研究者データベース

川口 俊一(カワグチ トシカズ)
地球環境科学研究院 物質機能科学部門 分子材料化学分野
准教授

基本情報

所属

  • 地球環境科学研究院 物質機能科学部門 分子材料化学分野

職名

  • 准教授

学位

  • 博士 (地球環境科学)(北海道大学)

ホームページURL

J-Global ID

研究キーワード

  • システム工学   化学センサ   ナノテクノロジー   自己組織化膜   エネルギー   スーパーキャパシタ   界面制御技術   

研究分野

  • 環境・農学 / 環境材料、リサイクル技術
  • 環境・農学 / 環境負荷低減技術、保全修復技術

職歴

  • 2009年10月 - 現在 北海道大学 地球環境科学研究科(研究院) 准教授

研究活動情報

論文

  • Guizani Mokhtar, Yajima Kento, Kawaguchi Toshikazu, Ito Ryusei, Funamizu Naoyuki
    RECENT ADVANCES IN ENVIRONMENTAL SCIENCE FROM THE EURO-MEDITERRANEAN AND SURROUNDING REGIONS, VOLS I AND II 933 - 935 2018年 [査読有り][通常論文]
  • Li Long, Wanjun Liu, Hengheng Xia, Yao Ken, Kawaguchi Toshikazu, Shimazu Katsuaki
    SENSORS AND ACTUATORS B-CHEMICAL 213 248 - 251 2015年07月 [査読有り][通常論文]
     
    A novel two-dimensional organic-inorganic hybrid material containing mouse immunoglobulin G (layered carboxylpropylamidephenylsilica-mouse immunoglobulin G: CPAPhS-mouse IgG) was developed to enhance the sensitivity of the chip used for surface plasmon resonance (SPR). Mouse IgG was covalently attached to the layered CPAPhS using well-established active ester chemistry. CPAPhS-mouse IgG was facilely fabricated onto the Au surface to acquire a novel surface plasmon resonance (SPR) sensor chip, which exhibits higher sensitivity in the detection of the anti-mouse IgG. More interestingly, two different kinds of adsorption equilibrium were exhibited in the unique sensorgram of the anti-mouse IgG, likely due to the unique material structure of the sensor chip. (C) 2015 Elsevier B.V. All rights reserved.
  • Vladimir V. Plashnitsa, Taro Ueda, Perumal Elumalai, Toshikazu Kawaguchi, Norio Miura
    Ionics 14 1 15 - 25 2008年02月 [査読有り][通常論文]
     
    The nanostructured thin NiO films with the thicknesses of 30-180 nm were examined as a sensing electrode (SE) for the planar mixed-potential-type yttria-stabilized zirconia (YSZ)-based NO2 sensor. The sensing characteristics were examined in the temperature range of 600-800 °C under the wet condition (5 vol.% water vapor). Among the NiO-SEs tested, the 60 nm-thick NiO-SE sintered at 1,000 °C was found to give the highest NO 2 sensitivity in the NO2 concentration range of 50-400 ppm accompanying with fast response/recovery at the operating temperatures of 600-700 °C. The high NO2 sensitivity was attributed to the high catalytic activity for both electrochemical reactions of O2 and NO2 at the interface of NiO-SE/YSZ. The ultrathin gold layer with the thickness of about 60 nm was additionally formed on the 60 nm-thick NiO-SE to fabricate the laminated-type (60 nm NiO/60 nm Au)-SE. It was demonstrated that the use of this laminated (NiO-Au)-SE improved both the sensitivity and the selectivity to NO2. ? 2007 Springer-Verlag.
  • Toshikazu Kawaguchi, Dhesingh Ravi Shankaran, S. J. Kim, K. Vengatajalabathy Gobi, Kiyoshi Matsumoto, Kiyoshi Toko, Norio Miura
    TALANTA 72 2 554 - 560 2007年04月 [査読有り][通常論文]
     
    We have developed a new immunosensor based on self-assembly chemistry for highly sensitive and label-free detection of 2,4,6-trinitrotoluene (TNT) using surface plasmon resonance (SPR). A monolayer of amine terminated poly(ethylene glycol) hydrazinehydrochloride (PEG-NH2) thiolate was constructed on an activated gold surface and immobilized with trinitrophenyl-beta-alanine (TNPh-beta-alanine) by amide coupling method. The binding interaction of a monoclonal anti-TNT Ab (M-TNT Ab) with TNPh-beta-alanine immobilized thiolate monolayer surface was monitored and evaluated for detection of TNT based on the principle of indirect competitive immunoreaction. Here, the competition between the self-assembled TNT derivative and the TNT in solution for binding with antibody yields in the response signal that is inversely proportional to the concentration of TNT in the linear detection range. With the present immunoassay format, TNT could be detected in the concentration range from 0.008 ng/ml (8 ppt) to 30 ng/ml (30 ppb). The response time for an immunoreaction was 2 min and one immunocycle could be done with in 4 min including surface regeneration. Bound antibodies could be easily eluted from the self-assembled immunosurface at high recoveries (more than 100 cycles) using pepsin solution without any damage to the TNT derivatives immobilized on the surface. The compact self-assembled monolayer was highly stable and prevented the non-specific adsorption of proteins on the surface favoring error free measurement. (c) 2006 Elsevier B.V. All rights reserved.

