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Last Updated :2024/07/27

研究者情報

マスター

アカウント（マスター）

  • 氏名

    寳金　清博(ホウキン　キヨヒロ), ホウキン　キヨヒロ

所属（マスター）

  • 総長、理事・副学長

所属（マスター）

  • 総長、理事・副学長

独自項目

PositionHistory

  • 教育研究評議会評議員, 2013年4月1日, 2016年3月31日
  • 教育研究評議会評議員, 2016年4月1日, 2019年3月31日
  • 国際連携研究教育局長, 2020年10月1日
  • 副学長, 2017年4月1日, 2019年3月31日
  • 副理事, 2013年4月1日, 2016年3月31日
  • 副理事, 2016年4月1日, 2017年3月31日
  • 北海道大学病院長, 2013年4月1日, 2016年3月31日
  • 北海道大学病院長, 2016年4月1日, 2019年3月31日

researchmap

プロフィール情報

学位

  • 博士（北海道大学）

プロフィール情報

  • 姓

    寳金, ホウキン

  • 名

    清博, キヨヒロ

  • ID各種

    200901022425937792

通称等の別名

    宝金 清博

対象リソース

業績リスト

研究キーワード

  • 血管内皮前駆細胞   human   Linkage analysis   Moyamoya disease   サイトカイン   Microsatellite analysis   Mitochondria   遺伝子   脳血管   cytokine receptor   Brain   Cardiopulmonary arrest   Apoptosis   Malignant glioma   もやもや病   growth factor   positional cloning   Bcl-2   Receptor   Neurofibromatosis type 1   腫瘍免疫   cytokine   autocrine loop   gene expression   脳神経疾患   Chromosome 17   イオンチャネル   脳腫瘍   Neurosurgery   

研究分野

  • ライフサイエンス / 脳神経外科学

経歴

  • 2020年10月 - 現在 北海道大学 総長
  • 2019年09月 - 2020年09月 北海道大学 大学院保健科学研究院 高次脳機能創発分野 特任教授
  • 2017年04月 - 2019年03月 北海道大学 副学長
  • 2013年04月 - 2019年03月 北海道大学病院 病院長
  • 2010年03月 - 2019年03月 北海道大学 大学院医学研究科脳神経外科学分野 教授
  • 2013年04月 - 2017年03月 北海道大学 副理事
  • 2006年04月 - 2010年03月 札幌医科大学附属病院 副院長
  • 2001年11月 - 2010年03月 札幌医科大学脳神経外科 教授
  • 2000年11月 - 2001年10月 北海道大学 大学院医学研究科脳神経外科学分野 助教授
  • 1992年06月 - 2000年10月 北海道大学 大学院医学研究科脳神経外科学分野 講師
  • 1992年04月 - 1992年05月 北海道大学 大学院医学研究科脳神経外科学分野 助手

学歴

  • 1996年 - 1996年   米国スタンフォード大学・英国王立神経研究所   文部科学省在外研究員
  • 1986年 - 1989年   米国カリフォルニア大学デービス校   客員研究員
  • 1973年04月 - 1979年03月   北海道大学   医学部   医学科

委員歴

  • 2014年10月 - 現在   日本学術会議   会員
  • - 現在   国際観光医療学会   理事
  • - 現在   国際臨床医学会   理事
  • - 現在   日本脳卒中学会   理事
  • - 現在   日本脳循環代謝学会   理事
  • - 現在   日本脳神経外科学会   理事

受賞

  • 2018年10月 伊藤太郎学術賞
  • 2017年10月 斎藤眞賞
     
    受賞者: 寳金 清博
  • 2013年02月 公益信託美原脳血管障害研究振興基金 美原賞
     
    受賞者: 宝金 清博
  • 2007年09月 北海道医師会賞・北海道知事賞
     
    受賞者: 寳金 清博
  • 1999年04月 日本脳卒中の外科学会賞（鈴木賞）
  • 1993年12月 第22回かなえ医学奨励賞

論文

  • Masahito Kawabori, Satoshi Kuroda, Hideo Shichinohe, Kaoru Kahata, Souichi Shiratori, Satoshi Ikeda, Taisuke Harada, Kenji Hirata, Khin Khin Tha, Masato Aragaki, Shunsuke Terasaka, Yoichi M Ito, Naoki Nishimoto, Shunsuke Ohnishi, Ichiro Yabe, Kohsuke Kudo, Kiyohiro Houkin, Miki Fujimura
    Med (New York, N.Y.) 2024年03月18日 
    BACKGROUND: Ischemic stroke is one of the leading causes of death and neurological disability worldwide, and stem cell therapy is highly expected to reverse the sequelae. This phase 1/2, first-in-human study evaluated the safety, feasibility, and monitoring of an intracerebral-transplanted magnetic resonance imaging (MRI)-trackable autologous bone marrow stromal cell (HUNS001-01) for patients with subacute ischemic stroke. METHODS: The study included adults with severe disability due to ischemic stroke. HUNS001-01 cultured with human platelet lysates and labeled with superparamagnetic iron oxide was stereotactically transplanted into the peri-infarct area 47-64 days after ischemic stroke onset (dose: 2 or 5 × 107 cells). Neurological and radiographic evaluations were performed throughout 1 year after cell transplantation. The trial was registered at UMIN Clinical Trial Registry (number UMIN000026130). FINDINGS: All seven patients who met the inclusion criteria successfully achieved cell expansion, underwent intracerebral transplantation, and completed 1 year of follow-up. No product-related adverse events were observed. The median National Institutes of Health Stroke Scale and modified Rankin scale scores before transplantation were 13 and 4, which showed improvements of 1-8 and 0-2, respectively. Cell tracking proved that the engrafted cells migrated toward the infarction border area 1-6 months after transplantation, and the quantitative susceptibility mapping revealed that cell signals at the migrated area constantly increased throughout the follow-up period up to 34% of that of the initial transplanted site. CONCLUSIONS: Intracerebral transplantation of HUNS001-01 was safe and well tolerated. Cell tracking shed light on the therapeutic mechanisms of intracerebral transplantation. FUNDING: This work was supported by the Japan Agency for Medical Research and Development (AMED; JP17bk0104045 and JP20bk0104011).
  • Kiyohiro Houkin, Toshiya Osanai, Shinichiro Uchiyama, Kazuo Minematsu, Akihiko Taguchi, Katsuhiko Maruichi, Yoshimasa Niiya, Katsuyuki Asaoka, Yoshihiro Kuga, Katsumi Takizawa, Koichi Haraguchi, Shinichi Yoshimura, Kazumi Kimura, Koji Tokunaga, Atsuo Aoyama, Fusao Ikawa, Chikanori Inenaga, Tatsuya Abe, Atsushi Tominaga, Shinichi Takahashi, Kohsuke Kudo, Miki Fujimura, Taku Sugiyama, Masaki Ito, Masahito Kawabori, David C. Hess, Sean I. Savitz, Teruyuki Hirano, Kiyohiro Houkin, Toshiya Osanai, Katsuhiko Maruichi, Yoshimasa Niiya, Katsuyuki Asaoka, Katsumi Takizawa, Kouichi Haraguchi, Rokuya Tanikawa, Akira Tempaku, Yusuke Shimoda, Masanori Isobe, Kenji Kamiyama, Masafumi Ohtaki, Norihito Shimamura, Junta Moroi, Aiki Marushima, Shinichi Takahashi, Takao Urabe, Teruyuki Hirano, Kazumi Kimura, Kazuo Kitagawa, Hidetoshi Kasuya, Yoshikane Izawa, Yasuyuki Iguchi, Koichi Oki, Koichi Kato, Yoshihisa Yamano, Satoshi Kuroda, Atsushi Sato, Chikanori Inenaga, Keizo Yasui, Kazunori Toyoda, Shinichi Yoshimura, Nobuyuki Sakai, Yoshihiro Kuga, Atsuo Aoyama, Fusao Ikawa, Koji Tokunaga, Atsushi Tominaga, Yasushi Takagi, Masahiro Yasaka, Tatsuya Abe, Takayuki Matsuo, Toshiro Yonehara, Tadashi Terasaki, Hideki Matsuoka
    JAMA Neurology 2024年01月16日 
    Importance Cell therapy is a promising treatment approach for stroke and other diseases. However, it is unknown whether MultiStem (HLCM051), a bone marrow–derived, allogeneic, multipotent adult progenitor cell product, has the potential to treat ischemic stroke. Objective To assess the efficacy and safety of MultiStem when administered within 18 to 36 hours of ischemic stroke onset. Design, Setting, and Participants The Treatment Evaluation of Acute Stroke Using Regenerative Cells (TREASURE) multicenter, double-blind, parallel-group, placebo-controlled phase 2/3 randomized clinical trial was conducted at 44 academic and clinical centers in Japan between November 15, 2017, and March 29, 2022. Inclusion criteria were age 20 years or older, presence of acute ischemic stroke (National Institutes of Health Stroke Scale [NIHSS] score of 8-20 at baseline), confirmed acute infarction involving the cerebral cortex and measuring more than 2 cm on the major axis (determined with diffusion-weighted magnetic resonance imaging), and a modified Rankin Scale (mRS) score of 0 or 1 before stroke onset. Data analysis was performed between May 9 and August 15, 2022. Exposure Patients were randomly assigned to either intravenous MultiStem in 1 single unit of 1.2 billion cells or intravenous placebo within 18 to 36 hours of ischemic stroke onset. Main Outcomes and Measures The primary end points were safety and excellent outcome at day 90, measured as a composite of a modified Rankin Scale (mRS) score of 1 or less, a NIHSS score of 1 or less, and a Barthel index score of 95 or greater. The secondary end points were excellent outcome at day 365, mRS score distribution at days 90 and 365, and mRS score of 0 to 1 and 0 to 2 at day 90. Statistical analysis of efficacy was performed using the Cochran-Mantel-Haenszel test. Results This study included 206 patients (104 received MultiStem and 102 received placebo). Their mean age was 76.5 (range, 35-95) years, and more than half of patients were men (112 [54.4%]). There were no between-group differences in primary and secondary end points. The proportion of excellent outcomes at day 90 did not differ significantly between the MultiStem and placebo groups (12 [11.5%] vs 10 [9.8%], P = .90; adjusted risk difference, 0.5% [95% CI, −7.3% to 8.3%]). The frequency of adverse events was similar between treatment groups. Conclusions and Relevance In this randomized clinical trial, intravenous administration of allogeneic cell therapy within 18 to 36 hours of ischemic stroke onset was safe but did not improve short-term outcomes. Further research is needed to determine whether MultiStem therapy for ischemic stroke has a beneficial effect in patients who meet specific criteria, as indicated by the exploratory analyses in this study. Trial Registration ClinicalTrials.gov Identifier: NCT02961504
  • Yuki Takakura, Mika Otsuki, Ryo Takagi, Kiyohiro Houkin
    BMC geriatrics 23 1 575 - 575 2023年09月19日 
    BACKGROUND: The assessment of a wide range of cognitive functions using video teleconference (VTC) systems cannot be applied in practice yet. We aimed to determine the feasibility and reliability of previously unvalidated remote cognitive function tests in Japan using common information and communication technology (ICT) devices, software, and VTC systems compared with face-to-face (FTF) assessment. METHODS: The sample consisted of 26 participants from senior citizens clubs and an employment service centre in Sapporo Japan, including 11 females and 15 males (age averaged 78.6 ± 6.8 years). Tests included the RCPM, Story recall, 10/36 spatial recall, selective reminding test, SDMT, PASAT, FAB, TMT-A, TMT-B, visual cancellation task, digit span, tapping span. The experimental design was a counterbalanced crossover randomised controlled trial. Intraclass correlations (ICCs), paired-samples t-tests, Cohen's Kappa (κ) coefficients, and Wilcoxon signed-rank test were calculated to compare the scores between VTC and FTF assessments. RESULTS: All ICCs were significant and ranged from 0.47 (RCPM time) to 0.92 (RCPM score and PASAT), with a mean ICC of 0.75. Digit span using Cohen's Kappa (κ) coefficient was significant, but the tapping span was not. Paired samples t-test showed statistically significant differences in SDMT, RCPM time, and cancellation time. CONCLUSIONS: The results suggest that remote video conference-based neuropsychological tests even using familiar devices and software may be able to assess a wide range of cognitive functions in the Japanese older population. As for the processing speed tasks, we need to create our own standards for the remote condition. For the tapping span, we should consider increasing the number of trials.
  • Yasuhiro Nishiyama, Kazumi Kimura, Toshiaki Otsuka, Kazunori Toyoda, Shinichiro Uchiyama, Haruhiko Hoshino, Nobuyuki Sakai, Yasushi Okada, Hideki Origasa, Hiroaki Naritomi, Kiyohiro Houkin, Keiji Yamaguchi, Kazuo Minematsu, Masayasu Matsumoto, Teiji Tominaga, Hidekazu Tomimoto, Yasuo Terayama, Satoshi Yasuda, Takenori Yamaguchi
    Stroke 54 3 697 - 705 2023年03月 
    BACKGROUND: The effectiveness of long-term dual antiplatelet therapy (DAPT) to prevent recurrent strokes in patients with lacunar stroke remains unclarified. Therefore, this study aimed to compare and to elucidate the safety and effectiveness of DAPT and single antiplatelet therapy (SAPT) in preventing recurrence in chronic lacunar stroke. METHODS: CSPS.com (Cilostazol Stroke Prevention Study for Antiplatelet Combination) was a prospective, multicenter, randomized controlled trial. In this prespecified subanalysis, 925 patients (mean age, 69.5 years; 69.4% men) with lacunar stroke were selected from 1884 patients with high-risk noncardioembolic stroke, enrolled in the CSPS.com trial after 8 to 180 days following stroke. Patients were randomly assigned to receive either SAPT or DAPT using cilostazol and were followed for 0.5 to 3.5 years. The primary efficacy outcome was the first recurrence of ischemic stroke. The safety outcomes were severe or life-threatening bleeding. RESULTS: The DAPT group receiving cilostazol and either aspirin or clopidogrel and SAPT group receiving aspirin or clopidogrel alone comprised 464 (50.2%) and 461 (49.8%) patients, respectively. Ischemic stroke occurred in 12 of 464 patients (1.84 per 100 patient-years) in the DAPT group and 31 of 461 patients (4.42 per 100 patient-years) in the SAPT group, during follow-up. After adjusting for multiple potential confounding factors, ischemic stroke risk was significantly lower in the DAPT group than in the SAPT group (hazard ratio, 0.43 [95% CI, 0.22-0.84]). The rate of severe or life-threatening hemorrhage did not differ significantly between the groups (2 patients [0.31 per 100 patient-years] versus 6 patients [0.86 per 100 patient-years] in the DAPT and SAPT groups, respectively; hazard ratio, 0.36 [95% CI, 0.07-1.81]). CONCLUSIONS: In patients with lacunar stroke, DAPT using cilostazol had significant benefits in reducing recurrent ischemic stroke incidence compared with SAPT without increasing the risk of severe or life-threatening bleeding. REGISTRATION: URL: https://www. CLINICALTRIALS: gov; Unique identifier: NCT01995370. URL: https://www.umin.ac.jp/ctr; Unique identifier: UMIN000012180.
  • Yasuhiro Morii, Kagari Abiko, Toshiya Osanai, Jiro Takami, Takumi Tanikawa, Kensuke Fujiwara, Kiyohiro Houkin, Katsuhiko Ogasawara
    Cost effectiveness and resource allocation : C/E 21 1 12 - 12 2023年02月01日 
    BACKGROUND: Rehabilitation is an essential medical service for patients who have suffered acute stroke. Although the effectiveness of 7-days-per-week rehabilitation schedule has been studied in comparison with 5- or 6-days-per-week rehabilitation schedule, its cost-effectiveness has not been analyzed. In this research, to help formulate more cost-effective medical treatments for acute stroke patients, we analyzed the cost-effectiveness of 7-days-per-week rehabilitation for acute stroke from public health payer's perspective, and public healthcare and long-term care payer's perspective in Japan. METHODS: Cost-effectiveness of 7-days-per-week rehabilitation for acute stroke patients was analyzed based on the result from a previous study using a Japanese database examining the efficacy of 7-days-per-week rehabilitation. Cost utility analysis was conducted by comparing 7-days-per-week rehabilitation with 5- or 6-days-per-week rehabilitation, with its main outcome incremental cost-effectiveness ratio (ICER) calculated by dividing estimated incremental medical and long-term care costs by incremental quality-adjusted life years (QALY). The costs were estimated using the Japanese fee table and from published sources. The time horizon was 5 years, and Markov modeling was used for the analysis. RESULTS: The ICER was $6339/QALY from public health payer's perspective, lower than 5,000,000 Yen/QALY (approximately US$37,913), which was the willingness-to-pay used for the cost-effectiveness evaluation in Japan. The 7-day-per-week rehabilitation was dominant from public healthcare and long-term care payer's perspective. The result of sensitivity analysis confirmed the results. CONCLUSION: The results indicated that 7-days-per-week rehabilitation for acute stroke rehabilitation was likely to be cost-effective.
  • 高倉 祐樹, 大槻 美佳, 高木 諒, 寳金 清博
    老年精神医学雑誌 33 増刊II 356 - 356 (株)ワールドプランニング 2022年11月
  • 高齢者における脳トレアプリの効果的な使用条件の検討 PROBE法での無作為化比較試験
    高倉 祐樹, 大槻 美佳, 高木 諒, 寳金 清博
    Dementia Japan 36 4 802 - 802 (一社)日本認知症学会 2022年10月
  • Masahito Kawabori, Masaki Ito, Ken Kazumata, Kikutaro Tokairin, Kanako C. Hatanaka, Susumu Ishikawa, Kiyohiro Houkin, Miki Fujimura
    Cerebrovascular Diseases 52 2 171 - 176 2022年09月05日 
    Background: Superficial temporal artery (direct) and encephalomyosynangiosis (indirect) revascularization may develop after combined bypass surgery in pediatric patients with moyamoya disease (MMD). However, arterial development varies widely among patients, and the underlying mechanisms remain unknown. Objectives: We evaluated the relationship between the development of donor arteries after bypass surgery in pediatric patients with MMD and the MMD-susceptibility gene variant c.14576G>A of ring finger protein (RNF) 213. Methods: The data of pediatric patients with MMD (age <16 years at the time of surgery) treated with combined bypass surgery between September 2013 and April 2019 were consecutively analyzed. Quantitative measurements of the superficial temporal artery (STA), deep temporal artery (DTA), and middle meningeal artery (MMA) diameters with magnetic resonance angiography (MRA) source imaging were performed preoperatively and at 6–12 months postoperatively. The postoperative caliber change ratios (CCRs) were calculated. The relationship between CCRs and RNF213 c.14576G>A status was examined. Results: Forty-eight hemispheres from 28 pediatric patients with MMD were examined. Three hemispheres belonged to patients with the AA genotype; 33 to patients with the AG genotype (AA/AG group); and 12 to patients with the GG genotype (GG group; wild type). The CCRs for the DTA were significantly higher in patients with RNF213 variant (AA/AG group; 2.5 ± 0.1) than in the GG group (2.0 ± 0.2) (p = 0.03), whereas the CCRs for the STA were significantly higher in the GG (1.6 ± 0.1) than in the AA/AG group (1.3 ± 0.6) (p = 0.02). There was no significant difference in the CCRs for the MMA and basilar artery between the groups. Other factors, including sex, age, and MRA grading, were not associated with the development of specific bypass development. Conclusions: The extent of collateral development associated with direct or indirect bypass was found to differ between the genotypes of the RNF213 c.14576G>A associated with pediatric MMD. This genetic variant correlates with the development of the disease and affects revascularization after bypass surgery in pediatric patients with MMD.
  • 脳深部刺激療法術後4年の経過に関する12例の検討
    白井 慎一, 江口 克紀, 山崎 和義, 松島 理明, 加納 崇裕, 笹森 徹, 平田 健司, 大槻 美佳, 北川 まゆみ, 寳金 清博, 佐々木 秀直, 藤村 幹, 矢部 一郎
    パーキンソン病・運動障害疾患コングレスプログラム・抄録集 16回 80 - 80 Movement Disorder Society of Japan (MDSJ) 2022年07月
  • 脳深部刺激療法術後4年の経過に関する12例の検討
    白井 慎一, 江口 克紀, 山崎 和義, 松島 理明, 加納 崇裕, 笹森 徹, 平田 健司, 大槻 美佳, 北川 まゆみ, 寳金 清博, 佐々木 秀直, 藤村 幹, 矢部 一郎
    パーキンソン病・運動障害疾患コングレスプログラム・抄録集 16回 80 - 80 Movement Disorder Society of Japan (MDSJ) 2022年07月
  • Yukitomo Ishi, Takumi Era, Sayaka Yuzawa, Michinari Okamoto, Ryosuke Sawaya, Hiroaki Motegi, Shigeru Yamaguchi, Shunsuke Terasaka, Kiyohiro Houkin, Miki Fujimura
    American journal of medical genetics. Part A 2022年02月18日 
    The diagnosis of mosaicism is challenging in patients with neurofibromatosis type 2 (NF2) subset due to low variant allele frequency. In this study, we generated induced pluripotent stem cells (iPSCs) were generated from a patient clinically diagnosed with NF2 based on multiple schwannomas, including bilateral vestibular schwannomas and meningiomas. Genetic analysis of the patient's mononuclear cells (MNCs) from peripheral blood failed to detect NF2 alteration but successfully found p.Q65X (c.193C>T) mutation in all separate tumors with three intracranial meningiomas and one intraorbital schwannoma, and confirming mosaicism diagnosis in NF2 alteration using deep sequencing. Five different clones with patient-derived iPSCs were established from MNCs in peripheral blood, which showed sufficient expression of pluripotent markers. Genetic analysis showed that one of five generated iPSC lines from MNCs had the same p.Q65X mutation as that found in NF2. There was no significant difference in the expression of genes related to NF2 between iPSC clones with the wild-type and mutant NF2. In this case, clonal expansion of mononuclear bone marrow-derived stem cells recapitulated mosaicism's genetic alteration in NF2. Patient-derived iPSCs from mosaic NF2 would contribute to further functional research of NF2 alteration.
  • Masaki Ito, Masahito Kawabori, Taku Sugiyama, Kikutaro Tokairin, Ryota Tatezawa, Haruto Uchino, Ken Kazumata, Kiyohiro Houkin, Miki Fujimura
    Neurosurgical review 45 3 2305 - 2313 2022年02月07日 
    Direct superficial temporal artery (STA) to middle cerebral artery (MCA) anastomosis combined with indirect pial synangiosis provides favorable surgical collaterals for Moyamoya disease (MMD), especially in adults; however, factors leading to the development of each direct and indirect collateral are not well documented.We aimed to investigate the association between RNF213 founder polymorphism (p.R4810K) and each direct and indirect collateral development. By qualitative and quantitative evaluations of direct and indirect surgical collaterals using time-of-flight MR angiography, postoperative development of each type of bypass was evaluated independently into two categories. Multivariate logistic regression analysis was performed to study the contributing factors for the development of each surgical collateral. Excellent development of postoperative direct and indirect bypass was observed in 65 hemispheres (70%) by qualitative evaluation, which was confirmed by quantitative evaluation. Multivariate logistic regression analysis of excellent indirect bypass development revealed a significant positive correlation with the p.R4810K (odds ratio, OR4.0; 95%-confidence interval, CI 1.2-16), advanced MR angiographic stage (OR9.5; 95%CI 1.7-73), and preoperative middle meningeal artery caliber (OR6.8; 95%CI 1.8-35), but a significant negative correlation was found with the excellent direct bypass development (OR0.17; 95%CI 0.03-0.75). No significant correlation was observed between excellent direct bypass development and the p.R4810K (OR0.95; 95%CI 0.37-2.4).In conclusion, excellent development of indirect collaterals after STA-MCA anastomosis combined with indirect pial synangiosis occurs more frequently in adult MMD with the RNF213 founder polymorphism, suggesting a role of the p.R4810K variant for marked in-growth of indirect collaterals and the utility of preoperative genetic analysis.
  • Yukitomo Ishi, Shigeru Yamaguchi, Kanako C Hatanaka, Emi Takakuwa, Hiroaki Motegi, Taishi Honda, Hiroyuki Kobayashi, Shunsuke Terasaka, Akihiro Homma, Miki Fujimura, Kiyohiro Houkin
    Acta medica Okayama 76 1 93 - 98 2022年02月 
    A 66-year-old man underwent multimodal treatment for olfactory neuroblastoma (ONB). When he was 72 years old, a cystic intracranial lesion without accumulation on fluorine-18-fluorodeoxyglucose positron emission tomography was detected. Surgical resection was performed when the patient was 73 years old. The pathological examination revealed recurrence of ONB, and the patient underwent focal irradiation. At age 81, he presented with a second recurrence in the right occipital lobe with radiological and pathological findings similar to the prior recurrence. This case suggests that pathological confirmation should be considered in cases with atypical radiological findings following the treatment of ONB.
  • Soichiro Takamiya, Masahito Kawabori, Kazuyoshi Yamazaki, Sho Yamaguchi, Aki Tanimori, Koji Yamamoto, Shunsuke Ohnishi, Toshitaka Seki, Kotaro Konno, Khin Khin Tha, Daigo Hashimoto, Masahiko Watanabe, Kiyohiro Houkin, Miki Fujimura
    PloS one 17 7 e0270606  2022年 
    Spinal cord injury (SCI) is often accompanied by gastrointestinal dysfunction due to the disconnection of the spinal autonomic nervous system. Gastrointestinal dysfunction reportedly upregulates intestinal permeability, leading to bacterial translocation of the gut microbiome to the systemic circulation, which further activates systemic inflammation, exacerbating neuronal damage. Mesenchymal stem cells (MSC) reportedly ameliorate SCI. Here, we aimed to investigate their effect on the associated gastrointestinal dysfunction. Human amnion-derived MSC (AMSCs) were intravenously transplanted one day after a rat model of midthoracic SCI. Biodistribution of transplanted cells, behavioral assessment, and histological evaluations of the spinal cord and intestine were conducted to elucidate the therapeutic effect of AMSCs. Bacterial translocation of the gut microbiome was examined by in situ hybridization and bacterial culture of the liver. Systemic inflammations were examined by blood cytokines, infiltrating immune cells in the spinal cord, and the size of the peripheral immune tissue. AMSCs released various neurotrophic factors and were mainly distributed in the liver and lung after transplantation. AMSC-transplanted animals showed smaller spinal damage and better neurological recovery with preserved neuronal tract. AMSCs transplantation ameliorated intestinal dysfunction both morphologically and functionally, which prevented translocation of the gut microbiome to the systemic circulation. Systemic inflammations were decreased in animals receiving AMSCs in the chronic phase. Intravenous AMSC administration during the acute phase of SCI rescues both spinal damage and intestinal dysfunction. Reducing bacterial translocation may contribute to decreasing systemic inflammation.
  • Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Michinari Okamoto, Hiroyuki Kobayashi, Kenji Hirata, Yoshitaka Oda, Shinya Tanaka, Shunsuke Terasaka, Kiyohiro Houkin
    Journal of Neurosurgical Sciences 64 6 502 - 508 2021年12月01日 
    BACKGROUND: Although newly diagnosed high-grade glioma patients in Japan can receive bevacizumab (BEV) as first-line chemotherapy, randomized clinical trials have not shown a survival benefit for BEV for these patients. In this study, we investigated whether selective add-on BEV for patients with newly diagnosed glioblastoma (GBM) and anaplastic astrocytoma (AA) improves prognosis, in cases where tumors were continuously growing during radiotherapy concomitant with temozolomide (TMZ). METHODS: We conducted a retrospective survey of the overall survival (OS) of patients with GBM/AAs who were treated in our institution between 2006 and 2016. Patients whose tumors were continuously growing regardless of radiotherapy were categorized as the "progressive"group remaining patients were categorized as the "non-progressive"group. Since 2013, patients in the "progressive"group received add-on BEV therapy with the Stupp regimen during or just after radiotherapy. RESULTS: Of 151 GBM/AApatients, 34 (22.5%) were categorized in the "progressive"group. Median OSs of the "progressive"and "non-progressive"groups were 13.2 months and 25.3 months, respectively (P< 0.001). Twelve patients in the "progressive"group received add-on BEV therapy, and their median OSwas 20.2 months whereas for the remaining 22 patients in the "progressive"group who were treated before the BEV era, their median OS was 10.5 months. In the "progressive"group, add-on BEV significantly extended OS (P=0.018) and was the lone clinical factor of better prognosis. CONCLUSIONS: We found that, for patients with GBM/AAs whose tumors were continuously growing during radiotherapy, add-on BEV treata ment resulted in survival benefits.
  • 脳血管障害の病態評価における脳画像の有用性 もやもや病の周術期画像マーカーとRNF213遺伝子多型の関連
    内野 晴登, 伊東 雅樹, 東海林 菊太郎, 舘澤 諒大, 川堀 真人, 数又 研, 宝金 清博, 藤村 幹
    脳循環代謝 33 1 70 - 70 (一社)日本脳循環代謝学会 2021年11月
  • RNF213創始者変異は成人もやもや病複合血行再建術後の間接バイパス路発達に寄与する
    伊東 雅基, 川堀 真人, 杉山 拓, 東海林 菊太郎, 舘澤 諒大, 内野 晴登, 数又 研, 寳金 清博, 藤村 幹
    脳循環代謝 33 1 94 - 94 (一社)日本脳循環代謝学会 2021年11月
  • RNF213創始者変異は成人もやもや病複合血行再建術後の間接バイパス路発達に寄与する
    伊東 雅基, 川堀 真人, 杉山 拓, 東海林 菊太郎, 舘澤 諒大, 内野 晴登, 数又 研, 寳金 清博, 藤村 幹
    脳循環代謝 33 1 94 - 94 (一社)日本脳循環代謝学会 2021年11月
  • Shinichiro Uchiyama, Kazunori Toyoda, Katsuhiro Omae, Ryotaro Saita, Kazumi Kimura, Haruhiko Hoshino, Nobuyuki Sakai, Yasushi Okada, Kortaro Tanaka, Hideki Origasa, Hiroaki Naritomi, Kiyohiro Houkin, Keiji Yamaguchi, Masanori Isobe, Kazuo Minematsu, Masayasu Matsumoto, Teiji Tominaga, Hidekazu Tomimoto, Yasuo Terayama, Satoshi Yasuda, Takenori Yamaguchi
    Journal of the American Heart Association 10 20 e022575  2021年10月19日 [査読有り]
     
    Background Long-term benefit of dual antiplatelet therapy (DAPT) over single antiplatelet therapy (SAPT) for the prevention of recurrent stroke has not been established in patients with intracranial arterial stenosis. We compared the efficacy and safety of DAPT with cilostazol and clopidogrel or aspirin to those of SAPT with clopidogrel or aspirin in patients with intracranial arterial stenosis, who were recruited to the Cilostazol Stroke Prevention Study for Antiplatelet Combination trial, a randomized controlled trial in high-risk Japanese patients with ischemic stroke. Methods and Results We compared the vascular and hemorrhagic events between DAPT and SAPT in patients with ischemic stroke and symptomatic or asymptomatic intracranial arterial stenosis of at least 50% in a major intracranial artery. Patients were placed in two groups: 275 were assigned to receive DAPT and 272 patients SAPT. The risks of ischemic stroke (hazard ratio [HR], 0.47; 95% CI, 0.23-0.95); and composite of stroke, myocardial infarction, and vascular death (HR, 0.48; 95% CI, 0.26-0.91) were lower in DAPT than SAPT, whereas the risk of severe or life-threatening bleeding (HR, 0.72; 95% CI, 0.12-4.30) did not differ between the 2 treatment groups. Conclusions DAPT using cilostazol was superior to SAPT with clopidogrel or aspirin for the prevention of recurrent stroke and vascular events without increasing bleeding risk among patients with intracranial arterial stenosis after stroke. Registration URL: https://www.clinicaltrials.gov; Unique identifier: NCT01995370.
  • 妄想対象が「死別した近親者」ではない養生(nurturing)行動の発現機序
    高倉 祐樹, 大槻 美佳, 中川 賀嗣, 寳金 清博
    Dementia Japan 35 4 635 - 635 (一社)日本認知症学会 2021年10月
  • Masayuki Gekka, Toshiya Osanai, Takeshi Aoki, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin, Miki Fujimura
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 30 10 106007 - 106007 2021年10月 [査読有り]
     
    OBJECTIVES: During carotid artery stenting (CAS), hemodynamics may be affected by the carotid sinus reflex in some cases. Although general anesthesia has been reported to stabilize intraoperative hemodynamics, the patient's neurological condition must be assessed indirectly. Therefore, we investigated the changes in intraoperative hemodynamics and perioperative complications of CAS under general anesthesia and evaluated the efficacy of somatosensory evoked potential (SEP) monitoring in detecting a reduction in perfusion during CAS. MATERIALS AND METHODS: From April 2011 to August 2016,57 consecutive patients who underwent CAS under general anesthesia were evaluated. The follow-up period ranged from 3 to 8 years. During CAS, anesthesiologists monitored and managed the hemodynamics. SEP monitoring was performed during the CAS procedure in all patients. RESULTS: Intraoperative hypotension (systolic blood pressure ≤ 100 mmHg) was evident in 16 patients (28.1%), and 13 patients (22.8%) experienced intraoperative bradycardia; however, all of these cases were promptly managed under general anesthesia. None of the patients showed systolic blood pressure < 50 mmHg from baseline. Regarding perioperative complications, none of the patients exhibited myocardial infarction or postoperative hyperperfusion symptoms, and there was no mortality. Among 21 patients (36.8%) with a decrease in the intraoperative SEP, 3 (5.3%) exhibited transient ischemic symptoms and 1 (1.8%) had postoperative infarction. CONCLUSIONS: CAS under general anesthesia is a safe and effective management option in terms of intraoperative hemodynamic stability. In addition, our findings indicate that SEP monitoring could be helpful in evaluating transient postoperative cerebral ischemia or cerebral infarction after CAS.
  • Katsuki Eguchi, Shinichi Shirai, Masaaki Matsushima, Takahiro Kano, Kazuyoshi Yamazaki, Shuji Hamauchi, Toru Sasamori, Toshitaka Seki, Kenji Hirata, Mayumi Kitagawa, Mika Otsuki, Tohru Shiga, Kiyohiro Houkin, Hidenao Sasaki, Ichiro Yabe
    BMC neurology 21 1 351 - 351 2021年09月13日 [査読有り]
     
    BACKGROUND: Weight gain (WG) is a frequently reported side effect of subthalamic deep brain stimulation; however, the underlying mechanisms remain unclear. The active contact locations influence the clinical outcomes of subthalamic deep brain stimulation, but it is unclear whether WG is directly associated with the active contact locations. We aimed to determine whether WG is associated with the subthalamic deep brain stimulation active contact locations. METHODS: We enrolled 14 patients with Parkinson's disease who underwent bilateral subthalamic deep brain stimulation between 2013 and 2019. Bodyweight and body mass index were measured before and one year following the surgery. The Lead-DBS Matlab toolbox was used to determine the active contact locations based on magnetic resonance imaging and computed tomography. We also created sweet spot maps for WG using voxel-wise statistics, based on volume of tissue activation and the WG of each patient. Fluorodeoxyglucose-positron emission tomography data were also acquired before and one year following surgery, and statistical parametric mapping was used to evaluate changes in brain metabolism. We examined which brain regions' metabolism fluctuation significantly correlated with increased body mass index scores and positron emission tomography data. RESULTS: One year after surgery, the body mass index increase was 2.03 kg/m2. The sweet spots for WG were bilateral, mainly located dorsally outside of the subthalamic nucleus (STN). Furthermore, WG was correlated with increased metabolism in the left limbic and associative regions, including the middle temporal gyrus, inferior frontal gyrus, and orbital gyrus. CONCLUSIONS: Although the mechanisms underlying WG following subthalamic deep brain stimulation are possibly multifactorial, our findings suggest that dorsal stimulation outside of STN may lead to WG. The metabolic changes in limbic and associative cortical regions after STN-DBS may also be one of the mechanisms underlying WG. Further studies are warranted to confirm whether dorsal stimulation outside of STN changes the activities of these cortical regions.
  • Yohei Mineharu, Yasushi Takagi, Akio Koizumi, Takaaki Morimoto, Takeshi Funaki, Tomohito Hishikawa, Yoshio Araki, Hitoshi Hasegawa, Jun C Takahashi, Satoshi Kuroda, Kiyohiro Houkin, Susumu Miyamoto
    Journal of neurosurgery 136 4 1 - 10 2021年09月10日 [査読有り]
     
    OBJECTIVE: Although many studies have analyzed risk factors for contralateral progression in unilateral moyamoya disease, they have not been fully elucidated. The aim of this study was to examine whether genetic factors as well as nongenetic factors are involved in the contralateral progression. METHODS: The authors performed a multicenter cohort study in which 93 cases with unilateral moyamoya disease were retrospectively reviewed. The demographic features, RNF213 R4810K mutation, lifestyle factors such as smoking and drinking, past medical history, and angiographic findings were analyzed. A Cox proportional hazards model was used to find risk factors for contralateral progression. RESULTS: Contralateral progression was observed in 24.7% of cases during a mean follow-up period of 72.2 months. Clinical characteristics were not significantly different between 63 patients with the R4810K mutation and those without it. Cox regression analysis showed that the R4810K mutation (hazard ratio [HR] 4.64, p = 0.044), childhood onset (HR 7.21, p < 0.001), male sex (HR 2.85, p = 0.023), and daily alcohol drinking (HR 4.25, p = 0.034) were independent risk factors for contralateral progression. CONCLUSIONS: These results indicate that both genetic and nongenetic factors are associated with contralateral progression of unilateral moyamoya disease. The findings would serve to help us better understand the pathophysiology of moyamoya disease and to manage patients more appropriately.
  • Haruhiko Hoshino, Kazunori Toyoda, Katsuhiro Omae, Noriyuki Ishida, Shinichiro Uchiyama, Kazumi Kimura, Nobuyuki Sakai, Yasushi Okada, Kortaro Tanaka, Hideki Origasa, Hiroaki Naritomi, Kiyohiro Houkin, Keiji Yamaguchi, Masanori Isobe, Kazuo Minematsu, Masayasu Matsumoto, Teiji Tominaga, Hidekazu Tomimoto, Yasuo Terayama, Satoshi Yasuda, Takenori Yamaguchi
    Stroke 52 11 STROKEAHA121034378 - 3439 2021年08月18日 [査読有り]
     
    BACKGROUND AND PURPOSE: Although dual antiplatelet therapy (DAPT) with aspirin and clopidogrel reduces the recurrence of ischemic stroke while significantly increasing the bleeding events compared with monotherapy, the CSPS.com trial (Cilostazol Stroke Prevention Study combination) showed that DAPT using cilostazol was more effective without the bleeding risk. In the CSPS.com trial, aspirin or clopidogrel was used as the underlying antiplatelet drug. The effectiveness and safety of each combination were examined and clarified. METHODS: In the CSPS.com trial, a multicenter, open-label, randomized controlled study, patients with high-risk, noncardioembolic ischemic stroke 8 to 180 days after onset treated with aspirin or clopidogrel alone at the discretion of the physician in charge were recruited. Patients were randomly assigned to receive either monotherapy or DAPT using cilostazol and followed for 0.5 to 3.5 years. The primary efficacy outcome was first recurrence of ischemic stroke. The safety outcome was severe or life-threatening bleeding. The analysis was based on the underlying antiplatelet agents. RESULTS: A total of 763 patients taking aspirin and 1116 taking clopidogrel were included in the intention-to-treat analysis. Although the clopidogrel group had more risk factors than the aspirin group, the primary efficacy outcome and safety outcome did not differ significantly between the 2 groups. In the aspirin group, the primary efficacy outcome and safety outcome did not differ significantly between the DAPT group and the aspirin-monotherapy group. In the clopidogrel group, the primary end point occurred at a rate of 2.31 per 100 patient-years in the DAPT group and 5.19 per 100 patient-years in the clopidogrel-monotherapy group (hazard ratio, 0.447 [95% CI, 0.258-0.774]). Safety outcome did not differ significantly between groups (0.51 per 100 patient-years versus 0.71 per 100 patient-years, respectively; hazard ratio, 0.730 [95% CI, 0.206-2.588]). CONCLUSIONS: The combination of cilostazol and clopidogrel significantly reduced the recurrence of ischemic stroke without increasing the bleeding risk in noncardioembolic, high-risk patients. REGISTRATION: URL: http://www.clinicaltrials.gov; Unique identifier: NCT01995370. URL: https://www.umin.ac.jp/ctr/; Unique identifier: UMIN000012180.
  • Yasuhiro Morii, Toshiya Osanai, Kensuke Fujiwara, Takumi Tanikawa, Kiyohiro Houkin, Songzi Gu, Katsuhiko Ogasawara
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 30 8 105843 - 105843 2021年08月 [査読有り]
     
    OBJECTIVES: There are regional disparities in implementation rates of endovascular thrombectomy due to time and resource constraints such as endovascular thrombectomy specialists. In Hokkaido, Japan, Drive and Retrieve System (DRS), where endovascular thrombectomy specialists perform early endovascular thrombectomies by traveling from the facilities where they normally work to facilities closer to the patient. This study analyzed the cost-effectiveness of allocating a endovascular thrombectomy specialist for DRS to treat stroke patients. MATERIALS AND METHODS: he number of ischemic stroke patients expected to receive endovascular thrombectomy in Hokkaido in 2015 was estimated. It was assumed that an additional neutointerventionist was allocated for DRS. The analysis was performed from the government's perspective, which includes medical and nursing-care costs, and the personnel cost for endovascular thrombectomy specialist. The analysis was conducted comparing the current scenario, where patients received endovascular thrombectomy in facilities where endovascular thrombectomy specialists normally work, with the scenario with DRS within 60 min drive distance. Patient transport time was analyzed using geographic information system, and patient severity was estimated from the transport time. The primary outcome was incremental cost-effectiveness ratio (ICER) in each medical area which was calculated from the incremental costs and the incremental quality-adjusted life years (QALYs), estimated from patient severity using published literature. The entire process was repeated 100 times. RESULTS: DRS was most cost-effective in Kamikawachubu area, where the ICER was $14,173±16,802/QALY, significantly lower than the threshold that the Japanese guideline suggested. CONCLUSIONS: Since DRS was cost-effective in Kamikawachubu area, the area should be prioritized when a endovascular thrombectomy specialist for DRS is allocated as a policy.
  • Katsuki Eguchi, Shinichi Shirai, Masaaki Matsushima, Takahiro Kano, Tomohiro Ichikawa, Kazuyoshi Yamazaki, Shuji Hamauchi, Toru Sasamori, Toshitaka Seki, Mayumi Kitagawa, Hideaki Shiraishi, Kiyohiro Houkin, Hidenao Sasaki, Ichiro Yabe
    Parkinsonism & related disorders 89 148 - 150 2021年08月 [査読有り]
     
    We compared β-γ phase amplitude coupling (PAC) before and one year after chronic deep brain stimulation (DBS) in patients with Parkinson's disease using EEG and observed significant post-operative reduction of PAC values. Our findings suggest that the reduction in PAC due to DBS can be observed after chronic stimulation, which is not a transient phenomenon just after the start of DBS.
  • Shigeru Yamaguchi, Hiroaki Motegi, Yukitomo Ishi, Michinari Okamoto, Ryosuke Sawaya, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
    Neurologia medico-chirurgica 61 4 245 - 252 2021年04月15日 [査読有り]
     
    Bevacizumab (BEV) is a key anti-angiogenic agent used in the treatment for recurrent glioblastoma multiforme (GBM). The aim of this study was to investigate whether cytoreductive surgery prior to treatment with BEV contributes to prolongation of survival for patients with recurrent GBM. We retrospectively analyzed the treatment outcomes of 124 patients with recurrent GBM who were initially treated with the Stupp protocol between 2006 and 2019. Given that BEV has only been available in Japan since 2013, we grouped the patients into two groups according to the time of first recurrence: the pre-BEV group (N = 51) included patients who had recurrence before BEV approval, and the BEV group (N = 73) included patients with recurrence after BEV approval. The overall survival after first recurrence (OS-R) was analyzed according to the treatment strategy. Among 124 patients, 27 patients (19.4%) received cytoreductive surgery. There were nine cases in the pre-BEV group and 18 cases in the BEV group. Although the mean extent of resection for both groups was almost equal, OS-R was significantly different. The median OS-R was 8.1 m in the pre-BEV group and 16.3 m in the BEV group (P = 0.007). Multivariate analysis revealed that the unavailability of BEV postoperatively (P = 0.03) and decreasing performance status by surgery (P = 0.01) were significant poor prognostic factors for survival after surgery. With the advent of BEV, cytoreductive surgery might provide superior survival benefit at the time of GBM recurrence, especially in cases where surgery can be performed without deteriorating the patient's condition.
  • Masahito Kawabori, Hideo Shichinohe, Satoshi Kuroda, Kiyohiro Houkin
    STROKE 52 2021年03月 [査読有り]
  • Hiroaki Motegi, Shigeru Yamaguchi, Michinari Okamoto, Yukitomo Ishi, Shogo Endo, Sadahiro Kaneko, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
    INTERDISCIPLINARY NEUROSURGERY-ADVANCED TECHNIQUES AND CASE MANAGEMENT 23 2021年03月 [査読有り]
     
    Objective: Lumbar drains (LD) have been identified to cause brain sag (BS), which is a serious intracranial hypotension that causes coma; however, other contributing risk factors should also be considered. Therefore, this study aims to identify the BS risk factors and determine the proper treatment plan after brain tumor surgery for patients with BS.Methods: This retrospective study included patients who underwent brain tumor surgery between 2011 and 2015. BS was diagnosed based on the clinical signs of cerebrospinal fluid hypovolemia along with the radiological findings of the basal cistern effacement. Based on the data on the clinical features, the symptoms were used to determine the BS risk factors using logistic regression.Results: Overall, among the 412 patients included in this study, 12 (2.91%) were found to develop BS, 10 experienced altered consciousness, and 2 experienced orthostatic headaches. In all cases, radiological images at onset showed epidural/subdural fluid collection, along with basal cistern effacement. LD was inserted just before surgery in 11 cases, while a ventriculoperitoneal shunt was placed in one patient. The treatment strategies used are as follows: Ringer's solution infusion (n = 4), Trendelenburg position (n = 2), intrathecal injection of saline (n = 1), or no care due to misdiagnosis (n = 5). Moreover, the significant BS risk factors were determined to be the LD (odds ratio [OR] 30.70, CI 2.77-339.86), supratentorial skull base approach (SBA) (OR 35.71, CI 9.1922-138.7250), surgical time (1693.07, CI 69.5621-41207.448), and postoperative hyperosmotic diuretic use (OR 8.092, CI 2.485-26.345) after surgery.Conclusions: In the brain tumor surgery, aside from the LD, the application of supratentorial SBA, use of HD, and lengthy surgery are also found to be contributing risk factors for BS. Therefore, BS should be considered in the differential diagnosis of high-risk patients with postoperative altered consciousness.
  • Taku Sugiyama, Tod Clapp, Jordan Nelson, Chad Eitel, Hiroaki Motegi, Naoki Nakayama, Tsukasa Sasaki, Kikutaro Tokairin, Masaki Ito, Ken Kazumata, Kiyohiro Houkin
    Operative neurosurgery (Hagerstown, Md.) 20 3 289 - 299 2021年02月16日 [査読有り]
     
    BACKGROUND: Adequate surgical planning includes a precise understanding of patient-specific anatomy and is a necessity for neurosurgeons. Although the use of virtual reality (VR) technology is emerging in surgical planning and education, few studies have examined the effectiveness of immersive VR during surgical planning using a modern head-mounted display. OBJECTIVE: To investigate if and how immersive VR aids presurgical discussions of cerebrovascular surgery. METHODS: A multiuser immersive VR system, BananaVisionTM, was developed and used during presurgical discussions in a prospective patient cohort undergoing cerebrovascular surgery. A questionnaire/interview was administered to multiple surgeons after the surgeries to evaluate the effectiveness of the VR system compared to conventional imaging modalities. An objective assessment of the surgeon's knowledge of patient-specific anatomy was also conducted by rating surgeons' hand-drawn presurgical illustrations. RESULTS: The VR session effectively enhanced surgeons' understanding of patient-specific anatomy in the majority of cases (83.3%). An objective assessment of surgeons' presurgical illustrations was consistent with this result. The VR session also effectively improved the decision-making process regarding minor surgical techniques in 61.1% of cases and even aided surgeons in making critical surgical decisions about cases involving complex and challenging anatomy. The utility of the VR system was rated significantly higher by trainees than by experts. CONCLUSION: Although rated as more useful by trainees than by experts, immersive 3D VR modeling increased surgeons' understanding of patient-specific anatomy and improved surgical strategy in certain cases involving challenging anatomy.
  • Muneaki Matsuo, Satomi Nadanaka, Minami Soga, Taku Sugiyama, Shota Serigano, Kenjiro Shimano, Fumio Ichinose, Takuji Nakamura, Toshiyuki Maeda, Kiyohiro Houkin, Takumi Era, Hiroshi Kitagawa
    Scientific reports 11 1 1552 - 1552 2021年01月15日 [査読有り]
     
    Moyamoya disease (MMD) is characterized by progressive bilateral stenotic changes in the terminal portion of the internal carotid arteries. Although RNF213 was identified as a susceptibility gene for MMD, the exact pathogenesis remains unknown. Immunohistochemical analysis of autopsy specimens from a patient with MMD revealed marked accumulation of hyaluronan and chondroitin sulfate (CS) in the thickened intima of occlusive lesions of MMD. Hyaluronan synthase 2 was strongly expressed in endothelial progenitor cells in the thickened intima. Furthermore, MMD lesions showed minimal staining for CS and hyaluronan in the endothelium, in contrast to control endothelium showing positive staining for both. Glycosaminoglycans of endothelial cells derived from MMD and control induced pluripotent stem cells demonstrated a decreased amount of CS, especially sulfated CS, in MMD. A computational fluid dynamics model showed highest wall shear stress values in the terminal portion of the internal carotid artery, which is the predisposing region in MMD. Because the peri-endothelial extracellular matrix plays an important role in protection, cell adhesion and migration, an altered peri-endothelial matrix in MMD may contribute to endothelial vulnerability to wall shear stress. Invading endothelial progenitor cells repairing endothelial injury would produce excessive hyaluronan and CS in the intima, and cause vascular stenosis.
  • 慢性期頭部外傷患者に対する間葉系幹細胞(SB623)脳内投与の治験成績(STEMTRA研究)
    川堀 真人, 今井 英明, 安原 隆雄, 末永 潤, 中村 元, 唐沢 康暉, 宝金 清博
    日本脳神経外傷学会プログラム・抄録集 44回 59 - 59 (一社)日本脳神経外傷学会 2021年01月
  • Koji IIHARA, Nobuhito SAITO, Michiyasu SUZUKI, Isao DATE, Yukihiko FUJII, Kiyohiro HOUKIN, Tooru INOUE, Toru IWAMA, Takakazu KAWAMATA, Phyo KIM, Hiroyuki KINOUCHI, Haruhiko KISHIMA, Eiji KOHMURA, Kaoru KURISU, Keisuke MARUYAMA, Yuji MATSUMARU, Nobuhiro MIKUNI, Susumu MIYAMOTO, Akio MORITA, Hiroyuki NAKASE, Yoshitaka NARITA, Ryo NISHIKAWA, Kazuhiko NOZAKI, Kuniaki OGASAWARA, Kenji OHATA, Nobuyuki SAKAI, Hiroaki SAKAMOTO, Yoshiaki SHIOKAWA, Jun C. TAKAHASHI, Keisuke UEKI, Toshihiko WAKABAYASHI, Koji YOSHIMOTO, Hajime ARAI, Teiji TOMINAGA
    Neurologia medico-chirurgica 2021年
  • Masahito Kawabori, Aki Tanimori, Shinri Kitta, Hideo Shichinohe, Kiyohiro Houkin
    Stem cells international 2021 9796010 - 9796010 2021年 [査読有り]
     
    [This corrects the article DOI: 10.1155/2020/4085617.].
  • Kazuyoshi Yamazaki, Masahito Kawabori, Toshitaka Seki, Soichiro Takamiya, Kotaro Konno, Masahiko Watanabe, Kiyohiro Houkin, Miki Fujimura
    Stem cells international 2021 9964877 - 9964877 2021年 [査読有り]
     
    Stem cell therapy has been shown to reverse the sequelae of spinal cord injury (SCI). Although the ideal treatment route remains unknown, providing a large number of stem cells to the injured site using less invasive techniques is critical to achieving maximal recovery. This study was conducted to determine whether administration of bone marrow stem cell (BMSC) sheet made on its own without a scaffold is superior to intramedullary cell transplantation in a rat subacute SCI model. Adult female Sprague-Dawley rats were subjected to SCI by 30 g clip compression at the level of Th6 and Th7 and were administered BMSC cell sheet (7 × 104 cells, subdural), cell suspension (7 × 104 cells, intramedullary), or control seven days after the injury. Motor and sensory assessments, as well as histological evaluation, were performed to determine the efficacy of the different cell transplantation procedures. While both the cell sheet and cell intramedullary injection groups showed significant motor recovery compared to the control group, the cell sheet group showed better results. Furthermore, the cell sheet group displayed a significant sensory recovery compared to the other groups. A histological evaluation revealed that the cell sheet group showed smaller injury lesion volume, less inflammation, and gliosis compared to other groups. Sensory-related fibers of μ-opioid receptors (MOR, interneuron) and hydroxytryptamine transporters (HTT, descending pain inhibitory pathway), located around the dorsal horn of the spinal cord at the caudal side of the SCI, were preserved only in the cell sheet group. Stem cells could also be found inside the peri-injured spinal cord in the cell sheet group. BMSC cell sheets were able to promote functional recovery and palliate neuropathic pain more effectively than intramedullary injections, thus serving as a good treatment option for SCI.
  • Hiroaki Motegi, Shigeru Yamaguchi, Yukitomo Ishi, Michinari Okamoto, Akihiro Iguchi, Yuko Cho, Minako Sugiyama, Atsushi Manabe, Kiyohiro Houkin
    NEURO-ONCOLOGY 22 329 - 330 2020年12月 [査読有り]
  • Michinari Okamoto, Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Yukayo Terashita, Shinsuke Hirabayashi, Minako Sugiyama, Yuko Cho, Akihiro Iguchi, Atushi Manabe, Kiyohiro Houkin
    NEURO-ONCOLOGY 22 334 - 334 2020年12月 [査読有り]
  • Shigeru Yamaguchi, Hiroaki Motegi, Yukitomo Ishi, Michinari Okamoto, Akihiro Iguchi, Yuko Cho, Minako Sugiyama, Atushi Manabe, Kiyohiro Houkin
    NEURO-ONCOLOGY 22 461 - 461 2020年12月 [査読有り]
  • 脳梗塞に対する新規治療の開発-血栓回収療法時代のアンメットメディカルニーズと医薬品開発戦略- 主幹動脈閉塞脳梗塞患者に対する幹細胞脳内移植治験(RAINBOW研究)
    川堀 真人, 七戸 秀夫, 黒田 敏, 宝金 清博
    脳循環代謝 32 1 52 - 52 (一社)日本脳循環代謝学会 2020年11月
  • 脳梗塞急性期患者への自家BMSC脳内投与による再生治療(Phase 1)とPhase 2に向けた取り組み
    川堀 真人, 七戸 秀夫, 黒田 敏, 宝金 清博
    脳循環代謝 32 1 60 - 60 (一社)日本脳循環代謝学会 2020年11月
  • 脳神経細胞治療・遺伝子治療の実践 脳出血慢性期モデルに対する骨髄幹細胞+足場材製剤(CellSaic)の有効性
    高宮 宗一朗, 川堀 真人, 七戸 秀夫, 中村 健太郎, 北橋 宗, 岩澤 玲子, 古川 友子, 宝金 清博
    脳循環代謝 32 1 68 - 68 (一社)日本脳循環代謝学会 2020年11月
  • ラット亜急性期脊髄損傷モデルに対する骨髄間葉系幹細胞シート移植の治療効果検証
    山崎 和義, 川堀 真人, 関 俊隆, 高宮 宗一朗, 今野 幸太郎, 渡辺 雅彦, 宝金 清博
    脳循環代謝 32 1 82 - 82 (一社)日本脳循環代謝学会 2020年11月
  • Yasuhiro Ito, Takeo Abumiya, Teruyuki Komatsu, Ryosuke Funaki, Masayuki Gekka, Kota Kurisu, Taku Sugiyama, Masahito Kawabori, Toshiya Osanai, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
    Brain research 1746 147012 - 147012 2020年11月01日 [査読有り][通常論文]
     
    Regional cold perfusion and hemoglobin-based oxygen carrier administration both exert neuroprotective effects against cerebral ischemia reperfusion injury. We herein investigated whether the combination of these two therapies leads to stronger neuroprotective effects. Combination therapy was performed with the regional perfusion of cold HemoAct, a core-shell structured hemoglobin-albumin cluster, in a rat transient middle cerebral artery occlusion model. The effects of combination therapy, the intra-arterial administration of 10 °C HemoAct (10H) initiated at the onset of reperfusion, were compared with those of monotherapies, the intra-arterial administration of 10 °C saline (10S) and 37 °C HemoAct (37H), and an untreated control under the condition of 2-hour ischemia/24-hour reperfusion. The durability of therapeutic effects and the therapeutic time window of combination therapy were assessed based on comparisons with the 10H and control groups. Significantly better neurological findings and smaller infarct volumes were observed in the three treated (10S, 37H, and 10H) groups than in the control group. Among the 3 treated groups, only the 10H group showed significant improvements over the control group in the other items examined, including cerebral blood flow reduction, brain edema, and protein extravasation. The significant therapeutic effects of combination therapy on neurological disabilities and infarct volumes were confirmed at least until 7 days after reperfusion. Furthermore, combination therapy ameliorated neurological disabilities and hemorrhagic transformation in rats subjected to 4- and 5-hour ischemia/24-hour reperfusion. Since therapeutic effects may be expected until at least 5 h of complete ischemia and reperfusion, this combination therapy is a promising neuroprotective strategy against severe ischemic stroke.
  • Masahito Kawabori, Hideo Shichinohe, Satoshi Kuroda, Kiyohiro Houkin
    International journal of molecular sciences 21 19 1 - 21 2020年10月06日 [査読有り][通常論文]
     
    Despite recent developments in innovative treatment strategies, stroke remains one of the leading causes of death and disability worldwide. Stem cell therapy is currently attracting much attention due to its potential for exerting significant therapeutic effects on stroke patients. Various types of cells, including bone marrow mononuclear cells, bone marrow/adipose-derived stem/stromal cells, umbilical cord blood cells, neural stem cells, and olfactory ensheathing cells have enhanced neurological outcomes in animal stroke models. These stem cells have also been tested via clinical trials involving stroke patients. In this article, the authors review potential molecular mechanisms underlying neural recovery associated with stem cell treatment, as well as recent advances in stem cell therapy, with particular reference to clinical trials and future prospects for such therapy in treating stroke.
  • Zifeng Wang, Kei Higashikawa, Hironobu Yasui, Yuji Kuge, Yusuke Ohno, Akio Kihara, Yenari A Midori, Kiyohiro Houkin, Masahito Kawabori
    Translational stroke research 11 5 1103 - 1116 2020年10月 [査読有り][通常論文]
     
    Injury due to brain ischemia followed by reperfusion (I/R) may be an important therapeutic target in the era of thrombectomy. FTY720, a widely known sphingosine-1-phosphate receptor agonist, exerts various neuroprotective effects. The aim of this study was to examine the protective effect of FTY720 with respect to I/R injury, especially focusing on blood-brain barrier (BBB) protection and anti-inflammatory effects. Male rats were subjected to transient ischemia and administered vehicle or 0.5 or 1.5 mg/kg of FTY720 immediately before reperfusion. Positron emission tomography (PET) with [18F]DPA-714 was performed 2 and 9 days after the insult to serially monitor neuroinflammation. Bovine and rat brain microvascular endothelial cells (MVECs) were also subjected to oxygen-glucose deprivation (OGD) and reperfusion, and administered FTY720, phosphorylated-FTY720 (FTY720-P), or their inhibitor. FTY720 dose-dependently reduced cell death, the infarct size, cell death including apoptosis, and inflammation. It also ameliorated BBB disruption and neurological deficits compared to in the vehicle group. PET indicated that FTY720 significantly inhibited the worsening of inflammation in later stages. FTY720-P significantly prevented the intracellular redistribution of tight junction proteins but did not increase their mRNA expression. These results suggest that FTY720 can ameliorate I/R injury by protecting the BBB and regulating neuroinflammation.
  • Kikutaro Tokairin, Shuji Hamauchi, Masaki Ito, Ken Kazumata, Taku Sugiyama, Naoki Nakayama, Masahito Kawabori, Toshiya Osanai, Kiyohiro Houkin
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 29 12 105305 - 105305 2020年09月23日 [査読有り][通常論文]
     
    BACKGROUND: Moyamoya disease (MMD) is an occlusive cerebrovascular disease, causing stroke in children and young adults with unknown etiology. The fundamental pathology is fibrocellular intimal thickening of cerebral arteries, in which vascular smooth muscle cells (VSMCs) are observed as one of the major cell types. Although the characteristics of circulating smooth muscle progenitor cells have been previously reported, the VSMCs are poorly characterized in MMD. We aimed to characterize VSMCs in MMD using induced pluripotent stem cell (iPSC)-technology. METHODS: We differentiated VSMCs from neural crest stem cells (NCSCs) using peripheral blood mononuclear cell-derived iPSCs and compared biological and transcriptome features under naïve culture conditions between three independent healthy control (HC) subjects and three MMD patients. VSMC transcriptome profiles were also compared to those of endothelial cells (ECs) differentiated from the same iPSCs. RESULTS: Homogeneous spindle-shaped cells differentiated from iPSCs exhibited smooth muscle cell marker expressions, including α-smooth muscle actin (αSMA, 82.3 ± 6.7% and 81.0 ± 6.7%); calponin (91.3 ± 2.1% and 90.9 ± 1.3%); myosin heavy chain-11 (MYH11, 96.9 ± 0.7% and 97.1 ± 0.3%) without significance of differences between the two groups. Real-time PCR showed few PECAM1 and CD34 gene expressions in both groups, indicating features of differentiated VSMCs. There were no significant differences in cellular proliferation (p = 0.45), migration (p = 0.60), and contractile abilities (p = 0.96) between the two groups. Transcriptome analysis demonstrated similar gene expression profiles of VSMCs in HC subjects and MMD patients with six differentially expressed genes (DEGs); while ECs showed a distinct transcriptome profile in MMD patients with 120 DEGs. The Wnt-signaling pathway was a significant pathway in VSMCs. CONCLUSIONS: This is the first study that established VSMCs from NCSCs using MMD patient-derived iPSCs and demonstrated similar biological function and transcriptome profile of iPSC-derived VMSCs in MMD patients and HC subjects under naïve single culture condition. Comparative transcriptome features between iPSC-derived VSMCs and ECs, displaying distinct transcriptome in the ECs, suggested that pathological traits can be driven by naïve ECs predominantly and VSMCs may require specific environmental factors in MMD, which provides novel insight into the pathophysiology of MMD. Our iPSC derived VSMC model can contribute to further investigations of diagnostic and therapeutic target of MMD in addition to the current iPSC derived EC model.
  • Hisayasu Saito, Daina Kashiwazaki, Haruto Uchino, Shusuke Yamamoto, Kiyohiro Houkin, Satoshi Kuroda
    Acta neurochirurgica 163 2 583 - 592 2020年09月14日 [査読有り][通常論文]
     
    BACKGROUND: There are no reports describing the surgical procedure for moyamoya disease (MMD) patients with severe cerebral ischemia in the territory of the posterior cerebral artery (PCA) at initial presentation. In this study, therefore, we describe their clinical and radiological features and clinical results of one-stage revascularization surgery for both anterior and posterior circulation. METHODS: This study included 6 MMD patients who had severe cerebral ischemia in the PCA territory and underwent one-stage revascularization surgery for both anterior and posterior circulation. Of these, one patient underwent it on both sides. Their clinical and radiological data were precisely analyzed. Compared with usual procedure, craniotomy was extended towards the temporo-parietal area more widely. The parietal branch of superficial temporal artery (STA) was anastomosed to the angular or posterior temporal artery, while the frontal branch was anastomosed to the frontal branch of MCA. Ultimate indirect bypass was added. Their clinical and radiological outcomes were evaluated. RESULTS: Their neurological symptoms included visual and speech disturbance as well as numbness of the extremities. Cerebral infarct was distributed in the posterior temporal, parietal, and/or occipital lobe. Cerebral hemodynamics and metabolism were also impaired in the same regions. These findings were completely different from those in MMD patients without PCA lesion. Postoperative course was uneventful, and none of them recurred stroke during a mean follow-up period of 10.5 years. Surgical collaterals widely provided blood flow to the entire hemispheres, including the occipital lobe. Cerebral hemodynamics and metabolism markedly improved after surgery. CONCLUSION: One-stage revascularization surgery for both anterior and posterior circulation is feasible and effective to prevent future stroke in MMD patients with severe cerebral ischemia in the PCA territory at initial presentation.
  • Michinari Okamoto, Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Takashi Mori, Takayuki Hashimoto, Yukayo Terashita, Shinsuke Hirabayashi, Minako Sugiyama, Akihiro Iguchi, Yuko Cho, Atsushi Manabe, Kiyohiro Houkin
    Oncology 99 1 1 - 9 2020年09月09日 [査読有り][通常論文]
     
    OBJECTIVE: Most types of intracranial germ cell tumors (IGCTs) are sensitive to chemoradiation. However, biopsy specimens are usually small and thus cannot be used for obtaining an accurate pathological diagnosis. Recently, the cerebrospinal fluid (CSF) placental alkaline phosphatase (PLAP) value has been considered a new biomarker of IGCTs. The present study aimed to evaluate the discriminatory characteristics of the CSF-PLAP value upon diagnosis and at the time of recurrence in patients with IGCTs. METHODS: Between 2015 and 2019, this study included 37 patients with tumors located in the intraventricular and/or periventricular region. The CSF-PLAP level was assessed before the patients received any treatment. The PLAP level was evaluated during and after first-line chemoradiotherapy in 7 patients with IGCTs. The CSF-PLAP values were compared according to histological diagnosis, and the correlation between these values and radiographical features was assessed. The CSF-PLAP values of 6 patients with IGCTs with suspected recurrence were evaluated based on neuroimaging findings. RESULTS: The CSF-PLAP values were significantly higher in patients with IGCTs than in those with other types of brain tumor (n = 19 vs. 18; median: 359.0 vs. <8.0 pg/mL). The specificity and sensitivity were 88 and 95%, respectively, with a cutoff value of 8.0 pg/mL. In patients with IGCT, the CSF-PLAP value was higher in patients with germinoma than in those with nongerminomatous germ cell tumors (n = 12 vs. 7; median: 415.0 vs. 359.0 pg/mL). Regarding the time course, the CSF-PLAP value decreased to below the detection limit after the reception of first-line chemoradiotherapy in all 7 patients. A significant correlation was observed between the initial CSF-PLAP value and the tumor reduction volume after receiving first-line chemoradiotherapy (p < 0.0003, R2 = 0.6165, logY = 1.202logX - 1.727). Among the patients with suspected IGCT recurrence (n = 6), the CSF-PLAP value was high in patients with recurrence (n = 3; median: 259.0 pg/mL), and that in patients (n = 3) without recurrence was below the lower detection limit. CONCLUSIONS: The CSF-PLAP level is a useful biomarker during the initial diagnosis of IGCTs and at the time of recurrence. It may be associated with the volume of germinomatous components of tumors.
  • Chengbo Tan, Zifeng Wang, Miao Zheng, Songji Zhao, Hideo Shichinohe, Kiyohiro Houkin
    Journal of Nippon Medical School = Nippon Ika Daigaku zasshi 88 3 228 - 237 2020年08月31日 [査読有り][通常論文]
     
    BACKGROUND: Stroke is a leading cause of death and disability worldwide. Recently, brain secondary damage has been hypothesized to be a key aggravating element in an ischemic cascade. However, the interaction between cerebral infarction and immune organs has yet to be fully understood. In this study, we investigated the changes in the rat brain, spleen, thymus, mesenteric lymph node, and liver at 3, 7, and 13 days after transient middle cerebral artery occlusion (tMCAO) by immunohistochemistry. MATERIAL AND METHODS: Rat models of stroke were made by tMCAO. Functional assessment was performed 3 h, and 1, 3, 5, 7, 9, 11, and 13 days after MCAO. Rat organs were harvested for 2,3,5-triphenyltetrazolium chloride staining and Immunohistochemistry. RESULTS: The CD8α+ T cells was found to decrease in the spleen, thymus, mesenteric lymph node, and liver, whereas it increased in the brain. Those of Iba1+ and CD68+ macrophages were decreased in the spleen, thymus, and mesenteric lymph node, whereas they were elevated in the brain and liver. Ki67+ cells showed the same characteristics as macrophages, and increased numbers of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive apoptotic cells were found in the spleen, mesenteric lymph node, liver, and brain. CONCLUSIONS: The present results demonstrated that stroke is a systemic disease, which not only affects the brain, but also induces responses of immune organs. On the basis of these results, a systemic treatment might be a good strategy for clinical stroke care.
  • Kazuyoshi Yamazaki, Masahito Kawabori, Toshitaka Seki, Soichiro Takamiya, Takahiro Tateno, Kotaro Konno, Masahiko Watanabe, Kiyohiro Houkin
    Journal of neurotrauma 37 15 1720 - 1728 2020年08月01日 [査読有り][通常論文]
     
    Neuropathic pain severely impairs rehabilitation and quality of life after spinal cord injury (SCI). The sphingosine-1-phosphate receptor agonist, FTY720, plays an important protective role in neuronal injury. This study aims to examine the effects of FTY720 in a rat acute SCI model, focusing on neuropathic pain. Female rats with SCI induced by 1-min clip compression were administered vehicle or 1.5 mg/kg of FTY720 24 h after the injury. Using the mechanical nociceptive threshold test, we monitored neuropathic pain and performed histological analysis of the pain pathway, including the μ opioid receptor (MOR), hydroxytryptamine transporter (HTT), and calcitonin gene-related peptide (CGRP). Motor score, SCI lesion volume, residual motor axons, inflammatory response, glial scar, and microvascular endothelial dysfunction were also compared between the two groups. FTY720 treatment resulted in significant attenuation of post-traumatic neuropathic pain. It also decreased systemic and local inflammation, thereby reducing the damaged areas and astrogliosis and resulting in motor functional recovery. Whereas there was no difference in the CGRP expression between the two groups, FTY720 significantly preserved the MOR in both the caudal and rostral areas of the spinal dorsal horn. Whereas HTT was preserved in the FTY720 group, it was significantly increased in the rostral side and decreased in the caudal side of the injury in the vehicle group. These results suggest that FTY720 ameliorates post-traumatic allodynia through regulation of neuroinflammation, maintenance of the blood-brain barrier, and inhibition of glial scar formation, thereby preserving the connectivity of the descending inhibitory pathway and reducing neuropathic pain.
  • Michinari Okamoto, Taku Sugiyama, Naoki Nakayama, Satoshi Ushikoshi, Ken Kazumata, Toshiya Osanai, Kikutaro Tokairin, Yusuke Shimoda, Kiyohiro Houkin
    Operative neurosurgery (Hagerstown, Md.) 19 6 691 - 700 2020年07月27日 [査読有り][通常論文]
     
    BACKGROUND: Pial arterial feeders in an intracranial dural arteriovenous fistula (dAVF) are risk factors for both ischemic and hemorrhagic complications during endovascular embolization. Microsurgery in dAVF with pial arterial feeders has rarely been reported. OBJECTIVE: To assess our original experience with microsurgery for dAVF with pial arterial feeders by investigating surgical findings and outcomes. METHODS: In 40 patients with intracranial dAVF who underwent microsurgery, we found 8 patients who had pial arterial feeders. A retrospective review was conducted. RESULTS: The locations of the dAVFs were as follows: tentorium, 2 patients; Galenic system, 1; straight sinus, 1; torcula, 1; transverse sinus, 1; ethmoid, 1; and convexity, 1. Preoperative angiography revealed that the pial arterial feeders originated from the middle cerebral artery in 2 patients, the anterior cerebral artery in 1, the posterior cerebral artery in 2, and the posterior medial choroidal artery in 1. Of note, in 2 patients (6.7%), feeders from the superior cerebellar artery were determined to be angiographically occult during preoperative examination and were detected through careful intraoperative observation and arachnoid dissection. In 5 cases, the additional obliteration of the pial arterial feeders and/or more distal cortical venous drainers after the simple disconnection of proximal cortical drainers was necessary to cure the dAVF. Finally, all shunts were cured with only 1 minor complication. CONCLUSION: Although microsurgical results were acceptable, the surgeon should be aware of the presence of pial arterial feeders even after the simple disconnection of cortical venous drainage. Angiographically occult feeders may also exist.
  • Kazuyoshi Yamazaki, Masahito Kawabori, Toshitaka Seki, Kiyohiro Houkin
    International journal of molecular sciences 21 11 2020年06月02日 [査読有り][通常論文]
     
    There are more than one million patients worldwide suffering paralysis caused by spinal cord injury (SCI). SCI causes severe socioeconomic problems not only to the patients and their caregivers but also to society; therefore, the development of innovative treatments is crucial. Many pharmacological therapies have been attempted in an effort to reduce SCI-related damage; however, no single therapy that could dramatically improve the serious long-term sequelae of SCI has emerged. Stem cell transplantation therapy, which can ameliorate damage or regenerate neurological networks, has been proposed as a promising candidate for SCI treatment, and many basic and clinical experiments using stem cells for SCI treatment have been launched, with promising results. However, the cell transplantation methods, including cell type, dose, transplantation route, and transplantation timing, vary widely between trials, and there is no consensus regarding the most effective treatment strategy. This study reviews the current knowledge on this issue, with a special focus on the clinical trials that have used stem cells for treating SCI, and highlights the problems that remain to be solved before the widespread clinical use of stem cells can be adopted.
  • Koji Iihara, Teiji Tominaga, Nobuhito Saito, Michiyasu Suzuki, Isao Date, Yukihiko Fujii, Kazuhiro Hongo, Kiyohiro Houkin, Amami Kato, Yoko Kato, Takakazu Kawamata, Phyo Kim, Hiroyuki Kinouchi, Eiji Kohmura, Kaoru Kurisu, Keisuke Maruyama, Nobuhiro Mikuni, Susumu Miyamoto, Akio Morita, Hiroyuki Nakase, Yoshitaka Narita, Ryo Nishikawa, Kazuhiko Nozaki, Kuniaki Ogasawara, Kenji Ohata, Nobuyuki Sakai, Hiroaki Sakamoto, Yoshiaki Shiokawa, Yukihiko Sonoda, Jun C Takahashi, Keisuke Ueki, Toshihiko Wakabayashi, Takamitsu Yamamoto, Kazunari Yoshida, Takamasa Kayama, Hajime Arai
    Neurologia medico-chirurgica 60 4 165 - 190 2020年04月15日 [査読有り][通常論文]
     
    The Japan Neurosurgical Database (JND) is a prospective observational study registry established in 2017 by the Japan Neurosurgical Society (JNS) to visualize real-world clinical practice, promote science, and improve the quality of care and neurosurgery board certification in Japan. We summarize JND's aims and methods, and describes the 2018 survey results. The JND registered in-hospital patients' clinical data mainly from JNS training institutions in 2018. Caseload, patient demographics, and in-hospital outcomes of the overall cohort and a neurosurgical subgroup were examined according to major classifications of main diagnosis. Neurosurgical caseload per neurosurgeon in training in core hospitals in 2018 was calculated as an indicator of neurosurgical training. Of 523,283 cases (male 55.3%) registered from 1360 participating institutions, the neurosurgical subgroup comprised of 33.9%. Among the major classifications, cerebrovascular diseases comprised the largest proportion overall and in the neurosurgical subgroup (53.1%, 41.0%, respectively), followed by neurotrauma (19.1%, 25.5%), and brain tumor (10.4%, 12.8%). Functional neurosurgery (6.4%, 3.7%), spinal and peripheral nerve disorders (5.1%, 10.1%), hydrocephalus/developmental anomalies (2.9%, 5.3%), and encephalitis/infection/inflammatory and miscellaneous diseases (2.9%, 1.6%) comprised smaller proportions. Most patients were aged 70-79 years in the overall cohort and neurosurgical subgroup (27.8%, 29.4%). Neurotrauma and cerebrovascular diseases in the neurosurgical subgroup comprised a higher and lower proportion, respectively, than in the overall cohort in elderly patients (e.g. 80 years, 46.9% vs. 33.5%, 26.8% vs. 54.4%). The 2018 median neurosurgical caseload per neurosurgeon in training was 80.7 (25-75th percentile 51.5-117.5). These initial results from 2018 reveal unique aspects of neurosurgical practice in Japan.
  • Ken Kazumata, Kikutaro Tokairin, Masaki Ito, Haruto Uchino, Taku Sugiyama, Masahito Kawabori, Toshiya Osanai, Khin Khin Tha, Kiyohiro Houkin
    Journal of neurosurgery 134 4 1 - 10 2020年04月03日 [査読有り][通常論文]
     
    OBJECTIVE: The microstructural integrity of gray and white matter is decreased in adult moyamoya disease, suggesting covert ischemic injury as a mechanism of cognitive dysfunction. Establishing a microstructural brain imaging marker is critical for monitoring cognitive outcomes following surgical interventions. The authors of the present study determined the pathophysiological basis of altered microstructural brain injury in relation to advanced arterial occlusion, cerebral hypoperfusion, and cognitive function. METHODS: The authors examined 58 patients without apparent brain lesions and 30 healthy controls by using structural MRI, as well as diffusion tensor imaging (DTI). Arterial occlusion in each hemisphere was classified as early or advanced stage based on MRA and posterior cerebral artery (PCA) involvement. Regional cerebral blood flow (rCBF) was measured with N-isopropyl-p-[123I]-iodoamphetamine SPECT. Furthermore, cognitive performance was examined using the Wechsler Adult Intelligence Scale, Third Edition and the Trail Making Test (TMT). Both voxel- and region of interest-based analyses were performed for groupwise comparisons, as well as correlation analysis, using parameters such as cognitive test scores; gray matter volume; fractional anisotropy (FA) of association fiber tracts, including the inferior frontooccipital fasciculus (IFOF) and superior longitudinal fasciculus (SLF); PCA involvement; and rCBF. RESULTS: Compared to the early stages, advanced stages of arterial occlusion in the left hemisphere were associated with a lower Performance IQ (p = 0.031), decreased anterior cingulate volumes (p = 0.0001, uncorrected), and lower FA in the IFOF, cingulum, and forceps major (all p < 0.01, all uncorrected). There was no significant difference in rCBF between the early and the advanced stage. In patients with an advanced stage, PCA involvement was correlated with a significantly lower Full Scale IQ (p = 0.036), cingulate volume (p < 0.01, uncorrected), and FA of the left SLF (p = 0.0002, uncorrected) compared to those with an intact PCA. The rCBF was positively correlated with FA of the SLF, IFOF, and forceps major (r > 0.34, p < 0.05). Global gray matter volumes were moderately correlated with TMT part A (r = 0.40, p = 0.003). FA values in the left SLF were moderately associated with processing speed (r = 0.40, p = 0.002). CONCLUSIONS: Although hemodynamic compensation may mask cerebral ischemia in advanced stages of adult moyamoya disease, the disease progression is detrimental to gray and white matter microstructure as well as cognition. In particular, additional PCA involvement in advanced disease stages may impair key neural substrates such as the cingulum and SLF. Thus, combined structural MRI and DTI are potentially useful for tracking the neural integrity of key neural substrates associated with cognitive function and detecting subtle anatomical changes associated with persistent ischemia, as well as disease progression.
  • 後藤秀輔, 長内俊也, 牛越聡, 栗栖宏多, 下田祐介, 寳金清博
    Neurological Surgery 48 4 335 - 340 2020年04月 [査読有り][通常論文]
     
    BACKGROUND: Le Fort I osteotomy, one of the most common techniques applied to correct jaw deformities, is generally considered operatively safe. However, a few articles reported that this procedure can lead to formation of arteriovenous fistulae(AVF)involving the maxillary artery infrequently. CASE REPORT: A man in his 40s with a jaw deformity underwent Le Fort I osteotomy. Two days later, he noticed pulsating tinnitus in his right ear. Angiography revealed AVF between the proximal part of the maxillary artery(first segment)and the pterygoid plexus. On further evaluation, AVF were embolized with n-butyl-2-cyanoacrylate(NBCA). Obliteration of AVF was confirmed on the final angiography. The tinnitus resolved shortly after the embolization. CONCLUSION: Embolization with NBCA is a promising treatment for maxillary AVF after Le Fort I osteotomy.
  • Takeshi Mikami, Hime Suzuki, Ryo Ukai, Katsuya Komatsu, Yukinori Akiyama, Masahiko Wanibuchi, Kiyohiro Houkin, Nobuhiro Mikuni
    Neurosurgical review 43 2 609 - 617 2020年04月 [査読有り][通常論文]
     
    Extracranial-to-intracranial (EC-IC) bypass surgery is an effective treatment for patients with moyamoya disease and other conditions. Some patients with moyamoya disease have a risk of acute thrombogenesis at the anastomotic site just after bypass surgery. The purpose of this study was to study risk factors of acute thrombogenesis and determine effective countermeasures. This study included 48 patients (66 EC-IC bypass procedures) with moyamoya disease and 52 controls (54 procedures) without moyamoya disease. The development of acute thrombogenesis was compared between the moyamoya disease and control groups. In the moyamoya disease group, clinical and radiological characteristics were assessed with respect to acute thrombogenesis. In the patients with acute thrombogenesis, causes of technical problems were retrospectively examined. The incidence of acute thrombogenesis was significantly higher in the moyamoya disease group than those in the control group. In the moyamoya disease group, acute thrombogenesis was observed in seven patients. In the moyamoya disease group, the magnetic resonance angiography (MRA) scores were significantly higher in patients with acute thrombogenesis than those in the patients without acute thrombogenesis. In the multivariate analysis, the predictive factor of acute thrombogenesis in moyamoya disease was a high MRA score (odds ratio, 2.336; p = 0.009). During EC-IC bypass surgery for moyamoya disease, acute thrombogenesis should be considered to obtain a high patency rate, particularly in patients with high MRA scores. Acute thrombogenesis will not influence morbidity if proper countermeasures are followed; therefore, the prediction and recognition of white thrombus are important for a successful bypass surgery.
  • Fusao Ikawa, Akio Morita, Takeo Nakayama, Yoshihito Goto, Nobuyuki Sakai, Koji Iihara, Yoshiaki Shiokawa, Isao Date, Kazuhiko Nozaki, Hiroyuki Kinouchi, Kiyohiro Houkin, Nobuhito Saito, Teiji Tominaga, Michiyasu Suzuki, Susumu Miyamoto, Kaoru Kurisu, Hajime Arai
    Journal of neurosurgery 134 3 1 - 9 2020年03月27日 [査読有り][通常論文]
     
    OBJECTIVE: Japan has been reported to have the highest (and increasing) incidence of subarachnoid hemorrhage (SAH) in the world. However, there has never been a report on the nationwide incidence rate and recent trends for SAH in Japan. In this register-based study, the authors aimed to clarify the estimated nationwide SAH incidence rate and the recent trend in SAH incidence in Japan and the reasons for any changes in this trend. METHODS: The authors compiled data from the Japanese Ministry of Health, Labour and Welfare and from the records of the Japan Neurosurgical Society. They reviewed the age-standardized nationwide SAH mortality rate, the estimated age-standardized SAH incidence rate according to the age-standardized SAH mortality rate, and the estimated crude SAH incidence rate, including the 95% confidence intervals, from 2003 to 2015. The trends in the number of treatments for unruptured and ruptured cerebral aneurysms, as well as the prevalence of hypertension, current smoking status, and use of cholesterol-lowering drugs, were assessed. The estimated treatment rate for unruptured cerebral aneurysms (UCAs) was also calculated along with the 95% confidence interval. RESULTS: The estimated age-standardized nationwide SAH incidence rate significantly declined from 31.34 cases (95% CI 31.32-31.34) to 27.63 (95% CI 27.59-27.63; decrease by 11.8%) per 100,000 person-years. This decline was based on the 2010 population as the reference from 2003 through 2015 and a case-fatality rate of SAH that was assumed to decrease by 1% annually from 44% in 2003 to 32% in 2015. According to sensitivity analysis, the change rate of the estimated age-standardized SAH incidence rate ranged from -56.69% to 23.27%, with a mean of -30.91% (SD 20.52%), and there were decline trends in 92% of all trends. The estimated nationwide crude SAH incidence rate also showed a significant decline from 23.79 (95% CI 23.78-23.79) to 20.25 (95% CI 20.24-20.25; decrease by 14.9%) per 100,000 person-years. The trend in treatment numbers for UCAs increased significantly (p < 0.0001) from 2003 through 2015; however, the estimated treatment rate for UCAs was only 0.19 (95% CI 0.19-0.20) to 0.51 (95% CI 0.50-0.51) among all UCA patients. The prevalence of hypertension (males, p = 0.0003; females, p < 0.0001) and current smoking status (males, p < 0.0001; females, p = 0.0002) declined significantly from 2003 through 2015, while the use of cholesterol-lowering drugs increased significantly (males, p < 0.0001; females, p = 0.0005) during the same period. CONCLUSIONS: The estimated nationwide SAH incidence rate in Japan was higher than rates in other countries, although it has declined recently. An improving lifestyle may have contributed to the declining rate of SAH incidence in Japan.
  • Satoshi Kuroda, Naoki Nakayama, Shusuke Yamamoto, Daina Kashiwazaki, Haruto Uchino, Hisayasu Saito, Emiko Hori, Naoki Akioka, Naoya Kuwayama, Kiyohiro Houkin
    Journal of neurosurgery 134 3 1 - 8 2020年03月13日 [査読有り][通常論文]
     
    OBJECTIVE: Surgical revascularization is known to reduce the incidence of further ischemic and hemorrhagic events in patients with moyamoya disease, but the majority of previous studies report only short-term (< 5 years) outcomes. Therefore, in this study the authors aimed to evaluate late (5-20 years) outcomes of moyamoya patients after superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis and indirect bypass (encephalo-duro-myo-arterio-pericranial synangiosis [EDMAPS]). METHODS: Cumulative incidences of late morbidity/mortality and disease progression were evaluated among 93 patients who underwent STA-MCA anastomosis and EDMAPS. All of the patients were prospectively followed up for longer than 5 years postsurgery (10.5 ± 4.4 years). There were 35 pediatric and 58 adult patients. Initial presentation included transient ischemic attack/ischemic stroke in 80 patients and hemorrhagic stroke in 10 patients, and 3 patients were asymptomatic. Surgery was performed in a total of 141 hemispheres. Follow-up MRI/MRA was performed within a 6- or 12-month interval during the follow-up periods. RESULTS: During the follow-up periods, 92/93 patients were free from any stroke or death, but 1 patient had a recurrence of hemorrhagic stroke (0.10% per patient-year). Disease progression occurred in the territory of the contralateral carotid or posterior cerebral artery (PCA) in 19 hemispheres of 15 patients (1.5% per patient-year). The interval between initial surgery and disease progression varied widely, from 0.5 to 15 years. Repeat bypass surgery for the anterior and posterior circulation resolved ischemic attacks in all 10 patients. CONCLUSIONS: The study results indicate that STA-MCA anastomosis and EDMAPS would be the best choice to prevent further ischemic and hemorrhagic stroke for longer than 10 years on the basis of the demonstrated widespread improvement in cerebral hemodynamics in both the MCA and ACA territories in the study patients. However, after 10 years postsurgery regular follow-up is essential to detect disease progression in the territory of the contralateral carotid artery and PCA and prevent late cerebrovascular events.
  • Jun C Takahashi, Takeshi Funaki, Kiyohiro Houkin, Satoshi Kuroda, Miki Fujimura, Yasutake Tomata, Susumu Miyamoto
    Journal of neurosurgery 134 3 1 - 6 2020年03月13日 [査読有り][通常論文]
     
    OBJECTIVE: Here, the authors aimed to determine whether the presence of cerebral hemodynamic failure predicts subsequent bleeding attacks and how it correlates with the effect of direct bypass surgery in hemorrhagic moyamoya disease. METHODS: Data from the Japanese Adult Moyamoya (JAM) Trial were used in this study: 158 hemispheres in 79 patients. A newly formed expert panel evaluated the SPECT results submitted at trial enrollment and classified the cortical hemodynamic state of the middle cerebral artery territory of each hemisphere into one of the following three groups: SPECT stage (SS) 0 as normal, SS1 as decreased cerebrovascular reserve (CVR), and SS2 as decreased CVR with decreased baseline blood flow. In the nonsurgical cohort of the JAM Trial, the subsequent hemorrhage rate during the 5-year follow-up was compared between the SS0 (hemodynamic failure negative) and SS1+2 (hemodynamic failure positive) groups. The effect of direct or combined direct/indirect bypass surgery on hemorrhage prevention was examined in each subgroup. RESULTS: The hemodynamic grade was SS0 in 59 (37.3%) hemispheres, SS1 in 87 (55.1%), and SS2 in 12 (7.6%). In the nonsurgical cohort, subsequent hemorrhage rates in the SS0 and SS1+2 groups were 12 cases per 1000 person-years and 67 cases per 1000 person-years, respectively. Kaplan-Meier analysis revealed that hemorrhagic events were significantly more common in the SS1+2 group (p = 0.019, log-rank test). Cox regression analysis showed that hemodynamic failure was an independent risk factor for subsequent hemorrhage (HR 5.37, 95% CI 1.07-27.02). In the SS1+2 subgroup, bypass surgery significantly suppressed hemorrhagic events during 5 years (p = 0.001, HR 0.16, 95% CI 0.04-0.57), with no significant effect in the SS0 group (p = 0.655, HR 1.56, 95% CI 0.22-11.10). Examination of effect modification revealed that the effect of surgery tended to differ nonsignificantly between these two subgroups (p = 0.056). CONCLUSIONS: Hemodynamic failure is an independent risk factor for subsequent hemorrhage in hemorrhagic moyamoya disease. Direct bypass surgery showed a significant preventive effect in the hemodynamically impaired hemispheres. Thus, hemodynamic failure, as well as previously proposed factors such as choroidal anastomosis, should be considered for the surgical indication in hemorrhagic moyamoya disease.Clinical trial registration no.: C000000166 (umin.ac.jp).
  • Eguchi K, Yabe I, Shirai S, Iwata I, Matsushima M, Yamazaki K, Hamauchi S, Sasamori T, Seki T, Houkin K, Sasaki H
    Interdisciplinary Neurosurgery 19 100565 - 100565 2020年03月 [査読有り][通常論文]
  • Smile Echizenya, Yukiko Ishii, Satoshi Kitazawa, Tadashi Tanaka, Shun Matsuda, Eriko Watanabe, Masao Umekawa, Shunsuke Terasaka, Kiyohiro Houkin, Tomohisa Hatta, Tohru Natsume, Yoshimasa Maeda, Shin-Ichi Watanabe, Shinji Hagiwara, Toru Kondo
    Neuro-oncology 22 2 229 - 239 2020年02月20日 [査読有り][通常論文]
     
    BACKGROUND: Glioblastoma-initiating cells (GICs) comprise a tumorigenic subpopulation of cells that are resistant to radio- and chemotherapies and are responsible for cancer recurrence. The aim of this study was to identify novel compounds that specifically eradicate GICs using a high throughput drug screening approach. METHODS: We performed a cell proliferation/death-based drug screening using 10 560 independent compounds. We identified dihydroorotate dehydrogenase (DHODH) as a target protein of hit compound 10580 using ligand-fishing and mass spectrometry analysis. The medical efficacy of 10580 was investigated by in vitro cell proliferation/death and differentiation and in vivo tumorigenic assays. RESULTS: Among the effective compounds, we identified 10580, which induced cell cycle arrest, decreased the expression of stem cell factors in GICs, and prevented tumorigenesis upon oral administration without any visible side effects. Mechanistic studies revealed that 10580 decreased pyrimidine nucleotide levels and enhanced sex determining region Y-box 2 nuclear export by antagonizing the enzyme activity of DHODH, an essential enzyme for the de novo pyrimidine synthesis. CONCLUSION: In this study, we identified 10580 as a promising new drug against GICs. Given that normal tissue cells, in particular brain cells, tend to use the alternative salvage pathway for pyrimidine synthesis, our findings suggest that 10580 can be used for glioblastoma therapy without side effects.Key Points1.  Chemical screening identified 10580 as a novel GIC-eliminating drug that targets DHODH, an essential enzyme for the de novo pyrimidine synthesis pathway. 2. Compound 10580 induced cell cycle arrest, apoptosis, and differentiation in GICs.
  • Haruto Uchino, Ken Kazumata, Masaki Ito, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
    Neurosurgical review 44 1 599 - 605 2020年02月19日 [査読有り][通常論文]
     
    Cerebral hyperperfusion (HP) complicates the postoperative course of patients with moyamoya disease (MMD) after direct revascularization surgery. Crossed cerebellar diaschisis (CCD) has been considered to be rarely associated with HP after revascularization surgery. This study aimed to describe the clinical features and factors associated with CCD secondary to cerebral HP after revascularization surgery for MMD. We analyzed 150 consecutive hemispheres including 101 in adults and 49 in pediatric patients who underwent combined direct and indirect bypass for MMD. Using single-photon emission computed tomography (SPECT), serial cerebral blood flow (CBF) was measured immediately after the surgery and on postoperative days 2 and 7. Pre- and postoperative voxel-based analysis of SPECT findings was performed to compare the changes in regional CBF. Multivariate logistic regression analysis was performed to test the effect of multiple variables on CCD. Asymptomatic and symptomatic HP was observed in 41.3% (62/150) and 16.7% (25/150) of the operated hemispheres, respectively. CCD was observed in 18.4% (16/87) of these hemispheres with radiological HP. Multivariate analysis revealed that the occurrence of CCD was significantly associated with symptomatic HP (p = 0.0015). Voxel-based analysis showed that the CBF increase in the operated frontal cortex, and the CBF reduction in the contralateral cerebellar hemisphere on day 7 were significantly larger in symptomatic HP than in asymptomatic HP (median 11.3% vs 7.5%; - 6.0% vs - 1.7%, respectively). CCD secondary to postoperative HP is more common than anticipated in MMD. CCD could potentially be used as an indicator of severe postoperative HP in patients with MMD.
  • Yukitomo Ishi, Shigeru Yamaguchi, Kanako C Hatanaka, Michinari Okamoto, Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
    Journal of neurosurgery 134 3 1 - 9 2020年02月14日 [査読有り][通常論文]
     
    OBJECTIVE: The authors aimed to investigate genetic alterations in low-grade gliomas (LGGs) in pediatric and young adult patients presenting with spontaneous hemorrhage. METHODS: Patients younger than 30 years of age with a pathological diagnosis of World Health Organization (WHO) grade I or II glioma and who had undergone treatment at the authors' institution were retrospectively examined. BRAF V600E, FGFR1 N546/K656, IDH1 R132, IDH2 R172, and KIAA1549-BRAF (K-B) fusion genetic alterations were identified, and the presence of spontaneous tumoral hemorrhage was recorded. RESULTS: Among 66 patients (39 with WHO grade I and 27 with grade II tumors), genetic analysis revealed K-B fusion in 18 (27.3%), BRAF V600E mutation in 14 (21.2%), IDH1/2 mutation in 8 (12.1%), and FGFR1 mutation in 4 (6.1%). Spontaneous hemorrhage was observed in 5 patients (7.6%); 4 of them had an FGFR1 mutation and 1 had K-B fusion. Univariate analysis revealed a statistically significant association of an FGFR1 mutation and a diencephalic location with spontaneous hemorrhage. Among 19 diencephalic cases including the optic pathway, hypothalamus, and thalamus, an FGFR1 mutation was significantly associated with spontaneous hemorrhage (p < 0.001). Four FGFR1 mutation cases illustrated the following results: 1) a 2-year-old female with pilomyxoid astrocytoma (PMA) harboring the FGFR1 K656E mutation presented with intraventricular hemorrhage (IVH); 2) a 6-year-old male with PMA harboring FGFR1 K656E and D652G mutations presented with intratumoral hemorrhage (ITH); 3) a 4-year-old female with diffuse astrocytoma harboring FGFR1 K656M and D652G mutations presented with IVH; and 4) a young adult patient with pilocytic astrocytoma with the FGFR1 N546K mutation presented with delayed ITH and IVH after 7 years of observation. CONCLUSIONS: Although the mechanism remains unclear, the FGFR1 mutation is associated with spontaneous hemorrhage in pediatric and young adult LGG.
  • Yusuke Shimoda, Naoki Nakayama, Takuya Moriwaki, Takeo Abumiya, Masahito Kawabori, Kota Kurisu, Masayuki Gekka, Masaaki Hokari, Yasuhiro Ito, Kiyohiro Houkin
    Journal of neurosurgical sciences 2020年02月04日 [査読有り][通常論文]
     
    BACKGROUND: It is necessary and useful to obtain an experimental model which steadily and rapidly induces aneurysms for investigation of the pathogenesis of cerebral aneurysm. We attempted to examine whether intraperitoneal administration of β-aminopropionitrile fumarate (BAPN-F) with additional treatments of induced hypertension and hemodynamic stress could steadily and rapidly induce aneurysms in male rats. METHODS: Seven-week-old male Sprague-Dawley rats pretreated with ligation of left common carotid and bilateral posterior renal arteries were administrated BAPN-F intraperitoneally. Induction rate and size of aneurysms was investigated with varying dose and duration of BAPN-F administration (low dose; 400 mg/kg/week for 4 or 8 weeks and high dose; 2800 mg/kg/week for 8 or 12 weeks). RESULTS: Induction rate in the high-dose groups was significantly higher (p<0.01) than that in the low-dose groups. Making comparisons between 8 and 12 weeks of the high-dose groups, while there was no difference in induction rate (8 weeks; 85.2% vs 12 weeks; 76.9%), aneurysmal size was larger in 12 weeks (8 weeks; 127.5 μm, vs 12 weeks; 181.7 μm in terms of median) but lethal intrathoracic hemorrhage was increased in 12 weeks (8 weeks; 7.4% vs 12 weeks; 30.8%). Induction rate of large aneurysm was 22.2% and 30.8% in 8 and 12 weeks of the high-dose groups, respectively. CONCLUSIONS: High-dose BAPN-F administration can cause high-frequency aneurysmal induction. Although there was the difference in size and mortality rate based on administration duration, intraperitoneal administration of 2800 mg/kg/week BAPN-F for 8 weeks would be suitable for aneurysmal induction.
  • Ikuma Echizenya, Kikutaro Tokairin, Masahito Kawabori, Ken Kazumata, Kiyohiro Houkin
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 29 2 104549 - 104549 2020年02月 [査読有り][通常論文]
     
    Ring finger protein (RNF) 213 is known as a susceptibility gene for moyamoya disease (MMD), which is characterized by bilateral carotid folk stenosis. Cerebral angiopathy after viral infection has been known to present angiographical appearance resembling MMD, however its pathogenesis and genetic background are not well known. We report a case of reversible cerebral angiopathy after viral infection in a pediatric patient with genetic variant of RNF213 mutation. The patient had developed a severe headache after hand, foot, and mouth disease. Magnetic resonance imaging and magnetic resonance angiography (MRA) performed 2-3 weeks after disease onset revealed bilateral carotid folk stenosis and an old cerebral infarction in the left putamen. The patient's headache spontaneously resolved and the follow-up MRA showed a complete spontaneous resolution of the arterial stenosis after 9 months. We were able to determine genetic predisposition to angiopathy by identifying the RNF213 c.14576G>A (rs112735431, p.R4859K) mutation. Based on the present case, we hypothesize that an RNF213 variant might play an important role for the onset of postviral cerebral angiopathy.
  • Taku Sugiyama, Naoki Nakayama, Satoshi Ushikoshi, Ken Kazumata, Michinari Okamoto, Masaki Ito, Toshiya Osanai, Yusuke Shimoda, Kazuki Uchida, Daisuke Shimbo, Yasuhiro Ito, Katsuyuki Asaoka, Toshitaka Nakamura, Satoshi Kuroda, Kiyohiro Houkin
    Neurosurgical review 44 1 435 - 450 2020年01月02日 [査読有り][通常論文]
     
    Although microsurgery is an established treatment modality for intracranial dural arteriovenous fistula (dAVF), data regarding the perioperative complication rate, cure rate, and long-term outcomes remain scarce. The aims of this study were to describe our original experience with microsurgery, including the surgical complications and pitfalls, and conduct a systematic review of the relevant literature. A multicenter cohort of patients with dAVF treated by microsurgery was retrospectively assessed. In addition, the PubMed database was searched for published studies involving microsurgery for dAVF, and the complication rate, cure rate, and long-term outcomes were estimated. The total number of patients in our multicenter series and published articles was 553 (593 surgeries). The overall rates of transient complications, permanent complications, death, and incomplete treatment were 11.4, 4.0, 1.2, and 6.5%, respectively. A favorable outcome was achieved for 90.1% patients, even though almost half of the patients presented with intracranial hemorrhage. Of note, the incidence of recurrence was only one per 8241 patient-months of postoperative follow-up. Surgeries for anterior cranial fossa dAVF were associated with a lower complication rate, whereas those for tentorial dAVF were associated with higher complication and incomplete treatment rates. The complication and incomplete treatment rates were lower with simple disconnection of cortical venous drainage than with radical occlusion/resection of dural shunts. Our findings suggest that the cure rate, complication rates, and outcomes of microsurgery for dAVF are acceptable; thus, it could be a feasible second-line treatment option for dAVF. However, surgeons should be aware of the specific adverse events of microsurgery.
  • 山崎和義, 川堀真人, 川堀真人, 関俊隆, 高宮宗一朗, 寳金清博, 寳金清博
    日本脳神経外傷学会プログラム・抄録集 43rd 2020年
  • 山口秀, 茂木洋晃, 伊師雪友, 岡本迪成, 澤谷亮佑, 寳金清博
    日本脳腫瘍の外科学会プログラム・抄録集 25th 2020年
  • Sogo Oki, Masahito Kawabori, Sumire Echizenya, Yusuke Shimoda, Daisuke Shimbo, Toshiya Osanai, Kazuki Uchida, Kiyohiro Houkin
    Frontiers in neurology 11 572589 - 572589 2020年 
    Endovascular thrombectomy (EVT) is the preferred treatment strategy for patients with acute ischemic stroke (AIS). However, clinical outcome and prognosis in patients who undergo EVT in response to AIS with concomitant malignancy have not been fully elucidated. Data of patients with malignancy who underwent EVT at participating institutions between January 2015 and April 2019 were retrospectively analyzed. Patient characteristics, treatment methods, posttreatment strategy, and long-term prognosis were evaluated in 12 patients with prediagnoses of malignancy. Good revascularization (TICI 2b or higher) was achieved in 10 of 12 patients. Among the eight patients who survived more than 2 weeks from onset, four patients showed good clinical outcome [modified Rankin Scale (mRS) <2] at 60 days posttreatment and were able to continue treatment for malignancy. However, seven of eight patients died within a year of EVT (median survival, 83 days) due to progression of malignancy. One-year survival was achieved in only one patient whose etiology of stroke was determined as infectious endocarditis and not Trousseau syndrome. Even after successful revascularization and good short-term clinical outcome, the long-term prognosis after thrombectomy in patients with malignancy was poor. Thrombectomy for concomitant malignancy requires judicious decision, and further studies are necessary to fully elucidate its efficacy.
  • Masahito Kawabori, Aki Tanimori, Shinri Kitta, Hideo Shichinohe, Kiyohiro Houkin
    Stem cells international 2020 4085617 - 4085617 2020年 [査読有り][通常論文]
     
    Cell therapy for central nervous system (CNS) disorders is beginning to prove its safety and efficiency. Intraparenchymal transplantation can be an option for cell delivery; however, one concern regarding this method is that the transplantation cannula may cause additional brain injuries. These include vessel damage, which results in brain hemorrhage, and clogging of the cannula by brain debris and/or cell clusters, which requires replacement of the cannula or forced injection causing jet flow of the cell suspension. We compared cannulas for cell delivery used in clinical trials, the Pittsburg and Mizuho cannulas, to a newly designed one, MK01, to assess their usability. MK01 has a spherical-shaped tip with a fan-like open orifice on the side of the cannula, which prevents vessel damage, clogging of brain debris, and jet flow phenomenon. We compared the extent of rat cervical and abdominal arterial damage with the cannula, the amount of debris in the cannula, the force needed to cause jet flow, and cell viability. While the viability of cells passed through the cannulas was almost the same among cannulas (approximately 95%), the Pittsburg cannula caused cervical arterial injury and subsequent hemorrhage, as it required a significantly smaller force to penetrate the arterial wall. Moreover, the Pittsburg cannula, but not the Mizuho and MK01 cannulas, showed high frequency of brain debris in the needle tip (approximately 80%) after brain puncture. While jet flow of the injection liquid was observed even when using smaller forces in the Pittsburg and Mizuho cannulas, MK01 constantly showed low jet flow occurrence. Thus, MK01 seems to be safer than the previously reported cannulas, although further investigation is necessary to validate its safety for clinical use.
  • Zifeng Wang, Masahito Kawabori, Kiyohiro Houkin
    Current medicinal chemistry 27 18 2979 - 2993 2020年 [査読有り][通常論文]
     
    FTY720 (Fingolimod) is a known sphingosine-1-phosphate (S1P) receptor agonist that exerts strong anti-inflammatory effects and was approved as the first oral drug for the treatment of multiple sclerosis by the US Food and Drug Administration (FDA) in 2010. FTY720 is mainly associated with unique functional "antagonist" and "agonist" mechanisms. The functional antagonistic mechanism is mediated by the transient down-regulation and degradation of S1P receptors on lymphocytes, which prevents lymphocytes from entering the blood stream from the lymph node. This subsequently results in the development of lymphopenia and reduces lymphocytic inflammation. Functional agonistic mechanisms are executed through S1P receptors expressed on the surface of various cells including neurons, astrocytes, microglia, and blood vessel endothelial cells. These functions might play important roles in regulating anti-apoptotic systems, modulating brain immune and phagocytic activities, preserving the Blood-Brain-Barrier (BBB), and the proliferation of neural precursor cells. Recently, FTY720 have shown receptor-independent effects, including intracellular target bindings and epigenetic modulations. Many researchers have recognized the positive effects of FTY720 and launched basic and clinical experiments to test the use of this agent against stroke. Although the mechanism of FTY720 has not been fully elucidated, its efficacy against cerebral stroke is becoming clear, not only in animal models, but also in ischemic stroke patients through clinical trials. In this article, we review the data obtained from laboratory findings and preliminary clinical trials using FTY720 for stroke treatment.
  • Shigeru Yamaguchi, Shunsuke Terasaka, Michinari Okamoto, Yukitomo Ishi, Hiroaki Motegi, Hiroyuki Kobayashi, Kiyohiro Houkin
    World neurosurgery 132 e710-e715 - E715 2019年12月 [査読有り][通常論文]
     
    BACKGROUND: Dural reconstruction after transpetrosal approaches is complicated because complete primary closure of presigmoid dura mater is difficult to achieve. To address this problem, we use biocompatible polyglycolic acid (PGA) felt (Durawave) to reconstruct dural defects. To evaluate the use of PGA felt in dural reconstruction, we compared these results with those after conventional duraplasty using autologous fascia grafts. METHODS: We retrospectively surveyed all cases involving a transpetrosal approach reported since 2013. In the conventional procedure, autologous fascia was fixed over the dural defect using stay sutures; any remaining dead space was obliterated by placing abdominal fat grafts. Since April 2017, we have used PGA felt instead of fascia. RESULTS: Of the 37 cases identified, 27 were reconstructed according to the conventional procedure, and the remaining 10 cases were reconstructed using PGA felt. Among the 27 conventional cases, 8 involved cerebrospinal fluid (CSF)-related complications, including 3 cases of rhinorrhea and 5 cases of subcutaneous fluid collection, and 2 cases (7%) required repair surgery. Of the 10 cases involving PGA felt, 1 case (10%) involved subcutaneous fluid collection and required repair surgery, and whereas the remaining 9 cases had no evidence of CSF leakage. In addition, the median dural reconstruction time using PGA felt was 9 minutes, significantly shorter than that when autologous fascia was used (median, 44 minutes). CONCLUSIONS: Using PGA felt for presigmoid dura simplifies dural reconstruction because it obviates the need to suture in a deep field. PGA felt has the potential to prevent CSF-related complications after transpetrosal approaches.
  • Hime Suzuki, Takeshi Mikami, Tomoaki Tamada, Ryo Ukai, Yukinori Akiyama, Akinori Yamamura, Kiyohiro Houkin, Nobuhiro Mikuni
    Neurosurgical review 43 6 1565 - 1573 2019年11月04日 [査読有り][通常論文]
     
    Advances in the understanding of the pathogenesis of arteriosclerosis, abdominal aorta aneurysms and dissections, and carotid artery plaques have focused on chronic inflammation. In this study, we report that inflammatory changes of thrombi contribute to the enlargement and growth of giant intracranial thrombotic aneurysms. Surgical and postmortem samples were collected from 12 cases of large or giant intracranial thrombotic aneurysms diagnosed via pathological investigations. Degeneration of the aneurysmal wall and the infiltration of inflammatory cells in the thrombi were assessed. The number of blood cells and immunohistochemical stain-positive cells was enumerated, and the inflammation and neovascularization in the thrombi were assessed. In all cases, the appearance of inflammatory cells (CD68+ cells, CD206+ cells, lymphocytes, and neutrophils) was apparent in the thrombi. The number of CD34+ cells was moderately correlated with the number of CD68+ cells, and CD34+ cells significantly and strongly correlated with the number of CD206+ cells. Based on the number of neutrophils per CD68+ cells, we classified the cases into 2 groups: a macrophage inflammation-dominant group and a neutrophilic inflammation-dominant group. The neutrophilic inflammation-dominant group had significantly more cases with previous treatments and neurological symptoms due to mass effect than the macrophage inflammation-dominant group. Chronic inflammation due to macrophages in thrombi is a fundamental mechanism in the enlargement of an intracranial thrombotic aneurysm, and neutrophilic inflammation can accelerate this process. Microvascularization in thrombi is linked to inflammation and might promote thickening of the intima and repeated intimal microbleeds.
  • Shusuke Yamamoto, Takeshi Funaki, Miki Fujimura, Jun C Takahashi, Haruto Uchino, Kiyohiro Houkin, Teiji Tominaga, Susumu Miyamoto, Satoshi Kuroda
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 28 11 104328 - 104328 2019年11月 [査読有り][通常論文]
     
    OBJECTIVE: Present study was aimed to precisely evaluate the angio-architectures in patients with asymptomatic moyamoya disease (MMD) by comparing with those with hemorrhagic stroke. METHODS: This study used the data set of cerebral angiography in Asymptomatic Moyamoya Registry (AMORE) Study and Japan Adult Moyamoya (JAM) Trial at enrollment. The development of 3 subtypes of collateral vessels, including lenticulostriate, thalamic, and choroidal anastomosis, was evaluated on cerebral angiography. Suzuki's angiographical stage and posterior cerebral artery (PCA) involvement were also assessed. These findings were compared between asymptomatic (AMORE) and hemorrhagic (JAM) groups. RESULTS: This study included 55 hemispheres of 35 patients in asymptomatic group and 75 hemispheres of 75 patients in hemorrhagic group. In asymptomatic group, thalamic anastomosis was less developed than in hemorrhagic group (P = .011), but there were no significant differences in the development of lenticulostriate and choroidal anastomosis between the 2 groups (P = .077 and P = .26, respectively). Suzuki's stage was more progressed and the prevalence of PCA involvement was significantly higher in hemorrhagic group than in asymptomatic group (P = .0033 and P = .016, respectively). CONCLUSIONS: This study reveals no significant differences in the development of choroidal anastomoses between asymptomatic and hemorrhagic-onset MMD. On the other hand, disease stage and PCA involvement were less advanced in asymptomatic MMD than in hemorrhagic-onset MMD. These findings strongly suggest a certain subgroup of asymptomatic patients with MMD is at potential risk for hemorrhagic stroke.
  • Ken Kazumata, Kikutaro Tokairin, Taku Sugiyama, Masaki Ito, Haruto Uchino, Toshiya Osanai, Masahito Kawabori, Naoki Nakayama, Kiyohiro Houkin
    Journal of neurosurgery. Pediatrics 1 - 7 2019年10月11日 [査読有り][通常論文]
     
    OBJECTIVE: The cognitive effects of main cerebral artery occlusive lesions are unclear in children with moyamoya disease (MMD). The authors aimed to investigate cognitive function in the presurgical phase of pediatric patients with MMD with no apparent brain lesions. METHODS: In this prospective, observational, single-center study, 21 children (mean age 10 ± 3.0 years, range 5-14 years) diagnosed with MMD at Hokkaido University Hospital between 2012 and 2018 were enrolled. A cross-sectional evaluation of intellectual ability was performed using the Wechsler Intelligence Scale for Children-Fourth Edition at the initial diagnosis. rCBF was measured using [123I] N-isopropyl p-iodoamphetamine/SPECT. The associations among clinical factors, disease severity, regional cerebral blood flow (rCBF), and intelligence test scores were also examined. RESULTS: The mean full-scale intelligence quotient (FIQ) was 101.8 ± 12.5 (range 76-125) in children with no apparent brain lesions. A significant difference in the intelligence scale index score was observed, most frequently (42.9%) between working memory index (WMI) and verbal comprehension index (VCI; VCI - WMI > 11 points). Regional CBF was significantly reduced both in the left and right medial frontal cortices (left: 61.3 ± 5.3 ml/100 g/min, right 65.3 ± 5.3 ml/100 g/min; p < 0.001) compared to the cerebellum (77.8 ± 6.8 ml/100 g/min). There was a significant association of rCBF in the left dorsolateral prefrontal cortex (DLPFC) with FIQ (r = 0.46, p = 0.034), perceptual reasoning index (PRI; r = 0.44, p = 0.045), and processing speed index (PSI; r = 0.44, p = 0.045). There was an association between rCBF of the left medial frontal cortex and PSI (r = 0.49, p = 0.026). Age of onset, family history, ischemic symptoms, and angiographic severity were not associated with poor cognitive performance. CONCLUSIONS: Although average intellectual ability was not reduced in children with MMD, the association of reduced rCBF in the left DLPFC and medial frontal cortex with FIQ, PRI, and PSI suggests mild cognitive dysfunction due to cerebral hypoperfusion.
  • Sogo Oki, Masahito Kawabori, Hiroaki Motegi, Shigeru Yamaguchi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
    Internal medicine (Tokyo, Japan) 58 18 2699 - 2702 2019年09月15日 [査読有り][通常論文]
     
    Temporal muscle abscess in children usually occurs from acute otitis media, and rapid progression and concomitant infectious disease often make it easy to diagnose. We report a rare case of a nine-month-old infant who showed right temporal mass with no evidence of infection. Computed tomography showed an osteolytic round mass, and magnetic resonance imaging revealed heterogenous enhancement with a high apparent diffusion coefficient. Malignant tumor was first suspected, but an open biopsy revealed the swelling to be temporal muscle abscess. It should be noted that temporal abscess may mimic the features of a malignant tumor, and multiple examinations should be performed for an accurate diagnosis.
  • Taku Sugiyama, Toshitaka Nakamura, Yasuhiro Ito, Kikutaro Tokairin, Ken Kazumata, Naoki Nakayama, Kiyohiro Houkin
    World journal of surgery 43 9 2309 - 2319 2019年09月 [査読有り][通常論文]
     
    BACKGROUND: The 'gentle' handling of tissue (i.e., 'respect for tissue') is a fundamental aspect of surgical performance and learning. To date, there have been no methodological assessments that quantitatively measure 'gentleness.' Therefore, the aims of this study were (1) to propose a novel metric for gentle surgical maneuvers, (2) to validate the feasibility of this methodology, and (3) to explore safer surgical techniques through this methodology. METHODS: Using surgical video-based motion software, the motion of the carotid artery around plaque was analyzed and quantified during a carotid endarterectomy. Kinematic parameters (minimum and maximum acceleration, and maximum and mean velocity) were compared among the surgical tasks and techniques, as well as between novice and expert surgeons. RESULTS: The surgical tasks of dissecting the common carotid artery, passing the proximal vessel loops, and ligating vessels showed the highest absolute values of kinematic parameters. Dissections perpendicular to the line of the internal carotid artery tended to show higher kinematic parameters than those in the parallel direction, with blunt dissections typically higher than sharp dissections. The kinematic parameters of novice surgeons were significantly higher than those of experts, and receiver operating curve analysis showed a strong discriminative power. CONCLUSION: This study shows that tissue motion parameters could be a novel and feasible surrogate marker for the objective assessment on the 'gentleness' of surgical performance. Future studies should be performed to further elucidate the relationship on the direct correlation between tissue kinematic data and clinical outcomes or surgical adverse events.
  • Yasuhiro Morii, Toshiya Osanai, Tomoki Ishikawa, Kensuke Fujiwara, Takumi Tanikawa, Kiyohiro Houkin, Eiichi Kobayashi, Katsuhiko Ogasawara
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 28 8 2292 - 2301 2019年08月 [査読有り][通常論文]
     
    BACKGROUND AND PURPOSE: Although endovascular thrombectomy combined with recombinant tissue-type plasminogen activator is effective for treatment of acute ischemic stroke, regional disparities in implementation rates of those treatments have been reported. Drive and retrieve system, where a qualified neurointerventionist travels to another primary stroke center for endovascular thrombectomy, has been practiced in parts of Hokkaido, Japan. This study aims to simulate the cost effectiveness of the drive and retrieve system, which can be a method to enhance equality and cost effectiveness of treatments for acute ischemic stroke. MATERIALS AND METHODS: The number of patients who had acute ischemic stroke in 2015 is estimated. Those patients are generated according to the population distribution, and thereafter patient transport time is analyzed in the 3 scenarios (1) 60-minute drive scenario, (2) 90-minute drive scenario, in which the drive and retrieve system operates within 60-minute or 90-minute driving distance (3) without the system, using geographic information system. Incremental cost-effectiveness rate, quality-adjusted life years, and medical and nursing care costs are estimated from the analyzed transport time. FINDINGS: The incremental cost-effectiveness rate by implementing the system was dominant. Cost reductions of $213,190 in 60-minute drive scenario, and $247,274 in the 90-minute scenario were expected, respectively. Such benefits are the most significant in Soya, Emmon, Rumoi, and Kamikawahokubu medical areas. CONCLUSIONS: The drive and retrieve system could enhance regional equality and cost effectiveness of ischemic stroke treatments in Hokkaido, which can be achieved using existing resources. Further studies are required to clarify its cost effectiveness from hospital perspective.
  • Yoko Sato, Ken Kazumata, Eiji Nakatani, Kiyohiro Houkin, Yasuhiro Kanatani
    Stroke 50 8 1973 - 1980 2019年08月 [査読有り][通常論文]
     
    Background and Purpose- A public registration system for intractable diseases was started in Japan in 1972 to investigate the etiology and pathogenesis of intractable diseases while reducing out-of-pocket medical expenses on patients. The goal of this study was to investigate the epidemiology and clinical characteristics of Moyamoya disease using data from applications submitted to this system between 2004 and 2008. Methods- In addition to demographic factors such as onset age and family history, we evaluated clinical presentation type, imaging findings, clinical symptoms, and functioning in activities of daily living (ADL). Results- Of 3859 cases for which applications were submitted, 2545 were confirmed to meet the diagnostic criteria after data cleansing. Onset age showed a bimodal distribution, and Moyamoya disease had a higher incidence in women than in men. The presence of occlusion and infarction in the proximal region of the anterior cerebral artery was more frequent in pediatric cases than adult cases. Our findings also indicated that 23% of patients required assistance with ADL. Cerebral infarction (odds ratio [OR], 12.5; 95% CI, 3.55-44.66), seizure (OR, 7.44; 95% CI, 1.29-42.96), and sensory disorders (OR, 5.23; 95% CI, 1.15-23.75) were identified as significant predictors of impaired ADL in pediatric cases 3 years after the initial application. Moderate ADL function (OR, 11.59; 95% CI, 5.29-25.39) and intellectual disabilities (OR, 4.38; 95% CI, 1.58-12.17) at the time of the application were identified as significant prognostic factors in adults. Conclusions- The results of this study indicated that characteristics of Moyamoya disease such as onset type, symptoms, and imaging abnormalities differ with onset age. Prognostic analyses suggested that pediatric cases with good ADL but with infarct type onset, seizure, or sensory disorders might have a subsequent decline in ADL.
  • Toshitaka Seki, Shuji Hamauchi, Masayoshi Yamazaki, Kazutoshi Hida, Shunsuke Yano, Kiyohiro Houkin
    Asian spine journal 13 4 648 - 653 2019年08月 [査読有り][通常論文]
     
    Study Design: Retrospective cohort study. Purpose: To investigate the correlation between the syrinx morphology and neuropathic pain caused by syringomyelia associated with Chiari I malformation. Overview of Literature: Neuropathic pain caused by syringomyelia is refractory and markedly impairs the patient. Methods: We examined 24 patients with neuropathic pain caused by syringomyelia associated with Chiari I malformation. We statistically analyzed the illness duration and age at surgery between patients with and without neuropathic pain. Additionally, we classified the morphology of the syringes into deviated (D), enlarged (E), central (C), and bulkhead (B) types using T2-weighted axial imaging. Moreover, we investigated the correlation between syrinx morphology and neuropathic pain. A Mann-Whitney U-test was performed to compare between the presence or absence of neuropathic pain and the presence or absence of type D syringes. Results: The median age at surgery was 27.5 years, and the median illness duration was 24 months. Among the 24 patients, 11 had preoperative neuropathic pain, one of which was free of neuropathic pain during the final follow-up period. Among patients with neuropathic pain, the syringes' preoperative morphology was type D in nine patients and types E and C in one patient each. No patient exhibited type B morphology. Among patients without neuropathic pain, the preoperative morphology of the syringes was type D in three patients, type E in seven patients, and types C and B in two patients each. For types D and E, a correlation between neuropathic pain and syrinx morphology was observed. Moreover, type D was associated with significant neuropathic pain in both preoperative and postoperative states. Conclusions: This study showed a correlation between the morphological features of the syringes and the occurrence of neuropathic pain in patients with syringomyelia associated with Chiari I malformation.
  • Toshiya Osanai, Yasuhiro Ito, Satoshi Ushikoshi, Takeshi Aoki, Masahito Kawabori, Kensuke Fujiwara, Katsuhiko Ogasawara, Kikutaro Tokairin, Katsuhiko Maruichi, Naoki Nakayama, Ken Kazumata, Kota Ono, Kiyohiro Houkin
    Journal of neurointerventional surgery 11 8 757 - 761 2019年08月 [査読有り][通常論文]
     
    BACKGROUND: Outcomes of endovascular treatment for acute ischemic stroke depend on the time interval from onset to reperfusion. Although the centralized 'mothership' method is considered preferable, the required transportation time increases the risk that a patient with a stroke may not receive intravenous or endovascular therapy. In contrast, 'drive and retrieve' describes a system wherein doctors from comprehensive stroke centers travel to primary stroke centers and provide endovascular treatment for acute ischemic stroke. OBJECTIVE: To describe the drive and retrieve system and verify the effects of this new collaboration on outcomes in patients with acute ischemic stroke among facilities. METHODS: This non-randomized, single-arm study retrospectively analyzed patients who met the inclusion criteria for endovascular treatment provided through a drive and retrieve system. Among the 122 patients treated by this system, we analyzed the time of onset to recanalization as the primary outcome. We also analyzed the efficacy of the drive and retrieve system using geographic information system analysis. RESULTS: The median time from onset to recanalization was 229 min (IQR 170-307 min, 95% CI 201 to 252 min). The upper limit of the 95% CI for the time from onset to recanalization was shorter than the median times reported in two previous trials. Geographic information system analysis revealed an upward trend in the population coverage rate in each secondary medical area after the drive and retrieve method was introduced. CONCLUSION: The drive and retrieve method may be an effective form of cooperation between facilities located within 1 hour of a comprehensive stroke center.
  • Kikutaro Tokairin, Toshiya Osanai, Ken Kazumata, Ryosuke Sawaya, Kiyohiro Houkin
    World neurosurgery 127 79 - 84 2019年07月 [査読有り][通常論文]
     
    BACKGROUND: Middle meningeal arteriovenous fistula (MM-AVF) is rare; however, it will sometimes be followed by intracranial hemorrhage or progressive symptoms caused by abnormal shunt flow. Radiological examination and endovascular treatment of this condition have recently advanced; thus, we have described the pathogenesis, clinical features, and appropriate diagnostic and therapeutic management of MM-AVF. We also reviewed the reported data of the past 35 years, including 30 cases of MM-AVF. CASE DESCRIPTION: We report the case of 24-year-old man who had presented with right tinnitus who had experienced previous head trauma on the opposite side to the tinnitus ear. Time-of-flight magnetic resonance angiography and magnetic resonance arterial spin labeling findings were suggestive of MM-AVF, and catheter angiography confirmed MM-AVF with shunt flow draining into the cavernous sinus. Endovascular transarterial embolization was performed, and the MM-AVF was embolized successfully using detachable coils and n-butyl-2-cyanoacrylate. The tinnitus disappeared completely immediately after the treatment. CONCLUSIONS: MM-AVF is caused, not only by coup injury, but also by contrecoup injury. Time-of-flight magnetic resonance angiography and magnetic resonance arterial spin labeling are useful for detecting MM-AVF. Endovascular transarterial embolization is an effective and safe treatment.
  • Yukitomo Ishi, Shigeru Yamaguchi, Michiharu Yoshida, Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
    Journal of neuroradiology = Journal de neuroradiologie 48 4 266 - 270 2019年06月20日 [査読有り][通常論文]
     
    BACKGROUND AND PURPOSE: Most individuals with optic pathway/hypothalamic pilocytic astrocytoma (OPHPA) harbor either the BRAF V600E mutation or KIAA1549-BRAF fusion (K-B). This study aimed to investigate the imaging characteristics of OPHPA in relation to BRAF alteration status. MATERIALS AND METHODS: Seven cases of OPHPA harboring either the BRAF V600E mutation or K-B fusion were included in the study. Preoperative magnetic resonance imaging (MRI) was assessed for degree of T2 hyperintensity on T2-weighted images (T2WI) and the ratio of nonenhancing T2 or fluid-attenuated inversion recovery (FLAIR) hyperintense area to the contrast enhanced area (CE) on gadolinium-enhanced-T1 weighted images (T2/FLAIR-CE mismatch). The T2 signal intensity was normalized to cerebrospinal fluid (T2/CSF) for both the V600E and K-B group and compared. T2/FLAIR-CE mismatch was assessed by calculating the proportion of the tumor volume of nonenhancing high T2 signal intensity to the whole lesion (nonenhancing and enhancing components). RESULTS: Four and three cases of OPHPA harboring the BRAF V600E mutation and K-B, respectively, were analyzed. The T2/CSF value was higher in the K-B group than in the V600E group. Moreover, the V600E group had a larger T2/FLAIR-CE mismatch than the K-B group. CONCLUSIONS: The BRAF alteration status in individuals with OPHPA was associated with preoperative MRI by focusing on T2 signal intensity and T2/FLAIR-CE mismatch. The BRAF V600E mutation was associated with a lower T2/CSF value and larger T2/FLAIR-CE mismatch, whereas K-B fusion was associated with a higher T2/CSF value and smaller T2/FLAIR-CE mismatch.
  • Kazunori Toyoda, Shinichiro Uchiyama, Takenori Yamaguchi, J Donald Easton, Kazumi Kimura, Haruhiko Hoshino, Nobuyuki Sakai, Yasushi Okada, Kortaro Tanaka, Hideki Origasa, Hiroaki Naritomi, Kiyohiro Houkin, Keiji Yamaguchi, Masanori Isobe, Kazuo Minematsu
    The Lancet. Neurology 18 6 539 - 548 2019年06月 [査読有り][通常論文]
     
    BACKGROUND: Although dual antiplatelet therapy with aspirin and clopidogrel reduces early recurrence of ischaemic stroke, with long-term use this type of therapy is no longer effective and the risk of bleeding increases. Given that cilostazol prevents stroke recurrence without increasing the incidence of serious bleeding compared with aspirin, we aimed to establish whether dual antiplatelet therapy involving cilostazol is safe and appropriate for long-term use. METHODS: In a multicentre, open-label, randomised controlled trial across 292 hospitals in Japan, patients with high-risk non-cardioembolic ischaemic stroke identified on MRI were randomly assigned to two groups in a 1:1 ratio to receive monotherapy with either oral aspirin (81 or 100 mg, once per day) or clopidogrel (50 or 75 mg, once per day) alone, or a combination of cilostazol (100 mg, twice per day) with aspirin or clopidogrel. Randomisation was done centrally (using block randomisation with a block size of six per each participating hospital) through a web-based registration system and was done by EPS Corporation. The patients were required to have at least 50% stenosis of a major intracranial or extracranial artery or two or more of the vascular risk factors. Trial medication was continued for half a year or longer, for a maximum of 3·5 years. The primary efficacy outcome was the rate of first recurrence of symptomatic ischaemic stroke. Safety outcomes were severe or life-threatening bleeding; any adverse events; serious adverse events; and any bleeding events. Efficacy analyses were done in the intention-to-treat population and safety analyses were done in the as-treated population. This trial was registered with ClinicalTrials.gov (number NCT01995370) and UMIN Clinical Trials Registry (number 000012180). FINDINGS: Participants were recruited from Dec 13, 2013, to March 31, 2017. 932 patients assigned to the dual therapy group and 947 patients assigned to the monotherapy group were included in the intention-to-treat analysis. The trial was stopped after the enrolment of 1884 patients of an anticipated 4000 patients because of the delay in recruitment. Ischaemic stroke recurred in 29 (3%) of 932 patients (annualised rate 2·2%) on dual therapy including cilostazol and 64 (7%) of 947 patients (annualised rate 4·5%) on monotherapy during a median 1·4 years follow-up (hazard ratio [HR] 0·49, 95% CI 0·31-0·76, p=0·0010). Severe or life-threatening bleeding occurred in eight patients (annualised rate 0·6%) on dual therapy and 13 patients (annualised rate 0·9%) on monotherapy (HR 0·66, 95% CI 0·27-1·60, p=0·35). Occurrence of any type of adverse event was similar between the groups (255 [28%] of 910 patients in the dual therapy group vs 219 [24%] of 921 patients in the monotherapy group); as was occurrence of serious adverse events (87 [10%] vs 142 [15%]) and bleeding events (38 [4%] vs 33 [4%]). Gastrointestinal bleeding, which affected nine (<1%) of 910 patients in the monotherapy group and nine (<1%) of 921 patients in the dual therapy group, was the most common type of bleeding. INTERPRETATION: The combination of cilostazol with aspirin or clopidogrel had a reduced incidence of ischaemic stroke recurrence and a similar risk of severe or life-threatening bleeding compared with treatment with aspirin or clopidogrel alone in patients at high risk for recurrent ischaemic stroke. FUNDING: Otsuka Pharmaceutical.
  • Chongxian Hou, Shigeru Yamaguchi, Yukitomo Ishi, Shunsuke Terasaka, Hiroyuki Kobayashi, Hiroaki Motegi, Kanako C Hatanaka, Kiyohiro Houkin
    Journal of neuro-oncology 143 2 197 - 206 2019年06月 [査読有り][通常論文]
     
    PURPOSE: 5-aminolevulinic acid (5-ALA) fluorescence-guided surgery (FGS) appears to be a promising treatment for glioma. However, 5-ALA-mediated fluorescence cannot always be detected in grade II/III gliomas. We hypothesized that gene expression patterns in the Protoporphyrin IX (PpIX) synthesis pathway may be associated with intraoperative fluorescence status of grade II/III gliomas, and then attempted to identify the key molecule of 5-ALA-mediated fluorescence. METHODS: Using 50 surgically obtained specimens, which were diagnosed as grade II and III gliomas, we analyzed gene expression within the PpIX synthesis pathway to identify candidate molecules according to intraoperative 5-ALA-mediated fluorescence status. The most likely candidate gene was selected and confirmed by protein expression analysis. To evaluate the biological function of the molecule in PpIX synthesis, functional analysis was performed using specific, small interference (si)RNA in the SW-1783 human grade III glioma cell line. RESULTS: Among the genes involved in the porphyrin synthesis pathway, the mRNA expression of Peptide transporter 2 (PEPT2) in FGS fluorescence-positive gliomas was significantly higher than that in fluorescence-negative gliomas. Protein expression of PEPT2 was also significantly higher in the fluorescence-positive gliomas, which was confirmed by western blot analysis and immunofluorescence analysis. The siRNA-mediated downregulation of the mRNA and protein expression of PEPT2 led to decreased PpIX fluorescence intensity, as confirmed by fluorescence spectrum analysis. CONCLUSIONS: The results suggest PEPT2 is an important candidate molecule in 5-ALA-mediated FGS in grade II/III gliomas. As the overexpression of PEPT2 was associated with higher PpIX fluorescence intensity, PEPT2 may improve fluorescence-guided resection in grade II/III gliomas.
  • Haruto Uchino, Daina Kashiwazaki, Naoki Akioka, Masaki Koh, Naoya Kuwayama, Kiyohiro Houkin, Satoshi Kuroda
    Journal of neurosurgery 132 6 1 - 11 2019年05月31日 [査読有り][通常論文]
     
    OBJECTIVE: In this study the authors aimed to describe clinical features, surgical techniques, and long-term outcomes of repeat bypass surgery required for a certain subset of patients with moyamoya disease. METHODS: The authors retrospectively reviewed a total of 22 repeat bypass surgeries for 20 patients (age range 1-69 years) performed during the last 20 years at their institutions. The patients were classified into 2 groups. Group A included 10 patients who underwent repeat bypass surgery for anterior circulation due to insufficient revascularization on the ipsilateral side. Group B included 10 patients who underwent repeat bypass surgery for posterior circulation due to the involvement of the posterior cerebral artery (PCA) after successful initial surgery for anterior circulation. RESULTS: Preoperative symptoms included headache in 3 patients, transient ischemic attack in 10, cerebral infarction in 3, and intracranial hemorrhage in 4 patients. Intervals between the initial bypass surgery and repeat bypass surgery were 0.3-30 years (median 3 years). In group A, superficial temporal artery to middle cerebral artery (MCA) anastomosis and indirect bypass were performed on 7 hemispheres. Only indirect bypass was performed on 3 hemispheres because of the lack of suitable donor or recipient arteries. In group B, occipital artery (OA) to PCA anastomosis and indirect bypass were conducted on 4 hemispheres, and OA-MCA anastomosis and indirect bypass on 1 hemisphere. Only indirect bypass was conducted on 7 hemispheres because of the lack of suitable recipient arteries. All 22 repeat bypass surgeries were successfully conducted. During follow-up periods (median 4 years), none of the patients suffered repeat stroke except 1 patient who died of recurrent intracerebral hemorrhage 3 years after repeat bypass surgery for anterior circulation. CONCLUSIONS: Repeat bypass surgery was feasible and effective to reduce further incidence of headache attack, transient ischemic attack, and ischemic/hemorrhagic stroke in moyamoya disease patients. Through precise radiological analysis, surgical procedures should be planned to yield maximal therapeutic effects.
  • Miki Fujimura, Takeshi Funaki, Kiyohiro Houkin, Jun C Takahashi, Satoshi Kuroda, Yasutake Tomata, Teiji Tominaga, Susumu Miyamoto
    Journal of neurosurgery 130 5 1453 - 1459 2019年05月01日 [査読有り][通常論文]
     
    OBJECTIVE: This study was performed to identify the angiographic features of hemorrhagic-onset moyamoya disease (MMD) in comparison with those of patients with ischemic-onset MMD. METHODS: This case-control study compared the data set of the Japan Adult Moyamoya (JAM) Trial with the angiographic data of adult patients with ischemic-onset MMD. The authors analyzed angiograms obtained at onset, classifying the collaterals into 3 subtypes: lenticulostriate anastomosis, thalamic anastomosis, and choroidal anastomosis. They then compared the extent of these collaterals, as indicated by the collateral development grade from 0 to 2 in each subtype, between the JAM Trial group and the ischemic-onset group. They also compared the involvement of the posterior cerebral artery (PCA) and Suzuki's angiographic staging between each group. RESULTS: Among 89 ischemic-onset patients, 103 symptomatic hemispheres in 80 patients were analyzed and compared with 75 hemorrhagic hemispheres from the JAM Trial. The hemorrhagic-onset patients showed a significantly higher proportion of thalamic anastomosis (p = 0.043) and choroidal anastomosis (< 0.001), as indicated by grade 2 in each subtype, compared with ischemic-onset patients. Suzuki's angiographic staging was significantly higher in the hemorrhagic group (< 0.038). There was no difference in the extent of lenticulostriate anastomosis and PCA involvement between the groups. CONCLUSIONS: In adult MMD, the characteristic pattern of the abnormal vascular networks at the base of the brain is different between each onset type. In light of the more prominent development of thalamic and choroidal anastomosis in the JAM Trial group in the present study, development of these collaterals, especially the choroidal collateral extending beyond the lateral ventricle, may play a critical role in hemorrhagic presentation in MMD.Clinical trial registration no. C000000166 (http://www.umin.ac.jp/ctr/index.htm).
  • Masahito Kawabori, Kota Kurisu, Yoshimasa Niiya, Yuzuru Ohta, Shoji Mabuchi, Kiyohiro Houkin
    Internal medicine (Tokyo, Japan) 58 8 1163 - 1166 2019年04月15日 [査読有り][通常論文]
     
    A rare case of Mollaret meningitis characterized by four recurrent episodes of aseptic meningitis during a three-year period is reported. The patient showed a high fever and severe headache accompanied by a high level of cerebrospinal fluid (CSF) cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). The symptoms and high CSF cytokines were resolved immediately after introducing indomethacin treatment. Reactivation of the latent virus is considered to be the cause of this rare disease, and indomethacin is believed to inhibit the periodic abnormal generation of eicosanoid in the brain, resulting in a reduction in the fever and subsequent inflammation.
  • Soichiro Takamiya, Toshiya Osanai, Toshitaka Seki, Noriyuki Fujima, Kazutoshi Hida, Takeshi Asano, Kazuyoshi Yamazaki, Shuji Hamauchi, Toru Sasamori, Kota Ono, Shunsuke Terasaka, Kiyohiro Houkin
    European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 28 4 842 - 848 2019年04月 [査読有り][通常論文]
     
    PURPOSE: Spinal angiography is the gold standard for evaluation or diagnosis of spinal arteriovenous malformations (AVMs). However, some feeding arteries might be overlooked when multiple feeders exist. This study aimed to retrospectively review cases of spinal intra-dural AVMs, which were identified by three-dimensional digital subtraction angiography (3D-DSA), and attempted to estimate the number of feeding arteries. METHODS: We retrospectively reviewed patients with spinal intra-dural AVMs who underwent 3D-DSA at Hokkaido University Hospital from January 2005 to December 2016. We selected 9 patients in whom we could obtain data of multi-planar reconstruction of 3D-DSA. We measured the computed tomography (CT) values of feeding arteries and draining veins. The CT values represented the averages of maximum CT values of 5 continuous axial slices. The ratio of the CT value of feeders to that of drainers (F/D ratio) was calculated. The correlation between the F/D ratio and the number of feeders was examined with Pearson's correlation coefficient. RESULTS: The average number of feeders was 2.3 (1-4), and the number of feeders was significantly positively correlated with the F/D ratio (r = 0.855, P = .003). CONCLUSIONS: We conclude that the number of feeding arteries of spinal intra-dural AVMs can be estimated by using the F/D ratio obtained from 3D-DSA. These slides can be retrieved under Electronic Supplementary Material.
  • Takeshi Funaki, Jun C Takahashi, Kiyohiro Houkin, Satoshi Kuroda, Miki Fujimura, Yasutake Tomata, Susumu Miyamoto
    Journal of neurosurgery 132 2 408 - 414 2019年02月08日 [査読有り][通常論文]
     
    OBJECTIVE: Following hemorrhagic stroke in moyamoya disease, de novo intracranial hemorrhage can occur in the previously unaffected nonhemorrhagic hemisphere. In the present analysis the authors intended to determine whether the presence in the nonhemorrhagic hemisphere of choroidal collateral vessels, which have been the focus of attention as a source of bleeding, affects the risk of de novo hemorrhage. METHODS: The subject of focus of the present cohort study was the nonhemorrhagic hemispheres of adult patients with hemorrhagic moyamoya disease enrolled in the Japan Adult Moyamoya Trial and allocated to the nonsurgical arm. The variable of interest was the presence of choroidal collaterals (also termed choroidal anastomoses), identified with baseline angiography and represented by a connection (anastomosis) between the anterior or posterior choroidal arteries and the medullary arteries. The outcome measure was de novo hemorrhage during the 5-year follow-up period, assessed in all nonhemorrhagic hemispheres. The incidence of de novo hemorrhage in the collateral-positive and -negative groups was compared. RESULTS: Choroidal collaterals were present in 15 of 36 (41.7%) nonhemorrhagic hemispheres analyzed. The overall annual risk of de novo hemorrhage was 2.0%. Three de novo hemorrhages occurred in the collateral-positive group, whereas no hemorrhage occurred in the collateral-negative group. The annual risk of de novo hemorrhage was significantly higher in the collateral-positive group than in the collateral-negative group (5.8% per year vs 0% per year; p = 0.017). All hemorrhage sites corresponded to the distribution of choroidal collaterals. CONCLUSIONS: The present preliminary results suggest that the presence of choroidal collaterals affects the risk of de novo hemorrhage in the nonhemorrhagic hemisphere, subject to verification in larger studies. Further studies are needed to determine the optimal treatment strategy for nonhemorrhagic hemispheres and asymptomatic patients.
  • Masahiro Yasaka, Kazuo Minematsu, Kazunori Toyoda, Etsuro Mori, Teruyuki Hirano, Toshimitsu Hamasaki, Hiroshi Yamagami, Takehiko Nagao, Shinichi Yoshimura, Shinichiro Uchiyama, Takenori Yamaguchi, Yasushi Okada, Satoshi Okuda, Kazumi Kimura, Norio Tanahashi, Yasuo Terayama, Yoichiro Hashimoto, Yasuhiro Hasegawa, Kiyohiro Houkin, Masayasu Matsumoto, Kazuo Kitagawa, Masahiro Yasaka, Ken Nagata, Shigeru Nogawa, Yoshiaki Kumon, Takeshi Kimura, Yutaka Furukawa, Tomohiro Sakamoto, Makoto Sakai, Kohsuke Kudo, Toshinori Hirai, Shotai Kobayashi
    PLOS ONE 14 2 2019年02月 [査読有り][通常論文]
     
    The efficacy of early anticoagulation in acute stroke with nonvalvular atrial fibrillation (NVAF) remains unclear. We performed a study to evaluate the risk of recurrent ischemic stroke (IS) and major bleeding in acute IS patients with NVAF who started rivaroxaban. This observational study evaluated patients with NVAF and acute IS/transient ischemic attack (TIA) in the middle cerebral arterial territory who started rivaroxaban within 30 days after the index IS/TIA. The primary endpoints were recurrent IS and major bleeding within 90 days after the index IS/TIA. The relationship between the endpoints and the time to start rivaroxaban was evaluated by correlation analysis using cerebral infarct volume, determined by diffusion-weighted magnetic resonance images within 48 hours of onset of the index IS/TIA. Of 1309 patients analyzed, recurrent IS occurred in 30 (2.3%) and major bleeding in 11 (0.8%) patients. Among patients with known infarct size (N = 1207), those with small (<4.0 cm(3)), medium (>= 4.0 and <22.5 cm(3)), and large (>= 22.5 cm(3)) infarcts started rivaroxaban a median of 2.9, 2.9, and 5.8 days, respectively, after the index IS/TIA. Recurrent IS was significantly less frequent when starting rivaroxaban <= 14 days versus >= 15 days after IS (2.0% versus 6.8%, P=0.0034). Incidences of recurrent IS and major bleeding in whom rivaroxaban was started <3 days (N = 584) after IS were also low: 1.5% and 0.7%, respectively. Initiation of rivaroxaban administration in acute IS or TIA was associated with a low recurrence of IS (2.3%), and a low incidence of major bleeding events (0.8%) for 90 days after the index stroke. For the prevention of recurrent attacks in acute IS patients with NVAF, it is feasible to start the administration of rivaroxaban within 14 days of onset. Rivaroxaban started within 3 days of onset may be a feasible treatment option for patients with a small or medium-sized infarction.
  • Takeshi Funaki, Jun C Takahashi, Kiyohiro Houkin, Satoshi Kuroda, Shigekazu Takeuchi, Miki Fujimura, Yasutake Tomata, Susumu Miyamoto
    Journal of neurosurgery 130 2 337 - 673 2019年02月01日 [査読有り][通常論文]
     
    OBJECTIVEChoroidal collateral vessels typical of moyamoya disease have received attention as a potential bleeding source. The authors' previous angiographic cross-sectional analysis suggested a possible association between choroidal collaterals and posterior hemorrhage, indicating a high risk for rebleeding. The present longitudinal analysis is intended to determine whether choroidal collaterals are a predictor of rebleeding in hemorrhagic moyamoya disease.METHODSThe Japan Adult Moyamoya Trial group designed an ancillary cohort study using 5-year follow-up data on 37 patients included in the nonsurgical arm of the original randomized controlled trial and compared the rebleeding rate of those with and those without choroidal collaterals, represented by the connection between the anterior or posterior choroidal arteries and the medullary arteries. An expert panel determined whether a choroidal collateral was present in each patient through the measurement of baseline angiography studies. The rebleeding rate comparison was adjusted for age, diagnosis of hypertension, and involvement of the posterior cerebral artery.RESULTSChoroidal collaterals were present in 21 patients (56.8%). The rebleeding rate was 13.1% per year in the collateral-positive group as compared with 1.3% in the negative group (p = 0.008, log-rank test). The adjusted hazard ratio for rebleeding in the collateral-positive group relative to the negative group remained statistically significant (HR 11.10, 95% CI 1.37-89.91). Radiographic assessment of the collateral-positive group revealed good correspondence between the distribution of collaterals and rebleeding sites.CONCLUSIONSResults of this study suggest that choroidal collaterals are a bleeding source with a high risk for hemorrhagic recurrence and a predictor of rebleeding in hemorrhagic moyamoya disease.
  • Hiroyuki Kobayashi, Shigeru Yamaguchi, Hiroaki Motegi, Sadahiro Kaneko, Shogo Endou, Rikiya Onimaru, Shunsuke Terasaka, Kiyohiro Houkin
    Journal of chemotherapy (Florence, Italy) 31 1 35 - 41 2019年02月 [査読有り][通常論文]
     
    High-dose methotrexate (HD-MTX)-based chemotherapy in combination with whole brain radiotherapy (WBRT) has been a common therapy for primary central nervous system lymphoma (PCNSL). The aim of this study was to evaluate the survival benefit of a minimized cycle of HD-MTX monotherapy prior to WBRT. A maximum of three cycles of HD-MTX was combined with a WBRT dose of 30 Gy and an additional localized boost was administered where remnant was observed. A total of 54 patients with newly diagnosed PCNSL were enrolled in this study. The objective response rate for HD-MTX was 80% and the median overall survival was 58.4 months. Responders to HD-MTX demonstrated better survival than patients with resistance. The concentration of MTX in serum and cerebrospinal fluid was not related the chemotherapeutic response. This study demonstrated the efficacy of HD-MTX prior to WBRT and indicated that three cycles of HD-MTX monotherapy may be sufficient in combination with radiotherapy.
  • Michiharu Yoshida, Hiroyuki Kobayashi, Shunsuke Terasaka, Shogo Endo, Shigeru Yamaguchi, Hiroaki Motegi, Rachmilevitch Itay, Shiji Suzuki, Omer Brokman, Yeruham Shapira, Kenji Moriyama, Yumiko Kawase, Toshiaki Akahane, Yasutaka Kato, Hajime Kamada, Kiyohiro Houkin
    ULTRASOUND IN MEDICINE AND BIOLOGY 45 2 526 - 538 2019年02月 [査読有り][通常論文]
     
    Sonodynamic therapy (SDT) is used to treat various malignancies and can be applied to brain tumors using a transcranial magnetic resonance imaging-guided focused ultrasound (TcMRgFUS) device. This study investigated the efficacy of 220-kHz TcMRgFUS combined with 5-aminolevulinic acid (5-ALA) on malignant glioma in vitro and in vivo. F98 cells were irradiated with focused ultrasound (FUS) (4000 J, 20 W, 240 s, 100% duty cycle, target medium temperature <40 degrees C) after treatment with 200 mu g/mL 5-ALA, and cell viability and apoptosis were evaluated with the water-soluble tetrazolium-1 assay, triple fluorescent staining and Western blot analysis 20 h later. The anti-tumor effects of 5-ALA combined with FUS (500 J, 18 W, 30 s, 100% duty cycle, 10 repeats, target tissue temperature <= 42 degrees C) were assessed on the basis of changes in tumor volume determined by MRI and histopathological analysis before and after treatment. The FUS/5-ALA combination reduced cell viability by inducing apoptosis and suppressed tumor proliferation and invasion as well as angiogenesis in vivo, while causing minimal damage to normal brain tissue. SDT with 220-kHz TcMRgFUS and 5-ALA can be safely used for the treatment of malignant glioma. (E-mail: hiro-ko@med.hokudai.ac.jp) (C) 2018 World Federation for Ultrasound in Medicine & Biology. All rights reserved.
  • 山崎和義, 川堀真人, 関俊隆, 七戸秀夫, 高宮宗一郎, 濱内祝嗣, 寳金清博, 寳金清博
    日本分子脳神経外科学会プログラム・抄録集 20th 2019年
  • 川掘真人, 七戸秀夫, 黒田敏, 寳金清博
    脳循環代謝(Web) 31 1 2019年
  • 山崎和義, 川堀真人, 関俊隆, 濱内祝嗣, 寳金清博
    日本脊髄外科学会プログラム・抄録集 34th 2019年
  • 高宮宗一朗, 関俊隆, 濱内祝嗣, 山崎和義, 飛彈一利, 小柳泉, 寳金清博
    日本脊髄外科学会プログラム・抄録集 34th 2019年
  • 寳金清博
    もやもや病(ウイリス動脈輪閉塞症)の診断、治療に関する研究 平成30年度 総括研究報告書(Web) 2019年
  • 数又研, 寳金清博
    もやもや病(ウイリス動脈輪閉塞症)の診断、治療に関する研究 平成30年度 総括研究報告書(Web) 2019年
  • 濱内祝嗣, 関俊隆, 森田亮, 木村輔, 阿保大介, 寳金清博
    日本脊髄外科学会プログラム・抄録集 34th 2019年
  • 東海林菊太郎, 長内俊也, 藤間憲幸, 牛越聡, 茂木洋晃, 伊師雪友, 寳金清博
    脳血管内治療(Web) 4 Supplement 2019年
  • 長内俊也, 東海林菊太郎, 今井哲秋, 寳金清博, 小笠原克彦
    脳血管内治療(Web) 4 Supplement 2019年
  • 伊師雪友, 関俊隆, 高宮宗一郎, 山崎和義, 濱内祝嗣, 山口秀, 寳金清博, 飛騨一利, 石田雄介, 小田義崇, 田中仲哉, 茂木洋晃, 岡本迪成
    日本脳腫瘍学会プログラム・抄録集 37th 2019年
  • Kikutaro Tokairin, Ken Kazumata, Haruto Uchino, Masaki Ito, Kota Ono, Ryota Tatezawa, Takafumi Shindo, Masahito Kawabori, Naoki Nakayama, Kiyohiro Houkin
    World neurosurgery 120 e593-e600 - e600 2018年12月 [査読有り][通常論文]
     
    OBJECTIVE: In combined revascularization surgery for patients with moyamoya disease, intracerebral hemorrhage (ICH) during the postoperative acute phase is a rarely observed but severe complication. Its clinical features remain unclear because of its low incidence rate. The aim of this study was to clarify the clinical characteristics of immediate postoperative ICH. METHODS: The frequency, onset timing, and hematoma location of patients who demonstrated immediate postoperative ICH were investigated in 201 consecutive surgeries performed in 134 patients. Associations between immediate postoperative ICH and demographics, clinical presentation type, perioperative blood pressure (BP), and neuroimaging data were analyzed. RESULTS: Postoperative ICH was observed in 6 cases (2.99%; mean age, 46.0 ± 7.6 years). The onset timing of ICH was within 24 hours after surgery in most patients (83.3%). Hematomas were located at the subcortical lesion beneath the anastomosed cortex (n = 5) and caudate head (n = 1). Three cases (50.0%) required hematoma evacuation. A higher age at surgery was associated with postoperative ICH (P = 0.046). In adult cases (132 surgeries, 65.7%), hemorrhagic presentation at onset (P = 0.0027) and an increase in BP from pre- to postoperative stage (systolic BP increase: P = 0.0058, diastolic BP increase: P = 0.0274) were significantly associated with postoperative ICH. CONCLUSIONS: The results suggest that older patients, with hemorrhagic presentation and greater postoperative BP increase, should be carefully managed to avoid postoperative ICH. Immediate hematoma evacuation may be effective in preventing devastating outcomes after postoperative ICH.
  • Daisuke Oura, Masahito Kawabori, Yoshimasa Niiya, Motoyuki Iwasaki, Shinpei Satoh, Takumi Yokohama, Shoji Mabuchi, Kiyohiro Houkin
    Journal of neurosurgical sciences 2018年11月21日 [査読有り][通常論文]
     
    BACKGROUND: Recent clinical trials demonstrated the efficacy of thrombectomy for ischemic stroke against acute large vessel occlusion (LVO). To overcome the problem with excessive examination time for diagnosis of cerebral perfusion and/or the use of contrast agent to determine penumbra, we adopted a new magnetic resonance imaging technique named Acute Stroke Assessment using rapid Pseudo-continuous arterial spin labeling (ASAP-ASL) method. METHODS: The study included healthy volunteers and clinical patients. The signal to noise ratio (SNR) and acquisition time were compared with various numbers of signal average (NSA) of rapid pseudo-continuous arterial spin labeling (pCASL) using the 10-mm thick slice width and narrow scan range focusing the level of basal ganglia by healthy volunteers. After applying clinically acceptable protocol for ASAP-ASL, we then checked image qualities and an accuracy of the method by comparing with the angiographical imaging obtained from the clinical patients regarding the degree of consistency. RESULTS: NSA were compared between two and fourteen, and 10 NSA was decided to be introduced for clinical use (1 minutes and 17 second) for obtaining clinically acceptable image, which was shorter than the time required for ordinary whole brain pCASL (approximately 5 minutes). In the clinical study, the occlusion site estimated by ASAP-ASL showed high correlation with that of digital subtraction angiography (κ = 0.63-0.79). CONCLUSIONS: ASAP-ASL method requires approximately one minutes to obtain clinically relevant brain perfusion imaging which can successfully identify ischemic region in LVO patients.
  • Shuji Hamauchi, Toshiya Osanai, Toshitaka Seki, Masahito Kawabori, Michinari Okamoto, Kazutoshi Hida, Kiyohiro Houkin
    Journal of neurosurgery. Spine 29 5 576 - 581 2018年11月01日 [査読有り][通常論文]
     
    The authors describe a novel method of observing blood flow in abnormal vessels with slow-motion video during surgical treatment of spinal arteriovenous shunts. The method is based on the use of superselective angiography with saline for visualizing abnormal vessels in bright field and commercially available high frame rate digital camera for recording slow-motion video.
  • Toshiya Osanai, Ken Kazumata, Satoshi Kobayashi, Noriyuki Fujima, Kota Kurisu, Yuusuke Shimoda, Kiyohiro Houkin
    World neurosurgery 119 237 - 241 2018年11月 [査読有り][通常論文]
     
    BACKGROUND: Electrocardiogram (ECG)-triggered angiography non-contrast-enhanced (TRANCE) imaging is useful for investigating peripheral vessel diseases; however, its efficacy for access route assessment in cerebral angiography has yet to be reported. Therefore we aimed to evaluate the efficacy of TRANCE imaging in the assessment of the access route before diagnostic subtraction angiography for cerebral vascular disorders. METHODS: TRANCE imaging was performed in all patients undergoing catheter angiography for disease diagnosis at our institute between April 2014 and March 2015. This study included 31 patients (14 men, 17 women) and investigated potential changes in the planned puncture site before and during the procedure (including the reason for the change) as our main outcome. RESULTS: TRANCE was successfully conducted in all of the patients recruited. TRANCE images led to an alteration in the approach vessel for 5 cases. The mean procedure time for angiography was <60 minutes. The median total number of catheters and guidewires used during catheter angiography was 1 (range: 1-3) and 1 (range: 1-2), respectively. There were no catheter angiography-related complications. CONCLUSIONS: TRANCE imaging before diagnostic angiography is useful because it allows detailed assessment of the access route. This practice may reduce procedure time, thus resulting in fewer complications.
  • Masahito Kawabori, Toshiya Osanai, Shuho Goto, Motoyuki Iwasaki, Yoshimasa Niiya, Shoji Mabuchi, Kiyohiro Houkin
    Journal of neurosurgical sciences 62 5 612 - 614 2018年10月 [査読有り][通常論文]
  • Masahito Kawabori, Yoshimasa Niiya, Motoyuki Iwasaki, Shoji Mabuchi, Kiyohiro Houkin
    Journal of neurosurgical sciences 65 4 397 - 401 2018年09月25日 [査読有り][通常論文]
     
    INTRODUCTION: Microscope-integrated near-infrared indocyanine green (ICG) videoangiography (VA) is an effective method of intraoperative blood flow assessment and identification of plaque location during carotid endarterectomy (CEA). However, the validity of ICG-VA during CEA for patient with near occlusion has not been elucidated. PATIENTS AND METHODS: Thirty-four CEA procedures were performed between June 2009 and December 2017 for patient with near occlusion, which are more than 95% stenosis. The lesions were classified into three groups according to the diameter of distal ICA compared with ipsilateral external carotid artery (ECA), as normal diameter (>100%), moderate diameter reduction (50-100%), severe diameter reduction (<50%). 5 mg of ICG was injected intravenously before arteriotomy, and ICG-VA was taken to identify the distal end of the plaque. Depiction of the distal end and its accuracy were retrospectively reviewed. RESULTS: There were 18 cases with normal diameter group, 14 cases with moderate diameter reduction group, and 2 cases with severe diameter reduction group. All but one case in the severe diameter reduction group showed apparent distal end signal which were confirmed by arteriotomy afterward. CONCLUSIONS: The present study clearly denotes that ICG-VA can visualize the carotid plaque distal end during the CEA, even with the patient with near occlusion. However, it should be noted that there may be a difficulty in visualization of the distal plaque end for patient with severely collapsed distal ICA.
  • Oki K, Katsumata M, Izawa Y, Takahashi S, Suzuki N, Houkin K, Research Committee on, Spontaneous Occlusion of Circle of Willis, Moyamoya disease
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 27 12 3605 - 3612 2018年09月 [査読有り][通常論文]
     
    BACKGROUND: The efficacy and safety of antiplatelet drugs in the treatment of moyamoya disease remain unclear. This study reports results of a nationwide survey conducted in 2016 on the trends of antiplatelet therapy for moyamoya disease in Japan. METHODS: Data were obtained through questionnaires related to treatment policies regarding antiplatelet drugs from each specialized stroke management department of 765 hospitals in Japan. Data were also compared between experienced facilities (defined as facilities managing more than 10 cases per year) and those less experienced (not more than 10 cases per year) to determine experts' opinion. RESULTS: Of the 389 departments in 375 hospitals that responded, 330 departments provided medical care for moyamoya disease. Regarding ischemic stroke, numerous departments considered the use of antiplatelet drugs "in principle" (218 departments). After surgery for ischemic moyamoya disease, the use of antiplatelet drugs for a certain period of time was the most popular opinion (74 departments). Regarding asymptomatic moyamoya disease, majority departments reported no use of APDs "in principle" (256 departments). The experienced facilities reported "no use of antiplatelet drugs" more frequently than those less experienced for treating asymptomatic moyamoya disease. In moyamoya disease, aspirin was the most commonly used antiplatelet drugs followed by cilostazol and clopidogrel. CONCLUSIONS: This survey revealed details of treatment policies, and the selection of antiplatelet drugs widely varied across facilities. Further prospective studies are necessary to improve the current unclear situation regarding the use of antiplatelet drugs for the management of moyamoya disease.
  • Masayuki Gekka, Takeo Abumiya, Teruyuki Komatsu, Ryosuke Funaki, Kota Kurisu, Daisuke Shimbo, Masato Kawabori, Toshiya Osanai, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
    Stroke 49 8 1960 - 1968 2018年08月 [査読有り][通常論文]
     
    Background and Purpose- A hemoglobin-albumin cluster, 1 core of hemoglobin covalently bound with 3 shell albumins, designated as HemoAct was developed as a hemoglobin-based oxygen carrier. We aim to investigate neuroprotection by HemoAct in transient cerebral ischemia and elucidate its underlying mechanisms. Methods- Male rats were subjected to 2-hour transient middle cerebral artery occlusion and were then administered HemoAct transarterially at the onset of reperfusion. Neurological and pathological findings were examined after 24 hours of reperfusion to identify neuroprotection by HemoAct. Intermittent measurements of cortical blood flow and oxygen content were performed, and a histopathologic analysis was conducted on rats during the early phase of reperfusion to assess the therapeutic mechanism of HemoAct. In addition, the antioxidant effects of HemoAct were examined in hypoxia/reoxygenation-treated rat brain microvascular endothelial cells. Results- Neurological deterioration, infarct and edema development, and the activation of MMP-9 (matrix metalloprotease-9) and lipid peroxidation after 24 hours of reperfusion were significantly ameliorated by the HemoAct treatment. Reductions in blood flow and tissue partial oxygen pressure in the cortical penumbra after 6 hours of reperfusion were significantly ameliorated by the HemoAct treatment. The histopathologic analysis of the cortical penumbra revealed that HemoAct in HemoAct-treated rats showed superior microvascular perfusion with the mitigation of microvascular narrowing changes than autologous erythrocytes in nontreated rats. Although HemoAct extravasated into the ischemic core with serum protein, it did not induce an increase in serum extravasation or reactive oxygen species production in the ischemic core. In vitro experiments with rat brain microvascular endothelial cells revealed that HemoAct significantly suppressed cellular reactive oxygen species production in hypoxia/reoxygenation-treated cells, similar to albumin. Conclusions- HemoAct exerted robust neuroprotection in transient cerebral ischemia. Superior microvascular perfusion with an oxygen delivery capability and possible antioxidant effects appear to be the underlying neuroprotective mechanisms.
  • Takamiya S, Seki T, Ikeda T, Shinada SI, Hamauchi S, Terasaka S, Houkin K
    World neurosurgery 119 172 - 175 2018年08月 [査読有り][通常論文]
     
