研究者データベース

研究者情報

マスター

アカウント(マスター)

  • 氏名

    片桐 成二(カタギリ セイジ), カタギリ セイジ

所属(マスター)

  • 獣医学研究院 獣医学部門 臨床獣医科学分野

所属(マスター)

  • 獣医学研究院 獣医学部門 臨床獣医科学分野

独自項目

syllabus

  • 2020, 大学院共通授業科目(一般科目):自然科学・応用科学, Inter-Graduate School Classes(General Subject):Natural and Applied Sciences, 修士課程, 大学院共通科目, wildlife medicine, conservation medicine, infectious disease, zoonosis, endangered species
  • 2020, 動物生殖医学特論, Advanced Lecture on Theriogenology, 博士後期課程, 獣医学院
  • 2020, 臨床疾病学特論, Advanced Lecture on Veterinary Clinical Diseases, 博士後期課程, 獣医学院
  • 2020, 総合専門臨床特論, Advanced and Comprehensive Studies on Integrated Specialized Veterinary Clinical Medicine, 博士後期課程, 獣医学院
  • 2020, 野生動物/保全医学特論, Advanced Lecture on Wildlife and Conservation Medicine, 博士後期課程, 獣医学院
  • 2020, 家畜臨床繁殖学, Essential Theriogenology of Domestic Animals, 学士課程, 農学部, 家畜、繁殖、生産性、繁殖技術、繁殖障害、不妊症、繁殖管理
  • 2020, 環境と人間, Environment and People, 学士課程, 全学教育, 日本中央競馬会, 馬、文化、歴史、法律、規制
  • 2020, 繁殖生理学, Reproductive Physiology, 学士課程, 獣医学部, 生殖周期、雌雄生殖器、配偶子、ホルモン、交配、受精、胚の発生、妊娠、分娩、産褥
  • 2020, 産業動物臨床学, Farm Animal Clinical Medicine, 学士課程, 獣医学部, 産業動物、内科、外科、診断、治療
  • 2020, 産業動物獣医療実習Ⅰ, Practice in Farm Animal Medicine I, 学士課程, 獣医学部, 産業動物臨床、生産獣医療、参加型臨床実習
  • 2020, 産業動物獣医療実習Ⅱ, Practice in Farm Animal Medicine II, 学士課程, 獣医学部, 産業動物臨床、生産獣医療、参加型臨床実習

timetable

  • 博士後期課程, 獣医学研究科, 2020, 獣医科学特別研究
  • 博士後期課程, 獣医学研究科, 2020, 獣医科学特論演習
  • 博士後期課程, 獣医学研究科, 2020, 獣医科学基礎科目A 野生動物/保全医学特論
  • 博士後期課程, 獣医学研究科, 2020, 先端獣医科学特論A 動物生殖医学特論
  • 博士後期課程, 獣医学研究科, 2020, 先端獣医科学特論A 国際獣医科学特論:臨床獣医学
  • 博士後期課程, 獣医学院, 2020, 獣医科学特別研究
  • 博士後期課程, 獣医学院, 2020, 獣医科学特論演習
  • 学士課程, 獣医学部, 2020, 繁殖生理学総論
  • 学士課程, 獣医学部, 2020, 繁殖病態学
  • 学士課程, 獣医学部, 2020, 産業動物獣医療実習
  • 学士課程, 獣医学部, 2020, 野生動物学演習
  • 学士課程, 獣医学部, 2020, 研究・臨床セミナー
  • 学士課程, 獣医学部, 2020, アドバンスト演習

PositionHistory

  • 大学院獣医学院副学院長, 2017年4月1日, 2019年3月31日
  • 大学院獣医学院副学院長, 2019年4月1日, 2021年3月31日

researchmap

プロフィール情報

学位

  • Ph.D.(ブリティッシュ・コロンビア大学(カナダ))
  • 獣医学修士(北海道大学)

