis. Moreover, urine played a unique role in UTALS formation. Specifically, we identified urinary IFN-γ as a candidate factor affecting urothelial barrier integrity because it alters occludin expression. In a nephritis mouse model, urine leaked from the lumen of the RP into the parenchyma. In addition, urine immunologically stimulated UTALS-forming cells via cytokine (IFN-γ, TNF-α) and chemokine (CXCL9, CXCL13) production. CXCL9 and CXCL13 were expressed in UTALS stromal cells and urine stimulation specifically induced CXCL13 in cultured fibroblasts. Characteristically, type XVII collagen (BP180), a candidate autoantigen of bullous pemphigoid, was ectopically localized in the urothelium covering UTALSs and associated with UTALS development by stimulating CXCL9 or IL-22 induction via the TNF-α/FOS/JUN pathway. Notably, UTALS development indices were positively correlated with chronic nephritis development. Conclusion: TLS formation in the RP is possible and altered urine-urothelium barrier-basedUTALS formation may represent a novel mechanism underlying the pathogenesis of chronic nephritis, regardless of urinary tract infection.
• Necrotizing granulomatous vasculitis without crescentic glomerulonephritis in granulomatosis with polyangiitis.
  Taro Horino; Satoshi Inotani; Osamu Ichii; Yoshio Terada
  QJM : monthly journal of the Association of Physicians, 115, 2, 98, 99, 20 Oct. 2021, [International Magazine]
  English, Scientific journal
• REV-ERB agonist suppresses IL-17 production in γδT cells and improves psoriatic dermatitis in a mouse model.
  Shangyi Wang; Mina Kozai; Hironobu Mita; Zimeng Cai; Md Abdul Masum; Osamu Ichii; Kensuke Takada; Mutsumi Inaba
  Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 144, 112283, 112283, Elsevier BV, 07 Oct. 2021, [International Magazine]
  English, Psoriasis is a chronic inflammatory skin disease characterized by epidermal hyperplasia and cellular infiltration. Studies have shown that disease development depends on proinflammatory cytokines, such as interleukin (IL)-23 and IL-17. It has been suggested that IL-23 produced by innate immune cells, such as macrophages, stimulates a subset of helper T cells to release IL-17, promoting neutrophil recruitment and keratinocyte proliferation. However, recent studies have revealed the crucial role of γδT cells in psoriasis pathogenesis as the primary source of dermal IL-17. The nuclear receptors REV-ERBs are ligand-dependent transcription factors recognized as circadian rhythm regulators. REV-ERBs negatively regulate IL-17-producing helper T cells, whereas the involvement of REV-ERBs in regulating IL-17-producing γδT (γδT17) cells remains unclear. Here we revealed the regulatory mechanism involving γδT17 cells through REV-ERBs. γδT17 cell levels were remarkably elevated in the secondary lymphoid organs of mice that lacked an isoform of REV-ERBs. A synthetic REV-ERB agonist, SR9009, suppressed γδT17 cells in vitro and in vivo. Topical application of SR9009 to the skin reduced the inflammatory symptoms of psoriasiform dermatitis in mice. The results of this study provide a novel therapeutic approach for psoriasis targeting REV-ERBs in γδT17 cells.
• Sulfated glycans containing NeuAcα2-3Gal facilitate the propagation of human H1N1 influenza A viruses in eggs.
  Tomomi Ichimiya; Masatoshi Okamatsu; Takaaki Kinoshita; Daiki Kobayashi; Osamu Ichii; Naoki Yamamoto; Yoshihiro Sakoda; Hiroshi Kida; Hiroto Kawashima; Kazuo Yamamoto; Sayaka Takase-Yoden; Shoko Nishihara
  Virology, 562, 29, 39, Oct. 2021, [International Magazine]
  English, Scientific journal, When human influenza viruses are isolated and passaged in chicken embryos, variants with amino acid substitutions around the receptor binding site of hemagglutinin (HA) are selected; however, the mechanisms that underlie this phenomenon have yet to be elucidated. Here, we analyzed the receptor structures that contributed to propagation of egg-passaged human H1N1 viruses. The analysis included seasonal and 2009 pandemic strains, both of which have amino acid substitutions of HA found in strains isolated or passaged in eggs. These viruses exhibited high binding to sulfated glycans containing NeuAcα2-3Gal. In MDCK cells overexpressing the sulfotransferase that synthesize Galβ1-4(SO3--6)GlcNAc, production of human H1N1 viruses was increased up to 90-fold. Furthermore, these sulfated glycans were expressed on the allantoic and amniotic membranes of chicken embryos. These results suggest that 6-sulfo sialyl Lewis X and/or NeuAcα2-3Galβ1-4(SO3--6)GlcNAc are involved in efficient propagation of human H1N1 viruses in chicken embryos.
• Compartmentalization of interleukin 36 subfamily according to inducible and constitutive expression in the kidneys of a murine autoimmune nephritis model.
  Takashi Namba; Osamu Ichii; Teppei Nakamura; Md Abdul Masum; Yuki Otani; Marina Hosotani; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Cell and tissue research, 386, 1, 59, 77, Oct. 2021, [International Magazine]
  English, Scientific journal, The interleukin (IL) 36 subfamily belongs to the IL-1 family and is comprised of agonists (IL-36α, IL-36β, IL-36γ) and antagonists (IL-36Ra, IL-38). We previously reported IL-36α overexpression in renal tubules of chronic nephritis mice. To understand the localization status and biological relationships among each member of the IL-36 subfamily in the kidneys, MRL/MpJ-Faslpr/lpr mice were investigated as autoimmune nephritis models using pathology-based techniques. MRL/MpJ-Faslpr/lpr mice exhibited disease onset from 3 months and severe nephritis at 6-7 months (early and late stages, respectively). Briefly, IL-36γ and IL-36Ra were constitutively expressed in murine kidneys, while the expression of IL-36α, IL-36β, IL-36Ra, and IL-38 was induced in MRL/MpJ-Faslpr/lpr mice. IL-36α expression was significantly increased and localized to injured tubular epithelial cells (TECs). CD44+-activated parietal epithelial cells (PECs) also exhibited higher IL-36α-positive rates, particularly in males. IL-36β and IL-38 are expressed in interstitial plasma cells. Quantitative indices for IL-36α and IL-38 positively correlated with nephritis severity. Similar to IL-36α, IL-36Ra localized to TECs and PECs at the late stage; however, MRL/MpJ-Faslpr/lpr and healthy MRL/MpJ mice possessed IL-36Ra+ smooth muscle cells in kidney arterial tunica media at both stages. IL-36γ was constitutively expressed in renal sympathetic axons regardless of strain and stage. IL-36 receptor gene was ubiquitously expressed in the kidneys and was induced proportional to disease severity. MRL/MpJ-Faslpr/lpr mice kidneys possessed significantly upregulated IL-36 downstream candidates, including NF-κB- or MAPK-pathway organizing molecules. Thus, the IL-36 subfamily contributes to homeostasis and inflammation in the kidneys, and especially, an IL-36α-dominant imbalance could strongly impact nephritis deterioration.
• In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens.
  Sotaro Fujisawa; Shiro Murata; Masaki Takehara; Julia Aoyama; Ayu Morita; Masayoshi Isezaki; Shwe Yee Win; Takuma Ariizumi; Takumi Sato; Eiji Oishi; Akira Taneno; Naoya Maekawa; Tomohiro Okagawa; Osamu Ichii; Satoru Konnai; Kazuhiko Ohashi
  Vaccine, 39, 41, 6057, 6066, 01 Oct. 2021, [International Magazine]
  English, Scientific journal, The poultry red mite (Dermanyssus gallinae; PRM) is a blood-sucking ectoparasite of chickens that is a threat to poultry farming worldwide and significantly reduces productivity in the egg-laying industry. Chemical acaricides that are widely used in poultry farms for the prevention of PRMs are frequently ineffective due to the emergence of acaricide-resistant PRMs. Therefore, alternative control methods are needed, and vaccination is a promising strategy for controlling PRMs. A novel adipocyte-plasma membrane-associated protein-like molecule (Dg-APMAP) is highly expressed in blood-fed PRMs according to a previous RNA sequencing analysis. Here, we attempted to identify the full sequence of Dg-APMAP, study its expression in different life stages of PRMs, and evaluate its potential as a vaccine antigen. Dg-APMAP mRNA was expressed in the midgut and ovaries, and in all life stages regardless of feeding states. Importantly, in vitro feeding of PRMs with plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-APMAP significantly reduced their survival rate in nymphs and adults, which require blood meals. Our data suggest that the host immune responses induced by vaccination with Dg-APMAP could be an effective strategy to reduce the suffering caused by PRMs in the poultry industry.
• Crescentic glomerulonephritis induced by anti-vascular endothelial growth factor receptor 2 antibody.
  Taro Horino; Satoshi Inotani; Tsunehiro Ochi; Masayuki Ishihara; Kenichi Yagyu; Osamu Ichii; Kazushige Uchida; Mikiya Fujieda; Yoshio Terada
  Nephrology (Carlton, Vic.), 27, 4, 384, 385, 07 Sep. 2021, [International Magazine]
  English
• 自己免疫疾患マウスモデルにおける自己免疫性肺傷害とmediastinal fat-associated lymphoid clusters形成に対するデキサメタゾンの改善作用(Ameliorative effect of dexamethasone on the development of autoimmune lung injury and mediastinal fat-associated lymphoid clusters in autoimmune disease mouse model)
  Elewa Yaser Hosny Ali; Masum Md. Abdul; 市居 修; 中村 鉄平; Sherif Khairy; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 164回, 8, [AO, 22], (公社)日本獣医学会, Sep. 2021, [International Magazine]
  English, Scientific journal, In our previous study, we revealed the ameliorative therapeutic effect of dexamethasone (Dex) for Lupus nephritis lesions in the MRL/MpJ-Fas lpr/lpr (Lpr) mouse model. The female Lpr mice developed a greater number of mediastinal fat-associated lymphoid clusters (MFALCs) and inflammatory lung lesions compared to the male mice. However, the effect of Dex, an immunosuppressive drug, on both lung lesions and the development of MFALCs in Lpr mice has not been identified yet. Therefore, in this study, we compared the development of lung lesions and MFALCs in female Lpr mice that received either saline (saline group "SG") or dexamethasone (dexamethasone group "DG") in drinking water as a daily dose along with weekly intraperitoneal injections for 10 weeks. Compared to the SG group, the DG group showed a significant reduction in the levels of serum anti-dsDNA antibodies, the size of MFALCs, the degree of lung injury, the area of high endothelial venules (HEVs), and the number of proliferating and immune cells in both MFALCs and the lungs. A significant positive correlation was observed between the size of MFALCs and the cellular aggregation in the lungs of Lpr mice. Therefore, this study confirmed the ameliorative effect of Dex on the development of lung injury and MFALCs via their regressive effect on both immune cells' proliferative activity and the development of HEVs. Furthermore, the reprogramming of MFALCs by targeting immune cells and HEVs may provide a therapeutic strategy for autoimmune-disease-associated lung injury.
• Novel polychrome staining distinguishing osteochondral tissue and bone cells in decalcified paraffin sections.
  Teppei Nakamura; Kanako Sumi; Erika Tsuji; Marina Hosotani; Takashi Namba; Osamu Ichii; Takao Irie; Ken-Ichi Nagasaki; Yasuhiro Kon; Takashi Mishima; Tomoji Yoshiyasu
  Cell and tissue research, 385, 3, 727, 737, Sep. 2021, [International Magazine]
  English, Scientific journal, The bone is a dynamic and metabolically active organ in which growth and resorption of the osteochondral matrix is orchestrated by osteoblasts and osteoclasts. For decalcified paraffin-embedded specimens, decalcifying agents alter the staining intensity, and excess decalcification interferes with bone staining. Robust bone staining methods independent of the decalcification conditions and animal species are lacking. In this study, we have developed a novel polychrome staining method, named JFRL staining, which stains the components of osteochondral tissue in different colors. With this staining we could visualize the hyaline cartilage as blue by alcian blue, osteoid as red by picrosirius red, and mineralized bone as green by picro-light green SF or picro-naphthol green B and easily distinguished osteoblasts, osteocytes, and osteoclasts. In mineralized bone, this staining revealed the obvious lamellar structures and woven bone. Notably, this staining was independent of the decalcification conditions and experimental animal species examined. To verify the usefulness of JFRL staining, we observed cotton rat tail which has shorter length and shows a false autotomy. The caudal vertebrae were normally developed via endochondral ossification without a fracture plane. At 6 months of age, the number of chondrocytes declined and the hypertrophic zone was absent at the epiphyseal plate, which might reflect the shorter tail. In conclusion, JFRL staining is the first method to simultaneously distinguish osteochondral matrix and bone cells in one section regardless of decalcifying conditions. This robust staining will provide new information for a wide number of biomedical fields, including bone development, physiology, and pathology.
• Morphological Features of the Testis among Autoimmune Mouse Model and Healthy Strains.
  Zeinab Shouman; Hany E Marei; Ahmed Abd-Elmaksoud; Mohamed Kassab; Takashi Namba; Md Abdul Masum; Yasser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 1, 9, 05 Aug. 2021, [International Magazine]
  English, Scientific journal, Autoimmune diseases play a critical role in the progression of infertility in both sexes and their severity has been reported to increase with age. However, few reports have discussed their effect on the morphological features of the testis. Therefore, we compared the morphological alterations in the testes of autoimmune model mice (MRL/MpJ-Faslpr) and the control strain (MRL/MpJ) with those of their background strain (C57BL/6N) at 3 and 6 months. Furthermore, we analyzed the changes in spermatocytes, Sertoli cells, immune cells, and Zonula occludens-1 junctional protein by immunohistochemical staining. The MRL/MpJ-Faslpr mice showed a significant increase in the serum Anti-double stranded DNA antibody level, relative spleen weight, and seminiferous luminal area when compared with other studied two strains. In contrast, a significant decrease in the relative testis weight, and numbers of both Sertoli, meiotic spermatocyte was observed in MRL/MpJ-Faslpr and MRL/MpJ mice compared with C57BL/6N mice especially at 6 months. Similarly, Zonula occludens-1 junctional protein positive cells showed a significant decrease in the same strains at 6 months. However, no immune cell infiltration could be observed among the studied three strains. Our findings suggest that the increase in autoimmune severity especially with age could lead to infertility through loss of spermatogenic and Sertoli cells, rather than the disturbance of the blood-testis barrier.
• Altered Renal Pathology in an Autoimmune Disease Mouse Model After Induction of Diabetes Mellitus.
  Shiori Hiramatsu; Osamu Ichii; Takashi Namba; Yuki Otani; Teppei Nakamura; Md Abdul Masum; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 27, 4, 897, 909, Aug. 2021, [International Magazine]
  English, Scientific journal, Diabetes mellitus (DM) is a predisposing factor for renal disorder progression and is referred to as diabetic kidney disease (DKD). However, there are no reports of DKD with an underlying autoimmune disorder. In this study, we compared the pathophysiological changes caused by DM induction after streptozotocin (STZ) injection in comparison with that in a control group receiving citrate buffer (CB) in the autoimmune disease model mice “BXSB/MpJ-Yaa” (Yaa) and the wild-type strain BXSB/MpJ. Both strains showed hyperglycemia after 12 weeks of STZ injection. Interestingly, the Yaa group developed membranous and proliferative glomerulonephritis, which tended to be milder glomerular lesions in the STZ group than in the CB group, as indicated by a decreased mesangial area and ameliorated albuminuria. Statistically, the indices for hyperglycemia and autoimmune abnormalities were negatively and positively correlated with the histopathological parameters for mesangial matrix production and glomerular proliferative lesions, respectively. STZ treatment induced renal tubular anisonucleosis and dilations in both strains, and they were more severe in Yaa. Significantly decreased cellular infiltration was observed in the Yaa group compared to the CB group. Thus, in DKD related to autoimmune nephritis, hyperglycemia modifies its pathology by decreasing the mesangial area and interstitial inflammation and aggravating renal tubular injury.
• Anti-Ku Antibody-Related Scleroderma-Polymyositis Overlap Syndrome Associated With Hypothyroid Myopathy.
  Yuji Kadowaki; Taro Horino; Takeshi Kashio; Hirofumi Nishikawa; Satoshi Inotani; Tatsuki Matsumoto; Masayuki Ishihara; Osamu Ichii
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 27, 5, e200-e201, 01 Aug. 2021, [International Magazine]
  English, Scientific journal
• Lupus-related protein-losing enteropathy associated with pseudo-pseudo Meigs' syndrome and successfully treated with hydroxychloroquine.
  Taro Horino; Masami Ogasawara; Takeshi Kashio; Satoshi Inotani; Masayuki Ishihara; Hiroshi Ohnishi; Masahiro Komori; Osamu Ichii; Yoshio Terada
  Romanian journal of internal medicine = Revue roumaine de medecine interne, 60, 1, 85, 89, 31 Jul. 2021, [International Magazine]
  English, Scientific journal, We herein report the first case of lupus-related protein-losing enteropathy associated with pseudo-pseudo Meigs' syndrome. Lupus-related protein-losing enteropathy and pseudo-pseudo Meigs' syndrome are extremely rare complications in patients with systemic lupus erythematosus, Both have a similar clinical course characterized by producing marked ascites, and respond to steroids in typical cases. However, in our case, steroid monotherapy was inadequate and the addition of hydroxychloroquine was effective for their treatment. Furthermore, no reports have previously confirmed elevated CA 125 levels with lupus-related protein-losing enteropathy or increased 99mTc-HSA activity with pseudo-pseudo Meigs' syndrome. In addition, we are the first to report an evaluation of the histopathology of lupus-related protein-losing enteropathy. Previously reported cases have been described as being caused by either pseudo-Meigs's syndrome or lupus-related protein-losing enteropathy as the cause of the rare pathology that causes marked pleural effusion and ascites in patients with systemic lupus erythematosus, but It has not been evaluated whether the other is co-occurring. Our case highlights that there is a potential case of overlapping lupus-related protein-losing enteropathy and pseudo-Pseudo-Meigs's syndrome. Furthermore, it is possible that patients with marked ascites with elevated CA 125 levels were mistakenly diagnosed with Meigs's syndrome or pseudo-Meigs's syndrome associated with malignant or benign ovarian tumors and underwent surgery. Clinicians should not forget SLE with pseudo-Pseudo-Meigs's syndrome as one of the differential diagnoses for marked ascites with elevated CA 125 levels.
• Spatiotemporal histological changes observed in mouse subcutaneous tissues during the foreign body reaction to silicone.
  Sao Oe; Md Abdul Masum; Osamu Ichii; Takanori Nishimura; Teppei Nakamura; Takashi Namba; Yuki Otani; Yasuhide Nakayama; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Journal of biomedical materials research. Part A, 109, 7, 1220, 1231, Jul. 2021, [International Magazine]
  English, Scientific journal, We investigated spatiotemporal changes in host tissues during foreign body reactions. Silicone tube was subcutaneously embedded into ICR mice, and tissue surrounding silicone (TSS) was observed at 2, 7, 14, 21, 28, 43, and 70 days (D) postsurgery. The thin layer (TL) and loose connective tissues (LCTs) (inside and outside the TSS) developed until D21 and densified afterward. Neutrophils infiltrated the TSS until D14 and formed neutrophil extracellular traps (NETs) in the TL during D7-21. In the LCTs, mast cell counts increased until D21, and macrophage numbers peaked at D14. Several macrophages showed LYVE-1 expression, supporting a tissue-remodeling role. Developmental indices of collagen fibers (CFs) and reticular fibers (RFs) increased during D2-21. NETs, but not neutrophils, were detected after D28. Mast cell numbers peaked at D43 and were maintained until D70. Myofibroblasts consistently localized to the TL from D14. During D21-28, the area of connective tissue (CNT), and CFs and RFs decreased and increased, respectively, and both remained constant during D28-70. The CF density remained constant from D21 and increased at D70. Thus, TSS showed two phases: inflammation and CNT development (D2-21), and inflammation convergence and CNT stabilization (D28-70). These results provide insights into foreign body reactions in clinical cases.
• Possible Crosstalk of the Immune Cells within the Lung and Mediastinal Fat-Associated Lymphoid Clusters in the Acute Inflammatory Lung Asthma-Like Mouse Model.
  Yaser Hosny Ali Elewa; Mahmoud Mansour Abd Elwakil; Osamu Ichii; Teppei Nakamura; Sherif Kh A Mohamed; Yasuhiro Kon
  International journal of molecular sciences, 22, 13, 26 Jun. 2021, [International Magazine]
  English, Scientific journal, Recently, we clarified the function of mediastinal fat-associated lymphoid clusters (MFALCs) in the progression of several respiratory diseases. However, their role has not yet been identified in the lung asthmatic condition. Hence, we compared the immune cells in lung and MFALCs of C57BL/6N mice on days 3 and 7 following intranasal instillation of either papain (papain group "PG") or phosphate buffer saline (PBS) (vehicle group "VG"). The PG showed significantly prominent MFALCs, numerous goblet cells (GCs), and higher index ratios of different immune cells (macrophages, natural helper cells (NHC), B- and T-lymphocytes) within the MFALCs and lung than in the VG on both days 3 and 7. Interestingly, a tendency of decreased size of MFALCs and a significant reduction in the number of GCs and immune cells were observed within the MFALCs and lung in the PG on day 7 than on day 3. Furthermore, the quantitative parameters of these immune cells in MFALCs were significantly and positively correlated with the size of MFALCs and immune cells in the lung. This suggested that the possible crosstalk between immune cells within MFALCs and the lung could play a critical role in the progression and recovery of the acute inflammatory lung asthma.
• Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (Sigmodon hispidus).
  Md Rashedul Islam; Osamu Ichii; Teppei Nakamura; Takao Irie; Akio Shinohara; Md Abdul Masum; Yuki Otani; Takashi Namba; Tsolmon Chuluunbaatar; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Animals : an open access journal from MDPI, 11, 6, 12 Jun. 2021, [International Magazine]
  English, Scientific journal, Most mammalian ovarian follicles contain only a single oocyte having a single nucleus. However, two or more oocytes and nuclei are observed within one follicle and one oocyte, respectively, in several species, including cotton rat (CR, Sigmodon hispidus). The present study compared ovarian histology, focusing on folliculogenesis, between two inbred CR strains, HIS/Hiph and HIS/Mz. At 4 weeks of age, ovarian sections from both the strains were analyzed histologically. Multi-oocyte follicles (MOFs) and double-nucleated oocytes (DNOs) were observed in all stages of developing follicles in HIS/Hiph, whereas HIS/Mz had MOFs up to secondary stages and lacked DNOs. The estimated total follicles in HIS/Mz were almost half that of HIS/Hiph, but interstitial cells were well developed in HIS/Mz. Furthermore, immunostaining revealed no clear strain differences in the appearance of oocytes positive for Ki67, PCNA, and p63 in MOF or DNOs; no cell death was observed in these oocytes. Ultrastructural analysis revealed more abundant mitochondrial clouds in oocytes of HIS/Hiph than HIS/Mz. Thus, we clarified the strain differences in the CR ovary. These findings indicate that early events during folliculogenesis affect the unique ovarian phenotypes found in CRs, including MOFs or DNOs, and their strain differences.
• Castrated autoimmune glomerulonephritis mouse model shows attenuated glomerular sclerosis with altered parietal epithelial cell phenotype.
  Yuki Otani; Osamu Ichii; Md Abdul Masum; Takashi Namba; Teppei Nakamura; Yasuhiro Kon
  Experimental biology and medicine (Maywood, N.J.), 246, 11, 1318, 1329, Jun. 2021, [International Magazine]
  English, Scientific journal, Sex hormones help in maintaining proper immunity as well as renal homeostasis in mammals, and these multi-functional properties characterize the onset of sex-dependent diseases. To clarify the contribution of sex hormones to autoimmune disease-related renal pathogenesis, BXSB/MpJ-Yaa was investigated as a murine autoimmune glomerulonephritis model. BXSB/MpJ-Yaa and its wild-type, BXSB/MpJ-Yaa+ were castrated or sham-operated at three weeks and examined until six months of age. Both castrated strains showed significantly lower serum testosterone levels and body weights than sham-operated mice. Castration did not change the disease phenotypes in BXSB/MpJ-Yaa+. At three months, both sham-operated and castrated BXSB/MpJ-Yaa manifested splenomegaly, autoantibody production, and glomerulonephritis, and castrated BXSB/MpJ-Yaa tended to show heavier spleen weights than the sham-operated group. At six months, both the treated BXSB/MpJ-Yaa showed equivalent autoimmune disease conditions; however, castrated mice clearly showed milder glomerular sclerotic lesions than the sham-operated groups. Urinary albumin excretion in castrated BXSB/MpJ-Yaa was significantly milder than in sham-operated mice at four months, but those of both the treated BXSB/MpJ-Yaa were comparable at six months. The examined renal histopathological indices in parietal epithelial cells were remarkably altered by castration. Briefly, castration decreased the height of parietal epithelial cells and total parietal epithelial cell number in BXSB/MpJ-Yaa at six months. For immunostaining, parietal epithelial cells facing the injured glomeruli of BXSB/MpJ-Yaa expressed CD44, an activated parietal epithelial cell marker, and CD44-positive parietal epithelial cells showed nuclear localization of the androgen receptor and proliferation marker Ki67. CD44- or Ki67-positive parietal epithelial cells were significantly fewer in castrated group than in sham-operated BXSB/MpJ-Yaa at six months. Further, quantitative indices for CD44-positive parietal epithelial cell number and frequency in renal corpuscles positively correlated with glomerular sclerotic severity in BXSB/MpJ-Yaa. In conclusion, androgen seemed to have an effect on both systemic immunity and renal morpho-function; however, the effect on the latter could be more clearly observed in BXSB/MpJ-Yaa, as parietal epithelial cell activation resulted in glomerular sclerosis.
• Immunotactoid glomerulopathy associated with monoclonal gammopathy.
  Satoshi Inotani; Taro Horino; Masayuki Ishihara; Osamu Ichii; Akinori Matsumori
  Lancet (London, England), 397, 10289, 2081, 2081, 29 May 2021, [International Magazine]
  English, Scientific journal
• Altered clinicopathology and renal pathology in dogs treated with a clinical dose of cisplatin
  Mari Okamoto; Osamu Ichii; Keiichiro Mie; Hidetaka Nishida; Hideo Akiyoshi
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 69, 2, 109, 124, May 2021
  English, Scientific journal
• Nonsteroidal Anti-Inflammatory Drug-Induced Small Bowel Stricture in Rheumatoid Arthritis.
  Taro Horino; Osamu Ichii; Tomohiro Eguchi; Satoshi Inotani; Tatsuki Matsumoto
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 27, 3, e86-e87, 01 Apr. 2021, [International Magazine]
  English, Scientific journal
• A Case of Behçet Disease Concurrent With Giant Saccular Abdominal Aortic Aneurysm.
  Taro Horino; Osamu Ichii
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 27, 3, e107-e108, 01 Apr. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Value of 18F-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography in the Diagnosis of Spondyloarthropathy-Related Aortitis.
  Taro Horino; Satoshi Inotani; Tatsuki Matsumoto; Osamu Ichii
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 27, 3, e116-e117, 01 Apr. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Unique histological features of the tail skin of cotton rat (Sigmodon hispidus) related to caudal autotomy.
  Marina Hosotani; Teppei Nakamura; Osamu Ichii; Takao Irie; Yuji Sunden; Yaser Hosny Ali Elewa; Takafumi Watanabe; Hiromi Ueda; Takashi Mishima; Yasuhiro Kon
  Biology open, 10, 2, 19 Feb. 2021, [International Magazine]
  English, Scientific journal, Caudal autotomy in rodents is an evolutionarily acquired phenomenon enabling escape from predators, by discarding the tail skin after traumatic injuries. The histological mechanisms underlying caudal autotomy seem to differ among species. Cotton rats (Sigmodon hispidus), which are important laboratory rodents for human infectious diseases, possess a fragile tail. In this study, we compared the tail histology of cotton rats with that of laboratory rats (Rattus norvegicus), which have no fragility on their tail, to elucidate the process of rodent caudal autotomy. First, the cotton rats developed a false autotomy characterized by loss of the tail sheath with the caudal vertebrae remaining without tail regeneration. Second, we found the fracture plane was continuous from the interscale of the tail epidermis to the dermis, which was lined with an alignment of E-cadherin+ cells. Third, we found an obvious cleavage plane between the dermis and subjacent tissues of the cotton-rat tail, where the subcutis was composed of looser, finer, and fragmented collagen fibers compared with those of the rat. Additionally, the cotton-rat tail was easily torn, with minimum bleeding. The median coccygeal artery of the cotton rat had a thick smooth muscle layer, and its lumen was filled with the peeled intima with fibrin coagulation, which might be associated with reduced bleeding following caudal autotomy. Taken together, we reveal the unique histological features of the tail relating to the caudal autotomy process in the cotton rat, and provide novel insights to help clarify the rodent caudal autotomy mechanism.
• Altered clinicopathology and renal pathology in dogs treated with a clinical dose of cisplatin
  Mari Okamoto; Osamu Ichii; Keiichiro Mie; Hidetaka Nishid; Hideo Akiyoshi
  Jpn J Vet Res, 69, 2, 109, 124, Feb. 2021, [Peer-reviewed], [Lead author]
  Japanese, Research institution
• Pathological Alternations of Mediastinal Fat-Associated Lymphoid Cluster and Lung in a Streptozotocin-Induced Diabetic Mouse Model.
  Yaser H A Elewa; Osamu Ichii; Teppei Nakamura; Yasuhiro Kon
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 27, 1, 187, 200, Feb. 2021, [International Magazine]
  English, Scientific journal, Diabetes is a devastating global health problem and is considered a predisposing factor for lung injury progression. Furthermore, previous reports of the authors revealed the role of mediastinal fat-associated lymphoid clusters (MFALCs) in advancing respiratory diseases. However, no reports concerning the role of MFALCs on the development of lung injury in diabetes have been published. Therefore, this study aimed to examine the correlations between diabetes and the development of MFALCs and the progression of lung injury in a streptozotocin-induced diabetic mouse model. Furthermore, immunohistochemical analysis for immune cells (CD3+ T-lymphocytes, B220+ B-lymphocytes, Iba1+ macrophages, and Gr1+ granulocytes), vessels markers (CD31+ endothelial cells and LYVE-1+ lymphatic vessels "LVs"), and inflammatory markers (TNF-α and IL-5) was performed. In comparison to the control group, the diabetic group showed lung injury development with a significant increase in MFALC size, immune cells, LVs, and inflammatory marker, and a considerable decrease of CD31+ endothelial cells in both lung and MFALCs was observed. Furthermore, the blood glucose level showed significant positive correlations with MFALCs size, lung injury, immune cells, inflammatory markers, and LYVE-1+ LVs in lungs and MFALCs. Thus, we suggest that the development of MFALCs and LVs could contribute to lung injury progression in diabetic conditions.
• Antineutrophil cytoplasmic antibody-associated vasculitis in diffuse large B cell lymphoma.
  Taro Horino; Osamu Ichii
  Nefrologia, 41, 2, 215, 216, 2021, [International Magazine]
  English
• Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus)
  Islam, M.R.; Ichii, O.; Nakamura, T.; Irie, T.; Masum, M.A.; Otani, Y.; Namba, T.; Chuluunbaatar, T.; Elewa, Y.H.A.; Kon, Y.
  Frontiers in Physiology, 11, Frontiers Media {SA}, Jan. 2021, [Peer-reviewed]
  Scientific journal
• Dual Effect of Bleomycin on Histopathological Features of Lungs and Mediastinal Fat-Associated Lymphoid Clusters in an Autoimmune Disease Mouse Model.
  Yaser Hosny Ali Elewa; Osamu Ichii; Teppei Nakamura; Yasuhiro Kon
  Frontiers in immunology, 12, 665100, 665100, 2021, [International Magazine]
  English, Scientific journal, Mediastinal fat-associated lymphoid clusters (MFALCs) are novel immune clusters that function in the pathogenesis of bleomycin (BLM)-induced pneumonitis in a C57BL/6 mouse model. However, we lack literature on the effects of BLM in an autoimmune disease mouse model (AIDM). In the present study, BLM sulfate (BLM group) or phosphate-buffered saline (PBS group) were intranasally administered in BXSB/MpJ-Yaa (Yaa) AIDM and its wild-type strains (BXSB/MpJ "BXSB") and the histopathology of MFALCs and lungs were examined on days 7 and 21 days. Immunohistochemical analysis was performed to detect lymphatic vessels (LVs), high endothelial venules (HEVs), proliferating, and immune cells. Furthermore, the mRNA expression of Yaa locus genes (TLR7, TLR8, Arhgap6, Msl3, and Tceanc) was detected in the lung tissues. Here, we show a dual effect of BLM on intra-thoracic immune hemostasis among Yaa AIDM and its corresponding wild-type strain (BXSB mice). The BLM group of BXSB mice displayed significantly higher values of lung injury scores (LIS) and size of MFALCs as compared with the corresponding PBS group. However, an opposite effect was detected in Yaa mice. Furthermore, Yaa mice displayed decreased serum autoantibody titers and downregulated expression of TLR7, TLR8, Msl3, and Tceanc in the lungs following BLM administration, especially on day 21. Interestingly, significant positive correlations were detected in both strains between the LIS and the size of MFALCs, LVs, HEVs, and proliferating cells. Conclusively, our findings revealed a crucial function of HEVs on the extent of lung injury and the development of MFALCs in BLM-administered Yaa AIDM and control BXSB mice with dual effects. Moreover, our data suggest that down regulation of Yaa locus genes could contribute as an important attributing factor leading to decrease in the degree of autoimmunity and lung injury in AIDM. Therefore, we suggest that genetic background contributes to BLM diversity among AIDM and the wild-type strain. Targeting some genes or venules could provide novel therapeutic approaches for some autoimmune-associated respiratory diseases via controlling the MFALCs development.
• Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens.
  Shiro Murata; Ayaka Taniguchi; Masayoshi Isezaki; Sotaro Fujisawa; Eishi Sakai; Akira Taneno; Osamu Ichii; Takuya Ito; Naoya Maekawa; Tomohiro Okagawa; Satoru Konnai; Kazuhiko Ohashi
  Parasite (Paris, France), 28, 9, 9, 2021, [International Magazine]
  English, Scientific journal, Poultry red mites (PRMs, Dermanyssus gallinae) are ectoparasites that negatively affect farmed chickens, leading to serious economic losses worldwide. Acaricides have been used to control PRMs in poultry houses. However, some PRMs have developed resistance to acaricides, and therefore different approaches are required to manage the problems caused by PRMs. Vaccination of chickens is one of the methods being considered to reduce the number of PRMs in poultry houses. In a previous study, a cysteine protease, Deg-CPR-1, was identified as a candidate vaccine against PRMs distributed in Europe. In this study, we investigated the characteristics of Deg-CPR-1. A phylogenetic analysis revealed that Deg-CPR-1 is closely related to the digestive cysteine proteases of other mite species, and it was classified into a cluster different from that of chicken cathepsins. Deg-CPR-1 of PRMs in Japan has an amino acid substitution compared with that of PRMs in Europe, but it showed efficacy as a vaccine, consistent with previous findings. Deg-CPR-1 exhibited cathepsin L-like enzyme activity. In addition, the Deg-CPR-1 mRNA was expressed in the midgut and in all stages of PRMs that feed on blood. These results imply that Deg-CPR-1 in the midgut may have important functions in physiological processes, and the inhibition of its expression may contribute to the efficacy of a Deg-CPR-1-based vaccine. Further research is required to fully understand the mechanisms of vaccine efficacy.
• Anatomy and histology of the foramen of ovarian bursa opening to the peritoneal cavity and its changes in autoimmune disease-prone mice.
  Marina Hosotani; Osamu Ichii; Teppei Nakamura; Takashi Namba; Md Rashedul Islam; Yaser Hosny Ali Elewa; Takafumi Watanabe; Hiromi Ueda; Yasuhiro Kon
  Journal of anatomy, 238, 1, 73, 85, Jan. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The ovarian bursa is a small peritoneal cavity enclosed by the mesovarium and mesosalpinx, which surrounds the ovaries and oviductal infundibulum in mammals. The ovarian bursa is considered as the structure facilitating the transport of ovulated oocytes into the oviduct. Our previous study revealed reduced oocyte pick-up function in the oviduct of lupus-prone MRL/MpJ-Faslpr/lpr mouse, suggesting the possibility of an escape of ovulated oocytes into the peritoneal cavity, despite the presence of an almost complete ovarian bursa in the mouse. In this study, we revealed anatomical and histological characteristics of the ovarian bursa in C57BL/6 N, MRL/MpJ, and MRL/MpJ-Faslpr/lpr mice. All strains had the foramen of ovarian bursa (FOB), with a size of approximately 0.04 to 0.12 cm2 , surrounded by the ligament of ovarian bursa (LOB), which is part of the mesosalpinx. The LOB was partially lined with the cuboidal mesothelial cells and consisted of a thick smooth muscle layer in all strains. In 6-month-old MRL/MpJ-Faslpr/lpr mice, in which the systemic autoimmune abnormality deteriorated and oocyte pick-up function was impaired, the size of the FOB tended to be larger than that of other strains. Additionally, in MRL/MpJ-Faslpr/lpr mice at 6 months of age, there was infiltration by numerous immune cells in the mesosalpinx suspending the isthmus; however, the LOB prevented severe inflammation and showed deposition of collagen fibers. These results not only indicate that the FOB is a common structure within mice, but also imply the physiological function of the LOB and its role in maintaining the microenvironment around the ovary, as well as regulating healthy reproduction.
• Distal Phalanx Osteolysis and Subcutaneous Calcinosis in CREST Syndrome.
  Taro Horino; Osamu Ichii
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 27, 1, e22, 01 Jan. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Group A Streptococcus Infection-Induced Polyarteritis Nodosa in an Adult Patient Complicated With Diffuse Fasciitis.
  Taro Horino; Osamu Ichii; Tatsuki Matsumoto; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 26, 8, S579-S580, Dec. 2020, [International Magazine]
  English, Scientific journal
• Unique Running Pattern and Mucosal Morphology Found in the Colon of Cotton Rats
  Chuluunbaatar, T.; Ichii, O.; Nakamura, T.; Irie, T.; Namba, T.; Islam, M.R.; Otani, Y.; Masum, M.A.; Okamatsu-Ogura, Y.; Elewa, Y.H.A.; Kon, Y.
  Frontiers in Physiology, 11, Frontiers Media {SA}, Oct. 2020, [Peer-reviewed]
  Scientific journal
• Suppurative Osteomyelitis Incorrectly Diagnosed as Rheumatoid Arthritis.
  Taro Horino; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 26, 7, e261-e263, Oct. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Antineutrophil Cytoplasmic Antibody-Associated Hypertrophic Pachymeningitis Mimicking Petroclival Meningioma.
  Taro Horino; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 26, 6, e153-e154, Sep. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Long-term Imaging Findings on Serial FDG PET/CT Scans in a Patient With Polymyositis.
  Taro Horino; Tatsuki Matsumoto; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 26, 6, e174-e175, Sep. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Hydroxychloroquine-Associated Hyperpigmentation in Chilblain Lupus Erythematosus.
  Taro Horino; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 26, 6, e192, Sep. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Anomalous Inferior Vena Cava in a Patient With Limited Cutaneous Systemic Sclerosis and Primary Biliary Cholangitis.
  Taro Horino; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 26, 6, e203, Sep. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Takayasu Arteritis Presenting as Renovascular Hypertension.
  Taro Horino; Osamu Ichii; Tatsuki Matsumoto; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 26, 5, e94, Aug. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Altered hepatic cytochrome P450 expression in cats after chronic exposure to decabromodiphenyl ether (BDE-209).
  Kraisiri Khidkhan; Hazuki Mizukawa; Yoshinori Ikenaka; Shouta M M Nakayama; Kei Nomiyama; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Shinsuke Tanabe; Mayumi Ishizuka
  The Journal of veterinary medical science, 82, 7, 978, 982, 31 Jul. 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The knowledge of cytochrome P450 (CYP) expression involved in chemical exposure are necessary in clinical applications for the medication and prediction of adverse effects. The aim of this study was to evaluate the mRNA expression of CYP1-CYP3 families in cats exposed to BDE-209 for one year. All selected CYP isoforms showed no significant difference in mRNA expressions between control and exposure groups, however, CYP3A12 and CYP3A131 revealed tend to be two times higher in the exposure group compared to control group. The present results indicate that the chronic exposure of BDE209 could not alter CYP expression in the liver of cats. This result considered caused by the deficiency of CYP2B subfamily which is major metabolism enzyme of polybrominated diphenyl ethers (PBDEs) in cat.
• Comparative Analyses of the Antiviral Activities of IgG and IgA Antibodies to Influenza A Virus M2 Protein.
  Kosuke Okuya; Nao Eguchi; Rashid Manzoor; Reiko Yoshida; Shinji Saito; Tadaki Suzuki; Michihito Sasaki; Takeshi Saito; Yurie Kida; Akina Mori-Kajihara; Hiroko Miyamoto; Osamu Ichii; Masahiro Kajihara; Hideaki Higashi; Ayato Takada
  Viruses, 12, 7, 20 Jul. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The influenza A virus (IAV) matrix-2 (M2) protein is an antigenically conserved viral envelope protein that plays an important role in virus budding together with another envelope protein, hemagglutinin (HA). An M2-specific mouse monoclonal IgG antibody, rM2ss23, which binds to the ectodomain of the M2 protein, has been shown to be a non-neutralizing antibody, but inhibits plaque formation of IAV strains. In this study, we generated chimeric rM2ss23 (ch-rM2ss23) IgG and IgA antibodies with the same variable region and compared their antiviral activities. Using gel chromatography, ch-rM2ss23 IgA were divided into three antibody subsets: monomeric IgA (m-IgA), dimeric IgA (d-IgA), and trimeric and tetrameric IgA (t/q-IgA). We found that t/q-IgA had a significantly higher capacity to reduce the plaque size of IAVs than IgG and m-IgA, most likely due to the decreased number of progeny virus particles produced from infected cells. Interestingly, HA-M2 colocalization was remarkably reduced on the infected cell surface in the presence of ch-rM2ss23 antibodies. These results indicate that anti-M2 polymeric IgA restricts IAV budding more efficiently than IgG and suggest a role of anti-M2 IgA in cross-protective immunity to IAVs.
• Antineutrophil cytoplasmic antibody-associated vasculitis in diffuse large B cell lymphoma.
  Taro Horino; Osamu Ichii
  Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia, 41, 2, 215, 216, 18 Jul. 2020, [Peer-reviewed], [International Magazine]
  English
• Hepatitis C Virus-Associated Cryoglobulinemic Vasculitis.
  Taro Horino; Osamu Ichii; Satoshi Inotani; Tatsuki Matsumoto; Yoshio Terada
  The American journal of medicine, 133, 7, e367-e368, Jul. 2020, [Peer-reviewed], [International Magazine]
  English
• Activities of matrix metalloproteinase-2, matrix metalloproteinase-9, and serine proteases in samples of the colorectal mucosa of Miniature Dachshunds with inflammatory colorectal polyps.
  Noriyuki Nagata; Hiroshi Ohta; Arisa Yamada; Yong Bin Teoh; Osamu Ichii; Keitaro Morishita; Noboru Sasaki; Mitsuyoshi Takiguchi
  American journal of veterinary research, 81, 7, 572, 580, Jul. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To investigate the activities of gelatinases (matrix metalloproteinase [MMP]-2 and MMP-9) and serine proteases in the colorectal mucosa of Miniature Dachshunds (MDs) with inflammatory colorectal polyps (ICRPs). ANIMALS: 15 MDs with ICRPs and 5 dogs with non-ICRP-related large bowel diarrhea (controls). PROCEDURES: Zymographic methods were used to evaluate the activities of MMP-2, MMP-9, latent forms of MMP-2 and MMP-9 (pro-MMP-2 and pro-MMP-9), and serine proteases in inflamed and noninflamed tissue samples from MDs with ICRPs and in noninflamed tissue samples from control dogs. The associations of serine protease activities with MMP-2 or MMP-9 activity were also analyzed. RESULTS: Activities of pro-MMP-2 and pro-MMP-9 were detected in most tissue samples, regardless of the tissue type, whereas activities of MMP-2 and MMP-9 were not detected in control tissue samples. In the inflamed tissue samples from MDs with ICRPs, the activities of MMP-2, pro-MMP-9, and MMP-9 were significantly higher than those in the noninflamed tissue samples from those dogs. Serine protease activities were significantly higher in the inflamed and noninflamed tissue samples from MDs with ICRP, compared with findings for control tissue samples. A weak correlation was detected between serine protease activities and MMP-9 activity. CONCLUSIONS AND CLINICAL RELEVANCE: Study results suggested that gelatinase and serine protease activities are upregulated in the colorectal mucosa of MDs with ICRPs, possibly contributing to the pathogenesis of this disease through the functions of these enzymes in degradation of extracellular matrix and promotion of inflammatory cell migration and inflammatory responses.
• BXSB/MpJ-Yaa mouse model of systemic autoimmune disease shows increased apoptotic germ cells in stage XII of the seminiferous epithelial cycle.
  Yuki Otani; Osamu Ichii; Md Abdul Masum; Junpei Kimura; Teppei Nakamura; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Cell and tissue research, 381, 1, 203, 216, Springer Science and Business Media {LLC}, Jul. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, In mammals, the reproductive system and autoimmunity regulate mutual functions. Importantly, systemic autoimmune diseases are thought to cause male infertility but the underlying pathological mechanism remains unclear. In this study, the morpho-function of the testes in BXSB/MpJ-Yaa mice was analyzed as a representative mouse model for systemic autoimmune diseases to investigate the effect of excessive autoimmunity on spermatogenesis. At 12 and 24 weeks of age, BXSB/MpJ-Yaa mice showed splenomegaly and increased levels of serum autoantibodies, whereas no controls showed a similar autoimmune condition. In histological analysis, the enlarged lumen of the seminiferous tubules accompanied with scarce spermatozoa in the epididymal ducts were observed in some of the BXSB/MpJ-Yaa and BXSB/MpJ mice but not in C57BL/6N mice. Histoplanimetrical analysis revealed significantly increased residual bodies and apoptotic germ cells in the seminiferous tubules in BXSB/MpJ-Yaa testes without apparent inflammation. Notably, in stage XII of the seminiferous epithelial cycles, the apoptotic germ cell number was remarkably increased, showing a significant correlation with the indices of systemic autoimmune disease in BXSB/MpJ-Yaa mice. Furthermore, the Sertoli cell number was reduced at the early disease stage, which likely caused subsequent morphological changes in BXSB/MpJ-Yaa testes. Thus, our histological study revealed the altered morphologies of BXSB/MpJ-Yaa testes, which were not observed in controls and statistical analysis suggested the effects of an autoimmune condition on this phenotype, particularly the apoptosis of meiotic germ cells. BXSB/MpJ-Yaa mice were shown to be an efficient model to study the relationship between systemic autoimmune disease and the local reproductive system.