    BACKGROUND: Myelocystoceles, which are classified as closed neural tube defects, are usually covered by skin and rarely complicated by hydrocephalus. We encountered an unusual case of a terminal myelocystocele with hydrocephalus with clinical characteristics of a myelomeningocele. CASE DESCRIPTION: Severe hydrocephalus and a lumbosacral lesion were detected in the fetus of a gravid 34-year-old woman. Cesarean section was performed at 37 weeks. The neonate presented with a lumbosacral mass with a partial skin defect. As myelomeningocele was suspected, the neonate underwent surgery on the day of birth. The intraoperative findings pointed to a myelocystocele rather than a myelomeningocele. After insertion of a ventriculoperitoneal shunt, the neonate was discharged without any neurologic deficits. CONCLUSIONS: Presence of skin abnormalities, hydrocephalus, and lumbosacral mass strongly suggests a diagnosis of myelomeningocele. However, such cases should be differentiated from myelocystocele, especially when associated with severe hydrocephalus.
  • Uchino H, Ito M, Kazumata K, Hama Y, Hamauchi S, Terasaka S, Sasaki H, Houkin K
    BMC medical genomics 11 1 72 - 72 2018年08月 [査読有り][通常論文]
     
    BACKGROUND: Moyamoya disease (MMD) is characterized by progressive stenosis of intracranial arteries in the circle of Willis with unknown etiology even after the identification of a Moyamoya susceptible gene, RNF213. Recently, differences in epigenetic regulations have been investigated by a case-control study in MMD. Here, we employed a disease discordant monozygotic twin-based study design to unmask potential confounders. METHODS: Circulating genome-wide microRNA (miRNome) profiling was performed in MMD-discordant monozygotic twins, non-twin-MMD patients, and non-MMD healthy volunteers by microarray followed by qPCRvalidation, using blood samples. Differential plasma-microRNAs were further quantified in endothelial cells differentiated from iPS cell lines (iPSECs) derived from another independent non-twin cohort. Lastly, their target gene expression in the iPSECs was analyzed. RESULTS: Microarray detected 309 plasma-microRNAs in MMD-discordant monozygotic twins that were also detected in the non-twin cohort. Principal component analysis of the plasma-microRNA expression level demonstrated distinct 2 groups separated by MMD and healthy control in the twin- and non-twin cohorts. Of these, differential upregulations of hsa-miR-6722-3p/- 328-3p were validated in the plasma of MMD (absolute log2 expression fold change (logFC) > 0.26 for the twin cohort; absolute logFC > 0.26, p < 0.05, and q < 0.15 for the non-twin cohort). In MMD derived iPSECs, hsa-miR-6722-3p/- 328-3p showed a trend of up-regulation with a 3.0- or higher expression fold change. Bioinformatics analysis revealed that 41 target genes of miR-6722-3p/- 328-3p were significantly down-regulated in MMD derived iPSECs and were involved in STAT3, IGF-1-, and PTEN-signaling, suggesting a potential microRNA-gene expression interaction between circulating plasma and endothelial cells. CONCLUSIONS: Our MMD-discordant monozygotic twin-based study confirmed a novel circulating microRNA signature in MMD as a potential diagnostic biomarker minimally confounded by genetic heterogeneity. The novel circulating microRNA signature can contribute for the future functional microRNA analysis to find new diagnostic and therapeutic target of MMD.
  • Kazuyoshi Yamazaki, Toshitaka Seki, Masahito Kawabori, Syuji Hamauchi, Izumi Koyanagi, Kiyohiro Houkin
    JOURNAL OF NEUROTRAUMA 35 16 A168 - A168 2018年08月 [査読有り][通常論文]
  • Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Michinari Okamoto, Hiroyuki Kobayashi, Kenji Hirata, Yoshitaka Oda, Shinya Tanaka, Shunsuke Terasaka, Kiyohiro Houkin
    Journal of neurosurgical sciences 64 6 502 - 508 2018年07月09日 [査読有り][通常論文]
     
    BACKGROUND: Although newly diagnosed high-grade glioma patients in Japan can receive bevacizumab (BEV) as first-line chemotherapy, randomized clinical trials have not shown a survival benefit for BEV for these patients. In this study, we investigated whether selective add-on BEV for patients with newly diagnosed glioblastoma (GBM) and anaplastic astrocytoma (AA) improves prognosis, in cases where tumors were continuously growing during radiotherapy concomitant with temozolomide (TMZ). METHODS: We conducted a retrospective survey of the overall survival (OS) of patients with GBM/AAs who were treated in our institution between 2006 and 2016. Patients whose tumors were continuously growing regardless of radiotherapy were categorized as the "progressive" group; remaining patients were categorized as the "non-progressive" group. Since 2013, patients in the "progressive" group received add-on BEV therapy with the Stupp regimen during or just after radiotherapy. RESULTS: Of 151 GBM/AA patients, 34 (22.5%) were categorized in the "progressive" group. Median OSs of the "progressive" and "non-progressive" groups were 13.2 months and 25.3 months, respectively (P < 0.001). Twelve patients in the "progressive" group received add-on BEV therapy, and their median OS was 20.2 months; whereas for the remaining 22 patients in the "progressive" group who were treated before the BEV era, their median OS was 10.5 months. In the "progressive" group, add-on BEV significantly extended OS (P = 0.018) and was the lone clinical factor of better prognosis. CONCLUSIONS: We found that, for patients with GBM/AAs whose tumors were continuously growing during radiotherapy, add-on BEV treatment resulted in survival benefits.
  • Rintaro Yokoyama, Takeshi Mikami, Ryo Ukai, Katsuya Komatsu, Yusuke Kimura, Hime Suzuki, Toshimi Honma, Toru Hirano, Tamotsu Saito, Ken Yamashita, Takatoshi Yotsuyanagi, Kiyohiro Houkin, Nobuhiro Mikuni
    WORLD NEUROSURGERY 115 247 - 253 2018年07月 [査読有り][通常論文]
     
    Extracranial-to-intracranial (EC-IC) bypass surgery may be necessary in patients with moyamoya disease and other ischemic conditions. However, there is a potential risk of wound-related complications in some cases. In this study, we report our approach to the prevention of wound-related complications in EC-IC bypass. Technical considerations and pitfalls of surgery are also discussed. This study included 89 patients with ischemic-onset moyamoya disease and atherosclerotic disease who underwent 108 superficial temporal artery (STA) etoemiddle cerebral artery bypass procedures. Our study emphasized 3 major features. First, 3-dimensional simulation imaging was used to confirm STA anatomy. Second, the STA was meticulously dissected on the epigaleal layer to protect the galeal layer. Third, scalp skin ischemia was taken into consideration at each step until skin closure. There was no neurologic morbidity or mortality in this series. There were 2 cases of major wound-related complications requiring plastic surgical intervention, and 4 cases of minor complications that were treated conservatively. In major complication cases, the scalp defect was treated with pedicle flap reconstruction. In EC-IC bypass surgery, interdisciplinary management involving neurosurgery, plastic surgery, and radiology should reduce wound-related complications and achieve safe surgery.
  • Yasuhiro Takahashi, Takeshi Mikami, Hime Suzuki, Katsuya Komatsu, Daisuke Yamamoto, Shun Shimohama, Kiyohiro Houkin, Shintaro Sugita, Tadashi Hasegawa, Nobuhiro Mikuni
    Journal of Clinical Neuroscience 53 250 - 253 2018年07月01日 [査読有り][通常論文]
     
    We report a case of moyamoya disease (MMD), which developed after non-herpetic acute limbic encephalitis (NHALE) associated with anti-leucine-rich glioma-inactivated 1 (LGI1) antibody. The patient's mother had a history of MMD. No vascular lesions were identified at the time of the NHALE. Nine years later, the patient visited our hospital due to memory disturbances and repeated transient ischemic attacks affecting the right limb. Diffusion-weighted magnetic resonance imaging revealed scattered areas of signal hyperintensity, and the patient was ultimately diagnosed with MMD based on angiography. Revascularization surgery was performed on the left side, where cerebral blood flow was impaired on 123I-N-isopropyl-p-iodoamphetamine single photon emission computed tomography. Postoperatively, the patient was discharged with a normal neurological examination. NHALE associated with LGI1 antibodies is an autoimmune disease. Although autoimmune disease is the most frequent finding other than atherosclerosis in quasi-MMD, this is the first report of NHALE associated with anti-LGI1 antibodies mimicking quasi-MMD. Inflammation and angiogenesis may contribute to the development of MMD, in addition to genetic background.
  • Toshitaka Seki, Kazutoshi Hida, Shunsuke Yano, Kiyohiro Houkin
    Asian Spine Journal 12 23 551 - 555 2018年06月01日 [査読有り][通常論文]
     
    Study Design: A retrospective cohort study. Purpose: To examine the validity of prophylactic surgery for children with tethered cord syndrome (TCS). Overview of Literature: Prophylactic surgery for pediatric patients with TCS remains controversial. Methods: We retrospectively analyzed the surgical outcomes of 14 children (nine boys and five girls) with asymptomatic TCS who were surgically treated at Hokkaido University Hospital between 1989 and 2015. Results: The median age at the time of initial surgery for asymptomatic TCS was 28.6 months (range, 0-66 months). The median final follow-up period was 142 months (range, 7-232 months). Of the 14 children with asymptomatic TCS, 12 had lumbosacral lipoma and two had meningocele. According to the classification of spinal lipoma, two children had dorsal type, four had caudal type, two had transitional type, and four had filar type. There were no children with lipomyelomeningocele. All children were free of neurological symptoms until 94 months after the initial surgery. Subsequently, one child exhibited delayed neurological deficits and underwent a second surgery because of motor and sensory disturbances slight sensory disturbance was noted at the final follow-up examination. Another child later showed bowel and bladder dysfunction. However, a second surgery was not performed for this child because his motor and sensory functions were normal hence, we chose to avoid nerve injury in the case of dissecting adhesion. Conclusions: All 14 children with asymptomatic TCS were free of neurological symptoms until 94 months after the initial surgery. However, two children exhibited delayed neurological deficits at 94 months and 177 months. We believe that prophylactic surgery for asymptomatic TCS is effective for a certain period. However, because the natural history of TCS is poorly understood, strict follow-up after surgery is necessary.
  • Toshiya Osanai, Kiyohiro Houkin, Shinichiro Uchiyama, Kazuo Minematsu, Akihiko Taguchi, Shunsuke Terasaka
    International journal of stroke : official journal of the International Stroke Society 13 4 444 - 448 2018年06月 [査読有り][通常論文]
     
    Rationale MultiStem® (HLM051) is one of the promising allogenic cell products for acute ischemic stroke with strong evidence. A previous phase 2 randomized, double-blind, placebo-controlled, multicenter dose-escalation trial showed the safety of MultiStem® for acute ischemic stroke, with a time window beyond that of rt-PA and endovascular thrombectomy. We aim to obtain stronger evidence and to show the efficacy of the MultiStem® for treatment of ischemic stroke. Sample size Estimated sample size is 220 (110 patients per group), which has 90% power at 5% significance level. Methods and design TREASURE is a randomized, double-blind, placebo-controlled, multicenter phase 2/3 trial. The trial will be done at 31 medical centers in Japan. Patients with acute ischemic stroke including motor or speech deficit defined by a National Institution of Health Stroke Scale (NIHSS) score of 8-20 at baseline will be randomized 1:1 to receive a single intravenous infusion of MultiStem® or placebo within 18-36 h of stroke onset. Study outcomes Primary outcome in this study is the proportion of patients with an excellent outcome at day 90 defined by the functional assessment. Trial registration ClinicalTrials.gov (NCT02961504). Conclusion The TREASURE trial will provide a novel treatment option and expand the therapeutic window for patients with stroke if the results are positive.
  • Chengbo Tan, Songji Zhao, Kei Higashikawa, Zifeng Wang, Masahito Kawabori, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Naoyuki Ukon, Hironobu Yasui, Nagara Tamaki, Yuji Kuge, Hideo Shichinohe, Kiyohiro Houkin
    EJNMMI research 8 1 35 - 35 2018年05月02日 [査読有り][通常論文]
     
    BACKGROUND: The potential application of bone marrow stromal cell (BMSC) therapy in stroke has been anticipated due to its immunomodulatory effects. Recently, positron emission tomography (PET) with [18F]DPA-714, a translocator protein (TSPO) ligand, has become available for use as a neural inflammatory indicator. We aimed to evaluate the effects of BMSC administration after transient middle cerebral artery occlusion (MCAO) using [18F]DPA-714 PET. The BMSCs or vehicle were administered intravenously to rat MCAO models at 3 h after the insult. Neurological deficits, body weight, infarct volume, and histology were analyzed. [18F]DPA-714 PET was performed 3 and 10 days after MCAO. RESULTS: Rats had severe neurological deficits and body weight loss after MCAO. Cell administration ameliorated these effects as well as the infarct volume. Although weight loss occurred in the spleen and thymus, cell administration suppressed it. In both vehicle and BMSC groups, [18F]DPA-714 PET showed a high standardized uptake value (SUV) around the ischemic area 3 days after MCAO. Although SUV was increased further 10 days after MCAO in both groups, the increase was inhibited in the BMSC group, significantly. Histological analysis showed that an inflammatory reaction occurred in the lymphoid organs and brain after MCAO, which was suppressed in the BMSC group. CONCLUSIONS: The present results suggest that BMSC therapy could be effective in ischemic stroke due to modulation of systemic inflammatory responses. The [18F]DPA-714 PET/CT system can accurately demonstrate brain inflammation and evaluate the BMSC therapeutic effect in an imaging context. It has great potential for clinical application.
  • Masahito Kawabori, Yoshimasa Niiya, Motoyuki Iwasaki, Shoji Mabuchi, Hiroyuki Ozaki, Koji Matsubara, Kiyohiro Houkin
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 27 5 1338 - 1342 2018年05月 [査読有り][通常論文]
     
    BACKGROUND: Direct oral coagulants (DOAC) have been shown to decrease the frequency of intracerebral hemorrhage (ICH) compared with warfarin. However, the precise characteristics, such as the size and locations of the hemorrhage, and outcome and onset time of ICH in patient taking DOAC are not fully elucidated. METHODS: We retrospectively analyzed the characteristics of symptomatic patients with ICH taking either DOAC or warfarin between January 2012 and December 2015. RESULTS: Out of 400 consecutive patients with ICH, 15 patients were DOAC-ICH and 24 patients were warfarin-ICH. DOAC-ICH was observed in 6 patients with 10 mg of rivaroxaban, 5 patients with 15 mg of rivaroxaban, and 1 patient with 10 mg of apixaban, 5 mg of apixaban, 30 mg of edoxaban, and 60 mg of edoxaban. Prothrombin time was well controlled in most of the warfarin-ICH patients (83.3%). The locations of ICH were similar in both groups; however, median ICH volume was significantly smaller in DOAC-ICH patients than in warfarin-ICH patients (P < .01) and ICH around basal ganglia seemed to show great difference between the groups. DOAC-ICH patients showed better neurological outcome at the time of discharge than warfarin patients (P < .01), and the ratio of good prognosis was significantly higher in the DOAC-ICH patients than in the warfarin-ICH patients (P < .01). The onset of warfarin-ICH was frequently observed in the morning and evening, whereas DOAC-ICH did not show any specific onset time. CONCLUSIONS: Patients with DOAC-ICH showed smaller ICH volume and better clinical outcomes than patients with warfarin-ICH, and DOAC-ICH did not show any specific onset peak.
  • Kiyohiro Houkin, Hideo Shichinohe, Koji Abe, Teruyo Arato, Mari Dezawa, Osamu Honmou, Nobutaka Horie, Yasuo Katayama, Kohsuke Kudo, Satoshi Kuroda, Tomohiro Matsuyama, Ichiro Miyai, Izumi Nagata, Kuniyasu Niizuma, Ken Sakushima, Masanori Sasaki, Norihiro Sato, Kenji Sawanobori, Satoshi Suda, Akihiko Taguchi, Teiji Tominaga, Haruko Yamamoto, Toru Yamashita, Toshiki Yoshimine
    Stroke 49 4 e145-e152 - e152 2018年04月 [査読有り][通常論文]
  • Takeshi Funaki, Jun C Takahashi, Kiyohiro Houkin, Satoshi Kuroda, Shigekazu Takeuchi, Miki Fujimura, Yasutake Tomata, Susumu Miyamoto
    Journal of neurosurgery 128 3 777 - 784 2018年03月 [査読有り][通常論文]
     
    OBJECTIVE In this paper, the authors set out to identify the angiographic features of moyamoya disease with posterior hemorrhage, which is a strong predictor of rebleeding. METHODS This cross-sectional study used the data set of the Japan Adult Moyamoya Trial (clinical trial registration no.: C000000166 [ www.umin.ac.jp/ctr/index.htm ]). The panel designed the ancillary measurement of angiography at onset, classifying the collateral vessels into 3 subtypes: lenticulostriate anastomosis, thalamic anastomosis, and choroidal anastomosis. The association between each collateral and the hemorrhage site (anterior vs posterior) was assessed in the hemorrhagic hemisphere by using multivariate adjustment for potential confounders, including age, sex, and involvement of the posterior cerebral artery (PCA). The association was confirmed through topographical analysis of bleeding points. RESULTS Among the 80 participants, 75 hemorrhagic hemispheres of 75 patients were analyzed. Lenticulostriate anastomosis was detected in 21 (28.0%) hemorrhagic hemispheres, thalamic anastomosis in 22 (29.3%), and choroidal anastomosis in 35 (46.7%). Choroidal anastomosis was a factor associated with posterior hemorrhage (OR 2.77 [95% CI 1.08-7.10], p = 0.034) and remained statistically significant after the multivariate adjustment (OR 2.66 [95% CI 1.00-7.07], p = 0.049). PCA involvement was also associated with posterior hemorrhage in both univariate and multivariate analyses. Topographical analysis revealed good correspondence between bleeding points associated with positive choroidal anastomosis and the anatomical distribution of the choroidal arteries, including the thalamus and the wall of the atrium. CONCLUSIONS Choroidal anastomosis and PCA involvement are characteristic of posterior hemorrhage in moyamoya disease. Choroidal anastomosis might be considered a potential source of posterior hemorrhage at high risk of rebleeding.
  • Toshiya Osanai, Kiyohiro Houkin
    Case Reports in Neurology 10 1 112 - 117 2018年02月14日 [査読有り][通常論文]
     
    Sinus pericranii is a rare vascular anomaly, and most cases occur in children and develop at the midline. In previous reports of sinus pericranii, T2 hyperintensity lesion has not been regarded as a common sequela. We report an extremely rare case of orbital sinus pericranii with associated T2 hyperintensity lesion. A 50-year-old man was admitted to our hospital with a history of right upper eyelid swelling that had been present for several years. Computed tomography, magnetic resonance imaging, and digital subtraction angiography demonstrated a connection between the lesion and normal cerebral venous system. Thus, we diagnosed the lesion as a sinus pericranii despite its atypical features. We elected to observe the patient, and the lesion had remained the same size without any adverse events, such as hemorrhage, occurring throughout the 5-year follow-up. An atypical sinus pericranii should be considered in patients with a soft compressible swelling on the head, even if the lesion is located off the midline.
  • Masayuki Gekka, Naoki Nakayama, Haruto Uchino, Kiyohiro Houkin
    Acta Neurochirurgica 160 2 269 - 276 2018年02月01日 [査読有り][通常論文]
     
    Background: Indocyanine green video-angiography (ICG-V) is commonly used for intraoperative confirmation of aneurysm obliteration following clipping. However, direct puncture of the aneurysm wall occasionally results in blood leakage in patients for whom ICG-V has indicated complete closure. Therefore, the present study aimed to determine the reliability of ICG-V for confirming complete aneurysm closure, and to elucidate the factors underlying aneurysm obliteration and the occurrence of false-negative ICG-V findings. Methods: Between June 2012 and June 2016, 89 patients (107 aneurysms total) undergoing aneurysm clipping were examined using ICG-V to confirm aneurysm closure. In ICG-V-negative cases, further confirmation of complete aneurysm closure was obtained via direct puncture of the aneurysm wall, except in cases where this procedure was deemed unsafe. To elucidate the possible causes of ICG-V inaccuracies, positive, negative, and false-negative ICG-V findings were compared in terms of aneurysm location (maximum height and length), neck width (parallel and orthogonal directions to the branching vessels), wall thickness around the neck, bifurcation angle, and direction of the clipping closure line. Statistical analyses were performed using the Welsh’s t test and Chi-square test. Results: Intraoperative ICG-V detected seven cases of incomplete aneurysm closure (6.5%), defined as positive ICG-V findings. Following direct aneurysm wall puncture, nine patients (8.4%) exhibited false-negative ICG-V findings. A Chi-square test revealed that false-negative ICG-V findings were significantly influenced by the presence of heterogeneous arteriosclerosis, and wall thickening at the clipping site, which were subjectively defined by the surgeon and confirmed by an independent observer, depending on the wall color and hardness, respectively. Conclusions: Although ICG-V is useful for intraoperative confirmation of aneurysm obliteration, our findings further highlight the risk of false-negative ICG-V findings. Acknowledgement of risk factors is crucial for efficient detection of false-negative ICG-V findings.
  • Michiyuki Miyamoto, Kentaro Nakamura, Hideo Shichinohe, Tomohiro Yamauchi, Masaki Ito, Hisayasu Saito, Masahito Kawabori, Toshiya Osanai, Tasuku Sasaki, Kiyohiro Houkin, Satoshi Kuroda
    Stem cells international 2018 4829534 - 4829534 2018年 [査読有り][通常論文]
     
    Bone marrow stromal cell (BMSC) transplantation has the therapeutic potential for ischemic stroke. However, it is unclear which delivery routes would yield both safety and maximal therapeutic benefits. We assessed whether a novel recombinant peptide (RCP) sponge, that resembles human collagen, could act as a less invasive and beneficial scaffold in cell therapy for ischemic stroke. BMSCs from green fluorescent protein-transgenic rats were cultured and Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAo). A BMSC-RCP sponge construct was transplanted onto the ipsilateral intact neocortex 7 days after MCAo. A BMSC suspension or vehicle was transplanted into the ipsilateral striatum. Rat motor function was serially evaluated and histological analysis was performed 5 weeks after transplantation. The results showed that BMSCs could proliferate well in the RCP sponge and the BMSC-RCP sponge significantly promoted functional recovery, compared with the vehicle group. Histological analysis revealed that the RCP sponge provoked few inflammatory reactions in the host brain. Moreover, some BMSCs migrated to the peri-infarct area and differentiated into neurons in the BMSC-RCP sponge group. These findings suggest that the RCP sponge may be a promising candidate for animal protein-free scaffolds in cell therapy for ischemic stroke in humans.
  • 寳金清博, 寳金清博, 寳金清博, 寳金清博, 寳金清博
    国際臨床医学会雑誌(Web) 2 1 2018年
  • 東海林菊太郎, 長内俊也, 藤間憲幸, 牛越聡, 寳金清博
    脳血管内治療(Web) 3 Supplement 2018年
  • Kensuke Fujiwara, Toshiya Osanai, Eiichi Kobayashi, Takumi Tanikawa, Ken Kazumata, Kikutaro Tokairin, Kiyohiro Houkin, Katsuhiko Ogasawara
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 27 1 177 - 184 2018年01月 [査読有り][通常論文]
     
    BACKGROUND: Both the accessibility and availability of stroke specialists are major determinants of patient outcomes following acute ischemic stroke (AIS). The purpose of this study was to implement novel metrics to assess the accessibility of tertiary stroke centers as well as to evaluate regional disparities in stroke specialists. METHODS: Using network analysis in a geographic information system, we calculated areas within 30- and 60-minute travel times to facilities providing intravenous recombinant tissue-type plasminogen activator and mechanical thrombectomy. We further evaluated the accessibility for the proportion of the population aged 65 years or older that resided outside of these areas. Uniformity in the geographical distribution of stroke specialists was then evaluated using optimal statistical analysis. RESULTS: Accessibility varied widely from region to region, with low accessibility being concentrated in rural areas with low population density. Accessibility to facilities providing mechanical thrombectomy was especially low, and 17.8% of elderly individuals lived ≥60 minutes from treatment facilities. In addition, the distribution of stroke specialists was uneven compared with the distribution of hospital beds and full-time medical doctors. CONCLUSION: The results of this study revealed regional disparities in the spatial accessibility to treatment facilities, as well as in the distribution of stroke specialists in Hokkaido. These findings provide useful information that could be employed to appropriately allocate resources toward the formation of a medical supply system for patients with AIS.
  • Soichiro Takamiya, Toshitaka Seki, Kazuyoshi Yamazaki, Toru Sasamori, Kiyohiro Houkin
    World Neurosurgery 109 18 - 23 2018年01月01日 [査読有り][通常論文]
     
    Background Spinal arachnoid cysts (SACs) are filled with cerebrospinal fluid, and they include the arachnoid membrane, making it difficult to distinguish the walls of the cyst from the arachnoid membrane and excise the cyst as a lump. Here we report a technique for the intraoperative visualization of SACs, involving the use of pyoktanin blue. Methods Four patients with spinal intradural arachnoid cysts underwent total excision of the cysts between October 2016 and April 2017. In 1 case, magnetic resonance imaging revealed the cyst clearly, but in the other cases, the cysts were unclear. All cysts were injected with 1% pyoktanin blue (Wako Pure Chemical Industries, Osaka, Japan) diluted 500 times with physiological saline before excision. When it was difficult to distinguish the cyst from the normal arachnoid membrane, 1% pyoktanin blue diluted 1000 times with physiological saline was injected into both the cyst and the subarachnoid space, and the spread of the stain was observed. Results The cysts were better visualized after pyoktanin blue injection than before injection. When it was difficult to distinguish the cyst from the normal arachnoid space, pyoktanin blue injection was useful for judging the cyst space. There were no perioperative complications, and the patients’ symptoms improved partially or completely after treatment. Conclusions Our technique of pyoktanin blue injection into SACs could make their excision easy and safe.
  • Daina Kashiwazaki, Naoki Akioka, Naoya Kuwayama, Kiyohiro Houkin, Marcus Czabanka, Peter Vajkoczy, Satoshi Kuroda
    NEUROSURGERY 81 6 986 - 991 2017年12月 [査読有り][通常論文]
     
    BACKGROUND: The grading system for moyamoya disease is not established. OBJECTIVE: To assess the usefulness of a recently proposed grading system for stratifying the clinical severity and predicting postoperative morbidity in adult moyamoya disease. METHODS: We investigated 176 hemispheres from 89 adult patients who were diagnosed with moyamoya disease in Japan. Their data were analyzed using the Berlin grading system with minor modifications. After summarizing the numerical values for digital subtraction angiography (1-3 points), magnetic resonance imaging (0-1 points), and single-photon emission computed tomography (0-2 points), 3 grades of moyamoya disease were defined: mild (grade I) = 1 to 2 points, moderate (grade II) = 3 to 4 points, and severe (grade III) = 5 to 6 points. In total, 82 of 161 hemispheres underwent superficial temporal artery to middle cerebral artery anastomosis and indirect synangiosis. Postoperative neurological morbidity was included within 30 d after surgery. RESULTS: Preoperative examinations categorized 87 hemispheres as grade I, 39 as grade II, and 50 as grade III. There was a significant correlation between the Berlin grading system and clinical severity (P<.001). Perioperative complications occurred in 12 of 82 (14.6%) hemispheres, including transient ischemic attack in 3 hemispheres, ischemic stroke in 4 hemispheres, symptomatic hyperperfusion in 4 hemispheres, and intracerebral hemorrhage in 1 hemisphere. The Berlin grading system was related to their occurrence (P<.001). CONCLUSION: The Berlin grading system facilitates the stratification of clinical severity and predicting postoperative neurological morbidity in adult moyamoya disease, thereby suggesting its general usage in clinical practice.
  • Daisuke Shimbo, Takeo Abumiya, Kota Kurisu, Toshiya Osanai, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Hideki Nakamura, Hiroshi Shimuzu, Kiyohiro Houkin
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 26 12 2994 - 3003 2017年12月 [査読有り][通常論文]
     
    Background: The development of cerebral infarction after transient ischemia is attributed to postischemic delayed hypoperfusion in the microvascular region. In the present study, we assessed the microvascular perfusion capacity of infused liposome-encapsulated hemoglobin (LEH) in a therapeutic approach for transient middle cerebral artery occlusion (tMCAO). Methods: Two-hour middle cerebral artery occlusion rats were immediately subjected to intra-arterial infusion of LEH (LEH group) or saline (vehicle group) or no treatment (control group), and then to recanalization. Neurological findings, infarct and edema progression, microvascular endothelial dysfunction, and inflammatory reactions were compared between the 3 groups after 24 hours of reperfusion. Microvascular perfusion in the early phase of reperfusion was evaluated by hemoglobin immunohistochemistry and transmission electron microscopy. Results: The LEH group achieved significantly better results in all items evaluated than the other groups. Hemoglobin immunohistochemistry revealed that the number of hemoglobin-positive microvessels was significantly greater in the LEH group than in the other groups (P < .01), with microvascular perfusion being more likely in narrow microvessels (<= 5 mu m in diameter). An electron microscopic examination revealed that microvessels in the control group were compressed and narrowed by swollen astrocyte end-feet, whereas those in the LEH group had a less deformed appearance and contained LEH particles and erythrocytes. Conclusion: The results of the present study demonstrated that the infusion of LEH reduced infarctions after tMCAO with more hemoglobin-positive and less deformed microvessels at the early phase of reperfusion, suggesting that the superiority of the microvascular perfusion of LEH mediates its neuroprotective effects.
  • Haruto Uchino, Ken Kazumata, Masaki Ito, Naoki Nakayama, Kiyohiro Houkin
    JOURNAL OF NEUROSURGERY-PEDIATRICS 20 5 485 - 488 2017年11月 [査読有り][通常論文]
     
    OBJECTIVE A specific population of young patients with moyamoya disease (MMD) persistently experience physical symptoms not attributable to focal ischemia. These symptoms, highly suggestive of orthostatic intolerance (also termed "orthostatic dysregulation"), were investigated and reported as potential determinants of quality of life in young MMD patients. METHODS Forty-six patients (6-30 years of age) were selected from a group of 122 patients who were diagnosed with MMD before 18 years of age. The authors administered a structured questionnaire consisting of 11 items based on screening checklists published in the Japanese clinical guidelines for juvenile orthostatic dysregulation in young patients. The results were tabulated, and correlations with clinical data were explored. RESULTS Thirty-seven (80%) patients (mean age 15.9 years) responded to the questionnaire. Frequent headache, vertigo/dizziness on standing, fatigue, difficulty with getting out of bed, and motion sickness were the top 5 symptoms, resulting in 57% of patients being unable to attend school. Forty-three percent of the patients demonstrated multiple symptoms suggestive of orthostatic intolerance, even as long as 5 years after revascularization surgery. The number of symptoms was inversely associated with the number of years after surgery (p = 0.028). The number of symptoms was not associated with a history of surgery, clinical presentations, vascular involvement, cerebral perfusion, brain lesions, or history of transient ischemic attacks. CONCLUSIONS The present study provided novel insight into the symptomatology of young patients with MMD. Failure to notice nonfocal physical symptoms can significantly impair quality of life in young patients with MMD even years after successful revascularization surgery. These symptoms may serve as independent clinical markers used to assess disease outcome, although the underlying mechanisms of this disease are, as of yet, unclear.
  • Yukitomo Ishi, Kanako C. Hatanaka, Shigeru Yamaguchi, Hiromi Fujita, Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
    BRAIN TUMOR PATHOLOGY 34 4 165 - 171 2017年10月 [査読有り][通常論文]
     
    A 5-year-old boy underwent biopsy of an intra-axial calcified tumor in the hypothalamus, which was incidentally found. Based on the presence of ganglion-like cells combined with glial cell element, the pathological diagnosis was ganglioglioma. Because the tumor grew gradually in size over the next 2 years, he underwent chemotherapy with temozolomide. However, at 8 years of age, the boy developed hydrocephalus and the cystic lesion had re-grown. Endoscopic cyst fenestration and tumor biopsy was performed, and pathological diagnosis was tentatively oligodendroglioma based on the presence of tumor cells with a perinuclear halo. At 10 years of age, hydrocephalus recurred and the cystic lesion had re-grown. A second round of endoscopic cyst fenestration and tumor biopsy led to a pathological diagnosis of pilocytic astrocytoma due to a biphasic appearance with areas of dense tumor cells and microcystic areas, tumor cells with eosinophilic processes, and the presence of an eosinophilic granular body. Genetic analysis of the first biopsy successfully identified the BRAF V600E mutation. Because pathological diagnosis of diencephalic low-grade glioma harboring BRAF V600E would be sometimes difficult due to pathological variations, pathological diagnosis should be performed under the consideration of molecular diagnosis of BRAF V600E for optimal diagnosis and treatment.
  • Iwasaki M, Akiyama M, Koyanagi I, Niiya Y, Ihara T, Houkin K
    NMC case report journal 4 4 121 - 125 2017年10月 [査読有り][通常論文]
     
    We present a case of double-crushed L5 nerve root symptoms caused by inside and outside of the spinal canal with spur formation of the lumbosacral transitional vertebra (LSTV). A 78-year-old man presented with 7-year history of moderate paresis of his toe and left leg pain when walking. Magnetic resonance imaging (MRI) revealed spinal stenosis at the L3/4 and 4/5 spinal levels and he underwent wide fenestration of both levels. Leg pain disappeared and 6-min walk distance (6MWD) improved after surgery, however, the numbness in his toes increased and 6MWD decreased 9 months after surgery. Repeated MR and 3D multiplanar reconstructed computed tomography (CT) images showed extraforaminal impingement of the L5 root by bony spur of the left LSTV. He underwent second decompression surgery of the L5/S via the left sided Wiltse approach, resulting in the improvement of his symptoms. The impingement of L5 spinal nerve root between the transverse process of the fifth lumbar vertebra and the sacral ala is a rare entity of the pathology called "far-out syndrome (FOS)". Especially, the bony spur formation secondary to the anomalous articulation of the LSTV (LSPA) has not been reported. These articulations could be due to severe disc degeneration, following closer distance and contact between the transverse process and the sacral ala. To our knowledge, this is the first report describing a case with this pathology and may be considered in cases of failed back surgery syndromes (FBSS) of the L5 root symptoms.
  • Hideo Shichinohe, Masahito Kawabori, Hiroaki Iijima, Tuyoshi Teramoto, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Shunsuke Terasaka, Teruyo Arato, Kiyohiro Houkin
    BMC NEUROLOGY 17 1 179 - 179 2017年09月 [査読有り][通常論文]
     
    Background: Stroke is a leading cause of death and disability, and despite intensive research, few treatment options exist. However, a recent breakthrough in cell therapy is expected to reverse the neurological sequelae of stroke. Although some pioneer studies on the use of cell therapy for treating stroke have been reported, certain problems remain unsolved. Recent studies have demonstrated that bone marrow stromal cells (BMSCs) have therapeutic potential against stroke. We investigated the use of autologous BMSC transplantation as a next-generation cell therapy for treating stroke. In this article, we introduce the protocol of a new clinical trial, the Research on Advanced Intervention using Novel Bone marrOW stem cell (RAINBOW). Methods/design: RAINBOW is a phase 1, open-label, uncontrolled, dose-response study, with the primary aim to determine the safety of the autologous BMSC product HUNS001-01 when administered to patients with acute ischemic stroke. Estimated enrollment is 6-10 patients suffering from moderate to severe neurological deficits. Approximately 50 mL of the bone marrow is extracted from the iliac bone of each patient 15 days or later from the onset. BMSCs are cultured with allogeneic human platelet lysate (PL) as a substitute for fetal calf serum and are labeled with superparamagnetic iron oxide for cell tracking using magnetic resonance imaging (MRI). HUNS00101 is stereotactically administered around the area of infarction in the subacute phase. Each patient will be administered a dose of 20 or 50 million cells. Neurological scoring, MRI for cell tracking, F-18-fuorodeoxyglucose positron emission tomography, and I-123-Iomazenil singlephoton emission computed tomography will be performed for 1 year after the administration. Discussion: This is a first-in-human trial for HUNS001-01 to the patients with acute ischemic stroke. We expect that intraparenchymal injection can be a more favorable method for cell delivery to the lesion and improvement of the motor function than intravenous infusion. Moreover, it is expected that the bio-imaging techniques can clarify the therapeutic mechanisms.
  • Toshiya Osanai, Kazutoshi Hida, Takeshi Asano, Toshitaka Seki, Toru Sasamori, Kiyohiro Houkin
    WORLD NEUROSURGERY 104 841 - 847 2017年08月 [査読有り][通常論文]
     
    BACKGROUND: The goal of treatment for spinal arteriovenous lesions is to completely obliterate the shunt. In our institution, intraoperative digital subtraction angiography and intraarterial injection of contrast agent have been used to accurately identify the site of arteriovenous shunts. We describe the intraoperative digital subtraction angiography and intraarterial dye injection procedures and how they may improve surgical outcomes. METHODS: We retrospectively investigated 22 patients with intradural arteriovenous lesions (n = 19) or spinal dural arteriovenous fistulas (n = 3). A microcatheter was used during the procedures to avoid catheter migration. RESULTS: There were 29 procedures performed. To support the surgical procedures, indigo carmine was used 17 times and indocyanine green was used 12 times. There were no complications associated with these procedures. The indocyanine green procedure required a lower concentration of dye in the artery than in the vein to clarify the shunt point and visualized complex lesions more clearly. These methods allowed surgeons to orientate the complex vessel structure. CONCLUSIONS: Intraoperative digital subtraction angiography and intraarterial dye injection are useful tools for management of spinal arteriovenous lesions.
  • Tomoyoshi Kuribara, Takeshi Mikami, Katsuya Komatsu, Hime Suzuki, Hirofumi Ohnishi, Kiyohiro Houkin, Nobuhiro Mikuni
    BMC NEUROLOGY 17 1 149 - 149 2017年08月 [査読有り][通常論文]
     
    Background: Enlarged perivascular spaces (EPVS) are often observed with magnetic resonance imaging in patients with small vessel disease. However, the risk factors, radiological features, and clinical relevance of EPVS in patients with moyamoya disease are poorly understood. The purpose of this study was to evaluate EPVS, the risk factors of many EPVS, and the pathophysiology of EPVS in adult patients with moyamoya disease. Methods: One hundred cerebral hemispheres of 50 adult patients with moyamoya disease were examined. The control group consisted of 50 age/sex-matched patients without ischemic disease. The numbers of EPVS at the level of the centrum semiovale per hemisphere were compared between the moyamoya disease and control groups. In each hemisphere, the total numbers of EPVS were categorized into five grades (0-4), and the clinical and radiological characteristics of the predictive factors in patients in the high EPVS grade group (EPVS grade = 4) were assessed. Results: The EPVS counts and grades were significantly higher in the moyamoya disease group. Analyses of the background characteristics of the patients with moyamoya disease revealed that significantly higher prevalence of high EPVS grades were associated with the female sex, hypertension, high magnetic resonance angiography scores, high numbers of flow voids in the basal ganglia, high brain atrophy scores, ivy signs, and white matter lesions. A logistic multivariate analysis of the patients with high EPVS grades revealed significant associations with the female sex, hypertension, and flow voids in the basal ganglia. Conclusions: Increased EPVS were confirmed in adult patients with moyamoya disease, and the associated clinical and radiological factors were identified. The presence of hypertension, the female sex, and flow voids in the basal ganglia were important for predicting high EPVS grades in patients with moyamoya disease. Reductions in arterial pulsations with steno-occlusive changes can inhibit the flow of interstitial fluid, which can increase the number of EPVS in patients with moyamoya disease. Other clinical factors, such as the female sex and hypertension, may promote secondary brain damage in patients with moyamoya disease. Further evaluations of EPVS in patients with moyamoya disease are needed to better understand their pathophysiological importance.
  • Kikutaro Tokairin, Toshiya Osanai, Takeo Abumiya, Ken Kazumata, Kota Ono, Kiyohiro Houkin
    BMJ OPEN 7 8 e016502  2017年08月 [査読有り][通常論文]
     
    Introduction Acute cerebral ischaemia with main cerebral artery occlusion requires treatment with intravenous tissue plasminogen activator administration and/or endovascular thrombectomy. However, some patients fail to recover even after recanalisation because of ischaemia/reperfusion (I/R) injury. We hypothesised that regional transarterial hypothermic infusion would be effective for patients with I/R injury. The aim of this study is to validate the safety of this procedure. Methods and analysis This is a clinical exploratory study to evaluate safety of regional transarterial hypothermic infusion in combination with endovascular thrombectomy. Patients with acute ischaemic stroke and a National Institutes of Health Stroke Scale (NIHSS) score of 5-29 who require endovascular thrombectomy are eligible for the study. When no improvement in NIHSS score after the recanalisation is achieved by thrombectomy, cold saline (15 degrees C) will be administered through a microcatheter located in the ipsilateral internal carotid artery. The primary endpoints of this study are mortality and morbidity. The secondary endpoint is deleterious effects on clinical data such as symptoms, radiographic findings and physiological data. The primary and secondary endpoints will be accumulated as case series because this study will be conducted on a small sample of seven patients. Ethics and dissemination All protocols and the informed consent form comply with the Ethics Guideline for Clinical Research (Japanese Ministry of Health, Labour and Welfare). Ethics review committees at the Hokkaido University Hospital approved the study protocols. The results of the study will be disseminated at several research conferences and also contributed to peer-reviewed journals. The study will be implemented and reported in line with the SPIRIT statement.
  • Masaaki Hokari, Naoki Nakayama, Yusuke Shimoda, Kiyohiro Houkin
    WORLD NEUROSURGERY 103 431 - 441 2017年07月 [査読有り][通常論文]
     
    BACKGROUND: Some patients with aneurysm exhibit warning headaches without minor bleeding, and this could be caused by stretching of the aneurysm wall. Recently, our pathologic study observed subintimal fibrin deposition in a majority of the ruptured aneurysms. However, these findings also were observed in some unruptured aneurysms. In this report, 2 unruptured aneurysms exhibited subintimal fibrin, and interestingly, one of the patients experienced severe headache within 1 month before neuroimaging. OBJECTIVE: We performed pathologic analysis of unruptured aneurysms and collected their various clinical variables, including severe headache, to clarify the clinical characteristics of "dangerous" unruptured aneurysms. METHODS: This study included unruptured saccular aneurysm samples (n = 17) that were resected after clipping. We compared the differences in clinical variables, including warning headache, between aneurysms with and without fibrin deposition. RESULTS: Fibrin deposition was present in the subintimal layer in 4 patients and in the periouter membrane in 4 patients. Three of the 4 former patients experienced warning headaches, and one presented aneurysm growth. Of the latter 4 patients, one exhibited aneurysm growth, whereas the others presented with relatively large aneurysms. In the remaining 9 aneurysms without fibrin deposition, monocyte infiltration was observed in one, all aneurysms were small, and no patients experienced warning headaches or aneurysm growth. CONCLUSIONS: Subintimal fibrin deposition is observed frequently in patients with aneurysm with warning headaches. These pathologic findings are clinically inspiring and may suggest that these aneurysms exhibit rapid stretching by newly formed aneurysms, which can result in rupture at an early stage.
  • Hime Suzuki, Takeshi Mikami, Tomoyoshi Kuribara, Kazuhisa Yoshifuji, Katsuya Komatsu, Yukinori Akiyama, Hirofumi Ohnishi, Kiyohiro Houkin, Nobuhiro Mikuni
    JOURNAL OF NEUROSURGERY-PEDIATRICS 19 5 560 - 566 2017年05月 [査読有り][通常論文]
     
    OBJECTIVE Medullary streaks detected on fluid-attenuated inversion recovery (FLAIR) imaging have been considered to be reflected ischemic regions in pediatric moyamoya disease. The purpose of this study was to evaluate these medullary streaks both clinically and radiologically and to discuss associated pathophysiological concerns. METHODS The authors retrospectively reviewed data from 14 consecutive pediatric patients with moyamoya disease treated between April 2009 and June 2016. Clinical and radiological features and postoperative imaging changes were analyzed. In 4 patients, hyperintense medullary streaks on FLAIR imaging (HMSF) at the level of the centrum semiovale were detected. RESULTS The HMSF were coincident with hyperintense medullary streaks on a T2-weighted image, though they were not completely coincident with the vasculature on either a T2*-weighted image or contrast-enhanced CT. Analysis revealed significantly higher values in terms of MR angiography scores, number of flow voids of the basal ganglia, and the presence of the medullary artery in the group with HMSF than in those without. In contrast, the presence of white matter damage was significantly less frequent in the HMSF group. All HMSF disappeared after surgery, and the mean apparent diffusion coefficient at the same level was significantly reduced postoperatively. CONCLUSIONS Although HMSF should be associated with collateral circulation in moyamoya disease, other factors may be involved, including stagnated cerebrospinal fluid or vasogenic edema that is relevant to the impaired state of the white matter. Findings in this study provide insight into the pathophysiological basis of the perivascular space in moyamoya disease.
  • Shunsuke Terasaka, Toshiaki Taoka, Satoshi Kuroda, Nobutaka Mikuni, Toru Nishi, Hiroyuki Nakase, Yukihiko Fujii, Yasuhiko Hayashi, Jun-ichi Murata, Ken-ichiro Kikuta, Toshihiko Kuroiwa, Sachie Shimokawa, Kiyohiro Houkin
    JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE 28 5 69 - 69 2017年05月 [査読有り][通常論文]
     
    The objective of this study is to evaluate the efficacy and safety of non-suture dural closure using a novel dural substitute (GM111) consisting of polyglycolic acid felt with a fibrin-glue-coated area commensurate in size with the dural defect. This was a non-controlled, open-label, multicenter clinical trial. The efficacy evaluation endpoints were (1) GM111's intra-operative capability to close dural defects and (2) prevention of cerebrospinal fluid (CSF) leakage and subcutaneous CSF retention throughout the postoperative period (evaluated by diagnostic imaging). Patients meeting the following three preoperative and two intra-operative selection criteria were enrolled: (1) between 12 and <75 years of age; (2) the dura is surmised to be defective and in need of reconstruction; (3) informed written consent was obtained from the patient; (4) the surgical wound is class 1; and (5) the size of duraplasty is >= 0.2 cm(2) to <100 cm(2). Sixty patients were enrolled. The craniotomy site was supratentorial in 77.2%, infratentorial in 12.3% and sellar in 10.5%. The GM111 prosthesis size ranged from 0.24 to 42 cm(2). To evaluate the efficacy, intra-operative closure was confirmed by Valsalva's maneuver, water infusion, etc., in all patients. CSF leakage and subcutaneous CSF retention throughout the postoperative period were found in four patients. Adverse events for which a causal relationship with GM111 could not be ruled out occurred in 8.8% of the patients. There were no instances of postoperative infection due to GM111. GM111 showed good closure capability and safety when used for non-suture dural closure.
  • Ken Kazumata, Hiroyasu Kamiyama, Hisayasu Saito, Katsuhiko Maruichi, Masaki Ito, Haruto Uchino, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
    OPERATIVE NEUROSURGERY 13 2 213 - 222 2017年04月 [査読有り][通常論文]
     
    BACKGROUND: The posterior cerebral artery (PCA) is involved in approximately 30% of moyamoya disease (MMD) cases. However, there have been insufficient reports describing revascularization techniques in the posterior portion of the brain, particularly of direct anastomosis. OBJECTIVE: To perform a technical assessment in patients with MMD who underwent either occipital artery (OA)-PCA bypass or OA-middle cerebral artery (MCA) bypass. METHODS: Atotal of 428 revascularization procedures in 368 patients were retrospectively assessed by reviewing clinical charts and radiological data. RESULTS: Ten patients (3.5%) were treated with direct bypass after the anterior revascularization with a median interval of 30 months (range, 5 months-16 years). Seven patients were <18 years of age (average age, 17.5 +/- 15.6 years). Preoperative symptoms included transient motor deficits involving the lower extremities (n = 5), visual disturbances (n = 6), and cerebral infarctions (n = 6). A favorable outcome (modified Rankin Scale score <3) was achieved in 9 of these 10 patients. Direct anastomosis was performed in 3 hemispheres with an OA-MCA bypass and in 8 hemispheres with an OA-PCA bypass. Patency of the direct bypass was confirmed on angiogram in 7 of 7 patients who underwent conventional angiogram performed within 1 year after the surgery. None of the 10 patients demonstrated cerebral infarctions after the posterior revascularization. CONCLUSION: In MMD, symptomatic PCA regression after anterior revascularization was found predominantly in children and young adults. Direct anastomosis in the posterior portion of the brain can be successfully achieved and is effective in preventing ischemic events.
  • Masaaki Hokari, Naoki Nakayama, Ken Kazumata, Toshiya Osanai, Hideo Shichinohe, Takeo Abumiya, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 57 3 122 - 127 2017年03月 [査読有り][通常論文]
     
    There are no reports on the outcomes of clippings in patients who receive immunosuppressants, for example, due to connective tissue diseases or following organ transplantation. We thoroughly reviewed these cases focusing on the perioperative management phase. The study included 11 patients with intracranial aneurysms who were taking immunosuppressants; between 2007 and 2014. We performed 12 clipping surgeries. Their clinical records were reviewed for age and gender, aneurysms' location and size, perioperative management of the immunosuppressive drugs, and surgical complications. The study included nine females and two males, aged between 52 and 71 years (mean 60.1 +/- 8.5 years). The clinical presentation in five cases was subarachnoid hemorrhage (SAH); the aneurysm was incidentally diagnosed in six patients (7 aneurysms). The reasons for taking immunosuppressants were autoimmune disorder in nine patients and liver transplantation in two patients. Daily intake of oral immunosuppressants for the patients with liver transplantation was discontinued for 2-4 days, and no infectious complications were evidenced. The weekly course of immunosuppressive drugs for the patients with autoimmune disorder was continued in eight of nine patients. Caution must be exercised when considering the suitability of clipping for patients taking immunosuppressants, but surgery outcomes are generally favorable; when operative treatment is required, we believe it to be comparatively safe, if the perioperative management is conducted in close collaboration with the relevant departments.
  • Masaki Ito, Hideo Shichinohe, Kiyohiro Houkin, Satoshi Kuroda
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 11 2 375 - 381 2017年02月 [査読有り][通常論文]
     
    Bone marrow stromal cells (BMSC) transplantation enhances functional recovery after cerebral infarct, but the optimal delivery route is undetermined. This study was aimed to assess whether a novel cell-sheet technology non-invasively serves therapeutic benefits to ischemic stroke. First, the monolayered cell sheet was engineered by culturing rat BMSCs on a temperature-responsive dish. The cell sheet was analysed histologically and then transplanted onto the ipsilateral neocortex of rats subjected to permanent middle cerebral artery occlusion at 7 days after the insult. Their behaviours and histology were compared with those in the animals treated with direct injection of BMSCs or vehicle over 4 weeks post-transplantation. The cell sheet was 27.9+/-8.0 mu m thick and was composed of 9.8+/-2.4x10(5) cells. Cell sheet transplantation significantly improved motor function when compared with the vehicle-injected animals. Histological analysis revealed that the BMSCs were densely distributed to the neocortex adjacent to the cerebral infarct and expressed neuronal phenotype in the cell sheet-transplanted animals. These findings were almost equal to those for the animals treated with direct BMSC injection. The attachment of the BMSC sheet to the brain surface did not induce reactive astrocytes in the adjacent neocortex, although direct injection of BMSCs profoundly induced reactive astrocytes around the injection site. These findings suggest that the BMSCs in cell sheets preserve their biological capacity of migration and neural differentiation. Cell-sheet technology may enhance functional recovery after ischaemic stroke, using a less invasive method. Copyright (C) 2014 John Wiley & Sons, Ltd.
  • Shunsaku Takayanagi, Akitake Mukasa, Hirofumi Nakatomi, Hiroshi Kanno, Jun-ichi Kuratsu, Ryo Nishikawa, Kazuhiko Mishima, Atushi Natsume, Toshihiko Wakabayashi, Kiyohiro Houkin, Shunsuke Terasaka, Masahiro Yao, Nobuo Shinohara, Taro Shuin, Nobuhito Saito
    NEUROLOGIA MEDICO-CHIRURGICA 57 2 59 - 65 2017年02月 [査読有り][通常論文]
     
    von Hippel-Lindau (VHL) disease is a hereditary tumor disease in which tumors develop in multiple organs, not only as hemangioblastomas (HBs) in the central nervous system, but also as kidney tumors, pheochromocytomas, and so on. Much about the epidemiology of VHL disease remained unknown until fairly recently in Japan, leading to calls for the establishment of a VHL disease epidemiological database in Japanese. To elucidate its epidemiology in Japan, the Japanese Ministry of Health, Labour and Welfare created the VHL Disease Study Group, which was put in charge of carrying out a nationwide epidemiological survey. The survey found close to 400 Japanese VHL disease patients throughout the country. Based on those results, the VHL Disease Study Group created the VHL Disease Treatment Guideline and also a severity classification. It is thought that the prognosis of VHL disease patients can be improved by performing genetic diagnosis and careful follow-up. Accordingly, the University of Tokyo Hospital put in place an in-hospital system for implementing genomic medicine for VHL disease based on genetic diagnosis. For that system, it was especially important to establish (I) accurate genetic diagnostic techniques, (II) genetic counseling capabilities for the patients and their families, and (III) a system of cooperation among multiple departments, including urology departments, and so on. Further elucidation of the epidemiology and the development of genomic medicine are needed to improve the treatment results of VHL disease in Japan.
  • Ken Kazumata, Masaki Ito, Haruto Uchino, Hiroshi Nishihara, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 57 2 66 - 72 2017年02月 [査読有り][通常論文]
     
    The number of clinical research papers published worldwide on moyamoya disease (MMD) has increased recently. However, the majority of the literature comprises retrospective single-center studies collecting data on small numbers of patients. Several multi-center studies are ongoing in Japan; however, the current data are insufficient for comprehensively outlining the various characteristics of MMD. To enhance our knowledge on epidemiologic, vascular, and genetic aspects of MMD, a prospective multicenter registry will be established in Japan that will help to streamline clinical research as well as improve clinical treatments and long-term outcomes. Patients with MMD or secondary moyamoya syndrome referred to the participating centers will be invited to the registry. Demographic and physiological parameters, along with neuroimaging data will be collected chronologically. Clinical events, including neurological, medical, and surgical interventions will be recorded. Whole blood samples will be collected. Extra-and intracranial vascular tissue, and/or cerebrospinal fluid will also be collected from patients who undergo surgical revascularization. These biospecimens will be stored at the repositories and utilized for genome-wide association studies for identifying genetic variants, as well as tissue-specific proteomic, and/or molecular analyses. Ethics approval will be obtained at all facilities collecting biospecimens. The registry will provide descriptive statistics on functional outcomes, surgical techniques used, medications, and neurological events stratified according to patients' clinical characteristics. We expect this study to provide novel insights in the management of MMD patients and design better therapies.
  • Uchino H, Kim JH, Fujima N, Kazumata K, Ito M, Nakayama N, Kuroda S, Houkin K
    Neurosurgery 80 2 201 - 209 2017年02月 [査読有り][通常論文]
     
    Background: Whether additional indirect bypasses effectively contribute to revascularization in combined procedures remains unclear in patients with moyamoya disease. Objective: To evaluate the longitudinal changes associated with combined procedures while following up pediatric and adult patients long term and to assess whether any other clinical factors or hemodynamic parameters affected these changes to determine an optimal surgical strategy. Methods: We studied 58 hemispheres in 43 adults and 39 hemispheres in 26 children who underwent combined revascularization for moyamoya disease. To evaluate bypass development, we assessed the sizes of the superficial temporal artery and middle meningeal artery using magnetic resonance angiography. Multivariate analysis determined the effects of multiple variables on bypass development. Results: Indirect bypass (middle meningeal artery) development occurred in 95% and 78% of the pediatric and adult hemispheres, respectively. Of these, dual development of direct and indirect bypasses occurred in 54% of the pediatric hemispheres and in 47% of the adult hemispheres. Reciprocal superficial temporal artery regression occurred in 28% of the hemispheres during the transition from the postoperative acute phase to the chronic phase during indirect bypass development. Good indirect bypass development was associated with adult hemispheres at Suzuki stage 4 or greater (odds ratio, 7.4; 95% confidence interval, 1.4-39.4; P = .02). Disease onset type and preoperative hemodynamic parameters were not considered predictors for the development of surgical revascularization. Conclusion: Simultaneous direct and indirect bypass development was most frequently observed, regardless of patient age and hemodynamic status. Applying indirect bypass as an adjunct to direct bypass could maximize revascularization in adults and children.
  • Haruto Uchino, Jae-Hoon Kim, Noriyuki Fujima, Ken Kazumata, Masaki Ito, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
    NEUROSURGERY 80 2 201 - 208 2017年02月 [査読有り][通常論文]
     
    BACKGROUND: Whether additional indirect bypasses effectively contribute to revascular-ization in combined procedures remains unclear in patients with moyamoya disease. OBJECTIVE: To evaluate the longitudinal changes associated with combined procedures while following up pediatric and adult patients long term and to assess whether any other clinical factors or hemodynamic parameters affected these changes to determine an optimal surgical strategy. METHODS: We studied 58 hemispheres in 43 adults and 39 hemispheres in 26 children who underwent combined revascularization for moyamoya disease. To evaluate bypass development, we assessed the sizes of the superficial temporal artery and middle meningeal artery using magnetic resonance angiography. Multivariate analysis determined the effects of multiple variables on bypass development. RESULTS: Indirect bypass ( middle meningeal artery) development occurred in 95% and 78% of the pediatric and adult hemispheres, respectively. Of these, dual development of direct and indirect bypasses occurred in 54% of the pediatric hemispheres and in 47% of the adult hemispheres. Reciprocal superficial temporal artery regression occurred in 28% of the hemispheres during the transition from the postoperative acute phase to the chronic phase during indirect bypass development. Good indirect bypass development was associated with adult hemispheres at Suzuki stage 4 or greater ( odds ratio, 7.4; 95% confidence interval, 1.4-39.4; P = .02). Disease onset type and preoperative hemodynamic parameters were not considered predictors for the development of surgical revascularization. CONCLUSION: Simultaneous direct and indirect bypass development was most frequently observed, regardless of patient age and hemodynamic status. Applying indirect bypass as an adjunct to direct bypass could maximize revascularization in adults and children.
  • 東海林菊太郎, 東海林菊太郎, 長内俊也, 川堀真人, 川堀真人, 新谷好正, 寳金清博
    脳血管内治療(Web) 2 Supplement 2017年
  • Hisayasu Saito, Michiyuki Miyamoto, Hideo Shichinohe, Kiyohiro Houkin, Satoshi Kuroda
    Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials 111 - 119 2017年01月01日 [査読有り][通常論文]
     
    Cell transplantation therapy has been expected to promote functional recovery in various kinds of central nervous system (CNS) disorders, including cerebral stroke. However, there are several concerns to be resolved before clinical application of cell therapy for CNS disorders. The issues include the development of imaging techniques to monitor the response of the host CNS. It would be essential to establish functional bio-imaging technique serially and noninvasively validating the effects of cell therapy on the host CNS in order to achieve clinical application of cell therapy for cerebral stroke. Nuclear imaging technique is one of the most useful methods to assess the functional change in various kinds of CNS disorders, including cerebral stroke. Very recently, using a small-animal SPECT/CT apparatus, we could serially visualize the effects of BMSC transplantation on the distribution of 123I-IMZ in the infarct brain of the living rodents longitudinally and noninvasively. Furthermore, we serially assessed local glucose metabolism in the rats subjected to permanent MCA occlusion and found that BMSC transplantation significantly enhances the recovery in the peri-infarct area, using smallanimal 18F-FDG PET/CT system. The BMSCs may enhance the recovery of local glucose metabolism by improving neuronal integrity in the peri-infarct area, when directly transplanted into the infarct brain. Although there are few studies that indicate the utility of imaging techniques to monitor the response of the host CNS after cell therapy and further investigation is needed, 123I-IMZ SPECT and 18FFDG PET may be promising modalities to assess the therapeutic benefits of cell therapy for ischemic stroke without subjective bias in clinical situation.
  • Masaki Ito, Kiyohiro Houkin
    Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials 49 - 72 2017年01月01日 [査読有り][通常論文]
     
    Cell production under Good Manufacturing Practice (GMP) protocol is mandatory for the proper application of therapeutic cells in clinical settings. If cells are produced under GMP conditions, chemically defined conditions and a controlled environment would be ensured. However, such practices do not specify the use of animal-derived or xenogeneic recombinant supplements, which might raise some concern for clinical-grade cell preparations. At the very least, information of these materials should be provided to the patients treated with cell therapy to ensure proper understanding and informed assent. Therefore, in this chapter, the conventional cell culture methods employed for cell preparation (isolation, expansion, and/or derivation) are discussed, with a particular focus on each of the cell types employed in clinical trials of cell therapy against cerebral stroke.
  • Taku Sugiyama, Satoshi Kuroda, Kiyohiro Houkin
    Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials 101 - 110 2017年01月01日 [査読有り][通常論文]
     
    Cell therapy is expected to promote functional recovery in various kinds of central nervous system disorders. Many studies show beneficial effects of cell therapy, and several clinical studies have already been initiated worldwide. Although these results are encouraging, several problems remain, including elucidating the therapeutic mechanisms, treatment timing, optimal cell dose, type of cells, and cell delivery route. For further optimization of this therapy, it is essential to develop in vivo cell tracking techniques. Longitudinal and serial analyses of the fate of transplanted cells are quite important for solving these problems. There are several cell labeling techniques and imaging modalities, including magnetic resonance imaging, nuclear imaging, and optical imaging. However, any single imaging modality has its own distinct advantages and drawbacks. Proper understanding of each technique’s characteristics is crucial for successful in vivo imaging. In this chapter, we present a literature survey of cell tracking techniques used in clinical settings and laboratories and introduce recent advances in this field.
  • Satoshi Kuroda, Hideo Shichinohe, Kiyohiro Houkin
    Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials 15 - 25 2017年01月01日 [査読有り][通常論文]
     
    In this article, the authors review recent advancements and perspective on cell therapy for ischemic stroke with bone marrow-derived cells, including bone marrow stromal cells (BMSCs) and multilineage-differentiating stress-enduring (Muse) cells. They can be easily isolated from the patients themselves and transplanted into them without any ethical and immunological problem. Animal experiments have shown that direct transplantation of these adult stem cells significantly enhances the recovery of motor function in various types of neurological disorders, including ischemic stroke. They aggressively migrate toward the damaged tissue and proliferate in the host brain. The BMSCs may contain heterogeneous subpopulations and contribute to functional recovery through multiple mechanisms, including neuroprotection, inflammatory modulation, cell fusion, and neural differentiation. On the other hands, Muse cells may promote functional recovery after ischemic stroke by reorganizing the infarct brain.
  • Toru Sasamori, Kazutoshi Hida, Toshiya Osanai, Shunsuke Yano, Toshitaka Seki, Kiyohiro Houkin
    NEUROSURGICAL REVIEW 40 1 83 - 86 2017年01月 [査読有り][通常論文]
     
    Neurological improvement in patients with spinal dural arteriovenous fistulae (SDAVF) is often partial even after adequate treatment. While treatment outcomes have been evaluated primarily on the basis of the postoperative changes in neurological deficits, outcome measures should also reflect the patient-reported outcome (PRO). We conducted a health-related quality of life (HRQOL) survey in 52 SDAVF patients; 33 (63.5%) completed the short-form 36 Health Survey (SF-36) questionnaire. They were 25 males and 8 females ranging in age from 47 to 85 years (mean age 70.0 years). The mean follow-up period was 95.6 months. We analyzed the completed questionnaires and examined the clinical factors associated with their HRQOL. After treatment, gait- and micturition disturbances persisted in 31 (93.9%) and 31 (93.9%) of our patients; 26 (78.8%) reported chronic leg pain. The SF-36 scores of treated SDAVF patients were significantly lower than the national average of 50 for all 8 sub-items in the questionnaire. The scores for physical functioning (PF) and role-physical (RP) were particularly low. With the exception of bodily pain (BP), there was a significant negative correlation between the Aminoff-Logue scale (ALS) scores for gait- and micturition and the sub-item scores. The score for BP showed a significant positive correlation with the scores for the 7 other SF-36 sub-items. The HRQOL of treated SDAVF patients was lower than the national average with respect to both physical and mental aspects. Persistent post-treatment pain and gait- and micturition disturbances were responsible for their lower HRQOL.
  • Takamiya S, Sasamori T, Hamauchi S, Seki T, Kano T, Yabe I, Sasaki H, Houkin K
    No shinkei geka. Neurological surgery 45 1 53 - 58 2017年01月 [査読有り][通常論文]
     
    We report two patients in whom the intrathecal baclofen(ITB)catheter was located in the subdural space, although we had confirmed good outflow of spinal fluid from the spinal catheter. Patient 1 was a woman in her 30s with spastic quadriplegia due to subarachnoid hemorrhage. An ITB pump was implanted, and a good outflow of spinal fluid from the spinal catheter was observed during the surgery. Postoperatively, her spasticity did not improve. Catheter myelography revealed that the spinal catheter was located in the subdural space. Using intraoperative catheter myelography, we corrected the position of the catheter. Patient 2 was a man in his 20s diagnosed with adrenoleukodystrophy. An ITB therapy was performed to improve his spastic gait. Intraoperative catheter myelography showed that the spinal catheter was located in the subdural space, although there was good outflow of spinal fluid from the catheter. Our experience suggests that the outflow of spinal fluid alone should not be used to determine the location of the spinal catheter. Intraoperative catheter myelography is useful for the correct placement of the spinal catheter in the subarachnoid space.
  • Takeshi Mikami, Tomoyoshi Kuribara, Katsuya Komatsu, Yusuke Kimura, Masahiko Wanibuchi, Kiyohiro Houkin, Nobuhiro Mikuni
    NEUROLOGICAL RESEARCH 39 8 702 - 708 2017年 [査読有り][通常論文]
     
    Objectives: Meandering flow void around the splenium, which can be recognized on conventional T2-weighted images, implicates collateral flow in the splenial artery in patients with moyamoya disease. In this report, curves of flow voids around the splenium (SFVs) were evaluated in patients with moyamoya disease, and their diagnostic value and pathophysiology were verified. Methods: A total of 65 consecutive patients with moyamoya disease were included in this analysis. The number of SFV curves was counted on each side. The numbers of SFV curves in patients with moyamoya disease was compared with those in the control group. The clinical features of patients with large numbers of flow voids were analyzed. Results: The mean number of SFVs was significantly higher in the moyamoya disease group than in the control group or the atherosclerotic disease group. The cut-off SFV count for a diagnosis of moyamoya disease should be set to 2.25 in consideration of receiver operating characteristic curve analysis. A multivariate logistic analysis of SFV data in moyamoya disease revealed significant differences between the high SFV count group (2.25) and the low SFV count group (<2.25) in age, flow voids in the basal ganglia, and magnetic resonance angiography score of the internal carotid artery and anterior cerebral artery. Conclusion: Based on this study, number of SFV curves on conventional T2-weighted imaging could be a useful measurement for confirming a diagnosis of moyamoya disease. Increased number of SFV curves was associated with age and progress of disease.
  • Furuse M, Nonoguchi N, Kuroiwa T, Miyamoto S, Arakawa Y, Shinoda J, Miwa K, Iuchi T, Tsuboi K, Houkin K, Terasaka S, Tabei Y, Nakamura H, Nagane M, Sugiyama K, Terasaki M, Abe T, Narita Y, Saito N, Mukasa A, Ogasawara K, Beppu T, Kumabe T, Nariai T, Tsuyuguchi N, Nakatani E, Kurisu S, Nakagawa Y, Miyatake SI
    Neuro-oncology practice 3 4 272 - 280 2016年12月 [査読有り][通常論文]
     
    BACKGROUND: Brain radiation necrosis (BRN) can be a complication of radiotherapy for primary and secondary brain tumors, as well as head and neck tumors. Since vascular endothelial growth factor (VEGF) is also a vascular permeability factor in the brain, bevacizumab, a humanized antibody that inhibits VEGF, would be expected to reduce perilesional edema that often accompanies BRN. METHODS: Patients with surgically untreatable, symptomatic BRN refractory to conventional medical treatments (eg, corticosteroid, anticoagulants, or hyperbaric oxygen therapy) were enrolled. We judged that a major cause of perilesional edema with a lesion-to-normal brain ratio ≤1.8 on 11C-methionine or ≤2.5 on 18F-boronophenylalanine PET was BRN, not tumor recurrence, and 6 cycles of biweekly bevacizumab (5 mg/kg) were administered. The primary endpoint was a ≥30% reduction from the patients' registration for perilesional edema continuing for ≥1 month. RESULTS: Of the 41 patients enrolled, 38 were fully eligible for the response assessment. The primary endpoint was achieved in 30 of the 38 (78.9%) patients at 3.0 months (median) after enrollment. Sixteen patients (42.1%) experienced improvement of their Karnofsy Performance Score. Corticosteroid use could be reduced in 29 patients (76.3%). Adverse events at grade ≥3 occurred in 10 patients (24.4%). CONCLUSIONS: Bevacizumab treatment offers certain clinical benefits for patients with surgically untreatable, symptomatic BRN. The determination of BRN using amino-acid PET, not biopsy, is adequate and less invasive for determining eligibility to receive bevacizumab.
  • Shigeru Yamaguchi, Kenji Hirata, Takuya Toyonaga, Kentaro Kobayashi, Yukitomo Ishi, Hiroaki Motegi, Hiroyuki Kobayashi, Tohru Shiga, Nagara Tamaki, Shunsuke Terasaka, Kiyohiro Houkin
    PLOS ONE 11 12 e0167917  2016年12月 [査読有り][通常論文]
     
    BackgroundBevacizumab (BEV), a humanized monoclonal antibody, become a currently important chemotherapeutic option for the patients with recurrent glioma. The aim of this retrospective study is to investigate whether 18 F-Fluoromisonidazole (FMISO) PET have the potential to detect BEV-resistant gliomas in the early-stage.MethodsWe reviewed the FMISO PET and MRI appearances before and 3 to 4 courses after BEV treatment on 18 recurrent glioma patients. FMISO accumulation was assessed by visual inspection and semi-quantitative values which were tumor-to-normal (T/N) ratio and hypoxic volume. MRI responses were evaluated based on RANO (Response Assessment in Neuro-Oncology) criteria. The prognostic analysis was performed in relation to the response assessment by FMISO PET and MRI using overall survival (OS) after BEV application.ResultsAfter BEV application, MRI revealed partial response in 14 of 18 patients (78%), of which 9 patients also demonstrated decreased FMISO accumulation. These 9 patients (50%) were classified as "MRI-FMISO double responder". As for the other 5 patients (28%), FMISO accumulation volumes increased or remained stable after BEV treatment although partial responses were achieved on MRI. Therefore, these cases were classified as "MRI-only responder". The remaining 4 patients (22%) did not show treatment response on FMISO PET or MRI ("non-responder"). MRI-FMISO double responders showed significantly longer OS than that in other groups (median 12.4 vs 5.7 months; P < 0.001), whereas there were no overall survival difference between MRI-only responders and non-responders (median OS, 5.7 and 4.8 months; P = 0.58). Among the pre-treatment clinical factors, high FMISO T/N ratio was a significant prognostic factor of overall survival in these patients under the assessment of Cox proportional hazard model.ConclusionsRecurrent gliomas with decreasing FMISO accumulation after short-term BEV application could derive a survival benefit from BEV treatment. Change in FMISO PET appearance can identify BEV-resistant gliomas in early-stage regardless of MRI findings in a comprehensible way.
  • Koji Furukawa, Takeo Abumiya, Keiji Sakai, Miki Hirano, Toshiya Osanai, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Kazutoshi Hida, Kiyohiro Houkin
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 25 11 2762 - 2769 2016年11月 [査読有り][通常論文]
     
    Background and Purpose: High blood viscosity causes blood stagnation and subsequent pathological thrombotic events, resulting in the development of ischemic stroke. We hypothesize that the contribution of blood viscosity may differ among ischemic stroke subtypes based on specific pathological conditions. We tried to verify this hypothesis by measuring blood viscosity in acute ischemic stroke patients using a newly developed electromagnetic spinning sphere (EMS) viscometer. Methods: Measurements in acute ischemic stroke patients were performed 4 times during admission and data were compared with those obtained from 100 healthy outpatient volunteers. Results: We enrolled 92 patients (cardioembolism: 25, large artery atherosclerosis: 42, and small artery occlusion [SAO]: 25) in this study. Comparisons of blood viscosity between the ischemic stroke subgroups and control group revealed that blood viscosity at the date of admission was significantly higher in the SAO group (5.37 +/- 1.11 mPa.s) than in the control group (4.66 +/- .72 mPa.s) (P <.01). Among all subtype groups showing a reduction in blood viscosity after 2 weeks, the SAO group showed the highest and most significant reduction, indicating that SAO patients had the most concentrated blood at the onset. Conclusions: Blood viscosity was significantly increased in the SAO group at the date of admission, which indicated the contribution of dehydration to the onset of ischemic stroke. The importance of dehydration needs to be emphasized more in the pathogenesis of SAO. The clinical application of the EMS viscometer is promising for understanding and differentiating the pathogenesis of ischemic stroke.
  • Kota Kurisu, Takeo Abumiya, Masaki Ito, Masayuki Gekka, Toshiya Osanai, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
    BRAIN RESEARCH 1651 95 - 103 2016年11月 [査読有り][通常論文]
     
    The robust neuroprotective effects of transarterial regional hypothermia have been demonstrated in the typical transient middle cerebral artery occlusion (tMCAO) model, but have not yet been tested in other ischemic stroke models, even though clinical ischemic conditions are diverse. In order to clarify these effects in a different ischemic stroke model, we employed a rat model of permanent MCAO (pMCAO) with transient collateral hypoperfusion (tCHP), which was achieved by direct MCA ligation through craniotomy and 1-h bilateral common carotid artery occlusion at the beginning of pMCAO. The infusion of 20 ml/kg of 4 degrees C cold saline (CS) or 37 degrees C warm saline (WS) into the ipsilateral internal carotid artery (ICA) was performed for 15 min in intra- or post-tCHP. Neurological scores, infarct/edema volumes, and neuronal apoptosis and reactive gliosis were compared between the CS and WS groups and a non-infusion control group after 48 h of reperfusion. Although brain temperatures were only reduced by 2-3 degrees C for 15 min, the CS group had significantly better neurological scores, smaller infarct/edema volumes, and less penumbral neuronal apoptosis and reactive gliosis than the control and WS groups. The post-tCHP CS group exhibited prominent neuroprotective effects, even though infarct volumes and neuronal apoptosis were reduced less than those in the intra-tCHP CS group. In conclusion, we demonstrated the neuroprotective effects of transarterial regional hypothermia in an ischemic model of pMCAO with tCHP. Even though MCAO is persistent, cold infusion via the ICA is neuroprotective for the penumbra, suggesting the wider therapeutic application of this therapy. (C) 2016 Elsevier B.V. All rights reserved.
  • Shunsuke Terasaka, Katsuyuki Asaoka, Shigeru Yamaguchi, Hiroyuki Kobayashi, Hiroaki Motegi, Kiyohiro Houkin
    NEUROSURGICAL REVIEW 39 4 607 - 612 2016年10月 [査読有り][通常論文]
     
    Although microvascular decompression (MVD) is a reliable treatment for hemifacial spasm (HFS), the postoperative course is varied. We retrospectively analyzed the resolution pattern of the spasm and specified predictors for delayed cure after MVD. This study included 114 consecutive patients with typical HFS. All of them were followed up for at least 1 year after operation. Patients were divided into three groups depending on the postoperative course: immediate cure, delayed cure, and failure. To identify the predictive factors for delayed cure after MVD, logistic regression analyses were applied using candidate clinical factors, such as duration of symptom, the tendency of the spasm, preoperative medical treatment, and offending vessels. Among the 114 patients, 107 patients were cured. For those cured, 65 patients were classified as immediate cure and 42 patients were classified as delayed cure. Cumulative spasm-free rates after 1 week, 1 month, and 3 months after MVD were 70, 88, and 97 %, respectively. No predictive factors between the cured and failure groups were observed. According to multivariate analysis, preoperative anticonvulsant therapy was found to be the sole significant predictive factor for delayed cure after MVD (p = 0.025). A significant correlation between delayed cure and preoperative anticonvulsant therapy was found in our study, which suggests that hyperexcitation of the facial nucleus plays an important role in pathogenesis of delayed cure. Therefore, if a patient demonstrating a positive response to preoperative anticonvulsant therapy showed a persistent spasm after MVD, reoperation should be delayed for at least 3 months after the initial operation.
  • Yukitomo Ishi, Shunsuke Terasaka, Shigeru Yamaguchi, Michiharu Yoshida, Shogo Endo, Hiroyuki Kobayashi, Kiyohiro Houkin
    WORLD NEUROSURGERY 94 80 - 88 2016年10月 [査読有り][通常論文]
     
    BACKGROUND: To evaluate the accuracy of tumor size by maximum diameter, ABC/2 formula, and planimetry method using thick-slice and thin-slice magnetic resonance imaging (MRI). METHODS: Maximum diameter and tumor volume calculated using ABC/2 formula (V1) and planimetry method with thick-slice MRI (V2) and thin-slice MRI (V3) were examined in 83 meningiomas. Form factor (FF) analysis was performed to assess irregularity of the tumor. V3 values were considered as real tumor volumes. The accuracy of V1 and V2 was evaluated using ratio and difference from V3. Meningiomas were categorized by tumor locations: skull base (anterior, middle, and posterior) and noneskull base (calvaria and other sites). RESULTS: Correlation between maximum diameter and V3 was statistically significant (r =0.91), but the error was significant in tumors with longer maximum diameters. Correlation between V1 and V3 was significant (r =0.97). However, V1 tended to be larger in middle skull base meningiomas or in tumors with low FF values (R-2 =0.21). V2 represented relatively accurate volumes in both groups except in the case of small meningiomas. When tumors were demonstrated within 3 fractions on thick-slice MRI, the ratio of V2 to V3 showed significant variability. CONCLUSIONS: Using the ABC/2 formula, the volume of meningiomas in the middle skull base or meningiomas with low FF value might be calculated larger than the real tumor volume. The planimetry method with thick-slice MRI demonstrated relatively accurate volumes if the tumor was fractionated in > 4 slices.
  • Noriyuki Fujima, Toshiya Osanai, Yukie Shimizu, Atsushi Yoshida, Taisuke Harada, Naoki Nakayama, Kohsuke Kudo, Kiyohiro Houkin, Hiroki Shirato
    JOURNAL OF MAGNETIC RESONANCE IMAGING 44 4 834 - 845 2016年10月 [査読有り][通常論文]
     
    PurposeTo evaluate the utility of a vessel-selective four-dimensional (4D) magnetic resonance angiography (MRA) technique for the evaluation of intracranial arteriovenous malformations (AVMs). Materials and MethodsTwelve AVM patients were evaluated retrospectively. Time-of-flight (TOF) MRA, nonvessel-selective 4D-MRA (NS-4D-MRA), and vessel-selective 4D-MRA (VS-4D-MRA) were performed using a 3T MR unit in all patients, and used to identify feeding arteries and draining veins and measure nidus size. The diagnostic accuracy of the three techniques was compared using digital subtraction angiography (DSA). If a multifeeder was observed, the percentage of blood flow of each feeding artery to the entire nidus was evaluated and compared to the DSA findings using the error value, defined as the degree of overestimation of the blood flow. All imaging findings were assessed by two neuroradiologists. ResultsIn both raters, the detectability of feeding arteries by VS-4D-MRA (12 and 11 patients) was significantly higher than those of TOF-MRA (7 and 6 patients) and NS-4D-MRA (8 and 7 patients) (P < 0.016). The detectability of drainer veins by TOF-MRA (10 and 10 patients) was significantly higher than that of VS-4D-MRA (7 and 6 patients). In the percentage of the blood flow of each feed artery to the entire nidus, the DSA findings (error value; 27.15.7) indicated overestimations of the blood flow compared to the VS-4D-MRA (error value; 7.1 +/- 3.9) (P < 0.001). ConclusionVS-4D-MRA was shown to be a useful technique for the evaluation of intracranial AVMs, especially for detecting feed arteries and estimating details of the nidus structure. J. MAGN. RESON. IMAGING 2016;44:834-845.
  • Shuji Hamauchi, Hideo Shichinohe, Haruto Uchino, Shigeru Yamaguchi, Naoki Nakayama, Ken Kazumata, Toshiya Osanai, Takeo Abumiya, Kiyohiro Houkin, Takumi Era
    PLOS ONE 11 9 e0163561  2016年09月 [査読有り][通常論文]
     
    Background and purpose Moyamoya disease (MMD) is a slow, progressive steno-occlusive disease, arising in the terminal portions of the cerebral internal carotid artery. However, the functions and characteristics of the endothelial cells (ECs) in MMD are unknown. We analyzed these features using induced pluripotent stem cell (iPSC)-derived ECs. Methods iPSC lines were established from the peripheral blood of three patients with MMD carrying the variant RNF213 R4810K, and three healthy persons used as controls. After the endothelial differentiation of iPSCs, CD31(+)CD144(+) cells were purified as ECs using a cell sorter. We analyzed their proliferation, angiogenesis, and responses to some angiogenic factors, namely VEGF, bFGF, TGF-beta, and BMP4. The ECs were also analyzed using DNA microarray and proteomics to perform comprehensive gene and protein expression analysis. Results Angiogenesis was significantly impaired in MMD regardless of the presence of any angiogenic factor. On the contrary, endothelial proliferation was not significant between controland MMD-derived cells. Regarding DNA microarray, pathway analysis illustrated that extracellular matrix (ECM) receptor-related genes, including integrin beta 3, were significantly down-regulated in MMD. Proteomic analysis revealed that cytoskeleton-related proteins were downregulated and splicing regulation-related proteins were upregulated in MMD. Conclusions Downregulation of ECM receptor-related genes may be associated with impaired angiogenic activity in ECs derived from iPSCs from patients with MMD. Upregulation of splicing regulation-related proteins implied differences in splicing patterns between control and MMD ECs.
  • Koji Furukawa, Takeo Abumiya, Keiji Sakai, Miki Hirano, Toshiya Osanai, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Toshimitsu Aida, Kiyohiro Houkin
    Journal of medical engineering & technology 40 6 285 - 92 2016年08月 [査読有り][通常論文]
     
    We herein applied an electromagnetic spinning sphere (EMS) viscometer to the measurement of human blood viscosity for the first time. We collected blood samples from 100 healthy outpatient volunteers in order to analyse viscosity dependence on blood cell parameters and on the shear rate with a simple approximation formula [ηi (γ)\, = Ai γ(- pi) + η0]. Viscosity dependence on blood cell parameters was relatively high at a high shear rate, but became lower as the shear rate decreased. The approximation formula with appropriate parameters of Ai and pi nearly faithfully reproduced actual blood rheological behaviour with a standard deviation of 1.5%. The distributions of Ai and pi values were broad, suggesting that the pattern of viscosity dependence on the shear rate varied with individual differences. The results obtained using the EMS viscometer suggest that blood viscosity values are individual-specific and actual individual measurements are important for understanding rheological conditions.
  • Masaaki Hokari, Ken Kazumara, Naoki Nakayama, Satoshi Ushikoshi, Taku Sugiyama, Katsunori Asaoka, Kazuki Uchida, Daisuke Shimbo, Koji Itamoto, Yuka Yokoyama, Masanori Isobe, Tetsuaki Imai, Toshiya Osanai, Kiyohiro Houkin
    WORLD NEUROSURGERY 92 434 - 444 2016年08月 [査読有り][通常論文]
     
    OBJECTIVE: There are no established treatment strategies for aneurysms that recur after clipping. In this study, we present cases of patients who experienced recurrent aneurysms after clipping and subsequently underwent surgical intervention. METHODS: Between 2004 and 2015, we surgically treated 23 aneurysms that recurred at a previously clipped site. Patient characteristics and clinical history were retrospectively reviewed. RESULTS: Patients included 19 women and 4 men 45-81 years old. Aneurysms recurred 3-31 years (mean, 15.4 years) after the initial operation. For 18 cases, the first clinical presentation was a subarachnoid hemorrhage; aneurysms were incidentally diagnosed in 5 patients. Aneurysm locations were as follows: 9 on the internal carotid artery; 4 on the middle cerebral artery; 7 on the anterior communicating artery; 2 on the distal anterior cerebral artery; and 1 on the basilar artery. The reasons for retreatment included subarachnoid hemorrhage (n = 9) and aneurysm regrowth detected on follow-up examinations (n = 14). Endovascular treatment was performed in 10 cases, and direct surgery was performed in 13 cases (clipping in 8, clipping or trapping with bypass in 5). Various complex vascular reconstructions, including high-flow bypass and intracranial-intracranial in situ bypass, were performed for recurrent aneurysms. CONCLUSIONS: In our experience, coil embolization is a safe and effective procedure for treating recurrent aneurysms. When cases are unsuitable for coil embolization, surgical treatment often requires neurosurgeons not only to overcome the general technical difficulty of reoperative clipping but also to perform challenging vascular reconstruction.
  • Kota Kurisu, Takeo Abumiya, Hideki Nakamura, Daisuke Shimbo, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Hiroshi Shimizu, Kiyohiro Houkin
    NEUROSURGERY 79 1 125 - 134 2016年07月 [査読有り][通常論文]
     
    BACKGROUND: Although transarterial regional hypothermia is an attractive alternative to general hypothermia, its efficacy and underlying mechanisms remain unclear. OBJECTIVE: To confirm transarterial regional hypothermia therapeutic effects on ischemia/reperfusion (I/R) injury and to elucidate the mechanisms responsible. METHODS: The therapeutic effects of transarterial regional hypothermia were initially investigated in 2-hour middle cerebral artery occlusion rats regionally infused with 10 degrees C saline (cold saline group) or 37 degrees C saline (warm saline group) and untreated rats (control group) just before the onset of 24 hours of reperfusion. The time course of infarct and edema progression, inflammatory reactions, microvascular morphological changes, and aquaporin-4 (AQP4) expression was analyzed after 0, 2, 6, and 24 hours of reperfusion. RESULTS: Cold saline infusion only lowered brain temperatures for 30 minutes but mediated strong neuroprotective effects with infarct volume reductions of less than one- third. The time-course analysis revealed the following sequence of ischemia/reperfusion injury-related events in the control group: upregulated expression of AQP4 (2 hours); microvascular narrowing resulting from swollen astrocytic end-feet (2-6 hours); infarct and edema progression, blood-brain barrier disruption, and upregulated expression of intracellular adhesion molecule-1 (6-24 hours); and the activation of other inflammatory reactions (24 hours). These sequential events were inhibited in the cold saline group. CONCLUSION: Transarterial regional hypothermia initially inhibited the acute AQP4 surge and then attenuated microvascular narrowing, blood-brain barrier disruption, and activation of other inflammatory reactions, leading to strong neuroprotective effects. More direct and intensive cooling of the endothelium and its surroundings may contribute to these effects.
  • Haruto Uchino, Naoki Nakayama, Ken Kazumata, Satoshi Kuroda, Kiyohiro Houkin
    STROKE 47 7 1930 - 1932 2016年07月 [査読有り][通常論文]
     
    Background and Purpose-Postoperative hyperperfusion-related transient neurological deficits (TNDs) are frequently observed in adult patients with moyamoya disease who undergo direct bypass procedures. The present study evaluated the effect of the free radical scavenger edaravone on postoperative hyperperfusion in adult moyamoya disease. Methods-This study included 92 hemispheres in 72 adult patients who underwent direct bypass for moyamoya disease. Serial measurements of cerebral blood flow were conducted immediately after surgery and on postoperative days 2 and 7. In 40 hemispheres for 36 patients, edaravone (60 mg/d) was administered from the day of surgery to postsurgical day 7. The incidence of postoperative hyperperfusion and associated TNDs were compared with a control group that included 52 hemispheres in 36 patients. Results-Radiological hyperperfusion was observed in 28 of 40 (70.0%) and 39 of 52 (75.0%) hemispheres in the edaravone and control groups, respectively (P=0.30). Hyperperfusion-related TND incidences were significantly lower in the edaravone group compared with the control group (12.5% versus 32.7%; P=0.024). Multivariate analysis demonstrated that edaravone administration (P=0.009) and left-sided surgery (P=0.037) were significantly correlated with hyperperfusion-related TNDs (odds ratios, 0.3 and 4.2, respectively). Conclusions-Perioperative administration of edaravone reduced the incidence of hyperperfusion-related TNDs after direct bypass procedures in adult patients with moyamoya disease.
  • Yusuke Shimoda, Toshiya Osanai, Syunsuke Terasaka, Hiroyuki Kobayashi, Shigeru Yamaguchi, Shogo Endo, Kanako Hatanaka, Kiyohiro Houkin
    No shinkei geka. Neurological surgery 44 7 555 - 60 2016年07月 [査読有り][通常論文]
     
    Embosphere® was approved in Japan for embolizing intracranial arteries for the reduction of intraoperative bleeding in January 2014. Until August 2015, we performed embolization for four meningiomas using Embosphere®. We performed an initial evaluation of all the cases, including evaluation of the clinical courses, change in the maximum tumor diameters and volumes, alteration in the appearance on magnetic resonance imaging(MRI), amount of intraoperative bleeding, complications, and histopathological findings. After embolization, the maximum tumor diameters and volumes slightly decreased on MRI, whereas the signal change on diffusion-weighted imaging(DWI)or fluid-attenuated inversion recovery(FLAIR)varied in each case. One case demonstrated a partial signal change on DWI one day after the initial procedure, and another case demonstrated a decrease in perifocal edema on FLAIR. Among our patients, least bleeding was recorded at 6 days after the embolization. Histopathological analysis revealed coagulative necrotic lesions in two cases. The von Kossa stain was used to distinguish Embosphere® from the psammoma body. One case involved a thromboembolic complication of the retinocentral artery caused by an Embosphere® of 100-300 μm. Our early-stage experience suggests that an Embosphere® of 300-500 μm should be used for safe embolization before resection to avoid thromboembolic complications because an Embosphere® of 100-300 μm can pass through the dangerous anastomosis. Our present strategy was to resect the tumor approximately seven days after the embolization using Embosphere®. However, further studies and discussion on the size of Embosphere®, and the interval between pre-operative embolization and surgical removal are needed.
  • Yukitomo Ishi, Shigeru Yamaguchi, Akihiro Iguchi, Yuko Cho, Junjiro Ohshima, Kanako C. Hatanaka, Emi Takakuwa, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
    JOURNAL OF NEUROSURGERY-PEDIATRICS 18 1 41 - 45 2016年07月 [査読有り][通常論文]
     
    Primary intracranial rhabdomyosarcoma is quite rare, and its prognosis is poor compared with that for rhabdomyosarcoma in other organs. The authors present a case of pineal rhabdomyosarcoma successfully managed with multimodal therapy including surgery, chemotherapy, radiation, and high-dose chemotherapy (HDC) followed by autologous peripheral blood stem cell transplantation (HDC/APBSCT). An 8-year-old girl presenting with headache and nausea was referred to the authors' institution. Computed tomography and MRI revealed a pineal tumor associated with obstructive hydrocephalus. Subsequently, an emergent endoscopic tumor biopsy and third ventriculostomy were performed. The patient's symptoms immediately improved. The most likely pathological diagnosis was embryonal rhabdomyosarcoma. Chemotherapy with etoposide, cyclophosphamide, cisplatin, pirarubicin, ifosfamide, actinomycin D, and vincristine was followed by a second-look operation and whole-brain and craniospinal radiation. Because the intraoperative findings and pathological examination of the second operation suggested a definitive diagnosis of rhabdomyosarcoma and the presence of viable residual tumor cells, HDC with etoposide and melphalan was followed by APBSCT. The patient was discharged from the hospital without residual tumor or any neurological deficit. No recurrence was observed at 30 months. This is the first case of primary pineal rhabdomyosarcoma treated with HDC/APBSCT. Although the efficacy of HDC/APBSCT for rhabdomyosarcoma has not been established, the prognosis of primary intracranial rhabdomyosarcoma treated with conventional treatment is quite poor. High-dose chemotherapy followed by APBSCT may contribute to a better prognosis for primary intracranial rhabdomyosarcoma.
  • Sayaka Yuzawa, Hiroshi Nishihara, Shigeru Yamaguchi, Hiromi Mohri, Lei Wang, Taichi Kimura, Masumi Tsuda, Mishie Tanino, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin, Norihiro Sato, Shinya Tanaka
    MODERN PATHOLOGY 29 7 708 - 716 2016年07月 [査読有り][通常論文]
     
    Recent genetic analyses using next-generation sequencers have revealed numerous genetic alterations in various tumors including meningioma, which is the most common primary brain tumor. However, their use as routine laboratory examinations in clinical applications for tumor genotyping is not cost effective. To establish a clinical sequencing system for meningioma and investigate the clinical significance of genotype, we retrospectively performed targeted amplicon sequencing on 103 meningiomas and evaluated the association with clinicopathological features. We designed amplicon-sequencing panels targeting eight genes including NF2 (neurofibromin 2), TRAF7, KLF4, AKT1, and SMO. Libraries prepared with genomic DNA extracted from PAXgenefixed paraffin-embedded tissues of 103 meningioma specimens were sequenced using the Illumina MiSeq. NF2 loss in some cases was also confirmed by interphase-fluorescent in situ hybridization. We identified NF2 loss and/or at least one mutation in NF2, TRAF7, KLF4, AKT1, and SMO in 81 out of 103 cases (79%) by targeted amplicon sequencing. On the basis of genetic status, we categorized meningiomas into three genotype groups: NF2 type, TRAKLS type harboring mutation in TRAF7, AKT1, KLF4, and/or SMO, and 'not otherwise classified' type. Genotype significantly correlated with tumor volume, tumor location, and magnetic resonance imaging findings such as adjacent bone change and heterogeneous gadolinium enhancement, as well as histopathological subtypes. In addition, multivariate analysis revealed that genotype was independently associated with risk of recurrence. In conclusion, we established a rapid clinical sequencing system that enables final confirmation of meningioma genotype within 7 days turnaround time. Our method will bring multiple benefits to neuropathologists and neurosurgeons for accurate diagnosis and appropriate postoperative management.
  • 関 俊隆, 飛騨 一利, 矢野 俊介, 笹森 徹, 濱内 祝嗣, 寶金 清博
    小児の脳神経 41 1 126 - 126 (一社)日本小児神経外科学会 2016年05月
  • Shuji Hamauchi, Toshitaka Seki, Toru Sasamori, Kiyohiro Houkin
    JOURNAL OF NEUROSURGERY-PEDIATRICS 17 5 540 - 543 2016年05月 [査読有り][通常論文]
     
    Intermediate incisions are considered necessary to pass a catheter tube from the head to the abdomen in ventriculo-peritoneal (VP) shunting via a frontal bur hole. However, an intermediate incision can sometimes become dehiscent, resulting in CSF leakage or infection of the shunt system in the early period after shunt implantation, particularly in infant. patients. In this article, the authors describe a novel method of VP shunt insertion that does not require an intermediate incision. This nonintermediate-incision VP shunt procedure was performed in 3 infant patients with hydrocephalus and was not associated with any complications. This method can eliminate the intermediate incision, which is a disadvantage of VP shunt insertion via a frontal bur hole.
  • Ayse Kacar Bayram, Ebru Yilmaz, Huseyin Per, Masaki Ito, Haruto Uchino, Selim Doganay, Kiyohiro Houkin, Ekrem Unal
    CHILDS NERVOUS SYSTEM 32 3 569 - 573 2016年03月 [査読有り][通常論文]
     
    Moyamoya disease is an uncommon, progressive, and occlusive cerebrovascular disorder, predominantly affecting the terminal segment of the internal carotid arteries and its main branches. This occlusion results at the formation of a compensatory collateral arterial network (moyamoya vessels) developing at the base of the brain. The c.14576G > A variant in ring finger protein 213 (RNF213) was recently reported as a susceptibility gene for moyamoya disease. We describe two Turkish pediatric siblings with moyamoya disease born to consanguineous, unaffected Turkish parents. The first patient (proband) is a 2-year-old boy who presented with afebrile focal seizures, moderate psychomotor retardation, paresis in the left upper and lower extremity, multiple infarctions of the brain, stenosis of the bilateral internal carotid artery and the middle cerebral artery, and stenosis of the right posterior cerebral artery. The second patient is a 10-year-old girl who is an elder sister of proband. She showed normal psychomotor development, millimetric signal enhancement without diffusion limitation of the brain, and stenosis of the bilateral internal carotid artery. We herein report pediatric sibling patients of moyamoya disease who have homozygous wild-type c.14576G > A variant in RNF213, showing different clinical course and disease severity. This is the first report of pediatric siblings with moyamoya disease from Turkey validating the genetic background of most frequent variant in East Asian patients with moyamoya disease.
  • Yusuke Shimoda, Toshiya Osanai, Naoki Nakayama, Satoshi Ushikoshi, Masaaki Hokari, Hideo Shichinohe, Takeo Abumiya, Ken Kazumata, Kiyohiro Houkin
    Journal of neurosurgery. Pediatrics 17 3 330 - 5 2016年03月 [査読有り][通常論文]
     
    Hereditary hemorrhagic telangiectasia (HHT) is an autosomal dominant systemic disorder characterized by the enlargement of capillaries, recurrent nosebleeds, and multiple arteriovenous malformations (AVMs). Although cerebral AVMs are traditionally considered to be congenital lesions, some reports have described de novo AVMs, which suggests that the authors believed them to be dynamic conditions. In this article, the authors describe the case of a 5-year-old boy with HHT in whom a de novo cerebral AVM was detected after a negative MRI result at 5 months. To the authors' knowledge, this is the first report of a de novo AVM in a patient with HHT. In patients with a family history of HHT, de novo AVMs are possible, even when no lesions are detected at the first screening. Therefore, regular screenings need to be performed, and the family should be informed that AVMs could still develop despite normal MRI results.
  • Toru Sasamori, Kazutoshi Hida, Shunsuke Yano, Takeshi Asano, Toshitaka Seki, Kiyohiro Houkin
    EUROPEAN SPINE JOURNAL 25 3 748 - 754 2016年03月 [査読有り][通常論文]
     
    To examine the validity of our treatment strategy for spinal dural arteriovenous fistulae (SDAVF), based on the treatment results and the long-term outcome. This study included 50 SDAVF patients (38 men, 12 women, mean age 63.2 years) with progressive myelopathy. The treatment strategy involved embolization as the initial management tool and surgery if embolization was considered unsuitable. Their medical records were evaluated to identify the treatment results and functional outcomes. The mean follow-up period was 81.2 months (range 27-184 months). Complete obliteration was achieved in 22 (71.0 %) of 31 embolized patients and in 18 of 19 (94.7 %) operated patients. The initial success rate was significantly lower in embolized than operated patients. At the last follow-up, 33 of the 50 patients (66 %) manifested improved gait and 16 (32 %) improved micturition. The activity of daily living (ADL) was improved in 33 (66 %). When we compared the rates of functional improvement at the last follow-up, there was no significant difference between patients treated initially by embolization or surgery. The long-term outcomes in SDAVF patients treated by multidisciplinary management with first-line embolization were comparable to those in earlier surgical series. However, our results were unable to demonstrate the superiority of endovascular embolization to surgical treatment for SDAVF. For the purpose of justifying endovascular embolization as a first-line treatment for SDAVF, it will be necessary to show further improvement in both the initial treatment success and the complication rates.
  • Shimoda Yusuke, Moriwaki Takuya, Nakayama Naoki, Abumiya Takeo, Kazumata Ken, Shichinohe Hideo, Houkin Kiyohiro
    STROKE 47 2016年02月 [査読有り][通常論文]
  • Abumiya Takeo, Furukawa Koji, Sakai Keiji, Hirano Miki, Osanai Toshiya, Shichinohe Hideo, Nakayama Naoki, Kazumata Ken, Aida Toshimitsu, Houkin Kiyohiro
    STROKE 47 2016年02月 [査読有り][通常論文]
  • Shichinohe Hideo, Tan Chengbo, Saito Hisayasu, Miyamoto Michiyuki, Hamauchi Shuji, Abumiya Takeo, Nakayama Naoki, Kazumata Ken, Houkin Kiyohiro, Kuroda Satoshi
    STROKE 47 2016年02月 [査読有り][通常論文]
  • Kurisu Kota, Abumiya Takeo, Ito Masaki, Shichinohe Hideo, Nakayama Naoki, Kazumata Ken, Osanai Toshiya, Houkin Kiyohiro
    STROKE 47 2016年02月 [査読有り][通常論文]
  • Satoshi Yamada, Koichi Oki, Yoshiaki Itoh, Satoshi Kuroda, Kiyohiro Houkin, Teiji Tominaga, Susumu Miyamoto, Nobuo Hashimoto, Norihiro Suzuki
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 25 2 340 - 349 2016年02月 [査読有り][通常論文]
     
    Background: Despite the common practice of surgery and antiplatelet therapy for the prevention of recurrent stroke in patients with moyamoya disease, the benefit of these treatments is controversial. We analyzed the stroke recurrence rate in the Registry Study of Research Committee on Moyamoya Disease in Japan funded by the Health, Labor and Welfare Ministry of Japan. Methods: An annual follow-up study of the registered cases was continued for 10 years. The rate of recurrent stroke, including cerebral infarction and hemorrhage but not transient ischemic attack and seizure, was evaluated with Kaplan-Meier analysis. Results: The proportion of childhood-onset cases decreased in recently registered cases (within 10 years, n = 541) compared to remote cases (> 10 years, n = 735). Among types at disease onset in adult-onset cases, intracerebral hemorrhage decreased recently. In recent cases, the rate of subsequent cerebral hemorrhage was much higher in the hemorrhagic group (10.9 +/- 3.3%/5 years) than in the ischemic group (2.0 +/- .9%/5 years). The recurrence rate of cerebral infarction was lower in the surgery group (1.8 +/- .9%/5 years) than in the nonsurgery group (3.8 +/- 2.2%/5 years). In the adult-onset ischemic group, the proportion of surgically treated patients increased and their recurrence rate was lower than that of nonsurgery patients. In the ischemic group, the rate of cerebral infarction was not significantly different between the antiplatelet subgroup and the non-antiplatelet subgroup, whereas the rate of cerebral hemorrhage was higher in the non-antiplatelet subgroup than in the antiplatelet subgroup. Conclusions: Our results suggest revascularization surgery may suppress recurrent ischemic attacks in patients with moyamoya disease. (C) 2015 National Stroke Association. Published by Elsevier Inc. All rights reserved.
  • Kobayashi Satoshi, Osanai Toshiya, Miyamoto Michiyuki, Shimoda Yusuke, Ushikochi Satoshi, Ooka Tomonori, Matsui Yoshiro, Hatanaka Kanako, Houkin Kiyohiro
    Journal of Neuroendovascular Therapy 特定非営利活動法人 日本脳神経血管内治療学会 2016年 
    Objective: Ventricular assist devices (VAD) are used as a bridge to heart transplantation. Cerebral embolism can develop in patients with VADs despite a strict anticoagulation therapy. We report two cases of successful acute endovascular therapy with Penumbra system for cerebral embolism.Case Presentations: Case 1: A 20-year-old woman with a VAD presented with right facial palsy and right monoparesis. 3D-computed tomography angiography (3D-CTA) demonstrated left middle cerebral artery (MCA) occlusion. We successfully performed endovascular recanalization of the left MCA with Thrombolysis in Cerebral Infarction (TICI) Grade3 perfusion, and the patient's symptoms significantly improved. The patient's condition improved to nearly the pre-treatment state. However, due to repeated strokes, the patient developed disturbances of consciousness, aphasia, and complete paralysis of the left lower limb. Furthermore, the infection worsened, and the patient eventually developed a circulatory disorder of the VAD and died. Case 2: A 34-year-old man with VAD presented with sudden disturbances of consciousness and right hemiparesis. Computed tomography (CT) scan revealed no early abnormalities. We performed endovascular recanalization of the left internal carotid artery (IC) top with TICI 3 perfusion. After endovascular treatment, CT scan did not reveal a cerebral infarction. The patient eventually achieved an National Institutes of Health Stroke Scale (NIHSS) score of 0 point and received a heart transplant. In case 2, we pathologically analyzed the retrieved thrombus and confirmed the thrombus to be mainly composed of fibrin. There were no complications of hemorrhage in either case.Conclusion: A suction-type thrombectomy device is believed to be the first choice for patients with VAD.
  • 七戸秀夫, 寳金清博
    脳循環代謝(Web) 28 1 2016年
  • 寳金清博
    レギュラトリーサイエンス学会誌 6 Supplement 2016年
  • Chengbo Tan, Hideo Shichinohe, Zifeng Wang, Shuji Hamauchi, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Tsuneo Ito, Kohsuke Kudo, Shigeru Takamoto, Kiyohiro Houkin
    Stem Cells International 2016 2016年 [査読有り][通常論文]
     
    Currently, there is increasing interest in human bone marrow stromal cells (hBMSCs) as regeneration therapy against cerebral stroke. The aim of the present study was to evaluate the feasibility and validity of hBMSC cultures with allogeneic platelet lysates (PLs). Platelet concentrates (PC) were harvested from healthy volunteers and made into single donor-derived PL (sPL). The PL mixtures (mPL) were made from three different sPL. Some growth factors and platelet cell surface antigens were detected by enzyme-linked immunosorbent assay (ELISA). The hBMSCs cultured with 10% PL were analyzed for their proliferative potential, surface markers, and karyotypes. The cells were incubated with superparamagnetic iron oxide (SPIO) agents and injected into a pig brain. MRI and histological analysis were performed. Consequently, nine lots of sPL and three mPL were prepared. ELISA analysis showed that PL contained adequate growth factors and a particle of platelet surface antigens. Cell proliferation capacity of PLs was equivalent to or higher than that of fetal calf serum (FCS). No contradiction in cell surface markers and no chromosomal aberrations were found. The MRI detected the distribution of SPIO-labeled hBMSCs in the pig brain. In summary, the hBMSCs cultured with allogeneic PL are suitable for cell therapy against stroke.
  • Uchino H, Motegi H, Kobayashi H, Kamoshima Y, Kazumata K, Terasaka S, Houkin K
    NMC case report journal 3 1 1 - 4 2016年01月 [査読有り][通常論文]
     
    This article details two case reports and reviews the extant literature concerning acute postoperative submandibular sialadenitis occurring in the side contralateral to the operated site after neurosurgery. Although its precise pathogenesis remains to be elucidated, it is likely related to intraoperative compression of the submandibular gland and surrounding tissues, caused by head positioning and the endotracheal tube. Submandibular swelling rapidly deteriorates after surgery, and emergent airway protection is required in most cases in order to avoid fatal airway obstruction. To avoid serious sequelae, we should be aware of acute submandibular sialadenitis that occurs contralateral to the surgical side, which, although rare, is more probable after posterior fossa surgery. When it occurs, early airway protection is crucial, and the following conservative treatment could provide a good prognosis.
  • Toru Sasamori, Kazutoshi Hida, Toshiya Osanai, Shunsuke Yano, Toshitaka Seki, Kiyohiro Houkin
    NEUROSURGERY 78 1 E162 - E162 2016年01月 [査読有り][通常論文]
  • Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Shigeru Yamaguchi, Yukitomo Ishi, Yasuhiro Ito, Kiyohiro Houkin
    ACTA NEUROCHIRURGICA 158 1 87 - 92 2016年01月 [査読有り][通常論文]
     
    Anatomical landmarks such as choroid plexus and foramen of Monro are very important to undergo intraventricular surgery safely and effectually. These landmarks would be unclear in cases with a huge cyst or repeat surgery. We report the usability and precautions to apply a bronchoscope with an ultrasonic convex probe to intraventricular surgery. Two patients diagnosed with obstructive hydrocephalus, one with a large cyst and the other with recurrent craniopharyngioma in the third ventricle, were applied to the EBUS system. In both patients, the EBUS system was applied safely, and lesions beyond the wall of ventricles or the cyst were visible. Color Doppler ultrasonography detected choroid plexus and internal cerebral veins. Furthermore, we performed real-time ultrasound-guided cyst puncture safely on the case with a large cyst. The most important precaution is that the curved portion of the EBUS system is too long to be bent within cerebral ventricles. The new EBUS system with an ultrasonic convex probe is a novel and effectual device to perform intraventricular surgery.
  • Kota Kurisu, Toshiya Osanai, Ken Kazumata, Naoki Nakayama, Takeo Abumiya, Hideo Shichinohe, Yusuke Shimoda, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 56 12 745 - 752 2016年 [査読有り][通常論文]
     
    Although ultrasound (US) guidance for venous access is becoming the "standard of care" for preventing access site complications, its feasibility for arterial access has not been fully investigated, especially in the neuro-interventional population. We conducted the first prospective cohort study on US-guided femoral artery access during neuro-interventional procedure. This study included 64 consecutive patients who underwent US-guided femoral artery access through 66 arterial access sites for diagnostic and/or neurointerventional purposes. The number of attempts required for both the sheath insertion and the success of anterior wall puncture were recorded. In addition, the occurrence of major complications and hematoma formation on the arterial access site examined by US were statistically analyzed. The median number of attempts was 1 (1-2) and first-pass success rate was 63.6%. Anterior wall puncture was achieved in 98.5%. In one case (1.5%), a pseudoaneurysm was observed. In all cases, US clearly depicted a common femoral artery (CFA) and its bifurcation. Post-procedural hematoma was detected in 13 cases (19.7%), most of which were "tiny" or "moderate" in size. Low body mass index and antiplatelet therapy were the independent risk factors for access site hematoma. The US-guided CFA access was feasible even in neuro-interventional procedure. The method was particularly helpful in the patients with un-palpable pulsation of femoral arteries. To prevent arterial access site hematoma, special care should be taken in patients with low body mass index and who are on antiplatelet therapy.
  • Hideo Shichinohe, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 56 10 592 - 596 2016年 [査読有り][通常論文]
     
    Stroke is still a leading cause of death and disability, and despite intensive research, few treatment options exist. A recent breakthrough in cell therapy is expected to reverse the neurological sequelae of stroke. Although some pioneer studies on the use of cell therapy for the treatment of stroke have been reported, certain problems still remain unsolved. We investigated the use of autologous bone marrow stromal cell (BMSC) transplantation for the treatment of stroke, to develop it as the next-generation cell therapy. In this study, we introduce the preparation of a new clinical trial, the Research on Advanced Intervention using Novel Bone marrow stem cell (RAINBOW) study. The trial will start in 2016, and we hope that it will not only be helpful for treating patients but also for clarifying the therapeutic mechanisms. Moreover, we review stem cell therapeutics as an emerging paradigm in stroke (STEPS) and the guidelines for the development of cell therapy for stroke in the United States as well as introduce the development of new guidelines in Japan. These guidelines are expected to encourage the development of cell therapy for stroke management.
  • Toshitaka Seki, Kazutoshi Hida, Shunsuke Yano, Takeshi Aoyama, Izumi Koyanagi, Toru Sasamori, Shuji Hamauch, Kiyohiro Houkin
    Asian Spine Journal 10 4 748 - 754 2016年 [査読有り][通常論文]
     
    Study Design: Retrospective study. Purpose: To obtain information useful in establishing treatment guidelines by evaluating baseline clinical features and treatment outcomes of patients with spinal cord astrocytoma (SCA). Overview of Literature: The optimal management of SCA remains controversial, and there are no standard guidelines. Methods: The study included 20 patients with low-grade and 13 with high-grade SCA surgically treated between 1989 and 2014. Patients were classified according to the extent of surgical resection. Survival was assessed using Kaplan-Meier plots and compared between groups by log-rank tests. Neurological status was defined by the modified McCormick scale and compared between groups by Mann-Whitney U tests. Results: Surgical resection was performed for 19 of 20 low-grade (95%) and 10 of 13 high-grade (76.9%) SCA patients. Only nine patients (27.3%) underwent gross total resection, all of whom had low-grade SCA. Of all patients, 51.5% showed deteriorated neurological status compared to preoperative baseline. Median overall survival was significantly longer for low-grade SCA than that for high-grade SCA (91 months, 78% at 5 years vs. 15 months, 31% at 5 years p =0.007). Low-grade SCA patients benefited from more aggressive resection, whereas high-grade SCA patients did not. Multivariate analysis revealed histology status (hazard ratio [HR], 0.30 95% confidence interval [CI], 0.09-0.98 p < 0.05) and postoperative neurological status (HR, 0.12 CI, 0.02-0.95 p < 0.05) as independent predictors of longer overall survival. Adjuvant radiotherapy had no significant impact on survival rate. However, a trend for increased survival was observed with radiation cordotomy (RCT) in high-grade SCA patients. Conclusions: Aggressive resection for low-grade and RCT for high-grade SCA may prolong survival. Preservation of neurological status is an important treatment goal. Given the low incidence of SCA, establishing strong collaborative, interdisciplinary, and multiinstitutional study groups is necessary to define optimal treatments.
  • Masaki Ito, Yoshimasa Niiya, Masashi Kojima, Hiroyuki Itosaka, Motoyuki Iwasaki, Ken Kazumata, Shoji Mabuchi, Kiyohiro Houkin
    Acta Neurochirurgica, Supplementum 123 115 - 122 2016年 [査読有り][通常論文]
     
    Background: External carotid artery (ECA) positioned laterally to the internal carotid artery (ICA) at the level of the common carotid artery (CCA) bifurcation is occasionally encountered during carotid endarterectomy (CEA). This study aimed to determine the frequency of this phenomenon and provide technical tips for performing CEA. Methods: The study included 199 consecutive patients (209 carotid arteries) who underwent CEA at Otaru Municipal Medical Center in 2007–2014. The position of the ECA with respect to the ICA at the CCA bifurcation was preoperatively rated as either lateral or normal, using three-dimensional computerized tomographic angiography (3-D CTA) anteroposterior projections. Postoperative diffusion-weighted images (DWIs), and postoperative 3-D CTA images were reviewed. Results: Among the 209 carotid arteries with atherosclerosis, 11 instances (5.3%) of lateral position of the ECA were detected in 11 patients. Ten of these arteries (91%) were right-sided (odds ratio 11.1 95% confidence interval 1.38–88.9). Wider longitudinal exposure of the arteries was used during CEA, and the CCA and ECA were rotated clockwise or counter clockwise. The ICA lying behind the ECA along the surgical access route was then pulled out laterally and moved to the shallow surgical field. Cross-clamping, arteriotomy, plaque removal, and wall suturing were performed as usual. No cerebral infarcts were detected on postoperative DWIs, and 3-D CTA revealed no CCA and ICA kinking. Conclusions: Lateral position of the ECA is not extremely rare in patients undergoing CEA for atherosclerosis and may be a congenital variation, although this is still controversial. CEA can be performed safely if the arteries from the CCA to the ICA are rotated, and the ICA is moved to the shallow surgical field under wider longitudinal exposure. Although no postoperative cerebral infarcts were detected, the risk of artery-toartery embolism resulting from artery repositioning prior to plaque removal should be taken into consideration.
  • Yukitomo Ishi, Hiroyuki Kobayashi, Hiroaki Motegi, Shogo Endo, Shigeru Yamaguchi, Shunsuke Terasaka, Kiyohiro Houkin
    NEUROLOGIA I NEUROCHIRURGIA POLSKA 50 6 504 - 510 2016年 [査読有り][通常論文]
     
    Background: Compared with surgical resection, endoscopic transsphenoidal surgery (TSS) for cholesterol granuloma (CG) in the petrous apex (PA) is associated with local recurrence due to obstruction of the drainage route. We present a detailed procedure of an endoscopic TSS using pedicle vascularized nasoseptal flap (PVNF). Methods: A 40-year-old woman with a history of repeated surgery for left tympanitis was referred to our institution. Neurological examination revealed severe hearing loss in the left ear. Radiologic examination presented a round mass in the left PA and significant fluid collection in the mastoid air cells of the left temporal bone. CG was strongly suspected, and endoscopic TSS using PVNF was performed. Prior to endoscopic drainage, a PVNF was harvested from the mucosa of the ipsilateral nasal septum, with an attempt to preserve the sphenopalatine artery in the flap. Following this, puncture and adequate irrigation of the lesion was performed by endoscopic TSS, with neuro-navigation system assistance; the apex of PVNF was then placed into the lesion to prevent the obstruction of the drainage route. An absorbable polyglycolic acid sheet and fibrin glue were applied on the flap to prevent spontaneous deviation from the lesion. Results: The patient was discharged without any further neurological complications. Eight month postoperative computed tomography images showed no recurrence; the drainage route was patent and the fluid collection in the left mastoid air cells was resolved. Moreover, hearing loss was improved. Conclusions: Endoscopic TSS using PVNF may be one of available surgical options for PACG. (C) 2016 Polish Neurological Society. Published by Elsevier Sp. z o.o. All rights reserved.
  • Toshitaka Seki, Kazutoshi Hida, Shunsuke Yano, Toru Sasamori, Shuji Hamauch, Izumi Koyanagi, Kiyohiro Houkin
    Asian Spine Journal 10 5 940 - 944 2016年 [査読有り][通常論文]
     
    Study Design: Retrospective cohort study. Purpose: To compare long-term results of surgery with the outcomes of symptomatic and asymptomatic tethered cord syndrome (TCS) in children and adolescents and to assess the surgery duration for those with TCS. Overview of Literature: Pediatric patients with TCS continue to pose significant diagnostic and management challenges. Methods: We retrospectively analyzed the outcomes of 31 patients (16 males, 15 females) with TCS, including 21 with lumbosacral lipoma. All were surgically treated between 1989 and 2015. They were divided into symptomatic and asymptomatic TCS groups. The results of the treatment were summarized and analyzed using a non-parametric Mann-Whitney U test. Results: Nineteen patients had symptomatic TCS and 12 had asymptomatic TCS. Patients had a median age of 34 months (range, 0-201 months). The median follow-up period was 116 months (range, 7-223 months). Of the 19 symptomatic TCS patients, preoperative deficits improved after surgery in two (10.5%) and remained stable in 17 (89.5%) patients. One of the 12 asymptomatic TCS (8.3%) patients showed an exacerbated illness after surgery, and one in 11 patients remained stable (11.7%). There were significant differences in monthly age at surgery, preoperative bowel and bladder dysfunction, neurological function, presence/absence of clean intermittent catheterization introduction, and presence/absence of motor disorder at final follow-up (all p < 0.05). In the univariate analysis, the presence/absence of preoperative bowel and bladder dysfunction, and symptoms were strongly associated with the risk of children and adolescents with TCS (p < 0.05). Conclusions: Early accurate diagnosis and adequate surgical release might lead to successful outcomes in children and adolescents with TCS. Surgical untethering is a safe and effective method for treatment of children with TCS.
  • Chengbo Tan, Hideo Shichinohe, Zifeng Wang, Shuji Hamauchi, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Tsuneo Ito, Kohsuke Kudo, Shigeru Takamoto, Kiyohiro Houkin
    STEM CELLS INTERNATIONAL 2016 6104780 - 6104780 2016年 [査読有り][通常論文]
     
    Currently, there is increasing interest in human bone marrow stromal cells (hBMSCs) as regeneration therapy against cerebral stroke. The aim of the present study was to evaluate the feasibility and validity of hBMSC cultures with allogeneic platelet lysates (PLs). Platelet concentrates (PC) were harvested from healthy volunteers and made into single donor-derived PL (sPL). The PL mixtures (mPL) were made from three different sPL. Some growth factors and platelet cell surface antigens were detected by enzyme-linked immunosorbent assay (ELISA). The hBMSCs cultured with 10% PL were analyzed for their proliferative potential, surface markers, and karyotypes. The cells were incubated with superparamagnetic iron oxide (SPIO) agents and injected into a pig brain. MRI and histological analysis were performed. Consequently, nine lots of sPL and three mPL were prepared. ELISA analysis showed that PL contained adequate growth factors and a particle of platelet surface antigens. Cell proliferation capacity of PLs was equivalent to or higher than that of fetal calf serum (FCS). No contradiction in cell surface markers and no chromosomal aberrations were found. The MRI detected the distribution of SPIO-labeled hBMSCs in the pig brain. In summary, the hBMSCs cultured with allogeneic PL are suitable for cell therapy against stroke.
  • Jun C. Takahashi, Takeshi Funaki, Kiyohiro Houkin, Tooru Inoue, Kuniaki Ogasawara, Jyoji Nakagawara, Satoshi Kuroda, Keisuke Yamada, Susumu Miyamoto
    STROKE 47 1 37 - 43 2016年01月 [査読有り][通常論文]
     
    Background and Purpose The primary results of the Japan Adult Moyamoya Trial revealed the statistically marginal superiority of bypass surgery over medical treatment alone in preventing rebleeding in moyamoya disease. The purpose of this analysis is to test the prespecified subgroup hypothesis that the natural course and surgical effects vary depending on the hemorrhagic site at onset. Methods The hemorrhagic site, classified as either anterior or posterior, was the only stratifying variable for randomization. Statistical analyses were focused on the assessment of effect modification according to the hemorrhagic site and were based on tests of interaction. Results Of 42 surgically treated patients, 24 were classified as anterior hemorrhage and 18 as posterior hemorrhage; of 38 medically treated patients, 21 were classified as anterior and 17 as posterior. The hazard ratio of the primary end points (all adverse events) for the surgical group relative to the nonsurgical group was 0.07 (95% confidence interval, 0.01-0.55) for the posterior group, as compared with 1.62 (95% confidence interval, 0.39-6.79) for the anterior group (P=0.013 for interaction). Analysis within the nonsurgical group revealed that the incidence of the primary end point was significantly higher in the posterior group than in the anterior group (17.1% per year versus 3.0% per year; hazard ratio, 5.83; 95% confidence interval, 1.60-21.27). Conclusions Careful interpretation of the results suggests that patients with posterior hemorrhage are at higher risk of rebleeding and accrue greater benefit from surgery, subject to verification in further studies.
  • 高宮宗一朗, 長内俊也, 牛越聡, 栗栖宏多, 下田祐介, 伊藤康裕, 伊師雪友, 穂刈正昭, 中山若樹, 数又研, 鐙谷武雄, 七戸秀夫, 寳金清博
    Neurological Surgery 44 1 39 - 45 2016年01月 [査読有り][通常論文]
     
    Systemic vascular diseases such as fibromuscular dysplasia, Ehlers-Danlos syndrome, Marfan syndrome, and Behçet's disease are known to cause spontaneous dissecting aneurysms of the cervical internal carotid artery. These diseases are generally associated with vascular fragility; therefore, invasive treatments are avoided in many cases of dissecting aneurysms, and a conservative approach is used for the primary disease. Surgical or intravascular treatment may be chosen when aneurysms are progressive or are associated with a high risk of hemorrhage; however, there is no consensus on which treatment is better. We report a case of a dissecting aneurysm of the cervical internal carotid artery in a patient with suspected Behçet's disease, which was treated using stent-assisted coil embolization. A man in his 40's, with suspected Behçet's disease, presented with an enlarged dissecting aneurysm of the right cervical internal carotid artery. The lesion was present for approximately 10 years. We performed stent-assisted coil embolization for the lesion. Post-surgery, no aneurysms were detected with carotid artery echography. Our case report suggests that stent-assisted coil embolization is a promising treatment for dissecting aneurysms of the cervical internal carotid artery. In addition, the procedure demonstrates the utility of carotid artery echograms for examining recanalization after stent-assisted coil embolization.
  • Taku Sugiyama, Ken Kazumata, Katsuyuki Asaoka, Toshiya Osanai, Daisuke Shimbo, Kazuki Uchida, Yuka Yokoyama, Naoki Nakayama, Kouji Itamoto, Kiyohiro Houkin
    World neurosurgery 84 6 1579 - 88 2015年12月 [査読有り][通常論文]
     
    BACKGROUND: Intravenous thrombolysis using tissue plasminogen activator and endovascular treatment for acute ischemic stroke is becoming an established standard therapy. However, there is no consensus in the treatment of patients who are suffering from progressive neurologic symptoms in the later stages. The purpose of this study was to evaluate the safety and efficacy of microsurgical revascularization in such patients with progressive stroke. METHODS: We retrospectively reviewed the clinical and radiological records of 14 consecutive patients with progressive stroke who underwent emergency open surgery for anterior circulation occlusion within 7 days after onset. Surgical candidates were carefully selected on the basis of symptom severity, diffusion-weighted imaging, and perfusion study. Superficial temporal artery to middle cerebral artery bypass was applied for atherosclerotic occlusion, and microsurgical embolectomy was applied for embolic occlusion. RESULTS: Superficial temporal artery to middle cerebral artery bypass was performed in 12 patients, microsurgical embolectomy in 1, and the combination of these modalities in 1. As a result, complete revascularization was achieved in all patients. The National Institutes of Health Stroke Scale scores significantly improved after surgery (at third postoperative day, P < 0.05; at 14th postoperative day, P < 0.01). A favorable outcome (modified Rankin Scale 0-2) was achieved in 12 of the 14 (85.7%) patients. Minor intracerebral hemorrhage occurred in 1 patient and hyperperfusion syndrome occurred in 1 patients; however, the patients subsequently recovered without additional treatment. CONCLUSIONS: Microsurgical revascularization is a feasible treatment option for patients with progressive stroke due to anterior circulation major vessel occlusion.
  • 栗栖 宏多, 長内 俊也, 飛彈 一利, 浅野 剛, 牛越 聡, 関 俊隆, 笹森 徹, 寶金 清博
    JNET: Journal of Neuroendovascular Therapy 9 6 S316 - S316 (NPO)日本脳神経血管内治療学会 2015年11月
  • Tomohito Hishikawa, Isao Date, Koji Tokunaga, Shinjiro Tominari, Kazuhiko Nozaki, Yoshiaki Shiokawa, Kiyohiro Houkin, Yuichi Murayama, Toshihiro Ishibashi, Hiroyuki Takao, Toshikazu Kimura, Takeo Nakayama, Akio Morita
    NEUROLOGY 85 21 1879 - 1885 2015年11月 [査読有り][通常論文]
     
    Objectives:The aim of this study was to identify risk factors for rupture of unruptured cerebral aneurysms (UCAs) in elderly Japanese patients aged 70 years or older.Methods:The participants included all patients 70 years of age or older in 3 prospective studies in Japan (the Unruptured Cerebral Aneurysm Study of Japan [UCAS Japan], UCAS II, and the prospective study at the Jikei University School of Medicine). A total of 1,896 patients aged 70 years or older with 2,227 UCAs were investigated. The median and mean follow-up periods were 990 and 802.7 days, respectively.Results:The mean aneurysm size was 6.2 3.9 mm. Sixty-eight patients (3.6%) experienced subarachnoid hemorrhage during the follow-up period. Multivariable analysis per patient revealed that in patients aged 80 years or older (hazard ratio [HR], 2.02; 95% confidence interval [CI], 1.16-3.49, p = 0.012), aneurysms 7 mm or larger (HR, 3.08; 95% CI, 1.35-7.03, p = 0.007 for 7-9 mm; HR, 7.82; 95% CI, 3.60-16.98, p < 0.001 for 10-24 mm; and HR, 43.31; 95% CI, 12.55-149.42, p < 0.001 for 25 mm) and internal carotid-posterior communicating artery aneurysms (HR, 2.45; 95% CI, 1.23-4.88, p = 0.011) were independent predictors for UCA rupture in elderly patients.Conclusions:In our pooled analysis of prospective cohorts in Japan, patient age and aneurysm size and location were significant risk factors for UCA rupture in elderly patients.
  • キャンサーボードが機能した1症例
    遠藤 将吾, 寺坂 俊介, 小林 浩之, 山口 秀, 茂木 洋晃, 金子 貞洋, 井口 晶裕, 長 祐子, 大島 淳二郎, 寺坂 友佳代, 杉山 未奈子, 橋本 孝之, 鬼丸 力也, 清水 伸一, 有賀 正, 白戸 博樹, 寳金 清博
    日本小児血液・がん学会雑誌 52 3 343 - 343 (NPO)日本小児血液・がん学会 2015年10月 [査読無し][通常論文]
  • Haruto Uchino, Masaki Ito, Noriyuki Fujima, Ken Kazumata, Kazuyoshi Yamazaki, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
    CLINICAL NEUROLOGY AND NEUROSURGERY 137 105 - 111 2015年10月 [査読有り][通常論文]
     
    Background: Noncontrast-enhanced time-resolved four-dimensional magnetic resonance angiography using an arterial spin labeling technique (ASL-4D MRA) is emerging as a next generation angiography for the management of neurovascular diseases. This study evaluated the feasibility of ASL-4D MRA for the diagnosis of Moyamoya disease (MMD) and MMD staging by using digital subtraction angiography (DSA) and time-of-flight MRA (TOF MRA) as current standards. Methods: Eleven consecutive non-operated patients who underwent DSA for the diagnosis of MMD were recruited. Two independent observers evaluated the three tests. The data were analyzed for inter-observer and inter-modality agreements on MMD stage. Nine of 22 hemispheres underwent surgical revascularization and ASL-4D MRA was repeated postoperatively. Results: Time-resolved inflow of blood through the cerebral vessels, including moyamoya vessels, was visualized in all the 22 non-operated hemispheres. MMD stages assessed by DSA and ASL-4D MRA were completely matched in 18 hemispheres, with a significant positive correlation between these modalities (r=0.93, P<0.001). Inter-observer agreement with ASL-4D MRA (K=0.91 +/- 0.04, P<0.001) and inter-modality agreement between ASL-4D MRA and DSA (K = 0.93 +/- 0.04, P<0.001) were both excellent. MMD stages assessed by ASL-4D MRA have also a significant positive correlation with those assessed by TOF MRA (r=0.68, P=0.004). Repeated ASL-4D MRA clearly demonstrated the bypassed arteries and changes in the dynamic flow patterns of cerebral arteries in all the nine hemispheres after surgical revascularization. Of these, postoperative focal hyperperfusion was detected by single photon emission tomography in 7 hemispheres. In five of the seven hemispheres (71%) with postoperative hyperperfusion, ASL-4D MRA demonstrated focal hyperintense signals in the bypassed arteries, although TOF MRA did not. Conclusions: Noninvasive ASL-4D MRA is feasible for the diagnosis of MMD staging. This next generation angiography may be useful for monitoring disease evolution and treatment response in cerebral arteries after revascularization surgery in MMD. (C) 2015 Elsevier B.V. All rights reserved.
  • Sadahiro Kaneko, Yuka Nakatani, Tatsuya Takezaki, Takuichiro Hide, Daisuke Yamashita, Naoki Ohtsu, Takanori Ohnishi, Shunsuke Terasaka, Kiyohiro Houkin, Toru Kondo
    CANCER RESEARCH 75 19 4224 - 4234 2015年10月 [査読有り][通常論文]
     
    Glioblastoma-initiating cells (GIC) are a tumorigenic cell subpopulation resistant to radiotherapy and chemotherapy, and are a likely source of recurrence. However, the basis through which GICs are maintained has yet to be elucidated in detail. We herein demonstrated that the carcinoembryonic antigen-related cell adhesion molecule Ceacam1L acts as a crucial factor in GIC maintenance and tumorigenesis by activating c-Src/STAT3 signaling. Furthermore, we showed that monomers of the cytoplasmic domain of Ceacam1L bound to c-Src and STAT3 and induced their phosphorylation, whereas oligomerization of this domain ablated this function. Our results suggest that Ceacam1L-dependent adhesion between GIC and surrounding cells play an essential role in GIC maintenance and proliferation, as mediated by signals transmitted by monomeric forms of the Ceacam1L cytoplasmic domain. (C) 2015 AACR.
  • Yoshifumi Horita, Takeshi Mikami, Kiyohiro Houkin, Nobuhiro Mikuni
    Surgical Neurology International 6 Suppl 9 S291 - S294 2015年09月01日 [査読有り][通常論文]
     
    Background: Dilative arteriopathy is a form of dolichoectasia. It is sometimes observed in the posterior circulation, and it may be associated with various type of stroke. Herein, we report two unusual cases of saccular aneurysms associated with a segmental dilative arteriopathy located in the anterior circulation. Case Descriptions: The first case is a 39-year-old woman with irregular tortuosity and coiling of the left internal cerebral artery along with saccular aneurysms in this artery. The second case is a 45-year-old woman presenting with a ruptured saccular aneurysm in the coiling of the anterior cerebral artery. In both cases, the aneurysm was clipped successfully, and the patients recovered uneventfully with no neurological deficits. Conclusion: Dilative arteriopathy of the circle of Willis is an unusual anomaly and is characterized by tortuous and elongated arteries, which are sometimes observed in patients with a congenital anomaly. This report describes two cases of saccular aneurysm associated with dilative arteriopathy of the circle of Willis with no medical history, which to the best of our knowledge has not previously been described.
  • Endo S, Kudo N, Yamaguchi S, Sumiyoshi K, Motegi H, Kobayashi H, Terasaka S, Houkin K
    Ultrasound in medicine & biology 41 9 2458 - 2465 2015年09月 [査読有り][通常論文]
     
    Because it is highly infiltrative, malignant glioma is a cancer with a poor prognosis despite multidisciplinary treatment strategies, such as aggressive surgery and chemoradiotherapy, necessitating new therapeutic approaches to control migration of tumor cells. In our study, we investigated the efficacy of sonodynamic therapy of glioma cells in vitro using porphyrin derivatives, including 5-aminolevulinic acid, protoporphyrin IX and talaporfin sodium, as sonosensitizers. These substances have been known to accumulate in glioma cells and are expected to have cytotoxic effects on sonication. Our study found that the cytotoxicity of sonication of glioma cells is enhanced by each sonosensitizer and that the efficacy of sonodynamic therapy may depend on the degree of intracellular accumulation of sonosensitizer. Also, the study suggests that induction of apoptosis is a major mechanism underlying cell death. Though further investigations are necessary, our preliminary result indicates a potential for sonodynamic therapy with sonosensitizers in glioma treatment. (C) 2015 World Federation for Ultrasound in Medicine & Biology.
  • Shimoda Yusuke, Nakayama Naoki, Hokari Masaaki, Abumiya Takeo, Shichinohe Hideo, Kazumata Ken, Houkin Kiyohiro
    JOURNAL OF NEUROSURGERY 123 2 A526  2015年08月 [査読有り][通常論文]
  • Masaaki Hokari, Naoki Nakayama, Ken Kazumata, Toshiya Osanai, Toshitaka Nakamura, Hiroshi Yasuda, Satoshi Ushikoshi, Hideo Shichinohe, Takeo Abumiya, Satoshi Kuroda, Kiyohiro Houkin
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 24 8 1768 - 74 2015年08月 [査読有り][通常論文]
     
    BACKGROUND: Carotid endarterectomy (CEA) and carotid stenting (CAS) are beneficial procedures for patients with high-grade cervical carotid stenosis. However, it is sometimes difficult to manage patients with bilateral carotid stenosis. To decide the treatment strategy, one of the most important questions is whether contralateral stenosis increases the risk of patients undergoing CEA. METHODS: This retrospective study included 201 patients with carotid stenosis who underwent a total of 219 consecutive procedures (CEA 189/CAS 30). We retrospectively analyzed outcomes in patients with carotid stenosis who were treated with either CEA or CAS and evaluated whether or not contralateral lesions increases the risk of patients undergoing CEA or CAS. Furthermore, we retrospectively verified our treatment strategy for bilateral carotid stenosis. RESULTS: The incidences of perioperative complications were 5.3% in the CEA patients and 6.7% in the CAS patients, respectively. There was no significant difference between these 2 groups. The existences of contralateral occlusion and/or contralateral stenosis were not associated with perioperative complications in both the groups. There were 32 patients with bilateral severe carotid stenosis (>50%). Of those, 13 patients underwent bilateral revascularizations; CEA followed by CEA in 8, CEA followed by CAS in 3, CAS followed by CEA + coronary artery bpass grafting in 1, and CAS followed by CAS in 1. CONCLUSIONS: Our date showed that the existence of contralateral carotid lesion was not associated with perioperative complications, and most of our cases with bilateral carotid stenosis initially underwent CEA.
  • Michiharu Yoshida, Shigeru Yamaguchi, Yukitomo Ishi, Shogo Endo, Hiroaki Motegi, Hiroyuki Kobayashi, Katsuyuki Asaoka, Yuuta Kamoshima, Shunsuke Terasaka, Kiyohiro Houkin
    Neurological Surgery 43 7 603 - 610 2015年07月01日 [査読有り][通常論文]
     
    Background: In Japan, patients with malignant glioma have been treated with BCNU wafers (Gliadel®) since January 2013. Several adverse events (AEs) associated with implantation of BCNU wafers, including cerebral edema or cyst formation, are recognized. Here, we report a retrospective review of the experience with implantation of BCNU wafers in our institutions and our findings regarding the risk factors for the AEs. Methods: We reviewed the records of patients with malignant glioma who were implanted with BCNU wafers between April 2013 and September 2014. Their AEs were examined clinically and radiologically and evaluated according to Common Terminology Criteria for Adverse Events (CTCAE) grading. For investigating the association between risk factors and incidence of AEs, histological diagnosis, extent of resection, and period of BCNU wafers implantation surgery were selected as possible risk factors. Results: Twenty-one patients were included in this investigation. There were no associations among incidence of AEs and histological diagnosis or extent of tumor resection. However, regarding the period of BCNU wafers implantation, additional resection for newly diagnosed tumors and resection for recurrent tumors tended to increase the rate and severity of AEs, especially cerebral edema, compared to primary resection Conclusion: In cases of BCNU wafers implantation, the incidence and degree of AEs might increase if additional resection for newly diagnosed tumors or resection for recurrent tumors is performed. Our investigation revealed that AEs associated with implantation of BCNU wafers tend to occur in the repeated glioma surgery.
  • Toru Sasamori, Kazutoshi Hida, Toshiya Osanai, Shunsuke Yano, Toshitaka Seki, Kiyohiro Houkin
    Neurosurgery 77 1 113 - 8 2015年07月 [査読有り][通常論文]
     
    BACKGROUND: Chronic pain due to spinal dural arteriovenous fistulae (SDAVF) during follow-up is a serious issue because it can affect patients' quality of life. The severity of posttreatment chronic leg pain in patients with SDAVF is unclear. OBJECTIVE: To investigate the prevalence, severity, and characteristics of chronic leg pain in patients with SDAVF and to examine the clinical factors associated with chronic pain. METHODS: We conducted a survey of their chronic posttreatment leg pain in 49 SDAVF patients. It consisted of a numeric rating scale and a neuropathic pain symptom inventory (NPSI). Of the 49 patients, 32 (65.3%) completed the questionnaire. There were 24 males and 8 females whose mean age was 69.1 years. The mean follow-up period was 86.3 months. RESULTS: Chronic leg pain was reported by 26 (81.3%) of 32 patients; 14 experienced pain before treatment and 12 had new-onset posttreatment pain. The pain was reported as moderate to severe by 21 (80.8%) of 26 patients; the mean numeric rating scale and NPSI scores were 6.0 ± 2.3 and 17.4 ± 10.3, respectively. The NPSI subscores were significantly higher for spontaneous pain and paresthesia/dysesthesia than for paroxysmal pain. We found no significant clinical factors related to chronic posttreatment pain; on magnetic resonance imaging scans we identified spinal cord atrophy in 8 patients with chronic pain. CONCLUSION: Most of our SDAVF patients reported moderate to severe chronic leg pain characterized by spontaneous pain and paresthesia/dysesthesia. Spinal cord atrophy on magnetic resonance imaging scans was a characteristic in patients with chronic pain.
  • Masaaki Hokari, Naoki Nakayama, Hiroshi Nishihara, Kiyohiro Houkin
    NEUROSURGICAL REVIEW 38 3 531 - 540 2015年07月 [査読有り][通常論文]
     
    Although several studies have suggested that aneurysmal wall inflammation and laminar thrombus are associated with the rupture of saccular aneurysms, the mechanisms leading to the rupture remain obscure. We performed full exposure of aneurysms before clip application and attempted to keep the fibrin cap on the rupture point. Using these specimens in a nearly original state before surgery, we conducted a pathological analysis and studied the differences between ruptured and unruptured aneurysms to clarify the mechanism of aneurysmal wall degeneration. This study included ruptured (n=28) and unruptured (n=12) saccular aneurysms resected after clipping. All of the ruptured aneurysms were obtained within 24 h of onset. Immunostainings for markers of inflammatory cells (CD68) and classical histological staining techniques were performed. Clinical variables and pathological findings from ruptured and unruptured aneurysms were compared. Patients with ruptured or unruptured aneurysms did not differ by age, gender, size, location, and risk factors, such as hypertension, smoking, and hyperlipidemia. The absence or fragmentation of the internal elastica lamina, the myointimal hyperplasia, and the thinning of the aneurysmal wall were generally observed in both aneurysms. The existence of subintimal fibrin deposition, organized laminar thrombus, intramural hemorrhage, neovascularization, and monocyte infiltration are more frequently observed in ruptured aneurysms. Multivariate logistic regression analysis showed that ruptured aneurysm was associated with presence of subintimal fibrin deposition and monocyte infiltration. These findings suggest that subintimal fibrin deposition and chronic inflammation have a strong impact on degeneration of the aneurysmal wall leading to their rupture, and this finding may be caused by endothelial dysfunction.
  • Shinjiro Tominari, Akio Morita, Toshihiro Ishibashi, Tomosato Yamazaki, Hiroyuki Takao, Yuichi Murayama, Makoto Sonobe, Masahiro Yonekura, Nobuhito Saito, Yoshiaki Shiokawa, Isao Date, Teiji Tominaga, Kazuhiko Nozaki, Kiyohiro Houkin, Susumu Miyamoto, Takaaki Kirino, Kazuo Hashi, Takeo Nakayama
    ANNALS OF NEUROLOGY 77 6 1050 - 1059 2015年06月 [査読有り][通常論文]
     
    ObjectiveTo build a prediction model that estimates the 3-year rupture risk of unruptured saccular cerebral aneurysms. MethodsSurvival analysis was done using each aneurysm as the unit for analysis. Derivation data were from the Unruptured Cerebral Aneurysm Study (UCAS) in Japan. It consists of patients with unruptured cerebral aneurysms enrolled between 2000 and 2004 at neurosurgical departments at tertiary care hospitals in Japan. The model was presented as a scoring system, and aneurysms were classified into 4 risk grades by predicted 3-year rupture risk: I,<1%; II, 1 to 3%; III, 3 to 9%, and IV, >9%. The discrimination property and calibration plot of the model were evaluated with external validation data. They were a combination of 3 Japanese cohort studies: UCAS II, the Small Unruptured Intracranial Aneurysm Verification study, and the study at Jikei University School of Medicine. ResultsThe derivation data include 6,606 unruptured cerebral aneurysms in 5,651 patients. During the 11,482 aneurysm-year follow-up period, 107 ruptures were observed. The predictors chosen for the scoring system were patient age, sex, and hypertension, along with aneurysm size, location, and the presence of a daughter sac. The 3-year risk of rupture ranged from <1% to >15% depending on the individual characteristics of patients and aneurysms. External validation indicated good discrimination and calibration properties. InterpretationA simple scoring system that only needs easily available patient and aneurysmal information was constructed. This can be used in clinical decision making regarding management of unruptured cerebral aneurysms. Ann Neurol 2015;77:1050-1059
  • Chengbo Tan, Hideo Shichinohe, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Masaaki Hokari, Shuji Hamauchi, Kiyohiro Houkin
    NEUROPATHOLOGY 35 3 197 - 208 2015年06月 [査読有り][通常論文]
     
    Recently, both basic and clinical studies demonstrated that bone marrow stromal cell (BMSC) transplantation therapy can promote functional recovery of patients with CNS disorders. A non-invasive method for cell tracking using MRI and superparamagnetic iron oxide (SPIO)-based labeling agents has been applied to elucidate the behavior of transplanted cells. However, the long-term safety of SPIO-labeled BMSCs still remains unclear. The aim of this study was to investigate the short-, middle- and long-term safety of the SPIO-labeled allogeneic BMSC transplantation. For this purpose, BMSCs were isolated from transgenic rats expressing green fluorescent protein (GFP) and were labeled with SPIO. The Na/K ATPase pump inhibitor ouabain or vehicle was stereotactically injected into the right striatum of wild-type rats to induce a lacunar lesion (n=22). Seven days after the insult, either BMSCs or SPIO solution were stereotactically injected into the left striatum. A 7.0-Tesla MRI was performed to serially monitor the behavior of BMSCs in the host brain. The animals were sacrificed after 7 days (n=7), 6 weeks (n=6) or 10 months (n=9) after the transplantation. MRI demonstrated that BMSCs migrated to the damage area through the corpus callosum. Histological analysis showed that activated microglia were present around the bolus of donor cells 7 days after the allogeneic cell transplantation, although an immunosuppressive drug was administered. The SPIO-labeled BMSCs resided and started to proliferate around the route of the cell transplantation. Within 6 weeks, large numbers of SPIO-labeled BMSCs reached the lacunar infarction area from the transplantation region through the corpus callosum. Some SPIO nanoparticles were phagocytized by microglia. After 10 months, the number of SPIO-positive cells was lower compared with the 7-day and 6-week groups. There was no tumorigenesis or severe injury observed in any of the animals. These findings suggest that BMSCs are safe after cell transplantation for the treatment of stroke.
  • 山本 雅史, 関 俊隆, 笹森 徹, 飛騨 一利, 奥原 浩之, 村上 望, 田中 小枝子, 川口 朋香, 中根 進児, 渋谷 斉, 重松 明男, 清水 力, 寶金 清博
    脊髄外科 29 1 42 - 46 (一社)日本脊髄外科学会 2015年04月 
    当科で脊髄動静脈瘻(spinal AVF)手術を行った46例を対象に、術中MEPモニタリングの有用性について検討した。その結果、ほぼ全例の被検筋において術中MEPが高振幅で導出可能であった。脊髄硬膜動静脈瘻(dAVF)14例では、全例が上下肢ともに術中MEPの振幅低下を認めず、さらに術後麻痺も認めなかった。dAVFにおける偽陽性率、偽陰性率はともに0%であった。一方、脊髄辺縁部動静脈瘻(perimedullary AVF)では32例中6例が振幅の低下を認め、うち3例では術後麻痺を認めた。perimedullary AVFにおける偽陽性率は9.7%、偽陰性率は0%であった。以上、spinal AVFにおける術中モニタリングは術後麻痺の危険性を予測することが可能であり、有用性が示唆された。
  • Hideo Shichinohe, Chengbo Tan, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Masaaki Hokari, Kiyohiro Houkin, Satoshi Kuroda
    BRAIN RESEARCH 1602 53 - 61 2015年03月 [査読有り][通常論文]
     
    The phosphodiesterase (PDE) 3 inhibitor cilostazol, used as an anti-platelet drug, reportedly can also ameliorate ischemic brain injury. Here, we investigated the effects of cilostazol in a permanent focal ischemia mice model. Male Balb/c mice were subjected to permanent middle cerebral artery occlusion. Mice were then treated with either cilostazol (10 or 20 mg/kg) or vehicle administered at 30 min and 24 h post-ischemia, and infarct volumes were assessed at 48 h post-ischemia. Mice treated with 20 mg/kg of cilostazol or vehicle were sacrificed at 6 h or 24 h post-ischemia and immunohistochemistry was used for brain sections. Treatment with 20 mg/kg of cilostazol significantly reduced infarct volumes to 70.1% of those with vehicle treatment. Immunohistochemistry results for 8-hydroxydeoxyguanosine (OHdG) expression showed that some neurons underwent oxidative stress around the ischemic boundary zone at 6 h post-ischemia. Cilostazol treatment significantly reduced the percentage of 8-0HdGpositive neurons (65.8+33.5% with vehicle and 21.3+9.9% with cilostazol). Moreover, NADPH oxidase (NOX) 2-positive neurons were significantly reduced with cilostazol treatment. In contrast, immunohistochemistry results for phosphorylated cyclic-AMP response element binding protein (pCREB) showed that there were significantly more pCREB-positive neurons around the ischemic boundary zone of cilostazol-treated mice than in those of vehicle-treated mice at 24 h post-ischemia. These results suggested that cilostazol might have multiple mechanisms of action to ameliorate ischemic tissue damage, by attenuating oxidative stress mediated by suppressing NOX2 expression by ischemic neurons and an anti-apoptotic effect mediated through the pCREB pathway. (C) 2015 Elsevier B.V. All rights reserved.
  • Tomohiro Yamauchi, Yasumasa Kuroda, Takahiro Morita, Hideo Shichinohe, Kiyohiro Houkin, Mari Dezawa, Satoshi Kuroda
    PLOS ONE 10 3 e0116009  2015年03月 [査読有り][通常論文]
     
    ObjectiveBone marrow stromal cells (BMSCs) are heterogeneous and their therapeutic effect is pleiotropic. Multilineage-differentiating stress enduring (Muse) cells are recently identified to comprise several percentages of BMSCs, being able to differentiate into triploblastic lineages including neuronal cells and act as tissue repair cells. This study was aimed to clarify how Muse and non-Muse cells in BMSCs contribute to functional recovery after ischemic stroke.MethodsHuman BMSCs were separated into stage specific embryonic antigen-3-positive Muse cells and -negative non-Muse cells. Immunodeficient mice were subjected to permanent middle cerebral artery occlusion and received transplantation of vehicle, Muse, non-Muse or BMSCs (2.5x10(4) cells) into the ipsilateral striatum 7 days later.ResultsMotor function recovery in BMSC and non-Muse groups became apparent at 21 days after transplantation, but reached the plateau thereafter. In Muse group, functional recovery was not observed for up to 28 days post-transplantation, but became apparent at 35 days post-transplantation. On immunohistochemistry, only Muse cells were integrated into peri-infarct cortex and differentiate into Tuj-1- and NeuN-expressing cells, while negligible number of BMSCs and non-Muse cells remained in the peri-infarct area at 42 days post-transplantation.ConclusionsThese findings strongly suggest that Muse cells and non-Muse cells may contribute differently to tissue regeneration and functional recovery. Muse cells may be more responsible for replacement of the lost neurons through their integration into the peri-infarct cortex and spontaneous differentiation into neuronal marker-positive cells. Non-Muse cells do not remain in the host brain and may exhibit trophic effects rather than cell replacement.
  • Yasushi Takagi, Susumu Miyamoto, COSMO-Japan Study Group, Kiyohiro Houkin, Joji Nakagawara, Kuniaki Ogasawara, Teiji Tominaga, Yoshikazu Okada, Tadashi Nariai, Satoshi Kuroda, Yukihiko Fujii, Toshihiko Wakabayashi, Kazuo Yamada, Susumu Miyamoto, Hiroyuki Nakase, Koji Iihara, Toshio Matsushima, Izumi Nagata, Toshiya Murai, Tomohisa Okada, Yasushi Takagi
    Neurologia Medico-Chirurgica 55 3 199 - 203 2015年02月20日 [査読有り][通常論文]
     
    Moyamoya disease is a cerebrovascular occlusive disease characterized by progressive stenosis or by occlusion at the terminal portion of the bilateral internal carotid arteries. The unusual vascular network (moyamoya vessels) at the base of the brain with this disease as collateral channels is developed in this disease. Social independence because of cognitive impairment has recently been recognized as an important unsolved social issue with adult moyamoya disease. The patients with cognitive impairment have difficulty in proving their status because the standard neuroradiological and neuropsychological methods to define cognitive impairment with moyamoya disease are not determined. These patients with cognitive impairment should be supported by social welfare as psychologically handicapped persons. Thus Cognitive Dysfunction Survey of the Japanese Patients with Moyamoya Disease (COSMO-JAPAN study) is planned. In this study, we want to establish a standard finding of the cognitive impairment in patients with moyamoya disease.
  • 骨転移をきたした小脳膠芽腫の1例
    湊川 英樹, 森 崇, 鬼丸 力也, 小野寺 俊輔, 白土 博樹, 東海林 菊太郎, 吉田 道春, 小林 浩之, 寳金 清博, 菅野 宏美, 田中 伸哉
    Japanese Journal of Radiology 33 Suppl. 4 - 4 (公社)日本医学放射線学会 2015年02月
  • Uchino Haruto, Ito Masaki, Kazumata Ken, Nakayama Naoki, Kuroda Satoshi, Houkin Kiyohiro
    STROKE 46 2015年02月 [査読有り][通常論文]
  • Shichinohe Hideo, Kuroda Satoshi, Tan Chengbo, Abumiya Takeo, Nakayama Naoki, Kazumata Ken, Hokari Masaaki, Houkin Kiyohiro
    STROKE 46 2015年02月 [査読有り][通常論文]
  • Shimoda Yusuke, Nakayama Naoki, Hokari Masaaki, Abumiya Takeo, Shichinohe Hideo, Kazumata Ken, Houkin Kiyohiro
    STROKE 46 2015年02月 [査読有り][通常論文]
  • Kurisu Kota, Abumiya Takeo, Nakamura Hideki, Shimbo Daisuke, Kazumata Ken, Nakayama Naoki, Shichinohe Hideo, Hokari Masaaki, Osanai Toshiya, Shimizu Hiroshi, Houkin Kiyohiro
    STROKE 46 2015年02月 [査読有り][通常論文]
  • Abumiya Takeo, Fujima Noriyuki, Kudo Kohsuke, Ishi Yukitomo, Gekka Masayuki, Shichinohe Hideo, Kazumata Ken, Nakayama Naoki, Houkin Kiyohiro
    STROKE 46 2015年02月 [査読有り][通常論文]
  • Shigeru Yamaguchi, Kenji Hirata, Sadahiro Kaneko, Hiroyuki Kobayashi, Tohru Shiga, Kentaro Kobayashi, Rikiya Onimaru, Hiroki Shirato, Nagara Tamaki, Shunsuke Terasaka, Kiyohiro Houkin
    ACTA NEUROCHIRURGICA 157 2 187 - 194 2015年02月 [査読有り][通常論文]
     
    Although histological diagnosis is indispensable in treating primary central nervous system lymphoma (PCNSL), we sometimes face an intractable situation in which tissue can be obtained only from a deep-seated brain lesion. In place of a histological diagnosis, the diagnostic adequacy of the combined use of 18 F-FDG PET and corticosteroid administration for PCNSL located in a deep-seated brain structure is reported.Patients with a deep-seated tumor were treated as having PCNSL without histological confirmation, based on the following criteria: (1) there was no evidence of systemic malignancy; (2) the tumor showed an extremely high FDG uptake relative to normal gray matter on pretreatment 18 F-FDG PET; (3) the tumor decreased in size 1 week after diagnostic therapy by corticosteroid administration on contrast-enhanced T1-weighted magnetic resonance imaging (MRI). FDG uptake of the lesion was evaluated by the maximum of standardized uptake values (SUVmax) and tumor-to-normal ratio of the SUV (T/N ratio). The extent of the tumor reduction was calculated by volumetric analysis for the treatment response to corticosteroid administration.Ten patients (4 males and 6 females) matched these criteria. On pretreatment 18 F-FDG PET, mean SUVmax in the tumor was 24.8 (8.75-60.75), and mean T/N ratio was 3.24 (2.17-5.12). The extent of tumor volume reduction was shown to be 21 to 68 % 1 week after diagnostic therapy by corticosteroids. Mean total dose and duration of corticosteroids were 719 mg as prednisolone and 6.5 days, respectively. Nine patients achieved complete response and one patient achieved partial response on MRI after standard treatment for PCNSL with high-dose methotrexate and/or whole-brain irradiation.Although the value of biopsy is universal, combining 18 F-FDG PET and corticosteroid administration is an important alternative method that may lead to the diagnosis of deep-seated PCNSLs in cases with intractable histopathological confirmations.
  • Ken Kazumata, Khin Khin Tha, Hisashi Narita, Ichiro Kusumi, Hideo Shichinohe, Masaki Ito, Naoki Nakayama, Kiyohiro Houkin
    STROKE 46 2 354 - 360 2015年02月 [査読有り][通常論文]
     
    Background and Purpose-The mechanisms underlying frontal lobe dysfunction in moyamoya disease (MMD) are unknown. We aimed to determine whether chronic ischemia induces subtle microstructural brain changes in adult MMD and evaluated the association of changes with neuropsychological performance. Methods-MRI, including 3-dimensional T1-weighted imaging and diffusion tensor imaging, was performed in 23 adult patients with MMD and 23 age-matched controls and gray matter density and major diffusion tensor imaging indices were compared between them; any alterations in the patients were tested for associations with age, ischemic symptoms, hemodynamic compromise, and neuropsychological performance. Results-Decrease in gray matter density, associated with hemodynamic compromise (P<0.05), was observed in the posterior cingulate cortex of patients with MMD. Widespread reduction in fractional anisotropy and increases in radial diffusivity and mean diffusivity in some areas were also observed in bilateral cerebral white matter. The fractional anisotropy (r=0.54; P<0.0001) and radial diffusivity (r=-0.41; P<0.01) of white matter significantly associated with gray matter density of the cingulate cortex. The mean fractional anisotropy of the white matter tracts of the lateral prefrontal, cingulate, and inferior parietal regions were significantly associated with processing speed, executive function/attention, and working memory. Conclusions-In adult MMD, there were more white matter abnormalities than gray matter changes. Disruption of white matter may play a pivotal role in the development of cognitive dysfunction.
  • Tatsushi Mutoh, Ken Kazumata, Yuka Yokoyama, Tatsuya Ishikawa, Yasuyuki Taki, Shunsuke Terasaka, Kiyohiro Houkin
    Journal of Neurosurgical Anesthesiology 27 1 7 - 15 2015年01月12日 [査読有り][通常論文]
     
    Background: Recent clinical data suggest that postoperative hemodynamic profile and fluid management may differ in aneurysmal subarachnoid hemorrhage patients depending on the treatment option: surgical clipping or endovascular coiling. Our aim was to determine the differences in hemodynamic parameters between the 2 modalities using an advanced transpulmonary thermodilution technique. Methods: We studied 73 consecutive aneurysmal subarachnoid hemorrhage patients treated with either clipping or coiling. Transpulmonary thermodilution was established for monitoring cardiac index, global end-diastolic volume index (GEDI), and extravascular lung water index (ELWI). Blood biochemical markers were sampled in parallel. Results: Hypovolemia (GEDI: 656±72 vs. 713±40 mL/m2 P=0.0001) and elevated plasma brain natriuretic peptide (114.0±56.7 vs. 68.6±47.4 pg/mL P=0.0004) were evident on initial measurements in the clipping group. The number of fluid challenges until normalization of GEDI and ROC-based prediction of the responders (GEDI ≥10%) during vasospasm risk period (day 4 to 14 of ictus) were less with coiling than with clipping (P< 0.05). Therapy-related pulmonary edema was detected only in the clipping group (8%, n=3). Although length of intensive care unit stay was shorter in the coiling group (P=0.016), incidences of delayed ischemia (13% vs. 11% P=0.50) and poor functional outcome on modified Rankin Scale score 4 to 6 at 1 month (37% vs. 46% P=0.30) were not statistically different. Conclusions: Surgical clipping is associated with higher cardiac output and hypovolemia in the early postoperative stage and poorer preload responsiveness to volume therapy during the vasospasm risk period compared with endovascular coiling.
  • Hideo Shichinohe, Takeshi Ishihara, Koji Takahashi, Yoshikazu Tanaka, Michiyuki Miyamoto, Tomohiro Yamauchi, Hisayasu Saito, Hiroshi Takemoto, Kiyohiro Houkin, Satoshi Kuroda
    NEUROREHABILITATION AND NEURAL REPAIR 29 1 80 - 89 2015年01月 [査読有り][通常論文]
     
    Background. Transplantation of bone marrow stromal cells (BMSCs) may contribute to functional recovery after stroke. This study was designed to clarify their mechanisms, trophic effects of neurotrophic factors, and neural differentiation. Methods. Mouse neurons exposed to glutamate were cocultured with mouse BMSCs. Either neutralizing antibodies against brain-derived neurotrophic factor (BDNF) or nerve growth factor (NGF) or Trk inhibitor K252a was added to explore the mechanism of their protective effects. Fluorescence in situ hybridization (FISH) was used to assess BDNF or NGF mRNA expression in BMSCs. The mice were subjected to permanent focal ischemia, and 7 days later, either BMSCs or the vehicle was stereotactically transplanted into the ipsilateral striatum. The mouse brains were processed for FISH and immunostaining 2 or 4 weeks after transplantation. Results. BMSCs significantly ameliorated glutamate-induced neuronal death. Treatment with anti-BDNF antibody significantly reduced their protective effects. FISH analysis showed that the majority of BMSCs expressed BDNF and NGF mRNA in vitro. BMSC transplantation significantly improved the survival of neurons in peri-infarct areas. FISH analysis revealed that approximately half of BMSCs expressed BDNF and NGF mRNA 2 weeks after transplantation; however, the percentage of BDNF and NGF mRNA-positive cells decreased thereafter. Instead, the percentage of microtubule-associated protein 2-positive BMSCs gradually increased during 4 weeks after transplantation. Conclusions. These findings strongly suggest that BDNF may be a key factor underlying the trophic effects of BMSCs. BMSCs might exhibit the trophic effect in the early stage of cell therapy and the phenotypic change toward neural cells thereafter.
  • 遠藤将吾, 寺坂俊介, 小林浩之, 山口秀, 茂木洋晃, 金子貞洋, 井口晶裕, 長祐子, 大島淳二郎, 寺坂友佳代, 杉山未奈子, 橋本孝之, 鬼丸力也, 清水伸一, 有賀正, 白戸博樹, 寳金清博
    日本小児血液・がん学会雑誌(Web) 52 3 2015年
  • Toshitaka Seki, Kazutoshi Hida, Syunsuke Yano, Takeshi Aoyama, Izumi Koyanagi, Kiyohiro Houkin
    Asian Spine Journal 9 6 935 - 941 2015年 [査読有り][通常論文]
     
    Study Design: A retrospective study. Purpose: The purpose of this study was to obtain useful information for establishing the guidelines for treating high-grade spinal cord gliomas. Overview of Literature: The optimal management of high-grade spinal cord gliomas remains controversial. We report the outcomes of the surgical management of 14 high-grade spinal glioma. Methods: We analyzed the outcomes of 14 patients with high-grade spinal cord gliomas who were surgically treated between 1989 and 2012. Survival was charted with the Kaplan-Meier plots and comparisons were made with the log-rank test. Results: None of the patients with high-grade spinal cord gliomas underwent total resection. Subtotal resection was performed in two patients, partial resection was performed in nine patients, and open biopsy was performed in three patients. All patients underwent postoperative radiotherapy and six patients further underwent radiation cordotomy. The median survival time for patients with high-grade spinal cord gliomas was 15 months, with a 5-year survival rate of 22.2%. The median survival time for patients with World Health Organization grade III tumors was 25.5 months, whereas the median survival time for patients with glioblastoma multiforme was 12.5 months. Both univariate and multivariate Cox proportional hazards models demonstrated a significant effect only in the group that did not include cervical cord lesion as a factor associated with survival (p =0.04 and 0.03). Conclusions: The surgical outcome of patients diagnosed with high-grade spinal cord gliomas remains poor. Notably, only the model which excluded cervical cord lesions as a factor significantly predicted survival.
  • Junya Aoki, Kensaku Shibazaki, Masaki Ito, Naoki Saji, Junichi Uemura, Kiyohiro Houkin, Kazumi Kimura
    INTERNAL MEDICINE 54 8 971 - 974 2015年 [査読有り][通常論文]
     
