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Last Updated :2024/07/25

研究者情報

マスター

アカウント（マスター）

  • 氏名

    神　繁樹(ジン　シゲキ), ジン　シゲキ

所属（マスター）

  • 医学研究院　社会医学系部門　社会医学分野

所属（マスター）

  • 医学研究院　社会医学系部門　社会医学分野

researchmap

プロフィール情報

学位

  • 博士（農学）（岩手大学）

プロフィール情報

  • 姓

    神

  • 名

    繁樹

  • ID各種

    201301001564837994

業績リスト

研究分野

  • ライフサイエンス / 法医学

論文

  • Shogo Shimbashi, Akiko Takeuchi, Motoo Yoshimiya, Shigeki Jin, Kotaro Matoba, Hideki Hyodoh
    Legal Medicine 69 102448 - 102448 2024年07月
  • Akiko Takeuchi, Shigeki Jin, Manabu Murakami, Kotaro Matoba
    Pakistan journal of medical sciences 40 6 1313 - 1314 2024年07月
  • Manabu Murakami, Akiko Takeuchi, Shigeki Jin, Kotaro Matoba
    Journal of the Chinese Medical Association : JCMA 86 11 1037 - 1038 2023年11月01日
  • Manabu Murakami, Akiko Takeuchi, Shigeki Jin, Kotaro Matoba
    Acta medica portuguesa 36 9 613 - 614 2023年09月01日
  • db/dbマウス副腎におけるステロイドホルモン合成亢進機序DHCR24阻害薬の効果
    上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌 99 1 392 - 392 (一社)日本内分泌学会 2023年05月
  • db/dbマウスにおける副腎ステロイドホルモン合成亢進に対するDHCR24阻害薬の効果と副腎内代謝変化の検討
    上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本臨床分子医学会学術総会プログラム・抄録集 58回 59 - 59 日本臨床分子医学会 2023年04月
  • Akiko Takeuchi, Hideki Hyodoh, Shigeki Jin, Satoshi Tanaka, Manabu Murakami, Kazuyuki Minowa, Kotaro Matoba
    Current medical imaging 2023年03月28日 
    BACKGROUND: Obstructive sleep apnea syndrome (OSAS) can cause sudden death during sleep. Previous findings have suggested that OSAS development is related to maxillofacial morphology. Evaluation of facial morphology can determine the risk of developing the disease, and establishing an objective method to assess the underlying etiology of OSAS-related death would be advantageous. OBJECTIVE: The objective of this study is to determine the key features of obstructive sleep apnea syndrome (OSAS) using postmortem oral and pharyngeal computed tomography (CT). METHODS: We retrospectively assessed autopsy cases of patients with (n=25) and without (n=25) OSAS-related death. We used oral and pharyngeal CT images to compare the oral and pharyngeal cavity volume (OPCV), oral and pharyngeal soft tissue volume (OPSV), oral and pharyngeal air space volume (OPAV), and OPAV to OPCV ratio (%air). Receiver operating curve (ROC) analysis was used to determine the accuracy of OSAS prediction. We assessed participants with body mass index (BMI) values within the normal range. RESULTS: Among the 50 subjects, we observed significant between-group differences in OPSV, OPAV, and % air, whereas there were significant between-group differences in OPSV and %air among 28 subjects with normal BMI values. Both comparisons suggested that OSAS-related death was associated with low %air and high OPSV values. CONCLUSION: The %air and OPSV are useful for assessing postmortem oropharyngeal CT images. OSAS-related sudden death is likely when %air and OPSV values are ≤20.1% and ≥127.2 ml, respectively. Among those with normal BMI values, % air and OPSV values of ≤22.8% and ≥111.5 ml, respectively, predict OSAS-related sudden death.
  • 上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌 98 5 1443 - 1443 (一社)日本内分泌学会 2023年03月
  • Shogo Shimbashi, Rina Hayata, Kotaro Matoba, Atsuko Saito, Tomoko Matoba, Akiko Takeuchi, Shigeki Jin, Hideki Hyodoh
    Legal medicine (Tokyo, Japan) 60 102178 - 102178 2023年02月 
    PURPOSE: The purpose of this study was to review the findings of computed tomography (CT) performed early postmortem on infants and to clarify the postmortem CT lung findings that occur in the absence of abnormal histopathological findings. MATERIALS AND METHODS: From July 2016 to March 2022, 72 infants were autopsied with postmortem CT (41 boys 31 girls, aged 0-36 (mean 8.2) months). Autopsy and postmortem CT lung findings were compared with the causes of death identified by the autopsies, namely sudden infant death syndrome (n = 37), acute circulatory system disease (18), drowning (7), asphyxia (5), and dehydration/undernutrition (5). RESULTS: The %aerated lung volume (-700 HU or less) ranged from 0 % to 33 % (mean 1.5 %, median 0 %), being <1 % in 61 cases (84.7 %) and >3 % in 3/5 (60 %) of the dehydration/undernutrition group. The dehydration/undernutrition group showed significant preservation of lung field air content compared with the other causes of death groups (p < 0.05). Receiver characteristic curve analysis showed a cut off value of 0.8 % and area under the curve of 0.88806. The drowning group had significantly greater pleural cavity fluid retention than the other causes of death groups (p < 0.05). No correlation was found between postmortem interval and pleural cavity fluid retention. However, resuscitation time and pleural cavity fluid retention were correlated. CONCLUSION: Evaluation of CT values on postmortem lung fields of infants usually reveals a marked decrease in air content. When air content exceeds 0.8% on infant postmortem CT, dehydration/undernutrition should be considered in the differential diagnosis.
  • Manabu Murakami, Akiko Takeuchi, Shigeki Jin, Kotaro Matoba
    Pakistan journal of medical sciences 39 4 1219 - 1220 2023年
  • Ryohei Fujita, Shigeki Jin, Kotaro Matoba, Yoichiro Hoshino
    Scientia Horticulturae 308 111547 - 111547 2023年01月 [査読有り]
  • 直腸穿孔の原因特定に死後CT検査が有用であった一剖検例
    的場 光太郎, 神 繁樹, 齋藤 厚子, 的場 智子, 竹内 明子, 赤川 有希, 早田 璃菜, 真橋 尚吾, 兵頭 秀樹
    法医学の実際と研究 65 119 - 123 法医学談話会 2022年11月
  • 直腸穿孔の原因特定に死後CT検査が有用であった一剖検例
    的場 光太郎, 神 繁樹, 齋藤 厚子, 的場 智子, 竹内 明子, 赤川 有希, 早田 璃菜, 真橋 尚吾, 兵頭 秀樹
    法医学の実際と研究 65 119 - 123 法医学談話会 2022年11月
  • メトホルミン中毒による重篤な乳酸アシドーシスに対して血液浄化療法を行い救命に成功した一例
    江原 慶介, 大城 あき子, 俵 敏弘, 岡本 博之, 清水 隆文, 大西 新介, 森下 由香, 奈良 理, 早川 峰司, 神 繁樹, 的場 光太郎
    日本救急医学会雑誌 33 10 793 - 793 (一社)日本救急医学会 2022年10月
  • 神 繁樹, 的場 光太郎, 赤川 有希, 早川 璃菜, 魚住 諒
    JSBMS Letters 47 Suppl. 78 - 78 (一社)日本医用マススペクトル学会 2022年08月
  • Manabu Murakami, Shigeki Jin, Akiko Takeuchi, Kotaro Matoba
    Pakistan Journal of Medical Sciences 38 6 1724 - 1725 2022年06月27日 [査読有り]
     
    doi: https://doi.org/10.12669/pjms.38.6.6058 How to cite this:Murakami M, Jin S, Takeuchi A, Matoba K. Nonverbal communication skills: New-era education needs of younger generation medical students. Pak J Med Sci. 2022;38(6):---------. doi: https://doi.org/10.12669/pjms.38.6.6058 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Hideki Hyodoh, Akiko Takeuchi, Kotaro Matoba, Manabu Murakami, Tomoko Matoba, Atsuko Saito, Shigeki Jin
    Legal Medicine 56 102048 - 102048 2022年05月
  • Kotaro Matoba, Manabu Murakami, Emi Fujita, Shigeki Jin, Ryosuke Ogasawara, Tomoko Matoba, Akiko Takeuchi, Sanae Haga, Michitaka Ozaki, Hideki Hyodoh
    Legal medicine (Tokyo, Japan) 57 102071 - 102071 2022年04月15日 
    In forensic medicine, although various alcohols have been reported as indicators of decomposition in collected blood, no studies have examined short-chain fatty acids as indicators. In this study, the blood n-butyric acid concentration was quantified, and the association between n-butyric acid and decomposition was investigated to determine whether the detection of n-butyric acid could be a new indicator of decomposition. Among the forensic autopsies performed from 2016 to 2018 in our laboratory, the cases were divided into decomposed (n = 20) and non-decomposed (n = 20) groups based on macroscopic findings. Blood samples collected at the time of autopsy were derivatized with 3-nitrophenylhydrazine hydrochloride after solid-phase extraction. The n-butyric acid concentration was measured using liquid chromatography-tandem mass spectrometry. In addition, ethanol and n-propanol were measured using a gas chromatography-flame ionization detector. There was a significant difference (p < 0.01) in the concentrations of n-butyric acid between the decomposed and non-decomposed groups (0.343 ± 0.259 [0.030-0.973] and 0.003 ± 0.002 [0.001-0.007] mg/mL, respectively). In the decomposed group, n-butyric acid was detected at high concentrations, even in cases where n-propanol was low. These results suggest that n-butyric acid is more likely to be an indicator of blood decomposition than n-propanol.
  • Manabu Murakami, Shigeki Jin, Akiko Takeuchi, Kotaro Matoba
    Acta Médica Portuguesa 35 3 231 - 231 2022年03月02日 
    N/a.
  • Nobuyuki Hayashi, Tomomi Ujihara, Shigeki Jin
    The Analyst 147 20 4480 - 4488 2022年 [査読有り]
     
