Researcher Profile and Settings

Affiliation

• Research Institute for Electronic Science　Biology and Life Sciences

Job Title

• Assistant Professor

URL

http://chem.es.hokudai.ac.jp

Research funding number

• 50722716

J-Global ID

• 201801018621122945

Research Areas

• Nanotechnology/Materials / Nanomaterials

Educational Organization

•  Master's degree program　Graduate School of Life Science
•  Doctoral (PhD) degree program　Graduate School of Life Science

Academic & Professional Experience

• 2018/04 - Today Hokkaido University Research Institute for Electronic Science
• 2016/01 - 2018/03 九州大学大学院 化学工学部門 特任助教
• 2013/07 - 2015/12 物質・材料研究機構 超分子ユニット 博士研究員
• 2012/07 - 2013/06 ミシガン大学 歯学部 博士研究員
• 2010/04 - 2012/06 カリフォルニア大学アーバイン校 化学科 博士研究員

Education

• 2007/04 - 2010/03  東京工業大学大学院
• 2005/04 - 2007/03  東京工業大学大学院
• 2001/04 - 2005/03  Tokyo Institute of Technology  School of Bioscience and Biotechnology

Research Activities

Published Papers

• Dynamic Orientation Control of Gold Nanorods in Polymer Brushes by Their Thickness Changes for Plasmon Switching

  Yu Sekizawa, Yuka Hasegawa, Hideyuki Mitomo, Chisato Toyokawa, Yusuke Yonamine, Kuniharu Ijiro

  Advanced Materials Interfaces 2196-7350 2024/01/10 

  Abstract Gold nanorods (AuNRs) have unique optical properties such as transverse and longitudinal localized surface plasmon resonance (T‐ and L‐LSPR). As the L‐LSPR absorption depends on the angle of the AuNRs to incident light and polarization, orientational control of AuNRs is a crucial issue. In spite of various techniques to control AuNR orientation, dynamic orientation tuning on a solid substrate remains challenging. Herein, dynamic changes are demonstrated in AuNR orientation in the anionic polymer (DNA) brushes via control of their thickness by salt concentration. AuNRs vertically align toward the substrates when their thickness exceeds the AuNR length. Once their thickness becomes shorter than the AuNR length, the attached AuNRs begin to tilt. The tilt reaches a maximum level close to horizontal when the thickness decreases to half that of the AuNR length. The dynamic control between the vertical (uniform) and tilted (random) orientation of the AuNRs showed not only absorption intensity changes in L‐LSPR but also the switching of side‐by‐side plasmon coupling. The polymer brush‐based system affords a novel platform for the stimuli‐responsive control of AuNR orientation on the substrates via changes in the thickness of polymer brushes for actively tunable plasmonic substrates.
• Hysteresis in the Thermo-Responsive Assembly of Hexa(ethylene glycol) Derivative-Modified Gold Nanodiscs as an Effect of Shape

  Joshua Chidiebere Mba, Hideyuki Mitomo, Yusuke Yonamine, Guoqing Wang, Yasutaka Matsuo, Kuniharu Ijiro

  NANOMATERIALS 12 (9) 2022/05 [Refereed]
   

  Anisotropic gold nanodiscs (AuNDs) possess unique properties, such as large flat surfaces and dipolar plasmon modes, which are ideal constituents for the fabrication of plasmonic assemblies for novel and emergent functions. In this report, we present the thermo-responsive assembly and thermo-dynamic behavior of AuNDs functionalized with methyl-hexa(ethylene glycol) undecane-thiol as a thermo-responsive ligand. Upon heating, the temperature stimulus caused a blue shift of the plasmon peak to form a face-to-face assembly of AuNDs due to the strong hydrophobic and van der Waals interactions between their large flat surfaces. Importantly, AuNDs allowed for the incorporation of the carboxylic acid-terminated ligand while maintaining their thermo-responsive assembly ability. With regard to their reversible assembly/disassembly behavior in the thermal cycling process, significant rate-independent hysteresis, which is related to their thermo-dynamics, was observed and was shown to be dependent on the carboxylic acid content of the surface ligands. As AuNDs have not only unique plasmonic properties but also high potential for attachment due to the fact of their flat surfaces, this study paves the way for the exploitation of AuNDs in the development of novel functional materials with a wide range of applications.
• Core-Gap-Shell Nanoparticles@Polyaniline with TunablePlasmonic Chiroptical Activities by pH and Electric Potential Dual Modulation

  Han Lin, Hideyuki Mitomo, Yusuke Yonamine, Zhiyong Guo, Kuniharu Ijiro

  CHEMISTRY OF MATERIALS 34 (9) 4062 - 4072 0897-4756 2022/05 [Refereed]
   

  The emerging concept of tunable plasmonicchirality is mostly observed as a reconfigurable behavior or afeature of complex chiral plasmonic assemblies. For discretecolloidal particles, it is challenging to achieve reversible tunabilityor a transient response with regard to chiroptical activities,particularly in the visible or near-infrared region. Herein, wedemonstrate a stimulus-responsive system based on chiralmolecule-achiral plasmonic nanoparticles coated with polyaniline(PANI) as a variable dielectric layer, in whichL-/D-cysteinemolecules are introduced between the gold core and the shell as astatic chiral source, allowing the chiral transfer effect to be greatlyamplified by the hotspot gap of sub-monomolecular thickness. By taking advantage of the responsive properties to either pH or theelectric potential dual stimuli of PANI, which also provides a stable and real-time switchable dielectric environment for the wholesystem, dynamic tuning of the plasmon and its induced chiroptical activities of core-gap-shell nanoparticles@PANI were preciselyobtained. This well-defined design provides an open platform forflexible and rational tailoring of plasmonic cores, chiral molecules,and variable dielectrics to chiroptical needs, which is important for realizing applications in chemical sensing, chiral nanocatalysis,enantioselective separations, and novel optical devices
• Self-assembly of Gold Nanorods into a Highly Ordered Sheet via Electrostatic Interactions with Double-stranded DNA

