研究者データベース

陳 震(チン シン)
保健科学研究院 保健科学部門 健康科学分野
講師

基本情報

所属

  • 保健科学研究院 保健科学部門 健康科学分野

職名

  • 講師

学位

  • 医学博士(2016年06月 中国海洋大学)

研究キーワード

  • 食品化学   LC/MS   プラスマロゲン   ミトコンドリア   リピドミクス   過酸化脂質   

研究分野

  • ライフサイエンス / 栄養学、健康科学
  • ライフサイエンス / 代謝、内分泌学
  • ライフサイエンス / 食品科学

職歴

  • 2020年10月 - 現在 北海道大学 大学院保健科学研究院 講師
  • 2017年04月 - 2020年09月 北海道大学 大学院保健科学研究院 助教
  • 2016年07月 - 2017年03月 北海道大学 大学院保健科学研究院 博士研究員

所属学協会

  • 日本過酸化脂質・抗酸化物質学会   日本医用マススペクトル学会   日本臨床化学会   

研究活動情報

論文

  • Yasuyuki Tamai, Zhen Chen, Yue Wu, Jun Okabe, Yoshinao Kobayashi, Hitoshi Chiba, Shu-Ping Hui, Akiko Eguchi, Motoh Iwasa, Masaaki Ito, Yoshiyuki Takei
    Biomedicine & Pharmacotherapy 135 111181 - 111181 2021年03月
  • Yue Wu, Zhen Chen, Jiaping Jia, Hitoshi Chiba, Shu-Ping Hui
    Foods 10 1 124 - 124 2021年01月08日 
    Plasmalogens are an animal-derived functional phospholipid increasingly known as a safe and effective nutritional ingredient, however, the quantitation and comparison of plasmalogen species in foods is limited. In the present work, determination methods for dietary plasmalogens using liquid chromatography-tandem mass spectroscopy under positive and negative ionization modes were compared. The negative-mode method, which showed better selectivity, sensitivity, and accuracy, was then applied in 14 kinds of livestock, poultry, and seafood samples. Livestock and poultry showed abundant total plasmalogen (530.83–944.94 nmol/g), higher than fish (46.08–399.75 nmol/g) and mollusk (10.00–384.76 nmol/g). While fish and mollusk samples expressed healthier fatty acyl composition, with higher eicosapentaenoyl and more beneficial n-6/n-3 ratio than the land animal meats, especially for squid and octopus, with eicosapentaenoyl of 98.4% and 94.5%, respectively. The correlations among plasmalogen species varied in different foodstuffs with distinguishing patterns, suggesting the customizable strategies for achieving targeted plasmalogen species. These findings not only provided fundamental comparison of plasmalogen among daily foodstuffs, but also contributed to extend the dietary plasmalogen sources for health food development.
  • Chen, Zhen, Liang, Qiangrong, Wu, Yue, Gao, Zijun, Kobayashi, Satoru, Patel, Joy, Li, Cairong, Cai, Fei, Zhang, Youhua, Liang, Chongsheng, Chiba, Hitoshi, Hui, Shu-Ping
    Metabolomics 16 11 115 - 115 2020年11月 [査読有り][通常論文]
  • Yusuke Yamamoto, Toshihiro Sakurai, Zhen Chen, Takayuki Furukawa, Siddabasave Gowda B. Gowda, Yue Wu, Kazuhiro Nouso, Yuki Fujii, Yuki Yoshikawa, Hitoshi Chiba, Shu-Ping Hui
    Analytical and Bioanalytical Chemistry 2020年10月22日 [査読有り][通常論文]
  • Yusuke Yamamoto, Toshihiro Sakurai, Zhen Chen, Takayuki Furukawa, Siddabasave Gowda B. Gowda, Yue Wu, Kazuhiro Nouso, Yuki Fujii, Yuki Yoshikawa, Hitoshi Chiba, Shu-Ping Hui
    Analytical and Bioanalytical Chemistry 2020年10月22日 [査読有り][通常論文]
  • Yue Wu, Zhen Chen, Hitoshi Chiba, Shu-Ping Hui
    Food Chemistry 2020年04月 [査読有り][通常論文]
  • Koshi Nagai, Baasanjav Uranbileg, Zhen Chen, Amane Fujioka, Takahiro Yamazaki, Yotaro Matsumoto, Hiroki Tsukamoto, Hitoshi Ikeda, Yutaka Yatomi, Hitoshi Chiba, Shu-Ping Hui, Toru Nakazawa, Ritsumi Saito, Seizo Koshiba, Junken Aoki, Daisuke Saigusa, Yoshihisa Tomioka
    Rapid communications in mass spectrometry : RCM 34 Suppl 1 e8551  2020年04月 [査読有り][通常論文]
     
