研究者データベース

佐野 英彦(サノ ヒデヒコ)
歯学研究院 口腔医学部門 口腔健康科学分野
教授

基本情報

所属

  • 歯学研究院 口腔医学部門 口腔健康科学分野

職名

  • 教授

学位

  • 博士(歯学)

J-Global ID

研究キーワード

  • 象牙質接着   ナノテクノロジー   齲蝕象牙質   接着界面   象牙質の特性   Caries dentin   Adhesive interface   Characteristics of dentin   

研究分野

  • ライフサイエンス / 保存治療系歯学

学歴

  •         - 1987年   東京医科歯科大学   歯学研究科   歯学
  •         - 1987年   東京医科歯科大学
  •         - 1983年   東京医科歯科大学   歯学部   歯学
  •         - 1983年   東京医科歯科大学

所属学協会

  • 日本歯科保存学会   日本歯科理工学会   接着歯学   国際歯科学会(International Association for Dental Research)   口腔病学会   

研究活動情報

論文

  • Hidehiko Sano, Abu Faem Mohammad Almas Chowdhury, Pipop Saikaew, Mariko Matsumoto, Shuhei Hoshika, Monica Yamauti
    The Japanese dental science review 56 1 24 - 31 2020年12月 [査読有り][通常論文]
     
    Microtensile bond strength (μTBS) test was introduced in 1994. Since then, it has been utilized profoundly across many bond strength testing laboratories, making it currently one of the most standard and versatile bond strength test. Although it is a static and strength-based method, together with the morphological and spectroscopic investigations, it has been contributing immensely in the advancement of dentin adhesive systems. μTBS test has a greater discriminative capability than the traditional macro-shear bond test. During the early stage of its development, the authors predicted that this testing method would enable evaluation of the adhesive performances of resins to excavated carious or sclerotic dentin and the regional bond strengths of various portions of the cavity. In addition, they also stated the possibility of comparing the long-term stability of resin adhesion at various portions of the cavity walls on teeth extracted at various times after insertion of bonded restorations. In this review, we discussed the historical background, inception and the application of the μTBS test and proposed directions for further improvement of this testing method.
  • Pipop Saikaew, Mariko Matsumoto, Vanthana Sattabanasuk, Choltacha Harnirattisai, Ricardo M Carvalho, Hidehiko Sano
    European journal of oral sciences 2020年05月12日 [査読有り][通常論文]
     
    Using transmission electron microscopy (TEM), this study investigated the characteristics of smear layers after preparation of dentin surfaces using different methods. In addition, this study analyzed, using scanning electron microscopy (SEM), the interactions of smear layers with the various acidic agents used subsequently to treat the dentin surface. Mid-coronal dentin surfaces were prepared according to one of five instrumentation methods: fracture; grinding with carbide bur; grinding with regular-grit diamond bur; grinding with superfine-grit diamond bur; or grinding with 600-grit silicon carbide (SiC) abrasive paper. Then, each prepared dentin surface was further divided into control (no further treatment) or treatment with one of the following agents: phosphoric acid solution (PAS); phosphoric acid gel (PAG); Clearfil SE Bond 2 Primer (SE2); or Scotchbond Universal Adhesive (SBU). Longitudinal ultra-thin sections were made for TEM observations. Dentin discs, representing each surface preparation and treatment method described above, were created, and the morphology was examined by SEM. The TEM results revealed differences in thickness and denseness of the smear layer, according to the different methods of instrumentation used to prepare the dentin surface. The two forms of phosphoric acid completely removed the smear layer in all groups. Agglomerated silica particles were observed on the top of the collagen layer after etching with PAG. For all dentin surfaces, except for that prepared with SiC, SE2 demonstrated better etching efficacy on different smear layers, whereas SBU provided only superficial interaction.
  • Size- and morphology- controlled preparation of surface-modified water-dispersible fullerene nanoparticles for bioapplications
    H. Oguma, E. Seitoku, M. Mutoh, S. Yoshizawa, K. Nakanishi, Y. Bando, Y. Era, T. Kiba, P. Saikaew, M. Tamai, T. Akasaka, M. Nakamura, T. Kusaka, Y. Yoshida, Y. Sato, H. Sano, S. Abe, A. Valanezhad, I. Watanabe, S. Inoue, T. Takada
    J. Nanoscience and Nanotechnology 20 1 2668 - 2674 2020年01月 [査読有り][通常論文]
  • Abu Faem Mohammad Almas Chowdhury, Rafiqul Islam, Arefin Alam, Mariko Matsumoto, Monica Yamauti, Ricardo Marins Carvalho, Hidehiko Sano
    International journal of molecular sciences 20 21 2019年10月29日 [査読有り][通常論文]
     
    The removal or modification of smear layers that cover the dentin is critical to allow the penetration of adhesive molecules and to ensure a strong bond between resin and dentin. Aiming to establish a model for clinically-relevant dentin-bond testing, we evaluated the effects of smear layers created by abrasives having similar coarseness (180-grit SiC paper; fine-grit diamond bur) and application modes (single application; double application) on the microtensile bond strengths (µTBS) of two currently available universal adhesives (G-Premio Bond; Scotchbond Universal Adhesive) and a two-step self-etch adhesive (Clearfil Megabond 2). Sixty extracted human third molars were used for the μTBS test. Data were analyzed by three-way ANOVA and Tukey's test (α = 0.05). Fracture modes were determined using stereomicroscopy. An additional 24 third molars were prepared for observation of the resin-dentin interface by TEM and adhesive-smear layer interaction by SEM. μTBS was significantly affected by the adhesives and their application modes (p < 0.001), implying that the double application of universal adhesives should be recommended to improve their performance. The effect of smear layers was not significant (p > 0.05), indicating that 180-grit SiC papers could be used to prepare dentin as a substitute for fine-grit diamond burs for dentin-bond testing in laboratory settings.
  • Antibacterial effect of a fluoride-containing ZnO/CuO nanocomposite
    Yasuhiro Matsuda, Katsushi Okuyama, Hiroko Yamamoto, Mari Fujita, Shigeaki Abe, Takahiro Sato, Naoto Yamada, Masashi Koka, Hidehiko Sano, Mikako Hayashi, Sharanbir K. Sidhu, Takashi Saito
    Nuclear Instruments and Methods in Physics Research Section B 458 184 - 188 2019年07月 [査読有り][通常論文]
  • Fluorine distribution from fluoride-releasing luting materials into human
    Katsushi Okuyama, Yasuhiro Matsuda, Hiroko Yamamoto, Yukimichi Tamaki, Takashi Saito, Mikako Hayashi, Yasuhiro Yoshida, Hidehiko Sano, Takahiro Sato, Masashi Koka
    Nuclear Instruments and Methods in Physics Research Section B 456 16 - 20 2019年07月 [査読有り][通常論文]
  • Reproducibility in Testing Method with Stirring for in > Vitro Glass-ionomer Cement
    Shinichi KAKUDA, Shigeaki ABE, Pipop SAIKAEW, Almas AFM CHOWDHURY, Hidehiko SANO
    Nano Biomedicine accepted 2019年03月 [査読有り][通常論文]
  • Chowdhury AFMA, Saikaew P, Matsumoto M, Sano H, Carvalho RM
    Dental materials journal 38 3 361 - 367 2019年02月 [査読有り][通常論文]
  • Saikaew P, Fu J, Chowdhury AFMA, Carvalho RM, Sano H
    Clinical oral investigations 2018年10月 [査読有り][通常論文]
  • Saikaew P, Matsumoto M, Chowdhury A, Carvalho RM, Sano H
    Operative dentistry 43 5 549 - 558 2018年09月 [査読有り][通常論文]
  • Habiba U, Kuroshima T, Yanagawa-Matsuda A, Kitamura T, Chowdhury A, Jehung JP, Hossain E, Sano H, Kitagawa Y, Shindoh M, Higashino F
    Experimental cell research 369 2 218 - 225 2018年08月 [査読有り][通常論文]
  • Saikaew P, Senawongse P, Chowdhury AA, Sano H, Harnirattisai C
    Dental materials journal 2018年08月 [査読有り][通常論文]
  • Ting S, Chowdhury AFMA, Sun J, Kakuda S, Sidhu SK, Yoshida Y, Selimovic D, Sano H
    Dental materials journal 37 4 562 - 567 2018年07月 [査読有り][通常論文]
  • Hoshika S, Kameyama A, Suyama Y, De Munck J, Sano H, Van Meerbeek B
    The journal of adhesive dentistry 20 2 113 - 120 2018年 [査読有り][通常論文]
  • Mai Fukuyama, Chiharu Kawamoto, Pipop Saikaew, Yasuhiro Matsuda, Ricardo M. Carvalho, Denis Selimovic, Hidehiko Sano
    EUROPEAN JOURNAL OF ORAL SCIENCES 125 6 471 - 478 2017年12月 [査読有り][通常論文]
     
    This study evaluated the effect of topical fluoride application on enamel hardness after in-office bleaching. Twelve human incisors were cut along the long axis, resulting in 24 halves used in four treatment groups (n = 6 in each group): (i) untreated group (C); (ii) in-office bleaching material (B); (iii) treatment with surface reaction-type prereacted glass-ionomer varnish after in-office bleaching (B+PRG); and (iv) treatment with acidulated phosphate fluoride solution after bleaching (B+F). All specimens were subjected to pH-cycling for 4 wk. Knoop hardness was measured using a Cariotester. The decalcification of enamel was assessed quantitatively by measuring the integrated mineral loss (IML). Games-Howell analysis was used to assess statistical significance of between-group differences. The Knoop hardness decreased significantly after bleaching for all groups. In treatment groups B+PRG and B+F, the Knoop hardness returned to the original unbleached values after the first pH cycle and did not change afterwards. In treatment groups C and B there was a gradual decrease in the Knoop hardness until the fourth pH cycle. The integrated mineral loss, IML, was significantly higher in treatment group B+F after 2 wk than in the other treatment groups. After 4 wk, the IML in treatment group B was significantly higher than in treatment group B+PRG. The application of fluoride-containing materials after bleaching results in recuperation of hardness to levels similar to those of unbleached enamel.
  • Katsumata Aiichiro, Saikaew Pipop, Ting Shihchun, Katsumata Tamaki, Hoshika Tomohiro, Sano Hidehiko, Nishitani Yoshihiro
    Journal of Oral Tissue Engineering 15 1 18 - 24 日本再生歯科医学会 2017年09月 [査読無し][通常論文]
     
    実験的なユニバーサルアドヒーシブ(UA)であるK5D(トクヤマデンタル)の象牙質への微小引張り強さ(μTBS)と、その接着界面の微小形態を、スコッチボンドUA(SBU、3M ESPE)と比較した。平坦化したヒト臼歯の象牙質表面を600番のシリコンカーバイド紙で処理した8種類の試料を調製し、上記両UAの間で2種類の複合レジン(CR)、レジンコア(CO)および2種類のレジンセメント(CE)によるμTBSを比較した。また、COとCEについては光照射の有無で光の影響を比較した。その結果、μTBSは両UAによるCR接着体の間で有意な差を認めず、CO接着体との間では光照射の有無による有意な差は見られなかった。また、両UAによるCE接着体の間では、無照射SBUが、照射SBU、無照射/照射K5Dに比べ有意に低いμTBSを示した。以上より、無照射SBUはμTBSに負の影響を及ぼすが、K5Dは上記条件下で影響を受けないと考えられた。
  • Katsumata Aiichiro, Katsumata Tamaki, Hoshika Shuhei, Hoshika Tomohiro, Nishitani Tomiko, Yajima Takehiro, Sato Tadasu, Ichikawa Hiroyuki, Sano Hidehiko, Nishitani Yoshihiro
    Journal of Oral Tissue Engineering 15 1 11 - 17 日本再生歯科医学会 2017年09月 [査読無し][通常論文]
     
    コロイド状白金ナノ粒子(CPN)が象牙質接着強さに及ぼす影響を検討した。抜歯したヒト臼歯21本を対照群と2種類の実験群に分け、接着剤にはCLEARFILユニバーサルボンド(CUB)を用い、表面処理剤にはK ETCHANT syringe(ECH)とCPNを用いた。対照群はCUBのみで処理し、実験群はCUB+Ech(ECH群)またはCUB+CPN(CPN群)で処理して、樹脂複合材の形成後に37℃水中に24時間保管した後、接着面が1mm2の試料を作成して微小引張り強さ(μTBS)を試料調製の直後と6ヵ月後に測定した。その結果、24時間後の接着強さは対照群と実験群の間で差が見られなかったが、6ヵ月後に接着強さの有意な低下が見られなかったのはCPN群のみであった。以上より、CUBとCPNを併用すると長期の接着信頼性が得られると考えられた。
  • Jiale Fu, Pipop Saikaew, Shimpei Kawano, Ricardo M. Carvalho, Matthias Hannig, Hidehiko Sano, Denis Selimovic
    DENTAL MATERIALS 33 8 895 - 903 2017年08月 [査読有り][通常論文]
     
    Objectives. To evaluate the influence of different air-blowing durations on the micro-tensile bond strength (mu TBS) of five current one-step adhesive systems to dentin. Methods. One hundred and five caries-free human molars and five current one-step adhesive systems were used: ABU (All Bond Universal, Bisco, Inc.), CUB (CLEARFIL(TM) Universal Bond, Kuraray), GPB (G-Premio BOND, GC), OBA (OptiBond All-in-one, Kerr) and SBU (Scotch bond Universal, 3M ESPE). The adhesives were applied to 600 SiC paper-flat dentin surfaces according to each manufacturer's instructions and were air-dried with standard, oil-free air pressure of 0.25 MPa for either 0 s, 5 s,15 s or 30 s before light-curing. Bond strength to dentin was determined by using mu,TBS test after 24 h of water storage. The fracture pattern on the dentin surface was analyzed by SEM. The resin-dentin interface of untested specimens was visualized by panoramic SEM image. Data from ii,TBS were analyzed using two-way ANOVA (adhesive vs. air-blowing time), and Games-Howell (a = 0.05). Results. Two-way ANOVA revealed a significant effect of materials (p = 0.000) and air blowing time (p = 0.000) on bond strength to dentin. The interaction between factors was also significantly different (p = 0.000). Maximum bond strength for each system were recorded, OBA/15 s (76.34 +/- 19.15 MPa), SBU/15 s (75.18 +/- 12.83 MPa), CUB/15 s (68.23 +/- 16.36 MPa), GPB/30 s (55.82 +/- 12.99 MPa) and ABU/15 s (44.75 +/- 8.95 MPa). The maximum bond strength of OBA and SUB were significantly higher than that of GPB and ABU (p < 0.05). Significance. The bond strength of the current one-step adhesive systems is material dependent (p = 0.000), and was influenced by air-blowing duration (p = 0.000). For the current one-step adhesive systems, higher bond strengths could be achieved with prolonged air blowing duration between 15-30 s. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • Kumiko Yoshihara, Noriyuki Nagaoka, Yukinori Maruo, Hidehiko Sano, Yasuhiro Yoshida, Bart Van Meerbeek
    DENTAL MATERIALS 33 6 723 - 734 2017年06月 [査読有り][通常論文]
     
    Objective. Bioactive glasses and surface pre-reacted glass-ionomer (sPRG) filler possess cario-static properties owing to ion release. Many studies investigated potential cariostatic effects; few studies evaluated the surface stability and the structural changes their surfaces undergo in acidic conditions. Methods. The surface resistance against acid attack and the surface receptiveness for bacterial adhesion and biofilm formation of a sPRG-filled (Beautifil ll, Shofu) and conventional glass-filled (Herculite XRV Ultra, Kerr) resin-based composite (RBC), and a conventional glass-ionomer cement (GIC; Fuji IX GP Extra, GC) were examined. Specimens (n = 3) were immersed in distilled water or lactic acid (pH 4.0) for 3 days. Bacterial growth and biofilm formation were recorded using optical density and SEM. Results. Upon 3-day immersion in lactic acid, the surface of the sPRG-filled RBC revealed multiple holes, while virtually no change in surface integrity was observed for the conventional RBC and GIC. Bacterial growth measurements revealed that none of the materials inhibited Streptococcus mutans (p<0.05). Remarkably, cross-sectional SEM revealed that S. mutans had penetrated the etch pits induced by lactic acid in/around the sPRG filler. Ion-release measurements revealed that sPRG-filled RBC released boron and fluoride, while GIC only released fluoride. However, the concentration of ions released by both materials appeared not sufficient to inhibit bacterial growth. Moreover, the structural surface change and resultant increased surface roughness appeared to have promoted biofilm formation. Significance. While having bioactive potential through ion release, the stability of surface integrity of bioactive materials is a key-parameter to be assessed with regard to their cario-static potential. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • Estimation of Controlled Drug-release Behavior of Nano-porous Silica Micro Particles and Their Biocompatibility
    Eri SEITOKU, Yuko ERA, Ko NAKANISHI, Yosuke BANDO, Shinichi KAKUDA, Koichi NAKAMURA, Teruo KUSAKA, Shigeaki ABE, Mariko NAKAMURA, Yasutaka YAWAKA, Junichiro IIDA, Yasuhiro YOSHIDA, Hidehiko SANO
    Nano Biomedicine 9 2 112 - 116 2017年03月 [査読有り][通常論文]
  • K. Okuyama, Y. Tamaki, H. Yamamoto, K. Yagi, M. Hayashi, Y. Matsuda, T. Saito, K. Yasuda, K. Suzuki, H. Sano
    International Journal of PIXE 27 01n02 1 - 9 2017年 [査読有り][通常論文]
  • Atsunaka Sakurai, Tomoka Hasegawa, Ai Kudo, Zhao Shen, Tomoya Nagai, Miki Abe, Taiji Yoshida, Hiromi Hongo, Tomomaya Yamamoto, Tsuneyuki Yamamoto, Kimimitsu Oda, Paulo Henrique Luiz de Freitas, Minqi Li, Hidehiko Sano, Norio Amizuka
    BIOMEDICAL RESEARCH-TOKYO 38 4 257 - 267 2017年 [査読有り][通常論文]
     
    To assess the chronological participation of sclerostin and FGF23 in bone metabolism, this study tracked the immunolocalization of sclerostin and FGF23 in the metaphyses of murine long bones from embryonic day 18 (E18) through 1 day after birth, 1 week, 2 weeks, 4 weeks, 8 weeks, and 20 weeks of age. We have selected two regions in the metaphyseal trabeculae for assessing sclerostin and FGF23 localization: close to the chondro-osseous junction, i.e., bone modeling site even in the adult animals, and the trabecular region distant from the growth plate, where bone remodeling takes place. As a consequence, sclerostin-immunopositive osteocytes could not be observed in both close and distant trabecular regions early at the embryonic and young adult stages. However, osteocytes gradually started to express sclerostin in the distant region earlier than in the close region of the trabeculae. Immunoreactivity for FGF23 was observed mainly in osteoblasts in the early stages, but detectable in osteocytes in the later stages of growth in trabecular and cortical bones. Fgf23 was weakly expressed in the embryonic and neonatal stages, while the receptors, Fgfr1c and alpha Klotho were strongly expressed in femora. At the adult stages, Fgf23 expression became more intense while Fgfr1c and alpha Klotho were weakly expressed. These findings suggest that sclerostin is secreted by osteocytes in mature bone undergoing remodeling while FGF23 is synthesized by osteoblasts and osteocytes depending on the developmental/growth stage. In addition, it appears that FGF23 acts in an autocrine and paracrine fashion in fetal and neonatal bones.
  • Miyata Saori, Tanaka Saori, Matsuda Yasuhiro, Hashimoto Naoki, Sano Hidehiko, Kawanami Masamitsu
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 16 12 12996 - 13000 2016年12月 [査読有り][通常論文]
     
    Severe periodontal diseases cause root exposure, and there is no established method for the protection of exposed root surfaces. PRG Barrier Coat is a coating material with bioactive prereacted glass ionomer filler (hereafter, filler). However, the material can be detached in some clinical situations. We evaluated the effects of PRG Barrier Coat removal on root dentin demineralization under simulated oral cavity pH conditions. Five extracted human teeth were prepared, and three 200-mu m-thick single sections were obtained from each tooth. The polished surfaces, except the outer surface, were covered with a wax coating. PRG Barrier Coat (BC group) or Fuji III (FIII group), a glass ionomer cement, was applied to the root surfaces of one section each from each tooth and removed after 24 h. The remaining sections were included as a control group. Then, all specimens were subjected to pH cycling (pH 6.8-4.5) for 6 weeks. All specimens were observed using transverse microradiography images obtained weekly during pH cycling, which were used for integrated mineral loss assessments. Fluoride and calcium levels in each specimen were also evaluated at 5 weeks using a proton-induced gamma emission/proton-induced X-ray emission system. The control group surfaces and the BC and FIII group subsurfaces showed demineralization at 5 weeks, while an outer surface remineralization layer formed in the BC group. In the BC group, subsurface demineralization was observed at week 1, and it disappeared at week 6. There were no significant differences in IML values before and during pH cycling in the BC and FIII groups. Furthermore, fluorides penetrated the root surfaces of specimens in both groups. These findings suggest that PRG Barrier Coat and FIII supplemented the root surface with fluoride and inhibited demineralization, which can facilitate caries prevention for approximately 1 to 6 months.
  • Efficacy of a new filler-containing root coating material for dentin remineralization
    Katsushi Okuyama, Yoshitaka Kadowaki, Yasuhiro Matsuda, Naoki Hashimoto, Saiko Oki, Hiroko Yamamoto, Yukimichi Tamaki, Hidehiko Sano
    AMERICAN JOURNAL OF DENTISTRY 29 4 213 - 218 2016年08月 [査読有り][通常論文]
     
    Purpose: To evaluate a new root coating material containing surface pre-reacted glass-ionomer (S-PRG) filler for remineralization of demineralized dentin. Methods: The dentin was exposed on root surfaces of human third molars and demineralized by immersion in demineralization solution for 4 days. The demineralized dentin surface was divided into three areas. The center area was left untreated. The area on one side of the center area was coated with protective wax. The area on the other side was coated with one of four test materials: fluoride-containing S-PRG filler (PRG Barrier Coat: PR), fluoride-containing bonding agent (Bond Force: BF), fluoride-containing glass-ionomer cement as a positive control (Fuji IX EXTRA: EX), or non-fluoride-containing bonding agent as a negative control (Clearfil MegaBond: MB). The samples were stored in remineralization solution for 7 days, and then cut into two slices. The mineral changes, defined as variation in mineral loss between wax-coated area and the central untreated area, were measured in one slice by transversal microradiography. The fluoride concentration was measured in the other slice by mu-particle-induced gamma/X-ray emission. Seven thin specimens (0.25-mm thickness) of each test material were used to determine fluoride ion release from the materials over 21 days. Results: The mineral changes were greatest for EX, followed by PR, with no difference between BF and MB (P>0.05). Regarding the fluoride concentrations in dentin, there was no difference between EX and PR (P>0.05). MB had the lowest value (P<0.01). Fluoride release from EX was largest, followed by PR, with BF showing low fluoride release (P<0.05). MB had no fluoride release.
  • Yoshitaka Kadowaki, Shinichi Kakuda, Shimpei Kawano, Aiichiro Katsumata, Shihchun Ting, Shuhei Hoshika, Takatsumi Ikeda, Toru Tanaka, Ricardo Marinsde Carvalho, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 35 3 386 - 391 2016年06月 [査読有り][通常論文]
     
    The purpose of this study was to compare the micro-tensile bond strength (mu TBS) of three resin core composites to dentin and to examine the bonded interface of the composites. One experimental TDK-03(TD) and, two commercial, DC core Automix One (DC) and Unifil core EM(UN) were used. Flat dentin surfaces of human molars were exposed using #600 SiC paper and bonded with the respective adhesive of each system. After bonding, the composites were built up on the surfaces and cured under two conditions: "light condition" or "dark condition". mu TBSs (Wa) in the light condition were: TD; 60.02 +/- 17.08, DC; 38.21 +/- 13.70, and UN; 29.50 +/- 9.71; in the dark condition: TD; 54.62 +/- 17.11, DC; 8.40 +/- 4.81, and UN; 9.47 +/- 6.56. Dark curing negatively affected the bond strength of the two commercial resin-core materials. The experimental material was not affected by the curing conditions.
  • Pipop Saikaew, A. F. M. Almas Chowdhury, Mai Fukuyama, Shinichi Kakuda, Ricardo M. Carvalho, Hidehiko Sano
    JOURNAL OF DENTISTRY 47 63 - 70 2016年04月 [査読有り][通常論文]
     
    Objective: This study evaluated the effects of surface preparation and the application time of adhesives on the resin-dentine bond strengths with universal adhesives. Methods: Sixty molars were cut to exposed mid-coronal dentine and divided into 12 groups (n = 5) based on three factors; (1) adhesive: G-Premio Bond (GP, GC Corp., Tokyo, Japan), Clearfil Universal Bond (CU, Kuraray Noritake Dental Inc., Okayama, Japan) and Scotchbond Universal Adhesive (SB, 3M ESPE, St. Paul, MN, USA); (2) smear layer preparation: SiC paper ground dentine or bur-cut dentine; (3) application time: shortened time or as manufacturer's instruction. Fifteen resin-dentine sticks per group were processed for microtensile bond strength test (mu TBS) according to non-trimming technique (1 mm(2)) after storage in distilled water (37 degrees C) for 24 h. Data were analyzed by three-way ANOVA and Dunnett T3 tests (alpha = 0.05). Fractured surfaces were observed under scanning electron microscope (SEM). Another 12 teeth were prepared and cut into slices for SEM examination of bonded interfaces. Results: mu TBS were higher when bonded to SiC-ground dentine according to manufacturer's instruction. Bonding to bur-cut dentine resulted in significantly lower mu TBS (p < 0.000). Shortening the application time resulted in significantly lower bond strength for CU on SiC and GP on bur-cut dentine. SEM of fractured surfaces revealed areas with a large amount of porosities at the adhesive resin interface. This was more pronounced when adhesives were bonded with a reduced application time and on bur cut dentine. (C) 2016 Elsevier Ltd. All rights reserved.
  • Shizuma Shibata, Luiz Clovis Cardoso Vieira, Luiz Narciso Baratieri, Jiale Fu, Shuhei Hoshika, Yasuhiro Matsuda, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 35 2 166 - 173 2016年04月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the mu TBS (microtensile bond strength) of currently available self-etching adhesives with an experimental self-etch adhesive in normal and caries-affected dentin, using a portable hardness measuring device, in order to standardize dentin Knoop hardness. Normal (ND) and caries-affected dentin (CAD) were obtained from twenty human molars with class II natural caries. The following adhesive systems were tested: Mega Bond (MB), a 2-step self-etching adhesive; MTB-200 (MTB), an experimental 1-step self-etching adhesive (1-SEA), and two commercially available one-step self-etching systems, G-Bond Plus (GB) and Adper Easy Bond (EB). MB-ND achieved the highest mu TBS (p<0.05). The mean mu TBS was statistically lower in CAD than in ND for all adhesives tested (p<0.05), and the 2-step self-etch adhesive achieved better overall performance than the 1-step self-etch adhesives.
  • Eri Seitoku, Shigeaki Abe, Teruo Kusaka, Mariko Nakamura, Satoshi Inoue, Yasuhiro Yoshida, Hidehiko Sano
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 16 4 3373 - 3377 2016年04月 [査読有り][通常論文]
     
    In this study, we investigated the behaviors and cytocompatibility response of human cervical carcinoma (HeLa) cells expose to nano-sized particles. Cultivated cells exposed to titanium oxide and indium oxide nanoparticles remained highly viable. In the presence of copper oxide (CuO); however, the cells became seriously inflamed. To understand the mechanism by which CuO causes cell death, we evaluated cell death and apoptosis cytometry. CuO induced cells apoptosis more strongly than exposure to titania nanoparticles. Confocal fluorescence microscopy revealed that the nano-sized particles penetrate the cells.
  • Shigeaki Abe, Eri Seitoku, Nobuki Lwadera, Yusuke Hamba, Shuichi Yamagata, Tsukasa Akasaka, Teruo Kusaka, Satoshi Lnoue, Yasutaka Yawaka, Junichiro Lida, Hidehiko Sano, Tetsu Yonezawa, Yasuhiro Yoshida
    JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 12 3 472 - 480 2016年03月 [査読有り][通常論文]
     
    We assessed the biocompatibility of nano-sized ceramic particles with several cells types. Though these particles have less than 100 nm in diameter, they act as submicron-sized particles in saline by aggregation that was estimated using laser diffraction particle size analysis (LDS). they act as submicro-sized particles in saline by aggregation based on laser diffraction particle size analysis (LDS). Several types of cells (osteoblasts, osteosarcoma and hepatocyte cells) were exposed to these particles and their cytocompatibility was estimated. Not only the cytotoxic assay but also their static and dynamic morphology under nanoparticles exposure were investigated. The intercellular uptake of particles was determined using a confocal fluorescence microscope. The particles used in this study did not inhibit cellular activity or growth even when their concentrations were high. Only copper oxide particles caused acute cytotoxicity depending on the particle size. The cytotoxicity assay, dynamic behavior of the nanoparticle-exposed cells and their examination under a confocal fluorescence microscope suggests that the irritative reaction was induced by contact between the cells and particles, whereas eluted copper ions are not dominant factor. These results indicate that nano-sized particles used in this study have excellent biocompatibility except copper oxide ones.
  • 勝俣 愛一郎, 門脇 佳孝, 川野 晋平, 丁 世俊, 角田 晋一, 星加 修平, 池田 考績, 田中 享, 佐野 英彦
    日本歯科保存学雑誌 59 1 32 - 39 (NPO)日本歯科保存学会 2016年02月 [査読有り][通常論文]
  • Shinichi Kakuda, Sharanbir K. Sidhu, Hidehiko Sano
    JOURNAL OF DENTISTRY 43 10 1285 - 1289 2015年10月 [査読有り][通常論文]
     
    Objectives: The aim of this study was to evaluate the buffering capacity of glass-ionomer material in vitro. The null hypothesis tested was that there is no effect of cured glass-ionomer pit-and-fissure sealant (PFS) pastes on the environmental acidity as well as the tooth substrate. Method: For each material, a cured PFS disk and a section of human enamel were simultaneously soaked in lactic acid solution in a conical tube, and the pH of the solution was measured daily for one week. Subsequently, the total amount of calcium leached out in solution was also measured with ICP-AES. Results: The results showed that the acidity of the solutions changed over time. Significant differences of calcium ion concentration in solution were observed as a result of decalcification. As the PFS products tested did not include calcium, the concentration of calcium ion released indicated acidic erosion of the tooth enamel. Conclusions: The glass-ionomer countered the acid of the solution rapidly and preserved the structure of human tooth enamel. (C) 2015 Elsevier Ltd. All rights reserved.
  • Matsuda Yasuhiro, Okuyama Katsushi, Yamamoto Hiroko, Komatsu Hisanori, Koka Masashi, Sato Takahiro, Hashimoto Naoki, Oki Saiko, Kawamoto Chiharu, Sano Hidehiko
    NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS 348 156 - 159 2015年04月 [査読有り][通常論文]
     
    To prevent the formation of caries and reduce dentin hypersensitivity, sealing materials, either with or without fluoride, are generally applied on the tooth in clinical practice. Application of-fluoride-free sealing materials results in the formation of an acid-resistant layer on the tooth surface. On the other hand, fluoride-containing sealing materials might not only form an acid-resistant layer but could possibly further provide fluoride to enhance remineralization and reduce demineralization. In this study, the demineralization prevention ability and fluorine uptake rate in human enamel of fluoride-containing sealing materials ["MS coats F" (MSF)] and fluoride-free sealing materials ("hybrid coats 2" [HI]) were evaluated using an automatic pH cycling system. Each material was applied to the original tooth surface, the cut surfaces were covered with sticky wax, and the automatic pH-cycling system simulated daily acid changes (pH 6.8-4.5) occurring in the oral cavity for 4 weeks. Caries progresion was analyzed using transverse microradiography (TMR) taken pre and post the 4 weeks of pH cycling. The fluorine and calcium distributions in the carious lesion in each specimen were evaluated using the proton-induced gamma emission (PIGE) and proton-induced X-ray (PIXE) techniques, respectively. TMR analysis showed that both MSF and HI had a caries-preventing effect after 4 weeks of pH cycling. PIGE/PIXE analysis demonstrated that only MSF resulted in fluoride uptake in the enamel surface. Therefore, MSF can help to form an acid-resistant layer and provide fluoride to the enamel surface. The presence of fluoride on the enamel surface suggested that MSF could prevent demineralization, even if the acid-resistant layer was removed, in clinical settings. The data obtained using the PIGE and PIXE techniques are useful for understanding the benefits of the use of a fluoride-containing sealing material for preventing caries. (C) 2015 Elsevier B.V. All rights reserved.
  • 1ステップ型接着システムによる臨床的スミヤー層の除去能とその接着性能
    福澤 尚幸, 福岡 杏理, 角田 晋一, 池田 考績, 田中 享, 井上 哲, 佐野 英彦
    接着歯学 33 1 6 - 16 (一社)日本接着歯学会 2015年04月 [査読有り][通常論文]
  • Shihchun Ting, A. F. M. Almas Chowdhury, Feng Pan, Jiale Fu, Jihow Sun, Shinichi Kakuda, Shuhei Hoshika, Yasuhiro Matsuda, Takatsumi Ikeda, Yasuko Nakaoki, Shigeaki Abe, Yasuhiro Yoshida, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 34 2 181 - 188 2015年04月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the effect of remaining dentin thickness (RDT) on the bond strength of current adhesive systems. Third molars were randomly allocated among four groups depending on the adhesive system used: Clearfil SE Bond ONE (SE1), G-Bond PLUS (GB), BeautiBond (BB), and Clearfil Mega Bond (MB). Bonded specimens were stored in water at 37 C for 24 h. Teeth were then sectioned perpendicular to the adhesive interface to produce beams. After measuring RDT of each beam, microtensile bond strength test was carried out using a universal testing machine at a crosshead speed of 1 mm/min. All data were analyzed by linear regression analysis. Bond strengths of one-step self-etch materials used in this study increased with an increase in RDT. In contrast, that of two-step self-etch adhesive system was not affected by RDT.
  • Yoshiki Funato, Yasuhiro Matsuda, Katsushi Okuyama, Hiroko Yamamoto, Hisanori Komatsu, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 34 2 240 - 245 2015年04月 [査読有り][通常論文]
     
