研究者データベース

曽根 正光(ソネ マサミツ)
低温科学研究所 生物環境部門
助教

基本情報

所属

  • 低温科学研究所 生物環境部門

職名

  • 助教

J-Global ID

研究分野

  • ライフサイエンス / 分子生物学

職歴

  • 2020年01月 - 現在 北海道大学 低温科学研究所 生物環境部門 助教
  • 2017年07月 - 現在 千葉大学 大学院医学研究院 特任助教
  • 2010年05月 - 2017年06月 京都大学 iPS細胞研究所 特定研究員
  • 2008年04月 - 2010年04月 理化学研究所 中川独立主幹研究ユニット 特別研究員

学歴

  • 2003年04月 - 2008年03月   京都大学   生命科学研究科

研究活動情報

論文

  • Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas
    Shingo Komura, Kenji Ito, Sho Ohta, Tomoyo Ukai, Mio Kabata, Fumiaki Itakura, Katsunori Semi, Yutaka Matsuda, Kyoichi Hashimoto, Hirofumi Shibata, Masamitsu Sone, Norihide Jo, Kazuya Sekiguchi, Takatoshi Ohno, Haruhiko Akiyama, Katsuji Shimizu, Knut Woltjen, Manabu Ozawa, Junya Toguchida, Takuya Yamamoto, Yasuhiro Yamada
    Nature Communications 10 3999  2019年09月 [査読有り][通常論文]
  • 曽根 正光, 山本 拓也
    生化学 91 1 7 - 16 (公社)日本生化学会 2019年02月 [査読有り][通常論文]
     
    多能性幹細胞は,がん細胞にも比類する旺盛な増殖能力を持ち,好気的解糖がそれを支えている.そのため,ミトコンドリアの酸化的リン酸化を主体としてエネルギー代謝を行う体細胞がiPS細胞へと初期化されるときには,解糖系やグルタミン代謝の活性化,ミトコンドリアの断片化など,さまざまな代謝系の変化が引き起こされる.こうした代謝様式のシフトは細胞増殖を亢進するだけではなく,エピゲノム変化に重要な役割を果たすことが明らかになってきた.また,多能性幹細胞にはナイーブ型とプライムド型と呼ばれる未分化段階の異なる二つの状態が存在し,それらの間でも代謝様式に興味深い違いがある.本稿では,分化多能性の維持と獲得に代謝がどのように関与するのかについて,近年の知見を踏まえて概観する.(著者抄録)
  • Hiroki Ikeda, Masamitsu Sone, Shinya Yamanaka, Takuya Yamamoto
    NATURE COMMUNICATIONS 8 1 1616  2017年11月 [査読有り][通常論文]
     
    Higher-order chromatin organization controls transcriptional programs that govern cell properties and functions. In order for pluripotent stem cells (PSCs) to appropriately respond to differentiation signals, developmental gene loci should be structurally and spatially regulated to be readily available for immediate transcription, even though these genes are hardly expressed in PSCs. Here, we show that both chromatin interaction profiles and nuclear positions at developmental gene loci differ between human somatic cells and hPSCs, and that changes in the chromatin interactions are closely related to the nuclear repositioning. Moreover, we also demonstrate that developmental gene loci, which have bivalent histone modifications, tend to colocalize in PSCs. Furthermore, this colocalization requires PRC1, PRC2, and TrxG complexes, which are essential regulatory factors for the maintenance of transcriptionally poised developmental genes. Our results indicate that higher-order chromatin regulation may be an integral part of the differentiation capacity that defines pluripotency.
  • Masamitsu Sone, Nobuhiro Morone, Tomonori Nakamura, Akito Tanaka, Keisuke Okita, Knut Woltjen, Masato Nakagawa, John E. Heuser, Yasuhiro Yamada, Shinya Yamanaka, Takuya Yamamoto
    Cell Metabolism 25 5 1103 - 1117.e6 2017年05月 [査読有り][通常論文]
  • Joanna Y. Ip, Masamitsu Sone, Chieko Nashiki, Qun Pan, Kiyoyuki Kitaichi, Kaori Yanaka, Takaya Abe, Keizo Takao, Tsuyoshi Miyakawa, Benjamin J. Blencowe, Shinichi Nakagawa
    SCIENTIFIC REPORTS 6 1 27204  2016年06月 [査読有り][通常論文]
     
    The long noncoding RNA Gomafu/MIAT/Rncr2 is thought to function in retinal cell specification, stem cell differentiation and the control of alternative splicing. To further investigate physiological functions of Gomafu, we created mouse knockout (KO) model that completely lacks the Gomafu gene. The KO mice did not exhibit any developmental deficits. However, behavioral tests revealed that the KO mice are hyperactive. This hyperactive behavior was enhanced when the KO mice were treated with the psychostimulant methamphetamine, which was associated with an increase in dopamine release in the nucleus accumbens. RNA sequencing analyses identified a small number of genes affected by the deficiency of Gomafu, a subset of which are known to have important neurobiological functions. These observations suggest that Gomafu modifies mouse behavior thorough a mild modulation of gene expression and/or alternative splicing of target genes.
  • Joonseong Lee, May Nakajima-Koyama, Masamitsu Sone, Makito Koga, Miki Ebisuya, Takuya Yamamoto, Eisuke Nishida
    STEM CELL REPORTS 5 4 480 - 489 2015年10月 [査読有り][通常論文]
     
