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GOWDA　DIVYAVANI (ゴウダ　デヴイヤヴアアニ)

保健科学研究院助教

研究者基本情報

■ URL
researchmap URLホームページURL■ ID 各種
研究者番号
  • 40875952
ORCID IDJ-Global ID■ 研究キーワード・分野
研究分野
  • 環境・農学, 化学物質影響, Organic aerosols
■ 担当教育組織

経歴

■ 経歴
学歴
  • 2020年03月 - 現在, Assistant professor, Department of Health Science, Hokkaido University.
  • 2019年09月 - 2020年02月, Academic Research at Department of Health Science, Hokkaido University.
  • 2017年04月 - 2019年05月, Technical assistant at Center for Integrative Medical sciences, Riken Yokohama.
  • 2016年10月 - 2017年02月, Postdoctoral fellow at Institute of Low Temperature Science, Hokkaido University, Postdoctoral fellow
  • 2011年07月 - 2012年02月, Lecture at Bharathi Education trust
  • 2009年09月 - 2011年06月, Master student, BET, University of Mysore.

研究活動情報

■ 論文
  • Development of analytical methods for the quantification and enhanced detection of unsaturated short-chain FAHFAs
    Rachana M. Gangadhara; Perumalsamy Parasuraman; Divyavani Gowda; Hariom Yadav; Ganesh V. Halade; Shu-Ping Hui; Siddabasave Gowda B. Gowda
    Analytica Chimica Acta, 1404, 345435, 345435, Elsevier BV, 2026年06月
    研究論文（学術雑誌）
  • Comprehensive lipidomic profiling of dietary Indian millets and identification of fatty acid esters of hydroxy fatty acids by untargeted LC/MS
    Kumari Suraksha; Divyavani Gowda; Chandra Nayaka Siddaiah; Tara Satyavathi Chellapilla; Shu-Ping Hui; Siddabasave Gowda B. Gowda
    Food Chemistry, 511, 148792, 148792, Elsevier BV, 2026年05月
    研究論文（学術雑誌）
  • Lipidomic signatures reveal biomarkers of mild cognitive impairment
    Jayashankar Jayaprakash; Siddabasave Gowda B. Gowda; Divyavani Gowda; Hariom Yadav; Shalini Jain; Amanda Smith; Ambuj Kumar; Corinne Labyak; Michal Masternak; Peter J. Holland; Marc E. Agronin; Andrea Y. Arikawa; Cynthia White-Williams; Shalini Jain; Hariom Yadav; Shu-Ping Hui
    Translational Psychiatry, 16, 1, Springer Science and Business Media LLC, 2026年02月16日
    研究論文（学術雑誌）
  • Brain lipidomics for region-specific biomarker discovery in neurodegenerative diseases
    Jayashankar Jayaprakash; Solomon Tebeje Gizaw; Divyavani Gowda; Hiroshi Hinou; Shin-Ichiro Nishimura; Shu-Ping Hui; Siddabasave Gowda B. Gowda
    Frontiers in Aging Neuroscience, 18, Frontiers Media SA, 2026年02月11日
    研究論文（学術雑誌）, Background

    Alzheimer’s disease (AD), Parkinson’s disease (PD), and Huntington’s disease (HD) are progressive neurodegenerative diseases (NDs) characterized by chronic neuronal loss. The lack of effective treatments highlights the urgent need for reliable lipid biomarkers to enable diagnosis and monitor disease progression. Previous lipidomic investigations of altered lipid metabolism have focused on a single disease type, limiting cross-disease comparisons.

    Methods

    We applied the untargeted liquid chromatography-mass spectrometry (LC/MS) technique to profile brain lipidome alterations and to identify disease-specific lipid biomarkers across AD, HD, and PD. Brain tissue samples were collected from four cerebral lobes of healthy volunteers (HV, n  = 24) and patients diagnosed with AD ( n  = 24), PD ( n  = 24), and HD ( n  = 24). All groups include three males and three females, with brain tissues from four cortical regions sacrificed from each individual.

    Results

    A total of 243 lipid molecular species spanning five major classes were annotated, revealing distinct disease-specific lipidomic profiles that differentiated HV from the AD, HD, and PD groups via multivariate analysis. Sphingomyelins and oxidized phosphatidylserine [PS (16:1/24:0;O1)] were significantly increased, while lysophosphatidylcholines (LPC 18:2, LPC 17:2) were decreased in the AD group relative to HV. HD exhibited elevated PS (O-17:0/22:6) and ω-6 fatty acid esterified cholesteryl esters (CE 18:2, CE 20:4), alongside decreased essential neuronal lipids such as phosphatidylinositols (PI). The PD lipidome alterations closely resembled those of HD, indicating partially overlapping disruptions in brain lipid metabolism. Receiver operating characteristic analysis identified PS (16:1/24:0;O1), PS (O-17:0/22:6), and PI (18:1/18:1) as potential discriminatory biomarkers with strong diagnostic performance. Regional heatmap analysis revealed significant lipid perturbations were observed in the parietal and occipital lobes across all NDs.

    Conclusion

    This study provides a comprehensive overview of disease- and region-specific alterations in the brain lipidome of AD, HD, and PD. The identified lipid species-PS (16:1/24:0;O1), PS (O-17:0/22:6), and PI (18:1/18:1)-may serve as promising candidate biomarkers for NDs diagnosis and warrant further mechanistic and longitudinal validation with large data set.
  • Lipidomic profiling of 56 japonica rice cultivars and identification of novel fatty acid esters of hydroxy fatty acids
    Lipsa Rani Nath; Siddabasave Gowda B. Gowda; Divyavani Gowda; Perumalsamy Parasuraman; Wei Qin; Shu-Ping Hui
    Food Research International, 223, 117895, 117895, Elsevier BV, 2026年01月
    研究論文（学術雑誌）
  • Sex- and Regio-Specific Lipid Profiling of Shishamo and Capelin Fish by Nontargeted Liquid Chromatography/Mass Spectrometry
    Yusuke Minami; Siddabasave Gowda B. Gowda; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
    Foods, 2026年01月
    研究論文（学術雑誌）
  • 液体クロマトグラフィー-タンデム質量分析法による北海道産母乳中のplasmalogenの定量(Quantitation of plasmalogen in Hokkaido maternal breast milk by liquid chromatography-tandem mass spectrometry)
    陳 一凡; Ueda Rinako; Gowda Siddabasave Gowda B.; Gowda Divyavani; Miyashita Chihiro; Ikeda Atsuko; Hui Shu-Ping
    日本未病学会学術総会抄録集, 32回, jma32002, 00035, (一社)日本未病学会, 2025年11月
    英語
  • Effect of exercise on multi-compartment lipid metabolism in murine models fed an obesogenic diet using non-targeted LC/MS
    Rachana M. Gangadhara; Divyavani Gowda; Siddabasave Gowda B. Gowda; Gunjan Upadhyay; Nikhil Chainani; Vasundara Kain; Ganesh V. Halade; Shu-Ping Hui
    Frontiers in Nutrition, 12, Frontiers Media SA, 2025年10月23日
    研究論文（学術雑誌）, Background

    Chronic inflammation from obesogenic diets (OBD) disrupts lipid metabolism and contributes to non-communicable metabolic diseases. Exercise is a non-drug intervention that reduces inflammatory metabolic diseases by improving lipid metabolism. However, there are research gaps in understanding how the lipid metabolites are altered by exercise under an OBD.

    Methods

    Two-month-old male C57BL/6 J mice were fed a control (CON diet-Standard Lab Chaw-3.4%w/w Teklad Global #2918) or OBD for 10 months, then assigned to sedentary (Sed) or exercise (Exe) groups for 2 weeks. This study aims to examine changes in lipid metabolites in plasma, tissues, and feces of mice using untargeted liquid chromatography/mass spectrometry (LC/MS). Echocardiography was used to assess the impact of OBD on heart function.

