藤田 知道(フジタ トモミチ)
理学研究院 生物科学部門 形態機能学分野



  • 理学研究院 生物科学部門 形態機能学分野


  • 教授


  • 博士(理学)(東京大学)


J-Global ID


  • コケ植物   細胞極性   不等分裂(非対称分裂)   細胞間コミュニケーション   植物発生   植物生理   成長と環境応答   植物分子細胞生物   進化発生   極限環境ストレス耐性   宇宙生物科学(アストロバイオロジー)   宇宙環境利用   テラフォーミング   


  • ライフサイエンス / 植物分子、生理科学
  • ライフサイエンス / 形態、構造
  • ライフサイエンス / 分子生物学
  • ライフサイエンス / 応用分子細胞生物学
  • その他 / その他 / 宇宙生物科学(アストロバイオロジー)・宇宙環境利用科学


  • 2019年04月 - 現在 北海道大学大学院生命科学院 副学院長
  • 2016年04月 - 現在 北海道大学大学院理学研究院 生物科学部門 教授
  • 2018年04月 - 2019年03月 北海道大学大学院理学研究院 生物科学部門 部門長
  • 2005年07月 - 2016年03月 北海道大学大学院理学研究院 生物科学部門 准教授
  • 1999年10月 - 2005年06月 自然科学研究機構 基礎生物学研究所 生物進化研究部門 助手
  • 1998年05月 - 1999年09月 京都大学大学院理学研究科/名古屋大学大学院理学研究科 ポストドクトラルフェロー
  • 1994年10月 - 1998年04月 Purdue University, Dept. of Biological Sciences ポストドクトラルフェロー
  • 1993年04月 - 1994年09月 国立予防衛生研究所(現、国立感染症研究所) ウイ ルス1部 ポストドクトラルフェロー


  • 1990年04月 - 1993年03月   東京大学   理学系研究科   相関理化学専攻(博士課程)
  • 1988年04月 - 1990年03月   東京大学   理学系研究科   相関理化学専攻(修士課程)


  • international Molecular Moss Science Society (iMOSS)   地衣類研究会   日本宇宙生物科学会   日本蘚苔類学会   日本進化学会   日本分子生物学会   日本植物生理学会   日本植物学会   