  Satoshi Nakamura, Hideyuki Mitomo, Shigeaki Suzuki, Yu Torii, Yu Sekizawa, Yusuke Yonamine, Kuniharu Ijiro

  CHEMISTRY LETTERS 51 (5) 529 - 532 0366-7022 2022/05 [Refereed]
   

  Assemblies of cationic gold nanorods (AuNRs) via electrostatic interactions with double-stranded (ds) DNA were investigated in solution and after evaporation as a cast-film. Interestingly, moderately positively charged AuNRs assembled with dsDNA provided a monolayer sheet with an ordered alignment resembling a two-dimensional (2D) smectic structure during solvent evaporation.
• Probing the Biogenesis of Polysaccharide Granules in Algal Cells at Sub-Organellar Resolution via Raman Microscopy with Stable Isotope Labeling.

  Yusuke Yonamine, Takuya Asai, Yuta Suzuki, Takuro Ito, Yasuyuki Ozeki, Yu Hoshino

  Analytical chemistry 93 (50) 16796 - 16803 2021/12/21 [Refereed]
   

  Phototrophs assimilate CO2 into organic compounds that accumulate in storage organelles. Elucidation of the carbon dynamics of storage organelles could enhance the production efficiency of valuable compounds and facilitate the screening of strains with high photosynthetic activity. To comprehensively elucidate the carbon dynamics of these organelles, the intraorganellar distribution of the carbon atoms that accumulate at specific time periods should be probed. In this study, the biosynthesis of polysaccharides in storage organelles was spatiotemporally probed via stimulated Raman scattering (SRS) microscopy using a stable isotope (13C) as the tracking probe. Paramylon granules (a storage organelle of β-1,3-glucan) accumulated in a unicellular photosynthetic alga, Euglena gracilis, were investigated as a model organelle. The carbon source of the culture medium was switched from NaH12CO3 to NaH13CO3 during the production of the paramylon granules; this resulted in the distribution of the 12C and 13C constituents in the granules, so that the biosynthetic process could be tracked. Taking advantage of high-resolution SRS imaging and label switching, the localization of the 12C and 13C constituents inside a single paramylon granule could be visualized in three dimensions, thus revealing the growth process of paramylon granules. We propose that this method can be used for comprehensive elucidation of the dynamic activities of storage organelles.
• Molecular configuration-mediated thermo-responsiveness in oligo(ethylene glycol) derivatives attached on gold nanoparticles dagger

  Kun Xiong, Hideyuki Mitomo, Xueming Su, Yier Shi, Yusuke Yonamine, Shin-ichiro Sato, Kuniharu Ijiro

  NANOSCALE ADVANCES 3 (13) 3762 - 3769 2516-0230 2021/07 [Refereed]
   

  Biomolecular systems actively control their local environment on a sub-nm scale via changes in molecular configuration from their flexible structures and derive emergent functions. Although this functional emergence based on local environmental control is attracting a great deal of attention in chemistry, it remains challenging to realize this artificially. Herein, we report the tuning of the thermo-responsive properties of oligo(ethylene glycol) (OEG) derivatives attached on gold nanoparticles via local environmental control not only by the hydrophobic moiety at their terminus but also by their molecular configuration. OEG-attached alkane thiol-modified AuNPs showed thermo-responsive assembly/disassembly in water through the hydration/dehydration of the OEG portions in a manner dependent both on the hydrophobicity at their terminus and the surface curvature of the core nanoparticles. Further, the assembly temperature (T-A) was also tuned by ligand mixing with a non-thermo-responsive ligand with a shorter OEG length. Molecular dynamics simulations show that the distribution of the hydrophobic terminus in the normal direction along the gold surface varied in accordance with the surface curvature, indicating variations in molecular configuration. It is expected that a bent configuration could accelerate the thermo-responsiveness of OEG by allowing them greater accessibility to the hydrophobic terminus. Experimental and simulation results support the notion that local OEG density tuning by surface curvature or ligand mixing with a different OEG length leads to different degrees of accessibility to the hydrophobic terminus via changes in molecular configuration, promoting local environmental control-directed assembly temperature tuning.
• Reversible changes in the orientation of gold nanorod arrays on polymer brushes

  Yu Sekizawa, Hideyuki Mitomo, Mizuki Nihei, Satoshi Nakamura, Yusuke Yonamine, Akinori Kuzuya, Takehiko Wada, Kuniharu Ijiro

  NANOSCALE ADVANCES 2 (9) 3798 - 3803 2516-0230 2020/09 [Refereed][Not invited]
   

  Nanoparticles exhibit a number of unique properties such as localized surface plasmon resonance (LSPR). As this LSPR is sensitive to geometrical or spatial conditions, the arrangement of nanoparticles, in particular the active arrangement of plasmonic structures, is an important issue. In this study, gold nanorod (GNR) arrays were prepared by GNR attachment on anionic polymer (DNA) brushes via electrostatic interactions and their stimuli-responsive changes in orientationwere investigated. As a result, the orientation of GNR arrays on DNA brushes reversibly changed by the modulation of electrostatic interactions between GNRs and polymers via changes in the solution pH. As these extensive GNR arrays are prepared via easy bottom-up processes, GNR surface properties are easily tuned by simple modification, and DNAs could be replaced with various synthetic polymers, we believe that this study will lead to the development of nextgeneration materials and devices with actively tunable structures.
• Raman image-activated cell sorting.

  Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda

  Nature communications 11 (1) 3452 - 3452 2020/07/10 [Refereed][Not invited]
   

  The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.
• Salt-triggered Active Plasmonic Systems Based on the Assembly/Disassembly of Gold Nanorods in a DNA Brush Layer on a Solid Substrate

  Satoshi Nakamura, Hideyuki Mitomo, Yusuke Yonamine, Kuniharu Ijiro

  CHEMISTRY LETTERS 49 (7) 749 - 752 0366-7022 2020/07 [Refereed][Not invited]
   

  In this study, we demonstrate that the plasmonic properties of gold nanorods (GNRs) electrostatically adsorbed on a DNA brush substrate are reversibly controlled by changes in NaCl concentration. This plasmonic change results from GNR assembly/disassembly in a DNA brush layer. In addition, we show that this active plasmonic system exhibits intense and switchable chiroptical properties.
• Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling

  Yusuke Yonamine, Kotaro Hiramatsu, Takuro Ideguchi, Takuro Ito, Tomomi Fujiwara, Yoshiko Miura, Keisuke Goda, Yu Hoshino

  RSC ADVANCES 10 (28) 16679 - 16686 2020/04 [Refereed][Not invited]
   

  Cellular metabolites are valuable in a diverse range of applications. For example, the unicellular green algaHaematococcus lacustrisproduces as a secondary metabolite the carotenoid pigment astaxanthin (AXT), which is widely used in nutraceutical, cosmetic, and food industries due to its strong antioxidant activity. In order to enhance the productivity ofH. lacustris, spatial and temporal understanding of its metabolic dynamics is essential. Here we show spatiotemporal monitoring of AXT production inH. lacustriscells by resonance Raman microscopy combined with stable isotope labeling. Specifically, we incorporated carbon dioxide ((CO2)-C-13) labeled with a stable isotope (C-13) intoH. lacustriscells through carbon fixation and traced its conversion to(13)C-AXT using our resonance Raman microscope. We incubatedH. lacustriscells under various conditions by switching, pulsing, and replacing(13)CO(2)and(12)CO(2). By measurement of these cells we determined the fixation time of(13)C-carbon, visualized the intracellular localization of(13)C- and(12)C-AXTs, and revealed the dynamic consumption-production equilibrium of the accumulated AXT. This work is a valuable step in the development of effective screening criteria for high AXT-producingH. lacustriscells.
• Homogeneous Oligomeric Ligands Prepared via Radical Polymerization that Recognize and Neutralize a Target Peptide

  Yu Hoshino, Shohei Taniguchi, Hinata Takimoto, Sotaro Akashi, Sho Katakami, Yusuke Yonamine, Yoshiko Miura

  Angewandte Chemie 132 (2) 689 - 693 2020/01/07
• Homogeneous Oligomeric Ligands Prepared via Radical Polymerization that Recognize and Neutralize a Target Peptide.

  Yu Hoshino, Shohei Taniguchi, Hinata Takimoto, Sotaro Akashi, Sho Katakami, Yusuke Yonamine, Yoshiko Miura

  Angewandte Chemie (International ed. in English) 59 (2) 679 - 683 2020/01/07 [Refereed][Not invited]
   

  Abiotic ligands that bind to specific biomolecules have attracted attention as substitutes for biomolecular ligands, such as antibodies and aptamers. Radical polymerization enables the production of robust polymeric ligands from inexpensive functional monomers. However, little has been reported about the production of monodispersed polymeric ligands. Herein, we present homogeneous ligands prepared via radical polymerization that recognize epitope sequences on a target peptide and neutralize the toxicity of the peptide. Taking advantage of controlled radical polymerization and separation, a library of multifunctional oligomers with discrete numbers of functional groups was prepared. Affinity screening revealed that the sequence specificity of the oligomer ligands strongly depended on the number of functional groups. The process reported here will become a general step for the development of abiotic ligands that recognize specific peptide sequences.
• Isolating Single Euglena gracilis Cells by Glass Microfluidics for Raman Analysis of Paramylon Biogenesis

  Ota Nobutoshi, Yonamine Yusuke, Asai Takuya, Yalikun Yaxiaer, Ito Takuro, Ozeki Yasuyuki, Hoshino Yu, Tanaka Yo

  ANALYTICAL CHEMISTRY 91 (15) 9631 - 9639 0003-2700 2019/08/06 [Refereed][Not invited]
   

  Time-course analysis of single cells is important to characterize heterogeneous activities of individual cells such as the metabolic response to their environment. Single-cell isolation is an essential step prior to time-course analysis of individual cells by collecting, culturing, and identifying multiple single-cell targets. Although single-cell isolation has been performed by various methods previously, a glass microfluidic device with semiclosed microchannels dramatically improved this process with its simple operation and easy transfer for time-course analysis of identified single cells. This study demonstrates isolating single cells of the highly motile microalgae, Euglena gracilis, by semiclosed microchannels with liquid flow only. The isolated single cells were identified in isolating channels and continuously cultured to track, by Raman microscopy, for the formation of subcellular granules composed of polysaccharide paramylon, a unique metabolite of E. gracilis, generated through photosynthesis. Through low-temperature glass bonding, a thin glass interface was incorporated to the microfluidic device. Thus, the device could perform the direct measurements of cultured single cells at high magnification by Raman microscopy with low background noise. In this study, the first demonstration of sequential monitoring of paramylon biogenesis in a single identified E. gracilis cell is shown.
• High-throughput label-free molecular fingerprinting flow cytometry