    RATIONALE: Hepatocellular carcinoma (HCC) is a highly malignant disease for which the development of prospective or prognostic biomarkers is urgently required. Although metabolomics is widely used for biomarker discovery, there are some bottlenecks regarding the comprehensiveness of detected features, reproducibility of methods, and identification of metabolites. In addition, information on localization of metabolites in tumor tissue is needed for functional analysis. Here, we developed a wide-polarity global metabolomics (G-Met) method, identified HCC biomarkers in human liver samples by high-definition mass spectrometry (HDMS), and demonstrated localization in cryosections using desorption electrospray ionization MS imaging (DESI-MSI) analysis. METHODS: Metabolic profiling of tumor (n = 38) and nontumor (n = 72) regions in human livers of HCC was performed by an ultrahigh-performance liquid chromatography quadrupole time-of-flight MS (UHPLC/QTOFMS) instrument equipped with a mixed-mode column. The HCC biomarker candidates were extracted by multivariate analyses and identified by matching values of the collision cross section and their fragment ions on the mass spectra obtained by HDMS. Cryosections of HCC livers, which included both tumor and nontumor regions, were analyzed by DESI-MSI. RESULTS: From the multivariate analysis, m/z 904.83 and m/z 874.79 were significantly high and low, respectively, in tumor samples and were identified as triglyceride (TG) 16:0/18:1(9Z)/20:1(11Z) and TG 16:0/18:1(9Z)/18:2(9Z,12Z) using the synthetic compounds. The TGs were clearly localized in the tumor or nontumor areas of the cryosection. CONCLUSIONS: Novel biomarkers for HCC were identified by a comprehensive and reproducible G-Met method with HDMS using a mixed-mode column. The combination analysis of UHPLC/QTOFMS and DESI-MSI revealed that the different molecular species of TGs were associated with tumor distribution and were useful for characterizing the progression of tumor cells and discovering prospective biomarkers.
  • Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 2019年09月19日 [査読有り][通常論文]
     
    Background: Triglyceride-rich lipoproteins (TRL) are considered to be independent predictors of atherosclerotic cardiovascular disease. The molecular basis of its atherogenicity is uncertain. Here, we aim to identify molecular species of phosphatidylcholine hydroperoxides (PCOOH) in TRL. For comparison, copper-oxidized TRL were investigated as well. Methods: A fasting EDTA blood sample was collected from 6 healthy human volunteers to isolate two major TRL fractions – very low-density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL) using sequential ultracentrifugation. TRL and plasma samples were studied for PCOOH by liquid chromatography (LC) coupled with Orbitrap mass spectrometry. Results: Twelve molecular species of PCOOH species in TRL and/or plasma were identified using the criterion consisting of 1) high-resolution mass spectrometry (MS) with mass accuracy within 5 ppm, 2) retention time in LC, and 3) fragmentation pattern in MS2 and MS3. PC36:4-OOH was most often detected in VLDL, IDL, and plasma. The ratio of total PCOOH to phosphatidylcholine progressively increased with the duration of oxidation in both VLDL and IDL. Conclusion: This study demonstrated the presence of 12 molecular species of PCOOH in native TRL. The frequent detection of PCOOH in TRL provides a molecular basis of the atherogenicity of TRL. PCOOH in TRL might serve as a molecular basis of the atherogenicity of TRL. Keywords: Lipid hydroperoxide, oxidation, mass spectrometry, phospholipids, very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL)
  • Takayuki Tsukui, Zhen Chen, Hirotoshi Fuda, Takayuki Furukawa, Kotaro Oura, Toshihiro Sakurai, Shu-Ping Hui, Hitoshi Chiba
    Journal of Agricultural and Food Chemistry 2019年09月04日 [査読有り][通常論文]
  • Rapid Communications in Mass Spectrometry 2019年08月14日 [査読有り][通常論文]
  • Yue Wu, Zhen Chen, Wageh S. Darwish, Koh Terada, Hitoshi Chiba, Shu-Ping Hui
    Journal of Agricultural and Food Chemistry 2019年07月10日 [査読有り][通常論文]
  • Zhen Chen, Yue Wu, Rojeet Shrestha, Zijun Gao, Yaoyao Zhao, Yusuke Miura, Akiko Tamakoshi, Hitoshi Chiba, Shu-Ping Hui
    Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 56 2 000456321880139 - 000456321880139 2019年03月23日 [査読有り][通常論文]
  • Yaoyao Zhao, Zhen Chen, Yue Wu, Takayuki Tsukui, Xiaoxiao Ma, Xinrong Zhang, Hitoshi Chiba, Shu-Ping Hui
    Analytical Chemistry 2019年02月 [査読有り][通常論文]
  • Analytical Sciences 2019年 [査読有り][通常論文]
  • Nakamura, Koshi, Hui, Shu-Ping, Ukawa, Shigekazu, Okada, Emiko, Nakagawa, Takafumi, Okabe, Hiroaki, Chen, Zhen, Miura, Yusuke, Chiba, Hitoshi, Tamakoshi, Akiko
    Sleep Medicine 2019年 [査読有り][通常論文]
  • Fumiyoshi Okazaki, Liqing Zang, Hiroko Nakayama, Zhen Chen, Zi-Jun Gao, Hitoshi Chiba, Shu-Ping Hui, Takahiko Aoki, Norihiro Nishimura, Yasuhito Shimada
    Scientific Reports 9 1 2019年01月 [査読有り][通常論文]
  • Zhen Chen, Liqing Zang, Yue Wu, Hiroko Nakayama, Yasuhito Shimada, Rojeet Shrestha, Yaoyao Zhao, Yusuke Miura, Hitoshi Chiba, Shu-Ping Hui, Norihiro Nishimura
    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 34 10 1201 - 1208 2018年10月10日 [査読有り][通常論文]
     