    It is possible to estimate astringent intensities of catechin solutions based on changes in the intensity of fluorescence emission.
  • 3-クロロチロシンの検出によって塩素ガス中毒死を診断した一剖検例
    的場 光太郎, 神 繁樹, 齋藤 厚子, 的場 智子, 竹内 明子, 赤川 有希, 早田 璃菜, 兵頭 秀樹
    法医学の実際と研究 64 25 - 29 法医学談話会 2021年11月
  • S. Haga, A. Kanno, N. Morita, S. Jin, K. Matoba, T. Ozawa, M. Ozaki
    The Journal of Surgical Research 270 124 - 138 2021年10月 [査読有り]
  • 千葉 幸輝, 亀田 啓, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌 97 1 225 - 225 (一社)日本内分泌学会 2021年04月
  • クロバエ科の蛆が薬物検査に有用であった一剖検例
    的場 光太郎, 奥谷 菜穂子, 神 繁樹, 齋藤 厚子, 的場 智子, 竹内 明子, 兵頭 秀樹
    法医学の実際と研究 63 103 - 106 法医学談話会 2020年11月 
    症例は60歳代女性で、無職、独居、うつ病の既往、薬物多量服薬の自殺企図歴あり。自宅内に多数のハエが飛んでいると近所の住民から通報があり、警察官が自宅内を確認したところ寝室の床上に左横臥で倒れていた。高度腐敗状態であり、現場で死亡が確認された。警察等が取り扱う死体の死因または身元の調査等に関する法律に基づく調査法解剖が実施された。脾臓を圧迫して採取した血液様液体をQuEChERS法によって前処理を行い、液体クロマトグラフィー質量分析によって分析したところ、エスタゾラム、ブロマゼパム、スルピリド、クエチアピンが高濃度で検出された。検出された薬物の種類は現場にあった空薬包の処方薬と一致しており、処方薬を多量に服用して死亡したものと考えられた。死体を蚕食している蛆から薬物を検出することは可能であり、法医診断に有用であると考えられた。
  • Shigeki Jin, Manabu Murakami, Kotaro Matoba, Tomoko Matoba, Sanae Haga, Michitaka Ozaki, Akiko Takeuchi, Hideki Hyodoh
    Legal Medicine 47 101765 - 101765 2020年11月 [査読有り]
     
    Quantitative analysis of thiosulfate is useful for diagnosing hydrogen sulfide poisoning. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables more rapid and sensitive measurements than previous methodologies. As simple measurements of blood thiosulfate concentration are affected by the blood matrix, blood is used as the solvent to prepare the standard solution for calibration curve generation. Thus, a large amount of blood devoid of thiosulfate is required. We developed a preparation method by incorporating an ultrafiltration step to overcome this limitation and generate a calibration curve using a standard solution prepared with pure water. We used this improved method to investigate the stability of thiosulfate in refrigerated samples. To compare the effects of refrigeration, blood samples were prepared using the following two methods: one sample was treated with a 50-kDa exclusion ultrafiltration membrane and the other was not treated. The samples were stored at 4 °C, and then measured at 0, 3, 6, 24, 48, and 96 h. The incorporation of the ultrafiltration step in the measurement procedure enabled the quantification of thiosulfate, by plotting a calibration curve using a standard of pure water; it did not require a blood standard. Additionally, the reduction in whole blood thiosulfate concentration was within 10% during 2 days of refrigeration. Thus, the need for a large amount of blood to prepare the standard solution was resolved by the ultrafiltration step in test sample preparation. This method is useful to measure thiosulfate concentration and is not hindered by sample refrigeration for a few days.
  • Ryohei Fujita, Takahiro Hayasaka, Shigeki Jin, Shu-Ping Hui, Yoichiro Hoshino
    Plant Science 300 110633 - 110633 2020年11月 [査読有り]
     
    Haskap (Lonicera caerulea subsp. edulis), a shrub with violet-blue fruits, is distributed mainly in Hokkaido, Japan. Miyama-uguisukagura (Lonicera gracilipes), a species related to Haskap, produces red fruits. Interspecific hybridization of Miyama-uguisukagura and Haskap was performed to introduce novel characteristics in the resulting hybrids. The shape and color of the interspecific hybrid fruits differed from those of the parent fruits. A comparison of anthocyanin distribution among these three fruit types by imaging mass spectrometry (IMS) revealed the presence of five different anthocyanins. The average cyanidin 3,5-diglucoside and peonidin 3,5-diglucoside intensities in the interspecific hybrid fruit were higher than those of the parent fruits, whereas the average pelargonidin 3-glucoside, cyanidin 3-glucoside, and peonidin 3-glucoside intensities were the highest in Haskap. All anthocyanins were mainly accumulated in the inner and outer skins of Haskap and interspecific hybrid fruits, and in the skin of Miyama-uguisukagura fruits. The order of signal intensities of all anthocyanins among the three fruits was unchanged in different regions. Additionally, a comparison of IMS and LC/MS data from our previous study confirmed the possibility of comparing multiple fruits in the same plate by IMS. Thus, we elucidated anthocyanin distribution patterns of the interspecific hybrid and parent fruits by IMS.
  • 血中チオ硫酸塩濃度の保存による変化について
    神 繁樹, 的場 光太郎, 的場 智子, 兵頭 秀樹
    日本法医学雑誌 74 1 73 - 73 (NPO)日本法医学会 2020年08月
  • クロバエ科の蛆が薬物検査に有用であった一剖検例
    的場 光太郎, 奥谷 菜穂子, 神 繁樹, 兵頭 秀樹
    日本法医学雑誌 74 1 90 - 90 (NPO)日本法医学会 2020年08月
  • 千葉 幸輝, 亀田 啓, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌 96 1 366 - 366 (一社)日本内分泌学会 2020年08月
  • Tsuyoshi Murai, Shigeki Jin, Mari Itoh, Yukiko Horie, Tatsuya Higashi, Shigeo Ikegawa
    Steroids 158 108605 - 108605 2020年06月 [査読有り]
     
    Steryl glucosides (SGs) and acylated steryl glucosides (ASGs) are phytochemicals found in plant-based foods and are known as bioactive compounds with potential health benefits. These include anti-inflammatory properties, anti-diabetic effects, and modulation of immunoregulatory functions as well as having cholesterol lowering effects. In this study, three major SGs, i.e., glucosides of β-sitosterol, stigmasterol, and campesterol, were synthesized and used as standards for measurement of their contents in rice bran (RB)-based fermented food (FBRA) utilizing Aspergillus oryzae and raw material (RM). The compounds were quantified using liquid chromatography/electrospray ionization-tandem mass spectrometry. It was found that β-sitosteryl glucoside was most abundant among the analyzed glucosides in both samples, and the contents of each SG in FBRA decreased about 35% from those of RM. In contrast to SGs, the contents of ASGs in FBRA increased 1.5-fold during the fermentation process as evidenced by an alkaline hydrolysis. The present results suggest that the FBRA might have greater beneficial effects than the RM, since ASGs have shown to have more potent cholesterol lowering effects and stronger anti-diabetic properties than SGs.
  • Casey Smith, Kotaro Matoba, Hideki Hyodoh, Manabu Murakami, Atsuko Saito, Tomoko Matoba, Nahoko Okuya, Shigeki Jin
    Legal medicine (Tokyo, Japan) 44 101694 - 101694 2020年03月19日 [査読有り][通常論文]
     