  • Marcel Pascal Beier, Chiyo Jinno, Natsumi Noda, Kohei Nakamura, Sumio Sugano, Yutaka Suzuki, Tomomichi Fujita
    Frontiers in Plant Science - Plant Cell Biology 2023年11月01日 [査読有り]
  • Ooi Kock Teh, Prerna Singh, Junling Ren, Lin Tzu Huang, Menaka Ariyarathne, Benjamin Prethiviraj Salamon, Yu Wang, Toshihisa Kotake, Tomomichi Fujita
    Development 149 24 dev200370  2022年12月15日 [査読有り]
    ABSTRACT Arabinogalactan proteins are functionally diverse cell wall structural glycoproteins that have been implicated in cell wall remodeling, although the mechanistic actions remain elusive. Here, we identify and characterize two AGP glycoproteins, SLEEPING BEAUTY (SB) and SB-like (SBL), that negatively regulate the gametophore bud initiation in Physcomitrium patens by dampening cell wall loosening/softening. Disruption of SB and SBL led to accelerated gametophore formation and altered cell wall compositions. The function of SB is glycosylation dependent and genetically connected with the class C auxin response factor (ARF) transcription factors PpARFC1B and PpARFC2. Transcriptomics profiling showed that SB upregulates PpARFC2, which in turn suppresses a range of cell wall-modifying genes that are required for cell wall loosening/softening. We further show that PpARFC2 binds directly to multiple AuxRE motifs on the cis-regulatory sequences of PECTIN METHYLESTERASE to suppress its expression. Hence, our results demonstrate a mechanism by which the SB modulates the strength of intracellular auxin signaling output, which is necessary to fine-tune the timing of gametophore initials formation.
  • Ryohei Yamaura, Daisuke Tamaoki, Hiroyuki Kamachi, Daisuke Yamauchi, Yoshinobu Mineyuki, Kentaro Uesugi, Masato Hoshino, Tomomi Suzuki, Toru Shimazu, Haruo Kasahara, Motoshi Kamada, Yuko T Hanba, Atsushi Kume, Tomomichi Fujita, Ichirou Karahara
    Microscopy (Oxford, England) 2022年08月22日 [査読有り]
    Land plants have two types of shoot-supporting systems, root system and rhizoid system, in vascular plants and bryophytes. However, since the evolutionary origin of the systems are different, how much they exploit common systems or distinct systems to architect their structures are largely unknown. To understand the regulatory mechanism how bryophytes architect rhizoid system responding to environmental factors, we have developed the methodology to visualize and quantitatively analyze the rhizoid system of the moss, Physcomitrium patens in 3D. The rhizoids having the diameter of 21.3 µm on the average were visualized by refraction-contrast X-ray micro-CT using coherent X-ray optics available at synchrotron radiation facility SPring-8. Three types of shape (ring-shape, line, black circle) observed in tomographic slices of specimens embedded in paraffin were confirmed to be the rhizoids by optical and electron microscopy. Comprehensive automatic segmentation of the rhizoids which appeared in different three form types in tomograms was tested by a method using Canny edge detector or machine learning. Accuracy of output images was evaluated by comparing with the manually-segmented ground truth images using measures such as F1 score and IoU, revealing that the automatic segmentation using the machine learning was more effective than that using Canny edge detector. Thus, machine learning-based skeletonized 3D model revealed quite dense distribution of rhizoids. We successfully visualized the moss rhizoid system in 3D for the first time. High resolution refraction-contrast X-ray micro-CT using coherent X-ray optics successfully visualized 3D architecture of rhizoid system of moss, Physcomitrium patens, which is composed of cellular filaments having the diameter of 21.3 µm on the average, for the first time by using machine learning for segmentation.
  • Liang Bao, Junling Ren, Mary Nguyen, Arkadiusz Slawomir Slusarczyk, Julie Thole, Susana Perez Martinez, Jinling Huang, Tomomichi Fujita, Mark Running
    Development 149 12 dev200279  2022年06月 [査読有り]
  • Akihiko Hiroguchi, Kohei Nakamura, Tomomichi Fujita
    Plant Biotechnology 39 1 13 - 17 2022年03月 [査読有り]
  • Liang Bao, Natsumi Inoue, Masaki Ishikawa, Eiji Gotoh, Ooi-Kock Teh, Takeshi Higa, Tomoro Morimoto, Eggie Febrianto Ginanjar, Hirofumi Harashima, Natsumi Noda, Masaaki Watahiki, Yuji Hiwatashi, Masami Sekine, Mitsuyasu Hasebe, Masamitsu Wada, Tomomichi Fujita
    Science Advances 8 4 eabk2116  2022年01月28日 [査読有り]
    Light is a critical signal perceived by plants to adapt their growth rate and direction. Although many signaling components have been studied, how plants respond to constantly fluctuating light remains underexplored. Here, we showed that in the moss Physcomitrium ( Physcomitrella ) patens , the PSTAIRE-type cyclin-dependent kinase PpCDKA is dispensable for growth. Instead, PpCDKA and its homolog in Arabidopsis thaliana control light-induced tropisms and chloroplast movements by probably influencing the cytoskeleton organization independently of the cell cycle. In addition, lower PpCDKA kinase activity was required to elicit light responses relative to cell cycle regulation. Thus, our study suggests that plant CDKAs may have been co-opted to control multiple light responses, and owing to the bistable switch properties of PSTAIRE-type CDKs, the noncanonical functions are widely conserved for eukaryotic environmental adaptation.
  • Eggie Febrianto Ginanjar, Ooi-Kock Teh, Tomomichi Fujita
    The New phytologist 233 2442 - 2457 2021年12月25日 [査読有り]
    Small signalling peptides are key molecules for cell-to-cell communications in plants. The cysteine-rich signalling peptide, rapid alkalinisation factors (RALFs) family are involved in diverse developmental and stress responses and have expanded considerably during land plant evolution, implying neofunctionalisations in the RALF family. However, the ancestral roles of RALFs when land plant first acquired them remain unknown. Here, we functionally characterised two of the three RALFs in bryophyte Physcomitrium patens using loss-of-function mutants, overexpressors as well as fluorescent proteins tagged reporter lines. We showed that PpRALF1 and PpRALF2 have overlapping functions in promoting protonema tip growth and elongation, showing a homologous function as the Arabidopsis RALF1 in promoting root hair tip growth. Although both PpRALFs are secreted to the plasma membrane on which PpRALF1 symmetrically localised, PpRALF2 showed a polarized localisation at the growing tip. Notably, proteolytic cleavage of PpRALF1 is necessary for its function. Our data reveals a possible evolutionary origin of the RALF functions and suggests that functional divergence of RALFs is essential to drive complex morphogenesis and to facilitate other novel processes in land plants.
  • Tomomichi Fujita, Fabien Nogué, Stefan A. Rensing, Daisuke Takezawa, Luis Vidali
    Plant Molecular Biology 107 4-5 209 - 211 2021年11月 [査読無し][招待有り]
  • Hiroki Wakabayashi, Osamu Matsuda, Tomomichi Fujita, Atsushi Kume
    Biological Sciences in Space 35 32 - 40 2021年09月 [査読有り]
  • Satoshi Naramoto, Yuki Hata, Tomomichi Fujita, Junko Kyozuka
    The Plant Cell 38 1 228 - 246 2021年08月30日 [査読有り]
    Abstract Bryophytes are nonvascular spore-forming plants. Unlike in flowering plants, the gametophyte (haploid) generation of bryophytes dominates the sporophyte (diploid) generation. A comparison of bryophytes with flowering plants allows us to answer some fundamental questions raised in evolutionary cell and developmental biology. The moss Physcomitrium patens was the first bryophyte with a sequenced genome. Many cell and developmental studies have been conducted in this species using gene targeting by homologous recombination. The liverwort Marchantia polymorpha has recently emerged as an excellent model system with low genomic redundancy in most of its regulatory pathways. With the development of molecular genetic tools such as efficient genome editing, both P. patens and M. polymorpha have provided many valuable insights. Here, we review these advances with a special focus on polarity formation at the cell and tissue levels. We examine current knowledge regarding the cellular mechanisms of polarized cell elongation and cell division, including symmetric and asymmetric cell division. We also examine the role of polar auxin transport in mosses and liverworts. Finally, we discuss the future of evolutionary cell and developmental biological studies in plants.
  • Shinsa Kameo, Michiki Aso, Ryo Furukawa, Renon Matsumae, Makio Yokono, Tomomichi Fujita, Ayumi Tanaka, Ryouichi Tanaka, Atsushi Takabayashi
    Plant and Cell Physiology 62 2 348 - 355 2021年05月11日 [査読有り]
    Abstract Native polyacrylamide gel electrophoresis (PAGE) is a powerful technique for protein complex separation that retains both their activity and structure. In photosynthetic research, native-PAGE is particularly useful given that photosynthetic complexes are generally large in size, ranging from 200 kD to 1 MD or more. Recently, it has been reported that the addition of amphipol A8-35 to solubilized protein samples improved protein complex stability. In a previous study, we found that amphipol A8-35 could substitute sodium deoxycholate (DOC), a conventional electrophoretic carrier, in clear-native (CN)-PAGE. In this study, we present the optimization of amphipol-based CN-PAGE. We found that the ratio of amphipol A8-35 to α-dodecyl maltoside, a detergent commonly used to solubilize photosynthetic complexes, was critical for resolving photosynthetic machinery in CN-PAGE. In addition, LHCII dissociation from PSII–LHCII was effectively prevented by amphipol-based CN-PAGE compared with that of DOC-based CN-PAGE. Our data strongly suggest that majority of the PSII–LHCII in vivo forms C2S2M2 at least in Arabidopsis and Physcomitrella. The other forms might appear owing to the dissociation of LHCII from PSII during sample preparation and electrophoresis, which could be prevented by the addition of amphipol A8-35 after solubilization from thylakoid membranes. These results suggest that amphipol-based CN-PAGE may be a better alternative to DOC-based CN-PAGE for the study of labile protein complexes.
  • Atsushi Kume, Hiroyuki Kamachi, Yusuke Onoda, Yuko T. Hanba, Yuji Hiwatashi, Ichirou Karahara, Tomomichi Fujita
    Plant Molecular Biology 107 4-5 279 - 291 2021年04月14日 [査読有り]
    Plants have evolved and grown under the selection pressure of gravitational force at 1 g on Earth. In response to this selection pressure, plants have acquired gravitropism to sense gravity and change their growth direction. In addition, plants also adjust their morphogenesis in response to different gravitational forces in a phenomenon known as gravity resistance. However, the gravity resistance phenomenon in plants is poorly understood due to the prevalence of 1 g gravitational force on Earth: not only it is difficult to culture plants at gravity > 1 g(hypergravity) for a long period of time but it is also impossible to create a < 1 genvironment (mu g, micro g) on Earth without specialized facilities. Despite these technical challenges, it is important to understand how plants grow in different gravity conditions in order to understand land plant adaptation to the 1 g environment or for outer space exploration. To address this, we have developed a centrifugal device for a prolonged duration of plant culture in hypergravity conditions, and a project to grow plants under the mu g environment in the International Space Station is also underway. Our plant material of choice is Physcomitrium (Physcomitrella) patens, one of the pioneer plants on land and a model bryophyte often used in plant biology. In this review, we summarize our latest findings regarding P. patens growth response to hypergravity, with reference to our on-going "Space moss" project. In our ground-based hypergravity experiments, we analyzed the morphological and physiological changes and found unexpected increments of chloroplast size and photosynthesis rate, which might underlie the enhancement of growth and increase in the number of gametophores and rhizoids. We further discussed our approaches at the cellular level and compare the gravity resistance in mosses and that in angiosperms. Finally, we highlight the advantages and perspectives from the space experiments and conclude that research with bryophytes is beneficial to comprehensively and precisely understand gravitational responses in plants.
  • Kensuke Kawade, Gorou Horiguchi, Yuu Hirose, Akira Oikawa, Masami Yokota Hirai, Kazuki Saito, Tomomichi Fujita, Hirokazu Tsukaya
    Cell Reports 32 10 108127 - 108127 2020年09月 [査読有り]
    Shoot formation is accompanied by active cell proliferation and expansion, requiring that metabolic state adapts to developmental control. Despite the importance of such metabolic reprogramming, it remains unclear how development and metabolism are integrated. Here, we show that disruption of ANGUSTIFOLIA3 orthologs (PpAN3s) compromises gametophore shoot formation in the moss Physcomitrium patens due to defective cell proliferation and expansion. Trans-omics analysis reveals that the downstream activity of PpAN3 is linked to arginine metabolism. Elevating arginine level by chemical treatment leads to stunted gametophores and causes Ppan3 mutant-like transcriptional changes in the wild-type plant. Furthermore, ectopic expression of AtAN3 from Arabidopsis thaliana ameliorates the defective arginine metabolism and promotes gametophore formation in Ppan3 mutants. Together, these findings indicate that arginine metabolism is a key pathway associated with gametophore formation and provide evolutionary insights into the establishment of the shoot system in land plants through the integration of developmental and metabolic processes.
  • Thi Huong Do, Prapaporn Pongthai, Menaka Ariyarathne, Ooi-Kock Teh, Tomomichi Fujita
    Journal of Plant Research 133 4 537 - 548 2020年07月 [査読有り]
  • Takumi Tomoi, Kensuke Kawade, Munenori Kitagawa, Yoichi Sakata, Hirokazu Tsukaya, Tomomichi Fujita
    Plant and Cell Physiology 61 5 942 - 956 2020年05月01日 [査読有り]
    Abstract Cell-to-cell communication is tightly regulated in response to environmental stimuli in plants. We previously used a photoconvertible fluorescent protein Dendra2 as a model reporter to study this process. This experiment revealed that macromolecular trafficking between protonemal cells in Physcomitrella patens is suppressed in response to abscisic acid (ABA). However, it remains unknown which ABA signaling components contribute to this suppression and how. Here, we show that ABA signaling components SUCROSE NON-FERMENTING 1-RELATED PROTEIN KINASE 2 (PpSnRK2) and ABA INSENSITIVE 3 (PpABI3) play roles as an essential and promotive factor, respectively, in regulating ABA-induced suppression of Dendra2 diffusion between cells (ASD). Our quantitative imaging analysis revealed that disruption of PpSnRK2 resulted in defective ASD onset itself, whereas disruption of PpABI3 caused an 81-min delay in the initiation of ASD. Live-cell imaging of callose deposition using aniline blue staining showed that, despite this onset delay, callose deposition on cross walls remained constant in the PpABI3 disruptant, suggesting that PpABI3 facilitates ASD in a callose-independent manner. Given that ABA is an important phytohormone to cope with abiotic stresses, we further explored cellular physiological responses. We found that the acquisition of salt stress tolerance is promoted by PpABI3 in a quantitative manner similar to ASD. Our results suggest that PpABI3-mediated ABA signaling may effectively coordinate cell-to-cell communication during the acquisition of salt stress tolerance. This study will accelerate the quantitative study for ABA signaling mechanism and function in response to various abiotic stresses.
  • Furukawa, R, Aso M, Fujita, T, Akimoto, S, Tanaka, R, Tanaka, A, Yokono, M, Takabayashi, A
    Journal of Plant Research 132 6 867 - 880 2019年11月 [査読有り][通常論文]
  • Yokono, M, Takabayashi, A, Kishimoto, J, Fujita, T, Iwai, M, Murakami, A, Akimoto, S, Tanaka, A
    Plant and Cell Physiology 60 5 1098 - 1108 2019年05月 [査読有り][通常論文]
  • Munenori Kitagawa, Takumi Tomoi, Tomoki Fukushima, Yoichi Sakata, Mayuko Sato, Kiminori Toyooka, Tomomichi Fujita, Hitoshi Sakakibara
    Plant and Cell Physiology 60 4 738 - 751 2019年04月01日 [査読有り][通常論文]
    In multi-cellular organisms, cell-to-cell communication is crucial for adapting to changes in the surrounding environment. In plants, plasmodesmata (PD) provide a unique pathway for cell-to-cell communication. PD interconnect most cells and generate a cytoplasmic continuum, allowing the trafficking of various micro- and macromolecules between cells. This molecular trafficking through PD is dynamically regulated by altering PD permeability dependent on environmental changes, thereby leading to an appropriate response to various stresses; however, how PD permeability is dynamically regulated is still largely unknown. Moreover, studies on the regulation of PD permeability have been conducted primarily in a limited number of angiosperms. Here, we studied the regulation of PD permeability in the moss Physcomitrella patens and report that molecular trafficking through PD is rapidly and reversibly restricted by abscisic acid (ABA). Since ABA plays a key role in various stress responses in the moss, PD permeability can be controlled by ABA to adapt to surrounding environmental changes. This ABA-dependent restriction of PD trafficking correlates with a reduction in PD pore size. Furthermore, we also found that the rate of macromolecular trafficking is higher in an ABA-synthesis defective mutant, suggesting that the endogenous level of ABA is also important for PD-mediated macromolecular trafficking. Thus, our study provides compelling evidence that P. patens exploits ABA as one of the key regulators of PD function.
  • Takemura K, Kamachi H, Kume A, Fujita T, Karahara I, Hanba YT
    Journal of Plant Research 131 5 887 - 887 2018年09月 [査読有り][通常論文]
    The original article can be found online.
  • Pierre-François Perroud, Fabian B. Haas, Manuel Hiss, Kristian K. Ullrich, Alessandro Alboresi, Mojgan Amirebrahimi, Kerrie Barry, Roberto Bassi, Sandrine Bonhomme, Haodong Chen, Juliet C. Coates, Tomomichi Fujita, Anouchka Guyon-Debast, Daniel Lang, Junyan Lin, Anna Lipzen, Fabien Nogué, Melvin J. Oliver, Inés Ponce de León, Ralph S. Quatrano, Catherine Rameau, Bernd Reiss, Ralf Reski, Mariana Ricca, Younousse Saidi, Ning Sun, Péter Szövényi, Avinash Sreedasyam, Jane Grimwood, Gary Stacey, Jeremy Schmutz, Stefan A. Rensing
    Plant Journal 95 1 168 - 182 2018年07月01日 [査読有り][通常論文]
    High-throughput RNA sequencing (RNA-seq) has recently become the method of choice to define and analyze transcriptomes. For the model moss Physcomitrella patens, although this method has been used to help analyze specific perturbations, no overall reference dataset has yet been established. In the framework of the Gene Atlas project, the Joint Genome Institute selected P. patens as a flagship genome, opening the way to generate the first comprehensive transcriptome dataset for this moss. The first round of sequencing described here is composed of 99 independent libraries spanning 34 different developmental stages and conditions. Upon dataset quality control and processing through read mapping, 28 509 of the 34 361 v3.3 gene models (83%) were detected to be expressed across the samples. Differentially expressed genes (DEGs) were calculated across the dataset to permit perturbation comparisons between conditions. The analysis of the three most distinct and abundant P. patens growth stages – protonema, gametophore and sporophyte – allowed us to define both general transcriptional patterns and stage-specific transcripts. As an example of variation of physico-chemical growth conditions, we detail here the impact of ammonium supplementation under standard growth conditions on the protonemal transcriptome. Finally, the cooperative nature of this project allowed us to analyze inter-laboratory variation, as 13 different laboratories around the world provided samples. We compare differences in the replication of experiments in a single laboratory and between different laboratories.
  • 緑色植物のPSI-PSII複合体
    横野 牧生, 高林 厚史, 岸本 純子, 藤田 知道, 岩井 優和, 村上 明男, 秋本 誠志, 田中 歩
    光合成研究 28 1 15 - 19 2018年04月 [査読有り][通常論文]
  • Kaori Takemura, Rina Watanabe, Ryuji Kameishi, Naoya Sakaguchi, Hiroyuki Kamachi, Atsushi Kume, Ichirou Karahara, Yuko T. Hanba, Tomomichi Fujita
    MICROGRAVITY SCIENCE AND TECHNOLOGY 29 6 467 - 473 2017年12月 [査読有り][通常論文]
