研究者データベース

金 尚昊(キン サンホ)
獣医学研究院 獣医学部門 臨床獣医科学分野
助教

基本情報

所属

  • 獣医学研究院 獣医学部門 臨床獣医科学分野

職名

  • 助教

学位

  • 学士

J-Global ID

研究活動情報

論文

  • Kim S, Hosoya K, Takagi S, Okumura M
    Journal of the American Veterinary Medical Association 255 3 330 - 335 2019年08月 [査読有り][通常論文]
  • Deguchi T, Hosoya K, Murase Y, Koangyong S, Kim S, Okumura M
    Veterinary and comparative oncology 17 2 119 - 129 2019年06月 [査読有り][通常論文]
  • Suranji Wijekoon HM, Kim S, Bwalya EC, Fang J, Aoshima K, Hosoya K, Okumura M
    Research in veterinary science 122 179 - 185 2019年02月 [査読有り][通常論文]
  • Takagi S, Kagawa Y, Hanazono K, Murakami S, Deguchi T, Izumi Y, Hosoya K, Kim S, Okumura M
    The Journal of veterinary medical science 80 9 1456 - 1458 2018年09月 [査読有り][通常論文]
  • Bwalya EC, Wijekoon HS, Fang J, Kim S, Hosoya K, Okumura M
    The Journal of veterinary medical science 2018年09月 [査読有り][通常論文]
  • Kim S, Hosoya K, Kobayashi A, Okumura M
    Veterinary and comparative oncology 2018年09月 [査読有り][通常論文]
  • H.M.Suranji Wijekoon, Eugene C. Bwalya, Jing Fang, Sangho Kim, Kenji Hosoya, Masahiro Okumura
    BMC Veterinary Research 14 1 152  2018年05月02日 [査読有り][通常論文]
     
    Background: Sodium pentosan polysulfate (NaPPS) was testified as a chondroprotective drug in with a detailed rationale of the disease-modifying activity. This study was undertaken to determine whether anti-osteoarthritis drug, NaPPS inhibited osteoclasts (OC) differentiation and function. Canine bone marrow mononuclear cells (n = 6) were differentiated to OC by maintaining with receptor activator of nuclear factor kappa B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) for up to 7 days with the treatment of NaPPS at concentration of 0, 0.2, 1 and 5 μg/mL. Differentiation and function of OC were accessed using tartrate-resistant acid phosphate (TRAP) staining and bone resorption assay, while monitoring actin ring formation. Invasion and colocalization patterns of fluorescence-labeled NaPPS with transcribed gene in OC were monitored. Gene expression of OC for cathepsin K (CTK), matrix metallopeptidase-9 (MMP-9), nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), c-Fos, activator protein-1(AP-1) and carbonic anhydrase II was examined using real-time PCR. Results: Significant inhibition of OC differentiation was evident at NaPPS concentration of 1 and 5 μg/mL (p < 0.05). In the presence of 0.2 to 5 μg/mL NaPPS, bone resorption was attenuated (p < 0.05), while 1 and 5 μg/mL NaPPS achieved significant reduction of actin ring formation. Intriguingly, fluorescence-labeled NaPPS invaded in to cytoplasm and nucleus while colocalizing with actively transcribed gene. Gene expression of CTK, MMP-9 and NFATc1 were significantly inhibited at 1 and 5 μg/mL (p < 0.05) of NaPPS whereas inhibition of c-Fos and AP-1 was identified only at concentration of 5 μg/mL (p < 0.05). Conclusions: Taken together, all the results suggest that NaPPS is a novel inhibitor of RANKL and M-CSF-induced CTK, MMP-9, NFATc1, c-Fos, AP-1 upregulation, OC differentiation and bone resorption which might be a beneficial for treatment of inflammatory joint diseases and other bone diseases associated with excessive bone resorption.
  • H. M. Suranji Wijekoon, Kazuhide Toyota, Sangho Kim, Jing Fang, Eugene C. Bwalya, Kenji Hosoya, Masahiro Okumura
    RESEARCH IN VETERINARY SCIENCE 114 370 - 377 2017年10月 [査読有り][通常論文]
     