    Type 2 diabetes mellitus is a serious metabolic disorder in the world. Oxidative stress, as a key role on the pathogenesis of diabetes, also results in the oxidation of phospholipids. However, studies on phospholipid oxidation in diabetes, especially directly focusing on oxidized and degraded phospholipid species, are quite limited. In this study, phospholipid profiles of diabetic zebrafish plasma were characterized by LC-HRMS and MS/MS, and the total amounts of each lipid class were compared. Furthermore, the key molecular species as biomarkers in distinguishing control and diabetic samples were investigated by orthogonal partial least squares discriminant analysis. Among the identified 114 phospholipid species in total, there were 11 hydroperoxides, 7 aldehydes, and 19 lysophospholipids found significantly elevated along with the increasing blood glucose, which were known as oxidation or degradation products. Furthermore, lysophosphatidylcholine 20:5 and lysophosphatidylcholine 22:6 were assessed as potential biomarkers in diabetic zebrafish. The current work would not only help to gain further insights into diabetes, but also contribute to find new clinical parameters for the screening of the promising antioxidant agents for its therapies.
  • Sakurai T, Hayasaka T, Sekiguchi H, Satoh H, Chen Z, Chiba H, Hui SP
    Journal of the science of food and agriculture 2018年09月 [査読有り][通常論文]
  • Okabe, Hiroaki, Shimizu, Chikara, Yamamoto, Maiko, Kikuchi, Rei, Minami, Akiko, Chen, Yi-Fan, Imai, Hideyuki, Mizuta, Masahiro, Chen, Zhen, Chiba, Hitoshi, Hui, Shu-Ping
    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 34 9 1043 - 1047 2018年09月 [査読有り][通常論文]
  • Chen, Zhen, Wu, Yue, Shrestha, Rojeet, Gao, Zijun, Zhao, Yaoyao, Miura, Yusuke, Tamakoshi, Akiko, Chiba, Hitoshi, Hui, Shu-Ping
    Annals of clinical biochemistry 4563218801393 - 4563218801393 2018年09月 [査読有り][通常論文]
  • Rojeet Shrestha, Yusuke Miura, Ken-Ichi Hirano, Zhen Chen, Hiroaki Okabe, Hitoshi Chiba, Shu-Ping Hui
    Analytical Sciences 34 5 575 - 582 2018年 [査読有り][通常論文]
     
    Fatty acid (FA) profiling of milk has important applications in human health and nutrition. Conventional methods for the saponification and derivatization of FA are time-consuming and laborious. We aimed to develop a simple, rapid, and economical method for the determination of FA in milk. We applied a beneficial approach of microwave-assisted saponification (MAS) of milk fats and microwave-assisted derivatization (MAD) of FA to its hydrazides, integrated with HPLC-based analysis. The optimal conditions for MAS and MAD were determined. Microwave irradiation significantly reduced the sample preparation time from 80 min in the conventional method to less than 3 min. We used three internal standards for the measurement of short-, medium- and long-chain FA. The proposed method showed satisfactory analytical sensitivity, recovery and reproducibility. There was a significant correlation in the milk FA concentrations between the proposed and conventional methods. Being quick, economic, and convenient, the proposed method for the milk FA measurement can be substitute for the convention method.
  • Zhen Chen, Yue Wu, Yi-Shing Ma, Yuu Kobayashi, Yao-Yao Zhao, Yusuke Miura, Hitoshi Chiba, Shu-Ping Hui
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409 24 5735 - 5745 2017年09月 [査読有り][通常論文]
     