• Histopathological changes in tear-secreting tissues and cornea in a mouse model of autoimmune disease.
  Masaya Hiraishi; Md Abdul Masum; Takashi Namba; Yuki Otani; Yaser Ha Elewa; Osamu Ichii; Yasuhiro Kon
  Experimental biology and medicine (Maywood, N.J.), 245, 12, 999, 1008, Jun. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, IMPACT STATEMENT: Cornea, an outermost layer of mammalian eye, is protected by tear film and abnormalities of tear film causes dry eye. Dry eye injures the cornea which results lower vision in patients. Several factors cause dry eye, including altered systemic conditions, environment, and immunological abnormality of the patient in autoimmune disease like Sjögren's syndrome (SS). However, the detailed pathology of autoimmune abnormality-mediated dry eye is unclear. Here we demonstrated that systemic autoimmune abnormality in BXSB-Yaa mice was associated with histological changes in the exocrine glands and cornea of the eyes. We also showed that BXSB-Yaa mice developed mild or early stage dry eye-like disease and explain the existence of a compensatory mechanism associated with the dysfunction of these tissues. Thus, BXSB-Yaa could be a model for SS-like disease-associated dry eye and these data would contribute to the understanding of the pathogenesis of autoimmune-related dry eye disease.
• Potential Role of Nonneutralizing IgA Antibodies in Cross-Protective Immunity against Influenza A Viruses of Multiple Hemagglutinin Subtypes.
  Kosuke Okuya; Reiko Yoshida; Rashid Manzoor; Shinji Saito; Tadaki Suzuki; Michihito Sasaki; Takeshi Saito; Yurie Kida; Akina Mori-Kajihara; Tatsunari Kondoh; Masahiro Sato; Masahiro Kajihara; Hiroko Miyamoto; Osamu Ichii; Hideaki Higashi; Ayato Takada
  Journal of virology, 94, 12, 01 Jun. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, IgA antibodies on mucosal surfaces are known to play an important role in protection from influenza A virus (IAV) infection and are believed to be more potent than IgG for cross-protective immunity against IAVs of multiple hemagglutinin (HA) subtypes. However, in general, neutralizing antibodies specific to HA are principally HA subtype specific. Here, we focus on nonneutralizing but broadly cross-reactive HA-specific IgA antibodies. Recombinant IgG, monomeric IgA (mIgA), and polymeric secretory IgA (pSIgA) antibodies were generated based on the sequence of a mouse anti-HA monoclonal antibody (MAb) 5A5 that had no neutralizing activity but showed broad binding capacity to multiple HA subtypes. While confirming that there was no neutralizing activity of the recombinant MAbs against IAV strains A/Puerto Rico/8/1934 (H1N1), A/Adachi/2/1957 (H2N2), A/Hong Kong/483/1997 (H5N1), A/shearwater/South Australia/1/1972 (H6N5), A/duck/England/1/1956 (H11N6), and A/duck/Alberta/60/1976 (H12N5), we found that pSIgA, but not mIgA and IgG, significantly reduced budding and release of most of the viruses from infected cells. Electron microscopy demonstrated that pSIgA deposited newly produced virus particles on the surfaces of infected cells, most likely due to tethering of virus particles. Furthermore, we found that pSIgA showed significantly higher activity to reduce plaque sizes of the viruses than IgG and mIgA. These results suggest that nonneutralizing pSIgA reactive to multiple HA subtypes may play a role in intersubtype cross-protective immunity against IAVs.IMPORTANCE Mucosal immunity represented by pSIgA plays important roles in protection from IAV infection. Furthermore, IAV HA-specific pSIgA antibodies are thought to contribute to cross-protective immunity against multiple IAV subtypes. However, the mechanisms by which pSIgA exerts such versatile antiviral activity are not fully understood. In this study, we generated broadly cross-reactive recombinant IgG and pSIgA having the same antigen-recognition site and compared their antiviral activities in vitro These recombinant antibodies did not show "classical" neutralizing activity, whereas pSIgA, but not IgG, significantly inhibited the production of progeny virus particles from infected cells. Plaque formation was also significantly reduced by pSIgA, but not IgG. These effects were seen in infection with IAVs of several different HA subtypes. Based on our findings, we propose an antibody-mediated host defense mechanism by which mucosal immunity may contribute to broad cross-protection from IAVs of multiple HA subtypes, including viruses with pandemic potential.
• Altered ciliary morphofunction in the oviductal infundibulum of systemic autoimmune disease-prone MRL/MpJ-Faslpr/lpr mice.
  Marina Hosotani; Osamu Ichii; Teppei Nakamura; Md Abdul Masum; Yuki Otani; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Cell and tissue research, 380, 3, 627, 641, Jun. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, According to our previous reports, impaired oocyte pickup was observed in the oviductal infundibulum of an autoimmune disease (AD) mouse model, suggesting a relationship between female infertility and AD. This study examines the relationship between AD and infundibulum morphofunction by focusing on the epithelial cilia. Healthy MRL/MpJ and AD-prone MRL/MpJ-Faslpr/lpr mice were examined at 3 and 6 months of age, representing early and late disease stages, respectively. Oocyte pickup indices decreased with AD progression indicated by splenomegaly, autoantibody production and increased T cell counts of infundibulum mucosa in MRL/MpJ-Faslpr/lpr mice. Ciliary beating frequency (CBF) and height in the infundibulum were faster and higher in MRL/MpJ-Faslpr/lpr mice than in MRL/MpJ mice at the early AD stages, although the absolute CBF values were lower at the late AD stage. At the late stage, ciliary height did not differ between mouse lines but the morphological index of cilia beating direction indicated randomized patterns in MRL/MpJ-Faslpr/lpr mice. The tracheal mucosa was also examined as a representative example of cilia morphology; its CBF decreased at the late AD stage in MRL/MpJ-Faslpr/lpr; however, there were no AD-related morphological changes. Our results demonstrate altered cilia motility in systemic and reproductive organs, with such morphological changes of the infundibulum likely impairing function, including oocyte pickup.
• Anti-Ku antibody-related scleroderma-polymyositis overlap syndrome associated with thrombocytopaenia.
  T Horino; H Nishikawa; S Inotani; T Matsumoto; O Ichii; Y Terada
  QJM : monthly journal of the Association of Physicians, 113, 5, 349, 350, 01 May 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Expression of Peroxisome Proliferator-activated Receptor-γ in the Kidneys of Cats with Chronic Kidney Disease.
  A Yabuki; Y Uehara; O Ichii; C Yoshida; O Yamato
  Journal of comparative pathology, 176, 81, 85, Apr. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Peroxisome proliferator-activated receptor (PPAR)-γ plays an important role in various cellular functions and its activation exerts protective effects in kidney diseases. In the present study, chronic kidney disease in cats was examined, and changes in renal expression of PPARγ were observed by use of immunohistochemistry. In normal kidneys, nuclei of the superficial cortical tubules, medullary tubules and glomerular cells expressed PPARγ. The vascular walls (tunica media) also showed positive expression. In diseased kidneys, the expression of PPARγ varied between the cases. Some cases showed strong expression, while others had weak expression. PPARγ expression in the nuclei of infiltrating mononuclear cells was also detected in over half of the cases. Although there was no significant relationship between the expression of renal PPARγ and the severity of kidney disease, the fact that there were many cases where the expression of renal PPARγ was reduced was an important finding, and might be one of the possible mechanisms underlying feline chronic kidney diseases.
• Morphological and molecular identification of cyathostomine gastrointestinal nematodes of Murshidia and Quilonia species from Asian elephants in Myanmar.
  Hla Myet Chel; Takashi Iwaki; Myint Myint Hmoon; Yu Nandi Thaw; Nyein Chan Soe; Shwe Yee Win; Saw Bawm; Lat Lat Htun; Mar Mar Win; Zaw Min Oo; Md Abdul Masum; Osamu Ichii; Ryo Nakao; Nariaki Nonaka; Ken Katakura
  International journal for parasitology. Parasites and wildlife, 11, 294, 301, Apr. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Gastrointestinal nematode parasites have long been recognized in Asian elephants. The most common parasites belong to the subfamily Cyathostominae of the family Strongylidae, which are small to medium-sized with a cylindrical buccal capsule surrounded by coronal leaflets. Diagnostic keys of such parasites are provided from old illustrations in the form of line drawings. However, there very few photomicrographs and no genetic information of these parasites exist. In the present study we obtained adult worm specimens from faeces of Asian elephants after anthelmintic treatment in two elephant camps in Myanmar. Here, we provided photomicrographs for five cyathostomine parasites, Murshidia falcifera, Murshidia indica, Murshidia neveulemairei, Quilonia renniei, and Quilonia travancra almost 100 years after their original drawings. In addition, we determined the mitochondrial cytochrome c oxidase subunit I (COI) gene sequences of these species. Phylogenetic analysis of the COI genes of Murshidia and Quilonia species from Asian and African elephants revealed parasite speciation in each elephant host. The present study also indicated that several Murshidia and Quilonia species were widely distributed in Asian elephants in Myanmar, providing new insight into control strategies and evolution of cyathostomine gastrointestinal parasites in elephants.
• Macromolecular creatine kinase Type 1 in a patient with anti-aminoacyl-tRNA synthetase autoantibody-related dermatomyositis.
  T Horino; S Inotani; T Matsumoto; O Ichii
  QJM : monthly journal of the Association of Physicians, 113, 3, 201, 202, 01 Mar. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Autoimmune polyglandular syndrome type 3 variant in rheumatoid arthritis.
  Taro Horino; Masami Ogasawara; Osamu Ichii; Yoshio Terada
  Romanian journal of internal medicine = Revue roumaine de medecine interne, 58, 1, 40, 43, 01 Mar. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, INTRODUCTION: Although type 1 diabetes mellitus is largely associated with autoimmune thyroid disease and this entity has been recently referred to as autoimmune polyglandular syndrome type 3 variant, the autoimmune polyglandular syndrome type 3 variant in patients with rheumatoid arthritis has not been reported so far. We herein describe the first case of rheumatoid arthritis that was associated with autoimmune polyglandular syndrome type 3 variant. CASE REPORT: A 77-year-old woman with a 15-year history of rheumatoid arthritis (RA) and a 10-year history of type 2 diabetes mellitus (T2D) presented with polyarthralgia and hyperglycaemia. Methotrexate 16 mg/week had been started from the onset and was continued, and adalimumab 40 mg/day was started for RA. Insulin treatment was also started for the diabetes. Laboratory examinations revealed high levels of C-reactive protein (CRP), rheumatoid factor, anti-cyclic citrullinated peptide antibody, and matrix metalloprotease 3. She was admitted multiple times as the symptoms recurred after treatment. Subsequently, based on the clinical course and investigations, she was diagnosed with type 1 diabetes mellitus and Graves' disease occurring during the course of RA and T2D. Her clinical course improved after reinforcement of insulin therapy and the addition of thiamazole therapy. CONCLUSION: In patients with rheumatoid arthritis, the autoimmune polyglandular syndrome type 3 variant should be considered as the cause of the deterioration.
• Immune-associated renal disease found in caspase 3-deficient mice.
  Takashi Suzuki; Osamu Ichii; Teppei Nakamura; Taro Horino; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Cell and tissue research, 379, 2, 323, 335, Springer Science and Business Media {LLC}, Feb. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Caspase (CASP) 3 is known as a representative effector CASP of apoptosis and recently as a mediator in inflammatory cell death called pyroptosis. Interestingly, homozygotes of Casp3 knockout (KO) mice with 129-background show complete embryonic lethality; however, some of those with C57BL/6 (B6)-background (B6.129S1-Casp3tm1Flv/J) survived at a lower rate (KO, 11%; WT, 22%), developing immune abnormality-associated renal phenotypes. Homozygotes of Casp3 KO mice with B6-background that survived for 8-12 months showed abnormality in the kidney and spleen but not in other organs. Briefly, these Casp3 KO kidneys showed proliferative glomerular lesions characterized by increased cells, matrices, immune complex depositions containing IgA and complement 3 in the mesangial area, podocyte injuries and inflammatory cell infiltrations in the tubulointerstitium. However, severe membranous lesion or renal dysfunction was not observed. Increased expression of inflammation-associated gene sets and inflammatory Casps, including Casp12, was observed in these Casp3 KO kidneys. Moreover, these Casp3 KO mice showed mild splenomegaly compared with WT mice. Thus, the long-surviving Casp3 KO mice with B6-background developed renal lesions with altered immune conditions. CASP3 deficiency and aging factors could affect this phenotype by altering the function and/or development of each cell in the kidney and immune organs.
• Unique morphological characteristics in the ovary of cotton rat (Sigmodon hispidus)
  Md. Rashedul ISLAM; Osamu ICHII; Teppei NAKAMURA; Takao IRIE; Md. Abdul MASUM; Marina HOSOTANI; Yuki OTANI; Yaser Hosny Ali ELEWA; Yasuhiro KON
  Journal of Reproduction and Development, 66, 6, 529, 538, Japanese Society of Animal Reproduction, 2020, [Peer-reviewed], [Domestic magazines]
  English, Cotton rats (Sigmodon hispidus, CRs) are commonly used as animal models in biomedical research. However, the reproductive characteristics and ovarian development in the CRs has not been widely investigated. We have previously shown that female CRs, in particular, show several unique phenotypes associated with the urogenital system, such as chronic kidney disease and pyometra. Our investigation revealed unique morphologies in CR ovaries, particularly in oocytes. Cotton rat ovaries at 6-8 weeks of age were obtained from the Hokkaido Institute of Public Health, and their sections analyzed by light microscopy and transmission electron microscopy. Although the general histology and folliculogenesis of CR ovaries were similar to those of other experimental rodents, multi-oocyte follicles (MOFs) and double nucleated oocytes (DNOs) were also observed. Although MOFs were found at all stages of follicular development, a greater frequency of MOFs was observed in the primary and secondary stages. However, DNOs tended to be frequently observed in primordial follicles. Almost all MOF oocytes and a few DNOs possessed a clear zona pellucida, expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and Forkhead box protein 2, a representative marker of oocytes and follicular epithelial cells. Thus, our investigations revealed the unique phenotypes of the CR ovary. As MOFs and DNOs are occasionally observed in human patients with infertility, the CR would be a useful animal model to study for gaining a better understanding of folliculogenesis and oocytogenesis, as well as their abnormalities in humans and other animals.
• PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade.
  Otgontuya Ganbaatar; Satoru Konnai; Tomohiro Okagawa; Yutaro Nojima; Naoya Maekawa; Erina Minato; Atsushi Kobayashi; Ryo Ando; Nobuya Sasaki; Daisuke Miyakoshi; Osamu Ichii; Yukinari Kato; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi
  PloS one, 15, 11, e0234218, 2020, [International Magazine]
  English, Scientific journal, Programmed death-1 (PD-1) is an immunoinhibitory receptor expressed on lymphocytes. Interaction of PD-1 with its ligand PD-ligand 1 (PD-L1) delivers inhibitory signals and impairs proliferation, cytokine production, and cytotoxicity of T cells. In our previous studies, we have developed anti-bovine PD-L1 monoclonal antibodies (mAbs) and reported that the PD-1/PD-L1 pathway was closely associated with T-cell exhaustion and disease progression in bovine chronic infections and canine tumors. Furthermore, we found that blocking antibodies that target PD-1 and PD-L1 restore T-cell functions and could be used in immunotherapy in cattle and dogs. However, the immunological role of the PD-1/PD-L1 pathway for chronic equine diseases, including tumors, remains unclear. In this study, we identified cDNA sequences of equine PD-1 (EqPD-1) and PD-L1 (EqPD-L1) and investigated the role of anti-bovine PD-L1 mAbs against EqPD-L1 using in vitro assays. In addition, we evaluated the expression of PD-L1 in tumor tissues of equine malignant melanoma (EMM). The amino acid sequences of EqPD-1 and EqPD-L1 share a considerable identity and similarity with homologs from non-primate species. Two clones of the anti-bovine PD-L1 mAbs recognized EqPD-L1 in flow cytometry, and one of these cross-reactive mAbs blocked the binding of equine PD-1/PD-L1. Of note, immunohistochemistry confirmed the PD-L1 expression in EMM tumor tissues. A cultivation assay revealed that PD-L1 blockade enhanced the production of Th1 cytokines in equine immune cells. These findings showed that our anti-PD-L1 mAbs would be useful for analyzing the equine PD-1/PD-L1 pathway. Further research is warranted to discover the immunological role of PD-1/PD-L1 in chronic equine diseases and elucidate a future application in immunotherapy for horses.
• Vasculature-Associated Lymphoid Tissue: A Unique Tertiary Lymphoid Tissue Correlates With Renal Lesions in Lupus Nephritis Mouse Model.
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Yuki Otani; Takashi Namba; Yasuhiro Kon
  Frontiers in immunology, 11, 595672, 595672, 2020, [International Magazine]
  English, Scientific journal, Lupus nephritis (LN) is a common complication in young patients and the most predominant cause of glomerulonephritis. Infiltrating immune cells and presence of immunocomplexes in the kidney are hallmarks of LN, which is closely associated with renal lesions (RLs). However, their regulatory mechanism in the kidney remains unclear, which is valuable for prevention of RL development. Here, we show the development of vasculature-associated lymphoid tissue (VALT) in LN, which is related to renal inflammatory cytokines, indicating that VALT is a unique tertiary lymphoid tissue. Transcriptomic analysis revealed different chemokines and costimulatory molecules for VALT induction and organization. Vascular and perivascular structures showed lymphoid tissue organization through lymphorganogenic chemokine production. Transcriptional profile and intracellular interaction also demonstrated antigen presentation, lymphocyte activity, clonal expansion, follicular, and germinal center activity in VALT. Importantly, VALT size was correlated with infiltrating immune cells in kidney and RLs, indicating its direct correlation with the development of RLs. In addition, dexamethasone administration reduced VALT size. Therefore, inhibition of VALT formation would be a novel therapeutic strategy against LN.
• Podocyte Injury Through Interaction Between Tlr8 and Its Endogenous Ligand miR-21 in Obstructed and Its Collateral Kidney.
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in immunology, 11, 606488, 606488, 2020, [International Magazine]
  English, Scientific journal, While chronic kidney disease is prevalent in adults, obstructive nephropathy (ON) has been reported in both young and old patients. In ON, tubulointerstitial lesions (TILs) have been widely investigated, but glomerular lesions (GLs) have been largely neglected. Here, we show a novel mechanism underlying GL development in ON in young and old mice. TILs develop earlier than GLs owing to infiltration of inflammatory cells in the tubulointerstitium, but GLs develop following the activation of Toll-like receptor 8 (Tlr8) even though the absence of inflammatory cells infiltrating the glomerulus. TLR8 and interleukin 1 beta (IL1β) proteins colocalize with reducing podocyte function markers (PFMs), indicating the activation of TLR8 signaling in injured podocytes. Furthermore, glomerular and serum levels of miR-21, an endogenous ligand for Tlr8, were higher in the ON mouse model than in the sham control. The glomerular expression of Tlr8 positively correlates with miR-21 and the downstream cytokines Il1b and Il6 and negatively correlated with PFMs (Nphs1 and Synpo). We also show the colocalization of TLR8 and IL1β proteins with reducing PFMs in both obstructed and collateral kidney of young and old mice. Furthermore, in vitro study results revealed higher expression of Tlr8 and its downstream cytokines in glomeruli from obstructed kidneys following treatment with miR-21 mimic than in the control. In conclusion, the overexpression of Tlr8 may serve as a plausible mechanism underlying GL development in ON through podocyte injury.
• Age-related glomerular lesions with albuminuria in male cotton rats.
  Osamu Ichii; Teppei Nakamura; Takao Irie; Yuki Otani; Marina Hosotani; Md Abdul Masum; Rashedul Md Islam; Taro Horino; Yuji Sunden; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Histochemistry and cell biology, 153, 1, 27, 36, Jan. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The increased prevalence of aging-related chronic kidney disease (CKD) among humans is a problem worldwide. Aged cotton rats (Sigmodon hispidus) are considered novel model animals for studying CKD, especially as the females develop severe tubulointerstitial lesions with anemia. To investigate the renal pathologic features in aged male cotton rats and their characteristic glomerular injuries, the animals were divided into young, adult, old-aged, and advanced-aged groups (1-4, 5-8, 9-12, and 13-17 months, respectively) and pathologically analyzed. Anemia and renal dysfunction, as indicated by hematologic and serologic parameters, were significantly milder in the advanced-aged males than in the old-aged females. The males had increased urinary albumin-to-creatinine ratios from the old-age period, with the advanced-aged males having significantly higher levels than those in the old-aged females and young males. The old-aged females did not show clear glomerular injuries, whereas the advanced-aged males showed membranous lesions characterized by irregular and thickened glomerular basement membranes (GBMs). Characteristically, several large-sized projections from the GBM toward the podocytes were observed by microscopy, and podocytes covering these projections effaced their foot processes. The advanced-aged males showed aging-related IgG immune-complex depositions in the paramesangial regions and along the GBM. Furthermore, the positive reaction for podocin (a podocyte molecule) was granulated along the GBM. Thus, we clarified the albuminuria associated with altered glomerular structures in advanced-aged cotton rats, and that these phenotypes were closely associated with aging. These data help to clarify the aging-related pathogenesis of glomerular injury.
• Intestinal Spirochetosis Caused by Brachyspira pilosicoli in a Systemic Lupus Erythematosus Patient.
  Tomohiro Eguchi; Kosuke Inoue; Taro Horino; Tatsuki Matsumoto; Saya Kamioka; Yoshie Nishida; Miyuki Morimoto; Norihito Morimoto; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 25, 8, e142-e145, Dec. 2019, [Peer-reviewed], [International Magazine]
  English
• Molecular characterization of three Sarcocystis spp. from wild sika deer (Cervus nippon yesoensis) in Hokkaido, Japan.
  Takao Irie; Osamu Ichii; Teppei Nakamura; Tetsuya Ikeda; Takuya Ito; Akiko Yamazaki; Shinji Takai; Kinpei Yagi
  Veterinary parasitology, regional studies and reports, 18, 100327, 100327, Dec. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Tissue distribution and characterization of feline cytochrome P450 genes related to polychlorinated biphenyl exposure.
  Kraisiri Khidkhan; Hazuki Mizukawa; Yoshinori Ikenaka; Shouta M M Nakayama; Kei Nomiyama; Nozomu Yokoyama; Osamu Ichii; Wageh Sobhy Darwish; Mitsuyoshi Takiguchi; Shinsuke Tanabe; Mayumi Ishizuka
  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 226, 108613, 108613, Dec. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Cats have been known to be extremely sensitive to chemical exposures. To understand these model species' sensitivity to chemicals and their toxicities, the expression profiles of xenobiotic-metabolizing enzymes should be studied. Unfortunately, the characterization of cytochrome P450 (CYP), the dominant enzyme in phase I metabolism, in cats has not extensively been studied. Polychlorinated biphenyls (PCBs) are known as CYP inducers in animals, but the information regarding the PCB-induced CYP expression in cats is limited. Therefore, in the present study, we aimed to elucidate the mRNA expression of the CYP1-CYP3 families in the cat tissues and to investigate the CYP mRNA expression related to PCB exposure. In cats, the greatest abundance of CYP1-CYP3 (CYP1A2, CYP2A13, CYP2C41, CYP2D6, CYP2E1, CYP2E2, CYP2F2, CYP2F5, CYP2J2, CYP2U1, and CYP3A132) was expressed in the liver, but some extrahepatic isozymes were found in the kidney (CYP1A1), heart (CYP1B1), lung (CYP2B11 and CYP2S1) and small intestine (CYP3A131). In cats, CYP1A1, CYP1A2 and CYP1B1 were significantly upregulated in the liver as well as in several tissues exposed to PCBs, indicating that these CYPs were distinctly induced by PCBs. The strong correlations between 3,3',4,4'-tetrachlorobiphenyl (CB77) and CYP1A1 and CYP1B1 mRNA expressions were noted, demonstrating that CB77 could be a potent CYP1 inducer. In addition, these CYP isoforms could play an essential role in the PCBs biotransformation, particularly 3-4 Cl-PCBs, because a high hydroxylated metabolite level of 3-4 Cl-OH-PCBs was observed in the liver.
• Slc:Hartley guinea pigs frequently possess duplication of the caudal vena cava.
  Teppei Nakamura; Miyuki Norimura; Kanako Sumi; Osamu Ichii; Yaser Hosny Ali Elewa; Yasuhiro Kon; Osamu Tatsumi; Hideki Hattori; Tomoji Yoshiyasu; Ken-Ichi Nagasaki
  Experimental animals, 68, 4, 465, 470, Japanese Association for Laboratory Animal Science, 06 Nov. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The formation of the caudal vena cava is a complex process involving development, regression, and anastomosis. In mammals, the normal caudal vena cava runs to the right side of the abdominal aorta, while duplication of the caudal vena cava has been identified as a congenital abnormality in both companion animals and humans. The present study demonstrates that Slc:Hartley guinea pigs frequently possess asymptomatic duplicated caudal vena cava. The prevalence was 30% and 24% for males and females, respectively, with no sex-related differences. In accordance with Saad et al. (2012)'s criteria, duplicated caudal vena cava were classified into two distinct variations. The dominant variation was a complete duplication without iliac anastomosis where the left caudal vena cava continued from the left common iliac vein and joined the left renal vein; the left renal vein ran to the right to join the right caudal vena cava. The alternative variation was an incomplete duplication where the left caudal vena cava joined the right infrarenal caudal vena cava at a more cranial point than in normal cases; the renal segment was unchanged. Iliac anastomosis was not found in any cases. Duplicated caudal vena cava neither affected the body weight nor the kidney weight. In conclusion, Slc:Hartley guinea pigs frequently possess asymptomatic duplicated caudal vena cava in the absence of iliac anastomosis and appear to be a novel and useful animal model for duplicated caudal vena cava in animals and humans.
• Age-related changes in bone morphology, function, and cell populations in inbred C57BL/6N mice
  Takashi Namba; Osamu Ichii; Teppei Nakamura; Md. Abdul Masum; Yuki Otani; Saori Otsuka-Kanazawa; Yaser Elewa; Yasuhiro Kon
  Japanese Journal of Veterinary Research, 67, 4, 313, 317, Nov. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Reactive Arthritis Caused by Rothia mucilaginosa in an Elderly Diabetic Patient.
  Taro Horino; Satoshi Inotani; Tatsuki Matsumoto; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 26, 8, e303-e304, 23 Oct. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Effects of PCB exposure on serum thyroid hormone levels in dogs and cats.
  Kohki Takaguchi; Hiroyuki Nishikawa; Hazuki Mizukawa; Rumi Tanoue; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Shouta M M Nakayama; Yoshinori Ikenaka; Tatsuya Kunisue; Mayumi Ishizuka; Shinsuke Tanabe; Hisato Iwata; Kei Nomiyama
  The Science of the total environment, 688, 1172, 1183, 20 Oct. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH-PCBs) might disrupt thyroid function. However, there is no clear evidence of PCB exposure disrupting thyroid hormone (TH) homeostasis in dogs and cats. The present study conducted in vivo experiments to evaluate the effects of a mixture of 12 PCB congeners (CB18, 28, 70, 77, 99, 101, 118, 138, 153, 180, 187 and 202, each congener 0.5 mg/kg BW, i.p. administration) on serum TH levels in male dogs (Canis lupus familiaris) and male cats (Felis silvestris catus). In PCB-exposed dogs, the time courses of higher-chlorinated PCBs and L-thyroxine (T4)-like OH-PCBs (4-OH-CB107 and 4-OH-CB202) concentrations were unchanged or tended to increase, whereas those of lower-chlorinated PCBs and OH-PCBs tended to decrease after 24 h. In PCB-exposed cats, concentrations of PCBs increased until 6 h and then remained unchanged. The levels of lower-chlorinated OH-PCBs including 4'-OH-CB18 increased until 96 h and then decreased. In PCB-exposed dogs, free T4 concentrations were higher than those in the control group at 48 and 96 h after PCB administration and positively correlated with the levels of T4-like OH-PCBs, suggesting competitive binding of T4 and T4-like OH-PCBs to a TH transporter, transthyretin. Serum levels of total T4 and total 3,3',5-triiodo-L-thyronine (T3) in PCB-exposed dogs were lower than in the control group at 24 and 48 h and negatively correlated with PCB concentrations, implying that PCB exposure enhanced TH excretion by increasing TH uptake and TH conjugation enzyme activities in the dog liver. In contrast, no obvious changes in TH levels were observed in PCB-exposed cats. This could be explained by the lower levels of T4-like OH-PCBs and lower hepatic conjugation enzyme activities in cats compared with dogs. Different effects on serum TH levels in PCB-exposed dogs and cats are likely to be attributable to species-specific PCB and TH metabolism.
• Age-related changes in bone morphology, function, and cell populations in inbred C57BL/6N mice
  Takashi Namba; Osamu Ichii; Teppei Nakamura; Md. Abdul Masu; Yuki Otani; Saori Otsuka-Kanazaw; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Jpn J Vet Res, 67, 4, 313, 317, Aug. 2019, [Peer-reviewed], [Corresponding author]
  English, Research institution
• Renal Infiltration of Macrophages in Canine and Feline Chronic Kidney Disease.
  Y Ohara; A Yabuki; R Nakamura; O Ichii; H Mizukawa; N Yokoyama; O Yamato
  Journal of comparative pathology, 170, 53, 59, Jul. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, During the progression of chronic kidney disease (CKD), macrophage infiltration is a crucial event leading to tubulointerstitial fibrosis. In the present study, macrophages infiltrating renal tissue in dogs and cats with CKD were analysed immunohistochemically. Iba-1 was used as a pan-macrophage marker, CD204 was used as a marker of M2 macrophages and tumour necrosis factor (TNF)-α was used as a marker of M1 macrophages. Signals for Iba1 and CD204 were observed in the interstitium of all tested kidney samples. In dogs, the signals were diffusely scattered. In cats, both diffuse and focal signals were observed. Cells that were positive for Iba1 and CD204 were also observed in the tubular lumina in cats. Co-expression of Iba1 and CD204 was also observed in the infiltrating cells by immunofluorescence labelling, and these cells were negative for TNF-α. By quantitative analysis, the indices for Iba1- and CD204-positive cells were significantly correlated with the concentrations of plasma creatinine and/or urea and the extent of interstitial fibrosis in both dogs and cats. These results demonstrated that renal infiltration of M2 macrophages plays an important role in the progression of CKD in dogs and cats. The distribution pattern of the kidney-infiltrating macrophages was unique in cats and may be associated with a cat-specific renal fibrotic process.
• Histopathological features of the proper gastric glands in FVB/N-background mice carrying constitutively-active aryl-hydrocarbon receptor.
  Ai Dantsuka; Osamu Ichii; Annika Hanberg; Yaser Hosny Ali Elewa; Saori Otsuka-Kanazawa; Teppei Nakamura; Yasuhiro Kon
  BMC gastroenterology, 19, 1, 102, 102, Springer Science and Business Media {LLC}, 21 Jun. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Aryl-hydrocarbon receptor (AhR) is a multiple ligand-activated transcription factor that has important roles in xenobiotic, physiological, or pathological functions. Transgenic mice systemically expressing constitutively-active AhR (CA-AhR) have been created to mimic activated AhR signaling in vivo. However, their detailed histopathological features are unclear. In the present study, we generated CA-AhR-expressing FVB/N mice (FVB-CA-AhR mice) and clarified their phenotypes in detail. METHODS: Male and female FVB-CA-AhR and wild-type mice were histopathologically examined from 6 to 33 weeks of age. RESULTS: Among the systemic organs, only the stomachs in FVB-CA-AhR mice showed pathological changes including cystic structures beneath the serosa; in addition, stomach weights increased with age. Histopathologically, cystic structures and alcian blue-positive metaplasia were observed in the mucosa of the proper gastric glands, and these two histometric parameters were positively correlated. Furthermore, proliferating cells shifted from the isthmus to the base of the glands, and parietal cells decreased. Age-related histopathological changes were clearer in females than in males. Importantly, in FVB-CA-AhR mice, intramucosal cysts developed as extramucosal cysts beneath the serosa, penetrating the lamina muscularis mucosae and the muscularis propria. Their incidence reached 100% in 28-week-old male mice and 33-week-old female mice. Extramucosal cysts contained alcian blue-, Griffonia simplicifolia lectin II-, or trefoil factor 2-positive cells, suggesting a stomach origin for the cysts and spasmolytic polypeptide-expressing metaplasia-like lesions. CONCLUSIONS: Disease onset occurred earlier in FVB-CA-AhR mice than previously reported in C57BL/6-derived CA-AhR mice. Importantly, the histopathological features were partly similar with gastritis cystica profunda in humans and animals. Excessive activation of AhR signaling aggravated abnormalities in the gastric mucosa and were affected by both genetic- and sex-related factors.
• Cotton Wool Sign in Paget Disease of Bone.
  Taro Horino; Osamu Ichii; Yoshio Terada
  Internal medicine (Tokyo, Japan), 58, 12, 1809, 1810, 15 Jun. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Overlapping Syndrome of Systemic Scleroderma and Cryoglobulinemic Vasculitis.
  Taro Horino; Kazu Hamada-Ode; Osamu Ichii; Yoshio Terada
  The American journal of medicine, 132, 6, e589-e590, Jun. 2019, [Peer-reviewed], [International Magazine]
  English
• A Rare Presentation of Hypermagnesemia Associated with Acute Kidney Injury due to Hypercalcemia.
  Taro Horino; Osamu Ichii; Yoshio Terada
  Internal medicine (Tokyo, Japan), 58, 8, 1123, 1126, 15 Apr. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, A 79-year-old Japanese woman presented with consciousness disturbance, hypercalcemia, and hypermagnesemia. She had rheumatoid arthritis and osteoporosis. Three months before admission, she was treated with oral calcitriol for osteoporosis. Two months before admission, she was treated with magnesium oxide for constipation. One month before admission, she underwent articular femoral bone replacement. Two weeks postoperatively, consciousness disturbance and elevated serum calcium levels were observed, and she was transferred to our hospital. On admission, her laboratory data showed elevated serum magnesium and creatinine levels. This is a rare case of coexistent hypercalcemia and hypermagnesemia associated with consciousness disturbance and acute kidney injury.
• Drug reaction with eosinophilia and systemic symptoms/drug-induced hypersensitivity syndrome (DRESS/DIHS) caused by levofloxacin in a patient with systemic scleroderma, rheumatoid arthritis, and Sjogren syndrome.
  Tatsuki Matsumoto; Taro Horino; Satoshi Inotani; Osamu Ichii; Yoshio Terada
  Contact dermatitis, 80, 4, 253, 254, Apr. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Spondyloarthropathy associated with glycogen storage disease type V mimicking polymyositis.
  T Horino; E Amano; T Furushima; O Ichii; Y Terada
  QJM : monthly journal of the Association of Physicians, 112, 4, 281, 282, 01 Apr. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• IgA-Proliferative Glomerulonephritis with Monoclonal Immunoglobulin Deposits.
  Taro Horino; Osamu Ichii; Yoshio Terada
  The American journal of medicine, 132, 4, e542-e545, Apr. 2019, [Peer-reviewed], [International Magazine]
  English
• Feature Article: Altered morpho-functional features of bones in autoimmune disease-prone BXSB/MpJ- Yaa mice.
  Takashi Namba; Osamu Ichii; Teppei Nakamura; Md Abdul Masum; Yuki Otani; Saori Otsuka-Kanazawa; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Experimental biology and medicine (Maywood, N.J.), 244, 5, 333, 343, {SAGE} Publications, Apr. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, IMPACT STATEMENT: Bone disease, such as osteoporosis and rheumatoid arthritis, increases because of the progression of an aging society. Autoimmune disease are important and predisposing factors for the pathogenesis of the bone disease; however, the pathological mechanism is unclear. We have demonstrated that systemic autoimmune disease in BXSB/MpJ- Yaa is closely associated with the morpho-functional abnormalities of bones including bone marrow and has complicated pathology. The abnormalities are characterized by altered regulations of serum calcium, anemia tendency, and hematopoiesis with increased WBCs and decreased PLs, short length and low mass of long bones, imbalance in the populations of osteoclasts and osteoblasts, and increased expression of candidate genes for causing and/or exacerbating their phenotypes. Therefore, BXSB/MpJ- Yaa serves as a model to elucidate bone phenotypes in systemic autoimmune disease that would be affected by the factors in the bone as well as the other immune and/or mineral metabolism organs both in human and experimental medicine.
• MRL/MpJ mice produce more oocytes and exhibit impaired fertilisation and accelerated luteinisation after superovulation treatment.
  Marina Hosotani; Osamu Ichii; Teppei Nakamura; Md Abdul Masum; Yuki Otani; Saori Otsuka-Kanazawa; Yaser H A Elewa; Yasuhiro Kon
  Reproduction, fertility, and development, 31, 4, 760, 773, Apr. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, MRL/MpJ mice exhibit distinct phenotypes in several biological processes, including wound healing. Herein we report two unique phenotypes in the female reproductive system of MRL/MpJ mice that affect ovulation and luteinisation. We found that superovulation treatment resulted in the production of significantly more oocytes in MRL/MpJ than C57BL/6 mice (71.0±13.4 vs 26.8±2.8 respectively). However, no exon mutations were detected in genes coding for female reproductive hormones or their receptors in MRL/MpJ mice. In addition, the fertilisation rate was lower for ovulated oocytes from MRL/MpJ than C57BL/6 mice, with most of the fertilised oocytes showing abnormal morphology, characterised by deformation and cytolysis. Histological tracing of luteinisation showed that MRL/MpJ mice formed corpora lutea within 36h after ovulation, whereas C57BL/6 mice were still at the corpora haemorrhagica formation stage after 36h. The balance between the expression of matrix metalloproteinases and their tissue inhibitors shifted towards the former earlier after ovulation in MRL/MpJ than C57BL/6 mice. This result indicates a possible link between accelerated extracellular matrix remodelling in the ovulated or ruptured follicles and luteinisation in MRL/MpJ mice. Together, these findings reveal novel phenotypes in MRL/MpJ mice that provide novel insights into reproductive biology.
• The intratumor heterogeneity of TP53 gene mutations in canine histiocytic sarcoma.
  Hajime Asada; Osamu Ichii; Hirotaka Tomiyasu; Kazuyuki Uchida; James K Chambers; Yuko Goto-Koshino; Koichi Ohno; Yasuhiro Kon; Hajime Tsujimoto
  The Journal of veterinary medical science, 81, 3, 353, 356, 14 Mar. 2019, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The mutations of TP53 gene are frequently observed in canine histiocytic sarcoma (HS). The objective of this study was to examine the distribution of tumor cells with TP53 gene mutations. Tumor tissues were divided into three or four regions and TP53 gene mutations were examined. TP53 gene mutations were detected only in parts of the HS tissues from six of the eight dogs, and the frequency of the mutant allele varied (0-65%) among the tumor regions. This study suggests that canine HS can exhibit intratumor heterogeneity. Further studies are needed to examine the clinical significance of the intratumor heterogeneity of TP53 gene mutations.
• Monoclonal Gammopathy of Undetermined Significance in a Patient With Synovitis, Acne, Pustulosis, Hyperostosis and Osteitis (SAPHO) Syndrome: Coincidence or Association?
  Taro Horino; Masami Ogasawara; Tatsuki Matsumoto; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 27, 8S, S557-S558, 12 Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Is recurrent Kikuchi-Fujimoto disease a precursor to systemic lupus erythematosus?
  Taro Horino; Osamu Ichii; Yoshio Terada
  Romanian journal of internal medicine = Revue roumaine de medecine interne, 57, 1, 72, 77, 01 Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Kikuchi-Fujimoto disease (KFD), also known as histiocytic necrotizing lymphadenitis, is a rare, benign, self-limiting disease characterized by cervical lymphadenopathy and fever. Since KFD was first reported in 1972, the validity of this clinical entity has been controversial and its aetiology remains unknown. Herein, we report a case of a patient with KFD, which was believed to be associated with systemic lupus erythematosus.
• Response to Makino: 'Hereditary spherocytosis presenting with branch retinal artery occlusion'.
  T Horino; O Ichii; T Asagiri; T Eguchi; Y Terada
  QJM : monthly journal of the Association of Physicians, 112, 3, 239, 240, 01 Mar. 2019, [Peer-reviewed], [International Magazine]
  English
• Food Yellow4 reprotoxicity in relation to localization of DMC1 and apoptosis in rat testes: Roles of royal jelly and cod liver oil.
  Yaser H A Elewa; Amany Abdel-Rahman Mohamed; Azza A A Galal; Nesma I El-Naseery; Osamu Ichii; Yasuhiro Kon
  Ecotoxicology and environmental safety, 169, 696, 706, Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Food Yellow 4 (FY4) is a lemon-yellow-colored synthetic organic azo dye, which is used widely for imparting pleasant and attractive appearance to foods and cosmetics. The present study aimed at evaluating the possible mechanism underlying the FY4-induced reprotoxicity in rats, and the potential supportive role of royal jelly (RJ) or cod liver oil (CLO), which is a natural remedy with several pharmacological benefits, against induced toxicity. Forty-eight male rats were divided into different groups-the control group, the CLO group (0.4 mL/kg), the RJ group (300 mg/kg), the FY4 group (500 mg/kg b.w.), and the co-treated groups (FY4 + CLO or FY4 + RJ). Semen analysis, serum hormones, and enzyme activities were estimated. Immunohistochemical staining was performed using anti-PCNA, anti-Sox 9, anti-STRA8, anti-DMC1, and anti-ssDNA antibody. The FY4 group exhibited a significant decrease in sperm concentration and motility percentage (%) and a substantial reduction in the TES and LH levels. Testicular LDH, ACP, and SDH were observed to be inhibited. Furthermore, co-localization of DMC1 and ssDNA, which reflected apoptotic induction in the leptotene and zygotene spermatocytes, respectively, was observed to have markedly elevated in the FY4 treated rats, with fewer PCNA-positive and SOX9-positive cells and higher ssDNA-positive cells in the seminiferous epithelium in comparison to the control groups. Interestingly, co-treatment with CLO or RJ exhibited healthy sperms and restored their features, activated the enzyme production, and raised the levels of sexual hormones. In addition, both RJ and CLO restored the features of the testicular tissue as observed under a light microscope, and limited the apoptosis as observed through antibody staining. Collectively, the results of the present study revealed that the co-administration of RJ or CLO with FY4 improved the biochemical, hormonal, and structural aspects of the testicular tissue in rats. Therefore, CLO and RJ may be considered promising agents that would be able to improve the testicular structure and function in the FY4-exposed individuals.
• Reactive Arthritis Caused by Haemophilus parainfluenzae in a Diabetic Patient.
  Tomohiro Eguchi; Taro Horino; Eri Amano; Osamu Ichii; Yoshio Terada
  The American journal of medicine, 132, 3, e519-e520, Mar. 2019, [Peer-reviewed], [International Magazine]
  English
• Induced expression of Toll-like receptor 9 in peritubular capillary endothelium correlates with the progression of tubulointerstitial lesions in autoimmune disease-prone mice.
  M A Masum; O Ichii; Y H A ELewa; Y Kon
  Lupus, 28, 3, 324, 333, Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Toll-like receptor (Tlr) 9 is capable of recognizing exogenous and/or endogenous nucleic acids and plays a crucial role in innate and adaptive immunity. Recently, we showed that Tlr9 is overexpressed in podocytes, a component of the blood-urine barrier (BUB), in glomeruli of autoimmune glomerulonephritis (AGN) model mice. This study investigated the activation of peritubular capillary (PTC) endothelial cells (ECs), a component of the BUB in the tubulointerstitium, through overexpressing Tlr9, and the subsequent development of tubulointerstitial lesions (TILs) in AGN model mice. METHODS: Lupus-prone BXSB/MpJ-Yaa (Yaa) and BXSB/MpJ (BXSB) mice were used as an AGN model and control, respectively. In addition to histopathological and ultrastructural techniques, protein and mRNA levels were also evaluated. The relationship between Tlr9 and TIL indices was analyzed by statistical correlation analysis. RESULTS: Yaa mice developed TILs and showed strong Tlr9 mRNA expression in PTC ECs at 24 weeks (wks) of age. However, BXSB mice showed no TIL but faint expression of Tlr9 mRNA at 8 and 24 wks of age. Tlr9 protein localization on PTC was almost absent in BXSB mice at both ages but intense expression was found in Yaa mice only at 24 wks of age. Relative mRNA expression of Tlr9 and its putative downstream cytokines, including interleukin 1 beta ( Il1b), Il6, interferon gamma ( Ifng), and tumor necrosis factor alpha ( Tnf) was markedly increased in isolated tubulointerstitium from Yaa mice at 24 wks of age. Furthermore, electron microscopy examination revealed PTC injury and TIL in Yaa mice at 24 wks. The expression level of Tlr9 in the tubulointerstitium was correlated with inflammatory cells in TILs, injured PTC, Ilb and Tnf expression, and damaged tubules ( P < 0.05 and 0.01). CONCLUSION: Induced expression of Tlr9 in ECs correlates with PTC injury and the development of TILs in lupus-prone AGN model mice.
• Cotton rat (Sigmodon hispidus) develops metabolic disorders associated with visceral adipose inflammation and fatty pancreas without obesity.
  Teppei Nakamura; Osamu Ichii; Takao Irie; Hirokazu Kouguchi; Kozue Sotozaki; Masataka Chihara; Yuji Sunden; Ken-Ichi Nagasaki; Osamu Tatsumi; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Cell and tissue research, 375, 2, 483, 492, Feb. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Obesity induces metabolic disorders such as type 2 diabetes, hypertension, and cardiovascular diseases and has become a global health concern. Recent studies imply that fat accumulation in nonadipose tissue correlates with metabolic disorders. However, there are no suitable animal models to evaluate this phenomenon. This study investigated the characteristics of metabolic disorders found in cotton rat (Sigmodon hispidus). Blood biochemical examinations revealed that cotton rats, predominantly males, developed hyperinsulinemia, hyperglycemia, and dyslipidemia when fed a normal diet. The islets increased in size through β-cell hyperplasia, which was associated with serum insulin level in both sexes, strongly indicating insulin resistance. In male cotton rats, oxidative stress was observed in β cells, and macrophage infiltration into the visceral white adipose tissue was reported, both of which were associated with serum insulin level without visceral obesity. In contrast, female cotton rats developed hyperinsulinemia without histopathological changes that were reported in males. Adipocytes were found to be accumulated in the pancreas but not in the liver of both sexes during aging. Pancreatic fat accumulation was associated with the serum insulin level only in females. Taken together, cotton rats developed metabolic disorders associated with visceral fat inflammation in the absence of obesity. In addition, pancreatic ectopic fat may also be related to the early stages of these conditions. Thus, the cotton rat may serve as a novel and useful model for metabolic disorders characterized by visceral adipose inflammation and ectopic fat accumulation in the pancreas without obesity.