その他活動・業績

  • 川口俊一, KABIRAZ Dulal, 森田金市, 高橋昌志 電気化学会大会講演要旨集(CD-ROM) 85th ROMBUNNO.1B12 2018年02月23日 [査読無し][通常論文]
  • KABIRAZ Dulal, 森田 金市, 川口 俊一, 高橋 昌志 Proceedings of the Chemical Sensor Symposium 62 67 -69 2017年09月 [査読無し][通常論文]
  • KABIRAZ Dulal, 森田金市, 川口俊一, 川口俊一 日本分析化学会年会講演要旨集 65th 115 2016年08月31日 [査読無し][通常論文]
  • 鎌田詢也, 上面雅義, 川口俊一, 田中俊逸 日本分析化学会年会講演要旨集 65th 323 2016年08月31日 [査読無し][通常論文]
  • 瀧谷明義, 大屋光平, 羽深昭, 川口俊一, 高橋正宏, 佐藤久 日本水環境学会年会講演集 50th 114 2016年03月10日 [査読無し][通常論文]
  • 鎌田詢也, 上面雅義, 川口俊一, 田中俊逸 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM) 2016 ROMBUNNO.P50 2016年 [査読無し][通常論文]
  • 高橋慧良, 上面雅義, 川口俊一, 田中俊逸 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM) 2016 ROMBUNNO.P45 2016年 [査読無し][通常論文]
  • 大嶋美奈子, 堤友貴, 須永将光, 松野穣, 秋津貴城, 原賢二, 川口俊一 錯体化学会討論会講演要旨集 65th 224 2015年09月01日 [査読無し][通常論文]
  • 山田健太, 矢嶋健人, 川口俊一, 石井聡, 佐野大輔, 高橋正宏, 佐藤久 日本水環境学会年会講演集 49th 504 2015年03月16日 [査読無し][通常論文]
  • 川口俊一, 大森徹, 中村英博, 森田金市, 坂槙有紀恵, 山田健太, 津田収, 佐藤久, 嶋津克明 Chem Sens 30 (Supplement A) 31 -33 2014年03月29日 [査読無し][通常論文]
  • 川口俊一, 大森徹, 中村英博, 森田金市, 坂槙有紀恵, 山田健太, 津田収, 佐藤久, 嶋津克明 電気化学会大会講演要旨集 81st 275 2014年03月29日 [査読無し][通常論文]
  • 坂槙有紀恵, 山田健太, 津田収, 大森徹, 川口俊一, 高橋正宏, 岡部聡, 佐藤久 日本水環境学会年会講演集 48th 221 2014年03月17日 [査読無し][通常論文]
  • 川口 俊一 ぶんせき 0 (436) 229 -230 2011年04月05日 [査読無し][通常論文]
  • High-performance surface plasmon resonance immunosensors for TNT detection
    Norio Miura, Dhesingh Ravi Shankaran, Toshikazu Kawaguchi, Kiyoshi Matsumoto, Kiyoshi Toko ELECTROCHEMISTRY 75 (1) 13 -22 2007年01月 [査読無し][通常論文]
     
    Detection of 2,4,6-trinitrotoluene (TNT) is an important environmental, security and health concern for the global community. TNT is a prime constituent of most of the landmines and bombs and is highly toxic and mutagenic. Various military and terrorist activities (e.g., manufacturing, waste discharge, testing and training) have resulted in extensive contamination of soil and ground water by TNT and its derivatives. Consequently, the development and application of new sensing techniques for detection and quantification of TNT has grown steadily over the years. Despite wide variety of analytical techniques, surface plasmon resonance (SPR) based immunosensors received great attention as a promising mean for TNT detection due to their advantages including high sensitivity, selectivity, good versatility and high throughput. This review explores the recent trend and advancements in immunochemical techniques for environmental monitoring and field detection of TNT. The advantages of the surface plasmon resonance as an optical signal transduction and indirect competitive immunoassay as the sensing principle are discussed with special emphasis on our investigations on TNT detection. A brief description on explosives, landmines and the current detection techniques (bulk and trace detection) is also provided.
  • Dhesingh Ravi Shankaran, Toshikazu Kawaguchi, Sook Jin Kim, Kiyoshi Matsumoto, Kiyoshi Toko, Norio Miura INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY 87 (10-11) 771 -781 2007年 [査読無し][通常論文]
     
