研究者データベース

近藤 祥子(コンドウ ヨシコ)
保健科学研究院 保健科学部門 創成看護学分野
准教授

基本情報

所属

  • 保健科学研究院 保健科学部門 創成看護学分野

職名

  • 准教授

学位

  • 博士(バイオサイエンス)(奈良先端科学技術大学院大学)

科研費研究者番号

  • 40423248

J-Global ID

研究キーワード

  • 発生生物学   分子生物学   メンタルヘルス   助産   看護   周産期   ウィメンズヘルス   

研究分野

  • ライフサイエンス / 生涯発達看護学 / 母性看護・助産学

担当教育組織

職歴

  • 2022年03月 - 現在 北海道大学大学院保健科学研究院 創成看護学分野 准教授
  • 2020年04月 - 2022年02月 京都大学 大学院医学研究科人間健康科学系専攻 講師
  • 2018年04月 - 2020年03月 京都大学 医学部人間健康科学系専攻 助教
  • 2016年04月 - 2018年03月 京都第一赤十字病院 総合周産期母子医療センター 助産師
  • 2015年04月 - 2016年03月 神戸市立西神戸医療センター 周産期センター 助産師
  • 2010年04月 - 2011年03月 理化学研究所 発生・再生科学総合研究センター 博士研究員
  • 2007年04月 - 2010年03月 自然科学研究機構 岡崎統合バイオサイエンスセンター 博士研究員
  • 2005年04月 - 2007年03月 奈良先端科学技術大学院大学 博士研究員

学歴

  • 2011年04月 - 2014年03月   神戸看護専門学校
  • 2000年04月 - 2005年03月   奈良先端科学技術大学院大学   バイオサイエンス研究科
  • 1996年04月 - 2000年03月   京都大学   農学部   生物機能科学科
  •         -   神戸市看護大学   助産学専攻

研究活動情報

論文

  • 帝王切開を経験した女性の出産に対する心情に関する質的研究
    乾沙帆, 新福洋子, 松本早織, 近藤祥子, 古田真里枝
    京都母性衛生学会誌 27 1 37 - 44 2019年05月 [査読有り][通常論文]
  • 搬送先医療機関で助産所助産師の介助により分娩した事例
    近藤 祥子, 藤井ひろみ
    兵庫県母性衛生学会雑誌 24 21 - 24 2015年 [査読無し][通常論文]
  • Helene Chanut-Delalande, Yoshiko Hashimoto, Anne Pelissier-Monier, Rebecca Spokony, Azza Dib, Takefumi Kondo, Jerome Bohere, Kaori Niimi, Yvan Latapie, Sachi Inagaki, Laurence Dubois, Philippe Valenti, Cedric Polesello, Satoru Kobayashi, Bernard Moussian, Kevin P. White, Serge Plaza, Yuji Kageyama, Francois Payre
    NATURE CELL BIOLOGY 16 11 1035 - + 2014年11月 [査読有り][通常論文]
     
    Animal development fundamentally relies on the precise control, in space and time, of genome expression. Whereas we have a wealth of information about spatial patterning, the mechanisms underlying temporal control remain poorly understood. Here we show that Pri peptides, encoded by small open reading frames, are direct mediators of the steroid hormone ecdysone for the timing of developmental programs in Drosophila. We identify a previously uncharacterized enzyme of ecdysone biosynthesis, GstE14, and find that ecdysone triggers pri expression to define the onset of epidermal trichome development, through post-translational control of the Shavenbaby transcription factor. We show that manipulating pri expression is sufficient to either put on hold or induce premature differentiation of trichomes. Furthermore, we find that ecdysone-dependent regulation of pri is not restricted to epidermis and occurs over various tissues and times. Together, these findings provide a molecular framework to explain how systemic hormonal control coordinates specific programs of differentiation with developmental timing.
  • Yuji Kageyama, Takefumi Kondo, Yoshiko Hashimoto
    BIOCHIMIE 93 11 1981 - 1986 2011年11月 [査読有り][通常論文]
     