    The photosynthetic and anatomical responses of bryophytes to changes in gravity will provide crucial information for estimating how these plant traits evolved to adapt to changes in gravity in land plant history. We performed long-term hypergravity experiments at 10g for 4 and 8 weeks using the moss Physcomitrella patens with two centrifuges equipped with lighting systems that enable long-term plant growth under hypergravity with irradiance. The aims of this study are (1) to quantify changes in the anatomy and morphology of P. patens, and (2) to analyze the post-effects of hypergravity on photosynthesis by P. patens in relation to these changes. We measured photosynthesis by P. patens for a population of gametophores (e.g., canopy) in Petri dishes and plant culture boxes. Gametophore numbers increased by 9% for a canopy of P. patens, with 24-27% increases in chloroplast sizes (diameter and thickness) in leaf cells. In a canopy of P. patens, the area-based photosynthesis rate (A (canopy)) was increased by 57% at 10g. The increase observed in A (canopy) was associated with greater plant numbers and chloroplast sizes, both of which involved enhanced CO2 diffusion from the atmosphere to chloroplasts in the canopies of P. patens. These results suggest that changes in gravity are important environmental stimuli to induce changes in plant growth and photosynthesis by P. patens, in which an alteration in chloroplast size is one of the key traits. We are now planning an ISS experiment to investigate the responses of P. patens to microgravity.
  • Takao Yokota, Toshiyuki Ohnishi, Kyomi Shibata, Masashi Asahina, Takahito Nomura, Tomomichi Fujita, Kimitsune Ishizaki, Takayuki Kohchi
    PHYTOCHEMISTRY 136 46 - 55 2017年04月 [査読有り][通常論文]
    Endogenous brassinosteroids (BRs) in non-flowering land plants were analyzed. BRs were found in a liverwort (Marchantia polymorpha), a moss (Physcomitrella patens), lycophytes (Selaginella moellendorffii and S. uncinata) and 13 fern species. A biologically active BR, castasterone (CS), was identified in most of these non-flowering plants but another biologically active BR, brassinolide, was not. It may be distinctive that levels of CS in non-flowering plants were orders of magnitude lower than those in flowering plants. 22-Hydroxycampesterol and its metabolites were identified in most of the non-flowering plants suggesting that the biosynthesis of BRs via 22-hydroxylation of campesterol occurs as in flowering plants. Phylogenetic analyses indicated that M. polymorpha, P. patens and S. moellendorffii have cytochrome P450s in the CYP85 clans which harbors BR biosynthesis enzymes, although the P450 profiles are simpler as compared with Arabidopsis and rice. Furthermore, these basal land plants were found to have multiple P450s in the CYP72 clan which harbors enzymes to catabolize BRs. These findings indicate that green plants were able to synthesize and inactivate BRs from the land-transition stage. (C) 2016 Elsevier Ltd. All rights reserved.
  • Kenji Fukushima, Xiaodong Fang, David Alvarez-Ponce, Huimin Cai, Lorenzo Carretero-Paulet, Cui Chen, Tien-Hao Chang, Kimberly M. Farr, Tomomichi Fujita, Yuji Hiwatashi, Yoshikazu Hoshi, Takamasa Imai, Masahiro Kasahara, Pablo Librado, Likai Mao, Hitoshi Mori, Tomoaki Nishiyama, Masafumi Nozawa, Gergo Palfalvi, Stephen T. Pollard, Julio Rozas, Alejandro Sanchez-Gracia, David Sankoff, Tomoko F. Shibata, Shuji Shigenobu, Naomi Sumikawa, Taketoshi Uzawa, Meiying Xie, Chunfang Zheng, David D. Pollock, Victor A. Albert, Shuaicheng Li, Mitsuyasu Hasebe
    NATURE ECOLOGY & EVOLUTION 1 3 0059  2017年03月 [査読有り][通常論文]
    Carnivorous plants exploit animals as a nutritional source and have inspired long-standing questions about the origin and evolution of carnivory-related traits. To investigate the molecular bases of carnivory, we sequenced the genome of the heterophyllous pitcher plant Cephalotus follicularis, in which we succeeded in regulating the developmental switch between carnivorous and non-carnivorous leaves. Transcriptome comparison of the two leaf types and gene repertoire analysis identified genetic changes associated with prey attraction, capture, digestion and nutrient absorption. Analysis of digestive fluid proteins from C. follicularis and three other carnivorous plants with independent carnivorous origins revealed repeated co-options of stress-responsive protein lineages coupled with convergent amino acid substitutions to acquire digestive physiology. These results imply constraints on the available routes to evolve plant carnivory.
  • Kaori Takemura, Hiroyuki Kamachi, Atsushi Kume, Tomomichi Fujita, Ichirou Karahara, Yuko T. Hanba
    JOURNAL OF PLANT RESEARCH 130 1 181 - 192 2017年01月 [査読有り][通常論文]
    The physiological and anatomical responses of bryophytes to altered gravity conditions will provide crucial information for estimating how plant physiological traits have evolved to adapt to significant increases in the effects of gravity in land plant history. We quantified changes in plant growth and photosynthesis in the model plant of mosses, Physcomitrella patens, grown under a hypergravity environment for 25 days or 8 weeks using a custom-built centrifuge equipped with a lighting system. This is the first study to examine the response of bryophytes to hypergravity conditions. Canopy-based plant growth was significantly increased at 10xg, and was strongly affected by increases in plant numbers. Rhizoid lengths for individual gametophores were significantly increased at 10xg. Chloroplast diameters (major axis) and thicknesses (minor axis) in the leaves of P. patens were also increased at 10xg. The area-based photosynthesis rate of P. patens was also enhanced at 10xg. Increases in shoot numbers and chloroplast sizes may elevate the area-based photosynthesis rate under hypergravity conditions. We observed a decrease in leaf cell wall thickness under hypergravity conditions, which is in contrast to previous findings obtained using angiosperms. Since mosses including P. patens live in dense populations, an increase in canopy-based plant numbers may be effective to enhance the toughness of the population, and, thus, represents an effective adaptation strategy to a hypergravity environment for P. patens.
  • Atsushi Takabayashi, Saeka Takabayashi, Kaori Takahashi, Mai Watanabe, Hiroko Uchida, Akio Murakami, Tomomichi Fujita, Masahiko Ikeuchi, Ayumi Tanaka
    PLANT AND CELL PHYSIOLOGY 58 1 2017年01月 [査読有り][通常論文]
    The identification of protein complexes is important for the understanding of protein structure and function and the regulation of cellular processes. We used blue-native PAGE and tandemmass spectrometry to identify protein complexes systematically, and built a web database, the protein co-migration database (PCoM-DB, http://pcomdb.lowtem.hokudai.ac.jp/proteins/top), to provide prediction tools for protein complexes. PCoM-DB provides migration profiles for any given protein of interest, and allows users to compare them with migration profiles of other proteins, showing the oligomeric states of proteins and thus identifying potential interaction partners. The initial version of PCoM-DB (launched in January 2013) included protein complex data for Synechocystis whole cells and Arabidopsis thaliana thylakoid membranes. Here we report PCoM-DB version 2.0, which includes new data sets and analytical tools. Additional data are included from whole cells of the pelagic marine picocya-nobacterium Prochlorococcus marinus, the thermophilic cyanobacterium Thermosynechococcus elongatus, the unicellular green alga Chlamydomonas reinhardtii and the bryophyte Physcomitrella patens. The Arabidopsis protein data now include data for intact mitochondria, intact chloroplasts, chloroplast stroma and chloroplast envelopes. The new tools comprise a multiple-protein search form and a heat map viewer for protein migration profiles. Users can compare migration profiles of a protein of interest among different organelles or compare migration profiles among different proteins within the same sample. For Arabidopsis proteins, users can compare migration profiles of a protein of interest with putative homologous proteins from non-Arabidopsis organisms. The updated PCoM-DB will help researchers find novel protein complexes and estimate their evolutionary changes in the green lineage.
  • Mori, A, Kamachi, H, Karahara, I, Kume, A, Hanba, Y.-T, Takemura, K, Fujita, T
    Biological Sciences in Space 31 9 - 13 2017年 [査読有り][通常論文]
  • Liang Bao, Kotaro T. Yamamoto, Tomomichi Fujita
    Plant Signaling and Behavior 10 3 2015年04月07日 [査読有り][通常論文]
    Shoot phototropism enables plants to position their photosynthetic organs in favorable light conditions and thus benefits growth and metabolism in land plants. To understand the evolution of this response, we established an experimental system to study phototropism in gametophores of the moss Physcomitrella patens. The phototropic response of gametophores occurs slowly a clear response takes place more than 24 hours after the onset of unilateral light irradiation, likely due to the slow growth rate of gametophores. We also found that red and far-red light can induce phototropism, with blue light being less effective. These results suggest that plants used a broad range of light wavelengths as phototropic signals during the early evolution of land plants.
  • Munenori Kitagawa, Tomomichi Fujita
    JOURNAL OF PLANT RESEARCH 128 1 63 - 72 2015年01月 [査読有り][通常論文]
    Plant growth, development, and environmental responses require the proper regulation of intercellular movement of signals and nutrients. For this, plants have specialized cytoplasmic channels, the plasmodesmata (PD), which allow the symplasmic movement of micro- and macromolecules between neighboring cells. Internal and external signals spatio-temporally regulate the movement of molecules through the PD to control plant development and environmental responses. Although some aspects of targeted movement of molecules have been revealed, the mechanisms of non-targeted, diffusible flow of molecules through PD, and its regulation and function, remain poorly understood, particularly at the cellular level. Previously, we developed a system to quantitatively analyze non-targeted movement of a photoconvertible fluorescent protein, Dendra2, at the single-cell level in the filamentous protonemata tissue of the moss Physcomitrella patens. In protonemata, one-dimensional intercellular communication can be easily observed and quantitatively analyzed at the cellular level. In this review, we describe how protonemata and leaves of P. patens can be used to study symplasmic movement through PD, and discuss how this system can help improve our understanding of PD regulation and function in development and environmental responses in plants.
  • Tomomichi Fujita
    JOURNAL OF PLANT RESEARCH 128 1 3 - 5 2015年01月 [査読有り][通常論文]
  • 藤田拓矢, 高林厚史, 田中歩, 藤田知道
    せん苔類研究 11 3 92  2014年12月 [査読無し][通常論文]
  • Kanako Ito, Junling Ren, Tomomichi Fujita
    GENE 544 2 241 - 247 2014年07月 [査読有り][通常論文]
    Cell polarity is fundamentally important to growth and development in higher plants, from pollen tubes to root hairs. Basal land plants (mosses and ferns) also have cell polarity, developing protonemal apical cells that show polar tip growth. Flowering plants have a distinct group of Rho GTPases that regulate polarity in polarized cell growth. Rop/RAC signaling module components have been identified in non-flowering plants, but their roles remain unclear. To understand the importance and evolution of Rop/RAC signaling in polarity regulation in land plants, we examined the functions of PpRop and PpRopGEF in protonemal apical cells of the moss Physcomitrella patens. Inducible overexpression of PpRop2 or PpRopGEF3 caused depolarized growth of tip-growing apical cells. PpRop2 overexpression also caused aberrant cross wall formation. Fluorescent protein-tagged PpRop2 localized to the plasma membrane, including the cross wall membrane, and fluorescent-tagged PpRopGEF3 showed polarized localization to the tip region in apical cells. Thus, our results suggest common functions of PpRop and PpRopGEF in the tip-growing apical cells and the importance of a conserved Rop/RAC signaling module in the control of cell polarity in land plants. (C) 2014 Elsevier B.V. All rights reserved.
  • Munenori Kitagawa, Tomomichi Fujita
    JOURNAL OF PLANT RESEARCH 126 4 577 - 585 2013年07月 [査読有り][通常論文]
    Cell-to-cell transport of molecules in plants must be properly regulated for plant growth and development. One specialized mechanism that plants have evolved involves transport through plasmodesmata (PD), but when and how transport of molecules via PD is regulated among individual cells remains largely unknown, particularly at the single-cell level. Here, we developed a tool for quantitatively analyzing cell-to-cell transport via PD at a single-cell level using protonemata of Physcomitrella patens and a photoconvertible fluorescent protein, Dendra2. In the filamentous protonemal tissues, one-dimensional intercellular communication can be observed easily. Using this system, we found that Dendra2 was directionally transported toward the apex of the growing protonemata. However, this directional transport could be eliminated by incubation in the dark or treatment with a metabolic inhibitor. Thus, we propose that directional transport of macromolecules can occur via PD in moss protonemata, and may be affected by the photosynthetic and metabolic activity of cells.
  • Jo Ann Banks, Tomoaki Nishiyama, Mitsuyasu Hasebe, John L. Bowman, Michael Gribskov, Claude dePamphilis, Victor A. Albert, Naoki Aono, Tsuyoshi Aoyama, Barbara A. Ambrose, Neil W. Ashton, Michael J. Axtell, Elizabeth Barker, Michael S. Barker, Jeffrey L. Bennetzen, Nicholas D. Bonawitz, Clint Chapple, Chaoyang Cheng, Luiz Gustavo Guedes Correa, Michael Dacre, Jeremy DeBarry, Ingo Dreyer, Marek Elias, Eric M. Engstrom, Mark Estelle, Liang Feng, Cedric Finet, Sandra K. Floyd, Wolf B. Frommer, Tomomichi Fujita, Lydia Gramzow, Michael Gutensohn, Jesper Harholt, Mitsuru Hattori, Alexander Heyl, Tadayoshi Hirai, Yuji Hiwatashi, Masaki Ishikawa, Mineko Iwata, Kenneth G. Karol, Barbara Koehler, Uener Kolukisaoglu, Minoru Kubo, Tetsuya Kurata, Sylvie Lalonde, Kejie Li, Ying Li, Amy Litt, Eric Lyons, Gerard Manning, Takeshi Maruyama, Todd P. Michael, Koji Mikami, Saori Miyazaki, Shin-ichi Morinaga, Takashi Murata, Bernd Mueller-Roeber, David R. Nelson, Mari Obara, Yasuko Oguri, Richard G. Olmstead, Naoko Onodera, Bent Larsen Petersen, Birgit Pils, Michael Prigge, Stefan A. Rensing, Diego Mauricio Riano-Pachon, Alison W. Roberts, Yoshikatsu Sato, Henrik Vibe Scheller, Burkhard Schulz, Christian Schulz, Eugene V. Shakirov, Nakako Shibagaki, Naoki Shinohara, Dorothy E. Shippen, Iben Sorensen, Ryo Sotooka, Nagisa Sugimoto, Mamoru Sugita, Naomi Sumikawa, Milos Tanurdzic, Guenter Theissen, Peter Ulvskov, Sachiko Wakazuki, Jing-Ke Weng, William W. G. T. Willats, Daniel Wipf, Paul G. Wolf, Lixing Yang, Andreas D. Zimmer, Qihui Zhu, Therese Mitros, Uffe Hellsten, Dominique Loque, Robert Otillar, Asaf Salamov, Jeremy Schmutz, Harris Shapiro, Erika Lindquist, Susan Lucas, Daniel Rokhsar, Igor V. Grigoriev
    SCIENCE 332 6032 960 - 963 2011年05月 [査読有り][通常論文]
    Vascular plants appeared similar to 410 million years ago, then diverged into several lineages of which only two survive: the euphyllophytes (ferns and seed plants) and the lycophytes. We report here the genome sequence of the lycophyte Selaginella moellendorffii (Selaginella), the first nonseed vascular plant genome reported. By comparing gene content in evolutionarily diverse taxa, we found that the transition from a gametophyte- to a sporophyte-dominated life cycle required far fewer new genes than the transition from a nonseed vascular to a flowering plant, whereas secondary metabolic genes expanded extensively and in parallel in the lycophyte and angiosperm lineages. Selaginella differs in posttranscriptional gene regulation, including small RNA regulation of repetitive elements, an absence of the trans-acting small interfering RNA pathway, and extensive RNA editing of organellar genes.
  • Yoshihisa Oda, Aiko Hirata, Toshio Sano, Tomomichi Fujita, Yuji Hiwatashi, Yoshikatsu Sato, Akeo Kadota, Mitsuyasu Hasebe, Seiichiro Hasezawa
    PLANT AND CELL PHYSIOLOGY 50 4 855 - 868 2009年04月 [査読有り][通常論文]
    Eukaryotic cells have developed several essential membrane components. In flowering plants, appropriate structures and distributions of the major membrane components are predominantly regulated by actin microfilaments. In this study, we have focused on the regulatory mechanism of vacuolar structures in the moss, Physcomitrella patens. The high ability of P. patens to undergo homologous recombination enabled us stably to express green fluorescent protein (GFP) or red fluorescent protein (RFP) fusion proteins, and the simple body structure of P. patens enabled us to perform detailed visualization of the intracellular vacuolar and cytoskeletal structures. Three-dimensional analysis and high-speed time-lapse observations revealed surprisingly complex structures and dynamics of the vacuole, with inner sheets and tubular protrusions, and frequent rearrangements by separation and fusion of the membranes. Depolymerization of microtubules dramatically affected these structures and movements. Dual observation of microtubules and vacuolar membranes revealed that microtubules induced tubular protrusions and cytoplasmic strands of the vacuoles, indicative of interactions between microtubules and vacuolar membranes. These results demonstrate a novel function of microtubules in maintaining the distribution of the vacuole and suggest a functional divergence of cytoskeletal functions in land plant evolution.
  • Convergences and divergences in polar auxin transport and shoot development in land plant evolution
    Fujita, T, Hasebe, M
    Plant Signaling & Behavior 4 313 - 315 2009年 [査読有り][通常論文]
  • Yuji Hiwatashi, Mari Obara, Yoshikatsu Sato, Tomomichi Fujita, Takashi Murata, Mitsuyasu Hasebe
    PLANT CELL 20 11 3094 - 3106 2008年11月 [査読有り][通常論文]
    Microtubules form arrays with parallel and antiparallel bundles and function in various cellular processes, including subcellular transport and cell division. The antiparallel bundles in phragmoplasts, plant-unique microtubule arrays, are mostly unexplored and potentially offer new cellular insights. Here, we report that the Physcomitrella patens kinesins KINID1a and KINID1b (for kinesin for interdigitated microtubules 1a and 1b), which are specific to land plants and orthologous to Arabidopsis thaliana PAKRP2, are novel factors indispensable for the generation of interdigitated antiparallel microtubules in the phragmoplasts of the moss P. patens. KINID1a and KINID1b are predominantly localized to the putative interdigitated parts of antiparallel microtubules. This interdigitation disappeared in double-deletion mutants of both genes, indicating that both KINID1a and 1b are indispensable for interdigitation of the antiparallel microtubule array. Furthermore, cell plates formed by these phragmoplasts did not reach the plasma membrane in; 20% of the mutant cells examined. We observed that in the double-deletion mutant lines, chloroplasts remained between the plasma membrane and the expanding margins of the cell plate, while chloroplasts were absent from the margins of the cell plates in the wild type. This suggests that the kinesins, the antiparallel microtubule bundles with interdigitation, or both are necessary for proper progression of cell wall expansion.
  • Takayuki Inouye, Masaki Odahara, Tomomichi Fujita, Mitsuyasu Hasebe, Yasuhiko Sekine
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 72 5 1340 - 1347 2008年05月 [査読有り][通常論文]
    RecA protein is widespread in bacteria, and it plays a crucial role in homologous recombination. We have identified two bacterial-type recA gene homologs (PprecA1, PprecA2) in the cDNA library of the moss Physcomitrella patens. N-terminal fusion of the putative organellar targeting sequence of PpRecA2 to the green fluorescent protein (GFP) caused a targeting of PpRecA2 to the chloroplasts. Mutational analysis showed that the first AUG codon acts as initiation codon. Fusion of the full-length PpRecA2 to GFP caused the formation of foci that were colocalized with chloroplast nucleoids. The amounts of PprecA2 mRNA and protein in the cells were increased by treatment, with DNA damaging agents. PprecA2 partially complemented the recA mutation in Escherichia coli. These results suggest the involvement of PpRecA2 in the repair of chloroplast DNA.
  • Tomomichi Fujita, Hisako Sakaguchi, Yuji Hiwatashi, Steven J. Wagstaff, Motomi Ito, Hironori Deguchi, Toshiyuki Sato, Mitsuyasu Hasebe
    EVOLUTION & DEVELOPMENT 10 2 176 - 186 2008年03月 [査読有り][通常論文]