    Fluorine (F) and strontium (Sr) are key elements in the de- and remineralization of teeth. To quantitatively analyze the distribution of F and Sr, micro-particle-induced gamma/X-ray emission (PIGE/PIXE) technique was used. The cavities were prepared and filled with the fluoride- and Sr-containing restorative materials (FSCMs) in extracted human molars. The single-section enamel specimens were prepared by slicing from the buccal to lingual surface including the FSCMs. After 5 weeks of automatic pH cycling, the demineralization was calculated by integrated mineral loss (Delta IML) from transverse-microradiography. The distributions of F and Sr were analyzed by the PIGE/PIXE technique. The micro-PIGE/PIXE technique indicated a fluorine uptake difference between the enamel surface and enamel cavity wall. Delta IML of FSCMs were significantly lower than intact enamel. The micro-PIGE/PIXE technique enables measurement of F and Sr uptake from FSCMs into enamel, which would be beneficial for research on caries development and prevention.
  • Shimpei Kawano, Jiale Fu, Pipop Saikaew, Afm Almas Chowdhury, Naoyuki Fukuzawa, Yoshitaka Kadowaki, Shinichi Kakuda, Shuhei Hoshika, Yasuko Nakaoki, Takatsumi Ikeda, Toru Tanaka, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 34 1 61 - 69 2015年02月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the microtensile bond strength (mu TBS) of a newly developed resin cement, ECD-89 (ECD, Tokuyama Dental) to dentin and to observe the interfacial micromorphology by comparing with two commercial resin cements, Multilink Automix (MA, Ivoclar Vivadent AG, Schaan, Liechtenstein) and Panavia F2.0 (PF, Kuraray Noritake Dental). Flat dentin surfaces of human third molars were exposed using #600 SiC. After application of primer and cement to the dentin surface, each cement was applied and cured with light (light condition) or without light. (dark condition). The teeth were sectioned to obtain beams (1 mmx1 mm) after 24 h of water storage. The mean bond strengths and SDs (MPa) were: ECD: 68.6 +/- 14.9, MA: 39.2 +/- 18.9, PF: 39.4 +/- 18.5 and ECD: 54.5 +/- 22.4, MA: 36.7 +/- 15.6, PF: 13.4 +/- 4.46 when cured in light and dark condition, respectively. In both conditions, ECD-89 showed statistically higher mu TBS than the others.
  • Shuhei Hoshika, Jan De Munck, Hidehiko Sano, Sharan Kabir Sidhu, Bart Van Meerbeek
    JOURNAL OF ADHESIVE DENTISTRY 17 2 141 - 146 2015年 [査読有り][通常論文]
     
    Purpose: To determine the bond stability and the change in interfacial ultrastructure of a conventional glass-ionomer cement bonded to dentin, with and without pretreatment using a polyalkenoic acid conditioner. Materials and Methods: The occlusal dentin surfaces of 10 teeth were ground flat. Glass-ionomer cement was bonded to the surfaces either with or without polyalkenoic acid conditioning. The teeth were sectioned into 1-mm(2) stick-shaped specimens. The 200 specimens obtained were randomly assigned to four groups with different periods of storage in water: 1 week, 1 month, 3 months, and 6 months. The microtensile bond strength (mu TBS) was determined for each storage time. Additional specimens were prepared for transmission electron microscopy (TEM); they were produced with or without prior polyalkenoic acid conditioning in the same manner as for the mu TBS test. Results: There was no significant difference in mu TBS to conditioned dentin (p > 0.05). After 6 months of aging, the mu TBS to non-conditioned dentin was significantly reduced as compared to the 1-week, 1-month, and 3-month results (p < 0.05). The failures appeared to be of a mixed nature, although aging caused more areas of cohesive than adhesive failure in all groups. TEM observation showed a demineralized layer and an amorphous gel phase in the polyalkenoic acid conditioned group. Conclusion: Aging did not reduce the bond strength of the conventional glass-ionomer cement to dentin when the surface was pretreated with a polyalkenoic acid conditioner.
  • Masayuki Kaga, Shinichi Kakuda, Yusuke Ida, Hirokazu Toshima, Masanori Hashimoto, Kazuhiko Endo, Hidehiko Sano
    EUROPEAN JOURNAL OF ORAL SCIENCES 122 1 78 - 83 2014年02月 [査読有り][通常論文]
     
    The buffering capacity and inhibitory effects on enamel demineralization of two commercially available dental sealants were evaluated in this study. The effects of filler particles were also examined. Disks of enamel and cured sealant materials of BeautiSealant (silica or S-PRG filler) or Teethmate F-1 were incubated in lactic acid solutions (pH 4.0) for 1-6 d. The pH changes and amounts of ions released in the solutions were assessed, and enamel surfaces were observed using a scanning electron microscope. The pH of the solution with BeautiSealant (S-PRG filler) was neutralized from pH 4.0 to pH 6.1 (after incubation for 1 d) and from pH 4.0 to pH 6.7 (after incubation for 6 d). In addition, no release of calcium ions was detected and the enamel surface was morphologically intact in scanning electron microscopy images. However, the pH of the solution with Teethmate F-1 remained below pH 4.0 during incubation from days 1 to 6. Calcium release was increased in solutions up to and after 6 d of incubation. Scanning electron microscopy images showed that the structures of hydroxyapatite rods were exposed at the specimen surfaces as a result of demineralization. Ions released from S-PRG filler-containing dental sealant rapidly buffered the lactic acid solution and inhibited enamel demineralization.
  • Shinichi Kakuda, Jiale Fu, Yasuko Nakaoki, Takatsumi Ikeda, Toru Tanaka, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 32 4 600 - 607 2013年08月 [査読有り][通常論文]
     
    The aim of this study was to determine the performance of an experimental all-in-one adhesive. The adhesive, named MTB-200 (Kuraray Medical), contained components to enhance both bond strength and hydrophobicity. The performance of the adhesive was compared with that of CLEARFIL TRI-S BOND (Kuraray Medical) and BeautiBond (SHOFU) using micro-tensile bond strength test and ultramicroscopic observations. The study revealed that the new adhesive had the highest tensile strength value among the three adhesives over time, although transmission electron microscopic images showed the phenomenon of filler de-bonding in the adhesive resin layer. In spite of modification in the experimental adhesive, the adhesive was suspected to degrade bond performance. However, revision of the composition of adhesives would be one of the solutions to enhance durability of interface.
  • Jiale Fu, Shinichi Kakuda, Feng Pan, Shuhei Hoshika, Shihchun Ting, Anri Fukuoka, Yang Bao, Takatsumi Ikeda, Yasuko Nakaoki, Denis Selimovic, Hidehiko Sano, Sharanbir K. Sidhu
    DENTAL MATERIALS JOURNAL 32 2 203 - 211 2013年04月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the short-term mu TBS (Micro-tensile bond strength) and microscopic (SEM and TEM) observation of four recent adhesives. One adhesive was an experimental step-less 1-step system (LLB-2, Tokuyama Dental), which is an all-in-one system without the light-curing step in the application process. The other two were self-adhering light-cured flowable composite resin systems FLD (Fusio Liquid Dentin, Pentron Clinical Technologies) and VF (Vertise Flow Dental Restorative Materials, Kerr Corporation), which combine all the bonding steps together. A 2-step self-etching system MG (Clearfil MegaBond, Kuarary Medical) was employed as the control group in this study. The mu TBS of MG was the highest (79.0 MPa) followed by that of LLB-2 (63.1 MPa), FLD (23.6 MPa), and VF (13.1 MPa). The microscopic observations showed that MG and LLB-2 had an approximately 20 mu m and 5 mu m adhesive layer respectively, without bubble or gap-formation at the resin-dentin interface, which were found in FLD and VF.
  • Effective Screening for the HLA-B*35:05 and HLA-B*57:01 Alleles by Using an Allele-Specific Polymerase Chain Reaction Melting Assay
    Yasuhiro Matsuda, Hidehiko Sano, Yuichi Iwaki
    INTERNATIONAL CONFERENCE ON BIOLOGICAL, MEDICAL AND CHEMICAL ENGINEERING (BMCE 2013) 160 - 168 2013年 [査読有り][通常論文]
     
    Specific alleles of the human leukocyte antigen(HLA)-Bgene (HLA-B*35:05 and HLA-B*57:01) are associated with adverse reactions to the human immunodeficiency virus (HIV) medications abacavir and nevirapine. This fact underscores the need for rapid and cost-effective methods for the screening of these 2 alleles. Allele-specific polymerase chain reaction melting temperature analysis (AS-PCR-TmA) followed by DNA sequence analysis were performed to confirm the presence of HLA-B*35:05 and HLA-B*57:01by using 8 positive HLA-B*35:05 and 9 positive HLA-B*57:01samples. Next, 193 samples were analyzed blindly by this method; 8 samples containing HLA-B*35:05 and a total of 22 samples containing HLA-B*57:01 were detected by AS-PCR-TmA. The results were confirmed by DNA sequencing. None of the negative samples showed false-negative results, as confirmed by LAB Type sequence-specific oligonucleotide HLA typing and microsequence-specific primer HLA typing. The screening method demonstrated 100% sensitivity and specificity for the detection of HLA-B*35:05 and HLA-B*57:01, which indicates the suitability of this protocol in clinical settings.
  • Jiale Fu, Feng Pan, Shinichi Kakuda, Sharanbir K. Sidhu, Takatsumi Ikeda, Yasuko Nakaoki, Denis Selimovic, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 31 6 1075 - 1081 2012年11月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the effect of air-blowing duration on the bonding performance of all-in-one systems using the same pressure (0.25 MPa). Three all-in-one systems were: EB (Easy Bond, 3M ESPE, USA), BB (BeautiBond, Shofu Inc., Japan) and GBp (G-Bond plus, GC Corporation, Japan). Alter adhesive application, the 3 systems were air-blown thereafter using 7 different durations (5 s, 10 s, 15 s, 20 s, 25 s, 30 s and 35 s). Bond strengths to dentin were determined using mu TBS test after 24 h water storage. In addition, evaluation of both the resin-dentin interface and the fractured surface on the dentin side were performed by SEM. The maximum mu TBS for each system, BB (40.4 +/- 14.8 MPa), EB (79.8 +/- 16.5 MPa), and GBp (47.3 +/- 17.6 MPa), were recorded with 15 s, 15 s and 25 s air-blowing duration respectively. Under the same air-pressure, the air-blowing duration could affect evaporation and the thickness of the adhesive layer, which contributed to the different bond strengths.
  • 五十嵐豊, FU Jiale, 角田晋一, 田中享, 中沖靖子, 佐野英彦
    北海道歯学雑誌 32 2 177 - 192 2012年03月 [査読有り][通常論文]
  • Mayumi Nomura, Yoshitaka Yoshimura, Takashi Kikuiri, Tomokazu Hasegawa, Yumi Taniguchi, Yoshiaki Deyama, Ken-ichi Koshiro, Hidehiko Sano, Kuniaki Suzuki, Nobuo Inoue
    JOURNAL OF PHARMACOLOGICAL SCIENCES 117 4 243 - 252 2011年12月 [査読有り][通常論文]
     
    Recent research has shown that platinum nanoparticles (nano-Pt) efficiently quench reactive oxygen species (ROS) as a reducing catalyst. ROS have been suggested to regulate receptor activator of NF-kappa B ligand (RANKL)-stimulated osteoclast differentiation. In the present study, we examined the direct effects of platinum nano-Pt on RANKL-induced osteoclast differentiation of murine pre-osteoclastic RAW 264.7 cells. The effect of the nano-Pt on the number of osteoclasts was measured and their effect on the mRNA expression for osteoclast differentiation was assayed using real-time PCR. Nano-Pt appeared to have a ROS-scavenging activity. Nano-Pt decreased the number of osteoclasts (2+ nuclei) and large osteoclasts (8+ nuclei) in a dose-dependent manner without affecting cell viability. In addition, this agent significantly blocked RANKL-induced mRNA expression of osteoclastic differentiation genes such as c-fms, NFATc 1, NFATc2, and DC-STAMP as well as that of osteoclast-specific marker genes including MMP-9, Cath-K, CLC7, ATP6i, CTR, and TRAP. Although nano-Pt attenuated expression of the ROS-producing NOX-family oxidases, Nox1 and Nox4, they up-regulated expression of Nox2, the major Nox enzyme in macrophages. These findings suggest that the nano-Pt inhibit RANKL-stimulated osteoclast differentiation via their ROS scavenging property. The use of nano-Pt as scavengers of ROS that is generated by RANKL may be a novel and innovative therapy for bone diseases.
  • Shuhei Hoshika, Futami Nagano, Toru Tanaka, Takahiro Wada, Kiyotaka Asakura, Kenichi Koshiro, Denis Selimovic, Yusei Miyamoto, S. K. Sidhu, Hidehiko Sano
    JOURNAL OF ADHESIVE DENTISTRY 13 5 411 - 416 2011年10月 [査読有り][通常論文]
     
    Purpose: To investigate the effect of Colloidal Platinum Nanoparticles (CPN) on the bond strength between dentin and 4-META/MMA-TBB resin using different concentrations of CPN. Materials and Methods: Twenty-five extracted human third molars were stored in 0.5% chloramine T. The occlusal dentin slices were prepared by grinding occlusal surfaces of each tooth and polishing with 600-grit silicon carbide paper under running water. One control and four experimental groups (2 specimens per group) were used as follows: a) dentin surfaces treated with 10-3 solution, followed by rinsing with water and subsequently an acrylic rod bonded with hand-mixed 4META/MMA-TBB resin (Super-Bond C&B, Sun Medical) (control); b) dentin surfaces treated with 10-3 etching solution, followed by rinsing with water and application of CPN (100% or 10%) as a primer solution for 60 s and rinsed with water for 20 s, then an acrylic rod bonded with Super-Bond C&B(Etch-CPN [100% or 10%]); c) dentin surfaces treated with CPN (100% or 10%) for 60 s, rinsed with water for 20 s, followed by application of 10-3 solution, then an acrylic rod bonded with Super-Bond C&B (CPN-Etch [100% or 10%]). After storage in 37 C water, specimens were sectioned into beams (cross-sectional area: 1 mm(2)) for microtensile bond strength testing at a crosshead speed of 1mm/min. The data were analyzed using the Games-Howell method (p < 0.05; n = 15). Results: Etch-CPN (100), CPN-Etch(100) and CPN-Etch (10) showed significantly higher bond strengths compared to the control. When using 10% CPN, the highest bond strength was demonstrated. The bond strength of 4META/MMA-TBB resin was approximately doubled by CPN application. Conclusion: The results of this study showed that higher bond strengths are obtained when treating dentin with a lower concentration of CPN. Further evaluation to optimize conditions such as the application time and rinsing time are required.
  • Katsushi Okuyama, Hisanori Komatsu, Hiroko Yamamoto, Patricia N. R. Pereira, Ana K. Bedran-Russo, Masaharu Nomachi, Takahiro Sato, Hidehiko Sano
    NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS 269 20 2269 - 2273 2011年10月 [査読有り][通常論文]
     
    The use of fluoride for the prevention of caries is based on the transformation of hydroxylapatite to fluoroapatite in the presence of fluoride ions, thereby strengthening tooth structure. Adhesion of dentin and resin composite (tooth-colored restoration material) requires a dentin bonding system, since resin composite is not able to adhere to dentin directly. Demineralization of dentin by acid etching is an important step in the dentin bonding system, however, demineralization also introduces weaknesses in tooth structure. If the demineralized dentin could be strengthened by the application of fluoride, then the dentin-resin composite bond strength might also improve. To test this hypothesis, the present study evaluated the influence of fluoride applications on the strength of the dentin-resin composite bond by (1) tensile strength testing analyses, (2) SEM analyses of tooth structure, and (3) detection of calcium (Ca) and fluorine (F) distribution patterns by micro proton-induced X-ray emission (mu-PIXE) and micro proton-induced gamma-ray emission (mu-PIGE) analyses conducted at the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) at the Takasaki Advanced Radiation Research Institute (TARRI). In this study, the dentin in extracted human molars was exposed by grinding and the dentin was etched with 35% phosphoric acid. Fluoride was applied at two concentrations, 0.022% (100 ppm F) and 2.21% (10,000 ppm F) NaF solution, for two time periods, 30 and 60 s, prior to bonding the resin composite with the treated dentin. Controls were prepared in the same manner, but without the fluoride application. Bond strength was measured with a micro-tensile testing unit, and the fluorine and calcium distributions at the interface between dentin and resin composite were detected by mu-PIGE and mu-PIXE analysis, respectively. Results indicate that the 10,000 ppm F applications resulted in higher bond strengths than observed in either the 100 ppm F applications or the control group. In addition, PIGE analyses showed high concentrations of fluorine in the hybrid bonding layer of the 10,000 ppm F samples, suggesting that the fluorine contributes to the strength of the dentin-resin composite bond. Detection of fluoroapatite within the hybrid bonding layer suggests that bond strength involves remineralization processes. (C) 2011 Elsevier B.V. All rights reserved.
  • Anri Fukuoka, Kenichi Koshiro, Satoshi Inoue, Yasuhiro Yoshida, Toru Tanaka, Takatsumi Ikeda, Kazuomi Suzuki, Hidehiko Sano, Bart Van Meerbeek
    JOURNAL OF ADHESIVE DENTISTRY 13 3 243 - 248 2011年06月 [査読有り][通常論文]
     
    Purpose: To evaluate the hydrolytic stability of three one-step self-etching adhesives (1-SEAs) bonded to dentin through bond strength testing and ultra-morphological interfacial analysis before and after long-term thermocycling. Materials and Methods: Eighteen flattened mid-coronal dentin surfaces of extracted human molars were subjected to bonding treatment using three 1-SEAs (Clearfil S(3) Bond, Kuraray (S3), G-Bond, GC (GB), Absolute, Dentsply-Sankin (AB)), after which the bonded surfaces were built up with composite. After storage overnight at 37 C, the specimens were sectioned into slabs and further trimmed into an hourglass shape with an interface area of approximately 1 mm(2). The specimens were left untouched (control) or were thermocycled for 100,000 cycles. The microtensile bond strength (mu TBS) was measured and the ultrastructure of the adhesive/dentin interface characterized using transmission electron microscopy (TEM). Results: Long-term thermocycling significantly decreased the mu TBS of all one-step adhesives tested (p < 0.05, one-way ANOVA and Games-Howell test). TEM revealed a similar interfacial ultrastructure before and after thermocycling for S3. For GB, many voids were observed at the interface after 100,000 thermocycles. Regarding AB, collagen fibrils could no longer be clearly observed upon staining, while the adjacent unaffected dentin was rich in voids. Conclusion: The bond strength and ultramorphological data demonstrated that the bond of 1-SEAs to dentin degrades with time, although the degree of degradation is obviously material dependent.
  • Hanako Kachi, Mamoru Noda, John C. Wataha, Yasuko Nakaoki, Hidehiko Sano
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 96B 2 193 - 198 2011年02月 [査読有り][通常論文]
     
    Components of dental composites have been implicated as metabolic and oxidative stressors in cells. Antioxidant colloidal platinum nanoparticles (CPtN) could be useful as nanofillers that reduce cellular stress caused during placement and exposure to unpolymerized composite components. Yet, CPtN may have pro- or anti-oxidative properties depending on the chemical environment in which they are used. This study investigated the ability of CPtN to mitigate mitochondrial or oxidative stress induced by camphorquinone (CO) and dimethylaminoethyl methacrylate (DMAEM), two components of dental composites that initiate light-activated addition polymerization. THP-1 human monocytic cells were exposed to subtoxic concentrations of CO and DMAEM, then blue light for 0-60 s. Mitochondrial and oxidative stress were estimated 0, 6, or 24 h after irradiation by measuring cellular ATP or GSH levels, respectively. CPtN (0-20 ppm) were added prior to blue light irradiation. Statistically significant effects were determined by ANOVA with Tukey post-hoc analysis (alpha = 0.05). Light alone did not alter THP-1 activity, but CO and DMAEM induced 120% increases in cell ATP levels, which was increased further (50%) by CPtN. Blue light irradiation caused a 50% drop in ATP levels, which increased to 75% when CPtN were added. GSH levels were suppressed by CQ/DMAEM and blue light, and CPtN increased suppression by 20-30%. CPtN did not mitigate mitochondrial or oxidative stress in THP-1, but amplified these stresses under the conditions tested. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 96B: 193-198, 2011.
  • Martine Soell, Joseph Hemmerle, Matthias Hannig, Youssef Haiekel, Hidehiko Sano, Denis Selimovic
    EUROPEAN JOURNAL OF ORAL SCIENCES 118 6 590 - 595 2010年12月 [査読有り][通常論文]
     
    Viridans streptococci possess a family of immunologically and structurally related cell-surface proteins, termed antigen I/II, which may function as adhesins and enable oral streptococci to adhere to saliva-coated surfaces and matrix proteins. Here we used atomic force microscopy in the molecular force mode to measure the specific interaction forces between antigen I/II and two matrix proteins, collagen and fibronectin. These matrix proteins provide important binding sites for adherence of oral streptococcal in dentinal caries and endocarditis, respectively. Antigen I/II-coated cantilever tips were brought into contact with collagen- or fibronectin-coated silica coverslips. For the protein I/II-fibronectin interaction experiments, the mean strength of the last ruptures was 216 pN, with most of the detachments located around 125 pN. In antigen I/II-collagen interaction experiments, the mean strength of the last rupture forces corresponded to 136 pN, with the most frequent unbinding force around 75 pN. Thus, our findings definitely suggest that, under the present experimental conditions, antigen I/II binds more strongly to fibronectin than to type I collagen. This might be of relevance for the attachment of viridians streptococci to surfaces exposed to strong hydrodynamic shearing forces under in vivo conditions.
  • Shuhei Hoshika, Futami Nagano, Toru Tanaka, Takatsumi Ikeda, Takahiro Wada, Kiyotaka Asakura, Kenichi Koshiro, Denis Selimovic, Yusei Miyamoto, Sk Sidhu, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 29 6 682 - 689 2010年11月 [査読有り][通常論文]
     
    The purpose of this study was to investigate the effect of application time of colloidal platinum nanoparticles (CPN) on bond strength. Dentin surfaces were subjected to one of the following treatments: (A) Etching with 10% citric acid-3% FeCl3 solution (10-3 solution); (B) Etching with 10-3 solution followed by applying CPN as a primer solution for 10, 20, 30, or 60 seconds; and (C) Priming with CPN for 10, 20, 30, or 60 seconds followed by etching with 10-3 solution. An acrylic rod was bonded to each treated dentin surface using 4-META/MMA-TBB resin. Bonded specimens were sectioned into beams for microtensile bond strength testing. In groups (B) and (C), highest bond strength was obtained when dentin surfaces were treated with CPN for 30 seconds. This meant that the CPN primer solution either enhanced the penetration of resin into dentin or the degree of conversion of 4-META/MMA-TBB resin. Within the limitations of this study, treatment with 0.1 mN CPN primer solution followed by 20 seconds of water rinsing resulted in high bond strength.
  • Shuhei Hoshika, Futami Nagano, Toru Tanaka, Takatsumi Ikeda, Takahiro Wada, Kiyotaka Asakura, Kenichi Koshiro, Denis Selimovic, Yusei Miyamoto, S K Sidhu, Hidehiko Sano
    Dental Materials Journal 29 6 682 - 689 2010年11月 [査読無し][通常論文]
     
    The purpose of this study was to investigate the effect of application time of colloidal platinum nanoparticles (CPN) on bond strength. Dentin surfaces were subjected to one of the following treatments: (A) Etching with 10% citric acid-3% FeCl(3 )solution (10-3 solution); (B) Etching with 10-3 solution followed by applying CPN as a primer solution for 10, 20, 30, or 60 seconds; and (C) Priming with CPN for 10, 20, 30, or 60 seconds followed by etching with 10-3 solution. An acrylic rod was bonded to each treated dentin surface using 4-META/MMA-TBB resin. Bonded specimens were sectioned into beams for microtensile bond strength testing. In groups (B) and (C), highest bond strength was obtained when dentin surfaces were treated with CPN for 30 seconds. This meant that the CPN primer solution either enhanced the penetration of resin into dentin or the degree of conversion of 4-META/MMA-TBB resin. Within the limitations of this study, treatment with 0.1 mN CPN primer solution followed by 20 seconds of water rinsing resulted in high bond strength.
  • FU Jiale, 角田晋一, 田中享, 佐野英彦
    接着歯学 28 1 28 - 32 2010年04月 [査読有り][通常論文]
  • 長野 二三, 橋本 正則, 佐野 英彦, 星加 修平, 井田 有亮, 大野 弘機, 遠藤 一彦
    日本歯科理工学会誌 29 2 176 - 176 (一社)日本歯科理工学会 2010年03月 [査読有り][通常論文]
  • Soell M, Feki A, Hannig M, Sano H, Pinget M, Selimovic D
    Bosnian journal of basic medical sciences / Udruzenje basicnih mediciniskih znanosti = Association of Basic Medical Sciences 10 1 2 - 8 1 2010年02月 [査読有り][通常論文]
  • Hongxia Wu, Mikako Hayashi, Kenji Okamura, Evgeni V. Koytchev, Satoshi Imazato, Saori Tanaka, Yuko Tanaka, Hidehiko Sano, Shigeyuki Ebisu
    DENTAL MATERIALS 25 12 1484 - 1492 2009年12月 [査読有り][通常論文]
     
    Objectives. This study investigated the effects of light penetration and removal of smear layer on the adhesive properties of self-etching adhesives to root canal dentin when using fiber posts. Methods. Altogether 54 human incisors and premolars were decoronated, and the roots after preparing the post space were randomly assigned into six groups. Three groups were treated with a light-cured self-etching adhesive and the other three with a dual-cured self-etching adhesive. In a further sub-division, the first group was light-cured with an irradiation unit; the second was light-cured with the irradiation unit and a light-guiding attachment; and the third was light-cured with the irradiation unit and the attachment after removing smear layer by EDTA and NaOCl. Then, a glass-fiber post was luted into the post space by a dual-cured composite. After 24 h, an hourglass-shaped specimen was trimmed and subjected to MTBS testing. Results. Using the light-guiding attachment and removing the smear layer reduced the incidence of pre-testing failure significantly from 57.1% to 19.0% (p < 0.05) in the light-cured adhesive groups and from 68.3% to 3.2% (p < 0.01) in the dual-cured adhesive groups. The MTBS in different locations within a post space were in the range from 8.9 to 17.5 MPa in the light-cured group and from 11.2 to 17.2MPa in the dual-cured group. Significance. Better light penetration in post spaces and removal of the smear layer are effective in improving the adhesive properties of self-etching adhesives to root canal dentin when using fiber posts. (C) 2009 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • 星加修平, 安本恵, 長野二三, 田中享, 佐野英彦
    接着歯学 27 2 84 - 88 2009年08月 [査読有り][通常論文]
  • Effect of light curing and dark reaction phases on the thermomechanical properties of a Bis-GMA based dental restorative material
    Roberto De Santis, Antonio Gloria, Hidehiko Sano, Eugenio Amendola, Davide Prisco, Francesco Mangani, Sandro Rengo, Luigi Ambrosio, Luigi Nicolais
    JOURNAL OF APPLIED BIOMATERIALS & BIOMECHANICS 7 2 132 - 140 2009年05月 [査読有り][通常論文]
     
    Purpose: The effects of light curing units (LCU) and energy doses on the chemical and physical properties of a dental composite were investigated. Methods: The effects on the chemical and physical properties of a bisphenol A diglycidylether methacrylate (Bis-GMA) based dental restorative material were evaluated through photospectrometry, differential scanning calorimetry, and mechanical measurements. Results: The light curing conditions associated with direct and indirect restorations were replicated in vitro using optical investigation techniques. A slight attenuation resulted independently of the LCU and a strong attenuation was measured for the cement luting a thick inlay, as well as for the deepest layer of a composite filling increment. Calorimetric measurements indicated that the curing degree is very sensitive to the light energy dose rather than to the LCU. Mechanical testing showed a transient phase during which properties increased. The delay of the composite in reaching adequate properties is strongly dependent on the energy dose. Conclusions: It is recommended that composites subject to unfavorable light curing conditions undergo a prolonged light curing process. (Journal of Applied Biomaterials & Biomechanics 2009; 7: 132-40)
  • 福岡杏理, 小城賢一, 井上哲, 吉田靖弘, 田中享, 池田考績, 鈴木一臣, 小松久憲, 佐野英彦
    日本歯科保存学雑誌 52 1 27 - 38 2009年02月 [査読有り][通常論文]
  • Futami Nagano, Denis Selimovic, Mamoru Noda, Takatsumi Ikeda, Toru Tanaka, Yusei Miyamoto, Ken-ichi Koshiro, Hidehiko Sano
    BIO-MEDICAL MATERIALS AND ENGINEERING 19 2-3 249 - 257 2009年 [査読有り][通常論文]
     
    Since adhesive technology was introduced into dental field, metal-based restoration has been gradually replaced by metal-free restoration. Using the adhesive technology, minimum invasive technique has been possible in daily clinical practice as well as esthetic tooth-colored restorations have become very popular all over the world. One of the current issues of the dental adhesive is durability of bond between tooth structure and adhesive resin. Several approaches to overcome the issues have been carried out. Self-etching approach is believed to create durable bond because demineralization of superficial tooth surface is very shallow. Other approach is to utilize the inhibitor of enzymes which are suggested to catalyze the decomposition of resin composites and are always secreted within the oral environment. In the present study, Colloidal Platinum Nanoparticles (CPN) was applied before the application of 4-META/MMA-TBB resin cement as the third possibility to prolong the durability of bond. This implies that the use of the CPN solution would create higher conversion at the interface compared with conventional bonding procedures.
  • 長野二三, 星加修平, 田中享, 佐野英彦
    北海道歯学雑誌 29 2 181 - 196 2008年12月 [査読有り][通常論文]
  • Andrea V. Kaneshiro, Satoshi Imazato, Shigeyuki Ebisu, Saori Tanaka, Yuko Tanaka, Hidehiko Sano
    DENTAL MATERIALS 24 10 1420 - 1427 2008年10月 [査読有り][通常論文]
     
    Objectives. The purpose of this study was to evaluate the ability of an experimental self-etching resin coating system to protect against demineralization of the root surface in vitro, comparing its effectiveness with two commercial one-step self-etching adhesives. Methods. The experimental resin coating system (EC), consisting of a self-etching primer and an acrylate-based resin, or the two commercial self-etching adhesives, Adper Prompt-L Pop (AD) and Clearfil Tri-S Bond (S3), was applied to the cementum of a human extracted tooth root. The specimens were immersed in acetate buffer at pH 5.5 for 4 weeks, and the degree of demineralization underneath the materials was evaluated by microscopic observation and contact microradiography. Each material's sealing and interfacial ultrastructure on the cementum was morphologically examined by scanning electron microscopy, and their bonding ability to cementum was determined by microtensile bond strength (MTBS) tests. Results. Application of AD and S3 showed limited effects to protect against demineralization, but no dernineralization was observed for EC. EC showed greater coating thickness than the other two materials, with formation of integral hybridized layers with cementum. Although the MTBS values for EC were significantly lower than those for AD and S3, cohesive failure in resins was the prevalent fracture mode for EC, showing no possible disadvantages in bonding ability. Significance. The experimental resin coating system effectively prevented acid demineralization on root surfaces due to its ability to produce an appropriately thick coating and an integral hybridized layer. (C) 2008 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • Takatsumi Ikeda, Jan De Munck, Kenichi Shirai, Kazuhiro Hikita, Satoshi Inoue, Hidehiko Sano, Paul Lambrechts, Bart Van Meerbeek
    DENTAL MATERIALS 24 10 1316 - 1323 2008年10月 [査読有り][通常論文]
     
    Objectives. The objectives of this study were (1) to clarify the relationship between the duration of air-drying of one-step self-etch adhesives (1-SEAs) and the evaporation degree (ED) of solvents, and (2) to evaluate the effect of ED on the ultimate micro-tensile strength (mu TS) of the adhesives. Methods. The ED of one HEMA-rich I-SEA, Clearfil S' Bond (Kuraray), and two HEMA-free 1-SEAs, iBond (Heraeus-Kulzer) and G-Bond (GC), was determined without air-drying and after, respectively, 5- and 10-s air-drying using a gravimetric method. Next, the mu TS of the adhesives at ED equivalent to 0-, 5- and 10-s air-drying was measured. Results. The ED increased with extension of air-drying time. Among the adhesives tested, iBond showed the largest ED, followed by G-Bond and Clearfil S-3 Bond in this order. A longer air-drying time for 10-s resulted in a statistically significantly higher mu TS for the HEMA-rich Clearfil S3 Bond. The mu TS of the latter was higher than that of the other two HEMA-free adhesives for each air-drying time. Significance. Air-drying of 1-SEAs had a significant effect on the degree of solvent evaporation (ED) and also on the mechanical properties (mu TS) of the 1-SEAs upon setting. It is therefore beneficial to remove solvents of the 1-SEAs as much as possible by thorough, strong air-drying in order to achieve a strong adhesive layer at the interface. (C) 2008 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • 川本千春, 福岡杏理, 星加修平, 田中享, 佐野英彦
    接着歯学 26 1 52 - 57 2008年04月 [査読有り][通常論文]
  • Papilla preservation technique combined with Emdogain in the treatment of intrabony defects: a novel treatment regimen for chronic periodontitis.
    Miliauskaite A, Selimovic D, Hassan M, Nagano F, Soell M, Sano H, Puriene A
    Stomatologija 10 1 22 - 26 2008年 [査読有り][通常論文]
  • 村田幸枝, 松田康裕, 田中享, 小松久憲, 佐野英彦
    日本歯科保存学雑誌 50 6 799 - 807 2007年12月 [査読有り][通常論文]
  • Fujisawa T, Yokoyama A, Muramatsu M, Kimura Y, Kurozumi A, Kobayashi I, Sano H, Totsuka Y, Fukushima K
    Special care in dentistry : official publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry 27 5 187 - 190 2007年09月 [査読有り][通常論文]
  • Takashi Nakata, Megumi Fujita, Futami Nagano, Mamoru Noda, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 26 5 635 - 641 2007年09月 [査読有り][通常論文]
     