  Kotaro Hiramatsu, Takuro Ideguchi, Yusuke Yonamine, SangWook Lee, Yizhi Luo, Kazuki Hashimoto, Takuro Ito, Misa Hase, Jee-Woong Park, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yu Hoshino, Keisuke Goda

  SCIENCE ADVANCES 5 (1) 2375-2548 2019/01 [Refereed][Not invited]
   

  Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm(-1)) with a record--high throughput of similar to 2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics of Haematococcus lacustris.
• Monitoring Photosynthetic Activity in Microalgal Cells by Raman Spectroscopy with Deuterium Oxide as a Tracking Probe

  Yusuke Yonamine, Yuta Suzuki, Takuro Ito, Yoshiko Miura, Keisuke Goda, Yasuyuki Ozeki, Yu Hoshino

  CHEMBIOCHEM 18 (20) 2063 - 2068 1439-4227 2017/10 [Refereed][Not invited]
   

  Microalgae offer great potential for the production of biofuel, but high photosynthetic activity is demanded for the practical realisation of microalgal biofuels. To this end, it is essential to evaluate the photosynthetic activity of single microalgal cells in a heterogeneous population. In this study, we present a method to monitor the photosynthetic activity of microalgae (in particular Euglena gracilis, a microalgal species of unicellular, photosynthetic, flagellate protists as our model organism) at single-cell resolution by Raman spectroscopy with deuterium from deuterium oxide (D2O) as a tracking probe. Specifically, we replaced H2O in culture media with D2O up to a concentration of 20% without disturbing the growth rate of E.gracilis cells and evaluated C-D bond formation as a consequence of photosynthetic reactions by Raman spectroscopy. We used the probe to monitor the kinetics of the C-D bond formation in E.gracilis cells by incubating them in D2O media under light irradiation. Furthermore, we demonstrated Raman microscopy imaging of each single E.gracilis cell to discriminate deuterated cells from normal cells. Our results hold great promise for Raman-based screening of E.gracilis and potentially other microalgae with high photosynthetic activity by using D2O as a tracking probe.
• Conformation Manipulation and Motion of a Double Paddle Molecule on an Au(111) Surface

  We-Hyo Soe, Yasuhiro Shirai, Corentin Durand, Yusuke Yonamine, Kosuke Minami, Xavier Bouju, Marek Kolmer, Katsuhiko Ariga, Christian Joachim, Waka Nakanishi

  ACS NANO 11 (10) 10357 - 10365 1936-0851 2017/10 [Refereed][Not invited]
   

  The molecular conformation of a bisbinaphthyldurene (BBD) molecule is manipulated using a low-temperature ultrahigh-vacuum scanning tunneling microscope (LT-UHV STM) on an Au(111) surface. BBD has two binaphthyl groups at both ends connected to a central durene leading to anti/syn/flat conformers. In solution, dynamic nuclear magnetic resonance indicated the fast interexchange between the anti and syn conformers as confirmed by density functional theory calculations. After deposition in a submonolayer on an Au(111) surface, only the syn conformers were observed forming small islands of self-assembled syn dimers. The syn dimers can be separated into syn monomers by STM molecular manipulations. A flat conformer can also be prepared by using a peculiar mechanical unfolding of a syn monomer by STM manipulations. The experimental STM dI/dV and theoretical elastic scattering quantum chemistry maps of the low-lying tunneling resonances confirmed the flat conformer BBD molecule STM production. The key BBD electronic states for a step-by-step STM inelastic excitation lateral motion on the Au(111) are presented requiring no mechanical interactions between the STM tip apex and the BBD. On the BBD molecular board, selected STM tip apex positions for this inelastic tunneling excitation enable the flat BBD to move controllably on Au(111) by a step of 0.29 nm per bias voltage ramp.
• A polymer nanoparticle with engineered affinity for a vascular endothelial growth factor (VEGF(165))

  Hiroyuki Koide, Keiichi Yoshimatsu, Yu Hoshino, Shih-Hui Lee, Ai Okajima, Saki Ariizumi, Yudai Narita, Yusuke Yonamine, Adam C. Weisman, Yuri Nishimura, Naoto Oku, Yoshiko Miura, Kenneth J. Shea

  NATURE CHEMISTRY 9 (7) 715 - 722 1755-4330 2017/07 [Refereed][Not invited]
   

  Protein affinity reagents are widely used in basic research, diagnostics and separations and for clinical applications, the most common of which are antibodies. However, they often suffer from high cost, and difficulties in their development, production and storage. Here we show that a synthetic polymer nanoparticle (NP) can be engineered to have many of the functions of a protein affinity reagent. Polymer NPs with nM affinity to a key vascular endothelial growth factor (VEGF(165)) inhibit binding of the signalling protein to its receptor VEGFR-2, preventing receptor phosphorylation and downstream VEGF(165)-dependent endothelial cell migration and invasion into the extracellular matrix. In addition, the NPs inhibit VEGF-mediated new blood vessel formation in Matrigel plugs in vivo. Importantly, the non-toxic NPs were not found to exhibit off-target activity. These results support the assertion that synthetic polymers offer a new paradigm in the search for abiotic protein affinity reagents by providing many of the functions of their protein counterparts.
• pH-Responsive Cotton Effects in the d-d Transition Band of Self-Assembling Copper(II) Complexes with a Cholesteryl-Armed Ligand