    The shoot is a repeated structure made up of stems and leaves and is the basic body plan in land plants. Vascular plants form a shoot in the diploid generation, whereas nonvascular plants such as mosses form a shoot in the haploid generation. It is not clear whether all land plants use similar molecular mechanisms in shoot development or how the genetic networks for shoot development evolved. The control of auxin distribution, especially by polar auxin transport, is essential for shoot development in flowering plants. We did not detect polar auxin transport in the gametophytic shoots of several mosses, but did detect it in the sporophytes of mosses without shoot structure. Treatment with auxin transport inhibitors resulted in abnormal embryo development, as in flowering plants, but did not cause any morphological changes in the haploid shoots. We fused the soybean auxin-inducible promoter GH3 with a GUS reporter gene and used it to indirectly detect auxin distribution in the moss Physcomitrella patens. An auxin transport inhibitor NPA did not cause any changes in the putative distribution of auxin in the haploid shoot. These results indicate that polar auxin transport is not involved in haploid shoot development in mosses and that shoots in vascular plants and mosses are most likely regulated differently during development.
  • Stefan A. Rensing, Daniel Lang, Andreas D. Zimmer, Astrid Terry, Asaf Salamov, Harris Shapiro, Tomoaki Nishiyama, Pierre-Francois Perroud, Erika A. Lindquist, Yasuko Kamisugi, Takako Tanahashi, Keiko Sakakibara, Tomomichi Fujita, Kazuko Oishi, Tadasu Shin-I, Yoko Kuroki, Atsushi Toyoda, Yutaka Suzuki, Shin-ichi Hashimoto, Kazuo Yamaguchi, Sumio Sugano, Yuji Kohara, Asao Fujiyama, Aldwin Anterola, Setsuyuki Aoki, Neil Ashton, W. Brad Barbazuk, Elizabeth Barker, Jeffrey L. Bennetzen, Robert Blankenship, Sung Hyun Cho, Susan K. Dutcher, Mark Estelle, Jeffrey A. Fawcett, Heidrun Gundlach, Kousuke Hanada, Alexander Heyl, Karen A. Hicks, Jon Hughes, Martin Lohr, Klaus Mayer, Alexander Melkozernov, Takashi Murata, David R. Nelson, Birgit Pils, Michael Prigge, Bernd Reiss, Tanya Renner, Stephane Rombauts, Paul J. Rushton, Anton Sanderfoot, Gabriele Schween, Shin-Han Shiu, Kurt Stueber, Frederica L. Theodoulou, Hank Tu, Yves Van de Peer, Paul J. Verrier, Elizabeth Waters, Andrew Wood, Lixing Yang, David Cove, Andrew C. Cuming, Mitsuyasu Hasebe, Susan Lucas, Brent D. Mishler, Ralf Reski, Igor V. Grigoriev, Ralph S. Quatrano, Jeffrey L. Boore
    SCIENCE 319 5859 64 - 69 2008年01月 [査読有り][通常論文]
    We report the draft genome sequence of the model moss Physcomitrella patens and compare its features with those of flowering plants, from which it is separated by more than 400 million years, and unicellular aquatic algae. This comparison reveals genomic changes concomitant with the evolutionary movement to land, including a general increase in gene family complexity; loss of genes associated with aquatic environments ( e. g., flagellar arms); acquisition of genes for tolerating terrestrial stresses ( e. g., variation in temperature and water availability); and the development of the auxin and abscisic acid signaling pathways for coordinating multicellular growth and dehydration response. The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant's unique facility for reverse genetics.
  • Masaki Odahara, Takayuki Inouye, Tomomichi Fujita, Mitsuyasu Hasebe, Yasuhiko Sekine
    GENES & GENETIC SYSTEMS 82 1 43 - 51 2007年02月 [査読有り][通常論文]
    Homologous recombination is a universal process that contributes to genetic diversity and genomic integrity. Bacterial-type RecA generally exists in all bacteria and plays a crucial role in homologous recombination. Although RecA homologues also exist in plant mitochondria, there have been few reports about the in vivo functions of these homologues. We identified a recA gene orthologue (named PprecA1) in a cDNA library of the moss, Physcomitrella patens. N-terminal fusion of the putative organellar targeting sequence of PpRecA1 to GFP caused a targeting of PpRecA1 to mitochondria. PprecA1 partially complemented the effects of a DNA damaging agent in an Escherichia coli recA deficient strain. Additionally, the expression of PprecA1 was induced by treating the plants with DNA damaging agents. Disruption of PprecA1 by targeted replacement resulted lower rate of the recovery of the mitochondrial DNA from methyl methan sulfonate damage. This is the first report about the characteristics of a null mutant of bacterial-type recA gene in plant. The data suggest that PprecA1 participates in the repair of mitochondrial DNA in P. patens.
  • Yoshihisa Oda, Toshio Sano, Tomomichi Fujita, Yuji Hiwatashi, Yoshikatsu Sato, Natsumaro Kutsuna, Aiko Hirata, Mitsuyasu Hasebe, Seiichiro Hasezawa
    PLANT AND CELL PHYSIOLOGY 48 S24 - S24 2007年 [査読有り][通常論文]
  • Tomomichi Fujita, Kaoru Hashimoto, Yuji Hiwatashi, Yoshikatsu Sato, Takashi Murata, Mitsuyasu Hasebe
    PLANT AND CELL PHYSIOLOGY 48 S50 - S50 2007年 [査読有り][通常論文]
  • Yuji Hiwatashi, Tomomichi Fujita, Takashi Murata, Mitsuyasu Hasebe
    PLANT AND CELL PHYSIOLOGY 48 S88 - S88 2007年 [査読有り][通常論文]
  • Mitsuyasu Hasebe, Tomoaki Nishiyama, Takako Tanahashi, Naoki Aono, Tsuyoshi Aoyama, Chaoyang Cheng, Tomomichi Fujita, Kaoru Hashimoto, Tadayoshi Hirai, Yuji Hiwatashi, Masaki Ishikawa, Mineko Iwata, Minoru Kubo, Tetsuya Kurata, Koij Mikami, Saori Miyazaki, Shin-Ichi Morinaga, Takashi Murata, Mari Obara, Yasuko Oguri, Naoko Onodera, Yoshikatsu Sato, Naomi Sumikawa, Naoki Shinohara, Sachiko Wakaduki, Nagisa Sugimoto
    PLANT AND CELL PHYSIOLOGY 48 S50 - S50 2007年 [査読有り][通常論文]
  • Terakura S, Kitakura S, Ishikawa M, Ueno Y, Fujita T, Machida C, Wabiko H, Machida Y
    Plant & cell physiology 47 5 664 - 672 2006年05月 [査読有り][通常論文]
    The 6b gene in the T-DNA region of the Ti plasmids of Agrobacterium tumefaciens and A. vitis is able to generate shooty calli in phytohormone-free culture of leaf sections of tobacco transformed with 6b. In the present study, we report characteristic morphological abnormalities of the leaves of transgenic tobacco and Arabidopsis that express 6b from pTiAKE10 (AK-6b), and altered expression of genes related to cell division and meristem formation in the transgenic plants. Cotyledons and leaves of both transgenic tobacco and Arabidopsis exhibited various abnormalities including upward curling of leaf blades, and transgenic tobacco leaves produced leaf-like outgrowths from the abaxial side. Transcripts of some class 1 KNOX homeobox genes, which are thought to be related to meristem functions, and cell cycle regulating genes were ectopically accumulated in mature leaves. M phase-specific genes were also ectopically expressed at the abaxial sides of mature leaves. These results suggest that the AK-6b gene stimulates the cellular potential for division and meristematic functions preferentially in the abaxial side of leaves and that the leaf phenotypes generated by AK-6b are at least in part due to such biased cell division during polar development of leaves. The results of the present experiments with a fusion gene between the AK-6b gene and the glucocorticoid receptor gene showed that nuclear import of the AK-6b protein was essential for upward curling of leaves and hormone-free callus formation, suggesting a role for AK-6b in nuclear events.
  • Machida M, Takechi K, Sato H, Chung SJ, Kuroiwa H, Takio S, Seki M, Shinozaki K, Fujita T, Hasebe M, Takano H
    Proceedings of the National Academy of Sciences of the United States of America 103 17 6753 - 6758 2006年04月 [査読有り][通常論文]
    The general consensus is that a cyanobacterium phagocytosed by a host cell evolved into the plastids of red and green algae, land plants, and glaucophytes. In contrast to the plastids of glaucophytes, which retain a cyanobacterial-type peptidoglycan layer, no wall-like structures have been detected in plastids from other sources. Although the genome of Arabidopsis thaliana contains five genes that are essential for peptidoglycan synthesis, MurE, MurG, two genes for D-Ala-D-Ala ligase (Ddl), and the gene for translocase I (MraY), their functions have not been determined. We report that the moss Physcomitrella patens has nine homologous genes related to peptidoglycan biosynthesis: MurA, B, C, D, E, and F, Ddl, genes for the penicillin-binding protein Pbp, and dd-carboxypeptidase (Dac). Corroborating a computer prediction, analysis of the GFP fusion proteins with the N terminus of PpMurE or of PpPbp suggests that these proteins are located in the chloroplasts. Gene disruption of the PpMurE gene in P. patens resulted in the appearance of macrochloroplasts both in protonema and in leaf cells. Moreover, gene knockout of the P. patens Pbp gene showed inhibition of chloroplast division in this moss; however, no Pbp gene was found in A. thaliana.
  • 藤田 知道
    植物の生長調節 41 2 156 - 162 一般社団法人植物化学調節学会 2006年
  • A Hayashida, K Takechi, M Sugiyama, M Kubo, RD Itoh, S Takio, T Fujita, Y Hiwatashi, M Hasebe, H Takano
    PLANT BIOLOGY 7 3 300 - 306 2005年05月 [査読有り][通常論文]
    Eleven mutant lines exhibiting decreased numbers of chloroplasts per cell were isolated from 8800 tagged mutant lines of Physcomitrella patens by microscopic observations. Chloronema subapical cells in wild-type plants had a mean of 48 chloroplasts, whereas chloroplast numbers in subapical cells in mutant lines 215 and 222 decreased to 75% of that in the wild type. Seven mutant lines - 473, 122, 221, 129, 492, 207, and 138 had about half as many chloroplasts as the wild type. Mutant line 11 had a few remarkably enlarged chloroplasts, and mutant line 347 had chloroplasts of various sizes. Whereas the cell volume was the same as in the wild type in mutant lines 222, 473, 221, 129, 492, and 207, the cell volume of the other mutants increased. The chloroplast number of leaf cells was the same as that of chloronema cells in each mutant line when gametophores could be formed. Treatment with ampicillin decreased the number of chloroplasts in all mutant lines. Southern hybridization using DNA in tags as probes showed that only one insertion occurred in mutant lines 473 and 221. To determine whether the tagged DNA inserted into the known genes for plastid division, we isolated the PpMinD1, PpMinD2, and PPMinE1 genes. Genomic polymerase chain reaction analysis showed that the PpFtsZ and PpMinD/E genes were not disrupted by the insertion of the tags in mutant lines 11 and 347, respectively.
  • Hiroyuki Eda, Yasuyuki Ishii, Maya Obayashi, Shizuko Harada, Sayuri Ito, Tomomichi Fujita, Masato Ikeda, Shuichi Kusano, Ryo Kitamura, Chieko Suzuki, Takahiko Hara, Motoo Watanabe, Hiroshi Satoh, Keisuke Sugihara, Kazuo Yanagi
    Virus Research 109 1 87 - 94 2005年04月
  • Gene tagging, gene- and enhancer-trap systems, and full-length cDNA overexpression in Physcomitrella patens. In New Frontiers in Bryology:Physiology, Molecular Biology & Functional Genomics (eds. by Wood, AJ., Oliver, MJ. and Cove, DJ.), Kluwer Academ・・・
    Fujita, T., Nishiyama, T., Hiwatashi, Y., and Hasebe, M.
    In New Frontiers in Bryology:Physiology, Molecular Biology & Functional Genomics (eds. by Wood, AJ., Oliver, MJ. and Cove, DJ.), Kluwer Academ・・・ 2004年 [査読無し][通常論文]
    Gene tagging, gene- and enhancer-trap systems, and full-length cDNA overexpression in Physcomitrella patens. In New Frontiers in Bryology:Physiology, Molecular Biology & Functional Genomics (eds. by Wood, AJ., Oliver, MJ. and Cove, DJ.), Kluwer Academic Publishers, Netherlands, pp. 111-132.
  • T Nishiyama, T Fujita, T Shin-I, M Seki, H Nishide, Uchiyama, I, A Kamiya, P Carninci, Y Hayashizaki, K Shinozaki, Y Kohara, M Hasebe
    The mosses and flowering plants diverged >400 million years ago. The mosses have haploid-dominant life cycles, whereas the flowering plants are diploid-dominant. The common ancestors of land plants have been inferred to be haploid-dominant, suggesting that genes used in the diploid body of flowering plants were recruited from the genes used in the haploid body of the ancestors during the evolution of land plants. To assess this evolutionary hypothesis, we constructed an EST library of the moss Physcomitrella patens, and compared the moss transcriptome to the genome of Arabidopsis thaliana. We constructed full-length enriched cDNA libraries from auxin-treated, cytokinin-treated, and untreated gametophytes of P. patens, and sequenced both ends of >40,000 clones. These data, together with the mRNA sequences in the public databases, were assembled into 15,883 putative transcripts. Sequence comparisons of A. thaliana and P. patens showed that at least 66% of the A. thaliana genes had homologues in A patens. Comparison of the A patens putative transcripts with all known proteins, revealed 9,907 putative transcripts with high levels of similarity to vascular plant genes, and 850 putative transcripts with high levels of similarity to other organisms. The haploid transcriptome of A patens appears to be quite similar to the A. thaliana genome, supporting the evolutionary hypothesis. Our study also revealed that a number of genes are moss specific and were lost in the flowering plant lineage.
  • Tomomichi Fujita, Albino Maggio, Mario Garcı́a-Rı́os, Cynthia Stauffacher, Ray A. Bressan, Laszlo N. Csonka
    Journal of Biological Chemistry 278 16 14203 - 14210 2003年04月18日
  • Maggio A, Miyazaki S, Veronese P, Fujita T, Ibeas JI, Damsz B, Narasimhan ML, Hasegawa PM, Joly RJ, Bressan RA
    PLANT JOURNAL 31 6 699 - 712 2002年09月 [査読有り][通常論文]
  • S Kitakura, T Fujita, Y Ueno, S Terakura, H Wabiko, Y Machida
    PLANT CELL 14 2 451 - 463 2002年02月 
    The 6b gene in the T-DNA from Agrobacterium has oncogenic activity in plant cells, inducing tumor formation, the phytohormone-independent division of cells, and alterations in leaf morphology. The product of the 6b gene appears to promote some aspects of the proliferation of plant cells, but the molecullar mechanism of its action remains unknown. We report here that the 6b protein associates with a nuclear protein in tobacco that we have designated NtSI1 (for Nicotiana tabacum 6b-interacting protein 1). NtSIP1 appears to be a transcription factor because its predicted amino acid sequence includes two regions that resemble a nuclear localization signal and a putative DNA binding motif, which is similar in terms of amino acid sequence to the triple helix motif of rice transcription factor GT-2. Expression in tobacco cells of a fusion protein composed of the DNA binding domain of the yeast GAL4 protein and the 6b protein activated the transcription of a reporter gene that was under the control of a chimeric promoter that included the GAL4 upstream activating sequence and the 35S minimal promoter of Cauliflower mosaic virus. Furthermore, nuclear localization of green fluorescent protein-fused 6b protein was enhanced by NtSIP1. A cluster of acidic residues in the 6b protein appeared to be essential for nuclear localization and for transactivation as well as for the hormone-independent growth of tobacco cells. Thus, it seems possible that the 6b protein might function in the proliferation of plant cells, at least in part, through an association with NtSIP1.
  • Y Hiwatashi, T Nishiyama, T Fujita, M Hasebe
    PLANT JOURNAL 28 1 105 - 116 2001年10月 [査読有り][通常論文]
    Because of its simple body plan and ease of gene knockout and allele replacement, the moss Physcomitrella patens is often used as a model system for studies in plant physiology and developmental biology. Gene-trap and enhancer-trap systems are useful techniques for cloning genes and enhancers that function in specific tissues or cells. Additionally, these systems are convenient for obtaining molecular markers specific for certain developmental processes. Elements for gene-trap and enhancer-trap systems were constructed using the uidA reporter gene with either a splice acceptor or a minimal promoter. Through a high rate of transformation conferred by a method utilizing homologous recombination, 235 gene-trap and 1073 enhancer-trap lines were obtained from 5637 and 3726 transgenic lines, respectively. The expression patterns of these trap lines in the moss gametophyte varied. The candidate gene trapped in a gene-trap line YH209, which shows rhizoid-specific expression, was obtained by 5' and 3' RACE. This gene was named PpGLU, and forms a clade with plant acidic alpha -glucosidase genes. Thus, these gene-trap and enhancer-trap systems should prove useful to identify tissue- and cell-specific genes in Physcomitrella.
  • 岩井 美穂, 斎藤 茂樹, CHON Nguyen Minh, 桑原 明日香, 原田 英美子, 朝比奈 雅志, 小田 篤, DA SILVA Jaime A. T., 野村 崇人, 関 原明, 藤田 知道
    植物の生長調節 36 2 222 - 228 一般社団法人植物化学調節学会 2001年
  • Tomomichi Fujita, Masato Ikeda, Shuichi Kusano, Makoto Yamazaki, Sayuri Ito, Maya Obayashi, Kazuo Yanagi
    Intervirology 44 5 271 - 282 2001年
  • R.A. Salzman, T. Fujita, K. Zhu-Salzman, P.M. Hasegawa & R.A. Bressan
    Plant Mol Biol Rep 17 1 11 - 17 1999年
  • M. Garcia-Rios, T. Fujita, P. C. LaRosa, R. D. Locy, J. M. Clithero, R. A. Bressan, L. N. Csonka
    Proceedings of the National Academy of Sciences 94 15 8249 - 8254 1997年07月22日
  • Cloning of tomPRO1 and tomPRO2 from Lycopersicon esculentum L.: coexistence of polycistronic and monocistronic genes which encode the enzymes catalyzing the first two steps of proline biosynthesis.
    A. Maggio, M. Garcia-Rios, T. Fujita, R.A. Bressan, R.J. Joly, P.M. Hasegawa, L.N. Csonka
    112 862 - 862 1996年
  • Seishiro Aoki, Akiyoshi Kawaoka, Masami Sekine, Takanari Ichikawa, Tomomichi Fujita, Atsuhiko Shinmyo, Kunihiko Syono
    MGG Molecular & General Genetics 243 6 706 - 710 1994年11月 
    A region homologous to the TL-DNA of Agrobacterium rhizogenes was previously detected in the genome of untransformed Nicotiana glauca and designated cellular T-DNA (cT-DNA). Subsequently, part of this region was sequenced and two genes, which corresponded to rolB and rolC and were named Ng rolB and Ng rolC, were found. We have now sequenced a region of the cT-DNA other than the region that includes Ng rolB and C and we have found two other open reading frames (ORFs), NgORF13 and NgORF14. These ORFs correspond to ORFs 13 and 14 of the TL-DNA of A. rhizogenes and exhibit a high degree of homology to these ORFs, without having a nonsense codon. We have not found any sequence homologous to rolD (ORF15). The two genes, NgORF13 and 14, as well as the Ng rolB and C genes, are expressed in genetic tumors of hybrids between N. glauca and N. langsdorffii but not in leaf tissues of the hybrid. © 1994 Springer-Verlag.
  • Tomomichi Fujita, Hiroshi Kouchi, Takanari Ichikawa, Kunihiko Syõno
    Plant J. 5 5 645 - 654 1994年05月 [査読有り]
  • 藤田 知道
    植物組織培養 11 3 171 - 177 Japanese Society for Plant Cell and Molecular Biology 1994年
  • FUJITA Tomomichi, KOUCHI Hiroshi, ICHIKAWA Takanari, SYONO Kunihiko
    Plant and cell physiology 34 1 137 - 142 Japanese Society of Plant Physiologists 1993年01月 [査読有り]
    We have isolated a cDNA clone, designated GTI, by screening a tobacco genetic tumor cDNA library with a tumor-specific "subtracted" cDNA probe. The cDNA contained the entire coding sequence for a 94-amino-acid polypeptide that exhibited significant homology to members of the proteinase inhibitor I family from tomato and potato. The predicted protein has a pre-sequence of 22 amino acids but lacks a pro-sequence, unlike genes for proteinase inhibitor I isolated to date. Furthermore, the protein encoded by GTI cDNA has a novel reactive site, having glutamine as the P_1 reactive residue. These results suggest that the GTI protein is a novel member of the proteinase inhibitor I family. The mRNA for GTI accumulated at a high level but only transiently after the wounding of tobacco plants. Thus, it appears that the GTI protein has a function that is related to the protection of tissues against damage due to wounding.
  • Fujita Tomomichi, Ichikawa Takanari, Syono Kunihiko
    Plant and cell physiology 32 2 169 - 177 Japanese Society of Plant Physiologists 1991年03月 [査読有り]
    When normal shoots which had been regenerated from tobacco genetic tumor cultured in the dark were cut, tumorous tissues were again induced on the cut segments. Morphological examination of the segments, 5 days and 15days after cutting, in a comparison with normally regenerated shoots, revealed active cell division in vascular-bundle tissue and formation of primordial teratoma-like structures in the induced tumorous tissues. Endogeneous IAA remained at a constant, low level throughout the tumorigenetic process. Proteins from each step of tumorigenesis were separated by two-dimensional electrophoresis on mini-gels and visualized by silver staining. Twenty-seven polypeptides showed qualitative or quantitative changes during the tumorigenetic process. These results are discussed in relation to the development of tobacco genetic tumor.