    The purpose of this study was to examine the effect of a new thermal cycling method using PCR thermal cycler on the durability of two-step self-etching adhesive systems. Beam specimens for microtensile bond strength test were inserted into PCR tubes with distilled water. Thermal cycling (0, 1000, 2000, and 3000 cycles) was done with the PCR program at two different temperatures of 5 and 55 degrees C for 30 seconds each using a PCR thermal cycler. After thermal cycling, specimens were subjected to microtensile bond strength test and fractured surfaces were observed by SEM. The bond strength of Imperva Fluoro Bond after 3000 times of thermal cycling was significantly decreased compared to those of less than 2000 cycles (p<0.05), whereas SI-R20401 showed no significant differences even after 3000 cycles (p>0.05). Results of this study revealed that bond strength was affected by the thermal stress introduced in this experimental model. Therefore, this new thermal cycling method is a potentially useful and accessible means to evaluate the durability of dentin adhesive systems.
  • H. Komatsu, H. Yamamoto, M. Nomachi, K. Yasuda, Y. Matsuda, Y. Murata, T. Kijimura, H. Sano, T. Sakai, T. Kamiya
    NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS 260 1 201 - 206 2007年07月 [査読有り][通常論文]
     
    Using PIGE (TIARA, JAPAN) technique, we measured fluorine (F) uptake into the tooth enamel around a fluoride-containing material during caries progression using pH cycling. Class I cavities in the buccal surfaces of 6 extracted human teeth were drilled and filled with fluoride-containing material; a glass ionomer cement (Fuji IX(GC)). Three 300 mu m sections through the material were obtained from each tooth. Two of these specimens were utilized to measure the F distribution in enamel adjacent to the material. A 1.7 MeV proton beam accelerated by the TIARA single-ended accelerator was delivered to a micro-beam apparatus. The beam spot size was about 1 mu m with a beam current of about 100 pA. A nuclear reaction, F-19(p, alpha gamma)(16)0, was used to measure the F concentration and the gammarays from this reaction were detected with a 4" NaI detector. X-rays induced by proton were detected with a Si(Li) detector to measure calcium concentration and the beam intensity was monitored with the X-ray yield from a copper foil for quantitative analysis. After measurement of F uptake, all specimens were polished to a thickness of 120 mu m. In order to simulate daily acid challenges occurring in the oral cavity, the pH cycling (pH6.8-pH4.5) was carried out for 1, 3 and 5 weeks, separately. The duration that the solution remained below pH 5.5 was 37 min per cycle. The cycles were repeated 6 times per day with 2 h interval between cycles, and the specimens were kept in remineralizing solution for the rest of pH cycle. After pH cycling, F and calcium distribution of each specimen was evaluated using PIGE technique. The F distribution of the specimens before pH cycling clearly showed the F uptake from fluoride-containing material into enamel adjacent to the material. After pH cycling, the caries progression in all specimens was observed by the image of transverse micro radiography (TMR). The depth of caries and mineral loss progressed with increasing the duration of pH cycling, although the enamel adjacent to the material remained a caries inhibition zone due to low rate of demineralization. With caries progression, fluorine accumulated in the subsurface of the caries lesion, while the outermost surface of the caries lesion gradually dissolved under increasing pH cycling. The data obtained using PIGE (TIARA, JAPAN) technique were useful to understand the fluorine benefit for preventing dental caries by means of fluoride-containing dental materials. (c) 2007 Elsevier B.V. All rights reserved.
  • Yasuhiro Matsuda, Yukie Murata, Toru Tanaka, Hisanori Komatsu, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 26 3 414 - 421 2007年05月 [査読有り][通常論文]
     
    To the end of developing a convenient research tool to calculate the mineralization status of teeth in detail, a new program was developed using Visual Basic for Applications combined with Microsoft Excel 2004. To demonstrate the usefulness of this program, it was used to analyze tooth enamel mineralization after acid exposure. Transverse microradiography images (TMR) of specimens were digitalized with a charge-coupled device camera with a microscope (CCD camera) and a digital film scanner (FS). Subsequently, the mineral content profile of each specimen after de- and remineralization studies were calculated using the Angmar's formula. The newly developed program was applied to calculating the mineral loss (Delta Z), lesion depth (Ld), surface zone depth (SZd), and lesion body depth (LBd) of tooth specimens. In addition, the outer surface zone (OSZ), inner lesion body (ILB), and sandwich area (SA) between OSZ and ILB-which together constituted Delta Z-were calculated by the newly developed program. Data obtained with the newly developed program were in good agreement for both CCD camera and FS, indicating that the program was reliable for tooth enamel mineralization research studies.
  • S. K. Sidhu, Y. Omata, T. Tanaka, K. Koshiro, D. Spreafico, S. Semeraro, D. Mezzanzanica, H. Sano
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 80B 2 297 - 303 2007年02月 [査読有り][通常論文]
     
    This study evaluated the microtensile bond strength and the interfacial morphology of newer adhesives. The occlusal surfaces of extracted teeth were ground flat for random allocation to four equal groups. Resin composite was bonded to each surface using either Clearfil SE Bond [SEB], Clearfil Protect Bond [PB], G-Bond [GB], or an experimental adhesive, SSB-200 [SSB]. After storage for 24 h in water at 37 degrees C, they were sectioned into beams (cross-sectional area 1 mm(2)) for microtensile bond strength testing (mu TBS) at a crosshead speed of 1 mm/min. The load at failure of each was recorded; the data were analyzed by one-way ANOVA and Games Howell tests. The surfaces of the fractured specimens were observed using SEM. For the ultra-morphology of the interface, the occlusal surfaces of four more teeth were prepared as before and a thin layer of flowable resin composite was bonded to each surface using one of the four adhesives. The mean JXTBS ranged from 39.68 NlPa (GB) to 64.97 MPa (SEB). There were no statistical differences between SEB and SSB, or between PB and GB (p > 0.05). The mu TBS of SEB and SSB were significantly greater than that of PB and GB (p < 0.05). SEMs of the fractured surfaces revealed a mixed (cohesive/interfacial) failure. TEM examination highlighted differences in the hybrid layer; SEB had a thicker layer than the others. In conclusion, the newer all-in-one adhesives produced a thin hybrid layer but varied in their bond strengths. The 2-step self-etching adhesives do not necessarily produce higher bond strengths than that of the all-in-one systems. (c) 2006 Wiley Periodicals, Inc.
  • 垂直歯根破折の接着治療
    菅谷 勉, 田中佐織, 宮治裕史, 富田真仁, 長谷川有紀子, 川村直人, 田中裕子, 元木洋史, 中塚 愛, 川浪雅光, 加藤 熈, 佐野英彦, Sharanbir K. Sidhu
    日本歯科医学会誌 26 47 - 51 2007年 [査読無し][招待有り]
  • Saori Tanaka, Tsutomu Sugaya, Masamitsu Kawanami, Yoshinobu Nodasaka, Toshiki Yamamoto, Hiroshi Noguchi, Yuko Tanaka, Takatsumi Ikeda, Hidehiko Sano, Sharanbir K. Sidhu
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 80B 1 140 - 145 2007年01月 [査読有り][通常論文]
     
    Although 4-META/MMA-TBB resin has adhesive properties to dentin, and has been clinically used for the bonding treatment of vertically fractured roots and apicoectomy, there has not been any investigation on the adhesion of 4-META/MMA-TBB resin to cementum. The purpose of this in vitro study was to evaluate the bonding and the sealing ability of 4-META/MMA-TBB resin to cementum. Bovine root cementum and dentin surfaces were treated with a citric acid and ferric chloride solution, and the 4-META/MMA-TBB resin was applied on the treated surfaces before testing. The microtensile bond strength and the leakage levels obtained for the cementum were almost equal to those for the dentin. In SEM and TEM observations, a hybrid layer approximately 2-3 mu m in thickness was observed at the interface between the resin and the cementum. It is concluded that 4-META/MMA-TBB resin adhered to cementum via a hybrid layer on cementum, as previously reported for dentin. (c) 2006 Wiley Periodicals, Inc.
  • Jan De Munck, R. Banu Ermis, Kenichi Koshiro, Satoshi Inoue, Takatsumi Ikeda, Hidehiko Sano, Kirsten L. Van Landuyt, Bart Van Meerbeek
    JOURNAL OF DENTISTRY 35 1 74 - 83 2007年01月 [査読有り][通常論文]
     
    Objective: Phase-separation within HEMA-free all-in-one dental adhesives may result in the entrapment of droplets within the adhesive resin. Strongly air-blowing prior to polymerization, can remove most of these droplets. The objective of this study was to evaluate the effect these droplets may have on the resistance of the adhesive-tooth interface to NaOCl degradation. Methods: The micro-tensile bond strength (mu TBS) to enamel and dentin was determined when a HEMA-free all-in-one adhesive was applied either following a mild or strong air-blowing technique. The bonds were also exposed to an aqueous sodium hypochlorite (NaOCl) solution for 1 h, following a recently introduced methodology to mimic in vivo bond degradation. Results: This study revealed that strong air-blowing of the adhesive only resulted in a significantly higher micro-tensile bond strength (mu TBS) to dentin, but not to enamel. Likewise, NaOCl only reduced the mu TBS to dentin for both the mild and strong air-blowing technique, but again not the mu TBS to enamel. Failure analysis by SEM clearly revealed that strong air-blowing is less effective in droplet removal when the adhesive was applied in small and narrow class-I cavities, as compared to when it was applied to flat surfaces. Conclusions: NaOCl did preferentially dissolve the hybrid layer at dentin, and more for the mild than for the strong air-blowing technique. A strong air-blowing procedure resulted in a more NaOCl-resistant hybrid layer, so that it can be concluded that a HEMA-free one-step adhesive definitely benefits from a strong air-blowing technique. (C) 2006 Elsevier Ltd. All rights reserved.
  • Yasushi Shimada, Md Akhtar Uzzaman, Junji Tagami, Toru Tanaka, Takashi Nakata, Yasuko Nakaoki, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 26 1 122 - 128 2007年01月 [査読有り][通常論文]
     
    To compare the pulpal responses to light-cured and self-cured resin cements, cervical cavities were prepared in monkey's teeth, followed by application of etching gel and adhesive (Single Bond). A dual-cure resin cement (RelyX (TM) ARC) was applied, and hybrid composite inlays (Estenia) were bonded to the cavities. In one group, the cavities were photoirradiated for 20 seconds and the resin cement light-cured. In the other group, the resin cement was self-cured for six minutes without any photoirradiation. After experimental periods of seven, 28, and 70 days, histological features of pulp tissue were evaluated and compared. Results showed no significant differences in the histological features of the pulp tissues between the two curing methods. Both light-cured and self-cured resin cements showed acceptable biological compatibility with the monkey pulp. No bacterial penetration along the cavity walls was detected with either curing method.
  • Masanori Hashimoto, Franklin R. Tay, Hidehiko Sano, Masayuki Kaga, David H. Pashley
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 79B 2 453 - 458 2006年11月 [査読有り][通常論文]
     
    It is thought that water-filled channels and nanovoids in resin-dentin bonds represent potential sites for degradation of bonds or hydrolysis of collagen or both. How water gains access to bonded interfaces is not clear. This study evaluated the diffusion-induced water uptake through resin-dentin interfaces during bonding. Two light-cured total-etch adhesive systems (Excite and One-Step Plus) and a chemical-cured adhesive (Amalgambond Plus) were used in this study. Dentin disks were placed in a split-chamber device, and the fluid movement across dentin was measured, with and without a physiological pressure, during bonding procedures and 24 h after bonding. For light-cured adhesives in the experimental groups, a 6 min interval of dark storage was conducted prior to light-curing, to evaluate the diffusion of water through the uncured resin monomers, and to test the effect of prolonged infiltration time of adhesives on water permeability of bonds. Prolonged adhesive infiltration reduced the water permeability of resin-dentin bonds for light-cured adhesives. Water gradients produced by bonding systems contribute to water movement across the dentin-adhesive interfaces during bonding procedures. Differences in chemical formulations for adhesive systems may lead to differences in the extent of diffusion-induced water movement and the amount of water within the resin-dentin bonds. (c) 2006 Wiley Periodicals, Inc.
  • Yo Omata, Matt Folan, Melissa Shaw, Regina L. Messer, Petra E. Lockwood, David Hobbs, Serge Bouillaguet, Hidehiko Sano, Jill B. Lewis, John C. Wataha
    TOXICOLOGY IN VITRO 20 6 882 - 890 2006年09月 [査読有り][通常論文]
     
    Thioredoxin reductase (TrxR) reduces thioredoxin (Trx), thereby contributing to cellular redox balance, facilitating the synthesis of deoxy-ribose sugars for DNA synthesis, and regulating redox-sensitive gene expression. Auranofin is a gold compound that potently inhibits TrxR. This inhibition is one suspected mechanism of auranofin's therapeutic benefit in the treatment of rheumatoid arthritis. The use of other gold compounds to treat cancer or inflammatory disease may rely on their ability to inhibit TrxR. In the current study, we tested the hypothesis that a variety of gold compounds may inhibit TrxR. Methods: We exposed rat-TrxR1 to auranofin, gold sodium thiomalate, sodium aurothiosulfate, triphenyl phosphine gold chloride, or gold acetate, and measured TrxR activity ex vivo. We then compared TrxR1 inhibitory levels of gold compounds to those that inhibited mitochondrial activity of THP1 monocytes and OSC2 epithelial cells, estimated by succinate dehydrogenase activity. Results: All gold compounds inhibited TrxR1at concentrations ranging from 5 to 4000 nM (50% inhibitory concentration). The oxidation state of gold did not correlate with inhibitory potency, but ligand configuration was important. Au(I)-phosphine compounds (triphenyl phosphine gold chloride and auranofin) were the most potent inhibitors of TrxR. All TrxR1 inhibitory concentrations were sublethal to mitochondrial activity in both THP1 and OSC2 cells. Conclusions: Diverse types of gold compounds may be effective inhibitors of TrxR1 at concentrations that do not suppress cellular mitochondrial function. Inhibition may be optimized to some degree by altering the ligand configuration of the compounds. These results support future study of a variety of Au compounds for therapeutic development as inhibitors of TrxR L (c) 2006 Elsevier Ltd. All rights reserved.
  • S. K. Sidhu, T. Ikeda, Y. Omata, M. Fujita, H. Sano
    OPERATIVE DENTISTRY 31 5 598 - 603 2006年09月 [査読有り][通常論文]
     
    Objective. This study evaluated color and translucency changes caused by light curing resin composite materials. Methods. The CIEELAB parameters (L*, a* and b*) of disks of A2 and opaque A2 shades of Charisma (Heraeus-Kulzer), Solare (GQ and Filtek Supreme (3M) were evaluated on the backings of black, white and the material itself both before and after light curing to evaluate color and translucency changes (by means of calculating Delta E* and the translucency parameter, respectively). Results. Solare and Filtek Supreme showed significantly smaller color changes during light curing than Charisma; however, the value of Delta E* of all the products/shades was still in the clinically unacceptable range. Regarding translucency changes during light curing, the A2 and opaque A2 shades of Charisma showed a statistically significant increase, although no difference was observed in the other products. Conclusions. Solare and Filtek Supreme tended to show less changes in translucency and color during light curing compared to Charisma. Nevertheless, the changes in color during light curing were still in the range of unacceptable color change. Therefore, direct shade matching of these materials for a precise shade match should be performed by using the cured material.
  • M Hashimoto, FR Tay, NR Svizero, AJ de Gee, AJ Feilzer, H Sano, M Kaga, DH Pashley
    DENTAL MATERIALS 22 6 560 - 568 2006年06月 [査読有り][通常論文]
     
    Objectives: This study evaluated the effect of errors commonly made in using total-etch adhesives, on the resulting bond strength, fluid movement and nanoleakage of resin dentin bonds. Methods: Two total-etch adhesives were used for bonding to dentin according to the manufacturers' recommendations, with meticulous solvent evaporation (control), or with the introduction of common bonding errors-wet bonding without solvent evaporation (no evaporation), and dry bonding. Results: The 24-hour bond strength of the control was significantly higher than the other groups (p <0.05). For all groups, the higher initial permeability declined significantly after 24 h. The fluid movement across bonded dentin was similar in the control and dry bonding for both adhesives, whereas significantly higher permeability (p<0.05) was recorded for the no evaporation groups even after 24 h. Extensive silver impregnation within hybrid layers was seen by TEM in the no evaporation and dry bonding specimens after 24 h. Dry bonding caused collapse of the collagen matrix and interfered with resin infiltration. In contrast, inadequate solvent evaporation and/or residual water during dentin bonding results in dilution or incomplete polymerization of the resin, leading to severe nanoleakage formation. Significance: Increased permeability associated with incomplete solvent evaporation in total-etch adhesives may lead to poor bond strength. (C) 2005 Published by Elsevier Ltd on behalf of Academy of Dental Materials. All rights reserved.
  • Yasuhiro Matsuda, Hisanori Komatsu, Yukie Murata, Toru Tanaka, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 25 2 280 - 285 2006年06月 [査読有り][通常論文]
     
    The aim of this study was to develop and evaluate an automatic pH-cycling system, which was intended for performing time-lapse analyses of dental caries. Two single-section specimens prepared from each of the five extracted human incisors were studied under a condition of 3- or 9-cycles/day for seven weeks. Increase of mineral loss (Delta Delta Z) and increase of Ld (Delta Ld) were examined and compared at the end of every week-during the study. In the 3-cycles/day condition, Delta Delta Z and Delta Ld significantly increased only at Week 7. In the 9-cycles/day condition, Delta Delta Z increased significantly from Week 5. Delta Ld increased from Week 2, and then there were no further increases for the subsequent five weeks. Based on the results of this study, progression pattern was found to be significantly different between the two conditions. We therefore concluded that the automatic pH-cycling system which we have developed qualified as a model system that simulated the human oral cavity. As such, it could be beneficially employed to clarify the roles of many factors that cause or prevent caries development in human teeth.
  • Megumi Fujita, Susumu Kawakami, Mamoru Noda, Hidehiko Sano
    DENTAL MATERIALS JOURNAL 25 2 352 - 359 2006年06月 [査読有り][通常論文]
     
    Recently, an esthetic tooth coating material has been developed. The material consisted of a primer solution, a base coat, and a top coat. The purpose of this study, therefore, was to evaluate the color change of this tooth coating material and two resin composites after immersion in food-simulating, staining solutions. To this end, the newly developed coating material with and without its top coat, a flowable resin composite, and a hybrid resin composite were employed for the evaluation. The specimens were subjected to an experimental 24-hour staining cycle: 7-hour immersion in coffee, green tea or red wine, then 17-hour immersion in artificial saliva solution containing 0.3% mucin. After 24 hours, 3 days, 1, 2, and 4 weeks of immersion, the color changes of all specimen surfaces were measured.. Compared with the other materials, the Delta E* value of coating material without its top coat tended to increase as the immersion period increased until 4 weeks. On the other hand, the Delta E* value of coating material with its top coat measured the lowest among the materials tested. Based on the results obtained, it was concluded that when using this recently developed tooth coating material in dental clinics, its top coat should be properly applied.
  • K Koshiro, SK Sidhu, S Inoue, T Ikeda, H Sano
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 77B 2 401 - 408 2006年05月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the morphological appearance of resin-dentin interface produced by 1-step adhesive systems in order to classify them, using TEM. It was hypothesized that all interfaces produced by each 1-step adhesive show characteristics similar to that produced by the conventional self-etching system rather than that produced by etch-and-rinse system. The flattened dentin surfaces were subjected to bonding treatment using either a 2-step adhesive: single bond, mega bond, unifil bond; or a 1-step adhesive: Prompt L-Pop, absolute, xeno CF, AQ bond, one-up bond F, G bond, and SSB-200. A flowable resin was then applied to these surfaces. The results revealed that resin-dentin interfaces produced by 1-step systems were classified into three categories. Additionally, the interface produced by G bond and SSB-200 showed a different morphological appearance from that of the others. The interface produced by these adhesives was thinner and less demineralized, with the presence of hydroxyapatite-coated collagen fibrils. It is proposed that "Nanointeraction Zone (NIZ)" is a new complementary classification for partially demineralized resin-dentin interdiffusion zones. Thus, one-step self-etching adhesive systems can be classified into three categories, etch-and-rinse type, mild self-etching type, and NIZ type, by their morphological appearance of the interface. (c) 2006 Wiley Periodicals, Inc.
  • S Semeraro, D Mezzanzanica, D Spreafico, M Gagliani, D Re, T Tanaka, S KSidhu, H Sano
    OPERATIVE DENTISTRY 31 3 317 - 323 2006年05月 [査読有り][通常論文]
     
    This study compared the microtensile bond strength (MTBS) of three all-in-one adhesive systems and a two-step system using two types of burs to prepare the dentin surfaces. Flat coronal surfaces of 24 extracted human molars were produced using either regular-grit or superfine-grit diamond burs. Resin composite was then bonded to equal numbers of these surfaces using one of the four adhesives: Clearfil SE Bond (CSE), G-Bond (GB), SSB-200 (SSB) or Prompt L-Pop (PLP). After storage for 24 hours in 37 C distilled water, the bonded teeth were sectioned into slices (0.7-mm thick) perpendicular to the bonded surface. The specimens were then subjected to microtensile testing and the bond strengths were calculated at failure. Bond strength data were analyzed by two-way ANOVA and the Games-Howell test for interaction between adhesive and type of cut dentin. The fractured surfaces were observed by SEM to determine the failure mode. In addition, to observe the effect of conditioning, equal numbers of the two bur-cut dentin surfaces of eight additional teeth were conditioned with the adhesives and observed by SEM. Based on the results, when CSE and SSB were bonded to dentin cut with a regular-grit diamond bur, the MTBS values were significantly lower than that of superfine bur-cut dentin; whereas, GB and PLP showed no significant differences in MTBS between the two differently cut surfaces. SEM observation of the fractured surfaces revealed a mixed mode (adhesive in some areas and cohesive in others in the same sample) of failure in all specimens except PLP, which showed cohesive failure within the adhesive for both types of bur preparation. Generally, SEMs of the conditioned surfaces using both types of burs showed partial removal of the smear layer for CSE, minimal for GB and SSB and complete removal for PLP. In conclusion, when cutting dentin, selecting the proper bur type is important for improving the bond strength of some self-etching adhesive systems.
  • Fluoride release and uptake by various dental materials after fluoride application
    K Okuyama, Y Murata, PNR Pereira, PA Miguez, H Komatsu, H Sano
    AMERICAN JOURNAL OF DENTISTRY 19 2 123 - 127 2006年04月 [査読有り][通常論文]
     
    Purpose : To measure the amounts of fluoride released from fluoride-containing materials before and after daily topical fluoride applications. Methods: A conventional glass-ionomer: Fuji lonomer Type II (F2); a resin-modified glass-ionomer: Fuji lonomer Type II LC (LC); two "giomer" materials: Reactmer Paste (RP), and Beautifil (BT); a fluoride-containing resin composite: Unifil F (UF); and a non-fluoride resin composite: AP-X (AP) were used in this study. Each material was filled into a plastic mold, with inner diameter of 9 mm wide x 3 mm high. The specimens were stored in vials filled with 8 ml distilled deionized water for 24 hours at 37 degrees C. The specimens were then removed from the vials and the amount of fluoride released into the water, over the 24-hour period, was measured. The amount of fluoride released was measured by using specific fluoride electrode and an ion-analyzer. These procedures were repeated at Days 2, 3, 7, 14, and 21. After 21 days, all specimens were exposed to 1000 ppm F NaF solution for 5 minutes once a day. This procedure and measurement of fluoride release were continued for 14 days. After 14 days, the specimens were placed in water for 7 days and fluoride release was measured. The results were statistically analyzed using Kruskal-Wallis and Mann-Whitney U-test (P< 0.05). Results: At the 22nd day (1 day after starting fluoride exposure), there was no difference between the F2 and R-P, though there were significant differences between the two GICs and the groups BT and UF. After that day, there were significant differences between GIC and the group RP, BT and UF. All materials showed a decrease in fluoride release 7 days after end of the fluoride immersion period. F2, LC, and UF showed no significant difference of fluoride release between Day 21 and I day after the end of the fluoride immersion period (P= 0.310: F2 and UF, 0.548: LC). On the other hand, RP and BT revealed lower fluoride release I day after the end of the fluoride immersion period as compared to Day 21 (P= 0.075: RP, 1.000: BT). For AP, fluoride release was not detected after the fluoride immersion period.
  • D Spreafico, S Semeraro, D Mezzanzanica, D Re, M Gagliani, T Tanaka, H Sano, SK Sidhu
    JOURNAL OF DENTISTRY 34 3 237 - 244 2006年03月 [査読有り][通常論文]
     
    Purpose: To evaluate the technique sensitivity of four different adhesive systems using different air-blowing pressure. Methods: Four adhesive systems were employed: Clearfil SE Bond [SE] (Kuraray, Japan), G-Bond [GB] (GC Corporation, Japan), Adper Prompt L-Pop [LP] (3M ESPE, USA) and an experimental adhesive, SSB-200 [SSB] (Kuraray, Japan). Twenty-four extracted molars were used. After grinding the coronal enamel surface, the teeth were divided into two equal groups. The first group's teeth were randomly assigned for bonding with the different adhesives using gentle air-blowing (g). For the teeth of the second group, the four adhesive systems were applied using strong air-blowing (s). After storage overnight in 37 degrees C water, the bonded specimens were sectioned into sticks (1 mmX 1 mm wide), which were subjected to microtensile bond strength testing (mu TBS) at a crosshead speed of 1 mm/min. The load at failure of each specimen was recorded and the data were analyzed by one-way ANOVA and Tukey HSD tests. The surfaces of the fractured specimens were observed using SEM to determine the failure mode. Results: The results of the pTBS test showed that the highest bond strengths tended to be with SE for both gentle and strong air-blowing, and the significantly lowest for SSB with strong air streaming. Comparing the two techniques, significant differences were noted only for SSB-200 (P<0.05). For each material, the SEM evaluation did not show distinct differences in the nature of the fractures between the two techniques, except for SSB-200. Conclusions: The adhesives tested are not technique sensitive, except SSB-200, with regards to the air-blowing step. (C) 2005 Elsevier Ltd. All rights reserved.
  • N Kamishima, T Ikeda, H Sano
    DENTAL MATERIALS JOURNAL 25 1 26 - 31 2006年03月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the effect of the covering enamel layer of various thicknesses on the color of layered resin composites (LRC). To this end, the CIELAB parameters of the following were evaluated: underlying base (ULB: 2.0 mm of body shade + 3.0 mm of opaque shade), inherent color of the enamel shade (4.0 mm of enamel shade + ULB), and LRC disks (0.5, 1.0, 2.0, 3.0 mm of enamel shade + ULB) of Filtek Supreme (FS, 3M) and Gradia Direct (GD, GC). To assess the effect of the enamel layer on the color of LRC, color difference between ULB and LRC was calculated. With FS, the enamel layer had no major effect on the color of LRC - regardless of thickness. With GD, the enamel layer had a significant effect on the color of LRC, even if the thickness was only 0.5 mm. The difference in enamel layer effect between the two products could be attributed to the difference in color characteristics of the two enamel shades.
  • Y Omata, S Uno, Y Nakaoki, T Tanaka, H Sano, S Yoshida, SK Sidhu
    DENTAL MATERIALS JOURNAL 25 1 125 - 131 2006年03月 [査読有り][通常論文]
     
    This study examined the surface staining mechanism of a photopolymerized composite by coffee, oolong tea, and red wine. Dental composite was subjected to an experimental 24-hour staining cycle: 17-hour immersion in artificial saliva solution containing 0.3% mucin followed by 7-hour immersion in coffee, tea, or wine. After one, two, and four weeks, digital images of the composite surface were analyzed in grayscale mode with an imaging analyzer. Specimens polished but not immersed were used as a baseline measurement for color change. Additionally, the effects of mechanical brushing and chlorhexidine on drink-induced staining were examined. Wine caused the most severe staining, followed by tea and coffee. After four weeks of immersion, brushing reduced surface staining by wine. On the contrary, chlorhexidine increased the staining effect of tea and coffee (p<0.05) when compared to the control specimens. In conclusion, we showed that common drinks stained the dental composite, but each by a specific mechanism that depended on external conditions such as the presence of chlorhexidine.
  • K Okuyama, T Nakata, PNR Pereira, C Kawamoto, H Homatsu, H Sano
    OPERATIVE DENTISTRY 31 1 135 - 142 2006年01月 [査読有り][通常論文]
     
    This study investigated the effect of fluoride containing resin composites and bonding agents, as well as the topical fluoride (F) application on the inhibition of artificial caries progression by using a pH-cycling model with alternating demineralizing (pH:4.5) and remineralizing (pH:7.0) solutions. Two bonding systems (F-containing bonding system [Reactmer Bond: RB] and non-F containing bonding system [Clearfil SE Bond: SE]), two resin composites, (F-containing [Reactmer Paste: RP] and non-F containing [Clearfil AP-X: AP]) were used. A combination of each bonding agent and a resin composite, RB+RP, RB+AP, SE+RP and SE+AP, was placed in 2 x 3 x 1.5-mm cavities on root dentin of extracted molars (n=96). Specimens were subjected to pH-cycling for 6 or 12 weeks. Half of all specimens were immersed in 0.05% NaF solution for 1 minute once a day as a topical F application. After the pH cycling period, a microradiograph of each specimen was taken, and the outer lesion depth of the artificial caries was measured by means of image analyzing software. The depths of the outer lesions at different periods were analyzed by one-way ANOVA and Sheffe's test at p=0.05. The combination that received F treatment showed reduced lesion depth compared to the same combination without F application. Except for the F application group of 12 weeks, there was no significant difference in lesion depth among each bonding and composite combination (p>0.05). At week 12 with the F application, RB+RP showed the shallowest lesion compared to the other combinations (p<0.05). The results indicated that the F application reduced the progression of artificial caries. Moreover, the combination of fluoride containing bonding agent and restorative material was the most effective for the inhibition of artificial caries progression based on the 12-week experimental period with topical F application.
  • 松田康裕, 小松久憲, 村田幸枝, 田中享, 佐野英彦
    日本歯科保存学雑誌 48 6 828 - 834 2005年12月 [査読有り][通常論文]
  • W Sasakawa, Y Nakaoki, F Nagano, S Horiuchi, T Ikeda, T Tanaka, M Noda, S Inoue, H Sano, SK Sidhu
    DENTAL MATERIALS JOURNAL 24 4 617 - 627 2005年12月 [査読有り][通常論文]
     
    The micro-shear bond strengths (MSBSs) of five single-step self-etch adhesives (Adper Prompt L-Pop [APL], AQ Bond plus [AQP], OBF-2 [OB2], Reactmer Bond [RB], and Xeno III [XIII]) were compared with that of a two-step self-etch adhesive, Clearfil SE Bond [SE]. The adhesives were applied on dentin surfaces, according to manufacturers' instructions, for bonding of resin composite to dentin. After 24 hours, a micro-shear bond test was carried out and the data were analyzed by oneway ANOVA and Bonferroni test (p < 0.05). The mean MSBSs in MPa were APL: 22.8, AQP: 37.4, 0132: 34.7, RB: 28.3, XIII: 30.3, and SE: 47.2. Among the single-step adhesives, the MSBS of AQP and OB2 were significantly higher than APL (p < 0.05). In conclusion, the single-step adhesives used in this Study, apart from AQP, produced significantly lower MSBS than the two-step adhesive (p < 0.05).
  • T Ikeda, J De Munck, K Shirai, K Hikita, S Inoue, H Sano, P Lambrechts, B Van Meerbeek
    DENTAL MATERIALS 21 11 1051 - 1058 2005年11月 [査読有り][通常論文]
     
    Objective. The purpose of the present study was to evaluate the effect of evaporation of primer components on the ultimate tensile strength (UTS) of primer-adhesive mixtures. Methods. The UTSs of 1:3 by weight primer-adhesive mixtures and of the pure adhesives (controls) were measured after 0-, 2-, 5-min and 24-h storage in a 37 degrees C oven for the three-step etchftrinse adhesive OptiBond FL (Kerr) and the two-step self-etch adhesive Ctearfil SE (Kuraray). Results. Evaporation of primer components increased with time for both adhesives investigated. OptiBond FL showed a higher evaporation of primer components than Ctearfil SE at the 2-, 5-min and 24-h storage time. As compared to the pure adhesive, the UTS of the primer-adhesive mixture was lower after 0- and 2-min storage for OptiBond FL and after 0-, 2- and 5-min storage for Clearfil SE. The UTS of OptiBond FL was higher than that of Clearfil SE at the 0- and 2-min storage time, though there was no difference at the 5-min and 24-h storage time. Significance. The degree of evaporation of primer components depended largely on the primer solvents employed. The significant decrease in UTS of adhesive resin when mixed with primer must be attributed to incomplete evaporation of primer components. Complete evaporation of solvents is hard to achieve, even by thorough air drying. (c) 2005 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • Y Nakaoki, W Sasakawa, S Horiuchi, F Nagano, T Ikeda, T Tanaka, S Inoue, S Uno, H Sano, SK Sidhu
    JOURNAL OF DENTISTRY 33 9 765 - 772 2005年10月 [査読有り][通常論文]
     