    Genome analysis has identified a number of putative non-protein-coding transcripts that do not contain ORFs longer than 100 codons. Although evidence strongly suggests that non-coding RNAs are important in a variety of biological phenomena, the discovery of small peptide-coding mRNAs confirms that some transcripts that have been assumed to be non-coding actually have coding potential. Their abundance and importance in biological phenomena makes the sorting of non-coding RNAs from small peptide-coding mRNAs a key issue in functional genomics. However, validating the coding potential of small peptide-coding RNAs is complicated, because their ORF sequences are usually too short for computational analysis. In this review, we discuss computational and experimental methods for validating the translatability of these non-coding RNAs. (C) 2011 Elsevier Masson SAS. All rights reserved.
  • T. Kondo, S. Plaza, J. Zanet, E. Benrabah, P. Valenti, Y. Hashimoto, S. Kobayashi, F. Payre, Y. Kageyama
    SCIENCE 329 5989 336 - 339 2010年07月 [査読有り][通常論文]
     
    A substantial proportion of eukaryotic transcripts are considered to be noncoding RNAs because they contain only short open reading frames (sORFs). Recent findings suggest, however, that some sORFs encode small bioactive peptides. Here, we show that peptides of 11 to 32 amino acids encoded by the polished rice (pri) sORF gene control epidermal differentiation in Drosophila by modifying the transcription factor Shavenbaby (Svb). Pri peptides trigger the amino-terminal truncation of the Svb protein, which converts Svb from a repressor to an activator. Our results demonstrate that during Drosophila embryogenesis, Pri sORF peptides provide a strict temporal control to the transcriptional program of epidermal morphogenesis.
  • Yoshiko Hashimoto, Takefumi Kondo, Yuji Kageyama
    DEVELOPMENT GROWTH & DIFFERENTIATION 50 Suppl 1 S269 - S276 2008年06月 [査読有り][通常論文]
     
    Generally, bioactive small peptides are derived from precursors with signal sequences at their N-terminal ends, which undergo modification and proteolysis through a secretory pathway. By contrast, small peptides encoded in short open reading frames (sORF) lack signaling sequences and therefore are released into the cytoplasm, which may result in their having functions distinct from those of secreted peptides. Several small peptides encoded by sORF are involved in the morphogenesis of multicellular organisms. POLARIS, ROTUNDIFOLIA4, and Enod40 are plant peptides that are involved, respectively, in root formation, leaf shape control, and cortical cell division during nodule formation. Brick1/HSPC300 is an evolutionarily conserved component of the actin reorganization complex. polished rice/tarsal-less and mille-pattes encode related small peptides that are required for epithelial morphogenesis in Drosophila and segmentation in Tribolium. There are only a few known examples of small peptides encoded by sORF, and their molecular functions are still largely obscure. Nevertheless, an increasing number of sORF genes is being identified, and further research should reveal their roles in novel molecular mechanisms underlying developmental events.
  • Takefumi Kondo, Yoshiko Hashimoto, Kagayaki Kato, Sachi Inagaki, Shigeo Hayashi, Yuji Kageyama
    NATURE CELL BIOLOGY 9 6 660 - U87 2007年06月 [査読有り][通常論文]
     
    Transcriptome analyses in eukaryotes, including mice and humans, have identified polyA- containing transcripts that lack long open reading frames (ORFs; > 100 amino acids) 1,2. These transcripts are believed most likely to function as non-coding RNAs, but their translational capacities and biological activities have not been characterized in detail. Here, we report that polished rice (pri), which was previously identified as a gene for a non-coding RNA in Drosophila3,4, is in fact transcribed into a polycistronic mRNA that contains evolutionarily conserved short ORFs that encode 11 or 32 amino acid-long peptides. pri was expressed in all epithelial tissues during embryogenesis. The loss of pri function completely eliminated apical cuticular structures, including the epidermal denticles and tracheal taenidia, and also caused defective tracheal-tube expansion. We found that pri is essential for the formation of specific F-actin bundles that prefigures the formation of the denticles and taenidium. We provide evidences that pri acts non-cell autonomously and that four of the conserved pri ORFs are functionally redundant. These results demonstrate that pri has essential roles in epithelial morphogenesis by regulating F-actin organization.
  • Y Hashimoto, H Suzuki, Y Kageyama, K Yasuda, K Inoue
    GENE EXPRESSION PATTERNS 6 2 201 - 205 2006年01月 [査読有り][通常論文]
     