    Cardiolipin (CL) exists as crucial functional phospholipid in mitochondria. The oxidation of CL is concerned with mitochondrial dysfunction and various diseases. As main oxidation products, CL hydroperoxide (CL-OOH) plays a key role in intermediating oxidative reaction. Thus, direct analysis of CL-OOH is of great interest. In the present study, CL and CL-OOH profiles were analyzed in oxidized HepG2 cell lipid via HPLC-Orbitrap MS/MS. Furthermore, the contents of individual molecular species were compared between intact and AAPH-oxidized HepG2 cells. In total, 46 CL and 18 CL-OOH were identified from oxidized cell lipids, while 21 CL and 9 CL-OOH were detected in AAPH-treated cells. Most CL depleted significantly after AAPH inducement, with percentages varying from 8.3% (CL70:7) to 73.7% (CL72:4), depending on fatty acyl composition. While almost all the CL-OOH remarkably increased, among them 68:6-, 72:6-, and 72:7-OOHs were only detected in AAPH-treated cells. CL68:5- and CL68:4-OOH were the most abundant species, while CL70:5-OOH among all the species expressed the highest oxidation percentage of the corresponding CL. Our results showed practical separation, identification, and semi-quantitation of CL-OOH species, which could contribute to approaches to lipidomic analysis of CL and CL-OOH, as well as tracing biomarkers in mitochondrial oxidative stress diagnosis.
  • Xiu-li Zhang, Cong Wang, Zhen Chen, Pei-yu Zhang, Hong-bing Liu
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64 31 6228 - 6232 2016年08月 [査読有り][通常論文]
     
    Knowledge of phytosterol (PS) contents in marine algae is currently lacking compared to those in terrestrial plants. The present studies developed a quantitative H-1 NMR method for the determination of the total PSs in Sargassum. The characteristic proton signal H-3 alpha in PSs was used for quantification, and 2,3,4,5-tetrachloro-nitrobenzene was used as an internal standard. Seaweed samples could be recorded directly after total lipid extraction and saponification. The results showed that the PS contents in Sargassum fusiforme (788.89-2878.67 mg/kg) were significantly higher than those in Sargassum pallidum (585.33-1596.00 mg/kg). The variable contents in both species suggested that fixed raw materials are very important for future research and development. Orthogonal projection to latent structures discriminant analysis was carried out in the spectral region of delta 3.00-6.50 in the H-1 NMR spectrum. S. fusiforme and S. pallidum could be separated well, and the key sterol marker was fucosterol.
  • Zhen Chen, Yibing Xu, Tao Liu, Lining Zhang, Hongbing Liu, Huashi Guan
    MARINE DRUGS 14 4 68  2016年04月 [査読有り][通常論文]
     
    Sargassum seaweeds produce abundant biomass in China and have long been used as herbal medicine and food. Their characteristic fatty acid (FA) profiles and related potential function in promoting cardiovascular health (CVH) have not been systematically investigated. In this study, FA profiles of four medicinal Sargassum were characterized using GC-MS. Principal component analysis was used to discriminate the four medicinal Sargassum, and orthogonal projection to latent structures discriminant analysis was carried out between the two official species HAI ZAO and between the two folk medicine species HAI QIAN. In all of the algae investigated, the major SFA and MUFA were palmitic and stearic acid, respectively, while the major PUFAs were linoleic, arachidonic, and eicosapentaenoic acid. S. fusiforme and S. horneri had higher concentrations of PUFAs. With respect to CVH, all of the studied species, particularly S. fusiforme, exhibited satisfactory levels such as PUFA/SFA ratio and n-6/n-3 ratio. Each species possesses a unique FA profile and is discriminated clearly. Potential key FA markers (between the two Chinese official species, and between the two folk species) are assessed. The study provides characteristic fatty acid profiles of four Chinese medicinal Sargassum and their related potential function in promoting CVH.
  • 张培育, 陈震, 刘淇, 刘红兵
    中国海洋药物 35 4 76  2016年 [査読有り][通常論文]
  • 杨雪, 马爱翠, 陈震, 付志飞, 戚欣, 李静, 管华诗, 刘红兵
    中国海洋大学学报 (自然科学版) 45 9 14  万方数据资源系统 2015年 [査読有り][通常論文]
  • Ai-Cui Ma, Zhen Chen, Tao Wang, Ni Song, Qian Yan, Yu-Chun Fang, Hua-Shi Guan, Hong-Bing Liu
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62 46 11157 - 11162 2014年11月 [査読有り][通常論文]
     