    Objectives: To evaluate the effect of double-application of all-in-one adhesives using the micro-shear bond test. Methods: The occlusal surfaces of extracted human third molars were ground perpendicular to the long axis of each tooth to expose a flat dentin surface. Three commercially available and one experimental bonding system were used in this study: Adper Prompt L-Pop (APL, 3M ESPE), REACTMER BOND (RB, Shofu), XENO III (Xeno, Dentsply-Sankin) and newly developed OBF-2 (0132, Tokuyama Dental). These adhesives were applied on the dentin surfaces by either the manufacturers' instructions or by an experimental method (single-application or double-application). Resin composite was then placed and light-cured for 40 s. After 24 h immersion in water, a micro-shear bond test was carried out and the fractured dentin surfaces were observed microscopically. The data were analyzed by one-way ANOVA and Tukey's HSD tests (p < 0.05). Results: The mean bond strengths of APL, RB, Xeno and OB2 with single-application were 22.7, 28.3, 30.3 and 34.6 MPa, respectively. The mean bond strengths of APL, RB, Xeno and OB2 with double-application were 29.5, 27.2, 29.6 and 32.5 MPa, respectively. There were no statistically significant differences in micro-shear bond strengths between the single- and double-application methods for each adhesive system (p > 0.05). The morphological observation of the fractured dentin surfaces revealed differences between the single- and double-application groups especially for APL and OB2. Conclusions: Micro-shear bond strengths of all-in-one adhesives in this study showed no statistically significant differences between the single-application and the double-application method. (c) 2005 Elsevier Ltd. All rights reserved.
  • N Kamishima, T Ikeda, H Sano
    DENTAL MATERIALS JOURNAL 24 3 428 - 432 2005年09月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the colors of resin composites used for the layering technique, as well as the translucency at various thickness. For the purpose of calculating the translucency parameter (TP), black and white backings were used to evaluate the CIELAB parameters of Filtek Supreme (3M) and Gradia Direct (GC) disks of various thicknesses (0.5, 1.0, 2.0, 3.0 and 4.0 mm) and of three different shades (enamel-shade, body-shade, and opaque-shade). As for the color of each shade, it was evaluated using 4mm thick specimens on the white backing. Regarding TP, the opaque-shades were less translucent than the other shades. It was also found that translucency increased exponentially as thickness was reduced - regardless of shade. Regarding color, enamel-shades were more bluish (especially in GD) compared to the other shades, and the opaque-shades displayed a brighter and yellowish characteristic. In the layering technique, it is fundamental to have an accurate knowledge about the translucency and colors of the materials/shades used.
  • M Hashimoto, FR Tay, S Ito, H Sano, M Kaga, DH Pashley
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 74B 2 699 - 705 2005年08月 [査読有り][通常論文]
     
    The purpose of this study was to quantify the permeability of adhesive resin films to water by measuring convective flow across thin films. Cured resin films were prepared with the use of five commercially available adhesive resins and an experimental resin. Two types of resin films were prepared from solvated comonomer blends within each product; resin films made before or after evaporation of solvent (10 s of drying with air-syringe). The permeability of the resin films was measured 30 min or 24 h after polymerization by placing the films in a split-chamber device. Fluid filtration rate through the resin films was measured with the use of 20 cm of water pressure. Osmotically induced water movement was measured by applying hypertonic aqueous solutions of CaCl2 or HEMA to the resin films. The results showed that evaporation of solvent before polymerization reduced the permeability of adhesive resin films, compared to permeabilities obtained without solvent evaporation. The cured adhesive resin films were all permeable to water, but to varying degrees. (c) 2005 Wiley Periodicals, Inc.
  • K Koshiro, S Inoue, H Sano, J De Munck, B Van Meerbeek
    EUROPEAN JOURNAL OF ORAL SCIENCES 113 4 341 - 348 2005年08月 [査読有り][通常論文]
     
    Resin-dentin bonds are known to degrade in the relatively aggressive oral environment. In order to obtain greater insight into the interfacial degradation process, we examined, by using transmission electron microscopy (TEM), the interfacial ultrastructure of two adhesives bonded to dentin after 1 yr in vivo. Class V cavities were prepared on the buccal surfaces of 14 intact teeth of two monkeys and then restored by using either the two-step self-etch adhesive, Unifil Bond, or the two-step etch-and-rinse adhesive, Single Bond, in combination with the restorative microhybrid composite, Z250. After 1 yr, 10 other teeth were restored by using the same materials (controls). One day later, the monkeys were killed, following which the microtensile bond strength to dentin was determined and the interfacial ultrastructure was examined by TEM. Whereas no noticeable changes in the morphology of the resin-dentin interface were observed between the 1-d and 1-yr specimens for Unifil Bond, Single Bond exhibited signs of interfacial degradation, in particular at the bottom of the 3 mu m-deep hybrid layer. In conclusion, the adhesive interface produced by the etch-and-rinse adhesive was less resistant to degradation than that produced by the self-etch system.
  • M Noda, JC Wataha, JB Lewis, M Kaga, PE Lockwood, RLW Messer, H Sano
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 73B 2 308 - 314 2005年05月 [査読有り][通常論文]
     
    The use of hydrophilic dental monomers in dentin bonding agents has vastly improved resin-dentin bond strengths, but incomplete polymerization of these monomers and their leaching into adjacent (pulpal) oral tissues has raised concerns about their biocompatibility. The sublethal effects of these resins are virtually unknown, but their electrophilic nature led to the hypothesis that they may alter cellular oxidative stress pathways. Glutathione balance between reduced (GSH) and oxidized (GSSG) is a major mechanism by which cells maintain redox balance and was therefore the focus of the current investigation. THP-1 human monocytic cells were exposed to hydroxyethyl methacrylate (HEMA), benzoyl peroxide (BPO), camphorquinone (CQ), or triethyelene glycol dimethacrylate (TEGDMA) for 24 h at sublethal doses, then GSH and GSSG levels were measured by means of Ellman's method adapted for cell culture. The results indicate that these dental resin compounds act at least partly via oxidative stress by increasing GSH levels at sublethal concentrations. However, the GSH-GSSG ratio was relatively unaffected. Only BPO altered the GSH-GSSG ratio at 24 h, again at sublethal levels (7.5-15 μ mol/L). The results support the hypothesis that resin monomers act, at least in part, via oxidative stress, and that oxidative-stress pathways should be one focus of future investigations of monomer biocompatibility. © 2005 Wiley Periodicals, Inc.
  • T Ikeda, J De Munck, K Shirai, K Hikita, S Inoue, H Sano, P Lambrechts, B Van Meerbeek
    DENTAL MATERIALS 21 5 413 - 420 2005年05月 [査読有り][通常論文]
     
    Objective. The purpose of this study was to measure the micro-tensile bond strength (JBS) of two adhesives to dentin and to correlate this JBS with the micro-tensile fracture strength (mu TFS) of primer-adhesive mixtures of the two adhesives. Methods. The three-step etchEtrinse adhesive OptiBond FL (Kerr) and the two-step self-etch adhesive Clearfil SE Bond (Kuraray) were used to bond Filtek Z100 (3M ESPE) to twenty mid-coronal dentin surfaces. The mu TBS was determined at 1 and 24 h after tight-curing. In addition, the mu TFS of primer-adhesive mixtures (primer: adhesive= 1:3 by weight) and of the pure adhesives (controls) were measured after the same time intervals. Results. The JBS to dentin of OptiBond FL at 24 h was significantly higher than at 1 h and than the mu TBS of Clearfil SE Bond at 24 h. The mean mu TFS of the primeradhesive mixtures was always lower than the mu TFS of the respective adhesives. The highest reduction in mu TFS due to mixing primer with the adhesive resin was measured for Clearfil. SE Bond. Both the mu TFS of the primer-adhesive mixtures and the pure adhesives of OptiBond FL and Clearfil SE Bond increased with time. Significance. The early 1 h mu TBS to dentin was lower than the 24 h mu TFS for OptiBond FL, but not for Clearfil SE Bond. Differences in polymerization efficiency as well as in the resulting interfacial strength may explain such varying early bond strengths to dentin. (c) 2004 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • T Ikeda, SK Sidhu, Y Omata, M Fujita, H Sano
    EUROPEAN JOURNAL OF ORAL SCIENCES 113 2 170 - 173 2005年04月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the optical properties - not only the translucency but also the colours - of opaque-shade resin composites. The CIELAB parameters (L*, a* and b*) of disks of A2 and opaque A2 (OA2) shades of Charisma (Heraeus-Kulzer), Solare (GC) and Filtek Supreme (3M) were evaluated on backings of black, white and the material itself to calculate the translucency parameter (TP) and the colour differences (Delta E*) between A2 and OA2. A two-way analysis of variance (ANOVA) for the TP indicated a less statistically significant TP value in the OA2 shade than the A2 shade for all products. As for the products, Charisma showed a statistically greater TP value than the other two products. Regarding the Delta E* between A2 and OA2, all the products revealed clinically perceptible colour differences (Delta E* > 3.3). Hence, we must take the colour differences of opaque-shade resin composites into consideration, as well as the translucency of the materials, for a clinically acceptable colour match of the restoration.
  • Bond strength and SEM observation of CO2 laser irradiated dentin, bonded with simplified-step adhesives
    K Koshiro, S Inoue, K Niimi, K Koase, H Sano
    OPERATIVE DENTISTRY 30 2 170 - 179 2005年03月 [査読有り][通常論文]
     
    This study investigated, mechanically and morphologically, whether the dentin surface irradiated by CO2 laser could be a possible adherent when bonded with simplified-step adhesives. Buccal enamel and cementum of extracted human premolars were removed to expose a flat dentin surface. The dentin surfaces were irradiated continuously with CO2 laser at 1.0 W. Before bonding with either a single-bottle adhesive (Single Bond) or a self-etching priming system (Mega Bond), the irradiated dentin surface was treated as follows: no treatment, NaHCO3 powder abrasion and wet-grinding with 600-grit SiC paper. The treated dentin surfaces were bonded to resin composite with either of the two adhesives. Non-irradiated dentin surfaces were also used as control. Resin bonded specimens were stored in water at 37 degrees C for 24 hours and subjected to microtensile bond test. Additionally, to observe the resin/irradiated dentin interface, resin-bonded specimens were similarly prepared, sectioned into slabs, embedded in epoxy resin, polished with diamond pastes, sputter coated Au-Pd and examined with scanning electron microscopy (SEM). After SEM observation, the specimens were further polished with diamond paste to remove the Au-Pd sputter-coat, immersed in HCL and NaOCl and finally observed by SEM again. In the presence of carbonized dentin, microtensile bond strength drastically decreased but recovered to the control value by removing the carbonized dentin layer visually with SiC paper for both adhesive systems. However, the laser-affected dentin that remained on the bonded interface was easily dissolved with NaOCl and HCl.
  • M Fujita, S Kawakami, H Komatsu, H Sano
    DENTAL MATERIALS JOURNAL 24 1 111 - 116 2005年03月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the translucency and color change of simulated heavily discolored teeth using polymer-based dental tooth coating materials and flowable resin composites. Five shades of coating material and two shades of flowable resin composite were used. Colorimetric values of the materials in different shades were determined using the L*a*b* system of the Commission Internationale de I'Eclairage (CIE). Colorimetric examination was performed on white, black, and shade guide (C4) backgrounds using specimens of various thickness (0.2, 0.3, 0.5, 1.0, and 2.0 mm). New coating material showed less translucent than flowable resin composites. Moreover the new material showed the potential to improve the appearance of heavily discolored teeth when being applied as a thin first layer.
  • Inoue S, Koshiro K, Yoshida Y, De Munck J, Nagakane K, Suzuki K, Sano H, Van Meerbeek B
    J Dent Res 2005年 [査読無し][通常論文]
  • Hashimoto M, Ito S, Tay FR, Svizero NR, Sano H, Kaga M, Pashley DH
    Journal of dental research 83 11 843 - 848 2004年11月 [査読有り][通常論文]
  • Effect of conditioner on bond strength of glass-ionomer adhesive to dentin/enamel with and without smear layer interposition
    S Inoue, Y Abe, Y Yoshida, J De Munck, H Sano, K Suzuki, P Lambrechts, B Van Meerbeek
    OPERATIVE DENTISTRY 29 6 685 - 692 2004年11月 [査読有り][通常論文]
     
    The effect of a polyalkenoic acid conditioner pretreatment on the bond strength of a glass-ionomer adhesive to tooth substrates with or without smear layer was evaluated. Smear-layer covered and smear-layer free dentin and enamel surfaces were prepared from 24 extracted human molars. Resin composite was bonded to the sur faces using FuJiBond LC with or without a polyalkenoic acid conditioner and subjected to microtensile bond strength (muTBS) testing. Failure modes were determined using scanning electron microscopy. For dentin, smear-layer coverage and conditioner treatment did not reveal significant differences in muTBS, which ranged from 20 to 29 MPa. For enamel, smear-layer coverage did not significantly affect muTBS, whereas, the use of conditioner significantly improved MTBS, reaching the same MTBS-values as when bonded to dentin. Regarding failure mode, most dentin specimens failed mixed adhesive cohesively. For enamel, adhesive failures mostly occurred when no conditioner was used, though mixed failures were predominant when the specimens were conditioned beforehand. Bonding of the glass ionomer adhesive to dentin can be achieved without the separate use of a polyalkenoic acid conditioner, even with the interposition of a smear layer. However, instrumented and non-instrumented enamel requires separate conditioning to provide sufficient micro-mechanical retention.
  • A Takahashi, F Higashino, M Aoyagi, S Kyo, T Nakata, M Noda, M Shindoh, T Kohgo, H Sano
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 71B 1 214 - 221 2004年10月 [査読有り][通常論文]
     
    Bisphenol A (BPA) is one of the endocrine-disrupting chemicals (EDCs) that possess estrogen-like biologic activity. Many dental materials have been reported to release BPA. However, there are few reports available on the release of BPA from dental polycarbonates. The purpose of this study was to investigate the release of BPA from dental pollycarbonate crowns and to evaluate the estrogenic activity of BPA. Pollycarbonate crowns were immersed in five solvents (water, ethanol, n-hepthane, acetic acid, and acetonitrile) at 37 or 65 degreesC for 24 h. The elution from the material was analyzed by high-performance liquid-chromatography (HPLC) and mass-spectrometry (MS) analysis. BPA release was detected corresponding to the degradation of dental polycarbonates under the some storage conditions (ethanol, acetic acid, and acetonitrile). A previous report proved that estrogen increased human telomerase catalytic subunit (hTERT) mRNA, whereas the effect of EDCs on the hTERT promoter has never been reported. The estrogenic activity of BPA was analyzed by luciferase assay with the use of the hTERT promoter. This assay revealed that BPA was a positive regulator of hTERT transcription. In addition, quantitative real-time PCR analysis showed that BPA increased the expression level of hTERT mRNA in MCF7 cells. Herein, it is demonstrated that hTERT is a new target of BPA. (C) 2004 Wiley Periodicals, Inc.
  • Takahashi A, Higashino F, Aoyagi M, Kyo S, Nakata T, Noda M, Shindoh M, Kohgo T, Sano H
    Journal of biomedical materials research. Part B, Applied biomaterials 71 1 214 - 221 2004年10月 [査読有り][通常論文]
  • Effects of multiple adhesive coatings on dentin bonding
    M Hashimoto, H Sano, E Yoshida, M Hori, M Kaga, H Oguchi, DH Pashley
    OPERATIVE DENTISTRY 29 4 416 - 423 2004年07月 [査読有り][通常論文]
     
    Simple changes to bonding techniques can improve resin-dentin bond strengths. This study evaluated the effect of multiple consecutive coatings of adhesive resin on dentin by measuring both microtensile bond strength and nanoleakage following exposure to ammoniacal silver nitrate. Resin-dentin bonded specimens were prepared using two total-etch adhesives (OptiBond Solo Plus/Kerr or Single Bond/3M ESPE). During bonding, resin application and air evaporation were done 1, 2, 3, 4, 6 or 8 times on acid-etched, moist dentin surfaces. Mean microtensile bond strengths were evaluated by two-way ANOVA and Fisher's PLSD test (p<0.05; n=16 for each group). Additionally, nanoleakage of silver nitrate was evaluated by transmission electron microscopy (TEM). The results indicated that bond strengths increased with each coating up to four coats. Nanoleakage decreased with each coat, becoming very small after four or more coats. This adhesive application method can be easily applied to clinical practice, thereby improving the quality of resin-dentin bonds.
  • In vitro testing of all-in-one adhesives as fissure sealants
    M Hori, E Yoshida, M Hashimoto, M Kaga, H Sano, H Oguchi
    AMERICAN JOURNAL OF DENTISTRY 17 3 177 - 181 2004年06月 [査読有り][通常論文]
     
    Purpose: To evaluate the clinical efficacy of all-in-one adhesives as fissure sealants. Methods: Resin-enamel bonded specimens were fabricated between polished and ground enamel surfaces using two all-in-one adhesives (One-Up Bond F and Xeno CF Bond) and two resin sealants (Teethmate F-I and Helioseal F). Specimen beams were prepared and microtensile bond tests were performed at a crosshead speed of 1 mm/minute. The bond strengths obtained were subjected to one-way ANOVA and Scheffe's F test (P< 0.05: n=12 for each group). To evaluate the etching effect of phosphoric acid and the all-in-one adhesives, the treated enamel surfaces (37% phosphoric acid, 40% phosphoric acid, or all-in-one adhesives) were examined by FE-SEM. Results: The bond strengths of the two all-in-one adhesives tested to the polished enamel surface were significantly lower (P< 0.05) than to ground enamel. However, no significant differences (P> 0.05) were found between the polished and ground enamel surfaces for resin sealants using phosphoric acid. Under SEM examination, 37% or 40% phosphoric acid etched the enamel surface more than all-in-one adhesives.
  • Effects of incremental curing on contraction stresses associated with various resin composite buildups
    K Tachibana, T Kuroe, Y Tanino, N Satoh, N Ohata, H Sano, AA Caputo
    QUINTESSENCE INTERNATIONAL 35 4 299 - 306 2004年04月 [査読有り][通常論文]
     
    Objective: The purpose of this study was to photoelastically evaluate contraction stresses associated with various resin composite build-up procedures, including incremental curing and the use of flowable composite for pulpless molars. Method and materials: Life-sized photoelastic models of an endodontically treated molar were fabricated. The cavity represented a conservative access preparation and included four lateral walls. The following materials were used for buildup: dual-cured hybrid composite (Cleafil DC Core [DC], BIS-CORE [BC]); light-cured flowable composite (AELITEFLO LV [ALV]); and chemical-cured flowable composite (CORE-FLO [CF]). The photoelastic models were built up with the following techniques: bulk-cured (BDd [dual-cured DC], BDc [chemically-cured DC], BBd [dual-cured BC], and BC [CF]); and incremental-cured (11 mm gingivally + 3.5 mm occlusally; IAB [ALV + BC], ICB [CF + BC], and 11313 [BC + BC]). Isochromatic fringes developed in the models were recorded photographically in the field of a circular polariscope, and maximum fringe order was determined. Five specimens were tested for each condition. Results: Stress intensity of the build-up methods fell into two categories: high (BDd, BBd, BC, and IAB) and low (BDc, ICB, and IBB). The difference between high and low groups was statistically significant, except between BC and ICB. The maximum fringe order was developed around the point angles at the cavity floor for all the conditions tested. Conclusions: Type and setting mechanism of resin composite build-up technique had considerable influence on contraction stress. Incremental buildup using composites with low elastic modulus did not reduce contraction stress intensity compared with bulk-cured techniques.
  • Translucency of opaque-shade resin composites
    T Ikeda, Y Murata, H Sano
    AMERICAN JOURNAL OF DENTISTRY 17 2 127 - 130 2004年04月 [査読有り][通常論文]
     
    Purpose: To measure the translucency parameter of opaque-shade resin composites. The secondary purpose was to evaluate the ability of the materials to mask dark background color. Methods: CIELAB coordinates (L*, a*, b*) of three resin composites: UniFil S (UF), Palfique Estelite (PE), and Filtek A110 (FA) were determined in two shades (Opaque A3 and A3) at 1 mm and 2 mm thickness on a white backing, a backing of material itself and a black backing using a colorimeter. From the L* a* b* of 2 mm thick specimens on the white and black backing, translucency parameter (TP) were calculated for each material and shade to evaluate opacity of the materials. In addition, CIELAB color differences (DeltaE*) were also calculated from the L*, a*, b* of 1 mm and 2 mm thick specimens on the material itself and on black backing, in order to estimate effectiveness of the resin composite materials to mask dark background color. If the calculated DeltaE* were above 2, color differences were estimated as "perceptible." Results: The TP values of Opaque A3 shade resins were lower than those of A3 resins for all the materials evaluated. When comparing the materials used, the Opaque A3 shade of FA indicates a higher TP value than those indicated for the Opaque A3 shade of the other two materials, while the A3 shade of FA revealed a higher TP value than the A3 shade of UF. As for the DeltaE* values, the DeltaE* recorded for 1 mm-thick specimens were in the range of "perceptible" and larger than those of 2 mm specimens, regardless of the shades and materials. For the 2 mm specimens, all of the DeltaE* values for the Opaque A3 shade were classified as "imperceptible," whereas all DeltaE* values for Delta3 shade were evaluated as "perceptible."
  • E Yoshida, M Hashimoto, M Hori, M Kaga, H Sano, H Oguchi
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 68B 1 29 - 35 2004年01月 [査読有り][通常論文]
     
    This study investigated the effects of NaOCl on resin-tooth bonds to simulate the situations of long-term durability and caries invasion. Resin-tooth bonded specimens were produced with the use of two resin adhesives (Excite and One-Bond). Resin-tooth bonded beams (adhesive area; 0.9 mm(2)) were serially sectioned and the specimens were immersed in 10% NaOCl medium for 0 (control), 2, 4, and 6 h after being stored in water for 24 h. After immersion, microtensile bond tests were performed. SEM fractography was conducted to calculate each failure mode by image analysis. In addition, the adhesive interface was examined with the use of TEM. In the control specimens, enamel bond strengths had no difference between Excite (45.6 +/- 15.0) and One-Bond (56.9 +/- 12.9). On the other hand, dentin bond strengths had significant difference between Excite (80.6 +/- 21.2) and One-Bond (50.7 +/- 11.2). The bond strengths decreased with increased storage time for both systems with enamel and dentin bonds. The deteriorated mineralized dentin of beams resulted in bond-strength reduction for resin-enamel bonds. For dentin bonding, the adhesive interface was gradually dissolved from the outer to the center portion of the beam. The depletion of collagen fibrils within the demineralized dentin or hybrid layer deformation was found under SEM and TEM examinations. These morphological changes are responsible for bond strength reduction of resin-dentin bonds. (C) 2003 Wiley Periodicals, Inc.
  • Yoshida E, Hashimoto M, Hori M, Kaga M, Sano H, Oguchi H
    Journal of biomedical materials research. Part B, Applied biomaterials 68 1 29 - 35 2004年01月 [査読有り][通常論文]
  • Effect of bur-cut dentin on bond strength using two all-in-one and one two-step adhesive systems.
    Koase K, Inoue S, Noda M, Tanaka T, Kawamoto C, Takahashi A, Nakaoki Y, Sano H
    The journal of adhesive dentistry 6 2 97 - 104 2004年 [査読有り][通常論文]
  • In vitro sealing performance of two one-step adhesive systems in cervical cavities.
    Uno S, Abo T, Tanaka T, Sano H
    The journal of adhesive dentistry 6 3 211 - 219 2004年 [査読有り][通常論文]
  • EWS/ETS fusions activate telomerase in Ewing's tumors
    A Takahashi, F Higashino, M Aoyagi, K Yoshida, M Itoh, S Kyo, T Ohno, T Taira, H Ariga, K Nakajima, M Hatta, M Kobayashi, H Sano, T Kohgo, M Shindoh
    CANCER RESEARCH 63 23 8338 - 8344 2003年12月 [査読有り][通常論文]
     
    EWS/ETS is a chimeric protein identified in most Ewing's sarcomas. Although EWS/ETS has been shown to activate transcription as a transcription factor, the detailed targets of EWS/ETS in transformed cells have not been clarified. Herein, we demonstrate that telomerase is a new target of EWS/ETS fusions. Both telomerase activity and the expression level of telomerase reverse transcriptase (TERT) mRNA were up-regulated in NIH3T3 cells transformed by EWS/E1AF and EWS/FLI1 as well as in two Ewing's sarcoma cell lines. Luciferase assay using the TERT promoter revealed that EWS/E1AF and EWS/FLI1 function as positive regulators of TERT transcription in an ETS binding site-independent manner. EWS/ETS appeared to be included in the initiation complex of TERT transcription and to cooperate with CREB-binding protein (CBP)/ p300. When EWS/FLI1 was knocked down in Ewing's sarcomas cells by RNA interference, the expression level of TERT mRNA and the telomerase activity were significantly decreased. These findings indicate that EWS/ETS fusion proteins activate human telomerase activity in Ewing's tumors through up-regulation of TERT gene expression, probably as a transcriptional coactivator.
  • Color differences and color changes in Vita Shade tooth-colored restorative materials
    T Ikeda, A Nakanishi, T Yamamoto, H Sano
    AMERICAN JOURNAL OF DENTISTRY 16 6 381 - 384 2003年12月 [査読有り][通常論文]
     
    Purpose: To evaluate the color differences of four tooth-colored restorative materials of the same designated Vita shade, and the color changes of the materials caused by light curing. Methods: CIELAB coordinates (L* a* b*) of four tooth-colored materials: Clearfil ST (CL), Beautifil (BE), Reactmer (RE) and Z250 (Z2) were determined in three shades (A2, A3 and B3) before and after light curing using a colorimeter. From the L* a* b* after light curing, CIELAB color differences (DeltaE*) between different cured materials of the same shade name were calculated. If the calculated DeltaE* were above 2, color differences were estimated as "perceptible". DeltaE* was also calculated before and after light curing, using the CIELAB values, in order to evaluate color changes caused by light curing. Results: As for the DeltaE* between various cured materials of the same shade, except for DeltaE* between BE and RE on A2 and between BE and RE on A3, every combination indicated perceptible color differences. The DeltaE* calculated before and after light curing of A2, A3 and B3 shades were 7.4, 7.1 and 7.2 for CL, 6.0, 5.1 and 4.8 for BE, 4.0, 3.2 and 3.4 for RE and 1.8, 1.7 and 2.1 for Z2, respectively, indicating that most materials showed perceptible color changes.
  • Hg2+ and Ni2+ alter induction of heat shock protein-72 in THP-1 human monocytes
    M Noda, JC Wataha, JB Lewis, PE Lockwood, H Komatsu, H Sano
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 67A 1 240 - 245 2003年10月 [査読有り][通常論文]
     
    The biological liabilities that result from the release of metal ions from biomedical alloys, particularly Ni2+ and Hg2+, continue to be a concern. Heat-shock proteins (HSP) are a class of molecular chaperones that may be induced under conditions of cellular stress, including oxidative stress. Our hypothesis was that because Hg2+ and Ni2+ alter other cellular stress responses such as glutathione levels and cytokine secretion, these metal ions may alter HSP induction in monocytes, which are key cells in the response of tissues to biomedical alloys. THP-1 monocytes were exposed to sublethal concentrations of Hg2+ or Ni2+ for 1 h with or without heat stress (43degreesC), then allowed to recover at 37degreesC for 2-6 h. HSP72 was measured using immunoblotting with phosphorimage quantification. Hg2+ exposures of 2-10 mumol/l, induced HSP72 without heat stress. With heat stress, HSP72 levels were altered by Hg2+ versus heat stress alone. The response depended on the concentration of Hg2+ and the recovery time. Hg2+ at 10 mumol/L caused uniformly lower HSP72 levels. Ni2+ exposures of 20-100 mumol/L did not induce HSP72 without heat stress, but significantly altered heat-induced HSP72 expression, with a significant increase in expression over heat alone at 40 and 100 mumol/L. Results from the current study support the hypothesis that these metal ions can, at concentrations relevant to those released from biomedical alloys, modulate HSP expression in human monocytes. The modulation of HSP expression indicates an early sign of cellular stress that may be important to the overall biological response to biomedical alloys containing and releasing these metal ions. (C) 2003 Wiley Periodicals, Inc.
  • M Hashimoto, H Ohno, E Yoshida, M Hori, H Sano, M Kaga, H Oguchi
    EUROPEAN JOURNAL OF ORAL SCIENCES 111 5 447 - 453 2003年10月 [査読有り][通常論文]
     
    Self-etching primer adhesives have recently been introduced to simplify bonding. However, the fundamental bonding mechanism of self-etching primers to enamel is not yet fully understood. This study aimed to investigate resin-enamel bonds of self-etching primer adhesives on ground enamel. Two self-etching primer adhesives (Clearfil Liner Bond 2 V and Clearfil SE Bond) were used in this study. A total-etch adhesive (One-Step) was used as a control. Resin-enamel beams were subjected to the microtensile bond test. Subsequently, the failure modes of all specimens were quantified using image analysis. Undemineralized and demineralized ultrathin sections of the resin-enamel bonded specimens were examined using transmission electron microscopy. The bond strengths of Clearfil SE Bond (39.8+/-11.9 MPa) and One-Step (46.2+/-12.7 MPa) were significantly greater than that of Clearfil Liner Bond 2V (30.4+/-6.2 MPa). Scanning electron microscopy of the fractured surfaces revealed the failure direction and weakest portion within each bond. Transmission electron microscopy showed a thin hybridized complex of resin in enamel produced by the self-etching primers without the usual micrometer-sized resin tags seen in resin-enamel bonds produced using the total-etch adhesive. The morphological features of the resin-enamel bonds produced by two self-etching primers tested were different from that created with the total-etch adhesive.
  • M Aoyagi, F Higashino, M Yasuda, A Takahashi, Y Sawada, Y Totsuka, T Kohgo, H Sano, M Kobayashi, M Shindoh
    ONCOGENE 22 44 6919 - 6927 2003年10月 [査読有り][通常論文]
     
    The adenovirus E4orf6 is a viral oncoprotein known to cooperate with the E1A gene product in transforming primary murine cells. It has been shown to inhibit the apoptotic activities of p53 and p73 through direct binding to these proteins. Here, we demonstrate that the adenovirus E4orf6 protein inhibits apoptosis mediated by BNIP3 and Bik, which are BH3-only proteins of the Bcl-2 family. This activity was not mediated by p53 and p73 because E4orf6 had the same effect on the apoptosis in Saos-2 cells that do not express p53-related genes. It was also ascertained that E4orf6 could change the mitochondrial localization of BNIP3 and Bik. A mutant lacking the nuclear export signal of E4orf6 failed to inhibit apoptosis and to translocate BNIP3 protein from the mitochondria. Moreover, it was also established that E4orf6 was able to interact with BNIP3 and Bik. In BNIP3 protein, the region required for the interaction included the transmembrane domain, which is required for the localization of BNIP3 to the mitochondria. These results suggest that E4orf6 is exported from the nucleus to the cytoplasm, enabling it to interact with BH3-only proteins, eventually leading to the inhibition of apoptotic activity.
  • Microtensile bond strength of eleven contemporary adhesives to enamel
    S Inoue, MA Vargas, Y Abe, Y Yoshida, P Lambrechts, G Vanherle, H Sano, B Van Meerbeek
    AMERICAN JOURNAL OF DENTISTRY 16 5 329 - 334 2003年10月 [査読有り][通常論文]
     
    Purpose: To compare the microtensile bond strength (muTBS) to enamel of 10 contemporary adhesives, including three one-step self-etch systems, four two-step self-etch systems and three two-step total-etch systems, with that of a conventional three-step total-etch adhesive. Methods: Resin composite (Z100, 3M) was bonded to flat, #600-grit wet-sanded enamel surfaces of 18 extracted human third molars using the adhesives strictly according to the respective manufacturer's instructions. After storage overnight in 37degreesC water, the bonded specimens were sectioned into 2-4 thin slabs of approximately 1 mm thickness and 2.5 mm width. They were then trimmed into an hourglass shape with an interface area of approximately 1 mm(2), and subsequently subjected to muTBS-testing with a cross-head speed of 1 mm/minute. Results: The muTBS to enamel varied from 3.2 MPa for the experimental one-step self-etch adhesive PQ/Universal (self-etch) to 43.9 MPa for the two-step total-etch adhesive Scotchbond 1. When compared with the conventional three-step total-etch adhesive OptiBond FL, the bond strengths of most adhesives with simplified application procedures were not significantly different, except for two one-step self-etch adhesives, experimental PQ/Universal (self-etch) and One-up Bond F, that showed lower bond strengths. Specimen failures during sample preparation were recorded for the latter adhesives as well.
  • M Hashimoto, H Ohno, H Sano, M Kaga, H Oguchi
    BIOMATERIALS 24 21 3795 - 3803 2003年09月 [査読有り][通常論文]
     
    Our knowledge of the mechanisms responsible for the degradation of resin-dentin bonds are poorly understood. This study investigated the degradation of resin-dentin bonds after 1 year immersion in water. Resin-dentin beams (adhesive area: 0.9 mm(2)) were made by bonding using a resin adhesive, to extracted human teeth. The experimental beams were stored in water for I year. Beams that had been stored in water for 24 h were used as controls. After water storage, the beams were subjected to microtensile bond testing. The dentin side of the fractured surface was observed using FE-SEM. Subsequently, these fractured beams were embedded in epoxy resin and examined by TEM. The bond strength of the control specimens (40.3 +/- 15.1 MPa) decreased significantly (p < 0.01) after 1 year of water exposure (13.3 +/- 5.6 MPa). Loss of resin was observed within fractured hybrid layers in the I year specimens but not in the controls. Transmission electron microscopic examination revealed the presence of micromorphological alterations in the collagen fibrils after I year of water storage. These micromorphological changes (resin elution and alteration of the collagen fibrils) seem to be responsible for the bond degradation leading to bond strength reduction. (C) 2003 Elsevier Science Ltd. All rights reserved.
  • Konishi J, Watari F, Kawamoto C, Sano H
    Journal of biomedical materials research. Part B, Applied biomaterials 66 2 553 - 558 2003年08月 [査読有り][通常論文]
  • M Hashimoto, H Ohno, H Sano, M Kaga, H Oguchi
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 66B 1 324 - 330 2003年07月 [査読有り][通常論文]
     