    The role of secreted molecules in cellular reprogramming has been poorly understood. Here we identify a truncated form of ephrin receptor A7(EPHA7) as a key regulator of reprogramming. Truncated EPHA7 is prominently upregulated and secreted during reprogramming. EPHA7 expression is directly regulated by OCT3/4. EphA7 knockdown results in marked reduction of reprogramming efficiency, and the addition of truncated EPHA7 is able to restore it. ERK activity is markedly reduced during reprogramming, and the secreted, truncated EPHA7 is responsible for ERK activity reduction. Remarkably, treatment of EphA7-knockdown MEFs with the ERK pathway inhibitor restores reprogramming efficiency. Analyses show that truncated EPHA7-induced ERK activity reduction plays an important role in the middle phase of reprogramming. Thus, our findings uncover the importance of secreted EPHA7-induced ERK activity reduction in reprogramming.
  • Koichiro Homma, Masakatsu Sone, Daisuke Taura, Kenichi Yamahara, Yutaka Suzuki, Kazutoshi Takahashi, Takuhiro Sonoyama, Megumi Inuzuka, Yasutomo Fukunaga, Naohisa Tamura, Hiroshi Itoh, Shinya Yamanaka, Kazuwa Nakao
    ATHEROSCLEROSIS 212 1 42 - 47 2010年09月 [査読有り][通常論文]
     
    Objective: We previously succeeded in inducing and isolating vascular endothelial cells (ECs) from both human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Here, we compared the functionality of human adult aortic ECs (HAECs), human ES-derived ECs (ESECs) and human iPS-derived ECs (iPSECs). Methods and results: We compared the cell proliferative potential, potential for migration, and tolerance to oxidative stress. ESECs were significantly superior to HAECs in all of these cell functions. The cell functions of iPSECs were comparable to those of ESECSs and also superior to HAECs. We then analyzed the gene expressions of HAECs, ESECs and iPSECs, and observed that the expression level of Sirt1, a nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase, is higher in ESECs and iPSECs than in HAECs. The inhibition of Sirt1 with a Sirt1-specific inhibitor and siRNA antagonized these differences between the three types of cells. Conclusions: Sirt1 plays a key role in the high cellular function of ESECs and iPSECs. Although further in vivo investigations are required, this study initially demonstrated the potential of ESECs and iPSECs as the cell source for regenerative medicine, and also showed the potential of ES cells as a useful tool for elucidating the molecular mechanism of cell aging. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
  • Daisuke Taura, Masakatsu Sone, Koichiro Homma, Naofumi Oyamada, Kazutoshi Takahashi, Naohisa Tamura, Shinya Yamanaka, Kazuwa Nakao
    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 29 7 1100 - 1103 2009年07月 [査読有り][通常論文]
     
    Objective-Induced pluripotent stem (iPS) cells are a novel stem cell population derived from human adult somatic cells through reprogramming using a defined set of transcription factors. Our aim was to determine the features of the directed differentiation of human iPS cells into vascular endothelial cells (ECs) and mural cells (MCs), and to compare that process with human embryonic stem (hES) cells. Methods and Results-We previously established a system for differentiating hES cells into vascular cells. We applied this system to human iPS cells and examined their directed differentiation. After differentiation, TRA1-60(-) Flk1(+) cells emerged and divided into VE-cadherin-positive and-negative populations. The former were also positive for CD34, CD31, and eNOS and were consistent with ECs. The latter differentiated into MCs, which expressed smooth muscle alpha-actin and calponin after further differentiation. The efficiency of the differentiation was comparable to that of human ES cells. Conclusions-We succeeded in inducing and isolating human vascular cells from iPS cells and indicate that the properties of human iPS cell differentiation into vascular cells are nearly identical to those of hES cells. This work will contribute to our understanding of human vascular differentiation/development and to the development of vascular regenerative medicine. (Arterioscler Thromb Vasc Biol. 2009; 29: 1100-1103.)
  • Daisuke Taura, Michio Noguchi, Masakatsu Sone, Kiminori Hosoda, Eisaku Mori, Yohei Okada, Kazutoshi Takahashi, Koichiro Homma, Naofumi Oyamada, Megumi Inuzuka, Takuhiro Sonoyama, Ken Ebihara, Naohisa Tamura, Hiroshi Itoh, Hirofumi Suemori, Norio Nakatsuji, Hideyuki Okano, Shinya Yamanaka, Kazuwa Nakao
    FEBS LETTERS 583 6 1029 - 1033 2009年03月 [査読有り][通常論文]
     
    Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-L-ornithine and fibronectin-coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBP alpha, PPAR gamma 2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
  • Masamitsu Sone, Tetsutaro Hayashi, Hiroshi Tarui, Kiyokazu Agata, Masatoshi Takeichi, Shinichi Nakagawa
    JOURNAL OF CELL SCIENCE 120 15 2498 - 2506 2007年08月 [査読有り][通常論文]
     
    Recent transcriptome analyses have revealed that a large body of noncoding regions of mammalian genomes are actually transcribed into RNAs. Our understanding of the molecular features of these noncoding RNAs is far from complete. We have identified a novel mRNA-like noncoding gene, named Gomafu, which is expressed in a distinct set of neurons in the mouse nervous system. Interestingly, spliced mature Gomafu RNA is localized to the nucleus despite its mRNA-like characteristics, which usually act as potent export signals to the cytoplasm. Within the nucleus, Gomafu RNA is detected as numerous spots that do not colocalize with known nuclear domain markers. Gomafu RNA is extremely insoluble and remains intact after nuclear matrix preparation. Furthermore, heterokaryon assays revealed that Gomafu RNA does not shuttle between the nucleus and cytoplasm, but is retained in the nucleus after its transcription. We propose that Gomafu RNA represents a novel family of mRNA-like noncoding RNA that constitutes a cell-type-specific component of the nuclear matrix.

その他活動・業績

  • Hybrid cellular metabolism coordinated by Zic3 and Esrrb synergistically enhances somatic cell reprogramming.
    Masamitsu Sone, Shinya Yamanaka, Takuya Yamamoto ISSCR 2017, Boston, USA, June 14-17, 2017 2017年06月 [査読有り][通常論文]
  • ORPHAN NUCLEAR RECEPTOR AND ZIC FAMILY TRANSCRPTION FACTORS SYNERGISTICALLY ENHANCE SOMATIC CELL REPROGRAMMING
    Sone M, Yamanaka S, Yamamoto T 第13 回ISSCR(2015.6.24-27 ストックホルム) 2015年06月 [査読有り][通常論文]
  • CHROMATIN INTERACTIONS AT DEVELOPMENTAL GENE LOCI ARE REESTABLISHED BY SOMATIC CELL REPROGRAMMING
    Hiroki Ikeda, Masamitsu Sone, Shinya Yamanaka, Takuya Yamamoto 第13 回ISSCR(2015.6.24-27 ストックホルム) 2015年06月 [査読有り][通常論文]
  • ANALYSIS OF LONG-RANGE CHROMATIN INTERACTION NETWORKS IN PLURIPOTENT STEM CELLS
    Hiroki Ikeda, Masamitsu Sone, Shinya Yamanaka, Takuya Yamamoto 第12 回ISSCR(2014.6.18-21 バンクーバー) 2014年06月 [査読有り][通常論文]
  • Inferring the transcription networks required for induction of pluripotecy
    Sone M, Yamanaka S, Yamamoto T 第10 回ISSCR(2012.6.13-16.横浜) 2013年06月 [査読有り][通常論文]
  • Exploration of chromatin interactions in pluripotent stem cells
    Hiroki Ikeda, Masamitsu Sone, Shinya Yamanaka, Takuya Yamamoto 第11 回ISSCR(2013.6.12-15 ボストン) 2013年06月 [査読有り][通常論文]
  • Global analysis of higher-order chromatin structure in pluripotent stem cells
    Ikeda H, Sone M, Yamanaka S, Yamamoto T 第10 回ISSCR(2012.6.13-16.横浜) 2012年06月 [査読無し][通常論文]
  • 曽根正光, 築地仁美, 芳本玲, 吉田稔, 北市清幸, 山本経之, 阿部高也, 高雄啓三, 宮川剛, 中川真一 日本RNA学会年会要旨集 12th 70 2010年07月27日 [査読無し][通常論文]
  • 曽根正光, 築地仁美, 甲斐田大輔, 吉田稔, 中川真一 日本RNA学会年会要旨集 10th 4 2008年07月23日 [査読無し][通常論文]
  • 築地仁美, 曽根正光, 中川真一 日本RNA学会年会要旨集 9th 234 2007年07月28日 [査読無し][通常論文]
  • 曽根正光, 稲垣智子, 竹市雅俊, 中川真一 日本RNA学会年会要旨集 9th 231 2007年07月28日 [査読無し][通常論文]
  • 曽根正光, 竹市雅俊, 中川真一 日本RNA学会年会要旨集 8th 37 2006年07月18日 [査読無し][通常論文]
  • 曽根正光, 竹市雅俊, 中川真一 日本分子生物学会年会講演要旨集 28th 74 2005年11月25日 [査読無し][通常論文]
  • 曽根正光, 竹市雅俊, 中川真一 日本発生生物学会大会発表要旨集 37th 77 2004年05月10日 [査読無し][通常論文]

受賞

  • 2017年 International Society for Stem Cell Research Merit Award
     
    受賞者: 曽根 正光
  • 2007年 The Company of Biologists JCS Prize
     
    受賞者: 曽根 正光


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