• Relapsing Polychondritis Associated With Psoriasis Vulgaris.
  Taro Horino; Osamu Ichii; Tatsuki Matsumoto; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 04 Jan. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Intestinal Spirochetosis Caused by Brachyspira pilosicoli in a Systemic Lupus Erythematosus Patient
  Eguchi, T.; Inoue, K.; Horino, T.; Matsumoto, T.; Kamioka, S.; Nishida, Y.; Morimoto, M.; Morimoto, N.; Ichii, O.; Terada, Y.
  Journal of Clinical Rheumatology, 25, 8, 2019, [Peer-reviewed]
  Scientific journal
• Whole immunofluorescence staining of podocytes in fabry disease.
  Taro Horino; Toru Kubo; Osamu Ichii; Tatsuki Matsumoto; Kazu Hamada-Ode; Yoshio Terada
  Nephrology (Carlton, Vic.), 24, 1, 135, 135, Jan. 2019, [Peer-reviewed], [International Magazine]
  English
• Slc:Wistar/ST rats develop unilateral thyroid dysgenesis: A novel animal model of thyroid hemiagenesis.
  Teppei Nakamura; Osamu Ichii; Yuji Sunden; Yaser Hosny Ali Elewa; Tomoji Yoshiyasu; Hideki Hattori; Osamu Tatsumi; Yasuhiro Kon; Ken-Ichi Nagasaki
  PloS one, 14, 8, e0221939, 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Developmental anomalies of the thyroid gland lead to congenital malformations such as thyroglossal duct cysts and thyroid dysgenesis. However, the pathogenesis of thyroid dysgenesis remains unclear due to the lack of suitable animal models. This study demonstrated that Slc:Wistar/ST rats frequently developed unilateral thyroid dysgenesis, including hemiagenesis, characterized by the absence of one lobe. In Wistar/ST rats, each thyroid lobe was frequently different in size, and approximately 27% and 20% of the rats presented with hemihypoplasia and hemiagenesis of the thyroid gland, respectively. Dysgenesis was predominant on the left side in both sexes, without sex differences. At a young age, thyroid hemiagenesis did not alter body weight. In rats of both sexes with thyroid hemiagenesis, plasma total triiodothyronine and total triiodothyronine levels remained unchanged while plasma thyroid-stimulating hormone levels were significantly elevated in young rats. The remaining thyroid lobes increased in weight, but the follicular epithelial cells appeared normal in terms of their height and proliferating activities. On the side of thyroid dysgenesis, the parathyroid glands were normally localized and were situated at the same location as the contralateral glands. The ultimobranchial body remnants were localized at the level of the thyroid gland along with the cranial thyroid artery and vein, forming cell clusters or cystic structures and containing calcitonin-positive C-cells. In conclusion, Wistar/ST rats developed unilateral thyroid dysgenesis and may be novel and useful animal models for thyroid hemiagenesis in humans and for morphogenesis of pharyngeal pouch-derived organs.
• Corrigendum: Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats.
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in veterinary science, 6, 2, 2, 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, [This corrects the article DOI: 10.3389/fvets.2018.00289.].
• iBTA-induced bovine Biosheet for repair of abdominal wall defects in a beagle model: proof of concept.
  Y Nakayama; N Oshima; E Tatsumi; O Ichii; T Nishimura
  Hernia : the journal of hernias and abdominal wall surgery, 22, 6, 1033, 1039, Dec. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, INTRODUCTION: We evaluated the usefulness of xeno-Biosheets, an in-body tissue architecture-induced bovine collagenous sheet, as repair materials for abdominal wall defects in a beagle model. MATERIALS AND METHODS: Biosheets were prepared by embedding cylindrical molds into subcutaneous pouches of three Holstein cows for 2-3 months and stored in 70% ethanol. The Biosheets were 0.5 mm thick, cut into 2 cm × 2 cm, and implanted to replace defects of the same size in the abdominal wall of nine beagles. The abdominal wall and Biosheets were harvested and subjected to histological evaluation at 1, 3, and 5 months after implantation (n = 3 each). RESULTS: The Biosheet and bovine pericardiac patch (control) were not stressed during the suture operation and did not split, and patches were easily implanted on defective wounds. After implantation, the patch did not fall off and was not perforated, and healing was observed nacroscopically in all cases. During the first month of implantation, accumulation of inflammatory cells was observed along with decomposition around the Biosheet. Decomposition was almost complete after 3 months, and the Biosheet was replaced by autologous collagenous connective tissue without rejection. After 5 months, the abdominal wall muscle elongated from the periphery of the newly formed collagen layer and the peritoneum was formed on the peritoneal cavity surface. Regeneration of almost all layers of the abdominal wall was observed. However, almost all pericardium patches were remained even at 5 months with inflammation. CONCLUSION: Bovine Biosheets requiring no special post-treatment can be useful as off-the-shelf materials for abdominal wall repair.
• Hereditary spherocytosis presenting with branch retinal artery occlusion.
  T Horino; O Ichii; T Asagiri; T Eguchi; Y Terada
  QJM : monthly journal of the Association of Physicians, 111, 12, 899, 900, 01 Dec. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Bilateral striopallidodentate calcinosis associated with Sjögren's syndrome and IgD? monoclonal gammopathy of undetermined significance
  Taro Horino; Tatsuki Matsumoto; Kosuke Inoue; Osamu Ichii; Yoshio Terada
  Revue du Rhumatisme (Edition Francaise), 587, 590, Elsevier Masson SAS, 01 Dec. 2018
  French, Scientific journal
• Overexpression of toll-like receptor 9 correlates with podocyte injury in a murine model of autoimmune membranoproliferative glomerulonephritis.
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Teppei Nakamura; Yuki Otani; Marina Hosotani; Yasuhiro Kon
  Autoimmunity, 51, 8, 386, 398, Dec. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Toll-like receptors (Tlrs) are sensors of danger signals which promote the activation of immune cells and intrinsic renal cells. Podocytes, the intrinsic cells of glomerulus, are continuously exposed to various plasma solutes and danger signals due to their unique location in the glomerulus. Herein, we show that Tlr9 is overexpressed in podocytes and the mechanisms which cause its injury and development of membranoproliferative glomerulonephritis (MPGN) in model BXSB/MpJ-Yaa (Yaa) mice. Yaa mice developed typical lesions of MPGN and showed strong expression of Tlr9 mRNA throughout the glomerulus particularly toward the periphery of the glomerulus. However, BXSB/MpJ (BXSB) mice showed no lesion for MPGN but a very weak expression of Tlr9 mRNA. Relative mRNA expression of Tlr9 and its downstream cytokines, including interleukin 1 beta (Il1b), Il6, interferon gamma (Ifng) and tumour necrosis factor alpha (Tnfa) was markedly increased in glomeruli isolated from Yaa mice. Tlr9 protein expression was almost absent in BXSB mice but intense expression was found in Yaa mice. Podocyte protein expression was normal in BXSB mice but decreased in Yaa mice and colocalized with Tlr9 protein. Furthermore, electron microscopy examination revealed podocyte injury and electron-dense materials in thickened glomerular basement membrane of Yaa mice. Glomerular Tlr9 mRNA expression was significantly correlated with anti-dsDNA antibody, proteinuria, renal function indices (sBUN and sCr), glomerular histopathology indices, downstream factors of Tlr family (Ilb and Tnfa), podocyte injury parameters (p < .05 and p < .01). In conclusion, overexpression of TLR9 correlates with podocyte injury and development of MPGN.
• Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice.
  Md Atiqul Islam; Daisuke Torigoe; Yayoi Kameda; Takao Irie; Hirokazu Kouguchi; Ryo Nakao; Md Abdul Masum; Osamu Ichii; Yasuhiro Kon; Hassan T Tag-El-Din-Hassan; Masami Morimatsu; Kinpei Yagi; Takashi Agui
  Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 65, 65, 71, Nov. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically controlled. However, genetic factors responsible for these differences remain unknown. Our previous study in genetic linkage analysis has revealed that there is a significant quantitative trait locus (QTL) for the establishment of cyst (Emcys1), and a highly significant QTL for the development of protoscolex of E. multilocularis larvae (Empsc1), on mouse chromosomes 6 and 1, respectively. The current study aimed to confirm these QTLs and narrow down the critical genetic region that controls resistance/susceptibility to E. multilocularis infection by establishing congenic and subcongenic lines from C57BL/6 (B6) and DBA/2 (D2) mice. For protoscolex development phenotype, two congenic lines, B6.D2-Empsc1 and D2.B6-Empsc1 were developed, where responsible QTL, Empsc1 was introgressed from D2 into B6 background and vice versa. For cyst establishment phenotype, two congenic lines, B6.D2-Emcys1 and D2.B6-Emcys1 were developed, where responsible QTL, Emcys1 was introgressed from D2 into B6 background and vice versa. Because there was no significant difference in cyst establishment between B6.D2-Emcys1 and D2.B6-Emcys1 mice after challenge with E. multilocularis, it is suggested that the Emcys1 does not solely control the cyst establishment in mouse liver. However, infection experiments with B6.D2-Empsc1 and D2.B6-Empsc1 mice showed a significant difference in protoscolex development in the cyst. It confirms that the Empsc1 controls phenotype of the protoscolex development in the cyst. Subsequently, two subcongenic lines, B6.D2-Empsc1.1 and B6.D2-Empsc1.2 from B6.D2-Emcys1 and one subcongenic line, D2.B6-Empsc1.1 from D2.B6-Empsc1 were developed to narrow down the critical region responsible for protoscolex development. From the results of infection experiments with E. multilocularis in these subcongenic mice, it is concluded that a gene responsible for protoscolex development is located between D1Mit290 (68.1 cM) and D1Mit511 (97.3 cM).
• An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model.
  Nesma Ibraheim El-Naseery; Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon
  Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, 220, 9, 20, Nov. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The current study was conducted on a menopause rat model induced by ovariectomy to assess the histological and immunohistochemical alterations in the parotid glands and to verify the efficiency of human umbilical cord derived-mesenchymal stromal cell (hUCB-MSCs) in treating this condition. Eighteen adult female rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX) and OVX injected with hUCB-MSCs (OVX+hUCB-MSCs). At 3months post-ovariectomy, the salivary flow rate and size of the parotid glands were measured. The parotid glands were histologically investigated via H&E stained sections. Furthermore, immunohistochemical analysis for human CD105, human CD34, proliferating cell nuclear antigen (PCNA), single strand DNA (ssDNA), caspase 3, aquaporin (AQP)1, α-smooth muscle actin (α-SMA) and mouse CD34 were performed. The OVX group showed interstitial hemorrhage, dispersed acini and intracytoplasmic vacuoles in the acinar cells. Furthermore, immunohistochemical staining revealed a significant decrement in the number of ssDNA positive apoptotic cells, but a significant increment of PCNA positive proliferating cells, AQP1 positive blood capillaries, α-SMA positive myoepithelial cells and endogenous CD34 positive hematopoietic progenitor cells in the OVX+hUCB-MSCs group as compared with the OVX group. These findings suggest a potential regenerative therapy of MSCs to injured parotid gland structures. However, further investigations are required to illustrate the mechanism of hUCB-MSCs mediated parotid gland regeneration.
• Restricted localization of ultimobranchial body remnants and parafollicular cells in the one-humped camel (Camelus dromedarius).
  Teppei Nakamura; Yaser Hosny Ali Elewa; Osamu Ichii; Marina Hosotani; Wael A M Ghonimi; Osamu Tatsumi; Ken-Ichi Nagasaki; Yasuhiro Kon
  The Journal of veterinary medical science, 80, 9, 1368, 1372, 13 Sep. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Parafollicular cells (C-cells) exist within the thyroid glands and display different distributions within the glands among mammalian species. In the one-humped camel (Camelus dromedarius), localization of the C-cells remains under debate. We herein investigated appearance of C-cells and the remnants of the ultimobranchial body, origin of C-cells, in the thyroid glands of one-humped camels. Macroscopically, a white mass was present at one-third the length from the cranial end of the thyroid glands where the cranial thyroid artery entered. In addition, large fossae were frequently found adjacent to the white mass. Histologically, the mass was mainly composed of connective tissues, thyroid follicles, and two types of cell clusters: one was composed of cells with clear cytoplasm and the other was composed of non-keratinized epidermoid cells. The mass and the fossae contained p63-positive cells, indicating that they consisted of ultimobranchial body remnants. Calcitonin was expressed in cells with clear cytoplasm, which were localized just beneath the fossae and in the cell clusters of the white mass. C-cells also resided in both subfollicular and interfollicular spaces adjacent to the white mass, but gradually decreased toward the periphery. C-cells tended to display round shapes in the ultimobranchial body remnants and subfollicular spaces, and spindle shapes in interfollicular spaces. In conclusion, we demonstrated that the ultimobranchial body remnants were limited to the region around the entrance of cranial thyroid artery and vein, and C-cells were mainly concentrated within and around the ultimobranchial body remnants.
• Tissue-specific variation in 5'-terminal exons of mouse Anoctamin 1 transcript induces N-terminal variation of its protein via alternative translational start sites.
  Akihiro Kamikawa; Junpei Sakazaki; Osamu Ichii
  Biochemical and biophysical research communications, 503, 3, 1710, 1715, 10 Sep. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Anoctamin 1 (encoded by the Ano1 gene) is a Ca2+-activated Cl- channel critical to many physiological functions. It has been speculated that Ano1 expression is regulated in a tissue-dependent manner via alternative promoters. However, variation in the 5'-end sequence of mouse Ano1 (mAno1) and its tissue-dependent regulation are poorly understood. We identified a novel 5'-terminal exon (designated exon 1a) of mAno1 instead of the known 5'-terminal exon (exon 0) using 5'-rapid amplification of cDNA ends (RACE) analysis. Unexpectedly, the novel 5'-end variant mAno1Ex1a was abundantly expressed in many tissues including the salivary and mammary glands, rectum, lung, trachea and prostate. In contrast, the known variant mAno1Ex0 predominated only in male reproductive tissues such as the epididymis and testis. In a heterologous expression system, mAno1Ex0 encoded a longer protein than mAno1Ex1a, and this long isoform was abolished by a mutation in the exon 0 start codon. Moreover, the mAno1Ex0-specific N-terminal sequence was immunohistochemically detected in epididymis but not in salivary gland. Our data suggest that mAno1 expression is regulated via alternative promoters, and its transcriptional variation results in variation of the N-terminal sequence of the Ano1 protein due to the alternative translation initiation sites. These tissue-specific variations might contribute to the regulation of mAno1 expression and activity according to the physiological function of each tissue.
• Wall thickness control in biotubes prepared using type-C mold.
  Takeshi Terazawa; Takanori Nishimura; Tomohiro Mitani; Osamu Ichii; Teppei Ikeda; Keigo Kosenda; Eisuke Tatsumi; Yasuhide Nakayama
  Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs, 21, 3, 387, 391, Sep. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, A type-C mold based on in-body tissue architecture was previously developed for preparing small-diameter biotube vascular grafts with a 2-mm diameter and approximately 1-mm wall thickness. In this study, the type-C mold was modified for preparing large-diameter biotubes with controlled wall thicknesses. Four types of molds were assembled by inserting silicone center rods (outer diameters 11, 13, 15, 17 mm) into stainless steel cages (inner diameter 19 mm) and surgically embedded in the abdominal subcutaneous pouches of Holstein cows. After 8-12 weeks, connective tissues occupied the rod-cage gap in the molds to form biotubes. The wall thickness of the biotubes obtained after removing the molds was approximately 1-3 mm, which corresponded to approximately 80% of each gap distance. The breaking strength almost linearly increased with the wall thickness of the biotubes. The strength of the biotubes with wall thickness over 1.5 mm was higher than that of beagle blood vessels. The thickest biotubes were as strong as bovine pericardium and can be used as an alternative trachea graft because of their adequate lumen-holding force.
• Abnormal Morphology of Distal Tubular Epithelial Cells Is Regulated by Genetic Factors Derived from Mouse Chromosome 12.
  Osamu Ichii; Teppei Nakamura; Taro Horino; Akira Yabuki; Yaser H A Elewa; Yasuhiro Kon
  The American journal of pathology, 188, 9, 2120, 2138, Sep. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The distal tubule (DT) helps regulate blood pressure and electrolytes. We describe a novel, autosomal recessive, morphofunctional DT abnormality in inbred mice evident as columnar alternations and age-related cystic changes. This abnormality developed in both sexes of DBA/2Cr. Similar phenotypes were observed in A/J, C3H/He, DBA/1J, and FVB/N strains, but not in AKR/N, BALB/c, or C57BL/6N strains. In DBA/2Cr, abnormal DT localized to straight and convoluted segments and showed IL-36α DT injury marker expression. However, DT epithelial proliferation, examined by bromodeoxyuridine incorporation, was not remarkably altered with the progression of abnormality. Abnormal DT epithelial cells in DBA/2Cr displayed elongated primary cilia, loose intercellular adhesions, and numerous vesicles with altered localization of CD9, Na+/K+ATPase, and E-cadherin, indicating altered cell function, adhesion, and polarity. DBA/2Cr-type D12Mit182-D12Mit83 was identified as a candidate locus designated DBA/2 renal cyst (drecy). Within drecy, the gene regulated by estrogen in breast cancer protein (Greb1) transcript variant 2 was significantly up-regulated in DBA/2Cr kidney versus C57BL/6N. Greb1 localized to DT cytoplasm in C57BL/6 and to cytoplasm and nucleus in DBA/2Cr. Greb1-overexpressing M-1 kidney cells showed an altered epithelial-mesenchyme phenotype. B6.D2-(D12Mit182-D12Mit83) congenic mice carrying drecy did not show DT abnormalities, whereas DBA/2Cr × B6.D2-(D12Mit182-D12Mit83) mice did. Identification of this novel DT abnormality regulated by a DBA/2Cr mouse chromosome 12-derived locus and additional genetic factors improve the understanding of DT pathogenesis.
• Modified scanning electron microscopy reveals pathological crosstalk between endothelial cells and podocytes in a murine model of membranoproliferative glomerulonephritis.
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Teppei Nakamura; Yuki Otani; Marina Hosotani; Yasuhiro Kon
  Scientific reports, 8, 1, 10276, 10276, 06 Jul. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, This study evaluated endothelial cells and podocytes, both being primary components of the glomerular filtration barrier, in the progression of membranoproliferative glomerulonephritis (MPGN) using modified scanning electron microscopy (mSEM) analysis. BXSB/MpJ-Yaa model mice exhibited autoimmune-mediated MPGN characterised by elevated serum autoantibody levels, albuminuria, renal dysfunctional parameters, and decreased glomerular endothelial fenestrations (EF) and podocyte foot process (PFP) effacement with immune cell infiltration. Similar to transmission electron microscopy, mSEM revealed a series of pathological changes in basement membrane and densities of EF and PFP in BXSB/MpJ-Yaa compared with control BXSB/MpJ at different stages. Further, immunopositive area of endothelial marker (CD34), podocyte functional molecules (Nephrin, Podocin, Synaptopodin, and Wilms' tumour 1 (WT1)), and vascular endothelial growth factor A (VEGF A) significantly decreased in the glomerulus of BXSB/MpJ-Yaa compared with BXSB at final stage. The indices of glomerular endothelial injuries (EF density and immunopositive area of CD34 and VEGF A) and podocyte injuries (PEP density and immunopositive area of podocyte functional molecules) were also significantly correlated with each other and with indices of autoimmune disease and renal dysfunction. Thus, our results elucidated the pathological crosstalk between endothelial cells and podocytes in MPGN progression and the usefulness of mSEM for glomerular pathological analysis.
• Pulmonary hypertension due to unclassified interstitial lung disease in a Pembroke Welsh corgi.
  Tomoya Morita; Kensuke Nakamura; Tatsuyuki Osuga; Atsushi Kobayashi; Osamu Ichii; Akira Yabuki; Mitsuyoshi Takiguchi
  The Journal of veterinary medical science, 80, 6, 939, 944, 29 Jun. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, A 12 year-old intact male Pembroke Welsh corgi weighing 10.8 kg was presented for evaluation of a 3-month history of dyspnea, and a 1-week history of exercise intolerance and anorexia. Severe hypoxemia (PaO2 56 mmHg), diffuse lung alveolar infiltration, and severe pulmonary hypertension (PH) (tricuspid regurgitation pressure gradient was 81 mmHg) were identified. A tentative diagnosis of severe PH due to lung disease or pulmonary thromboembolism was made and treated intensively. After 5 days of hospitalization, the dog died despite oxygen supplementation and anticoagulant therapy. This dog was diagnosed as unclassified interstitial lung disease based on histopathological findings.
• Macrophage activation syndrome associated with systemic lupus erythematosus treated successfully with the combination of steroid pulse, immunoglobulin and tacrolimus.
  Natsuki Aoyama-Maeda; Taro Horino; Osamu Ichii; Yoshio Terada
  Romanian journal of internal medicine = Revue roumaine de medecine interne, 56, 2, 117, 121, 01 Jun. 2018, [Peer-reviewed], [International Magazine]
  English, Macrophage activation syndrome (MAS), a variant of secondary hemophagocyticlymphohistiocytosis, is a potentially life-threatening complication of inflammatory and autoimmune diseases. We present a case of MAS as a rare manifestation of systemic lupus erythematosus. Although initial treatment with corticosteroid, with or without cyclosporine A, is justified in patients with MAS, evidence regarding the effectiveness of this treatment protocol remains to be clarified. Our patient was successfully treated with a combination of intravenous immunoglobulin therapy and intravenous methyl predonisolone pulse therapy, which was followed by a course of oral prednisolone and oral tacrolimus. Based on our experience, we propose tacrolimus to provide a more useful adjuvant treatment to corticosteroid therapy than cyclosporine A.
• Cotton rats (Sigmodon hispidus) possess pharyngeal pouch remnants originating from different primordia.
  Teppei Nakamura; Osamu Ichii; Takao Irie; Tatsuya Mizoguchi; Akio Shinohara; Hirokazu Kouguchi; Yuji Sunden; Saori Otsuka-Kanazawa; Yaser Hosny Ali Elewa; Chihiro Koshimoto; Ken-Ichi Nagasaki; Yasuhiro Kon
  Histology and histopathology, 33, 6, 555, 565, Jun. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Pharyngeal pouches in mammals develop into specific derivatives. If the differentiation of the pharyngeal pouches is anomalous, their remnants can result in cysts, sinuses, and fistulae in the differentiated organs or around the neck. In the present study, we found several pharyngeal pouch remnants, such as cystic structures in thymus and parathyroid gland and fossulae extended from the piriform fossa, in the inbred cotton rats maintained at Hokkaido Institute of Public Health (HIS/Hiph) and University of Miyazaki (HIS/Mz). In HIS/Hiph, the fossulae extended from the apex of the piriform fossa into the thyroid glands and were lined with stratified squamous and cuboidal epithelium. Calcitonin-positive C-cells were present within their epithelium in HIS/Hiph. In contrast, the fossulae of HIS/Mz ran outside the thyroid glands toward the parathyroid glands; they were lined with columnar ciliated epithelium and a few goblet cells, but had no C-cells, which was consistent with the cystic structures in the thymus and the parathyroid gland. These results indicated that the fossulae were a remnant of the ultimobranchial body in HIS/Hiph and of the thymopharyngeal duct in HIS/Mz. Thus, the fossulae of the piriform fossa resembled the piriform sinus fistula in human. In conclusion, cotton rats frequently possessed pharyngeal pouch remnants, including the piriform sinus fistula, and therefore, might serve as a novel model to elucidate the mechanisms of pharyngeal pouch development.
• A case of acute kidney injury caused by granulomatous interstitial nephritis associated with sarcoidosis.
  Taro Horino; Tatsuki Matsumoto; Kosuke Inoue; Osamu Ichii; Yoshio Terada
  CEN case reports, 7, 1, 34, 38, May 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Sarcoidosis affects multiple organs including lung, heart and kidney. Sarcoidosis causes hypercalcemia, hypergammaglobulinemia, and rarely, granulomatous interstitial nephritis, resulting in renal stromal damage. Granulomatous interstitial nephritis is characterized as interstitial nephritis with noncaseating epithelioid granulomas. Diagnosing granulomatous interstitial nephritis before patient's death is challenging; hence, only few cases proven by renal biopsy have been reported till date. We present a case of acute kidney injury caused by granulomatous interstitial nephritis as a renal manifestation of sarcoidosis proven by renal biopsy, which can be confirmed by 18F-fluorodeoxyglucose positron emission tomography/computed tomography. Glucocorticoid therapy was helpful for improving and maintaining her renal function over a 6-year period.
• A glycomics approach to discover novel renal biomarkers in birds by administration of cisplatin and diclofenac to chickens.
  C Ishii; Y Ikenaka; O Ichii; S M M Nakayama; S-I Nishimura; T Ohashi; M Tanaka; H Mizukawa; M Ishizuka
  Poultry science, 97, 5, 1722, 1729, 01 May 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Avian species have a unique renal structure and abundant blood flow into the kidneys. Although many birds die due to nephrotoxicity caused by chemicals, there are no early biomarkers for renal lesions. Uric acid level in blood, which is generally used as a renal biomarker, is altered when the kidney function is damaged by over 70%. Therefore, early biomarkers for kidney injury in birds are needed. In humans, glycomics has been at the forefront of biological and medical sciences, and glycans are used as biomarkers of diseases, such as carcinoma. In this study, a glycomics approach was used to screen for renal biomarkers in chicken. First, a chicken model of kidney damage was generated by injection of diclofenac or cisplatin, which cause acute interstitial nephritis (AIN) and acute tubular necrosis (ATN), respectively. The nephrotoxicity levels were determined by a blood chemical test and histopathological analysis. The plasma N-glycans were then analyzed to discover renal biomarkers in birds. Levels of 14 glycans increased between pre- and post administration in kidney-damaged chickens in the diclofenac group, and some of these glycans had the same presumptive composition as those in human renal carcinoma patients. Glycan levels did not change remarkably in the cisplatin group. It is possible that there are changes in glycan expression due to AIN, but they do not reflect ATN. Although further research is needed in other species of birds, glycans are potentially useful biomarkers for AIN in avian species.
• Effects of topiroxostat in hyperuricemic patients with chronic kidney disease.
  Taro Horino; Yutaka Hatakeyama; Osamu Ichii; Tatsuki Matsumoto; Yoshiko Shimamura; Kosuke Inoue; Yoshio Terada; Yoshiyasu Okuhara
  Clinical and experimental nephrology, 22, 2, 337, 345, Apr. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, BACKGROUND: Hyperuricemia is associated with chronic kidney disease (CKD). Although topiroxostat, a novel, non-purine, selective xanthine oxidase inhibitor, has a strong effect against hyperuricemia, limited data are available on its renoprotective effect against CKD. METHODS: This study was conducted between October 2014 and May 2016. Thirty patients (20 male, 10 female) were administered 40 mg/day of topiroxostat twice daily. All patients were followed for a year. To elucidate the effects of topiroxostat, we evaluated the clinically documented primary indication of progression, viz. laboratory evidence of kidney function decline (reference indicator), uric acid, and hypertension in different patient groups, separated according to their baseline uProt levels and baseline eGFR. RESULTS: Topiroxostat treatment resulted in significant reduction in SUA (-1.53 mg/dL), systolic blood pressure (-8.9 mmHg), diastolic blood pressure (-5.0 mmHg), and urinary protein excretion (-795.5 mg/gCr) compared with baseline values. However, serum creatinine and urinary NAG levels, and estimated glomerular filtration rate did not change significantly. CONCLUSIONS: Topiroxostat reduced SUA levels effectively and may exhibit renoprotective effect in hyperuricemic patients with CKD. Further studies are required to clarify whether topiroxostat prevents the progression of renal disease and improves the prognosis of CKD patients.
• The First Case of Lupus Nephritis Developing in a Patient With Mantle Cell Lymphoma.
  Taro Horino; Yuki Osakabe; Mio Matsuura; Osamu Ichii; Yoshio Terada
  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, 24, 3, 159, 164, Apr. 2018, [Peer-reviewed], [International Magazine]
  English
• Renal thrombotic microangiopathy caused by bevacizumab.
  Taro Horino; Osamu Ichii; Yoshiko Shimamura; Yoshio Terada
  Nephrology (Carlton, Vic.), 23, 4, 378, 379, Apr. 2018, [Peer-reviewed], [International Magazine]
  English
• Changes in Renal Peritubular Capillaries in Canine and Feline Chronic Kidney Disease.
  R Nakamura; A Yabuki; O Ichii; H Mizukawa; N Yokoyama; O Yamato
  Journal of comparative pathology, 160, 79, 83, Apr. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Renal capillary rarefaction is a crucial event that leads to tubulointerstitial damage during the progression of chronic kidney disease (CKD). In the present study, changes in CD34-positive renal capillaries were investigated in dogs and cats with CKD. A significant decrease in CD34-positive capillaries was observed in canine diseased kidneys, even at the early stage of disease. In cats, CD34-positive capillaries were well preserved in the diseased kidneys, with no link to the severity of CKD. Renal capillary rarefaction might be a trigger event that leads to the progression of CKD in dogs, rather than in cats.
• Bilateral striopallidodentate calcinosis associated with Sjögren's syndrome and IgDλ monoclonal gammopathy of undetermined significance.
  Taro Horino; Tatsuki Matsumoto; Kosuke Inoue; Osamu Ichii; Yoshio Terada
  Joint bone spine, 85, 2, 243, 245, Mar. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, We presented the first case of bilateral striopallidodentate calcinosis secondary to Sjögren's syndrome. Further consideration should be given to the association between Sjögren's syndrome and bilateral striopallidodentate calcinosis, because Sjögren's syndrome is latent, but more frequent than other autoimmune diseases.
• Close pathological correlations between chronic kidney disease and reproductive organ-associated abnormalities in female cotton rats.
  Osamu Ichii; Teppei Nakamura; Takao Irie; Hirokazu Kouguchi; Kozue Sotozaki; Taro Horino; Yuji Sunden; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Experimental biology and medicine (Maywood, N.J.), 243, 5, 418, 427, Mar. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Cotton rat ( Sigmodon hispidus) is a useful experimental rodent for the study of human infectious diseases. We previously clarified that cotton rats, particularly females, developed chronic kidney disease characterized by cystic lesions, inflammation, and fibrosis. The present study investigated female-associated factors for chronic kidney disease development in cotton rats. Notably, female cotton rats developed separation of the pelvic symphysis and hypertrophy in the vaginal parts of the cervix with age, which strongly associated with pyometra. The development of pyometra closely associated with the deterioration of renal dysfunction or immunological abnormalities was indicated by blood urea nitrogen and serum creatinine or spleen weight and serum albumin/globulin ratio, respectively. These parameters for renal dysfunction and immunological abnormalities were statistically correlated. These phenotypes found in the female reproductive organs were completely inhibited by ovariectomy. Further, the female cotton rats with pyometra tended to show more severe chronic kidney disease phenotypes and immunological abnormalities than those without pyometra; these changes were inhibited in ovariectomized cotton rats. With regard to renal histopathology, cystic lesions, inflammation, and fibrosis were ameliorated by ovariectomy. Notably, the immunostaining intensity of estrogen receptor α and estrogen receptor β were weak in the healthy kidneys, but both estrogen receptors were strongly induced in the renal tubules showing cystic changes. In conclusion, the close correlations among female reproductive organ-associated abnormalities, immunological abnormalities, and renal dysfunction characterize the chronic kidney disease features of female cotton rats. Thus, the cotton rat is a unique rodent model to elucidate the pathological crosstalk between chronic kidney disease and sex-related factors. Impact statement The increasing number of elderly individuals in the overall population has led to a concomitant age-related increase in chronic kidney disease. Moreover, the global prevalence of patients with chronic kidney disease is gradually increasing, which poses a serious public health problem. The limited number of spontaneous chronic kidney disease animal models, which resemble chronic kidney disease pathogenesis in elderly individuals, is a major limitation in the development of experimental and curative medicines for chronic kidney disease. This pathological study clarified that sex-related factors, including hormones, and abnormalities of the female reproductive system, such as pyometra, are closely associated with chronic kidney disease development by using cotton rats ( Sigmodon hispidus). Further, ovariectomy inhibited the phenotypes of the female reproductive system, immunological abnormalities, and chronic kidney disease. Thus, this laboratory rodent serves as a novel and useful spontaneous chronic kidney disease model to elucidate the candidate disease factors and the pathogenesis of chronic kidney disease both in human and experimental medicine.
• A case of neuronal intranuclear inclusion disease associated with lupus nephritis-like nephropathy.
  Taro Horino; Tatsuki Matsumoto; Kosuke Inoue; Osamu Ichii; Yoshio Terada
  eNeurologicalSci, 10, 28, 30, Mar. 2018, [Peer-reviewed], [International Magazine]
  English, Neuronal intranuclear inclusion disease (NIID) is a relatively new entity identified as a progressive neurodegenerative disease characterised by eosinophilic hyaline intranuclear inclusions widely observed in neuronal and somatic cells. Renal biopsy from one of our patients with NIID showed lupus nephritis-like pathology. He was treated with steroids and angiotensin-converting enzyme inhibitors and his proteinuria improved. The present case highlights that immune-mediated glomerulonephritis can be a presenting feature of NIID, which can be controlled with proper treatment.
• Autoimmune abnormality affects ovulation and oocyte-pick-up in MRL/MpJ-Fas lpr/lpr mice.
  M Hosotani; O Ichii; T Nakamura; S O Kanazawa; Y Hosny Ali Elewa; Y Kon
  Lupus, 27, 1, 82, 94, Jan. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• MicroRNAs associated with the development of kidney diseases in humans and animals.
  Osamu Ichii; Taro Horino
  Journal of toxicologic pathology, 31, 1, 23, 34, Jan. 2018, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Mature microRNAs (miRNAs) are single-stranded RNAs with approximately 18-25 bases, and their sequences are highly conserved among animals. miRNAs act as posttranscriptional regulators by binding mRNAs, and their main function involves the degradation of their target mRNAs. Recent studies revealed altered expression of miRNAs in the kidneys during the progression of acute kidney injury (AKI) and chronic kidney disease (CKD) in humans and experimental rodent models by using high-throughput screening techniques including microarray and small RNA sequencing. Particularly, miR-21 seems to be strongly associated with renal pathogenesis both in the glomerulus and tubulointerstitium. Furthermore, abundant evidence has been gathered showing the involvement of miRNAs in renal fibrosis. Because of the complex morphofunctional organization of the mammalian kidneys, it is crucial both to determine the exact localization of the kidney cells that express the miRNAs, which has been addressed mainly using in situ hybridization methods, and to identify precisely which mRNAs are bound and degraded by these miRNAs, which has been studied mostly through in vitro analysis. To discover novel biomarker candidates, miRNA levels in urine supernatant, sediment, and exosomal fraction were comprehensively investigated in different types of kidney disease, including drug-induced AKI, ischemia-induced AKI, diabetic nephropathy, lupus nephritis, and IgA nephropathy. Recent studies also demonstrated the therapeutic effect of miRNA and/or anti-miRNA administrations. The intent of this review is to illustrate the state-of-the-art research in the field of miRNAs associated with renal pathogenesis, especially focusing on AKI and CKD in humans and animal models.
• Histopathological Correlations between Mediastinal Fat-Associated Lymphoid Clusters and the Development of Lung Inflammation and Fibrosis following Bleomycin Administration in Mice.
  Yaser Hosny Ali Elewa; Osamu Ichii; Kensuke Takada; Teppei Nakamura; Md Abdul Masum; Yasuhiro Kon
  Frontiers in immunology, 9, 271, 271, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Bleomycin (BLM) has been reported to induce lung inflammation and fibrosis in human and mice and showed genetic susceptibility. Interestingly, the C57BL/6 (B6) mice had prominent mediastinal fat-associated lymphoid cluster (MFALCs) under healthy condition, and showed susceptibility to development of lung fibrosis following BLM administration. However, the pathogenesis of lung lesion progression, and their correlation with MFALC morphologies, remain to be clarified. To investigate the correlations between MFALC structures and lung injuries in B6 mice, histopathological examination of mediastinal fat tissues and lungs was examined at 7 and 21 days (d) following a single 50 μL intranasal (i.n.) instillation of either BLM sulfate (5 mg/kg) (BLM group) or phosphate-buffered saline (control group). The lung fibrosis was examined by Masson's trichrome (MT) stain of paraffin sections and mRNA expression levels of Col1a1, Col3a1, and Acta2 in different frozen lung samples. Furthermore, immunohistochemistry for CD3, B220, Iba1, Gr1, BrdU, LYVE-1, and peripheral node addressin (PNAd) was performed to detect T- and B-cells, macrophages, granulocytes, proliferating cells, lymph vessels (LVs), and high endothelial venules (HEVs). We found that MFALCs were more abundant in the BLM group as compared to the control group. The lung of BLM group developed pneumonitis with severe cellular infiltrations at 7 days and significant collagen deposition (MT) and higher expression of Col1a1, and Col3a1 at 21 days post-administration. Numerous immune cells, proliferating cells, HEVs, and LVs were observed in both MFALCs and lungs of the BLM group. Interestingly, PNAd + HEVs were observed in the lungs of the BLM group, but not the control group. Moreover, numerous Gr1 + polymorphonuclear and mononuclear-like ring cells were found in the MFALCs and lungs of the BLM group. Interestingly, flow cytometric analysis revealed a significant increase of B-cell populations within the MFALCs of BLM group suggesting a potential proliferative induction of B-cells following inflammation. Furthermore, significant positive correlations were observed between quantitative parameters of these immune cells in both the lungs and MFALCs. Thus, we suggest a potentially important role for MFALCs and HEVs in the progression of lung disease, especially in inflammatory lung disease.
• Ovarian mast cells migrate toward ovary-fimbria connection in neonatal MRL/MpJ mice.
  Teppei Nakamura; Masataka Chihara; Osamu Ichii; Saori Otsuka-Kanazawa; Ken-Ichi Nagasaki; Yaser Hosny Ali Elewa; Osamu Tatsumi; Yasuhiro Kon
  PloS one, 13, 4, e0196364, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, MRL/MpJ mice have abundant ovarian mast cells (MCs) as compared with other strains at postnatal day 0 (P0); however, they sharply decrease after birth. These ovarian MCs, particularly beneath the ovarian surface epithelium (SE), which express mucosal MC (MMC) marker, might participate in early follicular development. This study investigated the changes in spatiotemporal distribution of MCs in the perinatal MRL/MpJ mouse ovaries. At P0 to P7, the MCs were densely localized to the ovary, especially their caudomedial region around the ovary-fimbria connection. The neonatal ovarian MCs showed intermediate characteristics of MMC and connective tissue MC (CTMC), and the latter phenotype became evident with aging. However, the expression ratio of the MMC to CTMC marker increased from P0 to P4 in the MRL/MpJ mouse ovary. Similarly, the ratio of MCs facing SE to total MC number increased with aging, although the number of ovarian MCs decreased, indicating the relative increase in MMC phenotypes in the early neonatal ovary. Neither proliferating nor apoptotic MCs were found in the MRL/MpJ mouse ovaries. The parenchymal cells surrounding MCs at ovary-fimbria connection showed similar molecular expression patterns (E-cadherin+/Foxl2-/Gata4+) as that of the ovarian surface epithelial cells. At P2, around the ovary-fimbria connection, c-kit- immature oocytes formed clusters called nests, and some MCs localized adjacent to c-kit- oocytes within the nests. These results indicated that in postnatal MRL/MpJ mice, ovarian MCs changed their distribution by migrating toward the parenchymal cells composing ovary-fimbria connection, which possessed similar characteristics to the ovarian surface epithelium. Thus, we elucidated the spatiotemporal alterations of the ovarian MCs in MRL/MpJ mice, and suggested their importance during the early follicular development by migrating toward the ovary-fimbria connection. MRL/MpJ mice would be useful to elucidate the relationship between neonatal immunity and reproductive systems.
• Hypereosinophilic syndrome manifested as eosinophilic gastroenteritis and colitis in a patient undergoing hemodialysis
  Amano, E.; Horino, T.; Ichii, O.; Inotani, S.; Matsumoto, T.; Hamada-Ode, K.; Shimamura, Y.; Inoue, K.; Terada, Y.
  Renal Replacement Therapy, 4, 1, 2018
  Scientific journal
• Morphofunctional analysis of antigen uptake mechanisms following sublingual immunotherapy with beads in mice.
  Yaser Hosny Ali Elewa; Tatsuya Mizoguchi; Osamu Ichii; Teppei Nakamura; Yasuhiro Kon
  PloS one, 13, 12, e0201330, Public Library of Science ({PLoS}), 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Recently, sublingual immunotherapy (SLIT) has been used as a safe and efficient method for the treatment of and immunization against asthma and various allergies. However, the routes of antigen/allergen (particulate antigen) uptake through the mucosa of the oral cavity remain incompletely understood, as do the roles of sex and age in the process. For this purpose, to elucidate the mechanism and efficacy of SLIT among different sexes and ages, microbeads were dripped into the sublingual region to mimic particulate antigen uptake by the sublingual mucosa. METHODS: Twenty microliters of either phosphate buffered saline (PBS) or fluorescently labelled microbeads (latex and silica beads) were placed under the tongue of both male and female C57BL/6 mice at young (3 months) and old (6 months) ages. The lower jaw was examined 30 min after administration, and beads were detected with a fluorescence stereomicroscope. Morphological observations of the mucosa of the fluorescent areas were made with a scanning electron microscope (SEM) and an all-in-one light fluorescence microscope (LM). Fluorescence intensity was compared between both sexes and ages. RESULTS: Stereomicroscopic observation revealed fluorescent illuminations in three compartments of the sublingual mucosa: the sublingual caruncles (SC), the oral rostral mucosa (OR) and the buccal mucosa (BM). Interestingly, the fluorescence intensity tended to be higher among females than among males in the SC region in particular. However, there were no significant age-related differences. SEM and LM revealed beads in the lumina of both mandibular ducts and sublingual ducts (Sd). Additionally, the apical cytoplasm of some Sd cells contained silica beads. However, there was no specification in the OR mucosa or BM. CONCLUSIONS: This study reveals the major role Sd plays in local immunity via the antigen uptake mechanisms. Furthermore, our data suggest that the efficacy of SLIT in humans could be affected by sex.
• Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats.
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in veterinary science, 5, 289, 289, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Increased incidence of kidney disease (KD) is a common concern in human and companion animals. Cats, in particular, are highly susceptible to KD. Novel KD biomarkers would help to address these problems. Therefore, we are focusing on microRNA, a highly conserved nucleic acid, as a KD biomarker for various animals. We previously reported that altered levels of urinary exosome (UExo)-derived microRNAs indicate renal pathologies in dogs. This study comprehensively examined UExo-derived microRNAs, which reflected the KD status in cats. The examined cats were divided into two groups: normal renal function (NR) and KD. Based on our previous data in dogs and cats, as well as the present data on UExo-derived microRNAs in cats by next-generation sequencing, let-7b, let-7f, miR-10a, miR-10b, miR-21a, miR-22, miR-26a, miR-27b, miR-146a, miR-181a, miR-191, and miR-486a were identified as biomarker candidates. In summary, the levels of UExo-derived let-7b, miR-22, and miR-26a significantly decreased in cats with KD from the early stages of the disease. UExo-derived miRNA levels normalized to urinary creatinine or total RNA of miR-21a was significantly higher in the KD group. Importantly, the ratio of UExo-derived miR-21a to let-7b showed a significant and strongest correlation with serum creatinine (ρ = 0.751), blood urea nitrogen (ρ = 0.754), and urinary creatinine (ρ = -0.421) among all examined indices. Further, the ratio of miR-181a to let-7b or miR-10b significantly correlated with the progression of renal dysfunction in the KD group. Thus, we identified that UExo-derived microRNAs in cats, and their raw and normalized levels could indicate altered renal function.
• First molecular detection of Sarcocystis ovalis in the intestinal mucosa of a Japanese jungle crow (Corvus macrorhynchos) in Hokkaido, Japan.
  Takao Irie; Tetsuya Ikeda; Teppei Nakamura; Osamu Ichii; Noriko Yamada; Takuya Ito; Akiko Yamazaki; Shinji Takai; Kinpei Yagi
  Veterinary parasitology, regional studies and reports, 10, 54, 57, Dec. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• PMab-52: Specific and Sensitive Monoclonal Antibody Against Cat Podoplanin for Immunohistochemistry.
  Shinji Yamada; Shunsuke Itai; Takuro Nakamura; Miyuki Yanaka; Noriko Saidoh; Yao-Wen Chang; Saori Handa; Hiroyuki Harada; Yumiko Kagawa; Osamu Ichii; Satoru Konnai; Mika K Kaneko; Yukinari Kato
  Monoclonal antibodies in immunodiagnosis and immunotherapy, 36, 5, 224, 230, Oct. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Podoplanin (PDPN) is expressed in several normal tissues, such as lymphatic endothelial cells, podocytes of renal glomerulus, and type I alveolar cells of lung. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. Although monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, rabbit PDPN, dog PDPN, and bovine PDPN have been established, anticat PDPN (cPDPN) mAbs have not been developed. In this study, we immunized mice with Chinese hamster ovary (CHO)-K1 cell lines expressing cPDPN, and developed anti-cPDPN mAbs. One of the clones, PMab-52 (IgM, kappa), detected cPDPN specifically in flow cytometry and Western blot analysis. PMab-52 is also useful for detecting feline squamous cell carcinoma cells in immunohistochemical analysis. PMab-52 is expected to be useful for investigating the function of cPDPN in feline carcinomas.
• Local CD34-positive capillaries decrease in mouse models of kidney disease associating with the severity of glomerular and tubulointerstitial lesions.
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Teppei Nakamura; Yasuhiro Kon
  BMC nephrology, 18, 1, 280, 280, 04 Sep. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Macromolecular creatine kinase type 1 in immune-mediated necrotizing myopathy.
  T Horino; O Ichii; K Ode-Hamada; Y Terada
  QJM : monthly journal of the Association of Physicians, 110, 9, 593, 594, 01 Sep. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Sex-related differences in autoimmune-induced lung lesions in MRL/MpJ-faslpr mice are mediated by the development of mediastinal fat-associated lymphoid clusters.
  Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon
  Autoimmunity, 50, 5, 306, 316, Aug. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Effects of a mixture of medetomidine, midazolam and butorphanol on anesthesia and blood biochemistry and the antagonizing action of atipamezole in hamsters.