  Takuya Sagami, Shota Umemoto, Yuhei O. Tahara, Makoto Miyata, Yusuke Yonamine, Daisuke Ishikawa, Taizo Mori, Katsuhiko Ariga, Hiroyuki Miyake, Satoshi Shinoda

  BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 90 (6) 739 - 745 0009-2673 2017/06 [Refereed][Not invited]
   

  New amphiphilic transition metal complexes with helical structures self-assembled to form disk-like units in 20wt% EtOH/water. d-d transition bands in the visible-near IR region were used to probe conformational changes during the assembly process. Self-assembly resulted in dynamic conformational changes and considerable enhancement of circular dichroism (CD) in the d-d transition bands due to steric interaction among neighboring complexes. A copper(II) complex showed the most dramatic CD changes upon self-assembly, including sign inversion of the CD signal. The self-assembled Cu(II) complexes also showed pH-responsive Cotton effects between pH 6 and pH 7 due to deprotonation. The Cotton effects of the Cu(II) complex could be finely tuned by varying the pH and ethanol content of the aqueous solvent.
• Mechanically Induced Opening-Closing Action of Binaphthyl Molecular Pliers: Digital Phase Transition versus Continuous Conformational Change

  Taizo Mori, Daisuke Ishikawa, Yusuke Yonamine, Yoshihisa Fujii, Jonathan P. Hill, Izumi Ichinose, Katsuhiko Ariga, Waka Nakanishi

  CHEMPHYSCHEM 18 (11) 1470 - 1474 1439-4235 2017/06 [Refereed][Not invited]
   

  Reversible dynamic control of structure is a significant challenge in molecular nanotechnology. Previously, we have reported a mechanically induced continuous (analog) conformational variation in an amphiphilic binaphthyl, where closing of molecular pliers was achieved by compression of a molecular monolayer composed of these molecules at the air-water interface. In this work we report that a phase transition induced by an applied mechanical stress enables discontinuous digital (1/0) opening of simple binaphthyl molecular pliers. A lipid matrix at the air-water interface promotes the formation of quasi-stable nanocrystals, in which binaphthyl molecules have an open transoid configuration. The crystallization/dissolution of quasi-stable binaphthyl crystals with accompanying conformational change is reversible and repeatable.
• Driving nanocars and nanomachines at interfaces: From concept of nanoarchitectonics to actual use in world wide race and hand operation

  Yasuhiro Shirai, Kosuke Minami, Waka Nakanishi, Yusuke Yonamine, Christian Joachim, Katsuhiko Ariga

  JAPANESE JOURNAL OF APPLIED PHYSICS 55 (11) 0021-4922 2016/11 [Refereed][Not invited]
   

  Nanomachine and molecular machines are state-of-the-art objects in current physics and chemistry. The operation and manufacturing of nanosize machines are top-level technologies that we have desired to accomplish for a long time. There have been extensive attempts to design and synthesize nanomachines. In this paper, we review the these attempts using the concept of nanoarchitectonics toward the design, synthesis, and testing of molecular machinery, especially at interfacial media. In the first half of this review, various historical attempts to design and prepare nanomachines are introduced as well as their operation mechanisms from their basic principles. Furthermore, in order to emphasize the importance and possibilities of this research field, we also give examples of two new challenging topics in the second half of this review: (i) a world wide nanocar race and (ii) new modes of nanomachine operation on water. The nanocar race event involves actual use of nanomachines and will take place in the near future, and nanomachine operation of a dynamic fluidic interface will enable future advances in nanomachine science and technology. (C) 2016 The Japan Society of Applied Physics
• Supramolecular 1-D polymerization of DNA origami through a dynamic process at the 2-dimensionally confined air-water interface

  Yusuke Yonamine, Keitel Cervantes-Salguero, Kosuke Minami, Ibuki Kawamata, Waka Nakanishi, Jonathan P. Hill, Satoshi Murata, Katsuhiko Ariga

  PHYSICAL CHEMISTRY CHEMICAL PHYSICS 18 (18) 12576 - 12581 1463-9076 2016/05 [Refereed][Not invited]
   

  In this study, a Langmuir-Blodgett (LB) system has been utilized for the regulation of polymerization of a DNA origami structure at the air-water interface as a two-dimensionally confined medium, which enables dynamic condensation of DNA origami units through variation of the film area at the macroscopic level (ca. 10-100 cm(2)). DNA origami sheets were conjugated with a cationic lipid (dioctadecyldimethylammonium bromide, 2C(18)N(+)) by electrostatic interaction and the corresponding LB-film was prepared. By applying dynamic pressure variation through compression-expansion processes, the lipid-modified DNA origami sheets underwent anisotropic polymerization forming a one-dimensionally assembled belt-shaped structure of a high aspect ratio although the thickness of the polymerized DNA origami was maintained at the unimolecular level. This approach opens up a new field of mechanical induction of the self-assembly of DNA origami structures.
• Mechanochemical Tuning of the Binaphthyl Conformation at the Air-Water Interface

  Daisuke Ishikawa, Taizo Mori, Yusuke Yonamine, Waka Nakanishi, David L. Cheung, Jonathan P. Hill, Katsuhiko Ariga

  ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54 (31) 8988 - 8991 1433-7851 2015/07 [Refereed][Not invited]
   