    Results

    A total of 363 lipid molecular species were identified and characterized in the murine samples by retention time behavior and MS/MS spectral annotation. Multivariate analysis showed a distinct group separation between CON and OBD groups in both Sed and Exe groups. Phospholipids acylated with docosahexaenoic acid (DHA) are the key metabolites responsible for group separation in tissues and plasma, whereas in feces, glycerolipids, mainly monoacylglycerols. Lysophosphatidylethanolamine (LPE 22:5) was significantly upregulated in the liver, plasma, and left ventricle of the OBD mice in both Sed and Exe groups, contradictorily DHA containing phosphatidylglycerol [PG (22:6/22:6)] was significantly downregulated. Exercise modestly modulated the lipid profile under OBD, lowering plasma ceramides and partially reversing lipid alterations in feces. Interestingly, exercise combined with a control diet led to an increase in gut-microbiota-derived short-chain fatty acid esters of hydroxy fatty acids.

    Conclusion

    Chronic OBD induces distinct lipid alterations across multiple biological compartments. Short-term exercise provides modest improvements, with stronger benefits when combined with a balanced diet.
  • 非標的液体クロマトグラフィー/質量分析法を用いた食用キビの脂質プロファイリング(Dietary millets lipid profiling using nontargeted liquid chromatography/Mass spectrometry)
    Suraksha Kumari; Gowda Siddabasave Gowda B.; Gowda Divyavani; Nayaka S. Chandra; Hui Shu-Ping
    JSBMS Letters, 50, Suppl., 53, 53, (一社)日本医用マススペクトル学会, 2025年08月
    英語
  • LC-MS/MSによる母乳中のplasmalogenの定量(Quantitation of plasmalogen in maternal breast milk by LC-MS/MS)
    Chen Yifan; Ueda Rinako; Gowda Siddabasave Gowda B.; Gowda Divyavani; Miyashita Chihiro; Ikeda Atsuko; Hui Shu-Ping
    JSBMS Letters, 50, Suppl., 90, 90, (一社)日本医用マススペクトル学会, 2025年08月
    英語
  • Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s Plasma
    2025年07月
  • Lipidomic Profiling of Edible Japanese Sea Urchins by LC–MS
    Sahana Amai; Kisara Yuki; Siddabasave Gowda B. Gowda; Divyavani Gowda; Shu-Ping Hui
    Foods, 14, 13, 2268, 2268, MDPI AG, 2025年06月26日
    研究論文（学術雑誌）, Sea urchins (Echinoidea) are marine echinoderms commonly consumed as seafood in East Asia. To date, various metabolic components of sea urchins have been analyzed, and their health benefits for humans have also been attracting attention. Lipids are the major biomolecules present in sea urchins. However, the comprehensive lipid profiling of sea urchins is limited. In this study, we aimed to perform the comprehensive lipid profiling of six types of sea urchins using liquid chromatography–mass spectrometry (LC/MS). The application of untargeted lipidomics led to the identification of 281 lipid molecular species in six varieties of fresh sea urchin gonads. Each lipid metabolite was identified based on its retention time and MS/MS fragmentation pattern. The results of the analysis showed the highest abundance of lipid percentage in Kitamurasakiuni (14.3%), followed by Hokuyobafununi (12.4%). In all the analyzed sea urchins, glycerolipids such as triacylglycerols were found to be the most abundant lipid components. Multivariate analysis revealed that Murasakiuni showed a different lipid profile from the other types. Interestingly, the polyunsaturated fatty acid to saturated fatty acid ratios and health-related nutritional indices factors were found to be higher in Hokuyobafununi compared to other varieties. The ω-3 fatty acids, such as docosapentaenoic acid (FA 22:6) and eicosapentaenoic acid (FA 20:5), were also abundant in Hokuyobafununi. Lipids such as ether and N-acyl-type lysophosphatidylethanolamines were detected for the first time in sea urchins. This study highlights the nutritional significance of sea urchins and their potential use in the development of functional foods.
  • Discovering Novel Short- and Medium-Chain Esters of Hydroxy Fatty Acids in Human Fecal Samples Using Untargeted Liquid Chromatography/Mass Spectrometry.
    Jayashankar Jayaprakash; Divyavani Gowda; Rachana M Gangadhara; Shalini Jain; Hariom Yadav; Siddabasave Gowda B Gowda; Shu-Ping Hui
    Rapid communications in mass spectrometry : RCM, 39, 12, e10032, 2025年04月30日, ［国際誌］
    英語, 研究論文（学術雑誌）, RATIONALE: Exploring novel metabolites produced by host gut microbiome communication is crucial for understanding their roles in various disease pathologies. We previously uncovered a novel class of lipids, short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs), in mouse fecal samples and demonstrated their promising physiological functions in mammals. However, the discovery of SFAHFAs in human samples remains unexplored. METHODS: This study aimed to analyze the SFAHFAs and their structural analogs in human fecal samples using liquid chromatography/mass spectrometry. RESULTS: We identified 26 isomeric lipid species, including SFAHFAs and novel medium-chain fatty acid esters of hydroxy fatty acids (MFAHFAs). The detected SFAHFAs and MFAHFAs were characterized by accurate mass measurements using MSn analysis. The results were validated by matching the mass spectral fragmentation and retention time with authentic standards. Two new MFAHFAs, enanthic acid and caprylic acid esters of long-chain hydroxy fatty acids (C24 and C26), were detected and characterized for the first time in human fecal samples. Among the 26 isomeric lipid species, SFAHFA 2:0/24:0 or 4:0/22:0 and SFAHFA 2:0/24:1 were most abundant among the saturated and unsaturated SFAHFAs, respectively. CONCLUSIONS: This study offers the first insights into detecting and characterizing novel gut microbial lipids in human fecal samples. Further investigations are essential to recognize the metabolism and function of these lipids in the human gut.
  • シシャモおよびカラフトシシャモの健康機能性脂質の違い
    南 雄介; Bomme Gowda Siddabasave Gowda; Divyavani Gowda; 千葉 仁志; 惠 淑萍
    日本栄養・食糧学会大会講演要旨集, 79回, 233, 233, (公社)日本栄養・食糧学会, 2025年04月
    日本語
  • Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study
    Yifan Chen; Siddabasave Gowda B. Gowda; Divyavani Gowda; Jayashankar Jayaprakash; Lipsa Rani Nath; Atusko Ikeda; Yu Ait Bamai; Rahel Mesfin Ketema; Reiko Kishi; Hitoshi Chiba; Shu-Ping Hui
    Diagnostics, 15, 6, 743, 743, MDPI AG, 2025年03月16日
    研究論文（学術雑誌）, Background: Plasmalogens (Pls) are phospholipids with a unique structure, abundant in the brain and heart. Due to their chemical instability and analytical difficulties, less information is available compared to other phospholipids. The importance of Pls in several cellular processes is known, one of which is their protective effect against oxidative damage. The physiological role of Pls in human development has not been elucidated. Despite their clinical importance, the quantitative analysis of Pls in children’s plasma has been limited. Methods: This study aims to determine the plasma levels of Pls in prepubertal children using liquid chromatography/tandem mass spectrometry (LC-MS/MS). The plasma samples used were obtained from 9- to 12-year-old girls (n = 156) and boys (n = 178), n = 334 in total, who participated in the Hokkaido study. Results: Ethanolamine plasmalogen (PlsEtn) and choline plasmalogen (PlsCho), both carrying eicosapentaenoic acid, were significantly lower in girls than in boys. In both sexes, the plasmalogen levels for the 12-year-old children were lower than those for the 9-year-old children. PlsCho (16:0/18:2) was lower in the overweight children than in the normal-weight children for both sexes. PlsEtn (18:0/20:4) was the most abundant ethanolamine-type plasmalogen in both sexes. Conclusions: This study is the first report on plasmalogen levels and molecular types in children’s plasma. This study provides the information needed to understand the role of Pls in human developmental processes and may open up new opportunities in the future to control age-related changes in Pls.
  • Plasma Lipidomics of Preadolescent Children: A Hokkaido Study
    Jayashankar Jayaprakash; Siddabasave Gowda B. Gowda; Divyavani Gowda; Atsuko Ikeda; Yu Ait Bamai; Rahel Mesfin Ketema; Reiko Kishi; Yifan Chen; Hitoshi Chiba; Shu-Ping Hui