  Teppei Nakamura; Naoya Karakida; Ai Dantsuka; Osamu Ichii; Yaser Hosny Ali Elewa; Yasuhiro Kon; Ken-Ichi Nagasaki; Hideki Hattori; Tomoji Yoshiyasu
  The Journal of veterinary medical science, 79, 7, 1230, 1235, 19 Jul. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Analysis of the mechanism of radiation-induced upregulation of mitochondrial abundance in mouse fibroblasts.
  Tohru Yamamori; Tomoya Sasagawa; Osamu Ichii; Mie Hiyoshi; Tomoki Bo; Hironobu Yasui; Yasuhiro Kon; Osamu Inanami
  Journal of radiation research, 58, 3, 292, 301, 01 May 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin.
  Shinji Yamada; Mika K Kaneko; Takuro Nakamura; Osamu Ichii; Satoru Konnai; Yukinari Kato
  Monoclonal antibodies in immunodiagnosis and immunotherapy, 36, 2, 77, 79, Apr. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb. Immunohistochemical analysis shows that mPMab-1 detects podocytes of the kidney, lymphatic endothelial cells of the colon, and type I alveolar cells of the lung. Importantly, mPMab-1 is more sensitive than PMab-1. This conversion strategy from rat mAb to mouse mAb could be applicable to other mAbs.
• Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11.
  Osamu Ichii; Masataka Chihara; Shin-Hyo Lee; Teppei Nakamura; Saori Otsuka-Kanazawa; Taro Horino; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Autoimmunity, 50, 2, 114, 124, Mar. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Localization of Toll-like Receptor (TLR) 2 and TLR4 mRNA in the Colorectal Mucosa of Miniature Dachshunds with Inflammatory Colorectal Polyps
  N. Yokoyama; H. Ohta; J. Yamazaki; Y. Kagawa; O. Ichii; N. Khoirun; T. Morita; T. Osuga; S. Y. Lim; N. Sasaki; K. Morishita; K. Nakamura; M. Takiguchi
  JOURNAL OF COMPARATIVE PATHOLOGY, 156, 2-3, 183, 190, Feb. 2017, [Peer-reviewed]
  English, Scientific journal
• Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs.
  Osamu Ichii; Hiroshi Ohta; Taro Horino; Teppei Nakamura; Marina Hosotani; Tatsuya Mizoguchi; Keitaro Morishita; Kensuke Nakamura; Yuki Hoshino; Satoshi Takagi; Noboru Sasaki; Mitsuyoshi Takiguchi; Ryo Sato; Kazuhisa Oyamada; Yasuhiro Kon
  Scientific reports, 7, 40340, 40340, 11 Jan. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• IL-36α Regulates Tubulointerstitial Inflammation in the Mouse Kidney.
  Osamu Ichii; Junpei Kimura; Tadashi Okamura; Taro Horino; Teppei Nakamura; Hayato Sasaki; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in immunology, 8, 1346, 1346, 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Lipoma arborescens in a patient with pseudogout on hemodialysis.
  T Horino; T Matsumoto; Y Terada; O Ichii
  QJM : monthly journal of the Association of Physicians, 109, 12, 827, 828, Dec. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Low expression of cyclooxygenase-2 in chronic kidney disease in young dogs.
  Akira Yabuki; Akiko Miyazaki; Osamu Ichii; Moeko Kohyama; Mariko Sawa; Osamu Yamato
  Research in veterinary science, 109, 71, 73, Dec. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Thyroid-like low-grade nasopharyngeal papillary adenocarcinoma: A case report.
  Taro Horino; Osamu Ichii; Kazu Hamada-Ode; Tatsuki Matsumoto; Yoshiko Shimamura; Kosuke Inoue; Yoshio Terada
  Molecular and clinical oncology, 5, 6, 693, 696, Dec. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Thyroid-like low-grade nasopharyngeal papillary adenocarcinoma (TL-LGNPPA) is a rare neoplasm characterized by morphological analogy to papillary thyroid carcinoma and abnormal expression of thyroid transcription factor-1 (TTF-1). We herein report a rare case of TL-LGNPPA with a review of its clinical, morphological and immunohistochemical characteristics. The patient was a 25-year-old Japanese woman complaining of a 2-year history of fever of unknown origin. There were no remarkable physical findings and the laboratory tests, including C-reactive protein levels, were normal. Laryngoscopy, magnetic resonance imaging and fluorodeoxyglucose-positron emission tomography identified a pedunculated mass at the roof of the nasopharynx. Histologically, the tumour exhibited papillary growth of cuboidal or columnar epithelium. Tubular architecture and a spindle cell component were also observed focally. Some tumour cells exhibited intranuclear cytoplasmic inclusions. Immunohistochemically, the neoplastic cells were positive for TTF-1, cytokeratin 7 and vimentin, but were negative for thyroglobulin. The Ki-67 labelling index (MIB-1 index) reached 5% in the most concentrated spot. The patient had neither local recurrence nor distant metastasis 3 years after removal of the tumour. In conclusion, TL-LGNPPA should be included it in the differential diagnosis of fever of unknown origin.
• Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse.
  Akihiro Kamikawa; Osamu Ichii; Junpei Sakazaki; Toru Ishikawa
  American journal of physiology. Cell physiology, 311, 5, C808-C819, C819, 01 Nov. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Does the routine handling affect the phenotype of disease model mice?
  Maiko Ono; Hayato Sasaki; Kenichi Nagasaki; Daisuke Torigoe; Osamu Ichii; Nobuya Sasaki; Takashi Agui
  The Japanese journal of veterinary research, 64, 4, 265, 271, Nov. 2016, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Usefulness of an anesthetic mixture of medetomidine, midazolam, and butorphanol in cotton rats (Sigmodn hispidus).
  Teppei Nakamura; Osamu Ichii; Takao Irie; Marina Hosotani; Ai Dantsuka; Saori Nakamura; Shinobu Sato; Kozue Sotozaki; Hirokazu Kouguchi; Tomoji Yoshiyasu; Ken-Ichi Nagasaki; Yasuhiro Kon
  The Japanese journal of veterinary research, 64, 4, 273, 276, Nov. 2016, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Degenerative and regenerative features of myofibers differ among skeletal muscles in a murine model of muscular dystrophy.
  Teppei Ikeda; Osamu Ichii; Saori Otsuka-Kanazawa; Teppei Nakamura; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Journal of muscle research and cell motility, 37, 4-5, 153, 164, Oct. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Rapid specimen preparation to improve the throughput of electron microscopic volume imaging for three-dimensional analyses of subcellular ultrastructures with serial block-face scanning electron microscopy.
  Truc Quynh Thai; Huy Bang Nguyen; Sei Saitoh; Bao Wu; Yurika Saitoh; Satoshi Shimo; Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon; Takashi Takaki; Kensuke Joh; Nobuhiko Ohno
  Medical molecular morphology, 49, 3, 154, 62, Sep. 2016, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes.
  Osamu Ichii; Teppei Nakamura; Takao Irie; Hirokazu Kouguchi; Daisuke Nakamura; Saori Nakamura; Shinobu Sato; Keisuke Yokoyama; Taro Horino; Yuji Sunden; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Histochemistry and cell biology, 146, 3, 351, 62, Sep. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Hydrogen sulfide activates TRPA1 and releases 5-HT from epithelioid cells of the chicken thoracic aorta.
  Dugar Delgermurun; Soichiro Yamaguchi; Osamu Ichii; Yasuhiro Kon; Shigeo Ito; Ken-Ichi Otsuguro
  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 187, 43, 9, Sep. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Spatiotemporal distribution of extracellular matrix changes during mouse duodenojejunal flexure formation.
  Sawa Onouchi; Osamu Ichii; Teppei Nakamura; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Cell and tissue research, 365, 2, 367, 79, Aug. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• MRL/MpJ mice show unique pathological features after experimental kidney injury.
  Daichi Shiozuru; Osamu Ichii; Junpei Kimura; Teppei Nakamura; Yaser Hosny Ali Elewa; Saori Otsuka-Kanazawa; Yasuhiro Kon
  Histology and histopathology, 31, 2, 189, 204, Feb. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Comparative analysis of mediastinal fat-associated lymphoid cluster development and lung cellular infiltration in murine autoimmune disease models and the corresponding normal control strains.
  Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon
  Immunology, 147, 1, 30, 40, Jan. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Genetic factors derived from the MRL/MpJ mouse function to maintain the integrity of spermatogenesis after heat exposure.
  M Chihara; T Nakamura; S Otsuka-Kanazawa; O Ichii; Y H A Elewa; Y Kon
  Andrology, 3, 5, 991, 9, Sep. 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Testicular oocytes in MRL/MpJ mice possess similar morphological, genetic, and functional characteristics to ovarian oocytes.
  Saori Otsuka-Kanazawa; Osamu Ichii; Yasuhiro Kon
  Mechanisms of development, 137, 23, 32, Aug. 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Asymmetric morphology of the cells comprising the inner and outer bending sides of the murine duodenojejunal flexure.
  Sawa Onouchi; Osamu Ichii; Saori Otsuka-Kanazawa; Yasuhiro Kon
  Cell and tissue research, 360, 2, 273, 85, May 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Morphological characteristics observed during early follicular development in perinatal MRL/MpJ mice.
  Yuma Yamashita; Teppei Nakamura; Saori Otsuka-Kanazawa; Osamu Ichii; Yasuhiro Kon
  The Japanese journal of veterinary research, 63, 1, 25, 36, Feb. 2015, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• MRL/MpJ-Fas(lpr) mice show abnormalities in ovarian function and morphology with the progression of autoimmune disease.
  Yuki Otani; Osamu Ichii; Saori Otsuka-Kanazawa; Masataka Chihara; Teppei Nakamura; Yasuhiro Kon
  Autoimmunity, 48, 6, 402, 11, 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Decrease in an Inwardly Rectifying Potassium Conductance in Mouse Mammary Secretory Cells after Forced Weaning.
  Akihiro Kamikawa; Shota Sugimoto; Osamu Ichii; Daisuke Kondoh
  PloS one, 10, 10, e0141131, 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Overexpression of Toll-like receptor 8 correlates with the progression of podocyte injury in murine autoimmune glomerulonephritis.
  Junpei Kimura; Osamu Ichii; Kosuke Miyazono; Teppei Nakamura; Taro Horino; Saori Otsuka-Kanazawa; Yasuhiro Kon
  Scientific reports, 4, 7290, 7290, 03 Dec. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Pathogenetic role of an autoimmune susceptibility locus derived from MRL/MpJ strain chromosome 1 in chronic pancreas inflammation.
  Y Okada; T Nakamura; O Ichii; S Otsuka; Y Kon
  Lupus, 23, 11, 1112, 23, Oct. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Characterization of mouse mediastinal fat-associated lymphoid clusters.
  Yaser Hosny Ali Elewa; Osamu Ichii; Saori Otsuka; Yoshiharu Hashimoto; Yasuhiro Kon
  Cell and tissue research, 357, 3, 731, 41, Sep. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• The onset of heat-induced testicular calcification in mice: involvement of the telomeric locus on chromosome 1.
  Masataka Chihara; Teppei Nakamura; Naoki Sakakibara; Saori Otsuka; Osamu Ichii; Yasuhiro Kon
  The American journal of pathology, 184, 9, 2480, 92, Sep. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Structural changes of goat parotid salivary gland: pre- and post-weaning periods.
  Y H A Elewa; O Ichii; S Otsuka; Y Hashimoto; Y Kon
  Anatomia, histologia, embryologia, 43, 4, 265, 72, Aug. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures.
  Minato Hirano; Kentaro Yoshii; Mizuki Sakai; Rie Hasebe; Osamu Ichii; Hiroaki Kariwa
  The Journal of general virology, 95, Pt 4, 849, 861, Apr. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• BXSB-type genome causes murine autoimmune glomerulonephritis: pathological correlation between telomeric region of chromosome 1 and Yaa
  J. Kimura; O. Ichii; T. Nakamura; T. Horino; S. Otsuka; Y. Kon
  GENES AND IMMUNITY, 15, 3, 182, 189, Apr. 2014, [Peer-reviewed]
  English, Scientific journal
• MicroRNA expression profiling of cat and dog kidneys.
  Osamu Ichii; Saori Otsuka; Hiroshi Ohta; Akira Yabuki; Taro Horino; Yasuhiro Kon
  Research in veterinary science, 96, 2, 299, 303, Apr. 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Genomic analysis of the appearance of ovarian mast cells in neonatal MRL/MpJ mice.
  Teppei Nakamura; Yuko Sakata; Saori Otsuka-Kanazawa; Osamu Ichii; Masataka Chihara; Ken-ichi Nagasaki; Yuka Namiki; Yasuhiro Kon
  PloS one, 9, 6, e100617, 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Podocyte injury caused by indoxyl sulfate, a uremic toxin and aryl-hydrocarbon receptor ligand.
  Osamu Ichii; Saori Otsuka-Kanazawa; Teppei Nakamura; Masaaki Ueno; Yasuhiro Kon; Weiping Chen; Avi Z Rosenberg; Jeffrey B Kopp
  PloS one, 9, 9, e108448, 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Decreased miR-26a expression correlates with the progression of podocyte injury in autoimmune glomerulonephritis.
  Osamu Ichii; Saori Otsuka-Kanazawa; Taro Horino; Junpei Kimura; Teppei Nakamura; Manabu Matsumoto; Makoto Toi; Yasuhiro Kon
  PloS one, 9, 10, e110383, 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Vitamin A deprivation affects the progression of the spermatogenic wave and initial formation of the blood-testis barrier, resulting in irreversible testicular degeneration in mice.
  Masataka Chihara; Saori Otsuka; Osamu Ichii; Yasuhiro Kon
  The Journal of reproduction and development, 59, 6, 525, 35, 17 Dec. 2013, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The blood testis-barrier (BTB) is essential for maintaining homeostasis in the seminiferous epithelium. Although many studies have reported that vitamin A (VA) is required for the maintenance of spermatogenesis, the relationships between the BTB, spermatogenesis and VA have not been elucidated. In this study, we analyzed BTB assembly and spermatogenesis in the testes of mice fed the VA-deficient (VAD) diet from the prepubertal period to adulthood. During the prepubertal period, no changes were observed in the initiation and progression of the first spermatogenic wave in mice fed the VAD diet. However, the numbers of preleptotene/leptotene spermatocytes derived from the second spermatogenic wave onwards were decreased, and initial BTB formation was also delayed, as evidenced by the decreased expression of mRNAs encoding BTB components and VA signaling molecules. From 60 days postpartum, mice fed the VAD diet exhibited apoptosis of germ cells, arrest of meiosis, disruption of the BTB, and dramatically decreased testis size. Furthermore, vacuolization and calcification were observed in the seminiferous epithelium of adult mice fed the VAD diet. Re-initiation of spermatogenesis by VA replenishment in adult mice fed the VAD diet rescued BTB assembly after when the second spermatogenic wave initiated from the arrested spermatogonia reached the preleptotene/leptotene spermatocytes. These results suggested that BTB integrity was regulated by VA metabolism with meiotic progression and that the impermeable BTB was required for persistent spermatogenesis rather than meiotic initiation. In conclusion, consumption of the VAD diet led to critical defects in spermatogenesis progression and altered the dynamics of BTB assembly.
• BXSB/MpJ-Yaa mice develop autoimmune dacryoadenitis with the appearance of inflammatory cell marker messenger RNAs in the lacrimal fluid.
  Keigo Kosenda; Osamu Ichii; Saori Otsuka; Yoshiharu Hashimoto; Yasuhiro Kon
  Clinical & experimental ophthalmology, 41, 8, 788, 97, Nov. 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Analysis of duodenojejunal flexure formation in mice: implications for understanding the genetic basis for gastrointestinal morphology in mammals.
  Sawa Onouchi; Osamu Ichii; Saori Otsuka; Yoshiharu Hashimoto; Yasuhiro Kon
  Journal of anatomy, 223, 4, 385, 98, Oct. 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes.
  Masataka Chihara; Ryoyo Ikebuchi; Saori Otsuka; Osamu Ichii; Yoshiharu Hashimoto; Atsushi Suzuki; Yumiko Saga; Yasuhiro Kon
  Biology of reproduction, 89, 1, 3, 3, Jul. 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Analysis of TdT-mediated dUTP nick end labeling (TUNEL)-positive cells associated with cardiac myogenesis in mouse embryo.
  Yuki Naganuma; Osamu Ichii; Saori Otsuka; Yoshiharu Hashimoto; Yasuhiro Kon
  The Journal of veterinary medical science, 75, 3, 283, 90, 2013, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Close relations between podocyte injuries and membranous proliferative glomerulonephritis in autoimmune murine models.
  Junpei Kimura; Osamu Ichii; Saori Otsuka; Hayato Sasaki; Yoshiharu Hashimoto; Yasuhiro Kon
  American journal of nephrology, 38, 1, 27, 38, 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Membranous proliferative glomerulonephritis (MPGN) is a major primary cause of chronic kidney disease (CKD). Podocyte injury is crucial in the pathogenesis of glomerular disease with proteinuria, leading to CKD. To assess podocyte injuries in MPGN, the pathological features of spontaneous murine models were analyzed. METHODS: The autoimmune-prone mice strains BXSB/MpJ-Yaa and B6.MRL-(D1Mit202-D1Mit403) were used as the MPGN models, and BXSB/MpJ-Yaa(+) and C57BL/6 were used as the respective controls. In addition to clinical parameters and glomerular histopathology, the protein and mRNA levels of podocyte functional markers were evaluated as indices for podocyte injuries. The relation between MPGN pathology and podocyte injuries was analyzed by statistical correlation. RESULTS: Both models developed MPGN with albuminuria and elevated serum anti-double-strand DNA (dsDNA) antibody levels. BXSB/MpJ-Yaa and B6.MRL showed severe proliferative lesions with T and B cell infiltrations and membranous lesions with T cell infiltrations, respectively. Foot process effacement and microvillus-like structure formation were observed ultrastructurally in the podocytes of both MPGN models. Furthermore, both MPGN models showed a decrease in immune-positive areas of nephrin, podocin and synaptopodin in the glomerulus, and in the mRNA expression of Nphs1, Nphs2, Synpo, Actn4, Cd2ap, and Podxl in the isolated glomerulus. Significant negative correlations were detected between serum anti-dsDNA antibody levels and glomerular Nphs1 expression, and between urinary albumin-to-creatinine ratio and glomerular expression of Nphs1, Synpo, Actn4, Cd2ap, or Podxl. CONCLUSION: MPGN models clearly developed podocyte injuries characterized by the decreased expression of podocyte functional markers with altered morphology. These data emphasized the importance of regulation of podocyte injuries in MPGN.
• Relationship between numerous mast cells and early follicular development in neonatal MRL/MpJ mouse ovaries.
  Teppei Nakamura; Saori Otsuka; Osamu Ichii; Yuko Sakata; Ken-Ichi Nagasaki; Yoshiharu Hashimoto; Yasuhiro Kon
  PloS one, 8, 10, e77246, 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Genomic analysis of the appearance of testicular oocytes in MRL/MpJ mice.
  Saori Otsuka; Osamu Ichii; Yuka Namiki; Nobuya Sasaki; Yoshiharu Hashimoto; Yasuhiro Kon
  Mammalian genome : official journal of the International Mammalian Genome Society, 23, 11-12, 741, 8, Dec. 2012, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in chickens infected with Marek's disease virus.
  Ayumi Matsuyama-Kato; Shiro Murata; Masayoshi Isezaki; Rika Kano; Sara Takasaki; Osamu Ichii; Satoru Konnai; Kazuhiko Ohashi
  Virology journal, 9, 94, 94, 21 May 2012, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Altered expression of microRNA miR-146a correlates with the development of chronic renal inflammation.
  Osamu Ichii; Saori Otsuka; Nobuya Sasaki; Yuka Namiki; Yoshiharu Hashimoto; Yasuhiro Kon
  Kidney international, 81, 3, 280, 92, Feb. 2012, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• The 129 genetic background affects susceptibility to glomerulosclerosis in tensin2-deficient mice.
  Tomohiro Nishino; Nobuya Sasaki; Ken-Ichi Nagasaki; Osamu Ichii; Yasuhiro Kon; Takashi Agui
  Biomedical research (Tokyo, Japan), 33, 1, 53, 6, Feb. 2012, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• A conserved region in the prM protein is a critical determinant in the assembly of flavivirus particles.
  Kentaro Yoshii; Manabu Igarashi; Osamu Ichii; Kana Yokozawa; Kimihito Ito; Hiroaki Kariwa; Ikuo Takashima
  The Journal of general virology, 93, Pt 1, 27, 38, Jan. 2012, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Infection of Hantaan virus strain AA57 leading to pulmonary disease in laboratory mice.
  Takahiro Seto; Noriyo Nagata; Keisuke Yoshikawa; Osamu Ichii; Takahiro Sanada; Ngonda Saasa; Yuka Ozaki; Yasunori Kon; Kentaro Yoshii; Ikuo Takashima; Hiroaki Kariwa
  Virus research, 163, 1, 284, 90, Jan. 2012, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Ovarian cysts in MRL / MpJ mice are derived from the extraovarian rete: a developmental study.
  Shin-Hyo Lee; Osamu Ichii; Saori Otsuka; Yaser Hosny Ali Elewa; Yuka Namiki; Yoshiharu Hashimoto; Yasuhiro Kon
  Journal of anatomy, 219, 6, 743, 55, Dec. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Pathological correlations between podocyte injuries and renal functions in canine and feline chronic kidney diseases.
  O Ichii; A Yabuki; N Sasaki; S Otsuka; H Ohta; M Yamasaki; M Takiguchi; Y Namiki; Y Hashimoto; D Endoh; Y Kon
  Histology and histopathology, 26, 10, 1243, 55, Oct. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Distinctive and critical roles for cellular immunity and immune-inflammatory response in the immunopathology of Sendai virus infection in mice.
  Ayo Yila Simon; Nobuya Sasaki; Osamu Ichii; Kiichi Kajino; Yasuhiro Kon; Takashi Agui
  Microbes and infection, 13, 8-9, 783, 97, Aug. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Identifying a new locus that regulates the development of rete ovarian cysts in MRL/MpJ mice.
  Shin-Hyo Lee; Osamu Ichii; Saori Otsuka; Yoshiharu Hashimoto; Yuka Namiki; Yasuhiro Kon
  The Japanese journal of veterinary research, 59, 2-3, 79, 88, Aug. 2011, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Hepatocyte nuclear factor 4 alpha is associated with mesenchymal-epithelial transition in developing kidneys of C57BL/6 mice.
  Tomonori Kanazawa; Osamu Ichii; Saori Otsuka; Yuka Namiki; Yoshiharu Hashimoto; Yasuhiro Kon
  The Journal of veterinary medical science, 73, 5, 601, 7, May 2011, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• High-resolution linkage mapping of the rat hooded locus.
  Daisuke Torigoe; Osamu Ichii; Ruihua Dang; Takayuki Ohnaka; Shinya Okano; Nobuya Sasaki; Yasuhiro Kon; Takashi Agui
  The Journal of veterinary medical science, 73, 5, 707, 10, May 2011, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Quantitative and qualitative urinary cellular patterns correlate with progression of murine glomerulonephritis.
  Junpei Kimura; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Yuka Namiki; Yoshiharu Hashimoto; Yasuhiro Kon
  PloS one, 6, 1, e16472, 31 Jan. 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Molecular pathology of murine ureteritis causing obstructive uropathy with hydronephrosis.
  Osamu Ichii; Saori Otsuka; Yuka Namiki; Yoshiharu Hashimoto; Yasuhiro Kon
  PloS one, 6, 11, e27783, 2011, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Primary causes of urinary tract obstruction that induces urine retention and results in hydronephrosis include uroliths, inflammation, and tumors. In this study, we analyzed the molecular pathology of ureteritis causing hydronephrosis in laboratory rodents.F2 progenies of C57BL/6 and DBA/2 mice were studied histopathologically and by comprehensive gene expression analysis of their ureters. Incidence of hydronephrosis was approximately 5% in F2 progenies. Histopathologically, this hydronephrosis was caused by stenosis of the proximal ureter, which showed fibrosis and papillary malformations of the proliferative epithelium with infiltrations of B-cell-dominated lymphocytes. Additionally, CD16-positive large granular leukocytes and eosinophils infiltrated from the ureteral mucosa to the muscular layer. Eosinophilic crystals were characteristically observed in the lumen of the ureter and the cytoplasm of large granular leukocytes, eosinophils, and transitional epithelial cells. Comprehensive gene profiling revealed remarkably elevated expression of genes associated with hyperimmune responses through activation of B cells in diseased ureters. Furthermore, diseased ureters showed dramatically higher gene expression of chitinase 3-like 3, known as Ym1, which is associated with formation both of adenomas in the transitional epithelium and of eosinophilic crystals in inflammatory conditions. The Ym1 protein was mainly localized to the cytoplasm of the transitional epithelium, infiltrated cells, and eosinophilic crystals in diseased ureters.We determined that the primary cause of hydronephrosis in F2 mice was ureteritis mediated by the local hyperimmune response with malformation of the transitional epithelium. Our data provide a novel molecular pathogenesis for elucidating causes of aseptic inflammation in human upper urinary tracts.
• Cytoarchitectural differences of myoepithelial cells among goat major salivary glands.
  Yaser Hosny Elewa; Mohammad Hafez Bareedy; Ahmed Awad Abuel-Atta; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Shin-Hyo Lee; Yoshiharu Hashimoto; Yasuhiro Kon
  Veterinary research communications, 34, 6, 557, 67, Aug. 2010, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Structural characteristics of goat (Capra hircus) parotid salivary glands.
  Yaser Hosny Elewa; Mohammad Hafez Bareedy; Ahmed Awad Abuel-Atta; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Shinhyo Lee; Yoshiharu Hashimoto; Yasuhiro Kon
  The Japanese journal of veterinary research, 58, 2, 121, 35, Aug. 2010, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Overexpression of interferon-activated gene 202 (Ifi202) correlates with the progression of autoimmune glomerulonephritis associated with the MRL chromosome 1.
  O Ichii; A Kamikawa; S Otsuka; Y Hashimoto; N Sasaki; D Endoh; Y Kon
  Lupus, 19, 8, 897, 905, Jul. 2010, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Molecular dynamics of the blood-testis barrier components during murine spermatogenesis.
  Masataka Chihara; Saori Otsuka; Osamu Ichii; Yoshiharu Hashimoto; Yasuhiro Kon
  Molecular reproduction and development, 77, 7, 630, 9, Jul. 2010, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Analysis of factors decreasing testis weight in MRL mice.
  Saori Otsuka; Yuka Namiki; Osamu Ichii; Yoshiharu Hashimoto; Nobuya Sasaki; Daiji Endoh; Yasuhiro Kon
  Mammalian genome : official journal of the International Mammalian Genome Society, 21, 3-4, 153, 61, Apr. 2010, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Quantitative trait locus analysis of ovarian cysts derived from rete ovarii in MRL/MpJ mice.
  Shin-hyo Lee; Osamu Ichii; Saori Otsuka; Yoshiharu Hashimoto; Yasuhiro Kon
  Mammalian genome : official journal of the International Mammalian Genome Society, 21, 3-4, 162, 71, Apr. 2010, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Local overexpression of interleukin-1 family, member 6 relates to the development of tubulointerstitial lesions.
  Osamu Ichii; Saori Otsuka; Nobuya Sasaki; Akira Yabuki; Hiroshi Ohta; Mitsuyoshi Takiguchi; Yoshiharu Hashimoto; Daiji Endoh; Yasuhiro Kon
  Laboratory investigation; a journal of technical methods and pathology, 90, 3, 459, 75, Mar. 2010, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Expression of caspase family and muscle- and apoptosis-specific genes during skeletal myogenesis in mouse embryo.
  Teppei Ikeda; Tomonori Kanazawa; Saori Otsuka; Osamu Ichii; Yoshiharu Hashimoto; Yasuhiro Kon
  The Journal of veterinary medical science, 71, 9, 1161, 8, Sep. 2009, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Diet-induced obesity disrupts ductal development in the mammary glands of nonpregnant mice.
  Akihiro Kamikawa; Osamu Ichii; Daisuke Yamaji; Takeshi Imao; Chiharu Suzuki; Yuko Okamatsu-Ogura; Akira Terao; Yasuhiro Kon; Kazuhiro Kimura
  Developmental dynamics : an official publication of the American Association of Anatomists, 238, 5, 1092, 9, May 2009, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Onset of autoimmune glomerulonephritis derived from the telomeric region of MRL-chromosome 1 is associated with the male sex hormone in mice.
  O Ichii; A Konno; N Sasaki; D Endoh; Y Hashimoto; Y Kon
  Lupus, 18, 6, 491, 500, May 2009, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Strain differences of cerebral ventricles in mice: can the MRL/MpJ mouse be a model for hydrocephalus?
  Keisuke Hino; Saori Otsuka; Osamu Ichii; Yoshiharu Hashimoto; Yasuhiro Kon
  The Japanese journal of veterinary research, 57, 1, 3, 11, May 2009, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Altered balance of inhibitory and active Fc gamma receptors in murine autoimmune glomerulonephritis.
  Osamu Ichii; Akihiro Konno; Nobuya Sasaki; Daiji Endoh; Yoshiharu Hashimoto; Yasuhiro Kon
  Kidney international, 74, 3, 339, 47, Aug. 2008, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Autoimmune glomerulonephritis induced in congenic mouse strain carrying telomeric region of chromosome 1 derived from MRL/MpJ.
  O Ichii; A Konno; N Sasaki; D Endoh; Y Hashimoto; Y Kon
  Histology and histopathology, 23, 4, 411, 22, Apr. 2008, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Immunohistochemical localization of renin, NO synthase-1, and cyclooxygenase-2 in rodent kidney.
  Osamu Ichii; Akira Yabuki; Toshimichi Ojima; Mitsuharu Matsumoto; Kazuyuki Taniguchi; Shusaku Suzuki
  Histology and histopathology, 23, 2, 143, 50, Feb. 2008, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Immunohistochemical localization of ghrelin in rodent kidneys.
  Akira Yabuki; Satoshi Taharaguchi; Osamu Ichii; Masayasu Kojima; Yoshihiro Nishi; Hiroharu Mifune; Ryozo Kamimura; Mitsuharu Matsumoto; Shusaku Suzuki
  Histochemistry and cell biology, 126, 2, 231, 8, Aug. 2006, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• 齧歯目の腎臓の組織構造における種差(Rodent Renal Structure Differs among Species)
  市居 修; 矢吹 映; 小嶋 敏慶; 松元 光春; 鈴木 秀作
  The Journal of Veterinary Medical Science, 68, 5, 439, 445, May 2006, [Peer-reviewed], [Domestic magazines]
  English

• 尿路上皮バリア異常モデルマウスにみられる急性腎障害
  市居修; 市居修; 岡村匡史; 大谷祐紀; 中村鉄平, 日本獣医学会学術集会講演要旨集, 167th, 2024
• コラーゲンの質を可視化する:変性コラーゲンと総コラーゲンのin situ多重染色法
  中村鉄平; 市居修; 市居修; 鷲見嘉奈子; 吉良俊之介; 森松正美; 森松正美, 日本獣医学会学術集会講演要旨集, 167th, 2024
• バイオデータベースより見出した新規低カルシウム血症モデルマウスの解析
  中村鉄平; 中村鉄平; 難波貴志; 市居修; 市居修; 市居修; 岩永理沙; 笹部一輝; 昆泰寛; 松村佳代子; 森松正美, 日本実験動物学会総会講演要旨集(Web), 71st, 2024
• 九州地域の野生獣肉における住肉胞子虫の保有状況
  渡部峻也; 入江隆夫; 入江隆夫; 和田啓; 中村鉄平; 中村鉄平; 市居修; 市居修; 市居修; 山崎朗子; 吉田彩子; 吉田彩子, 日本寄生虫学会大会プログラム・抄録集, 93rd, 2024
• 乳廃用牛の医療応用 皮下埋植した鋳型内の低酸素環境は結合組織体の形成を誘導する
  中村 鉄平; 市居 修; 柳瀬 圭太; 池田 哲平; 平石 真也; 片山 由佳; 海田 こころ; 新川 祐希; 小川 美朱; 辻 絵里香; 秋吉 秀保; 森松 正美; 西邑 隆徳, 日本バイオマテリアル学会大会予稿集, 45回, 155, 155, Oct. 2023
  日本バイオマテリアル学会, Japanese
• 乳廃用牛の医療応用 生体内で形成された結合組織体の機械特性評価
  柳瀬 圭太; 市居 修; 中村 鉄平; 海田 こころ; 片山 由佳; 池田 哲平; 平石 真也; 新川 祐希; 小川 美朱; 秋吉 秀保; 西邑 隆徳, 日本バイオマテリアル学会大会予稿集, 45回, 158, 158, Oct. 2023
  日本バイオマテリアル学会, Japanese
• MRL/MpJ-FasIpr/Iprマウスの直腸リンパ組織を構成する免疫細胞(Immune cells constituting the rectal lymphoid tissues in MRL/MpJ-FasIpr/Ipr mice)
  Uddin Rubel Md. Zahir; 市居 修; 難波 貴志; Abdul Masum Md.; 平石 真也; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 166回, 90, 90, Sep. 2023
  (公社)日本獣医学会, English
• 初回人工授精前の子宮内膜組織を用いた乳牛の低受胎に関わる子宮内細菌叢の解析
  八木沢 拓也; 内山 淳平; 内山 伊代; 安藤 駿; 市居 修; 村上 裕信; 松下 治; 片桐 成二, 日本細菌学雑誌, 78, 1, 72, 72, Feb. 2023
  日本細菌学会, Japanese
• 生殖内分泌学と生殖免疫学の接点 種横断的な知見から考える精巣と免疫のかかわり
  大谷祐紀; 大谷祐紀; 市居修; 昆泰寛, Hormone Frontier in Gynecology, 30, 3, 209, 216, 2023
  大阪 : メディカルレビュー社, Japanese
• 自己免疫疾患モデルマウス頭部にみられる粘膜上皮-免疫連関の変化
  平石真也; 市居修; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 166th, 93, 93, 2023
  (公社)日本獣医学会, Japanese
• 薬物誘導型妊娠糖尿病モデルマウスの作出とその病態について
  高橋なるみ; 市居修; 大谷祐紀; 難波貴志; 平石真也; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 166th, 90, 90, 2023
  (公社)日本獣医学会, Japanese
• Roles of an inflammatory cytokine TL1A in gdT cell activation and psoriasis pathogenesis
  王上一; 香西美奈; RUBEL Md. Zahir Uddin; 平石真也; 市居修; 高田健介; 稲葉睦, 日本獣医学会学術集会講演要旨集, 166th, 121, 121, 2023
  (公社)日本獣医学会, English
• Immune cells constituting the rectal lymphoid tissues in MRL/MpJ-Fas^lpr/lpr mice
  UDDIN Rubel Md. Zahir; UDDIN Rubel Md. Zahir; 市居修; 難波貴志; ABDUL Masum Md.; 平石真也; 昆泰寛, 日本獣医学会学術集会講演要旨集, 166th, 2023
• 動物の腎疾患における尿中エクソソーム由来マイクロRNAの動態
  市居修; 市居修, 日本獣医学会学術集会講演要旨集, 166th, 170, 170, 2023
  (公社)日本獣医学会, Japanese
• コットンラットの胸腺にみられる嚢胞の由来と胸腺機能との関連
  中村鉄平; 市居修; 入江隆夫; 篠原明男; 孝口裕一; 昆泰寛; 森松正美, 日本獣医学会学術集会講演要旨集, 166th, 208, 208, 2023
  (公社)日本獣医学会, Japanese
• Epidemiology of Sarcocystis spp. in wild game meat in Kyushu, Japan and evaluation of their pathogenicity
  渡部峻也; 中村鉄平; 市居修; 山崎朗子; 吉田彩子; 入江隆夫, 日本寄生虫学会大会プログラム・抄録集, 92nd, 2023
• Medical Application of Dairy Waste Cattle-Hypoxic Condition Induces Dense Connective Tissue Formation in Subcutaneously Implanted Molds.
  中村鉄平; 中村鉄平; 市居修; 市居修; 柳瀬圭太; 柳瀬圭太; 池田哲平; 平石真也; 片山由佳; 海田こころ; 新川祐希; 小川美朱; 辻絵里香; 秋吉秀保; 森松正美; 西邑隆徳, 日本バイオマテリアル学会大会予稿集(Web), 45th, 155, 155, 2023
  日本バイオマテリアル学会, Japanese
• Evaluation of Mechanical Properties of Connective Tissue Prepared in Dairy Waste Cattle for Medical Application.
  柳瀬圭太; 市居修; 市居修; 中村鉄平; 中村鉄平; 海田こころ; 片山由佳; 池田哲平; 平石真也; 新川祐希; 小川美朱; 秋吉秀保; 西邑隆徳, 日本バイオマテリアル学会大会予稿集(Web), 45th, 158, 158, 2023
  日本バイオマテリアル学会, Japanese
• 乳牛における子宮内細菌叢と受胎性との関連についての検討
  八木沢拓也; 内山淳平; 内山伊代; 安藤駿; 市居修; 村上裕信; 松下治; 片桐成二, 北海道獣医師会雑誌, 67, 8, 294, 294, 2023
  (公社)北海道獣医師会, Japanese
• Novel polychrome staining distinguishing osteochondral tissue and bone cells [JFRL staining]: a beneficial tool for biological safety evaluation in medical devices.
  辻絵里香; 鷲見嘉奈子; 中村鉄平; 近藤大輔; 辻尾祐志; 細谷実里奈; 難波貴志; 脇谷晶一; 市居修; 市居修; 西野光一郎; 昆泰寛; 三嶋隆; 吉安友二; 勝田真一, 日本バイオマテリアル学会大会予稿集(Web), 44回, 415, 415, Nov. 2022
  日本バイオマテリアル学会, Japanese
• REV-ERBアゴニストはγδT17細胞を抑制しマウスモデルの乾癬性皮膚炎を改善する(REV-ERB agonist suppresses γδ T17 cells and improves psoriatic dermatitis in a mouse model)
  王 上一; 香西 美奈; 三田 泰誠; Cai Zimeng; Abdul Masum Md.; 市居 修; 高田 健介; 稲葉 睦, 日本獣医学会学術集会講演要旨集, 165回, [DI1A, 07], Sep. 2022
  (公社)日本獣医学会, English
• コロナ禍及びこれからの獣医解剖学実習 解剖実習教材の変遷から考察した獣医学教育
  市居 修; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A2A, 04], Sep. 2022
  (公社)日本獣医学会, Japanese
• 乳用種廃用牛を利用した移植用結合組織体の作製 スリット形状が与える影響
  片山 由佳; 市居 修; 柳瀬 圭太; 中村 鉄平; 池田 哲平; 難波 貴志; 大谷 祐紀; 昆 泰寛; 西邑 隆徳, 日本獣医学会学術集会講演要旨集, 165回, [A2P, 01], Sep. 2022
  (公社)日本獣医学会, Japanese
• 尿管閉塞モデルマウスの尿管移行上皮に誘導される17型コラーゲンの発現動態
  難波 貴志; 市居 修; 中村 鉄平; 大谷 祐紀; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A2P, 04], Sep. 2022
  (公社)日本獣医学会, Japanese
• マウス急性腎障害期尿の尿路上皮バリアと免疫への作用
  市居 修; 難波 貴志; 大谷 祐紀; Elewa Yaser Hosny Ali; 中村 鉄平; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A2P, 05], Sep. 2022
  (公社)日本獣医学会, Japanese
• 全身性自己免疫異常がマウス骨格筋の外傷性損傷とその再生に与える影響について
  馬杉 実里; 市居 修; 大谷 祐紀; 難波 貴志; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A2P, 07], Sep. 2022
  (公社)日本獣医学会, Japanese
• マウス肛門直腸粘膜に見出されたリンパ組織とその形態に影響を及ぼす全身性因子(Lymphoid tissues found in mouse anorectal mucosa and systemic factors affecting their morphology)
  Rubel Md Zahir Uddin; 市居 修; 難波 貴志; Masum Md. Abdul; Chuluunbaata Tsolmon; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A2P, 11], Sep. 2022
  (公社)日本獣医学会, English
• 肺炎罹患牛における結膜関連リンパ組織(CALT)の特徴
  小千田 圭吾; 市居 修; 大塚 浩通; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A2P, 12], Sep. 2022
  (公社)日本獣医学会, Japanese
• BXSB/MpJ-Yaaマウスの病態からみる全身免疫と頭部粘膜免疫の関連
  平石 真也; 市居 修; 中村 鉄平; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A2P, 13], Sep. 2022
  (公社)日本獣医学会, Japanese
• ヤギおよびブタの腟前庭にみられた生殖器関連リンパ組織(Genital organ associated lymphoid tissues found in the vaginal vestibule of goats and pigs)
  Chuluunbaatar Tsolmon; 市居 修; 難波 貴志; Masum Md. Abdul; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A3A, 04], Sep. 2022
  (公社)日本獣医学会, English
• 妊娠期自己免疫疾患モデルマウスの胎盤と妊孕性の関連
  山中 里紗; 市居 修; 中村 鉄平; 大谷 祐紀; 難波 貴志; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A3P, 06], Sep. 2022
  (公社)日本獣医学会, Japanese
• コットンラットの系統差比較が解き明かすヒト下咽頭梨状窩瘻の由来
  中村 鉄平; 細谷 実里奈; 市居 修; 渡邉 敬文; Elewa Yaser Hosny Ali; 入江 隆夫; 篠原 明男; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 165回, [A3P, 10], Sep. 2022
  (公社)日本獣医学会, Japanese
• ワクモ(Dermanyssus gallinae)由来シスタチン様分子の抗ワクモワクチン抗原としての評価
  藤澤 宗太郎; 村田 史郎; 伊勢崎 政美; 有泉 拓馬; 佐藤 匠; 大石 英司; 種子野 章; 前川 直也; 岡川 朋弘; 市居 修; 今内 覚; 大橋 和彦, 日本獣医学会学術集会講演要旨集, 165回, [E3P, 01], Sep. 2022
  (公社)日本獣医学会, Japanese
• ワクモ由来の2種類のアスパラギン酸ペプチダーゼの性状解析
  瀬尾 光里; 村田 史郎; Shwe Yee Win; 藤澤 宗太郎; 北條 巧; 伊勢崎 政美; 佐藤 匠; 大石 英司; 種子野 章; 市居 修; 前川 直也; 岡川 朋弘; 今内 覚; 大橋 和彦, 日本獣医学会学術集会講演要旨集, 165回, [E3P, 03], Sep. 2022
  (公社)日本獣医学会, Japanese
• Kidney and its pathology in dog and cat.
  市居修; 市居修, 月刊腎臓内科, 16, 2, 186, 194, Aug. 2022
  (有)科学評論社, Japanese
• 九州産ジビエの住肉胞子虫の保有状況と流行種
  渡部峻也; 中村鉄平; 市居修; 吉田彩子; 吉田彩子; 入江隆夫; 入江隆夫, 日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州), 2022 (CD-ROM), 2022
• 腎臓の発生
  市居修, 日本先天異常学会学術集会プログラム・抄録集, 62nd (CD-ROM), 2022
• マダニ由来培養細胞に持続感染している新規オルビウイルス
  松野啓太; 田谷友里恵; 田谷友里恵; 高木善弘; 市居修; 平井美穂; 中尾亮; 布浦拓郎; 松尾栄子, 日本ウイルス学会学術集会プログラム・予稿集(Web), 69th, 2022
• 非典型的な病理組織像を呈した糸球体腎症の犬の1例
  西田暁子; 大田寛; 上沢広道; 難波貴志; 市居修; 長谷川寧々; 酒井俊和; 鳥巣至道; 出口辰弥; 田村昌大; 山崎裕毅, 北海道獣医師会雑誌, 66, 8, 290, 290, 2022
  (公社)北海道獣医師会, Japanese
• Unique Roles of Urine: Biological Fluid Alerting Disease Progression and Altering Local Immune Status in the Kidney of Animals
  Osamu Ichii, Proceedings for Annual Meeting of The Japanese Pharmacological Society, 95, 1-S01-1, 2022
  The number of human patients with chronic kidney disease (CKD) is increasing with the aging of society. Similarly, the incidence of CKD in animals, especially dogs and cats, is also increasing with the aging of individuals.
  
  In order to achieve "One Health" for humans and animals, it is important to study useful targets for the diagnosis and treatment of CKD in these mammals. However, the species specific-differences of renal pathogenesis complicate it; dogs tend to show renal glomerular lesions as humans, but most cats manifest tubulointerstitial lesions. Therefore, there is a need to develop therapeutic and diagnostic methods based on the elucidation of species-specific renal pathogenesis, and we are conducting comprehensive research targeting human as well as model animals and companion animals.
  
  In particular, we continue to focus on the unique role of "urine" in various kidney diseases. Our previous studies have shown that urine from model or companion animals with CKD contains candidate disease markers such as cytokines and non-coding RNAs, which are involved in their renal pathogenesis. In addition, as for a unique recent topic, we found that urine is a biological fluid that alters the local immune status of urogenital tissues. Briefly, tertiary lymphoid structures in the renal pelvis, called "urinary tract-associated lymphoid structures (UTALSs)", were formed in humans and mice with chronic nephritis, regardless of infections. In the development of UTALSs, aging and altered immune status as wells as urine played a role in UTALS formation. In a nephritis mouse model, urine leaked from the lumen of the renal pelvis into its parenchyma. Based on obtained findings, we consider that altered urine-urothelium barrier-based UTALS formation may represent a novel mechanism underlying the pathogenesis of chronic nephritis, regardless of urinary tract infection.