    Recent concern on international terrorism and weapons of mass destruction demands the development of novel analytical methods for identification and quantification of explosive molecules. In this article, we describe the development of high-performance immunosensors for detection of 2,4,6-trinitrotoluene (TNT), a prime component of the landmines and bombs used by terrorist and military forces. The immunosensors were constructed by physical adsorption and self-assembly methods, and their binding interactions with a monoclonal anti-TNT antibody were evaluated for TNT detection using the surface plasmon resonance technique. A home-made 2,4,6-trinitrophenyl-keyhole limpet hemocyanine conjugate was used for physical adsorption. A poly( ethylene glycol) hydrazine hydrochloride thiolate was used in the construction of self-assembled monolayer surface and was immobilized with trinitrophenyl- beta-alanine by the amide coupling method. The immunosensors were highly selective, regenerable, rapid, and exhibited remarkable sensitivity down to the parts-per-trillion level for TNT by the indirect competitive inhibition principle.
  • Dhesingh Ravi Shankaran, Toshikazu Kawaguchi, Sook Jin Kim, Kiyoshi Matsumoto, Kiyoshi Toko, Norio Miura ANALYTICAL AND BIOANALYTICAL CHEMISTRY 386 (5) 1313 -1320 2006年11月 [査読無し][通常論文]
     
    Detection of TNT is an important environmental and security concern all over the world. We herein report the performance and comparison of four immunoassays for rapid and label-free detection of 2,4,6-trinitrotoluene (TNT) based on surface plasmon resonance (SPR). The immunosensor surface was constructed by immobilization of a home-made 2,4,6-trinitrophenyl-keyhole limpet hemocyanin (TNPh-KLH) conjugate onto an SPR gold surface by simple physical adsorption within 10 min. The immunoreaction of the TNPh-KLH conjugate with four different antibodies, namely, monoclonal anti-TNT antibody (M-TNT Ab), monoclonal anti-trinitrophenol antibody (M-TNP Ab), polyclonal anti-trinitrophenyl antibody (P-TNPh Ab), and polyclonal anti-TNP antibody (P-TNP Ab), was studied by SPR. The principle of indirect competitive immunoreaction was employed for quantification of TNT. Among the four antibodies, the P-TNPh Ab prepared by our group showed highest sensitivity with a detection limit of 0.002 ng/mL (2 ppt) TNT. The lowest detection limits observed with other commercial antibodies were 0.008 ng/mL (8 ppt), 0.25 ng/mL (250 ppt), and 40 ng/mL (ppb) for M-TNT Ab, P-TNP Ab, and M-TNP Ab, respectively, in the similar assay format. The concentration of the conjugate and the antibodies were optimized for use in the immunoassay. The response time for an immunoreaction was 36 s and a single immunocycle could be done within 2 min, including the sensor surface regeneration using pepsin solution. In addition to the quantification of TNT, all immunoassays were evaluated for robustness and cross-reactivity towards several TNT analogs.

教育活動情報

主要な担当授業

  • 水土管理特論
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
  • 食資源環境論
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 畜産,農業,健康,生態系,汚染物質,気候変動,微生物,物質循環,環境管理
  • ワンダーフォーゲル実習Ⅲ
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
  • 環境解析とモニタリング特論演習
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 環境数理モデリング,水輸送現象,土壌物理分析,地理情報処理システム,環境情報学,機器分析,安定同位体地球化学,気象観測,リモートセンシング
  • 事前・事後演習Ⅲ
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
  • 国際プレゼンテーションスキル演習
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 実践英語,表現力育成,コミュニケーション能力育成,プレゼンテーション能力育成,議論力育成
  • 国際実践力演習
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 実践英語,論理,自己表現力
  • 文系のための自然科学基礎論
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 化学,生物,地学,物理
  • 環境管理特論演習
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 水環境,土壌環境,大気環境,環境マネージメント学,気象学,気候学,バイオマス,物質とエネルギー循環(炭素),物質とエネルギー循環,栄養循環
  • ワンダーフォーゲル実習Ⅲ
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 世界各地の食資源問題, ワイカト大学、アグリサーチ,アデレード大学,CSIRO,IRRI
  • 事前・事後演習Ⅲ
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 世界各地の食資源問題, ワイカト大学、アグリサーチ,アデレード大学,CSIRO,IRRI
  • 環境科学総論
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 環境科学院
    キーワード : Cold region, radioactive waste, underground final disposal, climate change, weather, ecosystem in Hokkaido, heavy metal toxicity, sustainable societies, research-community relationship, Future Earth
  • 大学院共通授業科目(一般科目):自然科学・応用科学
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 大学院共通科目
    キーワード : Cold region, radioactive waste, underground final disposal, climate change, weather, ecosystem in Hokkaido, heavy metal toxicity, sustainable societies, research-community relationship, Future Earth
  • 化学Ⅰ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 全学教育
    キーワード : 原子の構造、原子軌道、化学結合、混成軌道、物質の三態、電解質溶液
  • General Education Seminar
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 総合教育部
  • General Education Seminar
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 現代日本学プログラム課程
  • 国際交流Ⅱ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 国際本部


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