    Ground glass attenuation (GGA) of the lungs is a common finding of post-mortem computed tomography (PMCT) that is associated with pulmonary congestion and edema due to cardiac failure during the agonal period, or due to post-mortem hypostasis. However, hypo-attenuation of the lung is an atypical finding of PMCT, and is usually a consequence of hypovolemic states before death or postmortem body posture after death. Previous studies have shown a few differential diagnoses, such as hypothermia, massive hemorrhage, asphyxiation by hanging, and dehydration, for hypo-attenuation of the lung. This report presents the case of a woman who died suddenly because of an asthma attack. Our PMCT analysis demonstrated hypoattenuation of the lung in this case. We suggest fatal asthma as a differential diagnosis for the appearance of hypo-attenuation of lungs on PMCT.
  • Hideki Hyodoh, Kotaro Matoba, Manabu Murakami, Atsuko Saito, Nahoko Okuya, Tomoko Matoba, Shigeki Jin
    Forensic Imaging 20 200367 - 200367 2020年03月 [査読有り]
  • Ryohei Fujita, Shigeki Jin, Takahiro Hayasaka, Kotaro Matoba, Yoichiro Hoshino
    The Horticulture Journal 89 4 343 - 350 2020年 [査読有り][通常論文]
  • Sanae Haga, YiMin, Hikari Yamaki, Shigeki Jin, Tetsuya Sogon, Naoki Morita, Michitaka Ozaki
    Bioscience, Biotechnology, and Biochemistry 83 11 2110 - 2120 2019年11月02日 [査読有り]
     
    ABSTRACT Bilberry has been reported to have anti-oxidant and anti-inflammatory properties. We studied the effect of bilberry (Vaccinium myrtillus L.) fruits extracts (BEs) on the pathogenesis caused by lipid accumulation in fatty liver and non-alcoholic steatohepatitis (NASH). 5 μg/ml of BEs was enough to suppress lipid accumulation in the fatty liver model of the mouse hepatic AML12 cells. BEs increased cell viability and anti-oxidant capacity, presumably by activating (phosphorylating) Akt/STAT3 and inducing MnSOD/catalase. BEs also significantly reduced Rubicon and induced p62/SQSTM1, possibly contributing to reduce cellular lipids (lipophagy). When the mice were fed supplemented with BEs (5% or 10%, w/w), hepatic steatosis, injury, and hypercholesterolemia/hyperglycemia were significantly improved. Furthermore, histological and cytokine studies indicated that BEs possibly suppress hepatic inflammation (hepatitis) and fibrosis. Therefore, BEs improved liver steatosis and injury, and potentially suppress fibrosis by suppressing inflammatory response, which therefore may prevent the progression of fatty liver to NASH.
  • 溺死事例の死戦期に起きた食物誤嚥が解剖所見や検査結果に与える影響に関する検討
    的場 光太郎, 兵頭 秀樹, 齋藤 厚子, 的場 智子, 奥谷 菜穂子, 竹内 明子, 邵 旻蓉, 神 繁樹
    法医学の実際と研究 62 15 - 19 法医学談話会 2019年11月 
    20歳代男性2名が海水浴中に行方不明となり、翌日、付近の海底において2名とも発見され、死亡が確認された。海水浴中に離岸流によって沖に流されたことが事故の原因である可能性が高いが、発見から約1日後に2名とも司法解剖となった。事例1では、肺は膨隆して左右肺の内側縁が前縦隔において重なっており、パルタウフ斑を認めた。肺重量は左675g、右725g、心重量は296g、左胸水450mL、右胸水460mL、脾臓重量は85g、胃内容は食物650mL、心臓内に360mLの軟凝血を少量に含む暗赤色流動血、気道内に食物片は認めず、気管内に白色細小泡沫を認めた。事例2では、肺は膨隆して左右肺の内側縁が前縦隔において近接しており、パルタウフ斑を認めた。肺重量は左426g、右567g、心重量は264g、左胸水170mL、右胸水130mL、脾臓重量は61g、胃内容は食物750mL、心臓内に280mLの軟凝血を微量に含む暗赤色流動血、咽頭から喉頭に食物片を多量に認め、気管〜気管支に多量の食物を認めた。
  • 死後造影CTに関する検討(Postmortem enhanced CT(PMeCT)
    兵頭 秀樹, 的場 光太郎, 神 繁樹
    日本法医学雑誌 73 1 73 - 73 (NPO)日本法医学会 2019年05月
  • 死後変化による腐敗が血液中のチオ硫酸濃度に与える影響について
    的場 光太郎, 神 繁樹, 兵頭 秀樹
    日本法医学雑誌 73 1 85 - 85 (NPO)日本法医学会 2019年05月
  • チオ硫酸塩測定における血液サンプルの前処理の検討
    神 繁樹, 的場 光太郎, 兵頭 秀樹
    日本法医学雑誌 73 1 86 - 86 (NPO)日本法医学会 2019年05月
  • 心筋トロポニンT濃度の死後変化の影響に関する検討
    的場 光太郎, 兵頭 秀樹, 齋藤 厚子, 石田 理沙, 神 繁樹
    法医学の実際と研究 61 151 - 154 法医学談話会 2018年11月
  • Kotaro Matoba, Hideki Hyodoh, Lisa Ishida, Manabu Murakami, Tomoko Matoba, Atsuko Saito, Nahoko Okuya, Sahar Almansoori, Emi Fujita, Mayumi Yamase, Minyo Shao, Shigeki Jin
    Legal Medicine 35 61 - 65 2018年11月 [査読有り]
  • Photo-Activatable Akt Probe: A New Tool to Study the Akt-Dependent Physiopathology of Cancer Cells.
    尾崎 倫孝
    Oncology Research 26 6 467 - 472 2018年 [査読有り][通常論文]
  • 溺水時の死戦期の食物誤嚥が諸検査に与える影響に関する考察
    的場 光太郎, 兵頭 秀樹, 馮 飛, 神 繁樹
    法医学の実際と研究 60 262 - 263 法医学談話会 2017年11月
  • Kotaro Matoba, Hideki Hyodoh, Manabu Murakami, Atsuko Saito, Tomoko Matoba, Lisa Ishida, Emi Fujita, Mayumi Yamase, Shigeki Jin
    Legal Medicine 29 77 - 81 2017年11月 [査読有り]
  • 腐敗脳に対する凍結処置による肉眼的観察の実験的検討
    兵頭 秀樹, 的場 光太郎, 村上 学, 的場 智子, 齊藤 厚子, 馮 飛, 神 繁樹
    日本法医学雑誌 71 1 73 - 73 (NPO)日本法医学会 2017年05月
  • 法医解剖における腐敗した脳の肉眼的観察のための凍結処理法の開発
    的場 光太郎, 兵頭 秀樹, 村上 学, 的場 智子, 齋藤 厚子, 馮 飛, 神 繁樹
    日本法医学雑誌 71 1 73 - 73 (NPO)日本法医学会 2017年05月
  • Matoba Kotaro, Hyodoh Hideki, Murakami Manabu, Matoba Tomoko, Saito Atsuko, Feng Fei, Jin Shigeki
    Legal medicine 26 6 - 10 Elsevier 2017年05月 [査読有り]
     
    Purpose: To evaluate the usefulness of the applied freezing technique in putrefied brain for macroscopic investigation. Materials and methods: From October 2015 to September 2016, first the brains of 10 cadavers (control group: male 6, female 4, age 20-80 (mean 61.5), postmortem intervals (PMI) 14-75 (mean 29.7) days) were inspected following the standard practice (without freezing preparation), and then with 10 cadavers (freezing group: male 7, female 3, age 41-88 (mean 60.4), PMI 7-75 (mean 29.2) days) the freezing technique was used before the autopsy. The cut brain was investigated, and the gray-white matter difference was evaluated macroscopically. Results: In the control group, the brain parenchyma leaked out like sludge in 5, and there was difficulty maintaining its structure in 7. The gray-white matter difference was well visible in 3, but hard to distinguish in 3, and the total scores ranged from 0 to 9 (mean 4.4) points. In the freezing group, the entire putrefied brain was extracted as a solid organ, the gray-white matter differences were well visible, and the total scores were 6.7-9 (8.3) points. The gray-white matter difference was preserved in the freezing group (p < 0.05). Conclusion: The freezing procedures to evaluate the putrefied brain have been successfully applied, and it could be statistically more useful in putrefied brain investigation than the ordinary procedure. Postmortem CT can be useful to evaluate not only the degree of brain putrefaction, but also the degree of brain parenchyma freezing.
  • Hideki Hyodoh, Kotaro Matoba, Manabu Murakami, Tomoko Matoba, Atsuko Saito, Fei Feng, Shigeki Jin
    Legal Medicine 24 19 - 23 2017年01月 [査読有り]
  • Chikatoshi Maseda, Akira Hayakawa, Katsuhiro Okuda, Masaru Asari, Hiroki Tanaka, Hiromi Yamada, Shigeki Jin, Kie Horioka, Kotaro Matoba, Hiroshi Shiono, Kazuo Matsubara, Keiko Shimizu
    Legal Medicine 24 67 - 74 2017年01月 [査読有り]
     