    Journal of Lipids, 2025, 3106145, 3106145, 2025年01月, ［国際誌］
    英語, 研究論文（学術雑誌）, Lipids are the most abundant biomolecules of human plasma, and their balance plays a significant role in health and disease management. Despite the importance of lipids, the studies focused on the comprehensive determination of the plasma lipidome in children are limited. In this study, we investigated the sex, age, and weight-specific changes in the plasma lipidome of nonfasting preadolescent children aged 9-12 years (n = 342) using a nontargeted liquid chromatography-mass spectrometry technique. A total of 219 lipid species were characterized in the plasma samples. Multivariate analysis revealed that boys and girls have similar lipid profiles, but relatively higher levels of capric acid-composed triacylglycerols (TGs) were observed in plasma samples of boys. Saturated fatty acids are the most abundant fatty acyls followed by mono- and polyunsaturated fatty acids in the plasma of both boys and girls. Sphingolipids such as ceramides, hexosylceramides, sphingomyelin, and a phospholipid (phosphatidylinositol) were relatively higher in the plasma of a 10-year-old group than other age groups. Plasma levels of TG and phosphatidylserine were increased within age from 9 to 12 years. Furthermore, most of the TG molecular species were increased in the plasma of overweight children compared to the normal range groups. The receiver operating characteristic analysis results show that TG (10:0/10:0/18:1) could be a specific marker for childhood obesity (area under the curve (AUC) = 0.72). Overall, this study highlights the altered plasma lipidome in preadolescent children for sex, age, and percentage of overweight. Early detection of lipid markers for obesity would be a promising target for developing therapeutic strategies.
  • Integration of lipidomics with targeted, single cell, and spatial transcriptomics defines an unresolved pro-inflammatory state in colon cancer.
    Ramani Soundararajan; Michelle M Maurin; Jetsen Rodriguez-Silva; Gunjan Upadhyay; Ashley J Alden; Siddabasave Gowda B Gowda; Michael J Schell; Mingli Yang; Noah Jhad Levine; Divyavani Gowda; Punith M Sundaraswamy; Shu-Ping Hui; Lance Pflieger; Heiman Wang; Jorge Marcet; Carolina Martinez; Robert David Bennett; Allen Chudzinski; Andreas Karachristos; Timothy M Nywening; Paul M Cavallaro; Matthew Linley Anderson; Robert J Coffey; Michael V Nebozhyn; Andrey Loboda; Domenico Coppola; Warren Jackson Pledger; Ganesh V Halade; Timothy J Yeatman
    Gut, 2024年12月20日, ［国際誌］
    英語, 研究論文（学術雑誌）, BACKGROUND: Over a century ago, Virchow proposed that cancer represents a chronically inflamed, poorly healing wound. Normal wound healing is represented by a transitory phase of inflammation, followed by a pro-resolution phase, with prostaglandin (PGE2/PGD2)-induced 'lipid class switching' producing inflammation-quenching lipoxins (LXA4, LXB4). OBJECTIVE: We explored if lipid dysregulation in colorectal cancers (CRCs) is driven by a failure to resolve inflammation. DESIGN: We performed liquid chromatography and tandem mass spectrometry (LC-MS/MS) untargeted analysis of 40 human CRC and normal paired samples and targeted, quantitative analysis of 81 human CRC and normal paired samples. We integrated analysis of lipidomics, quantitative reverse transcription-PCR, large scale gene expression, and spatial transcriptomics with public scRNASEQ data to characterize pattern, expression and cellular localisation of genes that produce and modify lipid mediators. RESULTS: Targeted, quantitative LC-MS/MS demonstrated a marked imbalance of pro-inflammatory mediators, with a dearth of resolving lipid mediators. In tumours, we observed prominent over-expression of arachidonic acid derivatives, the genes encoding their synthetic enzymes and receptors, but poor expression of genes producing pro-resolving synthetic enzymes and resultant lipoxins (LXA4, LXB4) and associated receptors. These results indicate that CRC is the product of defective lipid class switching likely related to inadequate or ineffective levels of PGE2/PGD2. CONCLUSION: We show that the lipidomic profile of CRC tumours exhibits a distinct pro-inflammatory bias with a deficiency of endogenous resolving mediators secondary to defective lipid class switching. These observations pave the way for 'resolution medicine', a novel therapeutic approach for inducing or providing resolvins to mitigate the chronic inflammation driving cancer growth and progression.
  • Analysis and functions of bioactive lipids in food
    Jayashankar Jayaprakash; Lipsa Rani Nath; Siddabasave Gowda B. Gowda; Divyavani Gowda; Shu-Ping Hui
    Discover Food, 4, 1, Springer Science and Business Media LLC, 2024年10月14日
    研究論文（学術雑誌）
  • Nontargeted Lipidomics of Sorghum Grain Reveals Novel Fatty Acid Esters of Hydroxy Fatty Acids and Cultivar Differences in Lipid Profiles
    Lipsa Rani Nath; Siddabasave Gowda B. Gowda; Thomas H. Roberts; Divyavani Gowda; Ali Khoddami; Shu-Ping Hui
    Journal of Agricultural and Food Chemistry, 72, 37, 20690, 20703, American Chemical Society (ACS), 2024年09月04日
    研究論文（学術雑誌）
  • Lipidomics of herbal tea revealed their potential lipid nutrients including novel fatty acid esters of hydroxy fatty acids
    Rachana M. Gangadhara; Siddabasave Gowda B. Gowda; Kota Yanagimoto; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
    Journal of Food Measurement and Characterization, Springer Science and Business Media LLC, 2024年08月30日
    研究論文（学術雑誌）
  • Determination of plasma lysophosphatidylethanolamines (lyso-PE) by LC-MS/MS revealed a possible relation between obesity and lyso-PE in Japanese preadolescent children: the Hokkaido study.
    Nao Inoue; Siddabasave Gowda B Gowda; Divyavani Gowda; Toshihiro Sakurai; Atsuko Ikeda-Araki; Yu Ait Bamai; Rahel Mesfin Ketema; Reiko Kishi; Hitoshi Chiba; Shu-Ping Hui
    Annals of clinical biochemistry, 45632241280352, 45632241280352, 2024年08月21日, ［国際誌］
    英語, 研究論文（学術雑誌）, BACKGROUND: Lysophosphatidylethanolamines (lyso-PEs) are the partial hydrolysis products of phosphatidylethanolamine. Although lyso-PEs are important biomarkers in various diseases, their determination is limited by the lack of simple and efficient quantification methods. This study aims to develop an improved quantitative method for the determination of lyso-PEs and its application to an epidemiological study. METHODS: Single reaction monitoring channels by collision-induced dissociation for seven lyso-PEs were established using liquid chromatography-tandem mass spectrometry. Plasma lyso-PEs were extracted with a single-phase method using an isotopically labeled internal standard for quantification. The proposed method was adopted to define lyso-PEs in plasma samples of children aged 9-12 years living in Sapporo, Japan. RESULTS: The limit of detection and limit of quantification for each lyso-PE ranged between 0.001-0.015 and 0.002-0.031 pmol/μL, respectively. Recoveries were found to be > 91% for all the species. The analysis results of children's plasma showed that the total lyso-PE concentrations in boys (n = 181) and girls (n = 161) were 11.53 and 11.00 pmol/μL (median), respectively. Participants were further classified by the percentage of overweight and subgrouped as underweight (n = 12), normal range (n = 292), or overweight (n = 38). Interestingly, the reduction of lyso-PE 16:0 and increased lyso-PE 22:6 were observed in overweight children compared with normal range (Fold change: 0.909 and 1.174, respectively). CONCLUSIONS: This study successfully established a simple quantitative method to determine lyso-PE concentrations. Furthermore, our method revealed the possible relation between plasma lyso-PEs and overweight status.
  • Quantitative determination of plasma cholesteryl ester levels in Japanese preadolescents from the Hokkaido study using liquid chromatography/tandem mass spectrometry.
    Divyavani Gowda; Siddabasave Gowda B Gowda; Atsuko Ikeda; Rahel Mesfin Ketema; Yu Ait Bamai; Reiko Kishi; Hitoshi Chiba; Shu-Ping Hui
    Steroids, 109498, 109498, 2024年08月13日, ［国際誌］