    We herein report a case of ischemic stroke in a 69-year-old man with unilateral moyamoya vessels originating from the proximal portion of the left middle cerebral artery. In addition, digital-subtraction angiography demonstrated a string-of-beads-like appearance in the cavernous portion of the left internal carotid artery. A genetic analysis revealed a heterozygous c.14576G>A variant in ring finger protein 213. The patient's younger brother had a history of hemorrhagic stroke and had been diagnosed with moyamoya disease. We finally considered that the unilateral moyamoya vessel and string-of-beads appearance observed in the current case were not simply caused by atherosclerosis, but rather represented symptoms within the moyamoya spectrum.
  • Horita Y, Mikami T, Houkin K, Mikuni N
    Surgical neurology international 6 Suppl 9 S291 - 4 2015年 [査読有り][通常論文]
  • Takeshi Mikami, Satoko Ochi, Kiyohiro Houkin, Yukinori Akiyama, Masahiko Wanibuchi, Nobuhiro Mikuni
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 24 1 17 - 23 2015年01月 [査読有り][通常論文]
     
    Background: Epilepsy cannot always be recognized in patients with moyamoya disease. In this report, the clinical features of patients with epilepsy were evaluated for assessing the predictive factors of epilepsy in moyamoya disease. Methods: A total of 64 consecutive patients with moyamoya disease were included in this study. During their follow-up periods, 7 patients were diagnosed with epilepsy. Then, the patients with epilepsy were compared with the patients without epilepsy regarding their clinical features. Results: Analysis of patient background characteristics revealed a significantly higher incidence of epilepsy in patients with high modified Rankin Scale (mRS) scores, high cerebrovascular attack scores, onset age of 3 years or less, early seizures, cortical involvement, stroke subtype, and diffuse brain atrophy. A logistic analysis of epilepsy data revealed significant differences between the 2 groups in mRS score, cerebrovascular attack score, onset age 3 years or less, early seizure, cortical involvement, stroke subtype, and diffuse brain atrophy. Of these, significant differences were noted in 3 items (mRS score, early seizure, and diffuse brain atrophy) on multivariate analysis. These 3 items were selected as the basis of our new moyamoya disease epilepsy risk scale (MDERS), which we then evaluated. The cutoff value estimated by the receiver operating characteristic curve was set at 1 (sensitivity,.857; specificity,.825) or 2 (sensitivity,.571; specificity, 1.000). Conclusions: Epilepsy in moyamoya disease is associated with clinical factors and is not an independent category. For prediction of epilepsy in moyamoya disease, MDERS is a simple and convenient assessment scale.
  • Tomohiro Yamauchi, Hisayasu Saito, Masaki Ito, Hideo Shichinohe, Kiyohiro Houkin, Satoshi Kuroda
    TRANSLATIONAL STROKE RESEARCH 5 6 701 - 710 2014年12月 [査読有り][通常論文]
     
    Autologous human bone marrow stromal cells (hBMSCs) should be expanded in the animal serum-free condition within clinically relevant periods in order to secure safe and effective cell therapy for ischemic stroke. This study was aimed to assess whether the hBMSCs enhance their proliferation capacity and provide beneficial effect in the infarct brain when cultured with platelet lysate (PL) and granulocyte-colony stimulating factor (G-CSF). The hBMSCs were cultured in the fetal calf serum (FCS)-, PL-, or PL/G-CSF-containing medium. Cell growth kinetics was analyzed. The hBMSCs-PL, hBMSC-PL/G-CSF, or vehicle was stereotactically transplanted into the ipsilateral striatum of the rats subjected to permanent middle cerebral artery occlusion 7 days after the insult. Motor function was assessed for 8 weeks, and the fate of transplanted hBMSCs was examined using immunohistochemistry. As the results, the hBMSCs-PL/G-CSF showed more enhanced proliferation than the hBMSCs-FCS and hBMSCs-PL. Transplantation of hBMSCs expanded with the PL- or PL/G-CSF-containing medium equally promoted functional recovery compared with the vehicle group. Histological analysis revealed that there were no significant differences in their migration, survival, and neural differentiation in the infarct brain between the hBMSCs-PL and hBMSCs-PL/G-CSF. These findings strongly suggest that the combination of PL and G-CSF may accelerate hBMSC expansion and serve safe cell therapy for patients with ischemic stroke at clinically relevant timing.
  • Satoshi Kobayashi, Michiyuki Miyamoto, Shinichiro Shinada, Yukitomo Ishi, Yusuke Shimoda, Kazuyoshi Yamazaki, Satoshi Ushikoshi, Tomonori Ooka, Yoshiro Matsui, Kiyohiro Houkin
    Neurological Surgery 42 11 1057 - 1062 2014年11月01日 [査読有り][通常論文]
     
    The number of patients with a ventricular assist device (VAD) will increase with the spread of heart transplantation in Japan. On the other hand, it is likely that VADs could cause cerebral embolism. However, there are few reports about endovascular therapy for intracranial embolic infarction from VAD The authors report successful acute endovascular therapy for cerebral embolism A 19-year-old woman with a VAD who received anti-coagulant treatment by warfarin sodium presented disturbance of consciousness and right hemiparesis CT scan showed early CT sign in the left middle cerebral artery (MCA) area. 3D-CTA demonstrated occlusion of the left MCA and basilar artery (BA). We first performed endovascular recanalization in the left MCA. because IV tPA was ineligible. The left MCA was recanalized with TICI 2b perfusion and her symptoms were significantly improved. The treatment of the VAD patient reveals important issues First, the femoral puncture requires ultrasound due to pulseless femoral artery. Second, the access route is an intact artery because of the anatomy of the VAD. Third, even if the patient has a hemorrhagic complication by intervention, the patient must be kept on anti-coagulant treatment because the VAD requires it Careful consideration should be given to recanalization of occlusive vessels.
  • Masayuki Gekka, Toshitaka Seki, Kazutoshi Hida, Toshiya Osanai, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 54 11 936 - 940 2014年11月 [査読有り][通常論文]
     
    Perimedullary arteriovenous fistula (AVF) shunts occur on the spinal cord surface and can be treated surgically or by endovascular embolization. In contrast, the nidus of an intramedullary arteriovenous malformation (AVM) is located in the spinal cord and is difficult to treat surgically or by endovascular techniques. The benefits of radiotherapy for treating intramedullary AVM have been published, but are anecdotal and consist largely of case reports. We present a case of combined cervical intramedullary AVM and perimedullary AVF which received surgical treatment within a hybrid operating room (OR) after 5 years of focus fractionated radiotherapy. A 37-year-old male presented with stepwise worsening myelopathy. Magnetic resonance imaging and spinal angiography revealed intramedullary AVM and perimedullary AVF at the C3 to C5 levels. In order to reduce nidus size and blood flow, we first performed focal fractionated radiotherapy. Five years later, the lesion volume was reduced. Following this, direct surgery was performed by an anterior approach using corpectomy in the hybrid OR. The spinal cord was monitored by motor-evoked potential throughout the surgery. Complete obliteration of the fistulous connection was confirmed by intraoperative indocyanine green video-angiography and intraoperative angiography, preserving the anterior spinal artery. We conclude that surgical treatment following focal fractionated radiotherapy may become one strategy for patients who are initially deemed ineligible for endovascular embolization and surgical treatment. Furthermore, the hybrid OR enables safe and precise treatment for spinal vascular disorders in the fields of endovascular treatment and neurosurgery.
  • Miyamoto M, Nakayama N, Hokari M, Kuroda S, Takikawa S, Houkin K
    NMC case report journal 1 1 9 - 11 2014年10月 [査読有り][通常論文]
     
    Because of the wide spread of magnetic resonance image (MRI), it may be increasing to find a dissecting aneurysm (DA) of the posterior inferior cerebellar artery (PICA) that causes headache without associated hemorrhage or infarction. Generally, surgical treatment might be considered in cases of DA with hemorrhage. However, the treatment of an unruptured DA with headache or infarction has not been well established. This is the first report regarding the pathology of an intact DA of the PICA that presents with headache only, and the pathological findings showed interesting figures. A 44-year-old man with an unruptured DA of the left PICA presented with sudden left occipital headache. MRI revealed no hemorrhage or infarction. Magnetic resonance angiography (MRA) showed growth of the DA 12 days after the onset of headache. Therefore, surgery was performed to prevent aneurysmal rupture. This DA aneurysm was trapped and removed after an occipital artery (OA)-PICA anastomosis was performed. The surgery was performed without complication. Pathological findings showed folding of the internal elastic lamina, and the true lumen was torn by the false lumen. The dissection reached the adventitia and the wall had numerous macrophages. Pathological findings might help understanding the etiology of DAs and inflammation might play an important role in DAs.
  • Haruto Uchino, Ken Kazumata, Masaki Ito, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
    ACTA NEUROCHIRURGICA 156 9 1753 - 1760 2014年09月 [査読有り][通常論文]
     
    Postoperative hyperperfusion is a potential complication of the direct bypass procedure for moyamoya disease (MMD). However, no reliable modality is available yet for the prediction of postoperative hyperperfusion during surgery for MMD. This study aimed to investigate whether semiquantitative analysis of indocyanine green (ICG) videoangiography could contribute to the prediction of postoperative hyperperfusion in MMD. This study included 12 hemispheres from 10 patients who underwent surgical revascularization for MMD. Intraoperative ICG videoangiography was performed before and after a direct bypass procedure. The ICG intensity-time curves were semiquantitatively analyzed to evaluate cortical perfusion by calculating the blood flow index (BFI). Using single-photon emission computed tomography (SPECT), postoperative cerebral blood flow measurements were performed thrice: immediately, and 2 and 7 days after surgery. BFI significantly increased from 21.3 +/- 10.5 to 38.4 +/- 20.0 after bypass procedures in all the hemispheres (p < 0.01). The ratio of BFI before and after the bypass procedure was 2.4 +/- 2.0, ranging from 0.5 to 8.0. Postoperative hyperperfusion was observed in nine of the 12 operated hemispheres within 7 days after surgery. Of these, three hemispheres developed hyperperfusion immediately after surgery. In the adult cases, the increase in the ratio of BFI after bypass was significantly greater in those who developed hyperperfusion immediately after surgery than in those who did not (6.5 +/- 0.5 vs. 1.8 +/- 2.1, p < 0.01). In contrast, no significant increase in BFI was observed in the pediatric MMD patients who experienced immediate hyperperfusion. No correlation between the changes in BFI and the occurrence of delayed hyperperfusion was observed. Our results suggest that semiquantitative analysis of BFI by intraoperative ICG videoangiography is useful in evaluating changes in cortical perfusion after bypass procedures for MMD and can predict the occurrence of early-onset hyperperfusion in MMD patients after direct bypass.
  • Satoshi Kuroda, Daina Kashiwazaki, Kenji Hirata, Tohru Shiga, Kiyohiro Houkin, Nagara Tamaki
    STROKE 45 9 2717 - 2721 2014年09月 [査読有り][通常論文]
     
    Background and Purpose-This prospective study was aimed to evaluate the effects of surgical revascularization on cerebral oxygen metabolism in moyamoya disease. Methods-This study included totally 69 hemispheres of 42 patients who underwent superficial temporal artery to middle cerebral artery anastomosis and indirect bypass for moyamoya disease between 2000 and 2011. There were 12 children and 30 adults. MRI and O-15-gas positron emission tomography were performed before and 3 to 4 months after surgery. Hemodynamic and metabolic parameters were precisely quantified and statistically analyzed. Results-Preoperative positron emission tomographic scans revealed that cerebral blood flow was decreased, cerebral blood volume was increased, and cerebral metabolic rate for oxygen was decreased in both pediatric and adult patients. Cerebral metabolic rate for oxygen reduction was observed in approximate to 80% of pediatric (16/21; 76%) and adult hemispheres (38/48; 79%). Surgical revascularization resolved hemodynamic compromise in all operated hemispheres. Cerebral metabolic rate for oxygen significantly improved in pediatric patients without parenchymal lesions (n=8), but not those with parenchymal lesions (n=8). Multivariate analysis revealed that cerebral metabolic rate for oxygen significantly improved in younger adult patients without parenchymal lesions (P=0.0264; odds ratio, 0.88; 95% confidence interval, 0.79-0.99). Conclusions-Cerebral oxygen metabolism is significantly depressed in approximate to 80% of the involved hemispheres of moyamoya disease and improves in pediatric and younger adult patients without parenchymal lesions after bypass surgery. Cerebral oxygen metabolism may be reversibly depressed in response to cerebral ischemia in them although the underlying mechanisms are still unclear.
  • Ken Kazumata, Masaki Ito, Kikutaro Tokairin, Yasuhiro Ito, Kiyohiro Houkin, Naoki Nakayama, Satoshi Kuroda, Tatsuya Ishikawa, Hiroyasu Kamiyama
    JOURNAL OF NEUROSURGERY 121 2 432 - 440 2014年08月 [査読有り][通常論文]
     
    Object. Although combined direct and indirect anastomosis in patients with moyamoya disease immediately increases cerebral blood flow, the surgical procedure is more complex. Data pertinent to the postoperative complications associated with combined bypass are relatively scarce compared with those associated with indirect bypass. This study investigated the incidence and characteristics of postoperative stroke in combined bypass and compared them with those determined from a literature review to obtain data from a large population. Methods. A total of 358 revascularization procedures in 236 patients were retrospectively assessed by reviewing clinical charts and radiological data. PubMed was searched for published studies on surgical treatment to determine the incidence of postoperative complications in a larger population. Results. Seventeen instances of postoperative stroke were observed in 16 patients (4.7% per surgery, 95% CI 2.8%-7.5%). Postoperative stroke was more frequent (7.9% per surgery) in adults than in pediatric patients (1.7% per surgery, OR 4.07, 95% CI 1.12-14.7; p < 0.05). Acute progression of stenoocclusive changes were identified in the major cerebral arteries (anterior cerebral artery, n = 3; middle cerebral artery, n = 1; posterior cerebral artery, n = 2). The postoperative stroke rate was comparable with that (5.4%) determined from a literature search that included studies reporting more than 2000 direct/combined procedures. No differences in the stroke rates between the direct/combined and indirect procedures were found. In the literature review, direct/combined bypass was more often associated with excellent revascularization (angiographic pacification greater than two-thirds) than indirect bypass (p < 0.05). Conclusions. This experience of 358 consecutive procedures is one of the largest series for which the postoperative stoke rate for direct/combined bypass performed with a unified strategy has been reported. A systematic review confirmed that the postoperative stroke rate for the direct/combined procedure was comparable to that for the indirect procedure.
  • Hiroaki Motegi, Yuuta Kamoshima, Shunsuke Terasaka, Hiroyuki Kobayashi, Kiyohiro Houkin
    NEUROPATHOLOGY 34 4 378 - 385 2014年08月 [査読有り][通常論文]
     
    Cancer stem cells are thought to be closely related to tumor progression and recurrence, making them attractive therapeutic targets. Stem cells of various tissues exist within niches maintaining their stemness. Glioblastoma stem cells (GSCs) are located at tumor capillaries and the perivascular niche, which are considered to have an important role in maintaining GSCs. There were some extracellular matrices (ECM) on the perivascular connective tissue, including type 1 collagen. We here evaluated whether type 1 collagen has a potential niche for GSCs. Imunohistochemical staining of type 1 collagen and CD133, one of the GSCs markers, on glioblastoma (GBM) tissues showed CD133-positive cells were located in immediate proximity to type 1 collagen around tumor vessels. We cultured human GBM cell lines, U87MG and GBM cells obtained from fresh surgical tissues, T472 and T555, with serum-containing medium (SCM) or serum-free medium with some growth factors (SFM) and in non-coated (Non-coat) or type 1 collagen-coated plates (Col). The RNA expression levels of CD133 and Nestin as stem cell markers in each condition were examined. The Col condition not only with SFM but SCM made GBM cells more enhanced in RNA expression of CD133, compared to Non-coat/SCM. Semi-quantitative measurement of CD133-positive cells by immunocytochemistry showed a statistically significant increase of CD133-positive cells in Col/SFM. In addition, T472 cell line cultured in the Col/SFM had capabilities of sphere formation and tumorigenesis. Type 1 collagen was found in the perivascular area and showed a possibility to maintain GSCs. These findings suggest that type 1 collagen could be one important niche component for CD133-positive GSCs and maintain GSCs in adherent culture.
  • Shigeru Yamaguchi, Kenji Hirata, Hiroyuki Kobayashi, Tohru Shiga, Osamu Manabe, Kentaro Kobayashi, Hiroaki Motegi, Shunsuke Terasaka, Kiyohiro Houkin
    ANNALS OF NUCLEAR MEDICINE 28 7 603 - 609 2014年08月 [査読有り][通常論文]
     
    F-18-FDG PET has become one of the most important methods for studying malignant lymphoma, but its diagnostic role for primary central nervous system lymphoma (PCNSL) has not been established. The aim of this study was to determine the appropriate cut-off values of FDG uptake and to investigate how corticosteroid administration influences PCNSL.We retrospectively reviewed 82 patients with contrast-enhanced brain tumors who underwent an FDG PET scan at onset, including 19 PCNSLs. FDG uptake of the lesion was assessed by the maximum standardized uptake value (SUVmax) and the ratio of tumor to normal contralateral cortex activity (T/N ratio). Receiver operating characteristic (ROC) curves were generated from the SUVmax and T/N ratios. To investigate the influence of corticosteroid application before a FDG PET scan, we evaluated the association between the FDG uptake of the lesion and the cumulative dose of corticosteroid administration on 13 PCNSL patients who had received steroid treatment before an FDG PET examination.The mean FDG SUVmax and T/N ratio of PCNSLs were 22.6 and 2.79, respectively, and these values were significantly higher than those of the other malignant brain tumors. ROC analysis indicated that the evaluation of FDG uptake using the T/N ratio was more reliable than the SUVmax with respect to the differential diagnosis. When PCNSL patients went without steroid application before FDG PET, the accuracy of the T/N ratio with a cut-off point of 2.0 was 91.1 %, the sensitivity was 94.7 %, and the specificity was 87.3 %. Although there are no significant differences in the FDG T/N ratio for PCNSL patients with or without steroid treatment, a negative correlation was found between the T/N ratio and cumulative dose of corticosteroid before PET study (r = -0.71, p = 0.032).We concluded that the T/N ratio was superior to SUVmax for FDG uptake assessment as for distinguishing PCNSLs from other malignant brain tumors; the appropriate T/N ratio cut-off point was 2.0. In addition, FDG uptake could be influenced by cumulative doses of corticosteroid before a PET scan, and thus this fact should be taken into consideration when evaluating FDG PET for PCNSL diagnosis.
  • Ken Kazumata, Hiroyasu Kamiyama, Tatsuya Ishikawa, Toshitaka Nakamura, Shunsuke Terasaka, Kiyohiro Houkin
    NEUROSURGICAL REVIEW 37 3 493 - 500 2014年07月 [査読有り][通常論文]
     
    Strategic cervical internal carotid occlusion is employed either temporarily or permanently in various neurosurgical procedures. The aim of the present study was to assess changes in cortical arterial pressure during cervical internal carotid cross-clamping before and after the placement of radial artery (RA) graft bypass in the treatment of complex carotid artery aneurysms. Perfusion pressure of the middle cerebral artery (MCA) was assessed in 22 patients with complex carotid aneurysm treated with RA graft bypass. Regional cerebral blood flow was assessed postoperatively using single-photon computed tomography. Mean cortical blood pressure (mcBP) was found to be 48.2 +/- 24.2 and 97.0 +/- 24.0 % of baseline after clamping the cervical internal carotid artery and opening the RA graft bypass, respectively. Cerebral perfusion pressure estimated by the mcBP failed to sustain a critical limit of greater than 70 mmHg under craniotomy in 16 out of 20 (80 %) patients. There was an inverse correlation in mcBP between the baseline and after the placement of the RA graft bypass (r = 0.66, P < 0.005). Postoperative regional cerebral blood flow in the MCA territory on the ipsilateral side of the aneurysm was 97 +/- 7 % of that of the contralateral side after internal carotid artery (ICA) ligation combined with RA graft bypass. Substantial pressure reductions in cerebral cortical arteries were observed during the cervical internal carotid cross-clamping. Perfusion pressure in peripheral cortical arteries after the placement of the RA graft bypass was comparable to the state before ICA clamping.
  • Hiroaki Motegi, Shunsuke Terasaka, Hideaki Shiraishi, Kiyohiro Houkin
    CHILDS NERVOUS SYSTEM 30 7 1313 - 1315 2014年07月 [査読有り][通常論文]
     
    Artery of Percheron (AOP) is a rare variant of thalamoperforating artery with a single common trunk feeding both thalami and with or without contribution to the rostral midbrain. We report the first case of thalamic arteriovenous malformation (AVM) fed by AOP with hemorrhagic onset. A 12-month-old girl suddenly weakened and developed coma. Left thalamic hemorrhage was detected with the third and both lateral ventricles' hematoma. Thalamic AVM was discovered to be fed by an AOP originating from the contralateral posterior cerebral artery. Endovascular embolization of AVM was impossible due to a risk of bilateral thalamic infarction and anatomical inaccessibility. Thalamic AVM was removed by high superior parietal approach without new neurological symptoms. The first case of thalamic AVM fed by AOP originating from the contralateral posterior cerebral artery is reported. Surgical removal of AVM would be at the heart of treatment in order to avoid bilateral thalamic infarction.
  • Ken Kazumata, Daisuke Shinbo, Masaki Ito, Hideo Shichinohe, Satoshi Kuroda, Naoki Nakayama, Kiyohiro Houkin
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 23 6 1421 - 1428 2014年07月 [査読有り][通常論文]
     
    Background: Adult moyamoya disease (MMD) is known to have high incidence of cerebral microbleeds (cMBs); however, the clinical significance still remains unclear. We investigated the frequency of cMBs in a large number of patients and analyzed the patterns of MB distribution in association with the location of the hematoma and moyamoya vessels. Methods: We studied 259 consecutive patients with MMD using prospectively collected database. One hundred ninety-one patients were eligible for the present study, and image analysis was performed retrospectively. The presence of cMBs and remains of hemorrhage were determined using gradient-echo T2*-weighted sequence (1.5 T). The development of moyamoya vessels was assessed on source images of time-of-flight magnetic resonance angiography. The analysis consists of descriptive assessment of the spatial relationship between cMB, remains of hemorrhage, and moyamoya vessels. Statistical analysis was performed to calculate relative risk ratio in the presence of cMBs in relation to the remains of hemorrhage (macrohematoma), age of onset, and the presence of concomitant moyamoya vessels. Results: Thirty MBs were observed in 20 adult MMD patients (16.9%). MBs were located predominantly in the periventricular white matter (63.3%) followed by the basal ganglia/thalami (20%). Comparing the patients with cMBs from those without, hematoma was more frequently observed in patients with cMBs (odds ratio [OR] 4.29; 95% confidence interval [CI] 1.58-11.62; P = .0062). Patients with adult onset was more likely to demonstrate cMBs (14.4%) compared with the patients with pediatric onset (4.1%) (OR 3.93; 95% CI 1.11-13.91). Moyamoya vessels appeared in the lateral part of the trigon, and the periventricular white matter was significantly associated with the presence of cMBs (lateral part of the trigon; OR 3.29 [1.59-6.82], P - .0019, periventricle of the body of lateral ventricle; OR 2.40 [1.20-4.79], P = .0214, respectively). cMBs accompanied concomitant arteries in 23 (76.7%) lesions. The subependymal-leptomeningeal artery anastomosis was the most common pattern (n = 20, 66.7%). Conclusions: Spatial relationship was demonstrated between the moyamoya vessels and perivascular hemosiderin deposition particularly around the subependymal-leptomeningeal anastomosis, suggesting the mechanism for the development of cMBs in MMD. Present study further supports previous findings that cMBs potentially serve as a marker for the bleeding- prone microangiopathy in MMD. The significance of the present study lies in selecting optimal surgical candidate for preventing future hemorrhage by the presence of the cMBs, whereas current surgical indication relying on the degree of ischemia frequently fails to detect patients with future hemorrhage. (C) 2014 by National Stroke Association
  • Masaki Ito, Yoshimasa Niiya, Haruto Uchino, Naoki Nakayama, Shoji Mabuchi, Kiyohiro Houkin
    WORLD NEUROSURGERY 82 1-2 2014年07月 [査読有り][通常論文]
     
    OBJECTIVE: Although the healing response after carotid balloon injury and carotid patch angioplasty injury has been well-documented in animal models, there is limited information about this process after carotid endarterectomy (CEA) in human patients. CASE DESCRIPTION: We describe the autopsy results of a 79-year-old man who died 18 days after CEA with patch angioplasty. The treated carotid artery had an adequate luminal diameter. Elastica-Masson staining revealed that the treated portion was covered with neointima but the patch graft was exposed to the arterial lumen at 18 days after CEA. Immunohistochemistry staining for alpha-smooth muscle actin (alpha-SMA), von-Willebrand factor, and vascular endothelial growth factor receptor-2 revealed that the neointima was mainly composed of alpha-SMA-positive cells. In addition, the alpha-SMA-rich neointima had many more Ki-67-positive cells than did the internal carotid artery intima in the area beyond the CEA-treated portion. CONCLUSIONS: This case report is the first to describe an entire carotid artery specimen in the acute stage after CEA with patch angioplasty. These findings suggest that an alpha-SMA-rich neointima with prominent proliferative activity covers the inner surface of the treated carotid artery, but patch grafts are left uncovered. The intrinsic arterial wall may have an important role in intimal regeneration after CEA, although the nature of the neointima and the mechanism by which it regulates proliferative activity remain unclarified.
  • Shigeru Yamaguchi, Shunsuke Terasaka, Hiroyuki Kobayashi, Katsuyuki Asaoka, Hiroaki Motegi, Hiroshi Nishihara, Hiromi Kanno, Rikiya Onimaru, Yoichi M. Ito, Hiroki Shirato, Kiyohiro Houkin
    PLOS ONE 9 5 e97108  2014年05月 [査読有り][通常論文]
     
    Background: Radiotherapy for high-grade meningioma (HGM) is one of the essential treatment options for disease control. However, appropriate irradiation timing remains under debate. The object of this study is to discern which prognostic factors impact recurrence in HGM patients and to propose a risk-stratification system for the application of postoperative radiotherapy.Methods: We retrospectively reviewed 55 adult patients who were diagnosed with Grade II and III intracranial meningioma. Cox regression models were applied to the analysis for impact on early recurrence in HGM patients without postoperative radiotherapy.Results: Grade III malignancy (P = 0.0073) and transformed histology (P = 0.047) proved to be significantly poor prognostic factors of early recurrence by multivariate analysis. The other candidates for recurrence factors were Simpson Grade 3-5 resection, preoperative Karnofsky Performance status, <= 70%, and MIB-1 labeling index >= 15%. According to these prognostic factors, postoperative HGM patients could be stratified into three recurrence-risk groups. The prognoses were significantly different between each group, as the 3-year actual recurrence-free rates were 90% in low-risk group, 31% in intermediate-risk group, and 15% in high-risk group.Conclusion: We propose recurrence-risk stratification for postoperative HGM patients using clinically available factors. Our results suggest that the prognosis for patients with high-risk HGMs is dismal, whereas HGM patients belonging to the low-risk group could have favorable prognoses. This stratification provides us with the criteria necessary to determine whether to apply adjuvant radiotherapy to postoperative HGM patients, and to also help identify potentially curable HGMs without adjuvant radiotherapy.
  • Takeo Abumiya, Masahito Katoh, Takuya Moriwaki, Masami Yoshino, Takeshi Aoki, Hiroyuki Imamura, Toshimitsu Aida, Hideo Scichinohe, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 23 5 896 - 901 2014年05月 [査読有り][通常論文]
     
    It is important to predict the outcome of tissue plasminogen activator (tPA)-treated patients early after the treatment for considering the post-tPA treatment option. We assessed cerebral blood flow (CBF) of tPA-treated patients with single-photon emission computed tomography (SPECT) 1 hour after tPA infusion to predict the patient outcome. Technetium-99m-hexamethylpropyleneamine oxime SPECT was performed in 35 consecutive tPA-treated patients. Asymmetry index, a contralateral-to-ipsilateral ratio of CBF, was calculated to analyze CBF quantitatively. Hypoperfusion or hyperperfusion was defined as a decrease of 25% or more or a increase of 25% or more in asymmetry index, respectively. Of all 35 patients, 23 had only hypoperfusion, 8 had both hypoperfusion and hyperperfusion, 2 had only hyperperfusion, and 2 had no perfusion abnormality. When evaluating the association between hypoperfusion and outcome, hypoperfusion volumes were significantly correlated with the modified Rankin Scale at 3 months (r = .634, P < .001). Hyperperfusion was observed in 10 patients (28.6%) and they showed a marked National Institutes of Health Stroke Scale score improvement in the first 24-hour period, which were significantly greater than those of 25 patients without hyperperfusion (P = .033). Eight patients (22.9%) with intracerebral hemorrhage (ICH) were all asymptomatic. Most ICHs were located in hypoperfusion areas, and no ICH was related to hyperperfusion. The results of the present study demonstrated that hypoperfusion volume was associated with poor outcome, whereas the presence of hyperperfusion seemed to be predictive of symptom improvement but not of development of ICH. Taken together, early post-treatment SPECT imaging seems to be a useful biomarker of outcome in tPA-treated patients.
  • Tamio Ito, Hiromi Kanno, Ken-ichi Sato, Mitsuteru Oikawa, Yoshimaru Ozaki, Hirohiko Nakamura, Shunsuke Terasaka, Hiroyuki Kobayashi, Kiyohiro Houkin, Kanako Hatanaka, Jyun-ichi Murata, Shinya Tanaka
    WORLD NEUROSURGERY 81 5-6 783 - 789 2014年05月 [査読有り][通常論文]
     
    OBJECTIVE: Pineal parenchymal tumors of intermediate differentiation (PPTID) are extremely rare tumor entities, and only limited data are available regarding their pathologic features and biologic behaviors. Because grading criteria of pineal parenchymal tumors (PPTs) have yet to be established, the treatment strategy and prognosis of PPTIDs remain controversial. We describe the clinicopathologic study of six patients with PPTID and compare responses for the treatment and prognosis with cases of pineocytoma (PC) and pine-oblastoma (PB). From this analysis, we attempt to clarify the treatment strategy for PPTIDs. METHODS: This study included 15 patients with PPTs, consisting of 6 PCs, 6 PPTIDs, and 3 PBs. We focused on the 6 patients with PPTIDs. All PPTID cases were treated surgically, and radiotherapy and chemotherapy were administered as adjuvant therapies in some cases. We have earlier reported the histopathologic study (Neuropathology 32: 647-653, 2012). Briefly, we examined mitotic figures and necrosis by hematoxylin-eosin staining and immunohistochemical markers such as neuronal markers (synaptophysin, neurofilament (NF), and neuronal nuclear antigen), and an MIB-1 labeling index was determined. RESULTS: In the PPTID cases, the extent of resection was variable and the recurrence rates among patients varied according to stage and treatment. All PC patients underwent total resection with no recurrence. All PB patients underwent resection and adjuvant therapy with radiotherapy and chemotherapy. There were no recurrences in patients with PC or PB. The results of histopathologic findings have been already reported as mentioned above. Briefly, the results indicated no mitotic figure or necrosis in any of the six cases of PPTID, but those features were observed in PB cases. All cases even including PC and PB were immunopositive for neuronal markers. The MIB-1 labeling index of PPTID was 3.5%, whereas it was 0% in PC and 10.5% in PB. CONCLUSIONS: Good radiosensitivity of PPTIDs was observed in our series. Because there are cases with discrepancies between images and pathologic findings, it is very difficult to determine the proper treatment strategy for PPTIDs. Proliferative potential was correlated with World Health Organization grade, although the immunoreactivity of neuronal markers did not correlate with the histologic grade.
  • Kota Kurisu, Hiroaki Motegi, Toshiya Osanai, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
    Case reports in neurology 6 2 207 - 12 2014年05月 [査読有り][通常論文]
     
    BACKGROUND: The mechanism by which acquired dural arteriovenous fistula (dAVF) develops is still unclear. Few cases have been reported with both dAVF and intracranial tumors, and in these few cases the authors have proposed that induced venous hypertension may lead to the pathogenesis of dAVF. We experienced a case of intrasinusoidal hemangiopericytoma (HPC) with dAVF development. In addition to its rare pathology and tumor location, this case showed regression of dAVF immediately after tumor removal. CASE REPORT: The patient was a 23-year-old man who developed progressively worse headaches and papilledema. The HPC was located entirely inside the confluence of the sinuses (CoS) and resulted in venous sinus occlusion. Cerebral angiography demonstrated a dAVF located in the straight sinus, upstream of the occluded CoS, which was fed by the dural branch of the posterior cerebral artery. After the endovascular embolization of the tumor feeders, subsequent surgery included venous reconstruction in addition to tumor excision. Although the dAVF was not treated with an endovascular procedure or surgery, postoperative angiography revealed complete disappearance of the dAVF. CONCLUSION: We conclude that venous reconstructive surgery greatly contributed to the immediate regression of the dAVF. When planning the treatment strategy for such cases, it should be remembered that acquired dAVF may regress due to the normalization of venous hypertension.
  • Yukitomo Ishi, Naoki Nakayama, Hiroyuki Kobayashi, Shigeru Yamaguchi, Shunsuke Terasaka, Kiyohiro Houkin
    Case Reports in Neurology 6 2 139 - 143 2014年04月16日 [査読有り][通常論文]
     
    Gastrointestinal stromal tumors (GISTs) are rare mesenchymal neoplasms arising from the gastrointestinal tract. The authors present a case of the successful removal of a metastatic GIST in the craniovertebral junction, using an occipital artery to posterior inferior cerebellar artery (OA-PICA) bypass. The patient is a 54-year-old male who underwent his first surgery for a small-bowel tumor at the age of 45 and was diagnosed with GIST. Nine years after his primary diagnosis, the patient suffered from severe neck pain. MRI demonstrated a large demarcated mass adjacent to the right atlas. The right vertebral artery (VA), completely engulfed by the tumor, showed a narrowing and ended in the PICA. Poor collateral blood supply in the right PICA territory was presumed. To prevent ischemic complications, an OA-PICA bypass was performed prior to the tumor resection. After the OA-PICA bypass, the tumor associated with the right VA was successfully removed, and the patient was discharged without any neurological deficits.
  • Toru Sasamori, Kazutoshi Hida, Kimio Anzai, Shunsuke Yano, Yasutaka Kato, Shinya Tanaka, Hisatoshi Saito, Kiyohiro Houkin
    CLINICAL IMAGING 38 2 199 - 201 2014年03月 [査読有り][通常論文]
     
    The authors reported a case of cervical juxtafacet cyst with extensive rim enhancement on gadolinium-diethylenetriamine pentaacid magnetic resonance imaging. Operative finding revealed the epidural space around the mass filled with abundant venous plexus. Histological examination demonstrated that cyst wall was composed of the well-vascularized fibrous connective tissue with some inflammatory changes. We speculate that extensive rim enhancement of juxtafacet cyst may be attributed not only to the chronic inflammatory changes of cyst wall, but to engorged venous plexus within the widened epidural space. (c) 2014 Elsevier Inc. All rights reserved.
  • Daisuke Shimbo, Takeo Abumiya, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
    BRAIN RESEARCH 1554 59 - 66 2014年03月 [査読有り][通常論文]
     
    Despite successful revascularization, reperfusion after prolonged ischemia causes ischemia reperfusion (I/R) injury. Recruitment and activation of neutrophils is thought to be a key event causing I/R injury. We examined whether post-ischemic intra-arterial infusion of liposome-encapsulated hemoglobin (LEH), an artificial oxygen carrier without neutrophils, could reduce I/R injury in a rat transient middle cerebral artery occlusion (MCAO) model. Male Sprague-Dawley rats were subjected to 2-h MCAO and then were divided into three groups: (1) LEH group (n=7) infused with LEH (Hb concentration of 6 g/dl, 10 ml/kg/h) through the recanalized internal carotid artery for 2 h, (2) vehicle group (n=8) infused with saline (10 ml/kg/h) in the same manner as the LEH group, and (3) control group (n=9) subjected to recanalization only. After 24-h reperfusion, all rats were tested for neurological score and then sacrificed to examine infarct and edema volumes, myeloperoxidase (MPO) expression, matrix metalloproteinase-9 (MMP-9) expression and activity, and reactive oxygen species (ROS) production. Compared with the control group and the vehicle group, the LEH group showed a significantly better neurological score and significantly smaller infarct and edema volumes. MPO expression, MMP-9 expression and activity, and ROS production in the LEH group were also significantly lower than those in the control and vehicle groups. The results in the present study suggest that post-ischemic intra-arterial infusion of LEH can reduce I/R injury through reducing the effect of MMP-9, most likely produced by neutrophils. This therapeutic strategy may be a promising candidate to prevent I/R injury after thrombolysis and/or thromboectomy. (C) 2014 Elsevier B.V. All rights reserved.
  • Ken Kazumata, Naoki Nakayama, Toshitaka Nakamura, Hiroyasu Kamiyama, Shunsuke Terasaka, Kiyohiro Houkin
    NEUROSURGERY 10 1 66 - 72 2014年03月 [査読有り][通常論文]
     
    BACKGROUND: Blood blister-like aneurysms (BBAs) are aneurysms with ill-defined fragile necks arising from an internal carotid artery (ICA) and associated with high mortality. OBJECTIVE: To describe strategies and outcomes in patients in whom radial artery (RA) graft bypass with ICA sacrifice was considered as the primary treatment during the acute phase of subarachnoid hemorrhage. METHODS: The authors analyzed the clinical records of 20 patients who were treated between 2004 and 2011 at their hospital and affiliate institutions. RESULTS: A majority of the patients were treated during the acute phase (< 24 hours, n = 15). A favorable outcome was achieved in 18 (90%) patients. The treatment strategies used were as follows: (1) ICA trapping/external carotid artery (ECA)-RA-middle cerebral artery (MCA) bypass (n = 13), (2) ICA trapping/superficial temporal artery-MCA bypass (n = 2), (3) aneurysm clipping with RA-MCA temporary bypass (n = 3), (3) aneurysm clipping with proximal ICA ligation and ECA-RA-MCA bypass (n = 1), and (4) direct clipping (n = 1). Postoperative infarction was observed in 6 patients and was ascribed to vasospasm (n = 1), retrograde thrombosis associated with trapping (n = 2), and reasons unrelated to the surgical procedures (n = 3). CONCLUSION: Trapping with RA graft bypass demonstrated favorable results in patients with internal carotid BBAs. Although trapping/RA graft bypass is a definitive treatment for BBAs located proximal to the origin of the posterior communicating artery, some distal BBAs preclude ICA trapping to spare the perforating arteries.
  • Masayuki Gekka, Shigeru Yamaguchi, Ken Kazumata, Hiroyuki Kobayashi, Hiroaki Motegi, Shunsuke Terasaka, Kiyohiro Houkin
    Case Reports in Neurology 6 1 68 - 73 2014年 [査読有り][通常論文]
     
    Here, we present a case of dorsal medulla oblongata hemangioblastoma with fourth ventricular hemorrhage. A 23-year-old female developed sudden consciousness disturbance, and CT revealed hemorrhage in all cerebral ventricles and a hyperdense mass in the cisterna magna. Although the reddish tumor located in the dorsal medulla oblongata was successfully removed, she suffered from severe tako-tsubo cardiomyopathy (TTC) and neurogenic pulmonary edema (NPE) because of baroreflex failure and damage to the solitary tract nuclei. After intensive care for 12 weeks following surgery, she was discharged without any neurological or radiological deficits. Pathogenesis of TTC/NPE is discussed in this paper. © 2014 S. Karger AG, Basel.
  • 伊師雪友, 青山剛, 栗栖宏多, 飛騨一利, 寳金清博
    Neurological Surgery 42 5 467 - 472 2014年 [査読有り][通常論文]
     
    An intradural arachnoid cyst is a relatively rare condition, occurring within the spinal subarachnoid space. We present the even-more rare case of an intradural arachnoid cyst associated with syringomyelia at the same spinal level. The patient was a 66-year-old man who presented with bilateral leg numbness and gait disturbance. Magnetic resonance imaging (MRI) revealed an intradural arachnoid cyst located dorsal to, and compressing, the thoracic spinal cord at the level of the 7th thoracic vertebra (Th 7). In addition, syringomyelia existed at the level of Th 8, slightly caudal to the intradural arachnoid cyst. We dissected the cyst but did not perform any surgical procedures for the syringomyelia. Post-operative MRI showed that the cyst had disappeared and the syringomyelia had spontaneously shrunk. The patient was discharged with improvement in his numbness and gait disturbance. There are a few case reports of intradural arachnoid cysts associated with syringomyelia, but recent evidence suggests that its occurrence is more common than previously thought. A combination of these two diseases is thought to be caused by blockage of cerebrospinal fluid (CSF) flow, which is also thought to cause adhesive arachnoiditis. For this reason, resection of the arachnoid cyst could improve the CSF flow and contribute to the shrinkage of syringomyelia. Furthermore, early treatment may correlate with improvement in radiological findings and neurological symptoms.
  • Satoshi Kuroda, Masahito Kawabori, Kenji Hirata, Tohru Shiga, Daina Kashiwazaki, Kiyohiro Houkin, Nagara Tamaki
    ACTA NEUROCHIRURGICA 156 1 77 - 83 2014年01月 [査読有り][通常論文]
     
    Even after the recent randomized clinical trials JET and COSS, it is still unclear that impaired cerebrovascular reactivity (CVR) to acetazolamide and oxygen extraction fraction (OEF) can identify the candidates for superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis. This prospective study was aimed to evaluate the benefits of STA-MCA "double" anastomosis on long-term outcome in patients with reduced cerebral blood flow (CBF) and CVR (Type 3 ischemia) and elevated OEF attributable to occlusive carotid diseases. This study included 49 patients with reduced CBF and CVR on SPECT in the ipsilateral MCA area. Using O-15-gas PET, OEF was also measured in all patients. STA-MCA double anastomosis was recommended to the patients with Type 3 and elevated OEF. Those with Type 3 but normal OEF were medically treated. Of 36 patients with Type 3 and elevated OEF, 25 consented to surgery. No perioperative morbidity or mortality were noted. The other 11 patients with Type 3 and elevated OEF were medically treated. Annual incidence of ipsilateral stroke was 0.7 % and 6.5 % in surgically and medically treated patients with Type 3 and elevated OEF, respectively (P = 0.0188). None of patients with Type 3 but normal OEF developed ipsilateral stroke during follow-up periods. STA-MCA "double" anastomosis significantly decreased OEF. STA-MCA "double" anastomosis may still have the potential to reduce the risk of recurrent ipsilateral stroke in hemodynamically compromised patients. Further studies would be essential to advance diagnosis, surgical procedures, and perioperative managements to bring out maximal effects of bypass surgery.
  • Daina Kashiwazaki, Rina Kobayashi, Kiyohiro Houkin, Satoshi Kuroda
    BRITISH JOURNAL OF NEUROSURGERY 28 1 119 - 121 2014年01月 [査読有り][通常論文]
     
    Case. A 24-year-old man with Spetzler-Martin (S-M) Grade III arteriovenous malformation (AVM). He was conservatively treated, but AVM significantly increased in size over 5 years, judged as Grade IV. Subsequently, he developed intracerebral hemorrhage. AVM was totally removed. Immunohistochemistry showed that the endothelial cells in the dura and nidus were positive for VEGF and VEGF-R.
  • 譚成博, 七戸秀夫, 寳金清博
    再生医療 13 2014年
  • 七戸秀夫, 寳金清博
    血液事業 36 4 2014年
  • 笹森徹, 関俊隆, 矢野俊介, 飛騨一利, 齋藤久壽, 寳金清博
    日本脊髄外科学会プログラム・抄録集 29th 2014年
  • 伊師雪友, 関俊隆, 飛騨一利, 笹森徹, 寳金清博
    日本脊髄外科学会プログラム・抄録集 29th 2014年
  • 東海林菊太郎, 東海林菊太郎, 飛騨一利, 関俊隆, 矢野俊介, 寳金清博
    日本脊髄外科学会プログラム・抄録集 29th 2014年
  • 笹森徹, 関俊隆, 矢野俊介, 飛騨一利, 齋藤久壽, 寳金清博
    日本脊髄外科学会プログラム・抄録集 29th 2014年
  • 七戸秀夫, 寳金清博, 黒田敏
    日本磁気共鳴医学会雑誌 34 2 2014年
  • 七戸秀夫, 寳金清博
    神経治療学 31 3 2014年
  • 七戸秀夫, 寳金清博
    再生医療 13 2014年
  • 七戸秀夫, 寳金清博, 黒田敏
    日本脳循環代謝学会総会プログラム・抄録号 26th 2014年
  • 譚成博, 七戸秀夫, 鐙谷武雄, 中山若樹, 数又研, 穂刈正昭, 浜内祝嗣, 寳金清博
    日本脳循環代謝学会総会プログラム・抄録号 26th 2014年
  • 寳金清博
    新規医薬品・医療機器等の創出、難治性疾患の治療法の開発および最適な治療法の確立に関する研究 平成25年度 総括・分担研究報告書 2014年
  • 七戸秀夫, 寳金清博
    日本臨床 72 2014年
  • 遠藤将吾, 寺坂俊介, 茂木洋晃, 寳金清博
    小児の脳神経 38 6 2014年
  • 七戸秀夫, 鐙谷武雄, 中山若樹, 数又研, 穂刈正昭, 譚成博, 寳金清博, 黒田敏
    日本脳循環代謝学会総会プログラム・抄録号 26th 2014年
  • Sandra Vuignier, Masaki Ito, Kota Kurisu, Ken Kazumata, Naoki Nakayama, Hideo Shichinohe, Tohru Shiga, Jozsef Zoltan Kiss, Nagara Tamaki, Kiyohiro Houkin
    Acta Neurochirurgica 155 11 2097 - 2104 2013年11月 [査読有り][通常論文]
     
    Background: The prevalence of ivy sign on fluid-attenuated inversion recovery (FLAIR) imaging in patients with asymptomatic moyamoya disease is unclear. The aim of this study was to clarify the incidence of ivy sign in these patients, as well as the correlation between MRI and 15O gas PET findings. Methods: A retrospective analysis including 16 consecutive patients with asymptomatic moyamoya disease enrolled between 2001 and 2010 in a single center. FLAIR imaging at the initial visit was categorized as ivy sign present, negative, or equivocal. Hemodynamic and metabolic parameters were quantified in 11 of 16 patients by 15O-gas positron emission tomography, and the relationship between ivy sign and 15O-gas PET parameters was analyzed. Cerebrovascular events within the follow-up period (54 ± 28 months) were also examined. Results: Five of 16 asymptomatic moyamoya patients (31.3 %) had positive ivy sign (6/30 hemispheres, 20 %). In 15O-gas PET examinations, 18 % of 22 hemispheres had elevated oxygen extraction fraction values that were significantly associated with positive ivy sign. Of these 16 asymptomatic moyamoya patients, six patients (37.5 %) underwent combined surgical revascularization. In this series, no patients experienced ischemic stroke, but one had intraventricular bleeding 1 year after surgery. Conclusions: Ivy sign on FLAIR imaging is still not rare in patients with moyamoya disease, even when asymptomatic. Although optimal management is still under debate, ivy sign may be an indicator of misery perfusion, and patients with asymptomatic moyamoya disease and ivy sign on FLAIR imaging will benefit from more careful follow-up. © 2013 Springer-Verlag Wien.
  • Hiroyuki Kobayashi, Kenji Hirata, Shigeru Yamaguchi, Shunsuke Terasaka, Tohru Shiga, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 53 11 773 - 778 2013年11月 [査読有り][通常論文]
     
    Glioma is one of the most common brain tumors in adults. Its diagnosis and management have been determined by histological classifications. It is difficult to establish new paradigms because the pathology has matured and a great deal of knowledge has accumulated. On the other hand, we understand that there are limitations to this gold-standard because of the heterogeneity of glioma. Thus, it is necessary to find new criteria independent of conventional morphological diagnosis. Molecular imaging such as positron emission tomography (PET) is one of the most promising approaches to this challenge. PET provides live information of metabolism through the behavior of single molecules. The advantage of PET is that its noninvasive analysis does not require tissue sample, therefore examination can be performed repeatedly. This is very useful for capturing changes in the biological nature of tumor without biopsy. In the present clinical practice for glioma, F-18-fluorodeoxyglucose (FDG) PET is the most common tracer for predicting prognosis and differentiating other malignant brain tumors. Amino acid tracers such as C-11-methionine (MET) are the most useful for detecting distribution of glioma, including low-grade. Tracers to image hypoxia are under investigation for potential clinical use, and recently, F-18-fluoromisonidazole (FMISO) has been suggested as an effective tracer to distinguish glioblastoma multiforme from others.
  • Masahito Kawabori, Satoshi Kuroda, Naoki Nakayama, Kenji Hirata, Toru Shiga, Kiyohiro Houkin, Nagara Tamaki
    WORLD NEUROSURGERY 80 5 612 - 619 2013年11月 [査読有り][通常論文]
     
    BACKGROUND: Headache is one of the major clinical presentations in pediatric Moyamoya disease. However, the clinical features and underlying mechanisms are not fully understood. This study aimed to clarify the clinical feature of headache in pediatric Moyamoya disease and the effect of surgical revascularization. METHODS: This study included 29 pediatric patients who underwent superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis and indirect bypass for Moyamoya disease. Their medical records were precisely evaluated to identify the clinical features of their headache. The findings on magnetic resonance imaging, positron emission tomography, and single-photon emission computed tomography also were analyzed. RESULTS: Preoperative headache was documented in 11 (38%) of 29 patients. The majority of them complained of severe headache in the frontal or temporal region in the morning. Headache was significantly related to more advanced disease stage and to the decreases in cerebral blood flow and its reactivity to acetazolamide. Surgical revascularization completely resolved headache in all 11 patients. CONCLUSIONS: These findings strongly suggest that disturbed cerebral hemodynamics may play key roles in developing severe headache in pediatric Moyamoya disease. STA-MCA anastomosis and encephalo-duro-myo-arterio-pericranial synangiosis may be effective procedures to rapidly resolve headache by widely supplying collateral blood flow to the operated hemispheres.
  • Hisayasu Saito, Keiichi Magota, Songji Zhao, Naoki Kubo, Yuji Kuge, Hideo Shichinohe, Kiyohiro Houkin, Nagara Tamaki, Satoshi Kuroda
    STROKE 44 10 2869 - 2874 2013年10月 [査読有り][通常論文]
     
    Background and Purpose This study was aimed to assess whether I-123-iomazenil (IMZ) single photon emission computed tomography can serially monitor the effects of bone marrow stromal cell (BMSC) transplantation on neuronal integrity in infarct brain of rats. Methods The BMSCs were harvested from green fluorescent protein-transgenic rats and were cultured. The rats were subjected to permanent middle cerebral artery occlusion. Their motor function was serially quantified throughout the experiments. The BMSCs or vehicle was stereotactically transplanted into the ipsilateral striatum at 7 days after the insult. Using small-animal single photon emission computed tomography/computed tomography apparatus, the I-123-IMZ uptake was serially measured at 6 and 35 days after the insult. Finally, fluorescence immunohistochemistry was performed to evaluate the distribution of engrafted cells and their phenotypes. Results The distribution of I-123-IMZ was markedly decreased in the ipsilateral neocortex at 6 days postischemia. The vehicle-transplanted animals did not show a significant change at 35 days postischemia. However, BMSC transplantation significantly improved the distribution of I-123-IMZ in the peri-infarct neocortex as well as motor function. The engrafted BMSCs were densely distributed around cerebral infarct, and some of them expressed neuronal nuclear antigen and -aminobutyric acid type-A receptor. Conclusions The present findings strongly suggest that the BMSCs may enhance functional recovery by improving the neuronal integrity in the peri-infarct area, when directly transplanted into the infarct brain at clinically relevant timing. I-123-IMZ single photon emission computed tomography may be a promising modality to scientifically prove the beneficial effects of BMSC transplantation on the host brain in clinical situation.
  • Masaaki Hokari, Satoshi Kuroda, Naoki Nakayama, Kiyohiro Houkin, Tatsuya Ishikawa, Hiroyasu Kamiyama
    NEUROSURGICAL REVIEW 36 4 567 - 571 2013年10月 [査読有り][通常論文]
     
    We retrospectively investigated surgical immediate and long-term overall results after clipping of the unruptured aneurysms. Between 1991 and 2008, 166 patients underwent neck clipping of unruptured saccular aneurysms at our institute. Patients were subsequently followed to clarify the occurrence of subarachnoid hemorrhage (SAH), and stroke other than SAH, aneurysm recurrence, cerebrovascular death, all-cause death, and risk factors. Surgical complication was noted in 14 patients (8.4 %) and surgical morbidity in two patients (1.2 %). Of 164 patients except for these two patients who suffered surgical morbidity, we could obtain more than 3 years follow-up information for 144 patients (87.8 %). There were 49 men and 95 women. The mean age was 58.5 years, and mean follow-up period was 7.9 years. Eight cases had died during follow-up (hepatic insufficiency in one, renal insufficiency in one, suicide in one, intracerebral hemorrhage (ICH) in two, SAH in one, and pneumonia after stroke in two). Therefore, the cause of death was stroke and late effects of stroke. Twelve symptomatic cerebrovascular events (cerebral infarction in seven, ICH in four, and SAH in one) occurred in ten patients. Consequently, annual risk of SAH after clipping of unruptured aneurysms was 0.085 %. Besides, annual risk of stroke in those patients was 1.06 %, and this incidence was higher than that in the general population. Although this study confirmed the good surgical result, annual risk of stroke after clipping of unruptured aneurysms was much higher than that in the general population. The long-term periodic examination to detect recurrent aneurysms and appropriate management to prevent stroke should be performed for patients with surgically treated unruptured aneurysm.
  • Chang Il Ju, Kazutoshi Hida, Tomohiro Yamauchi, Kiyohiro Houkin
    JOURNAL OF KOREAN NEUROSURGICAL SOCIETY 54 3 257 - 260 2013年09月 [査読有り][通常論文]
     
    A 61-year-old woman with a very rare case of totally ossified large thoracic spinal metaplastic meningioma, showing progressing myelopathy is presented. Computed tomographic images showed a large totally ossfied intradural round mass occupying the spinal canal on T9-10 level. Magnetic resonance imaging revealed a large T9-10 intradural extramedullary mass that was hypointense to spinal cord on T1- and T2-weighted sequences, partial enhancement was apparent after Gadolinium administration. The spinal cord was severely compressed and displaced toward the right at the level of T9-10. Surgical removal of the tumor was successfully accomplished via the posterior midline approach and the histological diagnosis verified an ossified metaplastic meningioma. The clinical neurological symptoms of patient were improved postoperatively. In this article we discuss the surgical and pathological aspects of rare case of spinal totally ossified metaplastic meningioma.
  • Takeshi Mikami, Toshiya Sugino, Shunya Ohtaki, Kiyohiro Houkin, Nobuhiro Mikuni
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 22 6 862 - 8 2013年08月 [査読有り][通常論文]
     
    Flow voids in the basal ganglia cannot always be recognized on magnetic resonance imaging, even in patients with typical moyamoya disease. In this report, flow voids in the basal ganglia and cisternal flow voids of the sylvian valley were evaluated in patients with moyamoya disease, and their diagnostic value was verified. A total of 41 consecutive patients with moyamoya disease were included in this analysis. The number of flow voids in the basal ganglia and the sylvian valley were counted on each side by 3 observers. Then the numbers of flow voids were compared between the patients with moyamoya disease and controls. The patients with moyamoya disease had a significantly higher mean number of flow voids in the basal ganglia and the sylvian valley (P < .0001); however, the number of flow voids in the basal ganglia was 0 or 1 in 69 sides (28.0%) in patients with moyamoya disease. Comparative analysis using the area under the receiver operating curve indicated that the evaluation of flow voids in the sylvian valley was significantly superior method to that in the basal ganglia (P < .0001). The cutoff value for the number of cisternal flow voids in the sylvian valley for the diagnosis of moyamoya disease was 6. Based on these findings, we recommend a definitive diagnosis of moyamoya disease should include assessment for abnormal vessels around the terminal portions of the internal carotid arteries.
  • Toshiya Sugino, Takeshi Mikami, Kei Miyata, Kengo Suzuki, Kiyohiro Houkin, Nobuhiro Mikuni
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 22 6 811 - 816 2013年08月 [査読有り][通常論文]
     
    Arterial spin labeling (ASL) magnetic resonance imaging (MRI) is a technique for depicting cerebral perfusion without contrast medium. The purpose of this study was to determine whether ASL can be used to detect hyperperfusion after revascularization for moyamoya disease as effectively as N-isopropyl-[123I]beta-iodoamphetamine (I-123-IMP) single-photon emission computed tomography (SPECT). Fifteen consecutive patients with moyamoya disease were included in the study. All patients underwent surgical revascularization. Postoperatively, regional cerebral blood flow (rCBF) was measured by flow-sensitive alternating inversion recovery (FAIR) ASL and I-123-IMP SPECT during the acute stage, and rCBF of the operative side was compared with the other side. The asymmetry ratio (AR) was then calculated from the rCBF as measured using each modality. The postoperative AR of ASL was moderately correlated with that of I-123-IMP SPECT (y = 0.180x 1 0.819; R = 0.80; P = .0003). In this series, 2 patients (13.3%) suffered symptomatic hyperperfusion after revascularization and accordingly exhibited increased AR of ASL. Our data indicate that early increases in rCBF in patients with hyperperfusion could be detected using FAIR ASL supplemental to I-123-IMP SPECT after revascularization. Our data indicate that FAIR ASL is a convenient method for evaluating hyperperfusion that can be performed repeatedly without the use of contrast medium or radioisotopes.
  • Toshiya Sugino, Takeshi Mikami, Shunya Ohtaki, Tohru Hirano, Satoshi Iihoshi, Kiyohiro Houkin, Nobuhiro Mikuni
    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 22 5 644 - 9 2013年07月 [査読有り][通常論文]
     
    The recent introduction of multidetector row computed tomography (MDCT) scanners has enabled high-resolution 3-dimensional reconstruction. The purpose of this study was to establish a method to evaluate moyamoya disease using computed tomography angiography (CTA), specifically MDCT. Twenty-four patients (48 sides total) with moyamoya disease diagnosed by magnetic resonance angiography (MRA) were evaluated by means of CTA using MDCT by 3 independent observers, and the resulting 144 sides were analyzed. CTA and MRA were compared in terms of the steno-occlusive changes exhibited in each vessel. CTA and MRA scores were assigned on the basis of the severity of occlusive changes in the internal carotid artery, middle cerebral artery, anterior cerebral artery, and posterior cerebral artery. CTA scores were significantly correlated with MRA scores (P < .0001), and the 2 scores were in complete agreement in 57 sides (39.6%). The mean CTA score was significantly lower than the mean MRA score (P < .0001). Compared with CTA, MRA overestimated occlusion in 115 of the 576 vessels assessed. The mean MRA score was significantly higher in the overestimation group than in the good correlation group (P < .0001). CTA had a significantly higher rate of detection of moyamoya-affected vessels (P = .0001). Our data indicate that CTA using MDCT is a more reliable technique than MRA for diagnosing moyamoya disease. The ability to perform CTA quickly is a significant benefit for patients with moyamoya disease, particularly in pediatric and emergency cases.
  • Shigeki Sumi, Hideki Origasa, Kiyohiro Houkin, Yasuo Terayama, Shinichiro Uchiyama, Hiroyuki Daida, Hiroshi Shigematsu, Shinya Goto, Kortaro Tanaka, Susumu Miyamoto, Kazuo Minematsu, Masayasu Matsumoto, Yasushi Okada, Motoki Sato, Norihiro Suzuki
    INTERNATIONAL JOURNAL OF STROKE 8 4 251 - 257 2013年06月 [査読有り][通常論文]
     
    Background The Essen stroke risk score is widely applied to predict the risk of recurrent ischemic stroke. We developed a modified Essen stroke risk score and validated it using a large prospective Effective Vascular Event REduction after STroke (EVEREST) registry including 3588 patients with ischemic stroke in Japan. Patients with cardioembolic stroke were excluded, and follow-up was one-year. Methods The modified Essen stroke risk score was calculated from scores for waist circumference, stroke subtype by etiology, and gender in addition to age, hypertension, diabetes mellitus, previous myocardial infarction, other cardiovascular diseases except myocardial infarction and atrial fibrillation, peripheral artery disease, smoking, and previous stroke or transient ischemic attack. A multiple logistic regression model identified the predictors (each assigned one or two points) and provided c-statistics for the modified Essen stroke risk score. We considered two outcomes, recurrent ischemic stroke and cardiovascular events (defined as the combined outcomes of fatal or nonfatal stroke, myocardial infarction, nonfatal unstable angina, and cardiac death). Results Recurrent ischemic stroke occurred in 121 patients (37%) and cardiovascular events occurred in 133 (40%) within a year. The c-statistic (used for discrimination) was 0632 for recurrent stroke and 0640 for cardiovascular events. Patients scoring 6 or greater were classified as high risk, otherwise were classified as low risk. KaplanMeier analysis revealed that the modified risk score was more predictive than the Essen stroke risk score in both men and women. Conclusions The modified Essen stroke risk score increased the ability of the Essen stroke risk score to predict recurrent cardiovascular events. Patients with a high modified Essen stroke risk score should be candidates for intensified secondary prevention strategies.
  • Shogo Endo, Hiroyuki Kobayashi, Shunsuke Terasaka, Akihiro Iguchi, Yuko Cho, Junjiro Ohshirma, Kanako Kubota, Kiyohiro Houkin
    CLINICAL NEUROLOGY AND NEUROSURGERY 115 6 811 - 813 2013年06月 [査読有り][通常論文]
  • Hisayasu Saito, Satoshi Kuroda, Shunsuke Terasaka, Takeshi Asano, Naoki Nakayama, Kiyohiro Houkin
    Case Reports in Neurology 5 2 135 - 138 2013年05月 [査読有り][通常論文]
     
    Objective: Isolated accessory nerve palsy due to intracranial disorders is uncommon because intracranial accessory nerve injury usually occurs in case of a skull base tumor or trauma, resulting in one of multiple cranial nerve palsies. We report a very rare case of isolated accessory nerve palsy due to a large thrombosed aneurysm of the intracranial vertebral artery. Full recovery was achieved after surgery. Case Report: A patient complaining of transient numbness in the right side was referred to our hospital. An MRI indicated a large thrombosed aneurysm of the right vertebral artery. The aneurysm severely compressed the medulla oblongata. First, the proximal vertebral artery (VA) was clipped with an aneurysm clip to reduce the pressure inside the aneurysm. However, cerebral angiography revealed a partial recanalization of the right VA. The patient then underwent coil embolization of the right VA just proximal to the aneurysm clip. Subsequently, the right VA was completely obliterated. The patient was discharged without any neurological deficit. Two weeks later, however, she complained of right shoulder pain. Physical and neurological examinations demonstrated atrophy of the right trapezius and sternocleidomastoid muscle, leading to a deepening of the right supraclavicular fossa. The symptoms were considered to result from the right isolated accessory nerve palsy. Follow-up MRI showed that the VA aneurysm gradually decreased in size over a period of several months. At the same time, her symptoms disappeared completely. Conclusion: We should keep in mind that isolated accessory nerve palsy can be caused by a large or giant vertebral aneurysm. © 2013 S. Karger AG, Basel.
  • Maiko Kawamura, Masanori Ishiguro, Takashi Nagamine, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 53 5 291 - 298 2013年05月 [査読有り][通常論文]
     
    Vasoconstriction of arteries induced by serotonin (5-hydroxytryptamine: 5-HT) is mediated by 5-HT2A and 5-HT1B receptors localized on smooth muscle. The present study investigated the impact of sarpogrelate, a 5-HT2A receptor antagonist, on cerebral artery diameter in the presence and absence of exogenous 5-HT. Diameter measurements were obtained in vitro from rabbit cerebral arteries pressurized to 60 mmHg. In the absence of 5-HT, arteries exhibiting pressure-induced myogenic tone dilated to sarpogrelate in a concentration-dependent manner (half maximal inhibitory concentration [IC50] approximate to 2.3 mu M). In a separate experimental series, exogenous application of 5-HT (0.01 mu M) caused further constriction of myogenically active arteries, decreasing cerebral artery diameter by an additional 25%. In the presence of 5-HT, sarpogrelate caused concentration-dependent vasodilation (IC50 approximate to 2.3 mu M) that was similar to that observed in the absence of exogenous 5-HT. Dilation induced by sarpogrelate was not affected by physical removal of the endothelium or inhibition of nitric oxide synthase with N omega-nitro L-arginine. The highest concentration of sarpogrelate (100 mu M) induced near maximal dilation, comparable to dilation induced by the L-type voltage-dependent calcium channel antagonist diltiazem. These findings suggest that in rabbit cerebral arteries, sarpogrelate has direct vasodilator effects on vascular smooth muscle.
  • Motoyuki Iwasaki, Kazutoshi Hida, Takeshi Aoyama, Kiyohiro Houkin
    EUROPEAN SPINE JOURNAL 22 S317 - S320 2013年05月 [査読有り][通常論文]
     
    Background An intramedullary subependymoma is rare, particularly in the thoracolumbar region. Moreover, a radiographical obvious cystic formation of subependymoma of spinal cord rarely occurs in comparison to ependymoma. Case report A 57-year-old woman presented with paraparesis. MRI revealed a multinodular and multicystic lesion in the spinal cord that was difficult to diagnose correctly. Intraoperative observation via midline myelotomy revealed a grayish, gelatinous solid mass with an eccentric localization. In addition, DREZtomy on the caudal side of the tumor revealed cystic formation. The cyst was punctured and xanthochromic fluid was collected. Attenuation of motor evoked potential (MEP) resulted in the partial removal of tumor. A pathological examination revealed the mass to be a subependymoma. The patient experienced transient worsening of symptoms, but improved gradually. No adjuvant radiosurgery was administered. Follow-up estimation 30 months after surgery revealed no evidences of regrowth. Conclusions This report presents this rare case, a review of the literature associated with thoracolumbar subependymomas, and a discussion of the clinical and radiographical characteristics.
  • Hiroaki Motegi, Yuuta Kamoshima, Shunsuke Terasaka, Hiroyuki Kobayashi, Shigeru Yamaguchi, Mishie Tanino, Junichi Murata, Kiyohiro Houkin
    BRAIN TUMOR PATHOLOGY 30 2 67 - 72 2013年04月 [査読有り][通常論文]
     
    The purpose of this study was to distinguish pseudoprogression (PP) from early true progression in patients with glioblastoma (GBM) based on the presence of a mutation in isocitrate dehydrogenase 1 (IDH1). We retrospectively surveyed 32 patients with GBM or GBM with oligodendroglioma component (GBMO) who underwent biopsy or maximal tumor resection followed by concurrent radiotherapy and temozolomide (TMZ). We then selected patients with early radiological progression in magnetic resonance imaging within 6 months after concurrent radiotherapy and TMZ treatment. DNA was extracted from their tumor blocks. The IDH1 mutation was analyzed in the genomic region by direct sequencing as a biomarker for PP. Twenty-eight patients were diagnosed with GBM and four with GBMO. Eleven patients were discovered to have early radiological progression. PP was detected in two patients (6.3 %) diagnosed with GBMO and one patient with GBM. Both of the GBMO patients with PP had the IDH1 mutation, the one GBM patient with PP and the other eight patients with early true progression with wild type. The sensitivity and specificity of the IDH1 mutation for detecting PP were 66.7 and 100 %, respectively. This study suggests the IDH1 mutation may become a novel molecular biomarker for PP. Analyzing the IDH1 mutation, in the case of recognizing early radiological progression, may enable distinction of PP from early true progression, and we could determine the need for second-look surgery.
  • Shogo Endo, Shunsuke Terasaka, Shigeru Yamaguchi, Hitoshi Ikeda, Tsutomu Kato, Hiroyuki Kobayashi, Shinya Tanaka, Kiyohiro Houkin
    NEUROPATHOLOGY 33 2 185 - 191 2013年04月 [査読有り][通常論文]
     
    In the CNS, primary tumors with rhabdoid components are classified as atypical teratoid/rhabdoid tumor, rhabdoid meningioma or rhabdoid glioblastoma. The authors present a young adult patient with supratentorial rhabdoid tumor incidentally found after head trauma as a small pre-existing lesion in the parahippocampal gyrus. MRI demonstrated an area of hypointensity on T1-weighted images and hyperintensity on T2-weighted and fluid attenuated inversion recovery images. A serial MR scan revealed no change 3 months after the initial examination but drastic changes at 6 months. As the tumor and accompanying intratumoral hemorrhage enlarged rapidly, resection of the tumor was performed. Histopathology revealed that the main component of the tumor was typical rhabdoid cells with some necrotic areas. There were also pathological features consistent with oligoastrocytoma. The specimen had neither vascular proliferation usually seen in high-grade glioma nor the meningothelial pattern that suggests meningioma. Immunohistochemical findings revealed that cells were strongly positive for vimentin, epithelial membrane antigen and INI-1 antibody throughout the specimen. Further, monosomy 22 was detected by fluorescence in situ hybridization. The tumor was finally thought to be an unclassifiable primitive rhabdoid tumor with oligoastrocytoma that arose in the CNS. The patient died within 5 months of detection of the tumor, regardless of surgical resection, radiotherapy and chemotherapy.
  • Masahito Kawabori, Satoshi Kuroda, Masaki Ito, Hideo Shichinohe, Kiyohiro Houkin, Yuji Kuge, Nagara Tamaki
    NEUROPATHOLOGY 33 2 140 - 148 2013年04月 [査読有り][通常論文]
     
    Stereotactic transplantation of bone marrow stromal cells (BMSCs) enables efficient delivery to the infarct brain. This study was aimed to assess its optimal timing and cell dose for ischemic stroke. The BMSCs were harvested from the green fluorescent protein-transgenic rats and were labeled with quantum dots. The BMSCs (1x105 or 1x106) were stereotactically transplanted into the ipsilateral striatum of the rats subjected to permanent middle cerebral artery occlusion at 1 or 4 weeks post-ischemia. Motor function was serially assessed. Using in vivo near infrared (NIR) fluorescence imaging, the engrafted BMSCs were visualized at 3 weeks post-transplantation. Immunohistochemistry was performed to evaluate their fate. Functional recovery was significantly enhanced when both low and high doses of BMSCs were transplanted at 1 week post-ischemia, but such therapeutic effects were observed only when the high-dose BMSCs were transplanted at 4 weeks post-ischemia. Both optical imaging and immunohistochemistry revealed their better engraftment in the peri-infarct area when the high-dose BMSCs were transplanted at 1 or 4 weeks post-ischemia. These findings strongly suggest the importance of timing and cell dose to yield therapeutic effects of BMSC transplantation for ischemic stroke. Earlier transplantation requires a smaller number of donor cells for beneficial effects.
  • Haruto Uchino, Toshitaka Nakamura, Kiyohiro Houkin, Jun-ichi Murata, Hisatoshi Saito, Satoshi Kuroda
    ACTA NEUROCHIRURGICA 155 4 599 - 605 2013年04月 [査読有り][通常論文]
     
    Postoperative hyperperfusion may lead to severe neurological complications after superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis. However, there are no reliable modalities to predict the occurrence of postoperative hyperperfusion during surgery. The purpose of this study is to evaluate whether a semiquantitative analysis of indocyanine green (ICG) videoangiography could be useful in predicting postoperative hyperperfusion after STA-MCA anastomosis. This study included seven patients who underwent STA-MCA anastomosis due to occlusive carotid artery diseases. During surgery, ICG videoangiography was performed before and after bypass procedures, and ICG intensity-time curves were semiquantitatively analyzed to evaluate hemodynamic changes by calculating maximum intensity, time to peak (TTP), and blood flow index (BFI). Maximum intensity significantly increased from 252.6 +/- 132.5 to 351.7 +/- 151.9 after bypass (p < 0.001). TTP was significantly shortened from 12.9 +/- 4.4 s to 9.8 +/- 3.7 s (p < 0.001). Furthermore, BFI significantly increased from 33.9 +/- 28.1 to 74.6 +/- 88.4 (p < 0.05). Postoperative hyperperfusion was observed in five of seven patients 1 day after surgery. The ratio of BFI before and after bypass procedures was significantly higher in patients with postoperative hyperperfusion than those without, 2.5 +/- 1.1 and 1.5 +/- 0.4, respectively (p = 0.013). These findings suggest that semiquantitative analysis of ICG videoangiography is helpful in predicting occurrence of hyperperfusion after STA-MCA anastomosis in patients with occlusive carotid artery diseases.
  • Takeshi Mikami, Yoshihiro Minamida, Yukinori Akiyama, Masahiko Wanibuchi, Toshiya Sugino, Kiyohiro Houkin, Nobuhiro Mikuni
    Neurosurgical review 36 2 303 - 8 2013年04月 [査読有り][通常論文]
     
    In patients with vascular compression syndromes, the preoperative recognition of the cranial nerves and compressed vessels will contribute to improved surgical results. The aim of this study was to clarify the pathophysiology of hemifacial spasm (HFS) associated with the vertebral artery (VA) and to assess the value of preoperative imaging. Fifty-three consecutive patients with HFS underwent microvascular decompression (MVD). Of these, 18 cases of HFS were associated with the VA; this case series was compared with the remaining non-VA-associated HFS. For preoperative assessment, magnetic resonance imaging (MRI) was performed. Since January 2006, fusion imaging has been performed by combining MRI and computed tomography angiography. Of the 18 cases of VA-associated HFS, 17 (94.4 %) were on the left side; this was significantly higher than in the non-VA-associated HFS cases (p < 0.0001). The VA was attached to the root exit zone (REZ) directly in three cases. In the other 15 cases, the VA was compressing the REZ indirectly as a result of other intervening vessels. In all cases, preoperative imaging fully simulated the status of the REZ. The symptom disappeared in 17 cases (94.4 %) after MVD, and there was no significant difference in the surgical results between the VA-associated group and the non-VA-associated group (p = 0.9925). HFS associated with VA is not a rare condition. Preoperative recognition is thus important to the improvement of surgical results. Fusion imaging is useful to determine the status of the REZ, especially in indirect VA-associated HFS.
  • 伊師 雪友, 寺坂 俊介, 茂木 洋晃, 栗栖 宏多, 小林 浩之, 寳金 清博, 山田 洋介, 畑中 佳奈子
    北海道医学雑誌 88 2-3 111 - 111 北海道医学会 2013年04月 [査読有り][通常論文]
  • 中山若樹, 寳金清博
    Clin Neurosci 31 4 408 - 410 2013年04月01日 [査読無し][通常論文]
  • 七戸秀夫, 黒田敏, 宮本倫行, 山内朋裕, 斎藤久泰, 新保大輔, 数又研, 中山若樹, 寶金清博
    再生医療 12 237  2013年02月28日 [査読無し][通常論文]
  • 大瀧隼也, 三上毅, 飯星智史, 宮田圭, 野中雅, 寳金清博, 三國信啓
    Neurological Surgery 41 2 107 - 115 2013年02月 [査読有り][通常論文]
     
    The treatment of a large-giant aneurysm in the cavernous portion of an internal carotid artery (ICA) requires occlusion of the ICA, with or without extracranial-intracranial (EC-IC) bypass surgery Although a balloon occlusion test (BOT) is used to determine the need for bypass surgery and select the bypass type, the criteria are not well established In this study, we retrospectively analyzed 10 consecutive patients (11 sides) with cavernous large and giant aneurysms treated during the past 8 years. Therapeutic strategies for each patient were selected according to the results of neurological and radiological examinations, and regional cerebral oxygen saturation A total of 6 high-flow bypasses were placed before ICA occlusions Three patients had STA-MCA bypasses before ICA occlusions, and two underwent endovascular ICA occlusion without bypass surgery. Favorable outcomes were obtained in all cases with respect to cranial nerve palsy, but one patient had insufficient ipsilateral cerebral blood flow postoperatively, and hemiparesis was revealed. An emergent STA-MCA bypass was performed in this case, which minimized the hemiparesis. Despite radiological evaluation during the BOT procedures, we had one false negative result. This indicates that the BOT requires increased sensitivity and specificity, and that a safety margin should be adopted when determining indications for bypass surgeries.
  • Daina Kashiwazaki, Satoshi Ushikoshi, Takeshi Asano, Satoshi Kuroda, Kiyohiro Houkin
    NEURORADIOLOGY 55 2 201 - 206 2013年02月 [査読有り][通常論文]
     
    Previous reports have suggested that endovascular parent artery occlusion is an effective and safe procedure for the treatment of vertebral artery dissection (VAD). However, the results of long-term outcomes are still unclear. This study reviewed the clinical and imaging outcomes of patients with VAD treated by endovascular internal trapping. A total of 73 patients were treated for VAD by endovascular internal trapping between March 1998 and March 2011. Patients were regularly followed up by magnetic resonance imaging, magnetic resonance angiography, and clinical examinations. Clinical outcomes were evaluated using the modified Rankin Scale. Forty-five patients had ruptured VADs, and 28 had unruptured VADs. Clinical follow-up of at least 6 months data was obtained for 61 patients (83.6 %). The follow-up period ranged from 6 to 145 months (mean +/- SD, 55.6 +/- 8.9 months). Two patients with ruptured VADs had recurrence (2.74 %). Cranial nerve paresis (CNP) was observed in six patients (8.21 %), spinal cord infarction in two patients (2.74 %), and a perforating artery ischemia was diagnosed in seven patients (9.59 %); all patients with CNP and five of the patients with partial Wallenberg syndrome experienced only temporary symptoms; two of the patients with partial Wallenberg syndrome had permanent neurological deficits. Despite their symptoms, most patients were in good general condition, as shown by their clinical scores. The results of this study have proven that endovascular internal trapping is a stable and durable treatment for closure of VADs. Recanalization is rather rare and occurred only in ruptured cases, both within 3 months after initial treatment without rupture. CNPs were observed in 8.21 %, perforating ischemia in 9.59 %, and spinal cord infarction in 2.74 %. The former two are temporary, while the last can be a factor that affects the modified Rankin Scale. Patients rated their quality of life as good, as corroborated by their posttreatment clinical score. Endovascular internal trapping for VAD is a therapy with a satisfactory long-term outcome.
  • Satoshi Kuroda, Daina Kashiwazaki, Tatsuya Ishikawa, Naoki Nakayama, Kiyohiro Houkin
    STROKE 44 2 516 - 518 2013年02月 [査読有り][通常論文]
     
    Background and Purpose-Clinical significance of silent microbleeds is unknown in moyamoya disease. This study was aimed to clarify the incidence, locations, and longitudinal course. Methods-This prospective cohort study included 78 nontreated patients with moyamoya disease. The incidence and locations of silent microbleeds were evaluated on T2*-weighted MRI. MR examinations were repeated every 6 or 12 months during a mean follow-up period of 43.1 months. Results-T2*-weighted MRI identified silent microbleeds in 17 (29.3%) of 58 adult patients with moyamoya disease, but in none of 20 pediatric patients. During follow-up periods, de novo silent microbleeds developed in 4 (6.9%) of 58 adult patients. Hemorrhagic stroke occurred in 4 patients (6.9%), all of who had silent microbleeds on initial examination. The presence of silent microbleeds was a significant predictor for subsequent hemorrhagic stroke in adult moyamoya disease (P<0.001). Conclusions-Careful and long-term follow-up of silent microbleeds would be essential to improve their outcome in adult patients with moyamoya disease. (Stroke. 2013;44:516-518.)
  • Shimbo Daisuke, Abumiya Takeo, Shichinohe Hideo, Nakayama Naoki, Kazumata Ken, Houkin Kiyohiro, Ishizuka Takanobu
    STROKE 44 2 2013年02月 [査読有り][通常論文]
  • Miyamoto Michiyuki, Kuroda Satoshi, Nakayama Naoki, Kazumata Ken, Houkin Kiyohiro
    STROKE 44 2 2013年02月 [査読有り][通常論文]
  • Ito Masaki, Houkin Kiyohiro, Niiya Yoshimasa, Uchino Haruto, Mabuchi Shoji, Nakayama Naoki, Kazumata Ken, Shichinohe Hideo, Sugiyama Taku, Ishii Nobuaki, Nomura Mikio
    STROKE 44 2 2013年02月 [査読有り][通常論文]
  • Hideo Shichinohe, Tomohiro Yamauchi, Hisayasu Saito, Kiyohiro Houkin, Satoshi Kuroda
    Acta Neurobiologiae Experimentalis 73 3 354 - 363 2013年 [査読有り][通常論文]
     
    This study was aimed to clarify if the bone marrow stromal cells (BMSCs) significantly improve functional outcome after lacunar stroke when stereotactically transplanted into the brain. Ouabain, a Na/K ATPase pump inhibitor, was stereotactically injected into the right striatum of Wistar rats. One week later, the superparamagnetic iron oxide (SPIO)-labeled rat BMSCs (n+AD0-7) or vehicle (n+AD0-8) were stereotactically transplanted into the left striatum. Using rotarod test, motor function was serially evaluated through the experiment. A 7.0-T MR apparatus was employed to serially monitor the migration of BMSCs in the host brain. Histological analysis was performed at 7 weeks after ouabain injection, i.e., 6 weeks after BMSC transplantation. Ouabain injection yielded the reproducible, focal lesion in the right striatum, causing continuous motor dysfunction throughout the experiment. BMSC transplantation significantly enhanced the recovery of motor function after ouabain injection. MR imaging demonstrated that the BMSCs aggressively migrated towards the lesion through the corpus callosum. Histological analysis supported the findings on MRI. The BMSCs significantly enhanced the neurogenesis in the subventricular zone (SVZ) on both sides. Some of them also expressed neuronal or astrocytic phenotypes in the neocortex, SVZ, corpus callosum, and peri-lesion area. These findings strongly suggest that the BMSCs may serve therapeutic impacts on lacunar stroke when stereotactically transplanted at clinically relevant timing. +ACY-copy+ADs. © 2013 by Polish Neuroscience Society - PTBUN, Nencki Institute of Experimental Biology.
  • Satoshi Kuroda, Kiyohiro Houkin
    Frontiers of Neurology and Neuroscience 32 62 - 68 2013年 [査読有り][通常論文]
     
    There is increasing evidence that the transplanted bone marrow stromal cells (BMSC) significantly promote functional recovery after central nervous system (CNS) damage in the animal models of various kinds of CNS disorders, including cerebral infarct. However, there remain several challenges before considering BMSC transplantation for patients with ischemic stroke. In this review, therefore, the authors discuss what should be clarified to establish cell transplantation therapy in the clinical setting and describe their scientific contributions in this matter. The BMSC have the ability to alter their gene expression profile and phenotype in response to the surrounding environment and to protect the neurons by producing certain neurotrophic factors. They also promote neurite extension and rebuild the neural circuits in the injured CNS. The BMSC can be expanded in vitro using the animal serum-free medium. Pharmacological modulation may accelerate the in vitro proliferation of the BMSC. Using in vivo optical imaging technique and MRI, the transplanted BMSC can noninvasively be tracked in the living animal for at least 8 weeks after transplantation. It is urgent to develop a clinical imaging technique to track the transplanted cells in the CNS and evaluate the therapeutic significance of BMSC transplantation in order to establish it as a definite therapeutic strategy in the clinical setting in the future.
  • Hisayasu Saito, Satoshi Kuroda, Kenji Hirata, Keiichi Magota, Tohru Shiga, Nagara Tamaki, Daisuke Yoshida, Satoshi Terae, Naoki Nakayama, Kiyohiro Houkin
    CEREBROVASCULAR DISEASES 35 4 370 - 377 2013年 [査読有り][通常論文]
     
    Background: Vulnerable and inflamed plaques in the carotid artery are at high risk of ischemic stroke, suggesting the importance of diagnostic modalities to detect them in patients with carotid stenosis with high sensitivity and specificity. Although many investigators have reported that magnetic resonance imaging (MRI) is a useful tool to predict the vulnerable components of carotid plaque, its validity is not established. On the other hand, F-18-fluorodeoxyglucose (FDG) positron emission tomography (PET) may be an alternative modality to directly identify the inflamed plaque in carotid artery stenosis. Therefore, this study aimed at evaluating the validity of MRI and FDG-PET to predict vulnerable and inflamed carotid plaque. Methods: This prospective study totally included 25 patients who underwent carotid endarterectomy (CEA) for carotid artery stenosis at our institute between January 2009 and January 2012. Prior to CEA, FDG-PET, black-blood T1-weighted imaging (BB-T1WI), and 3-dimensional time-of-flight (TOF) imaging were performed. The specimens were stained with hematoxylin-eosin to assess the different plaque components (lipid, hemorrhage, calcification, and fibrous tissue). In addition, they were stained with primary antibodies against CD68 (activated macrophages) and matrix metalloproteinase (MMP)-9. Results: High FDG uptake was detected in 13 (52.0%) of 25 patients. All of them had lipid-rich plaque. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) to identify the lipid-rich plaques were all 100% for FDG-PET. More importantly, all of the FDG-positive plaques had strong immunoreactivity against both CD68 and MMP-9. There was a significant correlation between the findings on FDG-PET and those on immunohistochemistry against CD68 and MMP-9 (p = 0.006 and 0.004, respectively). On the other hand, 16 (64.0%) of 25 patients had high signal intensity plaque on BB-T1WI. In 7 of these 16 patients, the lesions also showed high signal intensity on TOF imaging. All of them had a large intraplaque hemorrhage. The sensitivity, specificity, PPV, and NPV to identify a large intraplaque hemorrhage were 70, 100, 100, and 83%, respectively, for MRI. Conclusions: These findings suggest that FDG-PET and MRI are complementary to predict high-risk carotid plaque, such as lipid-rich or hemorrhagic plaque. FDG-PET can accurately predict the lipid-rich and inflamed plaque. MRI is valuable to identify unstable plaque with a large intraplaque hemorrhage. The combination of these two modalities may play an important role in predicting carotid plaque at high risk of ischemic stroke. Copyright (C) 2013 S. Karger AG, Basel
  • Toru Sasamori, Kazutoshi Hida, Takeshi Asano, Toshiya Osanai, Syunsuke Yano, Takeshi Aoyama, Yoshinobu Iwasaki, Kiyohiro Houkin
    Neurologia medico-chirurgica 53 2 103 - 6 2013年 [査読有り][通常論文]
     
    A 60-year-old woman presented with a spinal dural arteriovenous fistula (SDAVF) located in the lower cervical region, which had been asymptomatic for 56 months after the initial detection. She underwent embolization of the SDAVF when she became symptomatic, but her neurological recovery was only partial. Cervical SDAVF is rare but sometimes discovered in an asymptomatic state. The natural history and validity of preventive treatment for asymptomatic SDAVF have not been fully established. Her unfavorable outcome demonstrates the potential advantages of adequate treatment at an early stage even for asymptomatic SDAVF.
  • Hiroshi Kanno, Jun-ichi Kuratsu, Ryo Nishikawa, Kazuhiko Mishima, Atushi Natsume, Toshihiko Wakabayashi, Kiyohiro Houkin, Shunsuke Terasaka, Taro Shuin
    ACTA NEUROCHIRURGICA 155 1 1 - 7 2013年01月 [査読有り][通常論文]
     
    Central nervous system (CNS) hemangioblastoma (HB) is one of the most common manifestations in von Hippel-Lindau disease (VHL), but large-scale studies on clinical features of CNS HB in VHL are scarce. On the basis of the results of a questionnaire, we collected data of VHL patients with CNS HB. The total number of CNS HBs in 111 VHL patients (male 59, female 52) was 264 with the following distributions: cerebellar, 65.4 %; brainstem, 9.9 %; spinal cord, 23.9 %; and pituitary, 1. 1 %. The follow-up period was 0.6 to 39.2 years, with the mean 12.5 years. Patients bearing brainstem or spinal cord HB also had another HB significantly more frequently than those bearing cerebellar HBs (P < 0.05). The mean onset age of CNS HB was 29.1 years, and that of patients bearing a single HB (mean 34.4 years) was significantly greater than that of multiple HBs (mean 25.7 years). Patients with multiple HBs under 40 years are more dominant than those with a single HB. The distribution rate of brainstem HB is significantly smaller in patients below 30 years than patients above 29 years. Although ECOG PS score increased along with number of operations, the onset age decreased with increasing number of operations. The mean ECOG PS score of patients below 20 years is significantly smaller than patients above 19 years. When the onset age of CNS HB is under 40 years, and CNS HB is located at the brainstem or spinal cord HB, the probability of multiple occurrence can be predicted. Since patients with an onset age under 20 years old preserve a high performance status, early detection of CNS HB would be important. In addition, since a multiple operations aggravate performance status, number of operations should be reduced.
  • Michiyuki Miyamoto, Satoshi Kuroda, Songji Zhao, Keiichi Magota, Hideo Shichinohe, Kiyohiro Houkin, Yuji Kuge, Nagara Tamaki
    Journal of Nuclear Medicine 54 1 145 - 150 2013年01月 [査読有り][通常論文]
     
    This study aimed to assess whether 18F-FDG PET could serially monitor the beneficial effects of bone marrow stromal cells (BMSC) on cerebral glucose metabolism when transplanted into the infarct brain of rats. Methods: The BMSC from green fluorescent protein transgenic rats or vehicle was stereotactically transplanted into the ipsilateral striatum at 7 d after permanent middle cerebral artery occlusion of rats. Local glucose metabolism was semiquantitatively measured at 6 and 35 d after ischemia using 18F-FDG PET. Motor function was serially evaluated throughout the experiments. At 35 d after ischemia, immunohistochemistry was performed to evaluate the phenotype of BMSC and their effects on the expression of brain-type glucose transporters. Results: BMSC transplantation not only enhanced functional recovery but also promoted the recovery of glucose utilization in the periinfarct area when stereotactically transplanted at 1 wk after ischemia. The engrafted cells were widely distributed, and most expressed a neuron-specific protein, NeuN. BMSC transplantation also prevented the pathologic upregulation of glucose transporters in the periinfarct neocortex. Conclusion: The present findings strongly suggest that the BMSC may enhance functional recovery by promoting the recovery of local glucose metabolism in the periinfarct area when directly transplanted into the infarct brain at clinically relevant timing. The BMSC also inhibit the pathologic upregulation of brain-isoform glucose transporters type 1 and 3. 18F-FDG PET may be a valuable modality to scientifically prove the beneficial effects of BMSC transplantation on the host brain in clinical situations. COPYRIGHT © 2013 by the Society of Nuclear Medicine and Molecular Imaging, Inc.
  • 齊藤正樹, 板本孝治, 中村正弘, 三國信啓, 中川原譲二, 三浦哲嗣, 寳金清博, 深津恵美
    北海道医報 1134 2013年
  • 飛騨一利, 長内俊也, 青山剛, 矢野俊介, 関敏孝, 岩崎喜信, 寳金清博
    脳神経外科ジャーナル 22 Supplement 2013年
  • 七戸秀夫, 寳金清博
    血液事業 36 2 2013年
  • 寳金清博
    脳循環代謝 25 1 2013年
  • 七戸秀夫, 山内朋裕, 斎藤久泰, 寳金清博, 黒田敏
    脳循環代謝 25 1 2013年
  • 七戸秀夫, 寳金清博
    神経治療学 30 5 2013年
  • 茂木洋晃, 寺坂俊介, 小林浩之, 本間明宏, 加納里志, 畠山博充, 水町貴諭, 福田諭, 寳金清博
    日本脳腫瘍の外科学会プログラム・抄録集 18th 2013年
  • 伊師雪友, 寺坂俊介, 山崎和義, 茂木洋晃, 小林浩之, 寳金清博, 井口晶裕, 長祐子, 大島淳二郎, 佐藤智信
    日本脳腫瘍の外科学会プログラム・抄録集 18th 2013年
  • 七戸秀夫, 寳金清博
    週刊日本医事新報 4663 2013年
  • 寺坂俊介, 小林浩之, 茂木洋晃, 金子貞洋, 遠藤将吾, 寳金清博, 石津桂, 白土博樹
    日本小児血液・がん学会雑誌 50 2 2013年
  • 遠藤将吾, 寺坂俊介, 金子貞洋, 小林浩之, 浅岡克行, 鴨嶋雄大, 山口秀, 茂木洋晃, 畑中佳奈子, 寳金清博
    小児の脳神経 38 1 2013年
  • Haruto Uchino, Toshitaka Nakamura, Satoshi Kuroda, Kiyohiro Houkin, Jun-ichi Murata, Hisatoshi Saito
    WORLD NEUROSURGERY 78 6 651 - 657 2012年12月 [査読有り][通常論文]
     
    BACKGROUND: Carotid endarterectomy (CEA) is a useful procedure to prevent subsequent ischemic stroke in patients with severe stenosis of internal carotid artery. However, lowering of morbidity is still essential to keep its clinical significance. This study aimed to evaluate the validity of dual monitoring using transcranial motor evoked potential (MEP) and near-infrared spectroscopy (NIRS) during CEA. METHODS: Transcranial MEP and NIRS monitoring were conducted in 20 consecutive CEAs. MEP was recorded in the contralateral extremities. Regional cerebral saturation of oxygen (rSO(2)) was continuously measured in the ipsilateral forehead. The changes of MEP amplitudes and rSO(2) during cross-clamping of carotid artery were compared in each case. RESULTS: The amplitudes of MEP significantly decreased when rSO(2) reduced to more than 20% during carotid clamping. There was a significant correlation between the changes of MEP amplitude and rSO(2) during carotid clamping in a quadratic manner (P < .001, r = 0.821). However, NIRS could not detect critical cerebral ischemia in 1 patient with cerebral infarction in the ipsilateral frontal lobe. On the other hand, MEP could not identify it in 1 patient with severe motor deficit. No perioperative complication occurred. CONCLUSIONS: These findings strongly suggest that both MEP and NIRS can detect critical cerebral ischemia during CEA in most patients. Dual MEP and NIRS monitoring may further increase the sensitivity to identify it, being valuable to prevent perioperative complications due to cerebral ischemia during CEA.
  • Makoto Saito, Yoshimitsu Takahashi, Yayoi Yoshimura, Ayako Shima, Akio Morita, Kiyohiro Houkin, Takeo Nakayama, Kazuhiko Nozaki
    NEUROLOGIA MEDICO-CHIRURGICA 52 12 873 - 877 2012年12月 [査読有り][通常論文]
     
    Communication between patients with cerebral aneurysms and consulting neurosurgeons remains unstudied in Japan. The present clinical study surveyed patients with unruptured cerebral aneurysms and their neurosurgeons after explanation of the disease and its treatment options and expected outcomes in clinic visits using a one-page written questionnaire about treatment options and decisions given to patients and their neurosurgeons. The numbers of participating patients and neurosurgeons were 42 and 9, respectively, and 42 paired patient-neurosurgeon responses were obtained. Agreement was quite low (kappa = 0.17-0.31 for 6-point Likert scale and kappa = 0.44-0.67 for 2 category scale) regarding the "best" treatment for each patient as agreed on by the patient and neurosurgeon. Agreement in the understanding of treatment options and general application was unexpectedly low (kappa = 0.12 and 0.01 for 6-point Likert scale and kappa = not applicable and -0.03, respectively, for 2 category scale). Agreement tended to be higher between experienced neurosurgeons and patients than non-experienced neurosurgeons and patients. Patients estimated much higher risks of stroke or death after surgical intervention (p < 0.001) or no intervention (p = 0.006) compared with the estimates offered by their neurosurgeons.
  • Masahito Kawabori, Tetsuyuki Yoshimoto, Masaki Ito, Shin Fujimoto, Taisei Mikami, Mutsuko Muraki, Sadao Kaneko, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 52 12 885 - 891 2012年12月 [査読有り][通常論文]
     
    Spontaneous echo contrast (SEC) consists of numerous microechoes swirling in the cardiovascular lumen and is usually seen during blood stasis in dysfunctional left atrium. However, SEC and consecutive local thrombus formation at the carotid artery early after carotid endarterectomy (CEA) have not been reported. This study retrospectively investigated the clinical importance and therapeutic strategy of postoperative SEC and thrombus formation in 113 consecutive patients who underwent CEA between 2001 and 2009. Ultrasonography was routinely performed preoperatively, intraoperatively, and 1 day and 1 week after the operation. If SEC and/or thrombus was detected at any time after the operation, follow-up ultrasonography was performed at short intervals, once a week for inpatients and once every 1-2 months for outpatients. Eight of the 113 patients (7%) had SEC after the operation from Day 1 to 12 (mean 7.2 days), and 6 of these 8 patients developed local de novo thrombus formation at the site of SEC from Day 6 to 33 (mean 14.7 days). The maximum luminal narrowing by the thrombi were 26-62% (mean 37%). After administering anticoagulant therapy, all thrombi disappeared from Day 13 to 190 (mean 57 days) from CEA. SEC seen after CEA is highly associated with consecutive local thrombus formation. Postoperative geometric blood stasis with the absence of intima may be the causative factor for its development.
  • Kota Kurisu, Masahito Kawabori, Yoshimasa Niiya, Yuzuru Ohta, Naoki Nakayama, Satoshi Kuroda, Shoji Mabuchi, Kiyohiro Houkin
    CLINICAL NEUROLOGY AND NEUROSURGERY 114 9 1274 - 1276 2012年11月 [査読有り][通常論文]
  • Kota Kurisu, Masahito Kawabori, Yoshimasa Niiya, Yuzuru Ohta, Shoji Mabuchi, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 52 11 822 - 825 2012年11月 [査読有り][通常論文]
     
    An 86-year-old female presented with rare bilateral chronic subdural hematomas (CSHs) of the posterior fossae which were successfully treated by surgical intervention. She had experienced mild head trauma one month before admission. She was transferred to our hospital because of consciousness disturbance and tetraparesis. Magnetic resonance (MR) imaging showed simultaneous occurrence of supratentorial and infratentorial CSHs. We tried to evacuate the CSHs of the bilateral posterior fossae because brainstem compression was markedly severe. Through bilateral burr-hole trepanations, chocolate-colored fluid, not containing clotted components, gushed out under great pressure. Postoperative course was uneventful. MR imaging revealed that the CSHs of the posterior fossae had completely disappeared and brainstem compression had also improved. The patient's neurological deficits were immediately improved after the operation. The patient was discharged one month after the operation for further rehabilitation. Trepanation and evacuation of the hematoma through the posterior fossa might be one of the therapeutic options for posterior fossa CSH, which is similar to supratentorial CSH. However, we considered that the emergency of this rare entity and the method of anesthesia were quite different from supratentorial CSH.
  • 新保 大輔, 鐙谷 武雄, 七戸 秀夫, 中山 若樹, 数又 研, 宝金 清博, 石塚 隆伸
    脳循環代謝 24 1 178 - 178 (一社)日本脳循環代謝学会 2012年11月 [査読無し][通常論文]
  • Haruto Uchino, Satoshi Kuroda, Kenji Hirata, Tohru Shiga, Kiyohiro Houkin, Nagara Tamaki
    STROKE 43 10 2610 - 2616 2012年10月 [査読有り][通常論文]
     
    Background and Purpose-Clinical features and pathophysiology of postoperative hyperperfusion in moyamoya disease are still unclear. This study was aimed to clarify the incidence and time course of postoperative hyperperfusion and to determine the independent predictors of postoperative hyperperfusion in moyamoya disease. Methods-This prospective study included 41 patients who underwent surgical revascularization for moyamoya disease. Using O-15-gas positron emission tomography, hemodynamic and metabolic parameters were quantified before surgery. Using single photon emission computed tomography, cerebral blood flow was serially measured just after surgery and on 2 and 7 days postsurgery. A multivariate logistic regression analysis was conducted to test the effect of multiple variables on postoperative hyperperfusion. Results-Postoperative hyperperfusion was observed in 29 (50.0%) of 58 operated hemispheres. The incidence of both radiological and symptomatic hyperperfusion was significantly higher in adult patients than in pediatric ones (P=0.026 and P=0.0037, respectively). Hyperperfusion just after surgery more often led to subsequent neurological deficits (P=0.033). A multivariate analysis revealed that preoperative cerebral blood volume increase was an independent predictor of both radiological and symptomatic hyperperfusion after surgery in adult moyamoya disease (OR, 6.6 and 12.3, respectively). Conclusions-Postoperative hyperperfusion after surgical revascularization is not rare in moyamoya disease. Adult patients with a cerebral blood volume increase may be at high risk for radiological and symptomatic hyperperfusion after surgery. Careful perioperative management would reduce surgical complications and improve long-term outcome in moyamoya disease. (Stroke. 2012; 43:2610-2616.)
  • Masaki Ito, Satoshi Kuroda, Taku Sugiyama, Katsuhiko Maruichi, Masahito Kawabori, Naoki Nakayama, Kiyohiro Houkin, Yoshinobu Iwasaki
    NEUROPATHOLOGY 32 5 522 - 533 2012年10月 [査読有り][通常論文]
     
    This study was aimed to assess whether bone marrow stromal cells (BMSC) could ameliorate brain damage when transplanted into the brain of stroke-prone spontaneously hypertensive rats (SHR-SP). The BMSC or vehicle was stereotactically engrafted into the striatum of male SHR-SP at 8 weeks of age. Daily loading with 0.5% NaCl-containing water was started from 9 weeks. MRIs and histological analysis were performed at 11 and 12 weeks, respectively. Wistar-Kyoto rats were employed as the control. As a result, T2-weighted images demonstrated neither cerebral infarct nor intracerebral hemorrhage, but identified abnormal dilatation of the lateral ventricles in SHR-SP. HE staining demonstrated selective neuronal injury in their neocortices. Double fluorescence immunohistochemistry revealed that they had a decreased density of the collagen IV-positive microvessels and a decreased number of the microvessels with normal integrity between basement membrane and astrocyte end-feet. BMSC transplantation significantly ameliorated the ventricular dilatation and the breakdown of neurovascular integrity. These findings strongly suggest that long-lasting hypertension may primarily damage neurovascular integrity and neurons, leading to tissue atrophy and ventricular dilatation prior to the occurrence of cerebral stroke. The BMSC may ameliorate these damaging processes when directly transplanted into the brain, opening the possibility of prophylactic medicine to prevent microvascular and parenchymal-damaging processes in hypertensive patients at higher risk for cerebral stroke.
  • Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Nobuaki Ishii, Masaki Ito, Daisuke Shimbo, Kanako Kubota, Kiyohiro Houkin
    BRAIN TUMOR PATHOLOGY 29 4 240 - 244 2012年10月 [査読有り][通常論文]
     
    Rhabdoid meningioma (RM) is a rare aggressive phenotype and is classified as a grade III neoplasm by the World Health Organization. A 29-year-old woman initiated treatment with clomiphene citrate for infertility. Two weeks later, she presented with acute headache and nausea. Brain computed tomography and magnetic resonance imaging demonstrated a tumor with hematoma in the left frontoparietal region. Surgical resection was performed, and the tumor was subtotally removed. The tumor was diagnosed as a rhabdoid meningioma (RM). Despite radiation and chemotherapy, she experienced regrowth and dissemination to the spinal cord. She died 11 months after onset of symptoms. Spontaneous hemorrhage is an unusual presentation of RM. In our case, infertility treatment may have triggered progression and bleeding because of an imbalance of sex hormones.
  • Masaki Ito, Kiyohiro Houkin, Hisayasu Saito, Daisuke Shimbo, Hiroaki Motegi, Masahito Kawabori, Michiyuki Miyamoto, Tomohiro Yamauchi
    Neurological Surgery 40 10 923 - 945 2012年10月 [査読有り][通常論文]
     
    Cerebrospinal fluid (CSF) shunts are commonly employed to treat patients with hydrocephalus. A large number of papers have been published focusing on complications and failures of CSF shunts. However, there appears to be a paucity of knowledge comprehensively covering both common complications and rare ones. In this systematic review, we surveyed articles about surgical complications of CSF shunts as comprehensively as possible. Quantitative analysis was performed to determine the frequency of well-known complications, mortality and revision rates of CSF shunts. Furthermore, rare complications of CSF shunts have also been reviewed.
  • KASHIWAZAKI Daina, TERASAKA Shunsuke, KAMOSHIMA Yuuta, KUBOTA Kanako, ASANO Takeshi, HOUKIN Kiyohiro
    Neurologia medico-chirurgica = 神経外科 52 10 745 - 747 一般社団法人 日本脳神経外科学会 2012年10月 [査読有り][通常論文]
     
    A 46-year-old man with factor VIII deficiency presented with a rare case of hemophilic pseudotumor in the temporal bone manifesting as severe conductive hearing loss and external ear bleeding. The pseudotumor expanded and destroyed the temporal bone and skin of the external ear over the course of 8 years. The pseudotumor was surgically excised, and the patient's symptoms improved. Histological examination of a specimen collected from inside the pseudotumor demonstrated blood products in various stages of evolution and showed that the outer membrane consisted of a collagen layer. Hemophilic pseudotumors are rare complications occurring in 1-2% of patients with mild or severe hemophilia. Pseudotumors are chronic, slowly expanding, encapsulated cystic masses, and most are located in the long bones and pelvis. The present case suggests that cranial pseudotumor should be considered in the differential diagnosis of cranial lesion in a patient with hemophilia.
  • Yuuta Kamoshima, Shunsuke Terasaka, Hiroyuki Kobayashi, Sadahiro Kaneko, Kanako Kubota, Shinya Tanaka, Kiyohiro Houkin
    CLINICAL NEUROLOGY AND NEUROSURGERY 114 7 1077 - 1080 2012年09月 [査読有り][通常論文]
  • SASAMORI Toru, HIDA Kazutoshi, YANO Shunsuke, AOYAMA Takeshi, ASANO Takeshi, KUBOTA Kanako, ITO Manabu, ABUMI Kuniyoshi, IWASAKI Yoshinobu, SAITO Hisatoshi, HOUKIN Kiyohiro
    Neurologia medico-chirurgica = 神経外科 52 9 670 - 674 一般社団法人 日本脳神経外科学会 2012年09月 [査読有り][通常論文]
     
    A 25-year-old man presented with malignant transformation to malignant peripheral nerve sheath tumor (MPNST) in the cervical spine associated with neurofibromatosis type 1. He presented with a 3-week history of rapidly increasing weakness and numbness in all four extremities. Magnetic resonance (MR) imaging of the cervical spine demonstrated a dumbbell-shaped tumor, which compressed the spinal cord at the C2-3 level. The tumor was excised, mainly within the spinal canal to decompress the spinal cord. The histological diagnosis was benign neurofibroma. Three months after surgery, he rapidly developed progressive tetraparesis and MR imaging revealed marked regrowth of an extradural mass into the spinal canal. At reoperation, the regrown mass in the spinal canal was totally excised. The histological diagnosis revealed MPNST. He underwent radiation therapy, with a total dose of 32 Gy, for approximately 3 weeks after the second surgery, but MR imaging showed tumor regrowth within the spinal canal, and his condition deteriorated. The decision was made to remove the tumor radically, including the involved facet and extradural lesion. Posterior fusion using a pedicle screw was performed one month later. He manifested no additional neurological deficits. He has been free of relapse for 46 months. Radical resection remains the most effective treatment for MPNST, although complete removal with a clear tumor margin is often impossible in practice.
  • Toshiya Osanai, Kazutoshi Hida, Takeshi Asano, Takeshi Aoyama, Kiyohiro Houkin
    No shinkei geka. Neurological surgery 40 8 695 - 703 2012年08月 [査読有り][通常論文]
     
    PURPOSE: Multi-detector-row CT angiography (MDCTA) is useful for diagnosis of spinal arteriovenous (AV) shunt. So we reported the role and pitfall of MDCTA based on our experience. SUBJECTS: From January 2007 to September 2011, 57 cases used MDCTA in our institution. We investigated 22 case of spinal dural AVF and 5 cases of epidural AVF. METHODS: All cases were performed by Toshiba Aquilion 64. FOV was the whole spine, curved planar reconstruction along the spinal canal, sagittal and axial view obtained by multi-planar reconstruction. RESULTS: MDCTA detected dilated the perimedullary vein in all cases and identified the level of segmental artery matching the result of DSA in 16 cases of spinal dural AVF (73%) and 1 cases of epidural AVF (20%). CONCLUSION: MDCTA is useful for screening, and identifying the shunt point, but care needs to be taken concerning the particular pitfall of MDCTA.
  • Hisayasu Saito, Naoki Nakayama, Shugo Takikawa, Satoshi Ushikoshi, Daisuke Shinbo, Satoshi Kuroda, Kiyohiro Houkin
    Neurological Surgery 40 8 717 - 722 2012年08月 [査読有り][通常論文]
     
    Isolated abducens nerve palsies associated with the rupture of intracranial aneurysms have rarely been reported. We report two cases of isolated bilateral abducens nerve palsies occurring after subarachnoid hemorrhage due to the rupture of an intracranial aneurysm. Case 1 : A 49-year-old woman had bilateral abducens nerve palsies following subarachnoid hemorrhage due to the rupture of the left vertebral artery-posterior inferior cerebellar artery aneurysm. Case 2: A 55-year-old man had bilateral abducens nerve palsies following subarachnoid hemorrhage due to dissecting aneurysm of the right vertebral artery. Case 1 and 2 were treated with surgical clipping of the aneurysm and internal occlusion of the parent artery. In both cases, bilateral abducens nerve palsies achieved almost full recovery several months after treatment. It is speculated that the main causes of palsies are compression and stretching of the bilateral abducens nerves by a thick clot in the prepontine cistern. Although most of the abducens palsies may be reversible and have good prognosis, it is important that they are kept in mind as isolated symptoms of subarachnoid hemorrhage.
  • Masahito Kawabori, Ken Kazumata, Kosuke Ohnishi, Taku Sugiyama, Masaki Itoh, Naoki Nakayama, Kiyohiro Houkin
    Neurological Surgery 40 8 731 - 740 2012年08月 [査読有り][通常論文]
     
    Although a large number of patients with unruptured middle cerebral artery (MCA) aneurysms (AN) have been treated by surgical clipping in Japan, there has yet been no comprehensive study investigating the surgical risks based on a quantitative evaluation of the extensive existing body of patient records. This systematic review was conducted to determine morbidity of the procedure by performing a meta-analysis of the literature. The authors used a PubMed and J-stage search from 2000 to 2011 for studies containing the surgical clipping of the unruptured MCA AN. There were 21 articles, containing a total 1.323 cases of unruptured AN with morbidity specifically located in the MCA. 54 cases indicated significant neurological deficits for a morbidity rate of 4.1% (95% CI 3.0-5.1). A limited number of studies disclosed an incremental increase in morbidity with the size of the aneurysm. Smaller MCA AN (7±3 mm) presented a lower morbidity of 1.48%. whereas giant MCA AN (> 25 mm) corresponded with a higher morbidity of 27.8%. Factors consistently associated with high morbidity included incorporated MCA branches, plaque at the neck of the AN, an unclippable configuration, and M1 superior wall AN. Complex aneurysms required a wide array of intracranial bypass procedures, yielding morbidity of 23.4% (95% CI 20.9-25.9). This is the first systematic review and quantitative meta-analysis of the surgical complications related to unruptured MCA AN.
  • Kota Kurisu, Masahito Kawabori, Yoshimasa Niiya, Yuzuru Ohta, Shoji Mabuchi, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 52 8 587 - 590 2012年08月 [査読有り][通常論文]
     
    A 68-year-old man presented with severe conscious disturbance caused by pituitary apoplexy resulting in massive intracerebral hemorrhage (ICH). He had been periodically followed up for asymptomatic pituitary adenoma at another hospital for 8 years. Neuroimaging examination revealed pituitary apoplexy and massive ICH located in the left frontal lobe, and the ICH was directly connected to the intratumoral hemorrhage. The diagnosis was massive ICH from pituitary apoplexy. The patient underwent emergent evacuation of hematoma and removal of the pituitary adenoma via bi-frontal craniotomy. Postoperatively, he continued to exhibit deep consciousness disturbance and died 1 month after the operation. Pituitary apoplexy is usually characterized by intra-tumoral hemorrhage. The treatment strategy for asymptomatic pituitary adenoma is still controversial. This case shows that we should always consider the risk of pituitary apoplexy manifesting as ICH which may cause a fatal outcome.
  • Motegi H, Kamoshima Y, Terasaka S, Kobayashi H, Houkin K
    [Hokkaido igaku zasshi] The Hokkaido journal of medical science 87 4-5 147 - 151 4-5 2012年08月 [査読有り][通常論文]
     
    BACKGROUND AND AIMS: Glioblastoma is one of the most malignant brain tumors, causing death within two years despite maximal tumor resection and concurrent radio-chemotherapy. Tumor stem cells are thought to be closely related to tumor progression and recurrence and are attractive therapeutic targets. It is common to culture cell lines in serum-free medium with growth factors to stimulate spheres of enriched tumor stem cells. To avoid spontaneous differentiation and cell death in the sphere environment, Pollard et al. formulated an adherent culture method using laminin-coated plates. We here evaluated collagen-1-coated plates, which are superior to laminin-coated plates in handling and cost, as an alternative adherent culture method of CD133 expressing glioblastoma cells. MATERIALS AND METHODS: We cultured the human glioblastoma cell line, U87MG, under serum contained medium (SCM) or serum free medium with EGF, FGF2, LIF, B27 and N2 supplements (SFM) in non-coated, laminin or collagen-1-coated plates. The growth morphology in various cultures was evaluated, and the number of living cells in each plate on day 5 after cell seeding was calculated. The RNA expression level of CD133 as a stem cell marker in each plate was examined. Semi-quantitative measurement of CD133 positive cells by immunocytochemistry was performed. RESULTS: In collagen-1-coated plates with SFM, cell lines were cultured in an adherent monolayer. Cell proliferation was statistically encouraged in collagen-1-coated plates. Both laminin-coated plates and collagen-1-coated plates with SFM enhanced the RNA expression of CD133 compared to non-coated plates with SCM. Immunocytochemistry showed a statistically significant increase of CD133 positive cells in collagen-1-coated plates with SFM. CONCLUSION: Collagen-1-coated plates with SFM was used for cell morphology and cell proliferation of CD133 expression in the U87MG malignant glioma cell line.
  • 穂刈 正昭, 中山 若樹, 黒田 敏, 長内 俊也, 濱内 祝嗣, 遠藤 将吾, 宝金 清博
    脳神経外科速報 22 8 938 - 942 (株)メディカ出版 2012年08月 [査読無し][通常論文]
     
    46歳女。前交通動脈瘤破裂に対してクリッピング術を行い、術後の脳血管撮影でcomplete clippingを確認していたが、19年後に動脈瘤の再発を認めた。初回治療時には右A1-2 bifurcation typeの動脈瘤に対してpterional approachで前交通動脈(AcomA)に平行に過不足ないクリッピングが行われていたが、AcomA側に及んでいた壁の薄い部分(クリップブレード上側脇)から動脈瘤が再発し、前および上向きに伸展したと考えられた。両側前開頭interhemisphaericアプローチでクリッピング術を行い、以前のクリップを外すことなく母動脈に対して直交するかたちで余すことなくクリッピングできた。
  • Taku Sugiyama, Naoki Nakayama, Shunsuke Terasaka, Satoshi Kuroda, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 52 7 506 - 509 2012年07月 [査読有り][通常論文]
     
    A 28-year-old woman presented with an unusual case of giant thrombosed varix with calcified walls that had mass effects secondary to a pial single-channel arteriovenous fistula (AVF) associated with hereditary hemorrhagic telangiectasia (HHT). She consulted our hospital for chronic headache. She had been diagnosed with HHT based on genetic testing when her 3-year-old son presented with subarachnoid hemorrhage due to spinal AVF. Imaging studies revealed pial single-channel AVF with multiple varices. The varices in the right frontal lobe were over 6 cm in diameter and had laminar thromboses and calcified walls. Because of the mass effect, direct surgical flow disconnection was performed followed by removal of the varices using an internal decompression technique. Postoperatively, the patient was discharged with no neurological symptoms and no longer suffered chronic headache. Intracerebral varices are occasionally associated with high-flow AVF, and usually treated by interrupting the feeding arteries leaving the varices intact. This extremely rare case of intracerebral giant thrombosed varices with calcified wall and mass effect indicates that surgical removal of varices should be considered.
  • Toshiya Osanai, Takeshi Asano, Shunsuke Terasaka, Kiyohiro Houkin
    No shinkei geka. Neurological surgery 40 7 599 - 606 2012年07月 [査読有り][通常論文]
     
    Preoperative embolization for hypervascular tumors is typically performed using particle agents such as polyvinyl alcohol, gelfoam powder, and fibrin glue. Furthermore, n-butyl-2-cyanoacrylate (NBCA) is used in patients with cerebral arteriovenous malformation (AVM) or an AV shunt. However, few reports have described the efficacy of NBCA in treating hypervascular tumors. Here, we report cases of hypervascular tumors in which preoperative embolization was performed using NBCA. We also discuss the difference between the efficacy of a liquid agent and a particle agent for hypervascular tumor embolization. We analyzed 10 cases encountered at our institution since 2004 in which preoperative embolization was performed using NBCA. In all cases, NBCA was injected through the tumor-feeding artery. In eight of these cases, preoperative embolization decreased intraoperative bleeding and markedly reduced the tumor stain. In the remaining two cases, complications occurred but without any permanent sequel. Thus, NBCA is useful for preoperative embolization.
  • Kengo Suzuki, Masahiko Wanibuchi, Yukinori Akiyama, Jun Ikeda, Yoshihiro Minamida, Tadashi Hasegawa, Kiyohiro Houkin, Nobuhiro Mikuni
    Neurological Surgery 40 7 617 - 621 2012年07月 [査読有り][通常論文]
     
    We described a case of primary clear cell carcinoma (CCC) of the paranasal cavity and skull base. A 59-year-old female experienced chronic nasal obstruction and double vision. Computed tomography (CT) scan revealed a mass lesion of the paranasal cavity, reaching the skull base and intracranial zone. We performed a biopsy by endonasal endoscopic surgery and pathological examinations revealed the tumor was a clear cell carcinoma. No primary tumor was identified on evaluation of the whole body by CT scan and FDG-PET ( 18fluorodeoxy glucose positron emission tomography) scan, so we diagnosed a primary CCC of the paranasal cavity. We decided to use stereotactic radiation therapy. Neurologically, her diplopia was improved. The patient is well at 2 years of follow-up with no evidence of local enlargement. There are some reports about the metastatic sinonasal CCC, but there are few reports about primary nasal and sinonasal CCC. This is the fourth case report of primary nasal and sinonasal CCC.
  • Hokari M, Nagauchi T, Nakayama N, Hamauchi S, Ito Y, Shimoda Y, Houkin K
    No shinkei geka. Neurological surgery 40 651 - 655 7 2012年07月 [査読有り][通常論文]
  • Masaaki Hokari, Toshiya Osanai, Naoaki Nakayama, S Hamauchi, Yasuhiro Ito, Yusuke Shimoda, Kiyohiro Houkin
    No shinkei geka. Neurological surgery 40 7 651 - 5 2012年07月 [査読有り][通常論文]
  • 中山 若樹, 宝金 清博
    医学と薬学 68 1 30 - 40 (株)自然科学社 2012年07月 [査読無し][通常論文]
  • Takeshi Asano, Satoshi Kuroda, Kiyohiro Houkin, Daisuke Yoshida, Kazutoshi Cho, Hideaki Shiraishi, Shinji Saito
    Neurological Surgery 40 6 519 - 525 2012年06月10日 [査読有り][通常論文]
     
    We report a case of vein of Galen aneurysmal malformation (VGAM) with a newly developed dural arteriovenous fistula (AVF) subsequent to successful embolization. A male neonate diagnosed as VGAM with prenatal ultrasonography and MRI presented severe cardiac and respiratory failure soon after birth. Five sessions of transarterial embolization using NBCA were performed during the first 6 months of his life. The shunt flow was effectively reduced and heart failure was resolved after the treatment. Follow-up angiography performed 2.5 years after the last embolization revealed complete obliteration of VGAM and newly developed small dural AVF on the wall of the thrombosed falcorial sinus. We believe that the dural AVF in this case was caused by local venous hypertension or induction of angiogenic factor during the thrombosing process of VGAM.
  • Sugiyama T, Houkin K, Ito M, Hokari M, Nakayama N, Kazumata K, Kuroda S
    No shinkei geka. Neurological surgery 40 6 555 - 565 6 2012年06月 [査読有り][通常論文]
     
    In Japan, a large number of surgical treatments for unruptured intracranial aneurysms have been performed. On the other hands, it is known that surgical treatment for unruptured intracranial aneurysms is the most frequent source of lawsuits in the neurosurgical field. Neurosurgeons have the duty to disclose all risks and consequences of a proposed surgical procedure to each patient. Moreover, precise knowledge of surgical risks would be helpful in preventing complications from occurring. However, it is almost impossible that a single surgeon or an institute have experiences with all possible surgical complications because there is limitation of the number of surgery. In this review series, we attempted an exhaustive bibliographic survey of the possible surgical complications including exceptional ones, and then, determined the frequency of each complications as far as possible quantitatively. In this paper, we focused on the complications of surgical treatment for anterior cerebral artery aneurysms.
  • Masahito Kawabori, Satoshi Kuroda, Taku Sugiyama, Masaki Ito, Hideo Shichinohe, Kiyohiro Houkin, Yuji Kuge, Nagara Tamaki
    NEUROPATHOLOGY 32 3 217 - 226 2012年06月 [査読有り][通常論文]
     
    Recent studies have indicated that bone marrow stromal cells (BMSC) may improve neurological function when transplanted into an animal model of CNS disorders, including cerebral infarct. However, there are few studies that evaluate the therapeutic benefits of intracerebral and intravenous BMSC transplantation for cerebral infarct. This study was aimed to clarify the favorable route of cell delivery for cerebral infarct in rats. The rats were subjected to permanent middle cerebral artery occlusion. The BMSC were labeled with near infrared (NIR)-emitting quantum dots and were transplanted stereotactically (1 x 10(6) cells) or intravenously (3 x 10(6) cells) at 7 days after the insult. Using in vivo NIR fluorescence imaging technique, the behaviors of BMSC were serially visualized during 4 weeks after transplantation. Motor function was also assessed. Immunohistochemistry was performed to evaluate the fate of the engrafted BMSC. Intracerebral, but not intravenous, transplantation of BMSC significantly enhanced functional recovery. In vivo NIR fluorescence imaging could clearly visualize their migration toward the cerebral infarct during 4 weeks after transplantation in the intracerebral group, but not in the intravenous, group. The BMSC were widely distributed in the ischemic brain and some of them expressed neural cell markers in the intracerebral group, but not in the intravenous group. These findings strongly suggest that intravenous administration of BMSC has limited effectiveness at clinically relevant timing and intracerebral administration should be chosen for patients with ischemic stroke, although further studies would be warranted to establish the treatment protocol.
  • 鴨嶋雄大, 寺坂俊介, 小山明彦, 蕨雄大, 下田祐介, 寳金清博
    Neurological Surgery 40 5 407 - 412 2012年05月10日 [査読有り][通常論文]
     
    We report a new simple method of cranial reconstruction using an autologous split calvarial bone, combined with free graft of temporal loose areolar tissue. A 58-year-old woman suffered from a cranium defect on her left side. The originating bone infection happened after initial brain tumor surgery. Part of the left side of her scalp just above the damaged cranial area had become very thin due to previous cranioplasty, which involved a titanium mesh plate and postoperative infections. We performed a cranial reconstruction with an autologous split calvarial bone, combined with loose areolar tissue free graft, for the damaged area with skin from the inner side. In our case, we expect that the addition of the free graft of loose areolar tissue to the autologous calvarial bone graft will effectively contribute to the skin's healing and provide good cosmetic results in our short follow-up period. A free graft of loose areolar tissue for the damaged skin area may be a new optional method for cranial reconstruction in a patient with skin trouble.
  • Kiyohiro Houkin, Masaki Ito, Taku Sugiyama, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Satoshi Kuroda
    NEUROLOGIA MEDICO-CHIRURGICA 52 5 267 - 277 2012年05月 [査読有り][通常論文]
     
    Research on moyamoya disease has progressed remarkably in the past several decades. Indeed, many new facts concerning the epidemiology of the disease have been revealed and surgical treatments have been drastically improved. However, despite extensive research, the mechanism of moyamoya disease is still unknown. Consequently, the cardinal treatment of this disease has not yet been developed. For further clarification of its etiology, innovative studies are therefore indispensable. The aim of this paper is to review research on the pathogenesis of moyamoya disease to identify milestones in the direction of its true solution. Many hypotheses of the pathogenesis of moyamoya disease have been proposed in the past half century, including infection (viral and bacterial), autoimmune disorders, proteins abnormality, and gene abnormality. Some of these are now considered to be historical achievements. Others, however, can be still subjected to contemporary research. Currently, several genetic abnormalities are considered to offer the most probable hypothesis. In addition, interesting papers have been presented on the role of the endothelial progenitor cell on the pathogenesis of moyamoya disease. Intuitively, however, it appears that a single theory cannot always explain the pathogenesis of this disease adequately. In other words, the complex mechanism of several factors may comprehensively explain the formation of moyamoya disease. The "double hit hypothesis" is probably the best explanation for the complicated pathology and epidemiology of this disease.
  • Satoshi Kuroda, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 52 5 287 - 294 2012年05月 [査読有り][通常論文]
     
    This review describes the basic concepts of surgical revascularization for moyamoya disease, including direct and indirect bypass surgery. Direct bypass surgery can improve cerebral hemodynamics and reduce further ischemic events immediately after surgery, but may be technically challenging in some pediatric patients. Indirect bypass surgery is simple and has widely been used. However, its beneficial effects can be achieved 3 to 4 months after surgery, and surgical design is quite important to determine the extent of surgical collateral pathways. Combined bypass procedure, especially superficial temporal artery (STA) to middle cerebral artery anastomosis and indirect bypass, encephalo-duro-myo-arterio-pericranial synangiosis, is a safe and effective option to improve the short- and long-term outcome in patients with moyamoya disease. Alternative techniques are also described for specific cases with profound cerebral ischemia in the anterior cerebral artery or posterior cerebral artery territory. Special techniques to safely complete bypass surgery and avoid perioperative complications are presented, including methods to prevent delayed wound healing, to avoid facial nerve palsy after surgery, and to preserve the STA and middle meningeal artery during skin incision and craniotomy. Finally, the importance of careful management of patients is emphasized to reduce the incidence of perioperative complications, including ischemic stroke and hyperperfusion syndrome.
  • Masaaki Hokari, Satoshi Kuroda, Yusuke Simoda, Haruto Uchino, Kenji Hirata, Tohru Shiga, Naoki Nakayama, Kiyohiro Houkin, Nagara Tamaki
    NEUROLOGIA MEDICO-CHIRURGICA 52 5 350 - 353 2012年05月 [査読有り][通常論文]
     
    Crossed cerebellar diaschisis (CCD) often occurs after ischemic or hemorrhagic stroke that damages the cortico-ponto-cerebellar pathway. However, CCD due to cerebral hyperperfusion following cerebrovascular reconstruction is rare. A 61-year-old woman presented with transient CCD due to cerebral hyperperfusion following bypass surgery for adult moyamoya disease. She developed transient weakness of the right extremities and was diagnosed with moyamoya disease. First, she underwent superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis with indirect synangiosis on the left. Postoperative course was uneventful. Subsequently, she underwent STA-MCA anastomosis with indirect synangiosis on the right. She complained of mild headache on the right, and single photon emission computed tomography (SPECT) performed on the 7th postoperative day demonstrated hyperperfusion in the right frontal and temporal lobes associated with hypoperfusion in the left cerebellum. Magnetic resonance (MR) imaging demonstrated no new lesions and MR angiography showed patent STA-MCA bypass. Subsequent SPECT showed disappearance of both hyperperfusion and CCD. This case strongly suggests that cerebral hyperperfusion after bypass surgery for moyamoya disease may cause transient CCD. Although the clinical significance is still obscure, this phenomenon indicates the cortico-ponto-cerebellar pathway is interrupted due to hyperperfusion, suggesting the development of hyperperfusion syndrome. Careful observation of cerebral hemodynamics after bypass surgery is warranted to avoid hyperperfusion-related complications.
  • 宮本倫行, 浅野剛, 長内俊也, 遠藤将吾, 中山若樹, 黒田敏, 寳金清博
    Neurological Surgery 40 5 415 - 20 2012年05月 [査読有り][通常論文]
     
    The authors reported a subclavian artery stenting (SAS) using gadolinium contrast medium. The patient was a 65-year-old female who presented dizziness and right upper extremity pain with movement. Digital subtraction angiography revealed right subclavian artery occlusion with subclavian steal phenomenon. We tried to treat this lesion using SAS. However, iodinated contrast medium caused the allergy in this patient and the treatment was discontinued. Therefore, SAS was performed with gadolinium contrast medium. Using gadolinium contrast medium, it is possible to confirm large arteries like innominate artery and subclavian artery. The stenting procedure was performed without complication. The usage of gadolinium contrast medium has the limit and some strategies are important to reduce the usage of gadolinium contrast medium in SAS. First, PercuSurge GuardWire® was placed in the right internal carotid artery to confirm the anatomy, to decide working angle, and to treat the common carotid artery in case of dissection. Second, a "U" shaped guide wire was placed in the distal end from the brachial artery. Guide wire from femoral side was able to pass the lesion at midpoint of the "U" shaped one. SAS using gadolinium contrast medium may be an alternative treatment if a patient with subclavian artery stenosis or occlusion is allergic to iodinated contrast medium.
  • Hirata K, Terasaka S, Shiga T, Hattori N, Magota K, Kobayashi H, Yamaguchi S, Houkin K, Tanaka S, Kuge Y, Tamaki N
    European journal of nuclear medicine and molecular imaging 39 5 760 - 770 5 2012年05月 [査読有り][通常論文]
     
    Purpose Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor and its prognosis is significantly poorer than those of less malignant gliomas. Pathologically, necrosis is one of the most important characteristics that differentiate GBM from lower grade gliomas; therefore, we hypothesized that F-18 fluoromisonidazole (FMISO), a radiotracer for hypoxia imaging, accumulates in GBM but not in lower grade gliomas. We aimed to evaluate the diagnostic value of FMISO positron emission tomography (PET) for the differential diagnosis of GBM from lower grade gliomas. Methods This prospective study included 23 patients with pathologically confirmed gliomas. All of the patients underwent FMISO PET and 18F-fluorodeoxyglucose (FDG) PET within a week. FMISO images were acquired 4 h after intravenous administration of 400 MBq of FMISO. Tracer uptake in the tumor was visually assessed. Lesion to normal tissue ratios and FMISO uptake volume were calculated. Results Of the 23 glioma patients, 14 were diagnosed as having GBM (grade IV glioma in the 2007 WHO classification), and the others were diagnosed as having non-GBM (5 grade III and 4 grade II). In visual assessment, all GBM patients showed FMISO uptake in the tumor greater than that in the surrounding brain tissues, whereas all the non-GBM patients showed FMISO uptake in the tumor equal to that in the surrounding brain tissues (p <= 0.001). One GBM patient was excluded from FDG PET study because of hyperglycemia. All GBM patients and three of the nine (33%) non-GBM patients showed FDG uptake greater than or equal to that in the gray matter. The sensitivity and specificity for diagnosing GBM were 100 and 100% for FMISO, and 100 and 66% for FDG, respectively. The lesion to cerebellum ratio of FMISO uptake was higher in GBM patients (2.74 +/- 0.60, range 1.71-3.81) than in non-GBM patients (1.22 +/- 0.06, range 1.09-1.29, p <= 0.001) with no overlap between the groups. The lesion to gray matter ratio of FDG was also higher in GBM patients (1.46 +/- 0.75, range 0.91-3.79) than in non-GBM patients (1.07 +/- 0.62, range 0.66-2.95, p <= 0.05); however, overlap of the ranges did not allow clear differentiation between GBM and non-GBM. The uptake volume of FMISO was larger in GBM ( 27.18 +/- 10.46%, range 14.02- 46.67%) than in non- GBM ( 6.07 +/- 2.50%, range 2.12- 9.22%, p <= 0.001). Conclusion These preliminary data suggest that FMISO PET may distinguish GBM from lower grade gliomas.
  • Norihiro Suzuki, Motoki Sato, Kiyohiro Houkin, Yasuo Terayama, Shinichiro Uchiyama, Hiroyuki Daida, Hiroshi Shigematsu, Shinya Goto, Kortaro Tanaka, Hideki Origasa, Susumu Miyamoto, Kazuo Minematsu, Masayasu Matsumoto, Yasushi Okada
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 21 4 245 - 253 2012年05月 [査読有り][通常論文]
     
    Background: Patients with recent ischemic stroke may have higher risk of atherothrombosis than stable patients with established vascular events. Our aims were to investigate 1-year atherothrombotic vascular event rates and to assess the risk factors for recurrent ischemic stroke in this population. Methods: This prospective cohort study was conducted between January 2007 and July 2009 at 313 hospitals in Japan. Outpatients who were at least 45 years of age and who had received oral antiplatelet therapy were enrolled within 2 weeks to 6 months from the last onset of noncardioembolic ischemic stroke. At 12 +/- 3 months after enrollment, data on presence/absence of atherothrombotic vascular events were collected. The primary endpoint was the occurrence of fatal or nonfatal ischemic stroke. Results: A total of 3452 patients were enrolled, and 3411 patients who had baseline data were included in the analysis. The 1-year event rate was 3.81% (9.5% confidence interval 3.15-4.48%) for fatal or nonfatal ischemic stroke and 0.84% (95% confidence interval 0.52-1.15%) for all-cause mortality. The annual rate of recurrent ischemic stroke was significantly higher in patients who had ischemic stroke at least twice than in patients who had first-ever ischemic stroke (5.02% vs 3.59%; P = .0313). In the multivariable Cox regression analysis, recurrent ischemic stroke was significantly associated with age (P = .0033), the presence of diabetes (P = .0129), and waist circumference >= 80 cm (P = .0056). Conclusions: Patients with recent ischemic stroke have a higher risk of stroke recurrence than stable patients enrolled in the REduction of Atherothrombosis for Continued Health (REACH) registry even though they received antiplatelet therapy. The rigorous management of risk factors is needed.
  • Kiyohiro Houkin, Masaki Ito, Michiyuki Miyamoto, Masaaki Hokari, Ken Kazumata, Naoki Nakayama, Satoshi Kuroda
    Neurological Surgery 40 4 365 - 375 2012年04月10日 [査読有り][通常論文]
  • 寳金清博, 伊東雅基, 宮本倫行, 穂刈正昭, 数又研, 中山若樹, 黒田敏
    Neurol Surg 40 4 365-375  2012年04月10日 [査読無し][通常論文]
  • Shigeru Yamaguchi, Hiroyuki Kobayashi, Shunsuke Terasaka, Nobuaki Ishii, Jun Ikeda, Hiromi Kanno, Hiroshi Nishihara, Shinya Tanaka, Kiyohiro Houkin
    JAPANESE JOURNAL OF CLINICAL ONCOLOGY 42 4 270 - 277 2012年04月 [査読有り][通常論文]
     
    We reviewed the relationship between extent of resection and survival of patients with high-grade gliomas with special consideration of an oligodendroglial component.A retrospective review was performed on 160 adult patients with histological diagnosis of high-grade gliomas since 2000. All histological slides were categorized as high-grade astrocytomas or oligodendroglial tumors. Extent of resection was assessed by early post-operative magnetic resonance imaging and classified as complete resection, incomplete resection and biopsy. Measured outcomes were overall survival and progression-free survival. The independent association of extent of resection and survival was analyzed by the multivariate proportional hazard model adjusting for prognostic factors.The lesions were classified as high-grade astrocytomas in 93 patients and high-grade oligodendroglial tumors in 67 patients. In high-grade astrocytomas, the median survival after complete resection (n 36), incomplete resection (n 36) and biopsy (n 21) was 23.4, 15.3 and 12.6 months, respectively. Complete resection was independently associated with increased overall survival (P 0.001) and progression-free survival (P 0.002) compared with incomplete resection, while incomplete resection was not associated with survival benefit compared with biopsy by multivariate analysis. On the other hand, in high-grade oligodendroglial tumors, the majority of patients were still alive and there is no significant difference in the survival between complete resection (n 24) and incomplete resection (n 33), while even incomplete resection had a significantly longer overall survival (P 0.001) and progression-free survival (P 0.006) compared with biopsy (n 10).Maximal cytoreduction improves the survival of high-grade gliomas, although our data indicated that the impact of extent of resection in high-grade astrocytomas is different from that in high-grade oligodendroglial tumors.
  • Yasuhiro Chiba, Satoshi Kuroda, Toshiya Osanai, Hideo Shichinohe, Kiyohiro Houkin, Yoshinobu Iwasaki
    Neuropathology : official journal of the Japanese Society of Neuropathology 32 2 139 - 48 2012年04月 [査読有り][通常論文]
     
    This study was designed to clarify the effects of donor age on biological features of bone marrow stromal cells (BMSC), one of the candidates for cell transplantation therapy for CNS disorders, because many aged patients might require such therapy. This study was also aimed to test whether ex vivo treatments with granulocyte-colony stimulating factor (G-CSF) could modify biological properties of BMSC from aged donors and enhance its therapeutic effects in an animal model of traumatic brain injury. The BMSC were harvested from young (6-week-old) and aged (100-week-old) rats. The ageing significantly increased the senescence-associated β-galactosidase (SA-β-gal) activity of the cultured BMSC, and decreased their proliferative capacity and production of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). As the next step, the rats were subjected to brain freezing injury by applying liquid nitrogen onto the neocortex through the thinned skull. The 6-week BMSC, 100-week BMSC, G-CSF-treated 100-week BMSC or vehicle were stereotactically injected into the ipsilateral striatum at 7 days post-injury. Transplantation of the 6-week BMSC, but not 100-week BMSC, significantly improved locomotor function. However, treatment of the 100-week BMSC with 0.1 µmol of G-CSF significantly improved their proliferation activity and growth factor production, and recovered therapeutic effects in the injured brain. In conclusion, donor age may largely determine biological aspects of BMSC. G-CSF may contribute to improve the outcome of BMSC transplantation therapy for CNS disorders in aged patients.
  • 鴨嶋雄大, 寺坂俊介, 下田祐介, 小林浩之, 黒田敏, 浅野剛, 山口秀, 村田純一, 寳金清博
    Neurological Surgery 40 3 229 - 234 2012年03月10日 [査読有り][通常論文]
     
    Hemangioblastoma in the medulla oblongata is a relatively rare tumor. We present the case of a giant hemangioblastoma occurring in the dorsal medulla oblongata. A 33-year-old man with no neurological symptoms was diagnosed with a hemangioblastoma in the dorsal medulla oblongata, and opted for observation in the outpatient department. After 22 months of observation time, MRI scans showed rapid local tumor progression and obstructive hydrocephalus. At this point, he presented with mild dysphagia as a preoperative neurological deficit. Total surgical removal of the tumor was performed after temporary ventricle drainage and preoperative embolization of the feeding artery. Postoperatively, he became fully conscious but developed bulbar palsy followed by tracheostomy. During the 12 months of postoperative follow-up, severe dysphagia was still present.
  • 淺野剛, 寳金清博, 森脇拓也, 新谷好正, 馬渕正二
    Neurological Surgery 40 3 235 - 239 2012年03月10日 [査読有り][通常論文]
     
    We report a case of longstanding asymptomatic direct carotid-cavemous fistula (CCF) which caused fatal subarachnoid hemorrhage (SAH). A 91-year-old female with no history of previous head trauma and optic symptoms presented acute subarachnoid hemorrhage. Angiography revealed a left direct carotid-cavernous fistula draining only into the contralateral cavernous sinus with leptomeningeal venous reflux and small varix on the pontine bridging vein. The affected cavernous sinus was markedly dilated and there was no septum between the left cavernous sinus and the internal carotid artery. The patient underwent transvenous coil embolization for intercavernous sinus and leptomeningeal venous reflux was successfully obliterated and opacification of the varix was diminished. The past history of this patient and angiographical findings strongly suggest long standing asymptomatic CCF caused SAH.
  • Hongyan Han, Satoshi Kuroda, Yusuke Shimoda, Kiyohiro Houkin
    JOURNAL OF CHILD NEUROLOGY 27 3 408 - 413 2012年03月 [査読有り][通常論文]
     
    Among patients with moyamoya disease, familial occurrence is observed in about 20%, suggesting the involvement of genetic factors. In this report, we describe the first predictive case of moyamoya disease in a boy born to a woman who underwent surgical revascularization for moyamoya disease when she was 3 years old. We educated the mother and her family not to miss his initial signs of the disease. His family could easily notice his brief episode of ischemic attack when he was 6 years old. He underwent superficial temporal artery-to-middle cerebral artery anastomosis and indirect bypass on both sides. The postoperative course was uneventful. In conclusion, it is quite important to educate the family not to miss the initial signs of disease in their offspring, at least when they have a genetic background of the disease, because early diagnosis and effective treatment are essential to improve the long-term outcome in pediatric patients.
  • 三上 毅, 杉野 寿哉, 菅野 彩, 宝金 清博, 三國 信啓
    脳卒中 34 2 82 - 88 (一社)日本脳卒中学会 2012年03月 [査読無し][通常論文]
     
    著者らは2007年1月〜2010年12月にかけ甲状腺機能亢進症に併発した類もやもや病4例(男性2例、女性2例、年齢14〜43歳、平均年齢27.5歳)に対し血行再建術を行った。今回、その病態や治療方針について検討した結果、1)脳血管撮影所見やMRA所見では一定の傾向はみられなかった。2)手術は直接血行再建術と間接血行再建術が行われ、TIAは全例で消失し、1例で認められた舞踏病様の不随意運動も消失した。だが、1例では甲状腺機能の悪化みられた。
  • Hideo Shichinohe, Satoshi Kuroda, Kohsuke Kudo, Masaki Ito, Masahito Kawabori, Michiyuki Miyamoto, Mitsuhiro Nakanishi, Satoshi Terae, Kiyohiro Houkin
    Translational Stroke Research 3 1 99 - 106 2012年03月 [査読有り][通常論文]
     
    Recent studies have elucidated that transplantation of the bone marrow stromal cells (BMSC) has therapeutic potential for the central nervous system (CNS) disorders. However, no imaging modalities have been established to track the engrafted cells in the CNS in clinical situation. This study aimed to investigate the ability of magnetic resonance imaging (MRI) to visualize the BMSC labeled with superparamagnetic iron oxide (SPIO). The BMSC of mice were labeled with SPIO. Various numbers of the cells were injected into the agar phantom and were visualized using a 3. 0-T MR apparatus. The SPIO-labeled cells were injected into the temperature-sensitive gelation polymer (TGP) hydrogel and were cultured for 7 days. They were also visualized just after the injection and at 7 days postinjection. After a 7-day culture, they were stained with Turnbull blue technique. T2-, T2*-, and susceptibility-weighted imaging could identify minimally 1,000 cells in the agar or TGP hydrogel, although it was difficult to quantify their number on MRI. All of these sequences could track the SPIO-labeled BMSC for at least 7 days when injected into the TGP. Turnbull blue staining revealed the survival and proliferation of the SPIO-labeled BMSC in the TGP for 7 days. The findings strongly suggest that the SPIO labeling may enable to track minimally 1,000 cells engrafted in the CNS on clinical MR apparatus. These data would be valuable to consider the application of imaging technique into cell transplantation therapy for CNS disorders. © 2011 Springer Science+Business Media, LLC.
  • 鴨嶋雄大, 茂木洋晃, 寺坂俊介, 小林浩之, 山口秀, 村田純一, 田中伸哉, 寳金清博
    Neurological Surgery 40 2 129 - 135 2012年02月10日 [査読有り][通常論文]
     
    Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with an extremely poor prognosis in spite of multimodal treatment approaches. The median survival time of patients with GBM is 15 months, and only 3-5% of patients survive longer than 36 months. Those patients who survive over 36 months after the initial diagnosis are defined as long-term survivors. In this study, we retrospectively performed clinical and molecular analyses of five long-term survivors of GBM (> 36 months) and twenty four GBM patients with poor survival time as control group (< 36 months) to identify any prognostic factors that potentially contribute to survival. The O 6- methylguanine-DNA methyltransferase (MGMT) gene methylation status was evaluated by performing methylation specific polymerase chain reaction assays. The mutation of isocitrate dehydrogenase 1 and 2 were evaluated by the direct sequencing method. All five cases were primary GBMs and the coexistence of the oligodendroglioma component was checked for each case as GBM with oligodendroglioma component. All five cases showed MGMT promoter methylation (5/5). IDH1 mutation was detected in two of the long-term survivors with oligodendroglioma component (2/5) while no IDH1 mutation was detected in the control group. All patients were treated by gross total removal followed by radiotherapy and various chemotherapies including temozolomide. MGMT promoter methylation and IDH1 mutation might be favorable factors for long-term survival in GBM patients.
  • Takuhito Narita, Hidefumi Aoyama, Kenji Hirata, Shunsuke Onodera, Tohru Shiga, Hiroyuki Kobayashi, Junichi Murata, Shunsuke Terasaka, Shinya Tanaka, Kiyohiro Houkin
    JAPANESE JOURNAL OF CLINICAL ONCOLOGY 42 2 120 - 123 2012年02月 [査読有り][通常論文]
     
    Two glioblastoma multiforme patients underwent F-18-FMISO (fluoromisonidazole) positron emission tomography study to access the tumor oxygenation status before and immediately after fractionated radiotherapy concomitant with temozolomide chemotherapy. In both cases, a prominent F-18-FMISO tumor accumulation observed in the first study was notably decreased in the second study, which was supposed to be a reoxygenation of the tumor. As far as we investigated, this is the first report of the changes of oxygenation status in glioblastoma multiforme treated through radiation therapy with temozolomide.
  • Toshiya Osanai, Satoshi Kuroda, Taku Sugiyama, Masahito Kawabori, Masaki Ito, Hideo Shichinohe, Yuji Kuge, Kiyohiro Houkin, Nagara Tamaki, Yoshinobu Iwasaki
    Neurosurgery 70 2 435 - 44 2012年02月 [査読有り][通常論文]
     
    BACKGROUND: A noninvasive and effective route of cell delivery should be established to yield maximal therapeutic effects for central nervous system (CNS) disorders. OBJECTIVE: To elucidate whether intra-arterial delivery of bone marrow stromal cells (BMSCs) significantly promotes functional recovery in traumatic brain injury (TBI) in rats. METHODS: Rat BMSCs were transplanted through the ipsilateral internal carotid artery 7 days after the onset of cortical freezing injury. The BMSCs were labeled with fluorescent dye, and in vivo optical imaging was employed to monitor the behaviors of cells for 4 weeks after transplantation. Motor function was assessed for 4 weeks, and the transplanted BMSCs were examined using immunohistochemistry. RESULTS: In vivo optical imaging and histologic analysis clearly demonstrated that the intra-arterially injected BMSCs were engrafted during the first pass without systemic circulation, and the transplanted BMSCs started to migrate from the cerebral capillary bed to the injured CNS tissue within 3 hours. Intra-arterial BMSC transplantation significantly promoted functional recovery after cortical freezing injury. A subgroup of BMSCs expressed the phenotypes of neurons, astrocytes, and endothelial cells around the injured neocortex 4 weeks after transplantation. CONCLUSION: Intra-arterial transplantation may be a valuable option for prompt, noninvasive delivery of BMSCs to the injured CNS tissue, enhancing functional recovery after TBI. In vivo optical imaging may provide important information on the intracerebral behaviors of donor cells by noninvasive, serial visualization.
  • Yuuta Kamoshima, Shunsuke Terasaka, Yuji Nakamaru, Dai Takagi, Satoshi Fukuda, Kiyohiro Houkin
    Case Reports in Neurology 4 1 34 - 37 2012年01月 [査読有り][通常論文]
     
    Giant mucoceles of the frontal sinus are rare but their recognition is important in the differential diagnosis of proptosis and fronto-orbital lesions. The authors describe a patient with frontal giant mucocele with intracranial as well as orbit and ethmoid sinus involvement. Thirty-two years after a frontal sinus fracture, a 51-year-old female presented with headache, and left exophthalmos and ophthalmoplegia. Computed tomography and magnetic resonance imaging demonstrated a giant frontal sinus mucocele with extension into the left anterior cranial fossa. The mucocele was treated with a transcranial and endoscopic transnasal approach. The frontal sinus was then cranialized with reconstruction of the posterior wall, and finally a wide nasal drainage was performed. The clinical symptoms disappeared immediately after surgery. Copyright © 2012 S. Karger AG, Basel.
  • Shunsuke Terasaka, Masahito Kawabori, Hiroyuki Kobayashi, Junichi Murata, Hiromi Kanno, Shinya Tanaka, Kiyohiro Houkin
    BRAIN TUMOR PATHOLOGY 29 1 58 - 62 2012年01月 [査読有り][通常論文]
     
    We report an unusual case of neurohypophyseal germinoma with abundant fibrous tissue and clival invasion that was initially misdiagnosed as lymphocytic hypophysitis. A 40-year-old woman presented with diabetes insipidus and panhypopituitarism after delivering her second son and which lasted for 4 years. Magnetic resonance imaging showed the intrasellar mass extending to the suprasellar region with enlarged pituitary stalk. The mass was heterogeneously enhanced and invaded the clivus. Biopsy of the intrasellar mass was performed via the trans-sphenoidal route, and histological examination revealed marked fibrous tissue and infiltration of lymphocytes, with no evidence of tumor cells. Lymphocytic hypophysitis was the initial diagnosis, and corticosteroid therapy was begun. Despite intensive treatment, the lesion enlarged and clinical symptoms worsened 2 weeks after surgery. Subtotal removal of the mass was performed, and a second histological examination revealed typical findings of the germinoma. Subsequently, the patient underwent chemoradiotherapy, and complete remission was achieved. Histological diagnosis is sometimes incorrect in fibrous tumors at the sellar region, and biopsy from several points is strongly recommended for this entity.
  • Shimoda Yusuke, Nakayama Naoki, Hokari Masaaki, Shitinohe Hideo, Kuroda Satoshi, Hokin Kiyohiro, Sakakibara Mamoru, Tsutsui Hiroyuki, Ooka Tomonori, Matsui Yoshiro
    CEREBROVASCULAR DISEASES 34 109 - 110 2012年 [査読有り][通常論文]
  • Takeo Abumiya, Masahito Katoh, Takuya Moriwaki, Masami Yoshino, Takeshi Aoki, Hiroyuki Imamura, Toshimitsu Aida, Naoki Nakayama, Kiyohiro Houkin
    CEREBROVASCULAR DISEASES 34 5-6 446 - 447 2012年 [査読有り][通常論文]
  • 下田祐介, 黒田敏, 小山明彦, 浜内祝嗣, 山崎和義, 寳金清博
    小児の脳神経 37 2 2012年
  • 七戸秀夫, 黒田敏, 山内朋裕, 齋藤久泰, 新保大輔, 寳金清博
    脳循環代謝 24 1 2012年
  • 七戸秀夫, 石原武, 黒田敏, 高橋浩治, 田中嘉一, 宮本倫行, 山内朋裕, 斎藤久泰, 武本浩, 寳金清博
    再生医療 11 2012年
  • Shimbo Daisuke, Kuroda Satoshi, Nakayama Naoki, Kazumata Ken, Hirata Kenji, Shiga Tohru, Houkin Kiyohiro, Tamaki Nagara
    CEREBROVASCULAR DISEASES 34 19  2012年 [査読有り][通常論文]
  • Nakayama Naoki, Houkin Kiyohiro, Ishikawa Tatsuya, Hokari Masaaki, Kazumata Ken, Ito Masaki, Sugiyama Taku, Yasuda Hiroshi
    CEREBROVASCULAR DISEASES 34 20  2012年 [査読有り][通常論文]
  • Kazumata Ken, Houkin Kiyohiro, Nakayama Naoki, Shichinohe Hideo, Osanai Toshiya, Shinpo Daisuke, Saito Hisayasu
    CEREBROVASCULAR DISEASES 34 32  2012年 [査読有り][通常論文]
  • Osanai Toshiya, Ushikoshi Satoshi, Ken Kazumata, Nakayama Naoki, Houkin Kiyohiro
    CEREBROVASCULAR DISEASES 34 92  2012年 [査読有り][通常論文]
  • Ito Masaki, Niiya Yoshimasa, Uchino Haruto, Itosaka Hiroyuki, Shichinohe Hideo, Kazumata Ken, Nakayama Naoki, Mabuchi Syoji, Houkin Kiyohiro
    CEREBROVASCULAR DISEASES 34 102  2012年 [査読有り][通常論文]
  • 骨髄間質細胞移植は脳梗塞周囲の神経受容体機能を改善させる―123I‐iomazenil SPECTによる検討
    齋藤久泰, 黒田敏, 宮本倫行, 山内朋裕, 七戸秀夫, 趙松吉, 孫田惠一, 久保直樹, 久下裕司, 玉木長良, 寶金清博
    脳循環代謝 24 1 216  2012年 [査読無し][通常論文]
  • 脳梗塞をターゲットとした骨髄間質細胞移植―新たな培養・移植・イメージング技術による展開
    黒田敏, 宮本倫行, 山内朋裕, 斉藤久泰, 伊東雅基, 川堀真人, 杉山拓, 千葉泰弘, 七戸秀夫, 宝金清博, 久下裕司, 趙松吉, 玉木長良
    再生医療 11 136  2012年 [査読無し][通常論文]
  • Hiroyuki Kobayashi, Masahito Kawabori, Shunsuke Terasaka, Junichi Murata, Kiyohiro Houkin
    ACTA NEUROCHIRURGICA 153 12 2453 - 2456 2011年12月 [査読有り][通常論文]
     
    Isolated oculomotor nerve palsy occasionally occurs in patients with cavernous sinus invasion with or without pituitary apoplexy. We describe two cases of pituitary apoplexy without cavernous sinus invasion presenting with isolated oculomotor palsy. In both cases, computed tomography (CT) showed erosion of the right posterior clinoid process. Magnetic resonance imaging (MRI) depicted pituitary adenoma with apoplexy protruding latero-posteriorly to the right cavernous sinus. The medio-posterior wall of the cavernous sinus was markedly displaced latero-posteriorly by the tumor, and there was no evidence of cavernous sinus invasion. Oculomotor palsy may be caused first by unilateral erosion of the posterior clinoid process, resulting in latero-posterior protrusion of the adenoma. Hemorrhage may result in sudden kinking of the oculomotor nerve at the entrance of the oculomotor trigone.
  • 伊師雪友, 伊東雅基, 寺坂俊介, 茂木洋晃, 新保大輔, 金子貞洋, 寳金清博
    Neurological Surgery 39 11 1061 - 1066 2011年11月10日 [査読有り][通常論文]
     
    Complications arising from the placement of ventriculoperitoneal shunts are common. These complications may be related to a number of causes and present with various symptoms. Of these, abdominal complications such as formation of intraperitoneal pseudocysts and abdominal abscesses possibly recur, but, alternative sites for placing the peritoneal catheter of ventriculoperitoneal shunts are limited. We present two cases of ventriculoperitoneal shunt malfunctioning due to repeated abdominal complications. The location of the peritoneal end of the shunt was successfully revised to the suprahepatic space in the peritoneal cavity. We describe the clinical course of these two cases in this report, along with a precise technique of placing the peritoneal end of the shunt into the suprahepatic space. In addition, we will discuss the validity of this space as an alternative site for the placement of the peritoneal end of the ventriculoperitoneal shunt.
  • 栗栖宏多, 飛騨一利, 青山剛, 寳金清博
    Neurological Surgery 39 11 1079 - 1084 2011年11月10日 [査読有り][通常論文]
     
    Idiopathic syringomyelia not associated with Chiari malformation, tumor, nor tetherd cord syndrome has not been frequently reported. So the clinical significance and optimum treatment strategy for this disease remains unclear. We report a 37-year-old man who was diagnosed as having idiopathic syringomyelia and was treated successfully using implantation of a syringo-subarachnoid shunt. The patient suffered gradual worsening of sensory disturbance in the upper extremities, and Magnetic resonance imaging demonstrated a large syrinx located in the cervical spinal cord (C2-3), located mainly at the left anterior horn. We performed syringo-subarachnoid shunt for syringomyelia under partial hemilaminectomy. During the surgery, the spinal cord was markedly rotated and dorsal root entry had shifted to the midline because of the large syrinx. In order to perform myelotomy at the dorsal root entry zone, we needed additional drilling to the medial portion of the lamina. After this procedure, we were able to perform implantation of the syringo-subarachnoid shunt safely. After the operation, the syrinx collapsed immediately and no neurological deficit was observed. The patient was discharged 2 weeks after the operation. Idiopathic syringomyelia in which the syrinx has deviated to the anterior horn is rare. In those cases, we consider that sufficient hemilaminectomy for the midline should be performed.
  • 鴨嶋雄大, 寺坂俊介, 下田祐介, 福田聡, 寳金清博
    Neurological Surgery 39 11 1099 - 1103 2011年11月10日 [査読有り][通常論文]
     
    Hemifacial spasm is a movement disorder characterized by involuntary paroxysmal chronic contractions of the facial musculature. The usual cause is simple vascular compression of the facial nerve, at its root exit zone of the brain stem. Previously only a case of hemifacial spasm associated with a juglar foramen tumor has been reported in the literature. In this article, we report a case in which hemifacial spasm accompanied an ipsilateral juglar foramen tumor in a 62-year-old woman. The sole use of arterial decompression of the facial nerve at the root exit zone resulted in complete resolution of the patient's symptoms.
  • Taku Sugiyama, Satoshi Kuroda, Naoki Nakayama, Shinya Tanaka, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 51 11 767 - 773 2011年11月 [査読有り][通常論文]
     
    The mechanisms through which moyamoya disease occurs and progresses remain unknown. Recent studies have indicated the involvement of circulating endothelial progenitor cells (EPCs) in the development of moyamoya disease. This study directly investigated the participation of EPCs in moyamoya disease, using specimens of the supraclinoid internal carotid artery collected from two adult patients. The specimens were stained with primary antibodies against CD34, CD133, and vascular endothelial growth factor receptor-2 (VEGFR2) to localize the circulating EPCs in the thickened intima of occlusive arterial lesion. The CD34- and VEGFR2-positive cells were densely found in the thickened intima of occlusive arterial lesion, particularly clustered in the superficial layer of thickened intima. However, the number of CD34- and CD133-positive cells was very small. The CD34-positive cells also expressed von Willebrand factor on the surface of thickened intima and were also positive for a-smooth muscle actin in the deeper layer. These findings suggest that circulating EPCs may be involved in the development of occlusive arterial lesion in moyamoya disease.
  • Shigeru Yamaguchi, Shunsuke Terasaka, Hiroyuki Kobayashi, Katsuyuki Asaoka, Junichi Murata, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 51 11 789 - 792 2011年11月 [査読有り][通常論文]
     
    A 43-year-old female presented with a giant skull base atypical meningioma manifesting as rapid progression of impaired consciousness. The meningioma was located in the ethmoid sinus, sphenoid sinus, nasal cavity, and left middle temporal fossa, and the intracranial portion of the tumor involved the left temporal region with massive surrounding brain edema in the left temporal lobe and basal ganglia. She underwent emergent fronto-temporo-parietal decompressive craniectomy, and the intracranial portion of the tumor was resected to control intracranial pressure. She recovered consciousness and neurological function dramatically, and subsequently underwent radical tumor resection via combined extended transbasal and left lateral transzygomatic infratemporal fossa approaches one month after the initial surgery. The extensive brain edema completely disappeared after tumor resection, and the patient fully recovered without neurological deficits except anosmia and small visual field defect. Rapid neurological deterioration and disturbance of consciousness caused by extensive peritumoral brain swelling are unusual in meningioma. In this case, the extemporaneous decompressive craniectomy was highly useful in the management of increased intracranial pressure.
  • KAMOSHIMA Yuuta, SAWAMURA Yutaka, IMAI Tetsuaki, FURUKAWA Hiroshi, KUBOTA Kanako, HOUKIN Kiyohiro
    Neurologia medico-chirurgica = 神経外科 51 11 798 - 800 一般社団法人 日本脳神経外科学会 2011年11月 [査読有り][通常論文]
     
    A 2-year-old female presented with a rare case of recurrent giant cell tumor affecting the frontal bone. She had already undergone partial removal twice at the ages of 14 and 18 months. The tumor was located in the frontal bone, expanding to the ethmoid and orbital bones, and invading the frontal base dura mater. The tumor was totally removed including the surrounding bone and frontal base dura mater. No local recurrence and metastasis were observed at 18 months after the last operation. Most giant cell tumors occur in the epiphyses of long bones and are rare in the cranio-facial bone. These tumors usually affect young adults and few pediatric cases are reported.
  • 川堀 真人, 黒田 敏, 中山 若樹, 石川 達哉, 宝金 清博
    脳循環代謝 23 1 155 - 155 (一社)日本脳循環代謝学会 2011年11月 [査読無し][通常論文]
  • 伊東 雅基, 黒田 敏, 穂刈 正昭, 中山 若樹, 志賀 哲, 玉木 長良, 宝金 清博
    脳循環代謝 23 1 117 - 117 (一社)日本脳循環代謝学会 2011年11月 [査読無し][通常論文]
  • 内野 晴登, 黒田 敏, 中山 若樹, 宝金 清博
    脳循環代謝 23 1 157 - 157 (一社)日本脳循環代謝学会 2011年11月 [査読無し][通常論文]
  • 新保 大輔, 黒田 敏, 中山 若樹, 宝金 清博
    脳循環代謝 23 1 123 - 123 (一社)日本脳循環代謝学会 2011年11月 [査読無し][通常論文]
  • 宮本 倫行, 長内 俊也, 浅野 剛, 中山 若樹, 黒田 敏, 宝金 清博
    JNET: Journal of Neuroendovascular Therapy 5 4 219 - 219 (NPO)日本脳神経血管内治療学会 2011年11月 [査読無し][通常論文]
  • 骨髄間質細胞移植は脳梗塞後の局所糖代謝を改善する 小動物用PET/CTによる検討
    宮本 倫行, 黒田 敏, 七戸 秀夫, 伊東 雅基, 川堀 真人, 趙 松吉, 孫田 恵一, 久下 裕司, 玉木 長良, 宝金 清博
    脳循環代謝 23 1 142 - 142 (一社)日本脳循環代謝学会 2011年11月 [査読無し][通常論文]
  • 栗栖宏多, 鴨嶋雄大, 寺坂俊介, 小林浩之, 久保田佳奈子, 寳金清博
    Neurological Surgery 39 10 991 - 997 2011年10月10日 [査読有り][通常論文]
     