    Being a stable metabolite of hydrogen sulfide, thiosulfate has been utilized as an index for hydrogen sulfide poisoning (HSP). Thiosulfate analysis is mainly performed using gas chromatography/mass spectrometry (GC-MS) due to its high sensitivity and specificity. The GC-MS analysis requires two-step derivatizations of thiosulfate, and the derivative is not stable in solution as it has a disulfide moiety. To resolve this stability issue, we developed a novel analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for monitoring the pentafluorobenzyl derivative of thiosulfate (the first reaction product of the GC-MS method) in this study. The established method exhibited high reproducibility despite being a more simplified and rapid procedure compare to the GC-MS method. Phenyl 4-hydroxybenzoate was used as an internal standard because 1,3,5-tribromobenzene which had been used in the GC-MS method was not suitable compound for LC-MS/MS with Electrospray ionization (ESI) negative detection. The linear regression of the peak area ratios versus concentrations was fitted over the concentration ranges of 0.5-250μM and 0.25-250μM in blood and urine, respectively. The validation results satisfied the acceptance criteria for intra- and inter-day accuracy and precision. Blood and urine samples from 12 suspected HSP cases were tested using this method. The thiosulfate concentration detected in the sample coincided well with that determined at the scene of each HSP accident.
  • Maki Mizukami, Misaki Yamada, Sayaka Fukui, Nao Fujimoto, Shigeru Yoshida, Sanae Kaga, Keiko Obata, Shigeki Jin, Keiko Miwa, Nobuo Masauzi
    Journal of Clinical Laboratory Analysis 30 6 918 - 923 2016年11月 [査読有り]
  • Shigeki Jin, Hideki Hyodoh, Kotaro Matoba, Fei Feng, Akira Hayakawa, Katsuhiro Okuda, Keiko Shimizu, Sanae Haga, Michitaka Ozaki, Koichi Terazawa
    LEGAL MEDICINE 22 18 - 22 2016年09月 [査読有り][通常論文]
     
    Thiosulfate measurement is crucial to diagnosis of hydrogen sulfide (H2S) poisoning in forensic toxicology. Although GC-MS method is currently regarded as a standard thiosulfate measurement, it requires complicated sample preparation prior to analysis. This study presents a simple, rapid, and highly sensitive method for the quantitative analysis of serum thiosulfate by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method is based on selected reaction monitoring and has high sensitivity with a lower quantification limit of 0.5 mu M. Precision and accuracy of this method meet the basic requirements for quantitative analysis (intra- and inter-day tests have a relative standard deviation of <= 10.4%;range of analytical recovery is 94.3-102.6%). On the measurements of serum thiosulfate by our developed method, a thiosulfate concentration as 57.5 mu M was detected clearly in the H2S poisoning case comparing to the non poisoning case in which only a trace amount of thiosulfate was observed. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
  • Hayashi Nobuyuki, Jin Shigeki, Ujihara Tomomi
    TETRAHEDRON LETTERS 56 41 5557 - 5560 2015年10月07日 [査読有り][通常論文]
  • Fuda H, Watanabe M, Hui SP, Joko S, Okabe H, Jin S, Takeda S, Miki E, Watanabe T, Chiba H, These authors contributed equally to the work
    Food Chemistry 176 226 - 233 2015年06月 [査読有り][通常論文]
  • Shrestha R, Hui SP, Sakurai T, Yagi A, Takahashi Y, Takeda S, Jin S, Fuda H, Chiba H
    Annals of clinical biochemistry 51 Pt 6 662 - 671 2014年11月 [査読有り][通常論文]
     
    Impact Factor (2014): 2.335
    Cite Score (2014): 1.71
  • Ohkawa F, Takeda S, Hui SP, Sakurai T, Jin S, Fuda H, Sueoka K, Chiba H
    IEEE Sensors J. 14 2 532 - 537 2014年02月 [査読有り][通常論文]
  • Hayashi Nobuyuki, Ujihara Tomomi, Jin Shigeki
    TETRAHEDRON 70 4 845 - 851 2014年01月28日 [査読有り][通常論文]
  • Sakurai T, Takeda S, Takahashi JY, Takahashi Y, Wada N, Trirongjitmoah S, Namita T, Jin S, Ikuta A, Furumaki H, Hui SP, Fuda H, Fujikawa M, Shimizu K, Chiba H
    Annals of clinical biochemistry 50 Pt 6 564 - 570 2013年11月 [査読有り][通常論文]
     
    Impact Factor (2013): 2.080
    Cite Score (2013): 1.79
  • Takeda S, Ohkawa F, Hui SP, Sakurai T, Jin S, Fuda H, Sueoka K, Chiba H
    IEEE SENSORS J. 13 9 3449 - 3453 2013年09月 [査読有り][通常論文]
  • Sakurai T, Wada N, Takahashi Y, Ichikawa A, Ikuta A, Furumaki H, Hui SP, Jin S, Takeda S, Fuda H, Fujikawa M, Shimizu C, Nagasaka H, Furukawa H, Kobayashi S, Chiba H
    Annals of clinical biochemistry 50 Pt 5 465 - 472 2013年09月 [査読有り][通常論文]
     
    Impact Factor (2013): 2.080
    Cite Score (2013): 1.79
  • Jin S, Wada N, Takahashi Y, Hui SP, Sakurai T, Fuda H, Takeda S, Fujikawa M, Yanagisawa K, Ikegawa S, Kurosawa T, Chiba H
    Annals of clinical biochemistry 50 Pt 5 450 - 456 2013年09月 [査読有り][通常論文]
     
    Impact Factor (2013): 2.080
    Cite Score (2013): 1.79
  • Hui SP, Sakurai T, Takeda S, Jin S, Fuda H, Kurosawa T, Chiba H
    Anal Bioanal Chem 405 14 4981 - 4987 2013年05月 [査読有り][通常論文]
  • Takeda S, Hui SP, Fuda H, Jin S, Sakurai T, Ishii A, Mukasa K, Sueoka K, Chiba H
    J Biomed Nanotechnol 9 2 303 - 306 2013年02月 [査読有り][通常論文]
  • Seiji Takeda, Toshihiro Sakurai, Futaba Ohkawa, Shigeki Jin, Shu-Ping Hui, Hirotoshi Fuda, Koichi Mukasa, Hitoshi Chiba, Kazuhisa Sueoka
    2013 IEEE SENSORS 1038 - 1041 2013年 [査読有り][通常論文]
     
    Carbon nanotubes are sometimes treated with an acid or mixture of acids to increase their solubility. From results of our study of single-walled carbon nanotubes, we reported that a CNT-based electrode can detect oxidation of low density-lipoproteins. As described herein, we investigated the effects of acid treatment time on electrode sensitivity for detection of oxidized low-density lipoproteins. Response capabilities of CNT-based electrodes for detecting ox-LDL were improved by acid treatment. However, further increased treatment time degraded their sensitivity. Although detailed mechanisms related to the response are under investigation, results show that an optimum condition exists for acid treatment condition to detect ox-LDL using an electrode.
  • Hayashi N, Jin S, Ujihara T
    The Journal of organic chemistry 77 21 9652 - 9658 21 2012年11月 [査読有り][通常論文]
     
    To recognize alpha-amino acids with highly polar side chains in water, poorly water soluble scandium complexes with both Lewis acidic and basic portions were synthesized as artificial receptors. A suspension of some of these receptor molecules in an alpha-amino acid solution could remove acidic and basic alpha-amino acids from the solution. The compound most efficient at preferentially removing basic alpha-amino acids (arginine, histidine, and lysine) was the receptor with 7,7'-[1,3-phenylenebis(carbonylimino)]bis(2-naphthalenesulfonate) as the ligand. The neutral a-amino acids were barely removed by these receptors. Removal experiments using a mixed amino acid solution generally gave results similar to those obtained using solutions containing a single amino acid. The results demonstrated that the scandium complex receptors were useful for binding acidic and basic alpha-amino acids.
  • Mitsugu Watanabe, Hirotoshi Fuda, Shigeki Jin, Toshihiro Sakurai, Shu-Ping Hui, Seiji Takeda, Takayuki Watanabe, Takao Koike, Hitoshi Chiba
    FOOD CHEMISTRY 134 4 2086 - 2089 2012年10月 [査読有り][通常論文]
     