プロフィール情報

  • 片桐, カタギリ
  • 成二, セイジ
  • ID各種

    200901069361883126

業績リスト

研究キーワード

  • 生殖生理   Reproductive Physiology   

研究分野

  • ライフサイエンス / 獣医学

経歴

  • 2021年04月 - 現在 北海道大学 附属動物病院 病院長
  • 2015年04月 - 現在 北海道大学 大学院獣医学研究院 教授
  • 2017年04月 - 2021年03月 北海道大学 大学院獣医学研究院 副学院長
  • 2009年04月 - 2015年03月 酪農学園大学 獣医学群 獣医学類 教授
  • 1998年10月 - 2009年03月 北海道大学 大学院獣医学研究科 助教授・准教授
  • 1996年04月 - 1996年12月 博士研究員,ブリティッシュ・コロンビア大学,産婦人科内分泌部門 博士研究員

学歴

  • 1991年01月 - 1996年05月   ブリティッシュ・コロンビア大学   Reproductive and Developmental Sciences Program
  • 1985年04月 - 1987年03月   北海道大学   大学院獣医学研究科   獣医学専攻
  • 1981年04月 - 1985年03月   北海道大学   獣医学部

委員歴

  • 1998年 - 現在   日本獣医学会   評議員   日本獣医学会
  • 日本繁殖生物学会   理事   日本繁殖生物学会

論文

MISC

  • 佐藤弘子, KYAW Hay Mar, 柳川洋二郎, 永野昌志, 田上貴祥, 片桐成二 Journal of Reproduction and Development 65 (Suppl Japanese Issue) j86 2019年09月05日 [査読無し][通常論文]
  • BADRAKH Dagvajamts, 柳川洋二郎, 永野昌志, 片桐成二 日本獣医学会学術集会講演要旨集 162nd 442 2019年08月20日 [査読無し][通常論文]
  • 河野光平, 柳川洋二郎, NATTAPONG Ninpetch, 永野昌志, 片桐成二 日本獣医学会学術集会講演要旨集 162nd 441 2019年08月20日 [査読無し][通常論文]
  • 鳥居佳子, 永野昌志, 片桐成二, 柳川洋二郎 日本獣医学会学術集会講演要旨集 162nd 269 2019年08月20日 [査読無し][通常論文]
  • 中島愛理, 菅野智裕, 菅野智裕, 片桐成二, 永野昌志, 柳川洋二郎 日本獣医学会学術集会講演要旨集 162nd 438 2019年08月20日 [査読無し][通常論文]
  • モンゴル国における羊体外受精卵由来産子作出の試み:体外成熟培地への抗酸化剤アスタキサンチンの添加効果
    永野昌志, Purevdorj Erdenetogtokh, 栁川洋二郎, 片桐成二 北海道牛受精卵移植研究会会報 37 19 -19 2018年09月 [査読無し][通常論文]
  • WU Yue, UESHIBA Hiroki, CHEN Zhen, SAKAGUCHI Kenichiro, YANAGAWA Yojiro, KATAGIRI Seiji, NAGANO Masashi, CHIBA Hitoshi, CHIBA Hitoshi, HUI Shu‐Ping JSBMS Letters 43 (Supplement) 105 2018年08月25日 [査読無し][通常論文]
  • 河野光平, 柳川洋二郎, NATTAPONG Ninpetch, 坂口謙一郎, 菅野智裕, 菅野智裕, 鳥居佳子, 植芝滉己, 宮本祥代, 永野昌志, 片桐成二 日本獣医学会学術集会講演要旨集 161st 408 2018年08月21日 [査読無し][通常論文]