    Metastatic intraventricular tumor located in the choroid plexus is very rare. Only a few cases have been reported in the past. According to past reports, these tumors originated from lung, colon, and so on, but not from the bile duct. This is the first case report of choroid plexus metastasis from cholangiocellular carcinoma. A 57-year-old woman who had a history of cholagiocellular carcinoma, demonstrated intraventricular tumor. Although sufficient examination was performed, the tumor was difficult to diagnose as being a metastatic tumor or a choroid plexus carcinoma. Because of this, we performed endoscopic biopsy of the intraventricular tumor. However intraoperative findings were not helpful in distinguishing metastatic tumor and choroid plexus carcinoma. Postoperatively, histological examination was performed. However it was still difficult to differentiate this rare tumor from choroid plexus carcinoma by only hematoxylin and eosin stain. For further examination, Ber EP-4 stain was performed. Ber EP-4 showed strongly positive which indicates metastatic tumor. This method led us to make an appropriate diagnosis of this extremely rare tumor. We considered that in order to diagnose this rare tumor, appropriate histopathological examination, including immunohistopathological examination should be performed.
  • 鴨嶋雄大, 寺坂俊介, 浜内祝嗣, 小林浩之, 遠藤将吾, 菅野由岐子, 来海公彦, 寳金清博
    Neurological Surgery 39 10 999 - 1004 2011年10月10日 [査読有り][通常論文]
     
    Symptomatic metastases to the pituitary from renal cell carcinoma are rare. We present a case of pituitary metastases from renal cell carcinoma showing panhypopituitarism. A 50-year-old man who had renal cell carcinoma with distant metastases in skin, bone and lymph nodes was referred to our department. Clinically he showed severe cognitive function disorder. Endocrinological evaluation revealed central adrenal and gonadal insufficiencies. Brain magnetic resonance imaging demonstrated a hemorrhagic mass in left frontal lobe and a sellar mass with supra sellar cistern extension. After hormonal replacement and surgical removal of the frontal tumor, he immediately recovered from his cognitive function disorder. Subsequently, whole brain radiotherapy for metastatic pituitary tumor was performed. At present, he is being treated with molecular targeting drugs for other distant metastases and he presents no neurological deficit. Palliative therapy for CNS metastases from renal cell carcinoma may result in better quality of life for patients with advanced stage of renal cell carcinoma.
  • KAMOSHIMA Yuuta, SAWAMURA Yutaka, MOTEGI Hiroaki, KUBOTA Kanako, HOUKIN Kiyohiro
    Neurologia medico-chirurgica = 神経外科 51 10 689 - 693 一般社団法人 日本脳神経外科学会 2011年10月 [査読有り][通常論文]
     
    Five Japanese children presented with rare xanthogranuloma located in the sellar region between 2000 and 2010 at the Department of Neurosurgery, Hokkaido University Hospital. Endocrinological examination disclosed central diabetes in four patients. Preoperative magnetic resonance (MR) imaging and computed tomography (CT) demonstrated clearly defined intra- or suprasellar masses appearing as isointense or hyperintense on T1-weighted MR imaging with no calcification on CT. The tumor was totally removed under preoperative diagnosis of craniopharyngioma in all cases. Histological examination found fibrous tissue with abundant cholesterol clefts and hemosiderin deposits, but no or only tiny amounts of epithelial cells in each case. Therefore, the histological diagnoses were xanthogranuloma of the sellar region, not adamantinomatous craniopharyngioma. Postoperatively, no patient recovered from endocrinological deficits, whereas visual disturbances were improved immediately after operation. Currently, whether xanthogranuloma is distinct from adamantinomatous craniopharyngioma remains unclear. Only a few clinical reports of xanthogranuloma of the sellar region have been reported in pediatric patients. Our series shows that xanthogranuloma should be included in the differential diagnosis of pediatric tumor of the sellar region.
  • Masahiko Wanibuchi, Gen Murakami, Taro Yamashita, Yoshihiro Minamida, Takanori Fukushima, Allan H. Friedman, Mineko Fujimiya, Kiyohiro Houkin
    NEUROSURGERY 69 1 Suppl Operative 95 - 98 2011年09月 [査読有り][通常論文]
     
    BACKGROUND: The lateral loop formed by the maxillary nerve (V2) and the mandibular nerve (V3) consists of a part of the far lateral triangle of the cavernous sinus. Because this triangle becomes a surgical corridor of the preauricular infratemporal fossa approach and a landmark of the extradural approach for the ganglion-type trigeminal schwannomas, identification of the lateral loop has important implications at the early stage of middle cranial base surgery. We realized that a bony ridge usually existed just lateral to the lateral loop. OBJECTIVE: To nominate midsubtemporal ridge (MSR) as the name for this anatomically unnamed bony ridge and to clarify its features. METHODS: Using 35 cadaver heads, we measured the shape of the MSR on both sides and the distance between the MSR and the adjacent structures. RESULTS: The MSR was recognized in 60 of 70 specimens (85.7%). The bony protrusion was 2.9 +/- 1.1 mm in height, 6.0 +/- 2.1 mm in width, and 9.1 +/- 3.2 mm in length. A single peak with anteroposterior length was common in 47 of 60 specimens (78.3%). The MSR was located at the midpoint of the V2 and V3 in 28 specimens (46.7%) and existed 10.7 +/- 3.6 mm lateral from the line that bound the foramen rotundum and the foramen ovale. CONCLUSION: We demonstrate morphological characteristics of the MSR. These data on the MSR will assist the surgeon in identifying the lateral loop as a surgical landmark during middle cranial base surgery.
  • Shigeru Yamaguchi, Hiroyuki Kobayashi, Takuhito Narita, Koki Kanehira, Shuji Sonezaki, Nobuki Kudo, Yoshinobu Kubota, Shunsuke Terasaka, Kiyohiro Houkin
    ULTRASONICS SONOCHEMISTRY 18 5 1197 - 1204 2011年09月 [査読有り][通常論文]
     
    Sonodynamic therapy is expected to be a novel therapeutic strategy for malignant gliomas. The titanium dioxide (TiO2) nanoparticle, a photosensitizer, can be activated by ultrasound. In this study, by using water-dispersed TiO2 nanoparticles, an in vitro comparison was made between the photodynamic and sonodynamic damages on U251 human glioblastoma cell lines. Water-dispersed TiO2 nanoparticles were constructed by the adsorption of chemically modified polyethylene glycole (PEG) on the TiO2 surface (TiO2/PEG). To evaluate cytotoxicity, U251 monolayer cells were incubated in culture medium including 100 mu g/ml of TiO2/PEG for 3 h and subsequently irradiated by ultraviolet light (5.0 mW/cm(2)) or 1.0 MHz ultrasound (1.0 W/cm(2)). Cell survival was estimated by MTT assay 24 h after irradiation. In the presence of TiO2/PEG, the photodynamic cytotoxic effect was not observed after 20 min of an ultraviolet light exposure, while the sonodynamic cytotoxicity effect was almost proportional to the time of sonication. In addition, photodynamic cytotoxicity of TiO2/PEG was almost completely inhibited by radical scavenger, while suppression of the sonodynamic cytotoxic effect was not significant. Results of various fluorescent stains showed that ultrasound-treated cells lost their viability immediately after irradiation, and cell membranes were especially damaged in comparison with ultraviolet-treated cells. These findings showed a potential application of TiO2/PEG to sonodynamic therapy as a new treatment of malignant gliomas and suggested that the mechanism of TiO2/PEG mediated sonodynamic cytotoxicity differs from that of photodynamic cytotoxicity. (C) 2011 Elsevier B.V. All rights reserved.
  • Masaki Ito, Satoshi Kuroda, Taku Sugiyama, Hideo Shichinohe, Yukari Takeda, Mitsufumi Nishio, Takao Koike, Kiyohiro Houkin
    Translational Stroke Research 2 3 294 - 306 2011年09月 [査読有り][通常論文]
     
    This study was aimed to test the hypothesis that human bone marrow stromal cells (hBMSC) expanded in fetal calf serum (FCS)-free, platelet lysate (PL)-containing medium would retain their capacity of migration, survival, and neural differentiation when transplanted into the infarct brain, using serial in vivo magnetic resonance imaging (MRI). Cell growth kinetic analysis revealed that hBMSC maintain their proliferative activity when cultured either in conventional FCS-containing medium or FCS-free, PL-containing medium. Subsequently, hBMSC were labeled with a superparamagnetic iron oxide agent and were stereotactically transplanted into the ipsilateral striatum of rats at 7 days after permanent middle cerebral artery occlusion. Serial MRI performed over 8 weeks revealed that they retain their migratory capacity towards the cerebral infarct. Moreover, double fluorescence immunohistochemistry also revealed that they preserve their capacity of differentiation into the neural cells in the peri-infarct area. The hBMSC expanded in the FCS-free, PL-containing medium retain their capacity of migration, survival, and differentiation when stereotactically transplanted into the infarct brain. The present findings strongly suggest the clinical utility of PL as a substitute to expand autologous hBMSC for cerebral infarct in the future.
  • Hideo Shichinohe, Satoshi Kuroda, Taku Sugiyama, Masaki Ito, Masahito Kawabori, Mitsufumi Nishio, Yukari Takeda, Takao Koike, Kiyohiro Houkin
    Translational Stroke Research 2 3 307 - 315 2011年09月 [査読有り][通常論文]
     
    The donor cell culture in animal serum-free medium is quite important for the clinical application of cell transplantation therapy. This study was aimed to test the hypothesis that the human bone marrow stromal cells (hBMSC) expanded with fetal calf serum (FCS)-free, platelet lysate (PL)-containing medium retain their biological features favoring central nervous system regeneration. The hBMSC were cultured with 5% PL or 10% FCS. Their phenotypes were analyzed with flow cytometry, and their production of growth factors was quantified with enzyme-linked immunosorbent assay. Their capacity of neural differentiation was verified by immunocytochemistry. There was no significant difference in morphology and cell surface marker between the hBMSC-FCS and hBMSC-PL. Both of them were positive for CD44, CD90, CD105, and CD166 and were negative for CD34, CD45, and CD271. The production of human brain-derived neurotrophic factor, human hepatocyte growth factor, human β-nerve growth factor, and human platelet-derived growth factor-BB did not differ between the two groups, although the hBMSC-PL produced significantly more amount of TGF-β1 than the hBMSC-FCS. There was no significant difference in their in vitro differentiation into the neurons and astrocytes between the two groups. The hBMSC expanded with PL-containing medium retain their biological capacity of neural differentiation and neuroprotection. The PL may be a clinically valuable and safe substitute for FCS in expanding the hBMSC for cell therapy. © 2011 Springer Science+Business Media, LLC.
  • AOYAMA Takeshi, HIDA Kazutoshi, HOUKIN Kiyohiro
    Neurologia medico-chirurgica = 神経外科 51 8 561 - 566 一般社団法人 日本脳神経外科学会 2011年08月15日 [査読無し][通常論文]
     
    Magnetic resonance imaging has increased the incidence of the diagnosis of intramedullary cavernous angioma. Surgical therapy tends not to be recommended for asymptomatic lesions, but symptomatic lesions that bleed recurrently should be treated. The natural course of intramedullary cavernous angioma remains unknown and arguments have been raised against the surgical treatment of symptomatic lesions. We reviewed the clinical features of 13 intramedullary cavernous angiomas in 12 patients surgically treated between 1988 and 2009. The 7 men and 5 women were aged from 14 to 60 years, the preoperative interval ranged from 0 to 161 months, and the mean number of hemorrhages in the 13 lesions was 2.5. Sixteen operations were performed to treat the 13 lesions. The surgical approach depended on the lesion location. The outcome of patients with mild to moderate preoperative symptoms (McCormick grades I-III) was significantly better than that of patients with severe symptoms (McCormick grade IV) (p < 0.05). Symptomatic intramedullary cavernous angioma tends to bleed repeatedly. The lesion should be surgically removed to avoid further deterioration due to recurrent hemorrhages. The shortest path approach should be selected based on preoperative images and complete removal should be attempted. Residual lesion may be masked by surrounding gliosis, so careful postoperative follow up is necessary.
  • 鴨嶋雄大, 寺坂俊介, 浜内祝嗣, 金子貞洋, 村上博基, 寳金清博
    Neurological Surgery 39 8 783 - 788 2011年08月10日 [査読有り][通常論文]
     
    We report a case of cerebrospinal fluid (CSF) leak repair using loose areolar connective tissue insertion into the frontal sinus and pericranial flap covering. A 61-years-old man suffered from skull fracture including frontal sinus fracture in violence inflicted by others. Fifty days later, he presented rhinorrhea and pneumocephalus caused by a bone defect site of the frontal sinus and anterior skull base. We performed CSF leak repair with insertion of pedunculated loose areolar connective tissue into his frontal sinus, covering the leak point using pericranial flap. In general, frontal sinus obliteration has been accomplished with autologous grafts such as fat, muscle, or bone. These avascular grafts carry an increased risk of resorption and infection. The use of loose areolar tissue insertion into the frontal sinus was able to increase stability of the construct and caused no cosmetic troubles in our short follow up period. The combined use of these two autologous materials may be useful for repair of CSF leak from an anterior skull base fracture.
  • KAMOSHIMA Yuuta, SAWAMURA Yutaka, SUGIYAMA Taku, YAMAGUCHI Shigeru, HOUKIN Kiyohiro, KUBOTA Kanako
    Neurologia medico-chirurgica = 神経外科 51 7 527 - 530 一般社団法人 日本脳神経外科学会 2011年07月15日 [査読無し][通常論文]
     
    A 55-year-old woman presented with an extremely rare primary central nervous system mucosa-associated lymphoid tissue (MALT) lymphoma manifesting as seizure and was subsequently diagnosed with dural MALT lymphoma in the cranium. Magnetic resonance imaging revealed a left frontal dural mass with peritumoral edema. Histological examination of the dural mass confirmed MALT lymphoma and revealed infiltration of small cells with irregular nuclear borders and expression of a B cell marker (CD20) but absence of CD5, CD10, CD23, and cyclinD1. Reactive T-cell infiltration was also seen. Subsequently, local irradiation (40 Gy/20 fractions) was performed. Magnetic resonance imaging showed complete remission just after irradiation was completed. There was no evidence of systemic MALT lymphoma. There has been no recurrence for 3 years without additional therapy.
  • Kei Miyata, Takeshi Mikami, Yasufumi Asai, Kazuhisa Mori, Izumi Koyanagi, Nobuhiro Mikuni, Kiyohiro Houkin
    Neurological Surgery 39 7 657 - 662 2011年07月10日 [査読有り][通常論文]
     
    Objective: Intracranial pressure (ICP) is frequently monitored in patients with severe head injuries. In this study of a series of diffuse brain injury patients, we investigated whether there are significant threshold levels that determine the outcome. Method: Data were obtained from 17 patients with severe head injuries (adults aged≥ 15-years-old). The outcome was categorized using the Glasgow Outcome Scale and survival or death. Patients were also grouped according to the Traumatic Coma Data Bank (TCDB) CT classification for diffuse injury: type I (n=0) II(n = 5) III(n=10) IV (n=2). Conclusion and result: The mortality rate was 29% (5 of 17 patients). The average initial ICP within 24 hours (14.08 mmHg) and the peak ICP (26.75 mmHg) were lower in the survivors than that in the patients who died (57.60 mmHg and 91.00 mmHg, respectively p = 0.0006 and 0.0002, respectively). Patients with an initial ICP score> 35 mmHg did not survive, and patients with a peak ICP< 35 mmHg, except one who died of a traumatic brainstem hemorrhage, did survive. Using an X-bar chart, a threshold value for the initial ICP within 24 hours of 27 mmHg and for the peak ICP 46 mmHg appear to be the survival predictors in patients with diffuse brain injury.
  • Takeo Abumiya, Kiyohiro Houkin
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 20 4 319 - 323 2011年07月 [査読有り][通常論文]
     
    Although the platelet aggregation test is the most common method for evaluating response to antiplatelet therapy, little is known about the association of recurrent cerebral infarction with platelet aggregation in the presence of various different antiplatelet drugs. We prospectively evaluated adenosine diphosphate (ADP)- and collagen-induced platelet aggregation and followed the incidence of recurrent infarction in patients categorized into 3 groups based on treatment; aspirin (n = 144), ticlopidine (n = 100), and aspirin + ticlopidine (n = 96). The patients in each treatment group were stratified into quartiles according to platelet aggregation, and the association of recurrent infarction with platelet aggregation was investigated. ADP-induced platelet aggregation values were significantly lower in the ticlopidine group and the aspirin + ticlopidine group compared with the aspirin group (P <.001), and collagen-induced platelet aggregation values were significantly lower in the aspirin group and the aspirin + ticlopidine group compared with the ticlopidine group (P <.001). In the aspirin group, the recurrence rate was somewhat higher in the higher aggregation quartiles than in the lower aggregation quartiles of 2 mu g/mL collagen, the differences were not statistically significantly (P =.243). In the ticlopidine group, the recurrence rate was significantly higher in the lower aggregation quartiles compared with the higher aggregation quartiles of 1 mu mol/L ADP (P =.025). No tendencies were found in the aspirin + ticlopidine group. Although the study is limited by its small sample size, the results suggest a possible difference between aspirin therapy and ticlopidine therapy in the pattern of association of recurrent infarction with platelet aggregation.
  • Shunsuke Terasaka, Hiroyuki Kobayashi, Kiyohiro Houkin
    Neurological Surgery 39 6 553 - 562 2011年06月10日 [査読有り][通常論文]
  • 下田祐介, 黒田敏, 柏崎大奈, 浅野剛, 山下健一郎, 谷口雅彦, 鈴木友己, 嶋村剛, 古川博之, 中山若樹, 寳金清博
    Neurological Surgery 39 6 589 - 594 2011年06月10日 [査読有り][通常論文]
     
    In this report, the authors describe a 35-year-old male whose intracranial arteriovenous malformation (AVM) spontaneously disappeared about 2 years after successful living-donor liver transplantation for alcohol-induced liver cirrhosis. Preoperative screening MRI revealed intracranial arteriovenous malformation (AVM) around the midbrain. Cerebral angiography demonstrated that the AVM was fed by the paramedian mesencephalic arteries and was drained via the vein of Galen. He successfully underwent living-donor liver transplantation, and his postoperative course was uneventful. Follow-up MRI and MRA revealed spontaneous disappearance of the AVM 27 months after surgery. The authors discuss precisely the underlying mechanism of this rare phenomenon, based on thorough literature review.
  • 鴨嶋雄大, 寺坂俊介, 内田和希, 牛越聡, 曹圭龍, 寳金清博
    Neurological Surgery 39 6 601 - 605 2011年06月10日 [査読有り][通常論文]
     
    Ectopic pituitary adenomas are relatively rare tumors. We present a case of ectopic pituitary adenoma occurring in the sphenoid sinus. A 63-year-old woman was referred to our hospital complaining of headache. She had no endocrinological abnormalities. Magnetic resonance imaging showed a tumor in the sphenoid sinus, adjacent to the sellar floor and appearing as a low-signal on T1-weighetd image and a high signal on T2-weighted image. No connection between the normal pituitary gland and tumor was observed. Using an endonasal- transsphenoidal approach assisted with neuro-endoscopy, we performed total removal of the tumor. No connection between the normal pituitary gland and the tumor was found. Histopathological analysis showed a pituitary adenoma. As demonstrated by our case report, differential diagnosis of a tumor occurring in the sphenoid sinus must include consideration of the existence of an ectopic pituitary adenoma.
  • Osamu Honmou, Kiyohiro Houkin, Takuya Matsunaga, Yoshiro Niitsu, Sumio Ishiai, Rie Onodera, Stephen G. Waxman, Jeffery D. Kocsis
    BRAIN 134 Pt 6 1790 - 1807 2011年06月 [査読有り][通常論文]
     
    Transplantation of human mesenchymal stem cells has been shown to reduce infarct size and improve functional outcome in animal models of stroke. Here, we report a study designed to assess feasibility and safety of transplantation of autologous human mesenchymal stem cells expanded in autologous human serum in stroke patients. We report an unblinded study on 12 patients with ischaemic grey matter, white matter and mixed lesions, in contrast to a prior study on autologous mesenchymal stem cells expanded in foetal calf serum that focused on grey matter lesions. Cells cultured in human serum expanded more rapidly than in foetal calf serum, reducing cell preparation time and risk of transmissible disorders such as bovine spongiform encephalomyelitis. Autologous mesenchymal stem cells were delivered intravenously 36-133 days post-stroke. All patients had magnetic resonance angiography to identify vascular lesions, and magnetic resonance imaging prior to cell infusion and at intervals up to 1 year after. Magnetic resonance perfusion-imaging and 3D-tractography were carried out in some patients. Neurological status was scored using the National Institutes of Health Stroke Scale and modified Rankin scores. We did not observe any central nervous system tumours, abnormal cell growths or neurological deterioration, and there was no evidence for venous thromboembolism, systemic malignancy or systemic infection in any of the patients following stem cell infusion. The median daily rate of National Institutes of Health Stroke Scale change was 0.36 during the first week post-infusion, compared with a median daily rate of change of 0.04 from the first day of testing to immediately before infusion. Daily rates of change in National Institutes of Health Stroke Scale scores during longer post-infusion intervals that more closely matched the interval between initial scoring and cell infusion also showed an increase following cell infusion. Mean lesion volume as assessed by magnetic resonance imaging was reduced by > 20% at 1 week post-cell infusion. While we would emphasize that the current study was unblinded, did not assess overall function or relative functional importance of different types of deficits, and does not exclude placebo effects or a contribution of recovery as a result of the natural history of stroke, our observations provide evidence supporting the feasibility and safety of delivery of a relatively large dose of autologous mesenchymal human stem cells, cultured in autologous human serum, into human subjects with stroke and support the need for additional blinded, placebo-controlled studies on autologous mesenchymal human stem cell infusion in stroke.
  • 鴨嶋雄大, 寺坂俊介, 小山明彦, 寳金清博
    Neurological Surgery 39 5 491 - 495 2011年05月10日 [査読有り][通常論文]
     
    We report a case of cranial reconstruction using autologous split calvarial bone combined with calcium phosphate bone cement (CPC). A 19-years-old man suffered from cranium defect and rhinorrhea originating from frontal skull base fracture in a traffic accident. After CSF hydration treatment had finished, continuously we performed cranial reconstruction with autologous split calvarial bone so that the patient could return to work at an early stage. The use of autologous split calvarial bone with CPC was able to increase stability of the construct and provide excellent cosmetic result in our short follow up period. The combination use of these two materials may be useful for cranial reconstruction in patients with cranium defect.
  • 笹森徹, 飛騨一利, 浅野剛, 青山剛, 山内朋裕, 岩崎素之, 矢野俊介, 岩崎喜信, 寳金清博
    Neurological Surgery 39 5 505 - 511 2011年05月10日 [査読有り][通常論文]
     
    Spinal dural arteriovenous fistula (SDAVF) in the sacral region is relatively rare and remains difficult to diagnose because of the uncommon origin of its feeder. It also has higher incidence of recurrence than usual thoraco-lumbar lesion and needs subsequent treatment. We reviewed 51 cases of SDAVF over the past 10 years. Especially in patients with sacral lesion, clinical features and the findings on spinal angiography were analyzed. Four patients (7.8%) had SDAVF in the sacral region. In all cases, SDAVF were supplied by the lateral sacral artery. Multiple feeders were observed in 3 (75%) out of 4 patients and 2 patients (50%) had multiple fistulas. Endovascular embolizations were performed in all patients, and neurological symptoms were improved in two patients (50%) and the other two were stabilized (50%). There was no recurrence during a follow-up period of 3 months to 8 years. We should keep in mind that SDAVF in the sacral region can have multiple shunts and feeders derived from the lateral sacral artery.
  • 鴨嶋雄大, 澤村豊, 斉藤伸治, 川堀真人, 寳金清博, 久保田佳奈子
    Neurological Surgery 39 5 479 - 83 2011年05月 [査読有り][通常論文]
     
    A 1-year-old female infant presented with congenital giant, hairy and pigmented nevi. MRI scan as screening test revealed a cerebellar tumor. A diagnosis of provisional neurocutaneous melanosis was made on the basis of the patient's MRI and physical findings. At her 6 years of age, MRI revealed the tumor grown up to 3 cm diameter in 5 years. The cerebellar tumor was removed partially using the occipital transtentorial approach for tissue diagnosis. The color of the cerebellar tumor was whitish and contained neither benign nor malignant melanocyte. Pathological examination revealed diffuse astrocytoma. Finally residual cerebellar tumor was totally removed at a second surgical resection. To our knowledge, this is the first patient to be reported with astrocytoma complicated giant skin nevus except neurocutaneous syndrome cases.
  • 笹森 徹, 飛騨 一利, 浅野 剛, 青山 剛, 山内 朋裕, 岩崎 素之, 矢野 俊介, 岩崎 喜信, 寳金 清博
    Neurological Surgery 39 5 505 - 511 (株)医学書院 2011年05月 
    過去10年間に脊髄硬膜動静脈瘻(SDAVF)の診断で治療した51例中仙骨部病変の4例(全例男性、57〜76歳)を対象に、臨床経過、脊髄血管造影検査所見について検討した。初発症状は両下肢しびれ2例、歩行障害2例で、いずれも緩徐進行性であった。再発で紹介の1例を除き発症から平均20.7ヵ月でSDAVFと診断した。脊髄血管造影検査では、仙骨部病変はS2レベル:3例、S1レベル:1例、全例で外側仙骨動脈からfeederを認め、拡張したfilm terminale上のdraining veinへの頭側方向に環流を認めた。また、3例で両側の外側仙骨動脈から複数のfeederを、2例に複数のfistulaを認めた。全例でN-butyl cyanoacrylateを用いた血管内塞栓術を行い、3ヵ月〜8年のフォローアップ期間で2例に神経症状の改善、2例に神経症状の進行抑制を認め、全例で再発は認めていない。
  • 齋藤久泰, 飛騨一利, 浅野剛, 矢野俊介, 青山剛, 岩崎喜信, 寳金清博
    Neurological Surgery 39 4 375 - 380 2011年04月10日 [査読有り][通常論文]
     
    Penmedullary arteriovenous fistula (AVF) is usually located on the surface of the spinal cord and is fed by the anterior spinal artery and/or the posterior spinal artery. We report a rare case of Conus penmedullary AVF with multiple shunt points including the cauda equina. A 68-year-old man presented with leg pain when walking long distances. Magnetic resonance imaging at the thoracic and lumbar level revealed multiple signal voids with marked cord signal change. Angiography showed the penmedullary AVF fed by the anterior spinal artery from the right T9 intercostal artery at the level of the conus medullaris and the fistula fed by the left lateral sacral artery from the left internal iliac artery at the level of the cauda equina. In the first surgery, we performed surgical interruption of feeding arteries from the filum terminale and coagulated AV shunt of the conus medullaris. However residual perimedullary AVF was found at the conus medullaris in the postoperative angiography. Secondary surgery was carried out to treat residual AVF. Follow-up angiography showed complete disappearance of all AVFs. Postoperatively, the patient's symptoms were improved. Because the Conus perimedullary AVF has the characteristics of multiple feeding arteies. multiple shunt points, and complex venous drainage, it must be kept in mind that other fistula could exist in the cauda equina or filum terminale.
  • 中山若樹, 石川達哉, 宝金清博, 黒田敏, 上山博康
    脳神経外科ジャーナル 20 Supplement 46  2011年04月10日 [査読無し][通常論文]
  • 鴨嶋雄大, 澤村豊, 川嶋邦弘, 長内俊也, 柏崎大奈, 山内朋裕, 寳金清博
    Neurological Surgery 39 4 351 - 8 2011年04月 [査読有り][通常論文]
     
    We present a modified method for reconstruction of calvarial bone defects for patients with a history of infectious complications. Three patients who had experienced implanted bone infections underwent reconstruction of calvarial bone defect. For reconstruction of the calvarial bone defects, autologous split calvarial bone grafts were used to cover the calvarial bone defect. The full or half layered fronto-parietal bone used as implants were fixed with titanium mini-plates for primary bone defect site, while the new bone defect site caused by getting autologous bone graft were covered with titanium mesh plates assisted by residual half layered calvarias. The average follow-up span of patients was 64 months. Evaluated clinical and radiologic results are stable, showing no measurable side effects. Split calvarial bone graft in combination with titanium mesh plates is recommended in patients with a history of infection or high risk of infection.
  • Tomohiro Murakami, Izumi Koyanagi, Takahisa Kaneko, Satoshi Iihoshi, Kiyohiro Houkin
    NEUROSURGERY 68 3 E241 - E245 2011年03月 [査読有り][通常論文]
     
    BACKGROUND AND IMPORTANCE: In surgery of spinal vascular lesions such as spinal arteriovenous fistula or vascular tumors, assessment of feeding arteries and draining veins is important. Intraoperative digital subtraction angiography is useful but is invasive and sometimes technically demanding. Near-infrared indocyanine green (ICG) video-angiography is less invasive and has been reported as an intraoperative diagnosis of arterial patency during clipping surgery of cerebral aneurysms or bypass surgeries. We present our experience with intraoperative ICG videoangiography in 3 cases of spinal vascular lesions. CLINICAL PRESENTATION: Two patients had spinal arteriovenous fistula (perimedullary, n = 1; dural, n = 1), and 1 patient had spinal cord hemangioblastoma at the thoracic or thoracolumbar level. The surgical microscope was an OPMI Pentero (Carl Zeiss, Oberkochen, Germany). After laminectomy and opening of the dura, ICG (5 mg) was injected intravenously. The ICG angiography clearly demonstrated feeding and draining vessels. The ICG findings greatly helped successful interruption of arteriovenous fistula and total removal of the tumor. CONCLUSION: Intraoperative ICG videoangiography for spinal vascular lesions was useful by providing information on vascular dynamics directly. However, the diagnostic area is limited to the field of the surgical microscope. Although intraoperative digital subtraction angiography is still needed in cases of complex spinal vascular lesions, ICG videoangiography will be an important diagnostic modality in the field of spinal vascular surgeries.
  • Tomohiro Murakami, Izumi Koyanagi, Takahisa Kaneko, Satoshi Iihoshi, Kiyohiro Houkin
    NEUROSURGERY 68 2011年03月 [査読有り][通常論文]
     
    BACKGROUND AND IMPORTANCE: In surgery of spinal vascular lesions such as spinal arteriovenous fistula or vascular tumors, assessment of feeding arteries and draining veins is important. Intraoperative digital subtraction angiography is useful but is invasive and sometimes technically demanding. Near-infrared indocyanine green (ICG) videoangiography is less invasive and has been reported as an intraoperative diagnosis of arterial patency during clipping surgery of cerebral aneurysms or bypass surgeries. We present our experience with intraoperative ICG videoangiography in 3 cases of spinal vascular lesions. CLINICAL PRESENTATION: Two patients had spinal arteriovenous fistula (perimedullary, n = 1; dural, n = 1), and 1 patient had spinal cord hemangioblastoma at the thoracic or thoracolumbar level. The surgical microscope was an OPMI Pentero (Carl Zeiss, Oberkochen, Germany). After laminectomy and opening of the dura, ICG (5 mg) was injected intravenously. The ICG angiography clearly demonstrated feeding and draining vessels. The ICG findings greatly helped successful interruption of arteriovenous fistula and total removal of the tumor. CONCLUSION: Intraoperative ICG videoangiography for spinal vascular lesions was useful by providing information on vascular dynamics directly. However, the diagnostic area is limited to the field of the surgical microscope. Although intraoperative digital subtraction angiography is still needed in cases of complex spinal vascular lesions, ICG videoangiography will be an important diagnostic modality in the field of spinal vascular surgeries.
  • Miyamoto Michiyuki, Kuroda Satoshi, Nakayama Naoki, Hokari Masaaki, Yasuda Hiroshi, Saito Hisatoshi, Houkin Kiyohiro
    STROKE 42 3 E172  2011年03月 [査読有り][通常論文]
  • Kuroda Satoshi, Maruichi Katsuhiko, Nakayama Naoki, Houkin Kiyohiro, Hirata Kenji, Shiga Tohru, Tamaki Nagara
    STROKE 42 3 E183  2011年03月 [査読有り][通常論文]
  • 杉山拓, 黒田敏, 中山若樹, 寳金清博
    Neurological Surgery 39 2 155 - 161 2011年02月10日 [査読有り][通常論文]
     
    Fungal infection is an increasing problem in patients treated with intensive chemotherapy or immunosuppressive therapy. Of these, invasive aspergillosis or mucormycosis on rare occasions, infiltrate the internal carotid artery (ICA) by contiguous spread from an infected paranasal sinus. Here, we report three cases of ICA infiltration secondary to the fungal infections of the paranasal sinus. All cases developed orbital apex syndrome when the lesion spread to the orbital apex or cavernous sinus, and aggressive progression led to ICA invasion causing life-threatening cerebral infarction or hemorrhage. In this case report, we describe the aggressive clinical course of the fungal ICA invasion from the paranasal sinus, and then discuss the clinical feature, diagnostic methods, and current treatment of intracranial fungal infection.
  • Haruto Uchino, Takeshi Asano, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
    Neurological Surgery 39 1 59 - 63 2011年01月 [査読有り][通常論文]
     
    The authors present the case of a 61-year-old male with a de novo fusiform vertebral artery aneurysm, probably due to non-traumatic dissection. He underwent flow diversion therapy, using a double overlapping technique, because the origin of the contralateral vertebral artery was stenotic. Placement of two stents resulted in marked reduction of blood flow in the aneurysm. Postoperative course was uneventful. Follow-up 3D-CT angiography revealed a patent blood flow in the stent and a gradual progression of intra-aneurysmal thrombosis. Flow diversion therapy can potentially obliterate the complicated cerebral aneurysm with the preservation of blood flow in the parent artery, and would be one of the important therapeutic options in patients with complicated aneurysms in which conventional strategies such as neck clipping, endovascular coiling and parent artery occlusion are not feasible or contraindicated.
  • Yusuke Shimoda, Kohsuke Kudo, Satoshi Kuroda, Yuri Zaitsu, Nobuyuki Fujima, Satoshi Terae, Makoto Sasaki, Kiyohiro Houkin
    MAGNETIC RESONANCE IN MEDICAL SCIENCES 10 1 49 - 52 2011年 [査読有り][通常論文]
     
    We performed magnetic resonance angiography (MRA) and susceptibility-weighted imaging (SWI) to observe cerebral changes during a migraine attack in a 28-year-old man. MRA revealed regional arterial spasm, and findings of SWI were consistent with decreased blood flow and/or venous dilatation. Because these noninvasive techniques can be performed repeatedly at short intervals, they can be useful for evaluating hemodynamic changes during and after migraine attacks.
  • Haruto Uchino, Shunsuke Terasaka, Shigeru Yamaguchi, Hiroyuki Kobayashi, Kagari Kawai, Kanako Kubota, Shinji Ooe, Kiyohiro Houkin
    Brain and Nerve 63 1 79 - 83 2011年01月01日 [査読有り][通常論文]
     
    Nontuberculous mycobacteria rarely infect the central nervous system. Recently, Mycobacterium genavense, nontuberculous mycobacterium (NTM), has been identified as a significant pathogen in patients. In this report, we describe multiple intracranial lesions caused by M. genavense in an immunocompromised host. A 50-year-old man presented with dysarthria, aphasia, and right hemiparesis. He had a primary immunodeficiency and a M. genavense infection in the thoracic and abdominal lymph nodes. Magnetic resonance imaging revealed multiple intracranial masses in the subcortical regions with extensive perifocal edema. Laboratory investigations and cultures of cerebrospinal fluid provided no evidence of disseminated infection. We obtained a biopsy sample via a small craniotomy using neuronavigation, and NTM infection was confirmed on analysis of the specimen. He was treated with antimycobacterial agents, and the clinical symptoms and radiological findings improved. Although a surgical procedure bears the potential risk of infection, especially in an immunocompromised patient, a brain biopsy was necessary for definitive diagnosis in this case and it aided in the administration of appropriate treatment.
  • 大里俊明, 大里俊明, 齊藤正樹, 齊藤正樹, 齊藤正樹, 奈良理, 奈良理, 宮田圭, 宮田圭, 浅井康文, 浅井康文, 中川原譲二, 中川原譲二, 寳金清博, 寳金清博
    日本脳神経外科救急学会プログラム・抄録集 16th (CD-ROM) 2011年
  • 寺坂俊介, 小林浩之, 寳金清博
    Neurological Surgery 39 6 2011年
  • 栗栖宏多, 黒田敏, 中山若樹, 内野晴登, 中村俊孝, 寶金清博
    脳循環代謝 23 1 157  2011年 [査読無し][通常論文]
  • 黒田 敏, 中山 若樹, 平田 健司, 志賀 哲, 玉木 長良, 宝金 清博
    脳循環代謝 22 1 78 - 78 (一社)日本脳循環代謝学会 2010年11月 [査読無し][通常論文]
  • 川堀 真人, 黒田 敏, 中山 若樹, 宝金 清博
    脳循環代謝 22 1 80 - 80 (一社)日本脳循環代謝学会 2010年11月 [査読無し][通常論文]
  • 金子高久, 小柳泉, 村上友宏, 寳金清博
    Neurological Surgery 38 9 839 - 843 2010年09月10日 [査読有り][通常論文]
     
    We report a case of a 61-year-old man with ankylosing spondylitis who showed cervical spine fracture. The patient had fallen down on the floor and presented with severe neck pain. He was treated conservatively with a hard neck collar in an emergency hospital because of C7 body fracture without dislocation. However, the follow-up radiographs demonstrated a progressive C6-7 anterior dislocation. He was referred to our hospital 6 weeks after the trauma. The 3D-CT reconstruction imaging revealed that the fracture extended from the C7 vertebral body to the C6 lamina via the bilateral C6/7 facet joints. The patient underwent C2-Th3 posterior fixation using pedicle and lateral mass screw techniques. The postoperative course was uneventful. He was discharged without any complication at 1 month postoperatively. The radiograph 3 months after surgery showed good bone fusion. Spine fracture with ankylosing spondylitis usually shows significant instability because of the long lever-arm of the fused vertebrae at the fracture level. Solid spinal fusions such as long posterior fusion or anterior-posterior simultaneous fusion are needed in such cases.
  • Kengo Suzuki, Hiroyuki Momota, Akiko Tonooka, Hiroko Noguchi, Kouhei Yamamoto, Masahiko Wanibuchi, Yoshihiro Minamida, Tadashi Hasegawa, Kiyohiro Houkin
    JOURNAL OF NEURO-ONCOLOGY 99 1 147 - 153 2010年08月 [査読有り][通常論文]
     
    The simultaneous occurrence of multiple primary intracranial tumors has been reported previously. However, most of these tumors arise after cranial radiotherapy or in association with familial tumor syndromes. Double tumors of different histologies that are unrelated to radiotherapy or genetic disorders are very rare. We present a case of two primary intracranial tumors occurring simultaneously at adjacent sites. Preoperative gadolinium-enhanced magnetic resonance imaging of these tumors revealed a single continuous lesion. Postoperative histological examination revealed the presence of two distinct tumors, meningioma and glioblastoma multiforme. To elucidate the mechanism of synchronous tumor formation, we performed immunohistochemical analysis of the proteins involved in the receptor tyrosine kinase, Wnt, and Notch signaling pathways. These analyses showed that platelet-derived growth factor (PDGF) receptors-alpha and beta were overexpressed in both tumors, thereby indicating the oncogenic effects of activated signaling of these receptors. The PDGF-mediated paracrine system may induce one tumor from another.
  • Toshiya Sugino, Mitsuhisa Maruyama, Masaya Tanno, Atsushi Kuno, Kiyohiro Houkin, Yoshiyuki Horio
    FEBS letters 584 13 2821 - 6 2010年07月02日 [査読有り][通常論文]
     
    SIRT1, a NAD(+)-dependent protein deacetylase, is known to have neural functions. However, despite its cytoplasmic expression in some neural cells, its cytoplasmic function, if any, is unknown. Here we found that PC12 (pheochromocytoma) cells expressed SIRT1 in the cytoplasm. Nerve growth factor (NGF)-induced neurite outgrowth of these cells was promoted by activators of SIRT1, while inhibitors of SIRT1 or SIRT1-siRNA significantly inhibited it. The overexpression of a mutant SIRT1 that localised to the cytoplasm but not the nucleus enhanced the NGF-dependent neurite outgrowth, and a cytoplasmic dominant-negative SIRT1 suppressed it. Thus, cytoplasmic SIRT1 increases the NGF-induced neurite outgrowth of PC12 cells.
  • Izumi Koyanagi, Kiyohiro Houkin
    NEUROSURGICAL REVIEW 33 3 271 - 284 2010年07月 [査読有り][通常論文]
     
    The exact pathogenesis of syringomyelia associated with Chiari type 1 malformation is unknown, although a number of authors have reported their theories of syrinx formation. The purpose of this review is to understand evidences based on the known theories and to create a new hypothesis of the pathogenesis. We critically review the literatures on clinicopathological, radiological, and clinical features of this disorder. The previously proposed theories mainly focused on the driven mechanisms of the cerebrospinal fluid (CSF) into the spinal cord. They did not fully explain radiological features or effects of surgical treatment such as shunting procedures. Common findings of the syrinx in clinicopathological studies were the communication with the central canal and extracanalicular extension to the posterior gray matter. Most of the magnetic resonance imaging studies demonstrated blockade and alternated CSF dynamics at the foramen magnum, but failed to show direct communication of the syrinx with the CSF spaces. Pressure studies revealed almost identical intrasyrinx pressure to the subarachnoid space and decreased compliance of the spinal CSF space. Recent imaging studies suggest that the extracellular fluid accumulation may play an important role. The review of evidences promotes a new hypothesis of syrinx formation. Decreased absorption mechanisms of the extracellular fluid may underlie the pathogenesis of syringomyelia. Reduced compliance of the posterior spinal veins associated with the decreased compliance of the spinal subarachnoid space will result in disturbed absorption of the extracellular fluid through the intramedullary venous channels and formation of syringomyelia.
  • Takeo Baba, Izumi Koyanagi, Kazuhisa Yoshifuji, Tomohiro Murakami, Kiyohiro Houkin
    ACTA NEUROCHIRURGICA 152 7 1245 - 1249 2010年07月 [査読有り][通常論文]
     
    Symptomatic spinal arachnoid cyst is a rare disease and the pathophysiology causing spinal cord symptoms has not been well clarified. The authors report three symptomatic cases of spinal arachnoid cyst at the thoracic level. These patients, aged from 70 to 73 years, showed progressive gait disturbance for a few months before admission. Phase-contrast cine magnetic resonance imaging demonstrated significant compression at the rostral side of the cyst during the diastolic phase of the cardiac cycle. Intraoperative ultrasonography demonstrated that the maximum expansion of the cyst and compression of the dorsal spinal cord occurred when the cerebrospinal fluid moved rostrally during diastole. All patients showed good improvement of their symptoms after surgical removal of the arachnoid cyst. This report proposes the pathophysiology that the pulsatile enlargement of the arachnoid cyst during diastolic cardiac phase can be an important factor for deterioration of spinal cord symptoms.
  • Misuzu Osaka, Osamu Honmou, Tomohiro Murakami, Tadashi Nonaka, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
    BRAIN RESEARCH 1343 226 - 235 2010年07月 [査読有り][通常論文]
     
    Transplantation of mesenchymal stem cells (MSCs) derived from bone marrow has been shown to improve functional outcome in spinal cord injury (SCI). Systemic delivery of MSCs results in therapeutic benefits in a number of experimental central nervous system disorders. In the present study we intravenously administered rat MSCs derived from bone marrow at various time points after induction of a severe contusive SCI in rat to study their therapeutic effects. MSCs were systemically delivered at varied time points (6 h to 28 days after SCI). The spinal cords were examined histologically 6 weeks after SCI. Stereological quantification was performed on the spinal cords to determine donor cell (MSCs transduced with the LacZ gene) density in the lesions. Light microscopic examination revealed that cavitation in the contused spinal cords was less in the MSC-treated rats. A limited number of cells derived from MSCs (LacZ(+)) in the injury site expressed neural or glial markers. Functional outcome measurements using the Basso-Beattie-Bresnehan (BBB) score were performed periodically up to 6 weeks post-SCI. Locomotor recovery improvement was greater in the MSC-treated groups than in sham controls with greatest improvement in the earlier post-contusion infusion times. The availability of autologous MSCs in large number and the potential for systemically delivering cells to target lesion areas without neurosurgical intervention suggests the potential utility of intravenous cell delivery as a prospective therapeutic approach in acute and subacute SCI. (C) 2010 Elsevier B.V. All rights reserved.
  • Toshiya Sugino, Mitsuhisa Maruyama, Masaya Tanno, Atsushi Kuno, Kiyohiro Houkin, Yoshiyuki Horio
    FEBS LETTERS 584 13 2821 - 2826 2010年07月 [査読有り][通常論文]
     
    SIRT1, a NAD(+)-dependent protein deacetylase, is known to have neural functions. However, despite its cytoplasmic expression in some neural cells, its cytoplasmic function, if any, is unknown. Here we found that PC12 (pheochromocytoma) cells expressed SIRT1 in the cytoplasm. Nerve growth factor (NGF)-induced neurite outgrowth of these cells was promoted by activators of SIRT1, while inhibitors of SIRT1 or SIRT1-siRNA significantly inhibited it. The overexpression of a mutant SIRT1 that localised to the cytoplasm but not the nucleus enhanced the NGF-dependent neurite outgrowth, and a cytoplasmic dominant-negative SIRT1 suppressed it. Thus, cytoplasmic SIRT1 increases the NGF-induced neurite outgrowth of PC12 cells. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
  • Satoshi Kuroda, Kiyohiro Houkin, Tatsuya Ishikawa, Naoki Nakayama, Yoshinobu Iwasaki
    Neurosurgery 66 6 1093 - 1101 2010年06月 [査読有り][通常論文]
     
    Objective: We reviewed our 11-year experience with a novel bypass procedure, superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis and encephalo-duro-myo-arterio-pericranio-synangiosis (EDMAPS), for moyamoya disease regarding cerebral hemodynamics and long-term outcome. Methods: This prospective study included 75 patients with moyamoya disease, including 28 children and 47 adults. We performed STA-MCA anastomosis and EDMAPS on 123 hemispheres of 75 patients. In addition to conventional STA-MCA anastomosis and indirect bypass for the MCA territory, the medial frontal lobe was revascularized using the frontal pericranial flap through medial frontal craniotomy. Surgical results were analyzed with magnetic resonance imaging, cerebral angiography, and single-photon emission computed tomography/positron emission tomography. Results: Overall incidences of mortality and morbidity were 0% and 5.7%, respectively. The annual risk of cerebrovascular events during the follow-up periods was very low: 0% in pediatric patients and 0.4% in adults over approximately 67 months. Postoperative cerebral angiography showed that the pericranial flap functioned well as donor tissue for indirect bypass, especially in pediatric patients. Follow-up single-photon emission computed tomography/positron emission tomography studies revealed that cerebral blood flow and its reactivity to acetazolamide markedly improved in both the MCA and anterior cerebral artery territories. Conclusion: These findings strongly suggest that STA-MCA anastomosis and EDMAPS using a frontal pericranial flap is a safe and effective surgical procedure to further improve the long-term prognosis in moyamoya disease by improving cerebral hemodynamics in both the MCA and anterior cerebral artery territories. Copyright © 2010 by the Congress of Neurological Surgeons.
  • Osamu Honmou, Kiyohiro Houkin
    Neurological Surgery 38 6 515 - 520 2010年06月 [査読有り][通常論文]
  • 飯星智史, 野中雅, 宮田圭, 寳金清博
    Neurological Surgery 38 6 523 - 530 2010年06月 [査読有り][通常論文]
     
    Object: The anterior choroidal artery (AchoA) is a very important artery for neurosurgeons and neuroendovascular surgeons. In fact the AchoA not only supplies the ventricles but also has important branches to the diencephalon, mesencephalon and cerebrum and also supplies motor and visual pathways. Review of the cerebrovascular embryology explains this vanability in the anatomy of the developing AchoA and posterior communicating artery (PcomA)-posterior cerebral artery (PCA) distributions. Case 1: A 47-year-old woman, with an unruptured left internal carotid artery (ICA) aneurysm. For the cerebral arterial aneurysm approximately 7 mm in size, we performed coil embolization with an assist balloon under general anaesthesia. Case 2: A 63-year-old woman, with an unruptured right ICA aneurysm. Endeavouring to preserve the branch vessels, we undertook a craniotomy neck clipping operation. Discussion and Conclusion: It is thought that, usually. AchoA has its origin at the supraclinoid ICA distal to the PcomA. Our analysis of these two cases may be helpful for a better understanding of the vascular relations and anatomy of the AchoA and PcomA-PCA distributions. We emphasize the importance of recognizing such anomalies in planning surgical and interventional treatment.
  • Katsuya Komatsu, Osamu Honmou, Junpei Suzuki, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
    BRAIN RESEARCH 1334 84 - 92 2010年06月 [査読有り][通常論文]
     
    Intravenous transplantation of mesenchymal stem cells (MSCs) prepared from bone marrow ameliorates functional deficits in rat cerebral infarction models. Although several hypotheses to account for these therapeutic effects have been suggested, angiogenesis is thought to be largely responsible. MSCs were intravenously infused into rats in the relatively later time points after permanent middle cerebral artery occlusion (MCAO) to determine if they could contribute to neovascularization and functional recovery. Although MRI analysis revealed that only rats receiving MSCs 7 days after MCAO exhibited decreased ischemic lesion volume than the control group, all MSCs treated rats receiving MSCs up to 1 month after MCAO had greater angiogenesis near the border of the ischemic lesions and showed greater functional recovery in the treadmill stress test than did control rats. Thus, these results suggest that the time window of MSC therapy is at least over 1 month after cerebral infarction in the rat permanent MCAO model, and systemic delivery of MSCs in the later phase after cerebral ischemia may have beneficial effect through an angiogenic mechanism. (c) 2010 Elsevier B.V. All rights reserved.
  • Masahiko Wanibuchi, Masafumi Ohtaki, Takanori Fukushima, Allan H. Friedman, Kiyohiro Houkin
    ACTA NEUROCHIRURGICA 152 6 1055 - 1060 2010年06月 [査読有り][通常論文]
     
    Practicing skull base approaches on cadavers affords the surgeon a chance to learn complex anatomical relationships and to practice surgical skills. However, there are ethical or legal problems in obtaining cadaver material in some countries. In addition, there is always risk of transmitting infections with cadaveric material. In order to get around these problems, we created a whole skull model which reproduces the detailed anatomy within the skull base using a selective laser sintering (SLS) technique. The first author's head was scanned using multidetector-row computed tomography. The data were reconstructed and converted into the standard triangulation language file system. Powdered material comprised of polyamide nylon and glass beads was laser-sintered in accord with the data derived from the head CT. The model was dissected under a surgical microscope using a high-speed drill, suction, and other surgical instruments. The appearance of both inner and outer cranial surfaces, including sutures, foramens, fissures, and protrusions, were clearly demonstrated. The artificial mastoid did not melt from the heat of the drill when a mastoidectomy was performed. The anatomical structures inside the mastoid and of paranasal sinuses were accurately reproduced in the model. The model created using SLS should be very useful for the teaching skull base approaches avoiding the ethical, legal, and infection problems inherent in cadavers.
  • Satoshi Kuroda, Kiyohiro Houkin, Tatsuya Ishikawa, Naoki Nakayama, Yoshinobu Iwasaki
    NEUROSURGERY 66 6 1093 - 1101 2010年06月 [査読有り][通常論文]
     
    OBJECTIVE: We reviewed our 11-year experience with a novel bypass procedure, superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis and encephalo-duro-myo-arterio-pericranio-synangiosis (EDMAPS), for moyamoya disease regarding cerebral hemodynamics and long-term outcome. METHODS: This prospective study included 75 patients with moyamoya disease, including 28 children and 47 adults. We performed STA-MCA anastomosis and EDMAPS on 123 hemispheres of 75 patients. In addition to conventional STA-MCA anastomosis and indirect bypass for the MCA territory, the medial frontal lobe was revascularized using the frontal pericranial flap through medial frontal craniotomy. Surgical results were analyzed with magnetic resonance imaging, cerebral angiography, and single-photon emission computed tomography/positron emission tomography. RESULTS: Overall incidences of mortality and morbidity were 0% and 5.7%, respectively. The annual risk of cerebrovascular events during the follow-up periods was very low: 0% in pediatric patients and 0.4% in adults over approximately 67 months. Postoperative cerebral angiography showed that the pericranial flap functioned well as donor tissue for indirect bypass, especially in pediatric patients. Follow-up single-photon emission computed tomography/positron emission tomography studies revealed that cerebral blood flow and its reactivity to acetazolamide markedly improved in both the MCA and anterior cerebral artery territories. CONCLUSION: These findings strongly suggest that STA-MCA anastomosis and EDMAPS using a frontal pericranial flap is a safe and effective surgical procedure to further improve the long-term prognosis in moyamoya disease by improving cerebral hemodynamics in both the MCA and anterior cerebral artery territories.
  • 黒田 敏, 笹森 徹, 川堀 真人, 中山 若樹, 宝金 清博, 石川 達哉
    小児の脳神経 35 2 183 - 183 (一社)日本小児神経外科学会 2010年04月 [査読無し][通常論文]
  • Masako Nozaki, Masanobu Yoshikawa, Kunihiko Ishitani, Hiroyuki Kobayashi, Kiyohiro Houkin, Kohzoh Imai, Yoichiro Ito, Takamura Muraki
    Keio Journal of Medicine 59 1 10 - 18 2010年03月 [査読有り][通常論文]
     
    We explored the possibility of the cysteinyl leukotriene receptor antagonists, pranlukast and montelukast, preventing tumor cell migration through both cerebral and peripheral capillaries. To study tumor cell migration through brain capillaries, male Fisher rats were cannulated via the cisterna magna under pentobarbital anesthesia. RCN9 cells labeled with a fluorescent marker PKH67 were intravenously administered following arachidonic acid administration into the subarachnoid space, and specimens of the central nervous system were collected every 30 min for 8 h. Arachidonic acid increased the fluid volume with elevated white blood cell and RCN9 cell counts. When given 2 h before arachidonic acid administration, pranlukast, but not montelukast, reduced the fluid volume and inhibited white blood cell and RCN9 cell extravasation through the brain capillary. In addition, a Lewis lung carcinoma metastasis model in mice was used to study the inhibitory effect of pranlukast and montelukast against cancer cell extravasation through general capillaries. When mice were given food containing either pranlukast or montelukast, immediately after paw amputation, tumor metastasis was prevented by both drugs, and their survival was prolonged. These results show that pranlukast can inhibit tumor cell migration through both the brain and peripheral capillaries, whereas montelukast inhibits tumor cell migration only in the peripheral capillaries.
  • Yukinori Akiyama, Kiyohiro Houkin, Kazuhiko Nozaki, Nobuo Hashimoto
    CEREBROVASCULAR DISEASES 30 5 491 - 499 2010年 [査読有り][通常論文]
     
    Background: Decision-making during the management of unruptured cerebral aneurysms is a delicate process for both neurosurgeons and patients. Guidelines are evidence-based references that can aid in making decisions. However, neurosurgeons do not always follow guidelines in clinical practice. The purpose of this study is to verify the hypothesis that there is substantial dissociation between treatment guidelines and practical decision-making due to a bias in treatment selection for unruptured cerebral aneurysms. This bias is dependent upon clinician-driven factors such as experience and specialty, and patient-driven factors such as patient preference. Methods: This study was performed using internet questionnaires. A total of 282 randomly selected, qualified Japanese neurosurgeons (out of approx. 6,000 registered neurosurgeons), including 45 endovascular specialists, participated in this study. Radiological and demographic data from 88 cases of unruptured cerebral aneurysm were opened on the Web. Participating neurosurgeons decided on the treatment for each case (clipping, coiling or observation). Results: Variations in treatment selection were not significant between neurosurgeons and endovascular specialists, except for aneurysms such as anterior choroidal artery aneurysm. However, contrary to the guidelines, aneurysms larger than 10 mm tended to be treated conservatively because the risk of treatment is high, while aneurysms smaller than 5 mm in diameter were often selected for intervention (clipping or coiling). Conclusions: This study revealed that in real-world clinical practice, physicians are not always faithful to the current guidelines. In making practical treatment decisions for unruptured cerebral aneurysms, the patient's will and the recognition of unavoidable, treatment-related risks seriously influence neurosurgeons' decisions. Copyright (C) 2010 S. Karger AG, Basel
  • Wei Zheng, Osamu Honmou, Kei Miyata, Kuniaki Harada, Junpei Suzuki, He Liu, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
    BRAIN RESEARCH 1310 8 - 16 2010年01月 [査読有り][通常論文]
     
    Although intravenous delivery of mesenchymal stem cells (MSCs) prepared from adult bone marrow reduces infarction size and ameliorates functional deficits in rat middle cerebral artery occlusion models, there are few reports of MSC treatment in global cerebral ischemia. We utilized a global cerebral ischemia model induced by arresting the heart with a combination of hypovolemia and intracardiac injections of a cold potassium chloride solution in order to study the potential therapeutic benefits of human mesenchymal. stem cells (hMSCs) on global cerebral ischemia. hMSCs were intravenously injected into the rats 3 h after resuscitation from cardiac arrest. The effects on structural and functional outcome of hMSC were assessed at 5 h and 1, 3, and 7 days using magnetic resonance spectroscopy (MRS), histology, and cognitive functional analysis. intravenous delivery of hMSCs reduced the Lac/Cr ratios, nuclear DNA fragmentation, neuronal loss, and elicited functional improvement compared with the control sham group. Enzyme-linked immunosorbent assay (ELISA) of the hippocampus revealed an increase in BDNF in hMSC-treated group. These data suggest that intravenous delivery of hMSC may have a therapeutic effect in global cerebral ischemia. (C) 2009 Elsevier B.V. All rights reserved.
  • Toshiya Sugino, Masafumi Ohtaki, Masahiko Wanibuchi, Sonen Kin, Kiyohiro Houkin
    Neurologia medico-chirurgica 50 4 306 - 9 2010年 [査読有り][通常論文]
     
    A 68-year-old woman presented with severe headache 9 days after undergoing successful clipping of a right middle cerebral artery aneurysm. Postoperative imaging revealed increased perfusion and diffuse edema in the right frontotemporal cortex. A 57-year-old woman exhibited perseveration soon after undergoing successful clipping of an anterior communicating artery aneurysm. Postoperative imaging studies revealed increased perfusion and diffuse edema in the left frontal and insular cortex. The symptoms and diffuse edema gradually resolved in both patients. These two cases of hyperperfusion syndrome occurred in a series of 190 patients treated by clipping of unruptured cerebral aneurysms. Hyperperfusion syndrome is a rare complication following aneurysm surgery, especially surgery for unruptured cerebral aneurysms without temporary clipping.
  • 本望修, 本望修, 寳金清博
    Neurological Surgery 38 6 2010年
  • 村上友宏, 小柳泉, 金子高久, 寳金清博, 恩田敏之, 八巻稔明
    日本脊髄外科学会プログラム・抄録集 25th 2010年
  • 齊藤正樹, 山本和利, 寳金清博, 米増保之, 高橋明, 小松克也, 小松克也, 三上毅, 三上毅, 今井富裕, 矢坂正弘, 岡田靖, 佐藤利夫, 下濱俊
    日本神経学会総会プログラム・抄録集 51st 2010年
  • 三上毅, 秋山幸功, 宮田圭, 南田善弘, 寳金清博
    日本脳神経外科救急学会プログラム・抄録集 15th 2010年
  • Takeshi Mikami, Satoshi Iihoshi, Kiyohiro Houkin
    Moyamoya Disease Update 150 - 157 2010年 [査読有り][通常論文]
     
    Magnetic resonance angiography (MRA) has opened the door to noninvasive evaluation of the intracranial major arteries. The contemporary MRA can clearly disclose the major intracranial arteries and has become an indispensable modality for the management of cerebrovascular stroke. Based on this dramatic technical evolution, since 1994 new diagnostic guidelines for moyamoya disease have included good MRA as the definitive diagnostic technique for moyamoya disease [1-4]. Nowadays, MRA can be an alternative to conventional angiography in moyamoya disease, and has been acknowledged as a reliable diagnostic tool with high sensitivity and specificity as a result of the remarkable development of MR imaging technology [5-13]. Moreover, MRA is useful not only for the diagnosis but also for follow-up of moyamoya disease in a noninvasive way. © 2010 Springer-Verlag Tokyo.
  • Kiyohiro Houkin, Satoshi Iihoshi, Takeshi Mikami
    Moyamoya Disease Update 141 - 149 2010年 [査読有り][通常論文]
     
    As mentioned in the Introduction, when the original concept of moyamoya disease is reviewed, digital subtraction angiography (DSA) is still the most reliable diagnostic modality. However, as mentioned in the following sections, as far as the depiction of the steno-occlusive change of the Willis ring is concerned, other modalities such as magnetic resonance angiography (MRA) and 3-dimensional computed tomography angiography (3D-CTA) with best quality is quite compatible with DSA. However, the depiction of the moyamoya vessels and the collateral circulation is not always clear in MRA and magnetic resonance imaging (MRI). In addition, 3D-CTA essen tially depicts the morphological aspect of vasculature that is not necessarily identical to the true circulation of the blood flow. In addition, the collateral circulation such as (1) basal moyamoya, and (2) transdural anastomosis of the meningeal artery including the vault moyamoya and ethmoidal moyamoya is not well demonstrated in other modalities (Figs.1 and 2). © 2010 Springer-Verlag Tokyo.
  • Masanori Sasaki, Christine Radtke, Andrew M. Tan, Peng Zhao, Hirofumi Hamada, Kiyohiro Houkin, Osamu Honmou, Jeffery D. Kocsis
    JOURNAL OF NEUROSCIENCE 29 47 14932 - 14941 2009年11月 [査読有り][通常論文]
     
    Transplantation of mesenchymal stem cells (MSCs) derived from bone marrow has been shown to improve functional outcome in spinal cord injury (SCI). We transplanted MSCs derived from human bone marrow (hMSCs) to study their potential therapeutic effect in SCI in the rat. In addition to hMSCs, we used gene-modified hMSCs to secrete brain-derived neurotrophic factor (BDNF-hMSCs). After a dorsal transection lesion was induced at T9, cells were microinjected on each side of the transection site. Fluorogold (FG) was injected into the epicenter of the lesion cavity to identify transected corticospinal tract (CST) neurons. At 5 weeks after transplantation, the animals were perfused. Locomotor recovery improvement was observed for the BDNF-hMSC group, but not in the hMSC group. Structurally there was increased sprouting of injured corticospinal tract and serotonergic projections after hMSC and BDNF-hMSC transplantation. Moreover, an increased number of serotonergic fibers was observed in spinal gray matter including the ventral horn at and below the level of the lesion, indicating increased innervation in the terminal regions of a descending projection important for locomotion. Stereological quantification was performed on the brains to determine neuronal density in primary motor (M1) cortex. The number of FG backfilled cells demonstrated an increased cell survival of CST neurons in M1 cortex in both the hMSC and BDNF-hMSC groups at 5 weeks, but the increase for the BDNF-hMSC group was greater. These results indicate that transplantation of hMSCs hypersecreting BDNF results in structural changes in brain and spinal cord, which are associated with improved functional outcome in acute SCI.
  • Ryuichi Katada, Yoko Nishitani, Osamu Honmou, Shunichiro Okazaki, Kiyohiro Houkin, Hiroshi Matsumoto
    JOURNAL OF NEUROTRAUMA 26 11 2015 - 2025 2009年11月 [査読有り][通常論文]
     
    Alcohol consumption prior to traumatic brain injury (TBI) promotes morbidity and mortality although the mechanisms involved remain unclear. The morbidity and mortality caused by TBI, especially brain contusion, are known to be closely associated with brain edema. Here we examined the effects of ethanol pretreatment on brain edema, inflammatory responses, and oxidative stress after brain contusion. Male Wistar rats were given 3g/kg ethanol intraperitoneally and 1 h later were subjected to brain contusion. The ethanol-pretreated group had a significantly decreased survival rate. Magnetic resonance imaging showed ethanol pretreatment significantly augmented the volume of cytotoxic brain edema after contusion. In the ethanol-pretreated rat, the activities of NF-kappa B and AP-1 were reduced 6 h after contusion and COX-2 mRNA expression was increased 24 h after contusion. These findings suggest that ethanol augmented cerebral edema and mortality in rats with brain contusion, possibly through actions on cell survival pathways or COX-2 expression. In addition, antioxidant treatment at 3 h post-injury significantly attenuated some markers of oxidative stress, mortality, and volume of edema at 24 h after ethanol treatment and contusion.
  • Kiyohiro Houkin, Takeo Baba, Yoshihiro Minamida, Tadashi Nonaka, Izumi Koyanagi, Satoshi Iiboshi
    NEUROSURGERY 65 3 587 - 594 2009年09月 [査読有り][通常論文]
     
    OBJECTIVE: it is well recognized that the occurrence rate of adverse events related to surgical procedures is considerably high in neurosurgery compared with other specialties. The purpose of this study was to quantitatively determine the occurrence rate of adverse events related to surgery and endovascular intervention in neurosurgery. METHODS: A conference on adverse events related to treatments (morbidity and mortality conference) has been held every month for the past 2 years in our department. At these conferences, all adverse events are evaluated and discussed. Adverse events include not only the unexpected complications, but also the neurological and general deterioration predicted before surgery. All the adverse events are discussed in terms of the conceivable causes, their association with the procedures, and the possibility of prediction and avoidance. RESULTS: One hundred eighty-two events (28.3%) among 643 neurosurgical interventions over 2 years were recognized as adverse events. Among these 182 adverse events, 165 (90.7%) were closely related to procedures and 125 events (68.7%) were predictable before or during the procedures. However, even when retrospectively reviewed, only 6 (3.3%) of events were deemed avoidable. Of these 6 avoidable events, there were only 2 (1.1%) that were considered to have been caused by error. CONCLUSION: Adverse events are not invariably rare in neurosurgery. Most of them are predictable; however, their avoidance is not necessarily easy. Avoidable adverse events caused by medical errors were observed in only 1.1% of cases.
  • Misuzu Ohsaka, Masayoshi Takigami, Izumi Koyanagi, Sangnyon Kim, Kiyohiro Houkin
    NEUROLOGICAL SURGERY 37 8 797 - 802 2009年08月 [査読有り][通常論文]
     
    A 68-year-old man presented with sudden onset of left hemiparesis, diplopia and numbness of the left side of the body and was admitted to our hospital. MRI of the brain revealed no abnormality. However, neurological examination on admission strongly suggested ischemia of the brainstem. Under anticoagulation therapy his symptoms gradually disappeared by the fourth hospital day. He had a past history of bouts of unconsciousness caused by right rotation of the head, Dynamic plain roentgenograms of the cervical spine showed spondylotic changes and lateral osteophyte formation at C5/6 without instability. Computed tomography (CT)-angiography demonstrated narrowing of the right vertebral artery due to compression of the lateral osteophyte at C5/6. Vertebral angiography revealed complete occlusion of the right vertebral artery induced by right rotation of the head. The artery-to-artery embolism caused by repeated occlusion of the right vertebral artery due to the neck motion was suggested as the mechanism of ischemic attack in this patient, Osteophytectomy at C5/6 via the anterior approach successfully treated dynamic occlusion of the right vertebral artery. Occlusion of the vertebral artery at C1/2 by the head rotation is well known as Bow-Hunter's syndrome. However, dynamic occlusion due to spondylotic changes at C5/6 is rare. In case of ischemic attack of the posterior circulation with lateral osteophyte formation of the cervical spine, dynamic occlusion mechanisms of the vertebral artery at the lower cervical level should be considered.
  • He Liu, Izumi Koyanagi, Hideki Chiba, Masahiro Wanibuchi, Osamu Honmou, Toshiaki Yamaki, Kiyohiro Houkin
    JOURNAL OF CLINICAL NEUROSCIENCE 16 4 570 - 573 2009年04月 [査読有り][通常論文]
     
    Intravascular malignant lymphomatosis (IML) is an extremely rare form of lymphoproliferative disorder that is characterized by proliferation of the lymphoma cells in the vascular lumens. We report oil a 78-year-old man who initially presented with spinal cord infarct at the T1 1/12 level. Despite biopsies of the spinal cord and bone marrow from the sternum, the correct diagnosis was not made until autopsy. We identified 19 patients with IML showing spinal cord symptoms as the initial clinical manifestation, and considered the clinical and radiological characteristics as well as the limitations of biopsy. IML can be an important differential diagnosis of progressive paraparesis of unknown etiology. (c) 2008 Elsevier Ltd. All rights reserved.
  • Koichi Haraguchi, Kiyohiro Houkin, Izumi Koyanagi, Tadashi Nonaka, Takeo Baba
    JOURNAL OF NEUROIMAGING 19 2 174 - 178 2009年04月 [査読有り][通常論文]
     
    We report a unique case of a reversible high signal intensity lesion observed on a magnetic resonance (MR) image accompanied by transient neurological deficits related to a balloon occlusion test. This abnormality was considered to be vasogenic edema caused by the disruption of the blood-brain barrier (BBB) due to a long history of uncontrolled hypertension and transient ischemia induced by the balloon occlusion test. J Neuroimaging 2009;19:174-178.
  • Kentaro Toyama, Osamu Honmou, Kuniaki Harada, Junpei Suzuki, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
    EXPERIMENTAL NEUROLOGY 216 1 47 - 55 2009年03月 [査読有り][通常論文]
     
    Intravenous transplantation of human mesenchymal stem cells (hMSCs) expanded from adult bone marrow ameliorates functional deficits in rat cerebral infarction models. Several hypotheses to account for the therapeutic mechanisms have been suggested, but angiogenesis is thought to be of critical importance. Recently, we have reported the therapeutic benefits of hMSCs which have been transfected with the angiopoietin-1 gene in a rat permanent middle cerebral artery occlusion (MCAO) model. To potentially enhance the therapeutic effects of angiopoietin-1 gene-modified hMSC (Ang-hMSC), we transfected hMSCs with the angiopoietin-1 gene and the VEGF gene, and investigated whether the combination of Ang-1 and VEGF gene-modified hMSCs (Ang-VEGF-hMSC) contribute to functional recovery in a rat MCAO model. We induced MCAO using intraluminal vascular occlusion, and hMSCs, Ang-hMSCs, VEGF-hMSCs or Ang-VEGF-hMSCs were intravenously infused 6 h later. MRI and behavioral analyses revealed that rats receiving Ang-VEGF-hMSCs showed the greatest structural-functional recovery as compared to the other groups. These results suggest that intravenous administration of hMSCs transfected with the angiopoietin-1 and VEGF gene using a fiber-mutant adenovirus vector may represent a new strategy for the treatment of ischemia. (C) 2008 Elsevier Inc. All rights reserved.
  • Akiyama Y, Miyata K, Harada K, Minamida Y, Nonaka T, Koyanagi I, Asai Y, Houkin K
    Neurologia medico-chirurgica 49 3 97 - 99 3 2009年03月 [査読有り][通常論文]
     
    The sensitivity of susceptibility-weighted magnetic resonance (MR) imaging was compared with conventional MR sequences, including T(2)*-weighted imaging, and computed tomography for the detection of cerebral hemorrhages in 15 patients with head injury. Susceptibility-weighted imaging detected a mean of 76+/-52 (total 1132) hypointense spotty lesions, compared to a mean of 21+/-19 (total 316) detected by T(2)*-weighted imaging (p<0.0001, paired t-test). Susceptibility-weighted imaging is extremely sensitive for the visualization and detection of microhemorrhages.
  • Yukinori Akiyama, Kei Miyata, Kuniaki Harada, Yoshihiro Minamida, Tadashi Nonaka, Izumi Koyanagi, Yasufumi Asai, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 49 3 97 - 99 2009年03月 [査読有り][通常論文]
     
    The sensitivity of susceptibility-weighted magnetic resonance (MR) imaging was compared with conventional MR sequences, including T(2)*-weighted imaging, and computed tomography for the detection of cerebral hemorrhages in 15 patients with head injury. Susceptibility-weighted imaging detected a mean of 76 +/- 52 (total 1132) hypointense spotty lesions, compared to a mean of 21 +/- 19 (total 316) detected by T(2)*-weighted imaging (p < 0.0001, paired t-test). Susceptibility-weighted imaging is extremely sensitive for the visualization and detection of microhemorrhages.
  • 飯星智史, 寳金清博
    ウィリス動脈輪閉塞症における病態・治療に関する研究 平成20年度 総括・分担研究報告書 2009年
  • 齊藤正樹, 米増保之, 山本和利, 姉川敬裕, 高橋明, 岡村耕一, 矢坂正弘, 岡田靖, 下濱俊, 寳金清博
    日本脳神経外科救急学会プログラム・抄録集 14th 2009年
  • 飯星智史, 宮田圭, 斉藤正樹, 野中雅, 小柳泉, 寳金清博
    日本神経放射線学会プログラム・抄録集 38th 2009年
  • Shiro Miyata, Takeshi Mikami, Yoshihiro Minamida, Yukinori Akiyama, Kiyohiro Houkin
    JOURNAL OF NEURO-OPHTHALMOLOGY 28 4 325 - 326 2008年12月 [査読有り][通常論文]
     
    A 59-year-old woman presented with disturbance of consciousness and decreased visual acuity caused by a suprasellar mass identified on MRI. A bifrontal interhemispheric approach allowed removal of the top an d lateral sides of the tumor from the wall of the third ventricle. The hypothalamus appeared to be the origin of the mass, which proved to be hemangioblastoma, a rare tumor in this location.
  • Satoshi Kuroda, Kiyohiro Houkin
    LANCET NEUROLOGY 7 11 1056 - 1066 2008年11月 [査読有り][通常論文]
     
    Moyamoya disease is an uncommon cerebrovascular disease that is characterised by progressive stenosis of the terminal portion of the internal carotid artery and its main branches. The disease is associated with the development of dilated, fragile collateral vessels at the base of the brain, which are termed moyamoya vessels. The incidence of moyamoya disease is high in east Asia, and familial forms account for about 15% of patients with this disease. Moyamoya disease has several unique clinical features, which include two peaks of age distribution at 5 years and at about 40 years. Most paediatric patients have ischaemic attacks, whereas adult patients can have ischaemic attacks, intracranial bleeding, or both. Extracranial-intracranial arterial bypass, including anastomosis of the superficial temporal artery to the middle cerebral artery and indirect bypass, can help prevent further ischaemic attacks, although the beneficial effect on haemorrhagic stroke is still not clear. In this Review, we summarise the epidemiology, aetiology, clinical features, diagnosis, surgical treatment, and outcomes of moyamoya disease. Recent updates and future perspectives for moyamoya disease will also be discussed.
  • Hirokuni Hashikata, Wanyang Liu, Yohei Mineharu, Kayoko Inoue, Katsunobu Takenaka, Hidetoshi Ikeda, Kiyohiro Houkin, Satoshi Kuroda, Kenji Kikuchi, Mitsuru Kimura, Takuyuu Taki, Makoto Sonobe, Sadahiko Ban, Hidekazu Nogaki, Akira Handa, Kenichiro Kikuta, Yasushi Takagi, Kazuhiko Nozaki, Nobuo Hashimoto, Akio Koizumi
    Brain and Nerve 60 11 1261 - 1269 2008年11月 [査読有り][通常論文]
     
    Moyamoya disease (MMD) is characterized by progressive stenosis and occlusion of the terminal portion of the bilateral carotid arteries as well as arterial collateral vessels. The etiology of MMD, however, remains unknown. Several pieces of evidence suggest the involvement of genetic factors in MMD: over 10% of MMD patients have affected blood relatives concordance in the affection status has been proven in 80% of identical twins and there is an ethnic predisposition to MMD, the incidence of the disease being the highest in the Asian population. With regard to genetic factor (s), transmission of MMD does not follow the classic Mendelian law, i, e., skipping of a generation and discordance in identical twins, thereby indicating that genetic influence is likely to determine the susceptibility to MMD. This study aimed to overview the recent findings related to the genetic determinants in MMD and to provide research perspectives for the next decade. Pathophysiological investigations at molecular levels have uncovered the upregulation of various growth and stress response factors that are associated with angiogenesis in occlusive cerebral arteries. Genetic studies have been conducted in the past 30 years. In the first phase, the association of MMD with HLA was investigated extensively, but the opinions remained equivocal. In the second phase, linkage analysis was performed, which demonstrated multiple loci-3p24.2-26, 6q, 8q23. 12p12, and 17q25. None of these studies were replicated. A large genome-wide linkage analysis using 3 generation families that has been performed in 2008 has resolved the enigma of MMD and revealed a single locus on17q25. This locus is expected to provide a clue to the genetic basis for MMD, paving a way to comprehensive understanding of molecular consequences in MMD.
  • 黒田 敏, 中山 若樹, 石川 達哉, 宝金 清博, 岩崎 喜信
    脳循環代謝 20 1 153 - 153 (一社)日本脳循環代謝学会 2008年11月 [査読無し][通常論文]
  • Yoshinori Omori, Osamu Honmou, Kuniaki Harada, Junpei Suzuki, Kiyohiro Houkin, Jeffery D. Kocsis
    BRAIN RESEARCH 1236 30 - 38 2008年10月 [査読有り][通常論文]
     
    The systemic injection of human mesenchymal stem cells (hMSCs) prepared from adult bone marrow has therapeutic benefits after cerebral artery occlusion in rats, and may have multiple therapeutic effects at various sites and times within the lesion as the cells respond to a particular pathological microenvironment. However, the comparative therapeutic benefits of multiple injections of hMSCs at different time points after cerebral artery occlusion in rats remain unclear. in this study, we induced middle cerebral artery occlusion (MCAO) in rats using intra-luminal vascular occlusion, and infused hMSCs intravenously at a single 6 h time point (low and high cell doses) and various multiple time points after MCAO. From MRI analyses lesion volume was reduced in all hMSC cell injection groups as compared to serum alone injections. However, the greatest therapeutic benefit was achieved following a single high cell dose injection at 6 h post-MCAO, rather than multiple lower cell infusions over multiple time points. Three-dimensional analysis of capillary vessels in the lesion indicated that the capillary volume was equally increased in all of the cell-injected groups. Thus, differences in functional outcome in the hMSC transplantation subgroups are not likely the result of differences in angiogenesis, but rather from differences in neuroprotective effects. (C) 2008 Elsevier B.V. All rights reserved.
  • Y. Akiyama, I. Koyanagi, K. Yoshifuji, T. Murakami, T. Baba, Y. Minamida, T. Nonaka, K. Houkin
    JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY 79 10 1153 - 1158 2008年10月 [査読有り][通常論文]
     
    Object: The pathophysiology of syringomyelia in Chiari type 1 malformations has not been clarified. Oedema-like spinal-cord swelling was recently reported in several pathological conditions, including Chiari type 1 malformations as a pre-syrinx state. However, the role of the pre-syrinx state in the development of syringomyelia is unknown. The purpose of this study is to investigate the parenchymal changes of the spinal cord in syringomyelia associated with Chiari type 1 malformations. Methods: Pre- and postoperative MRI findings in 14 patients who underwent foramen magnum decompression in our institute were reviewed. The analysis was focused on differences in visualisation of the syrinx between T1- and T2-weighted images and abnormal parenchymal signal changes. There were 6 men and 8 women, aged from 6 to 79 years. No patients showed hydrocephalus. Results: Twelve patients had large and expansive syrinx, whereas 2 patients showed small syrinx confined to the centre of the spinal cord. T2-weighted images displayed significantly larger intramedullary abnormal signal areas. Nine patients showed parenchymal hyperintensity areas around the enlarged central canal or base of the posterior white columns adjacent to the syringomyelic cavity. Such parenchymal hyperintensity areas markedly diminished with reduction of the syrinx after surgery and were considered to be interstitial oedema. Conclusions: From this study, the interstitial oedema of the spinal cord commonly accompanies syringomyelia with Chiari type 1 malformations. Accumulation of the extracellular fluid due to disturbed absorption mechanisms may play an important role in the pathophysiology of syringomyelia associated with Chiari type 1 malformations.
  • Izumi Koyanagi, Kazutoshi Hida, Yoshinobu Iwasaki, Toyohiko Isu, Masami Yoshino, Tomohiro Murakami, Kazuhisa Yoshifuji, Kiyohiro Houkin
    NEUROSURGERY 63 3 546 - 551 2008年09月 [査読有り][通常論文]
     
    OBJECTIVE: A significant variety in morphology of conus lipomas may underlie differences in clinical presentation of the patients and controversy in surgical management. We retrospectively studied 58 patients with conus lipomas at our institutions. The purpose of this study was to infer the clinical course from the radiological findings and to provide information for decision-making in planning for surgical treatment. METHODS: The patients underwent untethering surgery between 1984 and 2005. There were 35 transitional and 23 dorsal lipomas. The age at surgery ranged from 1 month to 50 years (median, 4 yr). Preoperative clinical history, radiological findings, and postoperative results were analyzed. RESULTS: Fifteen patients were asymptomatic, and 43 patients were symptomatic pre-operatively. Twenty-one patients presented with motor deficits of the lower extremities. In seven patients, motor deficits appeared early, before 1 year of age. Massive lipomas compressing the cord or herniation of the spinal cord into the subcutaneous tissue were characteristic findings of such early deterioration. Motor deficits were present in 73% of patients with lipomas extending to the lumbar level, whereas 88% of patients with lipomas confined to the sacral level had only urinary deficits. During a mean postoperative follow-up period of 7.9 years, 4 (27%) of the 15 asymptomatic patients developed urinary and/or motor deficits, and 12 (28%) of the 43 symptomatic patients showed further neurological deterioration. CONCLUSION: This study demonstrates that the location and morphology of conus lipomas influence the neurological presentation of the patients. Early prophylactic surgery is a reasonable treatment option if early deterioration is predicted by imaging studies.
  • T. Baba, K. Houkin, S. Kuroda
    JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY 79 8 900 - 904 2008年08月 [査読有り][通常論文]
     
    Background: Many clinical features that are specific to moyamoya disease have been reported and cited in textbooks based on previous data. The purpose of this study is to investigate the present epidemiological features of moyamoya disease based on recently obtained regional all-inclusive data. Methods: The authors performed an all-inclusive survey of moyamoya disease in Hokkaido, one of the major islands in Japan that has a population of 5.63 million. The epidemiological features were analysed based on the data from 267 newly registered patients with moyamoya disease in Hokkaido from 2002 to 2006. These analysed data were adjusted to the whole Japanese population at 2005. Results: The detection rate of the disease per year was 0.94 patients per 100 000 people, and prevalence was 10.5 patients per 100 000 people. The incidence of ischaemia concerned with the disease was 0.53 patients per 100 000 people- years and haemorrhage was 0.2 patients per 100 000 people- years. The ratio of female to male patients was 2.18. The ratio of patients aged 10 years and above to under 10 years of age at onset was 6.18. Two peaks for age of onset were seen: the highest was observed between 45 and 49 years, and the second between 5 and 9 years. Asymptomatic patients comprised 17.8% of the total number of patients. Conclusion: The epidemiological features of moyamoya disease determined by this survey varied considerably from previous data. The detection rate and prevalence of the disease were higher than those reported previously. The highest peak of onset age was older than those reported previously. In addition, it was revealed that asymptomatic moyamoya patients are not always rare in Japan.
  • Mineharu Y, Liu W, Inoue K, Matsuura N, Inoue S, Takenaka K, Ikeda H, Houkin K, Takagi Y, Kikuta K, Nozaki K, Hashimoto N, Koizumi A
    Neurology 70 24 Pt 2 2357 - 2363 2008年06月 [査読有り][通常論文]
  • K. Haraguchi, K. Houkin, I. Koyanagi, T. Nonaka, T. Baba
    MINIMALLY INVASIVE NEUROSURGERY 51 2 91 - 94 2008年04月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate quantitatively the composition of carotid plaque by computed tomographic (CT) angiography and qualitatively by black blood magnetic resonance images (MRI). Thirty-eight patients with high-grade carotid artery stenosis were included in this study. Ultrasonography, CT angiography and black blood MRI of the cervical carotid artery were performed, and the CT number was measured in Hounsfield units (HU). The average CT number of the 15 unstable plaques (39.5%) was 27.7 +/- 7.5 HU and that of the 23 stable plaques (60.5%) was 60.4 +/- 20.8 HU (p=0.0001). In the 23 patients with stable plaque, 21 demonstrated isointensity in T-1- and T-2-weighted images in the black blood MRI (p = 0.0001). By using CT angiography and MRI, precise images of the pathology of the carotid arterial wall can be obtained. It is possible to evaluate the components of carotid artery plaque with high reliability by quantification of the CT number in CT angiography and performing black blood MRI as well as carotid ultrasonography.
  • K. Haraguchi, K. Houkin, I. Koyanagi, T. Nonaka, T. Baba
    INTERVENTIONAL NEURORADIOLOGY 14 1 39 - 43 2008年03月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the composition of a carotid plaque quantitatively by computed tomography (CT) angiography and qualitatively by black blood magnetic resonance imaging (MRI). Thirty-eight patients with high-grade carotid artery stenosis were included in this study. Ultrasonography, CT angiography and black blood MRI of the cervical carotid artery were performed, and the CT number was measured in Hounsfield units (HU). The average CT number of the 15 unstable plaques (39.5%) was 27.7 +/- 7.5 HU and that of the 23 stable plaques (60.5%) was 60.4 +/- 20.8 HU (p <0.0001). In the 23 patients with stable plaque, 21 demonstrated isointensity in T1 and T2 in the black blood MRI (p <0.0001). By using CT angiography and MRI, precise images of the pathology of the carotid arterial wall can be obtained. It is possible to evaluate the components of a carotid artery plaque with high reliability by quantification of the CT number in CT angiography and performing black blood MRI as well as in carotid ultrasonography.
  • Toshiyuki Onda, Osamu Honmou, Kuniaki Harada, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
    JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM 28 2 329 - 340 2008年02月 [査読有り][通常論文]
     
    Transplantation of human mesenchymal stem cells (hMSCs) prepared from adult bone marrow has been reported to ameliorate functional deficits after cerebral artery occlusion in rats. Although several hypotheses to account for these therapeutic effects have been suggested, current thinking is that both neuroprotection and angiogenesis are primarily responsible. In this study, we compared the effects of hMSCs and angiopoietin-1 gene-modified hMSCs (Ang-hMSCs) intravenously infused into rats 6 h after permanent middle cerebral artery occlusion. Magnetic resonance imaging and histologic analyses revealed that rats receiving hMSCs or Ang-hMSCs exhibited comparable reduction in gross lesion volume as compared with the control group. Although both cell types indeed improved angiogenesis near the border of the ischemic lesions, neovascularization and regional cerebral blood flow were greater in some border areas in Ang-hMSC group. Both hMSC- and Ang-hMSC-treated rats showed greater improved functional recovery in the treadmill stress test than did control rats, but the Ang-hMSC group was greater. These results indicate the intravenous administration of genetically modified hMSCs to express angiopoietin has a similar effect on reducing lesion volume as hMSCs, but the Ang-hMSC group showed enhanced regions of increased angiogenesis at the lesion border, and modest additional improvement in functional outcome.
  • Toshiya Osanai, Satoshi Kuroda, Naoki Nakayama, Tomohiro Yamauchi, Kiyohiro Houkin, Yoshinobu Iwasaki
    Surgical neurology 69 2 197 - 200 2008年02月 [査読有り][通常論文]
     
    BACKGROUND: In moyamoya disease, intracranial bleeding is known to occur because of the rupture of saccular aneurysms in the circle of Willis or because of the rupture of dilated, fragile moyamoya vessels. The former causes subarachnoid hemorrhage (SAH), and the latter causes intracerebral or intraventricular hemorrhage. CASE DESCRIPTION: In this report, we describe the case of a 34-year-old woman with moyamoya disease who suddenly developed headache and jacksonian seizure. Plain computed tomographic scans on admission revealed SAH localized over the left frontal cortex. The patient was diagnosed with moyamoya disease on cerebral angiography. However, no aneurysm was found on cerebral angiography. Positron emission tomography showed the reduction of CBF and its reactivity to acetazolamide and the elevation of CBV in the left hemisphere. She underwent STA to MCA anastomosis and indirect synangiosis. Intraoperative observations revealed that the pial arterioles were markedly dilated on the brain surface. The CBF in the left hemisphere significantly improved after surgery. The patient has experienced no further episode of cerebral ischemia or intracranial bleeding. CONCLUSIONS: Subarachnoid hemorrhage of unknown cause is quite rare in moyamoya disease. Based on the findings in the present case, the dilated collateral arteries on the brain surface may rupture and cause SAH over the cerebral cortex, which is the third cause of intracranial bleeding in patients with persistent cerebral ischemia due to moyamoya disease.
  • Autosomal dominant moyamoya disease maps to chromosome 17q25.3. Neurology. 2008 Jun 10;70(24 Pt 2):2357-63. Epub 2008 May 7.
    Mineharu Y, Liu W, Inoue K, Matsuura N, Inoue S, Takenaka K, Ikeda H, Houkin K, Takagi Y, Kikuta K, Nozaki K, Hashimoto N, Koizumi A
    2008年 [査読有り][通常論文]
  • Tadashi Nonaka, Koichi Haraguchi, Takeo Baba, Izumi Koyanagi, Kiyohiro Houkin
    SURGICAL NEUROLOGY 67 6 612 - 619 2007年06月 [査読有り][通常論文]
     
    Background: Clipping a paraclinoid aneurysm is difficult if the patient has a visual disturbance. Visual function sometimes deteriorates postoperatively for patients with a large aneurysm. In this study, we report the long-term follow-up of patients with visual impairments attributed to optic nerve compression when paraclinoid aneurysms are surgically treated. Methods: Seventeen patients with optic nerve impairment induced by compression of paraclinoid ICA aneurysms were treated. All of the aneurysms were large, including 6 giant aneurysms. The aneurysms displayed partial thrombosis or calcification of the aneurysmal wall in 6 cases. Results: Direct surgery such as neck clipping or wrapping of the aneurysm was performed in 9 aneurysms and indirect procedures in 8 others (ICA occlusion, 1; ICA occlusion + bypass, 7). Of 17 patients, 11 (65%) showed improvement in several dysfunctions of visual acuity or visual field. Of 6 patients, whose vision had not recovered well, 5 underwent direct surgery. Moreover, these 5 patients had an intra-aneurysmal thrombosis or calcification of the aneurysmal wall. Nevertheless, I patient whose aneurysm with partial thrombosis was treated via indirect procedure had good recovery of vision. Conclusions: Direct clipping is the treatment of choice for patients with a mass effect on the optic nerve due to paraclinoid aneurysm. However, it is difficult to achieve sufficient decompression of the optic nerve when the aneurysm is accompanied by partial thrombosis or calcification of the aneurysmal wall. In those cases, an indirect procedure seems to be a relatively safe, effective treatment. (C) 2007 Elsevier Inc. All rights reserved.
  • Ryo Ukai, Osamu Honmou, Kuniaki Harada, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
    JOURNAL OF NEUROTRAUMA 24 3 508 - 520 2007年03月 [査読有り][通常論文]
     
    Intravenous delivery of mesenchymal stem cells (MSCs) prepared from bone marrow (BMSCs) reduces infarction volume and ameliorates functional deficits in a rat cerebral ischemia model. MSC-like multipotent precursor cells (PMSCs) have also been suggested to exist in peripheral blood. To test the hypothesis that treatment with PMSCs may have a therapeutic benefit in stroke, we compared the efficacy of systemic delivery of BMSCs and PMSCs. A permanent middle cerebral artery occlusion (MCAO) in rat was induced by intraluminal vascular occlusion with a microfilament. Rat BMSCs and PMSCs were prepared in culture and intravenously injected into the rats 6 h after MCAO. Lesion size was assessed at 6 h, and 1, 3, and 7 days using MR imaging and histology. The hemodynamic change of cerebral blood perfusion on stroke was assessed the same times using perfusion-weighted image (PWI). Functional outcome was assessed using the treadmill stress test. Both BMSCs and PMSCs treated groups had reduced lesion volume, improved regional cerebral blood flow, and functional improvement compared to the control group. The therapeutic benefits of both MSC-treated groups were similar. These data suggest that PMSCs derived from peripheral blood could be an important cell source of cell therapy for stroke.
  • Taro Yamashita, Takeshi Mikami, Takeo Baba, Yoshihiro Minamida, Toshiya Sugino, Izumi Koyanagi, Tadashi Nonaka, Kiyohiro Houkin
    JOURNAL OF NEURO-OPHTHALMOLOGY 27 1 48 - 49 2007年03月 [査読有り][通常論文]
     
    An inebriated 86-year-old man impaled himself on a wooden earpick that penetrated through the superior orbital fissure into the prepontine cistern. The patient underwent surgery immediately by a lateral suboccipital approach, and the earpick was pulled out through the wound with control of hemorrhage from the cavernous sinus. He survived this event with no neurologic deficits apart from complete ipsilateral ophthalmoplegia, and ptosis. Prompt imaging and surgical intervention allowing direct visualization of the foreign body and prevention of intracranial complications are part of proper management of this problem.
  • Minamida Y, Houkin K
    Nihon rinsho. Japanese journal of clinical medicine 65 Suppl 3 573 - 578 2007年03月 [査読有り][通常論文]
  • Kuniaki Harada, Osamu Honmou, He Liu, Michio Bando, Kiyohiro Houkin, Jeffery D. Kocsis
    BRAIN RESEARCH 1134 1 206 - 213 2007年02月 [査読有り][通常論文]
     
    Proton magnetic resonance spectroscopy (1-H MRS) has revealed changes of metabolites in acute cerebral infarction. Although the drastic changes of lactate and N-acetyl-aspartate have been reported to be useful indicators of the ischemic damage in both humans and experimental animals, lipid signals are also detected by the short echo time sequence 15 days after ischemia. The objective of this study was to find a novel technique to isolate lactate signals from lipid signals in the ischemic brain. First, MRS was used to study the lipid and lactate components of a spherical phantom in vitro, and parameters were established to separate these components in vitro. Then, MR measurements were obtained from the brains of middle cerebral artery occlusion rats. All MR measurements were performed using a 7-T (300 MHz), 18.3-cm-bore superconducting magnet (Oxford Magnet Technologies) interfaced to a Unity INOVA Imaging System (Varian Technologies). T-2-weighted images were obtained from a 1.0-mm-thick coronal section using a 3-cm field of view. It is well known that lipid has a shorter and lactate a longer T-2 relaxation time. These distinct magnetic characteristics allowed us to separate the lactate signal from the lipid signal. Thus, adjustment of the echo time is essential to analyze the metabolites in acute cerebral infarction, which maybe useful in both the clinic and laboratory. (c) 2006 Elsevier B.V. All rights reserved.
  • Takeshi Mikami, Yoshihiro Minamida, Toshiya Sugino, Izumi Koyanagi, Takatoshi Yotsuyanagi, Kiyohiro Houkin
    Operative Neurosurgery 60 suppl_2 ONS - 83 2007年02月01日 [査読有り][通常論文]
     
    Abstract Objective: Postcraniotomy subdural empyemas and epidural abscesses are uncommon, potentially lethal, complications of neurosurgery. Patients with these complications may be difficult to manage, and mortality can occur. Methods: Between 1997 and 2006, the authors treated eight patients with recalcitrant postcraniotomy subdural empyema and epidural abscess with combinations of myocu-taneous free flap transfer. The free flap transfer was intended for patients who could not be cured with conventional surgical debridement and bone flap removal. Patient ages ranged from 15 to 67 years (mean, 41.5 yr). There were six men and two women. Treatment was required for cranial base tumors (n = :3), the result of trauma (n = :2), malignant tumors (n = :2), and cerebral hematoma (n = :1). In six patients (75%), an expanded polytetrafluoroethylene sheet was used as a dural substitute at the original surgery. We used three rectus abdominis myocutaneous flaps and five latissimus dorsi myocutaneous flaps. In six patients (75%), surgery was performed in the chronic stage of infection, and the other two patients were in the acute stage of infection. RESULTS: Among all the patients, two failures occurred because of flap ischemia, but these were resolved after an additional procedure. However, one of these two patients, in whom surgery was performed at the acute stage of infection, died 4 weeks after the surgery. The postoperative course of the other six patients was uneventful. Isolated microorganisms were methicillin-resistant Staphylococcus aureus (four patients), Pseudomonas aeruginosa(three patients), and methicillin-sensitive Staphylococcus aureus(one patient). Conclusion: Myocutaneous free flap transfer allows sufficient blood circulation and dead space control and is resistant to infection. Therefore, free flap transfer is useful for eliminating intractable empyema and abscess; however, it is important that the procedure be considered in the chronic stage of infection.
  • Takeshi Mikami, Yoshihiro Minamida, Izumi Koyanagi, Kiyohiro Houkin
    Operative Neurosurgery 60 157 - 161 2007年02月 [査読有り][通常論文]
  • Koichi Haraguchi, Kiyohiro Houkin, Tadashi Nonaka, Takeo Baba
    NEUROLOGIA MEDICO-CHIRURGICA 47 2 79 - 82 2007年02月 [査読有り][通常論文]
     
    A 77-year-old woman presented with delayed thromboembolic infarction of a basilar artery trunk aneurysm that occurred 2 weeks after coil embolization of the unruptured aneurysm. The postoperative course was uneventful until the patient suffered sudden onset of consciousness disturbance 13 days after the treatment. Computed tomography showed no abnormality, but digital subtraction angiography revealed a thrombus extending from the aneurysm neck to a point distal to the basilar artery. Thrombolysis was achieved by the infusion of urokinase. Reconfiguration of the coil at the aneurysm neck was revealed compared with the initial configuration of the coil immediately after embolization. Magnetic resonance imaging obtained after the thrombolysis showed hyperintense areas in the bilateral occipital lobes and thalami. Periprocedural thromboembolic complications associated with coil embolization of an aneurysm are well known. However, delayed thromboembolic complications may occur in some patients after successful coil packing of an aneurysm.
  • Takeshi Mikami, Yoshihiro Minamida, Toshiya Sugino, Izumi Koyanagi, Takatoshi Yotsuyanagi, Kiyohiro Houkin
    NEUROSURGERY 60 2 83 - 87 2007年02月 [査読有り][通常論文]
     
    OBJECTIVE: Postcraniotomy subdural empyemas and epidural abscesses are uncommon, potentially lethal, complications of neurosurgery. Patients with these complications may be difficult to manage, and mortality can occur.METHODS: Between 1997 and 2006, the authors treated eight patients with recalcitrant postcraniotomy subdural empyema and epidural abscess with combinations of myocutaneous free flap transfer. The free flap transfer was intended for patients who could not be cured with conventional surgical debridement and bone flap removal. Patient ages ranged from 15 to 67 years (mean, 41.5 yr). There were six men and two women. Treatment was required for cranial base tumors. (n = 3), the result of trauma (n = 2), malignant tumors (n = 2), and cerebral hematoma (n = 1). In six patients (75%), an expanded polytetrafluoroethylene sheet was used as a dural substitute at the original surgery. We used three rectus abdominis myocutaneous flaps and five latissimus dorsi myocutaneous flaps. In six patients (75%), surgery was performed in the chronic stage of infection, and the other two patients were in the acute stage of infection.RESULTS: Among all the patients, two failures occurred because of flap ischemia, but these were resolved after an additional procedure. However, one of these two patients, in whom surgery was performed at the acute stage of infection, died 4 weeks after the surgery. The postoperative course of the other six patients was uneventful. Isolated microorganisms were methicillin-resistant Staphylococcus aureus (four patients), Pseudomonas aeruginosa (three patients), and methicillin-sensitive Staphylococcus aureus (one patient).CONCLUSION: Myocutaneous free flap transfer allows sufficient blood circulation and dead space control and is resistant to infection. Therefore, free flap transfer is useful for eliminating intractable empyema and abscess; however, it is important that the procedure be considered in the chronic stage of infection.
  • Takeshi Mikami, Yoshihiro Minamida, Izumi Koyanagi, Kiyohiro Houkin
    NEUROSURGERY 60 2 157 - 160 2007年02月 [査読有り][通常論文]
     
    Objective: Advancement in new technology has been necessary for the development of surgical hemostasis. The purpose of this study was to assess novel bipolar forceps plated with gold-polytetrafluoroethylene (PTEE) composite film. Methods: Novel bipolar forceps with gold-PTFE composite film were invented and compared with commercially available forceps with 50-mu m gold-plated tips in terms of their physical properties and coagulation performance. The contact angle was measured to determine the degree of wetting tension. Then the surface roughness, electric resistance and changes in local temperature on the surface of the tip were recorded. Coagulation performance of the two sets of bipolar forceps was determined comparing the amount of protein in the adhered coagulum on the tips and comparing the ease with which the coagulum could be removed. Results: This gold-PTFE composite film-plated metal has a significantly higher contact angle than conventional gold-plated metal, and the plating maintains the matrix metal property. The amount of coagulum sticking was smaller than with the ordinary gold-plated forceps. The ease of removal was also superior with the gold-PTFE composite film-plated forceps. Conclusion: The concept of promotion protein repellence is important for achieving reduced adherence of protein to neurosurgical instruments. This protein repellent plating is indispensable to achieve safer and more accurate microneurosurgery.
  • Kuroda Satoshi, Ishikawa Tatsuya, Nakayama Naoki, Houkin Kiyohiro, Iwasaki Yoshinobu
    STROKE 38 2 525 - 526 2007年02月 [査読有り][通常論文]
  • Yukinori Akiyama, Takeo Baba, Yukihiro Ibayashi, Yasufumi Asai, Kiyohiro Houkin
    International Journal of Cardiology 114 3 E93 - E95 2007年01月18日 [査読有り][通常論文]
     
    Alveolar soft part sarcoma (ASPS) is a rare soft tissue neoplasm. The coexistence of ASPS with cardiac metastasis is quite rare, in particular. In general, the sarcoma is a malignant disease and grows very fast. However, the mean survival time of patients with ASPS is relatively long. Patients who are diagnosed with localized disease usually have a favorable prognosis, while those who present with widespread metastases usually have a poor prognosis and ultimately, succumb to their disease. The use of modern treatment modalities, such as combination of surgery, and radiotherapy, may significantly prolong the survival time in many patients. Because of the long-term period with symptomatic brain tumors in this disease, the patients should be treated even in the presence of multiple metastases in other organs. © 2006 Elsevier Ireland Ltd. All rights reserved.
  • Takesm Mikami, Yoshimro Minamma, Immi Koyanagi, Takeo Baba, Kiyomro Houkin
    JOURNAL OF NEUROSURGERY 106 1 170 - 174 2007年01月 [査読有り][通常論文]
     
    Object. An anterior clinoidectomy can provide enormous benefits, facilitating the management of paraclinoid and upper basilar artery lesions, but it also carries the potential risk of cerebrospinal fluid leaks. The aim of this study was to assess the variation in the pneumatization of the anterior clinoid process (ACP) in an attempt to reduce the complications associated with an anterior clinoidectomy. Methods. The authors analyzed the anatomical variations in the pneumatization of the ACP and optical strut (OS) in 600 sides of 300 consecutive patients by using multidetector-row computed tomography (CT). Computed tomography scans with a 0.5-mm thickness were obtained, and coronal and sagittal reconstructions of the images were displayed in all cases. Pneumatization of the ACP was found in 9.2% of all sides. The origin of pneumatization was the sphenoid sinus in 81.8% of all the sides, the ethmoid sinus in 10.9%, and both of these sinuses in 7.3%. Pneumatized patterns were divided into three groups according to the route: 74.5% were Type I, in which pneumatization occurred via the OS; 14.5% were Type II, pneumatization via the anterior root (AR); and 10.9% were Type III, pneumatization via both the OS and the AR. The origin of pneumatization and the pneumatization pattern showed statistical dependence (p < 0.001). Pneumatization of the OS beyond its narrowest point was found in 6.8% of all sides. Conclusions. An awareness of the different variations in pneumatization can prevent destruction of the mucous membrane and facilitate orientation during reconstruction with cranialization. During an anterior clinoidectomy, preoperative CT assessments are necessary to evaluate pneumatization of the ACP.
  • 本望修, 寳金清博
    脳卒中 29 2 2007年
  • 秋山幸功, 南田善弘, 小柳泉, 野中雅, 寳金清博
    日本脳神経外科学会総会抄録集(CD-ROM) 66th 2007年
  • Sangnyon Kim, Osamu Honmou, Kazunori Kato, Tadashi Nonaka, Kiyohiro Houkin, Hirufumi Amada, Jeffery D. Kocsis
    BRAIN RESEARCH 1123 1 27 - 33 2006年12月 [査読有り][通常論文]
     
    Transplantation of mesenchymal stem cells (MSCs) prepared from adult bone marrow (BMSCs) has been reported to ameliorate functional deficits in several CNS diseases in experimental animal models. Bone marrow was enriched in MSCs by selecting for plastic-adherent cells that were grown to confluency in appropriate culture conditions as flattened fibroblast-like cells. Despite the fact that the stem/precursor cells in peripheral blood are widely used for reconstruction in the hematopoietic system, it is not fully understood whether peripheral blood-derived plastic-adherent precursor/stem cells (PMSCs) can differentiate into a neural lineage. To compare the potential of PMSCs and BMSCs for neural differentiation in vitro, BMSCs and PMSCs were prepared from the adult rat and expanded in culture. Although the growth rate of PMSCs was less than BMSCs, immunocytochemical and RT-PCR analyses indicated that both MSC types were successfully induced to nestin-positive neurospheres in the presence of EGF and bFGF. After withdrawal of the mitogens, these cells could differentiate into neurofilament-positive neurons or GFAP-positive glia. Thus, our findings suggest the potential use of PMSCs for a cell therapy in CNS diseases. (c) 2006 Elsevier B.V. All rights reserved.
  • Haraguchi K, Houkin K
    Nihon rinsho. Japanese journal of clinical medicine 64 Suppl 8 361 - 363 2006年11月 [査読有り][通常論文]
  • Yoshifumi Horita, Osamu Honmou, Kuniaki Harada, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
    JOURNAL OF NEUROSCIENCE RESEARCH 84 7 1495 - 1504 2006年11月 [査読有り][通常論文]
     
    Intravenous administration of human mesenchymal stem cells (hMSCs) prepared from adult bone marrow has been reported to ameliorate functional deficits after cerebral artery occlusion in rats. Several hypotheses to account for these therapeutic effects have been suggested, and current thinking is that neuroprotection rather than neurogenesis is responsible. To enhance the therapeutic benefits of hMSCs potentially, we transfected hMSCs with the glial cell line-derived neurotrophic factor (GDNF) gene using a fiber-mutant F/RGD adenovirus vector and investigated whether GDNF gene-modified hMSCs (GDNF-hMSCs) could contribute to functional recovery in a rat permanent middle cerebral artery occlusion (MCAO) model. We induced MCAO by using intraluminal vascular occlusion, and GDNF-hMSCs were intravenously infused into the rats 3 hr later. MRI and behavioral analyses revealed that rats receiving GDNF-hMSCs or hMSCs exhibited increased recovery from ischemia compared with the control group, but the effect was greater in the GDNF-hMSC group. Thus, these results suggest that intravenous administration of hMSCs transfected with the GDNF gene using a fiber-mutant adenovirus vector may be useful in the cerebral ischemia and may represent a new strategy for the treatment of stroke. (c) 2006 Wiley-Liss, Inc.
  • Kuniaki Harada, Osamu Honmou, Yoshihiro Odawara, Michio Bando, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 46 11 523 - 528 2006年11月 [査読有り][通常論文]
     
    The signal-to-noise ratio obtained from arteries in three-dimensional (3D) time-of-flight (TOF) magnetic resonance (MR) angiography is often too low to allow clinical diagnosis because the radiofrequency pulse decreases the magnetization of protons in the blood and suppresses the in-flow effect in the slab. The present study adjusted the position of the head coil to boost arterial signal intensity. Ten healthy volunteers, eight men and two women aged 24-78 years, underwent 3D TOF MR angiography of the intracranial arteries with the same standard GE transmit-receive birdcage head coil using both normal and half position (lower edge of the coil level with the mouth) methods. Our subjects were divided into Group 1 consisted of five relatively young volunteers aged 24-42 years (mean 31.2 years), and Group 2 consisted of five older volunteers aged 70-78 years (mean 73 years). The following four arteries were chosen for analysis: the internal carotid artery (ICA), the proximal middle cerebral artery segment (M-1), and the two distal middle cerebral artery segments (M-2, M-3). The half position method increased the signal-to-noise ratio in the ICA, M-1, M-2, and M-3 by 15%9 25%, 36%, and 44%, respectively. In general, this method resulted in the generation of stronger signals in the M-2 and M-3 in younger subjects and in all arteries examined in older subjects. The half position method can provide better MR angiograms in certain brain regions of younger people, and in all brain regions in older patients.
  • Susumu Chiba, Takeshi Koizumi, Hiroyuki Matsumoto, Misuzu Ohsaka, Tadashi Nonaka, Kiyohiro Houkin
    CLINICAL RHEUMATOLOGY 25 6 911 - 913 2006年11月 [査読有り][通常論文]
     
    Superior saggital sinus thrombosis (SSST), which has a strong causal link with antiphospholipid syndrome, rarely occurs in patients with systemic lupus erythematosus (SLE). We describe a 34-year-old woman with SLE whose clinical problem was mild headache. Her serology indicated negative antiphospholipid, anticardiolipin antibodies and lupus anticoagulants. However, marked dilatation of the entire saggital sinus with scattered thrombi was observed in enhanced-, surface- and three-dimensional reconstructed CTs (3D-CTs) without abnormal intra-axial signal in brain MRI. The enhanced-, surface- and 3D-CTs are useful to detect silent dural sinus dilatation with scattered thrombi in a patient with SLE without any symptoms of SSST.
  • Honmou O, Houkin K
    Nihon rinsho. Japanese journal of clinical medicine 64 Suppl 7 664 - 666 2006年10月 [査読有り][通常論文]
  • Yukinori Akiyama, Yasufumi Asai, Kiyohiro Houkin
    AMERICAN JOURNAL OF EMERGENCY MEDICINE 24 6 746 - 748 2006年10月 [査読有り][通常論文]
  • H. Liu, Osamu Honmou, K. Harada, K. Nakamura, K. Houkin, H. Hamada, J. D. Kocsis
    Brain 129 10 2734 - 2745 2006年10月 [査読有り][通常論文]
     
    Intravenous delivery of mesenchymal stem cells (MSCs) prepared from adult bone marrow reduces infarction size and ameliorates functional deficits in rat cerebral ischaemia models. Placental growth factor (PlGF) is angiogenic to impaired non-neural tissue. To test the hypothesis that PlGF contributes to the therapeutic benefits of MSC delivery in cerebral ischaemia, we compared the efficacy of systemic delivery of human MSCs (hMSCs) and hMSCs transfected with a fibre-mutant F/RGD adenovirus vector with a PlGF gene (PlGF-hMSCs). A permanent middle cerebral artery occlusion (MCAO) was induced by intraluminal vascular occlusion with a microfilament. hMSCs and PlGF-hMSCs were intravenously injected into the rats 3 h after MCAO. Lesion size was assessed at 3 and 6 h, and 1, 3, 4 and 7 days using MR imaging and histology. Functional outcome was assessed using the limb placement test and the treadmill stress test. Both hMSCs and PlGF-hMSCs reduced lesion volume, induced angiogenesis and elicited functional improvement compared with the control sham group, but the effect was greater in the PlGF-hMSC group. Enzyme-linked immunosorbent assay of the infarcted hemisphere revealed an increase in PlGF in both hMSC groups, but a greater increase in the PlGF-hMSC group. These data support the hypothesis that PlGF contributes to neuroprotection and angiogenesis in cerebral ischaemia, and cellular delivery of PlGF to the brain can be achieved by intravenous delivery of hMSCs. © The Author (2006). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved.
  • Yukinori Akiyama, Jun Ikeda, Yukihiro Ibayashi, Tadashi Nonaka, Yasufumi Asai, Kiyohiro Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 46 9 446 - 449 2006年09月 [査読有り][通常論文]
     
    A 38-year-old man presented with a dysplastic cerebellar gangliocytoma associated with a paraspinal arteriovenous fistula (AVF) at the upper cervical portion. Neuroimaging examination indicated the diagnosis of Lhermitte-Duclos disease. The patient was treated by embolization of the AVF followed by partial resection of the cerebellar tumor. Genetic examination showed a mutation of the phosphatase and tensin homolog deleted on chromosome ten (PTEN) gene. Histological examination confirmed the diagnosis. Lhermitte-Duclos disease is a rare dysplastic gangliocytoma manifesting as a slowly growing mass in the cerebellum and is usually asymptomatic. This case of Lhermitte-Duclos disease associated with paraspinal AVF and mutation of the PTEN gene suggests a relationship between Lhermitte-Duclos disease and Cowden disease.
  • Takeshi Mikami, Yoshihiro Minamida, Tetsuo Himi, Kiyohiro Houkin
    NEUROLOGICAL SURGERY 34 9 933 - 937 2006年09月 [査読有り][通常論文]
     
    A case of inner ear anomalies associated with recurrent meningitis found in a 66-year-old female is reported. The patient had had 2 episodes of meningitis in the previous one year period. Multidetector-row CT of the temporal bone and three-demensional fast imaging employing steady-state acquisition MRI revealed dysplasia of the bony labyrinth and enlarged fundus of the internal auditory canal (IAC). During surgical treatment, we confirmed the cerebrospinal fluid (CSF) leakage through a bony defect in the stapedial footplate. The inner ear was obliterated and contained small pieces of temporal fascia. A meningitis or CSF leakage due to innar ear malformation is extreamly rear condition for neurosurgeon. Though we always have to take it into condideration as a cause of CSF leakage.
  • 山下 太郎, 三上 毅, 南田 善弘, 馬場 雄大, 宝金 清博
    Neurological Surgery 34 8 833 - 837 (株)医学書院 2006年08月 [査読無し][通常論文]
     
    66歳女.頭痛,視力障害が出現した.視力は右0.1(0.5),左0.5(0.8)で,ゴールドマン視野検査で右眼外側下1/4盲を,眼底検査では右側の視神経萎縮を認めた.CTでは右前床突起内に等吸収の腫瘤を認めたが,骨肥厚や脱灰像はなく,病変のCT値は健側の脂肪髄よりも高値であった.MRIで同部位はT1,T2強調像で高信号を示し,造影剤により増強され,拡散強調像では高信号を呈した.視力障害が進行したため,右前頭側頭開頭にて腫瘍切除術を行った.前床突起表面は皮質骨が菲薄化して内部の腫瘍が透見され,drillingにて暗赤色の腫瘤性病変を認めた.腫瘍は前床突起に限局しており,皮膜の形成や腫瘍へのfeeder,drainerもなかった.腫瘍を前床突起ごと全摘出し,視束管開放を行った.術後経過は良好で,頭痛は消失したが,視力視野は改善しなかった.病理組織所見で骨梁間に拡張した腔を形成する血管の増生と脂肪の増生を認め,cavernous hemangiomaと診断した
  • 中山 若樹, 黒田 敏, 石川 達哉, 志賀 哲, 宝金 清博, 玉木 長良, 岩崎 喜信
    脳循環代謝 18 2 135 - 135 (一社)日本脳循環代謝学会 2006年08月 [査読無し][通常論文]
  • T. Honma, O. Honmou, S. Iihoshi, K. Harada, K. Houkin, H. Hamada, J. D. Kocsis
    EXPERIMENTAL NEUROLOGY 199 1 56 - 66 2006年05月 [査読有り][通常論文]
     
    Intravenous infusion of bone marrow cells has demonstrated therapeutic efficacy in animal models of cerebral ischemia and spinal cord injury. We intravenously delivered human mesenchymal stem cells (SH2+, SH3+, CD34(-), and CD45(-)) immortalized with a human-telomerase gene (hTERT-MSCs) and transfected with eGFP or LacZ into rats 12 h after induction of transient middle cerebral artery occlusion (MCAO), to study their potential therapeutic benefit. hTERT-MSCs were delivered at 12 It after lesion induction. Lesion size was assessed using MR imaging and spectroscopy, and histological methods. Functional outcome was assessed using the Morris water maze and a treadmill test. Intravenous delivery of hTERT-MSCs reduced lesion volume and the magnitude of the reduction and functional improvement was positively correlated with the number of cells injected. The reduction of lesion size could be assessed in vivo with MRI and MRS and was correlated with subsequent histological examination of the brain. This work demonstrates that highly purified hTERT-MSCs reduce cerebral infarction volume and improve functional outcome. (c) 2005 Elsevier Inc. All rights reserved.
  • R Nanba, S Kuroda, M Tada, T Ishikawa, K Houkin, Y Iwasaki
    CHILDS NERVOUS SYSTEM 22 3 258 - 262 2006年03月 [査読有り][通常論文]
     
    Objects: This study aims to clarify the genetic background of moyamoya disease by comparing clinical features between familial and sporadic cases to reveal the responsible genes for familial moyamoya disease. Methods: This study included 155 Japanese patients with moyamoya disease, which included 24 familial cases (10 family pedigrees) and 131 sporadic cases. Clinical features were compared between the familial and sporadic cases. Results and conclusions: A female preponderance was significantly more prominent in the familial than in the sporadic group (P=0.0421). Mean age at onset was significantly lower in familial than in sporadic cases (P=0.004). In eight parent-offspring pairs, mean age at onset was significantly lower in the second than in the first generation (P < 0.0001). These results suggest that familial moyamoya disease is associated with genetic anticipation and female predominance and that a genetic analysis study focused on expanded triplet repeats may clarify the pathogenesis of the disease.
  • W Zheng, M Wanibuchi, T Onda, H Liu, Koyanagi, I, K Fujimori, K Houkin
    CHILDS NERVOUS SYSTEM 22 3 274 - 278 2006年03月 [査読有り][通常論文]
     
    Case report: We describe a 15-year-old girl with moyamoya disease whose initial manifestation was chorea-like involuntary movements. T2-weighted magnetic resonance imaging showed high signal intensity lesions in the left frontal lobe, right parieto-occipital lobes, and frontal subcortical white matter. Single-photon emission computed tomography (SPECT) showed diffuse hypoperfusion of the whole brain. Bilateral direct and indirect cerebrovascular bypass surgeries were performed. Chorea disappeared 2 days after the surgery. Follow-up SPECT demonstrated increased cerebral perfusion in the bilateral frontal, temporal, and parietal regions. Conclusions: Chorea accompanied with moyamoya disease can be properly managed by revascularization surgery. Moyamoya disease should be remembered as being one of the differential diagnoses of chorea, which is treatable by surgery.
  • S Kuroda, T Shiga, K Houkin, T Ishikawa, C Katoh, N Tamaki, Y Iwasaki
    STROKE 37 2 393 - 398 2006年02月 [査読有り][通常論文]
     
    Background and Purpose-It is still unclear that impaired cerebrovascular reactivity (CVR) to acetazolamide is comparable to elevated oxygen extraction fraction (OEF) on positron emission tomography (PET) in patients with occlusive carotid diseases. Therefore, in this study, the authors aimed to clarify whether OEF is elevated in all patients with reduced cerebral blood flow (CBF) and CVR (type 3) on single photon emission computed tomography (SPECT), and, if not, to specify the underlying pathophysiology of type 3 but normal OEF. Methods-This study included 46 patients who had decreased CBF and CVR on N-isopropyl-p-I-123-iodoamphetamine SPECT in the ipsilateral middle cerebral artery area attributable to occlusive carotid diseases. Hemodynamic and metabolism parameters were determined in all patients by O-15-gas PET, and neuronal integrity was evaluated in 19 patients using C-11-flumazenil (FMZ) PET. Results-OEF was significantly elevated in 20 (43.5%) of 46 type 3 patients. Another 26 type 3 patients had normal OEF. Regression analysis showed that OEF significantly correlated with cerebral metabolic rate for oxygen and C-11-FMZ binding potential but not with other parameters. Subcortical infarction had no significant effect on OEF values. Conclusions-The results strongly suggest that type 3 patients with reduced CBF and CVR may be divided into 2 pathophysiologically different subgroups: misery perfusion attributable to hemodynamic compromise and matched hypometabolism attributable to incomplete infarction. Type 3 but normal OEF may represent a transition stage from misery perfusion to matched hypometabolism.
  • M Ohsaka, K Houkin, M Takigami, Koyanagi, I
    PEDIATRIC NEUROLOGY 34 2 160 - 163 2006年02月 [査読有り][通常論文]
     
    An extremely rare case of acute necrotizing encephalopathy caused by human herpesvirus-6 variant type B infection is reported. The patient, a 14-month-old previously healthy female, presented with high fever and generalized tonic convulsion followed by rapid deterioration of consciousness. On the second day of the illness, the protein level of the cerebrospinal fluid increased without pleocytosis. On the third day, magnetic resonance images demonstrated symmetric, abnormal signal intensity lesions in the bilateral thalamus, cerebellum, and brainstem. On the fourth day, characteristic maculopapular rash of exanthema subitum appeared on the trunk. Human herpesvirus-6 deoxyribonucleic acid was detected by the polymerase chain reaction in the serum, and immunoglobulin G and inummoglobulin M of serum human herpesvirus-6 were positive. On the twelfth day of illness, the patient died as a result of severe brain damage. Acute necrotizing encephalopathy should be included in the differential diagnosis when examining infants and young children with fulminating consciousness disturbance and intractable convulsion. In addition, as a causative virus, human herpesvirus-6 has to be considered at the pre-eruptive stage of exanthema subitum. Magnetic resonance images are useful because they reveal the characteristic distribution of lesions specific to acute necrotizing encephalopathy. (c) 2006 by Elsevier Inc. All rights reserved.
  • H Kano, K Houkin, K Harada, Koyanagi, I, S Nara, Y Itou, H Imaizumi, Y Asai, M Saitou
    NEUROSURGICAL REVIEW 29 1 88 - 92 2006年01月 [査読有り][通常論文]
     
    Neuronal cell injury after global cerebral ischemic insult is not well understood in humans. We performed serial examination of diffusion-weighted imaging and magnetic resonance spectroscopy in three patients after cardiopulmonary resuscitation. The presence of the signal for lactate in magnetic resonance spectroscopy in the acute stage after cardiopulmonary resuscitation was closely correlated to irreversible damage. In addition, high intensity in diffusion-weighted magnetic resonance image in the acute stage also predicted a poor outcome. Lesions that were positive for these factors in the acute stage led to serious brain damage in the subacute and chronic stages. The results indicated that after cardiopulmonary resuscitation, diffusion-weighted magnetic resonance imaging and magnetic resonance spectroscopy is an extremely useful modality to estimate the prognosis of patients, which is not always easy using conventional methods.
  • 南田善弘, 三上毅, 寳金清博, 村上弦
    日本内分泌学会雑誌 82 Suppl. (June) 2006年
  • 本望修, 寳金清博
    日本臨床 64 2006年
  • Misuzu Ohsaka, Tadashi Nonaka, Shinichi Oka, Yoshihiro Minamida, Takeshi Mikami, Kiyohiro Houkin
    Brain and Nerve 58 1 57 - 61 2006年01月 [査読有り][通常論文]
     
    A 33-year-old female who had been on a steroid treatment for the past 14 years due to systemic lupus erythematosus (SLE) visited our hospital complaining of mild headache. No neurological deficit and no positive serologic tests for lupus anticoagulants (LAC) and anticardiolipin antibodies (aCL) were noted. Only a mild inflammatory change was observed on routine hematological examination. On neuroradiological examination, MRI revealed thickened falx cerebri and tentorium cerebelli, and an empty delta sign. These findings were suggestive of sinus thrombosis of superior sagittal sinus (SSS). Angiograms clearly demonstrated occlusion of the posterior part of superior sagittal sinus and transeverse sinus (TS). Conservative treatment was chosen because of no evidence of intracranial hypertension. There was no deterioration in her general and neurological status during her hospital stay and she was discharged. Long-standing vasculitis and pachymeningitis related to lupus erythematosus might be the probable cause of the sinus thrombosis in this case.
  • T Nonaka, S Oka, S Miyata, T Baba, T Mikami, K Houkin
    INTERVENTIONAL NEURORADIOLOGY 12 205 - 210 2006年01月 [査読有り][通常論文]
     
    This study is performed to investigate risk factors of hypotension in response to elective carotid stenting. Forty-four lesions of 40 consecutive patients (mean age 70.4 +/- 8.2 years) were retrospectively analyzed. Easy Wall stent was applied in 15 lesions and SMART stent in 29 lesions. We investigated correlations between the occurrence rate of postoperative hypotension below 90 mmHg and persisting over three hours and findings of preoperative angiograms, ultrasonograms and clinical characteristics. Postprocedural hypotension occurred in 19 patients (47.5%) and medical treatment (intravenous administration of catecholamines) was required in eleven patients (27.5%). Although there was no permanent neurological deficits related with postprocedural hypotension, transient neurological deficits were found in three patients. Risk factors of prolonged postprocedural hypotension were statistically analyzed. On angiographic characteristics; 1) distance between the carotid bifurcation and the lesion with maximum stenosis (<= 10 mm vs. > 10 mm: p = 0. 031), 2) type of stenosis (eccentric vs. concentric: p = 0. 014) On ultrasonographic characteristics; 1) calcifications at the carotid bifurcation (present vs. absent: p<0.001). Other variables, including age and degree of stenosis, were not associated with postprocedural hypotension after carotid stenting. These angiographic and ultrasonographic variables can be used to identify patients at risk for postprocedural hypotension after carotid stenting. Such identification may help in selection of patients who will benefit from appropriate pharmacological treatment.
  • K. Houkin, N. Nakayama, T. Nonaka, I. Koyanagi
    Journal of International Medical Research 34 1 65 - 72 2006年 [査読有り][通常論文]
     
    In this study, the effect of sarpogrelate hydrochloride, a 5-hydroxytryptamine2A receptor antagonist, on platelet aggregation at the site of injured carotid artery endothelium was examined. The rat common carotid artery was clamped for 30 min to induce endothelial injury. Sarpogrelate hydrochloride was administered before and after the injury, and the effects were compared with those in rats receiving sham operation only and those receiving clipping injury but no sarpogrelate hydrochloride. The animals were killed 24 h after the procedure. The common carotid artery was examined by scanning electron microscopy and stained immunochemically for factor VIII. Sarpogrelate hydrochloride treatment was associated with reduced aggregation of platelets on electron microscopy and lower expression of factor VIII at the injured intima. Sarpogrelate hydrochloride has an inhibitory effect on platelet aggregation at the intima in the acute stage after injury, suggesting that this drug may be used to prevent early ischaemic complications after surgical or endovascular arterial intervention. Copyright © 2006 Cambridge Medical Publications.
  • T Baba, Y Minamida, T Mikami, Koyanagi, I, K Houkin
    JOURNAL OF NEUROSURGERY 103 5 917 - 919 2005年11月 [査読有り][通常論文]
     
    The authors report on the case of a 14-year-old boy who presented with bilateral visual impairment due to optic canal stenosis caused by hyperplasia of the bone marrow arising from anemia. The patient had hereditary hemolytic anemia with unstable hemoglobin of the Christchurch type. This congenital form of anemia caused hyperplasia of the bone marrow as well as hyperostosis of the entire calvarial bone, which in turn led to optic canal stenosis. The patient underwent surgical decompression of the optic canal, resulting in significant improvement in visual acuity. Pathological findings in the calvarial bone indicated hypertrophic bone mar-row with no other specific features such as neoplastic pattern or fibrous dysplasia. With the exception of objective hearing impairment, no other significant cranial neuropathy has been detected thus far. On reviewing the published literature, this case was found to be the first in which hyperostosis due to congenital anemia resulted in symptomatic entrapment neuropathy of the optic nerve. The authors concluded that surgical decompression effectively improves visual acuity.
  • S Kuroda, T Ishikawa, K Houkin, R Nanba, M Hokari, Y Iwasaki
    STROKE 36 10 2148 - 2153 2005年10月 [査読有り][通常論文]
     
    Background and Purpose - The progression of occlusive lesions in the major intracranial arteries was believed to be very rare in adult patients with moyamoya disease. The present study aims to clarify the incidence and clinical features of disease progression in adult moyamoya disease. Methods - For the past 15 years, 120 adult Japanese patients were diagnosed with moyamoya disease. Of these, 63 patients were enrolled in this historical prospective cohort study on a total of 86 nonoperated hemispheres. All were followed up with a mean period of 73.6 months. MRI and magnetic resonance angiography were repeated every 6 to 12 months, and cerebral angiography was performed when disease progression was suspected on MRI and magnetic resonance angiography. Results - Disease progression occurred in 15 of 86 nonoperated hemispheres (17.4% per hemisphere) or in 15 of 63 patients (23.8% per patient) during the follow-up period. Occlusive arterial lesions progressed in both anterior and posterior circulations, in both symptomatic and asymptomatic patients, and in both bilateral and unilateral types. Eight of 15 patients developed ischemic or hemorrhagic events in relation to disease progression. Multivariate analysis revealed that the odds ratio conferred by a male patient was 0.20 (95% CI, 0.04 to 0.97). Conclusions - The incidence of disease progression in adult moyamoya disease is much higher than recognized before, and female patients may be at higher risk for it than male patients. Careful follow-up would be essential to prevent additional stroke occurrence in medically treated adult patients with moyamoya disease, even if they are asymptomatic or are diagnosed as having unilateral moyamoya disease.
  • T Mikami, Y Minamida, T Yamaki, Koyanagi, I, T Nonaka, K Houkin
    NEUROSURGICAL REVIEW 28 4 261 - 266 2005年10月 [査読有り][通常論文]
     
    Steady-state free precession is widely used for ultra-fast cardiac or abdominal imaging. The purpose of this work was to assess fast imaging employing steady-state acquisition (FIESTA) and to evaluate its efficacy for depiction of the cranial nerve affected by the tumor. Twenty-three consecutive patients with posterior fossa tumors underwent FIESTA sequence after contrast agent administration, and then displacement of the cranial nerve was evaluated. The 23 patients with posterior fossa tumor consisted of 12 schwannomas, eight meningiomas, and three cases of epidermoid. Except in the cases of epidermoid, intensity of all tumors increased on FIESTA imaging of the contrast enhancement. In the schwannoma cases, visualization of the nerve became poorer as the tumor increased in size. In cases of encapsulated meningioma, all the cranial nerves of the posterior fossa were depicted regardless of location. The ability to depict the nerves was also significantly higher in meningioma patients than in schwannoma patients (P < 0.05). In cases of epidermoid, extension of the tumors was depicted clearly. Although the FIESTA sequence offers similar contrast to other heavily T2-weighted sequences, it facilitated a superior assessment of the effect of tumors on cranial nerve anatomy. FIESTA sequence was useful for preoperative simulations of posterior fossa tumors.
  • K Houkin, T Nonaka, S Oka, Koyanagi, I
    NEUROLOGIA MEDICO-CHIRURGICA 45 9 448 - 453 2005年09月 [査読有り][通常論文]
     
    Social demand for the disclosure of medical information is increasing, especially the treatment for unruptured intracranial aneurysms. This study investigated to what extent information on the treatment for unruptured intracranial aneurysms is disclosed on websites in Japan. We surveyed 1225 institutions authorized by The Japan Neurosurgical Society. The following factors were analyzed: percentage of institutions with websites, disclosure of number of surgeries, and disclosure of outcome of treatment for ruptured and unruptured intracranial aneurysms. Of the 1225 institutions surveyed, 1097 (89.6 %) had their own websites. The total number of websites was 1262 since some institutions have several homepages in different websites. The annual number of surgeries was shown in 274 of the 1225 institutions (22.4%). The outcome of treatment for ruptured intracranial aneurysms was disclosed in 104 of the 1225 institutions (8.5%). The outcome of treatment for unruptured intracranial aneurysms was shown in only 32 of the 1225 institutions (2.6%). Disclosure of outcome of treatment for unruptured intracranial aneurysms on websites is not common. To improve disclosure of the outcome on websites, guidelines should be established.
  • T Mikami, N Masauzi, J Niwa, K Houkin
    NEUROSURGERY 57 3 595 - 595 2005年09月 [査読有り][通常論文]
     
    OBJECTIVE AND IMPORTANCE: Acquired factor VIII inhibitors in patients without hemophilia is rare event characterized by severe bleeding as a result of antibody against factor VIII. We describe a patient who developed subcutaneous and epidural bleeding after the clipping of an unruptured aneurysm. CLINICAL PRESENTATION: A 57-year-old man suddenly developed coagulopathy after undergoing a neurosurgical operation. Coagulation tests showed a prolongation of activated partial thromboplastin time and a marked reduction in factor VIII activity. Measurement of the factor VIII inhibitor level revealed 2 Bethesda units/ml. INTERVENTION: The hematoma was removed surgically, and bleeding was controlled with recombinant factor VIII. The patients then received prednisolone (80 mg/d) and cyclosporine. The laboratory tests normalized within 8 weeks. In the 5 years since cessation of corticosteroids, the clinical and laboratory courses of the patient have been uneventful. CONCLUSION: Autoimmune factor VIII inhibitors associated with a neurosurgical procedure are rare. Our case supports an association between surgery and the triggering of factor VIII inhibitors. This complication was probably the result of the surgical procedure or anesthetic agents or other drugs administrated during the perioperative period.
  • Tadashi Nonaka, Shinichi Oka, Kei Miyata, Takeshi Mikami, Izumi Koyanagi, Kiyohiro Houkin, Kazuhisa Yoshifuji, Toshio Imaizumi
    Neurosurgery 57 3 472 - 476 2005年09月 [査読有り][通常論文]
     
    OBJECTIVE: Stent placement for the treatment of carotid artery stenosis may cause hemodynamic instability that induces ischemic complications for patients with bilateral carotid lesions. We carried out this study to define predictors of persistent hypotension after carotid stenting. METHODS: Thirty-three lesions in 31 consecutive patients (mean age, 69.2 ± 8.6 yr) who underwent stent deployment for carotid stenosis were studied. Fourteen lesions were treated with Easy Wall stents (Boston Scientific, Tokyo, Japan) and 19 lesions were treated with Smart stents (Johnson & Johnson, Warren, NJ). We reviewed preoperative angiograms and ultrasonograms of the carotid artery and analyzed the predictive factors of postprocedural prolonged hypotension (systolic blood pressure < 90 mm Hg and periods > 3 h). RESULTS: Postprocedural hypotension was observed in 14 lesions (42.4%), of which medical treatment was necessary in seven (21.2%). A distance between carotid bifurcation and maximum stenotic lesion (≤10 mm P = 0.0028) and type of stenosis (eccentric P = 0.0287) on angiogram and fibrous plaque morphological features (P = 0.0008) and calcifications at carotid bifurcation (P = 0.0004) on ultrasonograms were determined to be independent risk factors of postprocedural hypotension. We introduced a scoring system for predicting prolonged hypotension that included factors such as the distance from carotid bifurcation to maximum stenotic lesion (≤10 mm), type of stenosis (eccentric), plaque morphological features (echogenic), and calcification at carotid bifurcation. The score was determined by adding one point for each of these factors. Three points or more on this score strongly suggested a high risk of prolonged hypotension. CONCLUSION: Our scoring system, which includes angiographic and ultrasonographic findings, may be a good index for the prediction of prolonged hypotension after carotid stenting and may contribute to the reduction of periprocedural ischemic events.
  • T Nonaka, S Oka, K Miyata, T Mikami, L Koyanagi, K Houkin, K Yoshifuji, T Imaizumi
    NEUROSURGERY 57 3 472 - 476 2005年09月 [査読有り][通常論文]
     
    OBJECTIVE: Stent placement for the treatment of carotid artery stenosis may cause hemodynamic instability that induces ischemic complications for patients with bilateral carotid lesions. We carried out this study to define predictors of persistent hypotension after carotid stenting. METHODS: Thirty-three lesions in 31 consecutive patients (mean age, 69.2 +/- 8.6 yr) who underwent stent deployment for carotid stenosis were studied. Fourteen lesions were treated with Easy Wall stents (Boston Scientific, Tokyo, Japan) and 19 lesions were treated with Smart stents (Johnson & Johnson, Warren, NJ). We reviewed preoperative angiograms and ultrasonograms of,the carotid artery and analyzed the predictive factors of postprocedural prolonged hypotension (systolic blood pressure < 90 mm Hg and periods > 3 h). RESULTS: Postprocedural hypotension was observed in 14 lesions (42.4%), of which medical treatment was necessary in seven (21.2%). A distance between carotid bifurcation and maximum stenotic lesion (<= 10 mm; P = 0.0028) and type of stenosis (eccentric; P = 0.0287) on angiogram and fibrous plaque morphological features (P = 0.0008) and calcifications at carotid bifurcation (P = 0.0004) on ultrasonograms were determined to be independent risk factors of postprocedural hypotension. We introduced a scoring system for predicting prolonged hypotension that included factors such as the distance from carotid bifurcation to maximum stenotic lesion (<= 10 mm), type of stenosis (eccentric), plaque morphological features (echogenic), and calcification at carotid bifurcation. The score was determined by adding one point for each of these factors. Three points or more on this score strongly suggested a high risk of prolonged hypotension. CONCLUSION: Our scoring system, which includes angiographic and ultrasonographic findings, may be a good index for the prediction of prolonged hypotension after carotid stenting and may contribute to the reduction of periprocedural ischemic events.
  • H Liu, T Yamaki, S Oka, Koyanagi, I, K Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 45 8 418 - 422 2005年08月 [査読有り][通常論文]
     
    A 54-year-old man, with a past history of renal cell carcinoma, presented with bitemporal visual field defect, hyponatremia, and diabetes insipidus. Endocrinological examination revealed panhypopituitarism. Computed tomography and magnetic resonance imaging showed an intrasellar mass with suprasellar extension. The initial radiological impression was pituitary adenoma. The tumor was decompressed via the transsphenoidal route. Histological examination revealed metastatic renal cell carcinoma. The clinical characteristics of metastatic pituitary carcinoma appear to be panhypopituitarism, and neuroimaging findings of strong enhancement of the tumor and bony destruction without marked sellar enlargement.
  • Y Minamida, T Mikami, K Hashi, K Houkin
    JOURNAL OF NEUROSURGERY 103 2 224 - 232 2005年08月 [査読有り][通常論文]
     
    Object. The authors performed a retrospective analysis of a consecutive series of craniopharyngiomas and their recurrences, which were managed with surgery alone. Methods. In the past 20 years, 37 consecutive patients with craniopharyngiomas underwent surgery without adjuvant radiotherapy. During that period there was a consistent strategy that surgical management was the first choice of treatment whenever possible. Of these 37 patients, 11 experienced tumor recurrence (29.7%) during the mean follow-up period of 11.1 years. Of these 11 patients, seven experienced recurrence after neuroimaging-confirmed total removal, and four patients experienced recurrence after partial or incomplete removal. In these 11 patients, surgical removal was performed 17 times. Using a proper surgical approach (mainly a basal interhemispheric approach) and meticulous microsurgical techniques, total removal of the recurrent tumor was achieved in nine surgeries (52.9). The mortality and morbidity rates associated with these 17 surgeries were 0% and 9.1%, respectively. In most cases, visual function was preserved or improved and intellectual performance was also preserved. Conclusions. Recurrence of craniopharyngioma can be safely managed by using meticulous contemporary microsurgical techniques without additional radiotherapy. The role of surgery and adjuvant radiotherapy for craniopharyngiomas may vary in the future, depending on innovations in treatment and technology. Nevertheless, surgery can be still a major therapeutic option in the management of recurrent craniopharyngiomas.
  • Koyanagi, I, Y Iwasaki, K Hida, K Houkin
    SURGICAL NEUROLOGY 63 4 350 - 355 2005年04月 [査読有り][通常論文]
     
    Background: Syringomyelia is a common intramedullary lesion associated with spinal arachnoiditis and obstruction of the foramen magnum such as in Chiari's malformation. Disturbance of cerebrospinal fluid flow around the spinal cord has an important role in the development of syringomyelia due to spinal arachnoiditis; however, the exact mechanisms have not been clarified. The purpose of this retrospective study is to understand the clinical features and pathomechanisms of syringomyelia secondary to spinal arachnoiditis and to provide the current choice of surgical treatment in this difficult clinical entity. Methods: Clinical and radiological findings in 15 patients with syringomyelia associated with spinal arachnoiditis who underwent surgical treatment in our institutes between 1982 and 2000 were reviewed. All patients presented with paraparesis or tetraparesis on admission. Results: Magnetic resonance imaging (MRI) or computed tomography-myelography revealed that the syrinx predominantly existed at the thoracic levels. Five patients showed complete block of the thoracic subarachnoid space by conventional myelography. T2-weighted MRI showed diffuse intramedullary hyperintensity at the level of arachnoiditis. As the first surgical treatment, 10 patients underwent syringo-peritoneal shunt placement. Three patients were treated with a syringo-subarachnoid shunt, and 2 patients were treated with a ventriculoperitoneal shunt. Eight patients required further shunting operations for syringomyelia 2 months to 12 years after the first surgery. Neurologic improvement was obtained in 9 patients (60%) with decreased size of the syrinx. One patient remained stable; 5 patients showed gradual deterioration. Conclusions: The syrinx originated from the thoracic levels where severe adhesion of the subarachnoid space was present. The mechanisms of syrinx formation may be based on the increased interstitial fluid in the spinal cord. Shunting procedures were effective in some population of the patients. Decompression procedures of the spinal subarachnoid space may be an alternative primary surgical treatment except for patients with longitudinally extensive arachnoiditis. (c) 2005 Elsevier Inc. All rights reserved.
  • T Mikami, A Takahashi, K Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 45 4 201 - 204 2005年04月 [査読有り][通常論文]
     
    47-year-old man presented with carotid rete mirabile manifesting as subarachnoid hemorrhage (SAH). Computed tomography showed SAH, and angiography disclosed an abnormal vascular network around the petrous and cavernous portions of the internal carotid artery. Single photon emission computed tomography (SPECT) with technetium-99m methyl cysteinate dimer revealed reduced regional cerebral blood How (CBF). Twelve months later, he was leading a normal life without neurological problems, hemorrhage, or ischemic manifestations. SPECT with iodine-123 N-isopropyl-p-iodoamphetamine and the acetazolamide challenge test showed the CBF had normalized.
  • 野村 達史, 本望 修, 原田 邦明, 劉 赫, 寶金 清博, 中村 公則, 濱田 洋文
    脳卒中 27 1 203 - 203 (一社)日本脳卒中学会 2005年04月
  • H Hamada, M Kobune, K Nakamura, Y Kawano, K Kato, O Honmou, K Houkin, T Matsunaga, YN Niitsu
    CANCER SCIENCE 96 3 149 - 156 2005年03月 [査読有り][通常論文]
     
    We developed human mesenchymal stem cell (MSC) lines that could differentiate into various tissue cells including bone, neural cells, bone marrow (BM) stromal cells supporting the growth of hematopoietic stem cell (HSC), and so-called 'tumor stromal cells' mixing with tumor cells. We investigated the applicability of MSC as therapeutic cell transplanting reagents (cytoreagents). Telomerized human BM derived stromal cells exhibited a prolonged lifespan and supported the growth of hematopoietic clonogenic cells. The gene transfer of Indian hedgehog (Ihh) remarkably enhanced the HSC expansion supported by the human BM stromal cells. Gene-modified MSC are useful as therapeutic tools for brain tissue damage (e.g. brain infarction) and malignant brain neoplasms. MSC transplantation protected the brain tissue from acute ischemic damage in the midcerebral artery occlusion (MCAO) animal model. Brain-derived neurotrophic factor (BDNF)-gene transduction further enhanced the protective efficacy against the ischemic damage. MSC possessed excellent migratory ability and exerted inhibitory effects on the proliferation of glioma cells. Gene-modification of MSC with therapeutic cytokines clearly augmented the antitumor effect and prolonged the survival of tumor-bearing animals. Gene therapy employing MSC as a tissue-protecting and targeting cytoreagent would be a promising approach.
  • H Shichinohe, S Kuroda, T Asano, S Ushikoshi, T Ishikawa, K Houkin, T Murashita, Y Iwasaki
    NEUROLOGICAL SURGERY 33 2 149 - 153 2005年02月 [査読有り][通常論文]
     
    Recently, there are increasing numbers of patients with occlusive carotid artery disease and coronary artery disease. Simultaneous or two-staged surgery for both lesions has been recommended for these patients to reduce the incidence of perioperative complications. However, therapeutic options for the patients with bilateral carotid artery stenosis and coronary artery disease have not been established. In this report, we describe two patients who a successfully underwent carotid endarterectomy (CEA) and carotid artery stenting (CAS) on each carotid artery in parallel with coronary artery bypass grafts (CABG). A 49-year-old male with severe stenosis of the bilateral internal carotid artery (ICA) and heart failure underwent CAS on the right side. Next day, he successfully under-went CABG and CEA on the left side at the same time. A 62-year-old male with severe stenosis of the bilateral ICA and coronary artery disease underwent CAS on the right side and CEA on the left side with an interval of 7 days. Subsequently, CABG was performed uneventfully. No perioperative complication occurred in either patient. The results suggest that combination therapy of CAS and CEA would be a valuable option for patients with complex carotid/coronary artery diseases.
  • K Houkin, N Nakayama, S Kuroda, T Nonaka, T Shonai, T Yoshimoto
    CEREBROVASCULAR DISEASES 20 5 347 - 354 2005年 [査読有り][通常論文]
     
    Background: Magnetic resonance angiography (MRA) has been acknowledged as a noninvasive diagnostic modality for moyamoya disease. However, in terms of staging of moyamoya disease, conventional angiography is still the gold standard. Therefore, the purpose of this study was to establish MRA grades for moyamoya disease as an alternative to conventional angiography. Methods: Twenty-two patients ( 44 sides) with moyamoya disease diagnosed by conventional angiography were evaluated by MRA during the past 5 years. MRA scores were assigned based on the severity of occlusive changes of the internal carotid artery, the horizontal portion of the middle cerebral artery, the anterior and the posterior cerebral arteries and the signals of the distal branches of these arteries. Total points ranged from 0 ( normal) to 10 ( most severe). Results: MRA scores ( 0 - 10) were significantly consistent with the conventional angiographic staging. Four grades based on this novel MRA scores correlated well with Suzuki's stages, with high sensitivity and specificity. Conclusions: These novel MRA grades can be a reliable alternative to conventional staging. By employing these novel MRA grades, the use of conventional angiography can be avoided for the purpose of evaluation of the stages of moyamoya disease. Copyright (C) 2005 S. Karger AG, Basel.
  • K Kurozumi, K Nakamura, T Tamiya, Y Kawano, K Ishii, M Kobune, S Hirai, H Uchida, K Sasaki, Y Ito, K Kato, O Honmou, K Houkin, Date, I, H Hamada
    MOLECULAR THERAPY 11 1 96 - 104 2005年01月 [査読有り][通常論文]
     
    Mesenchymal stem cells (MSC) were reported to ameliorate functional deficits after stroke in rats, with some of this improvement possibly resulting from the action of cytokines secreted by these cells. To enhance such cytokine effects, we previously transfected the telomerized human MSC with the BDNF gene using a fiber-mutant adenovirus vector and reported that such treatment contributed to improved ischemic recovery in a rat transient middle cerebral artery occlusion (MCAO) model. In the present study, we investigated whether other cytokines in addition to BDNF, i.e., GDNF, CNTF, or NT3, might have a similar or greater effect in this model. Rats that received MSC-BDNF (P < 0.05) or MSC-GDNF (P < 0.05) showed significantly more functional recovery as demonstrated by improved behavioral test results and reduced ischemic damage on MRI than did control rats 7 and 14 days following MCAO. On the other hand, rats that received MSC-CNTF or MSC-NT3 showed neither functional recovery nor ischemic damage reduction compared to control rats. Thus, MSC transfected with the BDNF or GDNF gene resulted in improved function and reduced ischemic damage in a rat model of MCAO. These data suggest that gene-modified cell therapy may be a useful approach for the treatment of stroke.
  • H Yasuda, S Kuroda, S Ushikoshi, T Kato, K Houkin, Y Iwasaki, H Abe
    NEUROLOGIA MEDICO-CHIRURGICA 45 1 37 - 40 2005年01月 [査読有り][通常論文]
     
    A 67-year-old male presented with a rare pseudoaneurysm caused by infection after carotid endarterectomy (CEA) performed for stenosis of the left internal carotid artery (ICA). Wound infection and recurrent bleeding from the operated ICA developed 1 month after surgery. Serial angiography showed that the post-CEA pseudoaneurysm gradually increased in size. The carotid balloon occlusion test revealed that the patient could not tolerate permanent ICA occlusion because of poorly developed collaterals. Direct surgical exposure of the aneurysm was impossible due to tight adhesion of the surrounding tissue, so common carotid to middle cerebral artery bypass using a radial artery graft was performed followed by ligation of the distal common carotid artery. Subsequently, retrograde blood flow from the ICA to the aneurysm was interrupted by embolization of the external carotid artery coil through the facial artery. Combined surgical and endovascular treatment is a therapeutic option for patients with post-CEA pseudoaneurysm, if either direct or endovascular surgery is unfeasible.
  • R Nanba, M Tada, S Kuroda, K Houkin, Y Iwasaki
    CHILDS NERVOUS SYSTEM 21 1 62 - 68 2005年01月 [査読有り][通常論文]
     
    Objects: The pathogenesis of moyamoya disease is still unknown. The present study aimed to find out the responsible genes that are located in the 17q25 locus. Methods: Considering the function, we selected nine genes as candidates from a total of 65 genes identified in the 9-cM region of D17S785 - D17S836 in chromosome 17q25, and performed sequence analysis on the DNA samples obtained from a pedigree of familial moyamoya disease, which showed a complete linkage to the region by a haplotype analysis. Also, we attempted to identify candidate genes that have not been known but might be functionally relevant to the disease among a total of 2,100 expressed sequence tag ( EST) sequences using bioinformatics techniques. Results and conclusion: The sequence analysis could detect no mutation in the nine genes. Nor could we identify a novel candidate gene by the EST analysis. Further studies using alternative approaches are warranted to clarify the pathogenesis of moyamoya disease.
  • T Nomura, O Honmou, K Harada, K Houkin, H Hamada, JD Kocsis
    NEUROSCIENCE 136 1 161 - 169 2005年 [査読有り][通常論文]
     
    I.v. delivery of mesenchymal stem cells prepared from adult bone marrow reduces infarction size and ameliorates functional deficits in rat cerebral ischemia models. Administration of the brain-derived neurotrophic factor to the infarction site has also been demonstrated to be neuroprotective. To test the hypothesis that brain-derived neurotrophic factor contributes to the therapeutic benefits of mesenchymal stem cell delivery, we compared the efficacy of systemic delivery of human mesenchymal stem cells and human mesenchymal stem cells transfected with a fiber-mutant F/RGD adenovirus vector with a brain-derived neurotrophic factor gene (brain-derived neurotrophic factor-human mesenchymal stem cells). A permanent middle cerebral artery occlusion was induced by intraluminal vascular occlusion with a microfilament. Human mesenchymal stem cells and brain-derived neurotrophic factor-human mesenchymal stem cells were i.v. injected into the rats 6 h after middle cerebral artery occlusion. Lesion size was assessed at 6 h, 1, 3 and 7 days using MR imaging, and histological methods. Functional outcome was assessed using the treadmill stress test. Both human mesenchymal stem cells and brain-derived neurotrophic factor-human mesenchymal stem cells reduced lesion volume and elicited functional improvement compared with the control sham group, but the effect was greater in the brain-derived neurotrophic factor-human mesenchymal stem cell group. ELISA analysis of the infarcted hemisphere revealed an increase in brain-derived neurotrophic factor in the human mesenchymal stem cell groups, but a greater increase in the brain-derived neurotrophic factor-human mesenchymal stem cell group. These data support the hypothesis that brain-derived neurotrophic factor contributes to neuroprotection in cerebral ischemia and cellular delivery of brain-derived neurotrophic factor can be achieved by i.v. delivery of human mesenchymal stem cells. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.
  • R Nanba, S Kuroda, T Ishikawa, K Houkin, Y Iwasaki
    STROKE 35 12 2837 - 2842 2004年12月 [査読有り][通常論文]
     
    Background and Purpose-The etiology of moyamoya disease still remains unknown. This study was aimed to explore the role of hepatocyte growth factor (HGF), a strong inducer of angiogenesis, in development of moyamoya disease. Methods-We studied cerebrospinal fluid (CSF) from 39 patients with moyamoya disease (24 children and 15 adults), 6 control patients with cervical spondylosis, and 7 control patients with internal carotid artery occlusion. CSF level of HGF was determined by enzyme-linked immunosorbent assay technique. We also evaluated the distribution of HGF and its cellular receptor c-Met in the carotid fork obtained from 2 patients with moyamoya disease and 2 control patients. Results-CSF level of HGF was 408.2+/-201.6 pg/mL and 443.2+/-193.5 pg/mL in patients with cervical spondylosis and internal carotid artery occlusion, respectively (mean+/-SD). On the other hand, CSF level of HGF was 820.3+/-319.0 pg/mL in patients with moyamoya disease, being significantly higher than those in 2 control groups (P<0.01). Both HGF and c-Met were widely distributed in the media and thickened intima of the carotid fork in patients with moyamoya disease but not in control patients. Conclusions-This study revealed that HGF is densely found in the carotid fork, and its CSF level is markedly elevated in moyamoya disease, suggesting that HGF may be a key protein for pathogenesis of moyamoya disease.
  • S Oka, O Honmou, Y Akiyama, M Sasaki, K Houkin, K Hashi, JD Kocsis
    BRAIN RESEARCH 1030 1 94 - 102 2004年12月 [査読有り][通常論文]
     
    The objective of this study was to establish if neural precursor cells could safely be developed from biopsy of the subventricular zone (SVZ) in the non-human primate (marmoset), and to determine their myelinating potential after autologous transplantation into a demyelinated lesion. Small amounts of tissue were safely collected from the subventricular-subependymal zone of the adult primate brain under ultrasonography without any neurological deficit. Neural precursor cells were isolated and expanded in the presence of mitogen in vitro. The dorsal columns of the adult marmoset spinal cord were demyelinated by X-irradiation and intraspinal injections of ethidium bromide in the center of the radiation field. Cell suspensions of the neural precursors were microinjected through a micropipette into the demyelinated lesion site in the spinal cord. Lesions were histologically examined 3 weeks after transplantation. Light and electron microscopic examination of plastic embedded sections revealed a significant number of myelinating profiles in the transplantation zone; no myelination was observed in control lesions. The myelinated axons had predominantly peripheral patterns of myelination. These results demonstrate that autologous transplantation of neural precursor cells in the adult nonhuman primate can remyelinate demyelinated central nervous system (CNS) axons, thus suggesting the potential utility of such an approach in demyelinating lesions in humans. (C) 2004 Elsevier B.V. All rights reserved.
  • TC Chang, H Shirato, H Aoyama, S Ushikoshi, N Kato, S Kuroda, T Ishikawa, K Houkin, Y Iwasaki, K Miyasaka
    INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 60 3 861 - 870 2004年11月 [査読有り][通常論文]
     
    Purpose: To investigate the appropriateness of the treatment policy of stereotactic irradiation using both hypofractionated stereotactic radiotherapy (HSRT) and stereotactic radiosurgery (SRS) for arteriovenous malformations (AVMs) located in an eloquent region or for large AVMs and using SRS alone for the other AVMs. Methods and Materials: Included in this study were 75 AVMs in 72 patients, with a mean follow-up of 52 months. Of the 75 AVMs, 33 were located in eloquent regions or were >2.5 cm in maximal diameter and were given 25-35 Gy (mean, 32.4 Gy) in four daily fractions at a single isocenter if the patient agreed to prolonged wearing of the stereotactic frame for 5 days. The other 42 AVMs were treated with SRS at a dose of 15-25 Gy (mean, 24.1 Gy) at the isocenter. The 75 AVMs were classified according to the Spetzler-Martin grading system; 21, 23, 28, 2, and 1 AVM were Grade I, II, III, IV, V, and VI, respectively. Results: The overall actuarial rate of obliteration was 43% (95% confidence interval [CI], 30-56%) at 3 years, 72% (95% CI, 58-86%) at 5 years, and 78% (95% CI, 63-93%) at 6 years. The actuarial obliteration rate at 5 years was 79% for the 42 AVMs <2.0 cm and 66% for the 33 AVMs >2 cm. The 5- and 6-year actuarial obliteration rate was 61% (95% Cl, 39-83%) and 71% (95% CI, 47-95%), respectively, after HSRT and 81% (95% CI, 66-96%) and 81% (95% CI, 66-96%), respectively, after SRS; the difference was not statistically significant. Radiation-induced necrosis was observed in 4 subjects in the SRS group and 1 subject in the HSRT group. Cyst formation occurred in 3 patients in the SRS group and no patient in the HSRT group. A permanent symptomatic complication was observed in 3 cases (4.2%), and 1 of the 3 was fatal. All 3 patients were in the SRS group. The annual intracranial hemorrhage rate was 5.5-5.6% for all patients. Conclusion: Our treatment policy using SRS and HSRT was as effective as the policy involving SRS alone. The HSRT schedule was suggested to have a lower frequency of radiation necrosis and cyst formation than the high-dose SRS schedule. The benefit of HSRT compared with lower dose SRS has not yet been determined. (C) 2004 Elsevier Inc.
  • Koyanagi, I, H Imamura, S Fujimoto, K Hida, Y Iwasaki, K Houkin, DJ Maiman
    SURGICAL NEUROLOGY 62 4 286 - 291 2004年10月 [査読有り][通常論文]
     
    BACKGROUND The size of the spinal canal is a factor that contributes to the neurologic deficits associated with cervical ossification of the posterior longitudinal ligament (OPLL). METHODS Bone-window computed tomography (CT) examinations of the cervical spine in 64 patients with cervical OPLL were reviewed. Forty-two patients underwent surgical treatment (anterior decompression: 16 patients, posterior decompression: 26 patients). The remaining 22 patients were managed conservatively. Selection of the surgical approach, anterior or posterior, was based on the longitudinal extent of cord compression. RESULTS The mean developmental size of the spinal canal in the posterior decompression group (10.7 mm at C4) was significantly smaller than the other 2 groups. The spinal canal was narrowed by OPLL to 2.9 to 10.0 mm. The proportion of the patients showing motor deficits of the lower extremities significantly increased when the sagittal canal diameter was narrowed to less than 8 mm. CONCLUSIONS This study demonstrates critical values of CT-determined spinal canal stenosis. Developmental size of the spinal canal and the residual anterior-posterior canal diameters resulting from OPLL spinal cord compression are important factors influencing clinical management and the neurologic state. (C) 2004 Elsevier Inc. All rights reserved.
  • K Houkin, N Nakayama, S Kuroda, T Ishikawa, T Nonaka
    CHILDS NERVOUS SYSTEM 20 10 734 - 741 2004年10月 [査読有り][通常論文]
     
    Object: The dynamic process of neovascularization seen in patients with moyamoya disease who have received bypass surgery has not been sufficiently elucidated. Magnetic resonance angiography (MRA) is expected to disclose this process since it can be conducted frequently because of its non-invasive nature. In this study, we prospectively investigated postoperative neovascularization using repeated MRA. Methods: We studied 14 pediatric patients ( 23 sides) who have received both direct bypass surgery of the superficial temporal artery (STA) to middle cerebral artery and indirect surgery of encephalo-duro-arterio-myo-synangiosis during the past 3 years. MRA was performed using a 1.5-T machine, image sequences of 512 x 256 resolution, and the time-of-flight method. MRA was prospectively performed preoperatively and repeated 1, 3, and 6 months after surgery. Conclusions: A tendency toward a decrease in moyamoya vessels and the development of the STA was observed 1 month after the surgery. Development of the deep temporal artery and the middle meningeal artery was observed 3 months after the surgery. The progression of stenotic change was apparently accelerated after the surgery. A reciprocal relation between neovascularization artificially induced by bypass surgery and moyamoya vessels, which are supposed to compensate for the stenotic change of the internal carotid artery, was disclosed.
  • M Hashimoto, K Ohtsuka, Y Suzuki, Y Minamida, K Houkin
    JOURNAL OF NEURO-OPHTHALMOLOGY 24 3 237 - 239 2004年09月 [査読有り][通常論文]
     
    A 49-year-old man had left superior oblique myokymia for eight years. Magnetic resonance images with enhanced spoiled gradient recalled acquisition in the steady state (SPGR) and flow imaging using steady acquisition (FIESTA) disclosed a branch of the superior cerebellar artery lying on the root exit zone of the left trochlear nerve. Posterior fossa craniotomy confirmed the imaging findings. A Teflon pad was placed between the compressing artery and the trochlear nerve. The patient's superior oblique myokymia has completely resolved with a one-year follow-up. Only one such case has been previously reported. This is the first report to display the imaging findings.
  • ZY Tong, T Yamaki, K Harada, K Houkin
    MAGNETIC RESONANCE IMAGING 22 7 1017 - 1024 2004年09月 [査読有り][通常論文]
     
    Metabolite concentrations in normal adult brains and in gliomas were quantitatively analyzed by in vivo proton magnetic resonance spectroscopy (MRS) using the fully relaxed water signal as an internal standard. Between January 1998 and October 2001, 28 healthy volunteers and 18 patients with gliomas were examined by in vivo proton MRS. Single voxel spectra were acquired using the point-resolved spectroscopic pulse sequence with a 1.5-T scanner (TR/TE/Ave = 3000 ms/30 ms/64). The calculated concentrations of N-acetyl-aspartate (NAA), creatine (Cre), choline (Cho), and water (H2O) in the normal hemispheric white matter were 23.59 +/- 2.62 mM (mean +/- SD), 13.06 +/- 1.8 mM, 4.28 +/- 0.8 mM, and 47280.96 +/- 5414.85 mM, respectively. The metabolite concentrations were not necessarily uniform in different parts of the brain. The concentrations of NAA and Cre decreased in all gliomas (p < 0.001). The NAA/Cho and NAA/H2O ratios can distinguish the normal brain from gliomas, and low-grade astrocytoma from high-grade group (p < 0.001). The concentration of taurine (Tau) in medulloblastomas was 29.64 +/- 5.76 mM. This is the first quantitative analysis of Tau in medulloblastoma in vivo and confirms earlier in vitro findings. (C) 2004 Elsevier Inc. All rights reserved.
  • S Tsushima, M Kai, K Yamada, S Imai, K Houkin, H Kanoh, F Sakane
    JOURNAL OF BIOLOGICAL CHEMISTRY 279 27 28603 - 28613 2004年07月 [査読有り][通常論文]
     
    Nine diacylglycerol kinase (DGK) isozymes have been identified. However, our knowledge of their individual functions is still limited. Here, we demonstrate the role of DGKgamma in regulating Rac1-governed cell morphology. We found that the expression of kinase-dead DGKgamma, which acts as a dominant-negative mutant, and inhibition of endogenous DGKgamma activity with R59949 induced lamellipodium and membrane ruffle formation in NIH3T3 fibroblasts in the absence of growth factor stimulation. Reciprocally, lamellipodium formation induced by platelet-derived growth factor was significantly inhibited upon expression of constitutively active DGKgamma. Moreover, the constitutively active DGKgamma mutant suppressed integrin-mediated cell spreading. These effects are isoform-specific because, in the same experiments, none of the corresponding mutants of DGKalpha and DGKbeta, closely related isoforms, affected cell morphology. These results suggest that DGKgamma specifically participates in the Rac1-mediated signaling pathway leading to cytoskeletal reorganization. In support of this, DGKgamma co-localized with dominant-active Rac1 especially in lamellipodia. Moreover, we found that endogenous DGKgamma was physically associated with cellular Rac1. Dominant-negative Rac1 expression blocked the lamellipodium formation induced by kinase-dead DGKgamma, indicating that DGKgamma acts upstream of Rac1. This model is supported by studies demonstrating that kinase-dead DGKgamma selectively activated Rac1, but not Cdc42. Taken together, these results strongly suggest that DGKgamma functions through its catalytic action as an upstream suppressor of Rac1 and, consequently, lamellipodium/ruffle formation.
  • Osamu Honmou, Kiyohiro Houkin
    Neurological Surgery 32 6 571 - 577 2004年06月 [査読有り][通常論文]
  • Satoshi Kuroda, Satoshi Ushikoshi, Yoshimasa Niiya, Toshiya Osanai, Tatsuya Ishikawa, Shugo Takikawa, Kiyohiro Houkin, Yoshinobu Iwasaki
    No shinkei geka. Neurological surgery 32 6 645 - 50 2004年06月 [査読有り][通常論文]
     