    Maternally supplied germ plasm is essential for germ lineage establishment in many species, but the molecular details are still largely unknown, especially in vertebrates, and identification of novel factors that localize to germ plasm is desirable. We previously reported that one of the components of zebrafish germ plasm is mRNA of the bruno-like (brul) gene, a homologue of bruno, which, in Drosophila, is known to participate in germ lineage establishment. Here, we show that not only mRNA but also protein of brul is localized to the zebrafish germ plasm at the ends of the cleavage furrows. In 4- and 8-cell stage embryos, Brul protein is localized to the periphery of the blastomeres, as well as to the ends of the cleavage furrows, forming numerous minute particles. These particles appear at the cortex of the fertilized egg within 10 min after fertilization. Surprisingly, these distinctive localizations, as well as the minute particles, completely disappeared by the 16-cell stage, although relatively weak expression was detected ubiquitously throughout embryogenesis. This is the first report of a protein that localizes to the germ plasm in zebrafish. (C) 2005 Elsevier B.V. All rights reserved.
  • Y Hashimoto, S Maegawa, T Nagai, E Yamaha, H Suzuki, K Yasuda, K Inoue
    DEVELOPMENTAL BIOLOGY 268 1 152 - 161 2004年04月 [査読有り][通常論文]
     
    Maternally supplied factors in fertilized eggs play essential roles in the establishment of primordial germ cells. In zebrafish, cytoplasm at the distal ends of the first and second cleavage furrows has been assumed to contain germ lineage determinants, since maternal transcripts of genri lineage-specific genes are localized to ends of the cleavage furrows. To investigate whether these parts of cytoplasm are required for germ cell formation, we removed all four regions of the cytoplasm by glass capillary at the 4-cell stage. Histological analysis revealed that the ablation of cytoplasm at the ends of the cleavage planes resulted in a severe reduction in the number of germ cells. In addition, the expression of germ lineage markers was eliminated by cytoplasmic ablation. These results demonstrated that cytoplasm at the distal ends of cleavage furrows is essential for germ cell formation. We also found novel localization patterns for zDazl and brul mRNAs along the cleavage planes. Our findings provide the first direct evidence that localized cytoplasmic factors are indispensable for germ cell establishment in zebrafish. (C) 2004 Elsevier Inc. All rights reserved.