    3,5-Dihydroxy-4-methoxybenzyl alcohol (DHMBA), an antioxidant isolated from the Pacific oyster (Crassostrea gigas), was studied in a cell-based fluorometric antioxidant assay using human hepatocyte-derived cells (C3A) and diphenyl-1-pyrenylphosphine (DPPP) as a fluorescent probe. In comparison with two hydrophilic antioxidants. DHMBA showed the stronger inhibition of DPPP-mediated fluorescence than chlorogenic acid and L-ascorbic acid: at a concentration of 320 mu M of DPPP, the inhibition was 26.4 +/- 2.6%, 11.1 +/- 1.2%, and 0 +/- 2.0% for DHMBA, chlorogenic acid, and L-ascorbic acid, respectively (mean +/- SD, n = 4). Their relative oxygen radical absorbance capacities (ORAC) were dissociated with their cell-based antioxidant activities: 1.47 +/- 0.40, 4.57 +/- 0.30, and 0.53 +/- 0.13 mu mol TE/mu mol for DHMBA, chlorogenic acid, and L-ascorbic acid, respectively (mean +/- SD, n = 4). The amphiphilicity of DHMBA was better than chlorogenic acid and L-ascorbic acid might underlie this dissociation. Since the C3A cells are human hepatoma-derived cells, DHMBA might be useful in the prevention and treatment of liver diseases by involving an oxidation process. (C) 2012 Elsevier Ltd. All rights reserved.
  • 和田典男, 神繁樹, 藤谷昴文, 惠淑萍, 柳澤克之, 黒澤隆夫, 千葉仁志
    日本内分泌学会雑誌 88 2 695  2012年09月20日 [査読無し][通常論文]
  • Toshihiro Sakurai, Ayako Ichikawa, Hiroyuki Furukawa, Norio Wada, Atsushi Nagasaka, Yuji Takahashi, Masato Fujikawa, Akiko Ikuta, Hiroaki Furumaki, Maiko Shiga, Chikara Shimizu, Shu-Ping Hui, Shigeki Jin, Seiji Takeda, Hirotoshi Fuda, Hironori Nagasaka, Seiichi Kobayashi, Hitoshi Chiba
    ANNALS OF CLINICAL BIOCHEMISTRY 49 5 456 - 462 2012年09月 [査読有り][通常論文]
     
    Background: Triglyceride-rich low-density lipoproteins (TG-rich LDLs) in the plasma of patients with severe liver disease are reported to change macrophages into foam cells in vitro. Methods: Male BALB/c mice were immunized with TG-rich LDLs isolated from the plasma of a patient with severe liver disease. The resulting monoclonal antibody (G11-6) was used in a sandwich enzyme-linked immunosorbent assay (ELISA) in combination with polyclonal anti-apolipoprotein B antibodies. The time course of copper-mediated LDL oxidation was monitored using this ELISA. The results were compared with those of the two commercial ELISAs for oxidized LDLs using DLH or ML25, thiobarbituric acid reactive substances and the optical absorbance for the conjugated dienes generated in lipid peroxides. Furthermore, the lipoprotein fractions separated by gel filtration were tested with this ELISA in healthy volunteers (n = 11) and patients (n = 3) with liver disease. Results: G11-6 reacted with oxidized LDLs during only the early phase of copper oxidation, being distinct from the other monoclonal antibodies and methods. G11-6 was confirmed to react with TG-rich LDLs in patients, while it reacted with small LDL particles in normal controls. Conclusions: The monoclonal antibody G11-6 is useful for detecting oxidized small LDLs in normal controls and oxidized TG-rich LDLs in patients with severe liver disease.
  • Shu-Ping Hui, Toshihiro Sakurai, Futaba Ohkawa, Hiroaki Furumaki, Shigeki Jin, Hirotoshi Fuda, Seiji Takeda, Takao Kurosawa, Hitoshi Chiba
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY 404 1 101 - 112 2012年07月 [査読有り][通常論文]
     
    Oxidation of cholesteryl esters in lipoproteins by reactive oxygen species yields cholesteryl ester hydroperoxides (CEOOH). In this study, we developed a novel method for identification and characterization of CEOOH molecules in human lipoproteins by use of reversed-phase liquid chromatography with an hybrid linear ion trap-Orbitrap mass spectrometer (LC-LTQ Orbitrap). Electrospray ionization tandem mass spectrometric analysis was performed in both positive-ion and negative-ion modes. Identification of CEOOH molecules was completed by use of high-mass-accuracy (MA) mass spectrometric data obtained by using the spectrometer in Fourier-transform (FT) mode. Native low-density lipoproteins (nLDL) and native high-density lipoproteins (nHDL) from a healthy donor were oxidized by CuSO4, furnishing oxidized LDL (oxLDL) and oxidized HDL (oxHDL). No CEOOH molecules were detected in the nLDL and the nHDL, whereas six CEOOH molecules were detected in the oxLDL and the oxHDL. In positive-ion mode, CEOOH was detected as [M + NH4](+) and [M + Na](+) ions. In negative-ion mode, CEOOH was detected as [M + CH3COO](-) ions. CEOOH were more easily ionized in positive-ion mode than in negative-ion mode. The LC-LTQ Orbitrap method was applied to human plasma and six species of CEOOH were detected. The limit of detection was 0.1 pmol (S/N = 5:1) for synthesized CEOOH.
  • Shu-Ping Hui, Yudai Taguchi, Seiji Takeda, Futaba Ohkawa, Toshihiro Sakurai, Shinobu Yamaki, Shigeki Jin, Hirotoshi Fuda, Takao Kurosawa, Hitoshi Chiba
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY 403 7 1831 - 1840 2012年06月 [査読有り][通常論文]
     
    1-Palmitoyl-2-linoleoylphosphatidylcholine monohydroperoxide (PC 16:0/18:2-OOH) and 1-stearoyl-2-linoleoylphosphatidylcholine monohydroperoxide (PC 18:0/18:2-OOH) were measured by liquid chromatography/mass spectrometry (LC/MS) using nonendogenous 1-palmitoyl-2-heptadecenoylphosphatidylcholine monohydroperoxide as an internal standard. The calibration curves for synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, which were obtained by direct injection of the internal standard into the LC/MS system, were linear throughout the calibration range (0.8-12.8 pmol). Within-day and between-day coefficients of variation were less than 10%, and the recoveries were between 86% and 105%. The limit of detection (LOD) and the limit of quantification (LOQ) were determined using synthetic standards. The LOD (signal-to-noise ratio 3:1) was 0.01 pmol, and the LOQ (signal-to-noise ratio 6:1) was 0.08 pmol for both PC 16:0/18:2-OOH and PC 18:0/18:2-OOH. With use of this method, the concentrations of PC 16:0/18:2-OOH and PC 18:0/18:2-OOH in the lipoprotein fractions during copper-mediated oxidation were determined. We prepared oxLDL and oxHDL by incubating native LDL and native HDL from human plasma (n = 10) with CuSO4 for up to 4 h. The time course of the PC 16:0/18:2-OOH and PC 18:0/18:2-OOH levels during oxidation consisted of three phases. For oxidized LDL, both compounds exhibited a slow lag phase and a subsequent rapidly increasing propagation phase, followed by a gradually decreasing degradation phase. In contrast, for oxidized HDL, both compounds initially exhibited a prompt propagation phase with a subsequent plateau phase, followed by a rapid degradation phase. The analytical LC/MS method for phosphatidylcholine hydroperoxides might be useful for the analysis of biological samples.
  • Seiji Takeda, Shu-Ping Hui, Keisuke Fukuda, Hirotoshi Fuda, Shigeki Jin, Toshihiro Sakurai, Atsushi Ishii, Koichi Mukasa, Kazuhisa Sueoka, Hitoshi Chiba
    SENSORS AND ACTUATORS B-CHEMICAL 166 833 - 836 2012年05月 [査読有り][通常論文]
     
    Oxidized low-density lipoproteins (LDLs) play a key role in cardiovascular disease development, but no convenient measurement method is available for them. Using a carbon nanotube (CNT) electrode, we measured oxidized LDL using amperometric detection. Treating SWCNT with a mixture of acids produced a CNT dispersion that yielded nanotube-based electrodes after deposition on a gold electrode and drying. Current was monitored in the nanotube electrode before and after adding LDL or oxidization of LDL Oxidized LDL changed the current more than 10 nA, although LDL addition induced no significant change. Our CNT electrode enables simple detection of oxidized LDL. (C) 2012 Elsevier B.V. All rights reserved.
  • Mitsugu Watanabe, Hirotoshi Fuda, Shigeki Jin, Toshihiro Sakurai, Futaba Ohkawa, Shu-Ping Hui, Seiji Takeda, Takayuki Watanabe, Takao Koike, Hitoshi Chiba
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60 3 830 - 835 2012年01月 [査読有り][通常論文]
     
    Using an oxygen radical absorbance capacity (ORAC) assay, antioxidant activity was detected in the ethanol extract of the Pacific oyster, which was purified by sequential extraction with organic solvents. The ethyl acetate fraction showed the strongest antioxidant activity and was further purified, yielding a single compound [as assessed by thin-layer chromatography (TLC) reverse-phase high-performance liquid chromatography (HPLC)]. This compound was identified as 3,5-dihydroxy4-metboxybenzyl alcohol on the basis of H-1 and C-13 nuclear magnetic resonance (NMR), heteronuclear multiple-bond correlz.tion (HMBC), and electrospray ionization-mass spectrometry (ESI-MS) spectral analyses, a conclusion that was confirmed bycheinical synthesis. The concentration of the compound was 6.7 mg/100 g of whole oyster meat wet weight. This azaphi'philic gritiosidant retarded the copper mediated oxidation of low density lipoproteins (LDLs) and the generation of,tr.ickarbitonc acid.teactive substances. Furthermore, the compound showed substantial antioxidant activity using the ORAC and 2,2-diphenyl-1-picrythy'drazyl (DPPH) assays compared to natural antioxidants. Although the same compound was previously found in brown algae, its presence in other organisms and antioxidant activity are reported here for the first time.
  • Seiji Takeda, Futaba Ohkawa, Toshihiro Sakurai, Shigeki Jin, Hirotoshi Fuda, Shu-Ping Hui, Hitoshi Chiba, Kazuhisa Sueoka
    2012 IEEE SENSORS PROCEEDINGS 1751 - 1754 2012年 [査読有り][通常論文]
     