    Various types of resin adhesives and procedures are available in the clinical field, so comprehensive understanding of degradation is required for each material and bonding procedure. The objective of this study was to investigate the bond durability for different adhesives and bonding procedures. Resin-dentin bonded beams were prepared with the use of two adhesives (One-Up Bond F/self-etching primer system and One Bond/total-etch adhesive) and two experimental groups for the bonding procedure (wet and dry bonding of the total-etch adhesive). Those samples were soaked in water for 24 h(control), 6 and 12 months. After the water immersion, the bond strengths were measured by the microtensile bond test, and subsequently fractography was performed with the use of SEM. Statistically significant reduction of the bond strength (P < 0.05) was apparent after 12 months of water exposure in the range 22-48% of the control. The bonding resin was eluted from the hybrid layer of the self-etching and the total-etch adhesives for the wet bonding. Micromorphological alterations were found due to the hydrolysis of collagen fibrils with the total-etch adhesive for the dry bonding mode. These pathologic alterations were in accord with the bond strength. (C) 2003 Wiley Periodicals, Inc.
  • M Hashimoto, FR Tay, H Ohno, H Sano, M Kaga, C Yiu, H Kumagai, Y Kudou, M Kubota, H Oguchi
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 66B 1 287 - 298 2003年07月 [査読有り][通常論文]
     
    Recently several long-term studies have reported evidence of the hydrolytic degradation of collagen fibrils based on fractured surface observations after bond testing. Those studies suggested that one cause of the decline in the bond strength was the degradation of the collagen fibrils within the bonds. However, one concern has been raised that the dentinal collagen fibrills may be stable in water that does not contain oral bacteria or enzymes. Therefore, the present study aimed to clarify the micromorphological change in naked collagen fibrils after 500 days of water storage. To prepare exposed collagen fibrils, sectioned and polished human dentin surfaces were acid conditioned for 15 s with the use of two commercially available acid conditioners: All-Etch (10% phosphoric acid) and Uni-Etch (32% phosphoric acid) (Bisco, Inc.). Those specimens were stored in distilled water at 37 degreesC for 1 day (control) for 500 days. After the storage periods, the samples were examined with the use of SEM and TEM. Under SEM and TEM examination, micromorphological alterations (disarrangement of collagen web, widening the interfibrillar space, and the thinning diameter of collagen fibrils) were found in the specimens after 500 days in water. (C) 2003 Wiley Periodicals, Inc.
  • Hashimoto M, Tay FR, Ohno H, Sano H, Kaga M, Yiu C, Kumagai H, Kudou Y, Kubota M, Oguchi H
    Journal of biomedical materials research. Part B, Applied biomaterials 66 1 287 - 298 2003年07月 [査読有り][通常論文]
  • Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H
    Journal of biomedical materials research. Part B, Applied biomaterials 66 1 324 - 330 2003年07月 [査読有り][通常論文]
  • M Noda, JC Wataha, PE Lockwood, KR Volkmann, M Kaga, H Sano
    DENTAL MATERIALS 19 2 101 - 105 2003年03月 [査読有り][通常論文]
     
    Objectives. The aim of this study was to investigate the hypothesis that dental material components alter cytokine secretion from monocytes if applied for several weeks at sublethal doses. The current study significantly extended exposure times of monocytes to the components over times published in previous studies. These exposure times approached the estimated average life span of monocytes in the bloodstream. Methods. Human THP-1 monocytes were exposed to 2-hydroxyethylmethacrylate (HEMA, 0-1.2 mmol/l), triethyleneglycoldimethacrylate (TEGDMA, 0-0.75 mmol/l), Hg2+ (0-2 mumol/l), or Ni2+ (0-20 mumol/l) for 2 weeks. The cells were then collected and additionally incubated for 24 It, with or without bacterial lipopolysaccharide (LPS), a common component of dental plaque. TNF-alpha secretion from THP-alpha was determined using by enzyme-linked immunosorbent assay. Results. None of the dental material components induced TNF-alpha from THP-1 by themselves, but LPS alone strongly induced TNF-alpha secretion as expected. HEMA and TEGDMA significantly suppressed (40-70%) TNF-alpha secretion from cells stimulated with LPS. Hg2+ at 2.0 mumol/l doubled TNF-alpha secretion from THP-1s stimulated with LPS over LPS alone. Ni2+ did not significantly affect TNF-alpha secretion, with or without LPS exposure. Significance. The results in this study suggest that sublethal, 2-week exposures of some dental material components may alter TNF-alpha secretion from THP-1 monocytes when the cells are challenged. These alterations may influence the biological response of tissues to materials in an inflammatory intraoral environment. (C) 2003 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.
  • S Inoue, PNR Pereira, C Kawamoto, M Nakajima, K Koshiro, J Tagami, RM Carvalho, DH Pashley, H Sano
    DENTAL MATERIALS JOURNAL 22 1 39 - 47 2003年03月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the effect of dentin depth and tubule direction on the ultimate tensile strength (UTS) of human dentin. Dentin slabs of 0.5-mm thickness were trimmed either from the mesial and distal (for specimens with the tubules parallel to the tensile force; parallel group) or from the occlusal and pulpal surfaces (perpendicular group) to reduce the cross-sectional area of the superficial, middle, and deep regions to 0.25 mm(2), and subjected to microtensile testing. From SEM photomicrographs of the fractured specimens of the parallel group, the tubule density was investigated. For both parallel and perpendicular groups, superficial dentin showed a significantly higher UTS than deep dentin. The tubule density of superficial dentin was significantly lower than that of middle and deep dentin. When performing the microtensile bond test to deep dentin, it is possible that cohesive failure of dentin can occur at relatively low tensile stresses.
  • KL Agee, EL Pashley, A Itthagarun, H Sano, FR Tay, DH Pashley
    DENTAL MATERIALS 19 1 60 - 68 2003年01月 [査読有り][通常論文]
     
    Objectives. The submicron hiatus represents a potential space between the base of the collagen network and the mineralized dentin when dentin is acid-etched for bonding. These spaces were observed in SEM studies after acid-etched dentin specimens were critical point dried or dehydrated in hexamethyidisilasane. However, they have never been identified in TEM studies of dentin hybrid layers. This study critically examined the cause of submicron hiati formation using a silver staining technique to measure nanoleakage. Methods. Two multi-step, total-etch adhesives (One-Step, Bisco; Single Bond, 3M) and two single-step, self-etching adhesives (Prompt L-Pop, ESPE; One-Up Bond F, Tokuyama) were examined. Flat dentin surfaces were bonded with these adhesives and a lining composite. In each adhesive group, 0.8 mm thick slabs from the same bonded tooth were coated with nail varnish applied 1 mm from the bonded interfaces. The varnish was either left to dry completely for 10 min before immersing in 50 wt% silver nitrate (AgNO3) for 24 h (group D), or painted on blotted tooth slabs that were immediately dropped into the AgNO3 solution (group M). After developing, undernineralized, unstained, epoxy resin-embedded sections were prepared for transmission electron microscopy (TEM) to identify the amount and distribution of silver uptake. Results. Nanoleakage patterns were observed in all adhesive-bonded teeth, regardless of brand. Fine reticular silver deposits were also found in the underlying undernineralized dentin. In group D, submicron hiati were seen as tunnels of heavy silver deposits beneath hybrid layers. Specifically, a hiatus occurred between the undernineralized intertubular dentin and a cohesively fractured layer of the same matrix that was attached to the base of the hybrid layer. Hiati were completely absent in group M, regardless of the brand of adhesive. Significance. Submicron hiati are artifacts created by desiccation during specimen processing, and should be referred to as such in future studies of bonded dentin interfaces. (C) 2002 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.
  • Contraction stress of composite resin build-up procedures for pulpless molars.
    Kuroe T, Tachibana K, Tanino Y, Satoh N, Ohata N, Sano H, Inoue N, Caputo AA
    The journal of adhesive dentistry 5 1 71 - 77 2003年 [査読有り][通常論文]
  • Effect of NaOCl treatment on bond strength between indirect resin core-buildup and dentin
    S Inoue, Y Murata, H Sano, T Kashiwada
    DENTAL MATERIALS JOURNAL 21 4 343 - 354 2002年12月 [査読有り][通常論文]
     
    The aim of this study was to investigate the bond strength between indirect resin core-buildup and dentin treated with or without hypochlorite (NaOCl) gel. The post-space was made in five extracted human molars with obturated root canals, and the indirect resin core was produced on a gypsum model. Then, the dentin surface was treated with/without NaOCl, followed by luting of the resin core into the cavity. After storage for 24 h in 37degreesC water, the specimens were sectioned for measuring bond strength to the root and coronal dentin, and were subjected to micro-tensile bond strength (MTBS) testing. Two-way ANOVA showed that significant differences in MTBS were revealed for both factors of NaOCl treatment and dentin region. It is concluded that, for indirect resin core-buildup, the proper use of the NaOCl gel to dentin could increase the bond strength.
  • M Hashimoto, H Ohno, H Sano, FR Tay, M Kaga, Y Kudou, H Oguchi, Y Araki, M Kubota
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH 63 3 306 - 311 2002年06月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the degradation of resin-dentin bonds after I year of water storage. Resin-dentin-bonded specimens were prepared with the use of an adhesive resin system (One-Step: Bisco). Half of the experimental specimens were sectioned perpendicular to the adhesive interface to produce a beam (adhesive area: 0.9 mm(2)) before being stored in distilled water at 37 degreesC for 1 year. The remaining half of the bonded specimens were sectioned into beams of similar dimensions after I year of water storage. Additional bonded specimens that had been stored in water for 24 h before sectioning into beams were used as controls. The beams in the two experimental groups and the control group were subjected to microtensile bond testing. Fractography was performed on all fractured beams with the use of FE-SEM. There were significant (p < .05) differences in bond strength among the control specimens (55.9 +/- 12.9 MPa), specimens that had been sectioned into beams after water storage (68.9 +/- 18.6 MPa), and specimens that had been sectioned into beams before water storage (28.1 +/- 9.3 MPa). Fractography revealed that the resin material was gradually extracted from the periphery to the center portion of the beam. This probably accounted for the decrease in bond strength after 1 year of water storage. (C) 2002 Wiley Periodicals, Inc.
  • Relation of enamel prism orientation to microtensile bond strength
    T Ikeda, S Uno, T Tanaka, S Kawakami, H Komatsu, H Sano
    AMERICAN JOURNAL OF DENTISTRY 15 2 109 - 113 2002年04月 [査読有り][通常論文]
     
    Purpose : To evaluate the effect of enamel prism orientation on microtensile bond strengths (MTBS) to enamel using two current bonding systems. Materials and Methods: The crown segments of extracted human third molars were cut mesio-distally to obtain buccal and lingual halves. One of the halves was cut horizontally at the middle of the crown to make a flat enamel surface parallel to prism orientation (parallel specimen). The other half was ground at the equator of the crown to produce a flat enamel surface perpendicular to the prism orientation (perpendicular specimen). Each surface was ground with 600 grit silicone carbide paper, treated with Clearfil Liner Bond IISigma (LB) or Single Bond (SB) according to the manufacturers' instructions and resin composite, AP-X or Z100, respectively, was built up onto it. After storage in water at 37 degreesC for 24 hours, all bonded specimens were sectioned and trimmed for measuring MTBS. In MTBS, the parallel specimens were stressed perpendicular to the prism long axis and the perpendicular specimens were stressed parallel to the prism axis. Results: MTBS of specimens stressed perpendicular to the prism long axis were 11.93 +/- 5.56 MPa for LB and 10.97 +/- 1.80 MPa for SB. MTBS of specimens stressed parallel to the prism axis in LB and SB were 19.07 +/- 6.35 MPa and 23.99 +/- 9.20 MPa, respectively. For each prism orientation, no difference in MTBS was found between the bonding systems (P > 0.05). Both systems showed lower MTBS in specimens stressed perpendicular to the prism long axis than in specimens stressed parallel to the prism axis.
  • Fractured surface characterization: wet versus dry bonding
    M Hashimoto, H Ohno, M Kaga, H Sano, K Endo, H Oguchi
    DENTAL MATERIALS 18 2 95 - 102 2002年03月 [査読有り][通常論文]
     
    Objective: Fractographic analysis was conducted to evaluate the resin-dentin bond structures made under wet and dry conditions. Methods: Resin-dentin bonded specimens were prepared using two adhesive resin systems (Single Bond/SB; 3M and All Bond 2/AB2; Bisco Inc) under wet and dry conditions. The specimens were sectioned perpendicular to the adhesive interface to produce a square bar-shaped specimen (adhesive area: 0.9 mm(2)) by means of a diamond saw. The mean bond tensile test was then conducted at a crosshead speed of 1.0 mm/min. The mean bond strengths were statistically compared with two-way ANOVA and Fisher's PLSD test (p < 0.05). Subsequently, the fractured surfaces of all specimens were examined using SEM and the area fractions of failure modes (%) were measured using an image analyzer on SEM microphotographs. Results: No significant differences in tensile-bond strength were observed between SB (60.1 +/- 16.4 MPa) and AB2 (69.8 +/- 17.4 MPa) (p > 0.05) under wet conditions. However, the bond strength either of SB or AB2 made under wet conditions was significantly greater than those made under dry conditions (SB: 26.2 +/- 12.5 MPa and AB2: 6.8 +/- 3.3 MPa) (p < 0.05). Under fractographic analysis, the major portion at the fractured surface was occupied by the cohesive failure of bonding resin and the resin composite for the wet conditions, and the top of the hybrid layer for the dry conditions in both systems. Significance: The interaction between the top of the hybrid layer and the bonding resin influenced the bond integrity. (C) 2002 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.
  • In vivo long-term durability of the bond to dentin using two adhesive systems.
    Takahashi A, Inoue S, Kawamoto C, Ominato R, Tanaka T, Sato Y, Pereira PN, Sano H
    The journal of adhesive dentistry 4 2 151 - 159 2002年 [査読有り][通常論文]
  • The glass-ionomer phase in resin-based restorative materials
    FR Tay, EL Pashley, C Huang, M Hashimoto, H Sano, RJ Smales, DH Pashley
    JOURNAL OF DENTAL RESEARCH 80 9 1808 - 1812 2001年09月 [査読有り][通常論文]
     
    Glass-ionomer (GI) fillers are added to restorative materials, but it is unclear if they truly react with these materials, This TEM study evaluated the existence of the GI phase in a conventional GIC (ChemFlex), a resin-modified GIC (Fuji II LQ, a giomer (Reactmer Paste), a compomer (Dyract AP), and a composite (Spectrum(TPH)), before and after water uptake. Wafers were stored at 100% RH for 24 hrs, or in water for 7 or 84 days. ChemFlex glass particles were surrounded by 300 nm-thick silica gel layers. In Fuji II LC, we found thinner hydrogel layers (100 nm) that became thicker upon water storage. No appreciable change occurred in Reactmer Paste. Only a very thin hydrogel layer occurred in Dyract AP, and none was seen in Spectrum(TPH) after water storage for 84 days. We conclude that the variable extent of the GI phase is determined by differences in the resin composition of the restoratives.
  • Resin-tooth adhesive interfaces after long-term function
    M Hashimoto, H Ohno, M Kaga, K Endo, H Sano, H Oguchi
    AMERICAN JOURNAL OF DENTISTRY 14 4 211 - 215 2001年08月 [査読有り][通常論文]
     
    Purpose: To characterize with scanning electron microscopy (SEM), the morphology of adhesive interfaces that had been functioning in an oral cavity for several years, Materials and Methods: Cavities were prepared in vivo in caries-affected primary molars under local anesthesia. After removal of the entire carious lesion, the cavity was restored with a dentin adhesive system (Scotchbond Multi-Purpose). After several yrs, as the successor permanent teeth erupted, the resin-filled teeth were extracted. Then, the extracted resin-restored primary molars were cross-sectioned perpendicular to the adhesive interface using a low speed diamond saw and then polished for SEM examination. Results: Morphological changes, such as digestion of the collagen fibrils and deterioration of the bonding resin and hybrid layer, were observed at the interface of the oral environment specimens. The results of this study demonstrated that degradation of resin-dentin bond structures appeared to occur in the human oral environment over time.
  • Bonding to caries-affected dentin using self-etching primers
    M Nakajima, M Ogata, M Okuda, J Tagami, H Sano, DH Pashley
    AMERICAN JOURNAL OF DENTISTRY 12 6 309 - 314 1999年12月 [査読有り][通常論文]
     
    Purpose: To evaluate resin bond strengths to caries-affected versus normal dentin using three self-etching primer adhesive systems. Materials and Methods: Carious lesions were excavated and then bonded with one of three self-etching primer adhesive systems (Clearfil Liner Bond 2, Clearfil Liner Bond 2V, A.R.T. Bond). A crown was then built up using a resin composite to a height of 4 mm. Five to six serial slabs approximately 0.7 mm thick were sectioned perpendicular to the bonded surface. The bonded caries-affected or normal dentin areas were isolated to create an hourglass configuration with a cross-sectional area of approximately 1 mm(2). The specimens were subjected to tensile stress at a crosshead speed of 1.2 mm/min. The bonded interfaces to caries-affected and normal dentin were examined with an SEM. Results: Clearfil Liner Bond 2 and Clearfil Liner Bond 2V produced high bond strengths to normal dentin, but significantly lower bonds than to caries-affected dentin. (P < 0.05). For A.R.T. Bond, there was no statistically significant difference between types of dentin. All three bonding systems produced thin (+/- 0.5-1.5 mu m) hybrid layers in normal dentin. When used on caries-affected dentin, all self-etching primer systems produced hybrid layers that were twice as thick as those produced in normal dentin.
  • Tensile bond strength to and SEM evaluation of ground and intact enamel surfaces
    N Kanemura, H Sano, J Tagami
    JOURNAL OF DENTISTRY 27 7 523 - 530 1999年09月 [査読有り][通常論文]
     
    Objectives: The aim of this study was to evaluate the bonding of four commercially available adhesive systems to ground and intact enamel surfaces. Methods: Extracted human teeth were used to measure the microtensile bond strength to enamel and a field-emission scanning electron microscopy (FE-SEM) was used to observe the bonded interface and the effect of the surface conditioning of each material. Intact buccal enamel surfaces were cleansed with tooth paste using a rotary dental brush, and the ground enamel surfaces were prepared by reducing approximately 0.5 mm from the buccal enamel surfaces using a high-speed diamond bur. One-Step (OS, Bisco), Single Bond (SB, 3M), Clearfil Liner Bond II (LBII, Kuraray), and Tokuso Mac Bond B (MBII, Tokuso) were evaluated for their ability to bond to enamel. Results: There was no significant difference in bond strengths between the materials when they were applied to ground enamel surfaces (p > 0.05). However, the bond strengths of the self-etching systems, LBII and MBII, had significantly lower bond strengths to intact enamel than the bonding systems OS and SB using phosphoric acid etching (p < 0.05). FE-SEM revealed that the etching pattern of self-etching primers was not deep enough to obtain good penetration of bonding resin when applied to intact enamel surfaces. Conclusions: Phosphoric acid etching produced good resin adhesion to ground and intact enamel. The self-etching/self-priming systems also produced good adhesion to ground enamel, but had lower bond strengths to intact enamel. (C) 1999 Elsevier Science Ltd. All rights reserved.
  • Effect of dentin primer application on regional bond strength to cervical wedge-shaped cavity walls
    J Tagami, M Nakajima, H Sano, J Tagami
    OPERATIVE DENTISTRY 24 2 81 - 88 1999年03月 [査読有り][通常論文]
     
    The purpose of this study was to examine the effect of multiple applications of self-etching primer on regional tensile bond strength (mu TBS) to artificial wedge-shaped cavities (i e, occlusal vs gingival wall). Eighteen extracted noncarious human molars were used to measure mu TBS, and four additional teeth were used for scanning electron microscopy (SEM) of the interface. Wedge-shaped defects were prepared in the buccal cervical dentin. The teeth were mounted on their distal surface, simulating the supine position of the teeth during dental treatment. The teeth were divided into two groups according to the bonding systems [Clearfil Liner Bond II (LB) or Imperva Fluoro Bond (PB)I, and again divided into two subgroups for bonding. One group (Group S) was treated once with the primer according to the manufacturer's instructions. For the other group (Group M), the primer was applied several times for the period recommended by the manufacturer. The adhesive bonding resins were then applied to the cavity walls, and restored with a low-viscosity resin composite (Protect Liner Fl, and stored in 37 degrees C water for 24 hours. The resin-bonded teeth were serially sliced parallel to the long axis of the tooth. The adhesive interface of each slice was trimmed alternately at the occlusal or gingival wall,and a microtensile testing method was used to compare resin bond strength to each wall. With both adhesive systems and primer application methods, tensile bond strength to the gingival wall was significantly lower than to the occlusal wall (P < 0.05). Tensile bond strength of LB to each cavity wall of a wedge-shaped defect increased significantly by multiple primer application (P < 0.05); however, bond strengths of FB were not significantly different after multiple primer applications.
  • Effect of intrinsic wetness and regional difference on dentin bond strength
    PNR Pereira, M Okuda, H Sano, T Yoshikawa, MF Burrow, J Tagami
    DENTAL MATERIALS 15 1 46 - 53 1999年01月 [査読有り][通常論文]
     
    Objectives: The aim of this, investigation was to determine the influence of intrinsic wetness on regional bond strengths of adhesive resins to dentin. Methods: Human caries-free third molars were randomly divided into three groups for-bonding: Group 1 - no pulpal pressure; Group 2 - pulpal pressure of 15 cm H2O; and Group 3 - dentin dried overnight in a desiccator. Clearfil Liner Bond Il (Kuraray) or One Step (Bisco) adhesive resins systems were applied to the flat dentin surfaces and the teeth were, restored with APX resin composite (Kuraray). After 24 h in water at 37 degrees C, the specimens were sectioned into 0.7 mm thick slabs and divided into three regional subgroups according to the remaining dentin thickness and visual criteria: pulp hem, center, and periphery. The slabs were then trimmed for the micro-tensile bond test and subjected to a tensile force and crosshead of 1 mm/min. The data were analyzed with ANOVA and Fisher's PLSD test at a confidence level of 95%. The fracture modes were determined under a scanning electron microscope (JXA-840, JEOL, Japan). Results: No significant regional difference was observed for the Group I and 2 specimens-restored with Clearfil Liner Bond II (p > 0.05), However, bond strengths significantly decreased at the pulp horn region of the Group 1 and 2 specimens restored with One Step (p < 0.01). All bond strengths of Group 3 decreased significantly and regional differences were not evident (p > 0.05), Significance: The dentin adhesive system should be chosen according to the substrate and region to be bonded, since bond strengths can vary according to the intrinsic wetness, region, and the adhesive system. (C) 1999 Published by Elsevier Science Ltd on behalf of the Academy of Dental Materials. All rights reserved.
  • Regional bond strengths of resins to human root dentine
    M Yoshiyama, RM Carvalho, H Sano, JA Horner, PD Brewer, DH Pashley
    JOURNAL OF DENTISTRY 24 6 435 - 442 1996年11月 [査読有り][通常論文]
     
    Objectives: The demand for restoration of root dentine defects such as cervical erosion and root caries has significantly increased recently, but there is limited information on the performance of the adhesive resins to radicular dentine. The purpose of this study was to measure the regional tensile bond strength (TBS) of composite bonded to human root and coronal dentine, and to observe the interface between resin and root dentine by SEM. Methods: Human extracted cuspid teeth were used to measure TBS with a new microtensile bond test. Enamel and cementum were removed from the labial tooth surfaces to form a long cavity preparation into dentine from the mid-crown to the apex of the root within the same tooth. All Bond 2 (all etch technique) or Imperva Bond (no etch) was bonded to dentine surfaces and covered with Protect Liner resin composite. The resin-bonded teeth were serially sliced into 16 sections at right angles to the long axis of the tooth, and the bonded surfaces were trimmed to give a bonded surface area of 1 mm(2) for TBS testing. Results: All Bond 2 bond strengths to coronal and apical dentine showed high values (23.5 MPa) but the bond strength was significantly lower on cervical root dentine. Imperva Bond produced a relatively high tensile bond strength to all regional areas. SEM showed that the thickness of the resin-infiltrated layer formed by All Bond 2 in root dentine was less than coronal areas. Resin infiltration with Imperva Bond was always less than 0.5 mu m. The results suggest that high TBS values can be obtained with minimal resin infiltration in root dentine. Copyright (C) 1996 Elsevier Science Ltd.
  • Regional strengths of bonding agents to cervical sclerotic root dentin
    M Yoshiyama, H Sano, S Ebisu, J Tagami, B Ciucchi, RM Carvalho, MH Johnson, DH Pashley
    JOURNAL OF DENTAL RESEARCH 75 6 1404 - 1413 1996年06月 [査読無し][通常論文]
     
    The regional bond strengths of three current-generation bonding systems (All Bond 2, Scotchbond Multi-Purpose, and Clearfil Liner Bond 2) were measured in natural wedge-shaped defects in the cervical area of extracted human teeth. A microtensile testing method was used to compare the strengths of resin bonds made to occlusal margins with those made to gingival margins. Controls consisted of normal teeth which had artificial wedge-shaped defects, of the same depth and dimension, created with a high-speed bur. The results indicated that there were no regional differences in bond strength, although bonds made to natural lesions were from 20 to 45% lower than those made to normal dentin in artificially created wedge-shaped defects, depending on the bonding agent. Scanning electron microscopy revealed that Clearfil Liner Bond 2 created the thinnest hybrid layers, which were difficult to measure in the natural lesions. The natural lesions contained sclerotic dentin, whereas the artificial lesions were composed of normal dentin. Although the bond strengths to sclerotic dentin were lower than those to normal dentin, the absolute values (ca. 16 to 17 MPa) were high relative to previous-generation bonding agents.
  • Dentin, a dynamic bonding substrate: The effects of dentin variables on resin adhesion
    DH Pashley, H Sano, M Yoshiyama, B Ciucchi, RM Carvalho
    DENTIN/PULP COMPLEX 11 - 21 1996年 [査読有り][通常論文]
     
    The effects of intrinsic variables such as dentin depth, pulpal pressure and surface wetness on dentin bond strength are less pronounced using the newest generation bonding systems compared to earlier formulations. However, there is a good deal of variability in the influence of bonding systems to intrinsic variables such as dentin depth. We have recently attempted to minimize this variability by using modeling approaches. A new mathematic model of dentin bonding indicates that the contribution of the hybrid layer to overall bond strength is highest in superficial dentin and lowest in deep dentin. Conversely, the contribution of resin tags to total bond strength is low in superficial dentin but high in deep dentin. This modeling approach to dentin bonding permits evaluation of single variables in a controlled manner and holds great promise in the future. Almost all published results on bond strengths deal with normal dentin yet, clinically, most dentin substrates include considerable sclerotic dentin. We have recently developed a microtensile bond testing method that permits measurement of tensile bond strengths in specimens as small as 0.5 mm(2). Using this technique, the bond strengths of current generation bonding systems produce tensile bond strengths to caries-affected dentin that are 20-40% lower than those made to normal dentin in the same tooth at the same depth. SEM observations indicate that the hybrid layers in caries-affected dentin are at least as thick if not thicker than those of normal dentin but resin tag formation was less frequent. Similar techniques have been used to evaluate bonding to natural wedge-shaped defects in cervical root dentin. In that study, bonds made to sclerotic cervical dentin were lower than those of artificial wedge-shaped defects prepared in normal cervical dentin. This was due, in part, to the absence of resin tags in sclerotic dentin.
  • Use of silver-nitrate to trace permeation of adhesive resin
    M Yoshiyama, S Ebisu, H Sano, RM Carvalho, DH Pashley, J Horner
    DENTIN/PULP COMPLEX 364 - 365 1996年 [査読有り][通常論文]
     
    The permeation of an experimental self-etching/self-priming adhesive system through the smear layer into underlying dentin was determined by transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS) of silver-nitrate as a tracer. Nine dentin disks 1 mm thick were prepared from the coronal middle dentin of extracted human third molars, and into the following three groups. In Group I, silver-nitrate (AgNO3) solution (10%) was applied on the smear layer-covered surface for 30 sec. In Group II, AgNO3 solution was applied on the etched dentin surface for 30 sec. In Group III, AgNO3-containing self-etching primer was applied on the smear layer-covered dentin surface for 30 sec. In Group I, Ag ions could penetrate only 0.5-1.0 mu m into the smear layer. In Group II, Ag ions could penetrate 4.0-8.0 mu m into the demineralized layer. In Group III, Ag ions in the primer could penetrate 2.0-3.0 mu m into underlying dentin through the smear layer. These results suggest that the use of silver-nitrate is very useful to trace the permeation of self-etching primers because of its high solubility and electron density, and indicate that the self-etching/self-priming system can create a special hybrid layer which contains the original smear layer.
  • Comparison of tensile bond strength to bovine crown and root dentin
    MF Burrow, H Sano, M Nakajima, N Harada, T Takatsu
    DENTIN/PULP COMPLEX 350 - 351 1996年 [査読有り][通常論文]
     
    The bond strengths of four commercial dentin bonding systems were tested and compared using bovine crown and root dentin. It was found that the bond strengths appeared to be dependent upon the bonding system used. All bond 2 showed a lower bond strength to crown dentin, and Liner Bond II showed the opposite. Both Probond and Super-Bond D-liner Plus showed no differences. These differences were thought to be dependent on the different bending mechanisms of each material, as well as variations in the crown and root dentin substrates.
  • Bond strength versus dentine structure: A modelling approach
    DH Pashley, B Ciucchi, H Sano, RM Carvalho, CM Russell
    ARCHIVES OF ORAL BIOLOGY 40 12 1109 - 1118 1995年12月 [査読有り][通常論文]
     
    Bond strengths of a hypothetical hydrophilic dentine-bonding agent were calculated as a function of dentine depth and resin strength to evaluate the importance of several variables in a simple model. The tested hypothesis was that the total bond strength was the sum of the strengths of resin tags, hybrid layer and surface adhesion. Each of these three variables has a range of values that can influence its relative contribution. The resulting calculations indicate the potential for higher bond strengths to deep dentine than to superficial dentine in non-vital dentine and the importance of resin strength in the development of strong bonds. Comparison of the calculated bonds with published values indicated that they were within the same order of magnitude. Such theoretical modelling of dentine bonding can identify the relative importance of variables involved in the substrate, resins and surface adhesion.
  • Tensile bond strength and SEM evaluation of caries-affected dentin using dentin adhesives
    M Nakajima, H Sano, MF Burrow, J Tagami, M Yoshiyama, S Ebisu, B Ciucchi, CM Russell, DH Pashley
    JOURNAL OF DENTAL RESEARCH 74 10 1679 - 1688 1995年10月 [査読無し][通常論文]
     
    Tensile bond strength measurements are commonly used for the evaluation of dentin adhesive systems. Most tests are performed using extracted non-carious human or bovine dentin. However, the adhesion of resins to caries-affected dentin is still unclear. The objectives of this study were to test the hypothesis that bonding to caries-affected dentin is inferior to bonding to normal dentin, and that the quality of the hybrid layer plays a major role in creating good adhesion. We used a micro-tensile bond strength test to compare test bond strengths made to either caries-affected dentin or normal dentin, using three commercial adhesive systems (All Bond 2, Scotchbond Multi-Purpose, and Clearfil Liner Bond II). For scanning electron microscopy, the polished interfaces between the adhesive bond and dentin were subjected to brief exposure to 10% phosphoric acid solution and 5% sodium hypochlorite, so that the quality of the hybrid layers could be observed. Bonding to normal dentin with either All Bond 2 (26.9 +/- 8.8 MPa) or Clearfil Liner Bond II (29.5 +/- 10.9 MPa) showed tensile bond strengths higher than those to caries-affected dentin (13.0 +/- 3.6 MPa and 14.0 +/- 4.3 MPa, respectively). The tensile bond strengths obtained with Scotchbond Multi-Purpose were similar in normal and caries-affected dentin (20.3 +/- 5.5 MPa and 18.5 +/- 4.0 MPa, respectively). The hybrid layers created by All Bond 2 in normal dentin and by Clearfil Liner Bond II in normal or caries-affected dentin showed phosphoric acid and sodium hypochlorite resistance, whereas the hybrid layers created by All Bond 2 in caries-affected dentin and those created by Scotchbond Multi-Purpose to normal and caries-affected dentin showed partial susceptibility to the acid and sodium hypochlorite treatment. The results indicate that the strength of adhesion to dentin depends upon both the adhesive system used and the type of dentin. Moreover, the quality of the hybrid layer may not always contribute significantly to tensile bond strength.
  • ADHESION TESTING OF DENTIN BONDING AGENTS - A REVIEW
    DH PASHLEY, H SANO, B CIUCCHI, M YOSHIYAMA, RM CARVALHO
    DENTAL MATERIALS 11 2 117 - 125 1995年03月 [査読有り][通常論文]
     
    Adhesion testing of dentin bonding agents was reviewed starting with the adhesion substrate, dentin, the variables involved in etching, priming and bonding, storage variables and testing variables. Several recent reports attempting to standardize many of these variables were discussed. Recent advances in the development of new bonding systems have resulted in bond strengths on the order of 20-30 MPa. At these high bond strengths, most of the bond failure modes have been cohesive in dentin. As this precludes measurement of interfacial bond strength, new testing methods must be developed. One such new method, a microtensile method, was described along with preliminary results that have been obtained. The last decade has produced major advances in dentin bonding. The next decade should prove to be even more exciting.