  • 菅野智裕, 菅野智裕, 柳川洋二郎, 片桐成二, 永野昌志 日本獣医学会学術集会講演要旨集 161st 411 2018年08月21日 [査読無し][通常論文]
  • 坂口謙一郎, 柳川洋二郎, 吉岡耕治, 須田智子, 植芝滉己, 河野光平, 宮本祥代, 片桐成二, 永野昌志 日本獣医学会学術集会講演要旨集 161st 403 2018年08月21日 [査読無し][通常論文]
  • 植芝滉己, 呉ユエ, 陳震, 坂口謙一郎, 柳川洋二郎, 片桐成二, 千葉仁志, 千葉仁志, 惠淑萍, 永野昌志 日本獣医学会学術集会講演要旨集 161st 405 2018年08月21日 [査読無し][通常論文]
  • 卵胞刺激ホルモンおよび性腺刺激ホルモン放出ホルモン投与による卵巣刺激が河川型水牛 (Bubalus bubalis) における経腟採卵-体外受精成績に及ぼす影響
    坂口謙一郎, Excel Rio S. Maylem, Ramesh C. Tilwani, 栁川洋二郎, 片桐成二, Edwin C. Atabay, Eufrocina P. Atabay, 永野昌志 北海道牛受精卵移植研究会会報 37 18 -18 2018年08月 [査読無し][通常論文]
  • ペレット法により凍結したニホンザル精液に対する融解法の違いが精子性状に与える影響
    栁川洋二郎, 菅野智裕, 兼子明久, 印藤頼子, 佐藤容, 木下こづえ, 今井啓雄, 平井啓久, 片桐成二, 永野昌志, 岡本宗裕 Cryopreservation conference 2017 2017年11月 [査読無し][通常論文]
  • 谷田孝志, 谷田孝志, 坂口謙一郎, 菅野智裕, 池田侑樹, 田嶋彩野, 柳川洋二郎, 永野昌志, 片桐成二 日本獣医学会学術集会講演要旨集 160th 434 2017年08月30日 [査読無し][通常論文]
  • 菅野智裕, 柳川洋二郎, 片桐成二, 永野昌志 日本獣医学会学術集会講演要旨集 160th 432 2017年08月30日 [査読無し][通常論文]
  • 池田侑樹, 奥山みなみ, 奥山みなみ, 森好政晴, 山田未知, 杉浦智親, 柳川洋二郎, 永野昌志, 片桐成二 日本獣医学会学術集会講演要旨集 160th 439 2017年08月30日 [査読無し][通常論文]
  • 鳥居佳子, 菅野智裕, 蓑原悠太朗, 坂元秀行, 松本直也, 冨安洵平, 松井基純, 永野昌志, 片桐成二, 柳川洋二郎 日本獣医学会学術集会講演要旨集 160th 440 2017年08月30日 [査読無し][通常論文]
  • 坂口謙一郎, 谷田孝志, 谷田孝志, 楊応華, 菅野智裕, 永井克尚, 永井克尚, MOHAMED. A. Abdel‐Ghani, 柳川洋二郎, 片桐成二, 永野昌志 日本獣医学会学術集会講演要旨集 160th 432 2017年08月30日 [査読無し][通常論文]
  • 柳川洋二郎, 菅野智裕, 南晶子, 兼子明久, 印藤頼子, 佐藤容, 木下こづえ, 岡本宗裕, 片桐成二, 永野昌志 日本獣医学会学術集会講演要旨集 160th 440 2017年08月30日 [査読無し][通常論文]
  • Astaxanthin improves the growth parameters, developmental competence, and quality of in vitro grown oocytes derived from bovine early antral follicles(和訳中)
    Abdel-Ghani Mohammed.A, 坂口 謙一郎, 菅野 智裕, 柳川 洋二郎, 片桐 成二, 永野 昌志 日本獣医学会学術集会講演要旨集 160回 431 -431 2017年08月 [査読無し][通常論文]