  Gradual and reversible tuning of the torsion angle of an amphiphilic chiral binaphthyl, from -90 degrees to -80 degrees, was achieved by application of a mechanical force to its molecular monolayer at the air-water interface. This 2D interface was an ideal location for mechanochemistry for molecular tuning and its experimental and theoretical analysis, since this lowered dimension enables high orientation of molecules and large variation in the area. A small mechanical energy (<1kcalmol(-1)) was applied to the monolayer, causing a large variation (>50%) in the area of the monolayer and modification of binaphthyl conformation. Single-molecule simulations revealed that mechanical energy was converted proportionally to torsional energy. Molecular dynamics simulations of the monolayer indicated that the global average torsion angle of a monolayer was gradually shifted.
• Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials

  Yu Hoshino, Yuka Arata, Yusuke Yonamine, Shih-Hui Lee, Aki Yamasaki, Ryousuke Tsuhara, Katsuhiko Yano, Kenneth J. Shea, Yoshiko Miura

  POLYMER JOURNAL 47 (2) 220 - 225 0032-3896 2015/02 [Refereed][Not invited]
   

  We describe the preparation and evaluation of nanogel-immobilized porous gel beads (GB) for application as a protein purification medium. Nanogel particles (NP) that bind with the Fc fragment of immunoglobulin G (IgG) were immobilized on the pore surface of macroporous hard GB containing quaternary ammonium cations on the surface via multipoint electrostatic interactions. The amount of NPs that were irreversibly immobilized in 1ml of GB slurry was determined to be similar to 30mg using fluorescent-labeled NPs. Images obtained via scanning electron microscopy established that the NPs were uniformly immobilized on the surface of the pores without blocking the macropores. The model target protein (IgG) was reversibly captured by the NP-immobilized GBs through NP-IgG interactions. NP-immobilized GBs have potential applications as novel affinity purification media for proteins, combining inexpensive and stable ligands with high-performance supports.
• Yusuke Yonamine, Keitel Cervantes-Salguero, Waka Nakanishi, Ibuki Kawamata, Kosuke Minami, Hirokazu Komatsu, Satoshi Murata, Jonathan P. Hillab, Katsuhiko Ariga.

  Yusuke Yonamine, Keitel Cervantes-Salguero, Waka Nakanishi, Ibuki Kawamata, Kosuke Minami, Hirokazu Komatsu, Satoshi Murata, Jonathan, P. Hillab, Katsuhiko Ariga

  Physical Chemistry Chemical Physics 17 32122 - 32125 2015 [Refereed][Not invited]
  
• Functional nanomaterials prepared by nanoarchitectonics-based supramolecular assembly

  Katsuhiko Ariga, Yusuke Yonamine, Jonathan P. Hill

  NATO Science for Peace and Security Series C: Environmental Security 139 45 - 61 1874-6519 2015 [Not refereed][Not invited]
   

  Recently, we have introduced the new concept of nanoarchitectonics to traditional supramolecular self-assembly to promote the development of the bottom-up approaches. The concept of nanoarchitectonics can be developed using synergic contributions of technical innovations such as atomic/molecular-level control, chemical nanofabrication, and self- and field-controlled organization. This concept might be applied to inorganic, organic, biochemical, and other systems, beyond the levels of substance, material, and system regardless of their dimensions i.e., macro, micro, or nano scale. In this chapter, recent examples of research on functional nanomaterials prepared by nanoarchitectonics-based supramolecular assembly are explained. These examples are classified according to their scale, (i) molecular-level nanoarchitectonics, (ii) microscale assembly, (iii) macroscopic materials with internal nanostructures.
• In situ 2D-extraction of DNA wheels by 3D through-solution transport

  Yusuke Yonamine, Keitel Cervantes-Salguero, Waka Nakanishi, Ibuki Kawamata, Kosuke Minami, Hirokazu Komatsu, Satoshi Murata, Jonathan P. Hill, Katsuhiko Ariga

  PHYSICAL CHEMISTRY CHEMICAL PHYSICS 17 (48) 32122 - 32125 1463-9076 2015 [Refereed][Not invited]
   

  Controlled transfer of DNA nanowheels from a hydrophilic to a hydrophobic surface was achieved by complexation of the nanowheels with a cationic lipid (2C(12)N(+)). 2D surface-assisted extraction, '2D-extraction', enabled structure-persistent transfer of DNA wheels, which could not be achieved by simple drop-casting.
• Research Update: Mesoporous sensor nanoarchitectonics

  Katsuhiko Ariga, Yusuke Yamauchi, Qingmin Ji, Yusuke Yonamine, Jonathan P. Hill

  APL MATERIALS 2 (3) 2166-532X 2014/03 [Refereed][Not invited]
   

  In this short review, we have selected three main subjects: (i) mesoporous materials, (ii) sensing applications, and (iii) the concept of nanoarchitectonics, as examples of recent hot topics in nanomaterials research. Mesoporous materials satisfy the conditions necessary not only for a wide range of applications but also for ease of production, by a variety of simple processes, which yield bulk quantities of materials without loss of their well-defined nanometric structural features. Sensing applications are of general importance because many events arise from interaction with external stimuli. In addition to these important features, nanoarchitectonics is a concept aimed at production of novel functionality of whole units according to concerted interactions within nanostructures. For the combined subject of mesoporous sensor nanoarchitectonics, we present recent examples of research in the corresponding fields categorized according to mechanism of detection including optical, electrical, and piezoelectric sensing. (C) 2014 Author(s).
• Layer-by-layer Nanoarchitectonics: Invention, Innovation, and Evolution