書籍

  • Clinical consideration of dentin adhesion
    Modern trends in adhesive dentistry 1998年

講演・口頭発表等

  • 接着歯学の潮流 最近の研究成果から Microtensile bond testを行う場合に考えるべきこと  [通常講演]
    佐野 英彦
    接着歯学 2018年08月
  • S-PRGバーニッシュによる象牙質表面の脱灰抑制効果  [通常講演]
    松田 康裕, 奥山 克史, 山本 洋子, 大木 彩子, 泉川 昌宣, 伊藤 修一, 林 美加子, 佐野 英彦, 斎藤 隆史
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 2018年05月
  • 白金ナノコロイドを用いたユニバーサルボンドの象牙質接着強さ  [通常講演]
    勝俣 環, 勝俣 愛一郎, 星加 知宏, 梶原 武弘, 佐野 英彦, 西谷 佳浩
    日本再生歯科医学会誌 2017年12月
  • フッ化物含有知覚過敏抑制材による象牙質表面の脱灰抑制効果  [通常講演]
    松田 康裕, 奥山 克史, 山本 洋子, 大木 彩子, Khatun Morsheda Mosammat, 佐野 英彦, 斎藤 隆史
    日本歯科保存学雑誌 2017年12月 
    目的:象牙質知覚過敏症に対してさまざまなフッ化物含有知覚過敏抑制材がすでに臨床応用されており、それによる脱灰抑制効果も期待されている。また高齢化社会への変化に伴って根面う蝕が増加しており、根面う蝕に対する予防法および治療方法の確立も求められている。そこで、今回は脱灰処理をした象牙質にフッ化物含有知覚過敏抑制材を塗布し、歯質へのフッ素の取り込みと脱灰抑制効果について、PIGE/PIXEと自動pHサイクル装置を用いて検討を行った。材料と方法:フッ化物含有知覚過敏抑制材として、MSコートF(MS)、MSコートHySブロックジェル(HS)とCTx2 Varnish(FV)を使用した。観察試料は、ヒト抜去大臼歯を7本使用し(n=7)、37℃の脱灰溶液(0.2mol/l乳酸、3.0mmol/l CaCl2,1.8mmol/l KH2PO4,pH 4.5,2%カルボキシメチルセルロースナトリウム)を用いて72時間脱灰した後に、それぞれ頬舌的・近遠心的に切断して4分割した。分割試料は歯面のCEJを挟んで約3mmの幅を残して、スティッキーワックスで約1mmの厚さになるように被覆した。各歯の3分割試料にMS,HS,FVそれぞれの材料を塗布し、残りの1分割試料は材料を塗布しないコントロール(CONT)とした。37℃の脱イオン水中に24時間浸漬後、塗布部位が含まれるように歯軸に平行に切断し、厚さ約300μmに調整し試料を作製した。象牙質表層に取り込まれたフッ素はIn-air μPIXE/PIGEを用いて測定を行った。その後、材料を塗布した面を除き、すべての歯面をスティッキーワックスで被覆して、Single-section試料とした。これまでの報告と同様に、自動pHサイクル装置を用いて脱灰負荷試験を行った。各試料のTransverse Microradiography(TMR)を、実験開始前、pHサイクル2週後に撮影し、得られた画像からCEJに近接した象牙質の脱灰量の変化を検討した。2週後におけるIntegrated Mineral Loss(IML)の増加量(ΔIML)について、Games-Howelの多重比較検定(p<0.05)を用いて統計解析を行った。結果:フッ素の取り込みでは、CONTと比較してすべての試料使用群でフッ素取り込み量の増加傾向が認められたが、なかでもHS群では有意に増加していた。ΔIMLによる脱灰抑制効果の分析では、CONTと比較してHS・FV群で有意に抑制効果が認められたが、MS群では有意差はなかった。考察:今回の結果から、脱灰処理を行った象牙質に使用した3種類のフッ化物含有知覚過敏抑制材からフッ素が取り込まれる傾向が認められた。また、それらのフッ素によって象牙質の脱灰が抑制されることが示された。これらの結果から、フッ化物含有知覚過敏抑制材の根面う蝕予防材としての有効性が示唆された。(著者抄録)
  • ナノ構造体シリカからの薬剤徐放挙動と生体適合性の評価  [通常講演]
    阿部 薫明, 中西 康, 坂東 洋祐, 成徳 英理, 江良 裕子, 飯田 順一郎, 佐野 英彦, 吉田 靖弘
    日本バイオマテリアル学会大会予稿集 2017年11月
  • フッ化物含有知覚過敏抑制材によるエナメル質表面の脱灰抑制効果  [通常講演]
    櫻井 雅彦, 松田 康裕, 奥山 克史, 山本 洋子, カトゥーン・モーサマット・モーシーダ, 佐野 英彦, 斎藤 隆史
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 2017年10月
  • 残存象牙質の厚みとスミヤー層の違いが象牙質微小接着引張接着試験に及ぼす影響(Effect of remaining dentin thickness and smear layer thickness on microtensile bond strength)  [通常講演]
    リミ・シャンミ・アカター, アハメッド・ズバエル, 丁 世俊, サイケオ・ピポップ, イアムサアード・ピンピニー, チョウドリー・アルマス, イスラム・ラフィクル, 戸井田 侑, 松本 真理子, 角田 晋一, 星加 修平, 川本 千春, 池田 考績, 田中 享, 佐野 英彦
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 2017年10月
  • 新規レジンセメントの象牙質接着性能評価(Evaluation of A Newly Developed Resin Cement on Dentin Bonding)  [通常講演]
    イアムサアード・ピンピニー, 松本 真理子, 丁 世俊, サイケオ・ピポップ, リミ・シャンミン・アクター, アハメッド・ズバエル, デニス・セリモビッチ, 佐野 英彦
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 2017年05月
  • オフィスブリーチング後のエナメル質に対するフッ素取り込み  [通常講演]
    福山 麻衣, 川本 千春, 大木 彩子, 松田 康裕, 奥山 克史, 山本 洋子, デニス・セリモビッチ, 佐野 英彦
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 2017年05月
  • ヒト由来細胞に対するセラミックナノ粒子の生体適合性の検討  [通常講演]
    阿部 薫明, 成徳 英理, 江良 裕子, 吉田 靖弘, 佐野 英彦
    日本バイオマテリアル学会大会予稿集 2016年11月
  • SOELL Martine, SOELL Martine, NAGANO Futami, SANO Hidehiko, SELIMOVIC Denis, SELIMOVIC Denis
    接着歯学 2012年08月
  • WANG Ying, KAKUDA Shinichi, FU Jiale, KAGA Masayuki, SANO Hidehiko, YAWAKA Yasutaka
    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 2012年05月
  • WANG Y, FU J, 加我正行, 加我正行, 橋本正則, 佐野英彦, 八若保孝
    接着歯学 2011年12月
  • TING Shih Chun, FU Jiale, PAN Feng, KAKUDA Shinichi, NAKAOKI Yasuko, IKEDA Takatsumi, TANAKA Toru, SANO Hidehiko
    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 2011年09月
  • FU Jiale, PAN Feng, TING Shih‐chun, KAKUDA Shinichi, KAWANO Shinpei, HASHIMOTO Naoki, FUKUOKA Anri, MATSUDA Yasuhiro, KAWAMMOTO Chiharu, NAKAOKI Yasuko, IKEDA Takatsumi, INOUE Satoshi, TANAKA Toru, KOMATSU Hisanori, SANO Hidehiko
    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 2011年09月
  • SANO Hidehiko
    Nano Biomed 2011年06月
  • NAKAOKI Yasuko, FUKUOKA Anri, IGARASHI Yutaka, FU Jiale, KAKUDA Shinichi, MATSUDA Yasuhiro, OKUYAMA Katsushi, KAWAMOTO Chiharu, IKEDA Takatsumi, TANAKA Toru, INOUE Satoshi, KOMATSU Hisanori, SANO Hidehiko
    Nano Biomed 2011年06月
  • TING Shihchun, FU Jiale, PAN Feng, KAKUDA Shinichi, NAKAOKI Yasuko, IKEDA Takatsumi, TANAKA Toru, SANO Hidehiko
    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 2011年05月
  • FU Jiale, TING Shihchun, PAN Feng, KAKUDA Shinichi, NAKAOKI Yasuko, IKEDA Takatsumi, TANAKA Toni, SANO Hidehiko
    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 2011年05月
  • KACHI Hanako, NODA Mamoru, WATAHA John C, NAKAOKI Yasuko, SANO Hidehiko
    J Biomed Mater Res Pt B Appl Biomater 2011年02月
  • INOUE Satoshi, MURATA Yasuhiro, SANO Hidehiko, KASHIWADA Toshiaki
    Dental materials journal 2002年 
    The aim of this study was to investigate the bond strength between indirect resin core-buildup and dentin treated with or without hypochiorite (NaOCl) gel. The post-space was made in five extracted human molars with obturated root canals, and the indirect resin core was produced on a gypsum model. Then, the dentin surface was treated with/without NaOCl, followed by luting of the resin core into the cavity. After storage for 24 h in 37℃ water, the specimens were sectioned for measuring bond strength to the root and coronal dentin, and were subjected to micro-tensile bond strength (MTBS) test...
  • Urabe Isao, Sano Hidehiko, Tagami Junji
    日本硬組織研究技術学会雑誌 1998年10月 
    To define the physical properties of the dentino-enamel junction (DEJ), we measured the ultimate tensile strength using a microtensile test, and measured nanohardness and Young's modulus using a nanoindentation tester. The ultimate tensile strength was 51.5 MPa, and the values of nanohardness and Young's modulus showed moderatevalues between enamel and dentin. Observation of the fractured surfaces revealed that the bonding properties between enamel and dentin is very high.
  • SANO Hidehiko, KANEMURA Nobuharu, BURROW Michael. F, INAI Norimichi, YAMADA Toshimoto, TAGAMI Junji
    Dental materials journal 1998年03月 
    The purpose of this study was to evaluate the operator variability of two dentin bonding systems. Fourteen undergraduate students and ten dentists of different levels of clinical experience were selected. Tensile bond strengths between resin composite and bovine dentin using dentin adhesive systems (Clearfil Liner Bond II: LB II; Scotchbond Multi-Purpose: MP) bounding systems showed a large scatter among students and dentists. The dentists group demonstrated no statistically significant differences for mean bond strengths between LB II and MP. The student group recorded statistically greate...
  • YOSHIYAMA Masahiro, SANO Hidehiko, CARVALHO Ricardo M, PASHLEY David.H
    Journal of hard tissue biology 1996年03月 
    Self-etching/self-primlng adhesive systems have recently been marketed to simplify the dentin bonding procedure by combining the etching and priming steps. The purpose of this study was to measure the regional tensile bond strength (TBS) of various portions of human tooth enamel and dentin, and to observe by scanning electron microscopy (SEM) the resin-dentin interfaces after applying an experlmental self-etching/self-priming adhesive system. Five human extracted cuspid teeth were used to measure TBS with a micro-tensile bond test. The outer enamel was removed from the incisal tooth surface...
  • レジン系修復物の辺縁漏洩に関する研究 -窩壁適合性と漏洩の関係についての検討  [通常講演]
    日本歯科保存学雑誌 1993年
  • 保存修復学における学生の修得能力および各評価要素間の相関性  [通常講演]
    日本歯科医学教育学会雑誌 1991年
  • コンポジットレジンの分類とフィラー組成に関する研究 第1報 化学重合レジンの分類  [通常講演]
    日本歯科保存学雑誌 1987年
  • 臼歯修復用のコンポジットレジンの3年並びに4年後の臨床成績  [通常講演]
    日本歯科保存学雑誌 1987年
  • 臼歯修復用コンポジットレジンの2年後の臨床成績  [通常講演]
    日本歯科保存学雑誌 1985年

その他活動・業績

  • Abe Shigeaki, Kawano Shimpei, Toida Yu, Nakamura Mariko, Inoue Satoshi, Sano Hidehiko, Yoshida Yasuhiro, Kawabata Hiroshi, Tachikawa Hiroto Jpn. J. Appl. Phys. 55 (3) 2016年01月19日 [査読無し][通常論文]
     
    The structures and electronic states of alkyl-radical-functionalized C<inf>20</inf>fullerenes (denoted by C<inf>20</inf>–R) have been investigated using density functional theory (DFT). The different alkyl radicals investigated were methyl, ethyl, propyl, and butyl radicals. The DFT calculation indicated that the alkyl radical binds to the carbon atom of C<inf>20</inf>in the on-top site, thus forming a strong C–C single bond. The binding energies of the alkyl radicals to C<inf>20</inf>were calculated to be 83.9–86.6 kcal/mol at the CAM-B3LYP/6-311G(d,p) level. The electronic states of the C<inf>20</inf>–R complex are discussed on the basis of the theoretical results.
  • 松田康裕, 松田康裕, 奥山克史, 奥山克史, 山本洋子, 小松久憲, 江夏昌志, 佐藤隆博, 佐野英彦, 安田啓介, 安田啓介, 鈴木耕拓, 林美加子, 能町正治, 菅谷頼仁 若狭湾エネルギー研究センター研究年報 17 33 -34 2015年10月 [査読無し][通常論文]
  • 奥山克史, 奥山克史, 山本洋子, 松田康裕, 松田康裕, 小松久憲, 佐野英彦, 安田啓介, 安田啓介, 鈴木耕拓, 林美加子, 能町正治, 菅谷頼仁 若狭湾エネルギー研究センター研究年報 17 31 -32 2015年10月 [査読無し][通常論文]
  • 奥山克史, 松田康裕, 小松久憲, 山本洋子, 安田啓介, 佐野英彦, 能町正治, 菅谷頼仁 若狭湾エネルギー研究センター研究年報 16 30 -31 2014年10月 [査読無し][通常論文]
  • 奥山克史, 松田康裕, 山本洋子, 小松久憲, 安田啓介, 佐野英彦, 能町正治, 菅谷頼仁, 佐藤隆博, 江夏昌志 若狭湾エネルギー研究センター研究年報 16 32 2014年10月 [査読無し][通常論文]
  • 奥山克史, 小松久憲, 山本洋子, 安田啓介, 船戸良基, 木地村太基, 佐野英彦 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 137th P70 (WEB ONLY) 2012年10月23日 [査読無し][通常論文]
  • 中沖靖子, 福岡杏理, 角田晋一, FU Joale, 星加修平, 松田康裕, 奥山克史, 川本千春, 池田考績, 田中享, 井上哲, 小松久憲, 野田守, 佐野英彦 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 134th 48 (WEB ONLY) -48 2011年05月16日 [査読無し][通常論文]
  • 小松久憲, 山本洋子, 安田啓介, 船戸良基, 小島健太郎, 木地村太基, 奥山克史, 佐野英彦 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 132nd P24 (WEB ONLY) 2010年05月21日 [査読無し][通常論文]
  • Takashi Nakata, Motoaki Yasuda, Mari Fujita, Hideo Kataoka, Kazuto Kiura, Hidehiko Sano, Kenichiro Shibata CELLULAR MICROBIOLOGY 8 (12) 1899 -1909 2006年12月 [査読無し][通常論文]
     
    It has demonstrated that the recognition of triacylated lipopeptides by Toll-like receptor (TLR) 2 requires TLR1 as a coreceptor. In the NF-kappa B reporter assay system in which human embryonic kidney 293 cells were transfected with TLR2 and TLR1 together with an NF-kappa B luciferase reporter gene, S-(2,3-bispalmitoyloxy-propyl)N-palmitoyl-Cys-Lys-Lys-Lys-Lys (Pam(3)CSK(4)) and Pam(3)CSSNA were recognized by TLR2/TLR1, but the recognition level was unexpectedly very low. However, cotransfection of CD14 drastically enhanced the recognition of triacylated lipopeptides by TLR2/TLR1. The CD14-induced enhancement did not occur without cotransfection of TLR1. Both CD14(dS39-A48), a mutant with deletion of the part of possible N-terminal ligand-binding pocket, and anti-CD14 monoclonal antibody reduced the CD14-induced enhancement. Transfection of a TIR domain-deficient mutant of TLR2 (TLR2(dE772-S784)) or TLR1 (TLR1(dQ636-K779)) completely abrogated the CD14-induced enhancement. Soluble recombinant CD14 added extracellularly enhanced the recognition of Pam(3)CSSNA by TLR2/TLR1. Immunoprecipitation analysis demonstrated that CD14 was not associated with TLR2 but that TLR1 was associated with TLR2. In addition, surface plasmon resonance-based assay demonstrated that CD14 binds to Pam(3)CSK(4) at a dissociation constant of 5.7 mu M. This study suggests that CD14 directly binds to triacylated lipopeptides and facilitates recognition of the lipopeptides by the TLR2/TLR1 complex without binding to the receptor complex.
  • M Hashimoto, J De Munck, S Ito, H Sano, M Kaga, H Oguchi, B Van Meerbeek, DH Pashley BIOMATERIALS 25 (25) 5565 -5574 2004年11月 [査読無し][通常論文]
     
    This study evaluated the effect of multiple consecutive adhesive resin coatings of adhesive bonded to human dentin oil nanoleakage and resin-dentin bond strength. Resin bonded dentin specimens were prepared using a total-etch adhesive (One-Step Plus) applied as multiple consecutive coating, or using two self-etch adhesive systems (iBond or Fluoro Bond). For the total-etch adhesive, resin application and air evaporation were performed 1, 2. 3, or 4 times. The self-etch adhesives were applied according to manufacturers' instructions. Resin-dentin bonded beams were prepared and immersed in water (control) or ammoniacal silver nitrate. After storage, microtensile bond strengths were measured. The fractured surfaces were examined by scanning and transmission electron microscopy (SEM and TEM), and energy-dispersive X-ray spectrometry (EDX). No significant differences in bond strength were found between water and silver nitrate storage groups. Several types of silver depositions (spotted, reticular, or water trees) were found in adhesive joints. The bond strengths of the single coated specimens of the total-etch adhesive were significantly lower than those receiving 2-4 coatings. Single coats produced more nanoleakage than multiple coats. However, no correlation was found between the bond strengths and nanoleakage between the different adhesives (total-etch adhesive with different conditions or self-etch adhesives). (C) 2004 Elsevier Ltd. All rights reserved.
  • Bond strength of simplified-step adhesives to enamel prepared with two different diamond burs
    F Sevgican, S Inoue, K Koase, C Kawamoto, T Ikeda, H Sano AUSTRALIAN DENTAL JOURNAL 49 (3) 141 -145 2004年09月 [査読無し][通常論文]
     
    Background: Self-etching primers are reported to produce considerable etching on flat enamel surfaces. However, little is known about the influence of different enamel surface textures. In this study the influence of grinding enamel on bond strength of two all-in-one and two-step adhesives was investigated. Methods: Resin composite was bonded to the ground enamel of extracted human third molars that was reduced 0.5mm from the buccal or lingual surfaces using either regular or superfine-grit diamond burs with each of the three adhesives. After 24 hours in 37degreesC water, the specimens were sectioned into slabs of 0.7mm thickness, trimmed to an hour glass configuration, and subjected to micro-tensile bond strength (MTBS) testing. Results: For all adhesive systems, MTBS to enamel ground with a regular-grit diamond bur was not significantly different from that with a superfine grit diamond bur. The etching patterns of there adhesives were partly varied according to the aggressiveness of the adhesives. Conclusions: The use of two different burs does not affect the tensile bond strength of the adhesives to enamel.
  • In vivo degradation of resin-dentin bonds produced by a self-etch vs. a total-etch adhesive system
    K Koshiro, S Inoue, T Tanaka, K Koase, M Fujita, M Hashimoto, H Sano EUROPEAN JOURNAL OF ORAL SCIENCES 112 (4) 368 -375 2004年08月 [査読無し][通常論文]
     
    The purpose of this study was to evaluate the long-term durability of in vivo bond strengths and the morphological changes of interfaces between dentin and two adhesive systems. Class V preparations were prepared on the facial surfaces of 14 intact teeth of two monkeys and restored with a combination of Unifil Bond/Z250 or Single Bond/Z250. One year later, 10 additional teeth were restored with the same materials and the monkeys were killed after 24 h. All of the restored teeth were subjected to microtensile bond strength (muTBS) testing. The debonded surfaces of the dentin sides were morphologically observed using Fe-scanning electron microscopy (SEM), as were the polished cross-sections of resin-lentin interfaces. For both Unifil Bond and Single Bond, the muTBS at 24 h was significantly higher than that at 1 yr. Fe-SEM observations of polished cross-sectioned and fractured surfaces showed that porosity within the hybrid layers produced by Single Bond increased over time. However, the interface produced by Unifil Bond revealed no noticeable changes in morphology between 24-h and 1-yr specimens. It is concluded that even though the bond strengths of both adhesive systems declined over time, the bonding interface using self-etching primers was relatively stable over time compared to the wet bonding system.
  • T Abo, S Uno, H Sano EUROPEAN JOURNAL OF ORAL SCIENCES 112 (3) 286 -292 2004年06月 [査読無し][通常論文]
     
    The aim of this study was to compare the bonding efficacy of an all-in-one adhesive with that of a self-etching primer system by measuring microleakage after thermocycling and the microtensile bond strength (muTBS) to enamel and dentin. Circular class V cavities along cement-enamel junctions were prepared in 20 extracted human premolars. Each 10 teeth were bonded with either AD Bond (AD) or Clearfil SE Bond (SE), and filled with a resin composite (Charisma). After thermocycling and staining with 0.5% basic fuchsin, the microleakage at the coronal and apical walls was evaluated using longitudinal sections. In addition 20 premolars were used to measure muTBS at 24 h after bonding for these adhesives to enamel and dentin that corresponded to the coronal and apical walls of the class V cavities. AD showed more microleakage than SE in the coronal walls, but there was less microleakage in the apical walls and no difference in apical leakage between the two adhesives. muTBS (SD) in MPa to enamel and dentin were 25.2 (7.3) and 68.3 (9.4) for AD, and 35.8 (7.4) and 76.4 (7.8) for SE, respectively. AD gave a lower muTBS to enamel than did SE. The results suggested that the poor adaptation at the coronal wall in AD might be caused by the lower bond strength to enamel.
  • Effects of incremental curing on contraction stresses associated with various resin composite buildups
    K Tachibana, T Kuroe, Y Tanino, N Satoh, N Ohata, H Sano, AA Caputo QUINTESSENCE INTERNATIONAL 35 (4) 299 -306 2004年04月 [査読無し][通常論文]
     
    Objective: The purpose of this study was to photoelastically evaluate contraction stresses associated with various resin composite build-up procedures, including incremental curing and the use of flowable composite for pulpless molars. Method and materials: Life-sized photoelastic models of an endodontically treated molar were fabricated. The cavity represented a conservative access preparation and included four lateral walls. The following materials were used for buildup: dual-cured hybrid composite (Cleafil DC Core [DC], BIS-CORE [BC]); light-cured flowable composite (AELITEFLO LV [ALV]); and chemical-cured flowable composite (CORE-FLO [CF]). The photoelastic models were built up with the following techniques: bulk-cured (BDd [dual-cured DC], BDc [chemically-cured DC], BBd [dual-cured BC], and BC [CF]); and incremental-cured (11 mm gingivally + 3.5 mm occlusally; IAB [ALV + BC], ICB [CF + BC], and 11313 [BC + BC]). Isochromatic fringes developed in the models were recorded photographically in the field of a circular polariscope, and maximum fringe order was determined. Five specimens were tested for each condition. Results: Stress intensity of the build-up methods fell into two categories: high (BDd, BBd, BC, and IAB) and low (BDc, ICB, and IBB). The difference between high and low groups was statistically significant, except between BC and ICB. The maximum fringe order was developed around the point angles at the cavity floor for all the conditions tested. Conclusions: Type and setting mechanism of resin composite build-up technique had considerable influence on contraction stress. Incremental buildup using composites with low elastic modulus did not reduce contraction stress intensity compared with bulk-cured techniques.
  • Akiko Takahashi, Fumihiro Higashino, Mariko Aoyagi, Satoru Kyo, Takashi Nakata, Mamoru Noda, Masanobu Shindoh, Takao Kohgo, Hidehiko Sano. : Bisphenol A from dental polycarbonate crown upregulates the expression of hTERT J Biomed Mater Res.
    15 ; 71B(1) : 214-21 2004年 [査読無し][通常論文]
     
    2004 Oct
  • Hori M, Yoshida E, Hashimoto M, Kaga M, Sano H, Oguchi H. : In vitro testing of all-in-one adhesives as fissure sealants.
    Am J Dent. 17(3) : 177-81 2004年 [査読無し][通常論文]
     
    2004 Jun
  • Kaori Koase, Satoshi Inoue, Mamoru Noda, Toru Tanala, Chiharu Kawamoto, Akiko Takahashi, Yasuko Nakaoki, Hidehiko Sano. : Effect of Bur-cut Dentin on Bond Strength Using Two All-in-one and One Two-step Adhesive Systems.
    J Adhes Dent No. 5(2), 97-104 2004年 [査読無し][通常論文]
  • Hashimoto M, Sano H, Yoshida Y, Hori M, Kaga K, Oguchi H, Pashley DH. : Effects of multiple adhesive coatings on dentin bonding.
    Oper Dent 29(4) : 416-423 2004年 [査読無し][通常論文]
  • Ikeda T, Murata Y, Sano H. : Translucency of opaque―shade resin composites.
    Am J Dent. 17(2) : 127-30 2004年 [査読無し][通常論文]
     
    2004 Apr
  • Eiji Yoshida, Masanori Hashimoto, Minoru Hori, Masayuki Kaga, Hidehiko Sano, Haruhisa Oguchi. : Deproteinizing effects on resin-tooth bond structures.
    J Biomed Mater Res 68B(1) : 29-35 2004年 [査読無し][通常論文]
  • Color differences and color changes in Vita Shade tooth-colored restorative materials
    T Ikeda, A Nakanishi, T Yamamoto, H Sano AMERICAN JOURNAL OF DENTISTRY 16 (6) 381 -384 2003年12月 [査読無し][通常論文]
     
    Purpose: To evaluate the color differences of four tooth-colored restorative materials of the same designated Vita shade, and the color changes of the materials caused by light curing. Methods: CIELAB coordinates (L* a* b*) of four tooth-colored materials: Clearfil ST (CL), Beautifil (BE), Reactmer (RE) and Z250 (Z2) were determined in three shades (A2, A3 and B3) before and after light curing using a colorimeter. From the L* a* b* after light curing, CIELAB color differences (DeltaE*) between different cured materials of the same shade name were calculated. If the calculated DeltaE* were above 2, color differences were estimated as "perceptible". DeltaE* was also calculated before and after light curing, using the CIELAB values, in order to evaluate color changes caused by light curing. Results: As for the DeltaE* between various cured materials of the same shade, except for DeltaE* between BE and RE on A2 and between BE and RE on A3, every combination indicated perceptible color differences. The DeltaE* calculated before and after light curing of A2, A3 and B3 shades were 7.4, 7.1 and 7.2 for CL, 6.0, 5.1 and 4.8 for BE, 4.0, 3.2 and 3.4 for RE and 1.8, 1.7 and 2.1 for Z2, respectively, indicating that most materials showed perceptible color changes.
  • M Hashimoto, H Ohno, H Sano, M Kaga, H Oguchi BIOMATERIALS 24 (21) 3795 -3803 2003年09月 [査読無し][通常論文]
     
    Our knowledge of the mechanisms responsible for the degradation of resin-dentin bonds are poorly understood. This study investigated the degradation of resin-dentin bonds after 1 year immersion in water. Resin-dentin beams (adhesive area: 0.9 mm(2)) were made by bonding using a resin adhesive, to extracted human teeth. The experimental beams were stored in water for I year. Beams that had been stored in water for 24 h were used as controls. After water storage, the beams were subjected to microtensile bond testing. The dentin side of the fractured surface was observed using FE-SEM. Subsequently, these fractured beams were embedded in epoxy resin and examined by TEM. The bond strength of the control specimens (40.3 +/- 15.1 MPa) decreased significantly (p < 0.01) after 1 year of water exposure (13.3 +/- 5.6 MPa). Loss of resin was observed within fractured hybrid layers in the I year specimens but not in the controls. Transmission electron microscopic examination revealed the presence of micromorphological alterations in the collagen fibrils after I year of water storage. These micromorphological changes (resin elution and alteration of the collagen fibrils) seem to be responsible for the bond degradation leading to bond strength reduction. (C) 2003 Elsevier Science Ltd. All rights reserved.
  • J Konishi, F Watari, C Kawamoto, H Sano JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 66B (2) 553 -558 2003年08月 [査読無し][通常論文]
     
    The effect of the sphered particles on the contraction ratio of porcelain inlay processed by the cold isostatic pressure (CIP) method was investigated. The conventional lathe-cut porcelain powder was crushed to finer particles and the secondary particles with spherical shape by adding binders of acrylic resin, wax, and polyvinyl alcohol, respectively. Porcelain powder was molded as a disc-shaped green body in a refractory model and compressed at 200 MPa by CIP. From this green compact, the sintered porcelain was obtained by only one step of firing. The porcelain discs were then used for the measurements of contraction ratio, scanning microscopic observation, biaxial flexure strength, Vickers hardness, and density. Firing contraction was decreased to about 1% in the sphered particle groups, compared with 7% of the lathe-cut porcelain powder. Although biaxial flexure strength was about 85 MPa, which is lower than the 120 MPa of the control group, and the density was significantly decreased by about 10% from the 2.4 g/cm(3) of the control substance, Vickers hardness, which ranged from 531 to 537, showed no significant differences among all of the groups. The CIP method could save labor in the process of making porcelain inlays, and sphered powders could contribute significantly to a decrease in the contraction ratio in the sintering process. (C) 2003 Wiley Periodicals, Inc.
  • M Yamauti, M Hashimoto, H Sano, H Ohno, RM Carvalho, M Kaga, J Tagami, H Oguchi, M Kubota DENTAL MATERIALS 19 (5) 399 -405 2003年07月 [査読無し][通常論文]
     
    Objectives. Several recent studies have reported collagen hydrolysis within bonds over the long-term. This may be one reason for the degradation of the bonds. This study therefore aimed to determine the effects of NaOCl on adhesive system bonds (total-etch bonding system vs. self-etching primer system) to dentin in order to accelerate the durability testing. Methods. Resin-dentin bonded specimens were produced using Liner Bond 2V (Kuraray), a self-etching primer system, and OptiBond SOLO (Kerr), a total-etch bonding system, according to the manufacturers' instructions. The bonded specimens were serially sectioned in both x and y directions across the adhesive interface to obtain beams (adhesive area: 0.9 mm(2)). The specimens were immersed in 10% NaOCl solution for 1-5 h after being stored in water at 37 degreesC for 24 h. Control specimens were tested without exposure to NaOCl. After storage, micro-tensile bond tests were performed. Results were analyzed by two-way ANOVA and Fisher's PLSD tests (p < 0.05). All fractured surfaces were observed by SEM, and examined using an image analyzer. Results. The bond strengths decreased with increasing storage time in NaOCl. Fractography showed that NaOCl had a greater effect on the bond structure of OptiBond SOLO than on that of Liner Bond 2V, although both adhesives were susceptible. Significance. Deterioration of the bonds was responsible for the effect of NaOCl on the hybrid layer. This deterioration may occur in humans in cases of deproteinization within the bonds. (C) 2003 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.
  • Mechanical properties of adhesive resins at 5 minutes after light-curing.
    T. Ikeda, A. Takahashi, T. Tanaka, S. Inoue, H. Sano JOURNAL OF DENTAL RESEARCH 82 B335 -B335 2003年06月 [査読無し][通常論文]
  • Bonding efficacy of an all-in-one adhesive containing 4-MET(A)/UDMA.
    T. Abo, S. Uno, H. Sano JOURNAL OF DENTAL RESEARCH 82 B34 -B34 2003年06月 [査読無し][通常論文]
  • Is C-factor a reliable predictor of contraction stress?.
    T. Kuroe, K. Tachibana, H. Hasegawa, Y. Tanino, N. Satoh, N. Ohata, H. Sano, N. Inoue JOURNAL OF DENTAL RESEARCH 82 B58 -B58 2003年06月 [査読無し][通常論文]
  • Degradation patterns of different adhesives and bonding procedures.
    M. Kaga, M. Hashimoto, H. Ohno, H. Sano, H. Oguchi, D. H. Pashley JOURNAL OF DENTAL RESEARCH 82 B332 -B332 2003年06月 [査読無し][通常論文]
  • Fluoride uptake by enamel from calcium-containing glass-ionomer sealants.
    H. Komatsu, Y. Kobayashi, M. Noda, T. Ikeda, S. Inoue, H. Sano JOURNAL OF DENTAL RESEARCH 82 B259 -B259 2003年06月 [査読無し][通常論文]
  • Resin-enamel bonds made with self-etching primers.
    M. Hashimoto, H. Ohno, H. Sano, E. Yoshida, M. Hori, M. Kaga, H. Oguchi, D. H. Pashley JOURNAL OF DENTAL RESEARCH 82 B35 -B35 2003年06月 [査読無し][通常論文]
  • Consecutive coating for dentin bonding to reduce nanoleakage.
    H. Sano, M. Hashimoto, E. Yoshida, M. Hori, M. Kaga, H. Oguchi JOURNAL OF DENTAL RESEARCH 82 B101 -B101 2003年06月 [査読無し][通常論文]
  • In vivo long-term durability of adhesives bonded to monkey dentin.
    K. Koshiro, S. Inoue, T. Tanaka, K. Koase, M. Hujita, M. Hashimoto, J. De Munck, B. Van Meerbeek, H. Sano JOURNAL OF DENTAL RESEARCH 82 B126 -B126 2003年06月 [査読無し][通常論文]
  • R Fukuda, Y Yoshida, Y Nakayama, M Okazaki, S Inoue, H Sano, K Suzuki, H Shintani, B Van Meerbeek BIOMATERIALS 24 (11) 1861 -1867 2003年05月 [査読無し][通常論文]
     