    The chemical composition of monogalactosyldiacylglycerols (MGDGs) from brown alga Sargassum homeri and their inhibitory effects on lipid accumulation were investigated in this study. A total of 10 molecular species of MGDGs were identified using nuclear magnetic resonance, alkaline hydrolysis, gas chromatography flame ionization detector, and high-performance liquid chromatography tandem mass spectrometry methods. Individual molecular species of MGDGs, including (2S)-1-O-myristoyl-2-O-palmitoleoyl-3-O-beta-D-galactopyranosyl-sn-glycerol (1), (2S)-1-O-myristoyl-2-O-linoleyl-3-O-beta-D-galactopyranosyl-sn-glycerol (3), (2S)-1-O-palmitoyl-2-O-linolenoyl-3-O-beta-D-galactopyranosyl-sn-glycerol (5), (2S)-1-O-myristoyl-2-O-oleyl-3-O-beta-D-galactopyranosyl-sn-glycerol (7), (2S)-1-O-palmitoyl-2-O-palmitoleoyl-3-O-beta-D-galactopyranosyl-sn-glycerol (8), (25)-1-O-palmitoyl-2-O-linoleyl-3-O-beta-D-galactopyranosyl-sn-glycerol (9), and (2S)-1-O-palmitoyl-2-O-oleyl-3-O-beta-D-galactopyranosyl-sn-glycerol (10), were then furnished using semi-preparative high-performance liquid chromatography, and their inhibitory effects on triglyceride (TG) accumulation and free fatty acid (FFA) levels in 3T3-L1 adipocytes were evaluated. Compounds 3 and 9 showed inhibitory effects on TG and FFA accumulation, with TG levels of 1.568 +/- 0.2808 and 1.701 +/- 0.1460 mu mol/L and FFA levels of 0.149 +/- 0.0258 and 0.198 +/- 0.0229 mequiv/L, respectively, which were more effective than other compounds. The primary structure activity relationship suggested that linoleyl [18:2(omega-6)] in the sn-2 position played an important role on triglyceride accumulation inhibition.
  • Chen Z, Liu J, Fu Z, Ye C, Zhang R, Song Y, Zhang Y, Li H, Ying H, Liu H
    Journal of agricultural and food chemistry 62 26 6130 - 6137 2014年07月 [査読有り][通常論文]
     
    Dietary phytosterols have been successfully used for lowering cholesterol levels, which correlates with the fact that some phytosterols are able to act as liver X receptor (LXR) agonists. Sargassum fusiforme is an edible marine seaweed well-known for its antiatherosclerotic function in traditional Chinese medicine. In this study, seven phytosterols including fucosterol (1), saringosterol (2), 24-hydroperoxy-24-vinyl-cholesterol (3), 29-hydroperoxy-stigmasta-5,24(28)-dien-3 beta-ol (4), 24-methylene-cholesterol (5), 24-keto-cholesterol (6), and 5 alpha,8 alpha-epidioxyergosta-6,22-dien-3 beta-ol (7) were purified and evaluated for their actions on LXR-mediated transcription using a reporter assay. Among these phytosterols, 2 was the most potent compound in stimulating the transcriptional activities of LXR alpha by (3.81 +/- 0.15)-fold and LXR beta by (14.40 +/- 1.10)-fold, respectively. Two epimers of 2, 24(S)-saringosterol (2a) and 24(R)-saringosterol (2b), were subsequently separated by semipreparative high-performance liquid chromatography. Interestingly, 2a was more potent than 2b in LXR beta-mediated transactivation ((3.50 +/- 0.17)-fold vs (1.63 +/- 0.12)-fold) compared with control. Consistently, 2a induced higher expression levels of LXR target genes including key players in reverse cholesterol transport in six cell lines. These data along with molecular modeling suggested that 2a acts as a selective LXR beta agonist and is a potent natural cholesterol-lowering agent. This study also demonstrated that phytosterols in S. fusiforme contributed to the well-known antiatherosderotic function.

