研究者データベース

小野 清美(オノ キヨミ)
低温科学研究所 生物環境部門
助教

基本情報

所属

  • 低温科学研究所 生物環境部門

職名

  • 助教

学位

  • 博士(理学)

科研費研究者番号

  • 50344502

J-Global ID

研究キーワード

  • 植物生理生態学   Plant ecophysiology   

研究分野

  • ライフサイエンス / 生態学、環境学

学歴

  • 1993年04月 - 1997年03月   東京大学   生物科学専攻
  • 1993年04月 - 1997年03月   東京大学

所属学協会

  • 日本光合成学会   日本生態学会   日本植物生理学会   日本植物学会   

研究活動情報

論文

  • Bihe Kong, Lei Chen, Yasuhiro Kasahara, Akihiro Sumida, Kiyomi Ono, Jan Wild, Arata Nagatake, Ryusuke Hatano, Toshihiko Hara
    MICROBES AND ENVIRONMENTS 32 2 103 - 111 2017年06月 [査読有り][通常論文]
     
    In order to understand the relationships between understory bamboo and soil properties, we compared microbial community structures in the soil of a Betula ermanii boreal forest with Sasa kurilensis present and removed using high-throughput DNA sequencing. The presence of understory S. kurilensis strongly affected soil properties, including total carbon, total nitrogen, nitrate, and the C:N ratio as well as relative soil moisture. Marked differences were also noted in fungal and bacterial communities between plots. The relative abundance of the fungal phylum Ascomycota was 13.9% in the Sasa-intact plot and only 0.54% in the Sasa-removed plot. Among the Ascomycota fungi identified, the most prevalent were members of the family Pezizaceae. We found that the abundance of Pezizaceae, known to act as mycorrhizal fungi, was related to the amount of total carbon in the Sasa-intact plot. The relative abundance of Proteobacteria was significantly higher, whereas those of Planctomycetes and Actinobacteria were lower in the Sasa-intact plot than in the Sasa-removed plot. Furthermore, the results obtained suggest that some species of the phylum Planctomycetes are more likely to occur in the presence of S. kurilensis. Collectively, these results indicate that the presence of S. kurilensis affects microbial communities and soil properties in a B. ermanii boreal forest.
  • Wataru Ishizuka, Kiyomi Ono, Toshihiko Hara, Susumu Goto
    FRONTIERS IN PLANT SCIENCE 6 890. Doi: 10.3389/fpls.2015.0  2015年10月 [査読有り][通常論文]
     
    Boreal coniferous species with wide geographic distributions show substantial variation in autumn cold acclimation among populations. To determine how this variation is inherited across generations, we conducted a progeny test and examined the development of cold hardening in open-pollinated second-generation (F-2) progeny of Abies sachalinensis. The F1 parents had different genetic backgrounds resulting from reciprocal interpopulational crosses between low elevation (L) and high elevation (H) populations: L x L, L x H, H x L, and H x H. Paternity analysis of the F-2 progeny using molecular genetic markers showed that 91.3% of the fathers were located in surrounding stands of the F-1 planting site (i.e., not in the F1 test population). The remaining fathers were assigned to F-1 parents of the L x L cross-type. This indicates that the high-elevation genome in the F-1 parents was not inherited by the F-2 population via pollen flow. The timing of autumn cold acclimation in the F-2 progeny depended on the cross type of the F1 mother. The progeny of H x H mothers showed less damage in freezing tests than the progeny of other cross-types. Statistical modeling supported a linear effect of genome origin. In the best model, variation in freezing damage was explained by the proportion of maternally inherited high-elevation genome. These results suggest that autumn cold acclimation was partly explained by the additive effect of the responsible maternal genome. Thus, the offspring that inherited a greater proportion of the high-elevation genome developed cold hardiness earlier. Genome-based variation in the regulation of autumn cold acclimation matched the local climatic conditions, which may be a key factor in elevation dependent adaptation.
  • 北海道の亜高山帯ダケカンバ林の下層チシマザサ(Sasa kurilensis (Rupr.) Makino & Shibata)の総生産および蒸散の季節変化に及ぼす環境要因
    山口貴広, 隅田明洋, 中井太郎, 山田雅仁, 児玉裕二, 小野清美, 原登志彦
    低温科学 73 41 - 56 2015年03月 [査読有り][通常論文]
  • 北方針葉樹林におけるトウヒ属Piceaとモミ属Abiesの稚樹の動態に及ぼす林床環境の影響
    西村尚之, 赤路康朗, 鈴木智之, 長谷川成明, 小野清美, 隅田明洋, 原登志彦, 飯田滋生, 関剛, 倉本惠生, 杉田久志, 中川弥智子, 松下通也, 廣部宗, 星野大介, 稲永路子, 山本進一
    低温科学 73 7 - 19 2015年03月 [査読有り][通常論文]
  • ミズナラ実生の初期成長における生育条件の影響と葉の生理的応答
    津田 元, 小野 清美, 隅田 明洋, 原 登志彦
    低温科学 73 57 - 64 2015年03月 [査読有り][通常論文]
  • W. Ishizuka, K. Ono, T. Hara, S. Goto
    PLANT BIOLOGY 17 1 177 - 185 2015年01月 [査読有り][通常論文]
     
    To avoid winter frost damage, evergreen coniferous species develop cold hardiness with suitable phenology for the local climate regime. Along the elevational gradient, a genetic cline in autumn phenology is often recognised among coniferous populations, but further quantification of evolutionary adaptation related to the local environment and its responsible signals generating the phenological variation are poorly understood. We evaluated the timing of cold hardening among populations of Abies sachalinensis, based on time series freezing tests using trees derived from four seed source populations x three planting sites. Furthermore, we constructed a model to estimate the development of hardening from field temperatures and the intraspecific variations occurring during this process. An elevational cline was detected such that high-elevation populations developed cold hardiness earlier than low-elevation populations, representing significant genetic control. Because development occurred earlier at high-elevation planting sites, the genetic trend across elevation overlapped with the environmental trend. Based on the trade-off between later hardening to lengthen the active growth period and earlier hardening to avoid frost damage, this genetic cline would be adaptive to the local climate. Our modelling approach estimated intraspecific variation in two model components: the threshold temperature, which was the criterion for determining whether the trees accumulated the thermal value, and the chilling requirement for trees to achieve adequate cold hardiness. A higher threshold temperature and a lower chilling requirement could be responsible for the earlier phenology of the high-elevation population. These thermal responses may be one of the important factors driving the elevation-dependent adaptation of A.sachalinensis.
  • M. Mimura, K. Ono, K. Goka, T. Hara
    BIOLOGICAL INVASIONS 15 12 2743 - 2754 2013年12月 [査読有り][通常論文]
     