  
  Further studies focusing on urinary biomarker candidates and unique roles of urine in renal pathogenesis would be crucial for clinical development and understanding the mechanism of kidney disease progression, to realize One Health in the field of human and animal nephrology., Japanese Pharmacological Society, Japanese
• ヤギ腎臓における血管系リンパ組織の分類(Clarification of vasculature-associated lymphoid tissue in goat kidney)
  Masum Md. Abdul; 市居 修; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 25], Sep. 2021
  (公社)日本獣医学会, English
• マウス卵管線毛上皮細胞と排卵卵子の相互作用 加齢と免疫異常は卵子輸送速度を変化させる
  細谷 実里奈; 市居 修; 渡邉 敬文; 植田 弘美; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 37], Sep. 2021
  (公社)日本獣医学会, Japanese
• 牛の腟前庭にみられたリンパ陰部輪(Genital Lymphoid Ring found in the vaginal vestibule of cow)
  Chuluunbaatar Tsolmon; 市居 修; Masum Md. Abdul; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 38], Sep. 2021
  (公社)日本獣医学会, English
• シチメンチョウの気管上皮に発現する多様な鳥インフルエンザウイルスレセプターの構造解析
  小林 大樹; 市居 修; 日尾野 隆大; 西原 祥子; 高瀬 明; 磯田 典和; 迫田 義博, 日本獣医学会学術集会講演要旨集, 164回, [DVO, 69], Sep. 2021
  (公社)日本獣医学会, Japanese
• ワクモ(Dermanyssus gallinae)由来システインプロテアーゼの抗ワクモワクチン抗原としての評価
  有泉 拓馬; 村田 史郎; 藤澤 宗太郎; 伊勢崎 政美; 佐藤 匠; 大石 英司; 種子野 章; 市居 修; 前川 直也; 岡川 朋弘; 今内 覚; 大橋 和彦, 日本獣医学会学術集会講演要旨集, 164回, [EO, 6], Sep. 2021
  (公社)日本獣医学会, Japanese
• ネコの慢性腎臓病における腎臓組織の酸化ストレスと病態との関連性
  藏原 なつめ; 大和 修; 市居 修; 矢吹 映, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 53], Sep. 2021
  (公社)日本獣医学会, Japanese
• マウス全身免疫状態の違いによる異物応答の修飾
  山川 智大; 市居 修; 難波 貴志; 大谷 祐紀; 中村 鉄平; Abdul Masum Md.; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 8], Sep. 2021
  (公社)日本獣医学会, Japanese
• 慢性腎炎における尿と腎盤上皮の病態連関
  市居 修; 細谷 実里奈; Masum Md.Abdul; 堀野 太郎; 中村 鉄平; 難波 貴志; 大谷 祐紀; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 26], Sep. 2021
  (公社)日本獣医学会, Japanese
• マウス卵管線毛上皮細胞と排卵卵子の相互作用 加齢と免疫異常は卵子輸送速度を変化させる
  細谷 実里奈; 市居 修; 渡邉 敬文; 植田 弘美; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 37], Sep. 2021
  (公社)日本獣医学会, Japanese
• 牛の腟前庭にみられたリンパ陰部輪(Genital Lymphoid Ring found in the vaginal vestibule of cow)
  Chuluunbaatar Tsolmon; 市居 修; Masum Md. Abdul; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 38], Sep. 2021
  (公社)日本獣医学会, English
• ネコの慢性腎臓病における腎臓組織の酸化ストレスと病態との関連性
  藏原 なつめ; 大和 修; 市居 修; 矢吹 映, 日本獣医学会学術集会講演要旨集, 164回, [HSO, 53], Sep. 2021
  (公社)日本獣医学会, Japanese
• ワクモ(Dermanyssus gallinae)由来システインプロテアーゼの抗ワクモワクチン抗原としての評価
  有泉 拓馬; 村田 史郎; 藤澤 宗太郎; 伊勢崎 政美; 佐藤 匠; 大石 英司; 種子野 章; 市居 修; 前川 直也; 岡川 朋弘; 今内 覚; 大橋 和彦, 日本獣医学会学術集会講演要旨集, 164回, [EO, 6], Sep. 2021
  (公社)日本獣医学会, Japanese
• 乳用種廃用牛を利用した移植用結合組織体の作製 経時的構成変化の解析
  片山 由佳; 市居 修; 柳瀬 圭太; 中村 鉄平; 池田 哲平; 難波 貴志; 大谷 祐紀; 昆 泰寛; 西邑 隆徳, 日本獣医学会学術集会講演要旨集, 164回, [AO, 9], Sep. 2021
  (公社)日本獣医学会, Japanese
• ヤギ腎臓における血管系リンパ組織の分類(Clarification of vasculature-associated lymphoid tissue in goat kidney)
  Masum Md. Abdul; 市居 修; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 25], Sep. 2021
  (公社)日本獣医学会, English
• シチメンチョウの気管上皮に発現する多様な鳥インフルエンザウイルスレセプターの構造解析
  小林 大樹; 市居 修; 日尾野 隆大; 西原 祥子; 高瀬 明; 磯田 典和; 迫田 義博, 日本獣医学会学術集会講演要旨集, 164回, [DVO, 69], Sep. 2021
  (公社)日本獣医学会, Japanese
• 骨軟骨組織に対する新規多重染色法(JFRL染色)の有用性
  中村 鉄平; 鷲見 嘉奈子; 辻 絵里香; 都築 奈津子; 近藤 大輔; 辻尾 祐志; 細谷 実里奈; 難波 貴志; 入江 隆夫; 市居 修; 昆 泰寛; 三嶋 隆, 日本獣医学会学術集会講演要旨集, 164回, [AO, 5], Sep. 2021
  (公社)日本獣医学会, Japanese
• Interleukin-36受容体を欠損した自己免疫性腎炎モデルマウスの腎表現型解析
  難波 貴志; 市居 修; 岡村 匡史; 中野 堅太; 中村 鉄平; 大谷 祐紀; Abdul Masum Md.; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 27], Sep. 2021
  (公社)日本獣医学会, Japanese
• Health impact assessment of pet cats (Felis silvestris catus) caused by exposure to organohalogen contaminants by serum metabolomics and thyroid hormone analysis
  野見山桂; 山本恭央; 江口哲史; 西川博之; 水川葉月; 横山望; 市居修; 滝口満喜; 中山翔太; 池中良徳; 石塚真由美; 国末達也, 環境ホルモン学会研究発表会プログラム・要旨集, 23回, 55, 55, Sep. 2021
  環境ホルモン学会(日本内分泌撹乱化学物質学会), Japanese
• 汎動物学からみた身近な動物の腎臓とその病態
  市居 修, 日本腎臓学会誌, 63, 4, 385, 385, Jun. 2021
  (一社)日本腎臓学会, Japanese
• Analysis of variation of serum metabolites in cats by BDE209 long-term exposure test using Smart Metabolite Database
  野見山桂; 林多聞; 江口哲史; 水川葉月; 高口倖暉; 横山望; 市居修; 滝口満喜; 笹岡一慶; 中山翔太; 池中良徳; 石塚真由美; 国末達也, 環境化学討論会要旨集(CD-ROM), 29th, 2021
• Receptor structures that contribute to the propagation of human H1N1 influenza A viruses in embryonated chicken eggs
  高瀬明; 高瀬明; 一宮智美; 岡松正敏; 木下貴明; 木下貴明; 小林大樹; 市居修; 山本直樹; 山本直樹; 迫田義博; 迫田義博; 喜田宏; 川島博人; 山本一夫; 西原祥子; 西原祥子, 日本糖質学会年会要旨集, 40th, 2021
• Human H1N1 influenza A viruses utilize sulfated glycans containing α2,3-linked sialic acid expressed on the membranes of chicken embryos as receptors
  高瀬明; 高瀬明; 一宮智美; 岡松正敏; 木下貴明; 木下貴明; 小林大樹; 市居修; 市居修; 山本直樹; 山本直樹; 迫田義博; 迫田義博; 喜田宏; 川島博人; 山本一夫; 西原祥子; 西原祥子, 日本ウイルス学会学術集会プログラム・予稿集(Web), 68th, 2021
• 新たに発見した尿路関連リンパ組織の役割
  市居修, 医科学応用研究財団研究報告(CD-ROM), 38, 2021
• 『下部消化管の腫瘍外科』解剖学
  市居修, 日本獣医麻酔外科学会オンライン学術集会抄録集(Web), 102nd, 2021
• 汎動物学からみた身近な動物の腎臓とその病態
  市居修, 日本腎臓学会誌(Web), 63, 4, 2021
• コットンラットの上行結腸に独特な形態がみられる(Unique morphology found in the ascending colon of cotton rats)
  Chuluunbaatar Tsolmon; 市居 修; 中村 鉄平; 入江 隆夫; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 178, 178, Oct. 2020, [International Magazine]
  Cotton rats are one of the experimental rodents used for testing different infectious and non-infectious diseases, including gastrointestinal tract pathology. However, their intestinal morphological characteristics are still poorly understood. Here, we clarified the anatomical and histological characteristics of the cecum and ascending colon (AC) of young (1-3-month old), adult (4-6-month old), and old (10-12-month old) cotton rats. The large intestine (LI) in cotton rats is composed of the cecum, AC, transverse and descending colons, and rectum, and is similar to that of other mammals. The AC begins with a double or triple spiral loop-like flexure (SLLF) and ends with a coupled horseshoe-like flexure (HSLF). A single longitudinal mucosal fold (SLMF) was found at the beginning of the AC along the mesentery line and developed with age. Furthermore, the SLMF contained several lymphatic nodules (LNs), indicating their role in digestive and immunological functions. Small and large protuberant LNs were found in the cecum and SLLF, respectively, whereas thin and flat LNs were observed in the HSLF and transverse colon, respectively. Regarding sex-related differences, adult females had a significantly longer AC with a higher number of SLLFs compared to males. The SLMF length and LN number were also longer and higher, respectively, in adult females compared to adult males. These are crucial findings, indicating the presence of sex-related differences in the morphology of the LI in cotton rats, and ours is the first study to discover a sex difference in the mammalian LI lining. Our study clarified the unique morphology of the LI in cotton rats, which could serve as the principal model for elucidating species-specific digestive tract functions and gastrointestinal disorders., (公社)日本獣医学会, English
• 新生仔コットンラット(Sigmodon hispidus)の成長過程における卵巣の変化(Developmental changes of the ovary in the neonatal cotton rat(Sigmodon hispidus))
  Islam Md. Rashedul; 市居 修; 中村 鉄平; 入江 隆夫; Masum Md. Abdul; Yaser Elewa Ali Hosny; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 182, 182, Oct. 2020, [International Magazine]
  The reproductive characteristics and ovarian development in cotton rats (Sigmodon hispidus, CRs) are unclear, although CRs are commonly used as animal models in biomedical research. We previously reported that young (6-8 weeks) CRs showed multi-oocyte follicles (MOFs) and double nucleated oocytes (DNOs) in different stages of follicles. The developmental changes in neonatal CR ovaries were investigated in the present study and were compared with our findings in previous studies of unique phenotypes, particularly in oocytes. CR ovaries at postnatal days (PND) 0, 4, and 7 were obtained from the Hokkaido Institute of Public Health. Samples were analyzed by light and transmission electron microscopy. The general histology and folliculogenesis in CR ovaries were similar to those in other experimental rodents. However, DNOs were observed in all age categories and were frequently observed in primordial follicles, whereas MOFs started to develop from PND4 with greater frequency in primary follicles. Almost all developing follicles expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and forkhead box L2, which are representative markers of oocytes and follicular epithelial cells, respectively. Ki-67 staining demonstrated the proliferative activity of granulosa cells, but not of oocytes, in follicles. Moreover, rapid folliculogenesis of CR due to a small number of apoptotic oocytes was suggested, based on results of the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, confirming the formation of DNOs or MOFs. These findings clarify the development of unique phenotypes of neonatal CR ovaries and support it as a useful model to better understand folliculogenesis and oocytogenesis as well as their abnormalities in humans and other animals., (公社)日本獣医学会, English
• 鳥インフルエンザウイルスの中間宿主としてのウズラおよびシチメンチョウにおけるシアル酸糖鎖分布の解析
  小林 大樹; 佐々木 乙; 日尾野 隆大; 岡松 正敏; 西原 祥子; 高瀬 明; 市居 修; 松野 啓太; 迫田 義博; 北海道大人獣共通感染症リサーチセンター/国際協働ユニット, 日本獣医学会学術集会講演要旨集, 163回, 230, 230, Oct. 2020
  (公社)日本獣医学会, Japanese
• コットンラットの尾の組織学的特徴および自切機序の解析
  中村 鉄平; 細谷 実里奈; 市居 修; 入江 隆夫; 寸田 祐嗣; Elewa Yaser Hosny Ali; 三嶋 隆; 渡邉 敬文; 植田 弘美; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 177, 177, Oct. 2020
  (公社)日本獣医学会, Japanese
• 自己免疫疾患モデルマウスを用いた糖尿病性腎臓病の病態解明アプローチ
  平松 栞; 市居 修; 難波 貴志; 大谷 祐紀; Masum Md. Abdul; 中村 鉄平; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 181, 181, Oct. 2020
  (公社)日本獣医学会, Japanese
• 健常猫の腎臓におけるレクチン組織化学的研究
  野口 あやな; 大和 修; 市居 修; 矢吹 映, 日本獣医学会学術集会講演要旨集, 163回, 284, 284, Oct. 2020
  (公社)日本獣医学会, Japanese
• アンドロゲンは自己免疫性腎炎モデルマウスの糸球体硬化を増悪させる
  大谷 祐紀; 市居 修; Masum Md. Abdul; 難波 貴志; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 181, 181, Oct. 2020
  (公社)日本獣医学会, Japanese
• 閉塞性腎疾患モデルマウスにおける、Toll様受容体8シグナル伝達を介したポドサイト障害(Podocyte injury via Toll-like receptor 8 signaling in experimental obstructive nephropathy in mice)
  Masum Md. Abdul; 市居 修; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 181, 181, Oct. 2020
  (公社)日本獣医学会, English
• マウス卵管の線毛におけるヘッジホッグシグナル伝達経路の発現
  細谷 実里奈; 市居 修; 中村 鉄平; 長谷川 靖洋; Masum Md. Abdul; 渡邉 敬文; 植田 弘美; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 183, 183, Oct. 2020
  (公社)日本獣医学会, Japanese
• 自己免疫疾患モデルマウスの解析からみる腎臓内IL-36群の機能的棲み分け
  難波 貴志; 市居 修; 中村 鉄平; 大谷 祐紀; Masum Md. Abdul; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 180, 180, Oct. 2020
  (公社)日本獣医学会, Japanese
• ネコ尿管の形態と尿管結石症による変化
  市居 修; 小山田 和央; 水川 葉月; 中村 鉄平; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 180, 180, Oct. 2020
  (公社)日本獣医学会, Japanese
• Study of mucosa-associated lymphoid tissues in farm animals (III)-to identify more effective regions for their vaccination-
  市居修; 中村鉄平; 小千田圭吾; 山下祐輔; 池田哲平, 食肉に関する助成研究調査成果報告書, 38, 2020
• 動物iBTA組織のoff-the-shelfバイオマテリアルとしての可能性
  佐藤 康史; 岩井 良輔; 西邑 隆徳; 市居 修; 宮本 伸二; 秋吉 秀保; 中山 泰秀, 人工臓器, 48, 3, 219, 222, 15 Dec. 2019
  一般社団法人 日本人工臓器学会, Japanese
• コットンラット(Sigmodon hispidus)卵巣の独特な形態学的特徴(Unique morphological characteristics in the ovary of cotton rat(Sigmodon hispidus))
  Islam Md Rashedul; 市居 修; 中村 鉄平; 入江 隆夫; Masum Md Abdul; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 341, 341, Aug. 2019
  (公社)日本獣医学会, English
• 自己免疫疾患モデルマウスにみられる涙液産生組織の形態学的変化
  平石 真也; 市居 修; 中村 鉄平; 難波 貴志; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 336, 336, Aug. 2019
  (公社)日本獣医学会, Japanese
• コットンラットの下咽頭梨状窩瘻を構成する細胞の組織化学的特徴
  中村 鉄平; 市居 修; 入江 隆夫; 寸田 祐嗣; 細谷 実里奈; 長崎 健一; Ali Elewa Yaser Hosny; 三嶋 隆; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 337, 337, Aug. 2019
  (公社)日本獣医学会, Japanese
• 自己免疫疾患モデルマウスで変化する腎臓内Interleukin 1 familyの発現バランス
  難波 貴志; 市居 修; 中村 鉄平; Ali Elewa Yaser Hosny; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 338, 338, Aug. 2019
  (公社)日本獣医学会, Japanese
• 動物の尿路関連リンパ組織(UTALT)に発現する分子群
  市居 修; 中村 鉄平; 堀野 太郎; 細谷 実里奈; Ali Elewa Yaser Hosny; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 338, 338, Aug. 2019
  (公社)日本獣医学会, Japanese
• 加齢マウスの尿生殖器における局所リンパ組織の探索
  山下 渚; 市居 修; 大谷 裕紀; Md. Abdul Md. Abdul; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 339, 339, Aug. 2019
  (公社)日本獣医学会, Japanese
• マウス生体内異物反応の経時観察 組織局所の炎症と線維化の関連性
  大江 紗央; 市居 修; 難波 貴志; 大谷 祐紀; Masum Md. Abdul; 中村 鉄平; Ali Elewa Yaser Hosny; 西邑 隆徳; 中山 泰秀; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 340, 340, Aug. 2019
  (公社)日本獣医学会, Japanese
• マウスの卵巣嚢-腹腔連絡路(卵巣嚢孔)
  細谷 実里奈; 市居 修; 中村 鉄平; 難波 貴志; Ali Elewa Yaser Hosny; 渡邉 敬文; 植田 弘美; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 340, 340, Aug. 2019
  (公社)日本獣医学会, Japanese
• 精巣摘出が自己免疫疾患モデルマウスの病態形成へ与える影響
  大谷 祐紀; 市居 修; Ali Elewa Yaser Hosny; 中村 鉄平; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 341, 341, Aug. 2019
  (公社)日本獣医学会, Japanese
• ワクモ由来Adipocyte plasma membrane-associated proteinの抗ワクモワクチン抗原としての評価
  森田 鮎; 竹原 昌生; 村田 史郎; 伊勢崎 政美; 藤澤 宗太郎; 種子野 章; 酒井 英史; 宇野 有紀子; 小川 遼; 市居 修; 前川 直也; 岡川 朋弘; 今内 覚; 大橋 和彦, 日本獣医学会学術集会講演要旨集, 162回, 412, 412, Aug. 2019
  (公社)日本獣医学会, Japanese
• 慢性腎臓病の猫の腎臓におけるperoxisome proliferator activated receptor-γの発現動態
  矢吹 映; 上原 由季; 吉田 千紘; 市居 修; 大和 修, 日本獣医学会学術集会講演要旨集, 162回, 469, 469, Aug. 2019
  (公社)日本獣医学会, Japanese
• 詳細な病理学的解析に基づいて治療方針を決定した免疫複合体性糸球体腎炎の犬の1例
  新井田 篤; 大田 寛; 笹岡 一慶; 細谷 謙次; 市居 修; 賀川 由美子; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 滝口 満喜, 北海道獣医師会雑誌, 63, 8, 336, 336, Aug. 2019
  (公社)北海道獣医師会, Japanese
• 新生子MRL/MpJマウスにおいて卵巣マスト細胞は卵巣-卵巣采結合部の方向に遊走する : 局在変化が明らかにするマスト細胞の新たな役割
  ナカムラ テッペイ; イチイ オサム; コン ヤスヒロ, 臨床免疫・アレルギー科 = Clinical immunology & allergology / 臨床免疫・アレルギー科編集委員会 編, 71, 6, 603, 607, Jun. 2019
  科学評論社, Japanese
• 生体内組織形成術を用いて作製したウシ由来バイオシートの性状に及ぼす生体内環境の影響
  今山 知佳; 寺澤 武; 中山 泰秀; 三谷 朋弘; 市居 修; 池田 哲平; 小千田 圭吾; 小林 謙; 鈴木 貴弘; 西邑 隆徳, 日本畜産学会大会講演要旨集, 125回, 172, 172, Mar. 2019
  (公社)日本畜産学会, Japanese
• 組織工学血管修復材(バイオシート)の開発 低圧系から高圧系、異種移植の可能性
  中山 泰秀; 古越 真耶; 今山 知佳; 市居 修; 西邑 隆徳, 日本心臓血管外科学会学術総会抄録集, 49回, [PP, 236], Feb. 2019
  (NPO)日本心臓血管外科学会, Japanese
• 新たに発見した尿路関連リンパ組織の役割
  市居 修, 医科学応用研究財団研究報告 / 鈴木謙三記念医科学応用研究財団 [編], 38, 258, 263, 2019
  鈴木謙三記念医科学応用研究財団, Japanese
• Study of mucosa-associated lymphoid tissues in farm animals (II)-to identify more effective regions for their vaccination-
  市居修; 中村鉄平; 小千田圭吾; 山下祐輔; 池田哲平, 食肉に関する助成研究調査成果報告書, 37, 2019
• A型インフルエンザウイルスに対する交差感染防御免疫への非中和IgA抗体の役割
  奥谷公亮; 吉田玲子; マンズール ラシッド; 齊藤慎二; 鈴木忠樹; 市居修; 東秀明; 高田礼人, 日本ウイルス学会学術集会プログラム・予稿集(Web), 67th, 2019
• コットンラットの下咽頭梨状窩瘻を構成する細胞の組織化学的特徴
  中村鉄平; 中村鉄平; 市居修; 入江隆夫; 寸田祐嗣; 細谷実里奈; 長崎健一; YASER HOSNY; Ali Elewa; 三嶋隆; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 337, 337, 2019
  (公社)日本獣医学会, Japanese
• PCBs曝露によるネコ肝トランスクリプトームへの影響
  水川葉月; NGYUEN Hoa Thanh; 岩田久人; 野見山桂; 池中良徳; 中山翔太; 横山望; 市居修; 滝口満喜; KHIDKHAN Kraisiri; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 28th, ROMBUNNO.1C‐08, 2019
  Japanese
• デカブロモジフェニルエーテル(BDE209)の長期曝露がイエネコへ及ぼす影響
  田中啓介; 野見山桂; 水川葉月; 水川葉月; 高口倖暉; 田上瑠美; 横山望; 市居修; 滝口満喜; 笹岡一慶; 中山翔太; 池中良徳; 石塚真由美; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 28th, ROMBUNNO.1C‐09, 2019
  Japanese
• iBTA心血管系修復再生材の開発 肺動脈、動脈パッチ移植の検討
  中山 泰秀; 古越 真耶; 今山 知佳; 市居 修; 西邑 隆徳, 日本獣医麻酔外科学雑誌, 49, Suppl.2, 252, 252, Dec. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• iBTA僧帽弁の開発 リング付弁の検討
  中山 泰秀; 湯浅 啓史; 市居 修; 西邑 隆徳; 田地川 勉, 日本獣医麻酔外科学雑誌, 49, Suppl.2, 252, 252, Dec. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• Tissue engineeringによる人工臓器作成 iBTA組織のoff-the-shelf人工臓器をめざして ウシバイオシートの異種移植
  中山 泰秀; 大島 奈公子; 巽 英介; 市居 修; 西邑 隆徳, 人工臓器, 47, 2, S, 17, Oct. 2018
  (一社)日本人工臓器学会, Japanese
• “Lymphoid tissues appearing in the urinary tract of animals (Urinary tract-associated lymphoid tissue, UTALT)”.
  O. Ichii; T. Nakamura; R. Ono; T. Horino; Y. H. A. Elewa; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, Sep. 2018
  Japanese
• “Morphological characteristics of the dromedary thyroid gland - and distribution of C cells”.
  T. Nakamura; O. Ichii; Y. H. A. Elewa; W. A. M. Ghonimi; K. Nagasaki; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, Sep. 2018
  Japanese
• “New findings of kidney disease - Kidney lesions found in Casp3 deficient mice”.
  T. Suzuki; O. Ichii; T. Nakamura; Y. H. A. Elewa; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, Sep. 2018
  Japanese
• “Morphological and functional changes observed in bone of autoimmune disease model”.
  T. Namba; O. Ichii; Y. H. A. Elewa; T. Nakamura; J. Kimura; Y. Otani; Md. A. Masum; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, Sep. 2018
  Japanese
• Effect of systemic autoimmune abnormality in MRL/MpJ-Faslpr/lpr mice on the ciliary function.
  M. Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan, 161th, Sep. 2018
  Japanese
• “Mediastinal fat-associated lymphoid cluster and lung asthma development in papain induced lung asthma mice model
  Y. H. A. Elewa; T. Nakamura; O. Ichii; Y. Kon, The 64th annual meeting of the Japanese the Anatomical Society (Oral Presentation). Aomori, Japan., 64th, Sep. 2018
  English
• MRL/MpJ‐Fas^lpr/lprマウスの全身自己免疫異常が線毛運動機能に与える影響
  細谷実里奈; 市居修; ALI ELEWA; Yaser Hosny; 中村鉄平; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 161回, 296, 296, Aug. 2018
  (公社)日本獣医学会, Japanese
• 新たな腎病態マーカー分子の発見と臨床獣医学への応用に関する研究
  市居修, 日本獣医学会学術集会講演要旨集, 161回, 161, 161, Aug. 2018
  (公社)日本獣医学会, Japanese
• 動物の尿路に出現するリンパ組織(Urinary tractassociated lymphoid tissue,UTALT)
  市居修; 中村鉄平; 中村鉄平; 細谷実里奈; 堀野太郎; ALI ELEWA Yaser Hosny; 昆泰寛, 日本獣医学会学術集会講演要旨集, 161回, 294, 294, Aug. 2018
  (公社)日本獣医学会, Japanese
• 自己免疫疾患モデルマウスの骨に観察される形態機能的変化
  難波貴志; 市居修; 中村鉄平; 木村純平; 木村純平; 大谷祐紀; ABDUL Masum Md; HOSNY Ali Elewa Yaser; 昆泰寛, 日本獣医学会学術集会講演要旨集, 161回, 285, 285, Aug. 2018
  (公社)日本獣医学会, Japanese
• 腎疾患の新知見―Casp3欠損マウスにみられる腎病変―
  鈴木崇史; 市居修; 中村鉄平; 中村鉄平; ALI ELEWA Yaser Hosny; 昆泰寛, 日本獣医学会学術集会講演要旨集, 161回, 294, 294, Aug. 2018
  (公社)日本獣医学会, Japanese
• 自己免疫疾患モデルマウスで増加する減数分裂特異的精母細胞アポトーシスの原因探索
  大谷祐紀; 市居修; ALI ELEWA; Yaser Hosny; 木村純平; MASUM Md. Abdul; 中村鉄平; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 161回, 295, 295, Aug. 2018
  (公社)日本獣医学会, Japanese
• ウシの眼関連リンパ組織について
  小千田圭吾; 市居修; 中村鉄平; 中村鉄平; 池田哲平; 山下祐輔; 長澤裕哉; ALI ELEWA Yaser Hosny; 昆泰寛, 日本獣医学会学術集会講演要旨集, 161回, 288, 288, Aug. 2018
  (公社)日本獣医学会, Japanese
• ヒトコブラクダ甲状腺の形態学的特徴―鰓後体遺残とC細胞の分布―
  中村鉄平; 中村鉄平; 市居修; ALI ELEWA; Yaser Hosny; ALI ELEWA Yaser Hosny; 細谷実里奈; GHONIMI Wael A. M; 辰巳治; 長崎健一; 昆泰寛, 日本獣医学会学術集会講演要旨集, 161回, 292, 292, Aug. 2018
  (公社)日本獣医学会, Japanese
• マウス自己免疫性糸球体炎において見出された有足細胞傷害はToll様受容体9の過剰発現と関連する(Overexpression of Toll-like receptor 9 correlates with podocyte injury found in murine autoimmune glomerulonephritis)
  Masum Md. Abdul; 市居 修; Elewa Yaser Hosny Ali; 中村 鉄平; 大谷 祐紀; 細谷 実里奈; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 161回, 294, 294, Aug. 2018
  (公社)日本獣医学会, English
• “Glomerular lesions seen in male cotton rats (Sigmodon hispidus)”
  T. Nakamura; O. Ichii; T. Irie; H. Kouguchi; Sunden, Masum; M. A., Y; Y. H. A. Elewa; Y. Kon, The 11th Annual Meeting of the Japanese Society of Veterinary Renal Urology., 11th, Aug. 2018
  Japanese
• 異種バイオシートの血管修復材への応用 肺動脈パッチモデルによる検討
  古越 真耶; 今山 知佳; 市居 修; 西邑 隆徳; 中山 泰秀, 日本獣医麻酔外科学雑誌, 49, Suppl.1, 272, 272, Jun. 2018
  (一社)日本獣医麻酔外科学会, Japanese
• ワクモ(Dermanyssus gallinae)由来カテプシンL用タンパク質の性状解析とワクチン抗原としての評価
  村田 史郎; 伊勢崎 政美; 谷口 綾香; 北條 巧; 種子野 章; 酒井 英史; 宇野 有紀子; 矢吹 卓也; 市居 修; 伊東 拓也; 今内 覚; 大橋 和彦, 衛生動物, 69, 2, 103, 103, Jun. 2018
  日本衛生動物学会, Japanese
• イヌ肺動脈に異種移植したウシ由来バイオシートの組織構造
  今山 知佳; 鈴木 貴弘; 小林 謙; 三谷 朋弘; 市居 修; 中山 泰秀; 古越 真耶; 池田 哲平; 小千田 圭吾; 西邑 隆徳, 日本畜産学会大会講演要旨集, 124回, 115, 115, Mar. 2018
  (公社)日本畜産学会, Japanese
• Local CD34-positive capillary decreased with the progression of lesion in respective area of kidney in mice.
  Md. A. Masum; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 17th International Conference on Nephrology and Urology. London, UK. 13th March 2018., 17th, Mar. 2018
  English
• 肺動脈修復材としての異種結合組織膜バイオシートの開発
  古越真耶; 中山泰秀; 巽英介; 西邑隆徳; 市居修, 日本心臓血管外科学会学術総会(Web), 48th, ROMBUNNO.OP23‐1 (WEB ONLY), 961, Feb. 2018
  (NPO)日本心臓血管外科学会, Japanese
• 肺動脈修復材としての異種結合組織膜バイオシートの開発
  古越 真耶; 中山 泰秀; 巽 英介; 西邑 隆徳; 市居 修, 日本心臓血管外科学会学術総会抄録集, 48回, 960, 961, Feb. 2018
  (NPO)日本心臓血管外科学会, Japanese
• イヌ肺動脈に異種移植したウシ由来バイオシートの組織構造
  今山知佳; 鈴木貴弘; 小林謙; 三谷朋弘; 市居修; 中山泰秀; 古越真耶; 池田哲平; 小千田圭吾; 西邑隆徳, 日本畜産学会大会講演要旨, 124th, 115, 115, 2018
  (公社)日本畜産学会, Japanese
• 有機ハロゲン化合物曝露によるネコの生化学マーカーの変化および甲状腺機能に及ぼす影響評価
  水川葉月; KHIDKHAN Kraisiri; 高口倖暉; 横山望; 市居修; 滝口満喜; 笹岡一慶; 池中良徳; 池中良徳; 中山翔太; TSEND‐AYUSH Sainnoxoi; 野見山桂; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 27th, ROMBUNNO.2A‐10, 2018
  Japanese
• ネコのin vivo BDE209長期曝露試験による体内動態の解明および甲状腺ホルモンへの影響評価
  田中啓介; 野見山桂; 水川葉月; 高口倖暉; 田上瑠美; 横山望; 市居修; 滝口満喜; 笹岡一慶; 中山翔太; 池中良徳; 石塚真由美; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 27th, ROMBUNNO.1A‐03(PA‐13), 2018
  Japanese
• 異種バイオシートの血管修復材への応用:肺動脈パッチモデルによる検討
  古越真耶; 古越真耶; 今山知佳; 市居修; 西邑隆徳; 中山泰秀; 中山泰秀, 日本獣医麻酔外科学雑誌, 49, Supplement 1, 272, 272, 2018
  (一社)日本獣医麻酔外科学会, Japanese
• ワクモ(Dermanyssus gallinae)由来カテプシンL用タンパク質の性状解析とワクチン抗原としての評価
  村田史郎; 伊勢崎政美; 谷口綾香; 北條巧; 種子野章; 酒井英史; 宇野有紀子; 矢吹卓也; 市居修; 伊東拓也; 今内覚; 大橋和彦, 衛生動物, 69, 2, 103, 103, 2018
  日本衛生動物学会, Japanese
• Effects of PCB-exposure on thyroid hormone homeostasis in dog (canis lupus familiaris) and cat (felis catus)
  TAKAGUCHI Kohki; NOMIYAMA Kei; NISHIKAWA Hiroyuki; MIZUKAWA Hazuki; TANOUE Rumi; KUSAKI Momoko; YOKOYAMA Nozomu; ICHII Osamu; TAKIGUCHI Mitsuyoshi; NAKAYAMA Shouta; IKENAKA Yoshinori; ISHIZUKA Mayumi; IWATA Hisato; KUNISUE Tatsuya; TANABE Shinsuke, Annual Meeting of the Japanese Society of Toxicology, 45.1, Suppl., P-72, S245, Jun. 2018
  

  [in Japanese]

  , The Japanese Society of Toxicology, Japanese
• Study of mucosa-associated lymphoid tissues in farm animals-to identify more effective regions for their vaccination-
  市居修; 中村鉄平; 小千田圭吾; 山下祐輔; 池田哲平, 食肉に関する助成研究調査成果報告書, 36, 2018
• 自家移植から異種移植までバイオシートの腹壁欠損再生材への応用
  大島奈公子; 古越真耶; 今山知佳; 西邑隆徳; 市居修; 巽英介; 中山泰秀, 日本再生医療学会総会(Web), 17th, 2018
• 異種バイオシートの血管再生材への応用:ウシバイオシートのビーグル犬肺動脈移植評価
  古越真耶; 古越真耶; 今山知佳; 西邑隆徳; 市居修; 巽英介; 中山泰秀; 中山泰秀, 日本再生医療学会総会(Web), 17th, 2018
• 黄色ブドウ球菌性乳房炎における乳汁中乳腺上皮細胞の割合は乳腺組織の損傷を反映する
  長澤 裕哉; 菊 佳男; 菅原 和恵; 田邉 扶由子; 石川 義春; 市居 修; 野地 智法; 麻生 久; 昆 泰寛; 門田 耕一; 林 智人, 家畜感染症学会誌, 6, 4, 165, 165, Dec. 2017
  家畜感染症学会誌編集委員会, Japanese
• ポリ塩化ビフェニル(PCBs)がネコの甲状腺機能に及ぼす影響
  水川 葉月; 高居 名菜; 野見山 桂; 高口 倖暉; 横山 望; 市居 修; 滝口 満喜; 池中 良徳; 中山 翔太; 石塚 真由美, 環境ホルモン学会研究発表会要旨集, 20回, 78, 78, Dec. 2017
  環境ホルモン学会(日本内分泌撹乱化学物質学会), Japanese
• BXSB/MpJ-Yaa MICE SHOW THE INCREASE OF APOPTOTIC SPERMATOCYTES IN STAGE Ⅻ SEMINIFEROUS TUBULES.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA). Kuching, Sarawak, Malaysia. October 12-13, 2017., 6th, Oct. 2017
  English
• ABNOLMALITY OF OVULATION AND OOCYTE-PICK-UP IN MRL/MpJ-Faslpr/lpr MICE.
  Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; S. Otsuka-Kanazawa; Y. Kon, ABNOLMALITY OF OVULATION AND OOCYTE-PICK-UP IN MRL/MpJ-Faslpr/lpr MICE. The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia, 6th, Oct. 2017
  English
• IL-1F6/IL-36α SIGNALING REGULATES RENAL INFLAMMATION.
  O. Ichii; J. Kimura; T. Okamura; T. Horino; T. Nakamura; H. Sasaki; H. A. Elewa; Y. Kon, The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia, 6th, Oct. 2017
  English
• FAT ACCUMULATION CORRELATES WITH DIABETES MELLITUS IN MALE COTTON RATS (SIGMODON HISPIDUS).
  T. Nakamura; O. Ichii; T. Irie; H. Kouguchi; Y. Sunden; K.-I. Nagasaki; Y. H. A. Elewa; Y. Kon, The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia, Oct. 2017
• “Mechanism and abnormality of ovulation and picking up of mammalian oocytes-Immune factors affecting the molecular morphology of the oviduct.”
  M. Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 5th Sapporo Summer Seminar for One Health (SASSOH). September 20-21, 2017. Sapporo, Japan., 5th, Sep. 2017
  English
• “Loss of endothelial fenestration correlates with podocyte injury in the mouse model of membranoproliferative glomerulonephritis.”
  Md. A. Masum; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 5th Sapporo Summer Seminar for One Health (SASSOH). September 20-21, 2017. Sapporo, Japan., 5th, Sep. 2017
  English
• “The unique phenotype in ovulation and corpus luteum formation in MRL/MpJ mouse.”
  M. Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 160th Meeting of the Japanese Society of Veterinary Sciences, September 13-16, 2017. Kagoshima, Japan., 160th, Sep. 2017
  Japanese
• Histopathological features observed in the testis of autoimmune-prone BXSB/MpJ-Yaa mice.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 5th Sapporo Summer Seminar for One Health., 5th, Sep. 2017
  English
• Apoptosis of spermatocytes seen in BXSB / MpJ-Yaa mice - association with autoimmune abnormality.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 160th Meeting of the Japanese Society of Veterinary Sciences., 160th, Sep. 2017
  Japanese
• “Dual effects of bleomycin on the intra-thoracic immune hemostasis and lung injury in autoimmune disease model mice”.
  Y. H. A. Elewa; T. Nakamura; O. Ichii; Y. Kon, The 63th annual meeting of the Japanese the Anatomical Society., 63th, Sep. 2017
  English
• マウス糸球体の自己免疫関連傷害における内皮細胞と有足細胞との病理学的相関性(Pathological correlations between endothelial cells and podocytes in autoimmune-related injury of the mouse glomerulus)
  Masum Md. Abdul; 市居 修; Elewa Yaser Hosny Ali; 中村 鉄平; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 160回, 310, 310, Aug. 2017
  (公社)日本獣医学会, English
• Elucidation of mammalian reproductive - autoimmune coupling mechanism and analysis of pathological conditions caused by its failure.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura, Y. Kon. The 35th Scientific annual joint meeting of Hokkaido Veterinary Anatomy, 35th, Aug. 2017
  Japanese
• Caspase3欠損マウスからみる細胞死―自己免疫連関異常の新たなエビデンス
  市居修; 堀野太郎; 大田寛; 鈴木崇史; 中村鉄平; 中村鉄平; ELEWA Yaser; Hosny Ali; 昆泰寛, 日本獣医学会学術集会講演要旨集, 160回, 310, 310, Aug. 2017
  (公社)日本獣医学会, Japanese
• Sarcocystis属原虫の終宿主候補としてのキタキツネにおける調査
  入江隆夫; 浦口宏二; 中村鉄平; 中村鉄平; 市居修; 高井伸二; 八木欣平, 日本獣医学会学術集会講演要旨集, 160回, 335, 335, Aug. 2017
  (公社)日本獣医学会, Japanese
• 犬と猫の慢性腎臓病における腎臓へのマクロファージ浸潤
  大原優美子; 矢吹映; 市居修; 水川葉月; 横山望; 中村麗菜, 日本獣医学会学術集会講演要旨集, 160回, 473, 473, Aug. 2017
  (公社)日本獣医学会, Japanese
• BXSB/MpJ‐Yaaマウスにみられる精母細胞のアポトーシス―自己免疫異常との関連性―
  大谷祐紀; 市居修; ELEWA Yaser; Hosny Ali; 木村純平; MASUM Md. Abdul; 中村鉄平; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 160回, 312, 312, Aug. 2017
  (公社)日本獣医学会, Japanese
• 雌性コットンラットの胸腺にみられる加齢性変化
  中村鉄平; 中村鉄平; 市居修; 入江隆夫; 篠原明雄; 孝口裕一; 越本知大; 長崎健一; 寸田祐嗣; ELEWA Yaser Hosny; 辰巳治; 昆泰寛, 日本獣医学会学術集会講演要旨集, 160回, 309, 309, Aug. 2017
  (公社)日本獣医学会, Japanese
• MRL/MpJマウスの排卵―黄体形成過程にみられる特異な表現型
  細谷実里奈; 市居修; ELEWA Yaser; Hosny Ali; 中村鉄平; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 160回, 313, 313, Aug. 2017
  (公社)日本獣医学会, Japanese
• ウシ生体内で形成させた移植医療用組織の構造
  今山知佳; 鈴木貴弘; 小林謙; 三谷朋弘; 市居修; 小千田圭吾; 池田哲平; 寺澤武; 中山泰秀; 西邑隆徳, 日本畜産学会大会講演要旨, 122回, 175, 175, Mar. 2017
  (公社)日本畜産学会, Japanese
• 家畜生体内で再生医療移植用組織をつくる
  西邑隆徳; 今山知佳; 鈴木貴弘; 小林謙; 三谷朋弘; 市居修; 小千田圭吾; 池田哲平; 寺澤武; 中山泰秀, 日本畜産学会大会講演要旨, 122回, 175, 175, Mar. 2017
  (公社)日本畜産学会, Japanese
• MicroRNA研究の実際と問題点 腎臓病を知らせるmicroRNA 病態マーカーとしての可能性と問題点
  市居 修, 日本毒性病理学会講演要旨集, 33回, 52, 52, Jan. 2017
  日本毒性病理学会, Japanese
• ポリ塩化ビフェニル(PCBs)暴露によるネコ甲状腺機能への影響評価~甲状腺ホルモンの脱ヨード化と代謝能の解析~
  水川葉月; 高居名菜; 横山望; 市居修; 滝口満喜; 野見山桂; 高口倖暉; 西川博之; 池中良徳; 池中良徳; 中山翔太; SAINNOXOI Tsend‐ayush; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐01, 2017
  Japanese
• PCBs曝露がイヌ・ネコの甲状腺ホルモン恒常性へ及ぼす影響
  高口倖暉; 野見山桂; 西川博之; 水川葉月; 田上瑠美; 芳之内結加; 横山望; 市居修; 滝口満喜; 中山翔太; 池中良徳; 石塚真由美; 岩田久人; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐02, 2017
  Japanese
• イエネコのin vivo長期曝露試験:デカブロモジフェニルエーテル(BDE209)は甲状腺機能亢進症を引き起こすのか?
  水川葉月; 野見山桂; 池中良徳; 池中良徳; 中山翔太; SAENGTIENCHAI Aksorn; SAINNOXOI Tsendayush; 横山望; 笹岡一慶; 市居修; KHIDKHAN Kraisiri; 高居名菜; 滝口満喜; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐03, 2017
  Japanese
• Thyroid hormone effects of polychlorinated biphenyls (PCBs) in cats-Analysis of deiodination and metabolism-
  MIZUKAWA Hazuki; TAKAI Nana; YOKOYAMA Nozomu; ICHII Osamu; TAKIGUCHI Mitsuyoshi; NOMIYAMA Kei; TAKAGUCHI Kohki; NISHIKAWA Hiroyuki; IKENAKA Yoshinori; NAKAYAMA Shouta M.M.; SAINNOXOI Tsend-ayush; TANABE Shinsuke; ISHIZUKA Mayumi, Annual Meeting of the Japanese Society of Toxicology, 44.1, Suppl., P-55, S236, Jun. 2017
  有機ハロゲン化合物の一種であるポリ塩化ビフェニル（PCBs）は甲状腺ホルモン（THs）の恒常性かく乱が懸念されており、実際、ヒトやげっ歯類においてはPCBs暴露により血中THs濃度の低下が認められている。ヒトの身近な動物であるネコは餌などを介してPCBsに暴露していると懸念され、THs恒常性に何らかの悪影響をもたらすことが予想される。本研究では、ネコの甲状腺機能に対するPCBsの影響を解析するため、<i>in vivo</i>投与試験したネコを用いて甲状腺機能への影響を解析した。血中のTHs濃度（総T4、総T3、遊離T4、遊離T3、リバースT3）を解析した結果、対照群と暴露群の間に有意な差は見られなかった。また、THsの恒常性に関与する遺伝子（甲状腺ホルモン受容体αおよびβ、脱ヨード酵素type1）の肝臓中発現量やTHs代謝に関わる酵素であるグルクロン酸抱合酵素（UGT1A1）の活性を調べたところ、対照群と暴露群の間に有意な差は見られなかった。<br>本実験結果では、ネコにおいてPCBs短期暴露によるTHsへの影響が認められなかったが、日本のペットネコにおけるモニタリング調査では、血中のTHs濃度とPCBs濃度との間に負の相関が見られており、有機ハロゲン化合物によるTHsかく乱が懸念されている。また、本研究試料のメタボローム解析結果では酸化ストレスの発現、抗酸化物質、電子伝達物質の生産阻害などが示唆されており、PCBsや水酸化代謝物による内分泌撹乱作用が心配される。THsの恒常性は非常に複雑な機構のもと維持されていることが知られており、負のフィードバック機構に加え、THsの活性化や代謝、排泄の調整にも多くの遺伝子やトランスポーター、酵素など様々な要因が関わっていることから、ネコは長期的かつ慢性的に化学物質に暴露されることで、甲状腺機能などへの悪影響が現れると考えられ、内分泌撹乱作用の作用機序について解析を進めることが重要である。, The Japanese Society of Toxicology, Japanese
• ネコの甲状腺機能に対するポリ塩化ビフェニル(PCBs)の影響評価~甲状腺ホルモンの脱ヨード化と代謝能の解析~
  水川葉月; 高居名菜; 横山望; 市居修; 滝口満喜; 野見山桂; 高口倖暉; 西川博之; 池中良徳; 池中良徳; 中山翔太; SAINNOXOI Tsend‐Ayush; 田辺信介; 石塚真由美, Journal of Toxicological Sciences, 42, Supplement, S236, S236, 2017
  有機ハロゲン化合物の一種であるポリ塩化ビフェニル（PCBs）は甲状腺ホルモン（THs）の恒常性かく乱が懸念されており、実際、ヒトやげっ歯類においてはPCBs暴露により血中THs濃度の低下が認められている。ヒトの身近な動物であるネコは餌などを介してPCBsに暴露していると懸念され、THs恒常性に何らかの悪影響をもたらすことが予想される。本研究では、ネコの甲状腺機能に対するPCBsの影響を解析するため、in vivo投与試験したネコを用いて甲状腺機能への影響を解析した。血中のTHs濃度（総T4、総T3、遊離T4、遊離T3、リバースT3）を解析した結果、対照群と暴露群の間に有意な差は見られなかった。また、THsの恒常性に関与する遺伝子（甲状腺ホルモン受容体αおよびβ、脱ヨード酵素type1）の肝臓中発現量やTHs代謝に関わる酵素であるグルクロン酸抱合酵素（UGT1A1）の活性を調べたところ、対照群と暴露群の間に有意な差は見られなかった。
  本実験結果では、ネコにおいてPCBs短期暴露によるTHsへの影響が認められなかったが、日本のペットネコにおけるモニタリング調査では、血中のTHs濃度とPCBs濃度との間に負の相関が見られており、有機ハロゲン化合物によるTHsかく乱が懸念されている。また、本研究試料のメタボローム解析結果では酸化ストレスの発現、抗酸化物質、電子伝達物質の生産阻害などが示唆されており、PCBsや水酸化代謝物による内分泌撹乱作用が心配される。THsの恒常性は非常に複雑な機構のもと維持されていることが知られており、負のフィードバック機構に加え、THsの活性化や代謝、排泄の調整にも多くの遺伝子やトランスポーター、酵素など様々な要因が関わっていることから、ネコは長期的かつ慢性的に化学物質に暴露されることで、甲状腺機能などへの悪影響が現れると考えられ、内分泌撹乱作用の作用機序について解析を進めることが重要である。, The Japanese Society of Toxicology, Japanese
• 血清中メタボローム解析によるイエネコのPCBs毒性影響評価
  野見山桂; 西川博之; 水川葉月; 高口倖暉; SAINNOXOI Tsend‐ayush; 横山望; 市居修; 滝口満喜; 石塚真由美; 池中良徳; 中山翔太; 江口哲史; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐05, 2017
  Japanese
• イエネコのin vivo長期曝露試験:デカブロモジフェニルエーテル(BDE209)の生体内変化
  田中啓介; 野見山桂; 水川葉月; 高口倖暉; 田上瑠美; SAENGTIENCHAI Aksorn; SAINNOXOI Tsend‐ayush; 横山望; 市居修; 滝口満喜; 中山翔太; 池中良徳; 石塚真由美; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 26th, ROMBUNNO.2A‐04, 2017
  Japanese
• 鳥類医学の発展に向けた新規腎障害マーカーの探索
  石井千尋; 池中良徳; 川合佑典; 市居修; 中山翔太; 水川葉月; 石塚真由美, 日本鳥学会大会講演要旨集, 2017, 100, 2017
  Japanese
• ポリ塩化ビフェニル(PCBs)がネコの甲状腺機能に及ぼす影響
  水川葉月; 高居名菜; 野見山桂; 高口倖暉; 横山望; 市居修; 滝口満喜; 池中良徳; 池中良徳; 中山翔太; 石塚真由美, 日本内分泌かく乱化学物質学会研究発表会要旨集, 20th, 78, 78, 2017
  環境ホルモン学会(日本内分泌撹乱化学物質学会), Japanese
• 腎臓病を知らせるmicroRNA―病態マーカーとしての可能性と問題点―
  市居修, 日本毒性病理学会講演要旨集, 33rd, 52 (JA),154 (EN), 52, 2017
  (一社)日本毒性病理学会, Japanese
• 生体内組織形成術による組織厚制御の可能性:管状組織体の作製
  寺澤武; 寺澤武; 西邑隆徳; 三谷朋弘; 市居修; 中山泰秀; 中山泰秀, 再生医療, 16, 336, 2017
  Japanese
• 黄色ブドウ球菌性乳房炎における乳汁中乳腺上皮細胞の割合は乳腺組織の損傷を反映する
  長澤 裕哉; 菊 佳男; 菅原 和恵; 田邉 扶由子; 石川 義春; 市居 修; 野地 智法; 麻生 久; 昆 泰寛; 門田 耕一; 林 智人, 家畜感染症学会誌, 5, 4, 180, 180, Dec. 2016
  家畜感染症学会誌編集委員会, Japanese
• 生体内組織形成術による管状組織体の壁厚制御
  寺澤 武; 西邑 隆徳; 三谷 朋弘; 市居 修; 中山 泰秀, 人工臓器, 45, 2, S, 129, Oct. 2016
  (一社)日本人工臓器学会, Japanese
• X線照射はミトコンドリア生合成を亢進することなく細胞内ミトコンドリア含量を増大させる
  山盛徹; 笹川朋哉; 市居修; 房知輝; 昆泰寛; 稲波修, 日本放射線影響学会大会抄録(Web), 59回, 13, 13, Oct. 2016
  (一社)日本放射線影響学会, Japanese
• マウスにおける縦隔脂肪関連リンパクラスターとブレオマイシン誘発性肺炎との病理組織学的関連(Histopathological correlations between the mediastinal fat associated lymphoid clusters and bleomycin-induced pneumonitis in mice)
  Ali Elewa Yaser Hosny; Masum Md.Abdul; 中村 鉄平; 市居 修; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 159回, 302, 302, Aug. 2016
  (公社)日本獣医学会, English
• マウス腎臓病モデルにおける疾患進行に伴うCD34陽性毛細血管の減少(CD34-positive capillary decreased with the progression of kidney disease in mouse models)
  Masum Md.Abdul; Yaser Hosny Ali Elewa; 中村 鉄平; 市居 修; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 159回, 308, 308, Aug. 2016
  (公社)日本獣医学会, English
• “Histopathological evaluation of the possible protective role of royal jelly and cod liver oil against reproductive toxicity of tartrazine in adult male albino rats.”