    The frequency of surgical treatment for intracranial aneurysms that have been incompletely treated by endovascular coiling will increase in time. The authors describe their experience in the following 3 patients. There was one non-ruptured aneurysm and two ruptured ones. The intervals between coiling and surgery were 1, 5, and 10 months. Surgery was indicated because of partial treatment and/or growth of residual neck. Observation during surgery revealed that coils were exposed to the subarachnoid space at the tip of the aneurysm in the cases of ruptured aneurysms. When the residual neck was large enough for the size of the clip blades, there was no need to remove the coils. The coils in the neck were able to be removed when the interval between coiling and surgery was very short, but could not be removed when the interval was prolonged. Intraoperative angiography and reconstructive bypass surgery may be essential in these complex cases. As pointed out previously, the operative approach should be determined by the need for coil removal and the duration since coiling.
  • S Kuroda, T Shiga, T Ishikawa, K Houkin, T Narita, C Katoh, N Tamaki, Y Iwasaki
    JOURNAL OF NUCLEAR MEDICINE 45 6 943 - 949 2004年06月 [査読有り][通常論文]
     
    Recent studies have clarified that hemodynamically compromised patients are at high risk for subsequent stroke. The acetazolamide test is widely used to detect the patients with hemodynamic compromise due to occlusive carotid artery disease. Previous studies have suggested that patients with impaired reactivity to acetazolamide had an increased oxygen extraction fraction (OEF) on PET. However, the underlying pathophysiology has not been defined in patients with reduced blood flow and preserved reactivity to acetazolamide due to carotid occlusive diseases regardless of a normal appearance on MRI. This study aimed to clarify hemodynamic and metabolic parameters in such patients, using 150 gas and C-11-flumazenil (FMZ) PET. Methods: Our study included 15 patients who had reduced cerebral blood flow (CBF) and preserved cerebrovascular reactivity (CVR) to acetazolamide in the ipsilateral middle cerebral artery territory due to occlusive carotid diseases on N-isopropyl-p-I-123-iodoamphetamine (I-123-IMp) SPECT. We determined the CBF, cerebral metabolic rate for oxygen (CMRo(2)), cerebral blood volume (CBV), and OEF using 150 gas PET. The binding potential for C-11-FMZ was also measured in 5 patients. All patients were medically treated and followed-up during a mean period of 2.7 y. Results: 150 gas PET scans revealed that the ipsilateral CBF and CMRo(2) were reduced to 80% +/- 11% (P < 0.0001) and 78% +/- 8% (P < 0.0001) of the contralateral side, respectively. However, there was no significant side-to-side difference in the CBV and OEF The ipsilateral binding potential for C-11-FMZ was also significantly reduced to 82% +/- 2% of the contralateral side (P < 0.05), being very similar to the asymmetry of the CBF and CMRo(2). No patients suffered further ischemic stroke in the ipsilateral hemisphere during the follow-up period. Conclusion: Our results strongly suggest that a reduced CBF and a normal CVR characterize oxygen hypometabolism probably due to ischemia-related neuronal loss-namely, incomplete infarction. Such an ischemic lesion is not hemodynamically compromised and is at very low risk for a subsequent ischemic stroke even if the patient is medically treated.
  • K Kamada, Y Sawamura, F Takeuchi, K Houkin, H Kawaguchi, Y Iwasaki, S Kuriki
    JOURNAL OF NEUROSURGERY 100 6 1101 - 1106 2004年06月 [査読有り][通常論文]
     
    Letter-perception centers are not held in as high regard as motor- and language-related cortices during planning of neurosurgical procedures, and there have been no reports suggesting cortical reorganization of reading ability. The authors describe a patient with a left mesial temporal glioma in whom two letter-perception centers (the anterior portion of the left superior temporal gyrus and the left fusiform gyrus) were successfully localized before surgery by performing magnetoencephalography (MEG) during reading tasks. Control MEG examinations of 15 healthy volunteers were also performed to assist in a careful interpretation of patient results. Although a radical resection of the mesial temporal glioma, which involved the left fusiform gyrus, caused severe dyslexia, the patient's impaired reading skills improved gradually during a 1-year postoperative period. In the meantime, the spared left superior temporal gyrus displayed an overshot recovery of MEG responses. During the postoperative period there was no obvious recovery in MEG signals and no compensatory activity in the contralateral fusiform gyrus. This case demonstrates that lexicosemantic centers involved in the reading process can be noninvasively localized using MEG and that the results obtained are highly reliable for surgical planning. The results of the repeated MEG reflected sequentially the patient's recovery from dyslexia. This is the first report in which MEG studies have been shown to predict preoperatively the risk of dyslexia and demonstrate its serial physiological recovery.
  • ZY Tong, T Yamaki, K Harada, K Houkin
    MAGNETIC RESONANCE IMAGING 22 5 735 - 742 2004年06月 [査読有り][通常論文]
     
    Metabolite concentrations in normal adult brains and in gliomas were quantitatively analyzed by in vivo proton magnetic resonance spectroscopy (MRS) using the fully relaxed water signal as an internal standard. Between January 1998 and October 2001, 28 healthy volunteers and 18 patients with gliomas were examined by in vivo proton MRS. Single-voxel spectra were acquired using the point-resolved spectroscopic (PRESS) pulse sequence with a 1.5 T scanner (TR/TE/Ave = 3000 ms/30 ms/64). The calculated concentrations of N-acetyl-aspartate (NAA), creatine (Cre), choline (Cho), and water(H2O) in the normal hemispheric white matter were 23.59 +/- 2.62 mM (mean +/- SD), 13.06 +/- 1.8 mM, 4.28 +/- 0.8 mM, and,47280.96 +/- 5414.85 mM, respectively. The metabolite concentrations were not necessarily uniform in different parts of the brain. The concentrations of NAA and Cre decreased in all gliomas (p < 0.001). The NAA/Cho and NAA/H2O ratios can distinguish the normal brain from gliomas and low-grade from high-grade astrocytoma (p < 0.001). The concentration of taurine (Tau) in medulloblastomas was 29.64 +/- 5.76 mM. This is the first quantitative analysis of Tau in medulloblastoma in vivo and confirms earlier in vitro findings. (C) 2004 Elsevier Inc. All rights reserved.
  • S Kuroda, K Houkin, T Ishikawa, N Nakayama, J Ikeda, N Ishii, H Kamiyama, Y Iwasaki
    CHILDS NERVOUS SYSTEM 20 5 302 - 308 2004年05月 [査読有り][通常論文]
     
    Objects. The aim of this study was to clarify predictors for poor intellectual outcome in pediatric moyamoya disease. Methods. Fifty-two pediatric patients were included. Clinical diagnosis was transient ischemic attacks (TIA) in 35 and completed stroke in 17. Ten patients underwent indirect synangiosis through "small craniotomy," whereas the other 42 underwent superficial temporal artery (STA)-middle cerebral artery (MCA) anastomosis and indirect synangiosis through "large craniotomy." Full-scale IQ (FSIQ) was measured using the Wechsler intelligence scale for children (WISC) after surgery. Multivariate logistic regression models were applied to test the effect of clinical factors on intellectual outcome. Results and conclusion. Eight patients revealed mentally impaired status (FSIQ<70). Multivariate analysis revealed that completed stroke and "small craniotomy" surgery were significantly associated with poor intellectual outcome. Odds ratios of each factor were 33.4 (95% CI, 2.4-474) and 19.6 (95% CI, 1.8-215) respectively. Early diagnosis and the revascularization procedure over as wide an area as possible may be essential to improve their intellectual outcome.
  • S Iihoshi, O Honmou, K Houkin, K Hashi, JD Kocsis
    BRAIN RESEARCH 1007 1-2 1 - 9 2004年05月 [査読有り][通常論文]
     
    The primary objective of this study was to test the hypothesis that intravenous administration of autologous bone marrow cells could improve functional recovery after middle cerebral artery occlusion (MCAO) for 45 min in the rat and to determine specific time windows for efficacy. Mononuclear cells from autologous bone marrow were transfected with the LacZ reporter gene, and injected intravenously into rats at 3-72 h after induction of MCAO. Histological analysis of the ischemic lesion at 14 days after transplantation revealed reduced ischemic lesion volume. Lesion volume was 250 +/- 45 mm(3) (n = 6) after MCAO without cell transplantation. Lesions were minimally detected by absence of 2,3,5-triphenyltetrazolium chloride (TTC) staining when bone marrow cells were infused 3 h after lesion induction. Lesions were clearly detected beginning with the 6-h postlesion group and became progressively larger at 12, 24 and 72 h (80 +/- 25, 140 +/- 18, and 180 +/- 22 mm(3), respectively; n=6 for each group). Transplanted LacZ(+) bone marrow cells accumulated extensively in and around the ischemic lesions, and immunohistochemistry suggests some neuronal and glial lineage differentiation. Behavioral testing (Morris water maze and Treadmill stress test) indicated greater functional recovery in the treated group. These findings suggest that early intervention with intravenous administration of autologous mononuclear cells from bone marrow can reduce lesion size in the MCAO model in the rat, and improve functional outcome. (C) 2004 Elsevier B.V. All rights reserved.
  • 本間 敏美, 本望 修, 原田 邦明, 備前 明子, 劉 赫, 野村 達史, 中村 公則, 濱田 洋文, 寶金 清博
    脳卒中 26 1 185 - 185 (一社)日本脳卒中学会 2004年03月
  • Kiyohiro Houkin
    International Congress Series 1259 C 313 - 320 2004年02月01日 [査読有り][通常論文]
     
    Cerebral revascularization surgery is an important surgical option for unclippable cerebral aneurysm and prevention of the cerebral ischemia. During the past 10 years, we have performed cerebral revascularization for a variety of diseases on 257 cases. Among these cases, there were several cases of surgical complications. In adult moyamoya disease, rebleeding after surgery was seen approximately 15% in 10 years experience. High flow bypass from the external carotid artery to the middle cerebral artery was mainly performed to the unclippable carotid artery aneurysm for 56 cases. Among them, acute occlusion of the bypass was seen in three cases. Cerebral infarction occurred in one case. High flow bypass through the aorta to the occluded common carotid artery was performed in three cases of aortitis syndrome. Among them, hyperperfusion syndrome was observed in one case. In this paper, representative cases of surgical pitfalls of cerebral revascularization are demonstrated. The technical points for the prevention of these complications are discussed. © 2003, Elsevier B.V.
  • R Nanba, S Kuroda, T Ishikawa, Y Iwasaki, M Tada, H Kiyohiro
    NEUROLOGICAL SURGERY 32 1 7 - 16 2004年01月 [査読有り][通常論文]
  • F Qiao, S Kuroda, K Kamada, K Houkin, Y Iwasaki
    CHILDS NERVOUS SYSTEM 19 10-11 760 - 764 2003年11月 [査読有り][通常論文]
     
    Objects. The "re-build up" phenomenon is a pathognomonic finding induced after hyperventilation in pediatric moyamoya disease, and is closely related to ischemic attacks. However, its precise mechanism remains unknown. Methods. In this study, MEG, MRI, and SPECT were used to clarify its mechanism in 4 children with moyamoya disease. Cerebral blood flow before and after acetazolamide administration was measured. Spontaneous magnetic brain activity was measured using a whole-head MEG system. The quantified dipole distribution was shown on 3D-MRI. Results and conclusion. The original current dipoles of the "re-build up" slow waves were predominantly located in the deep cortical sulci in the area with impaired reactivity to acetazolamide. The results suggested that the re-build up waves derive from the deep cortical sulci due to the reduction in the cerebral perfusion reserve.
  • T Mikami, A Takahashi, K Houkin
    NEUROLOGIA MEDICO-CHIRURGICA 43 11 528 - 532 2003年11月 [査読有り][通常論文]
     
    B-flow ultrasonography can directly visualize blood streams by reflecting the intravascular red blood cells, and so can detect turbulent flow in vessels. B-flow sonography was used to evaluate How abnormalities in patients with cervical carotid artery stenosis, and to investigate the mechanism of large embolus formation that may cause distal arterial occlusion. Twenty-two patients with greater than or equal to60% stenosis of the cervical carotid artery excluding cardiovascular embolism were examined by B-flow and color Doppler sonography. Two patients had distal embolism as revealed by digital subtraction angiography, 16 patients had lacunar infarction, and four patients had transient ischemic attacks. B-flow sonography demonstrated reverse flow at the poststenotic site in patients with greater than or equal to70% stenosis. Increased echogenicity at the site of the stenosis was seen in patients with at least 80% stenosis. Prestenotic reverse flow was seen in nine patients with greater than or equal to85% stenosis. The two patients with distal embolism had decreased echogenicity and flow velocity, and diastolic flow was absent. B-flow sonography could detect various turbulent flow patterns and changes in echogenicity associated with cervical carotid artery stenosis. Prestenotic reverse flow and decreased flow velocity may be potential causes of distal embolism. B-How sonography is a useful method for the evaluation of flow abnormality in patients with carotid artery stenosis.
  • Tomohiro Murakami, Fumio Sakane, Shin-Ichi Imai, Kiyohiro Houkin, Hideo Kanoh
    Journal of Biological Chemistry 278 36 34364 - 34372 2003年09月05日 [査読有り][通常論文]
     
    Diacylglycerol kinase (DGK) participates in regulating the intracellular concentrations of two bioactive lipids, diacylglycerol and phosphatidic acid. DGKη(η1, 128 kDa) is a type II isozyme containing a pleckstrin homology domain at the amino terminus. Here we identified another DGKη isoform (η2, 135 kDa) that shared the same sequence with DGKη1 except for a sterile α motif (SAM) domain added at the carboxyl terminus. The DGKη1 mRNA was ubiquitously distributed in various tissues, whereas the DGKη2 mRNA was detected only in testis, kidney, and colon. The expression of DGKη2 was suppressed by glucocorticoid in contrast to the marked induction of DGKη1. DGKη2 was shown to form through its SAM domain homo-oligomers as well as hetero-oligomers with other SAM-containing DGKs (δ1 and δ2). Interestingly, DGKη1 and DGKη2 were rapidly translocated from the cytoplasm to endosomes in response to stress stimuli. In this case, DGKη1 was rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas DGKη2 exhibited sustained endosomal association. The experiments using DGKη mutants suggested that the oligomerization of DGKη2 mediated by its SAM domain was largely responsible for its sustained endosomal localization. Similarly, the oligomerization of DGKη2 was suggested to result in negative regulation of its catalytic activity. Taken together, alternative splicing of the human DGKη gene generates at least two isoforms with distinct biochemical and cell biological properties responding to different cellular metabolic requirements.
  • H Momota, S Ichimiya, N Kondo, T Kikuchi, T Torigoe, T Yamaki, K Houkin, N Sato
    INTERNATIONAL JOURNAL OF ONCOLOGY 23 2 311 - 315 2003年08月 [査読有り][通常論文]
     
    Glioblastoma is a life-threatening tumor in the human brain despite the fact that radio-chemotherapy inducing DNA damage has been improved in the last decade. Various studies focusing on the enhancement of the susceptibility of glioblastoma cells to DNA damage have been reported, which are aimed at more efficient treatment for the tumor. In this study, we show that radioresistant T98G glioblastoma cells can develop sensitivity to DNA damage induced by irradiation and etoposide as a result of the introduction of a DNA repair-associated historic, H2AX. Interestingly, when H2AX-transformed T98G cells were irradiated, Brca1 and Nbs1 were readily recruited in DNA double-strand break (DSB) foci and showed the G2/M-phase arrest of the cell cycle. Moreover, up-regulation of Brcal was observed in H2AX-T98G cells after exposure to irradiation. Together with the evidence that H2AX transfection does not affect growth activities of non-tumor cells under genotoxic stimuli, this suggests that H2AX gene transfer would provide a new modality for radio-chemotherapy for glioblastomas, probably through overcoming the instability of the genome, and that Brca1 and Nbs1 might be crucial in this methodology.
  • M Kobune, Y Kawano, Y Ito, H Chiba, K Nakamura, H Tsuda, K Sasaki, H Dehari, H Uchida, O Honmou, S Takahashi, A Bizen, R Takimoto, T Matsunaga, J Kato, K Kato, K Houkin, Y Niitsu, H Hamada
    EXPERIMENTAL HEMATOLOGY 31 8 715 - 722 2003年08月 [査読有り][通常論文]
     
    Objective. To compare the hematopoietic support provided by telomerized human mesenchymal stem cells (MSCs) and telomerized MSC-derived stromal cells. Methods. We transfected the human telomerase catalytic subunit (hTERT) gene into primary MSCs to establish hTERT-transduced MSCs (hTERT-MSCs). Stromal induction of hTERT-MSCs was performed by replacing the culture medium with Dexter-type culture medium. Hematopoietic support was examined by coculture with cord blood CD34(+) cells. Results. The hTERT-MSCs were morphologically identical with the primary MSCs and expressed surface antigens including CD105, CD73, and CD166. hTERT-MSCs showed a similar doubling time as primary MSCs and continued to proliferate to over 80 population doublings (PD), although the primary MSCs underwent crisis in vitro at 16 PD. The osteogenic, chondrogenic, adipogenic, neurogenic, and stromal differentiation potential of hTERT-MSCs were maintained up to at least 40 PD. The degree of expansion of CD34+ cells and total number of colony-forming units in culture (CFU-C) upon 12-day coculture with the hTERT-MSC-derived stromal cells were nearly the same as those upon 12-day coculture with hTERT-MSCs (CD34, 33.0-fold +/- 2.8-fold vs 36.1-fold +/- 1.7-fold of the initial cell number; CFUs, 344.4-fold +/- 62.5-fold vs 239.3-fold +/- 87.0-fold; CFU-mix, 368.4-fold +/- 113.7-fold vs 341.3-fold +/- 234.3-fold). However, on day 18 of coculture, the number of cobblestone areas (CA) observed beneath the stromal cells was 15 times higher than that beneath hTERT-MSCs (CA, 146.9 +/- 54.6 vs 9.4 +/- 8.1, p < 0.01). Conclusion. Stromal induction of hTERT-MSCs exclusively enhanced the support of CA formation provided by hTERT-MSCs. Our human hTERT-MSCs will be useful for elucidating the mechanism of the formation of CAs. (C) 2003 International Society for Experimental Hematology. Published by Elsevier Inc.
  • 間葉系幹細胞(MSC)を用いた浸潤性グリオーマ遺伝子治療の開発
    中村 公則, 伊藤 克礼, 河野 豊, 黒住 和彦, 佐々木 勝則, 備前 明子, 本望 修, 宝金 清博, 濱田 洋文
    日本癌学会総会記事 62回 325 - 325 日本癌学会 2003年08月 [査読無し][通常論文]
  • S Kin, M Wanibuschi, Y Minamida, T Yamaki, S Tanabe, K Houkin
    NEUROLOGICAL SURGERY 31 6 677 - 682 2003年06月 [査読有り][通常論文]
     
    We report a case of a 66-year-old male with diffuse infiltration of neoplasm from the paranasal sinuses to the orbit and left cerebral hemisphere, associated with prominent edema. Initial complaints were headache and swelling of the forehead, which were followed by progressive symptoms, Such as epistaxis, dacryops, and severe pains. Neuroimaging showed marked invasion of a neoplasm from the left paranasal sinuses into the intracranial space and to the frontal skull.Radical removal of the neoplasm and left orbital content with reconstruction of the anterior skull base using musclocutaneous flap was carried out by a multidisciplinary team. The pathological diagnosis was "poorly differentiated carcinoma from the paranasal sinus". Postoperatively the patient received radiation and chemotherapy. He is still alive with mild right hemiparesis and mild dysphasia, more than 2 years after surgery.
  • K Kamada, K Houkin, F Takeuchi, N Ishii, J Ikeda, Y Sawamura, S Kuriki, H Kawaguchi, Y Iwasaki
    SURGICAL NEUROLOGY 59 5 353 - 362 2003年05月 [査読有り][通常論文]
     
    BACKGROUND In this study, we visualized the eloquent motor system including the somatosensory-motor cortex and corticospinal tract on a neuronavigation system, integrating magnetoencephalography (MEG), functional magnetic resonance imaging (fMRI), and anisotropic diffusion-weighted MRI (ADWI). METHODS Four patients with brain lesions adjacent to the eloquent motor system were studied. Motor-evoked responses (MER) by finger-tapping paradigm were acquired with a 1.5-Tesla MR scanner, and somatosensory-evoked magnetic fields (SEF) by median nerve stimulation were measured with a 204-channel MEG system. In the same fMRI examination, ADWI and anatomic three-dimensional T1-weighted imaging (3-D MRI) were obtained. Activated areas of MER, estimated SEF dipoles, and the corticospinal tract on ADWI were coregistered to 3-D MRI, and the combined MR data were transferred to a neuronavigation system (functional neuronavigation). Intraoperative recording of cortical somatosensory-evoked potentials was performed for confirmation of the central sulcus. RESULTS Combination of fMRI and MEG enabled firm identification of the central sulcus. Functional neuronavigation facilitated extensive tumor resection, having the advantage of sparing the motor cortex and corticospinal tract in all cases. CONCLUSIONS The proposed functional neuronavigation allows neuro-surgeons to perform effective and maximal resection of brain lesions, identifying and sparing eloquent cortical components and their subcortical connections. Potential clinical application of this technique is discussed. (C) 2003 Elsevier Inc. All rights reserved.
  • 穂刈 正昭, 黒田 敏, 古川 浩司, 宝金 清博, 岩崎 喜信
    Neurological Surgery 31 3 281 - 286 (株)医学書院 2003年03月 [査読無し][通常論文]
     
    右大動脈弓及び左鎖骨下動脈閉鎖により,成人期になってから鎖骨下動脈盗血症候群(SSS)を呈したPeutz-Jeghers症候群の症例(29歳男性)を経験し,この症例に対して左総頸動脈-頸横動脈吻合術による血行再建術を実施した.その結果,脳血管撮影上のSSSの消失,脳循環動態の改善,めまい発作の長期間消失を認め,本手術の有効性が示唆された
  • S Kuroda, T Kobayashi, N Ishii, J Ikeda, Y Shinohe, K Houkin, Y Iwasaki, K Nagashima
    NEUROPATHOLOGY 23 1 1 - 8 2003年03月 [査読有り][通常論文]
     
    Chlamydia pneumoniae (C. pneumoniae) infection has been recently accepted as an important cause of atherosclerosis. However, the precise mechanisms remain unclear. The present study was aimed to clarify the distribution link among C. pneumoniae , chlamydial HSP 60, and activated macrophages. Atheromatous carotid plaques were obtained from 40 consecutive carotid endarterectomies (CEA). The specimens were prepared for HE and elastica-van Gieson staining. Parallel sections were stained immunocytochemically with monoclonal antibodies for a C. pneumoniae -specific antigen, chlamydial HSP 60, activated macrophages, and smooth muscle cells. Immunoreactivity for the C. pneumoniae-specific antigen was observed within the endothelial cells, activated macrophages, and smooth muscle cells in 36 of 40 specimens (90%). Chlamydial HSP 60 was found in all specimens positive for the C. pneumoniae-specific antigen, and mainly co-localized with the C. pneumoniae -specific antigen within the activated macrophages. The present results suggest that C. pneumoniae is a key microbial organ that causes atheroma developments in the carotid artery. Chlamydia pneumoniae -infected macrophages may come into the arterial intima and mediate inflammatory and autoimmune processes through the production of chlamydial HSP 60, leading to atherosclerosis.
  • T Kobayashi, S Kuroda, M Tada, K Houkin, Y Iwasaki, H Abe
    BRAIN RESEARCH 960 1-2 62 - 70 2003年01月 [査読有り][通常論文]
     
    The objective of the present study, was to determine the biochemical characteristic of Ca2+-induced mitochondrial swelling (mitochondrial permeability transition: mPT) and cytochrome c release in the brain. and to clarify its role in neuronal injury following transient forebrain ischemia. Mitochondria were isolated from rat brain and liver. Changes in mitochondrial volume were measured via light absorbance at 540 nm. Using Western blot analysis, we examined the in vitro release of mitoehondrial cytochrome c under these conditions. Transient forebrain ischemia was induced by 5 min occlusion of the common carotid arteries in the gerbil, Cyclosparin A (CsA), a specific mPT blocker, and/or trifluoperazine. a blocker of phospholipase were given before and 24 h after ischemia. The number of surviving neurons in the hippocampal CA1 sector was counted 7 days after ischemia. Calcium induced a moderate decrease of light absorbance in brain mitochondria. which was inhibited by CsA. However, Calcium induced a much larger decrease of light absorbance in liver mitochondria. Calcium induced a moderate release of cytochrome c from brain mitochondria. which was not inhibited by CsA. However. calcium induced the release of a larger amount of cytochrome c from liver mitochondria. Selective neuronal injury due to transient forebrain ischemia was significantly ameliorated by treatment with high-dose CsA. The biochemical properties of Ca2+-induced mitochondrial swelling in the brain are different from those in the liver. Cytochrome c is released from brain mitochondria through in mPT-independent mechanism. CsA potentially ameliorates delayed neuronal injury in file hippocampus due to transient forebrain ischemia. (C) 2002 Elsevier Science B.V. All rights reserved.
  • A Bizen, O Honmou, M Inoue, S Iihoshi, K Houkin, K Hashi
    MOLECULAR MECHANISMS AND EPOCHAL THERAPEUTICS OF ISCHEMIC STROKE AND DEMENTIA 1252 471 - 475 2003年 [査読有り][通常論文]
     
    Transplantation of a cell fraction acutely isolated from bone marrow into the demyelinated spinal cords causes the extensive remyelination in the adult rats. The crude cell fraction that remyelinated the demyelinated axons in the spinal cords may contain several types of precursors and stem cells such as hematopoietic stem cells, nonhematopoietic stem cells, and other unknown precursor cells. In the present study, we examined whether the mesenchymal stem cells (MSCs) in the bone marrow have the potential to differentiate into a neural lineage and repair the demyelinating spinal cord following transplantation. A focal demyelinated lesion was created in the dorsal columns of the rat spinal cord using X-irradiation and ethidium bromide injection (EB-X). A suspension of human mesenchymal stem cells collected from the human bone marrow was injected into the middle of the EB-X-induced lesion 3 days after the EB injection. Transplant-receiving rats were immunosuppressed with cyclosporin A. Lesions were examined histologically 3 weeks after transplantation. Light and electron microscopic examination revealed that the demyelinated axons were extensively remyelinated by transplanted mesenchymal stem cells. Thus, mesenchymal stem cells in the bone marrow may be a source for an auto-transplantation therapy of the demyelinating diseases. (C) 2003 Elsevier Science B.V. All rights reserved.
  • S Kuroda, T Ishikawa, K Houkin, Y Iwasaki
    NEUROLOGICAL SURGERY 30 12 1295 - 1300 2002年12月 [査読有り][通常論文]
     
    Purpose : The present study was aimed at clarifying the clinical significance of posterior cerebral artery (PCA) stenosis/occlusion in pediatric and adult moyamoya disease. Subjects and Methods : This study included a total of 132 patients (52 children and 80 adults) who were diagnosed as by cerebral angiography having moyamoya disease. CT or MRI was performed to examine the location of cerebral infarction in all subjects. Cerebral blood flow and vasoreactivity to acetazolamide were measured in 80 patients before surgery, using single photon emission computed tomography (SPECT). Three-dimensional MR angiography (3D-MRA) was repeated in 32 pediatric patients after surgery in order to clarify the natural course of the PCA stenosis/occlusion. Results : Of 264 sides in 132 patients, PCA stenosis/occlusion was observed in 50 sides of 40 patients (30.3%). Its incidence was significantly higher in ischemic-type patients than in hemorrhagic-type and asymptomatic patients, and was higher in patients in the advanced stage of the disease. The hemisphere ipsilateral to PCA stenosis/occlusion had higher incidence of ischemic symptoms, cerebral infarction, and impaired cerebral hemodynamics. TIA (hemianopsia) or cerebral infarction in the occipital lobe was noted in 4 (10%) of 40 patients during follow-up periods after bypass surgery for anterior circulation. Of 32 pediatric patients, none showed progression of PCA stenosis on 3D-MRA during follow-up periods. Conclusions : The present study showed that the involvement of PCA could increase the risk of TIA and/or cerebral infarction in both anterior and posterior circulation areas, suggesting that the PCA plays an important collateral role in moyamoya disease.
  • J Kitamura, S Kuroda, S Ushikoshi, K Furukawa, T Asano, H Shichinohe, K Houkin, Y Iwasaki, H Saitoh, K Mitsumori
    NEUROLOGICAL SURGERY 30 10 1097 - 1102 2002年10月 [査読有り][通常論文]
     
    We report three cases of radiation-induced carotid arterial stenosis that underwent successful angioplasty with stenting. The patients had received radiation therapy for tongue or laryngeal cancers and developed minor completed strokes 6 to 14 years after irradiation. All patients had multiple and bilateral stenosis, measuring more than 50%, of the carotid arteries. The stenosis was located in the internal, external, and common carotid arteries. We performed percutaneous transluminal angioplasty with stenting. All interventions were successful and carotid stenosis decreased to less than 28%. No permanent complications occurred. During follow-up periods of Lip to 26 months, all of these cases were free from ischemic symptoms. Neither carotid angiography nor ultrasound sonography showed evidence of restenosis. The present results suggest the usefulness of angioplasty with stenting for radiation-induced carotid arterial steriosis.
  • K Kamada, K Houkin, Y Iwasaki, F Takeuchi, S Kuriki, K Mitsumori, Y Sawamura
    JOURNAL OF NEUROSURGERY 97 3 558 - 567 2002年09月 [査読有り][通常論文]
     
    Object. To identify the primary motor area (PMA) quickly and correctly, the authors used magnetic resonance (MR) axonography, including anisotropic diffusion-weighted (DW) MR imaging and three-dimensional anisotropic contrast (3DAC) imaging, which was performed to visualize the corticospinal tract mainly originating from the PMA. Methods. All studies were obtained in 10 normal volunteers and in 17 patients with brain tumors affecting the central motor system. Data sets of anisotropic DW imaging and anatomical and functional (f)MR imaging were acquired while the participants executed simple hand movements. Offline processing of 3DAC MR axonography images was subsequently done to extract only the anisotropic components of the tract fibers. Somatosensory evoked fields (SSEFs) and intraoperative cortical somatosensory evoked potentials (SSEPs) were recorded after electrical stimulation of the median nerve. Conclusions. In normal volunteers, anisotropic DW imaging, 3DAC imaging, fMR imaging, and magnetoencephalography consistently localized the PMA in both hemispheres. In contrast, fMR imaging and SSEFs failed to identify the PMA in seven and one of the 17 patients, respectively, because of cortical dysfunctions due to brain tumor. The anisotropic DW imaging data acquired within 30 seconds with no patient tasks successfully identified the PMA in 12 patients, and failed in five patients because of the lesions involving the frontal lobe. The anisotropic axonal components were distinctly visualized on 3DAC images and indicated the PMA location, which was confirmed on intraoperative SSEPs in all 17 affected hemispheres. Swift and noninvasive PMA identification by rapid scanning with MR axonography is a promising method for routine clinical use and is especially beneficial for patients who have severe cortical dysfunction in the PMA.
  • S Ushikoshi, K Houkin, S Kuroda, T Asano, Y Iwasaki, K Miyasaka, H Abe
    SURGICAL NEUROLOGY 57 4 253 - 261 2002年04月 [査読有り][通常論文]
     
    BACKGROUND When considering the treatment strategies for dural arteriovenous fistulas (DAVFs), it is important to clarify the exact location of the fistula and venous drainage route from both DAVFs and normal brain tissue. DAVFs with leptomeningeal retrograde venous drainage carry a high risk of neurological deficits and require aggressive treatment. When AVFs involve the dural sinus, transvenous embolization via the transfemoral approach is usually the first choice of treatment. For DAVFs draining directly into the cortical veins without dural sinus involvement, transarterial embolization may be a curative treatment. However, when embolization is technically difficult or results in incomplete occlusion, surgical treatment is required. The purpose of the present study was to review our experience with surgical treatment of DAVFs. METHODS The present series included 17 patients; 9 had DAVFs involving the dural sinus with leptomeningeal retrograde venous drainage and 8 had DAVFs draining directly into the cortical veins. For DAVFs involving the sinus, embolization of the diseased sinus by direct surgical exposure was performed in 8 patients, and surgical excision in one. For DAVFs draining directly into the cortical veins, interruption of the draining veins close to DAVFs was undertaken in 7 and surgical excision in 1. RESULTS Complete obliteration of DAVFs was demonstrated in 16 patients. At final follow-up, 15 patients were asymptomatic and the other 2 showed clinical improvement. CONCLUSIONS For DAVFs involving the dural sinus, direct operative sinus packing is indicated. For DAVFs directly draining into the cortical veins, surgical interruption of the draining veins is indicated. (C) 2002 by Elsevier Science Inc.
  • S Yano, K Houkin, S Abe, H Saitoh, S Kuroda, T Asano, S Ushikoshi, Y Iwasaki
    NEUROLOGICAL SURGERY 29 11 1081 - 1090 2001年11月 [査読有り][通常論文]
     
    Patients with intracranial vertebral artery (VA) or basilar artery (BA) stenosis have been mainly treated with medication. However, it has been reported that about 10% of patients experience restroke in the VBA territory, with a high rate of morbidity and mortality. Percutaneous transluminal angioplasty (PTA) is regarded as one of the treatments although it carries the risk of restenosis, recoil or dissection. Stent placement is, therefore, considered to be a further option for the treatment of intracranial artery stenosis since it can prevent the restenosis. We report three medical refractory cases of intracranial VA (2 cases) or BA stenosis (1 case) treated with stenting, none of which had complications and their symptoms disappeared or diminished. Angioplasty with the use of a stent device is regarded as a useful treatment for intracranial artery stenosis, but its risk and long-term result need to be investigated further.
  • S Kuroda, K Houkin, H Kamiyama, H Abe
    NEUROSURGERY 49 2 463 - 467 2001年08月 [査読有り][通常論文]
     
    OBJECTIVE AND IMPORTANCE: The beneficial effects of surgical revascularization on rebleeding in moyamoya disease remain unclear. This report is intended to clarify the effects of surgical revascularization on peripheral artery aneurysms, which represent one of the causes of intracranial bleeding in moyamoya disease. CLINICAL PRESENTATION: Findings for three female patients who experienced intracranial bleeding are presented. Cerebral angiography revealed that intracranial bleeding resulted from the rupture of peripheral artery aneurysms arising from dilated collateral vessels such as the lenticulostriate artery. INTERVENTION: The patients successfully underwent superficial temporal artery-middle cerebral artery anastomosis combined with encephaloduromyoarteriosynangiosis. Angiography demonstrated obliteration of the peripheral artery aneurysms, together with the disappearance or decrease in caliber of the parent collateral arteries, after surgery. None of the patients experienced rebleeding during the follow-up period (up to 52 mo). CONCLUSION: The results strongly suggest that surgical revascularization potentially improves cerebral circulation and decreases hemodynamic stress on collateral vessels, obliterating peripheral artery aneurysms.
  • 黒田 敏, 宝金 清博, 中山 若樹, 成田 拓人, 上山 博康, 岩崎 喜信
    小児の脳神経 26 4 325 - 325 (一社)日本小児神経外科学会 2001年08月 [査読無し][通常論文]
  • 中山 若樹, 宝金 清博, 黒田 敏, 岩崎 喜信
    小児の脳神経 26 4 271 - 271 (一社)日本小児神経外科学会 2001年08月 [査読無し][通常論文]
  • 中山 若樹, 宝金 清博, 斎藤 久壽
    脳循環代謝 13 2 106 - 107 (一社)日本脳循環代謝学会 2001年07月 [査読無し][通常論文]
  • 中山 若樹, 黒田 敏, 宝金 清博, 齋藤 伸治, 岩崎 喜信, 阿部 弘
    脳卒中 23 1 90 - 90 (一社)日本脳卒中学会 2001年03月 [査読無し][通常論文]
  • S Kuroda, K Houkin, M Nunomura, H Abe
    NEUROLOGIA MEDICO-CHIRURGICA 40 6 315 - 320 2000年06月 [査読有り][通常論文]
     
    A 60-year-old female and a 40-year-old male underwent surgical revascularization for moyamoya disease and suffered small infarction in the ipsilateral frontal lobe 3 or 4 days postoperatively. Neuroimaging suggested that the bypass flow had caused rapid progression of occlusive changes in the carotid forks, a diminishing of moyamoya vessels, and flow reduction in the anterior cerebral artery ipsilateral to surgery, leading to critical ischemia in the frontal lobe. Surgical revascularization improves the outcome of patients with moyamoya disease, but postoperative management such as hydration is important to avoid ischemic complications due to frontal lobe infarction.
  • K Houkin, S Kuroda, T Ishikawa, H Abe
    ACTA NEUROCHIRURGICA 142 3 269 - 276 2000年 [査読有り][通常論文]
     
    The effects of direct and indirect revascularization for moyamoya disease were analyzed for each donor artery to determine which surgical procedure is most useful for the induction of neovascularization. In the past 12 years, 85 patients with moyamoya disease were surgically treated by combined surgery consisting of indirect revascularization via encephalo-duro-arterio-myo-synangiosis (EDAMS) and direct revascularization via the superficial temporal artery and the middle cerebral artery (STA-MCA) bypass. Among those patients, the post-operative changes in digital subtraction angiography were examined in 56 sides, including 34 sides in paediatric cases and 22 sides in adult cases. The neovascularization after indirect revascularization using the 1) superficial temporal artery (skin), 2) middle meningeal artery (dura mater), 3) deep temporal artery (temporal muscle) was analyzed. As results, in paediatric cases, the deep temporal artery and middle meningeal artery induced good neovascularization. However, the induction of neovascularization from the superficial temporal artery was not always good in most pediatric and adult cases. On the other hand, the direct bypass was useful in 90% of adult cases. In indirect revascularization surgery for moyamoya disease, the temporal muscle (the deep temporal artery) and the dura mater (the middle meningeal artery) are useful donors to the ischemic brain. The simple encephalo-arterio-synangiosis is not always effective. The direct bypass is a useful technique for adult moyamoya disease.
  • Satoshi Ushikoshi, Yoichi Kikuchi, Kiyohiro Houkin, Kazuo Miyasaka, Hiroshi Abe
    Neurologia Medico-Chirurgica 39 7 524 - 529 1999年 [査読有り][通常論文]
     
    An 81-year-old male presented with right oculomotor nerve paresis and left hemiparesis caused by a mass effect of a large superior cerebellar artery aneurysm. Endovascular treatment was performed using Guglielmi detachable coils. The patient subsequently suffered aggravation of the mass effect 3 weeks after the embolization. Bilateral vertebral artery occlusion was performed, which decreased the cerebral edema surrounding the aneurysm, but his neurological symptoms did not improve. Parent artery occlusion is recommended as the first choice of treatment for an unclippable large or giant aneurysm causing a mass effect on the brainstem. © 1999, The Japan Neurosurgical Society. All rights reserved.
  • K Houkin, S Kuroda, A Takahashi, S Takikawa, T Ishikawa, T Yoshimoto, K Itamato
    ACTA NEUROCHIRURGICA 141 12 1255 - 1263 1999年 [査読有り][通常論文]
     
    The causes and management of intra-operative premature rupture are analysed and discussed. During the past 6 years, the authors, performed 398 consecutive direct surgical interventions for ruptured cerebral aneurysms. Intraoperative premature rupture is defined as a rupture which occurs before the securing of the parent arteries or the neck of the aneurysm and is out of control, at least temporarily. The causes and management were retrospectively analysed by reviewing video tape recordings. Intra-operative premature ruptures which met the above definition occurred in 24 cases (6.0%). The causes were as follows: 1.) dural opening and arachnoid opening (8.3%), 2.) haematoma removal (12.5%), 3.) brain retraction (16.7%), 4.) aneurysm dissection (62.5%). A double suction technique was used to control bleeding and haemostasis with a small piece of cotton or a temporary clip, performed in 20 cases (83.3%). However, in cases with premature rupture immediately after the dural or arachnoid opening, the extension of the haematoma into the subarachnoid space resulted in severe brain swelling and partial resection of the brain had to be done to secure temporary clipping. The double suction technique and primary haemostasis using a small piece of cotton or temporary clip resulted in good outcome even in cases with premature rupture. However, very early premature rupture also occurred although its incidence was extremely rare. The removal of part of the brain can secure the working space but the outcome was poor.
  • K Houkin, T Ishikawa, S Kuroda, H Abe
    NEUROSURGERY 43 3 501 - 505 1998年09月 [査読有り][通常論文]
     
    OBJECTIVE: This study presents the relationship between the patency of short-vessel graft bypasses and their diameter/length. METHODS: The authors performed interposed graft bypass operations using small vessels for four patients with moyamoya disease, six patients with cerebral thrombosis, and one patient with aortitis syndrome. The donor artery was the superficial temporal artery (10 patients) or the occipital artery (1 patient), and the recipient artery was the cortical branch of the middle cerebral artery (8 patients) or the cortical branch of the anterior cerebral artery (3 patients). The interposed graft used between these donor and recipient vessels was the superficial temporal vein (seven patients), the superficial temporal artery (three patients), or the epigastric artery (one patient). RESULTS: Good patency of the graft was confirmed for 7 of these 11 patients. Regarding the relationship between the diameter/length and the patency, we found that long-term patency could not be expected when the discriminant function of y = (15.39 x diameter) - (0.35 x length) - 14.37 was below zero. CONCLUSION: Short-vessel graft bypass is a practical option for cerebral revascularization surgery when short large vessels are used.
  • Tetsuyuki Yoshimoto, Yutaka Sawamura, Kiyohiro Houkin, Hiroshi Abe
    Neurologia Medico-Chirurgica 37 12 886 - 890 1997年12月 [査読有り][通常論文]
     
    Cerebrospinal fluid (CSF) leakage through dural sutures after craniotomy increases the risk of complications such as persistent CSF fistulas, meningitis, and subcutaneous, graft bone, or epidural infections. The effectiveness of fibrin glue for preventing CSF leakage through dural closure was investigated in 183 patients who underwent frontotemporal craniotomy for unruptured aneurysm. Fibrin glue was used in 138 patients, but not in the other 45. Computed tomography was used to evaluate postoperative extradural fluid collection as a minimal volume of low-density area. Postoperative computed tomography demonstrated transient increases in extradural fluid collection in 55 patients. However, none of these patients received a second surgery for dural repair. Fluid collection occurred in 19 of 45 patients (42%) in whom fibrin glue was not used, and in 36 of 138 patients (26%) in whom fibrin glue was used. The fibrin sealant on the dural suture was a significant factor in reducing the incidence of the fluid leakage (p < 0.05, chi-square test). Fibrin glue is a useful surgical tool for the prevention of postoperative extradural fluid collection through the dural sutures.
  • Adnan Abd Rahman Zurin, Kiyohiro Houkin, Tsuyoshi Asano, Tatsuya Ishikawa, Hiroshi Abe
    Neurologia Medico-Chirurgica 37 7 542 - 545 1997年07月 [査読有り][通常論文]
     
    An 8-year-old girl presented with fibromuscular dysplasia of the intracranial vessels manifesting as ischemic stroke. Neuroimaging showed infarction of the right putamen and ipsilateral frontal white matter. Angiography revealed 'string of beads' sign involving the terminal portion of the right internal carotid artery and the horizontal segment of the ipsilateral middle cerebral artery. She was treated conservatively. Magnetic resonance angiography at 2 months post ictus showed similar findings in the middle cerebral artery but improvement of the stenosis of the internal carotid artery. Her neurological deficits had almost resolved. Fibromuscular dysplasia should be part of the differential diagnosis of ischemia in children.
  • Kyousuke Kamada, Kiyohiro Houkin, Hiroshi Abe, Yutaka Sawamura, Takeshi Kashiwaba
    Neurologia Medico-Chirurgica 37 3 250 - 256 1997年03月 [査読有り][通常論文]
     
    Proton magnetic resonance spectroscopy (3H MRS) was evaluated for distinguishing between radiation necrosis and recurrent glioma in 11 patients after high-dose radiotherapy. Six patients had a histological diagnosis of recurrent glioma. Four patients had a histological diagnosis of radiation necrosis and one had a clinical course consistent with the diagnosis of radiation necrosis. 3H MRS showed cases of radiation necrosis had two characteristic 1H MRS patterns: markedly increased lactate/creatine and phosphocreatine (Cr) ratio and decreased choline-containing compounds/Cr ratio compared to that of recurrent glioma or all the major metabolites were completely diminished. The N-acetyl aspartate signal was not helpful for differential diagnosis. 1H MRS is a potentially useful method for differentiating tumor recurrence from radiation necrosis in patients treated for malignant glioma.
  • T Ishikawa, N Nakamura, K Houkin, M Nomura
    NEUROSURGERY 40 2 403 - 405 1997年02月 [査読有り][通常論文]
     
    OBJECTIVE AND IMPORTANCE: Although the incidence is low, a very small aneurysm with a thin wall and no neck arises at the superior wall of the supraclinoid portion of the internal carotid artery and is called a ''blister-like'' aneurysm. However, the pathogenesis of such a vascular lesion remains uncertain. CLINICAL PRESENTATION: A 57-year-old man developed a fatal subarachnoid hemorrhage caused by the rupture of a blister-like aneurysm at the superior wall of the internal carotid artery. An autopsy was performed, and the lesion was pathologically examined. RESULTS: The internal elastic lamina and media had disappeared at the border between the eccentrically sclerotic and normal carotid wall. The gap in the internal elastica was covered with normal adventitia and fibrinous tissue. This portion was not composed of collagenous tissue as ordinarily seen in an aneurysmal wall. Neither infiltration of inflammatory cells nor dissection of the artery were observed. CONCLUSION: The blister-like aneurysm appeared to be a laceration of the carotid wall based on degeneration of the internal elastic lamina.
  • T Yoshimoto, K Houkin, A Takahashi, H Abe
    STROKE 27 12 2160 - 2165 1996年12月 [査読有り][通常論文]
     
    Background and Purpose We previously reported that the level of basic fibroblast growth factor (bFGF) is high in cerebrospinal fluid (CSF) taken from patients with moyamoya disease. The present study investigated the levels of other angiogenic growth factors in the CSF of moyamoya patients and the clinical significance of bFGF in moyamoya disease. Methods The levels of bFGF, interleukin-8, platelet-derived growth factor, transforming growth factor-beta, endothelial growth factor, and vascular endothelial cell growth factor in CSF, taken from 38 patients with moyamoya disease and 16 patients with atherosclerotic occlusive disease (control group), were measured by an enzyme-linked immunosorbent assay. We analyzed the correlation between the level of bFGF and the clinical factors of age, onset pattern, development of neovascularization, and cerebral circulation. Results The CSF of moyamoya patients contained a high concentration of bFGF to a significant (P<.05) extent. The bFGF level was apparently elevated in the patients in whom neovascularization from indirect revascularization, such as encephaloduroarteriosynangiosis, was well developed (P<.01). A linear correlation between the values of bFGF and cerebral vascular response to acetazolamide (r=.7; P<.05) was revealed. The other angiogenic factors were not significantly high compared with the control group. Conclusions The elevation of bFGF in moyamoya disease seems to be specific and is not related simply to cerebral ischemia. Clinically, the bFGF level is a useful indicator to predict the efficacy of indirect revascularization after surgery.
  • K Kazumata, N Tanaka, T Ishikawa, S Kuroda, K Houkin, K Mitsumori
    STROKE 27 11 2052 - 2058 1996年11月 [査読有り][通常論文]
     
    Background and Purpose The aim of this study was to com pare the effect of vasodilative stimuli for the measurement of cerebrovascular reactivity obtained by acetazolamide and hypercapnia in patients with chronic occlusive major cerebral artery disease. Methods We examined 24 patients with unilateral occlusive lesions of a major cerebral artery using the Xe-133 inhalation technique and single-photon emission CT. Regional cerebral blood Row (CBF) was measured during a resting state, during inhalation of 5% CO2, and 15 minutes after the administration of acetazolamide consecutively in the same patients. Normative values of resting CBF and acetazolamide reactivity were obtained in 21 normal subjects. Results All patients with the exception of 1 showed an increase in CBF during hypercapnia ipsilateral to the occlusive lesion. Ipsilateral acetazolamide reactivity was preserved in 13 patients. Conversely, 11 patients showed an absent response or paradoxical CBF reduction. Ipsilateral CO2 reactivity did not correlate with acetazolamide reactivity when all 24 patients were considered. However, there was a significant correlation between acetazolamide and CO2 in the 13 patients who showed preserved acetazolamide reactivity (r=.60, P<.05). No significant correlation was present in the remaining 11 patients with reduced acetazolamide reactivity. Although significant blood pressure augmentation was observed in hypercapnia, we could not find a correlation between change of blood pressure and CO2 reactivity. Conclusions Acetazolamide identified patients with reduced vasomotor reactivity who appeared to have preserved CO2 reactivity. Acetazolamide testing may be useful in the assessment of cerebral hemodynamics. However, further investigations are necessary to assess the clinical utility of these tests.
  • S Kuroda, K Houkin, Y Hoshi, M Tamura, K Kazumata, H Abe
    CHILDS NERVOUS SYSTEM 12 8 448 - 452 1996年08月 [査読有り][通常論文]
     
    Near-infrared spectroscopy was used to monitor the sequential changes in the cerebral oxygenation state during and after hyperventilation in two children with moyamoya disease. Hyperventilation induced the build-up phenomenon and a decrease in the concentration of oxy-hemoglobin ([oxy-Hb]) and total hemoglobin ([t-Hb]). The termination of hyperventilation was followed by partial recovery of [oxy-Hb] and [t-Hb], Subsequently, however, [oxy-Hb] and [t-Hb] decreased again and cytochrome oxidase was reduced. These impairments of the cerebral hemodynamics and oxygen metabolism were closely associated with the re-build-up phenomenon on EEG and with transient ischemic attacks (TIA). The present study implies that cerebral hypoxia after hyperventilation is closely related to the re-build-up phenomenon and ischemic attacks in children with moyamoya disease.
  • S Kuroda, K Houkin, H Abe, K Mitsumori
    NEURORADIOLOGY 38 3 239 - 242 1996年04月 [査読有り][通常論文]
     
    Remote cerebral hypoperfusion associated with pontine lesions is rare, We describe a patient who showed transient ipsilateral hypoperfusion in the thalamus and cerebral cortex after a pontine infarct, This resolved within 8 days after onset. Anatomical considerations strongly suggested involvement of the cerebellothalamocortical pathway in this case.
  • Kiyohiro Houkin, Hiroshi Abe, Yuji Hashiguchi, Shigemi Seri
    Neurologia Medico-Chirurgica 36 2 72 - 77 1996年02月 [査読有り][通常論文]
     
    The chronological changes of blood-brain barrier disruption, and diffusion and absorption of edema fluid were investigated in rats with cold-induced brain injury (vasogenic edema) using magnetic resonance imaging. Contrast medium was administered intravenously at 3 and 24 hours after lesioning as a tracer of edema fluid. Serial T1-weighted multiple-slice images were obtained for 180 minutes after contrast administration. Disruption of the blood-brain barrier was more prominent at 24 hours after lesioning than at 3 hours. Contrast medium leaked from the periphery of the injury and gradually diffused to the center of the lesion. Contrast medium diffused into the corpus callosum and the ventricular system (cerebrospinal fluid). Disruption of the blood-brain barrier induced by cold injury was most prominent at the periphery of the vasogenic edema. Edema fluid subsequently extended into the center of the lesion and was also absorbed by the ventricular system. Magnetic resonance imaging is a useful method to assess the efficacy of therapy for vasogenic edema.
  • S. Kawaguchi, K. Kamada, K. Itamoto, H. Ohtuki, K. Isonishi, N. Nakayama, M. Shimoyama, K. Tokuda, M. Koiwa, T. Kashiwaba, K. Houkin
    Brain and Nerve 47 1 43 - 48 1995年 [査読有り][通常論文]
     
    The authors assessed proton magnetic resonance spectroscopy (1H-MRS) in patients with unilateral refractory temporal bole epilepsy. The subjects consisted of 20 patients (those with brain tumors, trauma, malformations or definite organic lesions were excluded) and 10 normal volunteers. 1H-MRS and MRI were performed using a 1.5 tesla machine. 1H-MRS was achieved using point-resolved spectroscopy (PRESS) or the stimulated echo acquisition mode (STEAM), and a 3 x 3 x 3 cm volume of interest was positioned at the hippocampus. N-Acetyl-aspartate (NAA) and choline-containing substance (Cho) signals were evaluated. The results showed decreased NAA and elevated Cho or asymmetry of both NAA and Cho in the epileptogenic focus in 19/20 cases. The reductions in NAA presumably reflect neuronal loss, while the elevation of Cho probably represents membrane break down within the lesion. These abnormalities were observed in 19/20 cases (95%), whereas abnormal magnetic resonance imaging was detected in only 6/20 (30%) of the patients. Thus, 1H-MRS appears to be a useful, non - invasive modality for evaluating metabolic changes in epileptogenic foci, and these metabolic changes can serve as a more sensitive indicator than magnetic resonance imaging.
  • 川口進, 鎌田恭輔, 板本孝治, 大槻浩之, 磯西克佳, 中山若樹, 下山三夫, 徳田耕一, 宝金清博
    Brain Nerve 47 1 43-48  1995年01月 [査読無し][通常論文]
  • K KAMADA, K HOUKIN, Y IWASAKI, H ABE
    BRAIN EDEMA IX 60 491 - 493 1994年 [査読有り][通常論文]
  • K. Kamada, O. Oshiro, F. Takeuchi, S. Kuriki, K. Houkin, H. Abe
    Brain and Nerve 44 3 265 - 270 1992年 [査読有り][通常論文]
  • T NAKADA, K HOUKIN, K HIDA, IL KWEE
    MAGNETIC RESONANCE IN MEDICINE 18 1 9 - 14 1991年03月 [査読有り][通常論文]
  • 中川 翼, 大里 孝夫, 布村 充, 井原 博, 多田 光宏, 小林 延光, 宝金 清博, 井須 豊彦
    脳卒中の外科 19 1 143 - 147 一般社団法人 日本脳卒中の外科学会 1991年 
    Temporary clipping of the parent artery in acute-stage aneurysm surgery was popularized by the late Prof. Jiro Suzuki. However, it is not rare that the internal carotid artery (ICA) proximal to the aneurysm cannot easily be identified even during surgery for ICA aneurysms of the common portion. In addition, the difficulty of identification of the ICA proximal to the aneurysm is not always predicted before the operation.
    In this paper, authors emphasize that exposure of the common carotid artery in the neck of patients is highly recommended even in aneurysm surgery of the ICA (excluding the cavernous or opthalmic portion) in the cases shown below;
    (1) when a ruptured aneurysm protrude posterioly, is located dorsally, or shows up small or large on the angiogram.
    (2) when it is not possible to separate the aneurysm from the posterior communicating or anterior choroidal artery on the angiogram.
    (3) when younger neurosurgeons perform the operation.
  • 村田 純一, 上野 一義, 小柳 泉, 宝金 清博, 多田 光宏
    医療 40 5 400 - 403 Japanese Society of National Medical Services 1986年 
    甲状腺癌はしばしば血行性転移を来すが, 頭蓋骨転移はまれである. 今回我々は甲状腺腫瘍の手術をしてから8年後に後頭部の皮下腫瘤に気づき, 翌年当科にて頭蓋骨腫瘍の診断で腫瘤を摘出し, 病理学的に甲状腺癌の転移と診断した1例を経験したので報告する.
    患者は64才女性. 昭和50年に甲状腺片葉摘除術を受けている. 58年6月ころ, 後頭部の皮下腫瘤に気づき, 59年9月当科受診した. 神経学的陽性所見はなく, 頭部単純写にて後頭部正中に径8cm, 左前頭部に径1cmの骨欠損を認めた. 放射線学的に脳実質内に異常を認めなかつた. 外頸動脈のembolizationを行い, 翌日後頭部の腫瘍を全摘した. 病理診断は甲状腺濾胞癌の転移であつた.
    文献上, 甲状腺癌の頭蓋骨転移で大きな腫瘤を形成した症例は少なく, これを全摘しえた例は本症例が初であると思われる.
  • Spinal epidural and subdural hemorrhage in hemophilia A. Case report
    K. Houkin, H. Abe, Y. Iwasaki
    Nervous System in Children 10 1 1 - 5 1985年 
    The authors described a case of epidural and subdural hemorrhage of a 9-month-old boy with hemophilia A. This patient, 9-month-old boy, was admitted to a pediatric hospital with a complaint moderate fever and irritability. He fell down and had mild trauma to his back one week previous to admission but he was asymptomatic for 2 days after the episode. Lumbar puncture performed on admission revealed a slightly xanthochromatic fluid with high protein content, which suggested a mild hemorrhage. Next lumbar puncture was performed 8 days after the admission but after lumbar paraparesis of the bilateral lower limbs and urinary incontinence appeared gradually. The laboratory examinations showed a prolonged partial thromboplastin time and decreased activity of VIII factor, and the substitution therapy of VIII factor was performed. Spinal high resulutions CT scan showed a epidural hematoma from T-8 to cauda equina and increased density of subdural space suggesting a subdural hematoma. Fortunately the symptoms improved gradually and he could move his legs about 10 days after the onset of the symptoms. Follow-up CT scan revealed a resolving of the hematoma. Intra-cranial hemorrhage with hemophilia has been discussed in many reports but hemorrhage in the spinal canal has been rarely reported. Case reports of hemorrhage in the spinal canal with hemophilia were reviewed and a clinical problem accompanied with the lumbar puncture to a patient with hemophilia and therapies for this hemorrhage were discussed.

MISC

  • 伊東雅基, 内野晴登, 東海林菊太郎, 杉山拓, 川堀真人, 岩田育子, 矢部一郎, 寶金清博, 藤村幹 月刊メディカル・サイエンス・ダイジェスト 50 (6) 2024年
  • 伊東雅基, 川堀真人, 杉山拓, 東海林菊太郎, 舘澤諒大, 内野晴登, 数又研, 寳金清博, 藤村幹 Mt. Fuji Workshop on CVD 39th 2022年
  • 伊東雅基, 内野晴登, 川堀真人, 杉山拓, 数又研, 宝金清博, 藤村幹 日本分子脳神経外科学会プログラム・抄録集 22nd 2022年
  • 江口 克紀, 白井 慎一, 松島 理明, 加納 崇裕, 山崎 和義, 濱内 祝嗣, 笹森 徹, 関 俊隆, 北川 まゆみ, 大槻 美佳, 寳金 清博, 佐々木 秀直, 矢部 一郎 パーキンソン病・運動障害疾患コングレスプログラム・抄録集 15回 75 -75 2021年07月
  • 寳金 清博 学術の動向 = Trends in the sciences 26 (6) 24 -27 2021年06月
  • 杉山 拓, 寳金 清博 日本医事新報 (5067) 38 -39 2021年06月
  • 江口 克紀, 矢部 一郎, 白井 慎一, 松島 理明, 加納 崇裕, 山崎 和義, 濱内 祝嗣, 笹森 徹, 関 俊隆, 北川 まゆみ, 大槻 美佳, 寳金 清博, 佐々木 秀直 パーキンソン病・運動障害疾患コングレスプログラム・抄録集 14回 83 -83 2021年02月
  • 伊東雅基, 川堀真人, 杉山拓, 東海林菊太郎, 舘澤諒大, 内野晴登, 数又研, 寳金清博, 藤村幹 脳循環代謝(Web) 33 (1) 2021年
  • 杉山拓, 寳金清博 週刊日本医事新報 (5067) 2021年
  • 江口 克紀, 白井 慎一, 山崎 和義, 濱内 祝嗣, 松島 理明, 加納 崇裕, 笹森 徹, 平田 健司, 関 俊隆, 志賀 哲, 大槻 美佳, 寳金 清博, 佐々木 秀直, 矢部 一郎 日本定位・機能神経外科学会プログラム・抄録集 60回 97 -97 2020年12月
  • 川堀 真人, 七戸 秀夫, 黒田 敏, 寳金 清博 脳神経外科ジャーナル 29 (11) 777 -783 2020年11月 
    脳梗塞・頭部外傷・脊髄損傷・パーキンソン病など中枢神経疾患に対する細胞治療・再生医療が、「基礎研究」の段階から、臨床応用に向けた「治験」さらには「保険診療」の段階に入ってきている。これらの成績は既存治療では成し得なかった中枢神経を再生ないし回復させる治療法として、十分期待できるものである。自家骨髄幹細胞の「ステミラック注」が脊髄損傷の亜急性期患者に対して期限つき条件つき承認で保険適応となり、中枢神経疾患に対する初めての「再生医療等製品」となった。また、脳梗塞においても複数の治験が進行中でその結果が待たれる。より効果の高い細胞の開発、投与方法、投与時期、医療費削減、効果が高い患者群の決定など残された課題も多いが、基礎研究および臨床経験の蓄積など今後の発展に期待したい。(著者抄録)
  • 小山 雅之, 樋室 伸顕, 佐藤 琢真, 簑島 暁帆, 竹内 利治, 橋本 暁佳, 齋藤 重幸, 三浦 哲嗣, 寳金 清博, 大西 浩文 日本循環器病予防学会誌 55 (3) 224 -224 2020年11月
  • DeFrictorによる経動脈的塞栓術についての検討
    長内 俊也, 東海林 菊太郎, 今井 哲秋, 杉山 拓, 中山 若樹, 数又 研, 寳金 清博 脳血管内治療 5 (Suppl.) 68 -68 2020年11月
  • 江口 克紀, 矢部 一郎, 白井 慎一, 山崎 和義, 濱内 祝嗣, 松島 理明, 加納 崇裕, 笹森 徹, 関 俊隆, 大槻 美佳, 寳金 清博, 佐々木 秀直 臨床神経生理学 48 (5) 507 -507 2020年10月
  • 江口 克紀, 矢部 一郎, 白井 慎一, 山崎 和義, 濱内 祝嗣, 松島 理明, 加納 崇裕, 笹森 徹, 関 俊隆, 大槻 美佳, 寳金 清博, 佐々木 秀直 臨床神経生理学 48 (5) 534 -534 2020年10月
  • 後藤 秀輔, 長内 俊也, 牛越 聡, 栗栖 宏多, 下田 祐介, 寳金 清博 Neurological Surgery 48 (4) 335 -340 2020年04月 [査読無し][通常論文]
     
    40代男性。幼少期の転倒を原因とする顎変形症および咬合不全に対するLe Fort I型骨切り術、下顎枝矢状分割術が行われ、翌々日に拍動性の耳鳴りを自覚した。MRI/MRA、血管造影所見より、顎動脈の動静脈瘻(maxillary AVF)と診断し、血管内治療を行った。手術は、シャント周囲の血管が蛇行し血管径が細いため、シャント部位の直近までマイクロカテーテルを誘導してNBCA注入を行って塞栓した。術直後より耳鳴りは消失し、新たな神経症状や周術期合併症は認めなかった。本症例は顎動脈の1st segment、pterygoid plexusのAVFで、手術操作による引き抜き損傷などが発生機序と考えられた。
  • 川堀 真人, 七戸 秀夫, 黒田 敏, 寳金 清博 臨床画像 36 (4月増刊) 141 -150 2020年04月 [査読無し][通常論文]
     
    ＜文献概要＞脳梗塞・頭部外傷・脊髄損傷・Parkinson病など中枢神経疾患に対する細胞治療・再生医療が,基礎研究から治験の段階(bench to bed)に入っている。これらは既存治療ではなしえなかった中枢神経を再生・回復させる治療法として大きく期待されている。本報告では,脳血管障害に対する研究結果および今後の課題について概説する。
  • 後藤 秀輔, 長内 俊也, 牛越 聡, 栗栖 宏多, 下田 祐介, 寳金 清博 Neurological Surgery 48 (4) 335 -340 2020年04月 [査読無し][通常論文]
  • 澤谷 亮佑, 山口 秀, 伊師 雪友, 茂木 洋晃, 小林 浩之, 杉山 未奈子, 長 祐子, 井口 晶裕, 大塚 拓也, 今本 鉄平, 高桑 恵美, 寳金 清博 脳神経外科速報 30 (3) 300 -305 2020年03月 [査読無し][通常論文]
     
    15歳男。繰り返す嘔吐を主訴に、近医にて松果体部腫瘍とそれに伴う閉塞性水頭症が認められた。急速に進行する意識障害を呈し、脳室ドレナージ術が施行され、当院転院となった。画像所見では、松果体部に充実性腫瘍性病変を認め、右側脳室前角や左側脳室体部、鞍上部にも造影病変を認め、播種性病変と考えられた。ドレーンを留置し、意識障害は改善した。生検にてgerminomaであったが、血液検査および画像所見から臨床的にyolk sac tumor(YST)が主体であるmixed germ cell tumor(GCT)と診断した。ICE(IFOS+CDDP+VP-16)化学療法を開始したが、意識レベルが低下し、腫瘍嚢胞性病変の増大を顕著に認めた。嚢胞性病変増大による脳幹や視床圧迫が意識障害の主体と考え、嚢胞の開窓を行い、脳幹圧迫を解除した。第三脳室および右側脳室体部から嚢胞壁を穿刺すると、黄色の内容物が流出した。また、前回認められた灰白隆起部分の播種性病変は消失していたため、第三脳室底開窓術も施行した。前回生検標本との結果を踏まえ、YST主体のmixed GCTと診断した。術後は経過良好で、意識状態は改善した。腫瘍の縮小を認め、腫瘍を全摘出した。術後ICE療法を行い、初回治療から7ヵ月経過したが、腫瘍再発は認められなかった。
  • 川堀 真人, 寳金 清博 医学のあゆみ 272 (10) 1044 -1050 2020年03月 [査読無し][通常論文]
     
    いったん障害を受けると回復することがないといわれてきた脳・脊髄の病気に対する再生医療の期待は大きい。実際、脳梗塞、頭部外傷、脊髄損傷、パーキンソン病などに対する再生医療・細胞治療が、"基礎研究"の段階から臨床応用に向けた"治験"、さらに一部は"先進医療・保険診療"の段階に入ってきている。これらの多くが間葉系幹細胞を用いており、その安全性・有用性が注目されている。2019年には、自家骨髄幹細胞の"ステミラック注"が脊髄損傷の亜急性期患者に対して期限および条件付き承認で保険適用となり、中枢神経疾患に対するはじめての"再生医療等製品"となった。また、脳梗塞に対する細胞治療も複数の治験が進行中で、その結果が待たれる。本稿では、間葉系幹細胞を用いた中枢神経疾患に対する細胞治療・再生医療の作用機序、すでに報告されている治験および現在進行中の研究内容、および残された課題について概説する。(著者抄録)
  • 化学療法導入直後に急激な嚢胞性増大を示した卵黄嚢腫瘍主体の頭蓋内胚細胞腫瘍の1例
    澤谷 亮佑, 山口 秀, 伊師 雪友, 茂木 洋晃, 小林 浩之, 杉山 未奈子, 長 祐子, 井口 晶裕, 大塚 拓也, 今本 鉄平, 高桑 恵美, 寳金 清博 脳神経外科速報 30 (3) 300 -305 2020年03月 [査読無し][通常論文]
  • 中村 伸理子, 有賀 徹, 寳金 清博, 嘉山 孝正, 橋本 重厚, 堤 晴彦, 中島 勧, 岩瀬 博太郎, 米村 滋人, 櫻井 淳, 三木 保, 吉田 謙一, 大嶽 浩司, 小林 弘幸, 坂本 哲也, 大磯 義一郎, 中島 和江, 水谷 渉, 全国医学部長病院長会議大学病院の医療事故対策委員会 日本医師会雑誌 148 (11) 2213 -2218 2020年02月 [査読無し][通常論文]
     
    医療事故調査制度に関する大学病院と都道府県医師会との連携、大学病院の支援状況および課題等を把握することを目的に、全国の大学病院に対しアンケート調査を行った。全国80大学病院(本院)および各大学病院から情報を得た分院57施設に対し、記名式でメールにて回答を求めた。アンケートでは平成30年度の実績と平成31年4月時点の状況を尋ねた。平成31年は「支援手順等が決まり支援提供」が本院61大学(76%)、分院10施設(23%)、「支援手順等が決まった」が本院13大学(16%)、分院4施設(9%)であり、「連絡協議会等を行っていない」は本院該当なし、分院28施設(65%)であった。大学病院の支援体制に関して、「対応可能」支援は「相談」は本院64大学(80%)、分院17施設(40%)、「解剖」は本院70大学(88%)、分院18施設(42%)、「死亡時画像診断」は本院46大学(58%)、分院15施設(35%)、「外部委員」は本院76大学(95%)、分院17施設(40%)、「報告書の助言やチェック」は本院58大学(73%)、分院17施設(40%)であった。
  • 小林浩之, 寺坂俊介, 茂木洋晃, 山口秀, 伊師雪友, 浅岡克行, 宝金清博, 宝金清博 日本間脳下垂体腫瘍学会プログラム・抄録集 30th 2020年
  • 川堀 真人, 七戸 秀夫, 黒田 敏, 寳金 清博 神経治療学 37 (3) 386 -390 2020年 

    Stem cell therapy for central nervous system disorders have launched from basic research to the clinical trial phase. Many clinical trials are currently executed in Japan including ischemic stroke, traumatic brain injury, Parkingson's disease, and spinal cord injury. The preliminary data from these trials are promising and there is a strong expectation both from the medical and patient point of view. However, there are still so many unsolved questions which require further experimental procedures. The questions include cell types, transplantation route, transplantation timing and cell dosages. This article will highlight the basic knowledge of stem cell and the preliminary result of clinical trials launched in Japan.

  • 江口克紀, 矢部一郎, 白井慎一, 山崎和義, 濱内祝嗣, 松島理明, 加納崇裕, 笹森徹, 関俊隆, 大槻美佳, 寳金清博, 佐々木秀直 臨床神経生理学(Web) 48 (5) 2020年
  • 江口克紀, 矢部一郎, 白井慎一, 山崎和義, 濱内祝嗣, 松島理明, 加納崇裕, 笹森徹, 関俊隆, 大槻美佳, 寳金清博, 佐々木秀直 臨床神経生理学(Web) 48 (5) 2020年
  • 長内俊也, 東海林菊太郎, 今井哲秋, 杉山拓, 中山若樹, 数又研, 寳金清博 脳血管内治療(Web) 5 (Supplement) 2020年
  • 岡本迪成, 山口秀, 伊師雪友, 茂木洋晃, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍学会プログラム・抄録集 38th 2020年
  • 川堀真人, 七戸秀夫, 黒田敏, 寳金清博 神経治療学(Web) 37 (3) 2020年
  • 濱内 祝嗣, 長内 俊也, 関 俊隆, 飛騨 一利, 宝金 清博 脊髄外科 33 (3) 309 -312 2019年12月 [査読無し][通常論文]
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  • 川堀 真人, 七戸 秀人, 黒田 敏, 寳金 清博 神経治療学 36 (6) S128 -S128 2019年10月 [査読無し][通常論文]
  • 川堀 真人, 寳金 清博 脳血管病Trend Review 1 (1) 28 -31 2019年09月 [査読無し][通常論文]
     
    脳梗塞に対する幹細胞を用いた再生医療の現状とその課題および将来像について概説した。現在多くの基礎および臨床研究が進められている細胞種として、胚性幹細胞(ES細胞)、人工多能性幹細胞(iPS細胞)、体性幹細胞(間葉系幹細胞、神経幹細胞等)などがあげられるが、特に臨床応用が進んでいるのが骨髄由来の間葉系幹細胞(BMSC)である。幹細胞の作用機序として、分化による直接効果と保護効果による間接効果が考えられる。現在、脳梗塞に対して幹細胞を用いた臨床試験がおこなわれているが、最適な細胞種類、細胞数、投与ルート、投与時期、患者群など解決されていない問題も残されている。
  • 川堀 真人, 寳金 清博 脳血管病Trend Review 1 (1) 28 -31 2019年09月 [査読無し][通常論文]
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  • 茂木洋晃, 山口秀, 岡本迪成, 伊師雪友, 寺坂俊介, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 24th 131 2019年08月30日 [査読無し][通常論文]
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    寳金 清博, 伊東 雅基 日本医師会雑誌 148 (特別1) S79 -S81 2019年06月 [査読無し][通常論文]
  • 刺激装置の変更により脳深部刺療法による開眼失行が改善したパーキンソン病の1例
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    医療事故調査制度への大学病院の対応を調べるため、全国80大学病院を対象とするアンケート調査を実施した。今回の調査では大学病院の本院を介して分院にも回答を求めた。本院の「対応可能」支援は「相談」69大学(86%)、「解剖」72大学(90%)、「死亡時画像診断(Ai)」46大学(58%)、「専門家派遣」77大学(96%)、「報告書の助言やチェック」60大学(76%)であった。分院の「対応可能」支援は「相談」19病院(53%)、「解剖」16病院(44%)、「Ai」15病院(42%)、「専門家派遣」19病院(53%)、「報告書の助言やチェック」18病院(50%)であった。支援症例の医師会との連携に関して、支援提供に当たり「医師会を通さず」は本院17症例(13%)、分院6症例(33%)、「全体的医師会主導」または「部分的医師会主導」は本院76症例(57%)、分院12症例(67%)、「必要時医師会連絡」は本院40症例(30%)、分院なしであった。
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    次第に悪化する短期記憶障害の進行および性格変化で発症した59歳女性。前頭蓋底の嚢胞性病変と脳室内・くも膜下腔に散在する脂肪滴を認め、破裂した類皮嚢胞と術前診断した。開頭にて嚢胞開窓と嚢胞内容摘出を施行したところ、嚢胞内に充実性病変が確認され、組織学的に外胚葉、中胚葉、内胚葉のすべての分化した成分から構成され、成熟奇形腫と診断された。成熟奇形腫の破裂はきわめてまれとされ、文献的考察も含め、その特徴について考察する。(著者抄録)
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  • 伊師雪友, 関俊隆, 高宮宗一郎, 山崎和義, 濱内祝嗣, 山口秀, 寶金清博, 寶金清博, 石田雄介, 小田義崇, 飛騨一利 日本脊髄外科学会プログラム・抄録集 34th 2019年
  • 茂木洋晃, 山口秀, 伊師雪友, 遠藤将吾, 小林浩之, 寺坂俊介, 鈴木正宣, 中丸裕爾, 本間明宏, 宝金清博 日本脳腫瘍学会プログラム・抄録集 37th 2019年
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    小林 聡, 長内 俊也, 中山 若樹, 鐙谷 武雄, 数又 研, 藤間 憲幸, 清水 幸衣, 大野 浩太, 宝金 清博 脳血管内治療 3 (Suppl.) S268 -S268 2018年11月 [査読無し][通常論文]
  • 頭蓋内硬膜動静脈瘻に対する4D-MRAの検討
    東海林 菊太郎, 長内 俊也, 藤間 憲幸, 牛越 聡, 寳金 清博 脳血管内治療 3 (Suppl.) S203 -S203 2018年11月 [査読無し][通常論文]
  • 白枝 友子, 新明 康弘, 和田 莉奈, 鈴木 佳代, 中村 佳代子, 杉山 拓, 寳金 清博, 陳 進輝, 石田 晋 神経眼科 35 (増補1) 75 -75 2018年11月 [査読無し][通常論文]
  • 脳梗塞急性期に対する自家骨髄間質細胞(BMSC)移植治療 医師主導治験RAINBOW研究
    川堀 真人, 七戸 秀夫, 山崎 和義, 黒田 敏, 寳金 清博 脳循環代謝 30 (1) 66 -66 2018年10月 [査読無し][通常論文]
  • Brain Japanがめざす脳卒中治療への取り組み 脳梗塞に対する細胞療法
    寳金 清博, 川堀 真人, 七戸 秀夫 脳循環代謝 30 (1) 71 -71 2018年10月 [査読無し][通常論文]
  • ヘモグロビン-アルブミン複合体HemoActによる脳虚血再灌流急性期治療の開発
    月花 正幸, 鐙谷 武雄, 小松 晃之, 船木 亮佑, 栗栖 宏太, 新保 大輔, 伊藤 康裕, 中山 若樹, 数又 研, 寳金 清博 脳循環代謝 30 (1) 115 -115 2018年10月 [査読無し][通常論文]
  • Brain Japanがめざす脳卒中治療への取り組み 脳梗塞に対する細胞療法
    寳金 清博, 川堀 真人, 七戸 秀夫 脳循環代謝 30 (1) 71 -71 2018年10月 [査読無し][通常論文]
  • 月花正幸, 鐙谷武雄, 小松晃之, 船木亮佑, 栗栖宏太, 新保大輔, 伊藤康裕, 中山若樹, 数又研, 寳金清博 脳循環代謝(Web) 30 (1) 115 -115 2018年10月 [査読無し][通常論文]
  • 川堀真人, 七戸秀夫, 山崎和義, 黒田敏, 寳金清博 脳循環代謝(Web) 30 (1) 66 -66 2018年10月 [査読無し][通常論文]
  • 山口秀, 小林浩之, 伊師雪友, 茂木洋晃, 岡本迪成, 越前谷すみれ, 寺坂俊介, 寺坂俊介, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 23rd 123 2018年09月01日 [査読無し][通常論文]
  • 岡本迪成, 山口秀, 茂木洋晃, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 23rd 140 2018年09月01日 [査読無し][通常論文]
  • 中村伸理子, 有賀徹, 寳金清博, 橋本重厚, 堤晴彦, 中島勧, 深山正久, 吉田謙一, 大嶽浩司, 小林弘幸, 坂本哲也, 大磯義一郎, 中島和江, 太田吉夫, 山下裕一, 坂梨又郎, 水谷渉, 嘉山孝正 日本医師会雑誌 147 (6) 1254‐1258 -1258 2018年09月01日 [査読無し][通常論文]
     
    医療事故調査制度に関する大学病院と都道府県医師会との連携、大学病院の支援状況等を把握することを目的としてアンケート調査を行った。全国80大学病院に対し記名式でメールにて回答を求めた。平成29年3月までに「支援手順等が決まり支援提供」は47大学(59%)、「支援手順等が決まった」は25大学(31%)であり、「連絡協議会等は未了」は該当なしであった。大学病院の支援状況では、対応可能な支援は「相談」73大学(92%)、「解剖」75大学(94%)、「死亡時画像診断(Ai)」45大学(57%)、「専門家派遣」78大学(98%)、「報告書の助言やチェック」66大学(85%)であった。他方、「原則不可」は「Ai」が34大学(43%)と最も多かった。5項目すべての支援にシステムを整備し対応可能である大学は16大学(20%)、Aiを除く4項目以上に対応可能である大学は61大学(76%)であった。
  • 寳金 清博 最新医療経営 : phase3 : フェイズ・スリー (407) 1 -3 2018年07月
  • 脳深部刺激療法術後2年間における15例の神経心理学的検討
    白井 慎一, 矢部 一郎, 山崎 和義, 濱内 祝嗣, 松島 理明, 加納 崇裕, 笹森 徹, 関 俊隆, 北川 まゆみ, 大槻 美佳, 寳金 清博, 佐々木 秀直 パーキンソン病・運動障害疾患コングレスプログラム・抄録集 12回 82 -82 2018年07月 [査読無し][通常論文]
  • 白井慎一, 矢部一郎, 山崎和義, 濱内祝嗣, 松島理明, 加納崇裕, 笹森徹, 関俊隆, 北川まゆみ, 大槻美佳, 寳金清博, 佐々木秀直 パーキンソン病・運動障害疾患コングレスプログラム・抄録集 12th 82 -82 2018年07月 [査読無し][通常論文]
  • GPi-DBSが奏効した遅発性ジスキネジアの1例
    佐藤 翔紀, 白井 慎一, 松島 理明, 矢部 一郎, 加納 崇裕, 北川 まゆみ, 山崎 和義, 濱内 祝嗣, 笹森 徹, 関 俊隆, 寳金 清博, 佐々木 秀直 臨床神経学 58 (5) 359 -359 2018年05月 [査読無し][通常論文]
  • 中国における中枢神経疾患に対する細胞治療の最新動向
    王 子ほう, 譚 成博, 川堀 真人, 七戸 秀夫, 寶金 清博 北海道医学雑誌 93 (1) 13 -20 2018年05月 [査読無し][通常論文]
     
    オンライン上で、中枢神経再生の臨床試験に関する中国発の学術論文を検索した。網羅的に中枢神経系再生に関する臨床試験の論文は、英語379報、中国語3795報であった。中国発の幹細胞研究に関する臨床試験数は2012年初頭まで98であったが、2015年には244まで増加し、18試験が中枢神経系疾患に関する試験であった。中国において2015年末までに承認された幹細胞製品は10製品認め、5製品は中国の政府機関が申請しており、残りの5製品は中国企業によるものであった。最近(2015年7月20日)、中国国家食品薬品監督管理局(CFDA)が「幹細胞臨床研究管理方法(試行)」を発布したが、この法律が中国における幹細胞の管理方法に関する最初の規範となった。さらに2015年7月31日には、CFDAが「幹細胞製剤の品質管理及び臨床前指導原則」を発布している。
  • 王 子ほう, 譚 成博, 川堀 真人, 七戸 秀夫, 寶金 清博 北海道医学雑誌 93 (1) 13 -20 2018年05月 [査読無し][通常論文]
     
    オンライン上で、中枢神経再生の臨床試験に関する中国発の学術論文を検索した。網羅的に中枢神経系再生に関する臨床試験の論文は、英語379報、中国語3795報であった。中国発の幹細胞研究に関する臨床試験数は2012年初頭まで98であったが、2015年には244まで増加し、18試験が中枢神経系疾患に関する試験であった。中国において2015年末までに承認された幹細胞製品は10製品認め、5製品は中国の政府機関が申請しており、残りの5製品は中国企業によるものであった。最近(2015年7月20日)、中国国家食品薬品監督管理局(CFDA)が「幹細胞臨床研究管理方法(試行)」を発布したが、この法律が中国における幹細胞の管理方法に関する最初の規範となった。さらに2015年7月31日には、CFDAが「幹細胞製剤の品質管理及び臨床前指導原則」を発布している。
  • GPi-DBSが奏効した遅発性ジスキネジアの1例
    佐藤 翔紀, 白井 慎一, 松島 理明, 矢部 一郎, 加納 崇裕, 北川 まゆみ, 山崎 和義, 濱内 祝嗣, 笹森 徹, 関 俊隆, 寳金 清博, 佐々木 秀直 臨床神経学 58 (5) 359 -359 2018年05月 [査読無し][通常論文]
  • 七戸 秀夫, 川堀 真人, 寳金 清博 レギュラトリーサイエンス学会誌 8 (2) 115 -121 2018年05月 [査読無し][通常論文]
     
    脳梗塞に対する細胞治療が国内外で臨床試験として開始されつつある。2012年度に、われわれは「脳梗塞の再生医療」に関して厚生労働省から革新的医薬品・医療機器・再生医療製品実用化促進事業実施機関に選定された。本事業のもとで、われわれは自家骨髄間質細胞(bone marrow stromal cells:BMSC)移植による脳梗塞再生治療の医師主導治験(第I相:RAINBOW研究)を準備し、2017年6月に最初の被験者が登録された。本治験は、(1)ウシ胎仔血清(FBS)などの代替として、他家ヒト血小板溶解物(platelet lysate:PL)を添加し、自家BMSCを培養する、(2)脳梗塞周辺部へ脳定位的手術により細胞を直接移植する、(3)MRIによる移植細胞の挙動把握を目的とし、超常磁性酸化鉄(SPIO)製剤によりBMSCをラベルする、(4)FDG-PETやIomazenil-SPECTを用いて、細胞移植がホスト脳に及ぼす影響を評価するなど、過去の臨床試験と異なる新規性のあるプロトコルを採用し、われわれは第2世代の臨床試験と自負している。本稿ではBMSC移植治療の作用機序と治療戦略、非臨床試験、治験プロトコルの観点から、われわれの経験を報告する。(著者抄録)
  • 高宮宗一朗, 関俊隆, 山崎和義, 濱内祝嗣, 寺坂俊介, 寳金清博 小児の脳神経 43 (2) 192 -192 2018年04月30日 [査読無し][通常論文]
  • 関俊隆, 飛騨一利, 山崎和義, 寳金清博 脊髄外科 32 (1) 80‐81 -81 2018年04月25日 [査読無し][通常論文]
     
    小児無症候性TCS(脊髄係留症候群)に対して手術治療を行った14例(男児9例、女児5例、初回手術時平均年齢28.6ヵ月)の治療成績について検討した。その結果、初回手術後94ヵ月目までは全例が無症候の状態であったが、その後、2例(14.3%)に遅延性神経障害を認め、そのうち1例に対して再係留解除術を行った。本検討における年間悪化率は1.8%であり、無症候性脊髄脂肪腫に対して保存療法を行ったKulkarniらやWykesらの遅発性神経障害の年間発生率3.7〜4%と比べても遜色のない成績であった。
  • 生下時に認めた脊髄髄膜瘤の術後3年目に脊髄癒着性くも膜炎をきたし、再手術後、短期間に再発した幼児例
    関 俊隆, 山崎 和義, 池田 拓磨, 岡本 迪成, 森島 穣, 宝金 清博 小児の脳神経 43 (2) 219 -219 2018年04月 [査読無し][通常論文]
  • 再生医療と病理 脳梗塞急性期に対する自家骨髄間質細胞(BMSC)移植治療 医師主導治験RAINBOW研究
    川堀 真人, 七戸 秀夫, 山崎 和義, 黒田 敏, 寺坂 俊介, 寶金 清博 日本病理学会会誌 107 (1) 230 -230 2018年04月 [査読無し][通常論文]
  • 関 俊隆, 飛騨 一利, 山崎 和義, 寳金 清博 脊髄外科 32 (1) 80 -81 2018年04月 [査読無し][通常論文]
  • 脊髄髄膜瘤の特徴を有した脊髄嚢胞瘤の1例
    高宮 宗一朗, 関 俊隆, 山崎 和義, 濱内 祝嗣, 寺坂 俊介, 寳金 清博 小児の脳神経 43 (2) 192 -192 2018年04月 [査読無し][通常論文]
  • 関 俊隆, 浜内 祝嗣, 山崎 和義, 宝金 清博 Journal of Spine Research 9 (3) 325 -325 2018年03月 [査読無し][通常論文]
  • 川堀 真人, 七戸 秀夫, 黒田 敏, 宝金 清博 日独医報 62 (2) 161 -167 2018年01月 [査読無し][通常論文]
     
    特集 脳梗塞超急性期診療 7. 出版年次: 2017
  • 寶金 清博, 冨永 悌二, 宮本 享, 鈴木 則宏, 黒田 敏, 小泉 昭夫, 高橋 淳, 佐藤 典宏, 数又 研, 高木 康志, 大木 宏一, 舟木 健史, 藤村 幹, 厚生労働科学研究費補助金難治性疾患克服事業ウイリス動脈輪閉塞症における病態・治療に関する研究班 脳卒中の外科 46 (1) 1 -24 2018年01月 [査読無し][通常論文]
  • 山口秀, 小林浩之, 茂木洋晃, 岡本迪成, 伊師雪友, 越前谷すみれ, 寺坂俊介, 寳金清博 日本脳腫瘍学会プログラム・抄録集 36th 103 2018年 [査読無し][通常論文]
  • 伊師雪友, 山口秀, 茂木洋晃, 岡本迪成, 越前谷すみれ, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍学会プログラム・抄録集 36th 83 2018年 [査読無し][通常論文]
  • 佐藤翔紀, 白井慎一, 松島理明, 矢部一郎, 加納崇裕, 北川まゆみ, 山崎和義, 浜内祝嗣, 笹森徹, 関俊隆, 寳金清博, 佐々木秀直 臨床神経学(Web) 58 (5) 359(J‐STAGE) 2018年 [査読無し][通常論文]
  • 寳金清博, 川堀真人, 七戸秀夫 脳循環代謝(Web) 30 (1) 71 2018年 [査読無し][通常論文]
  • 寳金清博 日本ニューロリハビリテーション学会学術集会プログラム・抄録集 9th 43 2018年 [査読無し][通常論文]
  • 七戸秀夫, 七戸秀夫, 川堀真人, 寳金清博 日本再生医療学会総会(Web) 17th ROMBUNNO.O‐51‐6 (WEB ONLY) 2018年 [査読無し][通常論文]
  • 浜内祝嗣, 長内俊也, 関俊隆, 川堀真人, 岡本迪成, 飛騨一利, 寺坂俊介, 寳金清博 日本脊髄外科学会プログラム・抄録集 33rd 310 2018年 [査読無し][通常論文]
  • 七戸秀夫, 川堀真人, 寳金清博 レギュラトリーサイエンス学会誌 8 (2) 115‐121(J‐STAGE) -121 2018年 [査読無し][通常論文]
     

    脳梗塞に対する細胞治療が国内外で臨床試験として開始されつつある. 2012年度に, われわれは ｢脳梗塞の再生医療｣ に関して厚生労働省から革新的医薬品・医療機器・再生医療製品実用化促進事業実施機関に選定された. 本事業のもとで, われわれは自家骨髄間質細胞 (bone marrow stromal cells: BMSC) 移植による脳梗塞再生治療の医師主導治験 (第Ⅰ相 : RAINBOW研究) を準備し, 2017年6月に最初の被験者が登録された. 本治験は, ①ウシ胎仔血清 (FBS) などの代替として, 他家ヒト血小板溶解物 (platelet lysate: PL) を添加し, 自家BMSCを培養する, ②脳梗塞周辺部へ脳定位的手術により細胞を直接移植する, ③MRIによる移植細胞の挙動把握を目的とし, 超常磁性酸化鉄 (SPIO) 製剤によりBMSCをラベルする, ④FDG-PETやIomazenil-SPECTを用いて, 細胞移植がホスト脳に及ぼす影響を評価するなど, 過去の臨床試験と異なる新規性のあるプロトコルを採用し, われわれは第2世代の臨床試験と自負している. 本稿ではBMSC移植治療の作用機序と治療戦略, 非臨床試験, 治験プロトコルの観点から, われわれの経験を報告する.

  • 鐙谷武雄, 月花正幸, 小松晃之, 船木亮佑, 川堀真人, 長内俊也, 中山若樹, 数又研, 寳金清博 人工血液 25 (1) 26 2017年11月30日 [査読無し][通常論文]
  • もやもや病の初回手術不応例に対するrepeat bypassの効果
    内野 晴登, 山本 修輔, 柏崎 大奈, 秋岡 直樹, 宝金 清博, 黒田 敏 脳循環代謝 29 (1) 154 -154 2017年11月 [査読無し][通常論文]
  • 硬膜動静脈瘻に対する経動脈的塞栓術 Onyxの適応と各種adjunctive techniqueについて
    浅野 剛, 松田 達磨, 久保田 俊介, 荷堂 謙, 三ツ橋 茂雄, 山上 岩男, 景山 雄介, 櫻井 寿郎, 瀧澤 克己, 宝金 清博 脳血管内治療 2 (Suppl.) S111 -S111 2017年11月 [査読無し][通常論文]
  • 脳梗塞に対する自家骨髄間質細胞移植 RAINBOW研究
    川堀 真人, 七戸 秀夫, 寺坂 俊介, 黒田 敏, 宝金 清博 脳循環代謝 29 (1) 158 -158 2017年11月 [査読無し][通常論文]
  • 頸動脈直接穿刺を行った血栓回収療法の3例
    川堀 真人, 長内 俊也, 新谷 好正, 岩崎 素之, 馬渕 正二, 宝金 清博 脳血管内治療 2 (Suppl.) S305 -S305 2017年11月 [査読無し][通常論文]
  • 急性期虚血病態を治す/急性期脳虚血病態を治療する 脳虚血再灌流時の微小循環障害にターゲットをおいた脳保護療法の開発
    鐙谷 武雄, 月花 正幸, 栗栖 宏多, 新保 大輔, 長内 俊也, 中山 若樹, 数又 研, 宝金 清博 脳循環代謝 29 (1) 96 -96 2017年11月 [査読無し][通常論文]
  • 月花正幸, 鐙谷武雄, 小松晃之, 船木亮佑, 栗栖宏太, 新保大輔, 中山若樹, 数又研, 寳金清博 脳循環代謝(Web) 29 (1) 217 -217 2017年11月 [査読無し][通常論文]
  • 機能性蛋白質製剤の最前線 脳梗塞治療薬としてのヘモアクトの効果
    鐙谷 武雄, 月花 正幸, 小松 晃之, 船木 亮佑, 川堀 真人, 長内 俊也, 中山 若樹, 数又 研, 寳金 清博 人工血液 25 (1) 26 -26 2017年11月 [査読無し][通常論文]
  • 月花正幸, 鐙谷武雄, 小松晃之, 船木亮佑, 栗栖宏太, 新保大輔, 中山若樹, 数又研, 寳金清博 脳循環代謝(Web) 29 (1) 217 -217 2017年11月 [査読無し][通常論文]
  • 七戸秀夫, 七戸秀夫, 譚成博, 譚成博, 趙松吉, 趙松吉, 東川桂, 王子豊, 川堀真人, 鐙谷武雄, 右近直之, 安井博宣, 玉木長良, 久下裕司, 寳金清博 脳循環代謝(Web) 29 (1) 155 -155 2017年11月 [査読無し][通常論文]
  • 木村修平, 信田大喜子, 辻岡孝郎, 内田雅也, 上野倫彦, 白石秀明, 笹森徹, 寳金清博, 江夏怜, 越智さと子, 三國信啓, 佐野仁美 日本小児科学会雑誌 121 (11) 1890 -1890 2017年11月01日 [査読無し][通常論文]
  • 難治性てんかんに対して迷走神経刺激療法を導入した3例
    木村 修平, 信田 大喜子, 辻岡 孝郎, 内田 雅也, 上野 倫彦, 白石 秀明, 笹森 徹, 寳金 清博, 江夏 怜, 越智 さと子, 三國 信啓, 佐野 仁美 日本小児科学会雑誌 121 (11) 1890 -1890 2017年11月 [査読無し][通常論文]
  • 機能性蛋白質製剤の最前線 脳梗塞治療薬としてのヘモアクトの効果
    鐙谷 武雄, 月花 正幸, 小松 晃之, 船木 亮佑, 川堀 真人, 長内 俊也, 中山 若樹, 数又 研, 寳金 清博 人工血液 25 (1) 26 -26 2017年11月 [査読無し][通常論文]
  • ラット一過性脳虚血モデルに対する細胞治療 [18F]DPA-714 PETイメージングを用いた神経免疫反応の評価
    七戸 秀夫, 譚 成博, 趙 松吉, 東川 桂, 王 子豊, 川堀 真人, 鐙谷 武雄, 右近 直之, 安井 博宣, 玉木 長良, 久下 裕司, 寳金 清博 脳循環代謝 29 (1) 155 -155 2017年11月 [査読無し][通常論文]
  • ヘモグロビン-アルブミン複合体による脳虚血再灌流急性期治療
    月花 正幸, 鐙谷 武雄, 小松 晃之, 船木 亮佑, 栗栖 宏太, 新保 大輔, 中山 若樹, 数又 研, 寳金 清博 脳循環代謝 29 (1) 217 -217 2017年11月 [査読無し][通常論文]
  • 東海林 菊太郎, 長内 俊也, 川堀 真人, 新谷 好正, 寳金 清博 脳血管内治療 2 (Suppl.) S89 -S89 2017年11月 [査読無し][通常論文]
  • 白井慎一, 松島理明, 加納崇裕, 山崎和義, 濱内祝嗣, 笹森徹, 関俊隆, 矢部一郎, 北川まゆみ, 大槻美佳, 寳金清博, 佐々木秀直 パーキンソン病・運動障害疾患コングレスプログラム・抄録集 11th 91 -91 2017年10月 [査読無し][通常論文]
  • 脳深部刺激療法術前後の体重変化に関する検討
    白井 慎一, 松島 理明, 加納 崇裕, 山崎 和義, 濱内 祝嗣, 笹森 徹, 関 俊隆, 矢部 一郎, 北川 まゆみ, 大槻 美佳, 寳金 清博, 佐々木 秀直 パーキンソン病・運動障害疾患コングレスプログラム・抄録集 11回 91 -91 2017年10月 [査読無し][通常論文]
  • 月花正幸, 寳金清博 日本医師会雑誌 146 S14‐S16 2017年06月15日 [査読無し][通常論文]
  • 脳深部刺激療法後術後12ヵ月まで追跡したパーキンソン病11例のまとめ
    白井 慎一, 松島 理明, 加納 崇裕, 矢部 一郎, 北川 まゆみ, 佐々木 秀直, 濱内 祝嗣, 笹森 徹, 関 俊隆, 宝金 清博, 大槻 美佳 臨床神経学 57 (6) 322 -322 2017年06月 [査読無し][通常論文]
  • 月花 正幸, 寳金 清博 日本医師会雑誌 146 (特別1) S14 -S16 2017年06月 [査読無し][通常論文]
  • 数又研, 寳金清博 Neurological Surgery 45 (5) 443‐449 2017年05月10日 [査読無し][通常論文]
  • 脳神経外科をとりまく医療・社会環境 脳神経外科診療と難病行政
    数又 研, 寳金 清博 Neurological Surgery 45 (5) 443 -449 2017年05月 [査読無し][通常論文]
  • 沢登健治, 佐久嶋研, 荒戸照世, 七戸秀夫, 七戸秀夫, 佐藤典宏, 寳金清博, 寳金清博 レギュラトリーサイエンス学会誌 7 (2) 91‐97(J‐STAGE) -97 2017年05月 [査読無し][通常論文]
     
    北海道大学大学院医学研究科は、厚生労働省のシーズ開発・ガイドライン作成・人材交流を目的とした研究事業に採択され、事業実施機関のうちのひとつとして脳梗塞の再生医療に関するプロジェクトを行っている。本プロジェクトによりアカデミアに蓄積される知識や経験を、個々の人材や組織に蓄積されるに留めてしまうのではなく、広く多くのアカデミアが入手・活用できる形とすることが重要である。そのため、規制当局とアカデミアの両方の視点からプロジェクトを振り返り、アカデミアにおける開発の要点をまとめた。振り返りの方法として、これまでのプロジェクトの進め方および定例会議の内容を整理したうえでグループディスカッションを行った。抽出された主な要点として、プロジェクトの進行を遅らせた要因では薬事リテラシーの不足と開発チームの役割分担、よかった点ではプログラムオフィサーによるレビューと規制当局との対面助言が挙げられた。改善策として専門性に基づく役割分担が考えられ、今後の提言として治験が実施されている医療現場の情報の活用が示された。今後、アカデミアが適切かつ迅速に開発を進めるために、今回議論された点を考慮して開発を進めることが重要と考える。(著者抄録)
  • 関 俊隆, 飛騨 一利, 宝金 清博 小児の脳神経 42 (2) 139 -139 2017年04月 [査読無し][通常論文]
  • 鐙谷武雄, 寳金清博, 小松晃之 先進医薬研究振興財団研究成果報告集 2016 166‐167 2017年03月25日 [査読無し][通常論文]
  • 人工酸素運搬体HemoActを用いた新規脳梗塞治療法の開発
    鐙谷 武雄, 寳金 清博, 小松 晃之 先進医薬研究振興財団研究成果報告集 2016年度 166 -167 2017年03月 [査読無し][通常論文]
  • 川堀真人, 七戸秀夫, 譚成博, 王子ほう, 鐙谷武雄, 中山若樹, 数又研, 宝金清博 再生医療 16 273 2017年02月01日 [査読無し][通常論文]
  • 高宮宗一朗, 高宮宗一朗, 笹森徹, 笹森徹, 濱内祝嗣, 濱内祝嗣, 関俊隆, 関俊隆, 加納崇裕, 矢部一郎, 佐々木秀直, 寳金清博, 寳金清博 Neurological Surgery 45 (1) 53‐58 -58 2017年01月10日 [査読無し][通常論文]
     
    ITB(inrathecal baclofen)療法の方法は通常、薬液を充填したプログラム式輸液ポンプを腹部皮下もしくは腹直筋膜下へ留置し、脊髄クモ膜下腔へ留置した脊髄側カテーテルとコネクターおよびポンプ側カテーテルで接続することにより、脊髄周囲への持続的な薬剤投与を可能とする。今回、ITBポンプ植込み術の際に脊髄側カテーテル断端からの髄液流出は良好であったものの、実際には脊髄側カテーテル先端部が硬膜下腔へ留置されてしまっていた症例を2例経験した。症例は30代女性と20代男性で、いずれも術中カテーテル造影を行うことで脊髄側カテーテルを確実にクモ膜下腔へ留置することができた。具体的には、脊髄側カテーテルのスタイレットを抜去後に、カテーテル断端からイソビスト注240をX線透視下で圧入するだけの簡便な操作を加えることで、脊髄側カテーテルが確実にクモ膜下腔へ挿入されたことを確認することが可能であった。
  • 白井慎一, 松島理明, 加納崇裕, 矢部一郎, 北川まゆみ, 佐々木秀直, 濱内祝嗣, 笹森徹, 関俊隆, 宝金清博, 大槻美佳 臨床神経学(Web) 57 (6) 2017年
  • 伊師雪友, 山口秀, 吉田道春, 茂木洋晃, 小林浩之, 寺坂俊介, 寶金清博 日本分子脳神経外科学会プログラム・抄録集 18th 2017年
  • 茂木洋晃, 山口秀, 寺坂俊介, 小林浩之, 遠藤将吾, 金子貞洋, 伊師雪友, 吉田道春, 宝金清博 日本脳腫瘍の外科学会プログラム・抄録集 22nd 2017年
  • 高宮宗一朗, 茂木洋晃, 伊師雪友, 山口秀, 小林浩之, 寺坂俊介, 寶金清博 日本脳腫瘍の外科学会プログラム・抄録集 22nd 2017年
  • Kiyohiro Houkin, Hideo Shichinohe Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials v -vii 2017年01月01日 [査読無し][通常論文]
  • Kiyohiro Houkin, Koji Abe, Satoshi Kuroda Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials 1 -156 2017年01月01日 [査読無し][通常論文]
     
    This book presents comprehensive reviews for both translational research and clinical trials on cell therapy for stroke. Cerebral stroke is still a leading cause of death and disability. However, despite intensive research, few treatment options are available. The therapeutic potential of cell transplantation has been studied for various pathological conditions of the central nervous system (CNS) including traumatic brain injury, traumatic spinal cord injury, degenerative disease, demyelinating disease and ischemic stroke, as the injured neural tissue in the CNS has only a limited regenerative capacity. Recently, a growing body of evidence in this field suggests that cell transplantation holds great potential as a form of stroke therapy. The authors, who are experts in the field of neurosurgery, review and discuss optimal cell sources and various issues involved in translational research further, they outline ongoing clinical trials in Japan.
  • 鐙谷武雄, 月花正幸, 栗栖宏多, 新保大輔, 長内俊也, 中山若樹, 数又研, 宝金清博 脳循環代謝(Web) 29 (1) 96 2017年 [査読無し][通常論文]
  • 七戸秀夫, 川堀真人, 寳金清博 日本ニューロリハビリテーション学会学術集会プログラム・抄録集 8th 44 2017年 [査読無し][通常論文]
  • 伊師雪友, 山口秀, 吉田道春, 茂木洋晃, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍学会プログラム・抄録集 35th 102 2017年 [査読無し][通常論文]
  • 山口秀, 平田健司, 伊師雪友, 茂木洋晃, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍学会プログラム・抄録集 35th 158 2017年 [査読無し][通常論文]
  • 舘澤諒大, 舘澤諒大, 長内俊也, 関俊隆, 笹森徹, 牛越聡, 飛騨一利, 淺野剛, 井須豊彦, 寳金清博 日本脊髄外科学会プログラム・抄録集 32nd 63 2017年 [査読無し][通常論文]
  • 中山若樹, 月花正幸, 伊藤康裕, 東海林菊太郎, 川堀真人, 長内俊也, 数又研, 鐙谷武雄, 寳金清博 日本臨床脳神経外科学会プログラム・抄録集 20th 116 2017年 [査読無し][通常論文]
  • 笹森徹, 矢野俊介, 飛騨一利, 浜内祝嗣, 関俊隆, 寳金清博, 齋藤久壽 日本脊髄外科学会プログラム・抄録集 32nd 120 2017年 [査読無し][通常論文]
  • 寳金清博 もやもや病(ウイリス動脈輪閉塞症)の診断・治療に関する研究 平成26-28年度 総合研究報告書(Web) WEB ONLY 2017年 [査読無し][通常論文]
  • 寳金清博 もやもや病(ウイリス動脈輪閉塞症)の診断・治療に関する研究 平成28年度 総括・分担研究報告書(Web) 201610037A0003‐201610037A0004 (WEB ONLY) 2017年 [査読無し][通常論文]
  • 伊師雪友, 山口秀, 茂木洋晃, 吉田道春, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 22nd 148 2017年 [査読無し][通常論文]
  • 新谷好正, 大浦大輔, 後藤秀補, 川堀真人, 岩崎素之, 井原達夫, 馬渕正二, 寳金清博 Neurosurgical Emergency 21 (3) 321 2017年 [査読無し][通常論文]
  • 山口秀, 寺坂俊介, 伊師雪友, 茂木洋晃, 小林浩之, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 22nd 122 2017年 [査読無し][通常論文]
  • 笹森徹, 矢野俊介, 濱内祝嗣, 関俊隆, 飛騨一利, 寳金清博 機能的脳神経外科 55 22‐26 -26 2016年12月26日 [査読無し][通常論文]
     
    【はじめに】脊髄刺激療法(SCS)のトライアル期間中、しばしばlead migration(LM)によるparesthesiaの性状や範囲の変化を経験する。しかし、経時的かつ定量的にSCSのLMを検討した報告は少ない。【目的】SCSトライアル期間における、頭尾側方向のLMを定量的に評価すること。LMの程度とリード挿入高位、脊柱管内リード長との関連の有無を評価すること。【対象・方法】難治性疼痛に対してSCSトライアルを行った14例、24本の8極経皮リードを評価の対象とした。脊椎X線側面像を用いて、術直後の画像を基準に、術翌日、トライアル終了時の2点で頭尾側方向へのLMを計測した。また、リード挿入高位と脊柱管内リードの椎体カバー数を調べ、LMとの関連を検討した。【結果】術翌日では、全てのリードは尾側へ平均16.7±10.9mm(4.7〜49.1)移動していた。また、術後平均6.4日(4〜9)のトライアル終了時では、24本中22本(91.7%)のリードにおいて、平均19.0±13.1mm(2.2〜53.2)の尾側移動が認められた。LMとリード挿入高位、脊柱管内リードの椎体カバー数との間に、有意な関連は認められなかった。【結語】SCSトライアル期間中、全てのリードにおいて、尾側へのLMが確認された。トライアル終了時には、90%以上のリードにおいて、尾側方向へ平均19mmのLMが確認された。リードの留置部位を決定する際には、尾側へのLMの可能性を考慮し、疼痛部へparesthesiaを誘発可能な脊椎高位へ、中央より尾側の電極を配置するよう心がけることが重要と思われる。(著者抄録)
  • 関俊隆, 飛騨一利, 矢野俊介, 笹森徹, 浜内祝嗣, 岩崎喜信, 寳金清博 脊髄外科 30 (3) 296‐298 -298 2016年12月25日 [査読無し][通常論文]
     
    脊髄係留症候群(TCS)に対して手術を行った31例(男児16例、女児15例)を対象に、その治療成績を後方的に解析して、TCSの臨床像および無症候性TCSの手術時期について検討した。その結果、症候性TCSは19例、無症候性TCSは12例であった。手術時月齢中央値は症候性TCSは58ヵ月、無症候性TCSは32ヵ月で有意差を認めた。泌尿器科検査にて、術前は18例に、最終経過観察時も16例に膀胱直腸障害を認めた。また、運動機能障害を術前、最終経過観察時とも7例に認め、全例症候性TCSであった。感覚障害は術前6例、最終経過観察時7例に認められた。単変量解析では35ヵ月未満で手術を行った症例で、予後良好である傾向があったが、多変量解析では有意な予後因子は認めなかった。
  • 七戸秀夫, 寳金清博 日本神経学会学術大会プログラム・抄録集 57th (Suppl.) 176 -S47 2016年12月 [査読無し][通常論文]
  • 内野晴登, 寳金清博 小児内科 48 331‐335 2016年11月30日 [査読無し][通常論文]
  • 内頸動脈(C2)瘤の手術治療成績の検討 直達手術と血管内治療との比較
    下田 祐介, 牛越 聡, 安田 宏, 安喰 稔, 長内 俊也, 中山 若樹, 宝金 清博 脳血管内治療 1 (Suppl.) S124 -S124 2016年11月 [査読無し][通常論文]
  • 硬膜動静脈瘻に対する液状塞栓物質を用いた経動脈的塞栓術
    浅野 剛, 久保田 真彰, 久保田 俊介, 荷堂 謙, 三ツ橋 茂雄, 山上 岩男, 景山 雄介, 櫻井 寿郎, 宝金 清博 脳血管内治療 1 (Suppl.) S131 -S131 2016年11月 [査読無し][通常論文]
  • EZO trialの初期成績
    長内 俊也, 牛越 聡, 青樹 毅, 宝金 清博 脳血管内治療 1 (Suppl.) S269 -S269 2016年11月 [査読無し][通常論文]
  • 急性期脳梗塞における脳血栓回収療法後のASL高信号部位は最終脳梗塞部位を予想しうる
    後藤 秀輔, 川堀 真人, 岩崎 素之, 新谷 好正, 馬渕 正二, 長内 俊也, 宝金 清博 脳血管内治療 1 (Suppl.) S66 -S66 2016年11月 [査読無し][通常論文]
  • 血栓回収療法におけるアプローチルート評価目的の心電図同期大動脈弓MRAの有効性の検討
    川堀 真人, 長内 俊也, 後藤 秀輔, 新谷 好正, 岩崎 素之, 馬渕 正二, 宝金 清博 脳血管内治療 1 (Suppl.) S190 -S190 2016年11月 [査読無し][通常論文]
  • 【小児疾患診療のための病態生理 3 改訂第5版】 神経疾患 もやもや病
    内野 晴登, 寳金 清博 小児内科 48 (増刊) 331 -335 2016年11月 [査読無し][通常論文]
  • 再生医療の基礎と臨床UP to DATE 脳梗塞に対する自家骨髄間質細胞移植
    七戸 秀夫, 寳金 清博 脳循環代謝 28 (1) 124 -124 2016年11月 [査読無し][通常論文]
  • 全身麻酔下頸動脈ステント留置術の有用性と術中モニタリング
    月花 正幸, 青樹 毅, 長内 俊也, 中山 若樹, 数又 研, 鐙谷 武雄, 七戸 秀夫, 寳金 清博 脳血管内治療 1 (Suppl.) S224 -S224 2016年11月 [査読無し][通常論文]
  • 穂刈正昭, 寳金清博 Clinical Neuroscience 34 (11) 1231‐1235 2016年11月01日 [査読無し][通常論文]
  • 月花正幸, 鐙谷武雄, 小松晃之, 船木亮佑, 栗栖宏太, 中山若樹, 数又研, 寶金清博 人工血液 24 (1) 33 2016年10月31日 [査読無し][通常論文]
  • 笹森徹, 関俊隆, 寳金清博, 太田穣, 矢野俊介, 飛騨一利 北海道整形災害外科学会雑誌 58 (1) 164 -164 2016年10月24日 [査読無し][通常論文]
  • 加納 崇裕, 白井 慎一, 矢部 一郎, 濱内 祝嗣, 笹森 徹, 関 俊隆, 寶金 清博, 佐々木 秀直 神経治療学 33 (5) S193 -S193 2016年10月 [査読無し][通常論文]
  • 【細胞移植と神経再生】 細胞移植と神経再生
    七戸 秀夫, 寳金 清博 Clinical Neuroscience 34 (10) 1082 -1085 2016年10月 [査読無し][通常論文]
  • 白井 慎一, 加納 崇裕, 長沼 亮滋, 松島 理明, 矢部 一郎, 笹森 徹, 濱内 祝嗣, 関 俊隆, 寳金 清博, 佐々木 秀直 神経治療学 33 (5) S204 -S204 2016年10月 [査読無し][通常論文]
  • 七戸秀夫, 寳金清博 Clinical Neuroscience 34 (10) 1082‐1085 2016年10月01日 [査読無し][通常論文]
  • 小林 聡, 山口 秀, 遠藤 将吾, 小林 浩之, 寺坂 俊介, 畑中 佳奈子, 宝金 清博 日本小児血液・がん学会雑誌 53 (2) 165 -165 2016年07月 [査読無し][通常論文]
  • 研究 髄膜腫の術前塞栓の有用性 エンボスフィアの使用経験
    下田 祐介, 長内 俊也, 寺坂 俊介, 小林 浩之, 山口 秀, 遠藤 将吾, 畑中 佳奈子, 寳金 清博 Neurological Surgery 44 (7) 555 -560 2016年07月 [査読無し][通常論文]
     
    髄膜腫における術前塞栓の有用性について検討した。巨大な髄膜腫摘出術前にエンボスフィアで塞栓術を実施した4例(男性1名、女性3名、65〜79歳)を対象とした。塞栓術後、平均腫瘍最大径は58.8±9.2→57.3±10.8mm、平均腫瘍体積は70.6±41.5→66.7±36.8mLと減少したが、有意差はなかった。術中の出血量は、塞栓術後6日目に摘出をした症例で最も少なかった。エンボスフィアはHE染色で内部が無構造であった。摘出標本では4例中2例に壊死を認めた。標本中の局在は主として血管内であるため周囲に血管壁構造を伴うことが多く、von Kossa染色で染色されないことが特徴として挙げられた。合併症として網膜中心動脈閉塞症が1例でみられた。
  • 下田祐介, 長内俊也, 寺坂俊介, 小林浩之, 山口秀, 遠藤将吾, 畑中佳奈子, 寳金清博 Neurological Surgery 44 (7) 555 -60 2016年07月 [査読無し][通常論文]
     
    Embosphere® was approved in Japan for embolizing intracranial arteries for the reduction of intraoperative bleeding in January 2014. Until August 2015, we performed embolization for four meningiomas using Embosphere®. We performed an initial evaluation of all the cases, including evaluation of the clinical courses, change in the maximum tumor diameters and volumes, alteration in the appearance on magnetic resonance imaging(MRI), amount of intraoperative bleeding, complications, and histopathological findings. After embolization, the maximum tumor diameters and volumes slightly decreased on MRI, whereas the signal change on diffusion-weighted imaging(DWI)or fluid-attenuated inversion recovery(FLAIR)varied in each case. One case demonstrated a partial signal change on DWI one day after the initial procedure, and another case demonstrated a decrease in perifocal edema on FLAIR. Among our patients, least bleeding was recorded at 6 days after the embolization. Histopathological analysis revealed coagulative necrotic lesions in two cases. The von Kossa stain was used to distinguish Embosphere® from the psammoma body. One case involved a thromboembolic complication of the retinocentral artery caused by an Embosphere® of 100-300 μm. Our early-stage experience suggests that an Embosphere® of 300-500 μm should be used for safe embolization before resection to avoid thromboembolic complications because an Embosphere® of 100-300 μm can pass through the dangerous anastomosis. Our present strategy was to resect the tumor approximately seven days after the embolization using Embosphere®. However, further studies and discussion on the size of Embosphere®, and the interval between pre-operative embolization and surgical removal are needed.
  • 脳深部刺激療法(DBS)の刺激周波数変更によって歩行障害が改善したパーキンソン病(PD)の1例
    上床 尚, 長井 梓, 小渡 貴司, 白井 慎一, 松島 理明, 中野 史人, 廣谷 真, 加納 崇裕, 北川 まゆみ, 大槻 美佳, 笹森 徹, 関 俊隆, 矢部 一郎, 寶金 清博, 佐々木 秀直 臨床神経学 56 (6) 450 -450 2016年06月 [査読無し][通常論文]
  • 両側GPi-DBSにより嚥下機能改善を認めた頸部ジストニアの1例
    笹森 徹, 浜内 祝嗣, 関 俊隆, 松島 理明, 加納 崇裕, 矢部 一郎, 安彦 かがり, 佐々木 秀直, 寳金 清博 臨床神経学 56 (6) 450 -450 2016年06月 [査読無し][通常論文]
  • 浜内 祝嗣, 関 俊隆, 笹森 徹, 宝金 清博 小児の脳神経 41 (1) 87 -87 2016年05月 [査読無し][通常論文]
  • 月花 正幸, 長内 俊也, 数又 研, 牛越 聡, 高宮 宗一郎, 斉藤 巧実, 中山 若樹, 七戸 秀夫, 鐙谷 武雄, 宝金 清博 脳神経外科速報 26 (5) 515 -521 2016年05月 [査読無し][通常論文]
     
    症例は30歳代女性で、6ヵ月前、頭痛を主訴に受診し、類もやもや病と右後大脳動脈(PCA)血栓化動脈瘤と診断され経過観察となった。1ヵ月前より、突発的な右眼周囲痛の激しい痛みが出現し、動脈瘤の明らかな増大を認めた。三叉神経痛様の右眼周囲痛に対して、カルバマゼピンを処方し軽度症状の改善を認めた。急速な動脈瘤の増大と新たな脳神経障害を認めているため手術の方針となった。片側の類もやもや病と右PCAの下向き大型部分血栓化動脈瘤の診断にて、EC-IC bypass併用の血管内母血管閉塞術を試みた。術2週間後にステント併用コイル塞栓術を施行した。術翌日から右眼周囲の疼痛は消失し、カルバマゼピンは休薬した。術後9ヵ月後のMRIでは若干の血栓化瘤の縮小を認め、疼痛の再発は認めない。
  • 山口 秀, 伊師 雪友, 畑中 佳奈子, 西原 広史, 吉田 道春, 遠藤 将吾, 茂木 洋晃, 小林 浩之, 寺坂 俊介, 寳金 清博 Brain Tumor Pathology 33 (Suppl.) 067 -067 2016年05月 [査読無し][通常論文]
  • 伊師 雪友, 山口 秀, 畑中 佳奈子, 藤田 裕美, 吉田 道春, 遠藤 将吾, 小林 浩之, 寺坂 俊介, 寳金 清博 Brain Tumor Pathology 33 (Suppl.) 130 -130 2016年05月 [査読無し][通常論文]
  • 杉山 拓, 中山 若樹, 数又 研, 新保 大輔, 月花 正幸, 内田 和希, 穂刈 正昭, 浅岡 克行, 板本 孝治, 寳金 清博 脳卒中の外科 44 (3) 183 -188 2016年05月 [査読無し][通常論文]
     
    クリッピング困難な内頸動脈瘤に対してバイパス併用手術を行った50例の治療成績について検討した。その結果、1)41例で良好な転帰が得られていたが、術後に出血性合併症を1例、虚血性合併症を8例で認められた。2)合併率を部位別に検討すると、Segment 1で5.2%、Segment 2で16.7%、Segment 3で55.6%と、Segment 3で合併症が多く認められた。一方、単変量解析でみた、術後の合併症に関係する因子としては、性別と動脈瘤の部位が有意にあり、特に動脈瘤の部位で強い相関が示された。だが、多変量解析ではいずれの因子も有意と判定されなかった。
  • 杉山 拓, 中山 若樹, 数又 研, 新保 大輔, 月花 正幸, 内田 和希, 穂刈 正昭, 浅岡 克行, 板本 孝治, 寳金 清博 脳卒中の外科 44 (3) 183 -188 2016年05月 [査読無し][通常論文]
     
    クリッピング困難な内頸動脈瘤に対してバイパス併用手術を行った50例の治療成績について検討した。その結果、1)41例で良好な転帰が得られていたが、術後に出血性合併症を1例、虚血性合併症を8例で認められた。2)合併率を部位別に検討すると、Segment 1で5.2%、Segment 2で16.7%、Segment 3で55.6%と、Segment 3で合併症が多く認められた。一方、単変量解析でみた、術後の合併症に関係する因子としては、性別と動脈瘤の部位が有意にあり、特に動脈瘤の部位で強い相関が示された。だが、多変量解析ではいずれの因子も有意と判定されなかった。
  • 数又研, 内野晴登, 中山若樹, 七戸秀夫, 鐙谷武雄, 寶金清博 脳神経外科ジャーナル 25 (Supplement) 80 2016年04月20日 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博 Medical Practice 33 (3) 417 -419 2016年03月 [査読無し][通常論文]
  • 関 俊隆, 浜内 祝嗣, 笹森 徹, 月花 正幸, 伊藤 康裕, 小林 聡, 斉藤 拓実, 宝金 清博 小児の脳神経 40 (3) 249 -251 2016年03月 [査読無し][通常論文]
  • Yoshimasa Niiya, Masato Kawabori, Motoyuki Iwasaki, Shoji Mabuchi, Kiyohiro Houkin STROKE 47 2016年02月 [査読無し][通常論文]
  • Haruto Uchino, Masaki Ito, Ken Kazumata, Kiyohiro Houkin STROKE 47 2016年02月 [査読無し][通常論文]
  • 沢登健治, 佐久嶋研, 荒戸照世, 七戸秀夫, 佐藤典宏, 寶金清博, 寶金清博 再生医療 15 310 2016年02月01日 [査読無し][通常論文]
  • 譚成博, WANG Zifeng, 濱内祝嗣, 鐙谷武雄, 中山若樹, 数又研, 穂刈正昭, 七戸秀夫, 寶金清博 再生医療 15 291 2016年02月01日 [査読無し][通常論文]
  • Hisayasu Saito, Satoshi Kuroda, Songji Zhao, Naoki Kubo, Yuji Kuge, Nagara Tamaki, Hideo Shichinohe, Kiyohiro Houkin Stroke 47 (suppl 1) TP99 2016年02月 [査読無し][通常論文]
  • 七戸秀夫, 譚成博, 濱内祝嗣, 王子豊, 鐙谷武雄, 中山若樹, 数又研, 寳金清博 再生医療 15 268 2016年02月01日 [査読無し][通常論文]
  • 遠藤将吾, 小林浩之, 寺坂俊介, 山口秀, 浅岡克行, 茂木洋晃, 金子貞洋, 寳金清博 日本間脳下垂体腫よう学会プログラム・抄録集 26th 94 2016年01月29日 [査読無し][通常論文]
  • 笹森徹, 浜内祝嗣, 関俊隆, 松島理明, 加納崇裕, 矢部一郎, 安彦かがり, 佐々木秀直, 寶金清博 臨床神経学(Web) 56 (6) 2016年
  • 山口秀, 寺坂俊介, 茂木洋晃, 伊師雪友, 吉田道春, 小林浩之, 寶金清博 日本脳腫瘍学会プログラム・抄録集 34th 2016年
  • 下田祐介, 牛越聡, 安田宏, 安喰稔, 長内俊也, 中山若樹, 宝金清博 脳血管内治療(Web) 1 (Supplement) S124(J‐STAGE) 2016年 [査読無し][通常論文]
  • 月花正幸, 青樹毅, 長内俊也, 中山若樹, 数又研, 鐙谷武雄, 七戸秀夫, 寳金清博 脳血管内治療(Web) 1 (Supplement) S224(J‐STAGE) 2016年 [査読無し][通常論文]
  • 中山若樹, 長内俊也, 寳金清博 脳神経外科ジャーナル 25 (1) 15‐26 (J‐STAGE) -26 2016年 [査読無し][通常論文]
     
    巨大動脈瘤や解離性動脈瘤などの, クリッピング自体が困難な動脈瘤に対しては, 何らかのバイパス複合手術が行われる. 動脈瘤の諸条件やその治療戦略は実に多岐にわたり, 現代において治療法を組み立てる考え方は, 共通の理解のもとで徐々に確立されつつある. バイパスとしてはSTAやOAなどのlow flowとRAなどによるhigh flowがあり, バイパスの意義としては, ①最終的にクリッピングに持ち込むまでの長時間母血管遮断に対する虚血保護, ②クリッピングに伴い犠牲になる分枝のalteration, ③トラッピングもしくは母血管閉塞に伴うflow reversalに大別される. いずれにおいても, 正常脳灌流が着実に担保されるように, 手術戦略を綿密に計画する必要がある.
     そして実際の手術においては, 計画した手術内容が的確に効果を発揮しているかを判断するための術中モニタリングがきわめて重要である. MEPやSEPなどの電気生理学的モニタリングは必須であろう. また選択するバイパスの種別が適切な流量かの判断は時として難しい場合があるが, 手術台と血管造影装置によるハイブリッド手術室の利用や, ICG蛍光血管造影/FLOW800®, transit-time血流計などが的確な術中判断に寄与してくれる.
  • 齊藤正樹, 板本孝治, 鐙谷武雄, 大西浩文, 浅川直也, 榊原守, 橋本暁佳, 成澤弘美, 下濱俊, 三國信啓, 三浦哲嗣, 寳金清博, 村松公美子, 村松公美子, 伊藤弘人 日本循環器心身医学会抄録集 73rd 44 2016年 [査読無し][通常論文]
  • 笹森徹, 矢野俊介, 濱内祝嗣, 関俊隆, 飛騨一利, 寳金清博 日本脊髄外科学会プログラム・抄録集 31st 180 2016年 [査読無し][通常論文]
  • 七戸秀夫, 寳金清博 日本ニューロリハビリテーション学会学術集会プログラム・抄録集 7th 43 2016年 [査読無し][通常論文]
  • 小林浩之, 寺坂俊介, 永井正一, 山口秀, 茂木洋晃, 遠藤将吾, 黒田敏, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 21st 133 2016年 [査読無し][通常論文]
  • 茂木洋晃, 寺坂俊介, 小林浩之, 山口秀, 遠藤将吾, 金子貞洋, 伊師雪友, 吉田道春, 能條建, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 21st 129 2016年 [査読無し][通常論文]
  • 伊師雪友, 山口秀, 畑中佳奈子, 藤田裕美, 吉田道春, 茂木洋晃, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍学会プログラム・抄録集 34th 91 2016年 [査読無し][通常論文]
  • 吉田道春, 山口秀, 伊師雪友, 遠藤将吾, 金子貞洋, 茂木洋晃, 小林浩之, 浅岡克行, 鴨嶋雄大, 寺坂俊介, 寳金清博 日本脳腫瘍学会プログラム・抄録集 34th 116 2016年 [査読無し][通常論文]
  • 高宮宗一朗, 長内俊也, 牛越聡, 栗栖宏多, 下田祐介, 伊藤康裕, 伊師雪友, 穂刈正昭, 中山若樹, 数又研, 鐙谷武雄, 七戸秀夫, 寳金清博 Neurological Surgery 44 (1) 39 -45 2016年01月 [査読無し][通常論文]
     
    Systemic vascular diseases such as fibromuscular dysplasia, Ehlers-Danlos syndrome, Marfan syndrome, and Behçet's disease are known to cause spontaneous dissecting aneurysms of the cervical internal carotid artery. These diseases are generally associated with vascular fragility; therefore, invasive treatments are avoided in many cases of dissecting aneurysms, and a conservative approach is used for the primary disease. Surgical or intravascular treatment may be chosen when aneurysms are progressive or are associated with a high risk of hemorrhage; however, there is no consensus on which treatment is better. We report a case of a dissecting aneurysm of the cervical internal carotid artery in a patient with suspected Behçet's disease, which was treated using stent-assisted coil embolization. A man in his 40's, with suspected Behçet's disease, presented with an enlarged dissecting aneurysm of the right cervical internal carotid artery. The lesion was present for approximately 10 years. We performed stent-assisted coil embolization for the lesion. Post-surgery, no aneurysms were detected with carotid artery echography. Our case report suggests that stent-assisted coil embolization is a promising treatment for dissecting aneurysms of the cervical internal carotid artery. In addition, the procedure demonstrates the utility of carotid artery echograms for examining recanalization after stent-assisted coil embolization.
  • 関俊隆, 飛彈一利, 岩崎喜信, 小柳泉, 矢野俊介, 青山剛, 笹森徹, 浜内祝嗣, 寳金清博 脊髄外科 29 (3) 326‐329 2015年12月25日 [査読無し][通常論文]
  • 脳深部刺激療法前後での心理学的評価項目の変化について
    加納 崇裕, 松島 理明, 矢部 一郎, 北川 まゆみ, 佐々木 秀直, 大槻 美佳, 濱内 祝嗣, 笹森 徹, 関 俊隆, 寶金 清博, 井上 猛 臨床神経学 55 (12) 945 -945 2015年12月 [査読無し][通常論文]
  • 月花正幸, 長内俊也, 数又研, 牛越聡, 高宮宗一郎, 斉藤巧実, 中山若樹, 七戸秀夫, 鐙谷武雄, 寶金清博 Journal of Neuroendovascular Therapy 9 (6) S374 2015年11月02日 [査読無し][通常論文]
  • 内野 晴登, 伊東 雅基, 宝金 清博 脳神経外科速報 25 (11) 1185 -1192 2015年11月 [査読無し][通常論文]
     
    もやもや病は、内頸動脈終末部の進行性狭窄と脳底部を中心とする異常血管網の発達を特徴とする原因不明の希少疾患である。2011年にわが国で感受性遺伝子RNF213が同定され、その臨床的意義や生理的機能の解明に向けた研究が続けられている。ただし、RNF213変異単独では、本疾患の発症に関してすべてを説明することはできていない。そのため、その他の因子の関与が強く想定され、エピジェネティクスの観点からも病態研究が始められつつある。本稿では、もやもや病の疫学的、病理学的背景のほか、もやもや病遺伝研究の歴史的変遷と最新の知見について述べる。(著者抄録)
  • 頸動脈分岐部に生じた可動性プラークに対するCASとCEAの治療戦略
    川堀 真人, 長内 俊也, 新谷 好正, 岩崎 素之, 馬渕 正二, 寶金 清博 JNET: Journal of Neuroendovascular Therapy 9 (6) S403 -S403 2015年11月 [査読無し][通常論文]
  • 笹森徹, 飛騨一利, 矢野俊介, 関俊隆, 寳金清博 脊椎脊髄ジャーナル 28 (10) 871 -874 2015年10月25日 [査読無し][通常論文]
  • もやもや病周術期におけるASL-4DMRAを用いた脳循環評価
    内野 晴登, 伊東 雅基, 数又 研, 藤間 憲幸, 中山 若樹, 宝金 清博 脳循環代謝 27 (1) 131 -131 2015年10月 [査読無し][通常論文]
  • 外傷後急性硬膜下血腫により明らかとなった急性リンパ性白血病の1例
    高宮 宗一朗, 早川 峰司, 水柿 明日美, 方波見 謙一, 宮本 大輔, 寶金 清博, 丸藤 哲 日本救急医学会雑誌 26 (10) 625 -631 2015年10月 [査読無し][通常論文]
     
    頭部外傷による急性硬膜下血腫の治療中に明らかになった急性リンパ性白血病の1例を報告する。患者は6歳の男児。頭部打撲後に嘔吐、意識障害を伴って当科に救急搬送された。搬入時、深昏睡、瞳孔不同などから頭蓋内出血を疑い、緊急気管挿管後に頭部CTを施行した。急性硬膜下血腫の診断で緊急穿頭血腫除去術および開頭血腫除去術を行った。術後経過は良好であったが、汎血球減少を認めたため骨髄生検を施行した。急性リンパ性白血病の診断となり、頭蓋形成術後に化学療法を開始し、寛解を得た。重症頭部外傷では、出血や輸血、炎症などにより採血データに修飾が加わり、受傷直後の白血病の鑑別は困難であるが、経時的な観察により感知可能である。また、頭蓋内出血を伴う白血病においては、その治療にあたり、中枢神経浸潤を考慮する必要がある。(著者抄録)
  • 新保 大輔, 鐙谷 武雄, 栗栖 宏多, 七戸 秀雄, 中山 若樹, 数又 研, 宝金 清博 脳循環代謝 27 (1) 127 -127 2015年10月 [査読無し][通常論文]
  • 捻転ジストニアに対するバクロフェン髄注療法投与方法の検討
    長沼 亮滋, 佐藤 智香, 高橋 育子, 松島 理明, 中野 史人, 佐藤 和則, 廣谷 真, 加納 崇裕, 矢部 一郎, 佐々木 秀直, 笹森 徹, 関 俊隆, 寶金 清博, 松尾 雄一郎, 生駒 一憲 臨床神経学 55 (9) 681 -681 2015年09月 [査読無し][通常論文]
  • 関俊隆, 飛騨一利, 矢野俊介, 青山剛, 岩崎喜信, 寳金清博 脊髄外科 29 (2) 191 -193 2015年08月25日 [査読無し][通常論文]
  • 沢登健治, 佐久嶋研, 荒戸照世, 七戸秀夫, 佐藤典宏, 寶金清博, 寶金清博 レギュラトリーサイエンス学会誌 5 (Supplement) 73 2015年08月 [査読無し][通常論文]
  • 齊藤正樹, 齊藤正樹, 山本和利, 山本和利, 米増保之, 小松克也, 三上毅, 外山祐一郎, 鈴木秀一郎, 松村晃寛, 寺本篤史, 寺本篤史, 射場浩介, 三國信啓, 寳金清博, 有山静香, 橋本諭, 下濱俊 医学教育 46 (Suppl.) 177 -177 2015年07月10日 [査読無し][通常論文]
  • 吉田道春, 山口秀, 伊師雪友, 遠藤将吾, 茂木洋晃, 小林浩之, 浅岡克行, 鴨嶋雄大, 寺坂俊介, 寳金清博 Neurol Surg 43 (7) 603 -610 2015年07月10日 [査読無し][通常論文]
     
    摘出術施行後の摘出腔に徐放性製剤ギリアデルを留置した悪性神経膠腫21例(男13例、女8例、平均58.8歳)における有害事象について報告した。組織型はgrade IIIが8例、IVが13例で、摘出度は完全摘出14例、95%以上摘出4例、95%未満3例、留置時期は初発初回手術8例(A群)、初発時追加手術6例(B群)、再発時手術7例(C群)であった。術後6〜22ヵ月で、有害事象は14例に認め、脳浮腫12例、嚢胞形成4例、痙攣3例などで、CTCAE grade 4以上はなく、grade 3が4例であった。有害事象と組織型、腫瘍摘出度との関連は認めなかったが、留置時期では発生率がA群37.5%、B群83.3%、C群85.7%と差があり、特に脳浮腫はそれぞれ25.0%、66.6%、85.7%とC群がA群に比較して有意に高かった。またCTCAE grade 3はA群0例、B群2例(それぞれ発熱・髄液漏、Gerstmann症候群の脳浮腫)、C群2例(痙攣を伴う脳浮腫)と差異を認めた。
  • 七戸秀夫, 寳金清博 医学のあゆみ 254 (1) 119 -122 2015年07月04日 [査読無し][通常論文]
  • 七戸 秀夫, 寳金 清博 医学のあゆみ 254 (1) 119 -122 2015年07月04日 [査読無し][通常論文]
  • 高橋 淳, 長谷川 泰弘, 峰松 一夫, 木内 博之, 寳金 清博, 西山 和利, 佐賀 恒夫, 下瀬川 恵久, 安斉 俊久, 小笠原 邦昭, 木村 弘, 巽 浩一郎, 陳 和夫, 中川原 譲二, 畑澤 順, 宮本 享, 日本脳卒中学会, 日本脳神経外科学会, 日本神経学会, 日本核医学会, 学会合同アセタゾラミド適正使用指針作成委員会 脳卒中 37 (4) 281 -297 2015年07月 [査読無し][通常論文]
  • Toru Sasamori, Shunsuke Yano, Kazutoshi Hida, Yasuhiro Chiba, Daijiro Morimoto, Toshitaka Seki, Toyohiko Isu, Hisatoshi Saito, Kiyohiro Houkin JOURNAL OF NEUROSURGERY 122 (6) A1570 -A1571 2015年06月 [査読無し][通常論文]
  • 寳金 清博 学術の動向 : SCJフォーラム = Trends in the sciences : SCJ Forum 20 (6) 59 -62 2015年06月 [査読無し][通常論文]
  • 吉田道春, 山口秀, 池田拓磨, 小林浩之, 寺坂俊介, 寳金清博, 畑中佳奈子, 松野吉宏, 西原広史 北海道医学雑誌 90 (1) 78 -79 2015年05月01日 [査読無し][通常論文]
  • 関 俊隆, 浜内 祝嗣, 笹森 徹, 月花 正幸, 伊藤 康裕, 小林 聡, 斉藤 拓実, 宝金 清博 小児の脳神経 40 (1) 86 -86 2015年04月 [査読無し][通常論文]
  • Y. Niiya, H. Itosaka, M. Kawabori, M. Iwasaki, S. Mabuchi, K. Houkin INTERNATIONAL JOURNAL OF STROKE 10 384 -384 2015年04月 [査読無し][通常論文]
  • Satoshi Kuroda, AMORE Study Group, Clinical Coordinating Center, Data Management Center, Norihiro Sato, Kuniaki Ogasawara, Koji Iihara, Ken-Ichiro Kikuta, Kiyohiro Houkin, Toshiaki Osato, Teiji Tominaga, Nobuhito Saito, Yoshikazu Okada, Shigeru Nogawa, Tadashi Nariai, Jun-Ichi Ono, Kimitoshi Sato, Kazuo Yamada, Susumu Miyamoto, Susumu Miyamoto, Jun Takahashi, Jyoji Nakagawara, Manabu Sakaguchi, Isao Date, Isao Date, Izumi Nagata Neurologia Medico-Chirurgica 55 (3) 194 -198 2015年02月20日 [査読無し][通常論文]
     
    Recent development of a non-invasive magnetic resonance examination has increased the opportunity to identify asymptomatic patients with moyamoya disease who have experienced no cerebrovascular events. However, their clinical features, prognosis, and treatment strategy are still unclear because of small number of subjects and short follow-up periods. Therefore, we have designed Asymptomatic Moyamoya Registry (AMORE. study in Japan. The objectives of this nation-wide, multi-center prospective study are to clarify long-term prognosis of asymptomatic patients with moyamoya disease and to determine the risk factors that cause ischemic and hemorrhagic stroke in them. In this article, we review the published data on asymptomatic moyamoya disease and report the on-going multi-center prospective cohort study, AMORE study. We would like to emphasize the importance to determine the clinical features, prognosis, and treatment strategies of asymptomatic moyamoya disease in very near future.
  • 吉田道春, 小林浩之, 遠藤将吾, 山口秀, 寺坂俊介, 寳金清博 日本間脳下垂体腫よう学会プログラム・抄録集 25th 73 2015年02月12日 [査読無し][通常論文]
  • Kikutaro Tokairin, Masaki Ito, Ken Kazumata, Sadao Kaneko, Kiyohiro Houkin STROKE 46 2015年02月 [査読無し][通常論文]
  • チーム医療としての脳深部刺激療法 今後の課題
    加納 崇裕, 佐藤 智香, 長沼 亮滋, 高橋 育子, 松島 理明, 中野 史人, 佐藤 和則, 廣谷 真, 矢部 一郎, 北川 まゆみ, 佐々木 秀直, 井上 猛, 笹森 徹, 関 俊隆, 寶金 清博 臨床神経学 55 (2) 130 -130 2015年02月 [査読無し][通常論文]
  • 浜内祝嗣, 七戸秀夫, 伊東雅基, 長内俊也, 穂刈正昭, 中山若樹, 数又研, 江良択実, 寶金清博 再生医療 14 214 2015年02月01日 [査読無し][通常論文]
  • 譚成博, 七戸秀夫, 濱内祝嗣, 鐙谷武雄, 中山若樹, 数又研, 穂刈正昭, 寶金清博 再生医療 14 360 2015年02月01日 [査読無し][通常論文]
  • 七戸秀夫, 譚成博, 濱内祝嗣, 鐙谷武雄, 中山若樹, 数又研, 穂刈正昭, 寳金清博 再生医療 14 302 2015年02月01日 [査読無し][通常論文]
  • 長沼亮滋, 佐藤智香, 高橋育子, 松島理明, 中野史人, 佐藤和則, 廣谷真, 加納崇裕, 矢部一郎, 佐々木秀直, 笹森徹, 関俊隆, 寶金清博, 松尾雄一郎, 生駒一憲 臨床神経学(Web) 55 (9) 2015年
  • 加納崇裕, 松島理明, 矢部一郎, 北川まゆみ, 佐々木秀直, 大槻美佳, 濱内祝嗣, 笹森徹, 関俊隆, 寶金清博, 井上猛 臨床神経学(Web) 55 (12) 2015年
  • 山口秀, 寺坂俊介, 小林浩之, 茂木洋晃, 遠藤将吾, 伊師雪友, 吉田道春, 寶金清博 日本脳腫瘍の外科学会プログラム・抄録集 20th 2015年
  • 茂木洋晃, 寺坂俊介, 小林浩之, 山口秀, 金子貞洋, 遠藤将吾, 伊師雪友, 吉田道春, 能條建, 寶金清博 日本脳腫瘍の外科学会プログラム・抄録集 20th 2015年
  • 山口秀, 伊師雪友, 吉田道春, 遠藤将吾, 小林浩之, 寺坂俊介, 寶金清博 日本脳腫瘍学会プログラム・抄録集 33rd 2015年
  • 中山若樹, 長内俊也, 数又研, 宝金清博 日本脳ドック学会総会プログラム・抄録集 24th 043 2015年 [査読無し][通常論文]
  • 笹森徹, 矢野俊介, 濱内祝嗣, 関俊隆, 飛騨一利, 寳金清博 日本脊髄外科学会プログラム・抄録集 30th 229 2015年 [査読無し][通常論文]
  • 穂刈正昭, 穂刈正昭, 中山若樹, 下田祐介, 鐙谷武雄, 七戸秀夫, 寳金清博 日本脳循環代謝学会総会プログラム・抄録号 27th 141 2015年 [査読無し][通常論文]
  • 加納崇裕, 佐藤智香, 長沼亮滋, 高橋育子, 松島理明, 中野史人, 佐藤和則, 廣谷真, 矢部一郎, 北川まゆみ, 井上猛, 志賀哲, 笹森徹, 関俊隆, 寳金清博, 佐々木秀直 日本神経学会学術大会プログラム・抄録集 56th 441 2015年 [査読無し][通常論文]
  • 遠藤将吾, 工藤信樹, 山口秀, 住吉洸城, 茂木洋晃, 金子貞洋, 小林浩之, 寺坂俊介, 寳金清博 日本超音波医学会光超音波画像研究会プログラム・抄録集 2015 (2nd) 25 -26 2015年 [査読無し][通常論文]
  • 七戸秀夫, 黒田敏, 寳金清博 日本脳循環代謝学会総会プログラム・抄録号 27th 95 2015年 [査読無し][通常論文]
  • 吉田道春, 山口秀, 伊師雪友, 遠藤将吾, 茂木洋晃, 小林浩之, 浅岡克行, 鴨嶋雄大, 寺坂俊介, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 20th 128 2015年 [査読無し][通常論文]
  • 伊師雪友, 山口秀, 吉田道春, 遠藤将吾, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍学会プログラム・抄録集 33rd 177 2015年 [査読無し][通常論文]
  • 笹森徹, 関俊隆, 飛彈一利, 長内俊也, 矢野俊介, 寳金清博 脊髄外科 28 (3) 306 -309 2014年12月25日 [査読無し][通常論文]
  • 笹森 徹, 関 俊隆, 宝金 清博, 加納 崇裕, 矢部 一郎, 佐々木 秀直 北海道整形災害外科学会雑誌 56 (1) 151 -151 2014年12月 [査読無し][通常論文]
  • 小林 聡, 宮本 倫行, 長内 俊也, 下田 祐介, 牛越 聡, 大岡 智学, 松居 善郎, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 8 (6) 374 -374 2014年12月 [査読無し][通常論文]
  • 下田祐介, 長内俊也, 中山若樹, 牛越聡, 内野晴登, 栗栖宏多, 穂刈正昭, 七戸秀夫, 鐙谷武雄, 数又研, 寶金清博 J Neuroendovascular Ther 8 (6) 369 2014年12月01日 [査読無し][通常論文]
  • 長内 俊也, 中山 若樹, 牛越 聡, 下田 祐介, 栗栖 宏多, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 8 (6) 406 -406 2014年12月 [査読無し][通常論文]
  • 栗栖 宏多, 長内 俊也, 下田 祐介, 鐙谷 武雄, 数又 研, 中山 若樹, 七戸 秀夫, 穂刈 正昭, 牛越 聡, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 8 (6) 203 -203 2014年12月 [査読無し][通常論文]
  • 高宮宗一朗, 長内俊也, 牛越聡, 栗栖宏多, 下田祐介, 伊藤康裕, 伊師雪友, 中山若樹, 数又研, 鐙谷武雄, 七戸秀夫, 寶金清博 J Neuroendovascular Ther 8 (6) 434 2014年12月01日 [査読無し][通常論文]
  • 岡本迪成, 長内俊也, 栗栖宏多, 下田祐介, 中山若樹, 数又研, 鐙谷武雄, 七戸秀夫, 寶金清博 J Neuroendovascular Ther 8 (6) 396 2014年12月01日 [査読無し][通常論文]
  • 小林聡, 宮本倫行, 品田伸一郎, 伊師雪友, 下田祐介, 山崎和義, 牛越聡, 大岡智学, 松居喜郎, 寳金清博 Neurol Surg 42 (11) 1057 -1062 2014年11月10日 [査読無し][通常論文]
  • 神経科学の素朴な疑問 脳梗塞と脾臓は関係がありますか?
    栗栖 宏多, 宝金 清博 Clinical Neuroscience 32 (11) 1313 -1313 2014年11月 [査読無し][通常論文]
  • 脳卒中再生医療 脳梗塞をターゲットとした自己骨髄間質細胞の定位移植治療
    黒田 敏, 七戸 秀夫, 宝金 清博, 出澤 真理 脳循環代謝 26 (1) 103 -103 2014年11月 [査読無し][通常論文]
  • 鐙谷 武雄, 新保 大輔, 七戸 秀夫, 中山 若樹, 数又 研, 宝金 清博 人工血液 22 (1) 33 -33 2014年11月 [査読無し][通常論文]
  • 中山 若樹, 宝金 清博 日本臨床 72 (増刊7 最新臨床脳卒中学(下)) 488 -494 2014年10月 [査読無し][通常論文]
  • 大西浩文, 榊原守, 竹内利治, 三浦哲嗣, 寳金清博, 佐藤園子 北海道医報 (1153) 20 -24 2014年10月01日 [査読無し][通常論文]
  • 加納 崇裕, 佐藤 智香, 長沼 亮滋, 高橋 育子, 松島 理明, 中野 史人, 佐藤 和則, 廣谷 真, 矢部 一郎, 笹森 徹, 関 俊隆, 宝金 清博, 佐々木 秀直 神経治療学 31 (5) 638 -638 2014年09月 [査読無し][通常論文]
  • 新保 大輔, 鐙谷 武雄, 七戸 秀夫, 中山 若樹, 数又 研, 宝金 清博, 石塚 隆伸 脳循環代謝 25 (2) 51 -55 2014年07月 [査読無し][通常論文]
     
    虚血再灌流時に人工酸素運搬体liposome-encapsulated hemoglobin(LEH)を再開通動脈から灌流させることによる脳保護効果について報告する。ラットの一過性中大脳動脈閉塞再灌流モデルを用い、再開通した内頸動脈からLEHの灌流を15分間行い、その脳保護効果について検討した。LEHの投与により、再灌流24時間後の神経機能の改善、脳梗塞および浮腫面積の減少を認めた。LEHはラット赤血球よりも末梢微小血管まで灌流していた。LEHは、粒径が小さいことで狭小化した微小血管まで効果的に酸素を運搬し、微小血管内皮細胞の障害を抑制し、脳内への好中球の浸潤ならびに産生されるmatrix metalloprotease-9の産生を抑えることで脳血液関門の破綻を抑制し、脳保護効果を示すことが考えられた。再開通動脈からのLEHの経動脈的投与は、臨床においても虚血再灌流障害を軽減化する可能性がある。(著者抄録)
  • 山口 秀, 寺坂 俊介, 小林 浩之, 茂木 洋晃, 宝金 清博 日本小児血液・がん学会雑誌 51 (2) 183 -184 2014年06月 [査読無し][通常論文]
  • 関 俊隆, 飛騨 一利, 矢野 俊介, 岩崎 喜信, 青山 剛, 宝金 清博 小児の脳神経 39 (1) 130 -130 2014年04月 [査読無し][通常論文]
  • 数又 研, 武藤 達士, 横山 由佳, 中山 若樹, 七戸 秀夫, 新保 大輔, 斎藤 泰弘, 栗栖 宏多, 宝金 清博 脳血管攣縮 29 27 -30 2014年02月 [査読無し][通常論文]
     
    破裂脳動脈瘤の治療を行った58例を対象に、thermistor catheter挿入によるbedside monitoring systemで得られた体液循環動態の指標について検討した。コイル塞栓術施行群とクリッピング術施行群でcardiac index(CI)、global endodiastolic volume index(GEDI)、extravascular lung water index(EVLWI)の平均値に有意差はなく、GEDIが異常値を示す頻度はクリッピング群で有意に高かった。day 0〜7のGEDIはクリッピング術で正常値下限を下回る頻度が高く、day 8〜14のCIはday 0〜7に比較してクリッピング術により正常値を逸脱する頻度が高かった。たこつぼ心筋を続発した症例は、day 0〜1では循環動態の指標は正常下限で、day 2〜5にcardiac depressionが観察され、回復期を経てday 8〜10に正常化した。回復期にはCIやGEDI値が増加し続け、day 10付近では急速に低下してエコー上の心筋の運動の正常化と一致した。
  • 伊東雅基, 宝金清博, 佐々木秀直, 濱結香, 数又研 ウイリス動脈輪閉塞症の診断・治療に関する研究 平成25年度 研究報告書 3 -8 2014年 [査読無し][通常論文]
  • 新保大輔, 新保大輔, 鐙谷武雄, 七戸秀夫, 中山若樹, 数又研, 宝金清博, 石塚隆伸 脳循環代謝(Web) 25 (2) 51‐55 (J‐STAGE) 2014年 [査読無し][通常論文]
  • 中山若樹, 穂刈正昭, 宮本倫行, 黒田敏, 石川達哉, 宝金清博 日本脳ドック学会総会プログラム・抄録集 23rd 059 2014年 [査読無し][通常論文]
  • 月花正幸, 中山若樹, 小山明彦, 山本有平, 宝金清博 Craniosynostosis研究会プログラム・抄録集 10th 16 -17 2014年 [査読無し][通常論文]
  • 山崎和義, 関俊隆, 飛騨一利, 宝金清博 日本脊髄外科学会プログラム・抄録集 29th 252 2014年 [査読無し][通常論文]
  • 杉山拓, 寳金清博, 数又研, 伊東雅基, 中山若樹 日本臨床 347 -351 2013年12月20日 [査読無し][通常論文]
  • 関 俊隆, 飛騨 一利, 矢野 俊介, 青山 剛, 小柳 泉, 岩崎 喜信, 宝金 清博 脊髄外科 27 (3) 270 -273 2013年12月 [査読無し][通常論文]
     
    High-grade spinal cord glioma 14例(男性10例、女性4例、平均38.5歳)の治療成績について検討した。WHO分類はgrade III:10例、IV:4例で、治療後の経過観察期間は中央値15ヵ月であった。腫瘍の局在は頸髄:3例、頸胸髄:2例、胸髄:6例、胸腰髄:3例、腫瘍摘出度は亜全摘出:2例(grade III・IV各1)、部分摘出:9例(grade III:6,IV:3)、open biopsy:3例(grade III:3)であり、術後は全例に放射線療法を行い、6例(grade III:4,IV:2)にradiation cordotomyを追加し、8例(grade III:6,IV:2)に化学療法を行った。経過観察中に10例が死亡し、生存は2例、不明が2例であった。5年生存率は22.2%(grade III:29.6%、IV:0%)、生存中央値は15ヵ月(grade III:25.5ヵ月、IV:12.5ヵ月)で、いずれもgrade別で有意差はなかった。病変の頸髄への浸潤無し群では有り群に比べ5年生存率、生存中央値が有意に良好で、Cox回帰比例ハザード分析では病変の頸髄への浸潤が予後危険因子とされた。
  • 新保 大輔, 鐙谷 武雄, 七戸 秀夫, 中山 若樹, 数又 研, 石塚 隆伸, 宝金 清博 脳循環代謝 25 (1) 105 -105 2013年11月 [査読無し][通常論文]
  • 内野 晴登, 伊東 雅基, 数又 研, 中山 若樹, 藤間 憲幸, 七戸 秀夫, 鐙谷 武雄, 宝金 清博 脳循環代謝 25 (1) 147 -147 2013年11月 [査読無し][通常論文]
  • 栗栖 宏多, 鐙谷 武雄, 新保 大輔, 下田 祐介, 七戸 秀夫, 中山 若樹, 数又 研, 宝金 清博 脳循環代謝 25 (1) 172 -172 2013年11月 [査読無し][通常論文]
  • 伊東 雅基, 栗栖 宏多, Vuignier Sandra, 数又 研, 中山 若樹, 七戸 秀夫, 志賀 哲, 玉木 長良, 宝金 清博 脳循環代謝 25 (1) 146 -146 2013年11月 [査読無し][通常論文]
  • 下田 祐介, 栗栖 宏多, 穂刈 正昭, 中山 若樹, 数又 研, 宝金 清博 脳循環代謝 25 (1) 130 -130 2013年11月 [査読無し][通常論文]
  • 鐙谷 武雄, 加藤 正仁, 森脇 拓也, 吉野 雅美, 青樹 毅, 今村 博幸, 会田 敏光, 七戸 秀夫, 中山 若樹, 数又 研, 宝金 清博 脳循環代謝 25 (1) 163 -163 2013年11月 [査読無し][通常論文]
  • 淺野 剛, 櫻井 寿郎, 齋藤 久泰, 小林 徹, 竹林 誠治, 瀧澤 克己, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 7 (6) 363 -363 2013年11月 [査読無し][通常論文]
  • 小林 聡, 宮本 倫行, 品田 伸一郎, 伊師 雪友, 下田 祐介, 山崎 和義, 牛越 聡, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 7 (6) 316 -316 2013年11月 [査読無し][通常論文]
  • 淺野 剛, 櫻井 寿郎, 齋藤 久泰, 小林 徹, 竹林 誠治, 瀧澤 克己, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 7 (6) 303 -303 2013年11月 [査読無し][通常論文]
  • 南須原 康行, 佐久嶋 研, 奥原 芳子, 渋谷 かをり, 石川 誠, 宝金 清博 臨床看護 39 (11) 1589 -1592 2013年10月 [査読無し][通常論文]
     
    転倒・転落対策の有効性と業務への影響および実際に経験した転倒・転落における対応内容を調査し、分析した。2012年6月に北海道大学病院の病院勤務をしている看護師を対象にアンケートを実施した。5項目の転倒対策、2011年10月から2012年3月迄に経験した転倒・転落インシデントの有無、経験がある場合に患者家族との対応について質問した。対象看護師は499名で、472名から回答を得ることができた。転倒・転落対策として、理解力が不十分な患者においては「有効」「非常に有効」を合わせると監視モニター、センサーマット、ベッド高・柵の調整が多かった。理解力がある患者に対しては、ベッド高・柵の調整とアセスメントシートが多かった。転倒・転落防止対策全般については、「有効であるがその対策を行う場合にはストレスを感じる」と54%が回答した。転倒・転落についての家族対応を行った看護師は116名であり、家族が医療者への不満を訴えたケースは2件のみであった。
  • 穂刈 正昭, 黒田 敏, 中山 若樹, 宝金 清博, 石川 達哉, 上山 博康 北海道醫學雜誌 = Acta medica Hokkaidonensia 88 (4) 154 -154 2013年09月01日 [査読無し][通常論文]
  • 三上 毅, 大瀧 隼也, 平野 透, 秋山 幸功, 鰐渕 昌彦, 宝金 清博, 三國 信啓 脳卒中の外科 = Surgery for cerebral stroke 41 (4) 264 -268 2013年07月31日 [査読無し][通常論文]
     
    In patients with cerebral aneurysms around the optic nerve, preoperative recognition of the interaction between the optic nerve and the aneurysms will contribute to visual preservation following surgery. The aim of this study was to introduce the value of multimodality fusion imaging in order to visualize the relationship between the aneurysm and the optic nerve.
    Three patients with cerebral aneurysm around the optic nerve underwent computed tomography angiography (CTA) and cycled Fast Imaging Employing Steady-state Acquisition (FIESTA-c) sequence. Fusion imaging of both was created using a workstation (Ziostation; Ziosoft Inc., Tokyo, Japan). We evaluated the preoperative deformation of the optic nerve compressed by the aneurysms using the fusion imaging. All magnetic resonance imaging examinations were performed using a 3.0T Signa HDx scanner (GE Medical Systems Milwaukee, WI, USA) with an 8-channel head coil. A CT examination was performed using a multidetector row CT scanner (GE Light Speed VCT; GE Healthcare, Milwaukee, WI, USA). The optic nerves were distinguished from aneurysms using fusion imaging, and this technique allowed visualization of optic nerve deformation induced by the aneurysm.
    Fusion imaging was useful for demonstrating the optic nerve status when surrounded by aneurysms. It was also useful in establishing a surgical strategy, enhancing intra-operative spatial orientation and increasing surgeon's confidence.
  • Yasuhiro Yonekawa, Kiyohiro Houkin NEUROSURGICAL REVIEW 36 (3) 418 -419 2013年07月 [査読有り][通常論文]
  • 三上 毅, 大瀧 隼也, 平野 透, 秋山 幸功, 鰐渕 昌彦, 宝金 清博, 三國 信啓 脳卒中の外科 41 (4) 264 -268 2013年07月 [査読無し][通常論文]
     
    術前にCT angiography(CTA)を行った未破裂脳動脈瘤79例のうち、視神経近傍の症候性脳動脈瘤に対してcycled FIESTAとのフュージョン画像を作成した3例(全女、61〜74歳)について報告した。内訳は前交通動脈瘤1例、内頸動脈瘤2例で、神経症状としていずれも視野障害を認め、1例は視力障害、外転神経麻痺、動眼神経麻痺もあった。治療内容は、1例でクリッピング術を、2例はproximal ligationおよび橈骨動脈グラフトを用いたhigh flow bypass術を行った。フュージョン画像作成には平均2時間を要した。認識しうる二つのイメージ間の空間的なギャップは認めず、良好にフュージョンされた。2例で視神経の圧迫部位を確認したが、術中所見と合致しており、手術シミュレーションとして有効であった。術後の視力視野障害の悪化はなく、1例は視野の改善、他の1例で視力視野の改善が認められた。
  • 南須原 康行, 佐久嶋 研, 奥原 芳子, 渋谷 かをり, 伊藤 陽一, 石川 誠, 宝金 清博 日本医療マネジメント学会雑誌 14 (1) 25 -30 2013年05月 [査読無し][通常論文]
     
    北海道大学病院における2003年度から2009年度までの7年間にわたるインシデントレポートの中から、看護職によって報告されたものを抽出し、報告者の経験年数、レポート件数、場面などについて分析を行った。7年間で看護職から報告されたインシデントレポート総数は12,798件であった。7対1看護の導入により2007年度に145名の看護職の増員があり、総看護職の増員が22.0%であったのに対し、インシデントレポート件数は33.0%の増加であった。2007年度は総看護職数における1年目看護職数の割合が高く、1年目看護職による報告件数の占める割合が他の年度と比較して高かった。一方、1年目看護職1人あたりの平均年間報告件数の経年変化をみると、2007年度はそれ以前に比べて増加していなかった。看護職の増員は、看護職1人あたりのインシデントレポート件数の減少には繋がらなかった。看護職1年目ではインシデント全体に占める処方・与薬の割合が、2・3年目、4年目以上と比較して有意に大きかった。その内訳では、末梢静脈点滴なかでも投与速度に関するものの割合が、1年目および2・3年目は4年目以上に比べて有意に大きかった。看護職の新人教育においては、新人が関与する可能性の高いインシデントに重点をおいた教育が必要であろう。(著者抄録)
  • 南須原 康行, 佐久嶋 研, 伊藤 陽一, 奥原 芳子, 渋谷 かをり, 石川 誠, 宝金 清博 日本医療マネジメント学会雑誌 14 (1) 31 -36 2013年05月 [査読無し][通常論文]
     