  • コケ分子生物学を用いたSDGsへの取り組み
    アグリバイオ 7 (3) 24-28、北隆館 2023年03月
  • 植物のサイクリン依存性キナーゼCDKAの光応答における新たな機能
    井上夏実, 藤田知道 
    バイオサイエンスとインダストリー 80巻 pp.414-416、バイオインダストリー協会 2022年09月
  • 環境ストレスにより新生するコケ植物の幹細胞
    神野智世, 藤田知道 
    アグリバイオ 6 (6) 82-86、北隆館 2022年06月
  • コケ植物の幹細胞新生にみるアブシジン酸を用いたしなやかな生存戦略
    神野智世, 藤田知道 
    細胞 54 (6) 61-64、ニューサイエンス社 2022年05月
  • Tracking intercellular movement of fluorescent proteins in bryophytes.
    Takumi Tomoi, Yoan Coudert, Tomomichi Fujita (担当:分担執筆範囲:Plasmodesmata, Methods and Protocols, In Methods in Molecular Biology (MIMB), volume 2457, 321-332; https://doi.org/10.1007/978-1-0716-2132-5_22)
    Humana, New York 2022年03月 (ISBN: 9781071621318)
  • Arthur Muller, Tomomichi Fujita, Yoan Coudert (担当:分担執筆範囲:Plasmodesmata, Methods and Protocols, In Methods in Molecular Biology (MIMB), volume 2457, 177-187; https://doi.org/10.1007/978-1-0716-2132-5_11)
    Humana, New York 2022年03月 (ISBN: 9781071621318) 177-187 
    Callose detection and quantification at plasmodesmata in bryophytes.
  • An experimental system for examining phototropic response of gametophytic shoots in the moss Physcomitrella patens
    Liang Bao, Kotaro Yamamoto, Tomomichi Fujita (担当:分担執筆範囲:Phototropism, In Methods in Molecular Biology, 1924, 45-51. doi: 10.1007/978-1-4939-9015-3_5)
    Humana Press, New York, NY 2019年 (ISBN: 9781493990153)
  • 植物栽培における重力環境制御の基礎
    唐原一郎, 玉置大介, 久米篤, 蒲池浩之, 半場祐子, 藤田知道 
    アグリバイオ 10(1), 1172-1175、北隆館 2017年10月
  • 植物生理学概論 改訂版
    櫻井英博, 柴岡弘郎, 高橋陽介, 小関良宏, 藤田知道 (担当:分担執筆)
    培風館 2017年09月
  • 植物ゲノム科学辞典
    藤田知道, 駒嶺穆, 町田泰則, 藤村達人, 田畑哲之, 三位正洋, 斉藤和季 (担当:その他)
    朝倉書店 2009年
  • 植物の百科事典「細胞極性、不等分裂」
    藤田知道, 石井龍一, 岩槻邦男, 長谷部光泰, 矢澤進, 矢原徹一, 和田正三 (担当:その他)
    朝倉書店 2009年
  • 植物細胞工学シリーズ23、植物の進化 「発生遺伝子の進化」
    長谷部光泰, 藤田知道, 倉田哲也, 佐藤良勝, 久保稔, 村田隆, 青山剛士, 三上浩司, 石川雅樹, 日渡祐二, 青野直樹, 篠原直貴, 西山智明, 棚橋貴子 (担当:共著)
    秀潤社 2007年
  • 植物ホルモンの分子細胞生物学「セン類、タイ類の植物ホルモン」
    藤田知道, 小柴共一, 神谷勇治, 勝見允行 
    講談社サイエンティフィク 2006年
  • ヒメツリガネゴケを用いた植物幹細胞の不等分裂過程の解析−細胞極性形成から娘細胞の運命決定まで−
    植物化学調節学会 2006年
  • Gene tagging, gene- and enhancer-trap systems, and full-length cDNA overexpression in Physcomitrella patens
    Fujita, T, Nishiyama, T, Hiwatashi, Y, Hasebe, M (担当:共著)
    Kluwer Academic Publishers 2004年
  • 植物細胞工学シリーズ20、新版植物ホルモンのシグナル伝達 「植物ホルモンの起源を探る—シダ植物,コケ植物,緑色藻類における植物ホルモンの役割から—」
    藤田 知道 
    秀潤社 2004年
  • 学術月報「3度のポスドク体験から」
    藤田 知道 
    日本学術振興会 2000年
  • タバコ遺伝的腫瘍の腫瘍形成に関わる遺伝子の検索
    藤田 知道 
    植物組織培養 1994年
  • DNA複製機構の解明に向けてーDNA腫瘍ウイルスの手助け
    藤田 知道 
    生物工学会誌 1994年
  • Habituation as a tumorous state that is interchangeable with a normal state in plant cells
    Syono, K, Fujita, T (担当:共著)
    International Review of Cytology (eds. by Jeon, K. W. and Jarvik J.), 152, pp.265-299, Academic Press, 1994年