    Although ecological differences between native and introduced ranges have been considered to drive rapid expansion of invasive species, recent studies suggest that rapid evolutionary responses of invasive species to local environments may also be common. Such expansion across heterogeneous environments by adaptation to local habitats requires genetic variation. In this study, we investigated the source and role of standing variation in successful invasion of heterogeneous abiotic environments in a self-incompatible species, Lotus corniculatus. We compared phenotypic and genetic variation among cultivars, natives, and introduced genotypes, and found substantial genetic variation within both native and introduced populations. Introduced populations possessed genotypes derived from both cultivars and native populations, and had lower population differentiation, indicating multiple sources of introduction and population admixture among the sources in the introduced range. Both cultivars and introduced populations had similarly outperforming phenotypes on average, with increased biomass and earlier flowering compared with native populations, but those phenotypes were within the range of the variation in phenotypes of the native populations. In addition, clinal variation within introduced populations was detected along a climatic gradient. Multiple introductions from different sources, including cultivars, may have contributed to pre-adaptive standing variation in the current introduced populations. We conclude that both introduction of cultivar genotypes and natural selection in local environments contributed to current patterns of genetic and phenotypic variation observed in the introduced populations.
  • 長田 典之, 及川 真平, 宮田 理恵, 神山 千穂, 永野 聡一郎, 塩寺 さとみ, 田畑 あずさ, 小野 清美
    日本生態学会誌 63 1 19 - 36 2013年03月 [査読有り][通常論文]
  • 小野 清美, 永野 聡一郎
    日本生態学会誌 63 1 49 - 57 2013年03月 [査読有り][通常論文]
  • Edgard A. Bontempo e Silva, Shigeaki F. Hasegawa, Kiyomi Ono, Akihiro Sumida, Shigeru Uemura, Toshihiko Hara
    ECOLOGICAL RESEARCH 27 5 933 - 943 2012年09月 [査読有り][通常論文]
     
    Abies sachalinensis and Picea glehnii are co-dominant tree species and major components of the forests of Hokkaido, Japan. Recent work suggests that a reversal in potential competitive superiority at different developmental stages could be important to explain their coexistence. Such shifts in competitive advantage can be mechanistically understood by studying the corresponding physiological differences between distinct life stages. Accordingly, our objective was to investigate in the field the photosynthesis of shade-growing juveniles of these species from two different size-classes, seedlings and saplings. Our results show that seedlings of both species had higher concentrations of photoprotective xanthophylls than saplings, especially in spring, and suggest that seedlings have a lower threshold of stress tolerance than saplings. Photosynthetic capacity per needle area and lateral shoot extension rate decreased from the seedling to the sapling stage in A. sachalinensis, while in P. glehnii, both increased from the seedling to the sapling stage. Abies sachalinensis had higher photosynthetic rates at the seedling stage but lower rates at the sapling stage than P. glehnii. Nevertheless, A. sachalinensis had a higher lateral shoot extension rate than P. glehnii at both stages. Our physiological results support previous ecological observations that A. sachalinensis is a superior competitor to P. glehnii in the understory, and show that its competitive advantage is higher at the seedling stage than at the sapling stage.
  • Azusa Tabata, Kiyomi Ono, Akihiro Sumida, Toshihiko Hara
    ECOLOGICAL RESEARCH 25 4 823 - 835 2010年07月 [査読有り][通常論文]
     
    Four 2-year watering treatments were applied to Betula ermanii seedlings to investigate their responses to soil water conditions: ID [irrigated well (I) in the previous year and water deficit (D) in the current year], DD, DI, and II. RGR of the seedlings in current-year water deficit without experiencing previous-year water deficit (ID) was smaller than that of seedlings irrigated well in both years (II). Surprisingly, RGR did not differ between 2-year-water-deficited (DD) and -well-irrigated (II) treatments. There was no difference in the area-based photosynthetic rate of the late leaves, which are formed in the current-year environment, between the four water treatments, but their leaf longevity was shortened by water deficit. Area-based photosynthetic rate of the early leaves, whose buds are formed in the previous year environment, was smaller in ID than in II, but it did not differ between DD and II. The de-epoxidation state of the xanthophyll cycle was greater in ID than in DD. Leaf mass per area (leaf dry weight/leaf area) did not differ among the four water treatments. Specific root length (root total length/root dry weight) did not differ between ID and II. It was greater in DD than in II, probably resulting in increased water uptake efficiency. Photoprotective systems such as xanthophyll contents and antioxidant enzyme activities did not differ among the four treatments. Morphological responses of B. ermanii roots seem to be important as whole-plant-level responses to water deficit for maintaining RGR, in addition to leaf-level photosynthetic and phenological responses of two types of leaves. Our results partly explain how B. ermanii seedlings survive and maintain growth even under varying soil water conditions in the boreal forest.
  • J. -J. Bae, Y. -S. Choo, K. Ono, A. Sumida, T. Hara
    PHOTOSYNTHETICA 48 1 110 - 116 2010年03月 [査読有り][通常論文]
     
    The response of Picea glehnii, a cold-tolerant species in the boreal zone, to air temperature (T) was investigated for its cold-acclimated needles (i.e. the ones subjected to gradual decrease in T) and nonacclimated needles (i.e. the ones subjected to a sudden decrease in T) were compared under low temperature. Cold-acclimated needles showed a greater increase of zeaxanthin and lutein contents than nonacclimated ones, whereas the nonacclimated needles showed a greater increase of thylakoid-bound ascorbate peroxidase (tAPX) activity than cold-acclimated ones under chilling conditions (after cold acclimation). These results suggest that: (1) low T induces the increase of zeaxanthin and lutein content, and tAPX activity; (2) accumulated zeaxanthin and lutein protect needles from photooxidative stress by dissipating excess energy before the reactive oxygen species (ROS) are formed in response to a gradual decrease in T (with cold acclimation and subsequent chilling condition), and by tAPX scavenging ROS formed in the case of a sudden decrease in T (without cold acclimation and chilling condition).
  • Naoyuki Nishimura, Kyoko Kato, Akihiro Sumida, Kiyomi Ono, Hiroyuki Tanouchi, Shigeo Iida, Daisuke Hoshino, Shin-Ichi Yamamoto, Toshihiko Hara
    PLANT ECOLOGY 206 1 29 - 40 2010年01月 [査読有り][通常論文]
     