講演・口頭発表等

その他活動・業績

  • 陳 震, 山本 祐輔, 千葉 仁志, 惠 淑萍 JSBMS Letters 45 (2) 18 -24 2020年06月 
    カルジオリピンはミトコンドリア内膜に局在するユニークなリン脂質であり、エネルギー代謝をはじめとする様々なミトコンドリア機能に重要な役割を果たしている。近年、質量分析によるカルジオリピンの分析法が開発され、生物医学的研究へ応用されている。そのことは、カルジオリピンの化学的プロファイル、リモデリング、酸化的変化の理解や、ミトコンドリア機能障害と代謝異常の関係の理解に貢献すると思われる。今後、カルジオリピン分子種のいずれかが健康障害・疾病の潜在的バイオマーカーとして見いだされる可能性もある。この総説では、質量分析により明らかとなったカルジオリピンの化学的プロファイリング、リモデリング、および酸化について焦点をあてる。(著者抄録)
  • ヒト近位尿細管上皮細胞の脂肪滴形成がミトコンドリア固有リン脂質への影響に関する検討
    惠 淑萍, 三浦 佑介, 櫻井 俊宏, 陳 震, 千葉 仁志 日本未病システム学会学術総会抄録集 26回 108 -108 2019年10月 [査読無し][通常論文]
  • 酸化リポタンパク質はHepG2細胞の脂質滴中の脂質過酸化水素の蓄積を誘発する(Oxidized lipoproteins induce accumulation of lipid hydroperoxides in lipid droplets of HepG2 cells)
    Shrestha Rojeet, 陳 震, 桜井 俊宏, 千葉 仁志, 惠 淑萍 臨床化学 48 (Suppl.1) 258 -258 2019年08月 [査読無し][通常論文]
  • ヒト血漿中の脂肪酸に関する派生体化およびLC-MS/MSを用いた定量的組成分析(Quantitative profiling of fatty acids in human plasma by derivatization and LC-MS/MS)
    陳 震, 高 紫君, Wu Yue, ロジート・シュレスタ, 千葉 仁志, 惠 淑萍 臨床化学 48 (Suppl.1) 259 -259 2019年08月 [査読無し][通常論文]
  • 惠 淑萍, 趙 瑤瑤, 陳 震, 呉 げつ, 高 紫君, 張 新栄, 千葉 仁志 JSBMS Letters 44 (Suppl.) 94 -94 2019年08月 [査読無し][通常論文]
  • 陳 震, Yue Wu, Wageh Darwish, 寺田 航, 千葉 仁志, 惠 淑萍 JSBMS Letters 44 (Suppl.) 95 -95 2019年08月 [査読無し][通常論文]
  • 質量分析を用いる肺サーファクタントリン脂質膜に対する環境濃度オゾンの影響に関する研究(Study on the Lipid Monolayer of Lung Surfactant in Low-level Ozone by LC/MS)
    喬 琳, 陳 震, 高田 俊智, 千葉 仁志, 叶 深, 惠 淑萍 JSBMS Letters 44 (Suppl.) 101 -101 2019年08月 [査読無し][通常論文]
  • 櫻井 俊宏, 陳 震, 早坂 孝宏, 千葉 仁志, 惠 淑萍 JSBMS Letters 44 (Suppl.) 99 -99 2019年08月 [査読無し][通常論文]
  • 三浦 佑介, 櫻井 俊宏, 陳 震, 千葉 仁志, 惠 淑萍 臨床化学 48 (Suppl.1) 256 -256 2019年08月 [査読無し][通常論文]
  • 山本 祐輔, 櫻井 俊宏, 三浦 佑介, 陳 震, 千葉 仁志, 惠 淑萍 臨床化学 48 (Suppl.1) 259 -259 2019年08月 [査読無し][通常論文]
  • 環境濃度オゾンによる肺サーファクタントリン脂質膜の酸化に関する研究
    喬 琳, 陳 震, 高田 俊智, 千葉 仁志, 叶 深, 惠 淑萍 脂質生化学研究 61 224 -224 2019年06月 [査読無し][通常論文]
  • 環境濃度オゾンによる肺サーファクタントリン脂質膜の酸化に関する研究
    喬 琳, 陳 震, 高田 俊智, 千葉 仁志, 叶 深, 惠 淑萍 脂質生化学研究 61 224 -224 2019年06月 [査読無し][通常論文]
  • 清水 力, 陳 震, 惠 淑萍, 千葉 仁志 日本内分泌学会雑誌 94 (3) 732 -732 2018年12月 [査読無し][通常論文]
  • 質量分析法による25(OH)D測定法の開発および免疫学的測定法との比較検討
    清水 力, 陳 震, 惠 淑萍, 千葉 仁志 日本内分泌学会雑誌 94 (3) 732 -732 2018年12月 [査読無し][通常論文]
  • 細胞内脂質および過酸化脂質に関する研究
    惠 淑萍, 李 泳翰, 陳 震, ダルウィッシュ・ワギ, 呉 Yue, 趙 瑤瑤, 高 紫君, 千葉 仁志 日本未病システム学会学術総会抄録集 25回 141 -141 2018年10月 [査読無し][通常論文]
  • 辻川諒哉, 三浦佑介, CHEN Zhen, 高田康徳, 千葉仁志, 千葉仁志, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 86 2018年08月25日 [査読無し][通常論文]
  • 山村凌大, CHEN Zhen, WU Yue, GAO Zijun, HUI Shu‐Ping, 千葉仁志, 千葉仁志, 中川貴史, 鵜川重和, 鵜川重和, 中村幸志, 玉腰暁子 JSBMS Letters 43 (Supplement) 84 2018年08月25日 [査読無し][通常論文]
  • WU Yue, UESHIBA Hiroki, CHEN Zhen, SAKAGUCHI Kenichiro, YANAGAWA Yojiro, KATAGIRI Seiji, NAGANO Masashi, CHIBA Hitoshi, CHIBA Hitoshi, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 105 2018年08月25日 [査読無し][通常論文]
  • LI Yonghan, DARWISH Wageh Sobhy, DARWISH Wageh Sobhy, CHEN Zhen, WU Yue, CHIBA Hitoshi, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 150 2018年08月25日 [査読無し][通常論文]
  • GAO Zijun, CHEN Zhen, WU Yue, MIURA Yusuke, CHIBA Hitoshi, CHIBA Hitoshi, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 136 2018年08月25日 [査読無し][通常論文]
  • 櫻井俊宏, 櫻井俊宏, 早坂孝宏, 早坂孝宏, 関口博太, 関口博太, 佐藤浩志, 佐藤浩志, CHEN Zhen, CHEN Zhen, 千葉仁志, 千葉仁志, 千葉仁志, HUI Shu‐Ping, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 147 2018年08月25日 [査読無し][通常論文]
  • QIAO Lin, CHEN Zhen, TAKADA Shunchi, WU Yue, YE Shen, CHIBA Hitoshi, CHIBA Hitoshi, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 151 2018年08月25日 [査読無し][通常論文]