講演・口頭発表等

  • 大学院助産課程における分娩介助シミュレーション実践報告  [通常講演]
    近藤祥子
    日本看護シミュレーションラーニング学会 2022年02月
  • 山澤ほのか, 古田真里枝, 近藤祥子
    第29回 京都母性衛生学会学術集会 2021年07月 口頭発表(一般)
  • A needs assessment in antenatal education on caesarean birth in Japan – a cross-sectional study  [通常講演]
    Yoshiko H Kondo, Satoshi Sohda, Shigehito Yamada, Marie Furuta
    32nd ICM Virtual Triennial Congress 2021年06月 口頭発表(一般)
  • 帝王切開産前教育の産後うつ症状に対する効果検証 –ランダム化比較試験-  [通常講演]
    竹安永花里, 古田真里枝, 近藤洋子
    第21回香川母性衛生学会学術集会 2020年12月 口頭発表(一般)
  • ランダム化比較試験による産後精神障害予防を目的とした帝王切開産前教育モデルの効果―研究プロトコルー  [通常講演]
    増井莉菜, 近藤祥子, 古田真里枝
    第28回京都母性衛生学会学術集会 2019年07月
  • 1型糖尿病合併妊娠における分娩時血糖コントロールの比較:症例検討  [通常講演]
    勝島倫子, 西村亜希子, 原島伸一, 千種義継, 近藤 祥子, 古田真里枝
    第27 回京都母性衛生学会学術集会 2018年07月
  • 搬送先医療機関で助産所助産師の介助により分娩した事例  [通常講演]
    近藤祥子, 藤井ひろみ
    2015年06月 口頭発表(一般)
  • A small peptide gene, polished rice, participates in Drosophila ecdysone signal pathway  [招待講演]
    Yoshiko Hashimoto, K. Niimi, T.Kondo, Y. Kageyama
    The 18th International Ecdysone Workshop 2010年07月
  • polished rice functions in ecdysone signaling.  [通常講演]
    Yoshiko Hashimoto, T. Kondo, Y. Kageyama
    50th Annual Drosophila Research Conference, 2009年04月
  • ショウジョウバエ変態期における細胞非自律的なpolished riceの機能  [通常講演]
    橋本祥子, 近藤武史, 影山裕二
    日本動物学会大会 2008年08月
  • A short peptide gene, polished rice, controls Drosophila metamorphosis through intercellular communication.  [通常講演]
    Y. Hashimoto, T. Kondo, Y. Kageyama近藤
    第41回日本発生生物学会 2008年05月 口頭発表(一般)
  • A short peptide gene, polished rice, regulate Drosophila imaginal development via cell-cell interaction.  [通常講演]
    Y. Hashimoto, T. Kondo, Y. Kageyama
    第8回日本ショウジョウバエ研究集会 2007年07月
  • ショウジョウバエ短鎖ペプチド遺伝子polished riceは細胞自律的に機能する  [通常講演]
    橋本祥子, 近藤武史, 影山裕二
    第40回 日本発生生物学会年会 2007年05月
  • The role of a short peptide gene polished rice during imaginal development.  [通常講演]
    Yoshiko Hashimoto, T. Kondo, S.Inagaki, Y. Kageyama
    48th Annual Drosophila Research Conference 2007年03月
  • Localized maternal factors are required for zebrafish germ cell formation  [通常講演]
    Y. Hashimoto, S. Maegawa, K. Yasuda, K. Inoue
    15th Congress of the International Society of Developmental Biology 2005年09月
  • Requirement of localized Maternal Factors for Zebrafish Germ Cell Formation  [通常講演]
    Y. Hashimoto, S. Maegawa, K. Yasuda, K. Inoue
    62nd Annual Meeting Jointly with the International Society of Developmental Biology 2003年07月
  • Requirement of localized maternal factors for zebrafish germ cell formation  [通常講演]
    Y. Hashimoto, S. Maegawa, K. Yasuda, K. Inoue
    5th International Meeting of zebrafish Development and Genetics 2002年06月

その他活動・業績

受賞

  • 2005年03月 奈良先端科学技術大学院大学 最優秀学生賞
     ゼブラフィッシュにおける生殖細胞の運命決定機構 - 卵割面両端に局在する母性供与因子の生理機能 - 
    受賞者: 橋本 祥子
  • 2004年 奈良先端科学技術大学院大学 第1回梅園賞
     ゼブラフィッシュ生殖細胞形成に置ける母性供与因子群の役割 
    受賞者: 橋本 祥子

共同研究・競争的資金等の研究課題

  • 産後PTSDは「筆記表現法」で予防できるか?〜指針策定に向けて
    文部科学省:科学研究費補助金 基盤研究(B)
    研究期間 : 2019年04月 -2022年03月 
    代表者 : 古田 真里枝
  • 生殖補助医療を受けた妊産婦の周産期メンタルヘルスケアー不安を中心とした量的解析
    文部科学省:科学研究費補助金 基盤研究(C)
    研究期間 : 2019年04月 -2022年03月 
    代表者 : 近藤 祥子
  • 京都大学:平成30年度第Ⅱ期 京都大学若手研究者スタートアップ研究費
    研究期間 : 2018年10月 -2019年03月 
    代表者 : 近藤 祥子

大学運営

委員歴

  • 2022年04月 - 現在   日本助産学会   広報委員
  • 2022年06月 - 2023年01月   全国助産師教育協議会   助産師国家試験プール問題作成・登録協力員
  • 2020年04月 - 2022年02月   京都母性衛生学会   理事
  • 2018年04月 - 2020年03月   京都母性衛生学会   幹事


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