    We examined the effects of different life history strategies and tree competition on species coexistence in a northern coniferous forest. We investigated the growth and demography of trees with stems a parts per thousand yen1 cm dbh in a 2-ha study plot in the Taisetsu Mountains of northern Japan. Three species, Abies sachalinensis, Picea jezoensis, and Picea glehnii, were found to be dominant in the forest. A. sachalinensis was the most dominant species in the understory, while the two Picea spp. were more abundant in the larger dbh size classes. The turnover rate of A. sachalinensis was about twice that of the Picea spp. The relative growth rate of understory trees in each species did not differ between different canopy conditions (closed canopy or canopy gap). The competitive advantage between A. sachalinensis and P. glehnii switched as they grew from understory (A. sachalinensis superior competitor) to canopy trees (P. glehnii superior competitor). Meanwhile, A. sachalinensis and P. jezoensis exhibited different environmental preferences. We propose that reversal in competitive superiority between different growth stages and trade-off between longevity and turnover are more important factors to promote their coexistence than regeneration niche differentiation related to canopy gaps in this sub-boreal coniferous forest.
  • Masazumi Tsutsumi, Hisaya Kojima, Shigeru Uemura, Kiyomi Ono, Akihiro Sumida, Toshihiko Hara, Manabu Fukui
    SOIL BIOLOGY & BIOCHEMISTRY 41 2 403 - 408 2009年02月 [査読有り][通常論文]
     
    In a Japanese forest, CH4 uptake rate and methanotrophic community structure in the soil were investigated at four sites of different vegetation. At two of these Sites, undergrowth was dominated by Sasa senanensis, and that of another was dominated by Sasa kurilensis. At the rest site, undergrowth had been removed artificially. The tree-layer composition differed between the two sites with S. senanensis but, tree layer of the other two sites were dominated by the same species. At the site lacking undergrowth, observed CH4 uptake rate was twice as high as that at the other sites. Under laboratory conditions, soil sample from the site lacking undergrowth exhibited CH4 consumption rate higher than that of the adjacent site with the same dominating tree species. The community structures of methanotrophs were investigated with denaturing gradient gel electrophoresis (DGGE) of the gene encoding particulate methane monooxygenase (pmoA). The banding patterns observed were different depending on the type of undergrowth vegetation. The sequences of the DGGE bands were closely related to each other and belonged to the "upland soil cluster alpha" (USC alpha). These results imply possible close relationship between the undergrowth vegetation and methanotrophic communities in forest soils. (c) 2008 Elsevier Ltd. All rights reserved.
  • Akihiro Sumida, Taro Nakai, Masahito Yamada, Kiyomi Ono, Shigeru Uemura, Toshihiko Hara
    SILVA FENNICA 43 5 799 - 816 2009年 [査読有り][通常論文]
     
    We developed a ground-based method for estimating leaf area index (LAI) and vertical distribution of leaf area density (LAD) for two Betula ermanii plots, combining an allometric method for tree leaf area with the MacArthur-Horn (MH) method using a portable laser rangefinder, including a correction for changes in leaf inclination angle along the vertical gradient measured with a portable digital protractor from a canopy access tower in each plot. Vertical distribution of projected leaf area density obtained by the MH method (LAD(MH)) was transformed to relative distribution for allotting fixed LAI to different heights. Hence, we first developed an allometric method for estimating tree leaf area for LAI determination. Trunk cross-sectional area at branching height (AB) was accurately estimated (r(2) = 0.97) from ground-based measurements of tree dimensions. We used this method to apply pipe model allometry between tree leaf area and AB, and estimated LAI (4.56 and 4.57 m(2) m(-2)). We then examined how leaf inclination angle affected estimation of the vertical distribution of actual LAD. Leaf inclination angle measurements revealed that actual LAD in the upper canopy was 1.5-1.8-times higher than LADMH, because of steep leaf inclination, while the correction factor was 1.15-1.25 in the lower canopy. Due to the difference among heights, vertical distribution of LAD estimated with correction for vertical change in leaf inclination was more skewed to the upper canopy than that without correction. We also showed that error in LAD distribution can result if horizontal canopy heterogeneity is neglected when applying the MH method.
  • Takuya Kubo, Tsuyoshi Kobayashi, Kyoko Kato, Seiichi Nishimura, Shigeru Uemura, Kiyomi Ono, Akihiro Sumida, Toshihiko Hara
    AGRICULTURAL AND FOREST METEOROLOGY 148 8-9 1293 - 1304 2008年07月 [査読有り][通常論文]
     
    In order to more accurately evaluate the functional activity of forest stands by canopy production and evapotranspiration, we improved the methods for field measurements and statistical modeling to estimate foliage configuration (spatial distribution of leaves) while simultaneously reconstructing the three-dimensional photosynthetically active photon flux density (PPFD) distribution (PPFD pattern) in a forest canopy. By using a sensor (photodiode) array, a PPFD pattern was observed in summer 2002 under the canopy in an even-aged, pure stand of Japanese mountain birch Betula ermanii Chain. (17-years old) in Hokkaido, northern Japan. A Markov chain Monte Carlo (MCMC) sampling technique is developed such that a set of foliage configurations generated by the model referred to as the Gibbs foliage canopy (GFC) approximates the field-measured PPFD pattern. The posterior distribution of the foliage configurations is generated by the parallel tempering MCMC of eight independent series of foliage configurations. The GFC model generated the posterior distribution of the LAI estimates (mean 4.56) that appeared to be appropriate in comparison to other LAI estimates of the B. ermanii stand based on the indirect and nondestructive methods by LAI-2000 (LAI = 3.43) and litterfall traps (LAI = 5.56) because they could be under- and overestimated, respectively. Our evaluations of the canopy production and evapotranspiration rates suggest that the relationship between LAI and canopy functions was not very simple because it depended on the nonlinear functional forms of the leaf responses of photosynthesis and transpiration to PPFD. The current study demonstrates an application of MCMC techniques that can generate a set of possible structures of unobserved/unobservable objects based on the high-resolution dataset obtained by some indirect (or remote-sensing) methods. (C) 2008 Elsevier B.V. All rights reserved.
  • Ken Ishimaru, Naoki Hirotsu, Takayuki Kashiwagi, Yuka Macloka, Kiyoshi Nagasuga, Kiyorni Ono, Ryu Ohsugi
    PLANT PRODUCTION SCIENCE 11 1 104 - 107 2008年01月 [査読有り][通常論文]
     