    転倒・転落予防のための病院の注意義務については厳しいものが求められており、入院時に転倒・転落の危険度を予測し、それに基づく説明・対応を行うことが一般的になっている。北海道大学病院でも「転倒・転落アセスメントシート」を用いているが、その有効性などについての検証は行われていない。また、入院時における書類や手続きの増加により、アセスメント自体が看護師の負担にもなっている。そこで、当院で用いられているアセスメントシートの妥当性を検討した。2009年度に当院の脳神経外科・神経内科病棟に入院した成人患者532名を対象とした。転倒・転落の発生率は7.5%(40件)であり、I:2.7%、II:5.3%、III:15.3%と危険度に応じて上昇していた。転倒・転落の有無の2群間で入院期間を考慮したCox単回帰モデルでは、感覚障害、認識力、性格については両群で有意差を認めなかった。また、年齢も有意差を認めなかった。入院期間を考慮したCox重回帰モデルの結果で有意に残ったのは、転倒既往と薬剤使用の2項目であった。転倒の既往も薬剤使用のいずれも該当しない群、どちらか1項目のみ該当する群、両方とも該当する群の3群に分類すると、転倒・転落発生率は、現在使用している8項目に基づく危険度分類によるものとほぼ同等の結果であった。当院の成人用転倒・転落アセスメントシートは、転倒・転落の危険度の予測として概ね妥当であった。(著者抄録)
  • 飛騨 一利, 青山 剛, 矢野 俊介, 宝金 清博 Journal of Spine Research 4 (3) 564 -564 2013年03月 [査読無し][通常論文]
  • Satoshi Kuroda, Naoki Akioka, Daina Kashiwazaki, Hideo Hamada, Naoya Kuwayama, Kiyohiro Houkin STROKE 44 (2) 2013年02月 [査読無し][通常論文]
  • Masaki Ito, Satoshi Kuroda, Koji Takahashi, Masahito Kawabori, Michiyuki Miyamoto, Tomohiro Yamauchi, Hisayasu Saito, Hideo Shichinohe, Kiyohiro Houkin STROKE 44 (2) 2013年02月 [査読無し][通常論文]
  • Saito Hisayasu, Kuroda Satoshi, Miyamoto Michiyuki, Yamauchi Tomohiro, Shichinohe Hideo, Zhao Songji, Magota Keiichi, Kubo Naoki, Kuge Yuji, Tamaki Nagara, Houkin Kiyohiro STROKE 44 (2) 2013年02月 [査読有り][通常論文]
  • 月花正幸, 飛騨一利, 関俊隆, 長内俊也, 内野晴登, 山崎和義, 吉田道春, 宝金清博 日本脊髄外科学会プログラム・抄録集 28th 2013年
  • 七戸秀夫, 杉山拓, 濱内祝嗣, 数又研, 中山若樹, 寳金清博, 黒田敏 脳循環代謝 25 (1) 148 2013年 [査読無し][通常論文]
  • 伊東雅基, 数又研, 中山若樹, 七戸秀夫, 宝金清博 ウイリス動脈輪閉塞症の診断・治療に関する研究 平成24年度 総括・分担研究報告書 6 -12 2013年 [査読無し][通常論文]
  • 宝金清博, 七戸秀夫, 伊東雅基, 数又研, 中山若樹 ウイリス動脈輪閉塞症の診断・治療に関する研究 平成24年度 総括・分担研究報告書 3 -5 2013年 [査読無し][通常論文]
  • 初山 貴, 勝谷 剛, 佐々木 綾香, 横山 瑠奈, 齊藤 恵里佳, 渋谷 かをり, 奥原 芳子, 石川 誠, 南須原 康行, 寶金 清博 診療情報管理 : 日本診療情報管理学会誌 = Health information management 24 (3) 68 -71 2012年12月25日 [査読無し][通常論文]
  • 骨髄間質細胞の動脈内送達の治療効果(Therapeutic effects of intra-arterial delivery of bone marrow stromal cells)
    長内 俊也, 黒田 敏, 七戸 秀夫, 久下 裕司, 玉木 長良, 宝金 清博 脳循環代謝 24 (1) 147 -147 2012年11月 [査読無し][通常論文]
  • 宮本 倫行, 黒田 敏, 趙 松吉, 孫田 恵一, 伊東 雅基, 川堀 真人, 七戸 秀夫, 宝金 清博, 久下 裕司, 玉木 長良 北海道醫學雜誌 = Acta medica Hokkaidonensia 87 (6) 269 -269 2012年11月01日 [査読無し][通常論文]
  • 内野 晴登, 黒田 敏, 平田 健司, 志賀 哲, 宝金 清博, 玉木 長良 北海道醫學雜誌 = Acta medica Hokkaidonensia 87 (6) 278 -278 2012年11月01日 [査読無し][通常論文]
  • 長内 俊也, 牛越 聡, 中山 若樹, 数又 研, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 6 (5) 289 -289 2012年11月 [査読無し][通常論文]
  • 淺野 剛, 宝金 清博, 馬渕 正二, 吉本 哲之, 石井 伸明, 青樹 毅, 小林 徹, 瀧澤 克己 JNET: Journal of Neuroendovascular Therapy 6 (5) 236 -236 2012年11月 [査読無し][通常論文]
  • 笹森 徹, 飛騨 一利, 矢野 俊介, 浅野 剛, 長内 俊也, 青山 剛, 岩崎 喜信, 斉藤 久壽, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 6 (5) 424 -424 2012年11月 [査読無し][通常論文]
  • 七戸秀夫, 寳金清博 Medicina 49 (11) 254-256 2012年11月01日 [査読無し][通常論文]
  • KASHIWAZAKI Daina, TERASAKA Shunsuke, KAMOSHIMA Yuuta, KUBOTA Kanako, ASANO Takeshi, HOUKIN Kiyohiro Neurologia medico-chirurgica = 神経外科 52 (10) 745 -747 2012年10月15日 [査読無し][通常論文]
     
    A 46-year-old man with factor VIII deficiency presented with a rare case of hemophilic pseudotumor in the temporal bone manifesting as severe conductive hearing loss and external ear bleeding. The pseudotumor expanded and destroyed the temporal bone and skin of the external ear over the course of 8 years. The pseudotumor was surgically excised, and the patient's symptoms improved. Histological examination of a specimen collected from inside the pseudotumor demonstrated blood products in various stages of evolution and showed that the outer membrane consisted of a collagen layer. Hemophilic pseudotumors are rare complications occurring in 1-2% of patients with mild or severe hemophilia. Pseudotumors are chronic, slowly expanding, encapsulated cystic masses, and most are located in the long bones and pelvis. The present case suggests that cranial pseudotumor should be considered in the differential diagnosis of cranial lesion in a patient with hemophilia.
  • 伊東雅基, 寳金清博, 斎藤久泰, 新保大輔, 茂木洋晃, 川堀真人, 宮本倫行, 山内朋裕 Neurol Surg 40 (10) 923-945 2012年10月10日 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博 小児の脳神経 37 (5) 347 -351 2012年10月 [査読無し][通常論文]
     
    小児もやもや病の長期予後を改善させるための課題と方策について展望した。特に、早期診断と早期治療、乳幼児の周術期管理、脳血行再建術の適切な術式選択、追加的脳血行再建術、頭痛発作を有する症例へのアプローチの重要性について述べた。(著者抄録)
  • 宝金 清博 医療の質・安全学会誌 7 (Suppl.) 100 -100 2012年10月 [査読無し][通常論文]
  • 南須原 康行, 佐久嶋 研, 奥原 芳子, 渋谷 かをり, 石川 誠, 伊藤 陽一, 宝金 清博 医療の質・安全学会誌 7 (Suppl.) 205 -205 2012年10月 [査読無し][通常論文]
  • SASAMORI Toru, HIDA Kazutoshi, YANO Shunsuke, AOYAMA Takeshi, ASANO Takeshi, KUBOTA Kanako, ITO Manabu, ABUMI Kuniyoshi, IWASAKI Yoshinobu, SAITO Hisatoshi, HOUKIN Kiyohiro Neurologia medico-chirurgica = 神経外科 52 (9) 670 -674 2012年09月15日 [査読無し][通常論文]
     
    A 25-year-old man presented with malignant transformation to malignant peripheral nerve sheath tumor (MPNST) in the cervical spine associated with neurofibromatosis type 1. He presented with a 3-week history of rapidly increasing weakness and numbness in all four extremities. Magnetic resonance (MR) imaging of the cervical spine demonstrated a dumbbell-shaped tumor, which compressed the spinal cord at the C2-3 level. The tumor was excised, mainly within the spinal canal to decompress the spinal cord. The histological diagnosis was benign neurofibroma. Three months after surgery, he rapidly developed progressive tetraparesis and MR imaging revealed marked regrowth of an extradural mass into the spinal canal. At reoperation, the regrown mass in the spinal canal was totally excised. The histological diagnosis revealed MPNST. He underwent radiation therapy, with a total dose of 32 Gy, for approximately 3 weeks after the second surgery, but MR imaging showed tumor regrowth within the spinal canal, and his condition deteriorated. The decision was made to remove the tumor radically, including the involved facet and extradural lesion. Posterior fusion using a pedicle screw was performed one month later. He manifested no additional neurological deficits. He has been free of relapse for 46 months. Radical resection remains the most effective treatment for MPNST, although complete removal with a clear tumor margin is often impossible in practice.
  • S. Kuroda, K. Houkin EUROPEAN JOURNAL OF NEUROLOGY 19 174 -174 2012年09月 [査読無し][通常論文]
  • もやもや病の病態と治療 最新の知見 もやもや病の病態における、循環血液中の血管内皮前駆細胞の意義
    七戸 秀夫, 黒田 敏, 杉山 拓, 寳金 清博 脳循環代謝 23 (2) 106 -106 2012年09月 [査読無し][通常論文]
  • 佐久嶋 研, 南須原 康行, 奥原 芳子, 渋谷 かをり, 伊藤 陽一, 梅木 玲緒奈, 遠藤 晃, 宝金 清博 日本医療マネジメント学会雑誌 13 (Suppl.) 389 -389 2012年09月 [査読無し][通常論文]
  • 大川 聡史, 村上 友宏, 宮田 圭, 飯星 智史, 金子 高久, 小柳 泉, 三國 信啓, 宝金 清博 脳神経外科速報 22 (9) 1057 -1063 2012年09月 [査読無し][通常論文]
     
    過去9年間に診断、加療した脊髄動静脈瘻(SDAVF)16症例中、仙骨部SDAVFの4例(67歳女、65歳男、68歳男、76歳男)を対象に神経放射線学的所見と臨床経過を検討し、画像診断上の特徴を報告した。症状は全例で下肢の筋力低下と感覚障害を認め、3例には膀胱直腸障害があった。治療は血管内治療のみ2例、外科治療のみ1例、併用したのが1例であった。全例何らかの改善が得られたが、膀胱直腸障害は改善しにくい傾向にあり、麻痺の程度が強かった76歳男は下肢不全麻痺の回復が遅かった。画像所見として、脊髄MRIのT2強調画像で、全例に脊髄髄内の高信号域や脊髄周囲のflow voidを認めた。血管造影(CTA)では拡張した脊髄静脈と根髄静脈が描出された。全例、拡張した根髄静脈が仙椎レベルまで存在していた。脊髄血管造影は全例、大腿動脈穿刺で行った。流入動脈は内腸骨動脈の枝が3例、左第4腰動脈の枝が1例であり、拡張した異常流出静脈が仙椎レベルから描出された。これらは67歳女と68歳男では終糸、65歳男では右S2神経根、76歳男では左S1神経根に沿って硬膜内に流入し、脊髄レベルに達していた。この異常流出静脈はCTAで描出された腰仙椎レベルでの拡張した血管と一致した。
  • 三上 毅, 杉野 寿哉, 本間 敏美, 宝金 清博, 三國 信啓 てんかん研究 30 (2) 397 -397 2012年09月 [査読無し][通常論文]
  • 齋藤久泰, 黒田敏, 黒田敏, 宮本倫行, 山内朋裕, 七戸秀夫, 趙松吉, 孫田惠一, 久保直樹, 久下裕司, 寶金清博, 玉木長良 核医学 49 (3) 214 2012年08月31日 [査読無し][通常論文]
  • 【脊椎・脊髄のトラブルシューティング-合併症の予防と対策】脊髄血管内治療における合併症とその対策
    長内 俊也, 飛騨 一利, 牛越 聡, 浅野 剛, 青山 剛, 宝金 清博 脊椎脊髄ジャーナル 25 (8) 793 -799 2012年08月 [査読無し][通常論文]
  • 飛騨 一利, 青山 剛, 矢野 俊介, 長内 俊也, 宝金 清博 北海道整形災害外科学会雑誌 54 (1) 145 -145 2012年08月 [査読無し][通常論文]
  • Satoshi Kuroda, Kiyohiro Houkin, Susumu Miyamoto, Jyoji Nakagawara, Kuniaki Ogasawara, Koji Iihara, Ken-ichiro Kikuta, Teiji Tominaga, Yoshikazu Okada, Norihiro Suzuki, Shigeru Nogawa, Tadashi Nariai, Kiyotaka Fujii, Jun-ichi Ono, Kazuo Yamada, Kazuo Kitagawa, Izumi Nagata, Nobuo Hashimoto JOURNAL OF NEUROSURGERY 117 (2) A411 -A411 2012年08月 [査読無し][通常論文]
  • 宮本 倫行, 黒田 敏, 趙 松吉, 孫田 恵一, 伊東 雅基, 川堀 真人, 七戸 秀夫, 宝金 清博, 久下 裕司, 玉木 長良 核医学 49 (3) S194 -S194 2012年08月 [査読無し][通常論文]
  • 大畑 建治, 佐伯 直勝, 宝金 清博, 吉田 一成 Medical Torch 8 (1) 8 -19 2012年08月 [査読無し][通常論文]
     
    かつて頭蓋底腫瘍は治療が困難な疾患として捉えられていたが、近年の飛躍的な外科手技などの発展により、その治療成績は飛躍的に向上した。なかでも以前は切除できなかった良性脳腫瘍は、根治性・安全性・低侵襲性を求めた治療法へと向かい、治療戦略も変化するなど、ますますエキサイティングな領域になっている。そこで今回は、良性脳腫瘍を中心に「頭蓋底外科の挑戦」をテーマに、この分野のオピニオンリーダーから現状、問題点、今後について語っていただいた。(著者抄録)
  • 茂木 洋晃, 鴨嶋 雄大, 寺坂 俊介, 小林 浩之, 宝金 清博 北海道医学雑誌 87 (4-5) 147 -151 2012年08月 [査読無し][通常論文]
     
    室温保存が可能かつ安価なcollagen-1-coated plateの癌幹細胞単層培養への応用の可否について検討した。ヒト膠芽腫から樹立された細胞株であるU87MGを使用した。培養液はserum contained medium(SCM)とserum free medium(SFM)を用いた。Collagen-1-coated plateではSCM、SFMいずれの培養液でも単層培養の状態であった。Non-coat/SFMの細胞数は有意に低値で、collagen-1-coated plateはNon-coat/SCMと比較してSCM、SFM双方で有意に細胞数は高値であった。SFMで培養したすべてのプレートでCD133の発現はNon-coat/SCMと比較し有意に上昇した。Non-coat/SCMにでは散在性にCD23陽性細胞のみを観察したが、Col/SFMでは多数のCD133陽性細胞数を認めた。
  • 平田 健司, 寺坂 俊介, 志賀 哲, 服部 直也, 孫田 恵一, 小林 浩之, 山口 秀, 宝金 清博, 田中 伸哉, 久下 裕司, 玉木 長良 北海道醫學雜誌 = Acta medica Hokkaidonensia 87 (4) 179 -179 2012年08月01日 [査読無し][通常論文]
  • 川堀 真人, 黒田 敏, 杉山 拓, 七戸 秀夫, 宝金 清博, 久下 裕司, 玉木 長良 北海道醫學雜誌 = Acta medica Hokkaidonensia 87 (4) 205 -205 2012年08月01日 [査読無し][通常論文]
  • 長内 俊也, 黒田 敏, 七戸 秀夫, 杉山 拓, 久下 裕司, 宝金 清博, 玉木 長良, 岩崎 喜信 北海道醫學雜誌 = Acta medica Hokkaidonensia 87 (4) 206 -206 2012年08月01日 [査読無し][通常論文]
  • 長内俊也, 飛騨一利, 浅野剛, 青山剛, 寳金清博 Neurol Surg 40 (8) 695 -703 2012年08月 [査読無し][通常論文]
     
    PURPOSE: Multi-detector-row CT angiography (MDCTA) is useful for diagnosis of spinal arteriovenous (AV) shunt. So we reported the role and pitfall of MDCTA based on our experience. SUBJECTS: From January 2007 to September 2011, 57 cases used MDCTA in our institution. We investigated 22 case of spinal dural AVF and 5 cases of epidural AVF. METHODS: All cases were performed by Toshiba Aquilion 64. FOV was the whole spine, curved planar reconstruction along the spinal canal, sagittal and axial view obtained by multi-planar reconstruction. RESULTS: MDCTA detected dilated the perimedullary vein in all cases and identified the level of segmental artery matching the result of DSA in 16 cases of spinal dural AVF (73%) and 1 cases of epidural AVF (20%). CONCLUSION: MDCTA is useful for screening, and identifying the shunt point, but care needs to be taken concerning the particular pitfall of MDCTA.
  • 川堀 真人, 数又 研, 大西 浩介, 杉山 拓, 伊東 雅基, 中山 若樹, 寳金 清博 Neurological Surgery 40 (8) 731 -740 2012年08月 [査読無し][通常論文]
     
    2000〜2011年の未破裂中大脳動脈瘤の手術成績が報告されている論文をPubMed(英文)、J-stage(和文)により検索し、開頭clipping術に関する21件1323例を抽出し解析した。21件のcase seriesにおけるmorbidityは0.0〜26.1%に分布していた。開頭術により合併症を呈した例は54例集積され、平均4.1%と算出された。瘤サイズ別では7mm以下が1.48%、15mm前後が5.93%、25mm以上が27.8%で、死亡は1例のみであった。中大脳動脈領域の虚血性合併症の出現率は2.1%、穿通枝領域では1.3%、術後1週間以降に生じたvasospasmは0.7%、静脈性梗塞は0.7%であった。巨大動脈瘤、血栓化動脈瘤、serpentine aneurysm、コイル塞栓術後の再処理などのcomplex aneurysmsは64例報告され、合併症は15例(23.4%)にみられた。稀な合併症としてはclipに関連するslip out、voaospasm、動脈瘤再増大・血栓化、ビオボンドによる遅発性動脈閉塞などが報告されていた。
  • 齋藤久泰, 中山若樹, 瀧川修吾, 牛越聡, 新保大輔, 黒田敏, 寳金清博 Neurol Surg 40 (8) 717 -722 2012年08月 [査読無し][通常論文]
     
    Isolated abducens nerve palsies associated with the rupture of intracranial aneurysms have rarely been reported. We report two cases of isolated bilateral abducens nerve palsies occurring after subarachnoid hemorrhage due to the rupture of an intracranial aneurysm. Case 1 : A 49-year-old woman had bilateral abducens nerve palsies following subarachnoid hemorrhage due to the rupture of the left vertebral artery-posterior inferior cerebellar artery aneurysm. Case 2: A 55-year-old man had bilateral abducens nerve palsies following subarachnoid hemorrhage due to dissecting aneurysm of the right vertebral artery. Case 1 and 2 were treated with surgical clipping of the aneurysm and internal occlusion of the parent artery. In both cases, bilateral abducens nerve palsies achieved almost full recovery several months after treatment. It is speculated that the main causes of palsies are compression and stretching of the bilateral abducens nerves by a thick clot in the prepontine cistern. Although most of the abducens palsies may be reversible and have good prognosis, it is important that they are kept in mind as isolated symptoms of subarachnoid hemorrhage.
  • 穂刈正昭, 長内俊也, 中山若樹, 濱内祝嗣, 伊藤康裕, 下田祐介, 寳金清博 Neurol Surg 40 (7) 651-656 2012年07月10日 [査読無し][通常論文]
  • 【イメージテクノロジーの進歩と脳卒中治療】脳循環代謝イメージングの進歩 ASL法によるもやもや病術後過灌流の診断
    杉野 寿哉, 飯星 智史, 三上 毅, 長濱 宏史, 原田 邦明, 寶金 清博, 三國 信啓 The Mt. Fuji Workshop on CVD 30 51 -55 2012年07月 [査読無し][通常論文]
     
    血行再建術を施行したもやもや病20例21側(男11名、女9名、4〜60歳)を対象に、術後急性期にASL(動脈スピンラベル)法と123I-IMP-SPECTを併用し過灌流評価を行った。ASL法はFAIRによる評価が15例15側、pCASLによる評価が5例6側であった。過灌流症候群を呈したのは3例で、FAIRでの評価が2例、pCASLでの評価は1例であった。FAIRとSPECTの術側/非術側(O/N)比は相関があり、過灌流症候群を呈した2例はFAIRとSPECTのO/N比は増加しており、過灌流の検出が可能であった。また、pCASLで過灌流症候群を呈した1例でもpCASLとSPECTのO/N比は増加しており、過灌流の検出が可能であった。ASL法はもやもや病に対する血行再建術後の過灌流評価に有用であると考えられた。
  • 長内俊也, 浅野剛, 寺坂俊介, 寳金清博 Neurol Surg 40 (7) 599 -606 2012年07月 [査読無し][通常論文]
     
    Preoperative embolization for hypervascular tumors is typically performed using particle agents such as polyvinyl alcohol, gelfoam powder, and fibrin glue. Furthermore, n-butyl-2-cyanoacrylate (NBCA) is used in patients with cerebral arteriovenous malformation (AVM) or an AV shunt. However, few reports have described the efficacy of NBCA in treating hypervascular tumors. Here, we report cases of hypervascular tumors in which preoperative embolization was performed using NBCA. We also discuss the difference between the efficacy of a liquid agent and a particle agent for hypervascular tumor embolization. We analyzed 10 cases encountered at our institution since 2004 in which preoperative embolization was performed using NBCA. In all cases, NBCA was injected through the tumor-feeding artery. In eight of these cases, preoperative embolization decreased intraoperative bleeding and markedly reduced the tumor stain. In the remaining two cases, complications occurred but without any permanent sequel. Thus, NBCA is useful for preoperative embolization.
  • 鈴木健吾, 鰐渕昌彦, 秋山幸功, 池田潤, 南田善弘, 長谷川匡, 寳金清博, 三國信啓 Neurol Surg 40 (7) 617 -621 2012年07月 [査読無し][通常論文]
     
    We described a case of primary clear cell carcinoma (CCC) of the paranasal cavity and skull base. A 59-year-old female experienced chronic nasal obstruction and double vision. Computed tomography (CT) scan revealed a mass lesion of the paranasal cavity, reaching the skull base and intracranial zone. We performed a biopsy by endonasal endoscopic surgery and pathological examinations revealed the tumor was a clear cell carcinoma. No primary tumor was identified on evaluation of the whole body by CT scan and FDG-PET ( 18fluorodeoxy glucose positron emission tomography) scan, so we diagnosed a primary CCC of the paranasal cavity. We decided to use stereotactic radiation therapy. Neurologically, her diplopia was improved. The patient is well at 2 years of follow-up with no evidence of local enlargement. There are some reports about the metastatic sinonasal CCC, but there are few reports about primary nasal and sinonasal CCC. This is the fourth case report of primary nasal and sinonasal CCC.
  • 浅野剛, 黒田敏, 寳金清博, 吉田大介, 長和俊, 白石秀明, 齋藤伸治 Neurol Surg 40 (6) 519 -525 2012年06月10日 [査読無し][通常論文]
     
    We report a case of vein of Galen aneurysmal malformation (VGAM) with a newly developed dural arteriovenous fistula (AVF) subsequent to successful embolization. A male neonate diagnosed as VGAM with prenatal ultrasonography and MRI presented severe cardiac and respiratory failure soon after birth. Five sessions of transarterial embolization using NBCA were performed during the first 6 months of his life. The shunt flow was effectively reduced and heart failure was resolved after the treatment. Follow-up angiography performed 2.5 years after the last embolization revealed complete obliteration of VGAM and newly developed small dural AVF on the wall of the thrombosed falcorial sinus. We believe that the dural AVF in this case was caused by local venous hypertension or induction of angiogenic factor during the thrombosing process of VGAM.
  • 痙縮に対して髄腔内バクロフェン療法が奏効した3症例
    加納 崇裕, 矢部 一郎, 佐々木 秀直, 保前 英希, 青山 剛, 宝金 清博 臨床神経学 52 (6) 459 -459 2012年06月 [査読無し][通常論文]
  • 川堀 真人, 新谷 好正, 栗栖 宏多, 太田 穣, 馬渕 正二, 宝金 清博 未病と抗老化 21 130 -133 2012年06月 [査読無し][通常論文]
     
    脳ラクナ梗塞は穿通動脈の閉塞によって運動障害等をもたらす重大な疾患であり、その発症機序に関して高血圧の関与が一番大きいことが知られているが、実際ラクナ梗塞患者に占める未治療高血圧の頻度は不明である。そこで今回我々は北海道後志地区の唯一の脳疾患拠点病院である当院において初発脳梗塞患者における未治療高血圧と他のリスクファクターの頻度をretrospectiveに検討し、その一次予防の重要性について考察した。症例は2010年1月から10月までの10ヵ月間に当院脳神経外科に入院した615人のうち、初発脳ラクナ梗塞患者50人を対象とした。その内訳は男性26人・女性24人で平均年齢70.0±12.3歳であった。初発症状は軽度麻痺が62%、構音障害が20%、その他眼球運動障害、しびれなどであった。退院時のmodified Rankin Scale(mRS)はgood(mRS≦3)が86%であったが、poor(mRS≧4)が14%存在した。リスクファクターの頻度は高血圧85%、糖尿病34%、高脂血症32%、喫煙42%であり、特に高血圧を有する患者の55%が未治療(健康診断未受診、自己判断での通院中断等)であった。高血圧未治療患者群と治療患者群で退院時mRSに有意差はなかった。脳ラクナ梗塞は高血圧がその発症に強く関与していることがしられているが、半数以上の初発脳ラクナ梗塞患者において高血圧が未治療であることが示された。高齢社会を迎えている現在、このような脳梗塞高リスク患者をどのようにしてスクリーニングし、継続受診させていくかは今後の課題である。(著者抄録)
  • 杉山 拓, 寳金 清博, 伊東 雅基, 穂刈 正昭, 中山 若樹, 数又 研, 黒田 敏 Neurological Surgery 40 (6) 555 -565 2012年06月 [査読無し][通常論文]
  • 齋藤久泰, 黒田敏, 宮本倫行, 山内朋裕, 新保大輔, 七戸秀夫, 趙松吉, 孫田惠一, 久保直樹, 久下裕司, 玉木長良, 寶金清博 再生医療 11 191 2012年05月16日 [査読無し][通常論文]
  • 中間型松果体実質腫瘍の臨床病理学的検討
    伊東 民雄, 佐藤 憲市, 及川 光照, 中村 博彦, 寺坂 俊介, 宝金 清博, 田中 伸哉, 菅野 宏美, 久保田 佳奈子, 村田 純一 Brain Tumor Pathology 29 (Suppl.) 176 -176 2012年05月 [査読無し][通常論文]
  • 4年の経過で髄腔外転移を認めたanaplastic ependymoma
    金子 貞洋, 小林 浩之, 寺坂 俊介, 鴨嶋 雄大, 茂木 洋晃, 遠藤 将吾, 久保田 佳奈子, 宝金 清博 Brain Tumor Pathology 29 (Suppl.) 206 -206 2012年05月 [査読無し][通常論文]
  • 両側性Lhermitte-Duclos diseaseの一例
    鴨嶋 雄大, 寺坂 俊介, 小林 浩之, 内野 晴人, 久保田 佳奈子, 村田 純一, 宝金 清博 Brain Tumor Pathology 29 (Suppl.) 227 -227 2012年05月 [査読無し][通常論文]
  • 不妊治療開始後に出血にて発症したラブドイド髄膜腫の一例
    茂木 洋晃, 小林 浩之, 寺坂 俊介, 石井 伸明, 伊東 雅基, 新保 大輔, 久保田 佳奈子, 宝金 清博 Brain Tumor Pathology 29 (Suppl.) 231 -231 2012年05月 [査読無し][通常論文]
  • 淺野 剛, 飛騨 一利, 長内 俊也, 青山 剛, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 6 (1) 32 -39 2012年05月 [査読無し][通常論文]
     
    【目的】多発頸髄硬膜動静脈瘻を伴った頸部巨大傍椎体動静脈シャントに対し,塞栓術を行い良好な症状コントロールを得た症例を経験したので報告する.【症例呈示】47歳男性,進行性の脊髄症状にて発症.左頸椎前方の巨大傍椎体動静脈シャント,左後頸肩部のsoft tissue arteriovenous malformation(AVM)を認めspinal arteriovenous metameric syndrome(SAMS)と考えられた.傍椎体動静脈シャントに対し経動脈および経静脈的塞栓術を行ったが症状は改善せず,経過中に二次性の頭蓋頸椎移行部からC2レベルの多発硬膜動静脈瘻の存在が明らかとなり,これが脊髄症状の主因と考えられた.多発硬膜動静脈瘻に対する経動脈的塞栓術の結果,症状改善が得られた.【結論】SAMSに代表される複雑かつ高流量の脊髄・脊椎動静脈シャントの治療においては,主訴に対する責任病変を正確に認識し,その形態を十分に評価した上で計画的に治療を行うことが重要である.(著者抄録)
  • 宝金 清博 脳神経外科ジャーナル 21 (0) 72 -72 2012年04月20日 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博 小児の脳神経 37 (2) 128 -128 2012年04月 [査読無し][通常論文]
  • 小児トルコ鞍部黄色肉芽腫5症例の検討(Xanthogranuloma of the sellar region of children: series of five cases and literature review)
    鴨嶋 雄大, 澤村 豊, 寺坂 俊介, 小林 浩之, 久保田 佳奈子, 宝金 清博 小児の脳神経 37 (2) 157 -157 2012年04月 [査読無し][通常論文]
  • 伊藤 康裕, 黒田 敏, 小山 明彦, 内野 晴登, 山崎 和義, 濱内 祝嗣, 下田 佑介, 宝金 清博 小児の脳神経 37 (2) 191 -191 2012年04月 [査読無し][通常論文]
  • 宝金 清博, 嘉山 孝正, 大野 喜久郎, 鈴木 倫保, 新井 一, 伊達 勲, 中瀬 裕之, 藤井 幸彦, 寺本 明, 日本脳神経外科学会医療安全管理委員会 日本医事新報 0 (4591) 25 -29 2012年04月 [査読無し][通常論文]
  • 内野 晴登, 中村 俊孝, 黒田 敏, 中山 若樹, 宝金 清博, 村田 純一, 斎藤 久寿 北海道医学雑誌 87 (2-3) 83 -83 2012年04月 [査読無し][通常論文]
  • 杉野 寿哉, 三上 毅, 飯星 智史, 寶金 清博, 三國 信啓 脳卒中の外科 = Surgery for cerebral stroke 40 (2) 89 -93 2012年03月31日 [査読無し][通常論文]
     
    著者らの施設でもやもや病に対し血行再建術を施行した10例(男女各5例、年齢4〜49歳、平均年齢27.8歳)を対象に、中硬膜動脈の温存方法について紹介した。手術は直接血行再建のSTA-MCA吻合術に加え、間接血行再建としてEDMSおよびEDASが施行された。その結果、中硬膜動脈の頭蓋骨貫通部を顕微鏡下にドリルで骨切除することにより、全例で中硬膜動脈を温存することが可能で、術中ICGでも中硬膜動脈の開通を確認できた。また、術後急性期の頭部MRA、CTAでも中硬膜動脈の開存が確認された。
  • 青山 剛, 飛騨 一利, 矢野 俊介, 浅野 剛, 長内 俊也, 宝金 清博 Journal of Spine Research 3 (3) 566 -566 2012年03月 [査読無し][通常論文]
  • 当院における処方時の相互作用警告機能の実際と検証
    南須原 康行, 佐久嶋 研, 遠藤 晃, 梅木 玲緒奈, 沖 洋充, 山田 武宏, 井関 健, 石川 誠, 宝金 清博 日本予防医学リスクマネージメント学会学術総会プログラム・抄録集 10回 100 -100 2012年03月 [査読無し][通常論文]
  • 黒田 敏, 杉山 拓, 川堀 真人, 笹森 徹, 栗栖 宏多, 内野 晴登, 七戸 秀夫, 中山 若樹, 宝金 清博 脳卒中の外科 40 (2) 77 -82 2012年03月 [査読無し][通常論文]
  • 伊師雪友, 山崎和義, 栗栖宏多, 濱内祝嗣, 下田祐介, 浅野剛, 長内俊也, 小林浩之, 寺坂俊介, 寳金清博 日本脳腫瘍の外科学会プログラム・抄録集 17th 2012年
  • Kiyohiro Houkin CEREBROVASCULAR DISEASES 34 104 -104 2012年 [査読無し][通常論文]
  • Takeshi Mikami, Toshiya Sugino, Shunya Ohtaki, Houkin Kiyohiro, Nobuhiro Mikuni CEREBROVASCULAR DISEASES 34 105 -105 2012年 [査読無し][通常論文]
  • Saito Hisayasu, Kuroda Satoshi, Nakayama Naoki, Hirata Kenji, Magota Keiichi, Shiga Tohru, Tamaki Nagara, Yoshida Daisuke, Terae Satoshi, Kazumata Ken, Houkin Kiyohiro CEREBROVASCULAR DISEASES 34 40 -41 2012年 [査読有り][通常論文]
  • 淺野 剛, 飛騨 一利, 長内 俊也, 青山 剛, 宝金 清博 脳神経血管内治療 6 (1) 32 -39 2012年 [査読無し][通常論文]
     
    【目的】多発頚髄硬膜動静脈瘻を伴った頚部巨大傍椎体動静脈シャントに対し，塞栓術を行い良好な症状コントロールを得た症例を経験したので報告する．【症例呈示】47歳男性，進行性の脊髄症状にて発症．左頚椎前方の巨大傍椎体動静脈シャント，左後頚肩部のsoft tissue arteriovenous malformation（AVM）を認めspinal arteriovenous metameric syndrome（SAMS）と考えられた．傍椎体動静脈シャントに対し経動脈および経静脈的塞栓術を行ったが症状は改善せず，経過中に二次性の頭蓋頚椎移行部からC2レベルの多発硬膜動静脈瘻の存在が明らかとなり，これが脊髄症状の主因と考えられた．多発硬膜動静脈瘻に対する経動脈的塞栓術の結果，症状改善が得られた．【結論】SAMSに代表される複雑かつ高流量の脊髄・脊椎動静脈シャントの治療においては，主訴に対する責任病変を正確に認識し，その形態を十分に評価した上で計画的に治療を行うことが重要である．
  • 宝金 清博, 長内 俊也, 伊東 雅基, 中山 若樹, 黒田 敏 脳卒中の外科 40 (1) 1 -6 2012年01月 [査読無し][通常論文]
     
    本邦の脳卒中治療における外科治療と血管内治療の現状を把握するため、日本脳卒中の外科学会の正会員(2011年4月1日時点)に対してアンケート調査を行った。3021名中1043名から得た回答を分析した結果、脳外科医師数はここ10年間でほぼ変わらず、脳神経外科の病床数は減少傾向にあったが、脳神経外科手術数は増加しており、血管内治療は約2倍に増加していた。殊に未破裂脳動脈瘤や破裂脳動脈など、脳血管障害関連の手術対象疾患のすべてで血管内治療の件数が増加しており、若手医師を中心に42%が脳血管内治療専門医の取得を考えていたが、指導者不在などが専門医取得の障壁となっていた。自由記載からは、今後の脳卒中の外科のあり方に対する関心の高さが伺われ、血管内治療専門医の育成・制度設計に関して議論を進めていく必要があると考えられた。
  • 三上 毅, 鰐渕 昌彦, 飯星 智史, 宮田 圭, 宝金 清博, 三國 信啓 脳卒中の外科 40 (1) 45 -48 2012年01月 [査読無し][通常論文]
     
    6歳男児。多動症精査中に、MRI T2強調画像にて橋前槽に最大径26mmの腫瘤を認め、MRAで脳底動脈瘤と診断された。術前画像診断では上小脳動脈分岐部より中枢側に存在する動脈瘤頸部は狭くて明瞭であり、動脈瘤の血栓化も認めなかったため、左前頭側頭開頭で頸部クリッピングを行った。術後一過性に動眼神経麻痺が出現したが、数日間で改善し、神経学的に異常所見なく独歩退院した。小児脳動脈瘤は合併する疾患が多く、術前の小児科的な精査が不可欠であるが、神経症状の回復は比較的良好なため、十分な展開が可能と思われた。
  • 宝金 清博 脳卒中の外科 = Surgery for cerebral stroke 39 (6) 462 -464 2011年11月30日 [査読無し][通常論文]
  • KAMOSHIMA Yuuta, SAWAMURA Yutaka, IMAI Tetsuaki, FURUKAWA Hiroshi, KUBOTA Kanako, HOUKIN Kiyohiro Neurologia medico-chirurgica = 神経外科 51 (11) 798 -800 2011年11月15日 [査読無し][通常論文]
     
    A 2-year-old female presented with a rare case of recurrent giant cell tumor affecting the frontal bone. She had already undergone partial removal twice at the ages of 14 and 18 months. The tumor was located in the frontal bone, expanding to the ethmoid and orbital bones, and invading the frontal base dura mater. The tumor was totally removed including the surrounding bone and frontal base dura mater. No local recurrence and metastasis were observed at 18 months after the last operation. Most giant cell tumors occur in the epiphyses of long bones and are rare in the cranio-facial bone. These tumors usually affect young adults and few pediatric cases are reported.
  • 淺野 剛, 飛騨 一利, 長内 俊也, 青山 剛, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 5 (4) 168 -168 2011年11月 [査読無し][通常論文]
  • 淺野 剛, 黒田 敏, 宝金 清博, 長 和俊, 吉田 大介, 白石 秀明, 斎藤 伸治 JNET: Journal of Neuroendovascular Therapy 5 (4) 176 -176 2011年11月 [査読無し][通常論文]
  • 長内 俊也, 浅野 剛, 寺坂 俊介, 飛騨 一利, 宝金 清博, 鐙 邦芳, 伊東 学, 磯部 正則, 野村 三起夫 JNET: Journal of Neuroendovascular Therapy 5 (4) 389 -389 2011年11月 [査読無し][通常論文]
  • 抗加齢医療としての再生医学 骨髄細胞を用いた神経疾患治療の展望(神経再生療法の今後と展望)
    宝金 清博 Anti-aging Science 3 (Suppl.1) 314 -314 2011年11月 [査読無し][通常論文]
  • 七戸 秀夫, 黒田 敏, 杉山 拓, 寳金 清博 脳循環代謝 23 (1) 97 -97 2011年11月 [査読無し][通常論文]
  • Kashiwazaki Daina, Ushikoshi Satoshi, Asano Takeshi, Osanai Toshiya, Kuroda Satoshi, Houkin Kiyohiro Acta Neurochirurgica 153 (11) 2151 -2158 2011年11月 [査読無し][通常論文]
     
    To discuss and summarize the strategies and complications of endovascular embolization for aneurysms of the posterior cerebral artery (PCA). Data of patients with PCA aneurysms treated by an endovascular procedure were analyzed retrospectively (n = 21). Twenty patients with aneurysms were treated by detachable coil embolization, and one patient was treated with n-butyl cyanoacrylate. Of the 21 aneurysm embolization cases, 9 were treated by parent artery occlusion (PAO), and 12 were treated by selective occlusion of the aneurysm (SOA). All 12 aneurysms treated by SOA showed complete occlusion. Two aneurysms became recanalized 6 months after the first embolization and were then re-embolized; complete healing was observed on follow-up angiography. All patients showed acceptable outcomes without any procedural complications, except one patient who died 2 days after treatment. PAO resulted in 100% occlusion of all aneurysms. Cerebral infarction was noted in most patients (78%, n = 7). However, the area of infarction was small. Permanent neurological deficit was observed in two patients (22%), but their condition was not critical. Aneurysm embolization with SOA is well indicated for saccular aneurysms with well-defined necks, whereas PAO carries a risk of ischemic complications. Although the PCA is rich in collateral circulation, ischemic complications were noted in most patients after PAO, and it was difficult to predict occurrence of these complications. However, the area of cerebral infarction tended to be small, and the neurological deficits observed were not critical.
  • KAMOSHIMA Yuuta, SAWAMURA Yutaka, MOTEGI Hiroaki, KUBOTA Kanako, HOUKIN Kiyohiro Neurologia medico-chirurgica = 神経外科 51 (10) 689 -693 2011年10月15日 [査読無し][通常論文]
     
    Five Japanese children presented with rare xanthogranuloma located in the sellar region between 2000 and 2010 at the Department of Neurosurgery, Hokkaido University Hospital. Endocrinological examination disclosed central diabetes in four patients. Preoperative magnetic resonance (MR) imaging and computed tomography (CT) demonstrated clearly defined intra- or suprasellar masses appearing as isointense or hyperintense on T1-weighted MR imaging with no calcification on CT. The tumor was totally removed under preoperative diagnosis of craniopharyngioma in all cases. Histological examination found fibrous tissue with abundant cholesterol clefts and hemosiderin deposits, but no or only tiny amounts of epithelial cells in each case. Therefore, the histological diagnoses were xanthogranuloma of the sellar region, not adamantinomatous craniopharyngioma. Postoperatively, no patient recovered from endocrinological deficits, whereas visual disturbances were improved immediately after operation. Currently, whether xanthogranuloma is distinct from adamantinomatous craniopharyngioma remains unclear. Only a few clinical reports of xanthogranuloma of the sellar region have been reported in pediatric patients. Our series shows that xanthogranuloma should be included in the differential diagnosis of pediatric tumor of the sellar region.
  • 当院における成人用転倒・転落アセスメントシートの妥当性についての検討
    南須原 康行, 佐久嶋 研, 伊藤 陽一, 奥原 芳子, 渋谷 かをり, 石川 誠, 寶金 清博 医療の質・安全学会誌 6 (Suppl.) 176 -176 2011年10月 [査読無し][通常論文]
  • 急性疾患における終末期医療の対応と課題 脳神経外科医からみた急性疾患における終末期医療
    宝金 清博 日本医学会総会会誌 28回 (II) 152 -152 2011年10月 [査読無し][通常論文]
  • Kiyohiro Houkin NEUROSURGICAL REVIEW 34 (4) 522 -522 2011年10月 [査読無し][通常論文]
  • Elke R. Gizewski, Kiyohiro Houkin, Marc Sindou NEUROSURGICAL REVIEW 34 (4) 495 -496 2011年10月 [査読無し][通常論文]
  • 加納 崇裕, 白井 慎一, 大原 宰, 竹内 朗子, 堀内 一宏, 秋本 幸子, 矢部 一郎, 佐々木 秀直, 青山 剛, 宝金 清博 神経治療学 28 (5) 542 -542 2011年09月 [査読無し][通常論文]
  • 顔面・頭頸部AVMに対する塞栓術
    浅野 剛, 宝金 清博, 古川 洋志, 佐々木 了 IVR: Interventional Radiology 26 (3) 344 -344 2011年08月 [査読無し][通常論文]
  • Takeshi Aoyama, Kazutoshi Hida, Kiyohiro Houkin NEUROLOGIA MEDICO-CHIRURGICA 51 (8) 561 -566 2011年08月 [査読無し][通常論文]
     
    Magnetic resonance imaging has increased the incidence of the diagnosis of intramedullary cavernous angioma. Surgical therapy tends not to be recommended for asymptomatic lesions, but symptomatic lesions that bleed recurrently should be treated. The natural course of intramedullary cavernous angioma remains unknown and arguments have been raised against the surgical treatment of symptomatic lesions. We reviewed the clinical features of 13 intramedullary cavernous angiomas in 12 patients surgically treated between 1988 and 2009. The 7 men and 5 women were aged from 14 to 60 years, the preoperative interval ranged from 0 to 161 months, and the mean number of hemorrhages in the 13 lesions was 2.5. Sixteen operations were performed to treat the 13 lesions. The surgical approach depended on the lesion location. The outcome of patients with mild to moderate preoperative symptoms (McCormick grades I-III) was significantly better than that of patients with severe symptoms (McCormick grade IV) (p < 0.05). Symptomatic intramedullary cavernous angioma tends to bleed repeatedly. The lesion should be surgically removed to avoid further deterioration due to recurrent hemorrhages. The shortest path approach should be selected based on preoperative images and complete removal should be attempted. Residual lesion may be masked by surrounding gliosis, so careful postoperative follow up is necessary.
  • 金子 貞洋, 寺坂 俊介, 小林 浩之, 宝金 清博, 久保田 佳奈子 脳神経外科ジャーナル 20 (8) 612 -617 2011年08月 [査読無し][通常論文]
  • 小林浩之, 寺坂俊介, 中山若樹, 浅岡克行, 村田純一, 宝金清博 日本脳腫瘍の外科学会プログラム・抄録集 16th 109 2011年07月31日 [査読無し][通常論文]
  • 鴨嶋雄大, 寺坂俊介, 寶金清博, 黒田敏, 中山若樹, 小林浩之, 浅野剛 日本脳腫瘍の外科学会プログラム・抄録集 16th 112 2011年07月31日 [査読無し][通常論文]
  • Yuuta Kamoshima, Yutaka Sawamura, Taku Sugiyama, Shigeru Yamaguchi, Kiyohiro Houkin, Kanako Kubota NEUROLOGIA MEDICO-CHIRURGICA 51 (7) 527 -530 2011年07月 [査読無し][通常論文]
     
    A 55-year-old woman presented with an extremely rare primary central nervous system mucosa-associated lymphoid tissue (MALT) lymphoma manifesting as seizure and was subsequently diagnosed with dural MALT lymphoma in the cranium. Magnetic resonance imaging revealed a left frontal dural mass with peritumoral edema. Histological examination of the dural mass confirmed MALT lymphoma and revealed infiltration of small cells with irregular nuclear borders and expression of a B cell marker (CD20) but absence of CD5, CD10, CD23, and cyclinD1. Reactive T-cell infiltration was also seen. Subsequently, local irradiation (40 Gy/20 fractions) was performed. Magnetic resonance imaging showed complete remission just after irradiation was completed. There was no evidence of systemic MALT lymphoma. There has been no recurrence for 3 years without additional therapy.
  • 森田 明夫, 橋本 信夫, 福原 俊一, 宝金 清博, 野崎 和彦, 池田 俊也, 中山 健夫, 片岡 大治, 西村 雅樹, 長嶺 義秀, 木村 俊運, 徳永 浩司, 秋山 幸功, 北川 直毅, 林 健太郎, 栗田 浩樹, 丸山 啓介, UCASII研究者グループ 脳神経外科ジャーナル 20 (7) 484 -490 2011年07月 [査読無し][通常論文]
     
    国際未破裂脳動脈瘤調査IIにおける治療成績を調査した。対象は2006年に31の参加施設から新規に登録された1059例・1230動脈瘤で、平均年齢61歳、女性が男性の2倍、瘤の平均サイズ6mm、多発性脳動脈瘤13%、ブレブを有する動脈瘤22%であった。実際の治療は558例に行われ、開頭クリッピング81%、血管内治療19%で、治療症例のうちmodified Ranking scale(mRS)が2ポイント以上低下したのは25例認め、mRSのみによる合併症は4.5%であった。また、術前・後のmini mental state examination(MMSE)登録のある247例において、術後MMSEが24以下に低下したのは9例で、うちMMSEとmRSを合わせた重篤合併症例は5.3%であった。mRSに影響した因子についてみると、クモ膜下出血に併発した未破裂脳動脈瘤、10mm以上の瘤、椎骨脳底動脈瘤、血管内治療症例で有意に合併症率が高かった。QOLについては、SF-8の身体・精神スコア、SF-36のmentalヘルス・vitalityスコアのいずれも術前後で大きな変化は認めなかった。治療にかかった経費は、開頭クリッピングに比べ血管内治療で有意に高額であった。
  • 黒田 敏, 宝金 清博 実験医学 29 (10) 1665 -1668 2011年06月 [査読無し][通常論文]
     
    脳梗塞は高血圧、糖尿病、脂質代謝異常などに起因する頭蓋内外の動脈硬化や、心房細動に代表される不整脈によって発生するとされてきたが、近年の研究によって慢性の炎症機転が脳梗塞の発症や再発に深く関与していることが明らかとなりつつある。動脈硬化の発生や進展には炎症や感染が重要な役割を担っており、これをターゲットとした診断法や治療法の開発が待たれている。(著者抄録)
  • High grade meningiomaの治療成績とMib-1 indexの関係の検討
    成田 拓人, 菅野 宏美, 湯澤 明夏, 寺坂 俊介, 小林 浩之, 村田 純一, 山口 秀, 西原 広史, 宝金 清博, 田中 伸哉 Brain Tumor Pathology 28 (Suppl.) 092 -092 2011年05月 [査読無し][通常論文]
  • 画像と病理所見から多中心性神経膠腫と診断した一例
    茂木 洋晃, 寺坂 俊介, 小林 浩之, 鴨嶋 雄大, 山口 秀, 遠藤 将吾, 宝金 清博, 高阪 真路, 田中 伸哉, 村田 純一 Brain Tumor Pathology 28 (Suppl.) 123 -123 2011年05月 [査読無し][通常論文]
  • 原発性CNS粘膜関連リンパ組織リンパ腫(Primary CNS mucosa-associated lymphoid tissue lymphoma)
    鴨嶋 雄大, 澤村 豊, 寺坂 俊介, 杉山 拓, 小林 浩之, 山口 秀, 茂木 洋晃, 久保田 佳奈子, 宝金 清博 Brain Tumor Pathology 28 (Suppl.) 137 -137 2011年05月 [査読無し][通常論文]
  • 神経皮膚黒色症と鑑別を要した巨大色素性有毛性母斑と星細胞腫が合併した稀な1例
    鴨嶋 雄大, 澤村 豊, 斉藤 伸治, 川堀 真人, 寳金 清博, 久保田 佳奈子 Neurological Surgery 39 (5) 479 -483 2011年05月 [査読無し][通常論文]
     
    症例は6歳女児で、生下時より前額部・背部・左腕・両下肢に多発有毛性母斑を認め、神経皮膚黒色症疑いで母斑切除術を受けた。1歳時に当院小児科で行ったMRIで左テント下小脳半球に腫瘍性病変を認め、毎年MRIにてスクリーニングを行い、今回腫瘍の増大を認め当科紹介となった。神経症状はないが組織診断目的で腫瘍摘出術を行った。腫瘍は正常小脳実質との境界は明瞭で、白色の弾性軟で血管成分が乏しく低悪性度神経膠腫が疑われた。術野にメラニン色素増殖部位は認めず、小脳歯状核機能温存のため近傍の腫瘍を残して終了した。病理診断はび漫性星細胞腫であった。術後に新たな神経症状出現は認めず、創部治癒後に退院した。3ヵ月後、左側歯状核近傍に残存する腫瘍の再摘出術を行った。術後に体幹失調・左側失測定症状の出現を認めたが、リハビリテーション後に独歩可能状態で退院した。
  • 中山 若樹, 石川 達哉, 宝金 清博, 黒田 敏, 上山 博康 脳神経外科ジャーナル 20 (0) 46 -46 2011年04月10日 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博 Medical Practice 28 (4) 681 -684 2011年04月 [査読無し][通常論文]
  • 宮田 圭, 栗本 義彦, 浅井 康文, 飯星 智史, 小柳 泉, 三國 信啓, 宝金 清博 脳神経外科速報 21 (4) 430 -435 2011年04月 [査読無し][通常論文]
     
    50歳男。車にひかれ受傷し救急搬送された。来院時はGCS 7、ショック状態で、CTでびまん性脳損傷、横突孔骨折を伴う軸椎歯状突起骨折、胸部下行大動脈損傷、不安定型骨盤骨折、両大腿骨開放骨折等を合併する重症多発外傷の所見を認めた。腹部大動脈損傷に対しステントグラフト内挿術を施行し、骨盤骨折に対し観血的骨盤固定術を施行した。大動脈損傷部位は左鎖骨下動脈分岐直後であったため、ステントを左総頸動脈分岐直後から留置し、意図的に左鎖骨下動脈を閉鎖した。術後の頭頸部血管3D-CTAでは、第2頸椎横突孔貫通部での右椎骨動脈閉塞を認めた。第3病日に従命反応、覚醒を確認し人工呼吸管理を離脱したが、徐々に意識障害が悪化して第8病日にGCS 8となった。第10病日に血管撮影を施行したところ、意識障害の原因として左鎖骨下動脈盗血現象、椎骨脳底動脈血流不全が疑われ、第12病日に左鎖骨下動脈の血行再建目的に腋窩動脈-腋窩動脈バイパス術を施行した。術後意識は清明となり、第23病日に転医となった。
  • 鈴木 則宏, 佐藤 志樹, 内山 真一郎, 岡田 靖, 折笠 秀樹, 後藤 信哉, 重松 宏, 代田 浩之, 田中 耕太郎, 寺山 靖夫, 宝金 清博, 松本 昌泰, 峰松 一夫, 宮本 享, EVEREST学術委員会 分子脳血管病 10 (2) 163 -167 2011年04月 [査読無し][通常論文]
     
    EVEREST(Effective Vascular Event Reduction after Stroke)は、最終発症後2週間〜6ヵ月の外来脳梗塞患者を対象とした前向きの観察研究である。本研究を実施した結果、脳梗塞の1年再発率は3.8%(95%信頼区間3.2〜4.5%)で、退院後も脳梗塞再発のリスクが高いことが確認された。今後は、得られたデータから脳梗塞再発のリスク因子を同定する予定である。(著者抄録)
  • 笹森 徹, 飛騨 一利, 浅野 剛, 青山 剛, 山内 朋裕, 岩崎 素之, 矢野 俊介, 岩崎 喜信, 宝金 清博 脊髄外科 25 (1) 81 -83 2011年04月 [査読無し][通常論文]
  • 青山 剛, 飛騨 一利, 浅野 剛, 矢野 俊介, 宝金 清博 Journal of Spine Research 2 (3) 628 -628 2011年03月 [査読無し][通常論文]
  • Toshiya Sugino, Takeshi Mikami, Satoshi Iihoshi, Kiyohiro Houkin STROKE 42 (3) E344 -E344 2011年03月 [査読無し][通常論文]
  • Masaki Ito, Satoshi Kuroda, Taku Sugiyama, Katsuhiko Maruichi, Masahito Kawabori, Hideo Shichinohe, Kiyohiro Houkin STROKE 42 (3) E94 -E95 2011年03月 [査読無し][通常論文]
  • 脊髄難治性腫瘍の治療戦略 再発あるいは悪性髄内腫瘍に対する治療戦略
    飛騨 一利, 浅野 剛, 青山 剛, 宝金 清博 日本整形外科学会雑誌 85 (2) S11 -S11 2011年02月 [査読無し][通常論文]
  • 眼窩先端部症候群で発症し内頸動脈浸潤した副鼻腔真菌症の3症例
    杉山 拓, 黒田 敏, 中山 若樹, 寳金 清博 Neurological Surgery 39 (2) 155 -161 2011年02月 [査読有り][通常論文]
     
    症例1:83歳男。肝細胞癌、糖尿病の既往があり、抗悪性腫瘍薬を内服中であった。左眼周囲痛、複視、視力低下が出現し、頭部MRIで副鼻腔から眼窩、側頭筋、海綿静脈洞部にかけて広範な浸潤性病変を認め、切開排膿・上顎洞開放術を行い、組織中にアスペルギルスが同定された。同日右麻痺、失語が出現し、MRIで左中大脳動脈領域に脳梗塞を認め、点滴治療で改善せず転院となった。症例2:72歳女。再生不良性貧血にてステロイド投与中であった。左上顎歯の抜歯後に潰瘍形成、発熱があり、β-Dグルカンの上昇を認め抗真菌薬が投与された。1ヵ月後より左顔面のしびれ・痛み、左視力低下が出現し、更に右麻痺も生じ、MRIで左中大脳動脈領域に低吸収域を、脳血管撮影で左内頸動脈に狭窄病変を認めた。急性期治療を行ったが、症状および画像所見の悪化を認め、麻痺出現後14日目に死亡した。症例3:62歳男。右上顎洞炎にて開放術を受け、アスペルギルス症の診断で抗真菌薬を投与中であった。徐々に右眼窩周囲痛、眼球突出、複視が出現し、眼窩内容物摘出、ステロイドパルス療法を受けたが、1ヵ月後に右眼窩内より動脈性出血が生じ、脳血管撮影で右内頸動脈に動脈瘤を認めた。トラッピングおよび動脈吻合術を施行し、出血のコントロールは得られたが、脳梗塞を合併して左麻痺を後遺した。
  • 七戸秀夫, 黒田敏, 工藤與亮, 伊東雅基, 川堀真人, 宮本倫行, 宝金清博, 寺江聡 再生医療 10 257 2011年02月01日 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 20 (2) 123 -128 2011年02月 [査読無し][通常論文]
     
    日本の脳神経外科からの国際的な臨床研究発表は近年増加しておらず, 世界的な評価は相対的に低下している.臨床研究では, ランダム化比較試験が重要である.しかし, 症例研究などの日常診療と直結した臨床研究の重要性を過小評価してはならない.今日, 臨床研究は厳しい社会的な評価の対象であり, 正しい研究方法を習得しなければ, グローバルスタンダードを満たす臨床研究は不可能である.日本の脳神経外科の水準を維持し, さらに国際的な地位を高めるために, 臨床研究に対する教育も含め, 学会全体のレベルアップが必須である.
  • 笹森 徹, 馬渕 正二, 森脇 拓也, 新谷 好正, 浅野 剛, 寶金 清博 脳卒中の外科 = Surgery for cerebral stroke 39 (1) 54 -57 2011年01月31日 [査読無し][通常論文]
  • 伊東 雅基, 黒田 敏, 中山 若樹, 安田 宏, 杉山 拓, 穂刈 正昭, 磯部 正則, 寶金 清博 脳卒中の外科 = Surgery for cerebral stroke 39 (1) 40 -47 2011年01月31日 [査読無し][通常論文]
  • 中山 若樹, 寶金 清博 医学のあゆみ 236 (2) 116 -123 2011年01月08日 [査読無し][通常論文]
  • 金子貞洋, 遠藤将吾, 小林浩之, 浅岡克行, 善積威, 寺坂俊介, 寶金清博 日本脳腫瘍の外科学会プログラム・抄録集 16th 2011年
  • 内野 晴登, 寺坂 俊介, 山口 秀, 小林 浩之, 川合 かがり, 久保田 佳奈子, 大江 真司, 宝金 清博 BRAIN and NERVE: 神経研究の進歩 63 (1) 79 -83 2011年01月 [査読無し][通常論文]
     
    50歳男。4年前に低γグロブリン血症、胸腹部リンパ節病変のMycobactrium genavense(M.genavense)検出より、γグロブリン補充とストレプトマイシン(SM)、クラリスロマイシン(CAM)、リファンピシン(RFP)、レボフロキサシン(LVFX)、エタンブトール(EB)が投与された。今回、頭痛、複視の出現、頭蓋内の多発性腫瘤性病変で入院となった。構音障害、運動性失語、右不全片麻痺、リンパ球とCD4+T細胞の減少、sIL2-Rの軽度上昇を認めた。頭部CTで両側大脳半球の皮髄境界を中心に多発する高吸収で強い脳浮腫を伴う腫瘤病変はT1強調像で高信号、T2強調像は内部不均一な低信号で辺縁は高信号を呈し、拡散強調像で低信号を呈した。腹部CTでリンパ節に腫瘤性病変、FDG-PETで頭部と腹部病変に高集積を認めた。ステロイドと浸透圧利尿薬投与で症状の著明な改善、頭部と腹部病変の縮小を認めたが、確定診断のため開頭生検術を施行した。病理所見よりM.genavenseによる非結核性抗酸菌(NTM)頭蓋内感染と診断し、CAM、RFP、LVFXの3剤投与を開始し6ヵ月後に著明な縮小、症状の消失を認めた。
  • 鈴木 健吾, 三上 毅, 飯星 智史, 馬場 雄大, 黒田 敏, 宝金 清博 脳卒中 33 (1) 31 -36 2011年01月 [査読無し][通常論文]
     
    北海道において新規登録された出血発症もやもや病について検討した。対象は2002年から2009年に登録されたもやもや病416症例のうち、出血発症85例と虚血発症228例を比較した。その結果、1)期間中の出血発症もやもや病の疾患同定率は0.21人/10万人/年であり、男女比は1:1.9で女性優位であった。2)虚血発症のピークが5〜9歳と45〜49年の二峰性を示すのに対し、出血発症のピークは35〜39歳と55〜64年の二峰性を示し、高齢で発症であるのは以前と変わらなかった。3)出血発症群の発症時ADLは虚血発症群と比べて有意に悪く、登録時点での血行再建術施行も、虚血発症25.1%に対して出血発症群19.8%と少ない傾向にあった。4)もやもや病全体の疾患同定率は増加してきており、とりわけ成人における虚血発症例の疾患同定率が増加傾向にあった。
  • 齋藤久泰, 黒田敏, 中山若樹, 吉田大介, 寺江聡, 平田健司, 志賀哲, 玉木長良, 寶金清博 脳循環代謝 23 (1) 115 2011年 [査読無し][通常論文]
  • 飛騨 一利, 浅野 剛, 青山 剛, 宝金 清博 脳神経外科ジャーナル 20 (1) 20 -28 2011年01月 [査読無し][通常論文]
     
    脊髄動静脈奇形ではAV shuntの局在部位から,dural AVF,epidural AVF,perimedullary AVF,そしてintramedullary AVMと分類し,治療方針を立てるのが実用的である.治療としては,dural AVF,epidural AVFについてはNBCAを主体とした塞栓術を第一選択とし,perimedullary AVFについてはType1,Type2では観血的手術を第一選択とし,Type3ではコイルあるいはNBCAを使用した塞栓術を用いている.またintramedullary AVMではPVA顆粒を用いた塞栓術が主であったが,近年サイズの小さなAVMでは定位放射線照射なども試みている.手術においては,術中DSA,indigocarmine動注,術中ICG(indocyanine green)動注による画像診断,さらにMEPの活用など集学的治療が必要であり,放射線科との密接な連携が必要といえる.
  • 金子 貞洋, 寺坂 俊介, 小林 浩之, 宝金 清博, 久保田 佳奈子 脳神経外科ジャーナル 20 (8) 612 -616 2011年 [査読無し][通常論文]
  • もやもや病の治療経過中にバセドウ病と診断された1男児例
    竹内 孝子, 鎌崎 穂高, 要藤 裕孝, 永井 和重, 堤 裕幸, 三上 毅, 宝金 清博 日本小児科学会雑誌 114 (12) 1935 -1935 2010年12月 [査読無し][通常論文]
  • 齊藤 正樹, 米増 保之, 高橋 明, 吉田 英人, 大森 義範, 岡田 靖, 矢坂 正弘, 下濱 俊, 山本 和利, 寳金 清博 脳卒中 32 (6) 684 -688 2010年11月25日 [査読無し][通常論文]
  • 齊藤 正樹, 米増 保之, 山本 和利, 姉川 敬裕, 崎間 邦洋, 高橋 明, 矢坂 正弘, 岡田 靖, 下濱 俊, 寳金 清博 脳卒中 32 (6) 689 -693 2010年11月25日 [査読無し][通常論文]
  • 浅野 剛, 飛騨 一利, 柏崎 大奈, 青山 剛, 宝金 清博, 矢野 俊介 JNET: Journal of Neuroendovascular Therapy 4 (4) 208 -208 2010年11月 [査読無し][通常論文]
  • 血清を用いずに培養したヒト骨髄間質細胞のラット脳梗塞モデルへの移植およびMRIによる移植細胞追跡
    伊東 雅基, 黒田 敏, 杉山 拓, 七戸 秀夫, 武田 紫, 西尾 充史, 小池 隆雄, 寶金 清博 脳循環代謝 22 (1) 128 -128 2010年11月 [査読無し][通常論文]
  • 川堀 真人, 黒田 敏, 七戸 秀夫, 宝金 清博 再生医療 9 (4) 477 -486 2010年11月 [査読無し][通常論文]
     
    近年の膨大な研究成果をもとに、さまざまな細胞ソースを用いた中枢神経の再生医療が臨床応用の段階を迎えようとしている。その主なターゲットは脳梗塞、脳挫傷、脊髄損傷、神経変性疾患などである。しかし、その移植細胞投与方法に関しては現時点においても定位的移植、経静脈的移植などさまざまな移植方法が個々に検討されていることがほとんどで、最適な移植ルートに関して科学的に比較検証されていないのが実情である。近い将来に展開されると考えられる臨床応用を考慮した場合、中枢神経への安全かつ効率的な移植ルートを科学的データに基づいて確立しておくことが急務である。本稿では、現在までに動物あるいはヒトで実施されてきた幹細胞の中枢神経への細胞移植の成果を、脳梗塞を中心に概説し、安全かつ効率的な移植ルートについて検証する。(著者抄録)
  • 七戸 秀夫, 黒田 敏, 杉山 拓, 伊東 雅基, 川堀 真人, 西尾 充史, 武田 紫, 小池 隆夫, 寳金 清博 脳循環代謝 22 (1) 128 -128 2010年11月 [査読無し][通常論文]
  • 黒田 敏, 七戸 秀夫, 杉山 拓, 伊東 雅基, 川堀 真人, 千葉 泰弘, 長内 俊也, 丸一 勝彦, 宝金 清博, 岩崎 喜信 脳卒中 32 (6) 532 -537 2010年11月 [査読無し][通常論文]
     
    数多くの基礎研究によって、骨髄間質細胞(BMSC)移植は脳梗塞や脊髄損傷後の神経症状の回復を有意に促進することが証明されている。基礎研究の成果を基に、中枢神経疾患に対するBMSC移植が国内外で小規模な臨床試験として開始されつつある。脳梗塞に対してBMSC移植を臨床応用するに当って解決すべき以下の問題点と解決策について述べた。1)ヒトBMSCの培養法、2)ヒトBMSC移植のプロトコール、3)ヒトにおける細胞イメージング。
  • Izumi Koyanagi, Kiyohiro Houkin NEUROSURGICAL REVIEW 33 (4) 506 -506 2010年10月 [査読無し][通常論文]
  • 宝金 清博, 三上 毅 Medical Torch 6 (2) 28 -31 2010年10月 [査読無し][通常論文]
  • 宝金 清博, 花北 順哉 脳神経外科速報 20 (9) 988 -997 2010年09月 [査読無し][通常論文]
  • 宝金 清博, 白根 礼造 小児の脳神経 35 (4) 395 -395 2010年08月31日 [査読無し][通常論文]
  • ヒト骨髄間質細胞の安全で効率的な培養法 骨髄間質細胞移植による中枢神経再生の臨床応用にむけて
    伊東 雅基, 黒田 敏, 杉山 拓, 七戸 秀夫, 武田 紫, 西尾 充史, 小池 隆夫, 寶金 清博 再生医療 9 (3) 385 -392 2010年08月 [査読無し][通常論文]
     
    近年、脳梗塞や脊髄損傷などの不可逆的中枢神経損傷に対して、多分化能を有する骨髄間質細胞(bone marrow stromal cell:BMSC)を移植することで失われた神経機能を回復させる試みが数多くの研究者により報告されている。BMSCは自己骨髄から比較的容易に採取可能で、最も早く臨床応用が期待されている体性幹細胞の1つである。今後の幅広い臨床応用を考慮した場合、安全かつ効率的に必要なBMSCを供給可能な培養法の確立が急務である。BMSCの特性を失うことなく安全かつ効率的に培養するための研究について、中枢神経再生分野での現状、最近の新しい知見について概説する。(著者抄録)
  • 眞島 任史, 小山 司, 寶金 清博, 遠山 晴一, 中川 伸 北海道醫學雜誌 = Acta medica Hokkaidonensia 85 (3) 157 -160 2010年05月01日 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 19 (0) 60 -60 2010年04月20日 [査読無し][通常論文]
  • 越智 さと子, 吉藤 和久, 南田 善弘, 小柳 泉, 宝金 清博 小児の脳神経 35 (2) 206 -206 2010年04月 [査読無し][通常論文]
  • 小柳 泉, 村上 友宏, 金子 高久, 吉藤 和久, 宝金 清博 小児の脳神経 35 (2) 268 -268 2010年04月 [査読無し][通常論文]
  • Diffuse brain injury(びまん性脳損傷)の急性期加療
    宮田 圭, 小柳 泉, 宝金 清博, 浅井 康文 日本脳神経外傷学会プログラム・抄録集 33回 79 -79 2010年03月 [査読無し][通常論文]
  • 強直性脊椎炎に伴う頸椎骨折の1例
    金子 高久, 小柳 泉, 村上 友宏, 宝金 清博 日本脳神経外傷学会プログラム・抄録集 33回 96 -96 2010年03月 [査読無し][通常論文]
  • 小柳 泉, 村上 友宏, 金子 高久, 吉藤 和久, 宝金 清博 脳神経外科ジャーナル 19 (1) 32 -40 2010年01月 [査読無し][通常論文]
     
    頚椎変性疾患に対する外科治療選択,特に前方あるいは後方アプローチの選択は従来議論の多いところである.われわれは,1〜2椎間圧迫病変に対しては主に前方固定,3椎間以上の多椎間病変や黄色靭帯による圧迫が主体の症例には後方除圧(両開き式椎弓形成術)を行ってきた.今回,1996〜2007年に著者が外科治療を行った頚椎症263例,頚椎OPLL91例について,おのおのの術式での合併症や問題点を分析した.文献のレビューと合わせ,術式選択に関して考察した.前方固定は227例,後方除圧は127例であった.術後に新たに発生した上肢症状は前方固定群で少なく,経過観察中の再手術は後方除圧群で少ない結果であった.後方除圧群では平均で11度の頚椎の可動制限がみられた.前方および後方除圧にはおのおの十分理解しておくべき利点と欠点がある.外科治療の選択は,圧迫レベルの矢状方向の伸展,後方からの圧迫要因,頚椎の後弯変形,全身状態などをよく考慮して個々の症例で決定するべきであろう.
  • 飯星 智史, 原田 邦明, 宮田 圭, 野中 雅, 小柳 泉, 寶金 清博 CI研究 : progress in computed imaging 31 (3) 171 -178 2009年12月31日 [査読無し][通常論文]
  • 本望 修, 大石 美里, 宝金 清博 東北脳血管障害研究会学術集会記録集 31回 73 -78 2009年12月 [査読無し][通常論文]
  • 齊藤 正樹, 山本 和利, 米増 保之, 松村 晃寛, 豊島 貴信, 崎間 洋邦, 前田 亘一郎, 中村 麻子, 橋本 治, 蜂須賀 明子, 高橋 明, 矢坂 正弘, 岡田 靖, 長峯 隆, 今井 富裕, 下濱 俊, 佐藤 利夫, 寳金 清博, CE委員会 臨床神経学 49 (12) 1182 -1182 2009年12月 [査読無し][通常論文]
  • 齊藤 正樹, 奈良 理, 大里 俊明, 宮田 圭, 浅井 康文, 中川原 譲二, 寳金 清博, 端 和夫 脳卒中 31 (6) 586 -589 2009年11月25日 [査読無し][通常論文]
  • 宮田 圭, 飯星 智史, 宝金 清博, 野中 雅 JNET: Journal of Neuroendovascular Therapy 3 (4) 368 -368 2009年11月 [査読無し][通常論文]
  • K. Houkin, O. Honmou, T. Matsunaga, S. Ishiai, S. Waxman, J. Kocsis JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM 29 S560 -S560 2009年10月 [査読無し][通常論文]
  • 吉藤 和久, 小柳 泉, 越智 さと子, 宝金 清博 小児の脳神経 34 (5) 374 -379 2009年10月 [査読無し][通常論文]
     
    小児の腰仙部椎弓正中部は未骨化なため、正常でもCT上欠損してみえることの特徴について以前報告した。今回は病的椎弓欠損(二分脊椎)の特徴について、脊髄円錐部脂肪腫と終糸脂肪腫を基にまとめて報告する。終糸脂肪腫では、仙骨下部から尾骨にかけ軽度の椎弓非対称および脊柱配列異常を認めるものの、椎弓正中部の欠損所見は正常例と同様と考えられた。円錐部脂肪腫では椎弓欠損数と最大欠損幅が終糸脂肪腫より有意に大きく、かつ頭側へ及び、また椎弓の後方へ「扉が開くような」変形、椎体形成不全、異所性骨化を特徴として認めた。(著者抄録)
  • 越智 さと子, 吉藤 和久, 小柳 泉, 宝金 清博 小児の脳神経 34 (5) 385 -390 2009年10月 [査読無し][通常論文]
     
    新生児期に治療したterminal myelocystoceleの2例を報告する。1例は左側に限局した下半身の内臓奇形、鎖肛と左下肢低形成を伴う男児で、手術所見上、左側で円錐は脂肪腫と癒着し左馬尾神経は低形成だった。胎生期器官形成に対する。脊髄奇形の影響を考える上で示唆的だった。内臓奇形を伴わない1例と併せ、画像上の特徴や発生機序について考察した。2例とも神経因性膀胱にて導尿に移行したが、装具を用い自力で歩行可能になった。QOLを考える上で、早期手術と関係科との協力、連携が重要だった。(著者抄録)
  • 本望 修, 宝金 清博 医学のあゆみ 231 (5) 553 -556 2009年10月 [査読無し][通常論文]
     
    脳梗塞亜急性期の患者に対して自己の骨髄幹細胞を静脈内に投与することで、神経症状の改善が期待されている。1990年代初期から、著者らは神経移植・再生治療の確立をめざして、神経系細胞をはじめとする種々のドナー細胞を用いた細胞移植研究を開始し、とくに神経幹細胞やES細胞などの幹細胞を用いた基礎研究を展開してきた。近年では実用化を念頭に、臨床応用に最短位置と予想される骨髄細胞をドナー細胞とした神経再生研究に注目し、そのなかでもとくに神経再生作用の強い細胞群(骨髄間葉系幹細胞)が、経静脈内投与でも実験的脳梗塞に対して著明な治療効果が認められることを報告してきた。これらの基礎研究結果に基づき、2007年1月より脳梗塞亜急性期の患者を対象とした自己骨髄間葉系幹細胞の静脈内投与について、その安全性と治療効果の検討を行っている。(著者抄録)
  • 本望 修, 宝金 清博 Surgery Frontier 16 (3) 315,265 -266 2009年09月 [査読無し][通常論文]
     
    脳梗塞の亜急性期の患者に対して、自己の骨髄幹細胞を静脈内に投与することで、神経症状の改善が期待されている。1990年代初期からわれわれは、神経移植・再生治療の確立を目指して、神経系細胞をはじめとする種々のドナー細胞を用いた細胞移植研究を開始し、特に神経幹細胞やES細胞などの幹細胞を用いた基礎研究を展開してきた。近年では実用化を念頭に臨床応用に最短位置と予想される骨髄細胞をドナー細胞とした神経再生研究に注目し、その中でも特に神経再生作用の強い細胞群(骨髄間葉系幹細胞)が、経静脈内投与でも実験的脳梗塞に対して著明な治療効果が認められることを報告してきた。これらの基礎研究結果に基づき、2007年1月より脳梗塞亜急性期の患者を対象とした自己骨髄間葉系幹細胞の静脈内投与について、その安全性と治療効果について検討している。(著者抄録)
  • 本望 修, 宝金 清博 Progress in Medicine 29 (9) 2209 -2213 2009年09月 [査読無し][通常論文]
  • 黒田 敏, 川堀 真人, 宮本 倫行, 笹森 徹, 遠藤 将吾, 中山 若樹, 石川 達哉, 宝金 清博, 岩崎 喜信 脳卒中の外科 37 (5) 345 -349 2009年09月 [査読無し][通常論文]
     
    1998年4月〜2008年7月にもやもや病と診断され、脳血行再建術を施行された71例117側中同側後大脳動脈に高度の狭窄を有し、側頭葉から後頭葉にかけての神経症状と著しい脳虚血を有していた4症例(全例女性、8〜38歳)に対する術式の実際を呈示した。その結果、術前MRIでは2例には脳梗塞を認めず、他の2例では後頭葉から側頭葉下面にかけて脳梗塞を認め、PETでは前頭葉の虚血に加え側頭葉から後頭葉の著しい脳虚血を認めた。術後3〜4ヵ月の外頸動脈撮影ではSTA-MCA吻合部と間接バイパス術を介して前頭部のみならず側頭部から後頭部にかけて側副血行路が広範に形成されていた。以上より、後大脳動脈狭窄病変のため前頭葉、側頭葉や後頭葉に高度の脳虚血を有し、その領域に起因する脳虚血発作を呈する症例では、一期的に実施する後方に拡大した複合的血行再建術が周術期の脳虚血合併症を予防し、脳虚血発作を消失させる上で有用と考えられた。
  • 当施設における頭部外傷後遺症患者の追跡調査
    宮田 圭, 奈良 理, 森 和久, 浅井 康文, 小柳 泉, 宝金 清博 日本救急医学会雑誌 20 (8) 631 -631 2009年08月 [査読無し][通常論文]
  • 本望 修, 寶金 清博 成人病と生活習慣病 39 (8) 929 -932 2009年08月 [査読無し][通常論文]
  • 本望 修, 大石 美里, 宝金 清博 脳21 12 (3) 318 -321 2009年07月 [査読無し][通常論文]
     
    脳梗塞に対する治療は、従来も今後も急性期治療が主体となることは間違いがない事実であるが、近年、脳梗塞の亜急性期においても、自己の骨髄幹細胞を静脈内に投与することで、神経症状の改善が期待できる可能性が示唆されてきている。体性幹細胞を用いた再生医療は骨髄移植などを代表されるが、成人の生体内にある幹細胞を使用する細胞治療は既に一部は実用化されているとおり、安全性が高く、自己移植が可能などの利点があるため、早急な実用化が期待されている。特に、骨髄幹細胞は、非常に少量の骨髄液から培養・増殖させることが可能であり、臨床応用においては誠に使い勝手の良い細胞として認知されつつある。骨髄幹細胞の治療メカニズムは、大きく分けて、(1)サイトカインによる神経栄養・保護作用、(2)血管新生作用、(3)神経再生、の3つがあるが、これらの多彩な作用メカニズムを考慮すると、脳梗塞以外の脳卒中への応用も期待される。(著者抄録)
  • 脳梗塞に対する骨髄幹細胞治療
    宝金 清博 Pharma Medica 27 (Suppl.1) 17 -23 2009年07月 [査読無し][通常論文]
  • 【脳神経外科 最新ギアの最前線】外科医と道具 ローテクとハイテク
    宝金 清博 Medical Torch 5 (1) 20 -21 2009年06月 [査読無し][通常論文]
     
    外科医は素手では手術しない。道具を介して治療する。これは言うまでもないことである。しかし、外科医の手が道具を介して、いわば直線的に患者に操作を加える単純な関係性がデジタル技術の導入により大きく変貌してきている。「手」の動作が比較的直接的に伝わってきたローテクの伝統的な分野とデジタル技術を介するハイテクとが、私たち外科医の世界を大きく変えつつある。この2つの技術の新しい融合が進行中の時代に私たちは生きている。少し立ち止まって、外科医のローテクとデジタルハイテクの関係性を真剣に考えてみることも必要である。それは、外科治療のあり方そのものに関わる本質的な問題を内包している。(著者抄録)
  • 加藤 天美, 鎌田 恭輔, 宝金 清博, 森田 明夫 Medical Torch 5 (1) 8 -19 2009年06月 [査読無し][通常論文]
  • 原口 浩一, 外山 賢太郎, 加藤 孝顕, 真鍋 宏, 坂本 靖男, 蓮沼 正博, 伊藤 丈雄, 野中 唯, 寶金 清博 脳卒中の外科 = Surgery for cerebral stroke 37 (3) 179 -183 2009年05月31日 [査読無し][通常論文]
     
    We report 6 cases with symptomatic intracranial vertebral artery stenosis treated with percutaneous transluminal angioplasty (PTA).
    Five of 6 cases had no contralateral vertebral artery (VA) and only 1 had hypoplastic contralateral VA. PTA was urgently performed in 3 cases presenting with progressive infarction. We used a balloon-expandable coronary stent against vertebral artery dissection subsequently after PTA in 1 case. No complications occurred during the procedure in any of the cases. Five cases were discharged from our hospital and could live independently. In 1 case in which PTA was urgently performed, disturbance of consciousness and tetraparesis remained.
    The presence of a high-grade vertebral artery stenosis and a contralateral vertebral artery hypoplasia or occlusion can induce a progressive infarction in the early stage, so immediate revascularization should be considered in these cases.
  • 本望 修, 宝金 清博 神経治療学 26 (3) 294 -294 2009年05月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 18 (0) 74 -74 2009年04月20日 [査読無し][通常論文]
  • 本望 修, 大石 美里, 宝金 清博 分子脳血管病 8 (2) 169 -172 2009年04月 [査読無し][通常論文]
     
    脳梗塞の亜急性期の患者に対して、自己の骨髄幹細胞を静脈内に投与することで、神経症状の改善が期待される。幹細胞の治療メカニズムを考えると、脳梗塞以外の脳神経疾患への適応拡大の可能性が期待され、今後、更なる研究が望まれる。(著者抄録)
  • 吉藤 和久, 越智 さと子, 村上 友宏, 小柳 泉, 宝金 清博 小児の脳神経 34 (2) 187 -187 2009年04月 [査読無し][通常論文]
  • 小柳 泉, 吉藤 和久, 村上 友宏, 越智 さと子, 宝金 清博 小児の脳神経 34 (2) 223 -223 2009年04月 [査読無し][通常論文]
  • 村上 友宏, 小柳 泉, 宝金 清博 小児の脳神経 34 (2) 224 -224 2009年04月 [査読無し][通常論文]
  • 頭・頸部杙創の一例
    宮田 圭, 秋山 幸功, 宝金 清博, 浅井 康文 日本神経外傷学会プログラム・抄録集 32回 102 -102 2009年04月 [査読無し][通常論文]
  • Takeo Abumiya, Kiyohiro Houkin, Satoshi Morita, Shunichi Fukuhara STROKE 40 (4) E248 -E248 2009年04月 [査読無し][通常論文]
  • 鐙谷 武雄, 宝金 清博 日本血栓止血学会誌 20 (2) 212 -212 2009年04月 [査読無し][通常論文]
  • 本望 修, 宝金 清博 綜合臨床 58 (2) 217 -220 2009年02月 [査読無し][通常論文]
     
    われわれは、神経系細胞を始めとする種々のドナー細胞の研究を1990年代初頭から開始し、そのなかでもとくに、神経幹細胞やES細胞などの幹細胞を用いた細胞移植療法に関する基礎研究を行ってきた。1990年代半ばからは、実用化を念頭に、臨床応用に最短位置と思われた骨髄細胞をドナー細胞とした神経再生研究に着目し、基礎研究成果を数多く報告してきた。このような一連の細胞移植研究の結果、骨髄の中に、とくに神経再生作用の強い細胞群(骨髄間葉系幹細胞)が存在し、しかも、経静脈内投与でも実験的脳梗塞に対して著明な治療効果が認められることを報告してきた。これらの基礎研究結果に基づき、平成19年1月より脳梗塞亜急性期の患者を対象とした自己骨髄間葉系幹細胞の静脈内投与について、その安全性と治療効果について検討しており、従来の脳梗塞治療による経過と比較して、きわめて回復が良いと思われる症例も散見され始めている。幹細胞による治療効果は、ドナー細胞とホスト側の反応の総和であり、多彩な作用メカニズムが時間的にも空間的にも多段階的に作用する。また治癒過程も、従来の自然経過とはまったく異なる表現系となる。したがって、リハビリテーションの方法や目標設定もまったく異なる。(著者抄録)
  • 小児頭部外傷患者の転帰 身体・精神後遺障害の追跡調査
    宮田 圭, 奈良 理, 浅井 康文, 小柳 泉, 宝金 清博 日本脳神経外科救急学会プログラム・抄録集 14回 64 -64 2009年01月 [査読無し][通常論文]
  • Masanori Sasaki, Christine Radtke, Osamu Honmou, Kiyohiro Houkin, Jeffery Kocsis NEUROSCIENCE RESEARCH 65 S127 -S127 2009年 [査読無し][通常論文]
  • 飯星 智史, 寶金 清博 からだの科学 (260) 21 -26 2009年 [査読無し][通常論文]
  • 寶金 清博 日本病院会雑誌 = Journal of Japan Hospital Association 56 (1) 67 -71 2009年01月01日 [査読無し][通常論文]
  • 佐古 和廣, 郷 一知, 寶金 清博, 粟井 是臣, 安藤 恒三郎, 小川, 中村 日本病院会雑誌 = Journal of Japan Hospital Association 56 (1) 77 -83 2009年01月01日 [査読無し][通常論文]
  • 本望 修, 大石 美里, 宝金 清博 東北脳血管障害研究会学術集会記録集 31 (0) 73 -78 2008年12月06日 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (12) 1484 -1488 2008年12月 [査読無し][通常論文]
  • 本望 修, 宝金 清博 Medical Practice 25 (12) 2237 -2238 2008年12月 [査読無し][通常論文]
  • 神経疾患治療ノート もやもや病
    飯星 智史, 宝金 清博 Clinical Neuroscience 26 (12) 1399 -1401 2008年12月 [査読無し][通常論文]
  • 科学的根拠に基づくくも膜下出血診療ガイドライン第2版
    吉峰 俊樹, 伊達 勲, 橋本 信夫, 本郷 一博, 宝金 清博, 河瀬 斌, 嘉山 孝正, 桐野 高明, 木内 博之, 甲村 英二, 宮本 享, 永田 泉, 小川 彰, 佐伯 直勝, 斉藤 勇, 斉藤 延人, 坂井 信幸, 佐野 公俊, 佐々木 富男, 塩川 芳昭, 高橋 明, 滝 和郎, 種子田 護, 冨永 悌二, 上島 弘嗣, 山田 和雄, 山口 武典, 山浦 晶, 吉田 純, 藤中 俊之, 中村 元, 江面 正幸, 藤村 幹, 藤田 敦史, 古屋 一英, 服部 健一, 飯原 弘二, 井上 敬, 柿澤 幸成, 樫村 博史, 片岡 和夫, 北川 直毅, 小林 英一, 近藤 礼, 栗田 浩樹, 間瀬 光人, 宮地 茂, 森田 明夫, 詠田 眞治, 野中 雅, 野崎 和彦, 大坪 亮一, 岡本 奨, 岡村 智教, 小野 成紀, 小野塚 聡, 阪井田 博司, 杉田 正夫, 高木 康志, 梅村 淳, 清水 宏明, 日本脳卒中の外科学会, くも膜下出血診療ガイドライン改訂委員会 脳卒中の外科 36 (増刊) 1 -80 2008年12月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (11) 1352 -1357 2008年11月 [査読無し][通常論文]
  • 野中 雅, 飯星 智史, 宮田 圭, 斉藤 正樹, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 2 (4) 163 -163 2008年11月 [査読無し][通常論文]
  • 宮田 圭, 飯星 智史, 斉藤 正樹, 野中 雅, 小柳 泉, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 2 (4) 209 -209 2008年11月 [査読無し][通常論文]
  • 原口 浩一, 加藤 孝顕, 真鍋 宏, 坂本 靖男, 蓮沼 正博, 伊藤 丈雄, 宝金 清博, 野中 雅, 外山 賢太郎 JNET: Journal of Neuroendovascular Therapy 2 (4) 225 -225 2008年11月 [査読無し][通常論文]
  • 飯星 智史, 宮田 圭, 斉藤 正樹, 野中 雅, 小柳 泉, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 2 (4) 229 -229 2008年11月 [査読無し][通常論文]
  • 齊藤 正樹, 飯星 智史, 宮田 圭, 米増 保之, 高橋 明, 金 相年, 大瀧 雅文, 大坊 雅彦, 宝金 清博, 野中 雅 JNET: Journal of Neuroendovascular Therapy 2 (4) 237 -237 2008年11月 [査読無し][通常論文]
  • 脳虚血における脳保護・再生 どこまでできるか 脳梗塞における臨床応用
    本望 修, 宝金 清博 脳循環代謝 20 (1) 75 -75 2008年11月 [査読無し][通常論文]
  • 心肺停止後全脳虚血に対する骨髄幹細胞移植の検討
    鄭 偉, 本望 修, 劉 赫, 原田 邦明, 鈴木 淳平, 宝金 清博 脳循環代謝 20 (1) 99 -99 2008年11月 [査読無し][通常論文]
  • MRI-FAIR法とSPECT法 脳血流評価についての検討
    飯星 智史, 原田 邦明, 小倉 圭史, 木村 友亮, 野中 雅, 小柳 泉, 宝金 清博 脳循環代謝 20 (1) 141 -141 2008年11月 [査読無し][通常論文]
  • インドシアニングリーン(ICG)による術中脳血管造影の有用性について
    木村 友亮, 飯星 智史, 宝金 清博 脳循環代謝 20 (1) 155 -155 2008年11月 [査読無し][通常論文]
  • 医療におけるアクシデント・インシデント発生のリアルタイム把握・分析法の開発
    明石 浩史, 中橋 望, 高塚 伸太朗, 篠村 恭久, 宝金 清博 医療情報学連合大会論文集 28回 1134 -1135 2008年11月 [査読無し][通常論文]
  • 飯星 智史, 宝金 清博 分子脳血管病 7 (4) 457 -461 2008年10月 [査読無し][通常論文]
     
    脳卒中は全世界の患者における主要な死因として、また後遺障害をもたらす重要な疾患として認知され、脳神経外科医、神経内科医を大いに悩ます。この半世紀、脳神経外科医は虚血脳への血行再建を安全に、より有効な方法で模索してきた。最初の頭蓋外-頭蓋内血行再建術から最近の技術的進化が著しい血管内治療まで、脳の血行再建技術は、常に進歩している。本論文では、脳血行再建に関する治療の歴史的進歩を考察する。(著者抄録)
  • 宝金 清博 分子脳血管病 7 (4) 494 -495 2008年10月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (10) 1226 -1230 2008年10月 [査読無し][通常論文]
  • 脳血管障害治療における血圧管理
    宝金 清博 日本高血圧学会総会プログラム・抄録集 31回 130 -130 2008年10月 [査読無し][通常論文]
  • Chiari 1型奇形症例における脳幹の下垂と病態についての一考察
    村上 友宏, 小柳 泉, 大蔵 篤彦, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 67回 2J -04 2008年10月 [査読無し][通常論文]
  • 前方進入頸椎手術中の頸椎配列
    大蔵 篤彦, 小柳 泉, 村上 友宏, 吉藤 和久, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 67回 2J -07 2008年10月 [査読無し][通常論文]
  • 頸椎変性疾患に対する椎弓スペーサーを使用しない両開き椎弓形成術
    小柳 泉, 村上 友宏, 吉藤 和久, 大蔵 篤彦, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 67回 1F -03 2008年10月 [査読無し][通常論文]
  • 自己骨髄間葉系幹細胞の静脈内投与による脳梗塞治療へ向けて
    本望 修, 宝金 清博, 松永 卓也 日本脳神経外科学会総会CD-ROM抄録集 67回 2F -06 2008年10月 [査読無し][通常論文]
  • AngioGuard XPを用いた頸動脈ステント留置術初期成績とその問題点の検討
    野中 雅, 飯星 智史, 宮田 圭, 斉藤 正樹, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 67回 2I -01 2008年10月 [査読無し][通常論文]
  • 医療安全・質向上を目的とした病院横断的多職種ピアレビューとその効果 脳神経外科における合併症率と訴訟率
    宝金 清博, 馬場 雄大, 小柳 泉, 野中 雅, 南田 善弘 日本脳神経外科学会総会CD-ROM抄録集 67回 1A -04 2008年10月 [査読無し][通常論文]
  • Tamas Doczi, Kiyohiro Houkin NEUROSURGICAL REVIEW 31 (4) 395 -396 2008年10月 [査読無し][通常論文]
  • 小林 徹, 黒田 敏, 石川 達哉, 中山 若樹, 宝金 清博, 岩崎 喜信 日本脳神経外科学会総会CD-ROM抄録集 67回 2E -03 2008年10月 [査読無し][通常論文]
  • 黒田 敏, 中山 若樹, 石川 達哉, 宝金 清博, 岩崎 喜信 日本脳神経外科学会総会CD-ROM抄録集 67回 2E -02 2008年10月 [査読無し][通常論文]
  • 寳金 清博, 齊藤 正樹 ベストナース 19 (10) 46 -49 2008年10月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (9) 1105 -1111 2008年09月 [査読無し][通常論文]
  • 本望 修, 宝金 清博 神経治療学 25 (5) 545 -548 2008年09月 [査読無し][通常論文]
  • 寳金 清博, 齊藤 正樹 ベストナース 19 (9) 42 -44 2008年09月 [査読無し][通常論文]
  • 黒田 敏, 中山 若樹, 石川 達哉, 宝金 清博, 上山 博康, 岩崎 喜信 脳卒中の外科 36 (5) 361 -366 2008年09月 [査読無し][通常論文]
     
    脳血行再建術を施行したもやもや病164例262側の成績を報告した。術式はencephalo-myo-synangiosis(EMS)あるいはSTA-MCA anastomosis(SMA)+EMSを23例44側(第1期)に、SMA+encephalo-duro-arterio-myo-synangiosisを83例136側(第2期)に、SMA+encephalo-duro-arterio-myo-arterio-pericranial synangiosisを58例82側(第2期)に施行した。神経症状を後遺した周術期合併症は10例に認め、第1期6.8%、第2期2.9%、第3期3.6%であった。術後の脳血管撮影で、術式にかかわらず直接的バイパス術では小児例で約60%、成人例で90%開存し、間接的バイパス術では小児例では95%以上で形成されていたが、成人例では60%でのみ形成を認めた。術後の側副血行路の形成範囲は開頭範囲に依存していた。SPECTでは第1期で前頭葉における脳血流量や脳血管反応性の低下が遷延していたが、第2期では改善が明らかで、第3期ではPETでさらに脳血液量、脳酸素抽出率の改善が顕著に認められた。長期経過観察では10例に脳血管イベントが生じ、脳梗塞3例、頭蓋内出血7例で、脳梗塞はいずれも新たに発生した狭窄病変によるものであった。
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (8) 968 -972 2008年08月 [査読無し][通常論文]
  • 【脳梗塞の病態と治療の最前線】脳梗塞外科療法の現状と未来
    飯星 智史, 宝金 清博 Medical Science Digest 34 (9) 408 -411 2008年08月 [査読無し][通常論文]
     
    近年の脳血管内外科治療技術の進歩は低侵襲であることもあり、脳卒中の外科治療に大きな変化をもたらした。脳血行再建術においても、頸動脈を含めた頭蓋外の動脈狭窄病変に対する治療では、その多くが血管内外科治療に置き換わりつつある。また、動脈瘤に対する血管内治療の適応も急速に広がり、この結果、外科治療の対象となる脳動脈瘤は、単純な治療法で可能な動脈瘤ではなく、バイパスも含めた複雑な治療を必要とするものが相対的に増えている。また、慢性脳虚血に対するバイパスのエビデンスが確立しつつあり、頭蓋外-頭蓋内血行再建は、血管内外科治療では対応できず、今後、頭蓋外-頭蓋内血行再建はさらに確固たる位置を占めると予想される。従って、バイパス手術は、脳卒中の外科治療の必須技術になると思われる。本稿では、バイパス手術の現状と将来を展望し、特に、最近当施設で実施しているICGを使用した術中血管撮影について述べる。(著者抄録)
  • 【脳卒中急性期管理Q&A チーム医療実践のために】脳卒中の治療戦略 脳出血の治療 脳内出血の外科的治療
    宮田 圭, 宝金 清博 救急・集中治療 20 (7-8) 999 -1003 2008年08月 [査読無し][通常論文]
  • 宝金 清博, 野中 雅, 飯星 智史 脳神経外科ジャーナル 17 (8) 572 -578 2008年08月 [査読無し][通常論文]
     
    血管内外科治療技術の進歩は,脳卒中の外科治療に大きな変化をもたらした.脳血行再建術においても,頭蓋外の動脈狭窄病変に対する治療では,その多くが血管内外科治療に置き換わりつつある.また,脳動脈瘤に対する血管内外科治療の適応は急速に広がりつつある.この結果,外科治療の対象となる動脈瘤は,単純な治療法で治療可能な動脈瘤ではなく,バイパスも含めた複雑な治療を必要とするものが相対的に増えている.また,慢性脳虚血に対するバイパスのエビデンスが確立しつつあり,頭蓋外-頭蓋内血行再建は血管内外科治療では対応できないものであり,今後,頭蓋外-頭蓋内血行再建はさらに確固たる位置を占めると予想される.したがってバイパス手術は,脳卒中の外科治療の必須要件になると思われる.本稿では,バイパス手術の現状と将来を展望したい.特に,術中血管撮影の登場,定量的血流測定の進歩,そして過灌流の画像診断などについて述べる.
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (7) 828 -833 2008年07月 [査読無し][通常論文]
  • 本望 修, 宝金 清博 脳神経外科ジャーナル 17 (7) 527 -530 2008年07月 [査読無し][通常論文]
     
    近年,神経再生に有用と思われる幹細胞が,神経系のみならず,その他の組織からも調整が可能となってきた.これらの幹細胞は,増殖や分化する能力が高いばかりでなく,移植後はホスト脳神経組織への親和性が高く,損傷部位へ遊走能し,状況に応じて分化するので,細胞治療の有力な候補となっている.自己の骨髄細胞を用いた脳梗塞治療などの場合,利点としては,発症よりある程度時間が経過してからでも治療の可能性があるということである.従来の治療のゴールデンタイムがきわめて短いことと比べると,注目すべきと思われる.現在の研究がさらに発展し,新治療方として成熟することが望まれる.
  • 患者自身の骨髄幹細胞移植による脳梗塞治療 国内初の臨床試験を実施して、大きな成果
    宝金 清博 臨床のあゆみ (76) 28 -29 2008年06月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (6) 690 -694 2008年06月 [査読無し][通常論文]
     
    第18回はtransjugular approachについて解説します。U.Fischの提唱するinfratemporal fossa approach,type Aに相当するものです。S状静脈洞、頸静脈球を除去する際、舌咽、迷走、副神経を保護するように内側壁を残し、外側壁のみを除去するのがポイントです。(著者抄録)
  • 石合 純夫, 本望 修, 寶金 清博, 森泉 茂宏, 土岐 めぐみ, 村上 孝徳, 本間 真理 リハビリテーション医学 : 日本リハビリテーション医学会誌 45 (0) S171 2008年05月18日 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (5) 560 -564 2008年05月 [査読無し][通常論文]
  • 当科における髄膜腫の傾向と病理
    秋山 幸功, 南田 善弘, 宝金 清博 Brain Tumor Pathology 25 (Suppl.) 138 -138 2008年05月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (4) 432 -439 2008年04月 [査読無し][通常論文]
  • 小柳 泉, 吉藤 和久, 村上 友宏, 大蔵 篤彦, 越智 さと子, 宝金 清博, 吉野 雅美, 飛騨 一利, 岩崎 喜信, 井須 豊彦 小児の脳神経 33 (2) 205 -205 2008年04月 [査読無し][通常論文]
  • 馬場 雄大, 黒田 敏, 宝金 清博 小児の脳神経 33 (2) 270 -270 2008年04月 [査読無し][通常論文]
  • 外傷性頭蓋内血腫に対する緊急的穿頭血腫洗浄術
    宮田 圭, 木下 園子, 浅井 康文, 飯星 智史, 秋山 幸功, 野中 雅, 小柳 泉, 宝金 清博 日本神経外傷学会プログラム・抄録集 31回 88 -88 2008年04月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (3) 298 -302 2008年03月 [査読無し][通常論文]
  • 宮田 圭, 飯星 智史, 秋山 幸功, 野中 雅, 小柳 泉, 宝金 清博, 浅井 康文 脳卒中 30 (2) 259 -259 2008年03月 [査読無し][通常論文]
  • 川村 麻衣子, 石黒 雅敬, 宝金 清博 脳卒中 30 (2) 312 -312 2008年03月 [査読無し][通常論文]
  • 野中 雅, 飯星 智史, 秋山 幸功, 原口 浩一, 宝金 清博 脳卒中の外科 36 (2) 88 -94 2008年03月 [査読無し][通常論文]
     
    頸部頸動脈狭窄症に対し、頸部頸動脈ステント留置術(CAS)治療を行った75歳以上の高齢者55症例60病変(高齢者群)の治療成績を75歳未満の120症例131病変(若年者群)と比較した。高齢者群は、全例、病変部にステントを留置でき、狭窄率が術前の平均81.9%から平均11.6%に改善した。mortalityは高齢者群:1.7%(1例)、若年者群:0%であった。morbidity rateは高齢者群:6.7%(4例)、若年者群:2.3%(3例)であった。高齢者群では内科的合併症を伴う率が高い傾向にあったが、若年者群との間に大きな差はみられなかった。しかし、無症候性の虚血性病変の発生など、潜在的な危険性は高いものと推測され、高齢者に対するCASの適応拡大においては新たな内科的治療群を含めた大規模臨床試験が必要であると思われた。
  • CEAのコツとpitfall
    宝金 清博 Medical Science Digest 34 (2) 46 -47 2008年02月 [査読無し][通常論文]
     
    内頸動脈血栓内膜剥離術は、大規模なRCTに基づき、その有用性、安全性が証明されている数少ない外科的手術手技である。その合併症率は5%以下であり、周術期の死亡率はほぼ0%とされている。しかし、いくつかのpitfallがあり、この手術は安易に行われてはいけない。特に、技術的には、脳虚血を避けるための内シャント挿入の基本的な技術が大前提である。また、日本人では、頸動脈病変が高位にあることも手術の難易度を高くしており、欧米の成績を単純に比較することも危険である。また、心血管系の合併症に十分に注意する必要がある。さらに、脳血流の低下した例では、術後の過灌流(hyperperfusion)に十分に注意する必要がある。最近では、心臓と同様にステントの有用性も明らかにされており、今後は、より高い安全性と良好な治療成績が要求されることは間違いない。(著者抄録)
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (2) 181 -189 2008年02月 [査読無し][通常論文]
  • Osamu Honmou, Kiyohiro Houkin, Takuya Matsunaga, Yoshiro Niitsu, Sumio Ishiai, Stephen G. Waxman, Jeffery D. Kocsis STROKE 39 (2) 543 -543 2008年02月 [査読無し][通常論文]
  • 三上 毅, 宝金 清博 Brain Nursing 24 (2) 120 -122 2008年02月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 18 (1) 42 -46 2008年01月 [査読無し][通常論文]
  • 脳梗塞に対する骨髄幹細胞治療
    本望 修, 宝金 清博 日本医事新報 (4367) 140 -141 2008年01月 [査読無し][通常論文]
  • 自己心拍再開した心肺停止くも膜下出血の臨床像
    宮田 圭, 加藤 航平, 浅井 康文, 飯星 智史, 野中 雅, 宝金 清博 日本脳神経外科救急学会プログラム・抄録集 13回 64 -64 2008年01月 [査読無し][通常論文]
  • Kiyohiro Houkin, Michael T. Lawton NEUROSURGICAL REVIEW 31 (1) 81 -81 2008年01月 [査読無し][通常論文]
  • 【脳梗塞 病期と病態に基づいた治療戦略】脳梗塞の最前線 骨髄幹細胞を用いた再生医療
    本望 修, 宝金 清博 カレントテラピー 25 (12) 1023 -1025 2007年12月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (12) 1406 -1412 2007年12月 [査読無し][通常論文]
     
    側頭下窩とは解剖学的な名称であり、実際の構造においては前方を下眼窩裂と上顎隆起、後方を側頭骨の乳突部、上方を蝶形骨大翼、下方を内側翼突筋、内側を蝶形骨の翼突板と斜台、外側を頬骨弓と下顎骨の下顎枝により囲まれています。しかし、外科的にinfratemporal fossa approach(ITFA)と言う際にはもっと広く、翼口蓋窩、側頭下窩、頸静脈孔など、中頭蓋底よりも尾側の病変に対するアプローチを指し、前方からのtransmaxillary approach、側方からのpreauricular ITFA、後方からのretroauricular ITFAの3つに大別されます。脳外科的には側方からアプローチすることが多いため、T.Fukushimaは新たなITFAの概念としてanterior,middle,posteriorの3つに分けて考えています。anterior ITFAは前側方から傍咽頭腔や斜台へアプローチするもので、翼口蓋窩から卵円孔まで、いわゆる解剖学的な側頭下窩の露出が可能です。middle ITFAは顎関節を除去し、真横から斜台へ到達するもので、卵円孔から茎状突起までの範囲を露出できます。posterior ITFAはtransjugular approachと同様で、茎状突起よりも後方で頸静脈孔周囲の病変へ到達可能です。第12回は前側方からのアプローチであるanterior ITFAについて解説します。これは前頭側頭開頭の延長として施行可能であり、傍咽頭腔や斜台方向へのlook downの視野、および脳底動脈先端部などのlook upの視野を得られるのが特徴です。(著者抄録)
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (11) 1282 -1286 2007年11月 [査読無し][通常論文]
  • 飯星 智史, 宮田 圭, 野中 雅, 宝金 清博 JNET: Journal of Neuroendovascular Therapy 1 (2) 210 -210 2007年11月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (10) 1158 -1165 2007年10月 [査読無し][通常論文]
  • 頸部内頸動脈高度(95%)狭窄症に対するステント留置術の治療成績
    飯星 智史, 原口 浩一, 野中 雅, 小柳 泉, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 66回 1G -4 2007年10月 [査読無し][通常論文]
  • 頸部脊椎症に伴う髄内浮腫性変化
    吉藤 和久, 小柳 泉, 馬場 雄大, 秋山 幸功, 飯星 智史, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 66回 2I -2 2007年10月 [査読無し][通常論文]
  • 頸椎変性疾患の手術治療における術後上肢症状発現の機序
    小柳 泉, 吉藤 和久, 村上 友宏, 馬場 雄大, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 66回 2I -6 2007年10月 [査読無し][通常論文]
  • 脳梗塞に対するAng-1-VEGF産生ヒト骨髄幹細胞移植による血管新生の検討
    外山 賢太郎, 本望 修, 鈴木 淳平, 宝金 清博, 濱田 洋文 日本脳神経外科学会総会CD-ROM抄録集 66回 2K -2 2007年10月 [査読無し][通常論文]
  • 脳梗塞に対するヒト骨髄幹細胞の多数回移植による治療効果の検討
    大森 義範, 本望 修, 鈴木 淳平, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 66回 2K -7 2007年10月 [査読無し][通常論文]
  • 心肺停止後全脳虚血に対する骨髄幹細胞移植の検討
    鄭 偉, 本望 修, 劉 赫, 原田 邦明, 鈴木 淳平, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 66回 2K -11 2007年10月 [査読無し][通常論文]
  • PFFEの複合めっき技術を用いた新しいBipolar forcepsの開発
    三上 毅, 柏原 茂樹, 南田 善弘, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 66回 2K -4 2007年10月 [査読無し][通常論文]
  • 脳神経外科領域における医療事故・合併症の定量的解析
    宝金 清博, 馬場 雄大, 小柳 泉, 野中 雅, 南田 善弘 日本脳神経外科学会総会CD-ROM抄録集 66回 3D -3 2007年10月 [査読無し][通常論文]
  • 脳梗塞に対する骨髄幹細胞治療
    本望 修, 宝金 清博 日本脳神経外科学会総会CD-ROM抄録集 66回 3I -7 2007年10月 [査読無し][通常論文]
  • 脳梗塞に対する自己骨髄幹細胞治療へ向けて
    本望 修, 宝金 清博, 松永 卓也, 新津 洋司郎, 山村 明範 脳循環代謝 19 (2) 113 -113 2007年10月 [査読無し][通常論文]
  • Kiyohiro Houkin NEUROSURGICAL REVIEW 30 (4) 305 -305 2007年10月 [査読無し][通常論文]
  • 黒田 敏, 中山 若樹, 岩崎 喜信, 石川 達哉, 宝金 清博, 上山 博康 日本脳神経外科学会総会CD-ROM抄録集 66回 1F -1 2007年10月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (9) 1038 -1043 2007年09月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (8) 912 -916 2007年08月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (7) 815 -821 2007年07月 [査読無し][通常論文]
  • 小柳 泉, 吉藤 和久, 秋山 幸功, 宝金 清博, 今村 博幸, 藤本 真, 飛騨 一利, 岩崎 喜信 Neurosurgical Emergency 12 (1) 43 -48 2007年07月 [査読無し][通常論文]
     
    機械的損傷に引き続いて起こる二次損傷を最小限に抑えることは、急性脊髄損傷治療の重要な目的の一つである。近年、米国および日本から急性頸椎頸髄損傷の治療に関するガイドラインが出版された。これは、厳密な文献の検証によるものであるが、大部分は日本以外からの文献である。我が国では、骨折・脱臼を伴わない急性頸髄損傷が、頸髄損傷全体の約半数を占めており、特徴的な病態となっている。本論文では、我々の経験と米国および日本でのガイドラインの記載から、骨損傷のない頸髄損傷を中心として、病態および治療に関して検討した。いずれのガイドラインも多施設臨床研究の結果にもかかわらずステロイドの使用には、その合併症のため注意を促していた。現在行われている脊髄損傷治療の有効性を確認するためには、今後もエビデンスの積み重ねが必要と思われる。(著者抄録)
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (6) 694 -699 2007年06月 [査読無し][通常論文]
  • 【虚血性脳卒中診療の最前線】脳卒中診療体制 脳卒中リスクマネジメントの意義
    宝金 清博 Clinical Neuroscience 25 (6) 708 -709 2007年06月 [査読無し][通常論文]
  • 馬場 雄大, 原口 浩一, 野中 雅, 宝金 清博 CI研究 29 (1) 27 -32 2007年06月 [査読無し][通常論文]
     
    海綿静脈洞部硬膜動静脈瘻(dCCF)3例に対し、頸動脈の回転digital subtraction angiography(DSA)を用いてshunt pointの同定を試みた。観察対象はshunt pointであったため、撮影は基本的に動脈相とし、多くの流出路の存在による画像の複雑化を回避した。3D再構成画像では、全例にparasinus様の構造を認め、中頭蓋窩、及び後頭蓋窩硬膜からの流入動脈が海綿静脈洞後内側部、もしくはposterior intercavernous sinusに集簇する構造を確認した。DSAはシャント部の同定には有用だと思われたが、治療において、1例は比較的コンパクトな塞栓で治癒を得たものの、2例は流出経路の変化のため両側海綿静脈洞を閉塞せざるを得なかった。
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (5) 572 -575 2007年05月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (4) 428 -433 2007年04月 [査読無し][通常論文]
  • 中川原 譲二, 宝金 清博 脳神経外科速報 17 (4) 398 -408 2007年04月 [査読無し][通常論文]
  • Oligodendrogliomaにおける遺伝子マーカー1p,19qの検討
    秋山 幸功, 南田 善弘, 佐々木 文, 宝金 清博 Brain Tumor Pathology 24 (Suppl.) 82 -82 2007年04月 [査読無し][通常論文]
  • 延髄腹側に発生したEndodermal cystの一例
    今井 浩平, 秋山 幸功, 南田 善之, 宝金 清博 Brain Tumor Pathology 24 (Suppl.) 116 -116 2007年04月 [査読無し][通常論文]
  • 小柳 泉, 村上 友宏, 吉藤 和久, 宝金 清博, 飛騨 一利, 岩崎 喜信, 井須 豊彦 小児の脳神経 32 (2) 137 -137 2007年04月 [査読無し][通常論文]
  • 越智 さと子, 村上 友宏, 小柳 泉, 宝金 清博 小児の脳神経 32 (2) 139 -139 2007年04月 [査読無し][通常論文]
  • 村上 友宏, 小柳 泉, 宝金 清博 小児の脳神経 32 (2) 142 -142 2007年04月 [査読無し][通常論文]
  • 今井 浩平, 村上 友宏, 小柳 泉, 宝金 清博 小児の脳神経 32 (2) 201 -201 2007年04月 [査読無し][通常論文]
  • 大石 英則, 木内 博之, 塩川 芳昭, 宝金 清博, 宮地 茂 Medical Torch 3 (2) 7 -19 2007年04月 [査読無し][通常論文]
  • 【高難度脳動脈瘤治療の現状と将来】脳動脈瘤治療 分岐点か通過点か
    宝金 清博 Medical Torch 3 (2) 20 -21 2007年04月 [査読無し][通常論文]
  • Retroodontoid massを伴ったC1脊柱管狭窄を呈した高齢者3例の治療経験
    村上 友宏, 小柳 泉, 吉藤 和久, 宝金 清博 日本脊髄障害医学会雑誌 20 (1) 122 -123 2007年04月 [査読無し][通常論文]
  • Kiyohiro Houkin, Yukinori Akiyama, Nobuo Hashimoto, Kazuhiro Nozaki JOURNAL OF NEUROSURGERY 106 (4) A753 -A753 2007年04月 [査読無し][通常論文]
  • 原口 浩一, 馬場 雄大, 野中 雅, 宝金 清博 新潟医学会雑誌 121 (4) 226 -226 2007年04月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 16 (4) 321 -321 2007年04月 [査読無し][通常論文]
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (3) 310 -313 2007年03月 [査読無し][通常論文]
  • 外山 賢太郎, 今泉 俊雄, 高橋 明, 大山 浩史, 大坊 雅彦, 原口 浩一, 宝金 清博 脳卒中 29 (2) 280 -280 2007年03月 [査読無し][通常論文]
  • 宮田 圭, 野中 雅, 斉藤 修, 村上 友宏, 秋山 幸功, 原口 浩一, 馬場 雄大, 南田 善弘, 小柳 泉, 宝金 清博 脳卒中 29 (2) 335 -335 2007年03月 [査読無し][通常論文]
  • 恩田 敏之, 本望 修, 原田 邦明, 宝金 清博, 濱田 洋文 脳卒中 29 (2) 429 -429 2007年03月 [査読無し][通常論文]
  • 宝金 清博 日本内分泌学会雑誌 82 (4) 844 -845 2007年03月 [査読無し][通常論文]
  • 骨折・脱臼のない急性頸髄損傷における傍脊柱軟部組織損傷の臨床的意義
    小柳 泉, 村上 友宏, 吉藤 和久, 宝金 清博, 藤本 真, 今村 博幸, 飛騨 一利, 岩崎 喜信 日本神経外傷学会プログラム・抄録集 30回 76 -76 2007年03月 [査読無し][通常論文]
  • 頭部外傷における外傷性微小点状出血
    宮田 圭, 小柳 泉, 宝金 清博 日本神経外傷学会プログラム・抄録集 30回 86 -86 2007年03月 [査読無し][通常論文]
  • 外傷後の頸部痛で発症した後頭骨腫瘍の1小児例
    村上 友宏, 小柳 泉, 宮田 圭, 宝金 清博, 鈴木 信寛 日本神経外傷学会プログラム・抄録集 30回 119 -119 2007年03月 [査読無し][通常論文]
  • 急性頸髄損傷をきたした骨形成不全症の一例
    今井 浩平, 村上 友宏, 小柳 泉, 宝金 清博, 岡本 博之, 浅井 康文 日本神経外傷学会プログラム・抄録集 30回 121 -121 2007年03月 [査読無し][通常論文]
  • 鐙谷武雄, 寶金清博, 福原俊, 森田智視 脳卒中 29 (2) 284 2007年03月01日 [査読無し][通常論文]
  • 南田 善弘, 寶金 清博 日本臨床 65 (0) 573 -578 2007年03月 [査読無し][通常論文]
  • 野中 雅, 岡 真一, 原口 浩一, 馬場 雄大, 宝金 清博 脳卒中の外科 35 (2) 95 -100 2007年03月 [査読無し][通常論文]
     
    頸部頸動脈ステント留置術(CAS)を行った77症例88病変について、遷延性低血圧(収縮期血圧90mmHg以下が3時間以上持続)発生の予測因子の解析を行った。CAS後の低血圧が44病変で起こり、21病変でカテコールアミン投与を行った。虚血性合併症は一過性の意識障害、構音障害が5病変に出現したが、永久的な精神障害は認められなかった。頸動脈分岐部の石灰化のみが独立した有意な遷延性低血圧の予測因子となり、年齢、性別、病変側(右左)、狭窄改善度と遷延性低血圧との間に相関性は認められなかった。一方、使用した4種のステントのうちでSART(eR)とProtege使用例で遷延性低血圧の発生頻度が高かった。頸動脈分岐部の石灰化を伴う病変では術後低血圧が遷延する危険性が高いので、血圧管理に留意する必要がある。
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (2) 174 -178 2007年02月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科速報 17 (2) 179 -183 2007年02月 [査読無し][通常論文]
  • Akira Tamura, Takahiro Amano, David G. Sherman, Kiyohiro Houkin, Takao Asano, Yasuo Fukuuchi, Takenori Yamaguchi, Nobuo Hashimoto, Syotai Kobayashi, Setsuro Ibayashi, Dai Kitao, Hitoshi Kajitani STROKE 38 (2) 511 -512 2007年02月 [査読無し][通常論文]
  • 馬場 雄大, 原口 浩一, 野中 雅, 小柳 泉, 宝金 清博, 山口 岳彦 小児の脳神経 32 (1) 20 -23 2007年02月 [査読無し][通常論文]
     
    症例は9歳女児。軽微な頭部外傷の検査目的で施行したCTにて異常を指摘され、精査の結果脳動脈瘤の診断を得た。7mm大の大きさの脳動脈瘤ではあるが強い石灰化を伴っており手術にて脳動脈瘤を摘出した。病理では血栓化巨大脳動脈瘤の増大機序に類似した変化を認めた。小児の脳動脈瘤は成人の脳動脈瘤と比べ稀な疾患であり、部位、大きさ、形態などにおいて異なる特徴を有するが、原因や自然歴については不明な点が多い。小児の無症候性未破裂動脈瘤においても治療法、治療時期の選択に関して検討を必要とすることが示唆された。(著者抄録)
  • 鰐渕 昌彦, 福島 孝徳, Friedman Allan H., 宝金 清博 脳神経外科速報 17 (1) 41 -45 2007年01月 [査読無し][通常論文]
  • 当施設における航空機による神経外傷救急搬送
    宮田 圭, 奈良 理, 岡本 博之, 井上 弘行, 浅井 康文, 宝金 清博 日本脳神経外科救急学会プログラム・抄録集 12回 121 -121 2007年01月 [査読無し][通常論文]
  • 原口 浩一, 宝金 清博 分子脳血管病 6 (1) 104 -107 2007年01月 [査読無し][通常論文]
     
    ISATによるとくも膜下出血後1年における転帰は、コイル塞栓術がクリッピングにくらべ7.4%良好とされたが、その後の再破裂率はコイル塞栓術群で多かった。本研究では中長期的にみてその後の再破裂率がコイル塞栓術群でやや高いものの有意差はなく、最初に得られた良好な転帰が高い再破裂率により覆るものではないことが示された。(著者抄録)
  • 小柳 泉, 村上 友宏, 宝金 清博 脳神経外科ジャーナル 16 (1) 18 -25 2007年01月 [査読無し][通常論文]
     
    急性頸椎頸髄損傷の臨床ガイドラインが,米国および日本でも出版された.しかし,脊椎脊髄損傷の治療に関してエビデンスレベルの高い臨床研究はきわめて少なく,明確な指標を示すというよりは,治療optionの提示となっている.本論文では,わが国における頸椎頸髄損傷の実際と治療の問題点に関して,ガイドラインの記載を基にエビデンスのレビューを行った.日本では,高齢者での骨損傷のない頸髄損傷は,北米に比べて高い頻度でみられるが,この病態に対する治療方針は,いまだ標準化されていない.また,ガイドラインは,メチルプレドニゾロンの大量療法に関しては,注意深い使用を促している.これは,根拠となったランダム化臨床試験での分析方法に問題があることや,ステロイドによる合併症のためである.急性脊椎脊髄損傷に対して現在行われている治療手段の有効性を判断し,さらに明確なガイドラインを作成していくためには,今後もエビデンスの積み重ねが必要と考える.
  • 本望 修, 宝金 清博 糖尿病 = Journal of the Japan Diabetes Society 49 (12) 911 -913 2006年12月30日 [査読無し][通常論文]
  • 堀田 祥史, 本望 修, 原田 邦明, 寶金 清博, 濱田 洋文 脳卒中 28 (4) 612 -612 2006年12月25日 [査読無し][通常論文]
  • 前原 忠行, 寶金 清博 脳卒中 28 (4) 678 -678 2006年12月25日 [査読無し][通常論文]
  • Neurogenesisと神経再生医療 脳梗塞に対する骨髄幹細胞移植による脳循環の改善
    本望 修, 鵜飼 亮, 原田 邦明, 宝金 清博 脳循環代謝 18 (4) 189 -191 2006年12月 [査読無し][通常論文]
     
    脳梗塞モデルラットに対し、骨髄間葉系幹細胞(MSC)を経静脈的に投与すると、梗塞巣の縮小化や神経機能の改善が認められることが知られているが、脳血流に関する詳細な検討は報告されていない。今回、我々は、脳梗塞後1週間にわたり、脳梗塞周囲の組織の血流を解析し、治療機序の解明を試みた。ラット脳梗塞モデルは中大脳動脈永久閉塞モデルを用いた。経時的に高磁場(7T)MRIを用いて、DWI、T2WI、PWI(rCBV)を撮影し、脳血流と組織ダメージとの関連性を経時的に解析した。また、MSC移植群との比較解析を行い、治療効果を検討した。結果は、ischemic coreの脳血流は、極期においてもゼロにはならず、早期より改善傾向を呈したことより、細胞治療時の細胞デリバリールートとして体循環を利用する戦略が期待された。また、MSC移植治療により脳血流が著明に改善したことより、作用機序の一つに血管新生が関係していると思われた。(著者抄録)
  • 本望 修, 宝金 清博 糖尿病 49 (12) 911 -913 2006年12月 [査読無し][通常論文]
  • 原口 浩一, 寶金 清博 日本臨床 64 (0) 361 -363 2006年11月 [査読無し][通常論文]
  • Big Debate 巨大paraclinoid meningioma
    南田 善弘, 宝金 清博 脳神経外科速報 16 (10) 883 -883 2006年10月 [査読無し][通常論文]
  • 小柳 泉, 村上 友宏, 馬場 雄大, 宝金 清博, 小野 誠司 臨床神経生理学 34 (5) 465 -465 2006年10月 [査読無し][通常論文]
  • 経上腕動脈経由による頸動脈ステント留置術
    原口 浩一, 野中 雅, 宝金 清博 日本血管外科学会雑誌 15 (6) 583 -583 2006年10月 [査読無し][通常論文]
  • Izumi Koyanagi, Kiyohiro Houkin NEUROSURGICAL REVIEW 29 (4) 264 -264 2006年10月 [査読無し][通常論文]
  • 三上 毅, 南田 善弘, 氷見 徹夫, 宝金 清博 Neurological Surgery 34 (9) 933 -937 2006年09月 [査読無し][通常論文]
     
    66歳女。生下時より左内耳奇形と鼓膜欠損を指摘されており、26歳時に左中耳炎根治術を受けた既往があり、発熱と頭痛を契機に近医にて髄膜炎として加療を受けた。その半年後に発熱、頭痛に髄液耳漏が生じ、近医にて入院加療を受け、4日後に髄液漏が止まり、紹介となった。神経学的異常は生下時からなく、CT及びMRIでは外側半規管と後半規管は殆ど形成されていなかった。内耳奇形を伴う耳性髄液漏との診断にて経迷路的に髄液漏根治術を行った。アブミ骨を摘出したところ、前庭窓からの髄液の噴出が確認できた。前庭を開放した後で側頭筋膜を充填し、フィブリングルーで固定した。その後、約1年間は髄液漏の再発はなく経過観察中である。
  • Kiyohiro Houkin NEUROLOGIA MEDICO-CHIRURGICA 46 (9) 428 -428 2006年09月 [査読無し][通常論文]
  • Kiyohiro Houkin NEUROLOGIA MEDICO-CHIRURGICA 46 (9) 468 -468 2006年09月 [査読無し][通常論文]
  • 秋山 幸功, 南田 義弘, 三上 毅, 宝金 清博 Brain Tumor Pathology 23 (Suppl.) 97 -97 2006年06月 [査読無し][通常論文]
  • Lymphomatosis cerebriを呈したanaplastic large cell lymphomaの一例
    杉野 寿哉, 南田 善弘, 三上 毅, 宝金 清博 Brain Tumor Pathology 23 (Suppl.) 151 -151 2006年06月 [査読無し][通常論文]
  • 宝金 清博, 野中 雅, 原口 浩一 血管医学 7 (2) 169 -175 2006年05月 [査読無し][通常論文]
     
    無症候の脳動脈狭窄・閉塞に対する血行再建に関するレビューを行った.頸部頸動脈の狭窄に関しては,60%以上の狭窄がある場合,外科的な血栓内膜剥離術は脳卒中予防に有効である.ただし,重症脳卒中発症や死亡率を改善することはなかった.狭窄度と脳卒中発症率の間に,明確な関連は証明されなかった.血栓内膜剥離術がよいか,ステント治療がよいかは,不明である.狭窄率以外のパラメータ,すなわちプラークの性状を見る必要があり,MR-Black blood法が有用である.頭蓋内動脈の狭窄に関しては,無症候の場合には,治療を薦めるエビデンスはない.ただ,閉塞があり,脳循環検査でmisery perfusion(貧困灌流)が存在する場合には,血行再建(STA-MCA)が考慮されても構わない(著者抄録)
  • 小柳 泉, 吉藤 和久, 馬場 雄大, 南田 善弘, 宝金 清博, 岩崎 喜信, 飛騨 一利, 矢野 俊介, 井須 豊彦 小児の脳神経 31 (2) 119 -119 2006年04月 [査読無し][通常論文]
  • 吉藤 和久, 小柳 泉, 馬場 雄大, 原口 浩一, 秋山 幸功, 山下 太郎, 小松 克也, 杉野 寿哉, 池田 英之, 宝金 清博 小児の脳神経 31 (2) 150 -150 2006年04月 [査読無し][通常論文]
  • Koyanagi, I, K Yoshifuji, T Baba, Y Akiyama, K Houkin, K Hida, Y Iwasaki JOURNAL OF NEUROSURGERY 104 (4) A653 -A653 2006年04月 [査読無し][通常論文]
  • K Houkin, TK Baba, T Nonaka JOURNAL OF NEUROSURGERY 104 (4) A645 -A645 2006年04月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 15 (4) 325 -325 2006年04月 [査読無し][通常論文]
  • 秋山 幸功, 宝金 清博, 野中 雅, 馬場 雄大 脳卒中 28 (1) 133 -133 2006年03月 [査読無し][通常論文]
  • 馬場 雄大, 宝金 清博, 野中 雅 脳卒中 28 (1) 139 -139 2006年03月 [査読無し][通常論文]
  • 山村 明範, 寺川 雄三, 布村 克幸, 村山 直昭, 藤重 正人, 中川 俊男, 端 和夫, 宝金 清博 脳卒中 28 (1) 147 -147 2006年03月 [査読無し][通常論文]
  • 金 相年, 本望 修, 宝金 清博 脳卒中 28 (1) 199 -199 2006年03月 [査読無し][通常論文]
  • 秋山 幸功, 山村 明範, 中川 俊男, 宝金 清博 脳卒中 28 (1) 220 -220 2006年03月 [査読無し][通常論文]
  • 片岡 大治, 野崎 和彦, 宝金 清博 脳神経外科速報 16 (2) 175 -179 2006年02月 [査読無し][通常論文]
  • 宝金 清博, 馬場 雄大, 小柳 泉 脳神経外科ジャーナル 15 (2) 89 -96 2006年02月 [査読無し][通常論文]
     
    脳神経外科に限らず, リスクマネジメントは, 臨床における最も急務の課題である.ただ, 一般的な分析やリスク解析が非常に進んでいる一方で, 個別の具体的な問題の解析が非常に遅れていることも事実である.札幌医科大学脳神経外科におけるインシデンスの解析を行った.インシデンスの90%以上が病棟業務に関連しており, 中でも, 中心静脈, 脳脊髄液ドレナージなどに関するインシデンスが主要なものであった.手術では, 一般的な手術リスクに加えて異物残存, 付帯的なインシデンスがみられた.検査では, 脳血管撮影に伴うリスクが重要である.外来では, 診断の遅れがリスクとなる.今後, 脳神経外科が, 社会の中でより信頼される診療科となるためには, こうしたインシデンスの科学的な解析が必須であり, そのためには, データの集積が不可欠である.
  • 大坂 美鈴, 野中 雅, 岡 真一, 南田 善弘, 三上 毅, 宝金 清博 脳と神経 58 (1) 57 -61 2006年01月 [査読無し][通常論文]
     
    33歳女.患者は頭痛と抑うつ状態を主訴とした.はじめ全身性エリテマトーデスの診断でプレドニゾロンの内服治療が開始されていた.抗核抗体および抗DNA抗体RIA,抗Ds-DNA抗体は陽性,眼底検査ではうっ血乳頭はなく,慢性頭蓋内圧亢進所見も認められなかった.しかし,頭部造影CTでは硬膜肥厚および上矢状洞内視鏡にempty delta signを認め,上矢状洞閉塞に伴う頭蓋内圧亢進症状がないため,保存的に治療を行った.入院後の髄液検査ではIL-6の上昇,抗神経細胞抗体陽性,IgG Indexは高値を示した.中枢神経ループスと診断され,その後,頭痛の改善を認め独歩退院となった
  • 脳神経外科における安全管理
    宝金 清博 日本脳神経外科救急学会プログラム・抄録集 11回 53 -53 2006年01月 [査読無し][通常論文]
  • 急性期脊髄損傷に対する治療 急性期頸椎頸髄損傷に対する治療 ガイドラインから
    小柳 泉, 吉藤 和久, 秋山 幸功, 宝金 清博, 今村 博幸, 飛騨 一利, 岩崎 喜信 日本脳神経外科救急学会プログラム・抄録集 11回 69 -69 2006年01月 [査読無し][通常論文]
  • 病院到着前心停止患者に対する早期除細動および循環補助療法の神経機能予後について
    秋山 幸功, 長谷 守, 浅井 康文, 宝金 清博 日本脳神経外科救急学会プログラム・抄録集 11回 89 -89 2006年01月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 15 (9) 646 -646 2006年 [査読無し][通常論文]
  • 小柳 泉, 宝金 清博, 馬場 雄大, 吉藤 和久, 今村 博幸, 藤本 真, 飛騨 一利, 岩崎 喜信 神経外傷 28 (1) 22 -26 2005年12月27日 [査読無し][通常論文]
  • 原口 浩一, 野中 雅, 宝金 清博 Progress in Medicine 25 (12) 3151 -3154 2005年12月 [査読無し][通常論文]
     
    DPC(診断群分類別包括評価)と呼ばれる新制度が臨床現場にもたらす影響を,くも膜下出血を例に挙げ,コスト面の分析とともに考察した.DPCは,米国で既に実施されているDRG/PPSを基に開発された日本独自の制度で,米国と異なる点は,米国が入院1件当たりで算定するのに対し,日本では入院患者1日当たりの定額払いになる.効率的なコスト管理が求められ,特に使用薬剤は投与日数による一律使用ではなく,治療効率への配慮と,症状に応じたきめ細かい使い分けが必要になる.43歳女.右中大脳動脈瘤を認め,緊急開頭クリッピング術を施行した.脳血管攣縮予防のために塩酸ファスジルを術後14日間投与し,通常の術後管理を行った.経過は良好で,独歩退院した.312761点が包括診療での請求額,従来の出来高評価では306848点でそれほど違いはない.しかしながら,包括評価での診療報酬明細書は驚くほど簡潔で,医事業務の大幅な軽減になる
  • 馬場 雄大, 三上 毅, 南田 善弘, 宝金 清博 核医学 42 (4) 422 -422 2005年12月 [査読無し][通常論文]
  • K Kurozumi, K Nakamura, T Ichikawa, T Tamiya, Y Ito, O Honmou, K Houkin, H Hamada, Date, I JOURNAL OF GENE MEDICINE 7 (12) 1586 -1587 2005年12月 [査読無し][通常論文]
  • 宝金 清博 日本医事新報 0 (4260) 8 -14 2005年12月 [査読無し][通常論文]
  • 三上 毅, 南田 善弘, 八巻 稔明, 馬場 雄大, 宝金 清博 脳神経外科ジャーナル 14 (11) 683 -688 2005年11月 [査読無し][通常論文]
     
    2003〜2005年に神経血管減圧術20例を対象に,Fast imaging employing steady-state acquisition(FIESTA)とspoiled gradient-recalled acquisition(SPGR)にて術前後の圧迫血管を評価し,術中所見との相違性を検討した.疾患は三叉神経痛6例(平均58.5歳),顔面痙攣14例(平均53.4歳)であった.その結果,術前評価はいずれの疾患においても動脈による圧迫がFIESTAおよびSPGRで確認された.一方,三叉神経痛における静脈による圧迫2例のうち1例はFIESTAのみで確認された.術中所見は術前と同様であり,術後評価は挿入したprosthesisがFIESTAのみで確認された.FIESTAは術後のprosthesisと血管,root exit zoneの相互的位置関係の評価に有用であると考えられた
  • 馬場 雄大, 宝金 清博 脳神経外科速報 15 (10) 904 -904 2005年10月 [査読無し][通常論文]
  • 脳梗塞の治療戦略と今後の展望 脳梗塞急性期,画像診断のup date 脳梗塞急性期の画像診断 総括
    宝金 清博 Medical Postgraduates 43 (4) 410 -413 2005年10月 [査読無し][通常論文]
  • K Houkin NEUROSURGICAL REVIEW 28 (4) 248 -248 2005年10月 [査読無し][通常論文]
  • 南田 善弘, 三上 毅, 八巻 稔明, 端 和夫, 寶金 清博 日本内分泌学会雑誌 81 19 -21 2005年09月20日 [査読無し][通常論文]
  • 馬場 雄大, 宝金 清博 脳神経外科速報 15 (9) 874 -874 2005年09月 [査読無し][通常論文]
  • 八巻 稔明, 宝金 清博 診断と治療 93 (9) 1583 -1587 2005年09月 [査読無し][通常論文]
  • T Mikami, Y Minamida, K Ohtsuka, K Houkin ACTA NEUROCHIRURGICA 147 (9) 1005 -1006 2005年09月 [査読無し][通常論文]
     
    A 67-year-old man was suffering from a microrotary deviation of the left eye for more than 11 years. He had difficulty fixating from the onset. 8 years after onset he was diagnosed with SOM. He was also treated with carbamazepine, phenytoin and baclofen, but his symptoms were not resolved. On admission, he had continuous unilateral, tonic intorsional nystagmus and phasic oscillations of the left eye. The nystagmus was reinforced when he gazed down. Symptoms were exacerbated by stress and fatigue. With the patient prone, his head was rotated to the right and an occipital interhemispheric transtentorial approach was performed. After opening the quadrigeminal cistern, the origin of the trochlear nerve was revealed to be held suspended by the branch of the SCA (superior cerebellar artery) (diameter <1mm). The thinnest part of the nerve was located 1.2mm from the exit point and was twisted 90 degrees. Here, the REZ was compressed at the ventral side of the nerve (Fig. 1). In this case, the compressing small branch was from the caudal trunk of the SCA. By fixing the distortion of the caudal trunk at the proximal portion using Teflon felt, the position of the vessel was shifted distal from the REZ, and the suspended trochlear nerve was loosened (Fig. 1). In this case, it was only possible to shift the compressing vessel 1mm distally because the branch leading to the brainstem had become stretched and came close to being cut. Involuntary rotary eye movement disappeared completely just after the operation, and there was no postoperative neurological deficit. The patient has had no recurrence for 18 months.
  • 馬場 雄大, 寶金 清博 救急医学 29 (9) 1087 -1092 2005年09月 [査読無し][通常論文]
  • 原田 邦明, 本望 修, 宝金 清博 分子脳血管病 4 (3) 293 -297 2005年07月 [査読無し][通常論文]
     
    MRIは神経系再生医療において重要な役割を果たす.われわれは動物実験用MRI装置を用いて,とくに拡散強調画像,拡散テンソル画像について研究し,神経再生がもたらす梗塞巣の経時的変化の解析に応用している.また最近,骨髄細胞に金属粒子を標識しドナー細胞のホスト内での追跡画像について注目が高まっている(著者抄録)
  • 迷走神経刺激療法が著効したLennox-Gastaut症候群の1女児例
    若井 周治, 二階堂 弘輝, 堤 裕幸, 本望 修, 宝金 清博, 川本 芳誉 日本小児科学会雑誌 109 (7) 860 -860 2005年07月 [査読無し][通常論文]
  • 【脳血管障害のマネジメント プライマリケアにおける一次予防を中心に】トピックス 包括診療のなかの脳卒中診療
    野中 雅, 宝金 清博 クリニカルプラクティス 24 (5) 547 -550 2005年05月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 14 (4) 248 -248 2005年04月 [査読無し][通常論文]
  • Ossified craniopharyngiomaの3例
    三上 毅, 南田 善弘, 八巻 稔明, 池田 健, 宝金 清博 Brain Tumor Pathology 22 (Suppl.) 86 -86 2005年04月 [査読無し][通常論文]
  • 視床下部に発生したCapillary hemangiomaの1例
    宮田 至朗, 南田 善弘, 三上 毅, 野中 雅, 小柳 泉, 宝金 清博, 池田 健 Brain Tumor Pathology 22 (Suppl.) 88 -88 2005年04月 [査読無し][通常論文]
  • 宝金 清博, 野中 雅, 馬場 雄大, 黒田 敏 小児の脳神経 30 (2) 122 -122 2005年04月 [査読無し][通常論文]
  • 小柳 泉, 宝金 清博, 南田 善弘, 岩崎 喜信, 飛騨 一利, 矢野 俊介, 井須 豊彦 小児の脳神経 30 (2) 180 -180 2005年04月 [査読無し][通常論文]
  • 吉藤 和久, 外山 賢太郎, 宮田 圭, 今泉 俊雄, 小柳 泉, 宝金 清博 小児の脳神経 30 (2) 192 -192 2005年04月 [査読無し][通常論文]
  • 馬場 雄大, 三上 毅, 南田 善弘, 岡 真一, 宮田 至朗, 宝金 清博 小児の脳神経 30 (2) 200 -200 2005年04月 [査読無し][通常論文]
  • 劉 赫, 本望 修, 原田 明邦, 宝金 清博, 濱田 洋文 脳卒中 27 (1) 168 -168 2005年04月 [査読無し][通常論文]
  • Koyanagi, I, Y Iwasaki, K Hida, K Houkin SURGICAL NEUROLOGY 63 (4) 350 -355 2005年04月 [査読無し][通常論文]
     
    Background: Syringomyelia is a common intramedullary lesion associated with spinal arachnoiditis and obstruction of the foramen magnum such as in Chiari's malformation. Disturbance of cerebrospinal fluid flow around the spinal cord has an important role in the development of syringomyelia due to spinal arachnoiditis; however, the exact mechanisms have not been clarified. The purpose of this retrospective study is to understand the clinical features and pathomechanisms of syringomyelia secondary to spinal arachnoiditis and to provide the current choice of surgical treatment in this difficult clinical entity. Methods: Clinical and radiological findings in 15 patients with syringomyelia associated with spinal arachnoiditis who underwent surgical treatment in our institutes between 1982 and 2000 were reviewed. All patients presented with paraparesis or tetraparesis on admission. Results: Magnetic resonance imaging (MRI) or computed tomography-myelography revealed that the syrinx predominantly existed at the thoracic levels. Five patients showed complete block of the thoracic subarachnoid space by conventional myelography. T2-weighted MRI showed diffuse intramedullary hyperintensity at the level of arachnoiditis. As the first surgical treatment, 10 patients underwent syringo-peritoneal shunt placement. Three patients were treated with a syringo-subarachnoid shunt, and 2 patients were treated with a ventriculoperitoneal shunt. Eight patients required further shunting operations for syringomyelia 2 months to 12 years after the first surgery. Neurologic improvement was obtained in 9 patients (60%) with decreased size of the syrinx. One patient remained stable; 5 patients showed gradual deterioration. Conclusions: The syrinx originated from the thoracic levels where severe adhesion of the subarachnoid space was present. The mechanisms of syrinx formation may be based on the increased interstitial fluid in the spinal cord. Shunting procedures were effective in some population of the patients. Decompression procedures of the spinal subarachnoid space may be an alternative primary surgical treatment except for patients with longitudinally extensive arachnoiditis. (c) 2005 Elsevier Inc. All rights reserved.
  • 南田 善弘, 野中 雅, 寶金 清博 日本薬剤師会雑誌 57 (4) 473 -477 2005年04月 [査読無し][通常論文]
  • 馬場 雄大, 宝金 清博 脳神経外科速報 15 (3) 221 -226 2005年03月 [査読無し][通常論文]
  • 脊髄損傷の病態と治療 頸椎後縦靱帯骨化症における脊椎管サイズと脊髄損傷の関連
    小柳 泉, 宝金 清博, 馬場 雄大, 今村 博幸, 藤本 真, 飛騨 一利, 岩崎 喜信 日本神経外傷学会プログラム・抄録集 28回 99 -99 2005年03月 [査読無し][通常論文]
  • H Hamada, M Kobune, K Nakamura, Y Kawano, K Kato, O Honmou, K Houkin, T Matsunaga, YN Niitsu CANCER SCIENCE 96 (3) 149 -156 2005年03月 [査読無し][通常論文]
     
    We developed human mesenchymal stem cell (MSC) lines that could differentiate into various tissue cells including bone, neural cells, bone marrow (BM) stromal cells supporting the growth of hematopoietic stem cell (HSC), and so-called 'tumor stromal cells' mixing with tumor cells. We investigated the applicability of MSC as therapeutic cell transplanting reagents (cytoreagents). Telomerized human BM derived stromal cells exhibited a prolonged lifespan and supported the growth of hematopoietic clonogenic cells. The gene transfer of Indian hedgehog (Ihh) remarkably enhanced the HSC expansion supported by the human BM stromal cells. Gene-modified MSC are useful as therapeutic tools for brain tissue damage (e.g. brain infarction) and malignant brain neoplasms. MSC transplantation protected the brain tissue from acute ischemic damage in the midcerebral artery occlusion (MCAO) animal model. Brain-derived neurotrophic factor (BDNF)-gene transduction further enhanced the protective efficacy against the ischemic damage. MSC possessed excellent migratory ability and exerted inhibitory effects on the proliferation of glioma cells. Gene-modification of MSC with therapeutic cytokines clearly augmented the antitumor effect and prolonged the survival of tumor-bearing animals. Gene therapy employing MSC as a tissue-protecting and targeting cytoreagent would be a promising approach.
  • 七戸 秀夫, 黒田 敏, 浅野 剛, 牛越 聡, 石川 達哉, 宝金 清博, 村下 十志文, 岩崎 喜信 Neurological Surgery 33 (2) 149 -153 2005年02月 [査読無し][通常論文]
     
    症例1:49歳男.構音障害と右不全麻痺で発症し,両側内頸動脈に90%狭窄を認めると共に,慢性心不全,冠動脈三枝疾患の合併が判明した.冠動脈バイパス術(CABG)単独では両側大脳半球の灌流圧が著明に低下する危険が高いと考え,まず無症候性の右内頸動脈起始部の狭窄病変に対してstentを用いた血管形成術を施行した.翌日に左頸動脈内膜剥離術(CEA)および冠動脈バイパス術(CABG)を同時に行った.術後頸動脈の狭窄は改善し,両側性に脳血流量の増加を認め,5年経過し再発はない.症例2:62歳男.2年前,労作性狭心症でstentを併用した血管形成術を施行された.しかし,冠動脈狭窄が再発してCABGの適応となり,術前スクリーニングで両側の頸部内頸動脈に狭窄を指摘された.早期にCABGを実施する必要から,まず狭窄が比較的穏やかな右内頸動脈起始部の狭窄病変に対してSMART stentを留置し,1週間後に左CEAを施行した.その3週間後にCABGを施行し,周術期合併症はなかった
  • 宝金 清博, 本望 修 医学のあゆみ 212 (6) 569 -574 2005年02月 [査読無し][通常論文]
  • YASUDA Hiroshi, KURODA Satoshi, USHIKOSHI Satoshi, KATO Tsutomu, HOUKIN Kiyohiro, IWASAKI Yoshinobu, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 45 (1) 37 -40 2005年01月15日 [査読無し][通常論文]
     
    A 67-year-old male presented with a rare pseudoaneurysm caused by infection after carotid endarterectomy (CEA) performed for stenosis of the left internal carotid artery (ICA). Wound infection and recurrent bleeding from the operated ICA developed 1 month after surgery. Serial angiography showed that the post-CEA pseudoaneurysm gradually increased in size. The carotid balloon occlusion test revealed that the patient could not tolerate permanent ICA occlusion because of poorly developed collaterals. Direct surgical exposure of the aneurysm was impossible due to tight adhesion of the surrounding tissue, so common carotid to middle cerebral artery bypass using a radial artery graft was performed followed by ligation of the distal common carotid artery. Subsequently, retrograde blood flow from the ICA to the aneurysm was interrupted by embolization of the external carotid artery coil through the facial artery. Combined surgical and endovascular treatment is a therapeutic option for patients with post-CEA pseudoaneurysm, if either direct or endovascular surgery is unfeasible.
  • 上矢状静脈洞の著明な拡張と血栓を伴った全身性エリテマトーデスの1例
    小泉 武, 大久保 由希子, 静川 裕彦, 久原 真, 野中 道夫, 今井 富裕, 千葉 進, 松本 博之, 大坂 美鈴, 宝金 清博 臨床神経学 45 (1) 61 -61 2005年01月 [査読無し][通常論文]
  • 黒田敏, 石川達哉, 志賀哲, 加藤千恵次, 中山若樹, 宝金清博, 玉木長良, 岩崎喜信 日本脳循環代謝学会総会プログラム・抄録集 17th 127 2005年 [査読無し][通常論文]
  • 中山若樹, 黒田敏, 石川達哉, 志賀哲, 宝金清博, 玉木長良, 岩崎喜信 日本脳循環代謝学会総会プログラム・抄録集 17th 130 2005年 [査読無し][通常論文]
  • 三上 毅, 南田 善弘, 八巻 稔明, 馬場 雄大, 宝金 清博 脳外誌 14 (11) 683 -688 2005年 [査読無し][通常論文]
     
    三叉神経痛や顔面痙攣は血管の圧迫状況と神経血管減圧術の手術成績に相関があるといわれている.Fast imaging employing steady-state acquisition (FIESTA)は従来よりも高い空間分解能を持ち, 脳槽内の詳細な解剖を描出させることが可能なsequenceである.われわれは, FIESTAを用いて術前後の評価を行った.対象は三叉神経痛6例, 顔面痙攣14例でありFIESTAとSPGRを用いて術前術後の評価を行った.結果は, 動脈による圧迫例はFIESTAとSPGRいずれにおいても確認されたが, 静脈による圧迫例の2例中1例はSPGRで検出されず, FIESTAで確認された.また術後評価において, prosthesisは全例FIESTAでしか認識できなかった.今回の検討では, FIESTAは静脈描出にも優れ, このような情報を術前に評価できれば, 個々の症例において効果の期待値を予測することも可能であると考えられた.
  • K Kurozumi, K Nakamura, T Tamiya, Y Kawano, K Ishii, M Kobune, S Hirai, H Uchida, K Sasaki, Y Ito, K Kato, O Honmou, K Houkin, Date, I, H Hamada MOLECULAR THERAPY 11 (1) 96 -104 2005年01月 [査読無し][通常論文]
     
    Mesenchymal stem cells (MSC) were reported to ameliorate functional deficits after stroke in rats, with some of this improvement possibly resulting from the action of cytokines secreted by these cells. To enhance such cytokine effects, we previously transfected the telomerized human MSC with the BDNF gene using a fiber-mutant adenovirus vector and reported that such treatment contributed to improved ischemic recovery in a rat transient middle cerebral artery occlusion (MCAO) model. In the present study, we investigated whether other cytokines in addition to BDNF, i.e., GDNF, CNTF, or NT3, might have a similar or greater effect in this model. Rats that received MSC-BDNF (P < 0.05) or MSC-GDNF (P < 0.05) showed significantly more functional recovery as demonstrated by improved behavioral test results and reduced ischemic damage on MRI than did control rats 7 and 14 days following MCAO. On the other hand, rats that received MSC-CNTF or MSC-NT3 showed neither functional recovery nor ischemic damage reduction compared to control rats. Thus, MSC transfected with the BDNF or GDNF gene resulted in improved function and reduced ischemic damage in a rat model of MCAO. These data suggest that gene-modified cell therapy may be a useful approach for the treatment of stroke.
  • 宝金 清博 脳神経外科ジャーナル 14 (4) 248 -248 2005年 [査読無し][通常論文]
  • T Mikami, K Houkin, K Hashi JOURNAL OF NEUROSURGERY 101 (6) 1082 -1083 2004年12月 [査読無し][通常論文]
  • 小柳 泉, 宝金 清博, 今村 博幸, 藤本 真, 飛騨 一利, 岩崎 喜信 神経外傷 27 (1) 4 -6 2004年12月 [査読無し][通常論文]
  • S Oka, O Honmou, Y Akiyama, M Sasaki, K Houkin, K Hashi, JD Kocsis BRAIN RESEARCH 1030 (1) 94 -102 2004年12月 [査読無し][通常論文]
     
    The objective of this study was to establish if neural precursor cells could safely be developed from biopsy of the subventricular zone (SVZ) in the non-human primate (marmoset), and to determine their myelinating potential after autologous transplantation into a demyelinated lesion. Small amounts of tissue were safely collected from the subventricular-subependymal zone of the adult primate brain under ultrasonography without any neurological deficit. Neural precursor cells were isolated and expanded in the presence of mitogen in vitro. The dorsal columns of the adult marmoset spinal cord were demyelinated by X-irradiation and intraspinal injections of ethidium bromide in the center of the radiation field. Cell suspensions of the neural precursors were microinjected through a micropipette into the demyelinated lesion site in the spinal cord. Lesions were histologically examined 3 weeks after transplantation. Light and electron microscopic examination of plastic embedded sections revealed a significant number of myelinating profiles in the transplantation zone; no myelination was observed in control lesions. The myelinated axons had predominantly peripheral patterns of myelination. These results demonstrate that autologous transplantation of neural precursor cells in the adult nonhuman primate can remyelinate demyelinated central nervous system (CNS) axons, thus suggesting the potential utility of such an approach in demyelinating lesions in humans. (C) 2004 Elsevier B.V. All rights reserved.
  • 小柳 泉, 小野 誠司, 宝金 清博 臨床神経生理学 32 (5) 603 -604 2004年10月 [査読無し][通常論文]
  • 神経血管減圧術が有効であった上斜筋ミオキミアの2例
    花井 香織, 橋本 雅人, 小原 裕一郎, 鈴木 康夫, 大塚 賢二, 宝金 清博 神経眼科 21 (増補1) 49 -49 2004年10月 [査読無し][通常論文]
  • K Houkin, N Nakayama, S Kuroda, T Ishikawa, T Nonaka CHILDS NERVOUS SYSTEM 20 (10) 734 -741 2004年10月 [査読無し][通常論文]
     
    Object: The dynamic process of neovascularization seen in patients with moyamoya disease who have received bypass surgery has not been sufficiently elucidated. Magnetic resonance angiography (MRA) is expected to disclose this process since it can be conducted frequently because of its non-invasive nature. In this study, we prospectively investigated postoperative neovascularization using repeated MRA. Methods: We studied 14 pediatric patients ( 23 sides) who have received both direct bypass surgery of the superficial temporal artery (STA) to middle cerebral artery and indirect surgery of encephalo-duro-arterio-myo-synangiosis during the past 3 years. MRA was performed using a 1.5-T machine, image sequences of 512 x 256 resolution, and the time-of-flight method. MRA was prospectively performed preoperatively and repeated 1, 3, and 6 months after surgery. Conclusions: A tendency toward a decrease in moyamoya vessels and the development of the STA was observed 1 month after the surgery. Development of the deep temporal artery and the middle meningeal artery was observed 3 months after the surgery. The progression of stenotic change was apparently accelerated after the surgery. A reciprocal relation between neovascularization artificially induced by bypass surgery and moyamoya vessels, which are supposed to compensate for the stenotic change of the internal carotid artery, was disclosed.
  • Koyanagi, I, H Imamura, S Fujimoto, K Hida, Y Iwasaki, K Houkin, DJ Maiman SURGICAL NEUROLOGY 62 (4) 286 -291 2004年10月 [査読無し][通常論文]
     
    BACKGROUND The size of the spinal canal is a factor that contributes to the neurologic deficits associated with cervical ossification of the posterior longitudinal ligament (OPLL). METHODS Bone-window computed tomography (CT) examinations of the cervical spine in 64 patients with cervical OPLL were reviewed. Forty-two patients underwent surgical treatment (anterior decompression: 16 patients, posterior decompression: 26 patients). The remaining 22 patients were managed conservatively. Selection of the surgical approach, anterior or posterior, was based on the longitudinal extent of cord compression. RESULTS The mean developmental size of the spinal canal in the posterior decompression group (10.7 mm at C4) was significantly smaller than the other 2 groups. The spinal canal was narrowed by OPLL to 2.9 to 10.0 mm. The proportion of the patients showing motor deficits of the lower extremities significantly increased when the sagittal canal diameter was narrowed to less than 8 mm. CONCLUSIONS This study demonstrates critical values of CT-determined spinal canal stenosis. Developmental size of the spinal canal and the residual anterior-posterior canal diameters resulting from OPLL spinal cord compression are important factors influencing clinical management and the neurologic state. (C) 2004 Elsevier Inc. All rights reserved.
  • 外山 賢太郎, 南田 善弘, 三上 毅, 八巻 稔明, 田辺 純嘉, 宝金 清博, 今泉 俊雄 新潟医学会雑誌 118 (10) 555 -555 2004年10月 [査読無し][通常論文]
  • 小柳 泉, 宝金 清博, 八巻 稔明, 野中 雅, 南田 善弘, 三上 毅, 岡 真一 新潟医学会雑誌 118 (10) 558 -558 2004年10月 [査読無し][通常論文]
  • 野中 雅, 宝金 清博 Medicina 41 (9) 1464 -1467 2004年09月 [査読無し][通常論文]
  • 南田 善弘, 宝金 清博 Medicina 41 (9) 1468 -1470 2004年09月 [査読無し][通常論文]
  • K Houkin NEUROLOGIA MEDICO-CHIRURGICA 44 (9) 455 -455 2004年09月 [査読無し][通常論文]
  • M Hashimoto, K Ohtsuka, Y Suzuki, Y Minamida, K Houkin JOURNAL OF NEURO-OPHTHALMOLOGY 24 (3) 237 -239 2004年09月 [査読無し][通常論文]
     
    A 49-year-old man had left superior oblique myokymia for eight years. Magnetic resonance images with enhanced spoiled gradient recalled acquisition in the steady state (SPGR) and flow imaging using steady acquisition (FIESTA) disclosed a branch of the superior cerebellar artery lying on the root exit zone of the left trochlear nerve. Posterior fossa craniotomy confirmed the imaging findings. A Teflon pad was placed between the compressing artery and the trochlear nerve. The patient's superior oblique myokymia has completely resolved with a one-year follow-up. Only one such case has been previously reported. This is the first report to display the imaging findings.
  • 野中 雅, 岡 真一, 宮田 圭, 三上 毅, 宝金 清博, 鹿野 恒 脳卒中の外科 32 (5) 376 -380 2004年09月 [査読無し][通常論文]
     
    37歳女.妊娠25週の切迫早産のため子宮収縮抑制剤塩酸リトドリンで点滴治療中であった.くも膜下出血をきたしたため開頭術を行い,血腫の吸引洗浄と脳動脈瘤のクリッピングを行った.血腫は非常に硬かったため十分に排除できず,術後,ウロキナーゼによる脳室-脳槽還流を行った.また,塩酸ファスジルの静脈内投与を行ったが意識状態の低下と運動性失語症を来し,脳血管撮影所見にて著明な脳血管攣縮を認めた.通常の2倍量の塩酸ファスジルの動注を行ったが十分な血管拡張を得られず,作用機序の異なる血管拡張剤ミルリノンの動注を追加したところ著明に改善した.経過中,塩酸リトドリンの投与は継続し妊娠36週で帝王切開にて無事に出産した.以上より,妊産婦のくも膜下出血に対して,積極的な外科的治療と塩酸ファスジルとミルリノンの併用が有効であった
  • K Satoshi, H Kiyohiro, Tatsuya, I, Yoshinobu, I NEUROSURGERY 55 (2) 452 -452 2004年08月 [査読無し][通常論文]
  • S Tsushima, M Kai, K Yamada, S Imai, K Houkin, H Kanoh, F Sakane JOURNAL OF BIOLOGICAL CHEMISTRY 279 (27) 28603 -28613 2004年07月 [査読無し][通常論文]
     
    Nine diacylglycerol kinase (DGK) isozymes have been identified. However, our knowledge of their individual functions is still limited. Here, we demonstrate the role of DGKgamma in regulating Rac1-governed cell morphology. We found that the expression of kinase-dead DGKgamma, which acts as a dominant-negative mutant, and inhibition of endogenous DGKgamma activity with R59949 induced lamellipodium and membrane ruffle formation in NIH3T3 fibroblasts in the absence of growth factor stimulation. Reciprocally, lamellipodium formation induced by platelet-derived growth factor was significantly inhibited upon expression of constitutively active DGKgamma. Moreover, the constitutively active DGKgamma mutant suppressed integrin-mediated cell spreading. These effects are isoform-specific because, in the same experiments, none of the corresponding mutants of DGKalpha and DGKbeta, closely related isoforms, affected cell morphology. These results suggest that DGKgamma specifically participates in the Rac1-mediated signaling pathway leading to cytoskeletal reorganization. In support of this, DGKgamma co-localized with dominant-active Rac1 especially in lamellipodia. Moreover, we found that endogenous DGKgamma was physically associated with cellular Rac1. Dominant-negative Rac1 expression blocked the lamellipodium formation induced by kinase-dead DGKgamma, indicating that DGKgamma acts upstream of Rac1. This model is supported by studies demonstrating that kinase-dead DGKgamma selectively activated Rac1, but not Cdc42. Taken together, these results strongly suggest that DGKgamma functions through its catalytic action as an upstream suppressor of Rac1 and, consequently, lamellipodium/ruffle formation.
  • 本望 修, 宝金 清博 Neurological Surgery 32 (6) 571 -577 2004年06月 [査読無し][通常論文]
  • 黒田 敏, 牛越 聡, 新谷 好正, 長内 俊也, 石川 達哉, 瀧川 修吾, 宝金 清博, 岩崎 喜信 Neurological Surgery 32 (6) 645 -650 2004年06月 [査読無し][通常論文]
     
    症例1は37歳女で,両側内頸動脈瘤が発見された.両側内頸動脈瘤-眼動脈分岐部動脈瘤と診断した.右内頸動脈瘤でcoil compactionによる残存頸部の拡大が認められた.橈骨動脈グラフトを採取して外頸動脈-中大脳動脈バイパス術を施行した.右後頭葉に脳梗塞が出現し,左同名半盲を後遺して自宅退院した.症例2は55歳女で,血小板減少症であった.突然の頭痛が出現し,くも膜下出血と診断された.コイル塞栓術を実施し,破裂部位は閉塞されていると判断して手技を終了した.その後,クリッピング術を施行し,術後2年間,動脈瘤の再発はない.症例3は54歳男で,突然の頭痛,意識障害が発症し,CT上ではびまん性くも膜下出血を認めた.コイル塞栓術を実施し,腰椎ドレナージを施行し,意識障害は徐々に改善した.その後開頭術を施行し,脳動脈瘤の根治が確認され退院した
  • IA Awad, DL Barrow, K Houkin NEUROLOGIA MEDICO-CHIRURGICA 44 (6) 286 -287 2004年06月 [査読無し][通常論文]
  • ZY Tong, T Yamaki, K Harada, K Houkin MAGNETIC RESONANCE IMAGING 22 (5) 735 -742 2004年06月 [査読無し][通常論文]
     
    Metabolite concentrations in normal adult brains and in gliomas were quantitatively analyzed by in vivo proton magnetic resonance spectroscopy (MRS) using the fully relaxed water signal as an internal standard. Between January 1998 and October 2001, 28 healthy volunteers and 18 patients with gliomas were examined by in vivo proton MRS. Single-voxel spectra were acquired using the point-resolved spectroscopic (PRESS) pulse sequence with a 1.5 T scanner (TR/TE/Ave = 3000 ms/30 ms/64). The calculated concentrations of N-acetyl-aspartate (NAA), creatine (Cre), choline (Cho), and water(H2O) in the normal hemispheric white matter were 23.59 +/- 2.62 mM (mean +/- SD), 13.06 +/- 1.8 mM, 4.28 +/- 0.8 mM, and,47280.96 +/- 5414.85 mM, respectively. The metabolite concentrations were not necessarily uniform in different parts of the brain. The concentrations of NAA and Cre decreased in all gliomas (p < 0.001). The NAA/Cho and NAA/H2O ratios can distinguish the normal brain from gliomas and low-grade from high-grade astrocytoma (p < 0.001). The concentration of taurine (Tau) in medulloblastomas was 29.64 +/- 5.76 mM. This is the first quantitative analysis of Tau in medulloblastoma in vivo and confirms earlier in vitro findings. (C) 2004 Elsevier Inc. All rights reserved.
  • ZY Tong, T Yamaki, K Harada, K Houkin MAGNETIC RESONANCE IMAGING 22 (5) 735 -742 2004年06月 [査読無し][通常論文]
     
    Metabolite concentrations in normal adult brains and in gliomas were quantitatively analyzed by in vivo proton magnetic resonance spectroscopy (MRS) using the fully relaxed water signal as an internal standard. Between January 1998 and October 2001, 28 healthy volunteers and 18 patients with gliomas were examined by in vivo proton MRS. Single-voxel spectra were acquired using the point-resolved spectroscopic (PRESS) pulse sequence with a 1.5 T scanner (TR/TE/Ave = 3000 ms/30 ms/64). The calculated concentrations of N-acetyl-aspartate (NAA), creatine (Cre), choline (Cho), and water(H2O) in the normal hemispheric white matter were 23.59 +/- 2.62 mM (mean +/- SD), 13.06 +/- 1.8 mM, 4.28 +/- 0.8 mM, and,47280.96 +/- 5414.85 mM, respectively. The metabolite concentrations were not necessarily uniform in different parts of the brain. The concentrations of NAA and Cre decreased in all gliomas (p < 0.001). The NAA/Cho and NAA/H2O ratios can distinguish the normal brain from gliomas and low-grade from high-grade astrocytoma (p < 0.001). The concentration of taurine (Tau) in medulloblastomas was 29.64 +/- 5.76 mM. This is the first quantitative analysis of Tau in medulloblastoma in vivo and confirms earlier in vitro findings. (C) 2004 Elsevier Inc. All rights reserved.
  • Osamu Honmou, Kiyohiro Houkin Neurological Surgery 32 (6) 571 -577 2004年06月 [査読無し][通常論文]
  • K Kurozumi, K Nakamura, T Tamiya, Y Kawano, M Kobune, S Hirai, H Uchida, K Sasaki, Y Ito, K Kato, O Honmou, K Houkin, Date, I, H Hamada MOLECULAR THERAPY 9 (5) 766 -766 2004年05月 [査読無し][通常論文]
  • K Kurozumi, K Nakamura, T Ichikawa, Y Ono, T Tamiya, Y Ito, O Honmou, K Houkin, H Hamada, Date, I MOLECULAR THERAPY 9 S20 -S20 2004年05月 [査読無し][通常論文]
  • S Kuroda, K Houkin, T Ishikawa, N Nakayama, J Ikeda, N Ishii, H Kamiyama, Y Iwasaki CHILDS NERVOUS SYSTEM 20 (5) 302 -308 2004年05月 [査読無し][通常論文]
     
    Objects. The aim of this study was to clarify predictors for poor intellectual outcome in pediatric moyamoya disease. Methods. Fifty-two pediatric patients were included. Clinical diagnosis was transient ischemic attacks (TIA) in 35 and completed stroke in 17. Ten patients underwent indirect synangiosis through "small craniotomy," whereas the other 42 underwent superficial temporal artery (STA)-middle cerebral artery (MCA) anastomosis and indirect synangiosis through "large craniotomy." Full-scale IQ (FSIQ) was measured using the Wechsler intelligence scale for children (WISC) after surgery. Multivariate logistic regression models were applied to test the effect of clinical factors on intellectual outcome. Results and conclusion. Eight patients revealed mentally impaired status (FSIQ<70). Multivariate analysis revealed that completed stroke and "small craniotomy" surgery were significantly associated with poor intellectual outcome. Odds ratios of each factor were 33.4 (95% CI, 2.4-474) and 19.6 (95% CI, 1.8-215) respectively. Early diagnosis and the revascularization procedure over as wide an area as possible may be essential to improve their intellectual outcome.
  • S Iihoshi, O Honmou, K Houkin, K Hashi, JD Kocsis BRAIN RESEARCH 1007 (1-2) 1 -9 2004年05月 [査読無し][通常論文]
     