    Previously, we introduced a methodology to determine the chemical bonding potential of polyalkenoic acids to mineralized tissues through quantification of the degree of ionic bond formation between the carboxyl groups of a polyalkenoic acid with calcium of hydroxyapatite. In a continuation of that study, we now investigated in how far the chemical bonding potential to synthetic hydroxyapatite is influenced by the molecular structure of the polyalkenoic acid and if this also may affect the self-adhesiveness to enamel and dentin. X-ray photoelectron spectroscopy and inductively coupled plasma atomic emission spectrometry were used to quantitatively analyze the chemical bonding efficacy of a polyalkenoic acid consisting of acrylic acid units (PAA) to synthetic hydroxyapatite (HAp) in comparison to the chemical bonding potential of the previously investigated synthesized polyalkenoic acid (s-PA) co-polymer consisting of 90 w/w% acrylic and 10 w/w% maleic acid units. In addition, the analysis was carried out for enamel and dentin samples. PAA revealed a significantly lower bonding effectiveness with only half of its carboxyl groups bonded to HAp versus about two-third of the carboxyl groups of s-PA. The difference in bonding potential was confirmed by the considerably lower adhesiveness of PAA to enamel and dentin as compared to that of s-PA The present findings indicate that the molecular structure of the polyalkenoic acid significantly influences the chemical bonding efficacy to Hap-based substrates. (C) 2003 Published by Elsevier Science Ltd.
  • Effect of depth and tubule direction on ultimate tensile strength of human coronal dentin
    S Inoue, PNR Pereira, C Kawamoto, M Nakajima, K Koshiro, J Tagami, RM Carvalho, DH Pashley, H Sano DENTAL MATERIALS JOURNAL 22 (1) 39 -47 2003年03月 [査読無し][通常論文]
     
    The purpose of this study was to evaluate the effect of dentin depth and tubule direction on the ultimate tensile strength (UTS) of human dentin. Dentin slabs of 0.5-mm thickness were trimmed either from the mesial and distal (for specimens with the tubules parallel to the tensile force; parallel group) or from the occlusal and pulpal surfaces (perpendicular group) to reduce the cross-sectional area of the superficial, middle, and deep regions to 0.25 mm(2), and subjected to microtensile testing. From SEM photomicrographs of the fractured specimens of the parallel group, the tubule density was investigated. For both parallel and perpendicular groups, superficial dentin showed a significantly higher UTS than deep dentin. The tubule density of superficial dentin was significantly lower than that of middle and deep dentin. When performing the microtensile bond test to deep dentin, it is possible that cohesive failure of dentin can occur at relatively low tensile stresses.
  • Inoue S, Vargas MA, Abe Y, Yoshida Y, Lambrechts P, Vanherle G, Sano H, Van Meerbeek B. : Microtensile bond strength of eleven contemporary adhesives to enamel.
    Am J Dent 16(5) : 329-34 2003年 [査読無し][通常論文]
     
    2003 Oct
  • Akiko Takahashi, Fumihiro Higashino, Mariko Aoyagi, Koichi Yoshida, Miyuki Itoh, Satoru Kyo, Takatoshi Ohno, Takahiro Taira, Hiryoyoshi Ariga, Kohichi Nakajima, Mitsutoki Hatta, Masanobu Kobayashi, Hidehiko Sano, Takao Kohgo, and Masanobu Shindoh. : EW・・・
    63 : 8338-8344 2003年 [査読無し][通常論文]
     
    Akiko Takahashi, Fumihiro Higashino, Mariko Aoyagi, Koichi Yoshida, Miyuki Itoh, Satoru Kyo, Takatoshi Ohno, Takahiro Taira, Hiryoyoshi Ariga, Kohichi Nakajima, Mitsutoki Hatta, Masanobu Kobayashi, Hidehiko Sano, Takao Kohgo, and Masanobu Shindoh. : EWS/ETS Fusions Activate Telomerase in Ewing’s Tumors Cancer Res 2003
  • Hashimoto M, Ohno H, Yoshida E, Hori M, Sano H, Kaga M, Oguchi H. : Resin-enamel bonds made with self-etching primers on ground enamel.
    Eur J Oral Sci 111(5) : 447-53 2003年 [査読無し][通常論文]
     
    2003 Oct
  • Mariko Aoyagi, Fumihiro Higashino, Motoaki Yasuda, Akiko Takahashi, Yukiharu Saswada, Yasunori Totsuka, Takako Kohgo, Hidehiko Sano, Masanobu Kobayashi, Masanobu Shindoh. : Nuclear export of adenovirus E4orf6 protein is necessary for its ability to ant・・・
    Oncogene 22, 6919-6927 2003年 [査読無し][通常論文]
     
    Mariko Aoyagi, Fumihiro Higashino, Motoaki Yasuda, Akiko Takahashi, Yukiharu Saswada, Yasunori Totsuka, Takako Kohgo, Hidehiko Sano, Masanobu Kobayashi, Masanobu Shindoh. : Nuclear export of adenovirus E4orf6 protein is necessary for its ability to antagonize apoptotic activity of BH3-only proteins.
  • Masanori Hashimoto, Hiroki Ohno, Hidehiko Sano, Masayuki Kaga, Haruhisa Oguchi. : Degradation patterns of different adhesives and bonding procedures.
    J Biomed Mater Res 66B(1) 324-330 2003年 [査読無し][通常論文]
  • Masamori Hashimoto, Franklin R. Tay, Hiroki Ohno, Hidehiko Sano, Masayuki Kaga, Cincia Yiu, Hiroshi Kumagai, Yoshiyuki Kudou, Minoru Kubota, Haruhisa Oguchi. : SEM and TEM analysis of water degradation of human dentinal collagen.
    J Biomed Mater Res 66B(1) 287-298 2003年 [査読無し][通常論文]
  • Kuroe T, Tachibana K, Tanino Y, Satoh N, Ohata N, Sano H, Inoue N, Caputo AA : Contraction Stress of Composite Resin Build-up Procedures for Pulpless Molars
    J Adhes Dent No.5(1) : 71-77 2003年 [査読無し][通常論文]
  • Mamoru Noda, John C. Wataha, Petra E. Lockwood, Keith R. Volkmann, Masayuki, Kaga and Hidehiko Sano : Sublethal, 2-week exposures of dental material components alter TNF- secretion of THP-1 monocytes.
    Dent Mater 19(2), 101-105 2003年 [査読無し][通常論文]
  • Agee KL, Pashley EL, Itthagarun A, Sano H, Tay FR, Pashley DH. : Submicron hiati in acid-etched dentin are artifacts of desiccation.
    Dent Mater 19(1), 60-68 2003年 [査読無し][通常論文]
  • Long-term durability of hybridized dentin.
    Sano, H, Inoue, S, Hashimoto, M, Takahashi, A, Noda, M, Koase, K, Kawamoto, C, Kubota, M Self-etching Primer; Current Status and Its Evolution 13 -22 2003年 [査読無し][通常論文]
  • 小西 順子, 川本 千春, 小松 久憲, 佐野 英彦, 亘理 文夫 日本歯科保存学雑誌 45 (6) 1112 -1121 2002年12月31日 [査読無し][通常論文]
  • M Noda, JC Wataha, PE Lockwood, KR Volkmann, M Kaga, H Sano JOURNAL OF BIOMEDICAL MATERIALS RESEARCH 62 (2) 237 -243 2002年11月 [査読無し][通常論文]
     
    The short-term degradation of dental polymers and alloys in biological environments has been well documented, but recent evidence indicates that oral tissues may be chronically exposed to low levels of these released components. The effect of these chronic exposures on the ability of cells to respond to a subsequent challenge is not known. To investigate this idea, we exposed human THP-1 monocytes to sublethal concentrations of HEMA, TEGDMA, Hg2+, and Ni2+ for 2 weeks and then assessed the monocytic response to subsequent 24-h challenge with the same components at higher concentrations. Chronic (2 week) exposures of monocytes to HEMA and both metal ions significantly altered monocyte response to short-term (24 h) secondary exposures, even when overt effects of the chronic exposures were not apparent. However, cellular responses were highly variable depending on the material and its concentrations. For TEGDMA, no effects were seen. These results demonstrate that the chronic effects of materials must be considered even when the chronic exposure has no initial overt effect. The effect on cells may only be apparent if the cell is challenged by a secondary exposure. (C) 2002 Wiley Periodicals, Inc.
  • Components of dentinal adhesives modulate heat shock protein 72 expression in heat-stressed THP-1 human monocytes at sublethal concentrations
    M Noda, JC Wataha, M Kaga, PE Lockwood, KR Volkmann, H Sano JOURNAL OF DENTAL RESEARCH 81 (4) 265 -269 2002年04月 [査読無し][通常論文]
     
    Few studies have investigated the ability of dental resins to induce cellular stress at sublethal concentrations. Cellular stress, especially in immune cells such as monocytes, may modulate the biological response to materials or the host's ability to respond to bacterially mediated inflammation. The current study examined the ability of sublethal concentrations of 2-hydroxylethylmethacrylate (HEMA) and triethyleneglycol dimethacrylate (TEGDMA) to induce heat shock protein 72 (HSP72) in human monocytes. HEMA and TEGDMA significantly suppressed heat-induced HSP72 expression, even at sublethal levels, but did not induce HSP72 by themselves. The results of the current study suggest that components released from dental resin could modulate the HSP stress response without altering cellular metabolic activity.
  • Bond strength of adhesives to enamel prepared with different burs
    F Sevgican, K Koase, C Kawamato, T Ikeda, S Inoue, H Sano JOURNAL OF DENTAL RESEARCH 81 A35 -A35 2002年03月 [査読無し][通常論文]
  • Effect of dental monomers on glutathione levels in monocytes.
    M Noda, JC Wataha, K Volkmann, M Kaga, P Lockwood, H Sano JOURNAL OF DENTAL RESEARCH 81 A427 -A427 2002年03月 [査読無し][通常論文]
  • Micromorphological changes in resin-dentin bonds after one-year water storage.
    M Hashimoto, H Ohno, H Sano, FR Tay, M Kaga, Y Kudou, Y Araki, M Kubota JOURNAL OF DENTAL RESEARCH 81 A139 -A139 2002年03月 [査読無し][通常論文]
  • Improved contraction of porcelain inlays by CIP method.
    C Kawamoto, J Konishi, F Watari, H Sano JOURNAL OF DENTAL RESEARCH 81 A251 -A251 2002年03月 [査読無し][通常論文]
  • Marginal leakage of cervical cavities treated with two-step adhesive systems.
    S Uno, T Abo, T Tanaka, H Sano JOURNAL OF DENTAL RESEARCH 81 A389 -A389 2002年03月 [査読無し][通常論文]
  • Microhardness of resin composites cured with an intensity-changeable light source.
    T Abo, S Uno, T Tanaka, H Sano JOURNAL OF DENTAL RESEARCH 81 A330 -A330 2002年03月 [査読無し][通常論文]
  • Over-etching effects on micro-tensile bond strength and failure patterns for two dentin bonding systems
    A Hashimoto, H Ohno, M Kaga, H Sano, FR Tay, H Oguchi, Y Araki, M Kubota JOURNAL OF DENTISTRY 30 (2-3) 99 -105 2002年02月 [査読無し][通常論文]
     
    Objectives. The purpose of this study was to determine (1) the weakest zone of resin-dentin bonds and (2) the relation between bond strength and failure mode to clarify the effect of demineralized dentin. Methods. Human premolars were sectioned to expose the dentin surfaces, and the dentin surfaces were conditioned with phosphoric acid for 15, 60, 120, or 180 s. Resin-dentin bonded specimens were produced using two adhesives: One-Step (Bisco) and OptiBond Solo (Kerr). Each sample was sectioned to produce a beam (adhesive area: 0.9 mm(2)). Microtensile bond tests were then conducted, and the mean bond strengths (n = 12 for each group) were statistically compared using two-way ANOVA and Duncan's multiple-range test (p < 0.05). The fractured surfaces of all specimens were examined using SEM, and the areas of failure were measured using an image analyzer. Results. For One-Step, the bond strength decreased with increase in acid-conditioning time (15 s: 50.7 +/- 9.7, 60 s: 40.8 +/- 11.0, 120 s: 23.6 +/- 4.9 and 180 s: 12.1 +/- 4.6 MPa) (p < 0.05). For OptiBond Solo, the bond strength in the case of 15 s acid-conditioning time (42.6 +/- 7.9 MPa) was significantly greater than that for the other times (60 s: 31.9 +/- 10.3, 120 s: 31.8 +/- 14.4 and 180 s: 31.8 +/- 7.4 MPa) (p < 0.05). Fractography showed that the area percentage of the hybrid layer increased with increase in etching time for both systems. Conclusions. The integrity of the hybrid layer, especially the top part, has an effect on bond strength. (C) 2002 Published by Elsevier Science Ltd.
  • Satoshi INOUE, Yasuhiro MURATA, Hidehiko SANO and Toshiaki KASHIWADA. : Effect of NaOCl Treatment on Bond Strength between Indirect Resin Core-buildup and Dentin.
    Dent Mater J 21(4) : 343-354 2002年 [査読無し][通常論文]
  • Hashimoto M, Ohno H, Sano H, Tay FR, Kaga M, Kudou Y, Oguchi H, Araki Y, Kubota M. : An analysis of long-term durability of resin dentin bonds using micromorphological and spectroscopic approach.
    In Adhesive Forum in Okayama in 2001. Edited by M Yoshiyama, Y Nishitani, S Nakabo, CF Cox. Pp 57-67 2002年 [査読無し][通常論文]
  • Takahashi A, Inoue S, Kawamoto C, Ominato R, Tanaka T, Sato Y, Pereira PNR, Sano H. : In vivo long-term durability of the bond to dentin using two adhesive systems.
    J Adhes Dent 4(2) : 151-159 2002年 [査読無し][通常論文]
  • Ikeda T, Uno S, Tanaka, T, Kawakami S, Komatsu H, Sano H. : Relation of enamel prism orientation to microtensile bond strength.
    Am J Dent. 15(2) : 109-113 2002年 [査読無し][通常論文]
  • Hashimoto M, Ohno H, Sano H, Tay FR, Kaga M, Kudou Y, Oguchi H, Araki Y, Kubota M. Micromorphological Changes in Resin-Dentin Bonds After One-Year Water Storage.
    J Biomed Mater Res (Appl Biomater) 63(3) : 306-311 2002年 [査読無し][通常論文]
  • The extent to which resin can infiltrate dentin by acetone-based adhesives
    M Hashimoto, H Ohno, M Kaga, H Sano, K Endo, H Oguchi JOURNAL OF DENTAL RESEARCH 81 (1) 74 -78 2002年01月 [査読無し][通常論文]
     
    The combined methodologies of fractography and laser-Raman spectroscopic analysis were used for evaluation of the resin-dentin bonds made with wet and dry bonding. Resin-dentin-bonded beams were produced by means of 2 acetone-based adhesives (One-Step and Prime & Bond NT). The microtensile bond test was conducted, and the fractured surfaces of all specimens were examined by SEM and an image analyzer. The amount of resin infiltration within the hybrid layer was quantified by means of a laser-Raman spectroscope. In Raman analysis, the amount of resin impregnation within the hybrid layer of the dry bonding was found to be significantly lower (approximately 50%) than that in the wet one. Under fractographic analysis, a correlation was found between the bond strength and the failure mode. Based on those findings, it was suggested that the integrity between the bonding resin and the top of the hybrid layer played a major role in bond strength.
  • Takahashi A, Sato Y, Uno S, Pereira PNR, Sano H. : Effects of mechanical properties of adhesive resins on bond strength to dentin.
    Dental Materials 18(3) 263-268 2002年 [査読無し][通常論文]
  • Hashimoto M, Ohno H, Kaga M, Sano H, Endo K, Oguchi H. : Fractured surface characterization: Wet vs Dry Bonding.
    Dent Mater 18(2) : 95-102 2002年 [査読無し][通常論文]
  • Effect of conditioner on the micro-tensile bond strength of a glass-ionomer adhesive.
    S Inoue, B Van Meerbeek, Y Abe, P Lambrechts, G Vanherle, H Sano JOURNAL OF DENTAL RESEARCH 80 (4) 1268 -1268 2001年04月 [査読無し][通常論文]
  • TEM and STEM/EDX study of an all-in-one adhesive containing Pre-Reacted Glass Ionomer fillers
    FR Tay, H Sano, J Tagami, M Hashimoto, KM Moulding, DH Pashley JOURNAL OF DENTAL RESEARCH 80 (4) 1375 -1375 2001年04月 [査読無し][通常論文]
  • Micro-tensile bond strength of two adhesive systems to cervical dentin.
    S Uno, WJ Finger, H Inoue, S Inoue, H Sano JOURNAL OF DENTAL RESEARCH 80 (4) 1206 -1206 2001年04月 [査読無し][通常論文]
  • Kaga M, Hashimoto M, Oguchi H, Sano H. : Approach to prevent progression of proximal caries originating in premature first molors.
    “Caries Preventive Restoration” Forum in Sapporo ‘99. Edited by Hisanori Komatsu, Satoshi Inoue, Hidehiko Sano. GC Corporation, Tokyo, Japan. 151-161 2001年 [査読無し][通常論文]
  • Takahashi A, Noda M, Sano H. : Degradation factors of dental polycarbonates.
    “Caries Preventive Restoration” Forum in Sapporo ‘99. Edited by Hisanori Komatsu, Satoshi Inoue, Hidehiko Sano. GC Corporation, Tokyo, Japan. 145-150 2001年 [査読無し][通常論文]
  • Hashimoto M, Ohno H, Kaga M, Sano H, Endo K, Oguchi H. : Analysis of the weakest zone within resin-dentin bond structures.
    “Caries Preventive Restoration” Forum in Sapporo ‘99. Edited by Hisanori Komatsu, Satoshi Inoue, Hidehiko Sano. GC Corporation, Tokyo, Japan. 133-144 2001年 [査読無し][通常論文]
  • Inoue H, Inoue S, Uno S, Komatsu M, Sano H. : Micro-tensile bond strength of two self-etching priming adhesive systems to occlusal and buccal dentin.
    “Caries Preventive Restoration” Forum in Sapporo ‘99. Edited by Hisanori Komatsu, Satoshi Inoue, Hidehiko Sano. GC Corporation, Tokyo, Japan. 115-122 2001年 [査読無し][通常論文]
  • Okuyama K, Komatsu H, Sano H. : Remimeralization of root surface caries by Fluoride containing Materials.
    “Caries Preventive Restoration” Forum in Sapporo ‘99. Edited by Hisanori Komatsu, Satoshi Inoue, Hidehiko Sano. GC Corporation, Tokyo, Japan. 57-67 2001年 [査読無し][通常論文]
  • Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. : Fractographical analysis of resin-dentin bonds.
    American Journal of Dentistry 14(6) : 355-360 2001年 [査読無し][通常論文]
  • Uno S, Inoue H, Finger WJ, Inoue S, Sano H. : Miro-tensile bond strength of three adhesive systems in cervical dentin cavities.
    Journal of Adhesive Dentistry 3(4) : 3233-341 2001年 [査読無し][通常論文]
  • Tay FR, Sano H, Tagami J, Hashimoto M, Moulding KM, Yiu C, Pashley DH. : Ultrastructural study of a glass ionomer-based all-in one adhesive.
    Journal of Dentistry 29(7) : 489-498 2001年 [査読無し][通常論文]
  • Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. : Resin-tooth adhesive interfaces after long-term function.
    Am J Dent 14(4) : 211-215 2001年 [査読無し][通常論文]
  • Inoue S, Vargas MA, Van Meerbeek B, Abe Y, Yoshida Y, Yoshida Y, Lambrechts P, Vanherle G, Sano H. : Micro-tensile bond strength of eleven modern adhesives to dentin.
    Journal of Adhesive Dentistry 3(3) 237-245 2001年 [査読無し][通常論文]
  • Inoue H, Inoue S, Uno S, Takahashi A, Koase K, Sano H. : Micro-tensile bond strength of two single step adhesive systems.
    Journal of Adhesive Dentistry 3(2), 129-136 2001年 [査読無し][通常論文]
  • Pereira PNR, Okuda M, Nakajima M, Sano H, J Tagami, Pashley DH. : Relationship between bond strength and nanoleakage: evaluation of a new assessment method.
    Am J Dent 14(2), 100-104 2001年 [査読無し][通常論文]
  • Tay FR, Pashley EL, Huang C, Hashimoto M, Sano H, Samles RJ, Pashley DH. : The glass ionomer phase in resin-based restorative materials.
    J Dent Res 80(9) : 1808-1812 2001年 [査読無し][通常論文]
  • Inoue S, Van Meerbeek B, Abe Y, Yoshida Y, Lambrechts P, Vanherle G, Sano H. : Effect of remaining dentin thickness and the use of conditioner on micro-tensile bond strenght of a glass-ionomer adhesive.
    Dent Mater 17(5) 445-455 2001年 [査読無し][通常論文]
  • Kaga M, Noda M, Ferracane JL, Nakakura W, Oguchi H, Sano H. : The in vitro cytotoxicity of eluates from dentin bonding resins and their effect on tryosine phosphorylation of L929 cells.
    Dental Materials 17(4), 333-339 2001年 [査読無し][通常論文]
  • Ogata M, Nakajima M, PNR Pereira, Sano H, Tagami J. : Influence of the direction of tubules on bond strength to dentin.
    Oper Dent 27(1) : 27-35 2001年 [査読無し][通常論文]
  • Zheng L, Pereira PNR, Nakajima M, Sano H, Tagami J. : Relationship between adhesive thickness and microtensile bond strength.
    Operative Dentistry 26(1) 97-104 2001年 [査読無し][通常論文]
  • The effect of hybrid layer thickness on bond strength: demineralized dentin zone of the hybrid layer
    M Hashimoto, H Ohno, K Endo, M Kaga, H Sano, H Oguchi DENTAL MATERIALS 16 (6) 406 -411 2000年11月 [査読無し][通常論文]
     
    Objectives: The purpose of this study was to evaluate the correlation between hybrid layer thickness and bond strength using specimens acid-conditioned for varying lengths of time. Methods. The dentin surfaces of human premolars, sectioned to remove the enamel from the labial surface, were conditioned with 35.0% phosphoric acid of an adhesive resin system (Scotchbond Multi-Purpose; 3M) for 15 (as directed by the manufacturer), 60, 120, or 180 s (experimental acid-conditioning times). The bonded specimens were then sectioned perpendicular to the adhesive interface to measure the hybrid layer thickness by SEM. The specimens for the micro-tensile test were sectioned perpendicular to the adhesive interface and trimmed to an hourglass-shape. Then, the micro-tensile test was performed at a crosshead speed of 1.0 mm/min. The bond strengths and hybrid layer thickness were statistically compared with Student's t-test (p < 0.05). All fractured surfaces were also observed by SEM. Results: Significant differences between the groups exposed to acid for 15 and 60 s, and those exposed for 120 and 180 s were observed in hybrid layer thickness and bond strength (p < 0.05). SEM observation of the fractured surfaces revealed that a demineralized dentin zone without resin impregnation remained within the hybrid layer. Significance A demineralized dentin zone was formed in the bond structures after prolonged acid-conditioning, resulting in low bond strength. The shrinkage of the hybrid layer due to desiccation during the SEM examination process provided evidence of the presence of the demineralized dentin zone within the hybrid layer. (C) 2000 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.
  • Effect of region and dentin perfusion on bond strengths of resin-modified glass ionomer cements
    PNR Pereira, H Sano, M Ogata, L Zheng, M Nakajima, J Tagami, DH Pashley JOURNAL OF DENTISTRY 28 (5) 347 -354 2000年07月 [査読無し][通常論文]
     
    Objectives: The purpose of this study was to evaluate the hypothesis that regional differences and pulpal pressure would significantly affect bond strengths of resin-modified glass ionomer cements to dentin. Methods: Twenty-six extracted caries-free human third molars were ground to expose middle dentin and were randomly divided into two groups for bonding: no pulpal pressure and pulpal pressure of 15 cm H2O. Fuji II LC, Vitremer, or Photac-Fil Quick were applied to the previously pre-treated surfaces and light-cured as recommended by the manufacturers. After immersion in water at 37 degrees C for 24 h, the teeth were sectioned to 0.7 mm thick slabs, divided into pulp horn, center, and peripheral regions according to visual criteria, and trimmed along the bonded interface for microtensile bond test to a cross-sectional area of 1 mm(2). The slabs were subjected to tensile forces and the data analyzed using ANOVA and Fisher's PLSD at the 95% level of confidence. Results: For Fuji II LC, bond strengths to the pulp horn regions were significantly lower than those to the other regions independent of pulpal pressure (p < 0.05). On the other hand, regional bond strengths were not observed for Vitremer and Photac-fil Quick (p > 0.05). Conclusions: Pulpal pressure had a stronger influence on bond strengths and failure modes of resin-modified glass ionomers than regional differences of the substrate. (C) 2000 Elsevier Science Ltd. All rights reserved.
  • In vivo degradation of resin-dentin bonds in humans over 1 to 3 years
    M Hashimoto, H Ohno, M Kaga, K Endo, H Sano, H Oguchi JOURNAL OF DENTAL RESEARCH 79 (6) 1385 -1391 2000年06月 [査読無し][通常論文]
     
    The longevity of resin restorations is currently an area of great interest in adhesive dentistry. However, no work has been conducted to investigate the durability of resin-dentin bond structures using human substrate in vivo. The purpose of this study was to investigate the degradation of the resin-dentin bond structures aged in an oral environment for 1, 2, or 3 years. Cavities were prepared in primary molars, and an adhesive resin system (Scotchbond Multi-Purpose) was applied to the cavity. After 1 to 3 years, following the eruption of the succedaneous permanent teeth, the resin-restored teeth were extracted. Immediately after extraction, those teeth were sectioned perpendicular to the adhesive interface and trimmed to produce an hourglass-shaped specimen. Then, a micro-tensile test was performed at a crosshead speed of 1.0 mm/min. The mean bond strengths were statistically compared with one-way ANOVA and Fisher's PLSD test (p < 0.05). Further, all fractured surfaces were observed by SEM, and the area fraction of failure mode was calculated by means of a digital analyzer on SEM photomicrographs. There were significant differences in tensile-bond strength among all 3 groups (p < 0.05), with mean values ranging from 28.3 +/- 11.3 MPa (control), to 15.2 +/- 4.4 MPa (1 to 2 years), to 9.1 +/- 5.1 MPa (2 to 3 years). Moreover, under fractographic analysis, the proportion of demineralized dentin at the fractured surface in specimens aged in an oral environment was greater than that in control specimens. Furthermore, degradation of resin composite and the depletion of collagen fibrils was observed among the specimens aged in an oral environment. Analysis of the results of this study indicated that the degradation of resin-dentin bond structures occurs after aging in the oral cavity.
  • Microtensile bond strength to dentin and cavity adaptation of Cerec 2 inlay restoration
    S Uno, T Tanaka, C Kawamoto, J Konishi, H Sano AMERICAN JOURNAL OF DENTISTRY 13 (2) 59 -63 2000年04月 [査読無し][通常論文]
     
    Purpose: To examine the bond strength of Cerec 2 inlays both to dentin surface by microtensile testing and to cavity walls by cavity adaptation in Class II restorations, using three luting materials. Material and Methods: VitaMark II disc was bonded to the coronal dentin surface with Clapearl DC (Clearfil DC)/Linerbond II Sigma (Linerbond 2V), AP-X/Linerbond II Sigma or Fluorocement (Panavia F)/ED Primer. After 24-hr storage in water, microtensile bond strengths (MTB) were measured. Additionally, Cerec inlays were luted to the MO cavities prepared in molars with the same materials. After thermocycling (x2000), the restorations were sectioned mesio-distally. Gap formation was microscopically examined along the cavity walls. Results: There was no significant difference in MTB among the materials (Clapearl DC: 20.89 +/- 4.58 MPa, PLP-X: 24.22 +/- 5.97 MPa, Fluorocement: 19.82 +/- 6.43 MPa, Scheffe, P > 0.05). The frequency of gap formation was higher in AP-X than in Clapearl DC and Fluorocement (chi(2)-test, P < 0.05). Debonding occurred more often at the luting material/dentin interface than at the inlay/luting material interface (Sign test, P < 0.05).
  • Bonding to caries-affected dentin using self-etching primers (vol 12, pg 309, 1999)
    M Nakajima, M Ogata, M Okuda, J Tagami, H Sano, DH Pashley AMERICAN JOURNAL OF DENTISTRY 13 (2) 72 -72 2000年04月 [査読無し][通常論文]
  • Tay FR, SM Kwang, Sano H, Carlvalho R, Pashley EL, Pashley DH. : Sataus of research on new fillers and new resins for dental composites.
    In Advanced Adhesive Dentistry, Edited by J Tagami, Manuel Toredano, and Carlo Prati, printed in Cirimido, Italy, april 2000 pp105-129 2000年 [査読無し][通常論文]
  • Bouillague S, Duroux B, Ciucchi B, Sano H. : Ability of adhesive systems to seal dentin surfaces: An in vitro study.
    Journal of Adhesive Dentistry 2(3), 201-208 2000年 [査読無し][通常論文]
  • Uno S, Tanaka T, Sano H. : Elastic beheviors of the resin/dentin bonding interfaces.
    Modern trends in adhesive dentistry, Proceedings of the Adhesive Forum in 99 Sturumi, KurerayCo. Ltd, Osaka, First Edtion, Edited by Y Momoi, N Akimoto, A Kohno pp 97-107 2000年 [査読無し][通常論文]
  • H Sano, S Inoue, M Noda, Kawamoto C, Uno S, Takahashi A, Aoyagi M, Sato Y, Natsuizaka A. : Long-term Durability of Dentin Bonding.
    Modern trends in adhesive dentistry, Proceedings of the Adhesive Forum in 99 Sturumi, Kureray Co Ltd, Osaka, First Edtion, Edited by Y Momoi, N Akimoto, A Kohno pp 31-37 2000年 [査読無し][通常論文]
  • Urabe I, Nalajima M, Sano H, Tagami J. : Physical properties of the dentinoenamel junction region.
    Am J Dent 13(3), 129-135 2000年 [査読無し][通常論文]
  • Tay FR, Sano H, Carvalho RM, Pashley DH. : An ultrastructural study of the influence of acidity of self-etching primers and smear layer thickness on bonding to intact detnin
    Adhesive Dent 2(2), 83-98 2000年 [査読無し][通常論文]
  • Tay FR, Carvalho RM, Sano H, Pashley DH. : Effect of smear layers on the bonding
    2000年 [査読無し][通常論文]
  • Bond strengths of single-bottle dentin adhesives to caries-affected dentin
    M Nakajima, H Sano, Urabe, I, J Tagami, DH Pashley OPERATIVE DENTISTRY 25 (1) 2 -10 2000年01月 [査読無し][通常論文]
     
    There is concern that some acidic conditioners may not be strong enough to adequately etch sclerotic or caries-affected dentin. The hypothesis that was tested was that there were no significant differences in the bond strengths of single-bottle bonding systems to normal or caries-affected dentin, regardless of the strength of the phosphoric-acid conditioner. Extracted teeth with coronal caries extending into mid-dentin were prepared by grinding the occlusal surface flat. This left a central region of caries-affected dentin surrounded by normal dentin. The One-Step bonding system was used to bond dentin following etching with 10 or 32% phosphoric acid. The Single Bond system was used after etching dentin with 10 or 35% phosphoric acid. After 24 hours in water, serial vertical sections were made through the bonded teeth to create slabs 0.7 mm thick. Each tooth yielded four to five slabs, some of which included normal dentin, while others included caries-affected dentin. Each slab was trimmed into an hourglass configuration to limit the test area to normal or caries-affected dentin. The results obtained with One-Step following etching with 10% phosphoric acid showed lower (P < 0.05) tensile bond strengths to caries-affected dentin compared to normal dentin (36.9 +/- 8.0 MPa vs 47.7 +/- 6.5 MPa, respectively). This difference disappeared when using 32% phosphoric acid (49.7 +/- 6.1 MPa vs 45.0 +/- 7.2 MPa, respectively). Bonds made to caries-affected dentin with Single Bond were always lower than bonds to normal dentin regardless of the strength of the phosphoric acid. Scanning electron microscopy of polished cross sections sequentially challenged with acid and NaOCl revealed loss of the middle of the hybrid layers created by either bonding system in caries-affected dentin etched with 10% phosphoric acid. It is clear that 32-35% phosphoric acid is required to adequately etch caries-affected dentin in order to produce high bond strengths and well-infiltrated demineralized dentin.
  • Noda M, Komatsu H, Inoue S, Sano H : Antibiotic susceptibility of bacteria detected from the root canal exudate of persistant apical periodontitus.
    J Endodontics 26(4) : 221-224 2000年 [査読無し][通常論文]
  • Bleaching effect of sodium percarbonate on discolored pulpless teeth in vitro
    J Kaneko, S Inoue, S Kawakami, H Sano JOURNAL OF ENDODONTICS 26 (1) 25 -28 2000年01月 [査読無し][通常論文]
     