    We analyzed the yield characters of field-grown transgenic potato plants (Solanum tuberosum) carrying a maize gene for sucrose-phosphate synthase (SPS), the key enzyme in sucrose synthesis. The SPS activity in the leaves of transgenic plants (line Ag1203) was 2 times that of the control (cv. May Queen). There was no difference in the photosynthetic CO. uptake rates between Ag1203 and May Queen plants, and the leaf starch content of Ag1203 was lower. These observations indicate that the introduction of a foreign SPS gene improved the supply of photosynthate from source (leaves) to sink (tubers). Additionally, leaf senescence of the transgenic potato plants was delayed relative to that of May Queen. The average tuber weight and total yield of Ag1203 plants were at least 20% higher, and the tuber sucrose content, which is related to eating quality, was also higher. Increased translocation of photosynthate and longer period of photosynthetic activity in the leaves may have increased the yield of Ag1203. These results suggest that introduction of the SPS gene improved the yield characters and quality of potato tubers under field conditions.
  • SK Tripathi, A Sumida, K Ono, H Shibata, S Uemura, K Takahashi, T Hara
    ECOLOGICAL RESEARCH 21 2 315 - 320 2006年03月 [査読有り][通常論文]
     
    We investigated the changes in soil microbial biomass C (MBC), microbial biomass N (MBN) and N mineralization in Sasa kurilensis-present (SP) and S. kurilensis-removed (SR) stands in a Betula ermanii forest. The mean levels of MBC and MBN were significantly higher in the SR stand than in the SP, which may have positively influenced the N-mineralization rate as depicted by a significant positive correlation between these variables and the N-mineralization rate. N immobilization and subsequent N release along with decreased use of available soil N due to S. kurilensis removal may have ensured greater N availability in the SR stand.
  • 小野 清美, 石丸 健
    日本作物学会紀事 75 3 241 - 248 2006年 [査読有り][通常論文]
  • SK Tripathi, A Sumida, H Shibata, S Uemura, K Ono, T Hara
    FOREST ECOLOGY AND MANAGEMENT 212 1-3 278 - 290 2005年07月 [査読有り][通常論文]
     
    Dwarf bamboos (mainly Sasa species) form dense undergrowth in many forests in Japan and compete with overstory vegetation for soil resources. For a better understanding of the interaction between tree and undergrowth root for soil nutrients, we compared soil N availability, root biomass and its substrate quality in two Betula ermanii stands. In one stand, the aboveground part of undergrowth (Sasa kurilensis) had been removed for 5 years (SR) and in the other, the undergrowth was present (SP). The SR stand had significantly higher NO3-N levels in soil (1.7-5.5 mg kg(-1) at different soil depths) than the SP stand (1.2-1.7 mg kg(-1)), which may affect the amount and substrate quality of fine root. Although the total (live + dead) of the root and rhizome (root-rh) mass did not differ between the two stands, the total production by these categories was about half in the SR stand than the SP stand. Further, the proportion of dead root-rh mass was about two times in the SR stand than the SP stand. In the investigated depth (to 30 cm), the contribution of the C and N inputs to the total in the uppermost layer (0-10 cm deep) was greater in the SR stand (56-59%) than the SP stand (38-42%). Total root-rh of S. kurilensis in the SP stand contributed to about 38-40% of the total C and N input, and in the SR stand the contribution was still about half that of the SP stand. Further, the acid-insoluble C:N ratio in very fine root and live fine root of B. ermanii was significantly less in the SR stand. The contribution of N content in very fine root to the total root-rh was much greater in the SR (45%) stand than the SP stand (36%). These results suggest that the remaining S. kurilensis detritus and the decreased N use by this species after its removal resulted in increased N availability for B. ermanii. Natural regeneration of tree species after simultaneous death of Sasa species, which is very common in Sasa-dominant forests in Japan, may in part, be facilitated by the presence of residues of the root-rh of Sasa species. (c) 2005 Elsevier B.V. All rights reserved.
  • K Ishimaru, K Ono, T Kashiwagi
    PLANTA 218 3 388 - 395 2004年01月 [査読有り][通常論文]
     
    A gene underlying a quantitative trait locus (QTL) controlling plant height on chromosome 1 (QTLph1) in rice (Oryza sativa L.) was identified using the candidate-gene strategy. First, the function of a targeted gene was analyzed using near isogenic lines (NILs) in which the chromosomal region of a targeted QTL was substituted with that of another line. Second, for physiological information, the candidate gene was selected in the annotation data by the genome sequencing. Physiological analyses of an NIL-expressing QTLph1 (NIL6) suggested that the targeted gene controls plant height by enabling higher amounts of sucrose to be translocated in leaves. The results indicated that the gene for sucrose phosphate synthase (SPS; EC 2.4.1.14), the major limiting enzyme for sucrose synthesis, is a candidate gene for QTLph1 among the annotation results of the region of QTLph1. The higher level of SPS transcripts and the activity of SPS in NIL6 compared to control plants, and the fact that the relative SPS activity per SPS protein content was almost the same between NIL6 and Nipponbare suggested that the higher plant height in NIL6 compared to Nipponbare was due to the high SPS activity in NIL6. In agreement with this hypothesis, transgenic rice plants with a maize SPS gene that had about 3 times the SPS activity of that in Nipponbare (control plants) were significantly taller than Nipponbare from the early growth stage. From these results and the physiological data from NIL6, we concluded that SPS is the targeted gene underlying QTLph1.
  • Kiyomi Ono and Ken Ishimaru, Function and Regulation of Sucrose-phosphate Synthase in Plants
    Kiyomi Ono, Ken Ishimaru
    Recent Research Developments in Plant Science 2 207 - 217 2004年 [査読有り][通常論文]
  • K Ono, H Sasaki, T Hara, K Kobayashi, K Ishimaru
    PLANT PRODUCTION SCIENCE 6 4 281 - 286 2003年12月 [査読有り][通常論文]
     