    英語, 研究論文（学術雑誌）, Cholesteryl esters (CE) are sterols comprising various fatty acyl chains attached to a cholesterol hydroxyl moiety. CEs are often considered plasma biomarkers of liver function; however, their absolute concentrations in the plasma of Japanese preadolescents have not been well explored. This study aimed to determine the plasma CE levels in Japanese preadolescents of different sexes, ages, and body weights living in Hokkaido, Japan using targeted liquid chromatography/tandem mass spectrometry. The analysis was performed on the non-fasting plasma of preadolescents aged 9-12 years (n = 339 healthy volunteers; 178 boys and 161 girls) from Sapporo, Hokkaido, Japan. The analysis results showed that the total CE levels in boys and girls were 871 ± 153 and 862 ± 96 pmol/μL, respectively. CE 18:2 (41 ± 2.9 %) was found to be the most abundant species followed by CE 18:1 (16 ± 1.5 %) and CE 16:0 (13 ± 1.1 %). The ω-3 fatty acid-containing CEs such as CE 18:3 and CE 20:5 were significantly lower in girls than in boys. Despite the different ages, CEs were tightly regulated in the plasma of children's, and the total CEs ranged between 844 and 906 pmol/μL in boys and 824 and 875 pmol/μL in girls. The participants were further classified into three groups based on their body mass index underweight (n = 237), normal weight (n = 94), and overweight (n = 8). Most of the quantified CEs were accumulated in the overweight group. Interestingly, CE 18:3 was significantly upregulated in the overweight group compared to that in the normal range, and the area under the receiver operating characteristic curve was 0.73, suggesting that it could be a possible marker for obesity. This study marks the initial investigation of absolute CE levels in the plasma of children and can help elucidate the relationship between CEs and childhood obesity.
  • Lipidomic study of kidney in a mouse model with urine flow obstruction.
    Divyavani Gowda; Md Abdul Masum; Siddabasave Gowda B Gowda; Chandra Shekhar; Md Zahir Uddin Rubel; Shunnosuke Kira; Osamu Ichii; Yasuhiro Kon; Hitoshi Chiba; Shu-Ping Hui
    Scientific reports, 14, 1, 18042, 18042, 2024年08月05日, ［国際誌］
    英語, 研究論文（学術雑誌）, Obstructed urine flow is known to cause structural and functional kidney damage leading to renal fibrosis. However, limited information is available on the change in kidney lipids during urinary tract obstruction. In this study, we investigated the change in lipidome in a mouse model with unilateral ureteral obstruction (UUO). The establishment of the UUO model was confirmed by histopathological examination using transmission electron microscopy. Untargeted liquid chromatography/mass spectrometry was carried out over a time course of 4 and 7 days. Compared to the sham control, the UUO kidney at 7 days showed dilatation of the renal tubule with loss of brush borders and thickening of the capillary endothelium. In the kidney lipidomes obtained from the UUO 7 days group compared to the control, a significant decrease of ceramide, sphingomyelin, phosphatidylcholine, lysophospholipids, and phosphatidylethanolamine was observed, whereas cholesteryl esters, free fatty acids, phosphatidylglycerol, and cardiolipins were significantly increased. The present study revealed the disturbed lipid metabolism in the UUO model, which may provide a clue to potential lipid pathways and therapeutic targets for the early stage of renal fibrosis.
  • Dissecting new lipids and their composition in herbal tea using untargeted LC/MS
    Lipsa Rani Nath; Siddabasave Gowda B. Gowda; Divyavani Gowda; Fengjue Hou; Hitoshi Chiba; Shu Ping Hui
    Food Chemistry, 447, 138941, 138941, Elsevier BV, 2024年07月
    研究論文（学術雑誌）
  • Exploration of New Lipid Nutrients and Their Characterization in Herbal Teas Using Non-Targeted Liquid Chromatography-Mass Spectrometry.
    Md Abdul Malek; Siddabasave Gowda B Gowda; Rachana M Gangadhara; Divyavani Gowda; Shu-Ping Hui
    Foods (Basel, Switzerland), 13, 12, 2024年06月14日, ［国際誌］
    英語, 研究論文（学術雑誌）, Herbal teas are blends of leaves, seeds, fruits, and flowers from various plants that provide relaxation, anti-inflammatory benefits, and immune system support for conditions such as diabetes and asthma. Despite their health benefits, comprehensive lipidomic data on herbal teas are limited in the literature. We used non-targeted liquid chromatography-linear ion trap orbitrap mass spectrometry to identify and correlate the lipid species in the following six herbal tea samples: fennel, ginger, juniper, lemon peel, orange peel, and rosehip. A total of 204 lipid molecular species were identified, and multivariate analysis revealed a significant difference between lipid species in herbal teas. Saturated fatty acids (SFAs) and polyunsaturated fatty acids (PUFAs) are significantly abundant in juniper, including ω-3 and ω-6 fatty acids, followed by fennel. Cluster correlations showed that ginger contained mainly sphingolipids and lysophospholipids, whereas fennel was rich in phospholipids. No significant variations in the content of triacylglycerols were observed in any of the herbal teas analyzed. The ratio of PUFAs to SFAs in herbal teas showed that orange peel had the highest ratio, followed by lemon peel and fennel, indicating their potential health benefits. In addition, using high-resolution mass spectrometry, various lipids such as fatty acid esters of hydroxy fatty acids and N-acyl-lysophosphatidylethanolamines were identified and characterized in these herbal teas. This study provides a comprehensive lipid analysis and detailed characterization of lipids in six herbal teas, highlighting their plausible applications in the field of nutrition and various food industries for the development of functional foods.
  • Alterations in plasma short-chain fatty acids in preadolescence children: The Hokkaido study
    Yonghan Li; Siddabasave Gowda B. Gowda; Divyavani Gowda; Atsuko Ikeda; Yu Ait Bamai; Rahel Mesfin Ketema; Reiko Kishi; Hitoshi Chiba; Shu-Ping Hui
    Journal of Chromatography B, 124191, 124191, Elsevier BV, 2024年06月
    研究論文（学術雑誌）
  • Corrigendum to "Dissecting new lipids and their composition in herbal tea using untargeted LC/MS" [Food Chemistry, 447 (2024), 138941].
    Lipsa Rani Nath; Siddabasave Gowda B Gowda; Divyavani Gowda; Fengjue Hou; Hitoshi Chiba; Shu-Ping Hui
    Food chemistry, 139745, 139745, 2024年05月24日, ［国際誌］
    英語
  • A facile method for monitoring sphingomyelin synthase activity in HeLa cells using liquid chromatography/mass spectrometry.
    Punith M Sundaraswamy; Yusuke Minami; Jayashankar Jayaprakash; Siddabasave Gowda B Gowda; Hiroyuki Takatsu; Divyavani Gowda; Hye-Won Shin; Shu-Ping Hui
    The Analyst, 2024年05月07日, ［国際誌］
    英語, 研究論文（学術雑誌）, Sphingomyelin synthase (SMS) is a sphingolipid-metabolizing enzyme involved in the de novo synthesis of sphingomyelin (SM) from ceramide (Cer). Recent studies have indicated that SMS is a key therapeutic target for metabolic diseases such as fatty liver, type 2 diabetes, atherosclerosis, and colorectal cancer. However, very few SMS inhibitors have been identified because of the limited sensitivity and selectivity of the current fluorescence-based screening assay. In this study, we developed a simple cell-based assay coupled with liquid chromatography/tandem mass spectrometry (LC-MS/MS) to screen for SMS inhibitors. HeLa cells stably expressing SMS1 or SMS2 were used for the screening. A non-fluorescent unnatural C6-Cer was used as a substrate for SMS to produce C6-SM. C6-Cer and C6-SM levels in the cells were monitored and quantified using LC-MS/MS. The activity of ginkgolic acid C15:1 (GA), a known SMS inhibitor, was measured. GA had half-maximal inhibitory concentrations of 5.5 μM and 3.6 μM for SMS1 and SMS2, respectively. To validate these findings, hSMS1 and hSMS2 proteins were optimized for molecular docking studies. In silico analyses were conducted to assess the interaction of GA with SMS1 and SMS2, and its binding affinity. This study offers an analytical approach for screening novel SMS inhibitors and provides in silico support for the experimental findings.