    The objective of this study was to assess the differentiation capability of synoviocytes derived from dogs with inflammatory joint conditions. Cranial cruciate ligament ruptured (CCLr) (n = 12) and medial patella luxated (MPL) (n = 10) knee joints of the dogs were used to collect the synovial membrane (SM). Synoviocytes were enzymatically released from the SM and analyzed by flow cytometry for specific cellular markers (CD44 and CD90) of mesenchymal stem cells (MSCs), while doing histopathology from another part of SM sections. Under specific culture conditions, synoviocytes were forced to differentiate into chondrogenesis, adipogenesis, osteogenesis and osteoclastogenesis to investigate the multipotency. Upon treatments phenotypes of cell cultures were analyzed by histopathology and by semi-quantitative reverse transcriptase polymerase chain reaction for the expression of each differentiation marker genes. Although flow cytometry showing similar MSCs populations in CCLr and MPL synovium, synovial cells derived from CCLr showed higher multipotency compared to MPL-derived samples. Further, synovial changes such as vascularity, mononuclear cell infiltration and cellular hypertrophy were more pronounced in CCLr-derived synovial tissue than in MPL. Taken together, these findings suggested that the differentiation capability of SM-derived multipotent stem cells varies with inflammatory severity occurring in different joint conditions.
  • Eugene C. Bwalya, Sangho Kim, Jing Fang, H. M. Suranji Wijekoon, Kenji Hosoya, Masahiro Okumura
    JOURNAL OF VETERINARY MEDICAL SCIENCE 79 7 1182 - 1190 2017年07月 [査読有り][通常論文]
     
    Mesenchymal stem cells (MSC) are a potential alternative source of differentiated chondrocytes for cartilage tissue regeneration and repair of osteoarthritic (OA) joints. We investigated the effects of pentosan polysulfate (PPS) and polysulfated glycosaminoglycan (PSGAG) on chondrogenesis of canine bone marrow-derived mesenchymal stem cells (cBMSC) in alginate and micromass cultures (MMC). Chondrogenic differentiation medium (CDM) was supplemented with PPS or PSGAG at concentrations of 0 (positive control; PC), 1, 3 and 5 mu g/ml. 10% DMEM was used as negative control. Chondrocyte phenotype was analyzed by quantitative real-time PCR (qPCR) for alginate cultures and Alcian blue staining for proteoglycan (PG) synthesis for MMC. In alginate culture, PPS and PSGAG showed no significant effect on type II collagen, aggrecan and HIF-2 alpha mRNA expression. PPS had no significant effect on type I collagen whereas PSGAG significantly upregulated (P<0.05) it at all concentrations relative to other treatments. PPS demonstrated a dose-dependent inhibitory effect on type X collagen mRNA with significant inhibition observed at 5 mu g/ml compared to the NC. PSGAG showed an inverse effect on type X collagen with 1 mu g/ml significantly inhibiting its expression while increase in the concentration correspondingly increased type X collagen expression. In MMC, PPS significantly enhanced chondrogenesis and PG deposition whereas PSGAG inhibited chondrogenesis and promoted a fibrocartilage-like phenotype with reduced PG deposition. While PPS enhances chondrogenesis of cBMSC in MMC, the response of MSC to chondroinductive factors is culture system-dependent and varies significantly between alginate and MMC.
  • Bwalya EC, Kim S, Fang J, Wijekoon HMS, Hosoya K, Okumura M
    PloS one 12 5 e0177144  2017年05月 [査読有り][通常論文]
     