  • 2018年 日本植物学会 Journal of Plant Research Best paper論文賞
    受賞者: 藤田 知道
  • 2014年 北海道大学 北海道大学教育総長賞
    受賞者: 藤田 知道
  • 2014年 日本植物学会 Journal of Plant Research Best paper論文賞
    受賞者: 藤田 知道
  • 2014年 日本学術振興会 特別研究員等審査会専門委員及び国際事業委員会書面審査委員表彰者
    受賞者: 藤田 知道


  • スペース・モス創出-テラフォーミングを先導するコケ植物力利用技術開発の挑戦的研究
    研究期間 : 2023年
  • 宇宙におけるコケ植物の環境応答と宇宙利用(スペース・モス)
    研究期間 : 2015年12月 
    代表者 : https://humans-in-space.jaxa.jp/kibouser/subject/life/70682.html
  • ヒメツリガネゴケを用いた植物細胞極性、不等分裂、分化全能性の仕組みの解明
  • Molecular characterization of cell polarity, asymmetric cell division and totipotency in plants
  • 植物の原形質連絡の透過度を制御する謎の分子機構解明に挑む
  • 植物発生における軸と情報の分子基盤
  • 植物メリステムと器官の発生を支える情報統御系
  • 植物の生命力を支える多能性幹細胞の基盤原理