    To investigate whether increased sucrose-phosphate synthase (SPS) activity alters photosynthetic activity and/or the export of carbon from leaves under elevated CO2 partial pressure ([CO2]), we raised two lines of transgenic rice (H54-9 and H69-7), each overexpressing a maize SPS gene, and wild-type rice under ambient [CO2] (35 Pa) and elevated [CO2] (100 Pa). Under ambient [CO2], no significant difference was observed between the transgenic and wild-type plants in the levels of sucrose or starch in leaves or the photosynthetic activity; but the carbon export rate was higher in H69-7 than in the wild-type. Under elevated [CO2], photosynthetic activity increased in all plants, but the accumulation of starch was significantly repressed in H54-9, whose SPS activity was about 12.5 times higher than that of the wild-type. The carbon export rate was higher in both transgenic lines than the wild-type. We considered that increased SPS activity in rice plants would promote the export of carbon from leaves and, as a result,. starch accumulation in the leaves would be suppressed and/or photosynthetic activity would be promoted under elevated [CO2].
  • N Aoki, K Ono, H Sasaki, SP Seneweera, H Sakai, K Kobayashi, K Ishimaru
    PLANT PRODUCTION SCIENCE 6 1 52 - 58 2003年03月 [査読有り][通常論文]
     
    We monitored the effects of elevated atmospheric CO2 concentrations on the photosynthetic carbon metabolism in the flag leaves of rice plant (Oryza sativa L. cv. Akitakomachi) before and after heading. The plants were grown under ambient (350 ppm : control) or elevated (650 ppm) CO2 conditions. Flag-leaf blades grown under high CO2 accumulated more starch than control leaf blades before heading, but the level of starch declined to almost zero under both CO2 concentrations as soon as the development of ears began. Before heading, the transcript level of sucrose-phosphate synthase (SPS) (EC 2.4.1.14), a key enzyme in the sucrose synthesis in flag-leaf blades was significantly higher under elevated CO2 conditions than under elevated CO2 (P<0.01). The difference in the expression of SPS decreased after heading, coinciding with a change in starch contents in both groups. These results showed that the effects of elevated CO2 concentration on rice plants might vary with the growth stage of the leaf blades. We also discussed the influence of the changes in the carbohydrate metabolism of rice plants caused by elevated CO2 concentration on yield.
  • Ichiro Terashima, Kiyomi Ono
    Plant Cell and Physiology 43 1 70 - 78 2002年 [査読有り][通常論文]
  • K Ishimaru, N Kobayashi, K Ono, M Yano, R Ohsugi
    JOURNAL OF EXPERIMENTAL BOTANY 52 362 1827 - 1833 2001年09月 [査読有り][通常論文]
     
    Genetic relations among the contents of Rubisco, soluble protein and total leaf nitrogen (N) in leaves of rice (Oryza sativa L.) were studied by quantitative trait loci (QTL) analysis with a population of backcross inbred lines (BILs) of japonica Nipponbare x indica Kasalath. The ratio of Rubisco to total leaf N in leaves is the main target in improving photosynthetic N-use efficiency in plants. QTLs controlling Rubisco content were not detected near QTLs for total leaf N content. These results indicate that contents of Rubisco and total leaf N are controlled by different genetics. QTLs that controlled the ratio of Rubisco to total leaf N (CORNs) were detected. These results suggest that some mechanism(s) may be involved in determining this ratio, while the contents of Rubisco and total leaf N are controlled in other ways. In elite BILs, the ratios of Rubisco to total leaf N were higher than those of both parents. These results suggest a good possibility of improving N-use efficiency by CORNs in cultivated rice. A QTL controlling Rubisco content was mapped near a QTL for soluble protein content on chromosome 8 at 5 d after heading and on chromosome 9 at 25 d. In each chromosome region, the peaks of both QTLs overlapped accurately, giving a high possibility of pleiotropic effects by the same genes. Different QTLs controlling soluble protein or Rubisco were detected from those detected at 5 d or 25 d after heading. This suggests that these traits are genetically controlled depending on the growth stages of leaves.
  • K Ishimaru, M Yano, N Aoki, K Ono, T Hirose, SY Lin, L Monna, T Sasaki, R Ohsugi
    THEORETICAL AND APPLIED GENETICS 102 6-7 793 - 800 2001年05月 [査読有り][通常論文]
     
    We have constructed a rice function map by collating the results on quantitative trait loci (QTLs) for 23 important physiological and agronomic characters (including 13 newly measured traits) obtained using backcross inbred lines of japonica Nipponbare x indica Kasalath. Using these materials, The Rice Genome project (RGP) has developed a high-density genetic map. QTLs controlling yield did not overlap with those controlling the morphological and physiological traits supposed to relate to yield, such as photosynthetic ability. This result suggests that these traits do not influence yield, at least in this genetic background and environment. QTLs controlling yield also did not overlap with the structural genes controlling carbon metabolism (rbcS, cytosolic or plastidic fructose-1,6-bisphosphate, R-enzyme, and sucrose synthase). The combination of a function map and results from the RGP can be advantageous. The utility of this map is discussed.
  • K Ono, Y Nishi, A Watanabe, Terashima, I
    PLANT BIOLOGY 3 3 234 - 243 2001年05月 [査読有り][通常論文]
     
    Availability of nitrogen almost always limits plant growth. Therefore, efficient use of nitrogen is essential for the plants. In upright plants, especially when they form dense plant stands, old, lower leaves are shaded by young, upper leaves. Nitrogenous compounds in such shaded leaves are degraded and re-allocated to the developing young, upper leaves. These processes raise efficiency of nitrogen use in photosynthetic production of the plant. For this to occur in the most effective way, leaves would need to sense their photosynthetic status in a plant and increase, maintain or decrease their photosynthetic capacity accordingly. Hypotheses that explain how a leaf can sense its photosynthetic status in the plant are reviewed. They include systems involving phytochrome, sugar-sensing, or cytokinin. Our experimental results with Helianthus annuus and Phaseolus vulgaris plants, which were subject to various shading treatments, are examined in the light of these hypotheses. Our experimental results favoured the sugar-sensing hypothesis: A leaf can sense demand of other plant parts for photosynthates produced by it and nitrogen abundance or deficiency by monitoring its sugar concentration. Problems that are to be challenged in the near future are also pointed out.
  • K Ono, T Hibino, T Kohinata, S Suzuki, Y Tanaka, T Nakamura, T Takabe, T Takabe
    PLANT SCIENCE 160 3 455 - 461 2001年02月 [査読有り][通常論文]
     