    Investigation of the properties of oxidized low-density lipoprotein is important because it is a risk factor of cardiovascular disease development. Low-density lipoprotein is known to have a positive charge on the surface attributable to amino groups of lysine moieties. Its positive charge can be decreased by oxidation. For this study, the topography and surface potential of LDL and ox-LDL were measured using atomic force microscopy and Kelvin probe force microscopy. The Kelvin probe force microscopy images of ox-LDL differ from those obtained using n-LDL. We demonstrated a method to evaluate the oxidation level of LDL using AFM and KPFM.
  • Takeshi Senoura, Shigeaki Ito, Hidenori Taguchi, Mariko Higa, Shigeki Hamada, Hirokazu Matsui, Tadahiro Ozawa, Shigeki Jin, Jun Watanabe, Jun Wasaki, Susumu Ito
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 408 4 701 - 706 2011年05月 [査読有り][通常論文]
     
    The consecutive genes BF0771-BF0774 in the genome of Bacteroides fragilis NCTC 9343 were found to constitute an operon. The functional analysis of BF0772 showed that the gene encoded a novel enzyme, mannosylglucose phosphorylase that catalyzes the reaction, 4-O-beta-D-mannopyranosyl-D-glucose + Pi -> mannose-1-phosphate + glucose. Here we propose a new mannan catabolic pathway in the anaerobe, which involves 1,4-beta-mannanase (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772), finally progressing to glycolysis. This pathway is distributed in microbes such as Bacteroides, Parabacteroides, Flavobacterium, and (C) 2011 Elsevier Inc. All rights reserved.
  • Rodjana Opassiri, Janjira Maneesan, Takashi Akiyama, Busarakum Pomthong, Shigeki Jin, Atsuo Kimura, James R. Ketudat Cairns
    PLANT SCIENCE 179 3 273 - 280 2010年09月 [査読有り][通常論文]
     
    Rice Os4BGlu12 beta-glucosidase is a family 1 glycoside hydrolase, the transcript levels of which have previously been found to be induced in response to herbivore attack and salinity stress. Here, high levels of Os4bglu12 transcripts were also detected in the shoot during germination, in the leaf sheath and stem of mature rice plants under normal growth conditions. The transcripts of this gene were up-regulated in response to wounding, methyl jasmonate and ethephon in 10-day-old rice seedlings. Os4BGlu12 expressed in recombinant Escherichia coli hydrolyzed beta-(1,3;1,4)-glucooligosaccharides generated by the wounding-induced rice endo-(1,3;1,4)-beta-glucanase OsEGL1, suggesting that both enzymes may act in concert in remodeling of damaged cell wall. Among oligosaccharides tested, Os4BGlu12 hydrolyzed beta-(1,4)-linked glucooligosaccharides with highest catalytic efficiency (k(cat)/K(m) = 2.7-4.9 s(-1) mM(-1)) when the degree of polymerization ranged from 3 to 6. It also hydrolyzed the beta-(1,3)-linked disaccharide laminaribiose with high catalytic efficiency (k(cat)/K(m) = 4.5 s(-1) mM(-1)). Among the natural glycosides tested, Os4BGlu12 efficiently hydrolyzed deoxycorticosterone 21-glucoside (k(cat)/K(m) = 20 s(-1) mM(-1)) and apigenin 7-O-beta-D-glucoside(k(cat)/K(m) = 6.7 s(-1) mM(-1)). The amino acid residues predicted to line the active site of Os4BGlu12 are more similar to those of cyanogenic and flavonoid beta-glucosidases than oligosaccharide hydrolases, and it may function in defense, as well as in cell wall-derived oligosaccharide break-down. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
  • Toshihiro Sakurai, Suchin Trirongjitmoah, Yuka Nishibata, Takeshi Namita, Masahiro Tsuji, Shu-Ping Hui, Shigeki Jin, Koichi Shimizu, Hitoshi Chiba
    ANNALS OF CLINICAL BIOCHEMISTRY 47 5 476 - 481 2010年09月 [査読有り][通常論文]
     
    Background: A simple method for the measurement of LDL particle sizes is needed in clinical laboratories because a predominance of small, dense LDL (sd LDL) has been associated with coronary heart disease. We applied dynamic light scattering (DLS) to measure lipoprotein particle sizes, with special reference to sd LDL. Methods: Human serum lipoproteins isolated by a combination of ultracentrifugation and gel chromatography, or by sequential ultracentrifugation, were measured for particle size using DLS. Results: The sizes of polystyrene beads, with diameters of 21 and 28 nm according to the manufacturer, were determined by DLS as 19.3 +/- 1.0 nm (mean +/- SD, n = 11) and 25.5 +/- 1.0 nm, respectively. The coefficients of variation for the 21 and 28 nm beads were 5.1% and 3.8% (within-run, n = 11), and 2.9% and 6.2% (between-run, n = 3), respectively. The lipoprotein sizes determined by DLS for lipoprotein fractions isolated by chromatography were consistent with the elution profile. Whole serum, four isolated lipoprotein fractions (CM + VLDL + IDL, large LDL, sd LDL and HDL) and a non-lipoprotein fraction isolated by sequential ultracentrifugation were determined by DLS to be 13.1 +/- 7.5, 37.0 +/- 5.2, 21.5 +/- 0.8, 20.3 +/- 1.1, 8.6 +/- 1.5 and 8.8 +/- 2.0 nm, respectively. Conclusions: The proposed DLS method can differentiate the sizes of isolated lipoprotein particles, including large LDL and sd LDL, and might be used in clinical laboratories in combination with convenient lipoprotein separation.
  • Shu-Ping Hui, Hitoshi Chiba, Shigeki Jin, Hironori Nagasaka, Takao Kurosawa
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878 20 1677 - 1682 2010年06月 [査読有り][通常論文]
     
    A new liquid chromatography mass spectrometry (LC/MS) method has been developed for the qualitative and quantitative analyses of phosphatidylcholine hydroperoxides (PC-OOH) in human plasma using a synthetic hydroperoxide (1-stearoyl-2-erucoyl-PC monohydroperoxide, PC 18 0/22 1-OOH) as an internal standard 1-Stearoyl-2-linoleoyl-PC monohydroperoxide (PC 18 0/18 2-OOH) was identified in plasma by LC/MS by comparison with an authentic standard The calibration curves obtained for 1-palmitoyl-2-linoleoyl-PC monohydroperoxide. PC 16 0/18 2-OOH and PC 18.0/18 2-OOH were linear throughout the calibration range (0 1-1 0 pmol) The limit of detection (LOD) (S/N = 3 1) was 0 01 pmol, and the limit of quantification (LOQ) (S/N = 6 1) was 0 1 pmol for both PC 16 0/18 2-OOH and PC 18.0/18.2-OOH Plasma concentrations of PC 16 0/18 2-OOH and PC 18 0/18 2-OOH were 89 and 32 nM, respectively, in a healthy volunteer (C) 2010 Elsevier B V All rights reserved
  • Takashi Akiyama, Shigeki Jin, Midori Yoshida, Tamotsu Hoshino, Rodjana Opassiri, James R. Ketudat Cairns
    JOURNAL OF PLANT PHYSIOLOGY 166 16 1814 - 1825 2009年 [査読有り][通常論文]
     
    We isolated two rice endo-(1,3;1,4)-beta-glucanase genes, denoted OsEGL1 and OsEGL2, which encoded proteins that shared 64% amino acid sequence identity. Both the OsEGL1 and OsEGL2 genes were successfully expressed in Escherichia coli to produce functional proteins. Purified OsEGL1 and OsEGL2 proteins hydrolyzed (1,3;1,4)-beta-glucans, but not (1,3;1,6)-beta-linked or (1,3)-beta-linked glucopolysaccharides nor carboxymethyl. cellulose, similar to previously characterized grass endo-(1,3;1,4)-beta-glucanases. RNA blot analysis revealed that the OsEGL1 gene is expressed constitutively not only in young roots of rice seedlings, but also in mature roots of adult rice plants. Little or no expression of the OsEGL2 gene was observed in all tissues or treatments tested, but database and RT-PCR analysis indicated it is expressed in ripening panicle. In rice seedling leaves, OsEGL1 gene expression significantly increased in response to methyl jasmonate, abscisic acid, ethephon and mechanical wounding. Mechanical wounding also increased the leaf elongation rate in rice seedlings by 16% relative to that of control seedlings at day 4 after treatment. The increase in the leaf elongation rate of rice seedlings treated under mechanical wounding was concomitant with an increase in OsEGL1 expression levels in seedling leaves. (C) 2009 Elsevier GmbH. All rights reserved.
  • Motoki Matsuda, Yuichiro Otsuka, Shigeki Jin, Jun Wasaki, Jun Watanabe, Toshihiro Watanabe, Mitsuru Osaki
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 366 2 414 - 419 2008年02月 [査読有り][通常論文]
     