  • 2023年10月 - 現在   日本学術会議連携会員
  • 2023年09月 - 現在   日本宇宙生物科学会   広報委員長
  • 2023年09月 - 現在   日本宇宙生物科学会   代議員・理事
  • 2022年03月 - 現在   日本植物生理学会   広報委員
  • 2022年01月 - 現在   日本植物生理学会   代議員
  • 2021年 - 現在   Frontiers in Plant Science,   Review Editor in Plant Physiology
  • 2021年 - 現在   Frontiers in Plant Science,   Associate Editor for Plant Development and EvoDevo
  • 2020年01月 - 現在   国際生物学オリンピック日本委員会   委員
  • 2020年 - 現在   Plants, Editorial board
  • 2019年 - 現在   新エネルギー・産業技術総合開発機構(NEDO)分野横断的公募事業に係る事前書面審査審査員
  • 2016年 - 現在   iMOSS-international molecular moss science society, Extended board member
  • 2009年 - 現在   Faculty of 1000 (F1000), faculty member
  • 2022年09月 - 2023年09月   日本植物学会第77回大会   大会副会長
  • 2021年 - 2023年03月   日本植物学会   理事
  • 2020年 - 2022年06月   北海道植物学会   会長
  • 2020年 - 2022年06月   日本植物学会   代議員
  • 2020年01月 - 2021年12月   日本植物生理学会   代議員
  • 2019年 - 2021年   Plant Molecular Biology, Lead guest editor
  • 2015年 - 2021年   Frontiers in Plant Science,   Review Editor for Plant Development and EvoDevo
  • 2017年 - 2020年   Journal of Plant Research 編集委員
  • 2016年 - 2018年   日本植物学会賞選考委員
  • 2016年 - 2018年   日本植物学会代議員
  • 2014年 - 2016年   日本植物学会代議員
  • 2014年 - 2015年   Plant and Cell Physiology 編集委員
  • 2014年 - 2015年   日本植物生理学会代議員
  • 2013年 - 2014年   北海道植物学会会長
  • 2012年 - 2014年   日本植物学会代議員
  • 2010年 - 2013年   Plant and Cell Physiology 編集委員
  • 2011年 - 2012年   日本植物学会評議員
  • 2010年 - 2012年   日本植物学会広報委員会ホームページコンテンツ作成ワーキンググループ委員
  • 2010年 - 2011年   日本植物生理学会評議員
  • 2008年 - 2009年   日本植物生理学会評議員