    DnaK 1 from a halotolerant cyanobacterium Aphanothece halophyica, was overexpressed in the cytosol of tobacco. When the control and transgenic tobacco seeds were incubated at 27 degreesC, more than 95% of the control and transgenic tobacco seeds germinated. However, at a high incubation temperature, 40 degreesC, only 27% of the control seeds germinated whereas 82% of the transgenic seeds germinated. High temperature treatment during the imbibition of seeds delayed germination more in the control plants than in the transformants although the maximum percentage of germination was similar in both plants. The quantum yields of electron transport and plant elongation were higher in the: transformant during high temperature treatment in young seedlings, but similar in older leaves. DnaK1 was detected in small amounts in seeds and its levels increased during germination. These data indicate that the expression of DnaK1 from a halotolerant cyanobacterium A. halophytica improved the tolerance to high temperature during germination and early growth. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
  • Ken Ishimaru, Tatsuro Hirose, Naohiro Aoki, Sakiko Takahashi, Kiyomi Ono, Shinichi Yamamoto, Jiangzhong Wu, Shoko Saji, Tomoya Baba, Masashi Ugaki, Takashi Yamamoto, Ryu Ohsugi
    Plant Cell and Physiology 42 10 1181 - 1185 2001年 [査読有り][通常論文]
  • S Takahashi, K Ono, M Ugaki, K Ishimaru, N Aoki, R Ohsugi
    PLANT AND CELL PHYSIOLOGY 41 8 977 - 981 2000年08月 [査読有り][通常論文]
     
    To investigate the role of Ser162 in phosphorylation-dependent regulation of maize sucrose-phosphate synthase (SPS) activities in rice, transgenic rice plants expressing wild-type or mutagenized maize SPS were produced. Our results indicate that Ser162 was responsible for overproduction-induced inactivation of SPS protein and for light/dark modulation of this protein in vivo.
  • K Ono, K Ishimaru, N Aoki, R Ohsugi
    PLANT PHYSIOLOGY AND BIOCHEMISTRY 37 12 949 - 953 1999年12月 [査読有り][通常論文]
     
    We investigated whether changes in sucrose-phosphate synthase (EC 2.4.1.14, SPS) activity could alter N remobilization during leaf senescence. Transgenic rice (Oryza sativa L. cv. Nipponbare) with low SPS activities and wild-type rice plants were grown with basal N (1.0 mM NH4NO3) until the late vegetative stage. Subsequently, half of the plants were transferred to a low N (0.1 mM NH4NO3) condition to accelerate leaf senescence, and the others were continuously grown with basal N. With low N supply, the amounts of chlorophyll and soluble protein in flag leaf blades decreased after anthesis in both the low SPS plants and wild-type plants, although the decrease was less in the low SPS plants. Panicle weights were significantly lower in the low SPS plant than in the wild-type plant. These results suggest that the remobilization of N from flag leaves was diminished by suppressing the development of reproductive sinks in the low SPS plant. (C) 1999 Editions scientifiques et medicales Elsevier SAS.
  • Molecular Cloning and Expression Analysis of a Gene for a Sucrose Transporter in Maize (Zea mays L.)
    Naohiro Aoki, Tatsuro Hirose, Sakiko Takahashi, Kiyomi Ono, Ken Ishimaru, Ryu Ohsugi
    Plant Cell Physiology 40 1172 - 1178 1999年 [査読有り][通常論文]
  • Kiyomi Ono, Ken Ishimaru, Naohiro Aoki, Sakiko Takahashi, Kenjiroh Ozawa, Yasunobu Ohkawa, Ryu Ohsugi
    Plant Production Science 2 3 172 - 177 1999年 [査読有り][通常論文]
     
    We obtained transgenic rice (Oryza sativa L. cv. Nipponbare) plants with the gene for maize sucrose-phosphate synthase (EC 2.4.1.14, SPS). Some of the transgenic plants over-expressed maize SPS (over-expressing plants) and some had reduced levels of native SPS protein (co-suppressed plants). There was a positive correlation between the amounts of maize SPS protein and SPS activities. However, apparent Km values for uridine diphosphog-lucose (UDPG) were higher in over-expressing plants than in control rice plants. These results suggest that over-produced maize SPS protein was not fully activated. The sucrose contents did not differ significantly between control and over-expressing rice plants, but they were lower in co-suppressed plants than in control plants. The starch contents were negatively and the sucrose/starch ratios were positively correlated with SPS activities. Thus, carbon partitioning in the transgenic rice was changed, even though rice is predominantly a sucrose-former.
  • K Ono, A Watanabe
    PLANT AND CELL PHYSIOLOGY 38 9 1032 - 1038 1997年09月 [査読有り][通常論文]
     
    Sugar content, transcript levels of rbcS and rbcL, and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) content were measured in the first leaves of sunflower plants grown under high light and low nitrogen supply. After the peak level of the photosynthetic activity was attained, photosynthates accumulated, and the photosynthetic activity decreased. When these plants were supplied with high nitrogen, the sugar content decreased, and the transcript level of rbcS increased. Subsequently, the amount of RuBisCO increased. To decrease the leaf sugar level without changing the nitrogen supply, we applied DCMU, an inhibitor of photosynthesis, to the first leaves of the plants. This treatment also increased the transcript level of rbcS and suppressed the decrease in RuBisCO. These results indicate that sugar repression of rbcS may play a role in the early phases of leaf senescence under nitrogen deficiency.
  • Kiyomi Ono, Ichiro Terashima, Akira Watanabe
    Plant and Cell Physiology 37 8 1083 - 1089 1996年 [査読有り][通常論文]
     
    We examined changes in nitrogen content of the first leaves in relation to growth and nitrogen status of sunflower (Helianthus annuus L.) plants that were raised hydroponically at two irradiance levels (high and low light, HL and LL) and at two nitrogen concentrations (high and low nitrogen, HN and LN). Initial increases in total dry mass and total nitrogen of the whole plant were faster in HL-plants than in LL-plants irrespective of nitrogen supply, but in LN-plants the increase in total nitrogen was soon blunted. When plants grown under the same irradiance were compared, nitrogen content of the first leaves (leaf N) decreased faster in LN-plants than in LN-plants, while for the plants grown at the same nitrogen concentrations, it decreased faster in HL-plants than in LL-plants. Since these changes in leaf N were not explained solely by the changes in plant dry mass or plant nitrogen, we introduced an index, 'nitrogen deficit (ND*)', to quantify nitrogen deficit of the whole plant. ND* was expressed as ND*(t)=[N(max)N(t)] x DM(t), where N(max) and N(t) were nitrogen contents in the young, expanding leaves that had just unfolded to expand, at the initial stage with sufficient nitrogen and at time t, respectively, and DM(t), plant dry mass at t. The decrease in leaf N was expressed as a liner function of ND* irrespective of the growth conditions, which indicates validity of this index. Limitation of the use of ND*, and mechanisms by which leaves sense nitrogen demand are also discussed.
  • K ONO, H HASHIMOTO, S KATOH
    PLANT AND CELL PHYSIOLOGY 36 1 9 - 17 1995年01月 [査読有り][通常論文]
     