    Peat contains various persistent compounds derived from plant materials. We isolated a novel (+)-catechin-degrading bacterium, Burkholderia sp. KTC-1 (KTC-1), as an example of a bacterium capable of degrading persistent aromatic compounds present in tropical peat. This bacterium was isolated by an enrichment technique and grew on (+)-catechin as the sole carbon source under acidic conditions. The reaction of a crude enzyme extract and a structural study of its products showed that (+)-catechin is biotransformed into taxifolin during the preliminary stages of its metabolism by KTC-1. HPLC analysis showed that this transformation occurs in two oxidation steps: 4-hydroxylation and dehydrogenation. Furthermore, both (+)-catechin 4-hydroxylanase and leucocyanidin 4-dehydrogenase were localized in the cytosol of KTC-1. This is the first report on biotransformation of (+)-catechin into taxifolin via leucocyanidin by an aerobic bacterium. We suggest that tropical peat could become a unique resource for microorganisms that degrade natural aromatic compounds. (C) 2007 Elsevier Inc. All rights reserved.
  • Osamu Kawase, Yoshifumi Nishikawa, Hiroshi Bannai, Houshuang Zhang, Guohong Zhang, Shigeki Jin, Eung-Goo Lee, Xuenan Xuan
    PROTEOMICS 7 20 3718 - 3725 2007年10月 [査読有り][通常論文]
     
    Toxoplasma gondii is an intracellular protozoan parasite that invades a wide range of nucleated cells. In the course of intracellular parasitism, the parasite releases a large variety of proteins from three secretory organelles, namely, micronemes, rhoptries and dense granules. Elevation of intracellular Ca (2+) in the parasite causes microneme discharge, and microneme, secretion is essential for the invasion. In this study, we performed a proteomic analysis of the Ca2+-dependent secretion to evaluate the protein repertoire. We found that Ca2+-mobilising agents, such as thapsigargin, NH4Cl, ethanol and a Ca2+ ionophore, A23187, promoted the secretion of the parasite proteins. The proteins, artificially secreted by A23187, were used in a comparative proteomic analysis by 2-DE followed by PMF analysis and/or N-terminal sequencing. Major known microneme proteins (MICs), such as MIC2, MIC4, MIC6 and MIC10 and apical membrane antigen 1 (AMA1), were identified, indicating that the proteomic analysis worked accurately. Interestingly, new members of secretory proteins, namely rhoptry protein 9 (ROP9) and Toxoplasma SPATR (TgSPATR), which was a homologue of a Plasmodium secreted protein with an altered thrombospondin repeat (SPATR), were detected in Ca2+-dependent secretion. Thus, we succeeded in detecting Ca2+-dependent secretory proteins in T.gondii,which contained novel. secretory proteins.
  • Takashi Akiyama, Shigeki Jin
    JOURNAL OF PLANT PHYSIOLOGY 164 5 645 - 654 2007年05月 [査読有り][通常論文]
     
    We cloned a rice cDNA encoding a putative arginine decarboxylase (ADC) protein, a key enzyme involved with putrescine (Put) biosynthesis in plants. The isolated full-length cDNA (OsADC1) contains an insert consisting of 2451 bp. The Longest open reading frame within encodes a putative protein of 702 amino acids, with a calculated molecular mass of 74kDa and an isoelectric point of 4.9. ClustalW alignment revealed that the deduced OsADC1 protein sequence shares overall 60% and 61% identity at the amino acid level with the Pisum sativum and Glycine max ADC proteins, respectively. Additionally, several OsADC1 regions exhibited striking similarity with these two other plant ADC protein sequences, including motifs characteristic of ADC proteins. Further, RNA get blot analysis revealed markedly increased OsADC1 mRNA levels in rice seedling leaves subjected to chilling stress. Interestingly, this treatment induced a concomitant increase in free Put levels in these samples, coincident with the observed elevated OsADC1 mRNA levels. To our knowledge, this represents the first direct evidence supporting essentially chilling-specific regulation of a rice ADC gene that also potentially influences Put accumulation, a phenomenon previously noted in cold-stressed rice seedlings. (C) 2006 Elsevier GmbH. All rights reserved.
  • Sun-Ju Kim, Chiami Kawaharada, Shigeki Jin, Makoto Hashimoto, Gensho Ishii, Hiroaki Yamauchi
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 71 1 114 - 121 2007年01月 [査読有り][通常論文]
     
    The structurally unique glucosinolate (GSL), 4-(cystein-S-yl)butyl GSL, was identified in the leaves of hydroponically-grown rocket salad (Eruca sativa Mill.). Its electrospray ionization mass spectrometry (ESI-MS)/MS spectrum indicated that this unusual GSL had a molecular weight of 414 as a desulfo (DS)-GSL, and a molecular formula of C14H25N2O8S2 based on its negative ion matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) spectrum. For further confirmation, the 4-(cystein-S-yl)butyl DS-GSL was prepared with authentic L-Ser and purified dimeric 4-mercaptobutyl DS-GSL, and its chemical structure then confirmed by ESI-MS/MS data. It is named "glucorucolamine" as a trivial name from its ammonia sensitivity. This unique GSL was found to the greatest extent when rocket salad was grown in a 100% NH4+-N nutrient solution. Despite it clearly seems to reduce the detoxification of excess NH4+ in the leaves of rocket salad, present knowledge about the unique GSL is still far from being sufficient.
  • Shigeki Jin, Jiro Nagao, Satoshi Takeya, Yusuke Jin, Junko Hayashi, Yasushi Kamata, Takao Ebinuma, Hideo Narita
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS 45 24-28 L714 - L716 2006年07月 [査読有り][通常論文]
     
    The structure of natural gas hydrate sediments was observed by microfocus X-ray computed tomography (CT). A newly developed high-pressure vessel for the microfocus X-ray CT system was applied to observe the sediments at a temperature above 273 K and under high-pressure conditions. The obtained two-dimensional CT images clearly showed the spatial distribution of the free-gas pore, sand particles, water, and hydrates. These results demonstrated that microfocus X-ray CT can be effective for studying natural gas hydrate sediment samples.
  • Jin S, Yoshida M
    Cryobiology and Cryotechnology 51 57 - 62 2005年12月 [査読有り][通常論文]
  • M Sato, S Takeya, J Nagao, S Jin, Y Kamata, H Minagawa, T Ebinuma, H Narita
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS 44 1A 473 - 475 2005年01月 [査読有り][通常論文]
     
    A high-speed X-ray computerized tomography (CT) system was developed for measuring the texture of methane hydrate sediment. The system enabled time dependent nondestructive observations of the hydrate sediment in a volume of phi100 X 100 mm(3) within 40s. The spatial variation of mass density and the volume fraction of an artificial methane hydrate sediment that is either ice or hydrate, hereafter referred to as the hydrate saturation ratio within methane hydrate sediments, were measured using the X-ray CT system. The three-dimensional (3D) distribution change of density and hydrate saturation caused by the hydrate dissociation were also visualized on the basis of the measured gray scale values of the CT images taken before and after their dissociation. The technique allows us observations of the dissociation. process of methane hydrate sediment under experimental conditions which are the same as conditions in the natural environment, and will allow the simulation of methane gas production from wells in both oceanic and permafrost areas.
  • JH Yoon, T Kawamura, S Takeya, SK Jin, Y Yamamoto, T Komai, M Takahashi, AV Nawaby, YP Handa
    MACROMOLECULES 37 25 9302 - 9304 2004年12月 [査読有り][通常論文]
  • SJ Kim, S Jin, G Ishii
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 68 12 2444 - 2450 2004年12月 [査読有り][通常論文]
     