  • YAC種子島スペースキャンプ2023夏
    期間 : 2023年07月30日 - 2023年08月01日
    役割 : 講師
    主催者・発行元 : YAC 日本宇宙少年団
    イベント・番組・新聞雑誌名 : [https://www.yac-j.com/feature/tanegashima-summer2023/]
  • 千葉県船橋市立西海神小学校「総合:キャリア教育」6年生:ゲストティーチャー
    期間 : 2023年02月
    役割 : 講師
  • 北海道大学オープンキャンパス(高校生限定プログラム)(2010〜2019年, 2022〜2023年)
    期間 : 2010年 - 2023年
    役割 : 講師
  • ほっとニュース北海道(報道)「植物の成長を調整するたんぱく質発見」
    期間 : 2022年12月14日
    役割 : 取材協力
    主催者・発行元 : NHK札幌
    イベント・番組・新聞雑誌名 : ほっとニュース北海道(2023年1月30日放映)
  • 企画展および企画展記念講演会 こけティッシュ・プレミアムトーク
    期間 : 2022年01月09日
    役割 : パネリスト
    主催者・発行元 : 茨城県自然博物館
    イベント・番組・新聞雑誌名 : 企画展「こけティッシュ 苔ニューワールド!―地球を包むミクロの森―」
  • 千葉県船橋市立西海神小学校「総合:キャリア教育」6年生:ゲストティーチャー
    期間 : 2021年02月
    役割 : 講師
  • 高校生向け 学問新サイト「みらいぶっく・学問・大学なび」掲載
    期間 : 2020年05月
    役割 : 取材協力
    主催者・発行元 : 河合塾
  • NASA Media Teleconference(出演)
    期間 : 2019年07月10日
    役割 : 出演
    主催者・発行元 : National Aeronautics and Space Administration (NASA)
  • ほっとニュース北海道 :ブーム到来中!宇宙に飛び立つ「コケ」
    期間 : 2016年08月01日
    役割 : 出演
    主催者・発行元 : NHK札幌
    イベント・番組・新聞雑誌名 : ほっとニュース北海道:ほっと中継
  • 文化放送いとうせいこう GREEN FESTA,「スペース・モス」の宇宙実験がJAXAで採用!
    期間 : 2016年02月16日
    役割 : 取材協力
    イベント・番組・新聞雑誌名 : 文化放送(2016年2月16日ON AIR)
  • 札幌藻岩高校SPP(サイエンス・パートナーシップ・プロジェクト)
    期間 : 2009年09月25日
    役割 : 講師
  • 札幌開成高校(出前授業)
    期間 : 2006年11月
    役割 : 講師
  • 広幡小学校 出前授業「科学教室:生き物の遺伝物質をとってみよう」
    期間 : 2004年02月
    役割 : 講師
    主催者・発行元 : 岡崎市立広幡小学校・岡崎ロータリークラブ

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