  • Regio-specific lipid fingerprinting of edible sea cucumbers using LC/MS.
    Yusuke Minami; Siddabasave Gowda B Gowda; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
    Food research international (Ottawa, Ont.), 184, 114253, 114253, 2024年05月, ［国際誌］
    英語, 研究論文（学術雑誌）, Sea cucumbers are a rich source of bioactive compounds and are gaining popularity as nutrient-rich seafood. They are consumed as a whole organism in Pacific regions. However, limited data are available on the comparison of their lipid composition and nutritional value. In this study, untargeted liquid chromatography/mass spectrometry was applied to comprehensively profile lipids in the skin, meat, and intestinal contents of three color-distinct edible sea cucumbers. Multivariate principal component analysis revealed that the lipid composition of the intestinal contents of red, black, and blue sea cucumbers differs from that of skin, and meats. Polyunsaturated fatty acids (PUFAs) are abundant in the intestinal contents, followed by meats of sea cucumber. Lipid nutritional quality assessments based on fatty acid composition revealed a high P:S ratio, low index of atherogenicity, and high health promotion indices for the intestinal contents of red sea cucumber, suggesting its potential health benefits. In addition, hierarchical cluster analysis revealed that the intestinal contents of sea cucumbers were relatively high in PUFA-enriched phospholipids and lysophospholipids. Ceramides are abundant in black skin, blue meat, and red intestinal content samples. Overall, this study provides the first insights into a comprehensive regio-specific profile of the lipid content of sea cucumbers and their potential use as a source of lipid nutrients in food and nutraceuticals.
  • Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS.
    Jayashankar Jayaprakash; Siddabasave Gowda B Gowda; Pradeep K Shukla; Divyavani Gowda; Lipsa Rani Nath; Hitoshi Chiba; Radhakrishna Rao; Shu-Ping Hui
    ACS omega, 9, 14, 16044, 16054, 2024年04月09日, ［国際誌］
    英語, 研究論文（学術雑誌）, Consumption of alcohol has widespread effects on the human body. The organs that are most significantly impacted are the liver and digestive system. When alcohol is consumed, it is absorbed in the intestines and processed by the liver. However, excessive alcohol use may affect gut epithelial integrity, microbiome composition, and lipid metabolism. Despite past studies investigating the effect of ethanol on hepatic lipid metabolism, the focus on colonic lipid metabolism has not been well explored. In this study, we investigated the sex-specific effect of ethanol on the colonic content lipidome in a mouse model using nontargeted liquid chromatography-mass spectrometry. Comprehensive lipidome analysis of colonic flush samples was performed using ethanol-fed (EF) and pair-fed (PF) mice of each sex. Partial least-squares discriminant analysis revealed that ethanol altered colonic lipid composition largely in male mice compared with female mice. A significant increase in free fatty acids, ceramides, and hexosylceramides and decreased phosphatidylglycerols (PG) was observed in the EF group compared to the PF group in male mice. Phosphatidylethanolamine (PE) levels were increased significantly in the EF group of both sexes compared to the PF group. The volcanic plot shows that PG (O-15:1/15:0) and PE (O-18:2/15:0) are common markers that are increased in both sexes of the EF group. In addition, decreased fatty acid esters of hydroxy fatty acids (FAHFA) were observed specifically in the EF group of female mice. Overall, a significant variation in the mice colonic content lipidome between the EF and PF groups was observed. Target pathways, such as sphingolipid metabolism in males, FAHFA in females, and PE metabolism in both sexes, were suggested. This study provides new insight into the sex-dependent lipid change associated with alcohol-induced gut-microbiota dysfunction and its potential health impacts.
  • Synthesis and quantification of short-chain fatty acid esters of hydroxy fatty acids in rat intestinal contents and fecal samples by LC-MS/MS
    Siddabasave Gowda B. Gowda; Fengjue Hou; Divyavani Gowda; Hitoshi Chiba; Kentaro Kawakami; Satoru Fukiya; Atsushi Yokota; Shu-Ping Hui
    Analytica Chimica Acta, 1288, 342145, 342145, Elsevier BV, 2024年02月
    研究論文（学術雑誌）
  • Regiospecific analysis of lipidome in the brain from mammals of land and aquatic habitats-by liquid chromatography-mass spectrometry
    Siddabasave Gowda B. Gowda; Divyavani Gowda; Fengjue Hou; Chandra Shekhar; Hitoshi Chiba; Nina Patzke; Shu-Ping Hui
    Heliyon, 9, 12, e22959, e22959, Elsevier BV, 2023年12月
    研究論文（学術雑誌）
  • Crosstalk between Lipids and Non-Alcoholic Fatty Liver Disease
    Divyavani Gowda; Chandra Shekhar; Siddabasave Gowda B. Gowda; Yifan Chen; Shu-Ping Hui
    Livers, 3, 4, 687, 708, MDPI AG, 2023年11月23日
    研究論文（学術雑誌）, Non-alcoholic fatty liver disease (NAFLD), a complex liver disorder that can result in non-alcoholic steatohepatitis, cirrhosis, and liver cancer, is the accumulation of fat in the liver seen in people due to metabolic dysfunction. The pathophysiology of NAFLD is influenced by several variables, such as metabolic dysregulation, oxidative stress, inflammation, and genetic susceptibility. This illness seriously threatens global health because of its link to obesity, insulin resistance, type 2 diabetes, and other metabolic disorders. In recent years, lipid–NAFLD crosstalk has drawn a lot of interest. Through numerous methods, lipids have been connected to the onset and advancement of the illness. The connection between lipids and NAFLD is the main topic of the current review, along with the various therapeutic targets and currently available drugs. The importance of hepatic lipid metabolism in the progression of NAFLD is summarized with the latest results in the field.
  • Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS.
    Rachana M Gangadhara; Siddabasave Gowda B Gowda; Divyavani Gowda; Ken Inui; Shu-Ping Hui
    Foods (Basel, Switzerland), 12, 14, 2023年07月21日, ［国際誌］
    英語, 研究論文（学術雑誌）, Beans, a globally significant economic and nutritional food crop, are rich in polyphenolic chemicals with potential health advantages, providing high protein, fiber, minerals, and vitamins. However, studies on the global profiling of lipids in beans are limited. We applied a non-targeted lipidomic approach based on high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (HPLC/LTQ-Orbitrap-MS) to comprehensively profile and compare the lipids in six distinct bean cultivars, namely, adzuki red beans-adzuki cultivar (ARB-AC), adzuki red beans-Benidainagon cultivar (ARB-BC), adzuki red beans-Erimoshouzu cultivar (ARB-EC), soybean-Fukuyutaka cultivar 2021 (SB-FC21), soybean-Fukuyutaka cultivar 2022 (SB-FC22), and soybean-Oosuzu cultivar (SB-OC). MS/MS analysis defined 144 molecular species from four main lipid groups. Multivariate principal component analysis indicated unique lipid compositions in the cultivars except for ARB-BC and ARB-EC. Evaluation of the concentrations of polyunsaturated fatty acid to saturated fatty acid ratio among all the cultivars showed that SB-FC21 and SB-FC22 had the highest value, suggesting they are the most beneficial for health. Furthermore, lipids such as acyl sterol glycosides were detected and characterized for the first time in these bean cultivars. Hierarchical cluster correlations revealed the predominance of ceramides in ARB-EC, lysophospholipids in SB-FC21, and glycerophospholipids in SB-OC. This study comprehensively investigated lipids and their compositions in beans, indicating their potential utility in the nutritional evaluation of beans as functional foods.
  • Changes in the Size Distributions of Oxalic Acid and Related Polar Compounds Over Northern Japan During Spring
    Dhananjay Kumar Deshmukh; Kimitaka Kawamura; Minoru Kobayashi; Divyavani Gowda
    Journal of Geophysical Research: Atmospheres, 128, 11, American Geophysical Union (AGU), 2023年05月31日
    研究論文（学術雑誌）, Abstract