    The primary objective of this study was to test the hypothesis that intravenous administration of autologous bone marrow cells could improve functional recovery after middle cerebral artery occlusion (MCAO) for 45 min in the rat and to determine specific time windows for efficacy. Mononuclear cells from autologous bone marrow were transfected with the LacZ reporter gene, and injected intravenously into rats at 3-72 h after induction of MCAO. Histological analysis of the ischemic lesion at 14 days after transplantation revealed reduced ischemic lesion volume. Lesion volume was 250 +/- 45 mm(3) (n = 6) after MCAO without cell transplantation. Lesions were minimally detected by absence of 2,3,5-triphenyltetrazolium chloride (TTC) staining when bone marrow cells were infused 3 h after lesion induction. Lesions were clearly detected beginning with the 6-h postlesion group and became progressively larger at 12, 24 and 72 h (80 +/- 25, 140 +/- 18, and 180 +/- 22 mm(3), respectively; n=6 for each group). Transplanted LacZ(+) bone marrow cells accumulated extensively in and around the ischemic lesions, and immunohistochemistry suggests some neuronal and glial lineage differentiation. Behavioral testing (Morris water maze and Treadmill stress test) indicated greater functional recovery in the treated group. These findings suggest that early intervention with intravenous administration of autologous mononuclear cells from bone marrow can reduce lesion size in the MCAO model in the rat, and improve functional outcome. (C) 2004 Elsevier B.V. All rights reserved.
  • 三上 毅, 高橋 明, 宝金 清博 Neurosonology 17 (1) 10 -13 2004年05月 [査読無し][通常論文]
     
    椎骨動脈起始部病変検出における超音波検査の限界について検討した.対象は,最近2年間に脳血管撮影と超音波検査で4 vessels studyを行った60例(男性35例,女性25例・平均年齢67.4歳)で,椎骨動脈の形態学的診断・評価を行った.椎骨動脈の検出率はcolor Doppler imaging(CDI), power Doppler imaging(PDI)を用いて95%で,右側は56例(93%),左側は58例(97%)であった.B-modeで血管壁が確認できたのは61%で,右側は70%,左側52%であった.脳血管撮影と比較した椎骨動脈起始部の診断ではfalse positive 1,false negative 8で,感度93.3%,特異度99.2%であった.脳血管撮影で50%以上の狭窄性病変を認めたのは右側2例,左側3例であったが,超音波検査で検出できたのは左側2例のみで,プラークの検出はできたが範囲や性状評価までに至らず,いずれもdiastolic flowが欠如している所見であった
  • 小柳 泉, 宝金 清博, 南田 善弘, 岩崎 喜信, 飛騨 一利, 関 俊隆, 井須 豊彦 小児の脳神経 29 (2) 159 -159 2004年04月 [査読無し][通常論文]
  • 標準治療と最新治療 メリット・デメリット 脳血管攣縮
    野中 雅, 宝金 清博 Clinical Neuroscience 22 (4) 484 -485 2004年04月 [査読無し][通常論文]
  • S Kuroda, K Houkin, T Ishikawa, Y Iwasaki JOURNAL OF NEUROSURGERY 100 (4) 791 -792 2004年04月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 13 (4) 323 -323 2004年04月 [査読無し][通常論文]
  • 宝金 清博 脳卒中 26 (1) 171 -171 2004年03月 [査読無し][通常論文]
  • 宝金 清博, 野中 雅 脳卒中 26 (1) 216 -216 2004年03月 [査読無し][通常論文]
  • 宝金 清博, 野中 雅 脳卒中 26 (1) 279 -279 2004年03月 [査読無し][通常論文]
  • 脊髄・末梢神経の手術 スタンダードとバリエーション 急性頸髄損傷の手術
    小柳 泉, 宝金 清博, 今村 博幸, 飛騨 一利, 岩崎 喜信 日本神経外傷学会プログラム・抄録集 27回 53 -53 2004年03月 [査読無し][通常論文]
  • 急性頸髄損傷を示した頸椎後縦靱帯骨化症の臨床的検討
    小柳 泉, 宝金 清博, 今村 博幸, 飛騨 一利, 岩崎 喜信 日本神経外傷学会プログラム・抄録集 27回 107 -107 2004年03月 [査読無し][通常論文]
  • S Kuroda, K Furukawa, T Shiga, S Ushikoshi, C Katoh, T Aoki, T Ishikawa, K Houkin, N Tamaki, Y Iwasaki NEUROSURGERY 54 (3) 585 -591 2004年03月 [査読無し][通常論文]
     
    OBJECTIVE: Retrograde drainage into the cortical veins results in poor outcome in patients with an intracranial dural arteriovenous fistula. However, the pathophysiological features of dural arteriovenous fistulae remain obscure. This study aimed to clarify hemodynamic and metabolic conditions in these patients using positron emission tomography. METHODS: This study included eight patients with an intracranial dural arteriovenous fistula. All patients had cortical venous reflux, as demonstrated by angiography. Regional cerebral blood flow (rCBF), regional cerebral blood volume (rCBV), regional cerebral metabolic rate for oxygen, and regional oxygen extraction fraction (rOEF) were measured before and after surgical or endovascular treatment using positron emission tomography. RESULTS: Pretreatment positron emission tomographic studies revealed that all patients had abnormal hemodynamic and metabolic parameters in the area that was drained by the involved cortical veins. A severe increase in rCBV was noted in seven of the eight patients. A significant decrease in rCBF also was observed in all eight patients. A negative correlation was observed between rCBF and rCBV. Three patients had an elevated rOEF. Oxygen metabolism was impaired in seven patients. All patients underwent successful treatment. Follow-up studies demonstrated significant improvements in rCBF, rCBV, and regional cerebral metabolic rate for oxygen. The improvement in regional cerebral metabolic rate for oxygen varied among the patients. Normalization of rOEF also was confirmed in three patients who had increased rOEF before the treatment. CONCLUSION: The present results suggest that hemodynamic and metabolic characteristics vary widely among patients with cortical venous reflux. It is essential to precisely evaluate hemodynamic and metabolic conditions to predict their outcomes and therapeutic effects.
  • Y Takamura, H Ikeda, T Kanaseki, M Toyota, T Tokino, K Imai, K Houkin, N Sato GLIA 45 (4) 392 -405 2004年03月 [査読無し][通常論文]
     
    We first classified 12 malignant glioma cell lines into three different groups (types 1-3) with respect to major histocompatibility complex (MHC) class II expression and analyzed each group based on the different expression status of the class II transactivator (CIITA) gene. Glioma type 1 (2 of 12) showed constitutive expression of all class II molecules that might be mediated by activation of B cell-specific CIITA promoter III. Glioma type 2 represented the major phenotype (66.7 %) of malignant glioma cell lines, and MHC class II expression was induced by interferon-gamma (IFN-gamma) in this phenotype. Analysis of glioma tissue samples revealed that CIITA promoter IV was detected in 9 of 11 patients (81.8%); however, promoter III was only in two (18.2%). Moreover, cultured glioma cells obtained from a fresh tumor sample upregulated expression of CIITA and class II molecules in the presence of IFN-gamma, strongly suggesting that glioma type 2 might be predominant in glioma tissues. Glioma type 3 (2 of 12) showed CIITA transcripts but loss of MHC class II expression even in the presence of IFN-gamma. In addition, we determined that the constitutive MHC class II expression in the glioma cell lines (type 1) was the result of transcriptional activation of the CIITA gene. This phenomenon was mediated by global histone acetylation over 6 kb upstream from the transcriptional start site of CIITA promoter III. Moreover, stable transfection of CIITA promoter IV as well as promoter III into MHC class Il inducible cell lines restored the constitutive expression of all class II molecules. These studies lay the foundation to understand the molecular basis for the expression of class Il molecules in gliomas. (C) 2003 Wiley-Liss, Inc.
  • 南田 善弘, 宝金 清博 Brain Nursing 20 (3) 268 -274 2004年03月 [査読無し][通常論文]
  • 黒田 敏, 七戸 秀夫, 小林 徹, 吉本 哲之, 鐙谷 武雄, 石川 達哉, 多田 光宏, 中井 章人, 宝金 清博, 岩崎 喜信 Pharma Medica 22 (2) 130 -130 2004年02月 [査読無し][通常論文]
  • 宝金 清博, 南田 善弘, 野中 雅, 三上 毅, 小柳 泉 Neurological Surgery 32 (2) 111 -119 2004年02月 [査読無し][通常論文]
     
    札幌医科大学脳神経外科における過去5年間のインシデンス発生率は,入院患者数に対して1.09%,過去1年間の合併症発生率は8.94%であった.この値は過去に報告された米国での脳神経外科でのリスクとほぼ同等であった.またインシデンス,合併症の70%以上は,手術に関連したものであった.このように脳神経外科はリスクの高い専門科である.したがって,リスクマネジメントは個々の医療単位で行っていかなければならないが,データの共有がなければ,効率も悪く効果も不十分であり,組織的に行っていくことが安全で確度の高い治療に直結すると考えられる
  • K Houkin, Y Minamida, T Nonaka, T Mikami, Koyanagi, I NEUROLOGICAL SURGERY 32 (2) 111 -119 2004年02月 [査読無し][通常論文]
  • K Kurozumi, K Nakamura, T Tamiya, Y Kawano, M Kobune, S Hirai, H Uchida, K Sasaki, Y Ito, K Kato, O Honmou, K Houkin, Date, I, H Hamada MOLECULAR THERAPY 9 (2) 189 -197 2004年02月 [査読無し][通常論文]
     
    Examination of the clinical therapeutic efficacy of using bone marrow stromal cells, including mesenchymal stem cells (MSC), has recently been the focus of much investigation. MSC were reported to ameliorate functional deficits after stroke in rats, with some of this improvement possibly resulting from the action of cytokines secreted by these cells. To enhance such cytokine effects, we transfected telomerized human MSC with the BDNF gene using a fiber-mutant F/RGD adenovirus vector and investigated whether these cells contributed to improved functional recovery in a rat transient middle cerebral artery occlusion (MCAO) model. BDNF production by MSC-BDNF cells was 23-fold greater than that seen in uninfected MSC. Rats that received MSC-BDNF showed significantly more functional recovery than did control rats following MCAO. Specifically, MRI analysis revealed that the rats in the MSC-BDNF group exhibited more significant recovery from ischemia after 7 and 14 days. The number of TUNEL-positive cells in the ischemic boundary zone was significantly smaller in animals treated with MSC-BDNF compared to animals in the control group. These data suggest that MSC transfected with the BDNF gene may be useful in the treatment of cerebral ischemia and may represent a new strategy for the treatment of stroke.
  • 難波 理奈, 黒田 敏, 石川 達哉, 多田 光宏, 宝金 清博, 岩崎 喜信 Neurological Surgery 32 (1) 7 -16 2004年01月 [査読無し][通常論文]
  • T Mikami, A Takahashi, K Hashi, S Gasa, K Houkin JOURNAL OF NEUROSURGERY 100 (1) 133 -138 2004年01月 [査読無し][通常論文]
     
    The aim of this study was to measure objectively the adherence of burned tissue to bipolar forceps to evaluate the coagulation performance of forceps made of different types of metals. Coagulation performance of bipolar forceps made of gold, titanium, and stainless steel was determined by comparing the amount of protein in the adhered coagulum on the tips. The amount of adhered coagulum was significantly less on the gold-plated bipolar forceps than on those made of the other two materials. The ease with which coagulum could be removed was compared using the cleaning cycle of an ultrasonic rinsing device. This ease of removal was also significant with the gold-plated forceps. Electron microscopy observations of the surface of the forceps tips revealed a significant difference in roughness among these materials, and there were also significant differences in wetting tensions. Measuring adherence based on three different types of roughness and wetting tensions of forceps made from the same metal (titanium) also demonstrated a significant difference in the cleaning cycle. Histological examination of an artery coagulated with the goldplated bipolar forceps showed that the structure had been completely collapsed without destruction of the layers, whereas arteries coagulated with the other materials revealed severely damaged structures. Adherence to bipolar forceps was dependent on both the material in the tips and the roughness of this material. The gold-plated bipolar forceps demonstrated the best performance.
  • 鹿野 恒, 寶金 清博, 原田 邦明 新潟医学会雑誌 = 新潟医学会雑誌 118 (1) 76 -76 2004年01月 [査読無し][通常論文]
  • 難波 理奈, 黒田 敏, 竹田 誠, 七戸 秀夫, 中山 若樹, 石川 達哉, 宝金 清博, 岩崎 喜信 Neurological Surgery 31 (12) 1291 -1295 2003年12月 [査読無し][通常論文]
     
    過去10年間に著者らが経験した成人無症候性もやもや病10例(男性4例,女性6例・平均年46.2歳)の臨床像,予後について検討した.脳MRIでは脳梗塞を3例に認め,梗塞部位はMCA-ACAあるいはMCA-PCA境界域,尾状核であった.DSAでは,全例病変は両側に存在し,鈴木の病期分類は30歳代で1〜3期が多く,50歳以降では4〜6期の例が多い傾向にあった.SPECTやPETによる脳循環代謝パラメータでは,内頸動脈領域の脳虚血を4例に認め,うち2例は脳梗塞を有した.平均4.1年間の経過観察中,1例でもやもや病の病期が進行し,脳梗塞を発症したが,その他の9例は経過観察期間中に脳虚血発作を呈した例はなく,うち1例は診断2年目に妊娠し,帝王切開にて無事出産した
  • R Nanba, S Kuroda, M Takeda, H Shichinohe, N Nakayama, T Ishikawa, K Houkin, Y Iwasaki NEUROLOGICAL SURGERY 31 (12) 1291 -1295 2003年12月 [査読無し][通常論文]
     
    Recent development of non-invasive diagnostic technology, such as magnetic resonance imaging (MRI) and angiography (MRA), is believed to have made possible on increase in the diagnoses of asymptomatic moyamoya disease. However, no criteria have been established for the management of such cases. The present study aimed to clarify the natural history of asymptomatic moyamoya disease retrospectively. Ten patients were included in this study. None of them had experienced any episode due to moyamoya disease and were only incidentally diagnosed as having moyamoya disease. There were 4 males and 6 females. Their ages ranged from 30 to 67 years, with the mean age of 46.2. Cerebral angiography showed there was the tendency of disease progression in elder patients. MRI detected cerebral infarction in 3 of 10 patients (30%). Hemodynamic ischemia, such as impaired reactivity to acetazolamide and/or elevated oxygen extraction fraction, was observed in 4 of 10 patients. Only one patient under-went surgical revascularization. Antiplatelet or anticonvulsant medication was administered in 5 of 10 patients. The mean follow-up period was 4.1 years, ranging from 0.5 to 13 years. During follow-up periods, the moyamoya lesion markedly progressed and caused cerebral infarction in one patient. However, neither ischemic nor hemorrhagic stroke occurred in the other 9 patients. Multi-center nation-wide study should be planned to clarify the natural course of asymptomatic moyamoya disease and establish the management guidelines for patients with asymptomatic moyamoya disease.
  • F Qiao, S Kuroda, K Kamada, K Houkin, Y Iwasaki CHILDS NERVOUS SYSTEM 19 (10-11) 760 -764 2003年11月 [査読無し][通常論文]
     
    Objects. The "re-build up" phenomenon is a pathognomonic finding induced after hyperventilation in pediatric moyamoya disease, and is closely related to ischemic attacks. However, its precise mechanism remains unknown. Methods. In this study, MEG, MRI, and SPECT were used to clarify its mechanism in 4 children with moyamoya disease. Cerebral blood flow before and after acetazolamide administration was measured. Spontaneous magnetic brain activity was measured using a whole-head MEG system. The quantified dipole distribution was shown on 3D-MRI. Results and conclusion. The original current dipoles of the "re-build up" slow waves were predominantly located in the deep cortical sulci in the area with impaired reactivity to acetazolamide. The results suggested that the re-build up waves derive from the deep cortical sulci due to the reduction in the cerebral perfusion reserve.
  • M Inoue, O Honmou, S Oka, K Houkin, K Hashi, JD Kocsis GLIA 44 (2) 111 -118 2003年11月 [査読有り][通常論文]
     
    The remyelinating potential of autologous bone marrow cells was studied after direct injection and following intravenous injection into rats with a demyelinated lesion in the spinal cord. Both focal and intravenous injections of acutely isolated mononuclear bone marrow cell fractions resulted in varying degrees of remyelination. Suspensions of bone marrow cells collected from the same rat were delivered at varied concentrations (10(2) to 10(5) for direct injection and 10(4) to 10(7) for i.v. injections). The lesions were examined histologically 3 weeks after transplantation. Light microscopic examination revealed remyelination in the dorsal funiculus with both injection protocols, but the extent of remyelination was proportional to the number of injected cells. To attain the same relative density of remyelination achieved by direct injection, intravenous administration of cells required delivery of substantially more cells (two orders of magnitude). However, the availability of autologous bone marrow cells in large number and the potential for systemically delivering cells to target lesion areas without neurosurgical intervention suggest the potential utility of intravenous cell delivery as a prospective therapeutic approach in demyelinating disease. (C) 2003 Wiley-Liss, Inc.
  • 黒田 敏, 難波 理奈, 石川 達哉, 宝金 清博, 上山 博康, 岩崎 喜信 Neurological Surgery 31 (10) 1073 -1078 2003年10月 [査読無し][通常論文]
     
    1982〜2000年に入院したもやもや病55例を対象とし,就学以前に発症した乳幼児もやもや病の臨床像を,就学以降に発症した学童もやもや病と比較した.就学以前に発症した乳幼児もやもや病は32例(58.2%)で,就学以降に発症した学童もやもや病は23例であった.乳幼児もやもや病は脳梗塞による発症が多く,画像上も脳梗塞,特に皮質枝領域の脳梗塞を高率に有していた.乳幼児もやもや病は後大脳動脈の狭窄性病変を高率に合併し,脳血流量も低値の傾向を示した.脳血行再建術の効果や合併症には差はみられなかった.乳幼児もやもや病では,術後の知能予後は不良で,脳梗塞を有している症例で顕著であった
  • S Kuroda, R Nanba, T Ishikawa, K Houkin, H Kamiyama, Y Iwasaki NEUROLOGICAL SURGERY 31 (10) 1073 -1078 2003年10月 [査読無し][通常論文]
     
    Purpose: The present study aimed to clarify clinical manifestations of infantile moyamoya disease by comparing them with those of schoolchild moyamoya disease. Subjects and Methods: This study included a total of 55 patients who were diagnosed as moyamoya disease on cerebral angiography. Their medical. records were precisely evaluated. CT or MRI was performed to examine the location of cerebral infarction in all subjects.. Cerebral blood flow was measured in 33 patients before surgery, using the 133-xenon inhalation method and single photon emission computed tomography (SPECT). Postoperative full-scale IQ (FSIQ) was also examined. Results: Of 55 patients, onset age was < 6 yr in 32 (infantile group) and 6 yr in 23 (schoolchild group). Completed stroke was significantly more frequent in the infantile group than in schoolchild group (p = 0.0014). Incidence of cerebral infarct was also higher in the infantile group than in the schoolchild group (p = 0.0023). PCA stenosis/occlusion was observed in 14 of 32 infantile patients, but only in 4 of 23 schoolchild patients (p = 0.0474). Mean cerebral blood flow value was significantly lower than control value in the infantile group, but not in the schoolchild group. The effect of bypass surgery on cerebral ischemic attack was satisfactory in both groups. However, the postoperative FSIQ value was significantly lower in the infantile group than in the schoolchild group (p = 0.0452). Conclusions: The present study showed that infantile moyamoya disease involves a higher risk for cerebral infarct and completed stroke due to its more advanced stage, and that earlier diagnosis and treatment should be performed to improve the mental prognosis of patients.
  • 宝金 清博 Therapeutic Research 24 (9) 1761 -1763 2003年09月 [査読無し][通常論文]
  • 庄内 孝春, 秋葉 英成, 晴山 雅人, 宝金 清博 Therapeutic Research 24 (9) 1768 -1773 2003年09月 [査読無し][通常論文]
  • 佐々木 祐典, 本望 修, 宝金 清博 Surgery Frontier 10 (3) 267 -272 2003年09月 [査読無し][通常論文]
     
    既に神経幹細胞は,種々の神経疾患モデルへの移植実験で,良好な生着・遊走・分化能を示すことが報告されている.又,骨髄中に存在し,造血系以外に分化しうる臓器非特異的幹細胞にも同様に注目が集まっており,この細胞を用いた神経再生の研究も進行している.ドナー細胞の確保という観点からは,骨髄に存在する幹細胞を用いた細胞移植療法は,問題が少なく,臨床応用に向けて,非常に有望と考えられる.そこで,これらの細胞を用いた細胞移植療法による神経再生研究の現状について概説した
  • T Murakami, F Sakane, S Imai, K Houkin, H Kanoh JOURNAL OF BIOLOGICAL CHEMISTRY 278 (36) 34364 -34372 2003年09月 [査読無し][通常論文]
     
    Diacylglycerol kinase (DGK) participates in regulating the intracellular concentrations of two bioactive lipids, diacylglycerol and phosphatidic acid. DGKeta (eta1, 128 kDa) is a type II isozyme containing a pleckstrin homology domain at the amino terminus. Here we identified another DGKeta isoform (eta2, 135 kDa) that shared the same sequence with DGKeta1 except for a sterile alpha motif (SAM) domain added at the carboxyl terminus. The DGKeta1 mRNA was ubiquitously distributed in various tissues, whereas the DGKeta2 mRNA was detected only in testis, kidney, and colon. The expression of DGKeta2 was suppressed by glucocorticoid in contrast to the marked induction of DGKeta1. DGKeta2 was shown to form through its SAM domain homo-oligomers as well as hetero-oligomers with other SAM-containing DGKs (delta1 and delta2). Interestingly, DGKeta1 and DGKeta2 were rapidly translocated from the cytoplasm to endosomes in response to stress stimuli. In this case, DGKeta1 was rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas DGKeta2 exhibited sustained endosomal association. The experiments using DGKeta mutants suggested that the oligomerization of DGKeta2 mediated by its SAM domain was largely responsible for its sustained endosomal localization. Similarly, the oligomerization of DGKeta2 was suggested to result in negative regulation of its catalytic activity. Taken together, alternative splicing of the human DGKeta gene generates at least two isoforms with distinct biochemical and cell biological properties responding to different cellular metabolic requirements.
  • M Kobune, Y Kawano, Y Ito, H Chiba, K Nakamura, H Tsuda, K Sasaki, H Dehari, H Uchida, O Honmou, S Takahashi, A Bizen, R Takimoto, T Matsunaga, J Kato, K Kato, K Houkin, Y Niitsu, H Hamada EXPERIMENTAL HEMATOLOGY 31 (8) 715 -722 2003年08月 [査読無し][通常論文]
     
    Objective. To compare the hematopoietic support provided by telomerized human mesenchymal stem cells (MSCs) and telomerized MSC-derived stromal cells. Methods. We transfected the human telomerase catalytic subunit (hTERT) gene into primary MSCs to establish hTERT-transduced MSCs (hTERT-MSCs). Stromal induction of hTERT-MSCs was performed by replacing the culture medium with Dexter-type culture medium. Hematopoietic support was examined by coculture with cord blood CD34(+) cells. Results. The hTERT-MSCs were morphologically identical with the primary MSCs and expressed surface antigens including CD105, CD73, and CD166. hTERT-MSCs showed a similar doubling time as primary MSCs and continued to proliferate to over 80 population doublings (PD), although the primary MSCs underwent crisis in vitro at 16 PD. The osteogenic, chondrogenic, adipogenic, neurogenic, and stromal differentiation potential of hTERT-MSCs were maintained up to at least 40 PD. The degree of expansion of CD34+ cells and total number of colony-forming units in culture (CFU-C) upon 12-day coculture with the hTERT-MSC-derived stromal cells were nearly the same as those upon 12-day coculture with hTERT-MSCs (CD34, 33.0-fold +/- 2.8-fold vs 36.1-fold +/- 1.7-fold of the initial cell number; CFUs, 344.4-fold +/- 62.5-fold vs 239.3-fold +/- 87.0-fold; CFU-mix, 368.4-fold +/- 113.7-fold vs 341.3-fold +/- 234.3-fold). However, on day 18 of coculture, the number of cobblestone areas (CA) observed beneath the stromal cells was 15 times higher than that beneath hTERT-MSCs (CA, 146.9 +/- 54.6 vs 9.4 +/- 8.1, p < 0.01). Conclusion. Stromal induction of hTERT-MSCs exclusively enhanced the support of CA formation provided by hTERT-MSCs. Our human hTERT-MSCs will be useful for elucidating the mechanism of the formation of CAs. (C) 2003 International Society for Experimental Hematology. Published by Elsevier Inc.
  • H Momota, S Ichimiya, N Kondo, T Kikuchi, T Torigoe, T Yamaki, K Houkin, N Sato INTERNATIONAL JOURNAL OF ONCOLOGY 23 (2) 311 -315 2003年08月 [査読無し][通常論文]
     
    Glioblastoma is a life-threatening tumor in the human brain despite the fact that radio-chemotherapy inducing DNA damage has been improved in the last decade. Various studies focusing on the enhancement of the susceptibility of glioblastoma cells to DNA damage have been reported, which are aimed at more efficient treatment for the tumor. In this study, we show that radioresistant T98G glioblastoma cells can develop sensitivity to DNA damage induced by irradiation and etoposide as a result of the introduction of a DNA repair-associated historic, H2AX. Interestingly, when H2AX-transformed T98G cells were irradiated, Brca1 and Nbs1 were readily recruited in DNA double-strand break (DSB) foci and showed the G2/M-phase arrest of the cell cycle. Moreover, up-regulation of Brcal was observed in H2AX-T98G cells after exposure to irradiation. Together with the evidence that H2AX transfection does not affect growth activities of non-tumor cells under genotoxic stimuli, this suggests that H2AX gene transfer would provide a new modality for radio-chemotherapy for glioblastomas, probably through overcoming the instability of the genome, and that Brca1 and Nbs1 might be crucial in this methodology.
  • 【これだけは知っておきたい臨床医の画像診断】頭部 脳血管障害 脳血管奇形
    米増 保之, 宝金 清博 臨床医 29 (増刊) 706 -707 2003年06月 [査読無し][通常論文]
  • 【これだけは知っておきたい臨床医の画像診断】頭部 脳血管障害 モヤモヤ病
    宝金 清博 臨床医 29 (増刊) 710 -711 2003年06月 [査読無し][通常論文]
  • 二分脊椎症の診断・治療及び予防システムに関する研究 二分脊椎症における遅発性神経症状の危険因子ならびに治療指針に関する研究 腰仙部脊髄脂肪腫の手術治療の合併症と機能予後
    岩崎 喜信, 飛騨 一利, 関 俊隆, 石井 伸明, 小柳 泉, 宝金 清博 厚生労働省精神・神経疾患研究委託費研究報告集 平成14年度 251 -251 2003年06月 [査読無し][通常論文]
  • 金 相年, 鰐渕 昌彦, 南田 善弘, 八巻 稔明, 田邊 純嘉, 宝金 清博 Neurological Surgery 31 (6) 677 -682 2003年06月 [査読無し][通常論文]
     
    66歳男.前額部の腫脹・疼痛,鼻出血,流涙,眼脂を主訴とした.脳浸潤を伴うsinonasal carcinomaと診断し,手術を優先させた.腫瘍除去後,遊離大腿筋膜弁と遊離腹直筋皮弁を使用し,硬膜形成と前顎部,鼻腔側の皮膚形成を行った.術後,合併症は認められず,術後3週間目より放射線療法を,その後,化学療法を行った.術後,1年5ヵ月が経過したが,MRI上では腫瘍の再発を窺わせる所見はない.この症例は症状発症から2ヵ月で生検施行まで至り,さらに頭蓋内浸潤を認めていることから,sinonasal carcinomaの中でもかなり浸潤性,悪性度の高いものと考えられ,放射線照射,化学療法のみでは長期生存が望めないことからも,放射線照射に先立って手術を行った
  • S Kin, M Wanibuschi, Y Minamida, T Yamaki, S Tanabe, K Houkin NEUROLOGICAL SURGERY 31 (6) 677 -682 2003年06月 [査読無し][通常論文]
     
    We report a case of a 66-year-old male with diffuse infiltration of neoplasm from the paranasal sinuses to the orbit and left cerebral hemisphere, associated with prominent edema. Initial complaints were headache and swelling of the forehead, which were followed by progressive symptoms, Such as epistaxis, dacryops, and severe pains. Neuroimaging showed marked invasion of a neoplasm from the left paranasal sinuses into the intracranial space and to the frontal skull.Radical removal of the neoplasm and left orbital content with reconstruction of the anterior skull base using musclocutaneous flap was carried out by a multidisciplinary team. The pathological diagnosis was "poorly differentiated carcinoma from the paranasal sinus". Postoperatively the patient received radiation and chemotherapy. He is still alive with mild right hemiparesis and mild dysphasia, more than 2 years after surgery.
  • 黒田 敏, 中山 若樹, 難波 理奈, 七戸 秀夫, 石川 達哉, 鈴木 明文, 西野 晶子, 宝金 清博, 峰松 一夫, 「若年世代の脳卒中の診断,治療,予防戦略に関する全国多施設共同研究」もやもや病ワーキング・グループ 脳卒中 25 (2) 215 -229 2003年06月 [査読無し][通常論文]
  • 頭蓋咽頭腫におけるp53ファミリーの発現とその意義
    百田 洋之, 一宮 慎吾, 池田 健, 八巻 稔明, 宝金 清博, 佐藤 昇志 Brain Tumor Pathology 20 (Suppl.) 59 -59 2003年05月 [査読無し][通常論文]
  • 【脳腫瘍のキーワード】神経幹細胞移植
    佐々木 祐典, 本望 修, 宝金 清博, 端 和夫 Clinical Neuroscience 21 (5) 542 -542 2003年05月 [査読無し][通常論文]
  • 迷走神経刺激療法が著効したLennox-Gastaut症候群の1女児例
    若井 周治, 二階堂 弘輝, 堤 裕幸, 本望 修, 宝金 清博, 川本 芳誉 日本小児科学会雑誌 107 (5) 849 -849 2003年05月 [査読無し][通常論文]
  • K Kamada, K Houkin, F Takeuchi, N Ishii, J Ikeda, Y Sawamura, S Kuriki, H Kawaguchi, Y Iwasaki SURGICAL NEUROLOGY 59 (5) 353 -362 2003年05月 [査読無し][通常論文]
     
    BACKGROUND In this study, we visualized the eloquent motor system including the somatosensory-motor cortex and corticospinal tract on a neuronavigation system, integrating magnetoencephalography (MEG), functional magnetic resonance imaging (fMRI), and anisotropic diffusion-weighted MRI (ADWI). METHODS Four patients with brain lesions adjacent to the eloquent motor system were studied. Motor-evoked responses (MER) by finger-tapping paradigm were acquired with a 1.5-Tesla MR scanner, and somatosensory-evoked magnetic fields (SEF) by median nerve stimulation were measured with a 204-channel MEG system. In the same fMRI examination, ADWI and anatomic three-dimensional T1-weighted imaging (3-D MRI) were obtained. Activated areas of MER, estimated SEF dipoles, and the corticospinal tract on ADWI were coregistered to 3-D MRI, and the combined MR data were transferred to a neuronavigation system (functional neuronavigation). Intraoperative recording of cortical somatosensory-evoked potentials was performed for confirmation of the central sulcus. RESULTS Combination of fMRI and MEG enabled firm identification of the central sulcus. Functional neuronavigation facilitated extensive tumor resection, having the advantage of sparing the motor cortex and corticospinal tract in all cases. CONCLUSIONS The proposed functional neuronavigation allows neuro-surgeons to perform effective and maximal resection of brain lesions, identifying and sparing eloquent cortical components and their subcortical connections. Potential clinical application of this technique is discussed. (C) 2003 Elsevier Inc. All rights reserved.
  • 宝金 清博, 田中 耕太郎, 松居 徹 臨床と薬物治療 22 (5) 467 -478 2003年05月 [査読無し][通常論文]
  • 佐々木 祐典, 本望 修, 宝金 清博, 端 和夫 最新医学 58 (4) 899 -903 2003年04月 [査読無し][通常論文]
     
    近年の神経科学の進歩により,神経系の発達,再生機構を細胞・遺伝子レベルで制御し,神経再生,神経回路の再構築が可能となり始めている.今後,脳卒中治療においても,失われた中枢神経機能の再建を目的とする神経再生医療が期待されている.そこで,神経幹細胞や骨髄細胞などの幹細胞を利用した細胞療法において,各種の幹細胞の利点・欠点を比較検討し,脳卒中に対する再生医療の展望を概説した
  • 八巻 稔明, 宝金 清博 JIM: Journal of Integrated Medicine 13 (4) 358 -361 2003年04月 [査読無し][通常論文]
  • 本望 修, 鰐淵 昌彦, 南田 善弘, 田邊 純嘉, 宝金 清博 臨床神経生理学 31 (2) 216 -216 2003年04月 [査読無し][通常論文]
  • 脳梗塞治療のガイドラインと新しいパラダイムシフト
    宝金 清博 臨床神経学 43 (4) 203 -203 2003年04月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 12 (4) 301 -301 2003年04月 [査読無し][通常論文]
  • 佐々木 祐典, 本望 修, 宝金 清博, 端 和夫 最新医学 58 (3月増刊) 677 -684 2003年03月 [査読無し][通常論文]
     
    中枢神経疾患に対する細胞移植療法の研究は世界的に注目を集めている.ヒトを含む高等霊長類の中枢神経系は,ニューロン,アストロサイト,オリゴデンドロサイトなどの多様な細胞から構成されているが,近年,これらの細胞の起源である神経幹細胞が発見された.神経幹細胞とは,自己増殖能と多分化能を保持した未分化な細胞である.特に,ヒト成人脳にも神経幹細胞が存在することが判明したことから,同細胞を用いた神経再生医療に期待が集まることになった.神経幹細胞を始めとする様々な細胞をドナーとした細胞移植による神経再生戦略の最新の知見を概説した
  • 脳神経外科手術 脳血管障害 モヤモヤ病に対する血行再建術
    宝金 清博 脳神経外科速報 13 (3) 247 -252 2003年03月 [査読無し][通常論文]
  • 飯星 智史, 本望 修, 備前 明子, 井上 道夫, 本間 敏美, 宝金 清博, 端 和夫 脳卒中 25 (1) 168 -168 2003年03月 [査読無し][通常論文]
  • 脊椎・脊髄外傷の治療 骨傷のない頸髄損傷の病態と外科治療
    小柳 泉, 宝金 清博, 今村 博幸, 岩崎 喜信, 飛騨 一利 日本神経外傷学会プログラム・抄録集 26回 61 -61 2003年03月 [査読無し][通常論文]
  • 神経保護と神経再生 ラット脊髄脱髄疾患モデルに対する自家骨髄移植の投与方法および投与細胞数の比較
    井上 道夫, 本望 修, 岡 真一, 飯星 智史, 宝金 清博, 端 和夫 日本神経外傷学会プログラム・抄録集 26回 129 -129 2003年03月 [査読無し][通常論文]
  • KURODA Satoshi, KOBAYASHI Tohru, ISHII Nobuaki, IKEDA Jun, SHINOHE Yumiko, HOUKIN Kiyohiro, IWASAKI Yoshinobu, NAGASHIMA Kazuo Neuropathology : official journal the Japanese Society of Neuropathology 23 (1) 1 -8 2003年03月01日 [査読無し][通常論文]
     
    Chlamydia pneumoniae (C. pneumoniae) infection has been recently accepted as an important cause of atherosclerosis. However, the precise mechanisms remain unclear. The present study was aimed to clarify the distribution link among C. pneumoniae , chlamydial HSP 60, and activated macrophages. Atheromatous carotid plaques were obtained from 40 consecutive carotid endarterectomies (CEA). The specimens were prepared for HE and elastica-van Gieson staining. Parallel sections were stained immunocytochemically with monoclonal antibodies for a C. pneumoniae -specific antigen, chlamydial HSP 60, activated macrophages, and smooth muscle cells. Immunoreactivity for the C. pneumoniae-specific antigen was observed within the endothelial cells, activated macrophages, and smooth muscle cells in 36 of 40 specimens (90%). Chlamydial HSP 60 was found in all specimens positive for the C. pneumoniae-specific antigen, and mainly co-localized with the C. pneumoniae -specific antigen within the activated macrophages. The present results suggest that C. pneumoniae is a key microbial organ that causes atheroma developments in the carotid artery. Chlamydia pneumoniae -infected macrophages may come into the arterial intima and mediate inflammatory and autoimmune processes through the production of chlamydial HSP 60, leading to atherosclerosis.
  • S Kuroda, T Kobayashi, N Ishii, J Ikeda, Y Shinohe, K Houkin, Y Iwasaki, K Nagashima NEUROPATHOLOGY 23 (1) 1 -8 2003年03月 [査読無し][通常論文]
     
    Chlamydia pneumoniae (C. pneumoniae) infection has been recently accepted as an important cause of atherosclerosis. However, the precise mechanisms remain unclear. The present study was aimed to clarify the distribution link among C. pneumoniae , chlamydial HSP 60, and activated macrophages. Atheromatous carotid plaques were obtained from 40 consecutive carotid endarterectomies (CEA). The specimens were prepared for HE and elastica-van Gieson staining. Parallel sections were stained immunocytochemically with monoclonal antibodies for a C. pneumoniae -specific antigen, chlamydial HSP 60, activated macrophages, and smooth muscle cells. Immunoreactivity for the C. pneumoniae-specific antigen was observed within the endothelial cells, activated macrophages, and smooth muscle cells in 36 of 40 specimens (90%). Chlamydial HSP 60 was found in all specimens positive for the C. pneumoniae-specific antigen, and mainly co-localized with the C. pneumoniae -specific antigen within the activated macrophages. The present results suggest that C. pneumoniae is a key microbial organ that causes atheroma developments in the carotid artery. Chlamydia pneumoniae -infected macrophages may come into the arterial intima and mediate inflammatory and autoimmune processes through the production of chlamydial HSP 60, leading to atherosclerosis.
  • M Hokari, S Kuroda, K Furukawa, K Houkin, Y Iwasaki NEUROLOGICAL SURGERY 31 (3) 281 -286 2003年03月 [査読無し][通常論文]
     
    We report an adult case who presented vertigo attacks due to subclavian steal syndrome associated with the right aortic arch in a patient with Peutz-Jeghers syndrome. A 29-year-old male diagnosed as having Peutz-jeghers syndrome developed frequent vertigo attacks and was admitted to our hospital. Blood pressure of the left arm was 20 mmHg lower than that of the right arm. Aortagraphy showed that he had a right aortic arch and isolation of the left subclavian artery. Right vertebral angiography opacified the left vertebral artery and the subclavian artery in retrograde fashion, suggesting subclavian steal phenomenon. Blood flow studies disclosed impaired reactivity to acetazolamide in the bilateral cerebellar hemispheres. We successfully carried out left common carotid artery-transverse cervical artery bypass. Postoperative angiography confirmed the patency of the graft and the disappearance of subclavian steal phenomenon. Vasoreactivity to acetazolamide normalized in the cerebellum. Vertigo attacks were not noted during the one-year follow-up period.
  • 【脊髄再生・移植に関する基礎知識】神経再生医療の基礎知識
    佐々木 祐典, 本望 修, 宝金 清博 脊椎脊髄ジャーナル 16 (2) 101 -105 2003年02月 [査読無し][通常論文]
  • 骨傷のない頸髄損傷の外科治療
    小柳 泉, 宝金 清博, 今村 博幸, 三森 研自, 岩崎 喜信 日本脳神経外科救急学会プログラム・抄録集 8回 75 -75 2003年01月 [査読無し][通常論文]
  • 中山若樹, 宝金清博, 黒田敏 モヤモヤ病(ウィリス動脈輪閉塞症)に関する調査研究 平成14年度総括・分担研究報告書 9-13 -13 2003年 [査読無し][通常論文]
  • T Kobayashi, S Kuroda, M Tada, K Houkin, Y Iwasaki, H Abe BRAIN RESEARCH 960 (1-2) 62 -70 2003年01月 [査読無し][通常論文]
     
    The objective of the present study, was to determine the biochemical characteristic of Ca2+-induced mitochondrial swelling (mitochondrial permeability transition: mPT) and cytochrome c release in the brain. and to clarify its role in neuronal injury following transient forebrain ischemia. Mitochondria were isolated from rat brain and liver. Changes in mitochondrial volume were measured via light absorbance at 540 nm. Using Western blot analysis, we examined the in vitro release of mitoehondrial cytochrome c under these conditions. Transient forebrain ischemia was induced by 5 min occlusion of the common carotid arteries in the gerbil, Cyclosparin A (CsA), a specific mPT blocker, and/or trifluoperazine. a blocker of phospholipase were given before and 24 h after ischemia. The number of surviving neurons in the hippocampal CA1 sector was counted 7 days after ischemia. Calcium induced a moderate decrease of light absorbance in brain mitochondria. which was inhibited by CsA. However, Calcium induced a much larger decrease of light absorbance in liver mitochondria. Calcium induced a moderate release of cytochrome c from brain mitochondria. which was not inhibited by CsA. However. calcium induced the release of a larger amount of cytochrome c from liver mitochondria. Selective neuronal injury due to transient forebrain ischemia was significantly ameliorated by treatment with high-dose CsA. The biochemical properties of Ca2+-induced mitochondrial swelling in the brain are different from those in the liver. Cytochrome c is released from brain mitochondria through in mPT-independent mechanism. CsA potentially ameliorates delayed neuronal injury in file hippocampus due to transient forebrain ischemia. (C) 2002 Elsevier Science B.V. All rights reserved.
  • Hiroyuki Momota, Shingo Ichimiya, Tatsuru Ikeda, Toshiaki Yamaki, Tomoki Kikuchi, Kiyohiro Houkin, Noriyuki Sato Brain Tumor Pathology 20 (2) 73 -77 2003年 [査読無し][通常論文]
     
    Craniopharyngiomas are intracranial tumors that usually arise in the site around the sella turcica. They are composed of distinctive sheets of epithelial cells showing adamantinomatous or squamous-papillary histologic types. Because little is known about the tumorigenesis of craniopharyngiomas, we retrieved samples from 15 tumor cases to investigate the functional significance of the p53 family of transcription factors, which are known to be expressed in various human epithelia. Immunohistochemical analysis of these cases demonstrated similar expression profiles of p53 family members in the two histologic types of the tumor i.e., strong nuclear expression of p63 was observed in all cell layers, and moderate to intense nuclear expression of p73 was observed in the basal cell layers. In contrast to p63 and p73, the reactivity of an archetypal tumor suppressor, p53, was occasional and weak in the two histologic types. Because p63 was widely expressed in the tumors, reverse transcription-polymerase chain reaction (RT-PCR) analysis was conducted to elucidate which spliced variant of p63 was expressed. The results showed that ANp63, lacking a terminal transactivation domain of p63, was the dominant isoform. Together with the reported evidence that the ANp63 isoform is highly expressed in human squamous-cell carcinomas, these data suggest that the cellular architecture characteristic of the expression of p53 family members may be required for the histogenesis of craniopharyngiomas, where ANp63 has a possible role in maintaining proliferative activity of the tumor cells, like squamous-cell carcinomas in other tissues.
  • Moyamoya Disease
    Batjer and Loftus: Textbook of Neurological Surgery 2003年 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 12 (1) 16 -24 2003年01月 [査読無し][通常論文]
     
    内頸動脈血栓内膜剥離術(CEA)は,evidenceに基づいた外科治療の代表的なものである.外科治療にあたっては,適応,外科解剖,手術手順,危険回避のためのさまざまな知識と工夫が必要である.頸動脈へのアプローチでは,的確な表面解剖の知識,頸動脈に至るまでの神経,血管の走行,機能に関して十分な知識が必要である.顕微鏡下に内膜剥離を行うが,内シャントチューブは,実際に入れるか入れないかは別として,その知識と技術を修得し,その扱いが完全にできるようにしておくことは,本手術を安全に行うためには必須である.CEAは,的確に行うことにより,高い安全性が保障されているが,逆に,少しでも適応,術前検査,手術手技,危険回避の知識に関して不十分な点があると,大きな合併症を引き起こすものである.危険回避の方法あるいは不測の事態に対する対処に関しても,十分な対策をsimulateしておくことが重要である.
  • 藤井 清孝, 宝金 清博 脳卒中 24 (4) 474 -474 2002年12月25日 [査読無し][通常論文]
  • 虚血から脳を守る 脳虚血治療のfrontline
    宝金 清博 脳神経外科速報 12 (12) 931 -934 2002年12月 [査読無し][通常論文]
  • 黒田 敏, 石川 達哉, 宝金 清博, 岩崎 喜信 Neurological Surgery 30 (12) 1295 -1300 2002年12月 [査読無し][通常論文]
     
    標題の臨床的意義を検討するため,もやもや病患者136例について後大脳動脈狭窄性病変(以下狭窄性病変)の頻度や特徴を調査し次のような結果を得た.40例(30.3%)に狭窄性病変を認めた.虚血型もやもや病では出血型に比べて高率に狭窄性病変を合併していた.狭窄性病変を有する大脳半球では頭頂葉を中心とする領域に梗塞や循環障害を高率に認めた.狭窄性病変のある群では内頸動脈領域に対する血行再建術の後も10%で後頭葉の虚血症状が出現していた.MR angiographyを用いて長期に観察した結果,小児の狭窄性病変は安定したものが多かった
  • S Kuroda, T Ishikawa, K Houkin, Y Iwasaki NEUROLOGICAL SURGERY 30 (12) 1295 -1300 2002年12月 [査読無し][通常論文]
     
    Purpose : The present study was aimed at clarifying the clinical significance of posterior cerebral artery (PCA) stenosis/occlusion in pediatric and adult moyamoya disease. Subjects and Methods : This study included a total of 132 patients (52 children and 80 adults) who were diagnosed as by cerebral angiography having moyamoya disease. CT or MRI was performed to examine the location of cerebral infarction in all subjects. Cerebral blood flow and vasoreactivity to acetazolamide were measured in 80 patients before surgery, using single photon emission computed tomography (SPECT). Three-dimensional MR angiography (3D-MRA) was repeated in 32 pediatric patients after surgery in order to clarify the natural course of the PCA stenosis/occlusion. Results : Of 264 sides in 132 patients, PCA stenosis/occlusion was observed in 50 sides of 40 patients (30.3%). Its incidence was significantly higher in ischemic-type patients than in hemorrhagic-type and asymptomatic patients, and was higher in patients in the advanced stage of the disease. The hemisphere ipsilateral to PCA stenosis/occlusion had higher incidence of ischemic symptoms, cerebral infarction, and impaired cerebral hemodynamics. TIA (hemianopsia) or cerebral infarction in the occipital lobe was noted in 4 (10%) of 40 patients during follow-up periods after bypass surgery for anterior circulation. Of 32 pediatric patients, none showed progression of PCA stenosis on 3D-MRA during follow-up periods. Conclusions : The present study showed that the involvement of PCA could increase the risk of TIA and/or cerebral infarction in both anterior and posterior circulation areas, suggesting that the PCA plays an important collateral role in moyamoya disease.
  • 術後耐性菌感染症への対応 脳外科手術における毛髪処理法と術後感染症
    南田 善弘, 端 和夫, 宝金 清博 日本化学療法学会雑誌 50 (Suppl.B) 33 -33 2002年11月 [査読無し][通常論文]
  • 難波 理奈, 黒田 敏, 岩崎 喜信, 多田 光宏, 寳金 清博 新潟医学会雑誌 116 (10) 508 -509 2002年10月10日 [査読無し][通常論文]
  • 中山若樹, 上山博康, 磯部正則, 滝沢克己, 数又研, 石川達哉, 黒田敏, 岩崎喜信, 宝金清博 日本脳神経外科学会総会抄録集 61st (CD-ROM Abstracts) 28.1A102 2002年10月02日 [査読無し][通常論文]
  • ヒトグリオーマにおけるHLA-class II発現機構の解析と癌免疫
    高村 幸夫, 池田 英之, 金関 貴幸, 宝金 清博, 佐藤 昇志 日本癌学会総会記事 61回 149 -149 2002年10月 [査読無し][通常論文]
  • 北村 淳, 黒田 敏, 牛越 聡, 古川 浩司, 浅野 剛, 七戸 秀夫, 宝金 清博, 岩崎 喜信, 斉藤 久壽, 三森 研自 Neurological Surgery 30 (10) 1097 -1102 2002年10月 [査読無し][通常論文]
     
    舌癌或いは喉頭癌に対して放射線治療の既往があり,頸部内頸動脈狭窄症を認めた3例を対象とし,ステントを併用した血管形成術を施行した.ステントを併用した経皮的血管形成術(PTA)により,全ての病変において十分な狭窄の改善が得られた.狭窄率は,いずれも28%以下と著明に改善した.周術期に脳虚血症状は出現しなかった.経過観察期間中に,DSA又は頸動脈エコーにおいて明らかな再狭窄は認められず,新たな脳梗塞の再発も観察されなかった.合併症は1例で見られ,PTAを行った直後に痙攣が出現したが,その後,消失した
  • 宝金 清博, 難波 理奈, 黒田 敏, 多田 光宏 脳と循環 7 (4) 301 -305 2002年10月 [査読無し][通常論文]
  • 研修医のためのクリニカルカンファレンス 末梢性中大脳動脈解離性動脈瘤
    米増 保之, 南田 善弘, 宝金 清博 脳神経外科速報 12 (10) 775 -779 2002年10月 [査読無し][通常論文]
  • 当科で放射線治療を行った小児脳腫瘍患者の予後についての検討
    染谷 正規, 中田 健生, 永倉 久泰, 大内 敦, 坂田 耕一, 晴山 雅人, 宝金 清博, 鈴木 信寛, 工藤 亨, 中村 博彦, 高橋 義男 小児がん 39 (2) 261 -262 2002年10月 [査読無し][通常論文]
  • ヒトグリオーマにおけるHLA-class II発現機構の解析と癌免疫
    高村 幸夫, 池田 英之, 金関 貴幸, 宝金 清博, 佐藤 昇志 日本免疫学会総会・学術集会記録 32 216 -216 2002年10月 [査読無し][通常論文]
  • S Terasaka, K Itamoto, K Houkin NEUROSURGERY 51 (4) 1083 -1087 2002年10月 [査読有り][通常論文]
     
    OBJECTIVE: We discuss a detailed surgical technique, its indications, and potential pitfalls in its use. METHODS: A:surgical procedure combining Kawase's anterior petrosectomy and external carotid artery-to-posterior cerebral artery high-flow bypass was performed for the treatment of a patient with a fusiform basilar trunk aneurysm. RESULTS: Follow-up angiography revealed the successful obliteration of the aneurysm and that the graft was functioning well. Late ischemic complications, however, occurred 5 weeks after surgery. CONCLUSION: Kawase's approach is a versatile option for safe Vascular reconstruction of the posterior cerebral artery and exposure of a basilar trunk aneurysm in the upper clival region. Although, theoretically, a high-flow bypass might decrease the risk of hemodynamic ischemia, it does not prevent thromboembolic complications.
  • J Kitamura, S Kuroda, S Ushikoshi, K Furukawa, T Asano, H Shichinohe, K Houkin, Y Iwasaki, H Saitoh, K Mitsumori NEUROLOGICAL SURGERY 30 (10) 1097 -1102 2002年10月 [査読無し][通常論文]
     
    We report three cases of radiation-induced carotid arterial stenosis that underwent successful angioplasty with stenting. The patients had received radiation therapy for tongue or laryngeal cancers and developed minor completed strokes 6 to 14 years after irradiation. All patients had multiple and bilateral stenosis, measuring more than 50%, of the carotid arteries. The stenosis was located in the internal, external, and common carotid arteries. We performed percutaneous transluminal angioplasty with stenting. All interventions were successful and carotid stenosis decreased to less than 28%. No permanent complications occurred. During follow-up periods of Lip to 26 months, all of these cases were free from ischemic symptoms. Neither carotid angiography nor ultrasound sonography showed evidence of restenosis. The present results suggest the usefulness of angioplasty with stenting for radiation-induced carotid arterial steriosis.
  • 北村 淳, 黒田 敏, 岩崎 喜信, 北村 淳, 牛越 聡, 寳金 清博 新潟医学会雑誌 = 新潟医学会雑誌 116 (10) 508 -508 2002年10月 [査読無し][通常論文]
  • 黒田 敏, 池田 潤, 中山 若樹, 石川 達哉, 岩崎 喜信, 寳金 清博, 上山 博康 新潟医学会雑誌 = 新潟医学会雑誌 116 (10) 509 -509 2002年10月 [査読無し][通常論文]
  • 中山 若樹, 黒田 敏, 宝金 清博, 石川 達哉, 安田 宏, 古川 浩司, 新谷 好正, 小林 徹, 岩崎 喜信 新潟医学会雑誌 116 (10) 510 -511 2002年10月 [査読無し][通常論文]
  • 脳磁図と機能磁気共鳴画像による前頭,側頭葉言語優位性の機能評価
    鎌田 恭輔, 宝金 清博, 岩崎 喜信, 三森 研自 脳循環代謝 14 (3) 194 -195 2002年09月 [査読無し][通常論文]
  • 頸動脈閉塞性疾患におけるacetazolamide反応性とPETパラメータとの関連について 定量的解析
    黒田 敏, 宝金 清博, 岩崎 喜信, 志賀 哲, 加藤 千恵次, 玉木 長良 脳循環代謝 14 (3) 213 -214 2002年09月 [査読無し][通常論文]
  • K Kamada, K Houkin, Y Iwasaki, F Takeuchi, S Kuriki, K Mitsumori, Y Sawamura JOURNAL OF NEUROSURGERY 97 (3) 558 -567 2002年09月 [査読無し][通常論文]
     
    Object. To identify the primary motor area (PMA) quickly and correctly, the authors used magnetic resonance (MR) axonography, including anisotropic diffusion-weighted (DW) MR imaging and three-dimensional anisotropic contrast (3DAC) imaging, which was performed to visualize the corticospinal tract mainly originating from the PMA. Methods. All studies were obtained in 10 normal volunteers and in 17 patients with brain tumors affecting the central motor system. Data sets of anisotropic DW imaging and anatomical and functional (f)MR imaging were acquired while the participants executed simple hand movements. Offline processing of 3DAC MR axonography images was subsequently done to extract only the anisotropic components of the tract fibers. Somatosensory evoked fields (SSEFs) and intraoperative cortical somatosensory evoked potentials (SSEPs) were recorded after electrical stimulation of the median nerve. Conclusions. In normal volunteers, anisotropic DW imaging, 3DAC imaging, fMR imaging, and magnetoencephalography consistently localized the PMA in both hemispheres. In contrast, fMR imaging and SSEFs failed to identify the PMA in seven and one of the 17 patients, respectively, because of cortical dysfunctions due to brain tumor. The anisotropic DW imaging data acquired within 30 seconds with no patient tasks successfully identified the PMA in 12 patients, and failed in five patients because of the lesions involving the frontal lobe. The anisotropic axonal components were distinctly visualized on 3DAC images and indicated the PMA location, which was confirmed on intraoperative SSEPs in all 17 affected hemispheres. Swift and noninvasive PMA identification by rapid scanning with MR axonography is a promising method for routine clinical use and is especially beneficial for patients who have severe cortical dysfunction in the PMA.
  • 本望 修, 岡 真一, 佐々木 祐典, 高橋 明, 田之岡 篤, 宝金 清博, 端 和夫 脳卒中 24 (3) 355 -358 2002年09月 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博, 石川 達哉, 中山 若樹, 難波 理奈, 上山 博康, 中川 翼, 岩崎 喜信, 阿部 弘 脳卒中の外科 30 (5) 369 -374 2002年09月 [査読無し][通常論文]
     
    過去20年間に経験したもやもや病149例を対象に,脳血行再建術後の長期予後について検討した.その結果,小児例61例全例,成人例88例中74例(虚血型40/40,出血型34/42,無症型0/6)に脳血行再建術が行われた.小児例では脳血行再建術の術式に拘わらず,長期予後は95.1%で良好であることが確認された.術後1年以内に3例(4.9%)でTIAの残存がみられたが,その頻度は術前に比べ有意に減少した.しかし脳梗塞で発症した例では有意なtotal IQの低下を認めた.成人例では虚血型,出血型に関わらず,間接的血行再建術(EMSまたはEMAS)で側副血行路の形成を認めた症例は60%と低く,STA-MCA anatomosisを行った症例ではほぼ全例で長期の開存がみられた.虚血型の2例(5.0%),出血型の8例(20.6%)でそれぞれ術後1〜2年後,術後平均8.1年後に頭蓋内出血を認めた.無症候型は経過観察としたが平均1.8年間の観察期間中に脳虚血発作や頭蓋内出血はみられない
  • S Kuroda, K Houkin, J Ikeda, N Nakayama, T Ishikawa, H Kamiyama, Y Iwasaki, H Abe NEUROSURGERY 51 (2) 563 -563 2002年08月 [査読無し][通常論文]
  • N Nakayama, K Houkin, S Kuroda, H Kamiyama, H Abe, Y Iwasaki NEUROSURGERY 51 (2) 573 -573 2002年08月 [査読無し][通常論文]
  • 宝金 清博 分子脳血管病 1 (3) 277 -283 2002年07月 [査読無し][通常論文]
     
    アポトーシスの画像化は,現在,in vitro,in vivoレベルの研究がはじまったばかりといってよい状況である.アポトーシスの初期にみられる細胞膜におけるホスファチジルセリンの発現を利用して核医学的なmarkerを用いる方法と,MRI用造影剤を用いる方法が知られている.臨床レベルで,apoptosis specificな画像法は,いまのところない.しかし,従来の画像modalityを用いても,脳梗塞,低酸素虚血脳損傷,アルツハイマー病などにおける画像から,その特徴を抽出することは可能である.アポトーシスが,どれほど,こうした病態に関与しているかを明らかにするためにも,そして,アポトーシスにも適応される可能性のあるtherapeutic time window内での治療を開発するためにも,臨床レベルにおけるアポトーシスの画像化は,今後,重要な研究対象となる(著者抄録)
  • 専門医に求められる最新の知識 MRI拡散強調画像(DWI)と灌流画像(PI)
    古明地 孝宏, 鈴木 進, 金 相年, 斉藤 孝次, 宝金 清博 脳神経外科速報 12 (6) 411 -423 2002年06月 [査読無し][通常論文]
  • 骨髄細胞を用いた神経再生医療
    本望 修, 岡 真一, 井上 道夫, 飯星 智史, 宝金 清博, 端 和夫 神経組織の成長・再生・移植研究会学術集会プログラム・予稿集 17回 34 -34 2002年06月 [査読無し][通常論文]
  • 経静脈的自家骨髄細胞移植による脳梗塞の治療
    飯星 智史, 本望 修, 岡 真一, 井上 道夫, 宝金 清博, 端 和夫 神経組織の成長・再生・移植研究会学術集会プログラム・予稿集 17回 36 -36 2002年06月 [査読無し][通常論文]
  • 中枢性脱髄疾患モデルに対する経静脈的自家骨髄移植
    井上 道夫, 本望 修, 岡 真一, 飯星 智史, 宝金 清博, 端 和夫 神経組織の成長・再生・移植研究会学術集会プログラム・予稿集 17回 38 -38 2002年06月 [査読無し][通常論文]
  • 頸部エコー検査によるステント留置後の経過観察
    井上 道夫, 吉藤 和久, 松野 太, 野中 雅, 宝金 清博 脳神経外科速報 12 (5) 343 -349 2002年05月 [査読無し][通常論文]
     
    ステント留置部におけるカラードプラとパルスドプラの総合所見を血管撮影と比較,頸部エコーがステント後のfollow-upとしてどの程度信用できるかを検討した.2000年9月から2001年2月迄にステント留置をした5症例,6病変を対象とした.ステント自体によるartifactは,殆どみられなかった.ステント内腔は,頸部エコーで十分に観察可能であった.カラードプラとパルスドプラを組み合わせて検査を行うと信頼性が向上した.頸部エコー検査のみでは再狭窄の確定診断は困難なことがあった.再狭窄のスクリーニングとして,血管撮影の適応を判断するのに有用であった
  • 専門医に求められる最新の知識 神経幹細胞移植
    井上 道夫, 本望 修, 宝金 清博 脳神経外科速報 12 (5) 331 -336 2002年05月 [査読無し][通常論文]
  • 鎌田 恭輔, 宝金 清博, 岩崎 喜信, 三森 研自 臨床神経生理学 30 (2) 186 -186 2002年04月 [査読無し][通常論文]
  • 難波 理奈, 黒田 敏, 岩崎 喜信, 多田 光宏, 宝金 清博 脳卒中 24 (1) 76 -76 2002年04月 [査読無し][通常論文]
  • 本望 修, 岡 真一, 佐々木 祐典, 高橋 明, 田之岡 篤, 宝金 清博, 端 和夫 脳卒中 24 (1) 141 -141 2002年04月 [査読無し][通常論文]
  • S Ushikoshi, K Houkin, S Kuroda, T Asano, Y Iwasaki, K Miyasaka, H Abe SURGICAL NEUROLOGY 57 (4) 253 -261 2002年04月 [査読無し][通常論文]
     
    BACKGROUND When considering the treatment strategies for dural arteriovenous fistulas (DAVFs), it is important to clarify the exact location of the fistula and venous drainage route from both DAVFs and normal brain tissue. DAVFs with leptomeningeal retrograde venous drainage carry a high risk of neurological deficits and require aggressive treatment. When AVFs involve the dural sinus, transvenous embolization via the transfemoral approach is usually the first choice of treatment. For DAVFs draining directly into the cortical veins without dural sinus involvement, transarterial embolization may be a curative treatment. However, when embolization is technically difficult or results in incomplete occlusion, surgical treatment is required. The purpose of the present study was to review our experience with surgical treatment of DAVFs. METHODS The present series included 17 patients; 9 had DAVFs involving the dural sinus with leptomeningeal retrograde venous drainage and 8 had DAVFs draining directly into the cortical veins. For DAVFs involving the sinus, embolization of the diseased sinus by direct surgical exposure was performed in 8 patients, and surgical excision in one. For DAVFs draining directly into the cortical veins, interruption of the draining veins close to DAVFs was undertaken in 7 and surgical excision in 1. RESULTS Complete obliteration of DAVFs was demonstrated in 16 patients. At final follow-up, 15 patients were asymptomatic and the other 2 showed clinical improvement. CONCLUSIONS For DAVFs involving the dural sinus, direct operative sinus packing is indicated. For DAVFs directly draining into the cortical veins, surgical interruption of the draining veins is indicated. (C) 2002 by Elsevier Science Inc.
  • もやもや病の脳循環代謝 SPECT/PETによる検討
    七戸 秀夫, 黒田 敏, 宝金 清博, 岩崎 喜信, 志賀 哲, 加藤 千恵次, 玉木 長良 脳循環代謝 14 (1) 81 -81 2002年03月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 11 (2) 140 -140 2002年02月 [査読無し][通常論文]
  • H Shichinohe, S Kuroda, K Houkin, Y Iwasaki STROKE 33 (1) 407 -407 2002年01月 [査読無し][通常論文]
  • N Nakayama, K Houkin, S Kuroda, Y Iwasaki STROKE 33 (1) 368 -368 2002年01月 [査読無し][通常論文]
  • S Kuroda, K Houkin, H Kamiyama, Y Iwasaki, H Abe STROKE 33 (1) 347 -347 2002年01月 [査読無し][通常論文]
  • K Kamada, K Mitsumori, K Houkin, Y Iwasaki STROKE 33 (1) 357 -357 2002年01月 [査読無し][通常論文]
  • 中山若樹, 宝金清博, 黒田敏 ウィリス動脈輪閉塞症の病因・病態に関する研究 平成13年度総括・分担研究報告書 39-43 -43 2002年 [査読無し][通常論文]
  • 中山若樹, 宝金清博, 黒田敏 ウィリス動脈輪閉塞症の病因・病態に関する研究 平成11−13年度総合研究報告書 25-29 -29 2002年 [査読無し][通常論文]
  • 小林 徹, 宝金 清博, 黒田 敏, 斉藤 久寿, 岩崎 喜信 脳卒中の外科 30 (1) 15 -18 2002年01月 [査読無し][通常論文]
     
    1992〜1999年に視力障害で発症した内頸動脈閉塞症10例(49〜69歳,全例男)を対象とし,発症時の症状,脳神経外科受診に至る迄の経緯,眼症状,血管造影所見,SPECT所見を検討した.症状発現時,眼科を受診したものは7例,直接脳神経外科を受診したのが3例であった.視力障害は,来院時の視力に0.5以上の左右差が存在したものは6例であった.虚血性網膜症を全例に認めたが,既に二次性の新生血管緑内障を合併しているものが7例認められた.7例に,糖尿病の合併がみられた.血管造影を施行,全例で内頸動脈は起始部で閉塞していた.STA-MCA anastomosisはSPECTでType 3を示した5例に対し施行した.術後全例で眼動脈の逆行性血流が消失し,SPECTでは脳血流の改善が認められた.術後視力が著明に改善した例はなかったものの2例では網膜循環時間の改善が認められた.又,虹彩の新生血管は全例で減少した
  • 牛越 聡, 宝金 清博, 黒田 敏, 宮坂 和男, 岩崎 喜信 Neurological Surgery 29 (12) 1215 -1220 2001年12月 [査読無し][通常論文]
     
    標記塞栓術を行い良好な結果が得られた3例を報告した.3例ともcomplete clippingを目指すと血管を損傷する可能性が高いと術中に判断し,頸部を形成するような不完全clippingとし術後に塞栓術を行った.いずれの症例も,頭部の狭い動脈瘤へ変化していたこと,又,clipがよいメルクマークとなり,母血管と動脈瘤がよく分離される透視角度の設定がしやすかったことから比較的容易に完全閉塞を達成し得た.clipによる動脈瘤の変形など,clipの存在が塞栓術の妨げとなるような症例はなかった
  • 脳主幹動脈閉塞性疾患における脳酸素代謝とacetazolamide反応性
    黒田 敏, 宝金 清博, 志賀 哲, 加藤 千恵次, 玉木 長良, 岩崎 喜信, 阿部 弘 脳循環代謝 13 (4) 328 -328 2001年12月 [査読無し][通常論文]
  • S Ushikoshi, K Houkin, S Kuroda, K Miyasaka, Y Iwasaki NEUROLOGICAL SURGERY 29 (12) 1215 -1220 2001年12月 [査読無し][通常論文]
     
    Objective: We report three patients with broad-necked distal basilar artery (BA) aneurysms treated with intentional incomplete clipping followed by endovascular occlusion using Guglielmi detachable coils. Methods: The location of the aneurysms was BA bifurcation in one patient and BA-superior cerebellar artery (SCA) in two. One patient presented with acute subarachnoid hemorrhage and two patients had incidental aneurysms. In two patients, endovascular treatment was thought to be difficult considering the morphology of the aneurysms and surgical treatment was performed as the first choice of treatment. One patient with a BA-SCA aneurysm underwent endovascular treatment using a remodelling technique first. However, it was impossible to place the coil preserving SCA, so surgical treatment was performed. In all patients, the attempt to pursue complete clipping was considered to be accompanied with high risks of morbidity, so neck-plastic incomplete clipping was performed intentionally. One to six days after the surgery, coil embolization was performed. Results: In all patients, complete occlusion of the aneurysms was achieved and all patients had excellent clinical outcomes. Conclusion: Intentional neck-plastic incomplete clipping followed by endovascular coiling may be a useful treatment option for patients with broad-necked distal BA aneurysms.
  • 矢野 俊介, 宝金 清博, 阿部 悟, 齋藤 久寿, 黒田 敏, 浅野 剛, 牛越 聡, 岩崎 喜信 Neurological Surgery 29 (11) 1081 -1090 2001年11月 [査読無し][通常論文]
     
    69歳,49歳,50歳男.いずれも内科的治療に抵抗性を示す患者であった.3例とも手術中とくに問題は生じず,術後経過良好であった
  • 黒田 敏, 宝金 清博, 岩崎 喜信, 阿部 弘, 志賀 哲, 加藤 千恵次, 玉木 長良 新潟医学会雑誌 115 (11) 599 -599 2001年11月 [査読無し][通常論文]
  • 鎌田 恭輔, 三森 研自, 北見 公一, 小柳 泉, 能條 建, 橋本 学, 宝金 清博, 岩崎 喜信 新潟医学会雑誌 115 (11) 600 -600 2001年11月 [査読無し][通常論文]
  • 中山若樹, 宝金清博, 岩崎喜信, 斎藤久寿 日本脳循環代謝学会総会プログラム・抄録集 13th 108 2001年10月18日 [査読無し][通常論文]
  • 中山若樹, 宝金清博, 黒田敏, 岩崎喜信 日本脳神経外科学会総会抄録集 60th (Abstract) 205 2001年10月15日 [査読無し][通常論文]
  • 宝金清博, 黒田敏, 岩崎喜信, 滝川修吾, 中山若樹, 斉藤久寿 日本脳神経外科学会総会抄録集 60th (Abstract) 139 2001年10月15日 [査読無し][通常論文]
  • 瀧川修吾, 沢村豊, 宝金清博, 中山若樹, 斎藤久寿 日本脳神経外科学会総会抄録集 60th (Abstract) 292 2001年10月15日 [査読無し][通常論文]
  • Acetazolamide testは内頸動脈・中大脳動脈閉塞症の長期予後を予測できるか?
    黒田 敏, 宝金 清博, 上山 博康, 池田 潤, 斉藤 久壽, 三森 研自, 能條 建, 柏葉 武, 岩崎 喜信, 阿部 弘 脳循環代謝 13 (3) 142 -142 2001年10月 [査読無し][通常論文]
  • 脳虚血におけるmitochondrial permeability transition(MPT)の役割に関する研究
    小林 徹, 黒田 敏, 宝金 清博, 多田 光宏, 岩崎 喜信, 阿部 弘 脳循環代謝 13 (3) 187 -188 2001年10月 [査読無し][通常論文]
  • 七戸 秀夫, 黒田 敏, 宝金 清博, 牛越 聡, 岩崎 喜信, 阿部 弘 Neurological Surgery 29 (9) 871 -876 2001年09月 [査読無し][通常論文]
     
    視野障害を合併する内頸動脈高度狭窄症に対して,内頸動脈stent留置術を施行し,術前に低下していた後大脳動脈領域の脳血流量が増加し,症状が改善した症例を2例経験した.2例に共通する病態として,脳血管撮影では内頸動脈に高度狭窄が存在し,同側の後交通動脈が太く,これによって内頸動脈領域への側副血行路がよく発達していた.この為,SPECTでは反対側の後大脳動脈の脳血流量やacetazolamide反応性が低下し,その後頭葉に起因する視野狭窄を認めた.症例1(64歳男)では後頭葉に小さな脳梗塞もみられた.頸動脈狭窄症に対してstentを併用した経皮的血管形成術を実施したところ,後頭葉の脳循環が改善すると共に視野障害が軽快した.特に,症状の改善は脳梗塞を有さなかった症例2(64歳男)において顕著であった.いずれの症例においても椎骨動脈の起始部やその遠位部に閉塞性病変は認められなかった
  • S Kuroda, K Houkin, H Kamiyama, K Mitsumori, Y Iwasaki, H Abe STROKE 32 (9) 2110 -2115 2001年09月 [査読無し][通常論文]
     
    Background and Purpose-The importance of hemodynamic parameters for predicting outcome in patients with occlusive carotid disease remains controversial. The present study was aimed at testing the hypothesis that regional cerebrovascular reactivity (rCVR) to acetazolamide can be a reliable predictor of subsequent ischemic stroke in medically treated patients with internal carotid artery or middle cerebral artery occlusion. Methods-Seventy-seven symptomatic patients were enrolled in this prospective, longitudinal cohort study. All patients met inclusion criteria of cerebral angiography, no or localized cerebral infarction on MRI or CT, and no or minimal neurological deficit. Regional cerebral blood flow (rCBF) and rCVR to acetazolamide were quantitatively determined by Xe-133 SEPCT. All patients were categorized into 4 types on the basis of SPECT studies. Results-During an average follow-up period of 42.7 months, 16 total and 7 ipsilateral ischemic strokes occurred. The annual risks of total and ipsilateral stroke in patients with decreased rCBF and rCVR were 35.6% and 23.7%, respectively, risks that are higher than those in other types of patients. When strokes were categorized into patients with and without decreased rCBF and rCVR, Kaplan-Meier analysis revealed that the risks of total and ipsilateral stroke in patients with decreased rCBF and rCVR were significantly higher than in those without (P < 0.0001 and P = 0.0001, respectively, log-rank test). Relative risk conferred by decreased rCBF and rCVR was 8.0 (95% Cl, 1.9 to 34.4) for ipsilateral stroke and 3.6 (95% CI, 1.4 to 9.3) for total stroke. Conclusions-Decreased rCBF and rCVR to acetazolamide may identify a subgroup of patients who have a higher risk of subsequent ischemic stroke when treated medically.
  • H Shichinohe, S Kuroda, K Houkin, S Ushikoshi, Y Iwasaki, H Abe NEUROLOGICAL SURGERY 29 (9) 871 -876 2001年09月 [査読無し][通常論文]
     
    Although previous reports have suggested "steal VBI" due to occlusive carotid artery diseases, there have been no reports that clearly define "steal VBI" from the viewpoint of cerebral hemodynamics. The authors presented two cases with "steal VBI" due to severe stenosis of the internal carotid artery. Both patients had well-developed collateral circulation through the ipsilateral posterior communicating artery. Although no occlusive lesion was found in the vertebrobasilar system, blood flow studies revealed impaired hemodynamics in the contralateral occipital lobe, which fact correlated with their neurological deficit, visual field disturbance. Carotid stenting markedly corrected the stenotic lesions, leading to neurological improvement. Follow-up blood studies showed normalization of hemodynamics in the contralateral occipital lobe. The findings strongly suggest that carotid surgery or stenting can improve cerebral hemodynamics in the carotid systems, resolving "steal VBI" due to developed collaterals from the posterior to the anterior circulation.
  • 小林 徹, 黒田 敏, 宝金 清博, 岩崎 喜信, 阿部 弘 脳卒中の外科 29 (5) 345 -350 2001年09月 [査読無し][通常論文]
     
    近赤外線スペクトロスコピー(NIRS)により観察した頸動脈遮断時NIRSパラメータ変化の有用性と問題点について検討した.NIRSを用いてモニタリングを行ったcarotid endarterectomy(CEA)55例,carotid balloon occlusion test(BOT)16例,vertebral artery(VA)transpositionの際の総頸動脈遮断時に測定した4例の計75症例を対象とした.NIRSの測定と同時に体性感覚誘発電位(SEP)を,一部症例ではtranscranial Doppler sonography(TCD)とSPECTでのモニタリングを併用した.その結果,NIRSはCEAや頸動脈BOTなど頸動脈閉塞を必要とする手技において,脳循環代謝の変化をモニタリングするのに極めて有用であり,TCD・SPECT・SEPの測定結果ともよく相関した.総頸動脈閉塞例ではNIRSのみでは正確な評価はできないこと,プローベの設置位置の制約から内頸動脈灌流領域全体の変化を反映しないという問題点はあるが,SEP等のモニタリングとの組み合わせにより補完可能と考えられた
  • 脳虚血の診断 SPECT,PET Acetazolamide Testは内頸動脈・中大脳動脈閉塞症の長期予後を予測できるか?
    黒田 敏, 宝金 清博, 上山 博康, 池田 潤, 三森 研自, 能條 建, 斎藤 久壽, 柏葉 武, 布村 充, 岩崎 喜信, 阿部 弘 The Mt. Fuji Workshop on CVD 19 14 -17 2001年07月 [査読無し][通常論文]
     
    内科的治療のみを行った内頸動脈(ICA),或いは,中大脳動脈(MCA)の閉塞症例に対象を限定して,acetazolamide反応性を定量的に測定して,長期予後とacetazolamide反応性との関係について検討した.SPECTの結果から,type 1,type 2,type 3,type 4に病型に分類できた.経過観察期間中に,全ての脳梗塞(TS)が16例に,閉塞血管と同側の脳梗塞(IS)が7例に認められた.各病型におけるTSの年間発症率は3.8%,5.2%,35.6%,2.4%であり,ISは0.6%,1.7%,23.7%,2.4%であった.Type 3の年間発生率がほかのtypeの症例よりも明らかに高値であった.SPECT上のtype 3虚血は脳梗塞の再発に関して有意な危険因子であることが判明した
  • 脳虚血 病態診断の進歩と限界 脳主幹動脈閉塞性疾患における脳酸素代謝とAcetazolamide反応性の関係について SPECT上のtype3はmisery perfusionを示唆するのか?
    黒田 敏, 宝金 清博, 志賀 哲, 加藤 千恵次, 玉木 長良, 岩崎 喜信, 阿部 弘 The Mt. Fuji Workshop on CVD 19 41 -44 2001年07月 [査読無し][通常論文]
     
    脳主幹動脈閉塞性疾患で,SPECTにてacetazolamide反応性を測定し,type 3と診断された症例を対象として比較検討した.PETパラメーターは,SPECTでtype 3と判定された各症例中で一様ではないことが判明した.即ち,rOEFが上昇している症例は14側中9側(64%),rCMRO2がほぼ正常域に保たれているのは6側,更に脳血管拡張が強く生じていることを示すrCBVの増加を伴うのは4側であった.一方,SPECTではtype 3と判定されたが,rOEFが正常範囲内であった例は14側中5側(36%)であった.いずれもrCMRO2は低下し,rCBFとrCMRO2との間に不均衡は存在していないと考えられた.又,rCMRO2の観点からみると,正常範囲内に保たれていた例は14側中6側(42.9%)であった
  • 上條 憲一, 宝金 清博, 喜友名 朝春, 高木 陽子, 山崎 敏正 臨床脳波 43 (7) 413 -418 2001年07月 [査読無し][通常論文]
     
    多チャネルの脳波データや脳磁場データから,脳内の主な活動源を推定する脳内等価電流双極子推定法は,空間的なデメリットを補う手法である.双極子推定法は,活動源のモデルである双極子モデルと頭部の電気的モデルを用いて,順問題及び逆問題を解くことによって行われる.双極子推定法をパソコンで行うソフトSynaPointProは,得られた推定結果をMRI上に重ね合わせたり,CGにより立体表示することができる.更に,推定結果の妥当性を評価するために,独自の評価基準や信頼領域を算出することができる.双極子推定法を癲癇の焦点推定に適用した事例から,臨床症状等の知見と一致することがわかった
  • K Houkin, S Kuroda, N Nakayama NEUROSURGERY CLINICS OF NORTH AMERICA 12 (3) 575 -+ 2001年07月 [査読無し][通常論文]
     
    Ninety-four patients with moyamoya disease (56 in the pediatric age group and 38 adults) were treated by revascularization surgery in the past 21 years (1979-2000). Combined surgery of the superficial temporal artery-middle cerebral artery (STA-MCA) anastomosis and indirect revascularization of encaphalo-duro-arterio-myo-synangiosis (EDAMS) was performed. Ischemic attacks disappeared in most patients within 1 year (mean) after surgery in pediatric cases. No morbidity or mortality was experienced in the pediatric group. Some children with cerebral infarction before the surgery, however, had mild mental retardation even after the surgery. Early diagnosis and proper prophylactic surgical treatment is indispensable for pediatric patients with moyamoya. MR angiography is an important diagnostic modality for the screening and longitudinal follow-up of moyamoya disease.
  • 七戸 秀夫, 宝金 清博, 黒田 敏, 石川 達哉, 阿部 弘, 牛越 聡, 布村 充, 会田 敏光 脳卒中の外科 29 (4) 255 -261 2001年07月 [査読無し][通常論文]
     
    過去15年間にわたる頸動脈内膜剥離術(CEA)をretrospectiveに解析し,冠動脈疾患の合併頻度について調べるとともに,CEAと冠動脈バイパス術(CABG)を同時に施行した4例(男3例・女1例,49〜72歳)について検討した.その結果,197例を対象とした16例(8.1%)に冠動脈疾患の合併が認められ,CEAのみ行った11例は術後53ヵ月後に死亡していた.同時手術については,その利点として入院期間短縮に伴う経費軽減化のほかに,CEAをCABGの直前に行うことにより人工心肺駆動中の血圧低下による脳虚血を避けることができ,CEA施行中に心機能が悪化してもすぐに人工心肺を駆動することができる点があった.短所としては手術時間の延長があったが,CEA中に大伏在静脈グラフトの採取を循環外科医に依頼するなどして短縮をはかれば解決できると考えられた
  • 黒田 敏, 宝金 清博, 阿部 弘 脳卒中の外科 29 (4) 272 -276 2001年07月 [査読無し][通常論文]
     
    脳血管撮影で,もやもや病と診断された女性32例(小児発症例)を対象に,妊娠や分娩に伴う問題点について検討した.その結果,小児期に発症し脳血行再建術を受けた2例が妊娠および出産を経験したが,いずれもMRAでは脳血行再建術による側副血行路の発達が良好で,全身麻酔下の帝王切開で娩出していた.以上のことからも,管理や分娩法については母親の神経症状や胎児の週齢に応じた麻酔法や娩出時期を選択する必要があると考えられた
  • H Aoyama, H Shirato, T Nishioka, K Kagei, R Onimaru, K Suzuki, S Ushikoshi, K Houkin, S Kuroda, H Abe, K Miyasaka RADIOTHERAPY AND ONCOLOGY 59 (3) 323 -328 2001年06月 [査読無し][通常論文]
     
    Background and purpose: We investigated the use of hypofractionated stereotactic radiotherapy (HFSR) to reduce adverse radiation effects in comparison to single-fraction stereotactic radiosurgery (SRS) for intracranial arteriovenous malformations (AVMs). Materials and methods: This study includes 53 intracranial AVMs treated between 1991-1998. HFSR was selected for 26 AVMs with a maximum diameter greater than or equal to2.5 cm or at eloquent area. Twenty-seven patients were treated with SRS (18 AVMs <2.5 cm at non-eloquent area, nine patients who were unlit for prolonged ring-wearing). The most frequent minimum dose (Dmin) was 20 Gy for SRS and 28 Gy for HFSR in four fractions. The mean follow-up duration was 34.6 months for SRS and 35.4 months for HFSR. Results: As a whole, the 3 and 5-year actuarial obliteration rates were 64 and 92%. Age <20 years old (P = 0.02) and a maximum diameter <2 cm were favorable factors (P = 0.05). A difference in the distribution of patients was observed in size (<greater than or equal to>2.5 cm or not) (P < 0.001) and location (eloquent or not) (P < 0.001) between SRS and HFSR due to the treatment selection. However, no significant differences were observed in the actuarial rates or obliteration and transient increased signals with T2-weighted MR images between SRS and HFSR. Radiation necrosis occurred in two patients treated with SRS and in none with HFSR. Intracranial hemorrhage after treatment happened in two treated with SRS and three with HFSR. Conclusions: HFSR appears to be at least us effective as SRS in achieving complete obliteration of intracranial AVM, although its definitive role remains to be investigated. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
  • 高橋 明弘, 石井 伸明, 浅岡 克之, 堀内 成好, 黒田 敏, 宝金 清博, Akihiro TAKAHASHI, Nobuaki ISHII, Katsuyuki ASAOKA, Naruyoshi HORIUCHI, Satoshi KURODA, Kiyohiro HOUKIN, Department of Neurosurgery Tokeidai Hospital, Department of Neurosurgery Tokeidai Hospital, Department of Neurosurgery Tokeidai Hospital, Department of Neurosurgery Tokeidai Hospital, Department of Neurosurgery Hokkaido University, Department of Neurosurgery Hokkaido University 脳卒中の外科 = Surgery for cerebral stroke 29 (3) 172 -177 2001年05月31日 [査読無し][通常論文]
     
    We describe 4 patients with spontaneous dissecting aneurysm of the intracranial verte bral artery, which presented with headache and neck pain as the only manifestation. The onset of headache and neck pain was gradual in all patients. Pains were distributed to the unilateral posterior head and neck region and were pulsating in 2 patients (50%) and steady in 2 patients (50%) and lasted for several minutes to several hours. The pains sometimes waxed and waned. The pains of the same kind appeared repeatedly. MRA was useful for detecting the vertebral artery abnormalities, such as string sign and aneurysmal dilatation. One patient was treated surgically because of refractory pain. The other patients were treated nonsurgically, i.e. bed rest and pain control. Follow-up MRA demonstrated healing of the dissection.
  • 様々な脳機能画像診断法 臨床的有用性の観点から
    宝金 清博 北海道医学雑誌 76 (3) 194 -194 2001年05月 [査読無し][通常論文]
  • 難波 理奈, 宝金 清博, 黒田 敏, 岩崎 喜信, 牛越 聡, 浅野 剛, 宮坂 和男 Neurological Surgery 29 (5) 393 -399 2001年05月 [査読無し][通常論文]
     
    脳動脈瘤の診断における3次元DSA(3D-DSA)の初期臨床経験について述べ,この新しい診断機器が今後占めると考えられる臨床的意義について検討した.3D-DSAは動脈瘤と母血管の関係を把握する上で非常に有用な検査であり,従来の2D-DSAに比べ,より明確に動脈瘤と血管の位置関係が描出された.また3D-CTAでは頭蓋底の骨によって視野が遮られる内頸動脈や前交通動脈瘤,アーチファクトの恐れがあるクリッピングやコイリング後の動脈瘤の観察においては,さらに有用である考えられた
  • 高橋 明弘, 石井 伸明, 浅岡 克之, 堀内 成好, 黒田 敏, 宝金 清博 脳卒中の外科 29 (3) 172 -177 2001年05月 [査読無し][通常論文]
     
    画像上,出血や梗塞を認めず症状が頭頸部痛のみの頭蓋内椎骨動脈解離性動脈瘤の4症例(37〜57歳,いずれも男性)を報告した.全例,頭頸部痛が徐々に出現し,2例は拍動性で2例は持続性で数分から数時間続き,しばしば増強および減弱を繰り返した.MR血管撮影では線状影や拡張した動脈瘤などの椎骨動脈異常を検出できた.1例は難治性の頭頸部痛のため手術治療を行い,他の3例は安静と疼痛管理からなる保存的治療を行った.フォローアップのMR血管撮影では血管壁の解離の治癒が認められた
  • K Kamada, F Takeuchi, K Houkin, M Kitagawa, S Kuriki, A Ogata, K Tashiro, Koyanagi, I, K Mitsumori, Y Iwasaki JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY 70 (5) 675 -678 2001年05月 [査読無し][通常論文]
     
    This case report describes a follow up investigation of a patient with impaired word discrimination due to mitochondrial encephalopathy with lactic acidosis and stroke-like syndrome (MELAS) using proton magnetic resonance spectroscopy (H-1 MRS) and auditory evoked magnetic fields (AEFs). The initial H-1 MRS showed no N-acetyl aspartate (NAA) and marked accumulation of lactate (Lac) in the strokelike lesion of MELAS, which M as silent in neural activity according to AEFs. The follow up investigations, however, demonstrated that NAA reappeared, that the formerly increased Lac signal was significantly reduced, and that the magnitude of AEFs of the lesion was markedly increased. Metabolic and functional changes in H-1 MRS and AEFs reflected the neurological recovery very well. The stroke-like lesion was shown, using AEFs and H-1 MRS, to be able to function properly, although brain tissue of the lesion initially had severe damage due to mitochondrial dysfunction.
  • K Kamada, F Takeuchi, T Harada, B Qiao, K Houkin, S Kuriki, Y Iwasaki, K Mitsumori NEUROREPORT 12 (6) 1297 -1300 2001年05月 [査読無し][通常論文]
     
    We recorded auditory evoked magnetic fields (AEFs) by presenting pure tone bursts once every 4000 ms in 11 patients with a brain lesion in or in the vicinity of the auditory cortex. AEFs on the damaged side revealed several enhanced deflections in late-latency AEFs (slow AEFs), peaking at similar to 320 (DI), 1030 (D2) and 1600 (D3) ms post-stimulus in eight patients. All the dipoles of slow AEFs were concentrated in the superior temporal regions which were not involved by brain lesions. D1, D2 and D3 dipoles were uniformly upward, downward and upward, respectively. The dipole moment varied from 12 to 122 nAm and had no consistent relationship with latency. This is the first report describing slow AEFs in cases with temporal lobe lesions. NeuroReport 12:1297-1300 (C) 2001 Lippincott Williams & Wilkins.
  • R Nanba, K Houkin, S Kuroda, Y Iwasaki, S Ushikoshi, T Asano, K Miyasaka NEUROLOGICAL SURGERY 29 (5) 393 -399 2001年05月 [査読無し][通常論文]
     
    Recent rapid advancement in the technology of magnetic resonance angiography and 5-dimensional computed tomography angiography has opened the door to a less-invasive diagnostic routine for the treatment of cerebral aneurysms. However, from the viewpoint of decision making concerning which treatment to use; conservative therapy; surgical intervention; or endovascular treatment, much more precise information is necessary. Recent progress of rotation digital subtraction angiography has introduced 3-dimensional digital subtraction angiography. In the past year, 28 cases involving 39 aneurysms have been studied using S-dimensional angiography. This study was carried out, using a rotating C-arm, which rotates 220 degrees in 5.8 seconds. Air raw data gathered was transferred to the workstation for image reconstruction. The purpose of this study was to evaluate the clinical potential of 3-dimensional digital subtraction angiography for the diagnosis of the cerebral aneurysms.
  • 鎌田 恭輔, 竹内 文也, 栗城 真也, 宝金 清博, 岩崎 喜信 臨床神経生理学 29 (2) 171 -171 2001年04月 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 10 (4) 250 -250 2001年04月 [査読無し][通常論文]
  • 喬 梵, 鎌田 恭輔, 黒田 敏, 宝金 清博, 岩崎 喜信 脳卒中 23 (1) 77 -77 2001年03月 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博, 安田 宏, 小林 徹, 新谷 好正, 岩崎 喜信, 阿部 弘 脳卒中 23 (1) 117 -117 2001年03月 [査読無し][通常論文]
  • 鎌田 恭輔, 三森 研自, 宝金 清博, 岩崎 喜信 脳卒中 23 (1) 126 -126 2001年03月 [査読無し][通常論文]
  • 七戸 秀夫, 黒田 敏, 宝金 清博, 岩崎 慶信, 布村 充 脳卒中 23 (1) 136 -136 2001年03月 [査読無し][通常論文]
  • 宝金 清博 Pharma Medica 19 (2) 147 -150 2001年02月 [査読無し][通常論文]
     
    血行再建術後も脱力発作や頭痛等の脳虚血症状が緩解しない小児もやもや病の5例に対してニルバジピンを使用した.使用に伴う血圧・脈拍の変化はみられず,血液生化学所見にも変動はみられなかった.使用後,4例で症状が消失・軽快したが,1例には無効であった.無効例の検討により,血行再建術の効果が低い症例において本剤の効果はあまり期待できないことが示唆された
  • 救急医療 頭部外傷
    宝金 清博 北海道医報 (963) 8 -12 2001年01月 [査読無し][通常論文]
  • 中山若樹, 宝金清博, 黒田敏 ウィリス動脈輪閉塞症の病因・病態に関する研究班 平成12年度総括・分担研究報告書 35-44 -44 2001年 [査読無し][通常論文]
  • 鎌田 恭輔, Kober Helmut, 寶金 清博 臨床脳波 = Clinical electroencephalography : 脳波・筋電図と臨床神経生理 43 (1) 29 -36 2001年01月 [査読無し][通常論文]
  • もやもや病の治療
    神経内科 54 340 -347 2001年 [査読無し][通常論文]
  • 井戸坂 弘之, 黒田 敏, 宝金 清博, 阿部 弘, 志賀 哲, 玉木 長良 Neurological Surgery 29 (1) 45 -50 2001年01月 [査読無し][通常論文]
     
    脳動静脈奇形2例の術前診断に,C15 O2 gasと18F-FDPの各々によるPETを用いて脳賦活試験を行い運動野の同定を試みた.その結果,MRIとの合成画像を作成することによって機能局在を確認でき,治療方針決定に有用であった
  • N Nakayama, S Kuroda, K Houkin, S Takikawa, H Abe ACTA NEUROCHIRURGICA 143 (1) 17 -24 2001年 [査読無し][通常論文]
     
    Background. The purpose of this study was to examine the utility and reliability of arterial flow measurements made with a transit time ultrasonic flowmeter for monitoring blood flow changes during intracranial and carotid surgery. Method. A total of 25 patients underwent intra-operative arterial blood flow measurements. The pulsatile flow curve and mean flow values were obtained using 1- to 6-mm transit time probes with a dual channel flowmeter. Four cases underwent aneurysm clipping, 11 cases superficial temporal artery (STA) - middle cerebral artery (MCA) bypass, 2 cases external carotid artery (ECA)- radial artery - MCA bypass for aneurysm trapping, and 8 cases carotid endarterectomy. In aneurysm clipping, blood flow in the branches distal to the aneurysm was measured before and after clipping. Blood flow in the STA was measured before and after STA-MCA anastomosis, and blood flow in the internal carotid artery (ICA) cervical portion was measured during carotid endarterectomy. Blood flow in the MCA and STA was monitored during radial artery grafting. Findings. Blood flow in the STA was elevated after STA-MCA anastomosis. However, post-operative hyperperfusion syndrome was found in some cases whose flow elevation was over 50 ml/min. Also in one case of carotid stenosis. of which blood flow of ICA was elevated to 400 ml/min after carotid endarterectomy, hyperperfusion syndrome was found after surgery. In the cases of MCA aneurysm clipping, decreasing of M2 flow was detected when clipping caused bifurcation stenosis. Interpretation. We found transit time flow measurement useful for management of cerebrovascular surgery: the technique was simple to use and provided stable, reliable results. The method was able to reveal distal branch flow diminution in aneurysm clipping, or residual flow during temporary clipping in aneurysm surgery, and has the potential to predict post-operative complications such as hyperperfusion by signalling over-elevation of donor artery flow in bypass surgery or ICA flow in carotid surgery.
  • Satoshi Kuroda, Kiyohiro Houkin, Hiroyasu Kamiyama, Hiroshi Abe Neurosurgery 49 (2) 463 -468 2001年 [査読無し][通常論文]
     
    OBJECTIVE AND IMPORTANCE: The beneficial effects of surgical revascularization on rebleeding in moyamoya disease remain unclear. This report is intended to clarify the effects of surgical revascularization on peripheral artery aneurysms, which represent one of the causes of intracranial bleeding in moyamoya disease. CLINICAL PRESENTATION: Findings for three female patients who experienced intracranial bleeding are presented. Cerebral angiography revealed that intracranial bleeding resulted from the rupture of peripheral artery aneurysms arising from dilated collateral vessels such as the lenticulostriate artery. INTERVENTION: The patients successfully underwent superficial temporal artery-middle cerebral artery anastomosis combined with encephaloduromyoarteriosynangiosis. Angiography demonstrated obliteration of the peripheral artery aneurysms, together with the disappearance or decrease in caliber of the parent collateral arteries, after surgery. None of the patients experienced rebleeding during the follow-up period (up to 52 mo). CONCLUSION: The results strongly suggest that surgical revascularization potentially improves cerebral circulation and decreases hemodynamic stress on collateral vessels, obliterating peripheral artery aneurysms.
  • Management Strategies: Pregnant Patient
    The American Association of Neurological Surgeons (AANS) Topic Book. "Moyamoya Disease". 2001年 [査読無し][通常論文]
  • Chapter 12. Direct bypass suregery
    The American Association of Neurological Surgeons (AANS) Topic Book. "Moyamoya Disease 2001年 [査読無し][通常論文]
  • Chapter 11, Part 2. Management Strategies: Selection of Surgical Procedures, Outcome, ischemic vs. hemorrhagic forms, adults vs. children
    The American Association of Neurological Surgeons (AANS) Topic Book. "Moyamoya Disease" 2001年 [査読無し][通常論文]
  • Chapter 8. Magnetic Resonance Image and CT scan Diagnosis
    The American Association of Neurological Surgeons (AANS) Topic Book. "Moyamoya Disease". 2001年 [査読無し][通常論文]
  • 中山若樹, 宝金清博, 斎藤久寿 日本脳循環代謝学会総会プログラム・抄録集 12th 84 2000年12月05日 [査読無し][通常論文]
  • 難波 理奈, 黒田 敏, 宝金 清博, 牛越 聡, 宮坂 和男 新潟医学会雑誌 114 (12) 499 -499 2000年12月
  • 鎌田 恭輔, 原田 達男, 宝金 清博, 阿部 弘, 竹内 文也, 栗城 真也, 三森 研自 新潟医学会雑誌 114 (12) 496 -496 2000年12月 [査読無し][通常論文]
  • Magnetoencephalography(MEG)を用いたローランドてんかんの神経活動の分析
    鎌田 恭輔, 宝金 清博, 阿部 弘 てんかんをめぐって XX 106 -107 2000年12月 [査読無し][通常論文]
  • 難波 理奈, 黒田 敏, 宝金 清博, 牛越 聡, 宮坂 和男 新潟医学会雑誌 114 (12) 499 -499 2000年12月 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博, 上山 博康, 池田 潤, 三森 研自, 能條 建, 斉藤 久壽, 柏葉 武, 布村 充, 岩崎 喜信, 阿部 弘 脳卒中 22 (4) 657 -660 2000年12月 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博, 石川 達哉, 高橋 明弘, 上山 博康, 中川 翼, 阿部 弘 脳卒中の外科 28 (6) 421 -426 2000年11月 [査読無し][通常論文]
     
    過去20年間に経験した14歳未満で発症した小児もやもや病51例を対象とし,長期予後について,初発症状,手術時期,手術方法,脳虚血発作の推移,脳循環動態,知能,就学および就職状況など幅広い点から検討した.男児21例,女児30例であり,発症は0〜13歳で,1例を除いた50症例95半球に対して,1〜29歳時に脳血行再建術がなされた.1979〜1985年には26側半球に対して,側頭部を中心とするencephalo-myo-arterio-synangiosis(EMAS)等の間接的血行再建術が主に行われた.1985〜1998年には69側に対して,前頭側頭部を中心とするSTA-MCA anastomosis+encephalo-duro-arterio-myo-synangiosis(EDAMS)等の直接・間接的血行再建術の併用が主に行われた.術式の違いにより差異は認められたものの,脳血行再建術によりTIAの頻度は明らかに減少し,殆どの症例で消失することが確認されたTIA型症例では発症後まもないうちに早期診断を行い,できる限り早い時期に脳血行再建術を実施する必要性を示唆していた
  • 総頸動脈,鎖骨下動脈,椎骨動脈閉塞性病変に対する外科治療の現状と展望
    宝金 清博, 黒田 敏, 阿部 弘 日本脳神経外科学会総会抄録集 59回 117 -117 2000年10月 [査読無し][通常論文]
  • 視力障害で発症した頸動脈閉塞性病変の検討
    小林 徹, 黒田 敏, 宝金 清博, 斉藤 久寿 日本脳神経外科学会総会抄録集 59回 201 -201 2000年10月 [査読無し][通常論文]
  • 頭蓋内硬膜動静脈瘻に対する外科的治療
    牛越 聡, 宝金 清博, 黒田 敏, 浅野 剛, 宮坂 和男 日本脳神経外科学会総会抄録集 59回 55 -55 2000年10月 [査読無し][通常論文]
  • 脳磁図及びProton MRSによる聴皮質機能回復及び再構築の検討
    鎌田 恭輔, 竹内 文也, 原田 達男, 北川 道生, 栗城 真也, 三森 研自, 宝金 清博 日本脳神経外科学会総会抄録集 59回 102 -102 2000年10月 [査読無し][通常論文]
  • 乳幼児もやもや病の病態と脳血行再建術
    黒田 敏, 宝金 清博, 上山 博康, 阿部 弘 日本脳神経外科学会総会抄録集 59回 140 -140 2000年10月 [査読無し][通常論文]
  • もやもや病に対する脳血行再建術は再出血のリスクを減らせるか?
    黒田 敏, 宝金 清博, 上山 博康, 阿部 弘, 斉藤 久寿, 三森 研自, 柏葉 武, 布村 充 日本脳神経外科学会総会抄録集 59回 297 -297 2000年10月 [査読無し][通常論文]
  • 瀧川 修吾, 宝金 清博, 板本 孝治, 黒田 敏, 中山 若樹, 斎藤 久寿 日本脳神経外科学会総会抄録集 59回 (Abstract) 165 -165 2000年10月 [査読無し][通常論文]
  • 中山 若樹, 黒田 敏, 宝金 清博, 瀧川 修吾, 斎藤 久壽, 阿部 弘 日本脳神経外科学会総会抄録集 59回 (Abstract) 203 -203 2000年10月 [査読無し][通常論文]
  • 【画像診断の進歩 神経内科疾患への応用】磁気共鳴スペクトロスコピーの臨床応用
    鎌田 恭輔, 宝金 清博 神経内科 53 (3) 227 -240 2000年09月 [査読無し][通常論文]
  • 大西 晶子, 宝金 清博, 数又 研, 黒田 敏, 阿部 弘, 会田 敏光, 布村 充, 斉藤 久寿, 上山 博康, 宮町 敬吉 脳卒中の外科 28 (5) 345 -349 2000年09月 [査読無し][通常論文]
     
    外科治療を受けた妊娠合併脳卒中12例において,平均年齢は29.5歳,発症時妊娠週数は26週であった.原因疾患は動静脈奇形の出血4例,もやもや病の出血3例,くも膜下出血2例,解離性動脈瘤による脳梗塞1例,妊娠中毒症による高血圧性脳内出血2例であった.治療の結果,母体は7例が後遺症を残さず転帰良好であったが,後遺症を4例に認め,1例は死亡した.児では7例が転帰良好であったが,2例は先天性心疾患のため生後まもなく死亡した.また原因不明の母体内死亡が2例あり,中絶が1例であった.原因疾患別の母体側転帰では,動静脈奇形,もやもや病,高血圧性脳内出血の9例では7例で後遺症がなく,2例の後遺症もごく軽度であったが,くも膜下出血,脳梗塞例では死亡1例,重篤な後遺症2例と転帰不良であった
  • 黒田 敏, 宝金 清博, 小林 徹, 安田 宏, 牛越 聡, 斎藤 久寿, 阿部 弘 Neurological Surgery 28 (7) 607 -613 2000年07月 [査読無し][通常論文]
     
    HEO-200を用い,頸動脈遮断中のモニタリングを行った24例を対象に検討した.その結果,全例で安定したNIRSのシグナルを検出可能であった.しかし,SEPでは2例で電気的なアーチファクトが原因で測定不能であった.またTCDの測定は17例で試みたが,うち4例で測定できなかった.CEAの際に外頸動脈を遮断すると,頭皮上から得られるNIRSにおいて,一部,tHbが減少することから頭皮上でもモニタリングする際に一部頸動脈系シグナルも混在することがHEO-200でも確認した.したがってCEAの際には頭皮成分の混入を少なくする等の工夫をし,脳内変化の区別を検出できるように工夫が必要である
  • S Kuroda, K Houkin, T Kobayashi, H Yasuda, S Ushikoshi, H Saito, H Abe NEUROLOGICAL SURGERY 28 (7) 607 -613 2000年07月 [査読無し][通常論文]
     
    Near infrared spectroscopy (NIRS) have been accepted as a useful modality for non-invasive monitoring of brain oxygenation state. Using a newly developed NIRS apparatus, HEO-200, the authors continuously monitored the changes in the oxygenation state of brain hemoglobin during carotid endarterectomy (CEA) or carorid balloon occlusion tests (BOT) in 21 patients. Somatosensory evoked potentials (SEP),regional cerebral blood flow (rCBF) or transcranial Doppler sonography (TCD) were also employed to compare with the NIRS-responses. Tn 7 of 21 patients, the carotid artery occlusion caused a continuous deoxygenation of brain hemoglobin as well as a decrease in total hemoglobin. These findings correlated well with temporary neurological deficits and a marked reduction of rCBF in the ipsilateral hemisphere in patients who underwent carotid BOT under local anesthesia. The patients also showed a marked decrease in the N20 amplitude of SEP during CEA under general anesthesia. Restoration of blood now immediately resolved these findings. The other 14 patients demonstrated no or only transient changes on NIPS as well as on SEP and rCBF study. HEO-200 could non-invasively monitor the relative changes of cerebral oxygenation state with good time resolution, and detect critical ischemia during CEA and carotid BOT.
  • KURODA Satoshi, HOUKIN Kiyohiro, NUNOMURA Mitsuru, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 40 (6) 315 -320 2000年06月15日 [査読無し][通常論文]
     
    A 60-year-old female and a 40-year-old male underwent surgical revascularization for moyamoya disease and suffered small infarction in the ipsilateral frontal lobe 3 or 4 days postoperatively. Neuroimaging suggested that the bypass flow had caused rapid progression of occlusive changes in the carotid forks, a diminishing of moyamoya vessels, and flow reduction in the anterior cerebral artery ipsilateral to surgery, leading to critical ischemia in the frontal lobe. Surgical revascularization improves the outcome of patients with moyamoya disease, but postoperative management such as hydration is important to avoid ischemic complications due to frontal lobe infarction.
  • K Houkin TECHNIQUES IN NEUROSURGERY 6 (2) 164 -171 2000年06月 [査読無し][通常論文]
     
    Indirect revascularization is an important surgical technique for the treatment of moyamoya disease. This technique can be applied to other forms of cerebral ischemia, though at present. it is used exclusively for revascularization in moyamoya disease. The donor material is vascular rich tissue that can potentially induce neovascularization of the ischemic brain, and as the donor site, the temporal muscle, other muscles, the dura mater, the middle cerebral artery, the superficial temporal and occipital arteries, and the omentum can be used. However, the success of this indirect revascularization is mostly dependent on the condition of the recipient (brain). Cytokines, including basic fibroblast growth factor, may play an important role in neovascularization. The authors relate their experience with indirect revascularization in patients with moyamoya disease and review this indirect revascularization method, focusing in detail on the surgical technique.
  • 黒田 敏, 宝金 清博, 能條 建, 池田 潤, 上山 博康, 斎藤 久寿, 三森 研自, 柏葉 武, 阿部 弘 脳卒中 22 (1) 199 -199 2000年04月 [査読無し][通常論文]
  • 七戸 秀夫, 黒田 敏, 宝金 清博, 阿部 弘, 菊池 陽一, 牛越 聡 脳卒中 22 (1) 282 -282 2000年04月 [査読無し][通常論文]
  • 小林 徹, 黒田 敏, 多田 光宏, 宝金 清博, 阿部 弘 脳卒中 22 (1) 208 -208 2000年04月 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博, 斎藤 久寿, 三森 研自, 柏葉 武, 阿部 弘 脳卒中 22 (1) 141 -141 2000年04月 [査読無し][通常論文]
  • 鎌田 恭輔, 宝金 清博, 阿倍 弘 臨床神経生理学 28 (2) 131 -131 2000年04月 [査読無し][通常論文]
  • T Yamauchi, M Tada, K Houkin, T Tanaka, Y Nakamura, S Kuroda, H Abe, T Inoue, K Ikezaki, T Matsushima, M Fukui STROKE 31 (4) 930 -935 2000年04月 [査読無し][通常論文]
     
    Background and Purpose-Moyamoya disease is a cerebrovascular disease of unknown cause that mainly affects Japanese children. The incidence of familial occurrence accounts for 9% of cases. The characteristic lesions of moyamoya disease are occasionally seen in neurofibromatosis type I, of which the causative gene (NF1) has been assigned to chromosome 17q11.2. Methods-To determine whether a gene related to moyamoya disease is located on chromosome 17, we conducted microsatellite linkage analyses on 24 families containing 56 patients with moyamoya disease, Leukocyte DNA extracted from the family members was subjected to polymerase chain reaction for a total of 22 microsatellite markers on chromosome 17. The amplified polymerase chain reaction fragments were analyzed with GeneScan on an automated sequencer. Results-Two-point linkage analysis gave a maximum log,, odds (LOD) score of 3.11 at the recombination fraction of 0.00 for the marker at locus D17S939. The affected pedigree member method also showed a significantly low P value (<1.0X10(-5)) far the 5 adjacent markers at 17q25. Multipoint linkage analysis also indicated that the disease gene is contained within the 9-cM region of D17S785 to D17S836, with a maximum LOD score of 4.58. Conclusions-A gene for familial moyamoya disease is located on chromosome 17q25.
  • 両側頸動脈狭窄を伴う冠動脈3枝病変に対する同時手術の経験
    佐藤 浩之, 松居 喜郎, 山内 英智, 上久保 康弘, 椎谷 紀彦, 村下 十志文, 佐々木 重幸, 佐久間 まこと, 安田 慶秀, 宝金 清博 The Japanese Journal of THORACIC AND CARDIOVASCULAR SURGERY 48 (Suppl.) 4 -4 2000年03月 [査読無し][通常論文]
  • 宝金 清博, 黒田 敏, 阿部 弘 脳卒中の外科 28 (2) 115 -120 2000年03月 [査読無し][通常論文]
     
    1)もやもや病における脳血行再建のdonorは,3本の動脈(浅側頭動脈,深側頭動脈,中硬膜動脈)であり,これらを最大限に利用する術式が重要である. 2)術前に外頸動脈の側面像と頭部側面像(デジタル画像)を撮り,これを合成した画像を作成しておくと,これらの動脈の走行を術前に十分予測した手術が可能である.又,個々の症例に応じた術式を行うべきである. 3)術前の血管撮影に基づいたEDAMSと直接的血行再建術は,これらの要件を満たす術式である
  • 中山若樹, 宝金清博, 黒田敏 ウィリス動脈輪閉塞症の病因・病態に関する研究班 平成11年度研究報告書 29-36 -36 2000年 [査読無し][通常論文]
  • 小児もやもや病の術後長期成績--当科における20年間の経験から
    脳卒中の外科 28 114 -119 2000年 [査読無し][通常論文]
  • 外科解剖に基づいたもやもや病に対する血行再建術.
    脳卒中の外科 28 114 -119 2000年 [査読無し][通常論文]
  • Chapter 11 外科技術のデジタルアーカイブ
    脳血行再建術 2000年 [査読無し][通常論文]
  • Chapter 8 椎骨動脈、鎖骨下動脈再建術
    脳血行再建術 2000年 [査読無し][通常論文]
  • Chapter 6 橈骨動脈を用いたlong graft bypass
    脳血行再建術 2000年 [査読無し][通常論文]
  • Chapter 5 間接的血行再建
    脳血行再建術 2000年 [査読無し][通常論文]
  • Chapter 2 基本練習
    脳血行再建術 2000年 [査読無し][通常論文]
  • Chapter 7 内頚動脈血栓内膜剥離術
    脳血行再建術 2000年 [査読無し][通常論文]
  • Chapter 10 モニタリング
    脳血行再建術 2000年 [査読無し][通常論文]
  • Chapter 9 静脈血行再建
    宝金清博著, 上山博康監修 2000年 [査読無し][通常論文]
  • K Houkin, S Kuroda JOURNAL OF NEUROSURGERY 92 (1) 176 -180 2000年01月 [査読無し][通常論文]
     
    The authors examine the quality of intraoperative photography in which digital recording technology, including a microdigital camera and digital video paired with an operating microscope, is used during neurosurgery. A microdigital camera developed for this purpose (1.4 million pixels) was attached to an operating microscope and used during surgery. The same surgical views with precisely the same optical conditions were taken through the microscope by using both a conventional 35-mm camera and the microdigital camera, and the quality of the final output was compared. In addition, the quality of the digital camera photographs was compared with the still photograph clipped from the digital video recording. The quality of the photographs taken with a microdigital camera was superior to the quality of those obtained with the conventional 35-mm camera. The success rate of recording (what you see is what you get) was almost 100%. The quality of the still photographs clipped from the digital video was nearly equal to those taken with the digital camera. The microdigital camera system is superior to the conventional 35-mm camera in neurosurgery in terms of its success rate and the quality of the photography. It is also a space-saving system for storing the huge amount of data generated in the recording of surgical procedures, and the cost/performance ratio is superior to that of the conventional method. Digital technology including digital cameras and videos is very useful for clear recording of microsurgical procedures.
  • Kiyohiro Houkin, S. Kuroda, T. Ishikawa, H. Abe Acta Neurochirurgica 142 (3) 269 -276 2000年 [査読無し][通常論文]
     
    The effects of direct and indirect revascularization for moyamoya disease were analyzed for each donor artery to determine which surgical procedure is most useful for the induction of neovascularization. In the past 12 years, 85 patients with moyamoya disease were surgically treated by combined surgery consisting of indirect revascularization via encephalo-duro-arterio-myo-synangiosis (EDAMS) and direct revascularization via the superficial temporal artery and the middle cerebral artery (STA-MCA) bypass. Among those patients, the post-operative changes in digital subtraction angiography were examined in 56 sides, including 34 sides in paediatric cases and 22 sides in adult cases. The neovascularization after indirect revascularization using the 1) superficial temporal artery (skin), 2) middle meningeal artery (dura mater), 3) deep temporal artery (temporal muscle) was analyzed. As results, in paediatric cases, the deep temporal artery and middle meningeal artery induced good neovascularization. However, the induction of neovascularization from the superficial temporal artery was not always good in most pediatric and adult cases. On the other hand, the direct bypass was useful in 90% of adult cases. In indirect revascularization surgery for moyamoya disease, the temporal muscle (the deep temporal artery) and the dura mater (the middle meningeal artery) are useful donors to the ischemic brain. The simple encephalo-arterio-synangiosis is not always effective. The direct bypass is a useful technique for adult moyamoya disease.
  • Neurol Med Chir (Tokyo) 40 (6) 315 -320 2000年 [査読無し][通常論文]
  • K Houkin, S Kuroda, H Abe ACTA NEUROCHIRURGICA 142 (8) 913 -919 2000年 [査読無し][通常論文]
     
    Background. The purpose of this study was to develop a new method for more efficient and accurate operative records using intraoperative digital data in neurosurgery, including macroscopic procedures and microscopic procedures under an operating microscope. Methods. Macroscopic procedures were recorded using a digital camera and microscopic procedures were also recorded using a microdigital camera attached to an operating microscope. Operative records were then recorded digitally and filed in a computer using image retouch software and database base software. Findings. The time necessary for editing of the digital data and completing the record was less than 30 minutes. Once these operative records are digitally filed, they are easily transferred and used as database. Using digital operative records along with digital photography, neurosurgeons can document their procedures more accurately and efficiently than by the conventional method (handwriting). Interpretation. A complete digital operative record is not only accurate but also time saving. Construction of a database, data transfer and desktop publishing can be achieved using the intra-operative data, including intra-operative photographs.
  • Clinical application of near-infrared spectroscopy (NIRS) to cerebrovascular disorders.
    Neuroscience and Music 2000年 [査読無し][通常論文]
  • Cerebrovascular Disease: Ischemia, a. Intracranial Lesio
    脳神経外科レビュー（8） 2000年 [査読無し][通常論文]
  • 七戸 秀夫, 黒田 敏, 石川 達哉, 宝金 清博, 阿部 弘, 松居 喜郎, 牛越 聡, 菊池 陽一 新潟医学会雑誌 113 (11〜12) 575 -575 1999年12月 [査読無し][通常論文]
  • 高橋 明弘, 石井 伸明, 宝金 清博, 黒田 敏 新潟医学会雑誌 113 (11〜12) 547 -548 1999年12月 [査読無し][通常論文]
  • 鎌田 恭輔, 宝金 清博, 黒田 敏, 阿部 弘, 三森 研自 新潟医学会雑誌 113 (11〜12) 558 -558 1999年12月 [査読無し][通常論文]
  • 宝金 清博, 黒田 敏, 阿部 弘, 瀧川 修吾, 斎藤 久寿 Neurological Surgery 27 (12) 1111 -1118 1999年12月 [査読無し][通常論文]
     
    デジタル画像は,手術顕微鏡下の記録としても非常に優れている.静止画像では,マイクロデジタルカメラにより,アナログカメラに較べ,遥かに高い画質と安定した撮影が可能である.動画では,画質の点では,ハイビジョン方式が優れている.汎用性や費用の面を考慮すると,現時点では,デジタルビデオが最も実用的であり,外科医が満足する画質が適切なコストで得られる
  • K Houkin, S Kuroda, H Abe, S Takikawa, H Saitoh NEUROLOGICAL SURGERY 27 (12) 1111 -1118 1999年12月 [査読無し][通常論文]
     
    The purpose of this study is to examine the image quality of multi-media far digital recording of surgical procedure using an operating microscope in neurosurgery. For video recording, high vision recording, digital video recording and analogue VHS recording were used. For still pictures, an analogue camera with 35mm silver halide film and a micro-digital camera were used. The quality of photographs taken with a microdigital camera was superior to the quality of those taken with the conventional 35 mm film camera. The micro-digital camera system is superior to the conventional 35 mm camera in neurosurgery in its quality and success rate. In video recording, high vision analogue recording was superior to any other media as far as its image quality is concerned although its practical convenience is limited and cost performance is not always good. On the other hand, digital video can record high quality images, including still pictures, with satisfactory quality to the neurosurgeon. These digital recording media are also space saving for storing the huge amount of data obtained during surgery and the cost-performance is superior to that of the conventional method. In the near future, most of surgical procedures are supposed to be expected using digital media.
  • 宝金 清博, FUKUHARA Shunichi, SELLADURAI Benedict, RAHMAN Adnan Abd Rahman, ISHAK Muin, KURODA Satoshi, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 39 (11) 773 -788 1999年10月15日 [査読無し][通常論文]
     
    デジタル国際電話同義を用いた画像転送、遠隔会議システムを日本とマレーシア間に構築した。このシステムは、通常の個人用コンピューターとイメージスキャナー、ターミナルアダプターから構成されている。転送された画像は、診断と討論に寄与する高い質を有していた。ただ，単純写真のようなデータ量が多いものでは、質の低下が見られた。画像の一枚の転送には約20秒を要し、これにかかる費用は、郵送とほぼ同等であった。このシステムの最も大きな特徴は、その高い画質と経済的効率性、そして、プライバシーの保護性の高さである。このシステムにより、先進国と円売途上国の脳神経外科医の不均等分布の問題の一つの序次の方向性が明らかとなった。
  • 小児もやもや病の術後長期成績 脳虚血発作,進学状況,脳循環動態の検討
    黒田 敏, 宝金 清博, 上山 博康, 中川 翼, 阿部 弘 日本脳神経外科学会総会抄録集 58回 57 -57 1999年10月 [査読無し][通常論文]
  • モヤモヤ病家系におけるMicrosatellite連鎖解析-第17番染色体での検討
    山内 亨, 多田 光宏, 宝金 清博, 黒田 敏, 阿部 弘, 福井 仁士, 中村 祐輔, 柏葉 武 日本脳神経外科学会総会抄録集 58回 58 -58 1999年10月 [査読無し][通常論文]
  • 破裂動脈瘤術後の髄液ドレナージ過剰症候群
    鎌田 恭輔, 宝金 清博, 黒田 敏, 野村 三起夫, 瀧川 修吾, 斎藤 久寿, 三森 研自, 阿部 弘 日本脳神経外科学会総会抄録集 58回 313 -313 1999年10月 [査読無し][通常論文]
  • Neck clipping困難な動脈瘤に対するGDCを用いた母動脈閉塞
    牛越 聡, 菊池 陽一, 宮坂 和男, 宝金 清博, 黒田 敏, 阿部 弘 日本脳神経外科学会総会抄録集 58回 358 -358 1999年10月 [査読無し][通常論文]
  • 小林 徹, 黒田 敏, 中村 俊孝, 中山 若樹, 宝金 清博, 阿部 弘 日本脳神経外科学会総会抄録集 58回 (Abstract) 257 -257 1999年10月 [査読無し][通常論文]
  • 瀧川 修吾, 宝金 清博, 黒田 敏, 中山 若樹, 斎藤 久寿, 阿部 弘 日本脳神経外科学会総会抄録集 58回 (Abstract) 306 -306 1999年10月 [査読無し][通常論文]
  • 中山 若樹, 宝金 清博, 黒田 敏, 瀧川 修吾, 斉藤 久寿, 阿部 弘 日本脳神経外科学会総会抄録集 58回 (Abstract) 121 -121 1999年10月 [査読無し][通常論文]
  • 手術記録の新しい書き方(デジタル記録の応用)
    宝金 清博, 黒田 敏 脳神経外科速報 9 (10) 805 -809 1999年10月 [査読無し][通常論文]
  • K Houkin, S Fukuhara, BM Selladurai, AA Zurin, M Ishak, S Kuroda, H Abe NEUROLOGIA MEDICO-CHIRURGICA 39 (11) 773 -777 1999年10月 [査読無し][通常論文]
     
    A new image transmission and teleconference system using international digital telephone services was established between Japan and Malaysia. This new system consists of an ordinary personal computer, image scanner, and terminal adapter for digital telephone lines. The quality of images transferred using this system was high enough for diagnosis and discussion except for images such as radiographs requiring huge data transfer. Transmission of one image took approximately 20 seconds. The cost performance was almost equal to the conventional mailing system. The most remarkable advantage of this new system is the high quality of transferred images, the cost and time performance, and security of the medical information. New communication systems using international digital networks including the internet may allow re-distribution of medical resources between advanced countries and developing countries in neurosurgery.
  • 瀧川 修吾, 宝金 清博 綜合臨床 48 (8) 1878 -1880 1999年08月 [査読無し][通常論文]
  • T Kobayashi, K Houkin, F Ito, Y Kohama NEUROSURGERY 45 (2) 299 -302 1999年08月 [査読無し][通常論文]
     
    OBJECTIVE: We present two cases of common carotid artery occlusion that were treated by vascular reconstruction using the transverse cervical artery. METHODS: Two patients with common carotid artery occlusion presented with transient ischemic attacks resulting from decreased cerebral blood flow on the affected side. Both patients underwent vascular reconstruction using the transverse cervical artery. The transverse cervical artery was anastomosed to the ipsilateral external carotid artery at its origin, as a pedicle graft. A superficial temporal artery-middle cerebral artery anastomosis was then performed. RESULTS: The postoperative courses were uneventful. The transverse cervical artery bypass grafts were patent, and cerebral blood flow increased to normal levels. CONCLUSION: Transverse cervical artery grafting provides a less tedious alternative to saphenous vein interposition grafting for revascularization of the internal carotid artery domain.
  • K Kazumata, S Fujimoto, H Idosaka, S Kuroda, M Nunomura, K Houkin AMERICAN JOURNAL OF NEURORADIOLOGY 20 (7) 1243 -1244 1999年08月 [査読無し][通常論文]
     
    A case of multiple cerebral varices located in the superficial cerebral veins and ipsilateral internal jugular vein is reported.
  • 宝金 清博, 鎌田 恭輔 核医学 36 (6) 530 -530 1999年08月 [査読無し][通常論文]
  • 神経活動に伴う光SignalsとFlow Oscillation由来の光Signals(第2報)
    根本 正史, 能條 建, 宝金 清博, 田村 守, 阿部 弘 脳循環代謝 11 (1) 65 -66 1999年07月 [査読無し][通常論文]
     
    Rat大脳皮質において,体性感覚刺激時に観測される賦活部での機能的光signalsと,flow oscillation由来の光signalsについて,両側頭頂部にthinned skull windowを作製,左右大脳皮質を同一視野において,これらの光signalsがどのような左右の連関をもって生じているのか観察した.機能的光signalsは,刺激反対側の賦活皮質で認められたが,同側皮質にも,強度は弱いながら類似したtime courseと波長依存性をもつsignalsが,同期して,観察された.これらの変化は,分光学的計測からhemoglobin(Hb)の局所濃度と酸素化状態の変化に対応し,神経活動に伴う代謝と血流の応答を示唆していた
  • ラット慢性脳虚血モデルを用いた脳循環動態の検討
    能條 建, 田村 守, 佐藤 知絵, 根本 正史, 宝金 清博, 黒田 敏, 阿部 弘 脳循環代謝 11 (1) 24 -25 1999年07月 [査読無し][通常論文]
     
    ラットの一側頸動脈-頸静脈短絡モデルを用いて,分光学的手法により脳循環動態について検討を加えた.短絡側の脳表細動脈は対側に比し拡張しCO2吸入に伴う血管拡張能も障害されていたが,短絡結紮後,血管径は正常化し血管反応性も改善した.CO2吸入に伴うoxyHb,TotalHb(CBV)の増加反応は短絡側で遅延しその反応も軽度であったが,短絡結紮後,潜時は短縮し,変化も増大傾向を示した.短絡結紮によりoxyHbは一過性に急上昇し,やや前値に復するも上昇を維持した.これはLDFにより観察されたCBFの挙動と近似していた.逆に,短絡結紮によりCBV(TotalHb)は漸減した.脳内のHb酸素飽和度は短絡側で低下していたものが短絡結紮により上昇した
  • 【後頭蓋窩血管病変の全て】血行再建ほか 人工血管を用いた鎖骨下動脈再建術
    宝金 清博 The Mt. Fuji Workshop on CVD 17 80 -84 1999年07月 [査読無し][通常論文]
     
    Goretexを用いた鎖骨下動脈再建手術8例を報告した.術後十分なwarfarinzationを行えば長期開存が得られ,手術手技も容易で,優れた方法であった.総頸動脈遮断は安全と考えられるが,fail-safeな手術のために術中モニタリング下に試験遮断を行い,少しでも危険な要素があれば外頸動脈との吻合も考慮すべきである
  • T Yoshimoto, K Houkin, T Ishikawa, H Abe SURGICAL NEUROLOGY 52 (1) 68 -71 1999年07月 [査読無し][通常論文]
     
    BACKGROUND Opening the arachnoid membrane causes leakage of the cerebrospinal fluid (CSF). CSF spillage and collection in the subdural, epidural, and/or subcutaneous space are occasionally seen on a postoperative CT scan. Closure of the arachnoid membrane is considered to be unnecessary because most of the fluid in the extracerebral space is temporary and disappears spontaneously. However, in some cases, this CSF collection results in serious complications such as brain compression, extracerebral hematoma, intractable meningitis, and skin problems. In this study for prevention of CSF leakage, the arachnoid membrane was tightly closed with 10-0 nylon after a small opening was made for cerebrovascular bypass surgery. METHODS The arachnoid membrane closure was performed in 18 patients (20 sides) after bypass surgery between 1994 and 1995. As a control, 51 patients (57 sides) had conventional bypass surgery without the arachnoid membrane closure. RESULTS The radiological findings revealed that only one (5%) of the patients had a small fluid collection after arachnoid membrane closure, which was significantly lower than the control (49%). CONCLUSION Arachnoid membrane closure is a simple technique and is effective for prevention of postoperative extracerebral CSF collection. (C) 1999 by Elsevier Science Inc.
  • S Ushikoshi, Y Kikuchi, K Houkin, K Miyasaka, H Abe NEUROLOGIA MEDICO-CHIRURGICA 39 (7) 524 -529 1999年07月 [査読無し][通常論文]
     
    An 81-year-old male presented with right oculomotor nerve paresis and left hemiparesis caused by a mass effect of a large superior cerebellar artery aneurysm. Endovascular treatment was performed using Guglielmi detachable coils. The patient subsequently suffered aggravation of the mass effect 3 weeks after the embolization. Bilateral vertebral artery occlusion was performed, which decreased the cerebral edema surrounding the aneurysm, but his neurological symptoms did not improve. Parent artery occlusion is recommended as the first choice of treatment for an unclippable large or giant aneurysm causing a mass effect on the brainstem.
  • 宝金 清博, 七戸 秀夫, 黒田 敏, 松居 喜朗, 牛越 聡 治療学 33 (5) 582 -584 1999年05月 [査読無し][通常論文]
  • K Houkin, H Kamiyama, S Kuroda, T Ishikawa, A Takahashi, H Abe JOURNAL OF NEUROSURGERY 90 (4) 786 -790 1999年04月 [査読無し][通常論文]
     
    Reconstruction of the carotid artery by using a radial artery graft is a useful option that can produce reliable long-term patency for the surgical treatment of giant and/or large aneurysms of the cavernous and paraclinoid internal carotid artery (ICA). During the past 10 years, 43 patients with intracavernous and paraclinoid giant aneurysms of the ICA have been treated by reconstruction of the ICA with radial artery grafts after ligation of the cervical ICA. The long-term patency of the grafted radial artery was evaluated over more than a 5-year period (mean 7.2 years) in 20 of these patients by using magnetic resonance angiography or conventional angiography. There was no late occlusion of the graft in any of these cases. Stenotic graft changes were observed in two cases.
  • 黒田 敏, 宝金 清博, 藤本 真, 布村 充, 阿部 弘 脳卒中 21 (1) 102 -102 1999年03月 [査読無し][通常論文]
  • 能條 建, 田村 守, 佐藤 知絵, 根本 正史, 宝金 清博, 黒田 敏, 阿部 弘 脳卒中 21 (1) 122 -122 1999年03月 [査読無し][通常論文]
  • 難波 理奈, 黒田 敏, 宝金 清博, 柏葉 武, 斎藤 久寿, 野村 三起夫, 阿部 弘 脳卒中 21 (1) 143 -143 1999年03月 [査読無し][通常論文]
  • 黒田 敏, 石川 達哉, 宝金 清博, 上山 博康, 中川 翼, 阿部 弘 脳卒中 21 (1) 108 -108 1999年03月 [査読無し][通常論文]
  • 宝金 清博, 中川 翼, 上山 博康, 黒田 敏, 石川 達哉, 高橋 明弘, 阿部 弘 Neurological Surgery 27 (3) 211 -222 1999年03月 [査読無し][通常論文]
  • M Nemoto, Y Nomura, C Sato, M Tamura, K Houkin, Koyanagi, I, H Abe JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM 19 (3) 246 -259 1999年03月 [査読無し][通常論文]
     
    The origins of reflected light changes associated with neuronal activity (optical signals) were investigated in rat somatosensory cortex with optical imaging, microspectrophotometry, and laser-Doppler flowmetry, and dynamic changes in local hemoglobin concentration and oxygenation were: focused on. Functional activation was carried out by 2-second, 5-Hz electrical stimulation of the hind limb under chloralose anesthesia. These measurements were performed at the contralateral parietal cortex through a thinned skull. Regional cortical blood flow (rCBF) started to rise 1.5 seconds after the stimulus onset, peaked at 3.5 seconds (26.7% +/- 9.7% increase over baseline), and returned to near baseline by 10 seconds. Optical signal responses at 577, 586, and 805 nm showed a monophasic increase in absorbance coincident with the increase in rCBF; however, the signal responses at 605 and 760 nm were biphasic (an early increase and late decrease in absorbance) and micro-anatomically heterogeneous. The spectral changes of absorbance indicated that the concentrations of both total hemoglobin and oxyhemoglobin increased together with rCBF; deoxyhemoglobin, increased slightly but distinctly (P = 0.016 at 1.0 seconds, P = 0.00038 at 1.5 seconds) just before rCBF increases, then decreased. The authors conclude that activity-related optical signals are greatly associated with a moment-to-moment adjustment of rCBF and metabolism to neuronal activity.
  • K Houkin NEUROLOGICAL SURGERY 27 (3) 211 -222 1999年03月 [査読無し][通常論文]
  • ISHIKAWA Tatsuya, HOUKIN Kiyohiro, AKINO Minoru, MATSUZAWA Hitoshi, SAITOH Hisatoshi, ABE Hiroshi Journal of stroke and cerebrovascular diseases : official publication of the National Stroke Association 8 (2) 76 -83 1999年03月01日 [査読無し][通常論文]
  • ISHIKAWA Tatsuya, HOUKIN Kiyohiro, SAWAMURA Yutaka, AOYAMA Takeshi, ABE Hiroshi Journal of stroke and cerebrovascular diseases : official publication of the National Stroke Association 8 (2) 94 -98 1999年03月01日 [査読無し][通常論文]
  • 拡散強調画像(DWI)による急性期脳虚血の診断
    黒田 敏, 藤本 真, 数又 研, 布村 充, 宝金 清博, 阿部 弘 日本神経救急研究会雑誌 12 85 -88 1999年02月 [査読無し][通常論文]
     
    DWIは従来のmodalityと比較して,発症直後より虚血脳の局在を診断するには極めて有用であり,虚血性心疾患における心電図(ECG)と同様,重要な役割を果たしていくと考えられた
  • K. Houkin, S. Kuroda, A. Takahashi, S. Takikawa, T. Ishikawa, T. Yoshimoto, K. Itamato Acta Neurochirurgica 141 (12) 1255 -1263 1999年 [査読無し][通常論文]
     
    The causes and management of intra-operative premature rupture are analysed and discussed. During the past 6 years, the authors, performed 398 consecutive direct surgical interventions for ruptured cerebral aneurysms. Intraoperative premature rupture is defined as a rupture which occurs before the securing of the parent arteries or the neck of the aneurysm and is out of control, at least temporarily. The causes and management were retrospectively analysed by reviewing video tape recordings. Intra-operative premature ruptures which met the above definition occurred in 24 cases (6.0%). The causes were as follows: 1.) dural opening and arachnoid opening (8.3%), 2.) haematoma removal (12.5%), 3.) brain retraction (16.7%). 4.) aneurysm dissection (62.5%). A double suction technique was used to control bleeding and haemostasis with a small piece of cotton or a temporary clip, performed in 20 cases (83.3%). However, in cases with premature rupture immediately after the dural or arachnoid opening, the extension of the haematoma into the subarachnoid space resulted in severe brain swelling and partial resection of the brain had to be done to secure temporary clipping. The double suction technique and primary haemostasis using a small piece of cotton or temporary clip resulted in good outcome even in cases with premature rupture. However, very early premature rupture also occurred although its incidence was extremely rare. The removal of part of the brain can secure the working space but the outcome was poor.
  • Cerebral Revascularization for Moyamoya Disease.
    Operative Techniques in Neurosurgery 2 116 -122 1999年 [査読無し][通常論文]
  • Cerebrovascular Disease: Ischemia, b. Extracranial Lesion
    脳神経外科レビュー(7) 1999年 [査読無し][通常論文]
  • Cerebrovascular Disease: Ischemia, a. Intracranial Lesion
    脳神経外科レビュー(7) 1999年 [査読無し][通常論文]
  • 興梠 征典, 宝金 清博 脳神経外科ジャーナル 8 (4) 246 -246 1999年 [査読無し][通常論文]
  • 宝金 清博 脳神経外科ジャーナル 8 (4) 246 -246 1999年 [査読無し][通常論文]
  • 神経活動に伴う内因性光シグナルとFlow Oscillation由来の光シグナル Rat大脳皮質での観察
    根本 正史, 田村 守, 宝金 清博, 柏葉 武, 阿部 弘 脳循環代謝 10 (3) 298 -299 1998年12月 [査読無し][通常論文]
  • 石川 達哉, 宝金 清博, 田中 徳彦, 阿部 弘 臨床小児医学 46 (6) 285 -286 1998年12月 [査読無し][通常論文]
  • 宝金 清博 脳卒中の外科 = Surgery for cerebral stroke 26 (6) 457 -459 1998年11月30日 [査読無し][通常論文]
  • 石川 達哉, 黒田 敏, 宝金 清博, 上山 博康, 阿部 弘 Neurological Surgery 26 (9) 823 -829 1998年09月 [査読無し][通常論文]
     
    hemodynamic compromiseを有する49症例(最初に分析した28症例(Group 1),その後に経験した21症例(Group 2))のEC-IC bypassの術前後の脳循環動態をresting mCBFとacetazolamide反応性にて評価し,脳虚血発作の再発との関連を調べた. 1)EC-IC bypassにより,resting mCBFには術前後の変化が無かったが,障害されていたacetazolamide反応性は改善した. 2)術前及び術後にresting mCBFの有意に低下しているgroupでは,脳虚血発作の再発率が有意に高かった. 3)1),2)の結論はGroup 1,2とも同様に認められ,総合して脳虚血発作の再発率は年間4.4%であった
  • T Ishikawa, S Kuroda, K Hokin, H Kamiyama, H Abe NEUROLOGICAL SURGERY 26 (9) 823 -829 1998年09月 [査読無し][通常論文]
     
    The effectiveness of extracranial-intracranial arterial bypass (EC-IC bypass) surgery for patients with hemodynamic compromise still remains controversial. In the present study, we Evaluated the correlation between the pre- and post-surgical cerebral hemodynamics and long-term prognosis. 28 patients and a subsequent 21 patients (41 men, eight women: mean age 59.9 [S.D. 8.6] years) with reduced cerebrovascular reserve due to steno-occlusive disease of the cerebral major arteries formed the study groups 1 and 2, respectively. Measurement of the mean hemispheric cerebral blood flow (mCBF) and the cerebral vasoreactivity (%mCVR) with an intravenous acetazolamide injection were performed by a Xe-133 inhalation method and SPECT. patients were treated with EC-IC bypass surgery and measurement of mCBF and %mCVR were made again about one month after surgery. The patients were observed for a long period (mean 44.3 months). During the follow-up period, 6 patients experienced recurrent ischemic strokes. The annual incidence of recurrent ischemic stroke was 4.4%. The patients with significantly reduced pre- and post-surgical resting mCBF of the affected hemisphere were at significantly higher risk of recurrent ischemic stroke than the patients with normal mCBF (p<0.01). The %mCVR of the affected hemisphere rose after surgery.
  • Kiyohiro Houkin, Naoki Nakayama, Kyousuke Kamada, Takeshi Noujou, Hiroshi Abe, Takeshi Kashiwaba Journal of Stroke and Cerebrovascular Diseases 7 (5) 315 -322 1998年09月 [査読無し][通常論文]
     
    A newly developed free radical scavenger, 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186), holds promise for clinical application. We clinically evaluated the effect of MCI-186 on cerebral infarction by using magnetic resonance imaging (MRI) and proton MR spectroscopy (MRS). Six patients with large supratentorial infarction were evaluated with sequential MRI and proton MRS. These patients were also administered MCI-186 for 14 days after ischemic insult (MCI-186 group). The findings were compared with those for patients who had supratentorial infarctions equivalent in size to those in the MCI-186 group but who had received only conventional therapy. The course of change of the size of infarction was evaluated by MRI, and the metabolic changes following cerebral infarction were evaluated by proton MRS. As a result, there was no significant difference between the initial size of infarction in the conventionally treated group and that in the MCI-186 treated groups, nor did the groups show significant difference in the sequential changes depicted by MRI in the area of infarction, midline shift, or amount of edema. However, on MRS, the N-acetyl aspartate signal was significantly higher in the MCI-186 group than in the conventionally treated patients. In conclusion, MCI-186 has an effect of preservation of N-acetyl-aspartate, which is thought to be a neuronal marker, in cerebral infarction. © 1998 National Stroke Association.
  • S Ushikoshi, Y Kikuchi, K Houkin, H Saito, H Abe NEUROLOGIA MEDICO-CHIRURGICA 38 (8) 478 -484 1998年08月 [査読無し][通常論文]
     
    Four patients with multiple intracranial dural arteriovenous fistulas (DAVFs) at separate sites were treated by endovascular techniques (transarterial and/or transvenous embolization), surgery (excision or isolation), radiotherapy, or combinations, according to the pathophysiological condition. All lesions in two patients were obliterated completely without neurological deficit. There were residual fistulas after the treatment in two patients, but these were low-grade lesions without retrograde cortical venous drainage, and marked clinical improvement was obtained. Planning of treatment strategies for multiple DAVFs requires careful analysis of the venous drainage from the affected sinuses and cerebral hemodynamics.
  • T Ishikawa, N Tanaka, K Houkin, S Kuroda, H Abe, K Mitsumori CHILDS NERVOUS SYSTEM 14 (8) 366 -371 1998年08月 [査読無し][通常論文]
     
    Thirteen pediatric patients (ages 4-13 years) who underwent surgical treatment were examined regarding their rCBF in the preoperative periods. The postoperative rCBF was measured 39 times in these 13 patients. Thirteen healthy normal subjects (ages from 6 to 21 years) were also examined. The rCBF in the operculum and in the frontal, parietal, and occipital lobes was measured with Xe-133 inhalation method and single photon emission computed tomography. In the parietal and occipital lobes, the preoperative rCBF had a negative and significant correlation with their ages, but not in the operculum or frontal lobe, However, subsequent to the surgical treatment, the I CBF increased significantly in the patients 5 years old or less, and then post-operative rCBF values had significant negative correlations with age in each region.
  • アニラセタム(ドラガノン)投与により臨床症状の改善とMRS上の改善がみられた1例
    宝金 清博, 石川 達哉 老化と疾患 11 (8) 1182 -1186 1998年07月 [査読無し][通常論文]
  • T Yoshimoto, K Houkin, Y Nakagawa, H Kamiyama, H Abe JOURNAL OF CLINICAL NEUROSCIENCE 5 (3) 357 -360 1998年07月 [査読無し][通常論文]
     
    For surgical therapy of stenosis and occlusion of the subclavian artery, several types of extrathoracic vascular reconstructions have recently been performed, mainly in the supraclavicular fossa. Carotid-subclavian bypass is becoming a more acceptable surgical procedure than axilloaxillary bypass. We encountered three patients with these lesions who underwent common carotid-subclavian bypass with autogenous saphenous vein grafts, Their symptoms were completely relieved and pulsation of the radial artery recovered sufficiently. Restoration of inflow from the carotid artery was able to provide adequate reconstruction of the subclavian lesions. This bypass is less invasive and is efficient for such lesions in the supraclavicular position.
  • 瀧川 修吾, 宝金 清博, 斎藤 久寿, 阿部 弘 脳卒中の外科 = Surgery for cerebral stroke 27 (0) 72 -72 1998年06月24日 [査読無し][通常論文]
  • 石川 達哉, 黒田 敏, 宝金 清博, 阿部 弘 脳卒中の外科 = Surgery for cerebral stroke 27 (0) 318 -318 1998年06月24日 [査読無し][通常論文]
  • 宝金 清博, 石川 達哉, 黒田 敏, 阿部 弘 脳卒中の外科 = Surgery for cerebral stroke 27 (0) 320 -320 1998年06月24日 [査読無し][通常論文]
  • 宝金 清博, 石川 達哉, 阿部 弘 脳卒中の外科 = Surgery for cerebral stroke 27 (0) 329 -329 1998年06月24日 [査読無し][通常論文]
  • 小林 徹, 宝金 清博, 伊藤 文生, 小浜 好彦 脳卒中の外科 = Surgery for cerebral stroke 27 (0) 331 -331 1998年06月24日 [査読無し][通常論文]
  • 成人モヤモヤ病虚血発症例の検討
    山内 亨, 石川 達哉, 宝金 清博, 阿部 弘, 柏葉 武, 斉藤 久寿 脳循環代謝 10 (1) 122 -123 1998年06月 [査読無し][通常論文]
  • S Kuroda, S Ushikoshi, K Houkin, H Saito, Y Kikuchi, H Abe SURGICAL NEUROLOGY 49 (4) 406 -411 1998年04月 [査読無し][通常論文]
     
    BACKGROUND It is well known that carotid endarterectomy and extracranial-intracranial arterial bypass sometimes cause postoperative hyperperfusion, and vasoparalysis attributable to long-standing ischemia has been suggested as the cause. It is also well known that dural arteriovenous fistula (AVF) sometimes causes cerebral ischemia attributable to venous hypertension. However, there are few reports regarding the postoperative changes of regional cerebral blood flow (rCBF). METHODS We report a case of dural AVF of the left transverse/sigmoid sinuses, occurring in a 64-year-old man. Intraoperative transvenous embolization combined with transarterial embolization was performed, and the rCBF was measured pre-and postoperatively using Tc-99m-hexamethyl-propylene amine oxime and single-photon emission computed tomography (SPECT). RESULTS Preoperative SPECT disclosed a marked rCBF reduction in the left temporal, parietal, and occipital lobes. Complete obliteration of the AVF was attained after the intraoperative transvenous embolization, without any neurological deterioration. However, postoperative SPECT temporary hyperperfusion in these regions. CONCLUSIONS Sudden resolution of venous ischemia can lead to postoperative hyperperfusion, and pre-and post-treatment rCBF studies are important to prevent complications related to hyperperfusion. (C) 1998 by Elsevier Science Inc.
  • 中村 俊孝, 宝金 清博, 阿部 弘, 瀧川 修吾, 斉藤 久寿 CI研究 20 (1) 37 -43 1998年03月 [査読無し][通常論文]
     
    123I-IMP SPECTにより,慢性期脳虚血症例11例の血行再建術前後のCBFを検討した.血行再建術の適応判定には,CBFの詳細な検討が必要であると思われた
  • K Asaoka, K Houkin, S Fujimoto, T Ishikawa, H Abe NEUROSURGERY 42 (1) 157 -160 1998年01月 [査読無し][通常論文]
     
    OBJECTIVE AND IMPORTANCE: Aortitis syndrome is a systemic vasculitis of unknown etiology, affecting mainly the major branches of the aorta and leading to stenosis or occlusion. Intracranial aneurysms are rarely associated with this syndrome. Only 15 cases have been previously reported. CLINICAL PRESENTATION: A 54-year-old woman with a 10-year history of aortitis syndrome demonstrated nonruptured cerebral aneurysms in the left internal carotid and anterior communicating arteries, with decreased flow in the right internal carotid artery and ipsilateral Al segment. INTERVENTION: The patient underwent clipping of the aneurysms through a left pterional craniotomy and transsylvian approach. CONCLUSION: In our review of 16 reported cases, including the present case (13 with ruptured aneurysms and 3 with nonruptured aneurysms), there were 25 saccular aneurysms, and we noted the following clinical characteristics: 1) patient age was 26 to 64 years, with an average of 50.2 years; 2) aneurysms arose predominantly along the course of collateral flow, especially in the vertebrobasilar system (13 of 25 aneurysms, 52.0%); 3) there was a high incidence of multiplicity (7 of 16 cases, 43.8%). These characteristics suggest that increased hemodynamic stress, produced by stenosis or occlusion of the major branches of the aorta and systemic hypertension, plays an important role in the development of cerebral aneurysms in patients with this syndrome.
  • Neurologia Medico-chirurgica 28 1163 -1169 1998年 [査読無し][通常論文]
  • 頭蓋外血管閉塞病変の検索に有用なMR Aortography
    Medical Tribune 1998年 [査読無し][通常論文]
  • 脳幹・基底核〔機能・解剖・手術〕.
    メディカルビュー社 1998年 [査読無し][通常論文]
  • 3次元CT angiography
    脳血管障害の診断 1998年 [査読無し][通常論文]
  • 脳卒中学
    メディカルビュー社 1998年 [査読無し][通常論文]
  • Katsuyuki Asaoka, Kiyohiro Houkin, Shin Fujimoto, Tatsuya Ishikawa, Hiroshi Abe Neurosurgery 42 (1) 157 -161 1998年01月 [査読無し][通常論文]
     
    OBJECTIVE AND IMPORTANCE: Aortitis syndrome is a systemic vasculitis of unknown etiology, affecting mainly the major branches of the aorta and leading to stenosis or occlusion. Intracranial aneurysms are rarely associated with this syndrome. Only 15 cases have been previously reported. CLINICAL PRESENTATION: A 54-year-old woman with a 10-year history of aortitis syndrome demonstrated nonruptured cerebral aneurysms in the left internal carotid and anterior communicating arteries, with decreased flow in the right internal carotid artery and ipsilateral A1 segment. INTERVENTION: The patient underwent clipping of the aneurysms through a left pterional craniotomy and transsylvian approach. CONCLUSION: In our review of 16 reported cases, including the present case (13 with ruptured aneurysms and 3 with nonruptured aneurysms), there were 25 saccular aneurysms, and we noted the following clinical characteristics: 1) patient age was 26 to 64 years, with an average of 50.2 years 2) aneurysms arose predominantly along the course of collateral flow, especially in the vertebrobasilar system (13 of 25 aneurysms, 52.0%) 3) there was a high incidence of multiplicity (7 of 16 cases, 43.8%). These characteristics suggest that increased hemodynamic stress, produced by stenosis or occlusion of the major branches of the aorta and systemic hypertension, plays an important role in the development of cerebral aneurysms in patients with this syndrome.
  • Cooperation in Neurosurgery Between Japan and Malaysia-Proposal of New Tele-Communication System-.
    Fourth JSPS ASEAN-Japan Seminar The past and future of the Malaysia-Japan JSPS scientific cooperation program in medical science 1998年 [査読無し][通常論文]
  • 宝金 清博, 石川 達哉, 高橋 明弘 脳卒中の外科 26 (1) 38 -44 1998年01月 [査読無し][通常論文]
     
    もやもや病は原因の不明な疾患であるが現在,家族発症例を手掛かりとして遺伝子レベルでの原因究明が進んでいる.又,今回の結果に示した様に適切な診断に基づいた複合血行再建術を受けることにより,一過性脳虚血症状等の臨床症状の改善がみられる.その結果,患者は健常の小児と全く変わることのないQOLが期待できる.一方,治療時期を見逃すとその予後は必ずしも良好ではないことが明らかとなった.したがって今後の課題としては以下が挙げられた.1)早期発見の為のsystemの確立(家族発症例のscreening,軽症例の掘り起こし),2)MRAによる早期発見の普及,3)初期治療の重要性,4)治療後のQOLの向上(就学,就職の問題,美容上の問題)
  • 喜多村 圭, 鈴木 恵士郎, 白土 博樹, 澤村 豊, 宝金 清博, 加藤 功, 井須 豊彦, 阿部 弘, 宮坂 和夫 脳神経外科ジャーナル 7 (2) 102 -108 1998年 [査読無し][通常論文]
     
    LINAC脳定位放射線照射は飛躍的進歩の渦中にあり, 一方では用語の統一性が失われてきているため改めて整理する.聴神経鞘種に対する本治療の治療成績, 機能温存率, 合併症発生率は優れた外科的治療と同等と考えられる.脳血管奇形(AVM), 特に, small AVMに対しては1回照射の有効性が確立されたが, large AVMに対しては1回照射では閉塞率に限界がみえてきており, 少分割照射の試みもなされている.転移性脳腫瘍は頻繁に治療される疾患であり, その有効性も評価されているが, 外科的切除・全脳照射との兼ね合いが問題となっており, 今後のprospective randomized studyの結果が待たれる.
  • 吉本 哲之, SAWAMURA Yutaka, HOUKIN Kiyohiro, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 37 (12) 886 -890 1997年12月15日 [査読無し][通常論文]
     
    開頭術後に硬膜縫合部より出現する髄液漏れは、髄液漏の形成、髄膜炎や開頭部の感染等の合併症を発生させる危険性を有している。そのため硬膜縫合後の髄液漏出の防止を目的としてフィブリングルーの使用が増加しているが、その効果は明らかではない。今回、通常の前頭側頭開頭による未破裂動脈瘤の治療を受けた183人の患者を基にその効果を検討した。使用患者は138人であり、非使用患者は45人であった。術後、CT上、硬膜外に低吸収域を呈したものは55人認められたが、すべて一過性であった。そのうちフィブリングルーの使用が36人(26%)、非使用が19人(42%)であった。これよりフィブリングルーは髄液漏の頻度を有意に減少させ得ることが示された。
  • T Ishikawa, K Houkin, T Yoshimoto, H Abe SURGICAL NEUROLOGY 48 (6) 620 -626 1997年12月 [査読無し][通常論文]
     
    BACKGROUND Cerebral vasculopathy associated with the appearance of netlike vessels can develop following irradiation therapy for parasellar brain tumors, especially in children, However, little is known regarding the clinical course of this disease or the appropriate therapy for it. CASE REPORTS We experienced two surgically-treated patients with radiation-induced vasculopathy and reviewed the previously reported cases. Both of our patients were treated with encephalo-duro-arterio-myo-synangiosis combined with superficial temporal artery to middle cerebral artery (STA-MCA) bypass. Their ischemic symptoms improved following the surgery, associated with a good angiographic neovascularization from the STA-MCA bypass, as well as dural and muscular arteries, CONCLUSION Our findings and the review of the previous reports suggested that surgical therapy may be beneficial for the patients with radiation-induced vasculopathy with the appearance of netlike vessels. (C) 1997 by Elsevier Science Inc.
  • 宝金 清博, 阿部 弘 脈管学 37 (10) 781 -787 1997年10月25日 [査読無し][通常論文]
  • 中村 俊孝, HOUKIN Kiyohiro, SAITOH Hisatoshi, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 37 (10) 747 -751 1997年10月15日 [査読無し][通常論文]
     
    副中大脳動脈に合併した未破裂前交通動脈瘤の一例を報告する。症例は63才女性で慢性的な後頭部痛があり、精査の結果、未破裂前交通動脈瘤が認められた。動脈瘤は、重複前交通動脈瘤部に認められたが、この部より右の副中大脳動脈が認められた。右のHeubner動脈はなかった。こうした血管奇形の診断においては、3次元CT血管造影法が有用であった。手術は通常の前側頭開頭で行われ、術後経過良好で退院した。副中大脳動脈に合併した通動脈瘤は22例の報告があった。多くは、前交通動脈あるいは前大脳動脈水平部に存在した。こうした副中大脳動脈は、Heubner動脈のvariationである可能性が高いと思われた。
  • 脳動脈瘤破裂による急性期くも膜下出血のMRI,MRA診断
    青樹 毅, 金子 貞男, 七戸 秀夫, 宝金 清博, 阿部 弘, 三森 研自 日本脳神経外科学会総会抄録集 56回 28 -28 1997年10月 [査読無し][通常論文]
  • 術後評価としての3D-CT angiographyの有用性 頸動脈内膜剥離術及び脳動脈瘤クリッピング術における検討
    中村 俊孝, 瀧川 修吾, 宝金 清博, 石川 達哉, 斎藤 久寿, 阿部 弘 日本脳神経外科学会総会抄録集 56回 120 -120 1997年10月 [査読無し][通常論文]
  • 血栓内膜剥離術(CEA)中の一時血流遮断は安全か? TCD及びNIRSによるモニタリング
    黒田 敏, 中村 俊孝, 宝金 清博, 石川 達哉, 高橋 明弘, 阿部 弘 日本脳神経外科学会総会抄録集 56回 170 -170 1997年10月 [査読無し][通常論文]
  • 相互交流方式による短期留学の効率化 マレーシア-日本学術交流
    Zurin Adnan, 宝金 清博, 阿部 弘, 福原 俊一 日本脳神経外科学会総会抄録集 56回 224 -224 1997年10月 [査読無し][通常論文]
  • 神経活動に伴う内因性光Signal/Rat大脳皮質での観察
    根本 正史, 田村 守, 宝金 清博, 阿部 弘 日本脳神経外科学会総会抄録集 56回 95 -95 1997年10月 [査読無し][通常論文]
  • 動脈瘤術中破裂は何故起こる? 原因分析とその対策
    宝金 清博, 石川 達哉, 阿部 弘 日本脳神経外科学会総会抄録集 56回 246 -246 1997年10月 [査読無し][通常論文]
  • EC-IC bypass術後脳虚血再発例の脳血流動態の検討
    瀧川 修吾, 宝金 清博, 石川 達哉, 斎藤 久寿, 阿部 弘 日本脳神経外科学会総会抄録集 56回 267 -267 1997年10月 [査読無し][通常論文]
  • MR Aortographyはどこまで有用か? 頭蓋外閉塞性血管病変のScreening Testとして
    石川 達哉, 宝金 清博, 滝川 修吾, 秋野 実, 松沢 等, 斉藤 久寿, 阿部 弘 日本脳神経外科学会総会抄録集 56回 93 -93 1997年10月 [査読無し][通常論文]
  • 1H-CSIを用いた脳梗塞の経時的観察及び,梗塞範囲の推定についての検討
    浅野 剛, 宝金 清博, 松沢 等, 斉藤 久寿, 阿部 弘 日本脳神経外科学会総会抄録集 56回 97 -97 1997年10月 [査読無し][通常論文]
  • 血管内外科治療のpitfallに対する外科治療
    高橋 明弘, 宝金 清博, 阿部 弘, 菊地 陽一, 滝川 修吾, 斎藤 久寿, 柏葉 武 日本脳神経外科学会総会抄録集 56回 125 -125 1997年10月 [査読無し][通常論文]
  • もやもや病主幹動脈の免疫病理学的検討
    吉本 哲之, 宝金 清博, 阿部 弘, 長嶋 和郎, 竹田 誠, 井須 豊彦, 三森 研自 日本脳神経外科学会総会抄録集 56回 169 -169 1997年10月 [査読無し][通常論文]
  • 宝金 清博, 阿部 弘 脈管学 37 (10) 781 -787 1997年10月 [査読無し][通常論文]
     
    15歳以下の小児例34例,16歳以上の成人例33例の経験.手術は浅側頭動脈-中大脳動脈吻合術と脳-硬膜-血管-筋肉接着術を併用した.小児例では全例で虚血症状の消失ないし軽快が認められた.成人でも虚血例では血行再建により虚血症状の改善が認められた.出血例では外科治療後にも再出血がみられた
  • Toshitaka Nakamura, Kiyohiro Houkin, Hisatoshi Saitoh, Hiroshi Abe Neurologia Medico-Chirurgica 37 (10) 747 -751 1997年10月 [査読無し][通常論文]
     
    A 63-year-old female presented with an anterior cerebral artery aneurysm associated with accessory middle cerebral artery. The aneurysm was located on one of the duplicate anterior communicating arteries, and the accessory middle cerebral artery originated from the anterior cerebral artery adjacent to this aneurysm. The recurrent artery of Heubner was not recognized. The accessory middle cerebral artery may have been involved in the genesis of the aneurysm by changing the hemodynamics in the anterior cerebral arteries.
  • K Houkin, T Ishikawa, T Yoshimoto, H Abe CLINICAL NEUROLOGY AND NEUROSURGERY 99 S142 -S145 1997年10月 [査読無し][通常論文]
     
    We have performed surgical treatment for Moyamoya disease using the superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis and encephalo-duro-arterio-myo-synangiosis (EDAMS). In this paper, the surgical technique of combined revascularization for Moyamoya disease as well as peri-operative complications are discussed. Craniotomy and dural opening were extensively carried out to expose the brain surface as widely as possible. Dissection of the STA, which is the most powerful resource of direct revascularization, should be carefully carried out using a surgical microscope. The temporal muscle and middle meningeal artery, which have the most potential as sources of indirect revascularization, must be preserved. STA-MCA anastomosis to the frontal branch of the middle cerebral artery is indispensable for improving cerebral circulation of the frontal lobe. A small arachnoid membrane opening and water-tight closure are also important to avoid pest-operative subdural and subcutaneous fluid collection. Ischemic events disappeared immediately after surgery in most cases. However, in several cases, transient ischemic attacks recurred for several months after the surgery. Chronic subdural hematoma was seen in two cases. (C) 1997 Elsevier Science B.V.
  • T Yamauchi, K Houkin, M Tada, H Abe CLINICAL NEUROLOGY AND NEUROSURGERY 99 S162 -S167 1997年10月 [査読無し][通常論文]
     
    There is extensive evidence that Moyamoya disease has a tendency for multifactorial inheritance, although the pathogenesis of Moyamoya disease is not clear. The authors report five cases showing familial occurrence of Moyamoya disease and analyse its clinical characteristics. In the past 15 years, we have encountered 68 cases of Moyamoya disease. Among these, 14 cases (10 females and four males, five family pedigrees, asymptomatic 1 case) of familial occurrence were observed. In this series, mother-to-child inheritance was observed in five cases, although there were no cases showing father-to-child inheritance. Ten patients were children with an initial onset of cerebral ischemia, at a mean age of 9.7 years. One mother was asymptomatic and two mothers had a past history of cerebral ischemia. Only one patient was a 37-year-old woman with clinical onset of intracerebral hemorrhage. There were no specific clinical characteristics in familial Moyamoya disease compared with those in sporadic Moyamoya disease. (C) 1997 Elsevier Science B.V.
  • T Yoshimoto, K Houkin, A Takahashi, H Abe CLINICAL NEUROLOGY AND NEUROSURGERY 99 S218 -S220 1997年10月 [査読無し][通常論文]
     
    We investigated the levels of angiogenic growth factors in cerebrospinal fluid (CSF) from patients with Moyamoya disease and from those with atherosclerotic occlusive disease to evaluate the relationship of these factors to the pathogenesis of Moyamoya disease. CSF from Moyamoya patients contained significantly higher concentrations of basic fibroblast growth factor (b-FGF) (P < 0.05). The b-FGF level was apparently elevated in patients with well developed neovascularization after indirect revascularization surgery (P < 0.01). The other angiogenic factors were not significantly elevated compared with those of the control group. (C) 1997 Elsevier Science B.V.
  • K Kamada, K Houkin, K Hida, H Abe JOURNAL OF CLINICAL NEUROSCIENCE 4 (4) 473 -478 1997年10月 [査読無し][通常論文]
     
    It was hypothesized that AVS 1,2-bis(nicotinamide)-propane, a free radical scavenger, could potentially be useful for the treatment of brain edema, Vasogenic brain edema was produced by freezing brain regions in 36 rats that had been treated with either AVS or saline, The efficacy of treatment was analyzed using proton magnetic resonance imaging (H-1 MRI) and proton magnetic resonance spectroscopy (H-1 MRS) of perchloric acid tissue extracts, T2-weighted MRI was obtained at 1 and 24 h after freezing, For H-1 MRS studies, rats were killed using liquid nitrogen and the extracted metabolites from the frozen hemispheres analyzed. H-1 MRI showed the edema lesion that indicated a region of a high signal intensity gradually extended and increased in intensity, Suppression of the edema development was observed in the AVS-treated group, Shortly after injury, the levels of acetate and lactate rapidly rose, Subsequently, the amino acid of alanine was also increased and N-acetyl aspartate and glutamine were markedly depressed, In AVS-treated rats, increases of lactate and alanine, and decreases of N-acetyl aspartate were statistically significant, We concluded that AVS is valuable for the treatment of vasogenic brain edema using these MR techniques. (C) Pearson Professional Ltd 1997.
  • 牛越 聡, 斎藤 久寿, 宝金 清博, 阿部 弘, 菊地 陽一 新潟医学会雑誌 111 (9) 584 -584 1997年09月 [査読無し][通常論文]
  • 中村 俊孝, 宝金 清博, 石川 達哉, 高橋 明弘, 阿部 弘 新潟医学会雑誌 111 (9) 586 -586 1997年09月 [査読無し][通常論文]
  • 浅野 剛, 斉藤 久寿, 宝金 清博, 松沢 等, 阿部 弘 新潟医学会雑誌 111 (9) 589 -589 1997年09月 [査読無し][通常論文]
  • 宝金 清博 新潟医学会雑誌 111 (9) 592 -592 1997年09月 [査読無し][通常論文]
  • T Yoshimoto, J Nishihira, M Tada, K Houkin, H Abe NEUROSURGERY 41 (3) 648 -653 1997年09月 [査読無し][通常論文]
     