    Osteoarthritic (OA) chondrocytes are shown to express inducible nitric oxide synthase (iNOS) which produces high concentrations of nitric oxide (NO), particularly when stimulated with proinflammatory cytokines. NO is involved in OA cartilage degradation. On the other hand, c-Jun N-terminal Kinase (JNK) pathway mediates the activation and transcription of c-Jun, which is required for interleukin-1 (IL-1)-induction of matrix metalloproteinases-13 (MMP-13) in OA pathogenesis. Therefore, the selective inhibition of iNOS and c-Jun is a promising target for treatment and prevention of OA. The purpose of the study was to investigate the inhibitory effects of pentosan polysulfate (PPS) on IL-1 beta-induced iNOS, c-Jun and HIF-alpha isoforms upregulation in canine articular chondrocytes (CACs). Primary (P0) chondrocytes were isolated and cultured from femoral head cartilages of three (3) dogs. First passage (P1) chondrocytes were preincubated with 0, 1, 5, 15 and 40 mu g/mL of PPS for 4 hr before treatment with 10 ng/mL rhIL-1 beta for a further 8 hr. In addition, we evaluated the effects of single and multiple cytokine with or without LPS on iNOS protein induction. PPS significantly inhibited (P < 0.05) IL-1 beta-induced iNOS, c-Jun and HIF-1 alpha mRNA upregulation in a dose-dependent pattern. iNOS mRNA was significantly inhibited at 15 and 40 mu g/mL whereas c-Jun and HIF-1 alpha were significantly downregulated at 5, 15 and 40 mu g/mL of PPS compared to chondrocytes treated with only rhIL-1 beta. Intriguingly, CACs were recalcitrant to single IL-1 beta, TNF-alpha or LPS-induction of iNOS protein including to a combination of IL-1 beta+TNF-alpha, IL-1 beta+LPS except to TNF-alpha+LPS and IL-1 beta+TNF-alpha+LPS suggestive of a protective mechanism from iNOS detrimental effects on perpetuating OA. IL-1 beta+TNF-alpha+LPS-induced iNOS protein expression was significantly abrogated by PPS. We demonstrate for the first time that PPS is a novel inhibitor of IL-1 beta-induced iNOS, c-Jun, and HIF-1 alpha mRNA upregulation and iNOS protein induction which may be beneficial for prevention and treatment OA.
  • Suranji Wijekoon, Eugene C. Bwalya, Jing Fang, Sangho Kim, Kenji Hosoya, Masahiro Okumura
    Journal of Veterinary Medical Science 79 12 2030 - 2035 2017年 [査読有り][通常論文]
     
    The aim of this study was to investigate osteoclastogenic properties of inflammatory cytokines at different time-points of osteoclastogenesis. Bone marrow-derived macrophages from five healthy dogs were stimulated with the macrophage colony-stimulating factor, receptor activator of nuclear factor-κB ligand and inflammatory cytokines such as interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-17. Osteoclasts (OC) formation and function were enhanced with TNF-α regardless of temporal differences. But in contrast, IL-1β suppressed the osteoclastogenesis at early phase of the process while upregulating at the late phase. Furthermore, differentiation of OC precursors into OC was suppressed at high concentrations of IL-17. Collectively, the results revealed that suppressing TNF-α would be a promising strategy to inhibit inflammation-associated bone destruction in dogs.
  • Sangho Kim, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION 51 6 407 - 412 2015年11月 [査読有り][通常論文]
     
    An 8 yr old, intact male Shiba Inu was presented with loose stool, polydipsia, hematuria, vomiting, and anorexia. On abdominal ultrasonography, numerous nodules were detected in the hepatic parenchyma distributed diffusely throughout all lobes. Excisional biopsy of one of the nodules was performed via exploratory laparotomy. A histopathological diagnosis of the lesion was carcinoid, and the tumor cells stained positive to chromogranin A and gastrin. The serum gastrin level of the dog was 45,613 pg/mL (reference range: 160-284). In addition to medical treatment with omeprazole(c) and famotidine(e), suppression of gastrin secretion was attempted with octreotide acetate. A test dose of octreotide acetate significantly decreased the serum gastrin level to approximately one third of the baseline in 2 hr and the effect lasted approximately for 6 hr. On day 21, treatment with sustained-release formulation of octreotide acetate(a) (5 mg intramuscular, q 4 wk) was initiated. The serum gastrin concentration gradually decreased over 32 days and then progressively increased in parallel with the progression of the hepatic nodules. The dog gradually developed recurrence of initial clinical signs, and was lost to follow-up on day 510.

教育活動情報

主要な担当授業

  • 先端獣医科学特論A 最新獣医外科学特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 軟部組織外科学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 軟部組織、眼科、外科適応疾患、麻酔法、保存療法、手術療法、術後管理
  • 先端獣医科学特論A 国際獣医科学特論:臨床獣医学
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 外科学総論
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 獣医外科学、外科的侵襲、病態生理、損傷、創傷
  • 獣医科学基礎科目 臨床疾病学特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 臨床腫瘍学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 臨床獣医学、獣医腫瘍学
  • 先端獣医科学科目 最新獣医外科学特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 整形外科学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 獣医整形外科学、骨、関節、靭帯、腱、蹄
  • 総合専門臨床特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 伴侶動物夜間・救急獣医療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 夜間診療、救急処置
  • 伴侶動物獣医療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 伴侶動物獣医療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 伴侶動物、内科、外科、検査、診断、治療
  • 伴侶動物夜間・救急獣医療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 応用外科学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 応用 外科
  • アドバンスト演習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 伴侶動物外科学実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 外科手術、麻酔
  • 産業動物外科学実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 産業動物(牛、馬)、外科、麻酔


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