    Size distributions of chemical species provide evidence for their sources and formation mechanisms. Size‐segregated aerosols with 12 sizes were collected over Northern Japan (Sapporo: 43.07°N and 141.35°E) during the spring (9 April–21 May) of 2001 and analyzed for water‐soluble organic and inorganic species. The dominances of SO42– and NH4+ in submicron (Da < 1.1 μm) and Na+ and Ca2+ in supermicron (Da > 1.1 μm) aerosols suggest substantial contributions from anthropogenic sources, sea‐salt and dust particles via long‐range atmospheric transport. Oxalic acid (C2) is the dominant organic species followed by malonic (C3) and succinic (C4) acid. A supermicron mode enrichment of C2 is heavily involved with the long‐range transport of dust particles, whereas submicron mode C2 was influenced by anthropogenic sources from the East Asian continent. The size distributions of shorter‐chain diacids (C3–C6), phthalic (Ph) and glyoxylic acid are consistent with those of C2, whereas azelaic acid (C9) is enriched in submicron and supermicron modes by the influences of continental and marine air masses. The mass concentration ratios of C3/C4 in submicron (1.5–2.3) and supermicron (1.2–1.8) mode demonstrated that water‐soluble organic aerosols in Sapporo were photochemically processed during long‐range transport. The Ph/C9 ratios show that the influence of anthropogenic sources on 9–28 April samples was pronounced in supermicron (2.5–2.9) than the submicron (1.1–1.2) particles, and vice‐versa on 6–21 May samples. These contrast distributions suggest that the continental air masses from East Asia and marine air masses from the surrounding ocean likely control the chemical composition of aerosols over Northern Japan.
  • Sulfate-associated liquid water amplifies the formation of oxalic acid at a semi-arid tropical location over peninsular India during winter
    Suresh K.R. Boreddy; Kimitaka Kawamura; Divyavani Gowda; Dhananjay K. Deshmukh; K. Narasimhulu; K. Ramagopal
    Science of The Total Environment, 874, 162365, 162365, Elsevier BV, 2023年05月
    研究論文（学術雑誌）
  • Mass spectrometric approaches in discovering lipid biomarkers for COVID-19 by lipidomics: Future challenges and perspectives.
    Siddabasave Gowda B Gowda; Chandra Shekhar; Divyavani Gowda; Yifan Chen; Hitoshi Chiba; Shu-Ping Hui
    Mass spectrometry reviews, 2023年04月27日, ［国際誌］
    英語, 研究論文（学術雑誌）, Coronavirus disease 2019 (COVID-19) has emerged as a global health threat and has rapidly spread worldwide. Significant changes in the lipid profile before and after COVID-19 confirmed the significance of lipid metabolism in regulating the response to viral infection. Therefore, understanding the role of lipid metabolism may facilitate the development of new therapeutics for COVID-19. Owing to their high sensitivity and accuracy, mass spectrometry (MS)-based methods are widely used for rapidly identifying and quantifying of thousands of lipid species present in a small amount of sample. To enhance the capabilities of MS for the qualitative and quantitative analysis of lipids, different platforms have been combined to cover a wide range of lipidomes with high sensitivity, specificity, and accuracy. Currently, MS-based technologies are being established as efficient methods for discovering potential diagnostic biomarkers for COVID-19 and related diseases. As the lipidome of the host cell is drastically affected by the viral replication process, investigating lipid profile alterations in patients with COVID-19 and targeting lipid metabolism pathways are considered to be crucial steps in host-directed drug targeting to develop better therapeutic strategies. This review summarizes various MS-based strategies that have been developed for lipidomic analyzes and biomarker discoveries to combat COVID-19 by integrating various other potential approaches using different human samples. Furthermore, this review discusses the challenges in using MS technologies and future perspectives in terms of drug discovery and diagnosis of COVID-19.
  • Temporal lipid profiling in the progression from acute to chronic heart failure in mice and ischemic human hearts
    Gowda, S.G.B.; Gowda, D.; Hou, F.; Chiba, H.; Parcha, V.; Arora, P.; Halade, G.V.; Hui, S.-P.
    Atherosclerosis, 363, 30, 41, Elsevier BV, 2022年12月
    研究論文（学術雑誌）
  • Detection and characterization of lipids in eleven species of fish by non-targeted liquid chromatography/mass spectrometry
    Siddabasave Gowda B. Gowda; Yusuke Minami; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
    Food Chemistry, 393, 133402, 133402, Elsevier BV, 2022年11月, ［国際誌］
    英語, 研究論文（学術雑誌）, Fish is an important nutrition source because its lipids, which are rich in ω-3 fatty acids, are beneficial for human health. However, studies focusing on their detection, composition, and nutritional value are limited. In this study, we applied a non-targeted lipidomic approach based on ultra-high performance liquid chromatography coupled with linear-ion trap-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap-MS) to comprehensively profile, compare, and detect unknown lipids in eleven types of dietary fish. A total of 287 molecular species from five major lipid classes were characterized by MS/MS analysis. Multivariate principal component analysis revealed the distinct lipid composition in shishamo smelt and Japanese sardine compared to other fish types. The assessment of nutritional indices based on the levels of free fatty acid suggested that among the eleven fish types, shishamo smelt is highly beneficial for health. Further, lipids such as N-acyl lysophosphatidylethanolamine were detected and characterized for the first time in fish fillets. Hierarchical cluster correlations indicated the predominance of glycerophospholipids (GPs) and sphingolipids in sardine, whereas fatty acyls and triacylglycerols (TAGs) were predominant in shishamo smelt. The high levels of polyunsaturated fatty acid-enriched GPs and TAGs in dietary fish endow it with great potential as a health-promoting food for human consumption. This study offers a comprehensive analysis of lipids and their compositions in fish fillets, demonstrating their potential use in the nutritional assessment of functional foods.
  • 食事摂取した海藻の脂質に関する液体クロマトグラフィー/質量分析計を利用した探索的なデータ分析研究(Exploratory data analysis on dietary seaweeds lipids by liquid chromatography/mass spectrometry)
    Gowda Siddabasave Gowda B.; Yifan Chen; Gowda Divyavani; Tsuboi Yui; Chiba Hitoshi; Hui Shu-Ping
    日本未病学会学術総会抄録集, 29回, 73, 73, (一社)日本未病学会, 2022年10月
    英語
  • Determination of short-chain fatty acids by N,N-dimethylethylenediamine derivatization combined with liquid chromatography/mass spectrometry and their implication in influenza virus infection
    Divyavani Gowda; Yonghan Li; Siddabasave Gowda B Gowda; Marumi Ohno; Hitoshi Chiba; Shu-Ping Hui
    Analytical and Bioanalytical Chemistry, 414, 22, 6419, 6430, Springer Science and Business Media LLC, 2022年09月, ［国際誌］
    英語, 研究論文（学術雑誌）, Short-chain fatty acids (SCFAs) are the end products of the fermentation of complex carbohydrates by the gut microbiota. Although SCFAs are recognized as important markers to elucidate the link between gut health and disease, it has been difficult to analyze SCFAs with mass spectrometry technologies due to their poor ionization efficiency and high volatility. Here, we present a novel and sensitive method for the quantification of SCFAs, including C2-C6 SCFAs and their hydroxy derivatives, by liquid chromatography/tandem mass spectrometry (LC-MS/MS) upon N,N-dimethylethylenediamine (DMED) derivatization with a run time of 10 min. Moreover, the quantification method of DMED-derivatized SCFAs in intestinal contents using isotope-labeled internal standards was also established. The method validation was performed by analyzing spiked intestinal samples; the limits of detection and quantification of SCFAs with this method were found to be 0.5 and 5 fmol, respectively; the recovery was greater than 80% and good linearity (0.9932 to 0.9979) of calibration curves was obtained over the range from 0.005 to 5000 pmol/μL; the intraday and interday precisions were achieved in the range of 1-5%. Furthermore, the validated method was applied to analyze SCFAs in the cecum and colon contents of mice infected with the influenza virus. The results showed that the concentration of most of the SCFAs tested here decreased significantly in a time-dependent manner after the infection, suggesting a possibility that SCFAs in intestinal samples could be used as severe disease markers. Overall, we here successfully developed a simple, fast, and sensitive method for SCFA analysis by LC-MS/MS combined with DMED derivatization. The method for the quantification of SCFAs will be a useful tool for both basic research and clinical studies.