    A structurally unique glucosinolate (GSL) was identified to be 4-(beta-(D)-glucopyranosyldisulfanyl)butyl GSL in rocket leaves. The positive-ion electrospray ionization mass spectrometry (ESI-MS) data indicated that the new GSL had a molecular weight of 521 (m/z 522, [M + H](+), as desulfo-GSL). The molecular formula of the substance was determined to be C17H32O11NS3 (m/z 522.1143, [M + H](+)) based on its positive-ion high-resolution fast atom bombardment mass spectrometry (HR-FAB-MS) data. For the further confirmation, desulfated GSL of 4-(beta-(D)-glucopyranosyldisulfanyl)butyl GSL was prepared by commercial 1-thio-beta-(D)-glucose and dimeric 4-mercaptobutyl desulfo-GSL, which was also isolated from rocket leaves, and its chemical structure was then confirmed by MS data and nuclear magnetic resonance (NMR) spectroscopy. In addition, the antioxidative activity of 4-(beta-(D)-glucopyranosyldisulfanyl)butyl desulfo-GSL was measured by means of chemiluminescence (CL) for evaluating the functional properties. The antioxidative activity (2.089 unit/g) was relatively higher than that of dimeric 4-mercaptobutyl desulfo-GSL (1.227).
  • S Jin, S Takeya, J Hayashi, J Nagao, Y Kamata, T Ebinuma, H Narita
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS 43 8A 5673 - 5675 2004年08月 [査読有り][通常論文]
     
    The structure of natural gas hydrate sediments was characterized by microfocus X-ray computed tomography (CT). The obtained two-dimensional (2-D) and three-dimensional (3-D) images clearly showed the spatial distribution of the free-gas spaces, sand particles, and hydrates or ices. The estimated porosity from the X-ray CT data was consistent with the value that was obtained from the sample mass and volume. These results indicate that microfocus X-ray CT can be very useful for researching natural samples of hydrate sediments.
  • S Jin, M Yoshida, T Nakajima, A Murai
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 67 6 1245 - 1249 2003年06月 [査読有り][通常論文]
     
    It was found that the content of antifungal compounds p-coumaroylagmatine [1-(trans-4'-hydroxycinnamoylamino)-4-guanidinobutane] and p-coumaroyl-3-hydroxyagmatine [1-(trans-4'-hydroxycinnamoylamino)-3-hydroxy-4-guanidinobutane] in the crown of winter wheat (Triticum aestivum L. cv Chihokukomugi) significantly increased under snow cover. This finding suggests that the accumulation of these hydroxycinnamic acid amides was caused by winter stress and related to protecting the plant against snow mold under snow cover.
  • S Jin, N Sato
    PHYTOCHEMISTRY 62 1 101 - 107 2003年01月 [査読有り][通常論文]
     
    Aqueous extracts of the tissue of succulent young shoots of the pear Pyrus spp. exhibited strong antibacterial activity against the bacterium Erwinia amylovora bv. 4. This activity was investigated quantitatively by a newly developed bioassay method. It was found that the activity changed with the age of the tissue. Extracts of the youngest leaves and stems from the shoot tops showed the strongest activity, and the activity decreased with age of the leaves and stems. The activity also changed with increase in time after preparation of the extract, increasing rapidly in the first hour after preparation, reaching a maximum at about 4 h, and then decreasing slowly. The substance essential for the antibacterial activity was isolated from the extract by steam distillation in vacuo and through charcoal powder column chromatography. It was identified as benzoquinone (2,5-cyclohexadiene-1,4-dione) by NMR-spectra, mass spectra and HPLC analysis. The phenolic metabolism from arbutin to hydroquinone and then to benzoquinone in the aqueous extracts was analyzed quantitatively by HPLC. The changes in the contents of benzoquinone in the extracts of leaves and stems with tissue aging and with increase in time after preparation of the extracts paralleled the changes in antibacterial activity as determined by the quantitative bioassay. (C) 2003 Elsevier Science Ltd. All rights reserved.
  • S Jin, M Yoshida
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 64 8 1614 - 1617 2000年08月 [査読有り][通常論文]
     
    An antifungal compound, feruloylagmatine [1-(trans -4'-hydroxy-3'-methoxycinnamoylamino)-4-guanidinobutane], was isolated from crowns of winter wheat (Triticum aestivum L. cv Chihokukomugi). Its structure was identified by NMR, MS and UV spectral analyses. It was also confirmed by an HPLC analysis that the compound was induced in wheat by a low temperature.
  • H TAKASHIMA, S JIN, M YOSHIDA, J ABE, A MURAI
    CHEMISTRY LETTERS 11 1015 - 1016 1995年11月 [査読有り][通常論文]
     
    A metabolite has been isolated from winter wheat leaves inoculated with H2O2 and its structure was elucidated from the spectral data.

MISC

共同研究・競争的資金等の研究課題

  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2022年04月 -2025年03月 
    代表者 : 的場 光太郎, 神 繁樹
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2019年04月 -2022年03月 
    代表者 : 兵頭 秀樹, 的場 光太郎, 神 繁樹
     
    死後画像所見について、定量評価並びに死因に特徴的な画像所見を明らかにすること、特に薬毒物中毒等に対する死後画像による定量評価は期待されている。本研究では動物モデルで得られた評価基準をもとにヒトにおける死後画像による薬毒物中毒の診断指標を探索した。 最終年度までの検討により、薬毒物により死亡に至った可能性を疑わせる指標を得ることは可能であるが、高精度診断には限界があり、他の検査所見と併用することで診断可能と結論づけることができ、死後画像の役割を明確化できた。死後CT画像のみでも肝臓の脂肪変性を客観的に評価可能であり、代謝異常についても高精度で診断可能であることがあきらかとなった。
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2018年04月 -2022年03月 
    代表者 : 神 繁樹, 的場 光太郎
     
    様々な死後経過時間の死体の体液を化学分析し，腐敗度の高い死体から得られた検体から高濃度の酪酸が検出されることを見出した．非腐敗の検体と比較して平均値で血液において100倍以上，尿においては300倍以上の濃度の酪酸が検出された．これらの結果から体液中の酪酸濃度は腐敗の指標として有用であることが期待される．また，硫化水素中毒死ではない高腐敗度の検体から硫化水素中毒のマーカーとされるチオ硫酸塩が高濃度で検出され，中毒の鑑定に注意が必要であることが示された．
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2017年04月 -2019年03月 
    代表者 : 的場 光太郎, 兵頭 秀樹, 神 繁樹
     
    血液中のチオ硫酸塩濃度は硫化水素中毒の指標として注目されている。本研究では従来のGC-MSによる測定法に代わるLC-MS/MSによるチオ硫酸塩濃度測定法を確立し、３種の検体（血液・尿・心嚢液）について死後腐敗によるチオ硫酸塩濃度の変化を調査した。硫化水素中毒ではない場合でも３種の検体とも腐敗事例では高濃度のチオ硫酸塩が検出されることが多く、腐敗していない場合の硫化水素中毒事例と同等のレベルで検出されることが多かった。この結果から硫化水素中毒診断の指標としてチオ硫酸塩を用いる場合には腐敗の影響を十分に考慮する必要があることが示された。
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2013年04月 -2017年03月 
    代表者 : 神 繁樹, 千葉 仁志, 惠 淑萍, 和田 典男
     
    原発性アルドステロン症は高血圧症の原因の約10％とされ、治療法の異なるサブタイプの鑑別は重要である。本研究で我々はアルドステロン産生腺腫（APA）において他のサブタイプと比較して尿中に多く検出される物質を新たに見出し、新規バイオマーカーとしての可能性について検討した。物質の単離・構造決定までには至らなかったものの、高血圧患者検体の分析の結果、本態性高血圧や両側過形成を原因とする特発性アルドステロン症（IHA）に対して1.5～2倍の量が検出された。
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2013年04月 -2016年03月 
    代表者 : 布田 博敏, 平松 尚志, 神 繁樹, 神 繁樹
     
    マガキ由来の新規の抗酸化物質(DHMBA)において、肝培養細胞を用いた抗酸化能の観察、及び非アルコール性脂肪肝炎(NASH)モデルマウスを用いた肝病変の改善効果を検証した。肝培養細胞を用いた実験では、DHMBAは酸化剤に対する細胞保護作用や酸化誘導性のアポトーシスに対する抑制効果が観察された。NASHモデルマウスを用いた実験では、DHMBA を高濃度含むマガキ抽出物によりNASHの肝臓特有に見られる病理組織学的症状の改善、及び抗肥満、インスリン抵抗性の改善が見られた。これらのことから、DHMBAは酸化ストレスが発生原因と考えられているNASHの予防や治療に有効と考えられた。
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2012年04月 -2015年03月 
    代表者 : 星 淡子, 神 繁樹
     
    日本人の約半数が遺伝子変異を有するアルデヒド脱水素酵素(ALDH2)遺伝子の変異は骨粗鬆症を惹起する。この原因に基づく骨粗鬆症を予防するための機能性化合物の検討を行った。遺伝子変異による酵素代謝不全の結果、原因物質として体内に過剰の過酸化脂質とアルデヒドの蓄積を確認した。機能性物質として抗酸化物質を添加したアセトアルデヒド存在下の骨芽細胞の分化誘導能を検討した結果、アセトアルデヒドによる骨芽細胞の形成不全を効果的に回復させた。そこで、実際にALDH2遺伝子変異マウスへ抗酸化化合物を経口投与して骨密度の検討を行った結果、大腿骨密度は有意な上昇を示し、骨粗鬆症の症状を回復させることを確認した。

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