    OBJECTIVE: Macrophage migration inhibitory factor (MIF) is a cytokine that has the potential to immobilize and activate monocytes/macrophages. To examine whether MIF may potentially be involved in the pathogenesis of reperfusion injury of the brain, we investigated the expression of MIF in a rat model of reperfusion. METHODS: A four-vessel occlusion procedure was performed for 30 minutes using male Wistar rats to obtain a moderate reperfusion in the forebrains. Semiquantitatively calibrated reverse-transcription polymerase chain reaction analysis was conducted to examine temporal profiles of messenger ribonucleic acid (mRNA) expression for MIF and macrophage chemoattractant protein 1. MIF protein expression was assessed with specific Western blot analysis, For anatomic mapping of MIF, an immunohistochemical study was performed. RESULTS: Reverse-transcription polymerase chain reaction demonstrated that the mRNA level of MIF increased depending on the duration of reperfusion (less than or equal to 24 h) subsequent to global ischemia, The macrophage chemoattractant protein I mRNA was also observed to increase after reperfusional stress, but ifs maximum expression was reached earlier (1 h after the stress) than was MIF mRNA, Increase of MIF protein was also shown by Western blot. MIF-positive staining was observed in the neuronal processes (neuropil) in the cortex and basal ganglia of a rat forebrain. CONCLUSION: This protein is up-regulated and may modulate immunological reaction in secondary brain damage after ischemia and reperfusion stress.
  • T Yoshimoto, K Houkin, S Kuroda, H Abe, T Kashiwaba SURGICAL NEUROLOGY 48 (2) 132 -138 1997年08月 [査読無し][通常論文]
     
    BACKGROUND Hyperperfusion syndrome after surgical revascularization is a rare complication and there has not been any systematic study on factors that induce hyperperfusion after surgery. In this paper, we retrospectively analyzed the factors related to this syndrome. PATIENTS AND METHODS We performed 46 cases of surgical revascularization including 33 cases of carotid endarterectomy (CEA) and 13 cases of superficial temporal artery-middle cerebral artery (STA-MCA) anastomosis during the past 5 years. Among these, we encountered three cases of hyperperfusion syndrome despite well-controlled blood pressure postoperatively. To evaluate factors related to the occurrence of hyperperfusion syndrome, we examined four parameters: (1) regional cerebral blood flow (rCBF), (2) the increase in the ratio of the postoperative rCBF compared to the preoperative rCBF (increase ratio), (3) cerebral perfusion reserve presented by the increase of rCBF after acetazolamide administration (Delta rCBF), and (4) the difference in mean blood pressure between the preoperative and postoperative state (Delta BP). RESULTS Preoperative rCBF was significantly lower in cases of hyperperfusion syndrome than the control cases (p < 0.01 Mann-Whitney U-test). Moreover Delta rCBF was evidently lower in the hyperperfusion cases than the control (p < 0.05 Fisher's exact method). However, there was no significant difference in the Delta BP between the hyperperfusion cases and the control cases. CONCLUSION In cases of marked low perfusion (low rCBF) with poor perfusion reserve (low Delta rCBF), hyperperfusion after surgical revascularization can occur even if blood pressure is adequately controlled. (C) 1997 by Elsevier Science Inc.
  • K Kamada, K Houkin, Y Iwasaki, H Abe, T Kashiwaba NEURORADIOLOGY 39 (8) 560 -565 1997年08月 [査読無し][通常論文]
     
    The details of brain metabolism in chronic cerebral infarcts have not been clarified. Using proton MR spectroscopy (H-1-MRS) at 1.5 T, we measured biochemical changes in 16 patients with large infarcts involving the motor cortex in the chronic phase (median 293.9 days) and related the findings to clinical data. Localised spectra were obtained using point-resolved spectroscopy, with an echo time of 270 ms. Regions of interest were placed on the frontal lobe. including the precentral gyrus and central sulcus, Motor function was assessed by the manual muscle power test at the time of the H-1-MRS study. Only three patients with severe paresis had no signal in the lesions and a lactate signal was obtained in 13 cases, N-acetyl aspartate (NAA) was observed in 3 cases with recanalisation of an occluded vessel. Motor function correlated strongly with the NAA/choline-containing compounds (Cho) ratio (P < 0.01) and lactate/Cho ratio (P < 0.01). We found various metabolic patterns, reflecting residual neurological function.
  • ZURIN Adnan Abd Rahman, HOUKIN Kiyohiro, ASANO Tsuyoshi, ISHIKAWA Tatsuya, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 37 (7) 542 -545 1997年07月15日 [査読無し][通常論文]
     
    脳虚血発作にて発症した頭蓋内血管のfibromuscular dysplasia(FMD)の小児例を報告する。症例は8才女児、頭痛と左片麻痺にて発症した。MRIにて右被殻及び前頭葉に梗塞巣を認め、脳血管撮影では右内頚動脈終末部から中大脳動脈に及ぶstring of beads signが明らかとなった。保存的治療が行われ、神経学的脱落症状はほぼ改善した。発作から2ヶ月後のMR Angiographyでは中大脳動脈の病変は同様であったが、内頚動脈の狭窄は改善していた。FMDは小児の脳虚血の原因疾患として鑑別の必要な疾患である。
  • 宝金 清博 Neurological Surgery 25 (7) 587 -597 1997年07月 [査読無し][通常論文]
  • AAR Zurin, S Ushikoshi, K Houkin, Y Kikuchi, H Abe, H Saitoh JOURNAL OF NEUROSURGERY 87 (1) 109 -112 1997年07月 [査読無し][通常論文]
     
    This 63-year-old man presented with a right temporoparietal cortical infarction. A dural arteriovenous fistula involving the right transverse sinus was diagnosed on cerebral angiography. Transvenous embolization using detachable coils was performed; however, postembolization angiograms demonstrated retrograde filling of a cortical draining vein that was not seen on initial angiography. The patient subsequently developed a cerebral abscess in the region of the previous cortical infarction 2 months after the embolization. The abscess was successfully created with drainage and antibiotic therapy. The authors report this case to illustrate an unusual complication associated with this procedure and the possible contribution of the cortical draining vein in the pathogenesis of the cerebral abscess.
  • K Houkin NEUROLOGICAL SURGERY 25 (7) 587 -597 1997年07月 [査読無し][通常論文]
  • T Ishikawa, K Houkin, H Kamiyama, H Abe STROKE 28 (6) 1170 -1173 1997年06月 [査読無し][通常論文]
     
    Background and Purpose We reviewed surgically treated patients with pediatric moyamoya disease and examined whether vasoreconstructive surgeries reduced the risk of recurrent ischemic attacks and changed overall outcomes in terms of the patients' performance and intellectual status. Methods Sixty-four hemispheric sides in 34 pediatric moyamoya disease patients who received surgical treatment were examined. We performed superficial temporal artery to middle cerebral artery (STA-MCA) bypass and encephalo-duro-arterio-myo-synangiosis (EDAMS) on 48 sides (combined group) and indirect bypass surgery such as EDAMS on 16 sides (indirect group). These 34 patients were observed postoperatively from 1 to 14 years (mean +/- SD, 6.6 +/- 3.8 years) and were examined for the incidence of recurrent ischemic attack. Of the 34 patients, 23 were followed up for > 5 postoperative years, and their overall outcomes in terms of their performance and intellectual status were determined. Results Perioperative ischemic events (less than or equal to 2 weeks after surgery) occurred in 5 surgeries (31%) of the indirect group and in 6 (13%) of the combined group (P = NS). The incidence of postoperative ischemic events (> 2 weeks after surgery) was significantly reduced in the combined group (10%) compared with the indirect group (56%; P < .01). Of the 23 patients observed > 5 years, 7 patients (30%) were mentally retarded and regarded as having a fair outcome. Conclusions Combined surgery (STA-MCA bypass with EDAMS) for pediatric moyamoya disease was effective in reducing the risk of postoperative ischemic attacks compared with indirect surgery. Surgical revascularization may be effective in preventing intellectual deterioration and improving overall outcome.
  • 瀧川 修吾, 宝金 清博, 井戸坂 弘之 脳卒中の外科 25 (3) 189 -194 1997年05月 [査読無し][通常論文]
     
    チタン脳動脈瘤クリップを用いてneck clippingを行った26例29動脈瘤について検討を行った.チタンクリップは従来のクリップに比べCTやMRへの影響が極めて少なく,術後の画像診断の点からは非常に有用である.しかしクリップの強度及び閉鎖圧はこれまでのものより劣っており,現時点では使用に際してその特性を充分理解した上で適応症例を慎重に選択する必要がある
  • 鎌田 恭輔, 宝金 清博, 阿部 弘, 澤村 豊, 柏葉 武 Neurologia medico-chirurgica = 神経外科 37 (3) 250 -256 1997年03月15日 [査読無し][通常論文]
     
    高線量放射線照射後の脳腫瘍患者11例で水素磁気共鳴スペクトロスコピー(1H MRS)を用いて，放射線壊死および再発脳腫瘍の鑑別診断を施行した．6例の再発脳腫瘍および4例の放射線壊死は組織学的に診断が確認され，1例は臨床経過と画像所見より放射線壊死と診断された．放射線壊死は再発脳腫瘍に比して，著名に上昇したlactate/creatine and phosphocreatine(Cr)比と低下したCholine-containing compounds/Cr比，または完全な無信号である2つの1H MRSパターンが認められた．N-acetyl aspartateはこれらの鑑別診断の指標とはならなかった．1H MRSによる脳および腫瘍組織代謝の検討は，放射線壊死と再発腫瘍との鑑別に有効であるものと考えられた．
  • 石川 達哉, 宝金 清博, 吉本 哲之, 上山 博康, 阿部 弘 新潟医学会雑誌 111 (3) 173 -173 1997年03月 [査読無し][通常論文]
  • 瀧川 修吾, 井戸坂 弘之, 中村 俊孝, 牛越 聡, 秋野 実, 斉藤 久寿, 宝金 清博, 阿部 弘 新潟医学会雑誌 111 (3) 174 -174 1997年03月 [査読無し][通常論文]
  • 青樹 毅, 三森 研自, 中川 端午, 桜木 貢, 北見 公一, 村田 順一, 阿部 弘, 宝金 清博 新潟医学会雑誌 111 (3) 191 -191 1997年03月 [査読無し][通常論文]
  • 中村 俊孝, 斉藤 久寿, 宝金 清博 映像情報 29 (4) 148 -153 1997年02月15日 [査読無し][通常論文]
  • 中村 俊孝, 斉藤 久寿, 宝金 清博 映像情報Medical 29 (4) 148 -153 1997年02月 [査読無し][通常論文]
  • T Yoshimoto, K Houkin, M Tada, H Abe ACTA NEUROPATHOLOGICA 93 (2) 154 -158 1997年02月 [査読無し][通常論文]
     
    Cellular damage secondary to reperfusion following ischemic insult has been hypothetically attributed to an inflammatory cascade concerted by cell-to-cell interactions. While the role of several cytokines and adhesion molecules in reperfusion injury of the brain has been explored to a certain extent, their regulatory and temporary profiles remain unclear. We have addressed the temporal features of the induction of mRNA for proinflammatory cytokines, adhesion molecules and chemokines at an acute phase subsequent to reperfusion in rat forebrain. Semiquantitatively calibrated reverse transcription-polymerase chain reaction analysis was employed to assess the relative expression of mRNA for intercellular adhesion molecule (ICAM)-1, interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-6, tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte-chemoattractant protein (MCP)-1, and macrophage migration inhibitory factor (MIF). The increase in mRNA from the basal levels after reperfusion followed one of two different patterns; an increase occurring as early as 1 h, or a slight increase continuing up to 24 h after reperfusion. The former pattern was seen for ICAM-1, IL-1 alpha, IL-1 beta, TNF-alpha, and MCP-1, and the latter for IL-6 and MIF. These results were consistent with the proinflammatory properties of the immediately induced cytokines, which may be involved in the initiation step of the inflammatory cascade, causing the secondary cellular responses and finally leading to further brain damage.
  • T Ishikawa, K Houkin, K Tokuda, S Kawaguchi, T Kashiwaba JOURNAL OF NEUROSURGERY 86 (2) 291 -293 1997年02月 [査読無し][通常論文]
     
    Dural arteriovenous malformations (AVMs) are considered to be acquired lesions that develop secondary to venous obstruction, which sometimes happens in head trauma. However, there has been a report of an anterior cranial fossa dural AVM that occurred independently of a history of head trauma, and there has been speculation that these malformations are congenital. The authors recount their experience with a patient who had an anterior cranial fossa dural AVM that was discovered incidentally. The lesion was fed by the bilateral anterior ethmoidal arteries and drained into the superior sagittal sinus via frontal cortical veins. The patient had a history of severe head trauma that had occurred 30 years earlier. This is the first case report in which a previous head trauma is strongly believed to be the cause of an anterior cranial fossa dural AVM. The authors postulate that anterior cranial fossa dural AVMs can develop secondary to a head trauma.
  • Tatsuya Ishikawa, Nishio Nakamura, Kiyohiro Houkin, Mikio Nomura Neurosurgery 40 (2) 403 -406 1997年02月 [査読無し][通常論文]
     
    OBJECTIVE AND IMPORTANCE: Although the incidence is low, a very small aneurysm with a thin wall and no neck arises at the superior wall of the supraclinoid portion of the internal carotid artery and is called a 'blister- like' aneurysm. However, the pathogenesis of such a vascular lesion remains uncertain. CLINICAL PRESENTATION: A 57-year-old man developed a fatal subarachnoid hemorrhage caused by the rupture of a blister-like aneurysm at the superior wall of the internal carotid artery. An autopsy was performed, and the lesion was pathologically examined. RESULTS: The internal elastic lamina and media had disappeared at the border between the eccentrically sclerotic and normal carotid wall. The gap in the internal elastica was covered with normal adventitia and fibrinous tissue. This portion was not composed of collagenous tissue as ordinarily seen in an aneurysmal wall. Neither infiltration of inflammatory cells nor dissection of the artery were observed. CONCLUSION: The blister-like aneurysm appeared to be a laceration of the carotid wall based on degeneration of the internal elastic lamina.
  • 第5回脳卒中外科国際ワークショップ（5th IWCVS） 報告
    脳神経外科 25 576 -577 1997年 [査読無し][通常論文]
  • 脳脊髄MRA
    中外医学社 1997年 [査読無し][通常論文]
  • もやもや病のMRA
    脳脊髄MRA 1997年 [査読無し][通常論文]
  • 脳動脈瘤のMRA
    脳脊髄MRA 1997年 [査読無し][通常論文]
  • 閉塞性脳血管病変のMRA
    脳脊髄MRA 1997年 [査読無し][通常論文]
  • 脳血管解剖とMRA
    脳脊髄MRA 1997年 [査読無し][通常論文]
  • Perfusion imageとdiffusion image
    脳脊髄MRA 1997年 [査読無し][通常論文]
  • 脳血管奇形のMRA
    脳脊髄MRA 1997年 [査読無し][通常論文]
  • 脳血管奇形のMRA
    脳脊髄MRA 1997年 [査読無し][通常論文]
  • K Houkin, H Kamiyama, A Takahashi, S Kuroda, H Abe CHILDS NERVOUS SYSTEM 13 (1) 24 -29 1997年01月 [査読無し][通常論文]
     
    We present surgical techniques for optimal revascularization in childhood moyamoya disease. During the past 10 years we performed revascularization surgery for childhood moyamoya disease using direct revascularization with superficial temporal artery to middle cerebral artery (STA-MCA) anastomosis and indirect revascularization of encephalo-duro-arterio-myo-synangiosis (EDAMS) 43 times. Points of this surgery are as follows: (1) craniotomy and dural opening for the most extensive possible exposure of the brain surface, (2) protective manipulation of the STA, temporal muscle and middle meningeal artery, (3) STA-MCA anastomosis to the frontal branch of the MCA to improve cerebral circulation of the frontal lobe using a small branch of the STA, and (4) a small opening in the arachnoid membrane and watertight closure.
  • M Nemoto, Y Nomura, M Tamura, C Sato, K Houkin, H Abe OXYGEN TRANSPORT TO TISSUE XIX 428 521 -531 1997年 [査読無し][通常論文]
  • ニューロサイエンスの新しい研究方法 磁気共鳴によるdiffusion解析
    松沢 等, 宝金 清博 Clinical Neuroscience 14 (12) 1346 -1347 1996年12月 [査読無し][通常論文]
  • 宝金 清博 INNERVISION 12 (1) 10 -13 1996年12月 [査読無し][通常論文]
  • 宝金 清博, YOSHIMOTO Tetsuyuki, KURODA Satoshi, ISHIKAWA Tatsuya, TAKAHASHI Akihiro, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 36 (11) 783 -788 1996年11月15日 [査読無し][通常論文]
     
    小児モヤモヤ病と成人モヤモヤ病の発症形式、血管撮影上の特徴を検討した。小児例と成人例で大きな差はなかったが、小児例では、第III期が多く、成人例では第IV期が多い傾向が見られた。出血例と虚血例での差はなく、また、男女差もなかった。血管撮影上の変化を見ても、小児例と成人例の間には、大きな動きは見られなかった。また、血管撮影の期と小児モヤモヤ病、成人モヤモヤ病の臨床症状の際立った特徴の間には、関連はなかった。これらの結果から、モヤモヤ病における血管撮影上の進行は、幼小児期にほぼ完成し、その後は停止すると思われた。モヤモヤ病の発症には、その他の、動脈硬化や脳血流量などの要因が関係していると考えられる。
  • K Houkin, H Abe, T Yoshimoto, A Takahashi JOURNAL OF NEUROSURGERY 85 (5) 772 -776 1996年11月 [査読無し][通常論文]
     
    Whether a diagnosis of moyamoya disease is justified in patients with typical angiographic evidence of moyamoya disease unilaterally and normal angiographic findings contralaterally remains controversial. In this study the authors analyzed longitudinal angiographic change, familial occurrence, and basic fibroblast growth factor (bFGF) concentration in the cerebrospinal fluid (CSF) of patients with ''unilateral'' moyamoya disease. Over a 10-year period, 10 cases of unilateral moyamoya disease were followed using conventional angiography or magnetic resonance angiography. Basic FGF in CSF, obtained from the subarachnoid space of the cerebral cortex during revascularization surgery, was measured in five cases. Among the 10 cases of unilateral moyamoya disease, only one pediatric case showed obvious signs of progression to typical bilateral disease. The other nine cases (including six adults and three children) remained stable throughout follow-up radiological examinations (magnetic resonance angiography) with a mean observation period of 3.5 years. There was no familial occurrence in these cases of unilateral moyamoya disease. Levels of bFGF, which are high in typical moyamoya disease, were low in these patients. The progression from unilateral moyamoya disease to the typical bilateral form of the disease appears to be infrequent. The low levels of bFGF in the CSF of these patients and the lack of familial occurrence strongly suggest the most cases of unilateral moyamoya disease, especially those found in adults, are distinct from typical bilateral moyamoya disease.
  • モヤモヤ病の病態と外科治療
    宝金 清博 脈管学 36 (9) 595 -595 1996年09月 [査読無し][通常論文]
  • K Houkin, H Kamiyama, H Abe, A Takahashi, S Kuroda STROKE 27 (8) 1342 -1346 1996年08月 [査読無し][通常論文]
     
    Background and Purpose It is well recognized that revascularization surgery using direct and/or indirect bypass provides effective surgical management for pediatric moyamoya disease. However, surgical treatment of the adult hemorrhagic type remains controversial. In this study, the effect of surgery for adult moyamoya disease was investigated. Methods We analyzed 35 patients with adult moyamoya disease (patient age, over 20 years), 24 patients with initial onset of intracerebral hemorrhage, and 11 patients with initial onset of cerebral ischemia who underwent both direct bypass surgery of the superficial temporal artery to the middle cerebral artery anastomosis and indirect revascularization of encephalo-duro-arterio-myo-synangiosis. Results Of 24 patients with hemorrhagic-type disease, 3 showed rebleeding; of 11 patients with the ischemic type, 2 showed intracerebral hemorrhage after surgery. Overall, 5 of 35 patients (14.3%) had hemorrhage after revascularization surgery (mean follow-up period, 6.4 years), Postoperative angiography revealed that direct anastomosis is effective whereas indirect revascularization is not always effective for adult moyamoya disease. Moyamoya vessels, which are supposed to be responsible for hemorrhage, decreased in 25% of patients. Conclusions Revascularization surgery cannot always pre vent rebleeding. However, a decrease in moyamoya vessels was induced by surgery, which may reduce the risk of hemorrhage more effectively than conservative treatment. In cases of adult moyamoya disease, direct bypass is particularly important, since the indirect revascularization is not as useful in adult cases as in pediatric cases.
  • K Kazumata, S Kuroda, K Houkin, H Abe, T Kiyohara, H Kobayashi, A Okuno CHILDS NERVOUS SYSTEM 12 (6) 339 -342 1996年06月 [査読無し][通常論文]
     
    An 8-year-old girl suffering from precocious puberty and pustular psoriasis with moyamoya disease is described. The possibilities of a causal relationship between precocious puberty and moyamoya disease are discussed. The relevance of the patient's earlier upper respiratory tract infections to the pustular psoriasis and moyamoya disease is also considered.
  • S Kuroda, K Houkin, H Abe, Y Hoshi, M Tamura SURGICAL NEUROLOGY 45 (5) 450 -458 1996年05月 [査読無し][通常論文]
     
    BACKGROUND Recent studies have indicated that near-infrared spectroscopy (NIRS) could continuously and noninvasively observe the changes in cerebral oxygenation state during hypoxia and ischemia, using their optical properties. Its validity and usefulness during cerebrovascular surgery, however, still remain to be clarified. METHODS Using NIPS, we continuously monitored the changes in the concentration of oxyhemoglobin, deoxyhemoglobin, and total hemoglobin ([oxy-Hb], [deoxy-Hb], and [total Hb], respectively) and redox state of cytochrome oxidase (cyt ox) during carotid endarterectomy for 22 patients, and we compared the NIPS responses with those of intraoperative somatosensory evoked potentials (SEP) and regional cerebral blood flow (rCBF). RESULTS In 9 of 22 patients, cross-clamping of the carotid artery caused a continuous decrease in [oxy-Hb] and [total Hb], and an increase in [deoxy-Hb]. Cyt ox was partially reduced during the clamping. These NIRS responses demonstrated the occurrence of severe hypoxia in the ipsilateral cerebral tissue. These patients showed a marked decrease in the N-20 amplitude of SEP and rCBF. In contrast, the other 13 patients did not show a significant decrease in the cerebral oxygenation state, which showed no remarkable changes in either SEP or in rCBF. CONCLUSIONS NIPS could successfully judge the cerebral oxygenation state noninvasively during carotid surgery and was more sensitive to ischemic crisis than other indirect methods.
  • K Kazumata, S Kuroda, K Houkin, H Abe, K Mitumori CHILDS NERVOUS SYSTEM 12 (3) 161 -165 1996年03月 [査読無し][通常論文]
     
    We investigated the role of ischemic hypoxia in the appearance of re-build-up phenomenon on electroencephalography in childhood moyamoya disease using Tc-99m-hexamethyl propyleneamine oxime and single photon emission computed tomography (Tc-99m-HMPAO SPECT). In the case reported on, critical reduction of regional cerebral blood flow (rCBF) was observed during rebuild-up phenomenon in the bilateral parieto-occipital area where acetazolamide testing revealed severe impairment of the perfusion reserve. On electroencephalography, the rebuild-up phenomenon originated in these areas. In addition, re-build-up phenomenon developed into the entire hemisphere 30 s later, even where the severe ischemia was not observed. These results suggest that rCBF reduction induced by hyperventilation plays a critical role in the appearance of the re-build-up phenomenon, but other factors such as ischemic hypoxia after hyperventilation are also important in the development of this phenomenon.
  • 宝金 清博, ABE Hiroshi, HASHIGUCHI Yuji, SERI Shigemi Neurologia medico-chirurgica = 神経外科 36 (2) 72 -77 1996年02月15日 [査読無し][通常論文]
     
    ラット寒冷損傷モデルを用いて, 脳浮腫がどのように進展するかを検討した. 造影剤の漏出部位とEvans blueの漏出部位の比較をまず行い, その結果, 両者がよく一致することが明らかとなった. 脳浮腫の進展および血液脳関門(BBB)の破綻は, 動物実験用のMRIを用い, 造影剤の漏出により評価した. この結果, 寒冷脳損傷では, 早期に, 損傷の周辺部位よリBBBの障害が起こり, 次第に損傷中心部に波及した. 最終的に, 造影剤は脳室周囲に広がり, 脳室に達し, 脳室系より吸収された. また, BBBの破綻は損傷作成直後ではなく, 数時間後にそのpeakに達することが明らかとなった.
  • Satoshi Kuroda, Kiyohiro Houkin, Hiroshi Abe, Mamoru Tamura Neurologia Medico-Chirurgica 36 (2) 78 -86 1996年02月 [査読無し][通常論文]
     
    The cerebral oxygenation state of five patients was continuously and non- invasively monitored using near-infrared spectroscopy (NIRS) during carotid artery balloon occlusion for comparison with somatosensory evoked potential (SEP) monitoring and regional cerebral blood flow (rCBF) measurements. In patients with marked reduction of rCBF and deteriorated SEP, NIRS demonstrated a continuous decrease in oxy-hemoglobin and total hemoglobin and an increase in deoxy-hemoglobin, indicating the occurrence of severe hypoxia in the ipsilateral hemisphere because of inadequate collateral circulation. In contrast, NIRS showed only a transient (< 2 min) change in these parameters in the patients who showed no remarkable changes in SEP or rCBF, suggesting that well-developed collateral circulation immediately improved cerebral hemodynamics in the ipsilateral hemisphere. These results suggest that NIRS can provide direct and valuable information on cerebral hemodynamic changes during carotid artery balloon occlusion.
  • S Ushikoshi, K Houkin, F Itoh, H Saitoh, M Nozaki, S Kuroda, H Abe JOURNAL OF NEUROSURGERY 84 (2) 269 -271 1996年02月 [査読無し][通常論文]
     
    The authors describe the case of a 69-year-old man with an intracerebral hemorrhage due to rupture of a nontraumatic aneurysm of the middle meningeal artery (MMA). The ipsilateral posterior cerebral artery (PCA) was occluded, and dural anastomoses developed as the main collateral pathway between the MMA and the cortical branch of the PCA, on which the aneurysm was located. It is considered that increased hemodynamic stress to the collateral pathway contributed to the formation of the aneurysm.
  • Tetsuyuki Yoshimoto, Kiyohiro Houkin, Hiroshi Abe Neurologia Medico-Chirurgica 36 (2) 67 -71 1996年02月 [査読無し][通常論文]
     
    Activated leukocytes may be involved in the reperfusion injury of the brain. The expression of intracellular adhesion molecule-1 (ICAM-1) (CD54) and lymphocyte function-associated antigen-1 (LFA-1) (CD11a/CD18) are important for the interaction of activated leukocytes with the brain. Therefore, the expression of ICAM-1 in the cerebral vessels and LFA-1 on leukocytes were examined after reperfusion in a rat four-vessel occlusion model. Model rats underwent reperfusion (15, 30, and 60 min, and 6, 12, and 24 hrs) following 30 minutes of forebrain ischemia. Immunohistological staining for ICAM-1 and LFA-1 was performed in each subgroup. ICAM-1 expression increased after 1-hour reperfusion and persisted on the cerebral microvessels in both the subcortical region and the basal ganglia. Leukocytes stained by LFA-1 were observed in the capillary vessels after 6-hour reperfusion. Increased expression of ICAM-1 and LFA-1 were induced by reperfusion, and this may be important in reperfusion injury of the brain.
  • 吉本 哲之, 宝金 清博, 阿部 弘 新潟医学会雑誌 110 (1) 29 -29 1996年01月 [査読無し][通常論文]
  • 桜木 貢, 三森 研自, 田中 徳彦, 中川 端午, 青樹 毅, 桐山 健司, 黒田 敏, 数又 研, 宝金 清博, 阿部 弘 新潟医学会雑誌 110 (1) 42 -43 1996年01月 [査読無し][通常論文]
  • 野崎 道雅, 牛越 聡, 伊藤 文生, 秋野 実, 斎藤 久寿, 黒田 敏, 宝金 清博, 阿部 弘, 宮坂 和男 新潟医学会雑誌 110 (1) 48 -48 1996年01月 [査読無し][通常論文]
  • 血行再建術後、急性期に出現したhyperperfusion syndromeの脳循環動態の検討
    The Mt Fuji Workshop on CVD 14 156 -161 1996年 [査読無し][通常論文]
  • 特発性Willis動脈輪閉塞症（もやもや病）の臨床・治療
    神経疾患データブック 1996年 [査読無し][通常論文]
  • 神経疾患データブック
    中外医学社 1996年 [査読無し][通常論文]
  • 神経疾患データブック
    中外医学社 1996年 [査読無し][通常論文]
  • 神経疾患データブック
    中外医学社 1996年 [査読無し][通常論文]
  • 神経疾患データブック
    中外医学社 1996年 [査読無し][通常論文]
  • Tetsuyuki Yoshimoto, Kiyohiro Houkin, Hiroshi Abe, Takeshi Kashiwaba Neurologia medico-chirurgica 36 (11) 812 -814 1996年 [査読無し][通常論文]
     
    A 36-year-old male presented with aphasia and right hemiparesis due to the rupture of the larger of two arteriovenous malformations (AVMs) coexisting in the left hemisphere. The two AVMs had completely separate locations and different feeding arteries and draining systems. Two months after resection of the larger AVM and evacuation of the hematoma, carotid angiography showed the residual AVM had spontaneously disappeared. He was discharged without deficits. Change of cerebral hemodynamics after removal of the larger AVM presumably caused the spontaneous regression of the smaller one. Cerebral angiograms should be carefully examined because cerebral hemodynamics may be altered after removal of an AVM. © 1996, The Japan Neurosurgical Society. All rights reserved.
  • K Houkin, S Takikawa, Y Sawamura, H Saitoh, H Abe SURGERY OF THE INTRACRANIAL VENOUS SYSTEM 375 -379 1996年 [査読無し][通常論文]
  • 宝金 清博, 阿部 弘, 米増 祐吉 臨牀と研究 72 (12) 3159 -3168 1995年12月 [査読無し][通常論文]
     
    脳血管障害後遺症の脳代謝賦活薬療法に対するカルナクリン併用効果を検討した.1)全般改善度では,「中等度改善」以上は,12週後で脳代謝賦活薬単独群12.7%,カルナクリン併用群24.1%であった. 2)自覚症状の各症状の中でカルナクリン併用群において「頭痛」「たちくらみ」「首・肩のこり」が単独群に比べて有意に優れていた. 3)精神症候の各症状の中でカルナクリン併用群は「表情の乏しさ」の改善において単独群を上回っていた. 4)有用度では,「かなり有用」以上は,脳代謝賦活薬単独群で16.3%,カルナクリン併用群で29.1%とカルナクリン併用群で優れていた
  • T ISHIKAWA, K HOUKIN, H ABE, M ISOBE, H KAMIYAMA JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY 59 (6) 625 -628 1995年12月 [査読無し][通常論文]
     
    The effectiveness of extracranial-intracranial arterial (EC-IC) bypass surgery for haemodynamic stroke remains controversial. In this study correlation of presurgical and postsurgical cerebral haemodynamics with long term prognosis was evaluated. Twenty eight patients (25 men, three women: mean age 61.4 (SD 8.2) years) with reduced cerebrovascular reserve due to steno-occlusive cerebral vascular disease formed the study group. Measurement of mean hemispheric cerebral blood flow (mCBF) and the cerebral vasodilatory capacity (% mCVR) with an intravenous acetazolamide injection were performed by a xenon-133 inhalation method and SPECT. Patients were treated with EC-IC bypass surgery and measurements of mCBF and % mCVR were made again about one month after surgery. The patients were then observed for a long period (range 27-115 months). During the observation period, four patients experienced subsequent ischaemic strokes. The presurgical and the postsurgical resting mCBF for the affected hemisphere were significantly reduced in the patients with strokes during follow up compared with the values in patients without strokes during follow up (P < 0.03 and 0 01 respectively). The % mCVR of the affected hemisphere was significantly raised after surgery in all patients except one (P < 0.01). The postsurgical change in resting mCBF was not unidirectional. In conclusion, resting mCBF was unchanged and % mCVR was improved after EC-IC bypass surgery in patients with reduced cerebrovascular reserve. The group of patients with a reduced presurgical and postsurgical resting mCBF continue to be a high risk group for subsequent ischaemic stroke seen after EC-IC bypass surgery.
  • 牛越 聡, 伊藤 文生, 斎藤 久寿, 黒田 敏, 宝金 清博, 阿部 弘 脳卒中の外科 = Surgery for cerebral stroke 23 (6) 429 -434 1995年11月30日 [査読無し][通常論文]
  • 中山 若樹, 宝金 清博, 吉本 哲之, 竹田 誠, 徳田 耕一, 柏葉 武, 阿部 弘 脳卒中の外科 = Surgery for cerebral stroke 23 (6) 445 -449 1995年11月30日 [査読無し][通常論文]
  • 中山 若樹, 宝金 清博, 吉本 哲之 脳卒中の外科 23 (6) 445 -449 1995年11月 [査読無し][通常論文]
     
    1)63歳女.右不全片麻痺の一過性虚血発作を経験し,MRI上,左大脳基底核の小窩性梗塞,血管撮影上,左ICA(内頸動脈)の狭窄性キンキング,が認められた.手術所見として,ICA内に限局性粥腫性プラクが認められたので,これがキンキングの原因と考えた.頸動脈内膜切除術及び一次閉鎖を行い,改善を得た. 2)65歳女.右上肢の進行性軽度脱力を示し,MRI上,頭頂葉に斑状梗塞,血管撮影上,左ICAの重症狭窄性キンキング,が認められた.手術所見として,アテローム硬化性変化は軽いが,ICAの延長によるキンキング及び狭窄は重症であった.人工パッチによる血管形成術により改善された
  • 宝金 清博 Medicina 32 (11) 2196 -2198 1995年11月 [査読無し][通常論文]
  • N NAKAYAMA, K ITAMOTO, K TOKUDA, S KAWAGUCHI, T KASHIWABA NEUROLOGICAL SURGERY 23 (11) 1031 -1035 1995年11月 [査読無し][通常論文]
     
    This is a report of an accidental breakaway of a microcatheter during endovascular embolization for cerebral arteriovenous malformation occurring in a 48-year-old female. She had a sudden onset of cerebral herniated signs caused by a large hemorrhage in the region of the right parietal lobe. An angiogram showed a large arteriovenous malformation in the same region. 48 days after emergency evacuation of the hematoma and clipping of a feeding artery, we performed endovascular embolization using a microcatheter as assistant therapy for total removal of the nidus. However, accidentally, the microcatheter broke and migrated to the distal middle cerebral artery. Although we tried to retrieve the catheter using a loop snare, we had no success. We eventually retrieved it by open surgery next day. Fortunately the migrated catheter could be seen through a cortical artery, so after trapping it by using elastic thread and cutting off the artery we were able to extract the migrated catheter. Finally we were able to remove the nidus totally without other complications. Since breaking and migration of a microcatheter is a rare but possible accident, we should be prepared to cope with it appropriately.
  • S KURODA, K HOUKIN, H KAMIYAMA, H ABE, K MITSUMORI CHILDS NERVOUS SYSTEM 11 (10) 584 -590 1995年10月 [査読無し][通常論文]
     
    Regional cerebral blood flow (rCBF) and its reactivity to acetazolamide were analyzed in a series of 15 cases of childhood moyamoya disease, using the xenon-133 inhalation method and single photon emission CT (SPECT). Most of the patients had normal mean hemispheric cerebral blood flow (mCBF), comparable to that of age-matched healthy children. However, they had abnormal rCBF distribution and disturbed reactivity to acetazolamide in the territory of the internal carotid artery, especially in the frontal lobe. Surgical revascularization for the anterior circulation, which consisted of STA-MCA anastomosis and indirect synangiosis, dramatically improved these cerebral hemodynamics in the anterior circulation, including the frontal lobe, and reactivity to acetazolamide in the whole brain. These results suggest that surgical revascularization for the anterior circulation should be carried out in order to improve cerebral hemodynamics as widely as possible, especially in the frontal lobe, and may improve cerebral perfusion reserve in the posterior circulation.
  • 宝金 清博, 数又 研, 高橋 明弘 新薬と臨牀 44 (7) 1183 -1189 1995年07月 [査読無し][通常論文]
     
    精神症候,特に意欲低下,情緒障害において90%以上の高い改善率を認めた.脳梗塞後遺症,脳出血後遺症の何れにおいても高い改善率を示した.罹病期間が3ヵ月未満の症例に高い有効性を認めた.本剤に起因していると考えられる副作用や臨床検査値の変動は1例も認められず,安全性が確認された
  • T ISHIKAWA, K HOUKIN, H ABE SURGICAL NEUROLOGY 43 (5) 508 -509 1995年05月 [査読無し][通常論文]
  • T ISHIKAWA, H KAMIYAMA, K HOUKIN, A TAKAHASHI, Y IWASAKI, H ABE SURGICAL NEUROLOGY 43 (5) 500 -506 1995年05月 [査読無し][通常論文]
     
    BACKGROUND When we encounter an inaccessible internal carotid artery lesion, we sacrifice the inaccessible internal carotid artery and, at the same time, establish a high-flow extracranial-to-intracranial arterial bypass (EC-IC bypass). Questions and concerns remain, however, about the dynamics of the patient's cerebral blood flow postoperatively. METHOD Ten hemispheres from 9 patients had received radial artery grafting as an EC-IC bypass with the simultaneous obstruction of the internal carotid artery. Their mean hemispheric cerebral blood flow (mCBF) was measured longitudinally during 1 year after surgery by single photon emission computed tomography (SPECT) and a (133)Xenon-inhalation method. RESULTS The serial mCBF values of the five hemispheres with giant aneurysm or paracavernous meningioma were attenuated during the postoperative 3-month period and then returned to the normal range. In only one case did the postoperative reduction of mCBF fail to return to normal; in addition there was a memory disturbance that had developed and progressed, Five hemispheres with the ICA stenosis did not present reduction in mCBF. In three out of five hemispheres, transient elevation of mCBF at 1 month after surgery was observed. CONCLUSIONS We conclude from our study that the internal carotid artery can be safely substituted by the radial artery graft from the view point of cerebral blood flow.
  • 竹田 誠, 宝金 清博, 阿部 弘 脳卒中の外科 23 (3) 211 -214 1995年05月 [査読無し][通常論文]
     
    後頭蓋窩硬膜動静脈瘻に対する経静脈的塞栓術は低侵襲かつ根治性の高い治療法であるが,シャント量の多い症例では,あらかじめ経静脈的塞栓術を行うことにより合併症を避けることが重要である.罹患静脈洞の近位および遠位両端での閉塞がなければtransfemoral approachが可能であり,更に低侵襲な治療が可能となる.本法は後頭蓋窩硬膜動静脈瘻の治療の第一選択と考えられる
  • TAKAHASHI Akihiro, KAMIYAMA Hiroyasu, HOUKIN Kiyohiro, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 35 (4) 231 -237 1995年04月15日 [査読無し][通常論文]
     
    Indirect revascularization procedures centered on the parietal region, such as encephalo-myo-arterio-synangiosis (parietal synangiosis) and direct procedures centered on the frontal region using both the anterior and the posterior branches of the superficial temporal artery (STA), such as STA to middle cerebral artery anastomosis combined with encephalo-duro-arterio-myo-synangiosis (frontal anastomosis) were compared in childhood moyamoya disease patients. The parietal synangiosis group consisted of 10 sides in five patients, and the frontal anastomosis group consisted of 30 sides in 15 patients. The development of postoperative collateral circulation was assessed by external carotid angiography, the neurological outcome was monitored for 2 years after surgery, and the intelligence quotient (IQ) was measured at least 6 months after surgery. Frontal anastomosis achieved superior results compared to the parietal synangiosis assessed by development of collateral circulation, in particular to the orbitofrontal artery, the prefrontal artery, and the precentral artery (p < 0.01), and reduction in the incidence of ischemic attacks, such as transient ischemic attacks (p < 0.05). The mean IQ in the frontal anastomosis group was higher than that in the parietal synangiosis group. Vascular reconstruction centered on the frontal region utilizing both the anterior and posterior branches of the STA is more efficacious than only synangiosis centered on the parietal region.
  • 脳梗塞急性期におけるThromboxan合成酵素阻害剤の効果 OKY-046(Sodium Ozagrel)は脳梗塞急性期,血小板活性を抑制する
    宝金 清博, 阿部 弘, 岩崎 喜信 診療と新薬 32 (4) 707 -713 1995年04月 [査読無し][通常論文]
  • S KURODA, H KAMIYAMA, M ISOBE, K HOUKIN, H ABE, K MITSUMORI CHILDS NERVOUS SYSTEM 11 (4) 214 -219 1995年04月 [査読無し][通常論文]
     
    ''Re-build-up'' phenomenon, induced by hyperventilation, is a characteristic finding on electro-encephalogram (EEG) in children with moyamoya disease, and suggests close correlation with cerebral ischemic attack. Its mechanism, however, remains obscure. In this study, we examined the relationship between the cerebral hemodynamics and this phenomenon before and after vascular reconstructive surgery. Regional cerebral blood flow and its reactivity to acetazolamide were analyzed using the xenon-133 inhalation method and single photon emission computed tomography in six children with moyamoya disease. These results were compared with the EEG. Regional vasoreactivity to acetazolamide was significantly lower in ''re-build-up''-positive regions than in ''re-build-up''-negative regions. Postoperative studies revealed a significant improvement of reactivity in the regions where the ''re-build-up'' phenomenon disappeared after surgery. These results suggest that the ''re-build-up'' phenomenon represents a focal reduction of the cerebral perfusion reserve in moyamoya disease.
  • 黒田 敏, 宝金 清博, 上山 博康 小児の脳神経 20 (2) 67 -72 1995年04月 [査読無し][通常論文]
     
    小児モヤモヤ病15例を対象に,133-xenon吸入法によるSPECTを用いて,局所脳血流量に加えてacetazolamide反応性を測定し,健常小児の結果と比較した.術前,半球平均血流量の低下は4例にのみ認められた.対照的に,12例で前頭葉,側頭葉における脳血流量の低下を認め,また,全例でacetazolamide反応性の障害を認めた.脳血行再建術後,安静時の脳血流分布は前頭葉も含めて正常化した.また,acetazolamide反応性も前頭葉,側頭葉,頭頂葉にとどまらず,大脳基底核,後頭葉においても明らかな改善を認めた
  • IMAMURA Hiroyuki, IWASAKI Yoshinobu, HIDA Kazutoshi, CHO Ki Hong, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 35 (3) 144 -147 1995年03月15日 [査読無し][通常論文]
     
    Intraoperative spinal sonography was used during cervical anterior approach procedures for cervical discectomy and osteophytectomy to demonstrate spinal pulsation, the protruded disc or osteophyte, the anterior subarachnoid space, and the spinal cord. Spinal pulsation was recognized in some cases before removal of the disc but the anterior subarachnoid space and spinal cord could not be observed. However, the latter were more clearly observed during removal of the disc and could be seen after total removal of the disc and osteophyte. This method allows confirmation of decompression and pulsation of the spinal cord without cutting and removal of the posterior longitudinal ligament.
  • 鎌田 恭輔, 宝金 清博, 阿部 弘 CI研究 17 (1) 59 -67 1995年03月 [査読無し][通常論文]
     
    実験例412例を対象にして,空間解像力の限界,各metaboliteの緩和時間の病変による影響について分析した.空間解像力の限界として,評価できるS/N比を得る為には,最低8cm3の大きさが必要であることを明らかにした.緩和時間も病変により,又,エコー時間の長さによってもCrのピークが変化する事実を示して,外基準を工夫すべきであると主張している.更に現在のパラメータでは限られた物質をとらえることができるのみで,短いエコー時間で測定する方法を提案している
  • K HOUKIN, K KAMADA, Y SAWAMURA, Y IWASAKI, H ABE, T KASHIWABA NEURORADIOLOGY 37 (2) 99 -103 1995年02月 [査読無し][通常論文]
     
    We investigated metabolic changes in brain tumours following treatment, using proton magnetic resonance spectroscopy. In meningiomas, effective therapeutic embolisation led to an acute increase in lactate. In radiosensitive tumours such as malignant lymphoma, a decrease in lactate and in in crease in N-acetyl-aspartate occurred after radiotherapy, which preceded changes observed on magnetic resonance imaging. On the other hand, no significant changes in spectral patterns were observed in malignant gliomas resistant to therapy. Tissue characterisation of brain tumours by spectral patterns on proton magnetic resonance spectroscopy remains controversial. However, we have shown it to be sensitive to metabolic changes following treatment, which may reflect the efficacy of the therapy.
  • KAMADA Kyousuke, HOUKIN Kiyohiro, HIDA Kazutoshi, IWASAKI Yoshinobu, ABE Hiroshi Neurologia medico-chirurgica = 神経外科 35 (1) 1 -7 1995年01月15日 [査読無し][通常論文]
     
    The serial changes in metabolism and histology during the first 24 hours in the cold-injury trauma rat brain model were investigated by proton magnetic resonance (MR) imaging and high-resolution proton MR spectroscopy. Edema developed extensively via the corpus callosum in the ipsi- and contralateral hemispheres during observation as shown by gradually increased signal intensity on proton MR images. Proton MR spectroscopy showed increased levels of acetate (Ace), lactate (Lac), and glutamine (Glmi) 1 hour after lesion formation. The elevated Glmi level slightly decreased, the level of alanine (Ala) increased substantially, and that of N-acetyl-aspartate (NAA) decreased markedly after 24 hours. Increased Lac, Ace, and Ala might reflect anaerobic glycolysis associated with mitochondrial dysfunction, while decreased Glmi and NAA reveal brain tissue breakdown. The relationship between brain edema and tissue viability can be analyzed in detail using this simple traumatic model and MR techniques which will be useful in the development of therapeutic agents for brain injury.
  • T NOHJOH, K HOUKIN, A TAKAHASHI, H ABE NEUROSURGERY 36 (1) 180 -182 1995年01月 [査読無し][通常論文]
     
    A CASE OF a ruptured dissecting aneurysm of the vertebral artery, which was detected by repeated angiography (third time), is reported. A 59-year-old woman, whose preoperative angiogram showed no abnormality, underwent a bifrontal craniotomy and the total removal of the right frontal glioma. However, a subarachnoid hemorrhage was detected by computed tomography the day after the surgery. Conventional angiography performed on the same day revealed no definite abnormality. Nevertheless, 9 days after surgery, a second subarachnoid hemorrhage occurred. The dissecting aneurysm of the vertebral artery was revealed by angiography. It is important to suspect this type of aneurysm as a differential diagnosis when the cause of the subarachnoid hemorrhage is not clarified in the first angiogram.
  • 断裂し脳内に遺残したmicrocatheterを手術摘出した1例.
    脳神経外科 23 1031 -5 1995年 [査読無し][通常論文]
  • 脳梗塞急性期におけるThromboxan合成酵素阻害剤の効果−OKY-046(Sodium Ozagrel)は脳梗塞急性期、血小板活を抑制する−
    診療と新薬 32 15 -21 1995年 [査読無し][通常論文]
  • 脳血管障害患者に対するPropentofylline（ヘキストールR錠）の効果−8施設における共同研究−
    新薬と臨床 32 15 -21 1995年 [査読無し][通常論文]
  • Tatsuru Nohjoh, Kiyohiro Houkin, Akihiro Takahashi, Hiroshi Abe Neurosurgery 36 (1) 180 -183 1995年 [査読無し][通常論文]
     
    A CASE OF a ruptured dissecting aneurysm of the vertebral artery, which was detected by repeated angiography (third time), is reported. A 59-year-old woman, whose preoperative angiogram showed no abnormality, underwent a bifrontal craniotomy and the total removal of the right frontal glioma. However, a subarachnoid hemorrhage was detected by computed tomography the day after the surgery. Conventional angiography performed on the same day revealed no definite abnormality. Nevertheless, 9 days after surgery, a second subarachnoid hemorrhage occurred. The dissecting aneurysm of the vertebral artery was revealed by angiography. It is important to suspect this type of aneurysm as a differential diagnosis when the cause of the subarachnoid hemorrhage is not clarified in the first angiogram. Copyright © by the Congress of Neurological Surgeons.
  • Diagnosis of Moyamoya Disease With Magnetic Resonance Angiography
    MosbyYear Book of Neuroradiology 1995 1995年 [査読無し][通常論文]
  • Functional Neurosurgeical Simulation With Brain Surface Magnetic Resonance Images and Magnetoencephalography
    Year Book of Neurology and Neurosurgery 1995年 [査読無し][通常論文]
  • Willis動脈輪閉塞症の過呼吸負荷99mTc-HMPAO SPECT Re-build up現象の出現メカニズムについて
    数又 研, 黒田 敏, 宝金 清博 CI研究 16 (4) 335 -342 1994年12月 [査読無し][通常論文]
  • K HOUKIN, T AOKI, A TAKAHASHI, H ABE STROKE 25 (11) 2159 -2164 1994年11月 [査読無し][通常論文]
     
    Background and Purpose Present criteria for the diagnosis of moyamoya disease emphasize the use of conventional cerebral angiography as an indispensable requirement. However, magnetic resonance angiography (MRA) is fast becoming a reliable diagnostic modality for occlusive cerebrovascular diseases. The purpose of this study is to assess the accuracy of MRA compared with conventional angiography. Methods Thirty-nine patients (23 children and 16 adults) with moyamoya disease confirmed by conventional angiography (78 side views) were examined with MRA (three-dimensional time-of-flight technique with a 256x256 or 256x512 matrix). T-1- and T-2-weighted MR images were also taken. Results The stenotic or occlusive change at the carotid fork was clearly revealed by MRA, which correlated well with conventional angiography (83% good correlation, 17% overestimation). Apparent moyamoya vessels were clearly shown as a signal void on the MR image and fine unusual vessels on MRA, particularly in children with moyamoya disease (82%). However, small moyamoya vessels were poorly shown on both MR images and MRA, particularly in adults (63%). Conclusions MRA can be an alternative to conventional angiography in typical moyamoya disease cases (the bilateral type of moyamoya disease in children). However, in the early or the end stages of moyamoya disease, diagnosis by means of MRA should be carefully evaluated.
  • 成人もやもや病に対する血行再建術
    宝金 清博 日本脳神経外科学会総会抄録集 53回 35 -35 1994年10月 [査読無し][通常論文]
  • 脳動脈瘤破裂術中major ruptureの原因と対策
    宝金 清博 日本脳神経外科学会総会抄録集 53回 311 -311 1994年10月 [査読無し][通常論文]
  • 大槻 浩之, 板本 孝治, 徳田 耕一, 鎌田 恭輔, 磯西 克佳, 下山 三夫, 小岩 光行, 川口 進, 柏葉 武, 宝金 清博, 阿部 弘, 菊池 陽一, 阿部 悟, 宮坂 和男 新潟医学会雑誌 = 新潟医学会雑誌 108 (10) 781 -781 1994年10月 [査読無し][通常論文]
  • 黒田 敏, 宝金 清博, 阿部 弘 脳卒中 16 (5) 374 -379 1994年10月 [査読無し][通常論文]
     
    脳主幹動脈の閉塞性病変を合併したくも膜下出血の症例に対して,脳動脈瘤クリッピングと同時に行う脳血行再建術の意義について検討した。頸動脈あるいは中大脳動脈に高度狭窄,閉塞を合併していた5症例を対象とした。頸動脈閉塞症の2例ではWillis動脈輪を介する良好な側副血行路が認められたため,脳血行再建術は行わなかった。症候性脳血管攣縮は生じなかった。中大脳動脈狭窄症1例で脳血行再建術を行わなかったところ,1週間後,その領域に広範な脳梗塞が出現し重篤な神経症状が残存した。ほかの2例に対しては脳動脈瘤クリッピングと同時に浅側頭-中大脳動脈吻合術を行ったところ,症候性脳血管攣縮は生じず経過良好であった
  • 宝金 清博, 中山 若樹, 鎌田 恭輔 映像情報Medical 26 (20) 1157 -1160 1994年10月 [査読無し][通常論文]
  • A TAKAHASHI, T USHIKI, K ABE, K HOUKIN, H ABE ARCHIVES OF HISTOLOGY AND CYTOLOGY 57 (4) 341 -350 1994年10月 [査読無し][通常論文]
     
    The three-dimensional arrangement of the medial smooth muscle cells of human major intracranial arteries was studied by scanning electron microscopy after removal of extracellular connective tissue matrices with a KOH-collagenase digestion method. In the straight portion of the major arteries, the smooth muscle cells were arranged roughly circularly, whereas the arrangement was somewhat random in the vertebral, basilar, and internal carotid arteries. Groups of the longitudinal muscle cells were also found in the vertebral, basilar, and internal carotid arteries, but were absent in the anterior, middle, and posterior cerebral arteries. At a higher magnification, smooth muscle cells in these arteries formed anastomosed bundles about 5-30 mu m in diameter. The smooth muscle cells in the dichotomous branching and the uniting portions were arranged circularly, but multidirectionally and longitudinally oriented smooth muscle cell groups were present in the facial and dorsal walls of the forked vessels. These multidirectional muscle cell groups were small in number in the portion where the major arteries gave off lateral branches at a right angle. So called ''medial defects'' were found at the crotch of the bifurcating region in two cases out of four. Smooth muscle cells near the defects tapered off toward the center of the defect where internal elastic lamina with oval fenestrations were exposed. However, no special arrangement of the smooth muscle cells was observed around the ''medial defects'' as compared with that in the ordinary bifurcating region.
  • A TAKAHASHI, T USHIKI, K ABE, K HOUKIN, H ABE ARCHIVES OF HISTOLOGY AND CYTOLOGY 57 (4) 331 -339 1994年10月 [査読無し][通常論文]
     
    Three-dimensional cytoarchitectures of the periendothelial cells of human cerebral venous vessels as well as a scalp vein were studied by scanning electron microscopy after removal of extra-cellular connective tissue matrices with a KOH-collagenase digestion method. Postcapillary venules appeared covered with pericytes, while collecting venules had stellate periendothelial cells which formed a basket-like network around the vessel wall. As the size of the venous vessels increased, the stellate periendothelial cells became flat in shape and dense in arrangement. Although they had some characteristics similar to the smooth muscle cells in surface structure, no spindle-shaped smooth muscle cells were recognizable even in the superficial cerebral veins. On the other hand, the scalp vein was densely covered with spindle-shaped smooth muscle cells running circularly. These findings suggest that the cerebral venous vessels cannot constrict strongly as compared with the scalp vein, but probably regulate blood volume by mildly changing the caliber of the vessels.
  • MR angiographyによる脳動脈瘤の検討 破裂動脈瘤および未破裂動脈瘤の診断における問題点と限界
    石井 伸明, 宝金 清博, 青樹 毅 CI研究 16 (3) 231 -236 1994年09月 [査読無し][通常論文]
  • K HOUKIN, N TANAKA, A TAKAHASHI, H KAMIYAMA, H ABE, N KAJII CHILDS NERVOUS SYSTEM 10 (7) 421 -425 1994年09月 [査読無し][通常論文]
     
    The authors report four cases of familial occurrence of moyamoya disease. Although the pathogenesis of moyamoya disease is not clear, there is extensive evidence that this disease has a tendency to show multifactorial inheritance. Therefore, a screening test for those at high risk, i.e., who have a moyamoya patient among their blood relatives, is clinically important. Magnetic resonance angiography (MRA) successfully revealed abnormal findings specific to moyamoya disease in members of the four probands families. MRA is a powerful and noninvasive way of detecting individuals at high risk of developing moymoya disease.
  • 133Xe SPECTによる脳循環予備能測定でCO2負荷及び、Acetazolamide負荷での反応性の差異について
    田中 徳彦, 三森 研自, 桜木 貢, 本宮 峯生, 中川 端午, 黒田 敏, 宝金 清博, 阿部 弘 核医学 31 (7) 760 -760 1994年07月 [査読無し][通常論文]
  • 高橋 明弘, 上山 博康, 宝金 清博 脳卒中の外科 22 (4) 307 -310 1994年07月 [査読無し][通常論文]
     
    真後ろを向いた内頸動脈瘤1例,真の後交通動脈瘤2例,脳底動脈瘤5例(うち,急性期3例)に対して,本法を用いて手術を行った。全例,十分なworking areaが得られ,clipping可能であった
  • 高橋 明弘, 宝金 清博, 上山 博康 脳卒中 16 (3) 201 -205 1994年06月 [査読無し][通常論文]
     
    モヤモヤ病患者18例と動脈硬化性の脳主幹動脈閉塞栓症患者12例の脳表髄液,椎間板障害患者15例の腰椎穿刺にて得た髄液のbFGFをEIAで測定した。1)モヤモヤ病では18例中16例にbFGFが検出され,平均値85.5pg/mlであった。動脈硬化性の脳主幹動脈閉塞栓症では12例中5例で検出され,平均値は検出限界(10pg/ml)以下であった。椎間板障害ではbFGFは検出されなかった。2)モヤモヤ病では3〜4週後に行った対側の手術で得た脳表髄液中にもbFGFが検出された。3)髄液中にbFGFが検出されたモヤモヤ病症例では,間接血行再建術も有効であった。4)頭蓋内でのbFGFの異常な持続的な産生が,モヤモヤ病患者のクモ膜下腔で生じるWillis輪の狭窄閉塞性変化やtrans-dural anastomosisに関与している可能性が示唆された
  • MRIは進化する MRA,MRSのめざすもの
    宝金 清博 新医療 21 (6) 64 -67 1994年06月 [査読無し][通常論文]
  • 青樹 毅, 宝金 清博, 阿部 弘 脳卒中の外科 22 (3) 177 -180 1994年05月 [査読無し][通常論文]
  • K KAMADA, K HOUKIN, K HIDA, H MATSUZAWA, Y IWASAKI, H ABE, T NAKADA MAGNETIC RESONANCE IN MEDICINE 31 (5) 537 -540 1994年05月 [査読無し][通常論文]
     
    Localized proton nuclear magnetic resonance (NMR) spectroscopy of human brain in two common focal pathologies producing brain edema (peritumor edema and acute edematous ischemic stroke) was performed utilizing point resolved spectroscopy (PRESS). The spectra obtained from the pathological tissues were characterized by a reduced N-acetyl-aspartate, (NAA) to total creatine (Cr) ratio (NAA/Cr) and high level of lactate. While the spin lattice relaxation time (T-1) of the main metabolite resonances, namely, those of NAA, Cr, and choline containing compounds (Cho), showed values similar to those of normal brain, the spin-spin relaxation time (T-2) of these metabolites exhibited a dramatic shortening in pathological tissues. Serial postoperative measurements of T-2 in two patients with peritumor edema showed a gradual recovery of the T-2 shortening corresponding to improvement of the edema. The majority of localized spectroscopy studies in humans is performed using a sequence which utilizes spin echo signals with a fined single echo time. Hence, the signal intensities of the metabolite resonances obtained are inherently T-2 dependent. The current study underscores that cautious interpretation of clinical data with respect to metabolite quantification is warranted.
  • MRによる流れの分析 MRAによる血行動態を中心に
    宝金 清博 日本医学放射線学会雑誌 54 (3) 75 -75 1994年03月 [査読無し][通常論文]
  • 脳ドック
    阿部 弘, 宝金 清博 実験治療 634 (634) 44 -46 1994年03月 [査読無し][通常論文]
  • A TAKAHASHI, H KAMIYAMA, K HOUKIN, H ABE JOURNAL OF NEUROSURGERY 80 (2) A400 -A400 1994年02月 [査読無し][通常論文]
  • K KAMADA, K HOUKIN, T AOKI, M KOIWA, T KASHIWABA, Y IWASAKI, H ABE NEURORADIOLOGY 36 (2) 104 -106 1994年02月 [査読無し][通常論文]
     
    The pathogenesis of delayed sequelae of carbon monoxide (CO) exposure is still unknown. We repeatedly examined a 55-year-old woman with the interval form of CO poisoning, using proton magnetic resonance spectroscopy (MRS). When the clinical picture was severe, MRS revealed markedly lowered N-acetyl-asparatate (NAA)/creatine and phosphocreatine (Cr) ratio and slightly increased choline containing compounds (Cho)/Cr ratio. Subsequently, NAA and Cho/Cr ratio tended to return to normal, reflecting clinical improvement. Proton MRS shows the previously unrecognised neuronal activity in CO poisoning and precisely reflects the severity of symptoms. We stress the superiority of proton MRS over the conventional radiological examinations in CO poisoning.
  • 黒田 敏, 上山 博康, 宝金 清博 脳卒中の外科 22 (1) 9 -14 1994年01月 [査読無し][通常論文]
     
    過去6年間に頸動脈狭窄患者63例73側に顕微外科的頸動脈内膜剥離術を脳波及び体性間隔誘発電位によるモニタリング下で行った。周術期合併症は7例に見られ,脳梗塞4例,一過性舌下神経麻痺1例,激症肝炎1例,及び肺炎1例であった。2例では,内シャントの一過性閉塞による血栓閉塞症が重篤な神経症状を来した。頸動脈狭窄が極めて高度で,灌流予備力が減少していた2例では,血行力学的虚血は神経学的増悪を起こさないか,少し増悪させた。平均24.5ヵ月の追跡期間中,4例で脳梗塞(3例では同側頸動脈領域,1例では椎骨脳底動脈領域)が見られた。別の1例は心筋梗塞により死亡した
  • 宝金 清博, 高橋 明弘, 阿部 弘 新潟医学会雑誌 108 (1) 47 -47 1994年01月 [査読無し][通常論文]
  • 板本 孝治, 石井 伸明, 内沢 隆充, 下山 三夫, 磯西 克佳, 小岩 光行, 川口 進, 柏葉 武, 上山 博康, 宝金 清博, 阿部 弘 新潟医学会雑誌 = 新潟医学会雑誌 108 (1) 71 -72 1994年01月 [査読無し][通常論文]
  • 高橋 明弘, 宝金 清博, 阿部 弘, 上山 博康, 野村 三起夫 新潟医学会雑誌 108 (1) 68 -68 1994年01月 [査読無し][通常論文]
  • 石井 伸明, 板本 孝治, 内沢 隆充, 下山 三夫, 小岩 光行, 川口 進, 柏葉 武, 青樹 毅, 宝金 清博, 阿部 弘 新潟医学会雑誌 = 新潟医学会雑誌 108 (1) 73 -73 1994年01月 [査読無し][通常論文]
  • 脳卒中の外科 22 307 -310 1994年 [査読無し][通常論文]
  • 宝金 清博, 鎌田 恭輔, 阿部 弘 最新医学 49 (1) 47 -54 1994年01月 [査読無し][通常論文]
  • K HOUKIN, T AOKI, A TAKAHASHI, H ABE, M KOIWA, T KASHIWABA ACTA NEUROCHIRURGICA 128 (1-4) 132 -136 1994年 [査読無し][通常論文]
     
    The purpose of this study was to evaluate the high resolution magnetic resonance angiography (MRA) as pre-operative angiography for the detection of ruptured cerebral aneurysms. MRA was performed on 1.5 tesla system using the 3-dimensional time of flight (3D-TOF) method. The field of view was 16 cm or 20 cm and matrix size was 192 x 256 or 256 x 512. Twenty patients with ruptured cerebral aneurysms and 35 cases of non-ruptured aneurysms (incidental aneurysm) examined by both conventional angiography and MRA were included in this study. All the ruptured aneurysms were operated on based on the information obtained from conventional angiography and MRA. Aneurysms smaller than 3 mm in size were difficult to visualize on MRA. However, most ruptured aneurysms were clearly visualized because they were generally larger than 3 mm. The image quality of MRA was satisfactory for planning surgery. Screening for non-ruptured cerebral aneurysms using MRA is controversial. However, in ruptured aneurysms larger than 3 mm in size, MRA clearly revealed the aneurysm, and MRA findings were informative enough to plan surgery. It is concluded that this non-invasive examination can be selected as a first-choice examination especially in cases of ruptured aneurysm.
  • K KAMADA, K HOUKIN, Y IWASAKI, H ABE ACTA NEUROCHIRURGICA 60 491 -493 1994年 [査読無し][通常論文]
     
    We clearly represented brain structures of rats and permitted a rapid assessment of water gradient of the brain edema by cortical freezing utilizing a high-field (7T) proton magnetic resonance imaging (MRI). The typical time course of vasogenic edema and the efficacy of AVS;1,2-bis (nicotinamide)-propane upon the edema were presented. Twelve rats with edema induced by cortical freezing were divided into two groups; one group of animals received 0.5 ml of physiological saline with 100 mg (/kg) AVS every eight hours intraperitoneally. The other group of untreated animals received only saline. One three, six, 12, and 24 hours after lesion production, the profiles of edema fluid spreading and the maximum signal intensity (MI) of some regions of interest (ROI) were assessed by T2 weighted images (TE = 70 ms, TR = 3500 ms). One hour after lesion production in the untreated group, a low heterogeneous intensity area was seen mainly in the primarily injured cortex. Two hours later, the margin of the lesion gradually increased in intensity and MI of ROIs around the lesion also gradually increased. Twenty-four h after lesion production edema extended contralaterally via corpus callosum. AVS reduced edema fluid spreading beginning from about six hours after lesion production. The MIs of the AVS treated group were significantly lower than in the untreated group (p < 0.01). We conclude that sequential observation of edema using MRI is a quite practical technique for evaluation of the efficacy of any therapeutic agent.
  • Kyousuke Kamada, Kiyohiro Houkin, Yoshinobu Iwasaki, Hiroshi Abe, Takeshi Kashiwaba Neurologia Medico-Chirurgica 34 (10) 676 -681 1994年 [査読無し][通常論文]
     
    The metabolism of the brain was investigated in eight patients with peritumoral edema, six patients with ischemic stroke, and 28 normal controls using proton magnetic resonance (MR) spectroscopy. The MR studies were performed using a 1.5-T whole-body imaging and spectroscopy system with a 1500-msec repetition time (TR) and a 270-msec echo time (TE). The peak areas for TV-acetyl-aspartate (NAA), choline-containing compounds (Cho), creatine and phosphocreatine (Cr), and lactate (Lac) were measured, and the NAA/Cr, Cho/Cr, and Lac/Cr ratios were calculated. To quantify and compare the serial spectra, relaxation effects were investigated by acquisitions at two different points (TRs or TEs) and by monoexponential fitting. The normal NAA/Cr and Cho/Cr ratios were 2.76 and 1.09, respectively. Lac could not be identified in normal brains. In ischemic stroke and peritumoral edema, significantly increased Lac/Cr and decreased NAA/Cr ratios were observed. Resolution of peritumoral edema was associated with normalized NAA/Cr ratio and disappearance of Lac. The T1 relaxation times of the metabolites were similar in normal brain and peritumoral edema, but the T2 values were significantly shortened. Serial measurements of T2 values in two patients with peritumoral edema showed gradual normalization corresponding to improvement of the edema. To absolutely quantify metabolite concentrations in edema, changes in relaxation times should be considered. Copyright © 1994, The Japan Neurosurgical Society. All rights reserved.
  • T. Nakakoshi, H. Matsuzawa, K. Houkin, K. Miyasaka, T. Arimoto Radiation Medicine - Medical Imaging and Radiation Oncology 12 (3) 139 -142 1994年 [査読無し][通常論文]
     
    Proton magnetic resonance spectroscopy (1H-MRS) is thought to be more sensitive to radiation damage of the brain than magnetic resonance imaging (MRI). We presented performed 1H-MRS using the stimulated echo acquisition method (STEAM) for non-tumor sites in 2 patients who received radiation therapy for their brain tumors. In the first case up to 45 days after radiation, we could observe a normal spectra presented three major peaks of N-acetyl aspartate (NAA), creatine and phosphocreatine (Cr), and choline (Cho). In the second case who had radiation necrosis 1 year after radiation, the spectra showed a decrease in the all peaks beneath the noise level.
  • K HOUKIN, A TAKAHASHI, H ABE SURGICAL NEUROLOGY 41 (1) 16 -18 1994年01月 [査読無し][通常論文]
     
    The authors present a method of the surgical dissection of the interhemispheric fissure based on high resolution magnetic resonance images (MRI). The dissection of the interhemispheric space is not easy because the brain surfaces are tightly adherent in the interhemispheric fissure. In other words, in the interhemispheric fissure, the apices of the gyri are touching and are tightly bound together. This microanatomy is clearly revealed using high resolution three-dimensional MRI. The principle of the usage of the brain retractor is to apply them symmetrically in order to give an even tension to both sides. However, in the interhemispheric dissection, this principle should be slightly modified. Namely the brain retractors should be placed on the crest of gyri on both sides to avoid the injury of small arteries located in the sulci by the edge of the brain retractors.
  • 川口 進, 鎌田 恭輔, 大槻 浩之, 板本 孝治, 徳田 耕一, 磯西 克佳, 下山 三夫, 柏葉 武, 黒田 徹, 宝金 清博 日本てんかん学会プログラム・予稿集 0 (28) 92 -92 1994年 [査読無し][通常論文]
  • Kyousuke Kamada, Kiyohiro Houkin, Yoshinobu Iwasaki, Hiroshi Abe, Takeshi Kashiwaba Neurologia Medico-Chirurgica 34 (10) 676 -681 1994年 [査読無し][通常論文]
     
    The metabolism of the brain was investigated in eight patients with peritumoral edema, six patients with ischemic stroke, and 28 normal controls using proton magnetic resonance (MR) spectroscopy. The MR studies were performed using a 1.5-T whole-body imaging and spectroscopy system with a 1500-msec repetition time (TR) and a 270-msec echo time (TE). The peak areas for TV-acetyl-aspartate (NAA), choline-containing compounds (Cho), creatine and phosphocreatine (Cr), and lactate (Lac) were measured, and the NAA/Cr, Cho/Cr, and Lac/Cr ratios were calculated. To quantify and compare the serial spectra, relaxation effects were investigated by acquisitions at two different points (TRs or TEs) and by monoexponential fitting. The normal NAA/Cr and Cho/Cr ratios were 2.76 and 1.09, respectively. Lac could not be identified in normal brains. In ischemic stroke and peritumoral edema, significantly increased Lac/Cr and decreased NAA/Cr ratios were observed. Resolution of peritumoral edema was associated with normalized NAA/Cr ratio and disappearance of Lac. The T1 relaxation times of the metabolites were similar in normal brain and peritumoral edema, but the T2 values were significantly shortened. Serial measurements of T2 values in two patients with peritumoral edema showed gradual normalization corresponding to improvement of the edema. To absolutely quantify metabolite concentrations in edema, changes in relaxation times should be considered. Copyright © 1994, The Japan Neurosurgical Society. All rights reserved.
  • H KAMIYAMA, A TAKAHASHI, K HOUKIN, S MABUCHI, H ABE, T FUKUSHIMA, AL DAY NEUROSURGERY 33 (6) 1109 -1110 1993年12月 [査読無し][通常論文]
     
    VISUALIZATION OF THE ostium of an arteriotomy by staining it blue with methylrosaniline chloride (pyoctaninum blue) in anastomotic bypass surgery for children with moyamoya disease is described. This technique enables surgeons to create an anastomosis more precisely and quickly. No adverse effects were observed.
  • 青樹 毅, 宝金 清博, 阿部 弘 日本臨床 51 (増刊 CT, MRI時代の脳卒中学(上)) 125 -131 1993年11月 [査読無し][通常論文]
  • ラット凍結損傷モデル(血管原性脳浮腫)の血液脳関門破綻と浮腫液の動態(MRIによる検討)
    宝金 清博 脳循環代謝 5 (1) 109 -109 1993年11月 [査読無し][通常論文]
  • A TAKAHASHI, Y SAWAMURA, K HOUKIN, H KAMIYAMA, H ABE NEUROSCIENCE LETTERS 160 (2) 214 -216 1993年10月 [査読無し][通常論文]
     
    The level of basic fibroblast growth factor (bFGF) in cerebrospinal fluid (CF) was measured by an EIA in 15 patients with moyamoya disease, in 11 patients with atherosclerotic occlusive disease, and in 8 patients with spinal disc disease. In the moyamoya patients, bFGF was found in 13 out of 15 CF samples with a mean value of 101 pg/ml. However, bFGF was detected in only 4 out of 11 patients with atherosclerotic disease with a mean of 8 pg/ml. In all the patients with disc disease, bFGF was not detected. The elevated level of bFGF may play a crucial role in the pathogenesis of moyamoya disease.
  • A TAKAHASHI, Y SAWAMURA, K HOUKIN, H KAMIYAMA, H ABE NEUROSCIENCE LETTERS 160 (2) 214 -216 1993年10月 [査読無し][通常論文]
     
    The level of basic fibroblast growth factor (bFGF) in cerebrospinal fluid (CF) was measured by an EIA in 15 patients with moyamoya disease, in 11 patients with atherosclerotic occlusive disease, and in 8 patients with spinal disc disease. In the moyamoya patients, bFGF was found in 13 out of 15 CF samples with a mean value of 101 pg/ml. However, bFGF was detected in only 4 out of 11 patients with atherosclerotic disease with a mean of 8 pg/ml. In all the patients with disc disease, bFGF was not detected. The elevated level of bFGF may play a crucial role in the pathogenesis of moyamoya disease.
  • 3次元MRI法(立体imaging)による手術simulationの有用性
    宝金 清博 日本脳神経外科学会総会抄録集 52回 208 -208 1993年09月 [査読無し][通常論文]
  • 青樹 毅, 宝金 清博, 上山 博康 Neurological Surgery 21 (9) 793 -798 1993年09月 [査読無し][通常論文]
     
    EC/ICバイパス術施行例にpresaturation pulseを用いたMRAを施行した。MRAによりバイパスのpatencyが同定可能であるばかりではなく,MRAにpresaturation pulseを用いることにより血流の方向性が同定可能で,さらにはバイパスの機能評価に関しても有用な検査法になりうる
  • 脳血管障害へのアプローチ 脳ドック
    阿部 弘, 宝金 清博 内科 72 (3) 558 -562 1993年09月 [査読無し][通常論文]
  • 宝金 清博, 鎌田 恭輔, 阿部 弘 臨床放射線 38 (9) 997 -1005 1993年09月 [査読無し][通常論文]
  • K HOUKIN, K KAMADA, H KAMIYAMA, Y IWASAKI, H ABE, T KASHIWABA STROKE 24 (9) 1316 -1321 1993年09月 [査読無し][通常論文]
     
    Background and Purpose: Proton magnetic resonance spectroscopy has revealed changes in lactate and N-acetyl-aspartate in acute cerebral infarction. However, the details of these drastic changes and subsequent chronic changes have not been clarified. The purpose of this study was to disclose longitudinal changes in spectra seen in proton magnetic resonance spectroscopy. Methods: Six patients with completed cerebral infarction were examined longitudinally with localized proton magnetic resonance spectroscopy. Results: (1) In the acute stage (within 2 days after onset), two drastic changes were observed: N-acetyl-aspartate decreased rapidly and severely within 2 days after onset, and lactate increased immediately and reached a high level in the acute stage after onset. (2) In the chronic stage (more than 1 month after onset), two features were observed: lactate, which had increased in the acute stage, remained high for more than 1 month, and other signals such as those of N-acetyl-aspartate, choline, and phosphocreatine/creatine decreased dramatically. Conclusions: These results suggest that N-acetyl-aspartate and lactate as revealed by proton magnetic resonance spectroscopy can be useful indicators of the ischemic damage to the brain in clinical cases of cerebral infarction.
  • 宝金 清博, 鎌田 恭輔, 阿部 弘 臨床放射線 38 (9) p997 -1005 1993年09月 [査読無し][通常論文]
  • 一家族の同胞3人のすべてに発症を認めたモヤモヤ病の家族内発症例
    田中 徳彦, 上山 博康, 宝金 清博 小児の脳神経 18 (4) 329 -337 1993年08月 [査読無し][通常論文]
     
    本症の家族内発症例は文献上62組認められるが,同様の報告は過去に2例認めるのみであった。本症は多因子遺伝により規定される先天性要因の強い疾患であり,近年,HLA(Human Lymphocyte antigens)の検索がなされている。本症例においては,三兄弟に共通するHLA subtypeとして,A 24(9),BW 46,BW 61(40),CW 11を認めた。今回,家族内発症例に限定して,自験例を含み11組につきHLAの傾向を検討したが,一定の傾向はみられなかった
  • 宝金 清博, 鎌田 恭輔, 阿部 弘 Neurological Surgery 21 (8) 669 -675 1993年08月 [査読無し][通常論文]
  • K KAMADA, F TAKEUCHI, PJ KELLY, S KURIKI, O OSHIRO, DW ROBERTS, K HOUKIN, H ABE NEUROSURGERY 33 (2) 269 -273 1993年08月 [査読無し][通常論文]
     
    WE PRESENT A novel noninvasive technique for functional neurosurgical simulation combining three-dimensional magnetic resonance imaging of the brain surface with somatosensory evoked magnetic fields (SEFs) in a case of subcortical brain tumor. The preoperative analysis using this technique revealed that the central sulcus was located between the SEF source projected onto the brain surface and the tumor. At the time of surgery, the central sulcus was clearly identified by phase reversal of the cortical recordings of somatosensory evoked potentials. The actual cortical anatomy and the maximum somatosensory evoked potential location showed excellent agreement with the preoperative analysis of the brain surface magnetic resonance images and the SEF source. The subcortical tumor, which was barely identifiable without reliable functional cortical anatomy, was easily found in the motor area, just before the somatosensory evoked potential (and SEF) source. The potential clinical application of this technique is discussed.
  • 宝金 清博, 阿部 弘 INNERVISION 8 (6) 7 -10 1993年06月 [査読無し][通常論文]
  • S KURODA, H KAMIYAMA, H ABE, K HOUKIN, M ISOBE, K MITSUMORI, RC HEROS, JH WOOD, WR SELMAN NEUROSURGERY 32 (6) 912 -919 1993年06月 [査読無し][通常論文]
     
    IN A SERIES of 32 patients with internal carotid artery occlusion, regional cerebral blood flow (rCBF) and regional cerebral vasoreactivity (rCVR) were measured by xenon-133 single photon emission computed tomography and the acetazolamide test. We evaluated its usefulness in detecting the reduced cerebral perfusion reserve and predicting long-term prognosis. All Type 1 patients (normal rCBF and rCVR) were medically treated and experienced no recurrent ischemic attack. Cerebral hemodynamics remained unchanged. Type 2, 3, and 4 patients underwent superficial temporal artery-middle cerebral artery double anastomosis, if they consented to surgery. All Type 2 (normal rCBF and reduced rCVR) and Type 3 (reduced rCBF and rCVR) patients, who underwent surgery, showed no further ischemic attacks, as well as long-term normalization of rCVR, although long-term rCBF normalization was obtained in only three of seven Type 3 patients. Cerebral hemodynamics remained unchanged in Type 4 patients after surgery. In follow-up periods, major completed stroke occurred in all 3 Type 2 and Type 3 patients who were medically treated. These results suggest that the acetazolamide test is valuable in assessing the cerebral perfusion reserve and predicting long-term prognosis in patients with internal carotid artery occlusion, although further long-term or randomized studies are needed.
  • K KAMADA, O OSHIRO, F TAKEUCHI, S KURIKI, K HOUKIN, Y IWASAKI, H ABE JOURNAL OF THE NEUROLOGICAL SCIENCES 116 (1) 29 -33 1993年05月 [査読無し][通常論文]
     
    We present a novel non-invasive technique for identification of the central sulcus on the brain surface by using three-dimensional magnetic resonance imaging (3D-MRI) in combination with somatosensory evoked magnetic fields (SEFs). The central sulcus was supposed anatomically on the brain surface by using 3D-MRI. On the other hand, the primary somatosensory area is determined by using SEF data with median nerve stimulation. Superimposition of the SEFs of the 25 ms response onto the brain surface MR images clearly demonstrated the dipole source located in the gyral fold just behind the supposed central sulcus in all subjects analyzed. Three-dimensional reconstruction of the brain surface image data facilitated visualizing the precise anatomical localization of the magnetic field activities from any angle and measuring the distance from the source to any point of interest. The potential clinical application of this technique is discussed.
  • 青樹 毅, 松沢 等, 宝金 清博 Neurological Surgery 21 (4) 305 -311 1993年04月 [査読無し][通常論文]
     
    小児ウィリス動脈輪閉塞症20例についてMRI,MRA所見をX線血管撮影所見と比較検討した。1)3期,4期の基底核部モヤモヤ血管の描出はMRI・MRA両者とも全例可能であった。2)carotid fork等,主幹動脈の狭窄・閉塞性病変はMRIでは限局性の病変を除き十分に描出可能であった。MRAでは狭窄を過大評価する傾向が認められた。3)術後の頭蓋外からの血流の有無はMRAで十分診断可能であり,外来的な長期経過観察には特に有用と思われた。血流の方向性など詳細な情報は不十分と考えられた。4)3期,4期ウィリス動脈輪閉塞症はMRIおよびMRA各々で十分診断可能である。しかしモヤモヤ血管の描出,主幹動脈の狭窄性病変の描出は従来のX線血管撮影の分解能には未だ劣るために,現時点では"MRI・MRAのみ"での詳細な病期診断は不十分と思われた
  • Time-of-flight MR angiographyによる頭蓋内閉塞性血管病変の診断およびEC/ICバイパス術後の評価
    青樹 毅, 宝金 清博, 上山 博康 CI研究 15 (1) 21 -26 1993年02月 [査読無し][通常論文]
     
    脳血管の形態的検査法としては空間分解能の向上,機能的検査法としては血流の方向性の同定や流速の定量化,さらには他のMR情報との組合せなどにより多角的なMR診断が期待される
  • 神経系の新しい画像診断 3次元MRIとnavigation
    鎌田 恭輔, 宝金 清博, 阿部 弘 Clinical Neuroscience 11 (2) 159 -161 1993年02月 [査読無し][通常論文]
  • 鎌田 恭輔, 井須 豊彦, 宝金 清博 てんかんをめぐって 13 19 -27 1993年02月 [査読無し][通常論文]
  • 宝金 清博, 山上 博康, 阿部 弘, 小岩 光行, 柏葉 武, 野村 三起夫, 斎藤 久寿 新潟医学会雑誌 107 (1) 86 -86 1993年01月 [査読無し][通常論文]
  • 根本 正史, 青樹 毅, 宝金 清博, 小岩 光行, 相葉 武, 上山 博康, 阿部 弘 新潟医学会雑誌 107 (1) 89 -89 1993年01月 [査読無し][通常論文]
  • 鎌田 恭輔, 宝金 清博, 岩崎 喜信, 阿部 弘, 青樹 毅, 小岩 光行, 柏葉 武 新潟医学会雑誌 107 (1) 93 -93 1993年01月 [査読無し][通常論文]
  • 青樹 毅, 宝金 清博, 上山 博康 脳と神経 45 (1) 57 -62 1993年01月 [査読無し][通常論文]
     
    頭蓋内閉塞性血管病変に対するMRAの精度を評価するため,閉塞病変の診断能ならびに側副血行の描出能についてX線血管撮影所見との比較を中心に検討した。またEC/ICバイパス術後の血流の評価についても検討した。閉塞では,内頸動脈,中大脳動脈水平部(M1)の病変については十分MRAで同定可能であった。しかし,現時点での空間分離能では中大脳動脈第2分枝(M2)以降については未だ不十分であった。側副血行では,ウィリス動脈輪を介する血流は明瞭に描出されたが,leptomeningeal anastomosisの評価は困難であった。M1の狭窄病変での狭窄率と描出能との比較では,狭窄率が大きいほどMRA上狭窄が過大評価され,血流の乱れや流速の変化などが原因と思われた。EC/ICバイパス術後例では,バイパスのpatencyは十分に診断可能であり,さらにはバイパスの灌流状態をより生理的条件下で描出できる可能性を示唆した
  • CT, MRI時代の脳卒中学−新しい診断・治療大系−上巻
    日本臨床 51 125 -31 1993年 [査読無し][通常論文]
  • T. Aoki, K. Houkin, H. Kamiyama, Y. Iwasaki, H. Abe, M. Koiwa, S. Kawaguchi, T. Kashiwaba Neurological Surgery 21 (9) 793 -798 1993年 [査読無し][通常論文]
  • T. Aoki, K. Houkin, H. Kamiyama, Y. Iwasaki, H. Abe, M. Koiwa, S. Kawaguchi, T. Kashiwaba Brain and Nerve 45 (1) 57 -62 1993年 [査読無し][通常論文]
  • Satoshi Kuroda, Hiroyasu Kamiyama, Akihiro Takahashi, Kiyohiro Houkin, Hiroshi Abe, Hisatoshi Saitoh Neurologia Medico-Chirurgica 33 (12) 815 -819 1993年 [査読無し][通常論文]
     
    The accuracy of angioscopy in detecting atherosclerotic changes, such as plaque, ulcer, and mural thrombus, in the extracranial cerebral arteries was evaluated during carotid endarterectomy by comparison with angiographic and operative findings. Ten patients with internal carotid artery stenosis underwent intraoperative angioscopy during surgery. After clamping the carotid bifurcation, intraarterial atherosclerotic lesions were observed with an angioscope (0.8 or 1.4 mm outer diameter) inserted through a small incision in the common carotid artery. Angioscopic findings correlated well with both angiographic and operative findings in six patients, and provided additional information in two patients, such as organized thrombi within the ulcer and mural thrombi. Angioscopic findings were quite different to those from angiograms in two patients. In one, an ulcer on angiograms was false positive, and in the other, false negative. These findings were confirmed intraoperatively. Our results suggest that preoperative carotid angioscopy is of great value in detecting ulcers more accurately than angiography, and in selecting candidates for carotid endarterectomy, although further development of equipment is needed. © 1993, The Japan Neurosurgical Society. All rights reserved.
  • Satoshi Kuroda, Hiroyasu Kamiyama, Hiroshi Abe, Toru Yamauchi, Yoshihiko Kohama, Kiyohiro Houkin, Kenji Mitsumori Neurologia Medico-Chirurgica 33 (7) 434 -438 1993年 [査読無し][通常論文]
     
    Changes in the mean cerebral blood flow (mCBF) with growth in normal children (1-20 years old) were evaluated, and compared to serial mCBF changes in children with spontaneous occlusion of the circle of Willis (moyamoya disease) after vascular reconstructive surgery. mCBF gradually decreased with growth in control children. Preoperative mCBF was similar or decreased in moyamoya disease patients with transient ischemic attack, and slightly decreased in those with cerebral infarction. Postoperative mCBF gradually decreased similarly to physiological changes with growth in controls. These results suggest that changes with age should be considered when preoperative and long-term cerebral hemodynamics are evaluated in children with moyamoya disease. © 1993, The Japan Neurosurgical Society. All rights reserved.
  • [MR angiography in the diagnosis of intracranial vascular lesions]
    Nippon Rinsho. 51 125 -31 1993年 [査読無し][通常論文]
  • [Magnetic resonance spectroscopy in cerebral blood flow metabolism (series 8)]
    No Shinkei Geka. 21 669 -75 1993年 [査読無し][通常論文]
  • T. Aoki, H. Matsuzawa, K. Houkin, H. Kamiyama, H. Abe, K. Miyasaka, H. Saito Neurological Surgery 21 (4) 305 -311 1993年 [査読無し][通常論文]
  • Satoshi Kuroda, Hiroyasu Kamiyama, Hiroshi Abe, Toru Yamauchi, Yoshihiko Kohama, Kiyohiro Houkin, Kenji Mitsumori Neurologia Medico-Chirurgica 33 (7) 434 -438 1993年 [査読無し][通常論文]
     
    Changes in the mean cerebral blood flow (mCBF) with growth in normal children (1-20 years old) were evaluated, and compared to serial mCBF changes in children with spontaneous occlusion of the circle of Willis (moyamoya disease) after vascular reconstructive surgery. mCBF gradually decreased with growth in control children. Preoperative mCBF was similar or decreased in moyamoya disease patients with transient ischemic attack, and slightly decreased in those with cerebral infarction. Postoperative mCBF gradually decreased similarly to physiological changes with growth in controls. These results suggest that changes with age should be considered when preoperative and long-term cerebral hemodynamics are evaluated in children with moyamoya disease. © 1993, The Japan Neurosurgical Society. All rights reserved.
  • 川口 進, 鎌田 恭輔, 下山 三夫, 柏葉 武, 宝金 清博 日本てんかん学会プログラム・予稿集 0 (27) 137 -137 1993年 [査読無し][通常論文]
  • Satoshi Kuroda, Hiroyasu Kamiyama, Akihiro Takahashi, Kiyohiro Houkin, Hiroshi Abe, Hisatoshi Saitoh Neurologia Medico-Chirurgica 33 (12) 815 -819 1993年 [査読無し][通常論文]
     
    The accuracy of angioscopy in detecting atherosclerotic changes, such as plaque, ulcer, and mural thrombus, in the extracranial cerebral arteries was evaluated during carotid endarterectomy by comparison with angiographic and operative findings. Ten patients with internal carotid artery stenosis underwent intraoperative angioscopy during surgery. After clamping the carotid bifurcation, intraarterial atherosclerotic lesions were observed with an angioscope (0.8 or 1.4 mm outer diameter) inserted through a small incision in the common carotid artery. Angioscopic findings correlated well with both angiographic and operative findings in six patients, and provided additional information in two patients, such as organized thrombi within the ulcer and mural thrombi. Angioscopic findings were quite different to those from angiograms in two patients. In one, an ulcer on angiograms was false positive, and in the other, false negative. These findings were confirmed intraoperatively. Our results suggest that preoperative carotid angioscopy is of great value in detecting ulcers more accurately than angiography, and in selecting candidates for carotid endarterectomy, although further development of equipment is needed. © 1993, The Japan Neurosurgical Society. All rights reserved.
  • 脳血管障害に伴う浮腫脳の代謝 H-MRSによる検討
    宝金 清博 脳卒中 14 (7) 838 -839 1992年12月 [査読無し][通常論文]
  • 磁気共鳴spectroscopy(MRS)による完成梗塞(completed infarction)の検討
    宝金 清博 脳卒中 14 (7) 839 -839 1992年12月 [査読無し][通常論文]
  • Turbo-FLASH法を用いたMRI脳血流画像法 新たな脳循環測定法
    宝金 清博 脳卒中 14 (7) 866 -866 1992年12月 [査読無し][通常論文]
  • MRIを用いた脳循環血液量画像 超高速T1強調,T2強調画像法を用いて
    宝金 清博 脳循環代謝 4 (1) 106 -106 1992年11月 [査読無し][通常論文]
  • 宝金 清博, 上山 博康, 岩崎 喜信 Neurological Surgery 20 (11) 1155 -1160 1992年11月 [査読無し][通常論文]
     
    高速scan法(Torbo-FLASH)を用いて,dynamic MRIを行った.僅かな造影剤の投与により,良好なcontastが得られ,臨床的にも十分評価が可能な画像が得られる
  • 磁気共鳴画像法(MR)を用いた脳循環の評価 超高速T1強調,T2強調画像法を用いて
    宝金 清博 日本脳神経外科学会総会抄録集 51回 (2) 83 -83 1992年10月 [査読無し][通常論文]
  • 水素磁気共鳴spectroscopy (H-MRS)法による脳腫瘍診断と治療効果の判定
    宝金 清博 日本脳神経外科学会総会抄録集 51回 (特別講演・招待講演・シンポジウム等) 50 -50 1992年10月 [査読無し][通常論文]
  • 宝金 清博, 鎌田 恭輔, 青樹 毅 映像情報Medical 24 (21) 1203 -1208 1992年10月 [査読無し][通常論文]
  • 松沢 等, 宝金 清博, 野村 三起夫 Neurological Surgery 20 (10) 1049 -1054 1992年10月 [査読無し][通常論文]
     
    本法を施行した脳・脊髄血管疾患患者191例の内訳は,閉塞性血管疾患90例,モヤモヤ病16例,動静脈奇形39例,動脈瘤28例などであった.血管閉塞,動脈瘤,バイパス血管,及び動静脈奇形の異常血管を描出し得た.中大脳動脈の水平部分における狭窄所見を,本法と通常の血管造影とを比較した場合,32例の64血管における偽陽性率は39.1%であった.本法により患者の流体力学に関し臨床的に価値ある結果を得た.血管の解剖学的な像だけではなく,血流パターンに敏感な機能的な像をも示した
  • 脳梗塞のproton magnetic resonance spectroscopy (1H-MRS)
    宝金 清博, 鎌田 恭輔, 青樹 毅 日本磁気共鳴医学会雑誌 12 (4) 208 -214 1992年08月 [査読無し][通常論文]
     
    1)乳酸は,発症直後より急激に増加した.その後,数週間にわたって高値を持続し,数ヵ月後に,ゆるやかに組織より消退した.2) N-acetyl-aspartate (NAA)は,急性期,数日で徐々に減少し,慢性期もそのままであった.3)例外的症例を除いて,梗塞部位による差異は,認められなかった
  • 水素磁気共鳴spectroscopy (1HMRS)のroutine化とその問題点
    宝金 清博, 鎌田 恭輔, 松浦 元 日本磁気共鳴医学会雑誌 12 (1) 61 -70 1992年03月 [査読無し][通常論文]
  • 松沢 等, 飛騨 一利, 宝金 清博 脳と神経 44 (1) 24 -29 1992年01月 [査読無し][通常論文]
     
    空洞症におけるクモ膜下腔および空洞内髄液拍動現象について,pre-saturation pulseを用いて髄液を標識し,この移動をトレースする事で髄液拍動の定量的解析を試みた.Chiari奇形の空洞内には髄液拍動が著明に見られるが,外傷性空洞には拍動がほとんど見られず,Chiari奇形の空洞内髄液拍動パターンは,正常なクモ膜下腔拍動パターンとよく類似しているが,その拍動位相は症例によってまちまちであった.空洞内髄液拍動の原因としては,下垂した小脳扁桃ならびに脳幹下部が,直接脊髄実質を介して頭蓋内脈波を空洞内に伝播するか,大孔レベルのクモ膜下腔の髄液を介して頭蓋内脈波を空洞内に伝播するか,の2通りの伝播経路が推察された.空洞内の髄液と,脊髄クモ膜下腔の髄液の拍動の"位相差"が病態解析のためのポイントである
  • MRによる脳三次元画像の有用性と問題点
    CI研究 14 75 -83 1992年 [査読無し][通常論文]
  • SEFと3次元MRIによる脳外科手術navigationの試み.
    日本生体磁気学会誌 5 298 -301 1992年 [査読無し][通常論文]
  • I. Takahashi, T. Abumiya, H. Imamura, H. Saitoh, M. Nomura, K. Hokin, H. Abe Neurological Surgery 20 (2) 161 -164 1992年 [査読無し][通常論文]
  • H. Matsuzawa, K. Hida, K. Houkin, I. Yoshinobu, H. Abe, M. Akino, H. Saito Brain and Nerve 44 (1) 24 -29 1992年 [査読無し][通常論文]
  • K. Houkin, H. Kamiyama, Y. Iwasaki, H. Abe, K. Miyasaka, M. Koiwa, T. Kashiwaba Neurological Surgery 20 (11) 1155 -1160 1992年 [査読無し][通常論文]
  • H. Matsuzawa, K. Houkin, M. Nomura, H. Kamiyama, Y. Iwasaki, H. Abe, M. Akino, H. Saito Neurological Surgery 20 (10) 1049 -1054 1992年 [査読無し][通常論文]
  • K KAMADA, T ISU, K HOUKIN, T OHSATO, M KATOH, H KOJIMA NEUROSURGERY 29 (3) 464 -466 1991年09月 [査読無し][通常論文]
     
    The authors present a case of acute aggravation of subdural effusion associated with pachymeningitis carcinomatosa. Microscopic examination of a surgical specimen revealed diffuse involvement of the dura mater by a metastatic adenocarcinoma in which the tumor cells invaded venule located in the areolar layer in particular. The rapid increase in capillary transmural pressure resulted in extravasation of plasma components, causing an increase in subdural effusion.
  • 宝金 清博, 宮坂 和男, 田中 文基 脳と神経 43 (7) 665 -670 1991年07月 [査読無し][通常論文]
  • 宝金 清博, 松沢 等, 宮坂 和男 脳と神経 43 (6) 583 -587 1991年06月 [査読無し][通常論文]
     
    Stimulated Echo Acquisition Mode (STEAM)法による水素のspectroscopyを用い,脳梗塞症例の水素spectraの経時的変化を観察した.磁気共鳴画像やcomputer tomographyの異常所見が出現する前に,水素のspectraでは乳酸の急激な上昇とN-acetyl-aspartate (NAA)の減少などの劇的な変化が観察された.乳酸の上昇は24時間後も持続し,NAAの低下も持続した.NAAの低下は慢性期も見られ,乳酸の上昇も重症例では慢性期まで持続した
  • 刺創による脊椎損傷の1例.
    脳神経外科 19 255 -8 1991年 [査読無し][通常論文]
  • 脊椎, 脊髄腫瘍
    Karkinos 1 23 -29 1991年 [査読無し][通常論文]
  • I. Takahashi, T. Abumiya, H. Imamura, K. Houkin, H. Saitoh, Y. Iwasaki, T. Katoh, M. Nomura, M. Akino, T. Isu, H. Abe Neurological Surgery 19 (3) 255 -258 1991年 [査読無し][通常論文]
  • Houkin Kiyohiro, Saitoh Hisatoshi, Matsuzawa Hitoshi, Nomura Mikio, Kamiyama Hiroyasu, Iwasaki Yoshinobu, Abe Hiroshi Neurologia Medico-Chirurgica 31 (7) 385 -389 1991年 [査読無し][通常論文]
     
    Proton magnetic resonance spectroscopy (MRS) with the depth resolved surface coil spectroscopy technique and the 1331-2662 water suppression method was used to examine two cerebral ischemia patients and 10 normal volunteers. In all cases, N-acetyl-aspartate, creatine, phosphor creatine, and residual lipid were clearly observed. No lactic acid peak was observed in normal volunteers, but a large lactic acid peak appeared in the early stage of cerebral ischemia. This MRS abnormality was observed before abnormalities appeared in conventional imaging such as computed tomographic scanning and magnetic resonance imaging. © 1991, The Japan Neurosurgical Society. All rights reserved.
  • K. Houkin, H. Matsuzawa, K. Miyasaka, H. Kamiyama, Y. Iwasaki, H. Abe, K. Hida, M. Koiwa, T. Kashiwaba Brain and Nerve 43 (6) 583 -587 1991年 [査読無し][通常論文]
  • Houkin Kiyohiro, Saitoh Hisatoshi, Matsuzawa Hitoshi, Nomura Mikio, Kamiyama Hiroyasu, Iwasaki Yoshinobu, Abe Hiroshi Neurologia Medico-Chirurgica 31 (7) 385 -389 1991年 [査読無し][通常論文]
     
    Proton magnetic resonance spectroscopy (MRS) with the depth resolved surface coil spectroscopy technique and the 1331-2662 water suppression method was used to examine two cerebral ischemia patients and 10 normal volunteers. In all cases, N-acetyl-aspartate, creatine, phosphor creatine, and residual lipid were clearly observed. No lactic acid peak was observed in normal volunteers, but a large lactic acid peak appeared in the early stage of cerebral ischemia. This MRS abnormality was observed before abnormalities appeared in conventional imaging such as computed tomographic scanning and magnetic resonance imaging. © 1991, The Japan Neurosurgical Society. All rights reserved.
  • 癌診断におけるMRIの進歩 X線CTとの使い分け 脊椎・脊髄腫瘍
    宝金 清博, 宮坂 和男 KARKINOS 3 (12) 1259 -1265 1990年12月 [査読無し][通常論文]
  • 鎌田 恭輔, 井須 豊彦, 宝金 清博, 大里 孝夫, 加藤 正仁, 小浜 好彦, 小島 英明 新潟医学会雑誌 104 (12) 1062 -1062 1990年12月 [査読無し][通常論文]
  • 井須 豊彦, 鎌田 恭輔, 加藤 正仁, 山内 亨, 宝金 清博, 北岡 憲一, 小岩 光行, 阿部 弘 新潟医学会雑誌 104 (12) 1067 -1067 1990年12月 [査読無し][通常論文]
  • 鎌田 恭輔, 井須 豊彦, 宝金 清博, 大里 孝夫, 加藤 正仁, 小浜 好彦, 小柳 泉 新潟医学会雑誌 104 (12) 1067 -1068 1990年12月 [査読無し][通常論文]
  • 宝金 清博 北海道医学雑誌 65 (6) 595 -603 1990年11月 [査読無し][通常論文]
     
    ラットの局所脳梗塞モデルを用いて,リン31,及びH 1磁気共鳴spectroscopyにより高リン酸エネルギー代謝と乳酸の経時的変化を観察した.1)梗塞発生直後より梗塞部位において乳酸の急激な上昇が認められ,この高値は24時間後においても明瞭に認められた.2)これに対して,リン31の磁気共鳴spectroscopyでは梗塞発生直後phosphocreatineの減少と無機リン酸の増加が認められたが,24時間後にはほぼ正常パターンに復帰する傾向が認められた.3)これらの磁気共鳴spectroscopyの異常は病理学的変化や画像上の異常に先立って出現した.4)慢性期の梗塞部位においては,phosphomonoesterの高値が認められ,局所のgliosisを示していると考えられた
  • 脳動脈瘤手術におけるlaser doppler装置の有用性より安全確実なクリッピングのために
    宝金 清博 日本脳神経外科学会総会抄録集 49回 (1) 75 -75 1990年10月 [査読無し][通常論文]
  • K HOUKIN, IL KWEE, T NAKADA JOURNAL OF NEUROSURGERY 72 (5) 763 -766 1990年05月 [査読無し][通常論文]
  • 小島 英明, 斉藤 隆二, 久末 浩樹, 本田 千恵子, 大里 孝夫, 宝金 清博, 井須 豊彦, 竹林 克重, 山崎 知文 日本臨床細胞学会雑誌 29 (2) 200 -200 1990年03月22日 [査読無し][通常論文]
  • ミトコンドリアミオパチーの31P MR spectroscopyによる検討
    田代 邦雄, 伊藤 和則, 宝金 清博 厚生省精神・神経疾患研究委託費研究報告書 筋ジストロフィー症及び関連疾患の病態とその病因に関する研究 平成元年度 229 -233 1990年03月 [査読無し][通常論文]
     
    1) 44歳男,ミトコンドリアミオパチー症例にCoQを約1年間投与したが,臨床症状の改善と筋肉内での31P MR spectroscopyのPcr/Pi(運動負荷前後)をCoQ投与前後で比較すると臨床症状と平行してPcr/Piの低下と前値に回復するまでの時間の短縮が認められた.2) MELASの2症例に頭蓋内病巣部の31P MR spectroscopyを施行した.症例1は病巣側と正常側との差は認められなかった.症例2は病巣側のPcr/Piの低下が認められた
  • 野村 三起夫, 秋野 実, 宝金 清博 INNERVISION 5 (1) 14 -19 1990年01月 [査読無し][通常論文]
  • 上山 博康, 野村 三起夫, 阿部 弘, 鐙谷 武雄, 斉藤 久壽, 山内 享, 三森 研自, 吉本 哲之, 瀧川 修吾, 伊藤 輝史, 宝金 清博, 井須 豊彦, 河本 俊, 石川 達哉, 安井 信之 脳卒中の外科 18 (1) 50 -56 1990年 [査読無し][通常論文]
     
    The clinical, operative and pathological characteristics of intracranial dissecting aneurysms are presented. A review of the literature suggests that these types of intracranial aneurysms are being recognized with increasing frequency and can be characterized by its symp-tomatology and radiological patterns. For this five years, 31 cases with intracranial dissecting aneurysm were treated in our institute. The mean age and the site of aneurysm are assumed almost the same as that of previous reports, but the incidence of extravascular bleeding is 58% in our series, which may be higher than that of previous reports. Although the cause or mechanism of intracranial dissecting aneurysm has yet to be sufficient clarified and it largely depends upon future multilateral studies, but we would like to emphasize that the diagnosis is most important in this disease. It has been considered that"double lumen"on angiogram is a true diagnostic sign of dissecting aneurysm. Only four cases of 31 showed"double lumen", but no one showed it in acute stage. 17 cases (55%) showed"string and pearl"sign and 72% of the cases with ruptured dissecting aneurysm revealed it in acute stage. From these results, it seems to be quite all right to consider that"string and pearl"sign should be the true diagnostic sign in acute stage like as"double lumen"in chronic stage. We have obtained some interesting information through detailed examinations about surgical treatment of intracranial dissecting aneurysms. But we will mention it in detail on another occasion. Finally, we will conclude by listing the above-mentioned important points of the investigation about the di-agonosis of intracranial dissecting aneurysms.
  • K HOUKIN, T NAKADA, N SUZUKI, IL KWEE MAGNETIC RESONANCE IN MEDICINE 12 (3) 364 -368 1989年12月 [査読無し][通常論文]
  • T NAKADA, IL KWEE, N SUZUKI, K HOUKIN MAGNETIC RESONANCE IN MEDICINE 12 (2) 172 -180 1989年11月 [査読無し][通常論文]
  • 1H spectroscopyの臨床経験 脳梗塞症例および正常例の検討
    宝金 清博 日本脳神経外科学会総会抄録集 48回 347 -347 1989年09月 [査読無し][通常論文]
  • 実験脳梗塞急性期における1H spectroscopyと31P spectroscopyの経時的検討 乳酸蓄積の重要性
    宝金 清博 日本脳神経外科学会総会抄録集 48回 447 -447 1989年09月 [査読無し][通常論文]
  • 宝金 清博, 上野 一義, 多田 光宏 Neurologia medico-chirurgica 28 (12) 1163 -1169 1988年12月 [査読無し][通常論文]
     
    主幹動脈閉塞による脳梗塞39例を対象として,閉塞動脈の再開通群と非再開通群の比較検討を行った.1)再開通群では突発完成型の脳塞栓症が多く,転帰は非再開通群に比べ不良だった.2)再開通群では高率に出血性梗塞がみられたが,これは転帰を悪化させる因子ではない.3)再開通群において,急性期や亜急性期には血流量の有意な変化はみられなかったが,慢性期には低下した
  • 後頭蓋epidermoidと動脈の顔面神経同時圧迫による顔面痙攣の一例
    外科治療 57 468 -471 1988年 [査読無し][通常論文]
  • MRIによる脳幹梗塞の診断
    宝金 清博 臨床神経学 27 (6) 776 -776 1987年06月 [査読無し][通常論文]
  • 宝金 清博, 上野 一義, 多田 光宏 Neurologia medico-chirurgica 27 (4) 295 -301 1987年04月 [査読無し][通常論文]
     
    It is well-known that recanalization of the occluded arteries in a cerebral infarction is a common phenomenon observed in about 40% of cerebral infarctions caused by an occlusion of angiographically visible arteries. The precise process and clinical significance of the recanalization is, however, not always analyzed satisfactorily. The authors performed a follow-up angiography in 41 patients with a cerebral infarction which had an arterial occlusion in an initial angiography, and disclosed a recanalization of the occluded artery in 18 patients. The clinical and neuroradiological findings in the 18 patients are reviewed. Recanalization occurred within 7 days after a stroke. In well-documented cases, the recanalization seemed to occur around 4 days after the stroke. However, the precise process were not always uniform and there seemed to be several variations such as partial recanalization caused by migration of an embolus and incomplete recanalization due to gradual spontaneous clot lysis. In thrombotic occlusions the recanalization occurred later than in embolic occlusions, and, moreover, it had a tendency to result in incomplete recanalization which showed a residual stenosis in post-recanalized angiography. Computed tomography (CT) disclosed a hemorrhagic infarction in half of the recanalized cerebral infarctions and enhancement effects by an intravenously injected contrast medium were seen in almost all cases. These CT findings tended to appear later than the arterial recanalization itself. © 1987, The Japan Neurosurgical Society. All rights reserved.
  • Kazuyoshi Ueno, Izumi Koyanagi, Junichi Murata, Mitsuhiro Tada, Kiyohiro Houkin Neurol Med Chir 27 (2) 117 -121 1987年 [査読無し][通常論文]
     
    Three cases of acute subdural hematoma without head injury but attributable to surgically verified bleeding from a cortical artery are presented. Case 1: A 46-year-old male complained of a headache. Neurological examination revealed no abnormality. A subdural hematoma was found on computed tomography. Case 2: A 49-year-old male developed a consciousness disturbance. Removal of a hematoma was performed leaving mild confusion. Case 3: A 68-year-old female was admitted because of acute onset of confusion. An emergency operation was performed but profound hypotension occurred. Confusion persisted and the patient died from a complication. The previous reports on acute subdural hematoma caused by rupture of a cortical artery consisted of 17 cases with preceding trivial head trauma and 34 without. In most of the cases without head injury, the initial symptom was a headache mimicking that of subarachnoid hemorrhage which was followed by consciousness disturbance. Tearing of a cortical artery adhering to the dura mater by a trivial trauma or rapid head movement is proposed as a causative mechanism of the hematoma. In chronic subdural hematomas, only a negligible or no preceding head injury is often demonstrated. It is suggested, therefore, that a hemorrhage of the artery may cause an acute subdural hematoma if massive, and may also cause chronic subdural hematoma when it is minimal and subsequent bleeding from the hematoma membrane in the organizing process takes place. © 1987, The Japan Neurosurgical Society. All rights reserved.
  • Kiyohiro Houkin, Kazuyoshi Ueno, Mitsuhiro Tada, Hiroshi Ibara Neurologia Medico-Chirurgica 27 (4) 295 -301 1987年 [査読無し][通常論文]
     
    It is well-known that recanalization of the occluded arteries in a cerebral infarction is a common phenomenon observed in about 40% of cerebral infarctions caused by an occlusion of angiographically visible arteries. The precise process and clinical significance of the recanalization is, however, not always analyzed satisfactorily. The authors performed a follow-up angiography in 41 patients with a cerebral infarction which had an arterial occlusion in an initial angiography, and disclosed a recanalization of the occluded artery in 18 patients. The clinical and neuroradiological findings in the 18 patients are reviewed. Recanalization occurred within 7 days after a stroke. In well-documented cases, the recanalization seemed to occur around 4 days after the stroke. However, the precise process were not always uniform and there seemed to be several variations such as partial recanalization caused by migration of an embolus and incomplete recanalization due to gradual spontaneous clot lysis. In thrombotic occlusions the recanalization occurred later than in embolic occlusions, and, moreover, it had a tendency to result in incomplete recanalization which showed a residual stenosis in post-recanalized angiography. Computed tomography (CT) disclosed a hemorrhagic infarction in half of the recanalized cerebral infarctions and enhancement effects by an intravenously injected contrast medium were seen in almost all cases. These CT findings tended to appear later than the arterial recanalization itself. © 1987, The Japan Neurosurgical Society. All rights reserved.
  • 宝金 清博, 上野 一義, 多田 光宏, 井原 博 Neurologia medico-chirurgica 27 (4) 295 -301 1987年 [査読無し][通常論文]
     
    It is well-known that recanalization of the occluded arteries in a cerebral infarction is a common phenomenon observed in about 40% of cerebral infarctions caused by an occlusion of angiographically visible arteries. The precise process and clinical significance of the recanalization is, however, not always analyzed satisfactorily. The authors performed a follow-up angiography in 41 patients with a cerebral infarction which had an arterial occlusion in an initial angiography, and disclosed a recanalization of the occluded artery in 18 patients. The clinical and neuroradiological findings in...
  • 上野 一義, 小柳 泉, 村田 純一, 多田 光宏, 宝金 清博 Neurologia medico-chirurgica 27 (2) 117 -121 1987年 [査読無し][通常論文]
     
    Three cases of acute subdural hematoma without head injury but attributable to surgically verified bleeding from a cortical artery are presented. Case 1: A 46-year-old male complained of a headache. Neurological examination revealed no abnormality. A subdural hematoma was found on computed tomography. Case 2: A 49-year-old male developed a consciousness disturbance. Removal of a hematoma was performed leaving mild confusion. Case 3: A 68-year-old female was admitted because of acute onset of confusion. An emergency operation was performed but profound hypotension occurred. Confusion persist...
  • 脳梗塞急性期における動脈再開通例と非再開通例の比較検討
    宝金 清博 脳卒中 8 (6) 546 -547 1986年12月 [査読無し][通常論文]
  • 上野 一義, 井原 博, 多田 光宏, 宝金 清博 新潟医学会雑誌 100 (11) 693 -693 1986年11月
  • 宝金 清博 新潟医学会雑誌 100 (11) 694 -694 1986年11月 [査読無し][通常論文]
  • 宝金 清博, 宮坂 和男, 阿部 悟 Neurologia medico-chirurgica 26 (5) 392 -397 1986年05月 [査読無し][通常論文]
     
    Fracture of the articular process of a cervical vertebra has received little attention in the literature. This fracture is often overlooked because only a very subtle change is shown in conventional radiological examinations. In this review of 26 patients with cervical vertebra trauma examined with a high resolution computed tomography (CT) scanner (Somatom II) in the past 3 years, the authors found 6 cases with fracture of the articular process of a cervical vertebra. The diagnosis was mainly based on the very useful findings of CT scan. On the other hand, the plain neck films had less diagnostic value. Some indirect signs, however, such as an anterior subluxation and narrowing of the intervertebral foramen revealed in the plain neck films seemed valuable. The clinical symptoms of this fracture consisted of radicular injury and persistent neck pain. Hyperflexion injury, which seemed to be a notable tendency, was considered as a possible mechanism of this fracture in 2 cases. Anterior fusion was performed in 3 cases and in 3 other cases conservative therapy was continued. A therapeutic policy for this fracture has not been established. The authors emphasize that both anterior fusion and the removal of the fractured fragment are necessary when anterior subluxation is obvious and the fractured fragment has caused the radicular injury. © 1986, The Japan Neurosurgical Society. All rights reserved.
  • 脳塞栓症の検討 脳血管写所見による分離とその臨床的検討
    多田 光宏, 宝金 清博, 上野 一義 道南医学会誌 (21) 308 -312 1986年03月 [査読無し][通常論文]
  • 脳梗塞急性期における血管再開通の検討
    宝金 清博, 多田 光宏, 上野 一義 道南医学会誌 (21) 313 -316 1986年03月 [査読無し][通常論文]
  • 秋野 実, 鐙谷 武雄, 宝金 清博, 越前谷 幸平, 佐藤 正治 新潟医学会雑誌 100 (1) 41 -41 1986年01月
  • 宝金 清博 新潟医学会雑誌 100 (1) 56 -56 1986年01月 [査読無し][通常論文]
  • 甲状腺癌の頭蓋骨転移の一例
    医療 40 400 -403 1986年 [査読無し][通常論文]
  • Alpha-fetoprotein 産生胎児性癌に対して cis-platinum を含む多剤併用療法を行った1例
    小児の脳神経 11 215 -220 1986年 [査読無し][通常論文]
  • 脳虚血基礎と臨床
    1986年 [査読無し][通常論文]
  • Fracture of the Cervical Articular Processes - Clinical, Plain Radiographic and CT Evaluations.
    Neuro-Orthopedics 2 81 -86 1986年 [査読無し][通常論文]
  • 宝金 清博, 宮坂 和男, 阿部 悟, 藤谷 正紀, 岩崎 喜信, 阿部 弘 Neurologia medico-chirurgica 26 (5) 392 -397 1986年 [査読無し][通常論文]
     
    Fracture of the articular process of a cervical vertebra has received little attention in the literature. This fracture is often overlooked because only a very subtle change is shown in conventional radiological examinations.<BR> In this review of 26 patients with cervical vertebra trauma examined with a high resolution computed tomography (CT) scanner (Somatom II) in the past 3 years, the authors found 6 cases with fracture of the articular process of a cervical vertebra. The diagnosis was mainly based on the very useful findings of CT scan. On the other hand, the plain neck films had less...
  • 頸椎関節突起骨折の放射線学的診断
    宝金 清博 神経外傷 8 118 -118 1985年12月 [査読無し][通常論文]
  • 症候性てんかんを呈した脳動静脈奇形の検討 133Xeによる脳血流量測定
    宝金 清博 北海道医学雑誌 60 (4) 617 -617 1985年07月 [査読無し][通常論文]
  • 血友病Aに合併した脊髄硬膜外および硬膜下血腫の1例
    宝金 清博, 阿部 弘, 岩崎 喜信 小児の脳神経 10 (1) 1 -5 1985年02月 [査読無し][通常論文]
  • 症候性てんかんを示した脳動脈奇形の血流分布の検討.
    てんかんをめぐって いわゆる症候性てんかん 1985年 [査読無し][通常論文]
  • Reconstructive Surgery in 28 Cases of Moyamoya Disease Cerebral Revascularization for Stroke.
    Reconstructive Surgery in 28 Cases of Moyamoya Disease Cerebral Revascularization for Stroke. 1985年 [査読無し][通常論文]
  • 血友病Aに合併した脊髄硬膜外および硬膜下血腫の1例
    宝金 清博 小児の脳神経 9 (6) 539 -539 1984年12月 [査読無し][通常論文]
  • 宝金 清博 新潟医学会雑誌 98 (11) 556 -556 1984年11月 [査読無し][通常論文]
  • 宝金 清博, 佐藤 正治, 越前谷 幸平 Neurological Surgery 12 (3) 347 -352 1984年03月 [査読無し][通常論文]
  • 脊髄髄内腫瘍5例の検討 Syringomyeliaの合併例およびSub pial locationに関して
    宝金 清博 整形・災害外科 27 (2) 280 -280 1984年02月 [査読無し][通常論文]
  • 鞍結節髄膜腫
    阿部 弘, 徳田 耕一, 宝金 清博 日本脳神経外科コングレス講演録 3回 113 -128 1984年02月 [査読無し][通常論文]
  • Mixed pial-dural AVM の一例
    脳神経外科 12 347 -52 1984年 [査読無し][通常論文]
  • 虚血性脳血管障害の外科的治療−その現状、手術適応、手術術式並びに成績について−.
    北海道医学雑誌 59 515 -48 1984年 [査読無し][通常論文]
  • T. Aida, H. Abe, Y. Iwasaki, K. Hokin, M. Tsuru Neurological Surgery 12 (8) 943 -950 1984年 [査読無し][通常論文]
  • 宝金 清博 新潟医学会雑誌 97 (10) 483 -483 1983年10月 [査読無し][通常論文]
  • 宝金 清博, 阿部 弘, 岩崎 喜信 Neurologia medico-chirurgica 23 (5) 370 -374 1983年05月 [査読無し][通常論文]
     
    The patient, a 42-year-old female, was admitted with a 5-year history of progressive numbness of the bilateral lower limbs. Neurological examination disclosed left-sided weakness with hyperreflexia and hypesthesia under the Th<SUB>4</SUB> level. Metrizamide myelography showed a severe cord swelling from C<SUB>2</SUB> to C<SUB>7</SUB>. Metrizamide CT scan revealed a severe cord widening at the C<SUB>5, 6</SUB> level and displacement of the cord and the anterior median fissure to the right side at the C<SUB>6, 7</SUB> level. In addition, an exophytic extension to the left side at the C<SUB>7</SUB> level was recognized. Laminectomy was performed from C<SUB>2</SUB> through Th<SUB>1</SUB>. The tumor was located on the posterior surface of the spinal cord at the C<SUB>4-6</SUB> levels and covered by the pia mater, and the left posterior root penetrated the tumor. Another tumor was found at the C<SUB>7</SUB> level, in accordance with the pre-operative CT scan finding. The tumors were totally removed. Histopathological examination revealed a typical Antoni type A schwannoma. This case was a sub-pial schwannoma originating from the posterior root entry zone. Usage of the term "sub-pial" has been coined with the lipoma located under the pia mater. Nevertheless, the authors proposed that the present case should be classified as the spinal subpial tumor.
  • 血友病Aに起因する腰椎穿刺後の急性脊椎管内出血の1例
    宝金 清博 臨床神経学 23 (2) 184 -184 1983年02月 [査読無し][通常論文]
  • 宝金 清博, 阿部 弘, 会田 敏光 Neurological Surgery 11 (2) 217 -221 1983年02月 [査読無し][通常論文]
  • 頚椎黄靭帯石灰化腫瘤により脊髄症状を呈した1例
    脳神経外科 11 173 -8 1983年 [査読無し][通常論文]
  • 阿部弘 小児の脳神経 8 295 -304 1983年 [査読無し][通常論文]
  • Craniosynostosis の遠隔成績−機能的予後に影響を与える因子について−.
    臨床小児医学 31 313 -319 1983年 [査読無し][通常論文]
  • K. Hokin, H. Abe, T. Aida, S. Mabuchi, M. Tsuru, N. Nakamura Neurological Surgery 11 (2) 217 -221 1983年 [査読無し][通常論文]
  • M. Akino, H. Abe, Y. Iwasaki, K. Hokin, M. Tsuru, M. Koiwa, H. Saito Neurological Surgery 11 (2) 173 -178 1983年 [査読無し][通常論文]
  • 秋野 実, 宝金 清博, 伊古田 俊夫, 河本 俊, 馬淵 正二, 岩崎 喜信, 中川 翼, 阿部 弘, 北岡 憲一 日本てんかん学会プログラム・抄録集 0 (15) 68 -68 1981年09月19日 [査読無し][通常論文]
  • 乳児の第3脳室脈絡叢乳頭腫の1治験例
    宝金清博 臨床神経学 21 (4) 344 -344 1981年04月 [査読無し][通常論文]
  • 神経学的検査及び脳血管写上,髄外性病変の所見を呈した小児脳幹部神経膠腫の1例
    宝金清博 臨床神経学 20 (9) 771 -771 1980年09月 [査読無し][通常論文]
  • CTによるスクリーニングの落とし穴 頭部 検査の進め方
    INNERVISION 12 10 -13 1977年 [査読無し][通常論文]

書籍等出版物

所属学協会

  • 米国脳神経外科アカデミー   米国心臓病学会（AHA）   日本脳循環代謝学会   日本学術会議   米国脳神経外科学会   米国脳神経外科コングレス   国際磁気共鳴医学会   米国脳卒中学会   日本脳神経外科学会   日本脳卒中学会   The American Association of Neurological Surgeons   The Congress of Neurological Surgeons   The International Society for Magnetic Resonance in Medicine   The American Stroke Association   The Japan Neurosurgical Society   

共同研究・競争的資金等の研究課題

  • 日本学術振興会：科学研究費助成事業 基盤研究(B)
    研究期間 : 2019年04月 -2024年03月 
    代表者 : 伊東 雅基, 中山 若樹, 佐藤 典宏, 矢部 一郎, 数又 研, 浜内 祝嗣, 寳金 清博
     
    研究年度の3年目にあたる2021（R3）年度は、前年度末に完了した全対象者160名（もやもや病患者82名、健常比較対照者78名）の末梢血循環血漿から抽出精製した、血漿microRNAの発現量データの解析を行なった。すなわち、ハイスループットreal-time定量PCRアレイにより、関心microRNA17種類と内部および外部標準7種類（全24種）のmicroRNAのPCR測定値を、比較定量法により発現分析した。全160検体、24microRNAのうち、全被験者でCt値を検出可能であったのは、15microRNA（関心miRNA 8種類と内部および外部標準7種類）であったため、これら15microRNAの全発現量を用いてglobal normalizationによりdataの正規化をおこなった。続いて、15種の血漿microRNA発現profileを主成分分析により解析した結果、発現変動は第一主成分と第二主成分で二郡に分かれ、疾患群と比較対照群間で発現プロファイルに差があることが判明した。続いて、疾患群と対照群で関心microRNAの発現変動解析を実施した結果、3種類の関心microRNAで、二郡間での有意な発現変動を認めた。そのほかの関心microRNAについては、全体として発現量が極めて少ないためか、先行研究で認めた発現変動を確認できなかった。続いてmicroRNA発現量と、もやもや病の臨床的特徴との関連解析を開始した。その結果、microRNA発現量に関わらず、RNF213創始者変異（p.R4810K）を有する成人もやもや病患者では、直接・間接複合血行再建術後に、間接血行再建路が有意に発達することを見出した。間接血行再建路を介した血管新生反応には、microRNAによるエピゲノム制御ではなく、感受性遺伝子の遺伝的多型が関与していることが示唆された。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2019年04月 -2022年03月 
    代表者 : 鐙谷 武雄, 小松 晃之, 中山 若樹, 川堀 真人, 数又 研, 寳金 清博, 長内 俊也
     
    今回の研究では、中央大学で開発されたヘモグロビン(Hb) に複数のアルブミン(Alb) を結合させた形の人工酸素運搬体HemoActに、さらに抗酸化機能を付与するために、白金ナノ粒子(PtNP)、カタラーゼといった抗酸化物質をHemoActに結合あるいは共存させた2製剤を作製して、これらが脳虚血再灌流モデルにおいて脳保護効果検討することを目的としている。HemoAct中のHbは時間経過とともに自動酸化を生じてしまい、酸素運搬能が低下してしまうことが知られている。今回、HemoActに抗酸化能を付与することは酸素運搬能を維持させることが第一の目的であるが、さらにこの抗酸化能が虚血再灌流下の脳組織内で発生する活性酸素を除去する効果も期待している。今年度は作製する2製剤のうち、HemoActにPtNPを結合させたHemoAct-PtNPについて検討する予定で実験を開始した。 HemoAct-PtNPを調製するにあたり、当初Hb:PtNP = 1:1で結合したものを作ることを考えていたが、PtNPの水への溶解性が低いため、HemoActをかなり希釈してからPtNPと混合し、再度濃縮しなければならないことが判明した。従って調製法を再考する必要が発生し、結合比をHb:PtNP = 1:0.2くらいから調製することとなり、HemoAct-PtNP作製に時間を要した。現在のところパイロット実験用の試薬が届いたばかりの状態で、これから虚血再灌流モデルラットに投与して、その効果を検証する段階である。 この製剤調製に要した期間を利用して、新たに当教室の大学院生に虚血再灌流モデルラットの作製練習を行ってもらっており、安定した虚血再灌流モデルが作成できる段階に達してきている。これにより、オペレーターを複数用意できる状況になったため、やや遅れてしまった実験の進行を加速化できるものと考えている。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2019年04月 -2022年03月 
    代表者 : 中山 若樹, 伊東 雅基, 杉山 拓, 寳金 清博
     
    本研究は、脳動脈瘤の破裂機構を解明し、それを回避する薬剤治療を開拓することを目的として、 （1）ヒト臨床例の脳動脈瘤摘出標本、（2）ヒト臨床例の血管血流量測定および術前画像による流体力学的解析、（3）易破裂大型動脈瘤の新型動物実験モデル の『３本の柱』による包括的解析を行うものである。 破裂動脈瘤ヒト臨床例では、クリッピング後に、破裂点を温存しつつクリップの外側にある破裂点を含む動脈瘤壁を一塊として摘出した。破裂点の近傍では、壁在血栓や内皮下に数日前からのフィブリン析出や貪食細胞の習俗が見られている。 ヒト臨床例における動脈瘤の数値計算流体解析（computed flow dynamics; CFD）は先行研究によって壁せん断応力がむしろ低下した部位で破裂が起きている傾向がうかがえている。 ラット易破裂大型脳動脈瘤モデル動脈瘤標本による病理組織学的検討では、より安定したニトロ化合物を投与するために、給餌食混入ではなくニトロフマル酸化合物を腹腔内投与することとし、極量を安全に投与すべく、週に2回の投与を繰り返すことにした。これにより、嗅動脈分岐部の小型動脈瘤は9割の確率で誘導され、また約1割の個体では後大脳動脈近位部や後交通動脈に大型動脈瘤を誘導することができた。この結果は英文誌Journal of Neurosurgical Sciencesに採択が決まったところである。 ここでさらに大型動脈瘤の誘導率を高めるべく、閉経後の女性を模して雌ラットで卵管結紮も加えることにし、この条件で、薬剤投与群としてはアスピリン，シロスタゾールを、tail veinから静脈注射し、現在飼育中である。これらの動物モデルから得た標本において、内皮形態、壁在血栓、内皮出血、フィブリン析出、炎症細胞集簇の程度を、薬剤非投与群と比較して、そうした組織変化と破裂の関係性を評価していく予定である。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2018年04月 -2021年03月 
    代表者 : 穂刈 正昭, 川堀 真人, 数又 研, 寳金 清博
     
    本年度の進捗は以下のとおりである ①モヤモヤ病小児患者からの血液サンプル取得：モヤモヤ病小児患者35名から同意の元、血液サンプルを取得している。サンプルの白血球と血漿を分離後保存し北大病院Biobankにて保管している。これらを以降の実験に使用している。②ピロリ抗体、CagA抗体の検討：ELISA法（ピロリ：Eプレート“栄検”H.ピロリ抗体II、CagA：Human helicobactoer pyloricytotoxin-associated gene A protein IgGキット）で上記35名の感染既往を評価した。③遺伝子型の確認：上記35名および追加の2名においてRNF213の遺伝子多型を確認するためにDNA抽出、サンガー法によるGenotypingを行った。④研究期間中に手術治療を行った患者においては浅側頭動脈の採取を行った。 結果、35名中1名のみがピロリ抗体陽性で、他は陰性であった。ただこの患者はCagA抗体陰性であった。また1名ピロリ抗体陰性であったがCagA抗体陽性となった。遺伝子検索では、RNF213 正常GGが13名、変異型AGが23名、変異型AAは1名のみであった。STAはHE染色が終了し今後特殊染色に進む方針である これらの結果からモヤモヤ病発症にピロリ菌感染およびピロリ菌によるCagA産生が強く関わっているという仮説は否定される傾向が認められた。しかし今後も当初の研究計画に則り血漿内エクソソームの解析と浅側頭動脈の変性についての研究を進める。これらは今まで研究されていない分野で有り本研究が新たな病態解明に繋がると推測される。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2017年04月 -2020年03月 
    代表者 : 長内 俊也, 中山 若樹, 黒川 孝幸, 数又 研, 鐙谷 武雄, 寳金 清博
     
    X線視認性を得るためにタンタルパウダーとリピオドールをダブルネットワークゲルに取り込ませることに成功した。臨床で使用されている血管造影装置を使用し、透視確認実験を行なったところ、臨床で使用されている造影剤と遜色のない不透過性が得られた。従来型の塞栓物質は全くX線で視認されず、臨床使用に十分耐えうるX線下での視認性を誇ると考えられた。一方で、新たに開発した塞栓物質はカテーテル通過性の問題があるため、安定して定められた量の塞栓物質をラットへの投与ができるとは言いがたい状況であるが、少なくとも生体での安全性を確認するための実験は可能となった。 ラットの頸動脈からの経動脈投与のモデルに関しては、当初の注入量（1mL）では処置後に高率に痙攣を発症してしまうことが問題となった。検証すると塞栓物質の有無によらず、生理食塩水の投与でも同様な現象がおこるため、注入量を0.2mLにしたところ、安定したモデルの作成に成功した。正確な0.2mLの注入のためにロック付きの1mLのシリンジを使用したところ、投与が可能となった。現在従来のEmbosphereと塞栓物質を頚動脈から投与して、安全性を比較しているが、初期段階の結果では炎症反応はみられていない。 また、当施設で動物用のCTが撮影できることとなったため、頸動脈からの投与後に頭部CTを撮影してみたところタンタル入りのゲルはCTで高信号を示したのに対して、Embosphereは全く視認できなかった。このことから、実際に塞栓術を行なったのちにも頭部CTによって塞栓物質の分布が確認できる可能性があり、また従来の粒子状の塞栓物質では術後の体内動態を検証することが不可能であったため臨床的に有意義な結果であったと考えられた。 さらに別のX線視認性塞栓物質の候補としての金ナノ粒子をゲルに取り込ませることにより粒子の作成に成功した。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2016年04月 -2019年03月 
    代表者 : 鐙谷 武雄, 小松 晃之, 中山 若樹, 数又 研, 七戸 秀夫, 寳金 清博, 長内 俊也
     
    2時間脳虚血後に10℃に冷却した人工酸素運搬体HemoActを再開通動脈から投与した。この人工酸素運搬体投与＋冷却灌流のcombination therapy群を、10℃生理食塩水の同量投与、37℃HemoActの同量投与の二つmonotherapy群と、未治療コントロール群と比較して検討した。その結果、combination therapyの方が梗塞体積を縮小し、脳血流量低下を抑制する傾向にあった。この治療効果は、少なくとも治療7日後まで継続した。また、therapeutic time windowの延長効果の検討では、虚血時間が5時間までは治療効果があることが分かった。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2015年04月 -2018年03月 
    代表者 : 穂刈 正昭, 七戸 秀夫, 中山 若樹, 寳金 清博
     
    くも膜下出血急性期の炎症反応を制御し、神経損傷を軽減することが本研究の目的である。重症くも膜下出血モデルを作成し、それに対して低体温療法・ミノサイクリン投与を行うことによって、急性期炎症反応の制御および予後改善が可能であるか検討する。本研究を通じてくも膜下出血後の炎症反応に早期低体温療法は運動機能改善を伴う炎症反応の低下を認めたが、minocyclineは炎症反応の低下は認めるものの運動機能回復は認められないという結果であった。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2015年04月 -2018年03月 
    代表者 : 数又 研, 中山 若樹, 鐙谷 武雄, 七戸 秀夫, 寳金 清博
     
    iPS細胞より血管内皮細胞を分化誘導した。マイクロアレイを行った結果、もやもや病血管内皮細胞において、tight junction関連遺伝子であるCLDN1, Focal adhesions and hemidesmosomes関連遺伝子であるDST, NEXN, ITGB3が低下していた。RT-PCRでは、ITGB3はもやもや病血管内皮細胞において発現が低下していたが、DST, NEXNは有意な差はなく、CLDN1は明らかなバンドが確認できなかった。血管透過性アッセイにおいてY-27632を添加した際に、健常群では透過性が低下したのに対し、もやもや病においては亢進した。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2015年04月 -2018年03月 
    代表者 : 中山 若樹, 森脇 拓也, 穂刈 正昭, 鐙谷 武雄, 数又 研, 寳金 清博, 下田 祐介
     
    脳動脈瘤の破裂機構を解明するために、まずは易破裂性大型脳動脈瘤動物モデルの確立を目指した。雄SDラットに両側腎動脈後枝の結紮と高塩分食による高血圧を誘導し、左総頚動脈結紮によって血行力学的な負荷を与え、ニトロ化合物を安定なフマル酸化合物として腹腔内投与を1週間毎に行う方法を行った。投与量を様々に変更し、1400mg/kgにて約8割の個体に動脈瘤が発現し、22％には大型瘤が誘導された。 さらに大型瘤の誘導率を上げるべく、ヒト閉経後女性を模して雌ラットで卵巣を摘出し、加えて対側外頚動脈を結紮して血行力学的負荷を増強し、アスピリンやシロスタゾールによる薬剤投与実験を開始したところである。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2015年04月 -2018年03月 
    代表者 : 関 俊隆, 笹森 徹, 岩崎 素之, 七戸 秀夫, 寳金 清博
     
    脊髄癒着性くも膜炎は，くも膜・軟膜に慢性炎症性変化をきたし，慢性進行性に脊髄を障害する．確立された治療法はなく，きわめて治療困難である．難治性疼痛を認めることは稀ではなく，患者の日常生活を著しく損なう．本研究は脊髄損傷後の脊髄癒着性くも膜炎に起因する難治性疼痛の治療法の確立を目的とした．本実験の前に安定した脊髄損傷動物実験モデルを作製することとした．既存の動物脊髄損傷用の脳動脈瘤クリップは高価で継続的な実験が困難であったため，本邦の企業に依頼し動物脊髄損傷用の脳動脈瘤クリップを作製した．既存のクリップと比較して統計学的にも有意差のない脊髄損傷モデルを作製することできた．
  • 日本学術振興会：科学研究費助成事業 挑戦的萌芽研究
    研究期間 : 2015年04月 -2017年03月 
    代表者 : 寳金 清博, 中山 若樹, 数又 研, 鐙谷 武雄, 七戸 秀夫
     
    もやもや病の病態を解析するため、もやもや病特異的iPS細胞から平滑筋細胞を分化誘導した。まず、iPS細胞から神経堤細胞を分化誘導し、それらの細胞は99%以上でp75陽性であることを確認した。それらの細胞に対して平滑筋細胞へと分化誘導を行ったが、分化誘導の途中で細胞の老化、増殖能力の低下が認められた。最終的に残った細胞に対して免疫染色を行ったところ、ほぼ全ての細胞でα-SMA、SM22陽性であり、平滑筋細胞へと分化していることが示された。しかし、細胞の増殖能力低下のため、解析に必要な十分な細胞数を得ることが困難であった。今後、細胞の老化を防ぐために分化誘導方法を改良する必要があると考えられる。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2013年04月 -2016年03月 
    代表者 : 吉本 哲之, 中山 若樹, 数又 研, 七戸 秀夫, 寳金 清博, 佐々木 秀直, 伊東 雅基
     
    成人発症もやもや病の親から、小児もやもや病の子が生まれた場合（臨床的表現促進現象）、子はより顕著なもやもや病の遺伝的素因を保有している可能性がある。もやもや病親子例を対象として、全ゲノム網羅的マイクロアレイ遺伝子コピー数多型(CNV)解析を実施した。もやもや病親子15組中、表現促進を認めた10組では、16番染色体短腕上(16p13.3)の410kbpの領域にCNVを認める頻度が高く、遺伝子コピー数が、親世代より子世代で増幅している傾向を認めた。遺伝子コピー数の世代を超えた増幅が臨床的表現促進現象の背景となりうることが示唆され、次世代シーケンス解析による、より詳細な検証が必要である。
  • 日本学術振興会：科学研究費助成事業 挑戦的萌芽研究
    研究期間 : 2013年04月 -2015年03月 
    代表者 : 寳金 清博, 中山 若樹, 数又 研, 鐙谷 武雄, 七戸 秀夫
     
    本研究では、ウシBMECの過継代による老化モデル実験を行った。過継代P15～20 BMECにおいて巨細胞が出現し、β-galactosidase活性、活性酸素産生が増大していた。次いで、P5 BMECとP15 BMECで細胞の生死、アポトーシス、SIRT1発現を比較した。未刺激では差がなかったが、OGD/再酸素化の負荷刺激をすると、P15 BMECで細胞死、アポトーシスが増加した。一方、SIRT1発現は、P5 BMECでのみ負荷刺激後に増加した。この結果はSIRT1が細胞保護的に働いている可能性を示し、老化細胞でSIRT1の誘導がかかりにくいことが細胞の脆弱性に関連していることを想起させた。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2012年04月 -2015年03月 
    代表者 : 鐙谷 武雄, 宝金 清博, 中山 若樹
     
    本研究では、虚血再灌流モデルにおいて、経動脈的冷却灌流を行い、梗塞体積が1/3に縮小することを明らかにした。次いで、このモデルにおいて経時的な検討を行い、①AQP4での一過性発現亢進（2時間）、②アストロサイト終足の膨化と微小血管の圧迫狭窄（2～6時間）、③ICAM-1発現亢進（6～24時間）、④血管透過性亢進（6～24時間）、⑤梗塞の拡大（6～24時間）、⑥好中球浸潤、MMP9発現亢進、ミクログリアの増加（24時間）を認めた。治療群では、これらの現象がすべて抑制されており、冷却灌流による再灌流早期の低温化のインパクトが治療効果を発揮する上で重要であると考えられた。
  • 日本学術振興会：科学研究費助成事業 基盤研究(B)
    研究期間 : 2012年04月 -2015年03月 
    代表者 : 寳金 清博, 黒田 敏, 七戸 秀夫
     
    1) 患者血中EPCのFACS解析：患者33名、健康対照14名から採血を行った。術前では小児患者、成人患者ともにEPCの有意な細胞数低下が示された。また、術後は有意なCD34+/CD133+細胞の低下がみられた。これら所見は、病変部でのEPC消費亢進を示唆していると考えられた。 2) もやもや病患者由来iPS細胞の樹立：患者3名、正常対照3名からiPS細胞を作成した。血管内皮細胞への分化誘導を行い検討したところ、患者細胞では管腔形成能の低下がみられた。マイクロアレイとプロテオミクス解析では、患者で発現が低下している遺伝子/タンパク質に血管新生に関与するものが見出され、病因との関連が示唆された。
  • 日本学術振興会：科学研究費助成事業 基盤研究(B)
    研究期間 : 2011年04月 -2014年03月 
    代表者 : 七戸 秀夫, 寳金 清博, 黒田 敏
     
    ボランティア由来のBMSC(bone marrow stromal cells)を使用した。血小板濃厚液からPL(platelet lysate)を作成し、基本培地に加えてBMSCを培養した。PLで培養したBMSCは培養速度、栄養因子産生においてFCS(仔ウシ血清)で培養したものとほぼ同等であった。またPLで培養したBMSCは、ラット脳梗塞モデルへの移植後の運動機能回復においてもFCSで培養したものと同等以上であった。また小動物用MRIや18F-FDG PETで、ラット脳梗塞モデルへの移植後の細胞追跡や脳機能回復の画像化を試みた。これらの結果は、次世代の臨床試験プロトコールに取り入れられる。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2011年 -2013年 
    代表者 : 齊藤 正樹, 下濱 俊, 寳金 清博
     
    われわれは地域のケアスタッフが多様な医療知識を得る機会に恵まれていないことを明らかにした。脳卒中に関する知識は十分ではないが脳卒中に関する深い知識や急変時の初期対応、認知症に関する知識を得たいと考えていた。われわれは脳卒中と認知症ケアに関わる介護スタッフ向けの教育システムを開発した。特に赤平市においては脳卒中 TIA 救急処置に関する教育を行い、1年で市内の56％のスタッフに教育することに成功した。一連の研究データに基づき2冊の教育書も発刊した。
  • 日本学術振興会：科学研究費助成事業 挑戦的萌芽研究
    研究期間 : 2011年 -2012年 
    代表者 : 中山 若樹, 森脇 拓也, 寳金 清博, 黒田 敏
     
    これまで、臨床例における脳動脈瘤クリッピング術後の動脈瘤組織標本を計54個(破裂例40個、未破裂例14個)が取得できた。破裂瘤に特徴的な病理組織学的所見として、<1>内皮下のフィブリン析出、<2>内膜内へのリンパ球を主体とする炎症細胞の浸潤、<3>内壁表面の層状血栓形成などが見られた。一方で、免疫染色や走査電子顕微鏡による評価では、瘤壁にも機能は低下しながらも内皮細胞は存在しているようだが、破裂部近傍では形態や配列が乱れが顕著であったり、損失していたりすることが示唆された。これらの所見から鑑みると、動脈瘤は、壁せん断応力低下などの力学的要素に影響を受けた内皮障害に端を発し、その修復過程としての内壁の血栓形成あるいは壁内出血そしてそれに対する急激な炎症反応が、やがては壁の崩壊をきたすことで破裂すると推察される。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2009年 -2011年 
    代表者 : 飯星 智史, 寶金 清博
     
    今回私が行った研究は、現在脳動脈瘤治療の最先端治療としてカテーテルを使用した血管内治療に、骨髄幹細胞研究を合わせた、未来への治療の第1歩となる研究を行った。実臨床の血管内治療で使用するステントにこの骨髄幹細胞を生着させ、ミニブタ脳動脈瘤モデルにおいて治療効果の判定を行った。結果的には優位な効果を認めることはできなかったが、今後の未来への治療としてきわめて重要な1歩を踏み出したと考えている。
  • 日本学術振興会：科学研究費助成事業 挑戦的萌芽研究
    研究期間 : 2009年 -2011年 
    代表者 : 宮田 圭, 宝金 清博
     
    本研究は、ラット心肺停止モデルを用いて、全脳虚血に対する骨髄幹細胞による細胞治療の効果を検討したものである。 心肺停止-蘇生後脳症のモデル作成と評価方法を確立し、心肺停止後の-蘇生後脳の状態を、動物実験用MRIを用いて代謝(MRS機能で測定)や脳機能(fMRIで測定)を解析し、また、組織学的評価や行動学的評価する系を確立した。その上で、骨髄幹細胞の静脈内投与治療の治療効果を検討した結果、著明な治療効果が期待できることが判明した。 また、細胞治療の最適条件を投与細胞数や投与時期を変化させて検討を加えた結果、1x10^6個を可及的速やかに投与することが最適との結果が得られた。 さらに遺伝子組換え細胞を用いた実験により、BDNF, GDNF, PLGFなどの治療遺伝子を組み込んだ細胞は、脳虚血に対して更なる治療効果が得られる可能性があることが判明した。
  • 文部科学省：科学研究費補助金(基盤研究(B))
    研究期間 : 2008年 -2011年 
    代表者 : 本望 修, 寳金 清博, 濱田 洋文
     
    従来、"脳梗塞亜急性期における梗塞巣の損傷は完成してしまっており、既に不可逆的損傷を負ってしまっている、"と考えられていた梗塞巣が、実は、不可逆的変化に陥っていない脳組織が残存しており、治療の介入の余地が残されていることが推測されていた。本研究では、亜急性期の脳梗塞巣における病態生理を解析し、細胞治療による治療メカニズムを検討することで、新しい治療戦略を考案することを主目的としている。
  • 文部科学省：科学研究費補助金(基盤研究(C))
    研究期間 : 2008年 -2010年 
    代表者 : 齊藤 正樹, 寳金 清博, 米増 保之, 高橋 明, 高塚 伸太朗
     
    我々は過疎の進む地域において、脳卒中に関わる医療と福祉従事者(Stroke Care Worker)にstroke teamを中心に教育問題を調査し教育活動を行ってきた。多職種の受けた脳卒中教育の程度は様々であった。教育は量から質へ、卒前から卒後まで、院内から院外に対象を広げることが重要であると思われた。標準教材を開発することも重要である。
  • 文部科学省：科学研究費補助金(基盤研究(C))
    研究期間 : 2008年 -2010年 
    代表者 : 野中 雅, 本望 修, 寶金 清博, 本望 修
     
    脊髄損傷モデルラットに対して、骨髄幹細胞を静脈内に移植すると、著明な治療効果が認められることが、画像診断学的、組織学的、および行動学的に確認された。また、治療メカニズムには、BDNFを介した神経保護作用が強く関与しており、遺伝子組換え骨髄幹細胞を投与することで損傷局所のBDNF濃度を上げることで治療効果が更に高まることが判明した。また、神経再生作用も一部関与していることが判明した。さらに、これらの細胞治療による効果は、脊髄損傷発症後の超急性期に治療した場合のみならず、1か月以上経過した亜急性期に治療を行っても効果が認められたことより、"Therapeutic time window"は比較的広いことが判明した。
  • 文部科学省：科学研究費補助金(基盤研究(C))
    研究期間 : 2007年 -2009年 
    代表者 : 村上 友宏, 本望 修, 寳金 清博, 本望 修
     
    脊髄損傷モデルラットに対して、骨髄間葉系幹細胞を静脈内に移植すると、著明な治療効果が認められることが、画像診断学的、組織学的、および行動学的に確認された。また、治療メカニズムには、BDNFを介した神経保護作用が強く関与しており、また、神経再生作用も一部関与していることが判明した。さらに、これらの細胞治療による効果は、脊髄損傷発症後の超急性期に治療した場合のみならず、1か月以上経過した慢性期に治療を行っても効果が認められたことより、"Therapeutic time window"は比較的広いことが判明した。
  • 文部科学省：科学研究費補助金(基盤研究(C))
    研究期間 : 2007年 -2009年 
    代表者 : 小柳 泉, 本望 修, 寳金 清博, 秋山 幸功, 本望 修
     
    脊髄空洞症の成因に関して、実験的および臨床例での検討を行い、脊髄内の微小循環と組織間液の動態が重要な役割を果たしていることを確認した。脊髄空洞症では、組織間液の増大が空洞周囲の実質に存在し、中心管周囲から後索下部など、中央部から周囲へ拡大するように分布していた。これらの髄内浮腫は、治療による空洞の縮小とともに消失した。髄内の静脈系からの組織間液の吸収障害が、空洞形成の本態であると考えられた。
  • 文部科学省：科学研究費補助金(萌芽研究)
    研究期間 : 2006年 -2007年 
    代表者 : 寶金 清博, 石黒 雅敬, 石黒 雅敬
     
    セロトニンは平滑筋を直接的に収縮および内皮を介して拡張させる。血管収縮には5-HT_<2A>と、5-HT_<1B>、5-HT_<1D>受容体が関係しているが、血管部位や動物の種により大きな差異がある。脳においてセロトニンは神経から拡散し、血管平滑筋には血液脳関門を介さずに到達し、脳血管や冠血管の血管攣縮あるいはその持続に影響すると考えられている。多くのセロトニン受容体サブタイプとそれぞれの作動薬および拮抗薬が見出されているが、脳血管系におけるセロトニンの意義には未解決な点が多い。今回セロトニン非存在下とセロトニン存在下の両者での塩酸サルポグレラートの血管反応を調べることを目的とした。Wister rat(n=8)とNew Zealand white rabbit(n=11)の上小脳動脈および後大脳動脈から直径100-200μm、長さ3mmの血管を剥離し、Vessel Chamber(CH/2/A)に設定し、顕微鏡下で、Pressure Servo Control and Pump: Model PS/200/Q, FC(Living System Instrument, Burlington, VT, USA)を用いて、60mmHgの血管内圧をかけて、myogenic tone(rat12.9%±1.6,rabbit13.4%±3.1)を得た血管の血管径を測定した。セロトニン...
  • 文部科学省：科学研究費補助金(基盤研究(B))
    研究期間 : 2004年 -2007年 
    代表者 : 本望 修, 寶金 清博, 濱田 洋文
     
    ラット脳梗塞モデルに対して、遺伝子組換え骨髄幹細胞を静脈内に移植することにより、脳梗塞の治療を目的とした基礎的研究を遂行した。(1)ラット中大脳動脈閉塞モデルへの骨髄幹細胞移植における治療効果の機序の解明全身麻酔下に中大脳動脈を遮断することで、脳梗塞モデルとした。骨髄幹細胞を静脈内に移植した場合の治療効果の機序として、(1)局所のサイトカイン濃度が増大することによる神経保護作用、(2)血管新生の誘導、(3)アポトーシスの抑制効果、(4)抗浮腫効果、等が関係することが解明された。(2)ラット中大脳動脈閉塞モデルへの遺伝子組換え骨髄幹細胞の移植実験サイトカイン遺伝子(BDNF,GDNFなど)および血管新生遺伝子(VEGF,/Angiopoietin-1,PIGFなど)を骨髄幹細胞に導入し、移植治療の効果を検討した結果、治療メカニズムを増強する遺伝子を導入することで、更なる治療効果の向上が見込めることが判明した。(3)末梢血中の幹細胞の検討末梢血に存在する間葉系幹細胞の分離・培養・増殖する技術を確立し、脳梗塞ラットへの移植実験で、骨髄中に存在する間葉系幹細胞と同等の治療効果が期待できることを検証した。以上のように、補助金は補助条件に従って、非常に有効に使用されている
  • 文部科学省：科学研究費補助金(基盤研究(C))
    研究期間 : 2004年 -2006年 
    代表者 : 野中 雅, 本望 修, 寶金 清博
     
    当該研究計画では、骨髄幹細胞を利用することで、脊髄損傷に対する新治療法を現実化するための基礎的研究およびトランスレーショナルリサーチを目的とした。骨髄中には神経再生に有用な骨髄間葉系幹細胞(bone marrow derived mesenchymal stem cell : bMSC)が存在することは判明していたが、末梢血中に同様の幹細胞(peripheral blood derived mesenchymal stem cell : pMSC)が存在するか否かは不明であった。しかし、骨髄幹細胞を骨髄より取り出し末梢血中に移植すると、末梢血循環にのって脊髄に到達し、治療効果が発揮されることは判明していた。当該研究で、(1)末梢血中に存在する幹細胞の基礎解析、(2)末梢血中に存在する幹細胞の神経系細胞へのin vitroおよびin vivoでの分化、(3)骨髄に固定されている骨髄幹細胞の末梢血中への動員、を研究し、末梢血に存在する幹細胞が脊髄損傷の治療に有効である成果を得た。
  • 文部科学省：科学研究費補助金(萌芽研究)
    研究期間 : 2004年 -2005年 
    代表者 : 寶金 清博, 鹿野 恒, 小柳 泉, 本望 修, 三上 毅
     
    本研究では、心肺停止後の蘇生脳に起こる脳の変化を画像でとられ、その病理所見との比較を行うことで、予後診断へ役立てることを主目的としている。そこで、まず、高度脳虚血-再灌流モデルにおける、実験用高磁場MRIの検討を行った。また、臨床例における、心肺停止後の蘇生脳の画像、代謝を検討した。さらに、ラット心肺停止モデルにおいて、実験用高磁場MRIと早期病理診断(アポトーシス検出)との比較解析を行った。1.実験高度脳虚血-再灌流モデルのMRIラットの心肺停止後の蘇生後脳の状態をsimulationするモデルとして、ラットの中大脳動脈閉塞・再灌流モデルを用いて、高磁場(7テスラー)のMRI装置を用いて、画像撮像を行った。その結果、再灌流後、fractional anisotrophy image(FA画像)において、再灌流脳において異方性が早期に失われ、その後、そのanisotrophyを失った範囲において、梗塞の出現と慢性期において萎縮が進行することが示された。また、この部位において、magnetic resonance spectroscopy(MRS)を行うと、乳酸の出現とさらに正常状態では見られないlipidの出現が見られた。2.人における蘇生後脳症のMRI・MRS心肺停止後の蘇生後脳症で、予後不良例、良好例における、拡散強調画像、MRSの差異を検討した結果、予後良好例では、実験...
  • 文部科学省：科学研究費補助金(基盤研究(B))
    研究期間 : 2002年 -2004年 
    代表者 : 寶金 清博, 多田 光宏, 時野 隆至
     
    1、研究目的本研究では、本疾患に特異的な遺伝子異常や遺伝子発現の異常を探索した。以下の3つの視点から研究を行った。1)血液サンプリングによる家族発症もやもや病症例の遺伝子解析2)家族性もやもや病における表現促進現象とtriplet repeatの伸張の検討3)手術中に得られた患者の脳脊髄液のサイトカインの解析2、対象および方法1)家族性もやもや病関連遺伝子の探索(17q25の領域)以下の3つの手法で探索を行ったI)bioinformaticsの手法を用いた遺伝子探索II)既知遺伝子に対する変異解析III)SNPs解析2)家族性もやもや病における表現促進現象とtriplet repeatの伸張の検討もやもや病と141例を対象として17q25の領域のtriplet repeat伸張の有無を検討した。3)手術中に得られた患者の脳脊髄液のサイトカインの解析手術中に得られる脳表面の脳脊髄液のサイトカインの測定を行った。測定はELISA法で行った。3、結果1)遺伝子探索約250のUnigene Clusterをアミノ酸に翻訳して、ドメイン検索や他の機能タンパクとの相同性解析を行ったが、既知遺伝子の他には、明らかに原因遺伝子として適当なUnigene Clusterを検出することはできなかった。既知遺伝子に対する変異解析これまでに、DNAI2、AANAT、PSR、HCNGP、HN1、SGS...
  • 文部科学省：科学研究費補助金(基盤研究(B))
    研究期間 : 2002年 -2003年 
    代表者 : 本望 修, 濱田 洋文, 宝金 清博
     
    遺伝子組み換え骨髄幹細胞の脳神経領域における治療効果を、下記の方法で解析した。1.遺伝子組み換え細胞の作成(1)骨髄幹細胞にhTERT遺伝子を導入し、不死化した。(2)骨髄幹細胞にBDNF遺伝子を導入し、神経保護作用を増強させた。(3)骨髄幹細胞にIL-2遺伝子を導入し、抗腫瘍作用を増強させた。2.上記細胞の治療効果の確認(1)hTERT遺伝子を導入した不死化細胞のラット中大脳動脈閉塞モデルへの移植実験全身麻酔下に中大脳動脈を遮断することで、脳梗塞モデルとした。移植効果の検討は以下のごとく施行し、良好な結果が得られた。(A)移植幹細胞のホスト虚血脳内での生着・分化を組織学的に検討した結果、ドナー細胞はホスト組織内で良好な生着・増殖・分裂・分化・遊走・組織修復を示すことが判明した。(B)モーリス水迷路試験で記憶・学習能力・高次機能を解析した結果、移植により著名に症状が改善することが判明した。(C)トレッドミル運動負荷試験により運動機能の解析した結果、移植により顕著に運動機能が回復することが判明した。(D)動物実験用高磁場NMR装置1(7テスラー)を用い、同一個体の脳梗塞巣を経時的に画像診断学的に解析した結果、移植による治療効果は画像診断上も著名であることが判明した。(2)BDNF遺伝子を導入した細胞のラット中大脳動脈閉塞モデルへの移植実験上記脳梗塞へ移植し治療効果を判定したが、...
  • 文部科学省：科学研究費補助金(基盤研究(B))
    研究期間 : 1999年 -2001年 
    代表者 : 寶金 清博, 黒田 敏, 多田 光宏
     
    本研究計画は家族性モヤモヤ病が染色体17q25領域と連鎖するという我々が見いだした事実をもとに同領域に座位するモヤモヤ病原因遺伝子をクローニングすることを目的としている.1)本年度においては遺伝子変異を同定するための高速スクリーニング法である酵母universal stop codon assayを開発し,誌上発表を行った(Am J Pathol).2)現在,引き続き17q25に位置するD17S785からD17S836の9cMの領域に原因遺伝子を検索している.同領域には現在59の遺伝子が存在する(UCSC Human Genome Prject Working Draft http://genome.cse.ucsc.edu/index.html).3)現在までDNAI2,AANAT, PSR, HN1, HCNGPの遺伝子につきシーケンスを完了しているが,原因遺伝子の発見まで至っていない.研究期間終了後も候補遺伝子につき,機能的に可能性の高いものから順次シーケンスを行い原因遺伝子の同定を目指す予定である.
  • 文部科学省：科学研究費補助金(基盤研究(C))
    研究期間 : 1999年 -2001年 
    代表者 : 黒田 敏, 宝金 清博, 多田 光宏
     
    脳虚血におけるmPTの役割を詳細に検討する目的で、以下の実験を行なった。まず、げっ歯類では海馬CA1神経細胞が虚血に対してきわめて脆弱であるが、その機構にmPTが関与しているとの仮説を検証した。海馬と新皮質からミトコンドリアを単離し、カルシウム負荷によって生じるmPTを吸光度の変化として観察したが、両者のミトコンドリアのカルシウム感受性に明らかな差は認められなかった。そこで、カルシウム負荷にともなってミトコンドリアから放出されるcytochrome cをELISAを用いて定量的に測定したところ、海馬由来のミトコンドリアから有意に多量のcytoshrome cが放出されていることが判明した。その差がどこから生じるのかを明らかにするため、両者由来のミトコンドリアにおけるbcl-2関連蛋白の量をWestern blot法により解析した。その結果、海馬由来のミトコンドリアはbcl-XLの保有量が少ないことが判明した。この結果は現在、国際誌への投稿準備中である。以上の結果から、脳虚血による神経細胞死にはmPTそのものよりも、mPTにともなってミトコンドリアから放出される細胞死関連蛋白質やbcl-2関連蛋白が大きな役割を果たしていることが推測された。それをさらに検討する目的で、マウス中大脳動脈閉塞による脳梗塞モデルを確立し、calcineurin抑制剤であるFK506を投与した。その結果...
  • 文部科学省：科学研究費補助金(萌芽的研究)
    研究期間 : 1999年 -2000年 
    代表者 : 寶金 清博, 黒田 敏, 多田 光宏
     
    本研究の目的は膠芽腫細胞株においてアポトーシスに関与する種々の遺伝子異常を系統的に明らかにして,それら細胞株について低O_2耐性が各種腫瘍抑制遺伝子の状態に依存するものであるか,そしてそれがミトコンドリア透過性遷移とどう関係するのかをBcl-2ファミリー蛋白発現の状態と関連づけ明らかにすることである.本研究にて以下のような研究結果が得られている.1)膠芽腫細胞株においてp53,PTEN/MMAC1,p16,p14/ARFの状態を系統的に明らかにし,誌上発表を行った(Ishii, Tada, et al.Brain Pathol,1999).2)さらに膠芽腫におけるPTEN変異とΨPTEN偽遺伝子の発現を明らかにした(Zhang, Tada et al.Oncogne,2000)3)膠芽腫の一部ではEGFR増幅とPTEN変異によりAkt活性が極端に上昇しており,これがBadを介してapoptosisを強力に抑制していることが明らかになった.4)また一部ではp53変異によりp53依存性apotosis経路がブロックされていることが判明した.5)これら細胞株におけるミトコンドリア透過性遷移自体は種々の負荷に対して明らかには低下はしていないという結果を得た.すなわち,アポトーシスはミトコンドリアの上流経路でブロックされている.これら結果をまとめ近く誌上発表の予定である.
  • 文部科学省：科学研究費補助金(基盤研究(C))
    研究期間 : 1996年 -1998年 
    代表者 : 寳金 清博, 多田 光宏, 山内 亨
     
    ウィリス動脈輪閉塞症(モヤモヤ病)の家族内発症例を当施設で保有している6家系について,また九州大学脳神経外科学講座より提供を受けた24組の兄弟発生例につき血液白血球からDNA抽出を行い,モヤモヤ病との合併が報告されているneurofibromatosis typeI(von Recklinghausen disease)に注目し,neurofibromatosis typeI遺伝子すなわちNFl遺伝子が存在する第17番染色体について,家系内でのモヤモヤ病発症と17番染色体上の8つのmicrosatellite markerの連鎖を全自動シーケンサー上でのGeneScan analysis softwareにより解析した.この結果,17番染色体D17S939にてTwo point linkage analysisにてLOD score3.11を得て,連鎖の存在が確認された.またAffected Pedigree Member(APM)法においても同maker周辺計4個のmarkerについてp value<10-5を得た.1カ月以内に欧文誌上に発表すべく論文を投稿準備中である.さらに今後の予定としては,この領域から候補遺伝子を同定して,変異,発現の異常の有無を検討する.一方1家系は特殊な家系であり,モヤモヤ病発症と片頭痛発作が連鎖して発現しており,17番染色体には連鎖がいずれのm...
  • 文部科学省：科学研究費補助金(一般研究(C))
    研究期間 : 1993年 -1995年 
    代表者 : 今村 博幸, 宝金 清博, 寶金 清博, 宝金 清博, 寶金 清博, 宝金 清博, 澤村 豊, 多田 光宏
     
    本研究により,多くの成果が得られている.悪性神経膠腫(以下glioma)細胞がinterleukin-8 (IL-8)とくに77アミノ酸型のIL-8を産生することを見い出し,誌上発表を行っている(Tada M・他J Neuro-Oncol 15 : 25).また,tumor necrosis factor-α刺激によりglioma細胞がIL-6, IL-8, Mn-superoxide dismutase, IL-1などの種々の因子を産生することも見い出し,誌上発表を行った(Sawamura Y・他J Neuro-Oncol 15 : 197).さらにglioma細胞はgranulocyte-macrophagecolony-stimutating factor (Sawamura Y・他 Neuro Med Chir 33 : 603), monocyte chemoattractant protein-1 (Tada M・他Int J Cancer 58 : 240), interleukin-1 receptor antagonist (Tada M・J Neuroimmunol 50 : 187)を産生することを見い出しそれぞれ,誌上発表を行っている.こうしたglioma細胞が産生するサイトカインについては総説として論じている (Tada M・ 他J Neuro-On...
  • もやもや病の外科治療、遺伝子解析
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