  • Analysis of Antioxidant Lipids in Five Species of Dietary Seaweeds by Liquid Chromatography/Mass Spectrometry
    Siddabasave Gowda B. Gowda; Chen Yifan; Divyavani Gowda; Yui Tsuboi; Hitoshi Chiba; Shu-Ping Hui
    Antioxidants, 11, 8, 1538, 1538, MDPI AG, 2022年08月08日, ［国際誌］
    英語, 研究論文（学術雑誌）, Seaweeds are a good source of bioactive lipids and are known for their nutritional benefits, making them a valuable food source. Despite their dietary significance and nutritional importance, there are limited reports on comprehensive lipidome analysis of lipids with antioxidant properties. Therefore, this study aimed to compare the lipid profiles of five commonly consumed Japanese dietary seaweeds using non-targeted liquid chromatography/mass spectrometry (LC/MS). A total, of 304 molecular species from four major lipid classes were detected and characterized by MS/MS analysis. Multivariate statistical analysis revealed distinct lipid molecular compositions in kombu and sea mustard compared to hijiki, mozuku, and laver seaweeds. Kombu has been shown to contain large amounts of antioxidants, such as polyunsaturated fatty acids (PUFAs), and a high health promotion index compared to other seaweeds. Hierarchical cluster correlations indicated the predominance of glycerophospholipids (GPs) and glycerolipids (GLs) in sea mustard and kombu. As a result, dietary seaweeds have great potential as antioxidants and health-promoting foods for human consumption due to their high levels of PUFA-rich GPs and GLs. Unsaturated triacylglycerols are predominant in hijiki, whereas other health-beneficial lipids, such as monogalactosyldiacylglycerol and sulfoquinovosyl diacylglycerols, are predominant in sea mustard. This study provides a detailed characterization of lipids and their comparative fingerprints in seaweeds, demonstrating the potential use of dietary seaweeds in biotechnological and industrial applications involving the development of functional food products.
  • Simple and Sensitive Method for the Quantitative Determination of Lipid Hydroperoxides by Liquid Chromatography/Mass Spectrometry
    Chongsheng Liang; Siddabasave Gowda B. Gowda; Divyavani Gowda; Toshihiro Sakurai; Iku Sazaki; Hitoshi Chiba; Shu-Ping Hui
    Antioxidants, 11, 2, 229, 229, MDPI AG, 2022年01月25日
    研究論文（学術雑誌）
  • Defining the kinetic effects of infection with influenza virus A/PR8/34 (H1N1) on sphingosine-1-phosphate signaling in mice by targeted LC/MS
    Divyavani Gowda; Marumi Ohno; Siddabasave Gowda B. Gowda; Hitoshi Chiba; Masashi Shingai; Hiroshi Kida; Shu-Ping Hui
    Scientific Reports, 11, 1, 20161, 20161, Springer Science and Business Media LLC, 2021年12月, ［国際誌］
    英語, 研究論文（学術雑誌）, AbstractInfluenza remains a world-wide health concern, causing 290,000–600,000 deaths and up to 5 million cases of severe illnesses annually. Noticing the host factors that control biological responses, such as inflammatory cytokine secretion, to influenza virus infection is important for the development of novel drugs. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite and has essential biological functions in inflammation. However, the kinetic effects of influenza virus infection on physiological S1P levels and their signaling in multiple tissues remain unknown. In this study, we utilized a mouse model intranasally infected with 50 or 500 plaque forming units (PFU) of A/Puerto Rico/8/34 (H1N1; PR8) virus to investigate how S1P levels and expression of its regulating factors are affected by influenza virus infection by the liquid-chromatography/mass spectrometry and real-time PCR, respectively. The S1P level was significantly high in the plasma of mice infected with 500 PFU of the virus than that in control mice at 6 day-post-infection (dpi). Elevated gene expression of sphingosine kinase-1 (Sphk1), an S1P synthase, was observed in the liver, lung, white adipose tissue, heart, and aorta of infected mice. This could be responsible for the increased plasma S1P levels as well as the decrease in the hepatic S1P lyase (Sgpl1) gene in the infected mice. These results indicate modulation of S1P-signaling by influenza virus infection. Since S1P regulates inflammation and leukocyte migration, it must be worth trying to target this signaling to control influenza-associated symptoms.
  • Sphingosine-1-phosphate interactions in the spleen and heart reflect extent of cardiac repair in mice and failing human hearts
    SiddabasaveGowda B. Gowda; Divyavani Gowda; Vasundhara Kain; Hitoshi Chiba; Shu-Ping Hui; Charles E. Chalfant; Vibhu Parcha; Pankaj Arora; Ganesh V. Halade
    American Journal of Physiology-Heart and Circulatory Physiology, 321, 3, H599, H611, American Physiological Society, 2021年09月01日
    研究論文（学術雑誌）, Previous studies indicate that sphingosine-1-phosphate (S1P) has some role in cardiovascular disease. This study adds quantitative and integrative systems-based approaches that are necessary for discovery and bedside translation. Here, we quantitated sphinganine, sphingosine, sphingosine-1-phosphate (S1P) in mice and human cardiac pathobiology. Interorgan S1P quantity and respective systems-based receptor activation suggest cardiac repair after myocardial infarction. Thus, S1P serves as a therapeutic target for cardiac protection in clinical translation.
  • Detection and Structural Characterization of SFAHFA Homologous Series in Mouse Colon Contents by LTQ-Orbitrap-MS and Their Implication in Influenza Virus Infection
    Siddabasave Gowda B. Gowda; Divyavani Gowda; Marumi Ohno; Chongsheng Liang; Hitoshi Chiba; Shu-Ping Hui
    Journal of the American Society for Mass Spectrometry, 32, 8, 2196, 2205, American Chemical Society (ACS), 2021年08月04日, ［国際誌］
    英語, 研究論文（学術雑誌）, Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with promising physiological functions in mammals. We previously introduced a new type of lipids to this family called short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs), branching specific to the C2 carbon of a long-chain fatty acid (≥C20). In this study, we discovered a homologous series of SFAHFAs comprising C16-C26 hydroxy fatty acids esterified with short-chain fatty acids (C2-C5) in mouse colon contents. The detected SFAHFAs were characterized by high-resolution mass spectrometry with MSn analysis. The double-bond position of monounsaturated SFAHFAs was determined by the epoxidation reaction of samples with m-chloroperoxybenzoic acid and their MSn analysis. Further, the measurement of SFAHFA concentration in the colon contents of mice infected with influenza A/Puerto Rico/8/34 (H1N1; PR8) virus revealed a significant increase in their levels compared to native control. A strong correlation was observed between hydroxy fatty acid and SFAHFAs. Detection, characterization, and profiling of these new SFAHFA levels in relation with pandemic H1N1; PR8 influenza virus will contribute to the in-depth study of their function and metabolism.
  • Docosahexaenoic Acid Esters of Hydroxy Fatty Acid Is a Novel Activator of NRF2
    Siddabasave Gowda B. Gowda; Takayuki Tsukui; Hirotoshi Fuda; Yusuke Minami; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
    International Journal of Molecular Sciences, 22, 14, 7598, 7598, MDPI AG, 2021年07月15日, ［国際誌］