  Y. H. A. Elewa; A. Moustafa; A.A. Mohamed; A. A.A. Galal; O. Ichii; Y. Kon, The 8th international Toxicology symposium in Africa. Cairo, Egypt., 8th, Aug. 2016
  English
• エゾシカに寄生するSarcocystis属原虫の終宿主動物探索
  入江隆夫; 池田徹也; 中村鉄平; 中村鉄平; 市居修; 山田智子; 伊東拓也; 山崎朗子; 高井伸二; 八木欣平, 日本獣医学会学術集会講演要旨集, 159回, 338, 338, Aug. 2016
  (公社)日本獣医学会, Japanese
• ウシ咽頭扁桃は鼻腔投与した黄色ブドウ球菌死菌に対する免疫応答の場になる
  長澤裕哉; 菊佳男; 菅原和恵; 田邉扶由子; 石川義春; 野地智法; 市居修; 昆泰寛; 麻生久; 門田耕一; 林智人, 日本獣医学会学術集会講演要旨集, 159回, 362, 362, Aug. 2016
  (公社)日本獣医学会, Japanese
• 芳香族炭化水素受容体シグナルの活性化は胃粘膜異常を増悪する―遺伝子改変モデルマウスを用いたin vivo解析―
  團塚愛; HANBERG Annika; 中村鉄平; 中村鉄平; 市居修; 昆泰寛, 日本獣医学会学術集会講演要旨集, 159回, 314, 314, Aug. 2016
  (公社)日本獣医学会, Japanese
• コットンラットにおけるインスリン抵抗性と脂肪蓄積との関連
  中村鉄平; 中村鉄平; 市居修; 入江隆夫; 寸田祐嗣; 孝口裕一; 長崎健一; 吉安友二; 昆泰寛, 日本獣医学会学術集会講演要旨集, 159回, 304, 304, Aug. 2016
  (公社)日本獣医学会, Japanese
• 自己免疫疾患モデルMRL/MpJ-Faslprマウスにみられる卵子のキャッチアップ障害
  細谷 実里奈; 中村 鉄平; Hosny Ali Elewa Yaser; 市居 修; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 159回, 314, 314, Aug. 2016
  (公社)日本獣医学会, Japanese
• 鶏胸大動脈におけるグロムス細胞からの5-HT放出に対するH2Sの作用(Effect of H2S on 5-HT release from glomus cells in the chicken thoracic aorta)
  Delgermurun Dugar; 山口 聡一郎; 市居 修; 昆 泰寛; 伊藤 茂男; 乙黒 兼一, 日本獣医学会学術集会講演要旨集, 159回, 495, 495, Aug. 2016
  (公社)日本獣医学会, English
• マウス遠位尿細管の嚢胞形成に関与する新規原因遺伝子座の役割
  市居修; 中村鉄平; 中村鉄平; 矢吹映; 堀野太郎; 昆泰寛, 日本獣医学会学術集会講演要旨集, 159回, 308, 308, Aug. 2016
  (公社)日本獣医学会, Japanese
• マウス胎子腸管器官培養法により形成された十二指腸空腸曲の解析
  尾之内佐和; 市居修; 昆泰寛, 日本獣医学会学術集会講演要旨集, 159回, 302, 302, Aug. 2016
  (公社)日本獣医学会, Japanese
• 抗ワクモ(Dermanyssus gallinae)ワクチン開発に向けたワクモ由来フェリチン2の性状解析
  伊勢崎政美; 村田史郎; 酒井英史; 矢吹卓也; 種子野章; 市居修; 伊東拓也; 今内覚; 大橋和彦, 日本獣医学会学術集会講演要旨集, 159回, 399, 399, Aug. 2016
  (公社)日本獣医学会, Japanese
• 犬と猫の慢性腎臓病における尿細管周囲毛細血管の変化
  中村麗菜; 矢吹映; 市居修; 水川葉月; 横山望; 大和修, 日本獣医学会学術集会講演要旨集, 159回, 462, 462, Aug. 2016
  (公社)日本獣医学会, Japanese
• 3D‐SEM法を利用した腎生検の迅速電子顕微鏡診断への応用
  高木孝士; 城謙輔; 大野伸彦; 大野伸彦; 齊藤成; THAI Quynh T; 昆泰寛; 市居修; YESER Alewa H; 笹野公伸, 日本病理学会会誌, 105, 1, 369, 369, Apr. 2016
  (一社)日本病理学会, Japanese
• “Sex-related difference of the mediastinal fat associated lymphoid clusters in MRL/MpJ-Faslpr autoimmune disease model mice.”
  Y. H. A. Elewa; O. Ichii; Y. Kon, The 11th Scientific Vet. Med. (second international) conference. El-Ain-El Sokhna, Egypt., 11th, Mar. 2016
• Rapid specimen preparation to improve throughput of electron microscopic volume imaging
  THAI Truc Quynh; NGUYEN Huy Bang; SAITOH Sei; SAITOH Yurika; SHIMO Satoshi; ELEWA Yaser; Hosny Ali; ICHII Osamu; KON Yasuhiro; TAKAKI Takashi; JOH Kensuke; OHNO Nobuhiko, 日本顕微鏡学会関東支部講演会予稿集, 40th, 26, 2016
  English
• 鳥類における新規腎障害マーカーの探索
  石井千尋; 池中良徳; 市居修; 中山翔太; 西村紳一郎; 大橋哲; 田中誠一; 齊藤慶輔; 渡邊有希子; 水川葉月; 石塚真由美, 日本野生動物医学会大会・講演要旨集, 22nd, 123, 2016
  Japanese
• メタボローム解析を用いたイエネコにおけるPCBsの毒性影響評価
  西川博之; 野見山桂; 水川葉月; 横山望; 市居修; 滝口満喜; 石塚真由美; 池中良徳; 中山翔太; 江口哲史; 国末達也; 田辺信介, 環境化学討論会要旨集(CD-ROM), 25th, ROMBUNNO.1A‐12, 2016
  Japanese
• ネコにおけるポリ塩化ビフェニル(PCBs)の異物代謝機構解明と甲状腺ホルモンへの影響評価
  水川葉月; 前原美咲; 横山望; 市居修; 滝口満喜; 野見山桂; 西川博之; 池中良徳; 中山翔太; 高口倖暉; 田辺信介; 石塚真由美, 環境化学討論会要旨集(CD-ROM), 25th, ROMBUNNO.1A‐13, 2016
  Japanese
• In vivo analysis of metabolic capacities and effects on the thyroid hormone and in cats treated with polychlorinated biphenyls
  MIZUKAWA Hazuki; MAEHARA Misaki; YOKOYAMA Nozomu; ICHII Osamu; TAKIGUCHI Mitsuyoshi; NOMIYAMA Kei; NISHIKAWA Hiroyuki; IKENAKA Yoshinori; NAKAYAMA Shouta M.M.; TAKAGUCHI Kohki; TANABE Shinsuke; ISHIZUKA Mayumi, Annual Meeting of the Japanese Society of Toxicology, 43.1, Suppl., P-6, S215, Jun. 2016
  ポリ塩化ビフェニル（PCBs）の水酸化代謝物であるOH-PCBsは、肝臓内で薬物代謝酵素より生成され、その後体外へ排泄される。しかしながら、一部の水酸化代謝物は甲状腺ホルモン（TH）と類似の構造をもつため、THの恒常性を撹乱することが危惧されている。これまでに、多様な陸棲哺乳類の血中OH-PCBsを分析したところ、種間でOH-PCBsの組成に差異が認められ、中でも、ネコのOH-PCBs残留パターンは他種と大きく異なることから、本種は特異な代謝機能を有することが示唆された。しかし、ネコの異物代謝能の研究は僅かであり、化学物質暴露による毒性影響も不明な点が多い。本研究では、ネコにおけるPCBs <i>in vivo</i>暴露試験を実施し、体内動態および代謝に関与する酵素活性や遺伝子を解析するとともに、化学物質の暴露評価に繋がる基盤的情報の収集を目的とした。<br>コーン油に溶解した12異性体のPCBsを腹腔内投与し、経時採血した血清中PCBsおよびOH-PCBs濃度について同条件で実施したイヌの<i>in vivo</i>試験と比較した結果、異性体の残留パターンや体内動態にイヌとネコで種差が観察された。とくにネコでは低塩素化体の残留が顕著であった。また、代謝酵素活性および遺伝子解析の結果、PCBs暴露によりEROD、MROD、PROD活性は上昇するものの、第2相抱合酵素（UGTやSULT）活性は変化せず、PCBs暴露による抱合酵素活性への影響もみられなかった。また、<i>CYP1A1</i>および<i>CYP1A2</i>遺伝子の発現量の上昇も認められた。<br> 本研究により、ネコのPCBs吸収・代謝・排泄能はイヌと異なることが示唆され、とくに低塩素化OH-PCBsの毒性リスクは高いことが予想された。低塩素化OH-PCBsは血中でTH輸送タンパクとの競合結合や、THの硫酸抱合排泄の阻害、TH起因性遺伝子の転写抑制などが報告されており、ネコの甲状腺機能障害が懸念される。, The Japanese Society of Toxicology, Japanese
• 自己免疫疾患モデルMRL/MpJ‐Fas^lprマウスにみられる卵子のキャッチアップ障害
  細谷実里奈; 中村鉄平; 中村鉄平; HOSNY Ali Elewa Yaser; HOSNY Ali Elewa Yaser; 市居修; 昆泰寛, 日本獣医学会学術集会講演要旨集, 159th, 314, 314, 2016
  (公社)日本獣医学会, Japanese
• 生体内組織形成術による管状組織体の壁厚制御
  寺澤武; 寺澤武; 西邑隆徳; 三谷朋弘; 市居修; 中山泰秀; 中山泰秀, 人工臓器(日本人工臓器学会), 45, 2, S.129, 129, 2016
  (一社)日本人工臓器学会, Japanese
• マウス十二指腸空腸曲の細胞外基質は屈曲形成と共に変遷する
  尾之内佐和; 市居修; 昆泰寛, 日本獣医学会学術集会講演要旨集, 158回, 277, 277, Aug. 2015
  (公社)日本獣医学会, Japanese
• 遠位尿細管傷害を知らせるマーカー分子“IL‐1F6/IL‐36a”の有用性
  市居修; 中村鉄平; 中村鉄平; 堀野太郎; 木村純平; 岡村匡史; 昆泰寛, 日本獣医学会学術集会講演要旨集, 158回, 276, 276, Aug. 2015
  (公社)日本獣医学会, Japanese
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるToll‐like receptor2,4の局在解析
  横山望; 大田寛; 賀川由美子; 山崎淳平; 市居修; KHOIRUN Nisa; 森田智也; 大菅辰幸; 佐々木東; 森下啓太郎; 中村健介; 滝口満喜, 日本獣医学会学術集会講演要旨集, 158回, 402, 402, Aug. 2015
  (公社)日本獣医学会, Japanese
• コットンラットの咽頭喉頭部,甲状腺及びその周囲組織にみられる形態異常
  中村鉄平; 中村鉄平; 入江隆夫; 市居修; 篠原明夫; 浦口宏二; 越本知大; 長崎健一; 昆泰寛, 日本獣医学会学術集会講演要旨集, 158回, 274, 274, Aug. 2015
  (公社)日本獣医学会, Japanese
• A Novel Mouse Mediastinal Fat-associated Lymphoid Tissue and Its Relationship with Autoimmune Disease
  Y. H. A. Elewa; O. Ichii; S. Otsuka-Kanazaw; Y. Kon, The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA). Bali, Indonesia, 5th, Feb. 2015
  English
• ケージ交換時のハンドリングは実験用マウスの表現型に影響を与えるか?
  小野麻衣子; 佐々木隼人; 長崎健一; 鳥越大輔; 市居修; 佐々木宣哉; 安居院高志, 日本実験動物技術者協会総会講演要旨集, 49th, 59, 2015
  Japanese
• 細胞形態ダイナミクスをリードする分子群を求めて miR-26a 腎糸球体上皮細胞の機能形態を制御する短鎖RNA
  市居 修, 日本獣医学会学術集会講演要旨集, 157回, 217, 217, Aug. 2014
  (公社)日本獣医学会, Japanese
• 新生子MRL/MpJマウスにおける卵巣内肥満細胞の由来 卵巣-卵素采結合部から出現する可能性
  中村 鉄平; 千原 正尚; 市居 修; 大塚 沙織; 長崎 健一; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 157回, 320, 320, Aug. 2014
  (公社)日本獣医学会, Japanese
• マウス縦隔の脂肪関連リンパ球集積の性状解析(Characterization of mouse mediastinal fat-associated lymphoid clusters)
  Elewa Yaser Hosny Ali; 市居 修; 大塚 沙織; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 157回, 330, 330, Aug. 2014
  (公社)日本獣医学会, English
• Greb1―DBA/2Crマウスに出現する遠位尿細管由来嚢胞の原因遺伝子候補―
  市居修; 大塚沙織; 矢吹映; 堀野太郎; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 157回, 328, 328, Aug. 2014
  (公社)日本獣医学会, Japanese
• Toll‐like receptor 8は自己免疫性糸球体腎炎の足細胞傷害に関与する
  木村純平; 市居修; 大田寛; 中村鉄平; 堀野太郎; 大塚沙織; 昆泰寛, 日本獣医学会学術集会講演要旨集, 157回, 323, 323, Aug. 2014
  (公社)日本獣医学会, Japanese
• 自己免疫疾患モデルMRL/MpJ‐lprマウスの卵巣は機能形態異常を示す
  大谷祐紀; 市居修; 大塚沙織; 中村鉄平; 千原正尚; 昆泰寛, 日本獣医学会学術集会講演要旨集, 157回, 326, 326, Aug. 2014
  (公社)日本獣医学会, Japanese
• マウス十二指腸空腸曲における屈曲内‐外側の形態学的差異
  尾之内佐和; 市居修; 大塚沙織; 昆泰寛, 日本獣医学会学術集会講演要旨集, 157回, 325, 325, Aug. 2014
  (公社)日本獣医学会, Japanese
• MRL/MpJマウスに出現する精巣内卵細胞のエピゲノム解析
  大塚沙織; 市居修; 昆泰寛, 日本獣医学会学術集会講演要旨集, 157回, 319, 319, Aug. 2014
  (公社)日本獣医学会, Japanese
• 腎障害時にみられるMRL/MpJマウスの特別な修復過程について
  塩水流大地; 市居修; 中村鉄平; 大塚沙織; 昆泰寛, 日本獣医学会学術集会講演要旨集, 157回, 324, 324, Aug. 2014
  (公社)日本獣医学会, Japanese
• miR‐26a―腎糸球体上皮細胞の機能形態を制御する短鎖RNA
  市居修, 日本獣医学会学術集会講演要旨集, 157th, 217, 217, 2014
  (公社)日本獣医学会, Japanese
• 新生子MRL/MpJマウスにおける卵巣内肥満細胞の由来―卵巣‐卵巣采結合部から出現する可能性―
  中村鉄平; 千原正尚; 市居修; 大塚沙織; 長崎健一; 昆泰寛, 日本獣医学会学術集会講演要旨集, 157th, 320, 320, 2014
  (公社)日本獣医学会, Japanese
• ビタミンA欠乏食給餌マウスからみる精細胞‐血液精巣関門の相互作用
  千原正尚; 市居修; 大塚沙織; 昆泰寛, 日本獣医学会学術集会講演要旨集, 156回, 200, 200, Aug. 2013
  (公社)日本獣医学会, Japanese
• マウス慢性膵炎の系統差に関する研究―MRLマウス1番染色体テロメア領域の影響―
  岡田祐樹; 市居修; 大塚沙織; 昆泰寛, 日本獣医学会学術集会講演要旨集, 156回, 195, 195, Aug. 2013
  (公社)日本獣医学会, Japanese
• MRL/MpJマウスの卵細胞発育を制御する遺伝学的因子
  山下由真; 市居修; 大塚沙織; 昆泰寛, 日本獣医学会学術集会講演要旨集, 156回, 198, 198, Aug. 2013
  (公社)日本獣医学会, Japanese
• 伴侶動物の腎臓に発現するmiRNAのデータベース構築―動物種共通のバイオマーカー開発を目指して―
  市居修; 大塚沙織; 矢吹映; 大田寛; 堀野太郎; 昆泰寛, 日本獣医学会学術集会講演要旨集, 156回, 193, 193, Aug. 2013
  (公社)日本獣医学会, Japanese
• Ovarian cysts in MRL/MpJ mice are derived from the extraovarian rete: a developmental study (vol 219, pg 743, 2011)
  Shin-Hyo Lee; Osamu Ichii; Saori Otsuka; Yaser Hosny Ali Elewa; Yuka Namiki; Yoshiharu Hashimoto; Yasuhiro Kon, JOURNAL OF ANATOMY, 222, 3, 396, 396, Mar. 2013
  English, Others
• 糸球体病変を制御するmiRNAの探索―動物種共通のバイオマーカー開発を目指して―
  市居修; 大塚沙織; 中村鉄平; 野元由佳; 橋本善春; 堀野太郎; 昆泰寛, 日本獣医学会学術集会講演要旨集, 155回, 188, 188, Mar. 2013
  (公社)日本獣医学会, Japanese
• 熱ショック誘導性の精細管腔内石灰沈着の発生機序と抑制因子の解析
  千原正尚; 市居修; 大塚沙織; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 155回, 188, 188, Mar. 2013
  (公社)日本獣医学会, Japanese
• MRLマウス肥満細胞は新生子期の卵形成に関与する
  中村鉄平; 市居修; 大塚沙織; 坂田侑子; 長崎健一; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 155回, 188, 188, Mar. 2013
  (公社)日本獣医学会, Japanese
• 糸球体腎炎モデルマウスにおける足細胞傷害の解析
  木村純平; 市居修; 大塚沙織; 佐々木隼人; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 155回, 187, 187, Mar. 2013
  (公社)日本獣医学会, Japanese
• Basic nephrology 分子生物学 腎臓病のmiRNA解析
  市居 修, Annual Review腎臓, 2013, 80, 87, Jan. 2013
  (株)中外医学社, Japanese
• マウス消化管の発生に関する研究~どうして腸管は曲がるのか?~
  尾之内佐和; 市居修; 大塚沙織; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 154回, 173, 173, Aug. 2012
  (公社)日本獣医学会, Japanese
• 高温曝露後の精巣における減数分裂の進行と精細管腔内に出現する石灰沈着
  千原正尚; 市居修; 大塚沙織; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 154回, 176, 176, Aug. 2012
  (公社)日本獣医学会, Japanese
• 尿毒症物質インドキシル硫酸は腎糸球体上皮細胞傷害に関与する―芳香族炭化水素受容体のリガンド作用―
  市居修; 若新英史; 村上太一; 堀野太郎; LU Huiyan; TAKAHASHI Hideko; PATRICIA Zerfas; 大塚沙織; 橋本善春; 昆泰寛; KOPP Jeffrey, 日本獣医学会学術集会講演要旨集, 154回, 176, 176, Aug. 2012
  (公社)日本獣医学会, Japanese
• マレック病感染鶏由来腫瘍病変部における免疫抑制因子PD‐1/PD‐L1発現の形態学的解析
  村田史郎; 松山あゆ美; 伊勢崎政美; 高崎紗蘭; 市居修; 今内覚; 大橋和彦, 日本獣医学会学術集会講演要旨集, 154回, 234, 234, Aug. 2012
  (公社)日本獣医学会, Japanese
• 節足動物DNAのリボゾームRNA遺伝子ITS領域のサイズ分析による生物種同定の試み
  佐藤 福太郎; 遠藤 大二; 吉村 貴行; 伊東 拓也; 長 雄一; 浅川 満彦; 昆 泰寛; 市居 修; 大塚 沙織; 林 正信, 獣医寄生虫学会誌, 10, 1-2, 50, 50, Mar. 2012
  日本獣医寄生虫学会, Japanese
• MRL/MpJマウスの卵巣内肥満細胞出現に関与する遺伝的因子の解析
  中村鉄平; 坂田侑子; 大塚沙織; 市居修; 並木由佳; 長崎健一; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 153回, 194, 194, Mar. 2012
  (公社)日本獣医学会, Japanese
• 糸球体腎炎モデルマウスにおける尿中脱落細胞の動態解析
  木村純平; 市居修; 大塚沙織; 金澤智則; 並木由佳; 橋本善春; 昆泰寛, 解剖学雑誌, 87, 1, 5, 5, Mar. 2012
  (一社)日本解剖学会, Japanese
• マウス精巣における密着結合蛋白claudin‐3の局在および機能解析
  千原正尚; 市居修; 大塚沙織; 並木由佳; 池淵良洋; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 153回, 194, 194, Mar. 2012
  (公社)日本獣医学会, Japanese
• High-Resolution Linkage Mapping of the Rat Hooded Locus
  D. Torigoe; O. Ichii; D. Ruihua; T. Ohnaka; S. Okano; Y. Kon; N. Sasaki; T. Agui, JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE, 50, 5, 793, 793, Sep. 2011
  English, Summary international conference
• マウス涙腺炎における涙液を使った病態解析
  小千田圭吾; 市居修; 大塚沙織; 並木由佳; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 152回, 175, 175, Aug. 2011
  (公社)日本獣医学会, Japanese
• MRL/MpJマウスにおける精巣内卵細胞への分化開始時期の特定
  大塚沙織; 市居修; 佐々木宣哉; 並木由佳; 橋本善春; 遠藤大二; 昆泰寛, 日本獣医学会学術集会講演要旨集, 152回, 168, 168, Aug. 2011
  (公社)日本獣医学会, Japanese
• マウス新生子卵巣に出現する肥満細胞の系統差解析
  中村鉄平; 市居修; 大塚沙織; 並木由佳; 坂田侑子; 長崎健一; 服部秀樹; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 152回, 172, 172, Aug. 2011
  (公社)日本獣医学会, Japanese
• マウス心筋発生過程に出現するTUNEL陽性細胞の解析
  長沼佑季; 市居修; 大塚沙織; 並木由佳; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 152回, 175, 175, Aug. 2011
  (公社)日本獣医学会, Japanese
• 129+Ter/Svマウスの遺伝的背景は腎糸球体硬化症に抵抗性である
  西野 智博; 佐々木 宣哉; 長崎 健一; 市居 修; 昆 泰寛; 安居院 高志, 日本獣医学会学術集会講演要旨集, 151回, 276, 276, Mar. 2011
  (公社)日本獣医学会, Japanese
• マウス血液精巣関門関連分子の局在と動態―claudin‐3,‐11,occludin,ZO‐1の発現解析―
  千原正尚; 市居修; 大塚沙織; 橋本善春; 昆泰寛, 解剖学雑誌, 86, 1, 12, 12, Mar. 2011
  (一社)日本解剖学会, Japanese
• MRLマウス新生子卵巣における肥満細胞の出現
  中村鉄平; 市居修; 大塚沙織; 並木由佳; 長崎健一; 服部秀樹; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 151回, 190, 190, Mar. 2011
  (公社)日本獣医学会, Japanese
• 腎症候性出血熱の致死的感染モデルの開発とその病態解析
  瀬戸隆弘; 吉川佳佑; 真田崇弘; SAASA Ngonda; 尾崎由佳; 市居修; 好井健太朗; 昆泰寛; 苅和宏明, 日本獣医学会学術集会講演要旨集, 151回, 245, 245, Mar. 2011
  (公社)日本獣医学会, Japanese
• 慢性腎臓病におけるmicroRNAの発現解析―腎間質病変に関与するmi‐R146a―
  市居修; 大塚沙織; 並木由佳; 佐々木宣哉; 橋本善春; 遠藤大二; 昆泰寛, 日本獣医学会学術集会講演要旨集, 151回, 189, 189, Mar. 2011
  (公社)日本獣医学会, Japanese
• 129^+Ter/Svマウスの遺伝的背景は腎糸球体硬化症に抵抗性である
  西野智博; 佐々木宣哉; 長崎健一; 市居修; 昆泰寛; 安居院高志, 日本獣医学会学術集会講演要旨集, 151回, 276, 276, Mar. 2011
  (公社)日本獣医学会, Japanese
• MRL/MpJマウスにおける卵巣網由来卵巣嚢腫の経時的観察
  李愼曉; 大塚沙織; 市居修; 並木由佳; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 151回, 190, 190, Mar. 2011
  (公社)日本獣医学会, Japanese
• 節足動物DNAのITS領域サイズ分析による生物種同定の試み
  遠藤大二; 吉村貴行; 佐藤福太郎; 伊東拓也; 長雄一; 浅川満彦; 昆泰寛; 市居修; 大塚沙織; 林正信, 日本獣医学会学術集会講演要旨集, 151st, 220, 2011
  Japanese
• ラット頭巾斑遺伝子座の遺伝学的解析
  鳥越大輔; 市居修; 党瑞華; 佐々木宣哉; 昆泰寛; 安居院高志, 日本実験動物学会総会講演要旨集, 58th, 173, 2011
  Japanese
• 節足動物DNAのリボゾームRNA遺伝子ITS領域のサイズ分析による生物種同定の試み
  佐藤福太郎; 遠藤大二; 吉村貴行; 伊東拓也; 長雄一; 浅川満彦; 昆泰寛; 市居修; 大塚沙織; 林正信, 日本獣医学会学術集会講演要旨集, 152nd, 207, 2011
  Japanese
• Cytoarchitectural differences of myoepithelial cells among goat major salivary glands (vol 34, pg 557, 2010)
  Yaser Hosny Elewa; Mohammad Hafez Bareedy; Ahmed Awad Abuel-Atta; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Shin-Hyo Lee; Yoshiharu Hashimoto; Yasuhiro Kon, VETERINARY RESEARCH COMMUNICATIONS, 34, 8, 727, 727, Dec. 2010
  English, Others
• ヤギの大唾液腺における離乳前・後期のアクアポリンファミリー発現の比較解析(Comparative analysis of the expression of aquaporin family between pre- and post-weaning periods in goat major salivary glands)
  Yaser Elewa; 市居 修; 大塚 沙織; 金澤 智則; 李 愼曉; 並木 由佳; 昆 泰寛; 橋本 善春, 日本獣医学会学術集会講演要旨集, 150回, 160, 160, Sep. 2010
  (公社)日本獣医学会, English
• MRLマウスの精巣内卵細胞出現に関与する遺伝的因子の解析
  大塚沙織; 市居修; 佐々木宣哉; 並木由佳; 橋本善春; 遠藤大二; 昆泰寛, 日本獣医学会学術集会講演要旨集, 150回, 154, 154, Sep. 2010
  (公社)日本獣医学会, Japanese
• DBA/2マウスの腎臓における遠位尿細管上皮傷害―多発性嚢胞腎の早期病変としての可能性
  市居修; 大塚沙織; 矢吹映; 佐々木宣哉; 並木由佳; 橋本善春; 遠藤大二; 昆泰寛, 日本獣医学会学術集会講演要旨集, 150回, 160, 160, Sep. 2010
  (公社)日本獣医学会, Japanese
• 尿中脱落細胞の動態は腎疾患の増悪と相関する―糸球体腎炎モデルの解析
  木村純平; 市居修; 大塚沙織; 金澤智則; 並木由佳; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 150回, 160, 160, Sep. 2010
  (公社)日本獣医学会, Japanese
• ヤギ耳下腺の年齢関連の構造変化(Age-related structural changes of parotid salivary glands in goat)
  Elewa Yaser; 橋本 善春; 市居 修; 大塚 沙織; 金澤 智則; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 149回, 211, 211, Mar. 2010
  (公社)日本獣医学会, English
• マウスのネフロン形成過程における間葉‐上皮転換(MET)の解析
  金澤智則; 大塚沙織; 市居修; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 149回, 216, 216, Mar. 2010
  (公社)日本獣医学会, Japanese
• イヌおよびネコの腎疾患における病態差―腎機能とスリット膜関連分子の動態―
  市居修; 矢吹映; 佐々木宣哉; 大田寛; 滝口満喜; 大塚沙織; 橋本善春; 遠藤大二; 昆泰寛, 日本獣医学会学術集会講演要旨集, 149回, 213, 213, Mar. 2010
  (公社)日本獣医学会, Japanese
• MRLマウス胎子精巣内における減数分裂開始と精巣内卵細胞の出現
  大塚沙織; 市居修; 佐々木宣哉; 橋本善春; 遠藤大二; 昆泰寛, 日本獣医学会学術集会講演要旨集, 149回, 213, 213, Mar. 2010
  (公社)日本獣医学会, Japanese
• (DBA/2×C57BL/6)F2交雑マウスに出現する水腎症
  市居 修; 昆 泰寛; 佐々木 宣哉; 矢吹 映; 大塚 沙織; 橋本 善春; 遠藤 大二, 日本獣医学会学術集会講演要旨集, 148回, 153, 153, Sep. 2009
  (公社)日本獣医学会, Japanese
• マウス精子形成過程における血液精巣関門構成分子の動態
  千原正尚; 大塚沙織; 市居修; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 148回, 155, 155, Sep. 2009
  (公社)日本獣医学会, Japanese
• 腎疾患の新しい診断指標―Interleukin 1 family,member 6は尿細管傷害マーカーになり得る―
  市居修; 昆泰寛; 佐々木宣哉; 橋本善春; 大田寛; 滝口満喜; 矢吹映; 遠藤大二, 日本獣医学会学術集会講演要旨集, 147回, 79, 79, Mar. 2009
  (公社)日本獣医学会, Japanese
• (DBA/2x C57BL/6)F2交雑マウスに出現する水腎症
  市居修; 昆泰寛; 佐々木宣哉; 矢吹映; 大塚沙織; 橋本善春; 遠藤大二, 日本獣医学会学術集会講演要旨集, 148th, 153, 153, 2009
  (公社)日本獣医学会, Japanese
• MRL/MpJマウスに出現する精巣内卵細胞の解析
  大塚沙織; 市居修; 佐々木宣哉; 橋本善春; 遠藤大二; 昆泰寛, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2009, 11, 2009
  Japanese
• マウス精子形成過程における血液精巣関門構成分子の動態
  千原正尚; 市居修; 大塚沙織; 橋本善春; 昆泰寛, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2009, 12, 2009
  Japanese
• 慢性腎臓病は制御可能か?―疾患モデル動物にターゲット因子を求めて―
  市居修, 北海道獣医師会雑誌, 53, 11, 638, 644, Nov. 2009
  (公社)北海道獣医師会, Japanese
• テンシン2遺伝子の糸球体上皮細胞における機能解析
  佐々木宣哉; 市居修; 昆泰寛; 安居院高志, 日本獣医学会学術集会講演要旨集, 146回, 300, 300, Sep. 2008
  (公社)日本獣医学会, Japanese
• Interferon activated gene200(Ifi200)遺伝子群と自己免疫性糸球体腎炎の関連性
  市居修; 上川昭博; 佐々木宣哉; 今野明弘; 遠藤大二; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 146回, 147, 147, Sep. 2008
  (公社)日本獣医学会, Japanese
• 性腺摘出が自己免疫性糸球体腎炎の病態に与える影響について
  市居修; 今野明弘; 佐々木宣哉; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 145回, 161, 161, Mar. 2008
  (公社)日本獣医学会, Japanese
• プロダクトA 産業ビジネス分野におけるオブジェクト指向RFIDシステムのご提案
  市居 英治; 神田 修, 自動認識 / 月刊自動認識編集委員会 編, 20, 9, 43, 46, Aug. 2007
  東京 : 日本工業出版, Japanese
• 自己免疫性糸球体腎炎とFc gamma receptor III(FcγRIII)の関連性について
  市居修; 今野明弘; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 144回, 33, 33, Aug. 2007
  (公社)日本獣医学会, Japanese
• A Study on the Information System Using Radio Frequency Identification
  SHIBAYAMA Akihiro; TAKIZAWA Osamu; HOSOKAWA Masafumi; ICHII Tuguyuki; HISADA Yoshiaki; MURAKAMI Masahiro, Journal of Social Safety Science, 8, 135, 144, 23 Nov. 2006, [Peer-reviewed]
  A portable information mutual system for assisting rescue personnel in disaster situations has been developed. In terms of proceeding the system, Radio Frequency Identification (RFID) tags, which contain information such as bulding structure or family, are set to buldings. The system is used for the cases of usual condition, a fire, disaster, judgement of damage level and the support of damage recovery. RFID's information can be remotely downloaded or uploaded by portable microwave terminals. This can help safety assessment for inspectors on condition of refugee's search or the judgement of damaged buildings., Institute of Social Safety Science, Japanese
• 齧歯目における腎臓の短および長ループネフロンの比率の種差(Species Specific Differences in the Ratio of Short to Long Loop Nephrons in the Kidneys of Laboratory Rodents)
  市居 修; 矢吹 映; 小嶋 敏慶; 松元 光春; 鈴木 秀作, Experimental Animals, 55, 5, 473, 476, Oct. 2006
  English
• 齧歯目における腎臓の短および長ループネフロンの比率の種差(Species Specific Differences in the Ratio of Short to Long Loop Nephrons in the Kidneys of Laboratory Rodents)
  市居 修; 矢吹 映; 小嶋 敏慶; 松元 光春; 鈴木 秀作, Experimental Animals, 55, 5, 473, 476, Oct. 2006, [Peer-reviewed], [Domestic magazines]
  English
• MRL/MpJマウス由来コンジェニックマウスに出現する自己免疫性糸球体腎炎
  市居修; 今野明弘; 橋本善春; 昆泰寛, 日本獣医学会学術集会講演要旨集, 142回, 40, 40, Aug. 2006
  (公社)日本獣医学会, Japanese
• 齧歯目の腎臓の組織構造における種差(Rodent Renal Structure Differs among Species)
  市居 修; 矢吹 映; 小嶋 敏慶; 松元 光春; 鈴木 秀作, The Journal of Veterinary Medical Science, 68, 5, 439, 445, May 2006
  English
• Rodent renal structure differs among species
  O Ichii; A Yabuki; T Ojima; M Matsumoto; S Suzuki, JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, 5, 439, 445, May 2006
  English
• 齧歯目の腎臓における種差 ネフロン長,GFR調節因子,水チャネルおよびナトリウムポンプについて
  市居 修; 矢吹 映; 小嶋 敏慶; 松元 光春; 鈴木 秀作, 日本獣医学会学術集会講演要旨集, 140回, 46, 46, Aug. 2005
  (公社)日本獣医学会, Japanese
• げっ歯目の腎臓における種差‐ネフロン長,GFR調節因子,水チャネルおよびナトリウムポンプについて‐
  市居修; 矢吹映; 小嶋敏慶; 松元光春; 鈴木秀作, 日本獣医学会学術集会講演要旨集, 140th, 46, 46, 2005
  (公社)日本獣医学会, Japanese

• イヌとネコの腎泌尿器病学
  日本獣医腎泌尿器学会, 泌尿器の発生とその異常および解剖・組織
  ファームプレス, Oct. 2024, [Contributor]
• HORMONE FRONTIER IN GYNECOLOGY
  大谷祐紀; 市居修; 昆泰寛, 特集 生殖内分泌学と生殖免疫学の接点、種横断的な知見から考える精巣と免疫のかかわり
  メディカルレビュー社, Sep. 2023, [Others]
• 獣医組織学第9版
  日本獣医解剖学会, 第12章「腎臓」、第19章「家禽の組織学
  学窓社, Mar. 2023, 9784873627878, 439p, Japanese, [Contributor]
• 腎臓内科第16巻第2号、特集 脊椎動物の進化と腎臓、イヌとネコの腎臓とその病態
  市居修, 170
  科学評論社, Aug. 2022, [Contributor]
• 獣医組織学第8版
  日本獣医解剖学会, 第12章腎臓、第19章家禽の組織学・泌尿器
  学窓社, Mar. 2020, 9784873627731, xviii, 397p, Japanese, [Joint editor]
• 新生子MRL/MpJマウスにおいて卵巣マスト細胞は卵巣-卵巣采結合部の方向に遊走する : 局在変化が明らかにするマスト細胞の新たな役割 (特集 マスト細胞研究の新展開)
  中村鉄平; 市居修; Yaser Hosny Ali Elewa; 昆泰寛
  臨床免疫・アレルギー科、科学評論社, 2019
• A possibility of the iBTA tissues derived from animals for off-the-shelf biomaterials
  佐藤康史, 岩井良輔, 西邑隆徳, 市居修, 宮本伸二, 秋吉秀保, 中山泰秀
  日本人工臓器学会 株式会社秀潤社, 2019, [Joint work]
• 牛の乳房炎
  日本乳房炎研究会編, 第3章菌がつくるバイオフィルムとは
  緑書房, Nov. 2018, [Joint work]
• 獣医組織学第7版
  日本獣医解剖学会編, 第12章「腎臓」、第19章「家禽の組織学」
  学窓社, 17 Mar. 2017, [Contributor]
• カラーアトラス獣医解剖学第2版
  カラーアトラス獣医解剖学編集委員会, 第12章「鳥類の解剖学」
  緑書房, 20 Mar. 2016, [Joint translation]
• 猫の解剖 カラーリングアトラス
  九郎丸正道監修, 「泌尿器」、「呼吸器」
  学窓社, 2014, [Joint translation]
• 獣医組織学第6版
  日本獣医解剖学会編, 第12章「腎臓」、第19章「家禽の組織学」
  学窓社, 2014, [Contributor]
• 小動物の実践歯科学
  Brool A. Niemiec, 第1章 解剖学と生理学
  緑書房, Mar. 2013, [Joint translation]
• Annual Review 腎臓
  富野康日己ら編, C分子生物学 腎臓病のmiRNA解析
  中外医学社, Jan. 2013, [Contributor]
• 獣医解剖・組織・発生学
  日本獣医解剖学会編, 獣医解剖学 第10章 泌尿器系
  学窓社, May 2012, [Contributor]
• 獣医組織学第5版
  日本獣医解剖学会編, 第12章「腎臓」
  学窓社, Mar. 2011, [Contributor]

• コラーゲンの質を可視化する：変性コラーゲンと総コラーゲンのin situ多重染色法
  中村 鉄平; 市居修; 鷲見 嘉奈子; 吉良 俊之介; 森松 正美
  第167回日本獣医学会学術集会, 12 Sep. 2024
• 尿路上皮バリア異常モデルマウスにみられる急性腎障害
  市居修; 岡村匡史; 大谷祐紀; 中村 鉄平
  第167回日本獣医学会学術集会, 11 Sep. 2024
• ネコの胆膵管合流部にみられる解剖学的特徴の精査と三臓器炎模倣の試み
  久保田 和基; 大谷 祐紀; 平石 真也; 大江 紗央; 市居 修
  第167回日本獣医学会学術集会, 10 Sep. 2024
• 17型コラーゲンの全身性欠損がマウス泌尿器の生後発達に与える影響について
  酒々井 エイミー; 大江 紗央; 平石 真也; 夏賀 健; 大谷 祐紀; 市居 修
  第167回日本獣医学会学術集会, 10 Sep. 2024
• 結石により閉塞したネコ尿管におけるアドレナリンα1受容体発現の変化
  大江紗央; 小山田和央; 青木 大; 大谷祐紀; 市居 修
  第167回日本獣医学会学術集会, 10 Sep. 2024
• ネコの近位尿細管におけるO結合型糖鎖修飾とその病態変化
  吉良俊之介; 大江紗央; 平石真也; 日尾野隆大; 大谷祐紀; 市居 修
  第167回日本獣医学会学術集会, 10 Sep. 2024
• Morphological sex-related differences found in the rectal mucosa-associated lymphoid tissues of C57BL/6NCrSlc mice
  Rubel MZU; Namba T; Masum MA; Hiraishi M; Kon Y; Ichii O
  The 4th Join meeting of Veterinary Science in East Asia, 09 Sep. 2024
• Expression of adrenoceptor alpha 1 in the obstructed ureters of feline urolithiasis cases
  Sao OE; Md. Zahir; Uddin RUBEL; Masaya HIRAISHI; Kazuhisa OYAMADA; Hiroshi AOKI; Yuki OTANI; Teppei NAKAMURA; Osamu ICHII
  The 12th Sapporo summer symposium for one health, 04 Sep. 2024
• ネコ近位直尿細管上皮細胞の特徴 － ビオチン蓄積とミトコンドリアの形態変化について －
  吉良俊之介; 大江紗央; 平石真也; Rubel Md. Zahir Uddin; 大谷祐紀; 藏原なつめ; 矢吹 映; 市居 修
  第16回日本獣医腎泌尿器学会学術集会, 25 Aug. 2024
• 吉良俊之介, 市居修, 平石真也, 昆泰寛
  ネコの近位尿細管上皮細胞に見出した新たな細胞学的特徴
  第4回⽇本獣医解剖アカデミア, 25 Mar. 2024
• 涙液産生不全モデルマウスの眼関連リンパ組織
  平石真也; 市居修; 昆泰寛
  第4回⽇本獣医解剖アカデミア, 25 Mar. 2024
• 乳廃用牛の医療応用－生体内で形成された結合組織体の機械特性評価
  柳瀬圭太; 市居 修; 中村鉄平; 海田こころ; 片山由佳; 池田哲平; 平石真也; 新川祐希; 小川美朱; 秋吉秀保; 西邑隆徳
  第45回日本バイオマテリアル学会大会, 06 Nov. 2023
• 乳廃用牛の医療応用－皮下埋植した鋳型内の低酸素環境は結合組織体の形成を誘導する
  中村鉄平; 市居 修; 柳瀬圭太; 池田哲平; 平石真也; 片山由佳; 海田こころ; 新川祐希; 小川美朱; 辻絵里香; 秋吉秀保; 森松正美; 西邑隆徳
  第45回日本バイオマテリアル学会大会, 06 Nov. 2023
• Histological characteristics of rectum mucosa-associated lymphoid tissues in auto immune disease-prone MRL/MpJ-Faslpr/lpr mice
  Rubel MZU; Ichii O; Namba T; Masum MA; Kon Y
  The 11th Sapporo summer symposium for one health (SaSSOH), 14 Sep. 2023
• Immune cells constituting the rectal lymphoid tissues in MRL/MpJ-Faslpr/lpr mice
  Rubel MZU; Ichii O; Namba T; Masum MA; Kon Y
  第166回日本獣医学会学術集会, 05 Sep. 2023
• 薬物誘導型妊娠糖尿病モデルマウスの作出とその病態について
  高橋なるみ; 市居修; 大谷祐紀; 難波貴志; 平石真也; 中村鉄平; 昆泰寛
  第166回日本獣医学会学術集会, 05 Sep. 2023
• コットンラットの胸腺にみられる嚢胞の由来と胸腺機能との関連
  中村 鉄平; 市居 修; 入江 隆夫; 篠原 明男; 孝口 裕一; 昆 泰寛; 森松 正美
  第166回日本獣医学会学術集会, 05 Sep. 2023
• Roles of an inflammatory cytokine TL1A in gdT cell activation and psoriasis pathogenesis
  王 上一; 香西 美奈; Md. Zahir; Uddin Rubel; 平石 真也; 市居 修; 高田 健介; 稲葉 睦
  第166回日本獣医学会学術集会, 05 Sep. 2023
• 自己免疫疾患モデルマウス頭部にみられる粘膜上皮－免疫連関の変化
  平石真也; 市居修; 中村鉄平; 昆泰寛
  第166回日本獣医学会学術集会, 05 Sep. 2023
• 乳牛における子宮内細菌叢と受胎性との関連についての検討
  八木沢拓也; 内山淳平; 内山伊代; 安藤駿; 市居修; 村上裕信; 松下治; 片桐成二
  令和５年度獣医学術学会北海道地区, 31 Aug. 2023
• 薬物誘導型モデルマウスから考察する妊娠糖尿病と腎臓への影響
  高橋なるみ; 市居修; 大谷祐紀; 難波貴志; 平石真也; 中村鉄平; 昆泰寛
  第15回日本獣医腎泌尿器学会学術集会, 27 Aug. 2023
• 尿―上皮バリアの破綻と局所免疫応答
  市居修
  獣医アトピー・アレルギー・免疫学会 第15回ベーシックセミナー, 27 Aug. 2023, Invited oral presentation
  [Invited]
• ネコの腎臓をみなおす-腎臓病対策に有用な形態学的知見を求めて-
  吉良俊之介; 市居修; 難波貴志; 平石真也; 中村鉄平; 昆泰寛
  第15回日本獣医腎泌尿器学会学術集会, 27 Aug. 2023
• 腎泌尿器の発生、解剖および形態異常
  市居修
  第15回日本獣医腎泌尿器学会学術集会, 26 Aug. 2023, Public discourse
• Characteristics of lymphoid structures formed in the chicken ureters
  Md Zahir; Uddin Rubel; 市居修; 難波貴志; Md; Abdul Masum; 昆泰寛
  第3回⽇本獣医解剖アカデミア, 02 Apr. 2023
• 専門⽤語集の普及と今後の在り⽅ - 獣医組織学⽤語の改訂から -
  市居修; 昆泰寛
  第3回⽇本獣医解剖アカデミア, 01 Apr. 2023
  [Invited]
• 全身性自己免疫疾患モデルマウスにみられる頭部リンパ組織の形態変化
  平石真也; 市居修; 中村鉄平; 難波貴志; 昆泰寛
  第3回⽇本獣医解剖アカデミア, 01 Apr. 2023
• 尿－上皮バリアの恒常性を考える
  市居修
  第7回ポドサイト研究会, 18 Mar. 2023
  [Invited]
• 初回人工授精前の子宮内膜組織を用いた乳牛の低受胎に関わる子宮内細菌叢の解析
  八木沢拓也; 内山淳平; 内山伊代; 安藤駿; 市居修; 村上裕信; 松下治; 片桐成二
  第96回日本細菌学会, 16 Mar. 2023
• マダニ由来培養細胞に持続感染しているオルビウイルス
  松野啓太; 田谷友里恵; 市居修; 中尾亮; 布浦拓郎; 松尾栄子
  ウイルス学会, 13 Nov. 2022
• イヌとネコの尿中に出現するmicroRNAの意義
  市居修
  21st Federation of Asian Veterinary Associations (FAVA) Congress, 12 Nov. 2022, English
  [Invited]
• "Genital organ associated lymphoid tissue characteristics in cow, goat, and pig vaginal vestibule "
  Tsolmon Chuluunbaatar; Osamu Ichii; Takashi Namba; Md. Abdul Masum; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 8th Congress of Asian Association of Veterinary Anatomists (Asian AVA), 31 Oct. 2022
• Activity of Technical Workshop for Animal Gross Anatomy in the Japanese Association of Veterinary Anatomists
  Osamu Ichii; Motoharu Oishi; Yasuhiro Kon; Daisuke Kondoh; Hideshi Shibata; Makoto Sugiyama; Masashi Tsujio; Takuya Yokoyama; Takafumi Watanabe
  The 8th Congress of Asian Association of Veterinary Anatomists, 31 Oct. 2022
• Pathological role of hedgehog signaling in maintenance of the murine oviductal ciliated epithelium
  Hosotani M; Ichii O; Namba T; Nakamura T; Hasegawa Y; Masum MA; Watanabe T; Ueda H; Kon Y
  The 8th Congress of Asian Association of Veterinary Anatomists (Asian AVA), 30 Oct. 2022
• Altered morphologies of tear-secreting tissues and cornea in a mouse model of autoimmune disease.