  • Mitochondrial activity and reactive oxygen species(ROS) accumulation in bovine oocytes derived from different culture system for in vitro maturation(和訳中)
    Purevdorj Erdenetogtokh, 柳川 洋二郎, 片桐 成二, 永野 昌志 日本獣医学会学術集会講演要旨集 160回 432 -432 2017年08月 [査読無し][通常論文]
  • 牛卵巣の大きさが顆粒層細胞の性ステロイドホルモン産生能および卵子核成熟能に与える影響
    坂口 謙一郎, 菅野 智裕, 楊 応華, 柳川 洋二郎, 片桐 成二, 永野 昌志 北海道獣医師会雑誌 61 (8) 300 -300 2017年08月 [査読無し][通常論文]
  • 卵胞刺激ホルモンの尾椎硬膜外腔内単回投与が黒毛和種牛の体内・体外胚生産に及ぼす影響
    坂口 謙一郎, 出田 篤司, 土屋 加那美, 真方 文絵, 小牧 春菜, 佐藤 正明, 酒井 伸一, 馬塲 貴大, 柳川 洋二郎, 永野 昌志, 片桐 成二, 小西 正人 北海道獣医師会雑誌 60 (8) 377 -377 2016年08月 [査読無し][通常論文]
  • 北海道内における乳牛への性選別精液使用が後継牛生産と母牛の生存率に与える影響
    菅野 智裕, 萩原 精一, 伊藤 純一, 廣田 和久, 片桐 成二, 永野 昌志 北海道獣医師会雑誌 60 (8) 378 -378 2016年08月 [査読無し][通常論文]
  • 卵胞膜細胞と共培養した牛卵母細胞-顆粒膜細胞複合体の増殖、ステロイド産生とその後の発生能(Growth, steroidogenesis and subsequent developmental competence of bovine oocyte-granulosa cell complex co-cultured with theca cells)
    楊 応華, 菅野 智裕, 坂口 謙一郎, 柳川 洋二郎, 片桐 成二, 永野 昌志 日本獣医学会学術集会講演要旨集 159回 428 -428 2016年08月 [査読無し][通常論文]
  • 牛卵巣内胞状卵胞数と初期胞状卵胞由来卵子顆粒層細胞複合体の性ステロイドホルモン産生能の関係
    坂口 謙一郎, 谷田 孝志, 永井 克尚, 楊 応華, 柳川 洋二郎, 片桐 成二, 永野 昌志 日本獣医学会学術集会講演要旨集 159回 429 -429 2016年08月 [査読無し][通常論文]
  • 牛の小型卵母細胞の発生能に対する成熟前培養時間の影響(Effect of prematuration culture duration on developmental competences of bovine small sized oocytes)
    Mohammed.A. Abdle-Gahni, 坂口 謙一郎, 菅野 智裕, 楊 応華, 柳川 洋二郎, 片桐 成二, 永野 昌志 日本獣医学会学術集会講演要旨集 159回 429 -429 2016年08月 [査読無し][通常論文]
  • 牛精液の射出順序が精子運動性サブポピュレーション構成に与える影響
    菅野 智裕, 柳川 洋二郎, 片桐 成二, 高橋 芳幸, 永野 昌志 日本獣医学会学術集会講演要旨集 159回 430 -430 2016年08月 [査読無し][通常論文]
  • ペレット法による凍結保存が融解後のニホンザル精液の運動性に与える影響
    柳川 洋二郎, 菅野 智裕, 兼子 明久, 印藤 頼子, 岡本 宗裕, 片桐 成二, 永野 昌志 日本獣医学会学術集会講演要旨集 159回 431 -431 2016年08月 [査読無し][通常論文]
  • 萩原 精一, 菅野 智裕, 伊藤 純一, 廣田 和久, 永野 昌志, 片桐 成二 日本獣医師会雑誌 69 (7) 383 -388 2016年07月 [査読無し][通常論文]
     