    Changes in the number and size of chloroplasts in mesophyll cells were investigated in primary leaves of wheat from full expansion to yellowing under different growth conditions. The number of chloroplasts per cell decreased slowly, although the decrease was steady and statistically significant, until the last stage of leaf senescence, when rapid degradation of chloroplasts took place. Rates of leaf senescence, or the decline in the number of chloroplasts, varied greatly among plants grown at different seasons of the year, but about 20% of chloroplasts always disappeared during the phase when steady loss of chloroplasts occurred. The area of chloroplast disks also decreased gradually but significantly, with a rapid decrease late in senescence. Thus, the total quantity of chloroplasts per mesophyll cell decreased substantially during leaf senescence. Yellowed leaves contained numerous structures that resemble oil drops but no chloroplasts. Decreases in rates of photosynthesis that occur during senescence may, therefore, be largely due to decreases in the quantity of chloroplasts. However, a better correlation was found between the decrease in the maximum capacity for photosynthesis and the degradation of RuBP carboxylase. When plants had been grown with a sufficient supply of nutrients, the number of chloroplasts decreased steadily but at a reduced rate and the reduction in the area of chloroplast disks was strongly suppressed. Thus, the quantitative decrease in chloroplasts in senescing leaves appears to be regulated by the requirements for nutrients (nitrogen) of other part of the plant.