  Katsuhiko Ariga, Yusuke Yamauchi, Gaulthier Rydzek, Qingmin Ji, Yusuke Yonamine, Kevin C. -W. Wu, Jonathan P. Hill

  CHEMISTRY LETTERS 43 (1) 36 - 68 0366-7022 2014/01 [Refereed][Not invited]
   

  Materials fabrication with nanoscale structural precision based on bottom-up-type self-assembly has become more important in various current disciplines in chemistry including materials chemistry, organic chemistry, physical chemistry, analytical chemistry, biochemistry, colloid and surface chemistry, and supramolecular chemistry. Although the design of new materials based on nanoscale self-assembly is anticipated as a key concept, preparing complete three-dimensional structures at nanoscale precision remains a difficult target using current technologies. Rather, dimension-reduced approaches such as layering of two-dimensional nanostructures into precisely controlled lamellar nanomaterials are currently achievable. In particular, layer-by-layer (LbL) assembly is known as a highly versatile method for fabrication of controlled layered structures from various kinds of component materials using very simple, inexpensive, and rapid procedures. Therefore, fabrication of multilayer films through the LbL deposition process has attracted growing interest from various research communities. The high versatility and flexibility of LbL assembly is continuously creating new concepts, new materials, new procedures, and new applications. In this highlight review, we focus on nanoarchitectonics by LbL assembly. After an initial introduction on the invention and a brief history of the LbL assembly technique, innovations and the evolution of the technique are described based mainly on recent examples, which are categorized into two sections: (i) developments in methodology (technical, material, and phenomenological aspects with expansion of concept) and (ii) progress in applications (physical, chemical/biochemical, and biomedical applications).
• Nanoarchitectonics of Molecular Aggregates: Science and Technology

  Muruganathan Ramanathan, Kunlun Hong, Qingmin Ji, Yusuke Yonamine, Jonathan P. Hill, Katsuhiko Ariga

  JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 14 (1) 390 - 401 1533-4880 2014/01 [Refereed][Not invited]
   

  The field of making, studying and using molecular aggregates, in which the individual molecules (monomers) are arranged in a regular fashion, has come a long way. Taking control over the aggregation of small molecules and polymers in bulk, on surfaces and at interfaces pose a considerable challenge for their utilization in modern high tech applications. In this review, we provide a detailed insight into recent trends in molecular aggregates from the perspectives of nanoarchitectonics.
• Epitope Discovery for a Synthetic Polymer Nanoparticle: A New Strategy for Developing a Peptide Tag

  Keiichi Yoshimatsu, Tomohiko Yamazaki, Yu Hoshino, Paul E. Rose, Linda F. Epstein, Les P. Miranda, Philip Tagari, John M. Beierle, Yusuke Yonamine, Kenneth J. Shea

  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 136 (4) 1194 - 1197 0002-7863 2014/01 [Refereed][Not invited]
   

  We describe a novel epitope discovery strategy for creating an affinity agent/peptide tag pair. A synthetic polymer nanoparticle (NP) was used as the "bait" to catch an affinity peptide tag. Biotinylated peptide tag candidates of varied sequence and length were attached to an avidin platform and screened for affinity against the polymer NP. NP affinity for the avidin/peptide tag complexes was used to provide insight into factors that contribute NP/tag binding. The identified epitope sequence with an optimized length (tMel-tag) was fused to two recombinant proteins. The tagged proteins exhibited higher NP affinity than proteins without tags. The results establish that a fusion peptide tag consisting of optimized 15 amino acid residues can provide strong affinity to an abiotic polymer NP. The affinity and selectivity of NP/tMel-tag interactions were exploited for protein purification in conjunction with immobilized metal ion/His6-tag interactions to prepare highly purified recombinant proteins. This strategy makes available inexpensive, abiotic synthetic polymers as affinity agents for peptide tags and provides alternatives for important applications where more costly affinity agents are used.
• Polymer Nanoparticle-Protein Interface. Evaluation of the Contribution of Positively Charged Functional Groups to Protein Affinity

  Yusuke Yonamine, Keiichi Yoshimatsu, Shih-Hui Lee, Yu Hoshino, Yoshio Okahata, Kenneth J. Shea

  ACS APPLIED MATERIALS & INTERFACES 5 (2) 374 - 379 1944-8244 2013/01 [Refereed][Not invited]
   

  Cationic-functionalized polymer nanoparticles (NPs) show strikingly distinct affinities to proteins depending on the nature of the cationic functional group. N-Isopropylacrylamide (NIPAm) polymer NPs incorporating three types of positively charged functional groups (guanidinium, primary amino, and quaternary ammonium groups) were prepared by precipitation polymerization. The affinities to fibrinogen, a protein with an isoelectric point (pI) of 5.5, were compared using UV-vis spectrometry and a quartz crystal microbalance (QCM). Guanidinium-containing NPs showed the highest affinity to fibrinogen. The observation is attributed to strong, specific interactions with carboxylate groups on the protein surface. The affinity of the positively charged NPs to proteins with a range of pis revealed that protein-NP affinity is due to a combination of ionic, hydrogen bonding, and hydrophobic interactions. Protein affinity can be modulated by varying the composition of these functional monomers in the acrylamide NPs. Engineered NPs containing the guanidinium group with hydrophobic and hydrogen bonding functional groups were used in an affinity precipitation for the selective separation of fibrinogen from a plasma protein mixture. Circular dichroism (CD) revealed that the protein was not denatured in the process of binding or release.
• ELISA-Mimic Screen for Synthetic Polymer Nanoparticles with High Affinity to Target Proteins