  • CHEN Zhen, WU Yue, MA Yi‐Shing, KOBAYASHI Yuu, ZHAO Yao‐Yao, MIURA Yusuke, CHIBA Hitoshi, CHIBA Hitoshi, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 51 2018年08月25日 [査読無し][通常論文]
  • 津久井隆行, 津久井隆行, GUO Xian‐Hui, CHEN Zhen, HUI Shu‐Ping, 千葉仁志, 千葉仁志 JSBMS Letters 43 (Supplement) 107 2018年08月25日 [査読無し][通常論文]
  • CHEN Zhen, TAMAI Yasuyuki, EGUCHI Akiko, IWASA Motoh, YUE Wu, TAKEI Yoshiyuki, CHIBA Hitoshi, CHIBA Hitoshi, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 106 2018年08月25日 [査読無し][通常論文]
  • ZHAO Yaoyao, CHEN Zhen, WU Yue, GAO Zijun, ZHANG Xinrong, CHIBA Hitoshi, CHIBA Hitoshi, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 103 2018年08月25日 [査読無し][通常論文]
  • 三浦佑介, 加藤颯太, 櫻井俊宏, CHEN Zhen, WU Yue, GAO Zijun, SHRESTHA Rojeet, 中村幸志, 鵜川重和, 鵜川重和, 中川貴史, 玉腰暁子, 千葉仁志, 千葉仁志, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 82 2018年08月25日 [査読無し][通常論文]
  • 渡部勇樹, QIAO Lin, CHEN Zhen, 高田俊智, WU Yue, YE Shen, 千葉仁志, 千葉仁志, 千葉仁志, HUI Shu‐Ping, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 153 2018年08月25日 [査読無し][通常論文]
  • 植芝滉己, 呉ユエ, 陳震, 坂口謙一郎, 柳川洋二郎, 片桐成二, 千葉仁志, 千葉仁志, 惠淑萍, 永野昌志 日本獣医学会学術集会講演要旨集 161st 405 2018年08月21日 [査読無し][通常論文]
  • HepG2細胞内の単一脂肪滴に関するnanoESI-MS直接脂質分析(Direct lipid analysis of single lipid droplets in HepG2 cells by nanoESI-MS)
    趙 瑤瑤, 呉 げつ, 陳 震, 高 紫君, 張 新榮, 千葉 仁志, 惠 淑萍 JSBMS Letters 43 (Suppl.) 102 -102 2018年08月 [査読無し][通常論文]
  • 環境濃度オゾンによるHepG2細胞の酸化に関する研究(Study on the Oxidation of Ambient-level Ozone on HepG2 Cells)
    朱 子健, 喬 琳, 陳 震, 呉 げつ, 千葉 仁志, 惠 淑萍 JSBMS Letters 43 (Suppl.) 152 -152 2018年08月 [査読無し][通常論文]
  • 三浦佑介, 櫻井俊宏, 陳震, 布田博敏, 千葉仁志, 惠淑萍 臨床化学 47 378 2018年07月24日 [査読無し][通常論文]
  • ヒト血清中のブタン酸とカプロン酸のLC-MS/MSを用いた定量法(Determination of butanoic and caproic acid in human serum by LC-MS/MS)
    陳 震, Yue Wu, シュレスタ・ロジート, 三浦 祐介, 趙 瑤瑤, 玉腰 暁子, 千葉 仁志, 惠 淑萍 臨床化学 47 (Suppl.1) 377 -377 2018年07月 [査読無し][通常論文]
  • 惠淑萍, 三浦佑介, 寺田航, 櫻井俊宏, 陳震, 古川貴之, 小林美穂, 清水力, 千葉仁志 腎と脂質研究会プログラム・抄録集 30th 43 2018年 [査読無し][通常論文]
  • 三浦佑介, 櫻井俊宏, 津久井隆行, 陳震, 布田博敏, 千葉仁志, 惠淑萍 臨床化学 46 315 2017年09月01日 [査読無し][通常論文]
  • 三浦佑介, 櫻井俊宏, 津久井隆行, 陳震, 布田博敏, 千葉仁志, 惠淑萍 臨床化学 46 203 2017年09月01日 [査読無し][通常論文]
  • 千葉仁志, 郭先薈, 津久井隆行, 馬逸興, 陳震, 布田博敏, 惠淑萍 臨床化学 46 288 2017年09月01日 [査読無し][通常論文]
  • 加藤颯太, 三浦佑介, 櫻井俊宏, SHRESTHA Rojeet, 陳震, 玉腰暁子, 千葉仁志, 惠淑萍 臨床化学 46 287 2017年09月01日 [査読無し][通常論文]
  • HepG2細胞におけるカルジオリピンとそのヒドロペルオキシド類のLC/MSによる組成分析(Profiling of Cardiolipins and Their Hydroperoxides in HepG2 Cells by LC/MS)
    陳 震, Wu Yue, 馬 逸興, 小林 悠, 趙 瑤瑤, 三浦 佑介, 千葉 仁志, 惠 淑萍 臨床化学 46 (Suppl.1) 284 -284 2017年09月 [査読無し][通常論文]
  • 高血糖ゼブラフィッシュにおけるリン脂質とそのヒドロペルオキシド類のLC/MSによる同時組成分析(Simultaneous profiling of phospholipids and their hydroperoxides in hyperglycemia zebrafish by LC/MS)
    Wu Yue, 陳 震, 臧 黎清, 趙 瑤瑤, 三浦 佑介, 西村 訓弘, 千葉 仁志, 惠 淑萍 臨床化学 46 (Suppl.1) 284 -284 2017年09月 [査読無し][通常論文]
  • 健常日本人被験者における血清総脂肪酸および非エステル化脂肪酸の組成分析法(Serum total and non-esterified fatty acid profiling in healthy Japanese individuals)
    惠 淑萍, Shrestha Rojeet, 三浦 佑介, 陳 震, 玉腰 暁子, 千葉 仁志 臨床化学 46 (Suppl.1) 329 -329 2017年09月 [査読無し][通常論文]
  • LC/MS/MSを用いた過酸化カルジオリピン分子種の一斉分析(Simultaneous analysis of cardiolipin hydroperoxide molecular species by liquid chromatography/tandem mass spectrometry)
    陳 震, 呉 ゆえ, 馬 逸興, 小林 悠, 趙 瑤瑤, 三浦 佑介, 惠 淑萍, 千葉 仁志 JSBMS Letters 42 (Suppl.) 67 -67 2017年08月 [査読無し][通常論文]
  • LC-MS/MSによる近位尿細管上皮細胞の脂質分析
    三浦 佑介, 櫻井 俊宏, 津久井 隆行, 陳 震, 布田 博敏, 千葉 仁志, 惠 淑萍 JSBMS Letters 42 (Suppl.) 110 -110 2017年08月 [査読無し][通常論文]
  • 岡部浩昭, 岡田恵美子, 鵜川重和, 中村幸志, 中川貴史, 玉腰暁子, 菊地玲, 南昭子, 清水力, 陳震, 千葉仁志, 惠淑萍 日本未病システム学会学術総会抄録集 23rd 120 2016年10月15日 [査読無し][通常論文]