  Masaya Hiraishi; Osamu Ichii; M; Abdul Masum、Ali Elewa; Yaser Hosny; Yasuhiro Kon
  The 8th Congress of Asian Association of Veterinary Anatomists, 30 Oct. 2022
• Characteristics of lymphoid tissue found in large intestine of chicken
  Rubel MZU; Ichii O; Namba T; Masum MA; Kon Y
  The 8th Congress of Asian Association of Veterinary Anatomists, 30 Oct. 2022
• Elucidation of vasculature-associated lymphoid tissue in caprine kidney
  Masum MA、Ichii O; Otani Y; Namba T; Kon Y
  The 8th Congress of Asian Association of Veterinary Anatomists, 30 Oct. 2022
• Genital organ associated lymphoid tissue characteristics in cow, goat, and pig vaginal vestibule
  Tsolmon Chuluunbaatar; Osamu Ichii; Takashi Namba; Md. Abdul Masum; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 8th Congress of Asian Association of Veterinary Anatomists, 30 Oct. 2022
• Lymphoid tissues found in mouse anorectal mucosa and systemic factors affecting their morphology
  Rubel MZU; Ichii O; Namba T; Masum MA; Tsolmon C; Yaser Hosny Ali ELEWA; Yasuhiro KON
  The 10th Sapporo summer symposium for one health (SaSSOH), 15 Sep. 2022
• Comparison of genital organ associated lymphoid tissue in vaginal vestibule of goat and pig
  Tsolmon CHULUUNBAATAR; Osamu ICHII; Takashi NAMBA; Md. Abdul MASUM; Yaser Hosny Ali ELEWA; Yasuhiro KON
  The 10th Sapporo summer symposium for one health (SaSSOH), 15 Sep. 2022
• The relation between systemic immunity and mucosal immunity of the head in autoimmune disease-prone BXSB/MpJ-Yaa mice
  Masaya HIRAISHI; Osamu ICHII; Teppei NAKAMURA; Yuki OTANI; Takashi NAMBA、Ali Elewa; Yaser Hosny; Yasuhiro KON
  The 10th Sapporo summer symposium for one health (SaSSOH), 14 Sep. 2022
• Pathological functions of collagen 17A1 in urothelial cells with obstructive uropathy
  Takashi NAMBA; Osamu ICHII; Ken NATSUGA; Teppei NAKAMURA; Yuki OTANI; Yasuhiro KON
  The 10th Sapporo summer symposium for one health (SaSSOH), 14 Sep. 2022
• 乳用種廃用牛を利用した移植用結合組織体の作製 －スリット形状が与える影響－
  片山由佳; 市居修; 柳瀬圭太; 中村鉄平; 池田哲平; 難波貴志; 大谷祐紀; 昆泰寛; 西邑隆徳
  第165回日本獣医学会学術集会, 07 Sep. 2022
• Genital organ associated lymphoid tissue comparison in vaginal vestibule of goat and pig
  Tsolmon Chuluunbaatar; 市居修; 難波貴志; Md. Abdul Masum; Yaser Hosny Ali Elewa; 昆泰寛
  第165回日本獣医学会学術集会, 07 Sep. 2022
• Lymphoid tissues found in mouse anorectal mucosa and systemic factors affecting their morphology
  Rubel MZU; Ichii O; Namba T; Masum MA; Tsolmon C; Yaser Hosny Ali ELEWA; Yasuhiro KON
  第165回日本獣医学会学術集会, 06 Sep. 2022
• コットンラットの系統差比較が解き明かすヒト下咽頭梨状窩瘻の由来
  中村鉄平; 細谷実里奈; 市居修; 渡邉敬文; Yaser Hosny Ali Elewa; 入江隆夫; 篠原明男; 昆泰寛
  第165回日本獣医学会学術集会, 06 Sep. 2022
• 尿管閉塞モデルマウスの尿管移行上皮に誘導される17型コラーゲンの発現動態
  難波貴志; 市居修; 中村鉄平; 大谷祐紀; 昆泰寛
  第165回日本獣医学会学術集会, 06 Sep. 2022
• BXSB/MpJ-Yaaマウスの病態からみる全身免疫と頭部粘膜免疫の関連
  平石真也; 市居修; 中村鉄平; 大谷祐紀; 難波貴志; Ali Elewa; Yaser Hosny; 昆泰寛
  第165回日本獣医学会学術集会, 06 Sep. 2022
• 変性コラーゲンのin situ多重染色―医療機器および腎線維化の評価への応用
  中村鉄平; 市居修; 片山由佳; 柳瀬圭太; 鷲見嘉奈子; 辻絵里香; 西邑隆徳; 三嶋隆; 森松正美
  第14回日本獣医腎泌尿器学会学術集会, 28 Aug. 2022
• 閉塞性尿路疾患において移行上皮細胞が発現する17型コラーゲンの多機能性
  難波貴志; 市居修; 夏賀健; 中村鉄平; 大谷祐紀; 昆泰寛
  第14回日本獣医腎泌尿器学会学術集会, 28 Aug. 2022
• ウシの皮下埋植基材周囲に形成される結合組織体の形態解析
  片山由佳; 市居修; 柳瀬圭太; 中村鉄平; 池田哲平; 難波貴志; 大谷祐紀; 昆泰寛; 西邑隆徳
  第2回獣医解剖アカデミア, 02 Apr. 2022
• エジンバラ大学解剖実習における動物利用
  大谷祐紀; 市居修; 昆泰寛
  第2回獣医解剖アカデミア, 02 Apr. 2022
• コットンラットが解き明かすヒト下咽頭梨状窩瘻の真の由来
  中村鉄平; 細谷実里奈; 市居修; 渡邉敬文; Yaser Hosny Ali Elewa; 入江隆夫; 篠原明男; 昆泰寛
  第2回獣医解剖アカデミア, 02 Apr. 2022
• Lymphoid tissues found in mouse anorectal mucosa and systemic factors affecting their morphology
  Rubel MZU; Ichii O; Namba T; Masum MA; Kon Y
  02 Apr. 2022
• 腎盤組織構造の部位差と動物種差 －尿路関連リンパ組織との関連－
  難波貴志; 市居修; 中村鉄平; 大谷祐紀; 昆泰寛
  第2回獣医解剖アカデミア, 02 Apr. 2022
• 尿のユニークな役割 －動物の腎臓病を知らせ、組織局所の免疫を変化させる体液－
  市居修
  第95回日本薬理学会年会, 07 Mar. 2022, Public symposium
  [Invited]
• ヒトH1N1インフルエンザAウイルスは発育鶏卵の羊膜・尿膜に発現するα2,3結合シアル酸を含む硫酸化糖鎖分子をレセプターとして利用する
  高瀬明; 一宮智美; 岡松正敏; 木下貴明; 小林大樹; 市居修; 山本直樹; 迫田義博; 喜田宏; 川島博人; 山本一夫; 西原祥子
  第68回日本ウイルス学会学術集会, Japanese, Oral presentation
  16 Nov. 2021 - 18 Nov. 2021
• ヒトH1インフルエンザAウイルスの発育鶏卵での増殖に寄与するレセプター構造の解明
  高瀬明; 一宮智美; 岡松正敏; 木下貴明; 小林大樹; 市居修; 山本直樹; 迫田義博; 喜田宏; 川島博人; 山本一夫; 西原祥子
  第40回日本糖質学会年会, Japanese, Oral presentation
  27 Oct. 2021 - 29 Oct. 2021
• ワクモ（Dermanyssus gallinae）由来システインプロテアーゼの抗ワクモワクチン抗原としての評価
  有泉 拓馬; 村田 史郎; 藤澤 宗太郎; 伊勢崎 政美; 佐藤 匠; 大石 英司; 種子野 章; 市居 修; 前川 直也; 岡川 朋弘; 今内 覚; 大橋 和彦
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• 骨軟骨組織に対する新規多重染色法(JFRL染色)の有用性
  中村 鉄平; 鷲見 嘉奈子; 辻 絵里香; 都築 奈津子; 近藤 大輔; 辻尾 祐志; 細谷 実里奈; 難波 貴志; 入江 隆夫; 市居 修; 昆 泰寛; 三嶋 隆
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• Genital Lymphoid Ring found in the vaginal vestibule of cow
  Chuluunbaatar Tsolmon; 市居 修; Masum Md. Abdul、Ali Elewa; Yaser Hosny; 昆 泰寛
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• Clarification of vasculature-associated lymphoid tissue in goat kidney
  Masum Md; Abdul; 市居 修; Ali Elewa; Yaser Hosny; 昆 泰寛
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• ネコの慢性腎臓病における腎臓組織の酸化ストレスと病態との関連性
  藏原 なつめ; 大和 修; 市居 修; 矢吹 映
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• マウス卵管線毛上皮細胞と排卵卵子の相互作用 －加齢と免疫異常は卵子輸送速度を変化させる－
  細谷 実里奈; 市居 修; 渡邉 敬文; 植田 弘美; 昆 泰寛
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• Ameliorative effect of dexamethasone on the development of autoimmune lung injury and mediastinal fat-associated lymphoid clusters in autoimmune disease mouse model
  Ali Elewa; Yaser Hosny; Masum; Md. Abdul; 市居 修; 中村 鉄平; Khairy Sherif; 昆 泰寛
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• シチメンチョウの気管上皮に発現する多様な鳥インフルエンザウイルスレセプターの構造解析
  小林 大樹; 市居 修; 日尾野 隆大; 西原 祥子; 高瀬 明; 磯田 典和; 迫田 義博
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• マウス全身免疫状態の違いによる異物応答の修飾
  山川 智大; 市居 修; 難波 貴志; 大谷 祐紀; 中村 鉄平; Masum Md. Abdul; Ali Elewa; Yaser Hosny; 昆 泰寛
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• 乳用種廃用牛を利用した移植用結合組織体の作製－経時的構成変化の解析－
  片山 由佳; 市居 修; 柳瀬 圭太; 中村 鉄平; 池田 哲平; 難波 貴志; 大谷 祐紀; 昆 泰寛; 西邑 隆徳
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• 慢性腎炎における尿と腎盤上皮の病態連関
  市居 修; 細谷 実里奈; Md. Abdul Masum; 堀野 太郎; 中村 鉄平; 難波 貴志; 大谷 祐紀; Yaser Hosny Ali Elewa; 昆 泰寛
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• Interleukin-36受容体を欠損した自己免疫性腎炎モデルマウスの腎表現型解析
  難波 貴志; 市居 修; 岡村 匡史; 中野 堅太; 中村 鉄平; 大谷 祐紀; Masum Md. Abdul; Ali Elewa; Yaser Hosny; 昆 泰寛
  第164回日本獣医学会学術集会, Japanese, Oral presentation
  07 Sep. 2021 - 13 Sep. 2021
• イヌとネコの腎臓におけるSGLT2の発現
  市居修; 大谷祐紀; Masum Md.Abdul; 難波貴志; Yaser Elewa; 昆泰寛
  第13回 日本獣医腎泌尿器学会 学術集会・総会, Japanese, Oral presentation
  14 Aug. 2021 - 30 Aug. 2021
• 下部消化管の腫瘍外科「解剖学」
  市居 修
  第102回 日本獣医麻酔外科学会 オンライン学術集会, 29 Aug. 2021, Japanese, Nominated symposium
  [Invited]
• シチメンチョウの気管上皮に発現する多様な鳥インフルエンザウイルスレセプターの分布と構造の解析
  小林 大樹; 市居 修; 日尾野 隆大; 磯田 典和; 高瀬 明; 西原 祥子; 山本 一夫; 迫田 義博
  第54回日本ウイルス学会北海道支部会夏季シンポジウム, Japanese, Oral presentation
  31 Jul. 2021 - 01 Aug. 2021
• ヒトH1亜型インフルエンザAウイルスの卵馴化に寄与するレセプター構造の解明
  高瀬 明; 一宮智美; 岡松正敏; 木下貴明; 小林大樹; 市居修; 山本直樹; 迫田義博; 喜田宏; 川島博人; 山本一夫; 西原祥子
  第34回インフルエンザ研究者交流の会, Japanese, Oral presentation
  01 Jul. 2021 - 02 Jul. 2021
• 汎動物学からみた⾝近な動物の腎臓とその病態
  市居 修
  第64回日本腎臓学会学術総会, 20 Jun. 2021, Japanese, Nominated symposium
  [Invited]
• Genital lymphoid ring found in vulva/vaginal vestibule of animals
  Tsolmon Chuluunbaatar; Osamu Ichii; Md Abdul Masum; Yaser Hosny Ali Elewa; Yasuhiro Kon
  第1回 ⽇本獣医解剖アカデミア, 06 Mar. 2021, Japanese, Oral presentation
• Comperison of ovarian morphology and follicular disturbancesbetween two inbred strains ofl cotton rat (Sigmodon hispidus)
  Md Rashedul Islam; Osamu Ichii; Teppei Nakamura; Takao Irie; Akio Shinohara; Md abdul masum; Yaser Hosny Ali Elewa; Yasuhiro Kon
  第1回 ⽇本獣医解剖アカデミア, 06 Mar. 2021, Japanese, Oral presentation
• 加齢の Interleukin 36 受容体欠損マウスで観察される上皮組織の形態異常
  難波貴志; 市居修; 中村鉄平; 大谷祐紀; Masum Md. Abdul; Yaser Hosny Ali Elewa; 昆泰寛
  第1回 ⽇本獣医解剖アカデミア, 06 Mar. 2021, Japanese, Oral presentation
• イヌの外頭蓋底にみられる⾆下神経管と顆管の個体差
  大谷祐紀; 市居 修; 難波貴志; 昆 泰寛
  第1回 ⽇本獣医解剖アカデミア, 06 Mar. 2021, Japanese, Oral presentation
• 北海道大学の獣医解剖学教育における感染症対策－2020 年度－
  市居 修; 難波貴志; 大谷祐紀; Yaser Hosny Ali Elewa; 昆 泰寛
  第1回 ⽇本獣医解剖アカデミア, 06 Mar. 2021, Japanese
• Elucidation of vasculature-associated tertiary lymphoid tissue in lupus nephritis mouse model
  Md. Abdul Masum; 市居 修; 難波貴志; 大谷祐紀; Yaser Hosny Ali Elewa; 昆 泰寛
  第1回 ⽇本獣医解剖アカデミア, 06 Mar. 2021, Japanese, Oral presentation
• Functional attribution of mediastinal fat-associated lymphoid clusters’ innate immune cells in papain induced lung asthma mouse model
  Y. H. A. Elewa; O. Ichii; T. Nakamura; Y. Kon
  第1回 ⽇本獣医解剖アカデミア, 06 Mar. 2021, English, Oral presentation
• 会陰ヘルニアに関連する局所解剖学
  市居 修
  日本獣医麻酔外科学会 北海道地区オンライン講習会, 14 Feb. 2021, Japanese, Public discourse
  [Invited]
• コットンラットの尾の組織学的特徴および自切機序の解析
  中村 鉄平; 細谷 実里奈; 市居 修; 入江 隆夫; 寸田 祐嗣; Yaser Hosny Ali Elewa; 三嶋 隆; 渡邉 敬文; 植田 弘美; 昆 泰寛
  第163回日本獣医学会学術集会, Japanese, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• ネコ尿管の形態と尿管結石症による変化
  市居 修; 小山田 和央; 水川 葉月; 中村 鉄平; Ali Elewa; Yaser Hosny; 昆 泰寛
  第163回日本獣医学会学術集会, Japanese, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• 自己免疫疾患モデルマウスの解析からみる腎臓内IL-36群の機能的棲み分け
  難波 貴志; 市居 修; 中村 鉄平; 大谷 祐紀; Md. Abdul Masum; Yaser Hosny Ali Elewa; 昆 泰寛
  第163回日本獣医学会学術集会, Japanese, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• アンドロゲンは自己免疫性腎炎モデルマウスの糸球体硬化を増悪させる
  大谷 祐紀; 市居 修; Md. Abdul Masum; 難波 貴志; 昆 泰寛
  第163回日本獣医学会学術集会, Japanese, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• 自己免疫疾患モデルマウスを用いた糖尿病性腎臓病の病態解明アプローチ
  平松 栞; 市居 修; 難波 貴志; 大谷 祐紀; Md. Abdul Masum; 中村 鉄平; Yaser Hosny Ali Elewa; 昆 泰寛
  第163回日本獣医学会学術集会, Japanese, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• Podocyte injury via Toll-like receptor 8 signaling in experimental obstructive nephropathy in mice
  Md. Abdul Masum; 市居 修; Yaser Hosny Ali Elewa; 昆 泰寛
  第163回日本獣医学会学術集会, English, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• Developmental changes of the ovary in the neonatal cotton rat（Sigmodon hispidus）
  Md. Rashedul Islam; 市居 修; 中村 鉄平; 入江 隆夫; Md. Abdul Masum; Yaser Hosny Ali Elewa; 昆 泰寛
  第163回日本獣医学会学術集会, Japanese, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• マウス卵管の線毛におけるヘッジホッグシグナル伝達経路の発現
  細谷 実里奈; 市居 修; 中村 鉄平; 長谷川 靖洋; Md. Abdul Masum; 渡邉 敬文; 植田 弘美; 昆 泰寛
  第163回日本獣医学会学術集会, Japanese, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• 鳥インフルエンザウイルスの中間宿主としてのウズラおよびシチメンチョウにおけるシアル酸糖鎖分布の解析
  小林 大樹; 佐々木 乙; 日尾野 隆大; 岡松 正敏; 西原 祥子; 高瀬 明; 市居 修; 松野 啓太; 迫田 義博
  第163回日本獣医学会学術集会, Japanese, Oral presentation
  14 Sep. 2020 - 30 Sep. 2020
• 腎泌尿器の発生、解剖および形態異常
  市居 修
  日本獣医腎泌尿器学会 2020年度 認定講習会, 27 Sep. 2020, Japanese, Public discourse
• 導入とHistoJAVAの進捗状況
  市居 修
  日本獣医解剖学会 第12回KEMONO会, 23 Sep. 2020, Japanese, Oral presentation
• Species specific differences of large intestinal pattern and its unique mucosal morphology in the Cotton rats
  Tsolmon Chuluunbaatar; Osamu Ichii; Teppei Nakamura; Takao Irie; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 8th Sapporo Summer Seminar for One Health (SaSSOH), English, Oral presentation
  16 Sep. 2020 - 17 Sep. 2020
• Developmental changes of the ovary in the neonatal cotton rat (Sigmodon hispidus)
  Md Rashedul Islam; Osamu Ichii; Teppei Nakamura; Takao Irie; Md abdul masum; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 8th Sapporo Summer Seminar for One Health (SaSSOH), English, Oral presentation
  16 Sep. 2020 - 17 Sep. 2020
• Enhanced IL-36α expression in the kidneys of autoimmune disease-prone mice
  Takashi NAMBA; Osamu ICHII; Teppei NAKAMURA; Md. Abdul MASUM; Yaser Hosny Ali ELEWA; Yasuhiro KON
  The 8th Sapporo Summer Seminar for One Health (SaSSOH), English, Oral presentation
  16 Sep. 2020 - 17 Sep. 2020
• Orchidectomized autoimmune disease model mouse shows ameliorated sclerotic lesion in the glomerulus
  OTANI Yuki; ICHII Osamu; KON Yasuhiro
  The 8th Sapporo Summer Seminar for One Health (SaSSOH), English, Poster presentation
  16 Sep. 2020 - 17 Sep. 2020
• Pathological alternations of mediastinal fat-associated lymphoid cluster in streptozotocin- induced diabetic mouse model
  Y. H. A. Elewa; O. Ichii; T. Nakamura; Y. Kon
  日本解剖学会 第65回東北・北海道連合支部学術集会, English, Oral presentation
  05 Sep. 2020 - 06 Sep. 2020
• Smart Metabolite Database を用いた BDE209 長期曝露試験によるネコ血清中メタボロームの変動解析
  野見山 桂; 林 多聞; 江口 哲史; 水川 葉月; 髙口 倖暉; 横山 望; 市居 修; 滝口 満喜; 笹岡 一慶; 中山 翔太; 池中 良徳; 石塚 真由美; 国末 達也
  第29回環境化学討論会, 02 Jun. 2020, Japanese, Oral presentation
• 走査型顕微鏡の応用的手法による腎病理評価
  市居 修; Md. Abdul Masum; Yaser Hosny Ali Elewa; 昆 泰寛
  日本顕微鏡学会第62回シンポジウム, 29 Nov. 2019
• Possible role of the novel mediastinal fat associated lymphoid clusters in human, animals and poultry respiratory diseases progression
  Y. H. A. Elewa; O. Ichii; T. Nakamura; Y. Kon. The; international scientific conference; faculty of veterinary medicine
  The 4th international scientific conference, 10 Oct. 2019
• 自己免疫疾患モデルマウスにみられる涙液産生組織の形態学的変化
  平石 真也; 市居 修; 中村 鉄平; 難波 貴志; Yaser Hosny Ali Elewa; 昆 泰寛
  第162回日本獣医学会学術集会, 12 Sep. 2019
• コットンラットの下咽頭梨状窩瘻を構成する細胞の組織化学的特徴
  中村 鉄平; 市居 修; 入江 隆夫; 寸田 祐嗣; 細谷 実里奈; 長崎 健一; Yaser Hosny Ali Elewa; 三嶋 隆; 昆 泰寛
  第162回日本獣医学会学術集会, 12 Sep. 2019
• 自己免疫疾患モデルマウスで変化する腎臓内 Interleukin 1 family の発現バランス
  難波 貴志; 市居 修; 中村 鉄平; Yaser Hosny Ali Elewa; 昆 泰寛
  第162回日本獣医学会学術集会, 12 Sep. 2019
• 動物の尿路関連リンパ組織（UTALT）に発現する分子群
  市居 修; 中村 鉄平; 堀野 太郎; 細谷 実里奈; Yaser Hosny Ali Elewa; 昆 泰寛
  第162回日本獣医学会学術集会, 12 Sep. 2019
• 加齢マウスの尿生殖器における局所リンパ組織の探索
  山下 渚; 市居 修; 大谷 祐紀; Masum Md.Abdul; Yaser Hosny Ali Elewa; 昆 泰寛
  第162回日本獣医学会学術集会, 12 Sep. 2019
• マウス生体内異物反応の経時観察 ―組織局所の炎症と線維化の関連性―
  大江 紗央; 市居 修; 難波 貴志; 大谷 祐紀; Masum Md. Abdul; 中村 鉄平; Yaser Hosny Ali Elewa; 西邑 隆徳; 中山 泰秀; 昆 泰寛
  第162回日本獣医学会学術集会, 12 Sep. 2019
• マウスの卵巣嚢－腹腔連絡路（卵巣嚢孔）
  細谷 実里奈; 市居 修; 中村 鉄平; 難波 貴志; Yaser Hosny Ali Elewa; 渡邉 敬文; 植田 弘美; 昆 泰寛
  第162回日本獣医学会学術集会, 12 Sep. 2019
• Unique morphological characteristics in the ovary of cotton rat（Sigmodon hispidus）
  Md Rashedul Islam; 市居 修; 中村 鉄平; 入江 隆夫; Masum Md Abdul; Yaser Hosny Ali Elewa; 昆 泰寛
  第162回日本獣医学会学術集会, 12 Sep. 2019
• ワクモ由来Adipocyte plasma membrane-associated proteinの抗ワクモワクチン抗原としての評価
  森田 鮎; 竹原 昌生; 村田 史郎; 伊勢崎 政美; 藤澤 宗太郎; 種子野 章; 酒井 英史; 宇野 有紀子; 小川 遼; 市居 修; 前川 直也; 岡川 朋弘; 今内 覚; 大橋 和彦
  第162回日本獣医学会学術集会, 12 Sep. 2019
• 慢性腎臓病の猫の腎臓におけるperoxisome proliferator activated receptor-γの発現動態
  矢吹 映; 上原 由季; 吉田 千紘; 市居 修; 大和 修
  第162回日本獣医学会学術集会, 10 Sep. 2019
• 尿路関連リンパ組織と17型コラーゲンの発現
  市居修; 中村鉄平; 堀野太郎; 細谷実里奈; Yaser Hosny Ali Elewa; 昆泰寛
  第65回東北・北海道連合支部学術集会, 07 Sep. 2019
• Possible role of natural helper cells within the mediastinal fat-associated lymphoid cluster and lung in papain induced lung asthma mouse model
  Yaser Hosny Ali Elewa; 市居修; 中村鉄平; 昆泰寛
  第65回東北・北海道連合支部学術集会, 07 Sep. 2019
• SLC:Wistar/STラットで認めた片側性甲状腺形成不全の解析
  中村鉄平; 市居修; 寸田祐嗣; Yaser Hosny Ali Elewa; 服部秀樹; 長﨑健一; 昆泰寛
  第65回東北・北海道連合支部学術集会, 07 Sep. 2019
• Effect of systemic autoimmune disease on the mouse reproductive function.
  uki Otani; Osamu Ichii; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 7th Congress of Asian Association of Veterinary Anatomists (Asian AVA)
  Sep. 2019
• Elucidation of the pathogenesis of lung asthma development and recovery: Possible immune cells’ crosstalk within lung and mediastinal fat associated lymphoid clusters in papain induced lung asthma mice model.
  Yaser Hosny Ali Elewa; Osamu, Ichii; Teppei, Nakamura; Yasuhiro, Kon
  The 7th Congress of Asian Association of Veterinary Anatomists (Asian AVA)., Sep. 2019
• Application of virtual slide system to histological education in Hokkaido University.
  Osamu Ichii; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 7th Congress of Asian Association of Veterinary Anatomists (Asian AVA)., Sep. 2019
• Unique morphological characteristics in the ovary of cotton rat (Sigmodon hispidus).
  Md. Rashedul Islam; Osamu Ichii; Teppei Nakamura; Takao Irie; Md. Abdul Masum; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 7th Congress of Asian Association of Veterinary Anatomists (Asian AVA)., Sep. 2019
• Altered bone morpho-function in autoimmune disease-prone mice
  Takashi Namba; Osamu Ichii; Md. Abdul Masum; Yuki Otani; Saori Otsuka-Kanazawa; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 7th Congress of Asian Association of Veterinary Anatomists (Asian AVA), Sep. 2019
• 動物の尿生殖器関連リンパ組織
  市居 修; 中村 鉄平; 堀野 太郎; 山下 渚; 細谷 実里奈; Yaser Hosny Ali Elewa; 昆 泰寛
  第12回 日本獣医腎泌尿器学会学術集会, Aug. 2019
• 犬におけるシスプラチン投与による腎傷害と臨床病理学的パラメータの変動
  岡本茉里; 市居 修; 三重慧一郎; 西田英高; 秋吉秀保
  第12回 日本獣医腎泌尿器学会学術集会, Aug. 2019
• PCBs 曝露によるネコ肝トランスクリプトームへの影響 Effects of PCBs exposure to the feline liver transcriptome
  水川 葉月; Hoa Thanh Ngyuen; 岩田久人; 野見山 桂; 池中良徳; 中山翔太; 横山 望; 市居 修; 滝口満喜; Kraisiri Khidkhan; 田辺信介; 石塚真由美
  第29回環境化学討論会, 24 Jun. 2019
• デカブロモジフェニルエーテル（BDE209）の長期曝露がイエネコへ及ぼす影響 In vivo toxicokinetic analysis of long-term exposure to BDE209 on domestic cat
  田中 啓介; 野見山 桂; 水川 葉月; 髙口 倖暉; 田上 瑠美; 横山 望; 市居 修; 滝口 満喜; 笹岡 一慶; 中山 翔太; 池中 良徳; 石塚 真由美; 国末 達也; 田辺 信介
  第29回環境化学討論会, 24 Jun. 2019
• iBTA －汎動物学への応用－
  市居 修
  第一回iBTA再生医療研究会, 02 Mar. 2019
• 蛋白尿―糸球体の形態機能とその制御分子から―
  市居修
  第15回日本獣医内科学アカデミー, 17 Feb. 2019
• Innate immunity through Mediastinal fat-associated lymphoid cluster in papain induced lung asthma mice model.
  Yaser Hosny Ali Elewa; Osamu, Ichii; Teppei, Nakamura; Yasuhiro, Kon
  The 42nd Annual Conference of the Egyptian Society of Histology and Cytology., Dec. 2018
• Effects of PCB exposure on thyroid hormone levels in serum of dogs and cats
  Kohki Takaguchi; Hiroyuki Nishikawa; Hazuki Mizukawa; Rumi Tanoue; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Shouta M; M. Nakayama; Yoshinori Ikenaka; Tatsuya Kunisue; Mayumi Ishizuka; Shinsuke Tanabe; Hisato Iwata; d; Kei
  SETAC North America 39th Annual Meeting, 04 Nov. 2018
• iBTA組織のoff-the-shelf人工臓器をめざして：ウシバイオシートの異種移植
  中山泰秀; 大島奈公子; 古越真耶; 巽 英介; 市居 修; 西邑 隆徳
  第56回日本人工臓器学会大会, 02 Nov. 2018
• 生体内組織形成術(iBTA)で生産したウシ由来バイオシートの組織構造と移植適性
  今山 知佳; 鈴木 貴弘; 小林 謙; 三谷 朋弘; 市居 修; 中山 泰秀; 古越 真耶; 池田 哲平; 小千田 圭吾; 西邑 隆徳
  第14回結合組織勉強会, 27 Oct. 2018
• Autoimmune disease alters systemic ciliary function in MRL/MpJ-Faslpr/lpr mice
  細谷実里奈; 市居 修; Yaser Hosny Ali Elewa; 中村鉄平; 昆 泰寛
  The 6th Sapporo Summer Symposium for One Health, 20 Sep. 2018
• Causative molecules to increase the apoptosis of meiotic spermatocytes in autoimmune disease-prone BXSB/MpJ-Yaa mice
  大谷祐紀; 市居 修; Yaser Hosny Ali Elewa; 昆 泰寛
  The 6th Sapporo Summer Symposium for One Health, 20 Sep. 2018
• Overexpression of Toll-like receptor 9 correlates with podocyte injury in murine autoimmune glomerulonephritis
  Md. Abdul Masum; 市居 修; Yaser Hosny Ali Elewa; 中村 鉄平; 大谷 祐紀; 細谷 実里奈; 昆 泰寛
  The 6th Sapporo Summer Symposium for One Health, 20 Sep. 2018
• ヒトコブラクダ甲状腺の形態学的特徴－鰓後体遺残とC細胞の分布－
  中村鉄平; 市居修; Yaser Hosny Ali Elewa; 細谷実里奈; Wael A. M. Ghonimi; 辰巳治; 長崎健一; 昆泰寛
  第161回日本獣医学会学術集会, 11 Sep. 2018
• 自己免疫疾患モデルマウスの骨に観察される形態機能的変化
  難波貴志; 市居 修; Yaser Hosny Ali Elewa; 中村鉄平; 木村純平; 大谷祐紀; Md. Abdul Masum; 昆 泰寛
  第161回日本獣医学会学術集会, 11 Sep. 2018
• 腎疾患の新知見－Casp3欠損マウスにみられる腎病変－
  鈴木崇史; 市居修; 中村鉄平; Yaser Hosny Ali Elewa; 昆泰寛
  第161回日本獣医学会学術集会, 11 Sep. 2018
• MRL/MpJ-Faslpr/lprマウスの全身自己免疫異常が線毛運動機能に与える影響
  細谷 実里奈; 市居 修; Yaser Hosny Ali Elewa; 中村 鉄平; 昆 泰寛
  第161回日本獣医学会学術集会, 11 Sep. 2018
• 自己免疫疾患モデルマウスで増加する減数分裂特異的精母細胞アポトーシスの原因探索
  大谷祐紀; 市居 修; Yaser Hosny Ali Elewa; 木村純平; Md. Abdul Masum; 中村鉄平; 昆 泰寛
  第161回日本獣医学会学術集会, 11 Sep. 2018
• 新たな腎病態マーカー分子の発見と臨床獣医学への応用に関する研究
  市居 修
  第161回日本獣医学会学術集会, 11 Sep. 2018
• 動物の尿路に出現するリンパ組織（Urinary tract-associated lymphoid tissue, UTALT）
  市居 修; 中村 鉄平; 細谷 実里奈; 堀野 太郎; Yaser Hosny Ali Elewa; 昆 泰寛
  第161回日本獣医学会学術集会, 11 Sep. 2018
• Overexpression of Toll-like receptor 9 correlates with podocyte injury found in murine autoimmune glomerulonephritis
  Md. Abdul Masum; 市居 修; Yaser Hosny Ali Elewa; 中村 鉄平; 大谷 祐紀; 細谷 実里奈; 昆 泰寛
  第161回日本獣医学会学術集会, 11 Sep. 2018
• ウシの眼関連リンパ組織について
  小千田 圭吾; 市居 修; 中村 鉄平; 池田 哲平; 山下 祐輔; 長澤 裕哉; Yaser Hosny Ali Elewa; 昆 泰寛
  第161回日本獣医学会学術集会, 11 Sep. 2018
• Mediastinal fat-associated lymphoid cluster and lung asthma development in papain induced lung asthma mice model
  Y. H. A. Elewa; T. Nakamura; O. Ichii; Y. Kon
  第64回東北・北海道連合支部学術集会, 01 Sep. 2018
• The estimation of potential PCBs toxicity in cats by evaluating CYP450 expressions
  KHIDKHAN Kraisiri; Hazuki Mizukawa; Yoshinori Ikenaka; Shouta M; M. Nakayama; Kei Nomiyama; Mitsuyoshi Takiguchi; Nozomu Yokoyama; Osamu Ichii; Shinsuke Tanabe; Mayumi Ishizuka
  Dioxin 2018 : The 38th International Symposium on Halogenated Persistent Organic Pollutants & 10th International PCB Workshop, 25 Aug. 2018
• 雄性コットンラット(Sigmodon hispidus)にみられる糸球体病変
  中村鉄平; 市居 修; 入江隆夫; 孝口裕一; 寸田祐嗣; 堀野太郎; Md. Abdul Masum; 辰巳治; Yaser Hosny Ali Elewa; 昆 泰寛
  第11回 日本獣医腎泌尿器学会学術集会, 19 Aug. 2018
• ネコの尿中exosomeに含まれるマイクロRNAの解析
  市居 修; 大田 寛; 堀野太郎; 中村鉄平; 細谷実里奈; 溝口達也; 森下啓太郎; 中村健介; 佐々木 東; 滝口満喜; 佐藤 遼; 小山田和央; 昆泰寛
  第11回 日本獣医腎泌尿器学会学術集会, Aug. 2018
• 有機ハロゲン化合物曝露によるネコの生 化学マーカーの変化および甲状腺機能に及ぼす影響評価
  田中 啓介; 野見山 桂; 水川 葉月; 高口 倖暉; 田上 瑠美; 横山 望; 市居 修; 滝口 満喜; 中山 翔太; 池中 良徳; 石塚真由美; 国末達也; 田辺 信介
  第27回環境化学討論会, May 2018
• ネコの in vivo BDE209 長期曝露試験による体内動態の解明および甲状腺ホルモンへの影響評価
  田中 啓介; 野見山 桂; 水川 葉月; 高口 倖暉; 田上 瑠美; 横山 望; 市居 修; 滝口 満喜; 中山 翔太; 池中 良徳; 石塚真由美; 国末達也; 田辺 信介
  第27回環境化学討論会, May 2018
• Local CD34-positive capillary decreased with the progression of lesion in respective area of kidney in mice.
  Md. A. Masum; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon
  The 17th International Conference on Nephrology and Urology, 13 Mar. 2018
• Dual effects of bleomycin on the intra-thoracic immune hemostasis and lung injury in autoimmune disease model mice
  Y. H. A. Elewa; O. Ichii, S; Y. Kon
  The 41st Annual International Conference of Medical Histology & Cell Biology, Regenerative Medicine & Cell Transplantation., Dec. 2017
• 環境ホルモン学会第20回研究発表会 ポリ塩化ビフェニル（PCBs）がネコの甲状腺機能に及ぼす影響
  水川葉月; 高居名菜; 野見山桂; 高口倖暉; 横山望; 市居修; 滝口満喜; 池中良徳; 中山翔太; 石塚真由美
  環境ホルモン学会第20回研究発表会, Dec. 2017
• Discovery of renal biomarkers in birds by transcriptome analysis and glycomics analysis following kidney injury in chicken model.
  Ishii C; Ikenaka Y; Kawai KY; Ichii O; Nakayama SMM; Mizukawa H; Ishizuka M
  SETAC North America 38th Annual Meeting, Nov. 2017
• ABNORMALITY OF OVULATION AND OOCYTE-PICK-UP IN MRL/MpJ-Faslpr/lpr MICE.
  Marina Hosotani; Osamu Ichii; Yaser Hosny Ali Elewa; Teppei Nakamura; Saori Otsuka-Kanazawa; Yasuhiro Kon
  The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA)., Oct. 2017
• BXSB/MpJ-Yaa MICE SHOW THE INCREASE OF APOPTOTIC SPERMATOCYTES IN STAGES Ⅻ SEMINIFEROUS TUBULES.
  Yuki Otani; Osamu Ichii; Yaser Hosny Ali Elewa; Junpei Kimura; Md. Abdul Masum; Teppei Nakamura; Yasuhiro Kon
  The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA)., Oct. 2017
• IL-1F6/IL-36α SIGNALING REGULATES RENAL INFLAMMATION.
  Osamu Ichii; Junpei Kimura; Tadashi Okamura; Taro Horino; Teppei Nakamura; Hayato Sasaki; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA)., Oct. 2017
• DUAL EFFECTS OF BLEOMYCIN ON THE INTRA-THORACIC IMMUNE HEMOSTASIS AND LUNG INJURY IN AUTOIMMUNE DISEASE MODEL MICE.
  Yaser Hosny Ali Elewa; Osamu Ichii; Teppei Nakamura; Yasuhiro Kon
  The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA), Oct. 2017
• Short-term or long-term in vivo exposure to organohalogen compounds in cats: Elucidate the metabolic capacities and effects on thyroid function
  Hazuki Mizukawa; Yoshinori Ikenaka; Shouta M.M. Nakayama; Kei Nomiyama; Misaki Maehara; Aksorn Saengtienchai; Tsend-ayush Sainnoxoi; Kraisiri Khidkhan; Nozomu Yokoyama; Kazuyoshi Sasaoka; Osamu Ichii; Mitsuyoshi Takiguchi; Khoki Takaguchi; Hiroki Nishikawa; Nana Takai; Shinsuke Tanabe; Mayumi Ishizuka
  9th International Toxicology Symposium in Nigeria, Sep. 2017
• Discovery of renal biomarker in birds by transcriptome analysis of kidney tissues and glycomics analysis of plasma following renal damage in chicken model.
  Ishii C; Ikenaka Y; Kawai KY; Ichii O; Nakayama, SMM; Mizukawa H; Ishizuka M
  The 5th Sapporo Summer Seminar for One Health, Sep. 2017
• 鳥類医学の発展に向けた新規腎障害マーカーの探索
  石井千尋; 池中良徳; 川合佑典; 市居修; 中山翔太; 西村紳一郎; 大橋哲; 田中誠一; 齊藤慶輔; 渡邊有希子; 水川葉月; 石塚真由美
  日本鳥学会2017年度大会, Sep. 2017
• 黄色ブドウ球菌死菌を抗原とした鼻腔粘膜感作による乳房炎防除効果
  長澤裕哉; 菊佳男; 菅原和恵; 甲斐千暁; 澤田晋一; 秋吉一成; 林智人
  第160回日本獣医学会学術集会, Sep. 2017
• Sarcocystis属原虫の終宿主候補としてのキタキツネにおける調査
  入江隆夫; 浦口宏二; 中村鉄平; 市居 修; 髙井伸二; 八木欣平
  Sep. 2017
• Pathological correlations between endothelial cells and podocytes in autoimmune-related injury of the mouse glomerulus
  Md. Abdul Masum; 市居 修; Yaser Hosny Ali Elewa; 中村 鉄平; 昆 泰寛
  第160回日本獣医学会, Sep. 2017
• BXSB/MpJ-Yaaマウスにみられる精母細胞のアポトーシス－自己免疫異常との関連性－
  大谷 祐紀; 市居 修; Yaser Hosny Ali Elewa; 木村 純平; Md. Abdul Masum; 中村 鉄平; 昆 泰寛
  第160回日本獣医学会, Sep. 2017
• MRL/MpJマウスの排卵―黄体形成過程にみられる特異な表現型
  細谷 実里奈; 市居 修; Yaser Hosny Ali Elewa; 中村 鉄平; 昆 泰寛
  第160回日本獣医学会, Sep. 2017
• Dual effects of bleomycin on the intra-thoracic immune hemostasis and lung injury in autoimmune disease model mice.
  Y. H. A. Elewa; T. Nakamura; O. Ichii; Y. Kon
  The 63th annual meeting of the Japanese the Anatomical Society., Sep. 2017
• Histopathological features observed in the testis of autoimmune-prone BXSB/MpJ-Yaa mice.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon
  The 5th Sapporo Summer Seminar for One Health (SaSSOH), Sep. 2017
• Loss of endothelial fenestration correlates with podocyte injury in the mouse model of membranoproliferative glomerulonephritis.