  Yusuke Yonamine, Yu Hoshino, Kenneth J. Shea

  BIOMACROMOLECULES 13 (9) 2952 - 2957 1525-7797 2012/09 [Refereed][Not invited]
   

  Synthetic polymer nanoparticles (NPs) that display high affinity to protein targets have significant potential for medical and biotechnological applications as protein capture agents or functional replacements of antibodies ("plastic antibodies"). In this study, we modified an immunological assay (enzyme-linked immunosorbent assay: ELISA.) into a high-throughput screening method to select nanoparticles with high affinity to target proteins. Histone and fibrinogen were chosen as target proteins to demonstrate this concept. The selection process utilized a biotinylated NP library constructed with combinations of functional monomers. The screen identified NPs with distinctive functional group compositions that exhibited high affinity to either histone or fibrinogen. The variation of protein affinity with changes in the nature and amount of functional groups in the NP provided chemical insight into the principle determinants of protein-NP binding. The NP affinity was semiquantified using the ELISA-mimic assay by varying the NP concentrations. The screening results were found to correlate with solution-based assay results. This screening system utilizing a biotinylated NP is a general approach to optimize functional monomer compositions and can be used to rapidly search for synthetic polymers with high (or low) affinity for target biological macromolecules.
• Light-Triggered Charge Reversal of Organic-Silica Hybrid Nanoparticles

  Li-Chih Hu, Yusuke Yonamine, Shih-Hui Lee, Wytze E. van der Veer, Kenneth J. Shea

  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 134 (27) 11072 - 11075 0002-7863 2012/07 [Refereed][Not invited]
   

  A functional nanoparticle with light-triggered charge reversal based on a protected amine-bridged polysilsesquioxane was designed. An emulsion- and amine-free sol gel synthesis was developed to prepare uniform nanospheres. Photolysis of suspensions of these nanoparticles results in a reversal of the zeta potential. This behavior has been used to trigger nanoparticle self-assembly, nanocomposite hydrogel formation, and nanoparticle release, showing the potential of this material in nanoscale manipulation and nanoparticle therapy.
• Temperature-Responsive "Catch and Release" of Proteins by using Multifunctional Polymer-Based Nanoparticles

  Keiichi Yoshimatsu, Benjamin K. Lesel, Yusuke Yonamine, John M. Beierle, Yu Hoshino, Kenneth J. Shea

  ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 51 (10) 2405 - 2408 1433-7851 2012 [Refereed][Not invited]
  
• Covalent Modification of DNA with Azetidinium Lipids

  Yusuke Yonamine, Takayoshi Kawasaki, Yoshio Okahata

  CHEMISTRY LETTERS 39 (2) 84 - 85 0366-7022 2010/02 [Refereed][Not invited]
   

  We Studied covalent bond formation of an ion complex of phosphate and azetidinium and applied the reaction to preparation of DNA which was elaborated at the phosphate units with azetidinium lipids.
• Photochromism and chirality propagation of chromene amphiphile aggregates in aqueous ethanol solutions

  Takayoshi Kawasaki, Sayaka Horie, Kentarou Asaoka, Yuichi Manaka, Yusuke Yonamine, Yoshio Okahata

  CHEMISTRY LETTERS 36 (8) 1048 - 1049 0366-7022 2007/08 [Refereed][Not invited]
   

  Spectroscopic and morphologic characteristics of an racemic chromene cationic amphiphile 1(+) having a chiral camphor sulfonate counter anion ((+) or (-) CS*-) were studied in aggregates in aqueous ethanol solutions. The media-dependent photo-chromism of 1(+)/CS*- and the enantio-preferential selective ring-closing process after UV irradiation were observed owing to the propagation of the chirality from the counter anion CS*-.

MISC

• Monitoring photosynthetic metabolism in a microalgal cell by Raman spectroscopy with stable isotope labeling

  Yusuke Yonamine, Yuta Suzuki, Takuro Ito, Yoshiko Miura, Yasuyuki Ozeki, Keisuke Goda, Yu Hoshino  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY  255-  2018/03
• 気液界面における共役分子の構造・物性制御

  中西和嘉, 石川大輔, 与那嶺雄介, 森泰蔵, 榊原圭太, HILL Jonathan P, 有賀克彦  基礎有機化学討論会要旨集  25th-  38  2014/08/25  [Not refereed][Not invited]
  
• Design of polymer nanoparticles that are capable of neutralizing toxicity of fetal proteins

  Yu Hoshino, Hiroyuki Koide, Dai Oyama, Yusuke Yonamine, Shih-Hui Lee, Naoto Oku, Kenneth J. Shea  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY  241-  2011/03  [Not refereed][Not invited]
  

Research Grants & Projects

• 藻類色素に安定同位体を導入して多色化した高感度ラマン検出剤の開発

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 2023/04 -2026/03 

  Author : 与那嶺 雄介
• 合成高分子アシストによる機能強化タンパク質の開発

  独立行政法人日本学術振興会：科学研究費補助金 基盤研究(C)

  Date (from‐to) : 2018/04 -2021/03 

  Author : 与那嶺 雄介
• 界面での力学刺激によるキラリティーの誘起

  独立行政法人日本学術振興会：科学研究費補助金 若⼿研究(B)

  Date (from‐to) : 2014/04 -2016/03 

  Author : 与那嶺 雄介

Educational Activities

Teaching Experience