講演・口頭発表等

  • クマイザサの葉の光合成や色素量の3年間の季節変化の比較  [通常講演]
    小野 清美
    第64回日本生態学会 2017年03月 ポスター発表
  • 季節に応じたクマイザサの葉の光合成活性や色素量の変化  [通常講演]
    小野 清美
    第63回日本生態学会 2016年03月 ポスター発表
  • 生育環境の季節変化に対するクマイザサの葉の生理的応答  [通常講演]
    小野 清美
    日本植物学会第78回大会 2014年09月 ポスター発表
  • 雪解け後から上層木展葉までのクマイザサの越冬葉の生理的応答  [通常講演]
    小野 清美
    日本植物学会第77回大会 2013年09月 ポスター発表
  • 異なる生育環境へのダケカンバ苗木の環境応答  [通常講演]
    小野 清美
    第59回日本生態学会大会 2012年03月 ポスター発表
  • 異なる生育環境下でのダケカンバ苗木の葉枚数の変化と個体の成長  [通常講演]
    小野 清美
    第58回日本生態学会大会 2011年03月 ポスター発表
  • 生育光条件の変化に対するダケカンバの葉の窒素量・色素量や展葉数の変化  [通常講演]
    小野 清美
    第57回日本生態学会 2010年03月 口頭発表(一般)
  • 生育条件の変化に対するダケカンバの葉の光合成や展葉数の変化  [通常講演]
    小野清美
    日本植物学会第73回大会 2009年09月 ポスター発表
  • ダケカンバ一年生実生の地上部および地下部の成長や光合成に対する生育温度の影響  [通常講演]
    小野清美, 高山縁, 原登志彦
    第56回日本生態学会大会 2009年03月 ポスター発表
  • 地上部および地下部への温度処理がダケカンバ一年生実生の成長に及ぼす影響  [通常講演]
    小野清美, 高山縁, 原登志彦
    日本植物学会第72回大会 2008年09月 ポスター発表
  • Effects of plant growth and responses to photo-oxidative stress on leaf senescence of Quercus crispula seedlings  [通常講演]
    Kiyomi Ono, Toshihiko Hara
    Phenotypic plasticity in response to environmental changes: Scaling from the molecular to ecosystem levels 2007年10月 ポスター発表
  • 北方林における落葉樹の葉の光ストレス防御応答系の季節変化  [通常講演]
    小野清美, 江藤典子, 内山和子, 渡辺一郎, 来田和人, 原登志彦
    第48回日本植物生理学会年会 2007年03月 ポスター発表
  • 部分被陰処理が葉の老化に与える影響の生育環境による違い  [通常講演]
    小野清美
    第54回日本生態学会 2007年03月 ポスター発表
  • 個体の成長を変える処理が葉の老化に及ぼす影響  [通常講演]
    小野清美
    日本植物学会第70回大会 2006年09月 ポスター発表
  • Effects of plant growth and Photo-oxidative stress on leaf senescence in Quercus crispula seedlings  [通常講演]
    Kiyomi Ono, Toshihiko Hara
    XV Federation of European Societies Plant Biology Congress 2006年07月 ポスター発表
  • 北方林における落葉樹の葉の色素量・窒素量・酵素活性の季節変化  [通常講演]
    小野清美, 江藤典子, 内山和子, 渡辺一郎, 来田和人, 原登志彦
    第53回日本生態学会 2006年03月 ポスター発表
  • 当年生ミズナラ実生の生育条件による葉の老化過程の違い  [通常講演]
    小野清美, 原登志彦
    第47回日本植物生理学会年会 2006年03月 ポスター発表
  • 個体の窒素・炭素バランスや環境ストレスが葉の老化に及ぼす影響  [通常講演]
    小野清美
    第53回日本生態学会 2006年03月 口頭発表(一般)
  • Effects of plant growth and photo-oxidative stress on leaf senescence of Quercus crispula  [通常講演]
    Kiyomi Ono, Toshihiko Hara
    XVII International Botanical Congress 2005年07月 ポスター発表
  • 生育温度・光・窒素供給がミズナラの葉の老化過程に与える影響2  [通常講演]
    小野清美, 原登志彦
    第52回日本生態学会 2005年03月 ポスター発表
  • 生育温度・光・窒素供給がミズナラの葉の老化過程に与える影響  [通常講演]
    小野清美, 江藤典子, 原登志彦
    第51回日本生態学会 2004年08月 ポスター発表
  • Leaf Senescence of Quercus crispula under different growth conditions  [通常講演]
    Kiyomi Ono, Noriko Etoh, Toshihiko Hara
    Plant Biology 2004 2004年07月 ポスター発表
  • 生育環境がミズナラの葉の老化に与える影響  [通常講演]
    小野清美, 江藤典子, 原登志彦
    第45回日本植物生理学会年会 2004年03月 ポスター発表
  • Leaf senescence under different carbon and nitrogen availability  [通常講演]
    Kiyomi Ono
    Plant Biology 2003 2003年03月 ポスター発表
  • ダケカンバ林冠葉の光合成活性などの季節変化―林床のササ除去区と非除去区の比較  [通常講演]
    小野清美, 田畑あずさ, 鯨岡啓輔, 加藤京子, 隅田明洋, 植村滋, 原登志彦
    第50回日本生態学会 2003年03月 ポスター発表
  • 環境に応じた葉の老化とその制御機構  [通常講演]
    小野清美, 寺島一郎
    日本植物学会第65回大会 2001年09月 ポスター発表
  • Possible roles of sugars on leaf senescence  [通常講演]
    Kiyomi Ono, Yukari Nishi, Ichiro Terashima
    12th International Photosynthesis Congress 2001年08月 ポスター発表
  • 葉緑体型グルタミン合成酵素(GS2)遺伝子を導入したイネの強光・高温ストレスとGS2の発現  [通常講演]
    小野清美, 田中義人, 林泰行, 田中章, 高倍鉄子, 高倍昭洋
    第42回日本植物生理学会年会 2001年03月 ポスター発表
  • Effects of the Increase in SPS Activity on Photosynthesis and Carbon Flow in Transgenic Rice Over-expressing a Maize SPS Gene under Elevated CO2  [通常講演]
    Kiyomi Ono, Haruto Sasaki, Ken Ishimaru
    Plant Biology 2000 2000年07月 ポスター発表
  • Changes in Photosynthesis and the Translocation of Photoassimilates in Transgenic Rice Over-expressing a Maize Sucrose-phosphate Synthase Gene under Different CO2 Concentrations  [通常講演]
    Kiyomi Ono, Haruto Sasaki, Ken Ishimaru
    FACE2000 2000年06月 ポスター発表
  • トウモロコシショ糖リン酸合成酵素(SPS)遺伝子を高発現させた形質転換体イネの高CO2条件下における光合成産物の分配、転流の変化  [通常講演]
    小野清美, 石丸健, 佐々木治人
    日本植物学会第63回大会 1999年10月 口頭発表(一般)
  • Changes in the Levels of Soluble Protein and Chlorophyll during Senescence of Flag Leaves, and Yield of Transgenic Rice Plants with a Maize Sucrose-phosphate Synthase Gene  [通常講演]
    Kiyomi Ono, Ken Ishimaru, Ryu Ohsugi
    Internatiuonal Conference of Assimilate Transport and Partitioning 1999年08月 ポスター発表
  • トウモロコシショ糖リン酸合成酵素(SPS)遺伝子を導入した形質転換体イネにおけるSPS活性の変化が乾物重及び収量構成要素に及ぼす影響  [通常講演]
    小野清美, 石丸健, 小沢憲二郎, 大川安信, 大杉立
    日本作物学会第207回講演会 1999年04月 口頭発表(一般)
  • ショ糖リン酸合成酵素(SPS)形質転換体イネにおける止葉の老化、及び収量の変化  [通常講演]
    小野清美, 石丸健, 小沢憲二郎, 大川安信, 大杉立
    日本植物生理学会年会 1999年03月 口頭発表(一般)
  • Effects of a Maize SPS Gene Expressed in Rice on its Activity and Carbon Partitioning  [通常講演]
    Kiyomi Ono, Kaoru Furukawa, Takao Murata, Ken Ishimaru, Kenjiroh Ozawa, Yasunobu Ohkawa, Ryu Ohsugi
    11th International Photosynthesis Congress 1998年08月 ポスター発表
  • トウモロコシSPS遺伝子を導入したイネ止葉のSPSタンパク及び活性の変化  [通常講演]
    小野清美, 古川かおる, 村田孝雄, 石丸健, 小沢憲二郎, 大川安信, 大杉立
    日本植物生理学会年会 1998年05月 口頭発表(一般)
  • 個葉の窒素化合物、炭素化合物の利用過程の被陰処理による変化  [通常講演]
    小野清美, 渡邊昭
    日本植物学会 1996年10月 口頭発表(一般)
  • 異なる窒素供給下でのヒマワリ第一葉の光合成産物量とrbcL, rbcS の発現量の変化  [通常講演]
    小野清美, 渡邊昭
    日本植物生理学会年会 1996年03月 口頭発表(一般)
  • Utilization of Nitrogen and Carbon Sources of Sunflower Leaves under Different Light Conditions and Nitrogen Availability  [通常講演]
    Kiyomi Ono, Akira Watanabe
    10th International Photosynthesis Congress 1995年08月 ポスター発表
  • 異なる光、窒素条件下での個葉の窒素、炭素の利用と生育環境の変化に対する応答  [通常講演]
    小野清美, 渡邊昭
    日本植物生理学会年会 1995年03月 口頭発表(一般)
  • 異なる生育条件における個葉の光合成と窒素化合物及び光合成産物の変化  [通常講演]
    小野清美, 渡邊昭
    日本植物学会 1994年09月 ポスター発表
  • Changes in the Number and Size of Chloroplasts in Senescing Primary Leaves of Wheat  [通常講演]
    Kiyomi Ono, Haruki Hashimoto, Sakae Katoh
    International Botanical Congress 1993年08月 ポスター発表
  • コムギ第一葉における葉緑体の量的変化  [通常講演]
    小野清美, 箸本春樹, 加藤栄
    日本植物学会 1992年09月 口頭発表(一般)

受賞

  • 2010年03月 日本作物学会 第7回日本作物学会論文賞
     Overexpression of a maize SPS gene improves yield characters of potato under field conditions 
    受賞者: Ken Ishiamru;Naoki Hirotsu;Takayuki Kashiwagi;Yuka Madoka;Kiyoshi Nagasuga;Kiyomi Ono;Ryu Ohsugi

共同研究・競争的資金等の研究課題

  • 生育環境が葉の老化に与える影響
    その他の研究制度
  • Leaf senescence under different growth conditions
    The Other Research Programs

教育活動情報

主要な担当授業

  • 植物生態学特論Ⅱ
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 環境科学院
    キーワード : 植物生態学、植物生理学、森林生態学、進化生態学、適応、生活史、成長解析、相対成長関係、頻度分布、光合成、窒素利用、繁殖、個体群動態 plant ecology, forest ecology, evolutionary ecology, adaptation, life history, growth analysis, allometry, frequency distribution, photosynthesis, nitrogen utilization, reproduction, population dynamics
  • 植物生態学特論Ⅰ
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 環境科学院
    キーワード : 生活史, 植物生態学, 植物生理生態学, 森林生態学,生物間相互作用, 群落構造,生物多様性, 生態系, 進化 life-history, plant ecology, plant ecophysiology, forest ecology, biological interaction, community structure, biodiversity, ecosystem, evolution


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