    The purpose of this study was to evaluate the bleaching effect of sodium percarbonate on artificially stained pulpless teeth. Twenty extracted human mandibular premolers were stained by immersing them in human blood mixed with iron sulfide, and divided into three test groups and one control group. The following bleaching agents were used: sodium perborate mixed with 30% hydrogen peroxide (WB), sodium percarbonate mixed with distilled water (PW) or 30% hydrogen peroxide (PH), On days 5, 10, and 15, each pulp chamber was refilled with fresh bleaching paste. The L* (average lightness) of the midbuccal area of specimens was measured before and after staining, and on days 5, 10, 15, and 20 by colorimeter. The bleaching effect was evaluated by the lightness recovery rate (LRR) calculated from L*. WB showed the highest LRR among all groups, and PW and PH showed significantly higher LRR when compared with the control group, PW and PH were not significantly different. Results showed that sodium percarbonate had an obvious bleaching effect without hydrogen peroxide and indicated that it could be a safe intracoronal bleaching agent.
  • Effect of fluoride-containing materials and fluoride-mouthrinse on caries progression.
    K Okuyama, H Komatsu, H Sano JOURNAL OF DENTAL RESEARCH 79 358 -358 2000年 [査読無し][通常論文]
  • An ultrastructural study of bonding in dentin smear layers.
    E Pashley, FR Tay, H Sano, RM Carvalho, DH Pashley JOURNAL OF DENTAL RESEARCH 79 268 -268 2000年 [査読無し][通常論文]
  • Fluoride release or fluoride containing materials with successive fluoride application.
    H Komatsu, K Okuyama, Y Konno, H Sano JOURNAL OF DENTAL RESEARCH 79 358 -358 2000年 [査読無し][通常論文]
  • Micro-tensile bond strength of an experimental resin-modified glass-ionomer adhesive.
    S Inoue, B Van Meerbeek, Y Abe, Y Yoshida, P Lambrechts, G Vanherle, H Sano JOURNAL OF DENTAL RESEARCH 79 597 -597 2000年 [査読無し][通常論文]
  • Effect of smear layers on bonding of a self-etching primer to dentin.
    FR Tay, H Sano, RM Carvalho, DH Pashley JOURNAL OF DENTAL RESEARCH 79 356 -356 2000年 [査読無し][通常論文]
  • Tensile properties at demineralized dentin matrix after 48 months.
    RM Carvalho, F Tay, H Sano, M Yoshiyama, DH Pashley JOURNAL OF DENTAL RESEARCH 79 249 -249 2000年 [査読無し][通常論文]
  • Effect of ultimate tensile strength of adhesives on Bond Strength.
    A Takahashi, Y Sato, C Kawamoto, M Hashimoto, S Uno, H Komatsu, H Sano JOURNAL OF DENTAL RESEARCH 79 508 -508 2000年 [査読無し][通常論文]
  • Relationship between the thickness of resin layer and bond strength
    L Zheng, PNR Pereira, M Nakajima, H Sano, J Tagami JOURNAL OF DENTAL RESEARCH 79 509 -509 2000年 [査読無し][通常論文]
  • Long-term Durability of Dentin Bonding.
    Sano, H, Inoue, S, Noda, M, Kawamoto, C, Uno, S, Takahashi, A, Aoyagi, M, Sato, Y, Natsuizaka, A Modern trends in adhesive dentistry 31 -37 2000年 [査読無し][通常論文]
  • Effect of moist vs. dry bonding to normal vs. caries-affected dentin with Scotchbond Multi-Purpose Plus
    M Nakajima, H Sano, L Zheng, J Tagami, D Pashley JOURNAL OF DENTAL RESEARCH 78 (7) 1298 -1303 1999年07月 [査読無し][通常論文]
     
    Recent research in dentin bonding demonstrated the superiority of moist bonding over dry bonding on normal dentin, but it is unclear if this technique is also superior in bonding to caries-affected dentin. The purpose of this study was to evaluate the SEM appearance and bond strength of Scotchbond Multi-Purpose Plus (SMPP) to normal vs. caries-affected dentin bonded under moist vs. dry conditions, with and without polyalkenoic acid in the primer. Extracted carious human third molars were ground down by means of 600-grit SiC paper until the carious dentin no longer stained with caries detector solution, The flat surfaces were then primed, bonded, and built up with resin composite. After soaking in water for 1 day, the teeth were serially sectioned vertically into 5 or 6 slabs 0.7 mm thick. The bonded caries-affected areas were isolated by means of an ultrafine diamond bur to create an hourglass configuration with a cross-sectional area of 0.9 mm(2). Bonded normal dentin was isolated the same way. Each specimen was attached to a Bencor device and tested in tension to failure. SMPP bonds to dry, normal dentin were only half as strong (21 +/- 10 MPa, (x) over bar +/- SD) as those made to moist, normal dentin (42 +/- 9 MPa, p < 0.01). There was no significant difference between bonds made to normal vs. caries-affected dentin by means of the moist technique (42 +/- 9 vs. 48 +/- 4 MPa, respectively). Removal of the polyalkenoic acid from the primer lowered (p < 0.05) the bond strength of SMPP to caries-affected dentin (38 +/- 8 MPa). The benefits of moist bonding extend to caries-affected dentin. The polyalkenoic acid in the SMPP primer contributes to the high bond strength that can be achieved ta caries-affected dentin.
  • Effects of dentin depth and cavity configuration on bond strength
    T Yoshikawa, H Sano, MF Burrow, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 78 (4) 898 -905 1999年04月 [査読無し][通常論文]
     
    During polymerization of resin composites, shrinkage stresses compete with resin-dentin bonds in a manner that can cause failure of the bond, depending upon the configuration of the cavity, its depth, and the restorative technique. The hypothesis tested in this study was that the effect of cavity configuration (C) and remaining dentin thickness (RDT) influence resin bond strength to the dentin of Class I cavity floors. The occlusal enamel was ground to expose a flat superficial dentin surface as a control (superficial dentin, C-factor = 1) in human extracted third molars. Cavities 3 mm long x 4 mm wide were prepared to a depth 2 mm below the ground dentin surfaces (deep dentin within cavity floor, C-factor = 3). To assess the relationship between C-factor and RDT, we removed the walls of cavities, making a deep flat surface for bonding (deep dentin, C-factor = I). The teeth were restored with either Clearfil Liner Bond II (LB II), One-Step (OS), or Super-Bond D Liner (DL), followed by Clearfil Photo Posterior resin composite. After 24 hrs' storage in water, the teeth were sectioned vertically into 3 or I slabs (0.7 mm thick) and trimmed for the micro-tensile bond test so that we could determine the strength of the resin bonds to the pulpal floor. All groups gave high bond strengths to superficial dentin, but OS and DL gave significantly lower bond strengths to flat deep dentin when the C-factor was 1. When the C-factor was increased to 3 by the creation of a three-dimensional cavity preparation, the bond strengths of ail materials fell (range, 21 to 35%), but the difference was significant (p < 0.05) only with DL. SEM observations of failure patterns showed that specimens with high bond strengths tended to exhibit cohesive failures within the hybrid layer, while specimens exhibiting low bond strengths showed failures at the tap of the hybrid layer. Some adhesives do not bond well to deep dentin, making them more susceptible to polymerization shrinkage stress that develops in cavities with high C-factors.
  • Long-term durability of dentin bonds made with a self-etching primer, in vivo
    H Sano, T Yoshikawa, PNR Pereira, N Kanemura, M Morigami, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 78 (4) 906 -911 1999年04月 [査読無し][通常論文]
     
    The long-term durability of bonds between adhesive resins and dentin is of significant importance for the longevity of bonded restorations. We carried out an in vivo one-year study to evaluate the durability of resin-dentin bends in the oral cavity, as well as to test the hypothesis that the adhesive interface would show morphological changes in cine over time. Very shallow saucer-shaped dentin cavities were prepared in 12 intact teeth of one Japanese monkey (Macaca fuscata) under general anesthesia. The cavities were restored with Clearfil Liner Bond II and Clearfil Photo Posterior resin composite. The teeth were extracted at three different times: immediately, and 180 and 360 days after placement of the restorations. One day after the monkey was killed, specimens of the three time periods were subjected to the micro-tensile bond test at a crosshead speed of I mm/min. The surfaces of the failed bonds were observed under a field emission scanning electron microscope (FE-SEM). Bond strength measurements in this study were successfully performed and were stable at approximately 19 MPa Juring the one-year testing. Scanning electron microscopic observations of the failed surfaces revealed, at the top of the hybrid layer and within the adhesive resin, porosity which increased over time. Long-term bonds can be assessed in vivo by the combined evaluation of the microtensile bond strength and SEM morphological examination of the adhesive interface.
  • Antibiotic susceptibility of bacteria detected from the root canal exudate of persistant apical periodontitis
    Journal of Endodontics 36 (4) 221 -224 1999年 [査読無し][通常論文]
  • Journal of Biomedical and Materials Research 47 (3) 374 -378 1999年 [査読無し][通常論文]
  • Shigeru UNO, Toru TANAKA, Satoshi INOUE, Hidehiko SANO Dental Materials Journals 18 (1) 19 -31 1999年 [査読無し][通常論文]
  • Adhesion of resin-modified glass ionomer cements using resin bonding systems
    PNR Pereira, T Yamada, S Inokoshi, MF Burrow, H Sano, J Tagami JOURNAL OF DENTISTRY 26 (5-6) 479 -485 1998年07月 [査読無し][通常論文]
     
    Objectives: To compare the in vitro shear bond strength of two commercially available resin-modified glass ionomer cements (RmGIC) to bovine dentine, with and without the use of adhesive bonding systems. Methods: Ninety-six flat bovine dentine surfaces were divided into eight groups for bonding procedures. Fuji II LC and Vitremer were bonded to dentine that had been treated with and without Clearfil Photo Bond, Clearfil Liner Bond, or Clearfil Liner Bond II. The control group consisted of specimens that were treated as recommended by the manufacturer of each resin-modified glass ionomer cement. The bonded assemblies were stored in tap water for 24 h at 37 degrees C, and shear bond strengths measured using a universal-testing machine at a crosshead speed of 1 mm/min. Results: For the control group, shear bond strength of Fuji II LC was significantly greater than Vitremer (P < 0.005). However, when any of the resin bonding systems was applied, no statistical difference between Fuji II LC and Vitremer was determined (P > 0.05). The Clearfil PhotoBond and Liner Bond groups showed similar bond strengths (P > 0.05), but were significantly less than Liner Bond II (P < 0.001). Conclusions: A recent bonding system combined with the RmGICs exhibited the greatest shear bond strength to dentine, irrespective of chemical differences that may exist between the two RmGICs. (C) 1998 Elsevier Science Ltd. All rights reserved.
  • Bonding to HMDS-dried or wet etched dentin.
    M Nakajima, H Sano, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 77 237 -237 1998年 [査読無し][通常論文]
  • Effect of dentin region and perfusion on RmGIC bond strength.
    PNR Pereira, H Sano, M Ogata, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 77 225 -225 1998年 [査読無し][通常論文]
  • Tensile bond strength to caries-affected dentin using self-etching primers.
    M Nakajima, H Sano, S Uno, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 77 638 -638 1998年 [査読無し][通常論文]
  • Correlation between bond strengths and nanoleakage.
    PNR Pereira, M Okuda, M Nakajima, H Sano, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 77 891 -891 1998年 [査読無し][通常論文]
  • Simplified fabrication method of porcelain inlay using cold isostatic press.
    C Kawamoto, F Watari, M Uo, H Komatsu, H Sano JOURNAL OF DENTAL RESEARCH 77 940 -940 1998年 [査読無し][通常論文]
  • Long term tensile properties of demineralized dentin matrix.
    RM Carvalho, H Sano, M Yoshiyama, DH Pashley JOURNAL OF DENTAL RESEARCH 77 168 -168 1998年 [査読無し][通常論文]
  • Hardness and tensile properties of the dentinoenamel junction.
    Urabe, I, H Sano, J Tagami JOURNAL OF DENTAL RESEARCH 77 821 -821 1998年 [査読無し][通常論文]
  • Long term durability of dentin bonding in vivo.
    H Sano, PNR Pereira, N Kanemura, M Morigami, T Yoshikawa, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 77 297 -297 1998年 [査読無し][通常論文]
  • Effect of multiple primer application on cervical dentin.
    M Ogata, M Okuda, M Nakajima, H Sano, J Tagami JOURNAL OF DENTAL RESEARCH 77 636 -636 1998年 [査読無し][通常論文]
  • The long-term durability of two bonding systems to dentine.
    N Harada, MF Burrow, Y Kitasako, H Sano, T Yamada, S Inokoshi, J Tagami JOURNAL OF DENTAL RESEARCH 76 (5) 934 -934 1997年05月 [査読無し][通常論文]
  • Tensile and shear bond strengths of four generations of resin bonding systems.
    T Yamada, SM Omomo, PNR Pereira, N Harada, H Sano, S Inokoshi, J Tagami JOURNAL OF DENTAL RESEARCH 76 (5) 1172 -1172 1997年05月 [査読無し][通常論文]
  • Effect of C-factor and depth on bond strength to dentin.
    T Yoshikawa, H Sano, S Inokoshi, T Yamada, J Tagami, B Ciucchi, DH Pashley JOURNAL OF DENTAL RESEARCH 76 201 -201 1997年 [査読無し][通常論文]
  • Calculation of stress zones during crack propagation.
    K Wakasa, Y Yoshida, B Ciucchi, H Sano, DH Pashley JOURNAL OF DENTAL RESEARCH 76 199 -199 1997年 [査読無し][通常論文]
  • Regional bond strength in cervical dog dentin in vivo.
    M Yoshiyama, M Nakajima, H Sano, T Matsuo, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 76 1378 -1378 1997年 [査読無し][通常論文]
  • Tensile bond strength to caries-affected dentin.
    M Nakajima, H Sano, J Tagami, DH Pashley JOURNAL OF DENTAL RESEARCH 76 3223 -3223 1997年 [査読無し][通常論文]
  • Bond strength to crown and root dentin
    MF Burrow, H Sano, M Nakajima, N Harada, J Tagami AMERICAN JOURNAL OF DENTISTRY 9 (5) 223 -229 1996年10月 [査読無し][通常論文]
     
    Purpose: To investigate (1) the tensile bond strengths of four commercial dentin bonding systems to bovine crown and root dentin and (2) the structure of the hybrid layers for each system bonded to the two dentin substrates. Materials and Methods: Superficial surfaces were exposed in bovine crown and root dentin. The teeth were embedded in plaster and a 3 mn diameter bonding area was demarcated. The four bonding systems used were All-Bond 2, Super-Bond D-Liner Plus, Clearfil Liner Bond LI, and ProBond. Bonding procedures followed the manufacturers' instructions with the exception of Super-Bond D-Liner Plus where the primer was left in situ for 60 seconds. Tensile bond strengths were tested after 24-hour storage in 37 degrees C deionized water. Specimens were also prepared for SEM observation of the hybrid layer, after treatment with 10% phosphoric acid, and 10% phosphoric acid and 5% sodium hypochlorite. Results: Statistically lower bond strengths to crown dentin when compared with root dentin were observed for All-Bond 2, whereas Liner Bond II showed the opposite (P<0.01). Both ProBond and Super-Bond D-Liner Plus showed no statistical differences between crown and root dentin (P>0.05). Hybrid layers could be observed for All-Bond 2, Liner Bond II and Super-Bond D-Liner Plus, with no apparent differences between the hybrid layers of crown and root dentin. In the case of ProBond, where the smear layer was not removed during the priming stage, it appeared that the primer had infiltrated and caused hybridization of the smear layer. The differences in bond strength were thought to be related to the different bonding mechanisms of each material, as well as possible variations in the crown and root dentin substrates.
  • Regional tensile bond strengths of cervical sclerotic root dentin.
    M Yoshiyama, H Sano, S Ebisu, R Carvalho, C Russell, DH Pashley JOURNAL OF DENTAL RESEARCH 75 (6) 2988 -2988 1996年 [査読無し][通常論文]
  • COMPARATIVE SEM AND TEM OBSERVATIONS OF NANOLEAKAGE WITHIN THE HYBRID LAYER
    H SANO, M YOSHIYAMA, S EBISU, MF BURROW, T TAKATSU, B CIUCCHI, R CARVALHO, DH PASHLEY OPERATIVE DENTISTRY 20 (4) 160 -167 1995年07月 [査読無し][通常論文]
     
    Most adhesive interface studies have involved SEM demonstration of the penetration of adhesive resins into demineralized dentin surfaces with subsequent creation of hybrid layers. Nanoleakage is a term that describes the diffusion of small ions or molecules within the hybrid layer in the absence of gap formation. The present microscopic study examined the nanoleakage of the hybrid layer using a silver nitrate staining technique. Adhesive dentin sandwiches, which were immersed in a silver nitrate solution, were prepared for both SEM and TEM examination using both the Clearfil Liner Bond and All-Bond 2 adhesive systems. systems demonstrated silver accumulation the hybrid layers. Clearfil Liner Bond System showed scattered silver particles at the bottom two-thirds of the hybrid layer by both SEM and TEM observation, whereas All-Bond 2 revealed stained fiber-like structures within the full thickness of the hybrid layer. To evaluate the quality of the hybrid layer, the utilization of tracer molecules such as silver nitrate that are detectable by both SEM and TEM is proposed. It is important to determine the location and morphology of these nanometer-sized porosities that may permit the hydrolysis of collagen fibers and degradation of adhesive monomers.
  • TENSILE PROPERTIES OF RESIN-INFILTRATED DEMINERALIZED HUMAN DENTIN
    H SANO, T TAKATSU, B CIUCCHI, CM RUSSELL, DH PASHLEY JOURNAL OF DENTAL RESEARCH 74 (4) 1093 -1102 1995年04月 [査読無し][通常論文]
     
    The ability of adhesive resins to restore the physical properties of demineralized dentin has not been well-documented. The unfilled resins that are used for adhesion have relatively low moduli of elasticity and limited ability to increase dentin stiffness, although they may increase the ultimate tensile strength of dentin. This study tested the hypothesis that resin infiltration of demineralized dentin can restore its tensile properties to those of mineralized dentin. Small (ca. 0.5 mm thick x 0.5 mm wide) specimens of demineralized human dentin were infiltrated with one of five different dentin bonding resins over many hours, to determine how these resins altered the tensile properties of dentin. Tensile stress and strain were measured in these and control (mineralized and demineralized) specimens until their ultimate failure. The results indicate that some adhesive resins, after infiltrating demineralized dentin, can restore and even exceed the ultimate tensile strength of mineralized dentin. These resins increased the modulus of elasticity of resin-infiltrated dentin to values equal to or greater than those of the resins but far below those of mineralized dentin. Although the conditions in this experiment were far removed from the manufacturer's recommendations or clinical practice, the results support the potential of resin infiltration for reinforcing dentin.
  • SEM APPROACH TO EVALUATE THE EFFECTS OF PRIMERS AND CONDITIONERS ON BONDED DENTIN SURFACE
    RM CARVALHO, M YOSHIYAMA, H SANO, B CIUCCHI, DH PASHLEY JOURNAL OF DENTAL RESEARCH 74 (3) 799 -799 1995年03月 [査読無し][通常論文]
  • NANOLEAKAGE - LEAKAGE WITHIN THE HYBRID LAYER
    H SANO, T TAKATSU, B CIUCCHI, JA HORNER, WG MATTHEWS, DH PASHLEY OPERATIVE DENTISTRY 20 (1) 18 -25 1995年01月 [査読無し][通常論文]
     
    Most microleakage studies involve quantitating the magnitude of movement of a tracer molecule through a gap between restorative materials and the wall of cavity preparations. The present microscopic study examined the migration of silver nitrate into the interface between dentin and five different dentin bonding agents used to restore class 5 cavities, in the absence of gap formation. Several different leakage patterns were seen, but they all indicated leakage within the hybrid layer when viewed by SEM. The ranking of microleakage from most to least was: All-Bond 2 > Superbond C&B > Scotchbond Multi-Purpose > Clearfil Liner Bond System > Kuraray Experimental System, KB-200. To distinguish this special type of microleakage within the basal, porous region of the hybrid layer in the absence of gap formation, we propose the term nanoleakage.
  • THEORETICAL BOND STRENGTH OF RESIN TO DENTIN
    DH PASHLEY, B CIUCCHI, H SANO, RM CARVALHO, CM RUSSELL JOURNAL OF DENTAL RESEARCH 74 150 -150 1995年 [査読無し][通常論文]
  • BONDING TO CARIES AFFECTED DENTIN
    M NAKAJIMA, H SANO, MF BURROW, T TAKATSU, M YOSHIYAMA, B CIUCCHI, DH PASHLEY JOURNAL OF DENTAL RESEARCH 74 36 -36 1995年 [査読無し][通常論文]
  • REGIONAL BOND STRENGTHS OF SELF-ETCHING SELF-PRIMING ADHESIVE SYSTEMS
    M YOSHIYAMA, S EBISU, R CARVALHO, H SANO, J HORNER, DH PASHLEY JOURNAL OF DENTAL RESEARCH 74 413 -413 1995年 [査読無し][通常論文]
  • TENSILE PROPERTIES OF RESIN-INFILTRATED DEMINERALIZED HUMAN DENTIN
    H SANO, T TAKATSU, B CIUCCHI, CM RUSSELL, DH PASHLEY JOURNAL OF DENTAL RESEARCH 74 33 -33 1995年 [査読無し][通常論文]
  • ADHESIVE MECHANISMS OF RESINS TO HUMAN ROOT DENTIN
    M YOSHIYAMA, RM CARVALHO, H SANO, J HORNER, DH PASHLEY JOURNAL OF DENTAL RESEARCH 74 33 -33 1995年 [査読無し][通常論文]
  • SEMおよびTEMを用いた接着界面の微小漏洩に関する研究
    佐野 英彦, 吉山 昌宏, 恵比須 繁之 日本歯科保存学雑誌 37 (6) 1686 -1693 1994年12月 [査読無し][通常論文]
     
    1)歯頸部5級窩洞における辺縁漏洩はAll Bond 2 (AB 2)のほうがClearfil Liner Bond System (CLBS)よりも有意に大きかった。2) 2種の接種システムにおける接着界面のSEM観察では,CLBSではhybrid層の下層部に銀の漏洩が認められた。AL 2では,hybrid層の管間象牙質部全層にわたって銀の侵入が認められた。3) TEM観察においては,CLBSではhybrid層の下層部に顆粒状に散在した銀の析出が認められた。AL 2ではhybrid層の全層とレジンタグの周囲に比較的密な網状の銀の侵入が認められた。4)今回の観察から両システムともnano leakageを引き起こすことが示唆された
  • H SANO, T SHONO, H SONODA, T TAKATSU, B CIUCCHI, R CARVALHO, DH PASHLEY DENTAL MATERIALS 10 (4) 236 -240 1994年07月 [査読無し][通常論文]
     
    Objectives. The purpose of this study was to test the null hypothesis that there is no relationship between the bonded surface area of dentin and the tensile strength of adhesive materials. Methods. The enamel was removed from the occlusal surface of extracted human third molars, and the entire flat surface was covered with resin composite bonded to the dentin to form a flat resin composite crown. Twenty-four hours later, the bonded specimens were sectioned parallel to the long axis of the tooth into 10-20 thin sections whose upper part was composed of resin composite with the lower half being dentin. These small sections were trimmed using a high speed diamond bur into an hourglass shape with the narrowest portion at the bonded interface. Surface area was varied by altering the specimen thickness and width. Tensile bond strength was measured using custom-made grips in a universal testing machine. Results. Tensile bond strength was inversely related to bonded surface area. At surface areas below 0.4 mm(2), the tensile bond strengths were about 55 MPa for Clearfil Liner Bond 2 (Kuraray Co., Ltd.), 38 MPa for Scotchbond MP (3M Dental Products), and 20 MPa for Vitremer (3M Dental Products). At these small surface areas all of the bond failures were adhesive in nature. Significance. This new method permits measurement of high bond strengths without cohesive failure of dentin. It also permits multiple measurements to be made within a single tooth.
  • TENSILE PROPERTIES OF MINERALIZED AND DEMINERALIZED HUMAN AND BOVINE DENTIN
    H SANO, B CIUCCHI, WG MATTHEWS, DH PASHLEY JOURNAL OF DENTAL RESEARCH 73 (6) 1205 -1211 1994年06月 [査読無し][通常論文]
     
    The relative contribution of the matrix of dentin to the physical properties of dentin is unknown but thought to be small. The objective of this study was to test the hypothesis that the demineralized matrix of dentin contributes little to the strength of dentin by measuring and comparing the ultimate tensile strength and modulus of elasticity of mineralized and demineralized dentin. Small slabs (4 x 0.5 x 0.5 mm) of bovine and human dentin were tested in a microtensile testing device in vitro. Human coronal mineralized dentin gave a mean ultimate tensile strength (UTS) of 104 MPa. Bovine incisor coronal dentin exhibited a UTS of 91 MPa, and bovine root dentin failed at 129 MPa. The modulus of elasticity of mineralized bovine and human dentin varied from 13 to 15 MPa. When dentin specimens were demineralized in EDTA, the UTS and modulus of elasticity fell to 26-32 MPa and 0.25 GPa, respectively, depending on dentin species. The results indicate that collagen contributes about 30% of the UTS of mineralized dentin, which is higher than was expected.
  • MICROPOROUS DENTIN ZONE BENEATH RESIN-IMPREGNATED LAYER
    H SANO, T SHONO, T TAKATSU, H HOSODA OPERATIVE DENTISTRY 19 (2) 59 -64 1994年03月 [査読無し][通常論文]
     
    The purpose of this study was to investigate marginal microleakage by examining the resin/dentin interface using a cryo-SEM and a silver ion penetration method. Class 5 cervical cavities were restored using an adhesive resin system following treatment with 37% phosphoric acid gel. The cavity wall adaptation was observed using cryo-SEM to avoid specimen damage. This observation showed excellent marginal adaptation of material to cavity walls and no gap formation. However, silver ions had penetrated beneath the resin-impregnated layer when observed with conventional SEM images, which showed the leakage of silver ions between the resin and decalcified tooth structure without gap formation. This study suggests that the bonding monomers of Clearfil Photo Bond were not fully able to penetrate into the demineralized dentin after phosphoric acid etching, thus leaving a porous zone as a microleakage pathway beneath the resin-impregnated layer.
  • Dentin as a Bording Substrate
    Deutsch Zahnerhalisch Zeitschift 49 (10) 760 -763 1994年 [査読無し][通常論文]
  • THE EFFECT OF SURFACE-AREA ON TENSILE BOND STRENGTH
    H SANO, EL PASHLEY, Y ZHANG, R CARVALHO, DH PASHLEY JOURNAL OF DENTAL RESEARCH 73 132 -132 1994年 [査読無し][通常論文]
  • TENSILE PROPERTIES OF HUMAN AND BOVINE DENTIN BEFORE AFTER DEMINERALIZATION
    H SANO, B CIUCCHI, WG MATTHEWS, DH PASHLEY JOURNAL OF DENTAL RESEARCH 73 277 -277 1994年 [査読無し][通常論文]
  • INFLUENCE OF DENTIN THICKNESS AND REGION ON TENSILE BOND STRENGTH
    S BOUILLAGUET, B CIUCCHI, H SANO, DH PASHLEY JOURNAL OF DENTAL RESEARCH 73 296 -296 1994年 [査読無し][通常論文]
  • NANOLEAKAGE - LEAKAGE WITHIN THE HYBRID LAYER
    H SANO, T TAKATSU, B CIUCCHI, JA HORNER, DH PASHLEY JOURNAL OF DENTAL RESEARCH 73 276 -276 1994年 [査読無し][通常論文]
  • BONDING TO SODIUM-HYPOCHLORITE TREATED DENTIN
    B CIUCCHI, H SANO, DH PASHLEY JOURNAL OF DENTAL RESEARCH 73 296 -296 1994年 [査読無し][通常論文]
  • Study on Marginal Leakage of the Adhesive Composite Restoration Adaptation vs Leakage
    Japanese Journal of Conservative Dentistry 36 (6) 1582 -1589 1993年 [査読無し][通常論文]
  • Permeability of dentin to adhesive agents
    Quintessence International 24 (9) 618 -631 1993年 [査読無し][通常論文]
  • DENTIN THICKNESS, HARDNESS, AND CA-CONCENTRATION VS BOND STRENGTH OF DENTIN ADHESIVES
    L PERINKA, H SANO, H HOSODA DENTAL MATERIALS 8 (4) 229 -233 1992年07月 [査読無し][通常論文]
     
    The relationship between the tensile bond strength (TBS) and three dentin characteristics: remaining dentin thickness (RDT), Ca-concentration, and hardness, were investigated. Sixty-two extracted bovine incisors, divided into four groups, were prepared using 600-grit SiC paper to create flat dentinal surfaces. The materials tested in this study consisted of three commercially available and one experimental dentin bonding systems. The TBS and modes of failure were determined after storage for 24 h in 37-degrees-C water. Then RDT, Ca-concentration, and hardness were measured. Data were statistically compared with TBS of each group. The mean bond strength of the commercial materials ranged from 2.5+/-0.9 MPato 7.7+/-2.8 MPa. Correlations with Ca-concentration and RDT varied, depending on the product. The bond strength of the experimental material was 9.2+/-4.4 MPa and significantly correlated with RDT and hardness. With two of the dentin bonding agents, specimens showed a high number of cohesive fractures in dentin, 40% to 82%. It was concluded that the variability of TBS was influenced by dentinal characteristics in three of the four materials tested.
  • Educational Evaluation and Analysis of Various Assessment Factors in operative Dentistry
    The Journal of Japanese Association for Dental Education 6 (2) 131 -139 1991年 [査読無し][通常論文]
  • Effect of high-speed Cutting on dentin permiability and bonding
    Dental Materials 7 (5) 1991年 [査読無し][通常論文]
  • RELATIONSHIP BETWEEN CARIES DETECTOR STAINING AND STRUCTURE OF CARIES DENTIN
    H SANO, H HOSODA JOURNAL OF DENTAL RESEARCH 67 (4) 729 -729 1988年04月 [査読無し][通常論文]
  • Classification and Elemental Composition of Fillers of Composite Resin Part1 Classification of Chemically Cured Composite Resins.
    30 (2) 1987年 [査読無し][通常論文]
  • Clinical Evaluation of Posterior Restorative Composite Resins-Three to Four Year Assessment
    30 (4) 1987年 [査読無し][通常論文]
  • Relationship Between Caries Detector Staining and Structural Chatacteristics of Caries Dentin
    54 (1) 1987年 [査読無し][通常論文]
  • Clinical Evaluation of Posterior Restorative Composites-Two Year Assessment-
    28 (3) 1985年 [査読無し][通常論文]

受賞

  • 2019年02月 Academy of Operative Dentistry Hollenback Memorial Prize
     Enormous contribution to research and education of Operative Dentistry 
    受賞者: HIdehiko Sano
  • 2003年 デンツサプライ賞
  • 2003年 Dentspy award

共同研究・競争的資金等の研究課題

  • ナノテクノロジーの歯科への展開
    研究期間 : 2007年
  • 修復物のbiodegradationの研究
  • 修復物の辺縁漏洩に関する研究
  • 象牙質の物理的特性に関する研究
  • 接着界面の形態に関する研究
  • Biodegradation of restorations
  • Marginal leakage of restorations
  • Mechanical propaties of dentin
  • Morphological approach to the adhesive interface

教育活動情報

主要な担当授業

  • 大学院共通授業科目(一般科目):自然科学・応用科学
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 大学院共通科目
  • 口腔生物学と医学
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
  • 口腔生物学と医学
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
  • 歯内療法学
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 歯内療法
  • 歯内療法学
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 歯内療法
  • 歯内療法学研究Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 歯内療法
  • 歯内療法学研究Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 歯内療法
  • 歯内療法学研究Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 歯内療法
  • 歯内療法学研究Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 歯内療法
  • 発表・論文執筆法演習Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 研究計画,文献検索,研究のまとめ,口頭発表,論文作製
  • 発表・論文執筆法演習Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 研究計画,文献検索,研究のまとめ,口頭発表,論文作製
  • 発表・論文執筆法演習Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 研究計画,文献検索,研究のまとめ,口頭発表,論文作製
  • 発表・論文執筆法演習Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 研究計画,文献検索,研究のまとめ,口頭発表,論文作製
  • 発表・論文執筆法演習Ⅲ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 研究計画,文献検索,研究のまとめ,口頭発表,論文作製
  • 発表・論文執筆法演習Ⅲ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 研究計画,文献検索,研究のまとめ,口頭発表,論文作製
  • 口腔健康科学実習Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅲ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅲ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅳ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅳ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅴ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 口腔健康科学実習Ⅴ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : Operative Dentistry, Cariology, Biocompatibility, Adhesive Dentistry, Esthetic Dentistry, MI
  • 歯冠修復学研究Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 接着歯学
  • 歯冠修復学研究Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 接着歯学
  • 歯冠修復学研究Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 歯冠修復
  • 歯冠修復学研究Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 歯冠修復
  • 接着歯学研究Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 接着歯学
  • 接着歯学研究Ⅰ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 接着歯学
  • 接着歯学研究Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 接着歯学
  • 接着歯学研究Ⅱ
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 接着歯学
  • 齲触制御・保存修復学
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学研究科
    キーワード : 歯科保存学
  • 齲触制御・保存修復学
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 歯学院
    キーワード : 歯科保存学
  • 保存修復学臨床実習Ⅰ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 歯学部
    キーワード : 保存修復学、臨床実習
  • 保存修復学臨床実習Ⅱ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 歯学部
    キーワード : 保存修復学、臨床実習
  • 統合講義
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 歯学部
    キーワード : 学生参加型、問題解決能力、全体発表
  • 臨床講義Ⅲ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 歯学部
    キーワード : 臨床、患者、コデンタルスタッフ、保険
  • 保存修復学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 歯学部
    キーワード : 保存修復学
  • 保存修復学Ⅰ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 歯学部
    キーワード : 保存修復学
  • 保存修復学基礎実習Ⅰ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 歯学部
    キーワード : 保存修復学、模型実習
  • 保存修復学基礎実習Ⅱ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 歯学部
    キーワード : 保存修復学、模型実習

大学運営

委員歴

  • 2001年   国際歯科学会(International Association for Dental Research)   Editorial Board   国際歯科学会(International Association for Dental Research)
  • 1997年   日本歯科保存学会   理事   日本歯科保存学会
  • 1997年   日本歯科理工学会   評議員   日本歯科理工学会
  • 1997年   接着歯学   理事   接着歯学
  • 1997年   口腔病学会   理事   口腔病学会


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