  Md. A. Masum; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon. T
  The 5th Sapporo Summer Seminar for One Health (SaSSOH), Sep. 2017
• Mechanism and abnormality of ovulation and picking up of mammalian oocytes-Immune factors affecting the molecular morphology of the oviduct
  M. Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon
  The 5th Sapporo Summer Seminar for One Health (SaSSOH), Sep. 2017
• Effects of Organohalogen Compounds on Serum Biochemical Profiles in Cats Kraisiri Khidkhan
  Hazuki Mizukawa; Kei Nomiyama; Yoshinori Ikenaka; Shota M.M. Nakayama; Nozomu Yokoyama; Kazuyoshi Sasaoka; Osamu Ichii; Mitsuyoshi Takiguchi; Hiroyuki Nishikawa; Keisuke Tanaka; Kohki Takiguchi; Shinsuke Tanabe; Mayumi Ishizuka
  The 5th Sapporo Summer Seminar for One Health (SaSSOH), Sep. 2017
• In vivo exposure to PCBs in cats: Analysis of metabolic capacities and effects on the thyroid hormone homeostasis
  Hazuki Mizukawa; Kei Nomiyama; Hiroyuki Nishikawa; Misaki Maehara; Nana Takai; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Yoshinori Ikenaka; Shouta M.M. Nakayama; Kohki Takaguchi; Shinsuke Tanabe; Mayumi Ishizuka
  37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017), Aug. 2017
• 雌性コットンラット(Sigmodon hispidus)にみられる慢性腎臓病－子宮蓄膿症及び性ホルモンの関与－
  中村鉄平; 市居 修; 入江隆夫; 孝口裕一; 寸田祐嗣; 堀野太郎; 辰巳治; Yaser Hosny Ali Elewa; 昆 泰寛
  第10回日本獣医腎泌尿器学会, Aug. 2017
• 尿細管間質病変に関与するIL-36シグナリング
  市居 修; 木村 純平; 岡村 匡史; 堀野 太郎; 中村 鉄平; 矢吹 映; 佐々木 隼人; Yaser Hosny Ali Elewa; 昆 泰寛
  第10回日本獣医腎泌尿器学会, Aug. 2017
• ネコの甲状腺機能に対するポリ塩化ビフェニル（PCBs）の影響評価〜甲状腺ホルモンの脱ヨード化と代謝能の解析
  水川葉月; 高居名菜; 横山望; 市居修; 滝口満喜; 野見山桂; 高口倖暉; 西川博之; 池中良徳; 中山翔太; Tsend-ayush Sainnoxoi; 田辺信介; 石塚真由美
  第44回日本毒性学会, Jul. 2017, Japanese, Oral presentation
  [Domestic Conference]
• イエネコのin vivo長期曝露試験：デカブロモジフェニルエーテ (BDE209)の生体内変化
  田中啓介; 野見山桂; 水川葉月; 高口倖暉; 田上瑠美; Aksorn Saengtienchai,Tsend-ayush Sainnoxoi; 横山望; 市居 修; 滝口満喜; 中山翔太; 池中良徳; 石塚真由美; 国末達也; 田辺信介
  第26回環境化学討論会, Jun. 2017, Japanese, Oral presentation
  [Domestic Conference]
• PCBs曝露 がイヌ・ネコの甲状腺ホルモン恒常性へ及ぼす影響
  高口 倖暉; 野見山 桂; 西川 博之; 水川 葉月; 田上 瑠美; 芳之内 結加; 横山 望; 市居 修; 滝口満喜; 中山 翔太; 池中良徳; 石塚真由美; 岩田久人; 国末達也; 田辺信介
  第26回環境化学討論会, Jun. 2017, Japanese, Oral presentation
  [Domestic Conference]
• 血清中メタボローム解析によるイエネコのPCBs毒性影響評価
  野見山桂; 西川 博之; 水川葉月; 高口倖暉; Tsend-ayush Sainnoxoi; 横山 望; 市居 修; 滝口満喜; 石塚真由美; 池中良徳; 中山翔太; 江口哲史; 国末達也; 田辺信介
  第26回環境化学討論会, Jun. 2017, Japanese, Oral presentation
  [Domestic Conference]
• ポリ塩化ビフェニル（PCBs）暴露によるネコ甲状腺機能への影響評価 〜 甲状腺ホルモンの脱ヨード化と代謝能解析〜
  水川葉月; 高居名菜; 横山望; 市居修; 滝口満喜; 野見山桂; 高口倖暉; 西川博之; 池中良徳; 中山翔太; Tsend-ayush Sainnoxoi; 田辺信介; 石塚真由美
  第26回環境化学討論会, Jun. 2017, Japanese, Oral presentation
  [Domestic Conference]
• ウシ生体内で形成させた移植医療用組織の構造
  今山知佳; 鈴木貴弘; 小林 謙; 三谷朋弘; 市居 修; 小千田圭吾; 池田哲平; 寺澤 武; 中山泰秀
  日本畜産学会第122回大会, Mar. 2017, Japanese, Oral presentation
  [Domestic Conference]
• 家畜生体内を利用して再生移植医療用組織をつくる
  西邑隆徳; 今山知佳; 鈴木貴弘; 小林 謙; 三谷朋弘; 市居 修; 小千田圭吾; 池田哲平; 寺澤 武; 中山泰秀
  日本畜産学会第122回大会, Mar. 2017, Japanese, Oral presentation
  [Domestic Conference]
• RNAから展開する腎臓病研究 〜 獣医臨床へのアプローチ〜
  市居 修
  第13回日本獣医内科学アカデミー 学術大会, Feb. 2017, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• 腎臓病を知らせる microRNA −病態マーカーとしての可能性と問題点−
  市居 修
  第33回日本毒性病理学会学術集会, Jan. 2017, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• 生体内組織形成術による管状組織体の壁厚制御
  寺澤 武; 西邑 隆徳; 三谷 朋弘; 市居 修; 中山 泰秀
  第54回日本人工臓器学会大会, Nov. 2016, Japanese, Oral presentation
  [Domestic Conference]
• Characteristic for biotransformation of polychrolinated biphenyls in cats
  Hazuki Mizukawa; Misaki Maehara; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Kei Nomiyama; Hiroyuki Nishikawa; Yoshinori Ikenaka; Shouta M.M. Nakayama; Kohki Takaguchi; Shinsuke Tanabe; Mayumi Ishizuka
  The 7th SETAC World Congress/37th SETAC North America Annual Meeting, Nov. 2016, English, Oral presentation
  [International presentation]
• ウシの咽頭扁桃は鼻腔から投与した黄色ブドウ球菌死菌に対する免疫応答の場となる
  長澤 裕哉; 菊 佳男; 田邉 扶由子; 菅原 和恵; 石川 義春; 市居 修; 昆 泰寛; 門田 耕一; 林 智人
  第21回日本乳房炎研究会・学術集会, Oct. 2016, Japanese, Oral presentation
  [Domestic Conference]
• In vivo analysis of PCB metabolic capacities and effects on the thyroid hormone in cats
  Hazuki, Mizukawa; Misaki Maehara; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Kei Nomiyama; Hiroyuki Nishikawa; Kohki Takaguchi; Aksorn Saengtienchai; Tsend-ayush Sainnoxoi; Yoshinori Ikenaka; Shouta M.M. Nakayama; Shinsuke Tanabe; Mayumi Ishizuka
  The 9th International PCB Workshop, Oct. 2016, English, Oral presentation
  [International presentation]
• コットンラットの咽頭喉頭部,甲状腺及びその周囲組織にみられる形態学的特徴
  中村 鉄平; 入江 隆夫; 市居 修; 篠原 明男; 孝口 裕一; 越本 知大; 長崎 健一; 昆 泰寛
  日本解剖学会第62回東北・北海道連合支部学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• マウス十二指腸空腸曲の形態形成機序の解析
  尾之内佐和; 市居 修; 昆 泰寛
  日本解剖学会第62回東北・北海道連合支部学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• 自己免疫疾患が卵管の卵子捕捉機能に及ぼす影響について
  細谷 実里奈; 市居 修; 中村 鉄平; Yaser Hosny Ali Elewa; 昆 泰寛
  日本解剖学会第62回東北・北海道連合支部学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• 遠位尿細管上皮細胞の形態機能を制御するIL-1F6/IL-36α
  居 修; 中村鉄平; 岡村匡史; 木村純平; 堀野太郎; 昆 泰寛
  日本解剖学会第62回東北・北海道連合支部学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• Effect of H2S on 5-HT release from glomus cells in the chicken thoracic aorta
  Dugar DELGERMURUN; 山口 聡一郎; 市居 修; 昆 泰寛; 伊藤 茂男; 乙黒 兼一
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• 抗ワクモ（Dermanyssus gallinae）ワクチン開発に向けたワクモ由来フェリチン2の性状解析
  伊勢崎 政美; 村田 史郎; 酒井 英史; 矢吹 卓也; 種子野 章; 市居 修; 伊東 拓也; 今内 覚; 大橋 和彦
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• 芳香族炭化水素受容体シグナルの活性化は胃粘膜異常を増悪する
  團塚 愛; Annika Hanberg; 市居 修; 中村 鉄平; 昆 泰寛
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• マウス遠位尿細管の嚢胞形成に関与する新規原因遺伝子座の役割
  市居 修; 中村 鉄平; 矢吹 映; 堀野 太郎; 昆 泰寛
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• ウシ咽頭扁桃は鼻腔投与した黄色ブドウ球菌に対する免疫応答の場になる
  長澤 裕哉; 菊 佳男; 田邉 扶由子; 菅原 和恵; 石川 義春; 野地 智法; 市居 修; 昆 泰寛; 麻生 久; 門田 耕一; 林 智人
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• Histopathological correlations between the mediastinal fat associated lymphoid clusters and bleomycin-induced pneumonitis in mice
  Y. H. A. Elewa; Md. A. Masum; T. Nakamura; O. Ichii; Y. Kon
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• CD34-positive capillary decreased with the progression of kidney disease in mouse models
  Masum Md. Abdul; Elewa YH; 市居 修; 昆 泰寛
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• 自己免疫疾患モデルMRL/MpJ-Faslprマウスにみられる卵子のキャッチアップ障害
  細谷 実里奈; 市居 修; 中村 鉄平; Yaser Hosny Ali Elewa; 昆 泰寛
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• マウス胎 子腸管器官培養法により形成された十二指腸空腸曲の解析
  尾之内佐和; 市居 修; 昆 泰寛
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• コットンラットにおけるインスリン抵抗性と脂肪蓄積との関連
  中村 鉄平; 市居 修; 入江 隆夫; 寸田 祐嗣; 孝口 裕一; 長崎 健一; 吉安 友二; 昆 泰寛
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• 犬と猫の慢性腎臓病における尿細管周囲毛細血管の変化
  中村麗菜; 矢吹 映; 市居 修; 水川葉月; 横山 望; 大和 修
  第159回 日本獣医学会学術集会, Sep. 2016, Japanese, Oral presentation
  [Domestic Conference]
• Activation of Ahr signaling pathway aggravates the mucosal abnormality in stomach
  Ai Dantsuka; Annika Hanberg; Osamu Ichii; Yasuhiro Kon
  The 4th Sapporo Summer Seminar for One Health, Sep. 2016, English, Oral presentation
  [International presentation]
• CD34-positive capillary decreased with the progression of kidney disease in mice
  Masum Md. Abdul; Elewa YH; Ichii O; Kon Y
  The 4th Sapporo Summer Seminar for One Health, Sep. 2016, English, Oral presentation
  [International presentation]
• Analysis of the mouse duodenojejunal flexure using the fetal gut organ culture system
  Onouchi S; Ichii O; Kon Y
  The 4th Sapporo Summer Seminar for One Health, Sep. 2016, English, Oral presentation
  [International presentation]
• The effect of environmental pollutants to pet animals ~Level and effects of Persistent organic pollutants (POPs) to domestic ca
  Tsend-ayush Sainnokhoi; Hazuki Mizukawa; Misaki Maehara; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Kei Nomiyama; Aksorn Saengtienchai; Shinsuke Tanabe; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
  The 4th Sapporo Summer Seminar for One Health, Sep. 2016, English, Oral presentation
  [International presentation]
• イヌの尿中exosomeに含まれるマイクロRNA - 病態マーカーとしての可能性 ?
  市居修; 大田寛; 堀野太郎; 中村鉄平; 細谷実里奈; 溝口達也; 森下啓太郎; 中村健介; 星野有希; 高木哲; 佐々木東; 滝口満喜; 佐藤遼; 小山田和央; 昆泰寛
  第9回 日本獣医腎泌尿器学会学術集会, Aug. 2016, Japanese, Oral presentation
  [Domestic Conference]
• 雌性コットンラット(Sigmodon hispidus)にみられる腎性貧血
  中村 鉄平; 市居 修; 入江 隆夫; 孝口 裕一; 寸田 祐嗣; 堀野 太郎; 昆 泰寛
  第9回 日本獣医腎泌尿器学会学術集会, Aug. 2016, Japanese, Oral presentation
  [Domestic Conference]
• Metabolic capacities of polychlorinated biphenyls (PCBs) in cats and dog
  Hazuki Mizukawa; Kei Nomiyama; Shinsuke Tanabe; Misaki Maehara; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka, M
  The 8th international Toxicology symposium in Africa, Aug. 2016, English, Oral presentation
  [International presentation]
• ポリ塩化ビフェニル（PCBs）のin vivo暴露によるネコの異物代謝解明と甲状腺ホルモンへの影響評価
  水川 葉月; 前原 美咲; 横山 望; 市居 修; 滝口 満喜; 野見山 桂; 西川 博之; 池中 良徳; 中山 翔太; 高口 倖暉; 田辺 信介; 石塚 真由美
  第43回日本毒性学会学術年会, Jun. 2016, Japanese, Oral presentation
  [Domestic Conference]
• メタボローム解析を用いたPCBsによるイエネコの毒性影響評価
  西川博之; 野見山桂; 水川葉月; 横山望; 市居修; 滝口満喜; 石塚真由美; 池中良徳; 中山翔太; 江口哲史; 国末達也; 田辺信介
  第25回環境化学討論会, Jun. 2016, Japanese, Oral presentation
  [Domestic Conference]
• ネコにおけるポリ塩化ビフェニル（PCBs）の異物代謝機構解明と甲状腺ホルモンへの影響評価
  水川葉月; 前原美咲; 横山望; 市居修; 滝口満喜; 野見山桂; 西川博之; 池中良徳; 中山翔太; 高口倖暉; 田辺信介; 石塚真由美
  第25回環境化学討論会, Jun. 2016, Japanese, Oral presentation
  [Domestic Conference]
• Mouse duodenojejunal flexure formation with asymmetric morphologies between the inner and outer bending sides
  Onouchi S; Ichii O; Kon Y
  JSDB Special Symposium: Frontier of Developmental Biology Hosted by JSDB, Jun. 2016, English, Oral presentation
  [International presentation]
• SEM法を利用した腎生検の迅速電子顕微鏡診断への応用
  髙木 孝士; 城 謙輔; 大野 伸彦; 齊藤 成; Thai Quynh T; 昆 泰寛; 市居 修; Yeser Hosny Ali Elewa H; 笹野 公伸
  第105回日本病理学会総会, May 2016, Japanese, Oral presentation
  [Domestic Conference]
• Immune system via novel mediastinal fat associated lymphoid clusters in mice
  Y. H. A. Elewa; O. Ichii; Y. Kon
  The Scientific Symposium, Mar. 2016, English, Oral presentation
  [International presentation]
• Sex-related difference of the mediastinal fat associated lymphoid clusters in MRL/MpJ-Faslpr autoimmune disease model mice.
  Y. H. A. Elewa; O. Ichii; Y. Kon
  The 11th Scientific Vet. Med. (second international) conference, Mar. 2016, English, Oral presentation
  [International presentation]
• TO ACCELERATE A GLOBAL NETWORK OF ENVIRONMENTAL RESEARCHERS BIOTRANSFORMATION POTENCIES OF POLYCHLORINATED BIPHENYLS IN CATS
  Hazuki Mizukawa; Misaki Maehara; Nozomu Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Kei Nomiyama; Yoshinori Ikenaka; Shouta M.M.Nakayama; Shinsuke Tanabe; Mayumi Ishizuka
  INTERNATIONAL SYMPOSIUM ON ENVIRONMENTAL CHEMISTRY AND TOXICOLOGY, Mar. 2016, English, Oral presentation
  [International presentation]
• Metabolism and biotransformation of organohalogen compounds in the liver microsomes of cats and dogs
  Hazuki Mizukawa; Kei Nomiyama; Nozomu, Yokoyama; Osamu Ichii; Mitsuyoshi Takiguchi; Yoshinori Ikenaka; Shouta M.M; Nakayama; Misaki Maehara; Shinsuke Tanabe; Mayumi Ishizuka
  SETAC North America 36th Annual Meeting, Nov. 2015, English, Oral presentation
  [International presentation]
• ミニチュア・ダックスフンドの炎症性結直腸ポリープ症例の結直腸粘膜におけるToll-like receptor 2,4の局在解析
  横山 望; 大田 寛; 賀川 由美子; 山崎 淳平; 市居 修; Nisa Khoirun; 森田 智也; 大菅 辰幸; 佐々木 東; 森下 啓太郎; 中村 健介; 滝口 満喜
  第158回 日本獣医学会学術集会, Sep. 2015, Japanese, Oral presentation
  [Domestic Conference]
• マウス十二指腸空腸曲の細胞外基質は屈曲形成と共に変遷する
  尾之内佐和; 市居 修; 昆 泰寛
  第158回 日本獣医学会学術集会, Sep. 2015, Japanese, Oral presentation
  [Domestic Conference]
• 日本獣医学会学術集会 遠位尿細管傷害を知らせるマーカー分子“IL-1F6/IL-36a”の有用性
  市居 修; 中村 鉄平; 堀野 太郎; 木村 純平; 岡村 匡史; 昆 泰寛
  第158回 日本獣医学会学術集会, Sep. 2015, Japanese, Oral presentation
  [Domestic Conference]
• Gut morphogenesis in mice ?spatiotemporal distribution of the extracellular matrix changes during duodenojejunal flexure formation
  Onouchi S; Ichii O; Kon Y
  The 3rd Sapporo Summer Seminar for One Health, Sep. 2015, English, Oral presentation
  [International presentation]
• 尿中exosome由来microRNAの解析−動物種横断的腎障害マーカーになるか？−
  市居修; 大田寛; 堀野太郎; 中村鉄平; 細谷実里奈; 溝口達也; 森下啓太郎; 中村健介; 佐々木東; 滝口満喜; 佐藤遼; 小山田和央; 昆泰寛
  第8回 日本獣医腎泌尿器学会学術集会, Aug. 2015, Japanese, Oral presentation
  [Domestic Conference]
• Interleukin 1 family, member 6 is a useful histopathological diagnostic marker in acute kidney injury
  O. Ichii; D. Shiozuru; S. Otsuka-Kanazawa; Y. Kon
  The 5th Congress of the Asian Association of Veterinary Anatomists, Feb. 2015, English, Poster presentation
  [International presentation]
• Biased distribution of ovarian mast cells alter the early follicular development in postnatal MRL/MpJ mice
  T. Nakamura; O. Ichii; S. Otsuka-Kanazawa; K. Nagasaki; Y. Kon
  The 5th Congress of the Asian Association of Veterinary Anatomists, Feb. 2015, English, Poster presentation
  [International presentation]
• A novel mouse mediastinal fat-associated lymphoid tissue and its relationship with autoimmune disease
  Y. H.A. Elewa; S. Otsuka-Kanazawa; O. Ichii; Y. Kon
  The 5th Congress of the Asian Association of Veterinary Anatomists, Feb. 2015, English, Poster presentation
  [International presentation]
• Autosomal causative locus for production of testicular oocytes in MRL/MpJ mice
  Saori Otsuka-Kanazawa; Osamu Ichii; Yasuhiro Kon
  The 5th Congress of the Asian Association of Veterinary Anatomists, Feb. 2015, English, Poster presentation
  [International presentation]
• Validation of the function of MRL/MpJ mouse-derived loci in the spermatogenesis under testicular hyperthermia
  M. Chihara; S. Otsuka; O. Ichii; Y. Kon
  The 5th Congress of the Asian Association of Veterinary Anatomists, Feb. 2015, English, Poster presentation
  [International presentation]
• Spatiotemporal distribution of the extracellular matrix in the fetal mouse duodenojejunal flexure
  S. Onouchi; O. Ichii; S. Otsuka-Kanazawa; Y. Kon
  The 2nd Sapporo Summer Seminar for One Health, Feb. 2015, English, Poster presentation
  [International presentation]
• Greb1, a Gene Responsible for the Development of Renal Cysts Originating in the Distal Tubules in DBA/2 Mice
  Osamu Ichii; Saori Otsuka; Akira Yabuki; Taro Horino; Teppei Nakamura; Yasuhiro Kon
  American Society of Nephrology, Nov. 2014, English, Oral presentation
  [International presentation]
• Toll-Like Receptor 8 Contributes to Podocyte Injury in Murine Autoimmune Glomerulonephritis
  Junpei Kimura; Osamu Ichii; Teppei Nakamura; Taro Horino; Saori Otsuka; Yasuhiro Kon
  American Society of Nephrology, Nov. 2014, English, Oral presentation
  [International presentation]
• 足細胞の機能形態を制御するmiR-26a −糸球体傷害のバイオマーカー核酸になり得る−
  市居 修; 大塚沙織; 中村鉄平; 木村 純平; 堀野 太郎; 昆 泰寛
  第7回日本獣医腎泌尿器学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• miR-26a − 腎糸球体上皮細胞の機能形態を制御する短鎖RNA
  市居 修
  第157回 日本獣医学会学術集会 日本獣医解剖学会シンポジウム, Sep. 2014, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• Characterization of mouse mediastinal fat-associated lymphoid clusters (english)
  Yaser Hosny Ali Elewa; 市居 修; 大塚 沙織; 昆 泰寛
  第157回 日本獣医学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• Greb1 −DBA/2Crマウスに出現する遠位尿細管由来嚢胞の原因遺伝子候補−
  市居 修; 大塚 沙織; 矢吹 映; 堀野 太郎; 中村 鉄平; 昆 泰寛
  第157回 日本獣医学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• 自己免疫疾患モデルMRL/MpJ-lprマウスの卵巣は機能形態異常を示す
  大谷 祐紀; 市居 修; 大塚 沙織; 中村 鉄平; 千原 正尚; 昆 泰寛
  第157回 日本獣医学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• マウス十二指腸空腸曲における屈曲内−外側の形態学的差異
  尾之内 佐和; 市居 修; 大塚 沙織; 昆 泰寛
  第157回 日本獣医学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• 腎障害時にみられるMRL/MpJマウスの特別な修復過程について
  塩水流 大地; 市居 修; 中村 鉄平; 大塚 沙織; 昆 泰寛
  第157回 日本獣医学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• Toll-like receptor 8は自己免疫性糸球体腎炎の足細胞傷害に関与する
  木村 純平; 市居 修; 大田 寛; 中村 鉄平; 堀野 太郎; 大塚 沙織; 昆 泰寛
  第157回 日本獣医学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• 新生子MRL/MpJマウスにおける卵巣内肥満細胞の由来?卵巣-卵巣采結合部から出現する可能性?
  中村 鉄平; 千原 正尚; 市居 修; 大塚 沙織; 長崎 健一; 昆 泰寛
  第157回 日本獣医学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• MRL/MpJマウスに出現する精巣内卵細胞のエピゲノム解析
  大塚 沙織; 市居 修; 昆 泰寛
  第157回 日本獣医学会学術集会, Sep. 2014, Japanese, Oral presentation
  [Domestic Conference]
• Toll-like receptor 8 contributes to podocyte injury in murine autoimmune glomerulonephritis
  Junpei Kimura; Osamu Ichii; Saori Otsuka; Yasuhiro Kon
  The 2nd Sapporo Summer Seminar for One Health, Sep. 2014, English, Oral presentation
  [International presentation]
• 腎障害モデルにおける腎組織修復過程の観察 −MRL/MpJマウスが具備する創傷治癒形質との関連−
  塩水流大地; 市居 修; 中村鉄平; 大塚沙織; 昆 泰寛
  第7回日本獣医腎泌尿器学会学術集会, Aug. 2014, Japanese, Oral presentation
  [Domestic Conference]
• 伴侶動物の腎臓におけるToll-like receptor8の発現解析 -糸球体足細胞傷害に関与するバイオセンサー-
  木村 純平; 市居 修; 大田 寛; 横山 望; 森下 啓太郎; 星野 有希; 高木 哲; 大塚 沙織; 昆 泰寛
  第7回日本獣医腎泌尿器学会学術集会, Aug. 2014, Japanese, Oral presentation
  [Domestic Conference]
• The candidate factors for the development of chronic kidney disease.
  Osamu Ichii; Saori Otsuka; Yasuhiro Kon
  The 16th SNU-HU Symposium Current Advances in Veterinary Medicine., Dec. 2013, English, Oral presentation
  [International presentation]
• MRL/MpJマウスに出現する肥満細胞
  中村鉄平; 市居 修; 大塚沙織; 昆 泰寛
  日本解剖学会第59回東北・北海道連合支部学術集会, Sep. 2013, Japanese, Invited oral presentation
  [Domestic Conference]
• マウスにおける十二指腸空腸曲形成機序の解析
  尾之内佐和; 市居 修; 大塚沙織; 昆 泰寛
  日本解剖学会第59回東北・北海道連合支部学術集会, Sep. 2013, Japanese, Oral presentation
  [Domestic Conference]
• 伴侶動物の腎臓に発現するmiRNAのデータベース構築−動物種共通のバイオマーカー開発を目指して
  市居 修; 大塚沙織; 矢吹 映; 大田 寛; 堀野太郎; 昆 泰寛
  第156回 日本獣医学会学術集会, Sep. 2013, Japanese, Oral presentation
  [Domestic Conference]
• 慢性膵炎の系統差に関する研究-MRLマウス1番染色体テロメア領域の影響
  岡田祐樹; 市居 修; 大塚 沙織; 昆 泰寛
  第156回 日本獣医学会学術集会, Sep. 2013, Japanese, Oral presentation
  [Domestic Conference]
• MRL/MpJマウスの卵細胞発育を制御する遺伝学的因子
  山下由真; 市居 修; 大塚沙織; 昆 泰寛
  第156回 日本獣医学会学術集会, Sep. 2013, Japanese, Oral presentation
  [Domestic Conference]
• ビタミンA欠乏食給餌マウスからみる精細胞−血液精巣関門の相互作用
  千原正尚; 市居 修; 大塚沙織; 昆 泰寛
  第156回 日本獣医学会学術集会, Sep. 2013, Japanese, Oral presentation
  [Domestic Conference]
• マウス消化管の発生に関する研究 〜どうして腸管は曲がるのか?〜
  尾之内 佐和; 市居 修; 大塚 沙織; 橋本 善春; 昆 泰寛
  第154回 日本獣医学会学術集会, Sep. 2013, Japanese, Oral presentation
  [Domestic Conference]
• The uremic toxin indoxyl sulfate causes podocyte injury in vivo and in vitro
  Osamu Ichii; Huiyan Lu; Hideko Takahashi; Patricia Zerfas; Jeffrey Kopp
  Kidney week 2013, American Society of Nephrology, Sep. 2013, English
  [International presentation]
• 腎臓病のmicroRNA解析―動物種共通のバイオマーカー開発を目指して―
  市居 修; 大塚沙織; 中村鉄平; 堀野太郎; 昆 泰寛
  第6回日本獣医腎泌尿器学会学術集会, Aug. 2013, Japanese, Oral presentation
  [Domestic Conference]
• 糸球体腎炎モデルマウスの足細胞傷害 - Toll-like receptor ファミリーの関与
  木村純平; 市居 修; 大塚沙織; 昆 泰寛
  第6回日本獣医腎泌尿器学会学術集会, Aug. 2013, Japanese, Oral presentation
  [Domestic Conference]
• 糸球体病変を制御するmiRNAの探索−動物種共通のバイオマーカー開発を目指して−
  市居 修; 大塚沙織; 中村鉄平; 野元由佳; 橋本善春; 堀野太郎; 昆 泰寛
  第155回 日本獣医学会学術集会, Mar. 2013, Japanese, Poster presentation
  [Domestic Conference]
• 糸球体腎炎モデルマウスにおける足細胞傷害の解析
  木村純平; 市居 修; 大塚沙織; 佐々木隼人; 橋本善春; 昆 泰寛
  第155回 日本獣医学会学術集会, Mar. 2013, Japanese, Poster presentation
  [Domestic Conference]
• 熱ショック誘導性の精細管腔内石灰沈着の発生機序と抑制因子の解析
  千原正尚; 市居 修; 大塚沙織; 橋本善春; 昆 泰寛
  第155回 日本獣医学会学術集会, Mar. 2013, Japanese, Poster presentation
  [Domestic Conference]
• MRLマウス肥満細胞は新生子期の卵形成に関与する
  中村鉄平; 市居 修; 大塚沙織; 坂田侑子; 長崎健一; 橋本善春; 昆 泰寛
  第155回 日本獣医学会学術集会, Mar. 2013, Japanese, Oral presentation
  [Domestic Conference]
• Initiation of meiosis in fetal testis of MRL mouse to produce testicular oocyte
  Saori Otsuka; Osamu Ichii; Nobuya Sasaki; Yoshiharu Hashimoto; Yasuhiro Kon
  第4回アジア獣医解剖学会, Oct. 2012, English, Oral presentation
  [International presentation]
• Mast cells in neonatal ovary -a new unique phenotype of MRL/MpJ mice-
  Teppei NAKAMURA; Yuko SAKATA; S. Saori OTSUKA; Osamu ICHII; Kenichi NAGASAKI; Yoshiharu HASHIMOTO; Yasuhiro KON
  第4回アジア獣医解剖学会, Sep. 2012, English, Oral presentation
  [Domestic Conference]
• Analysis of claudin-3 expression and function during the murine spermatogenesis.
  Masataka CHIHARA; Saori OTSUKA; Osamu ICHII; Yoshiharu HASHIMOTO; Yasuhiro KON
  第4回アジア獣医解剖学会, Sep. 2012, English, Oral presentation
  [International presentation]
• 尿毒症物質インドキシル硫酸は腎糸球体上皮細胞傷害に関与する―芳香族炭化水素受容体のリガンド作用―
  市居 修; 若新 英史; 村上 太一; 堀野 太郎; Huiyan Lu; Hideko Takahashi; Zerfas Patricia; 大塚 沙織; 橋本 善春; 昆 泰寛; Jeffrey Kopp
  第154回 日本獣医学会学術集会, Sep. 2012, Japanese, Oral presentation
  [Domestic Conference]
• 高温曝露後の精巣における減数分裂の進行と精細管腔内に出現する石灰沈着
  千原 正尚; 市居 修; 大塚 沙織; 橋本 善春; 昆 泰寛
  第154回 日本獣医学会学術集会, Sep. 2012, Japanese, Oral presentation
  [Domestic Conference]
• マレック病感染鶏由腫瘍病変部における免疫抑制因子PD-1/PD-L1発現の形態学的解析
  村田史郎; 松山あゆみ; 伊勢崎 政美; 高崎紗蘭; 市居 修; 今内 覚; 大橋和彦
  第154回 日本獣医学会学術集会, Sep. 2012, Japanese, Oral presentation
  [Domestic Conference]
• MRLマウス胎子精巣における卵細胞形成過程の解析
  大塚沙織; 市居 修; 佐々木宣哉; 橋本善春; 遠藤大二; 昆 泰寛
  第58回東北・北海道連合支部学術集会, Aug. 2012, Japanese, Oral presentation
  [Domestic Conference]
• Indoxyl sulfate: a uremic toxin and aryl-hydrocarbon receptor ligand that may cause podocyte injury
  Osamu Ichii; Jeffrey B Kopp
  National Kidney Foundation, 21st Annual Fellows Research Forum, 13 Jun. 2012, English
  [International presentation]
• Indoxyl sulfate: a uremic toxin and aryl-hydrocarbon receptor ligand that may cause podocyte injury
  Osamu Ichii; Jeffrey Kopp
  The 7th annual NIDDK Fellows Retreat, NIH, Apr. 2012, English, Poster presentation
  [International presentation]
• MRL/MpJマウスの卵巣内肥満細胞出現に関与する遺伝的因子の解析
  中村鉄平; 坂田侑子; 大塚沙織; 市居 修; 並木由佳; 長崎健一; 橋本善春; 昆 泰寛
  第153回 日本獣医学会学術集会, Mar. 2012, Japanese, Poster presentation
  [Domestic Conference]
• マウス精巣における密着結合蛋白claudin-3の局在および機能解析
  千原正尚; 市居 修; 大塚沙織; 並木由佳; 池渕良洋; 橋本善春; 昆 泰寛
  第153回 日本獣医学会学術集, Mar. 2012, Japanese, Oral presentation
  [Domestic Conference]
• High-Resolution Linkage Mapping of the Rat Hooded Locus
  Torigoe Daisuke; Ichii Osamu; Ruihua Dang; Ohnaka Takayuki; Okano Shinya; Kon Yasuhiro; Sasaki Nobuya; Agui Takashi
  61st The American Association for Laboratory Animal Science, Oct. 2011, English, Oral presentation
  [International presentation]
• 節足動物DNAのリボゾームRNA遺伝子ITS領域のサイズ分析による生物種同定の試み
  佐藤福太郎; 遠藤大二; 吉村貴行; 伊東拓也; 長 雄一; 浅川満彦; 昆 泰寛; 市居 修; 大塚沙織; 林 正信
  第152回 日本獣医学会学術集会, Sep. 2011, Japanese, Oral presentation
  [Domestic Conference]
• マウス涙腺炎における涙液を使った病態解析
  小千田圭吾; 市居 修; 大塚沙織; 並木由佳; 橋本善春; 昆 泰寛
  第152回 日本獣医学会学術集会, Sep. 2011, Japanese, Oral presentation
  [Domestic Conference]
• マウス心筋発生過程に出現するTUNEL陽性細胞の解析
  長沼佑季; 市居 修; 大塚沙織; 並木由佳; 橋本善春; 昆 泰寛
  第152回 日本獣医学会学術集会, Sep. 2011, Japanese, Oral presentation
  [Domestic Conference]
• MRLマウス新生子卵巣に出現する肥満細胞の系統差解
  中村鉄平; 市居 修; 大塚沙織; 並木由佳; 坂田侑子; 長崎健一; 服部秀樹; 橋本善春; 昆 泰寛
  第152回 日本獣医学会学術集会, Sep. 2011, Japanese, Oral presentation
  [Domestic Conference]
• 糸球体腎炎モデルマウスにおける尿中脱落細胞の動態解析
  木村純平; 市居 修; 大塚沙織; 金澤智則; 並木由佳; 橋本善春; 昆 泰寛
  第57回 日本解剖学会 東北・北海道連合支部学術集会, Aug. 2011, Japanese, Oral presentation
  [Domestic Conference]
• ラット頭巾斑責任遺伝子の同定
  鳥越 大輔; 市居 修; 党 瑞華; 佐々木 宣哉; 昆 泰寛; 安居院 高志
  北海道実験動物研究会・日本実験動物技術者協会北海道支部 合同学術集会 2011, Jul. 2011, Japanese, Oral presentation
  [Domestic Conference]
• 節足動物DNAのITS領域サイズ分析による生物種同定の試み
  遠藤大二; 吉村貴行; 佐藤福太郎; 伊東拓也; 長 雄一; 浅川満彦; 昆 泰寛; 市居 修; 大塚沙織; 林 正信
  第151回 日本獣医学会学術集会, Mar. 2011, Japanese, Poster presentation
  [Domestic Conference]
• 腎症候性出血熱の致死的感染モデルの開発とその病態解析
  瀬戸隆弘; 吉川佳佑; 真田崇弘; Ngonda Saasa; 尾崎由佳; 市居 修; 好井健太朗; 昆 泰寛; 苅和宏明
  第151回 日本獣医学会学術集会, Mar. 2011, Japanese
  [Domestic Conference]
• MRL/MpJマウスにおける卵巣網由来卵巣?腫の経時的観察
  李 愼曉; 市居 修; 大塚沙織; 橋本善春; 昆 泰寛
  第151回 日本獣医学会学術集会, Mar. 2011, Japanese
  [Domestic Conference]
• MRLマウス新生子卵巣における肥満細胞の出現
  中村鉄平; 市居 修; 大塚沙織; 並木由佳; 長崎健一; 服部秀樹; 橋本善春; 昆 泰寛
  第151回 日本獣医学会学術集会, Mar. 2011, Japanese
  [Domestic Conference]
• 129+Ter/Svマウスの遺伝的背景は腎糸球体硬化症に抵抗性である
  西野智博; 佐々木宣哉; 長崎健一; 市居 修; 昆 泰寛; 安居院高志
  第151回 日本獣医学会学術集, Mar. 2011, Japanese, Poster presentation
  [Domestic Conference]
• 慢性腎臓病におけるmicroRNAの発現解析−腎間質病変に関与するmiR-146a−
  市居 修; 大塚沙織; 並木由佳; 佐々木宣哉; 橋本善春; 遠藤大二; 昆 泰寛
  第151回 日本獣医学会学術集会, 2011, Japanese, Oral presentation
  [Domestic Conference]
• マウス血液精巣関門関連分子の局在と動態 ―claudin-3, -11, occludin, ZO-1の発現解析
  千原正尚; 市居修; 大塚沙織; 橋本善春; 昆泰寛
  第56回日本解剖学会 東北・北海道連合支部学術集会, Sep. 2010, Japanese, Oral presentation
  [Domestic Conference]
• MRLマウスの精巣内卵細胞出現に関与する遺伝的因子の解析
  大塚沙織; 市居 修; 佐々木宣哉; 並木 由佳; 橋本善春; 遠藤大二; 昆 泰寛
  第150回 日本獣医学会学術集会, Sep. 2010, Japanese, Oral presentation
  [Domestic Conference]
• 尿中脱落細胞の動態は腎疾患の増悪と相関する‐糸球体腎炎モデルの解析
  木村純平; 市居 修; 大塚沙織; 金澤智則; 並木由佳; 橋本善春; 昆 泰寛
  第150回 日本獣医学会学術集会, Sep. 2010, Japanese, Oral presentation
  [Domestic Conference]
• Comparative analysis of the expression of aquaporin family between pre- and post-weaning periods in goat major salivary glands
  Elewa Yaser; 市居 修; 大塚沙織; 金澤智則; 李 愼曉; 並木由佳; 昆 泰寛; 橋本善春
  第150回 日本獣医学会学術集会, Sep. 2010, Japanese, Oral presentation
  [Domestic Conference]
• 慢性腎臓病における糸球体上皮細胞傷害の解析―腎機能とスリット膜関連分子の動態はイヌとネコで異なる―
  市居 修; 矢吹 映; 佐々木宣哉; 大塚沙織; 大田 寛; 山崎真大; 滝口満喜; 並木由佳; 橋本善春; 遠藤大二; 昆 泰寛
  第3回 日本獣医泌尿器学会学術集会, Jul. 2010, Japanese, Oral presentation
  [Domestic Conference]
• MRLマウス胎子精巣内における減数分裂開始と精巣内卵細胞の出現
  大塚沙織; 市居 修; 佐々木宣哉; 橋本善春; 遠藤大二; 昆 泰寛
  第149回 日本獣医学会学術集会, Mar. 2010, Japanese, Poster presentation
  [Domestic Conference]
• Age related structural changes of parotid salivaly glands in goat
  Yaser Elewa; 橋本善春; 市居 修; 大塚沙織; 金澤智則; 昆 泰寛
  第149回 日本獣医学会学術集会, Mar. 2010, English, Poster presentation
  [Domestic Conference]
• マウスのネフロン形成過程における間葉‐上皮転換（MET）の解
  金澤智則; 大塚沙織; 市居 修; 橋本善春; 昆 泰寛
  第149回 日本獣医学会学術集, Mar. 2010, Japanese, Poster presentation
  [Domestic Conference]
• Pathological differences between dog and cat in renal disease -slit membrane molecules and renal dysfucntion-
  149th Meeting of the Japanease Society of Veterinary Science, 2010, Poster presentation
• DBA/2マウスの腎臓における遠位尿細管上皮傷害 -多発性嚢胞腎の早期病変としての可能性-
  市居 修; 大塚沙織; 矢吹 映; 佐々木宣哉; 並木由佳; 橋本善春; 遠藤大二; 昆 泰寛
  第150回 日本獣医学会学術集会, 2010, Japanese, Oral presentation
  [Domestic Conference]
• イヌおよびネコの腎疾患における病態差 −腎機能とスリット膜関連分子の動態−
  市居 修; 佐々木宣哉; 矢吹 映; 大田 寛; 滝口満喜; 大塚沙織; 橋本善春; 遠藤大二; 昆 泰寛
  第149回日本獣医学会学術集会, 2010, Japanese, Poster presentation
  [Domestic Conference]
• マウス精子形成過程における血液精巣関門構成分子の動態
  千原正尚; 市居修; 大塚沙織; 橋本善春; 昆 泰寛
  平成21年度 日本顕微鏡学会 北海道支部学術講演会, Dec. 2009, Japanese, Oral presentation
  [Domestic Conference]
• MRL/MpJマウスに出現する精巣内卵細胞の解析
  大塚沙織; 市居 修; 佐々木宣哉; 橋本善春; 遠藤大二; 昆 泰寛
  平成21年度 日本顕微鏡学会, Dec. 2009, Japanese, Oral presentation
  [Domestic Conference]
• Age-related structural changes of parotid salivary glands in goat.
  Elewa Y; Ichii O; Otsuka S; Kanazawa T; Kon Y; Hashimoto Y
  平成21年度 日本顕微鏡学会, Dec. 2009, English, Oral presentation
  [Domestic Conference]
• Quantitative trait loci analysis of development of ovarian cyst originated from rete ovarii
  李 愼曉; 市居 修; 大塚沙織; 橋本善春; 昆 泰寛
  第3回 アジア獣医解剖学会, Nov. 2009, English, Poster presentation
  [International presentation]
• マウス精子形成過程における血液精巣関門構成分子の動態
  千原正尚; 大塚沙織; 市居 修; 橋本善春; 昆 泰寛
  第148回 日本獣医学会学術集会, Nov. 2009, Japanese, Oral presentation
  [Domestic Conference]
• Development of innovative method for diagnosis renal disease - Analysis of urinary cells in model mice -
  木村純平; 市居 修; 大塚沙織; 橋本善春; 昆 泰寛
  Hokkaido University International Symposium on Sustainability Weeks 2009, Nov. 2009, Japanese, Poster presentation
  [Domestic Conference]
• MRL/MpJマウスに見られる卵巣網由来卵巣嚢腫の量的形質遺伝子座解析
  李 愼曉; 市居 修; 大塚沙織; 橋本善春; 昆 泰寛
  第147回 日本獣医学会学術集会, Apr. 2009, Japanese, Poster presentation
  [Domestic Conference]
• Hydronephrosis in (C57BL/6 x DBA/2) mice
  148th Meeting of the Japanease Society of Veterinary Science, 2009
• New diagnosis method for renal disease -Interleukin 1 family member 6 is a biomarker for tubular injury-
  147th Meeting of the Japanease Society of Veterinary Science, 2009
• (DBA/2 x C57BL/6) F2交雑マウスに出現する水腎症
  市居 修; 昆 泰寛; 佐々木宣哉; 矢吹 映; 大塚沙織; 橋本善春; 遠藤大二
  第148回日本獣医学会学術集会, 2009, Japanese, Oral presentation
  [Domestic Conference]
• 腎疾患の新しい診断指標 −Interleukin 1 family, member 6は尿細管傷害マーカーになり得る
  市居 修; 大塚沙織; 佐々木宣哉; 矢吹 映; 大田 寛; 滝口満喜; 橋本善春; 遠藤大二; 昆 泰寛
  第147回日本獣医学会学術集会, 2009, Japanese, Oral presentation
  [Domestic Conference]
• テンシン2遺伝子の糸球体上皮細胞における機能解析
  佐々木宣哉; 市居 修; 昆 泰寛; 安居院高志
  第146回 日本獣医学術集会, Sep. 2008, Japanese, Oral presentation
  [Domestic Conference]
• The relations between autoimmune glomerulonephritis and Interferon activated gene 200 (Ifi200).
  146th Meeting of the Japanease Society of Veterinary Science, 2008
• The effects of gonadectomy on the pathogenesis of glomerulonephritis.
  145th Meeting of the Japanease Society of Veterinary Science, 2008, Poster presentation
• Interferon activated gene 200 (Ifi200)遺伝子群と自己免疫性糸球体腎炎の関連性
  市居修; 上川昭博; 佐々木宣哉; 今野明弘; 遠藤大二; 橋本善春; 昆 泰寛
  第146回日本獣医学会学術集会, 2008, Japanese, Oral presentation
  [Domestic Conference]
• 性腺摘出が自己免疫性糸球体腎炎の病態に与える影響について
  市居 修; 今野明弘; 佐々木宣哉; 遠藤大二; 橋本善春; 昆 泰寛
  第145回日本獣医学会学術集会, 2008, Japanese, Oral presentation
  [Domestic Conference]
• The relations between autoimmune glomerulonephritis and Fc gamma receptor III（FcγRIII）.
  144th Meeting of the Japanease Society of Veterinary Science, 2007
• 自己免疫性糸球体腎炎と Fc gamma receptor III（FcγRIII）の関連性について
  市居 修; 今野明弘; 佐々木宣哉; 遠藤大二; 橋本善春; 昆 泰寛
  第144回日本獣医学会学術集会, 2007, Japanese, Oral presentation
  [Domestic Conference]
• Autoimmune glomerulonephritis in MRL-derived congenic mice.
  142th Meeting of the Japanease Society of Veterinary Science, 2006
• MRL/MpJマウス由来コンジェニックマウスに出現する自己免疫性糸球体腎炎
  市居 修; 今野明弘; 橋本善春; 昆 泰寛
  第142回日本獣医学会学術集会, 2006, Japanese, Oral presentation
  [Domestic Conference]
• Speciese-specific differences of laboratory redent kidenys- lenghts of nephrons, GFR associated factors, water channels and sodium pumps.
  140th Meeting of the Japanease Society of Veterinary Science, 2005
• 齧歯目の腎臓における種差―ネフロン長,GFR調節因子,水チャネルおよびナトリウムポンプについて
  市居 修; 矢吹 映; 小嶋敏慶; 松元光春; 鈴木秀作
  第140回日本獣医学会学術集会, 2005, Japanese, Oral presentation
  [Domestic Conference]
• Speciese-specific differences of renal structures in laboratory animals.
  22th Meeting of the Kyusyu Experimental Animal Research Association, 2004
• 実験動物の腎臓の種差に関する形態計測学的研究
  市居 修; 矢吹 映; 小嶋敏慶; 松元光春; 鈴木秀作
  第22回九州実験動物研究会総会, 2004, Japanese, Oral presentation
  [Domestic Conference]
• ウシ結膜におけるIgA陽性細胞の局在
  小千田圭吾; 市居修; 山下祐輔; 昆泰寛
  第2回獣医解剖アカデミア
• 雌性コットンラットの胸腺にみられる加齢性変化
  中村鉄平; 市居修; 入江隆夫; 篠原明男; 孝口裕一; 越本知大; 長崎健一; 寸田祐嗣; Yaser Hosny Ali Elewa; 辰巳治; 昆泰寛
  第160回日本獣医学会