受賞

  • 2020年09月 日本臨床化学会北海道支部 若手優秀演題賞
     第54回日本臨床検査医学会北海道支部総会/第30回日本臨床化学会北海道支部例会(合同学会2020)
  • 2019年10月 日本臨床化学会北海道支部 学会賞
     第53回日本臨床検査医学会北海道支部総会/第29回日本臨床化学会北海道支部例会(合同学会2019)
  • 2018年09月 日本医用マススペクトル学会 若手優秀ポスター賞
     第43回日本医用マススペクトル学会年会

共同研究・競争的資金等の研究課題

  • Studies on the association of lipid hydroperoxides and Alzheimer's disease by LC/MS
    日本学術振興会:科研費 若手研究
    研究期間 : 2019年04月 -2021年04月 
    代表者 : 陳震
  • Comparative studies on choline and ethanolamine plasmalogen molecular species for their cytoprotective and anti-lipid oxidative effects
    北海道大学:北大若手研究加速事業
    研究期間 : 2019年07月 -2020年07月 
    代表者 : 陳震

教育活動情報

主要な担当授業

  • 代謝分析化学演習
    開講年度 : 2019年
    課程区分 : 修士課程
    開講学部 : 保健科学院
    キーワード : 電気陰性度、HPLC、質量分析法、LC/MS、Ambient Mass Spectrometry、NMR、細胞培養
  • 医学英語
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 医学英語、英語論文、英文実験プロトコール


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