    英語, 研究論文（学術雑誌）, Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with interesting physiological functions in mammals. Despite their structural diversity and links with nuclear factor erythroid 2-related factor 2 (NRF2) biosynthesis, FAHFAs are less explored as NRF2 activators. Herein, we examined for the first time the synthetic docosahexaenoic acid esters of 12-hydroxy stearic acid (12-DHAHSA) or oleic acid (12-DHAHOA) against NRF2 activation in cultured human hepatoma-derived cells (C3A). The effect of DHA-derived FAHFAs on lipid metabolism was explored by the nontargeted lipidomic analysis using liquid chromatography-mass spectrometry. Furthermore, their action on lipid droplet (LD) oxidation was investigated by the fluorescence imaging technique. The DHA-derived FAHFAs showed less cytotoxicity compared to their native fatty acids and activated the NRF2 in a dose-dependent pattern. Treatment of 12-DHAHOA with C3A cells upregulated the cellular triacylglycerol levels by 17-fold compared to the untreated group. Fluorescence imaging analysis also revealed the suppression of the degree of LDs oxidation upon treatment with 12-DHAHSA. Overall, these results suggest that DHA-derived FAHFAs as novel and potent activators of NRF2 with plausible antioxidant function.
  • Lipidomic analysis of non-esterified furan fatty acids and fatty acid compositions in dietary shellfish and salmon by UHPLC/LTQ-Orbitrap-MS
    Siddabasave Gowda B. Gowda; Yusuke Minami; Divyavani Gowda; Daisuke Furuko; Hitoshi Chiba; Shu-Ping Hui
    Food Research International, 144, 110325, 110325, Elsevier BV, 2021年06月, ［国際誌］
    英語, 研究論文（学術雑誌）, Lipids such as furan fatty acids (F-acids) are the valuable minor bioactive components of food such as fatty fish and plants. They are reported to have positive health benefits, including antioxidant and anti-inflammatory activities. Despite their importance, limited studies are focusing on F-acid determination in dietary seafood. This study aimed to identify and profile non-esterified F-acids and free fatty acids in total lipid extract of seafood such as shellfish and salmon. The lipidomic analysis using liquid chromatography-linear trap quadrupole-orbitrap mass spectrometry led to identifying seven types of free F-acids in shellfish (n = 5) and salmon (n = 4). The identified F-acids were confirmed by their high-resolution masses and acquired mass spectra. The relative concentrations of F-acids in shellfish range from 0.01 to 10.93 mg/100 g of the fillet, and in salmon, 0.01 to 14.21 mg/100 g of the fillet. The results revealed the highest abundance of F-acids in Sakhalin surf clam, Japanese scallop, and a fatty salmon trout. Besides, relative levels of saturated, monounsaturated, and polyunsaturated fatty acids (PUFAs) in these seafoods were compared with each other, suggesting basket clams and salmon trout to have significantly higher levels of PUFAs. The dietary seafoods enriched with F-acids and PUFAs may have possible health benefits. Hence, the applied technique could be a promising tool for rapid detection and analysis of non-esterified fatty acids in food.
  • Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents.
    Siddabasave Gowda B Gowda; Divyavani Gowda; Chongsheng Liang; Yonghan Li; Kentaro Kawakami; Satoru Fukiya; Atsushi Yokota; Hitoshi Chiba; Shu-Ping Hui
    Metabolites, 10, 10, 2020年10月08日, ［国際誌］
    英語, 研究論文（学術雑誌）, Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.
  • Identification of short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs) in a murine model by nontargeted analysis using ultra-high-performance liquid chromatography/linear ion trap quadrupole-Orbitrap mass spectrometry.
    Siddabasave Gowda B Gowda; Chongsheng Liang; Divyavani Gowda; Fengjue Hou; Kentaro Kawakami; Satoru Fukiya; Atsushi Yokota; Hitoshi Chiba; Shu-Ping Hui
    Rapid communications in mass spectrometry : RCM, 34, 17, e8831, 2020年09月15日, ［国際誌］
    英語, 研究論文（学術雑誌）, RATIONALE: Fatty acid esters of hydroxy fatty acids (FAHFAs) are recently discovered endogenous lipids with outstanding health benefits. FAHFAs are known to exhibit antioxidant, antidiabetic and anti-inflammatory properties. The number of known long-chain FAHFAs in mammalian tissues and dietary resources increased recently because of the latest developments in high-resolution tandem mass spectrometry techniques. However, there are no reports on the identification of short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs). METHODS: Intestinal contents, tissues, and plasma of rats fed with high-fat diet (HFD) and normal diet (ND) were analyzed for fatty acids, hydroxy fatty acids, and FAHFAs using ultra-high-performance liquid chromatography (UHPLC) and linear trap quadrupole-Orbitrap mass spectrometry (LTQ Orbitrap MS) with negative heated electrospray ionization. RESULTS: Untargeted analysis of total lipid extracts from murine samples (male 13-week-old WKAH/HKmSlc rats) led to the identification of several new SFAHFAs of acetic acid or propanoic acid esterified long-chain (>C20)-hydroxy fatty acids. Furthermore, MS3 analysis revealed the position of the hydroxyl group in the long-chain fatty acid as C-2. The relative amounts of SFAHFAs were quantified in intestinal contents and their tissues (Cecum, small intestine, and large intestine), liver, and plasma of rats fed with HFD and ND. The large intestine showed the highest abundance of SFAHFAs with a concentration range from 0.84 to 57 pmol/mg followed by the cecum with a range of 0.66 to 28.6 pmol/mg. The SFAHFAs were significantly altered between the HFD and ND groups, with a strong decreasing tendency under HFD conditions. CONCLUSIONS: Identification of these novel SFAHFAs can contribute to a better understanding of the chemical and biological properties of individual SFAHFAs and their possible sources in the gut, which in turn helps us tackle the role of these lipids in various metabolic diseases.
  • Identification of secondary fatty alcohols in atmospheric aerosols in temperate forests
    Yuzo Miyazaki; Divyavani Gowda; Eri Tachibana; Yoshiyuki Takahashi; Tsutom Hiura
    BIOGEOSCIENCES, 16, 10, 2181, 2188, 2019年05月
    英語, 研究論文（学術雑誌）
  • Seasonal variations of low molecular weight hydroxy-dicarboxylic acids and oxaloacetic acid in remote marine aerosols from Chichijima Island in the western North Pacific (December 2010-November 2011)
    Divyavani Gowda; Kimitaka Kawamura
    ATMOSPHERIC RESEARCH, 204, 128, 135, 2018年05月
    英語, 研究論文（学術雑誌）
  • Identification of hydroxy- and keto-dicarboxylic acids in remote marine aerosols using gas chromatography/quadruple and time-of-flight mass spectrometry
    Divyavani Gowda; Kimitaka Kawamura; Eri Tachibana
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 30, 7, 992, 1000, 2016年04月, ［国際誌］
    英語, 研究論文（学術雑誌）
■ その他活動・業績
■ 主な担当授業
  • 代謝分析化学特論, 2024年, 修士課程, 保健科学院
  • 代謝分析化学演習, 2024年, 修士課程, 保健科学院
  • 英語演習, 2024年, 学士課程, 全学教育
■ 共同研究・競争的資金等の研究課題
  • 乳児用調製粉乳は母乳中のコレステロールエステル濃度を模倣しますか?
    第11回北海道大学部局横断シンポジウム, 研究助成・奨励賞
    2025年11月 - 2026年03月
    11th Hokkaido University Interdepartmental Symposium Research, Research grant and encouragement award, 研究代表者
  • ルイボスティー抽出物が肥満に伴う脂質代謝に及ぼす影響の解明
    2024年10月 - 2025年03月
    保健科学研究院若手教員奨励助成収支決算報告書
  • Unrevealing the anti-obesity effects of wakame associated to sphingomyelin synthase inhibition
    科学研究費助成事業
    2022年04月 - 2024年03月
    Divyavani Gowda
    2022年度 若手研究, 2022年度 若手研究, Hokkaido University, 研究代表者, 22K14850
  • 札幌の大気エアロゾル中の酸化多環芳香族炭化水素の定量とそれらのin vitro影響
    2022年07月 - 2023年03月
    The Collaborative Research of Next-Generation Researchers, 87051M
  • Analysis of hazardous components(PAHs) and their unknown derivatives in atmospheric PM 2.5 organic aerosols collected over Hokkaido
    科学研究費助成事業
    2020年09月11日 - 2022年03月31日
    DIVYAVANI
    エアロゾルは、気体中に浮遊する微小な粒子から構成されています。近年、粒子状物質（PM）は世界的な健康上の関心事となっています。大気中のPMは気候と健康の両方に影響を与え、PMは喘息や呼吸器疾患など、人間の健康に大きな悪影響を与える可能性があります。したがって、PAHの多様な影響を検討し、制御することができるように、その存在量、化学種、分布、潜在的な発生源を理解することが重要である。
    日本学術振興会, 研究活動スタート支援, 北海道大学, 20K23359