    性選別乳牛精液が産子の性別、母牛の分娩後生存率に与える影響を明らかにするために道内16農業共済組合により人工授精されたホルスタイン種母牛379,468頭と、その産子の診療履歴を調査した。性選別乳牛精液による雌産子の出生割合(初産牛92.3%、経産牛89.4%)は、通常乳牛精液(初産牛49.1%、経産牛45.4%)より有意に高かった(P<0.01)。性選別乳牛精液により受胎した経産牛の死産発生率(6.1%)は通常乳牛精液で受胎した場合(7.7%)より有意に低かった(P<0.01)。性選別乳牛精液により受胎・分娩した初産・経産牛の分娩後1年生存率は、通常乳牛精液より有意に高かった(P<0.01)。本研究の結果から性選別乳牛精液の利用により、雌産子の出生は増加し、かつ母牛の供用年数は高まることが示唆された。(著者抄録)
  • 萩原 精一, 菅野 智裕, 伊藤 純一, 廣田 和久, 永野 昌志, 片桐 成二 日本獣医師会雑誌 69 (7) 383 -388 2016年07月 [査読無し][通常論文]
     
    性選別乳牛精液が産子の性別、母牛の分娩後生存率に与える影響を明らかにするために道内16農業共済組合により人工授精されたホルスタイン種母牛379,468頭と、その産子の診療履歴を調査した。性選別乳牛精液による雌産子の出生割合(初産牛92.3%、経産牛89.4%)は、通常乳牛精液(初産牛49.1%、経産牛45.4%)より有意に高かった(P<0.01)。性選別乳牛精液により受胎した経産牛の死産発生率(6.1%)は通常乳牛精液で受胎した場合(7.7%)より有意に低かった(P<0.01)。性選別乳牛精液により受胎・分娩した初産・経産牛の分娩後1年生存率は、通常乳牛精液より有意に高かった(P<0.01)。本研究の結果から性選別乳牛精液の利用により、雌産子の出生は増加し、かつ母牛の供用年数は高まることが示唆された。(著者抄録)
  • 高山茉莉, 片桐成二, 片桐成二, 森好政晴, 堂地修, 今井敬 繁殖技術 35 (3) 59 -61 2015年12月 [査読無し][通常論文]
  • 凍結前精液の一時保存方法および冷却方法が融解後のマカク属精子の性状に与える影響
    栁川洋二郎, 杉本幸介, 菅野智裕, 高江州昇, 印藤頼子, 兼子明久, 木下こづえ, 今井啓雄, 岡本宗裕, 片桐成二, 永野昌志 Cryopreservation Conference 2014 2015年10月 [査読無し][通常論文]
  • 牛の卵子品質と卵巣内卵胞数の関係 体内および体外発育卵子を用いた予備的検討
    永井 克尚, 杉山 ちさと, 楊 応華, 柳川 洋二郎, 片桐 成二, 永野 昌志 The Journal of Reproduction and Development 61 (Suppl.) j91 -j91 2015年09月 [査読無し][通常論文]
  • ニホンザルにおける電気刺激時の電圧が精子採取に与える影響および精子凍結保存法の改善について
    杉本 幸介, 柳川 洋二郎, 菅野 智裕, 高江洲 昇, 兼子 明久, 印藤 頼子, 岡本 宗裕, 片桐 成二, 永野 昌志 日本獣医学会学術集会講演要旨集 158回 381 -381 2015年08月 [査読無し][通常論文]
  • Yanagawa Yojiro, Matsuura Yukiko, Suzuki Masatsugu, Katagiri Seiji, Tsubota Toshio Japanese Journal of Veterinary Research 56 (3) 139 -149 2008年11月 [査読無し][通常論文]
     
    Information on steroid hormone receptor distribution in the uterus is essential to understand the roles of their ligands in pregnancy. This study examined the spatio-temporal localization of estrogen receptor alpha (ERα) and progesterone receptor (PR) in the uterus of sika deer (Cervus nippon) to determine the estrogen and progesterone action site during pregnancy. Ovaries and uteri were collected from 21 pregnant sika deer with single fetus and two corpora lutea, ranging from Day 20 to Day 207 of pregnancy. In addition, genital organs were also collected from three sika deer whose gestatio...
  • 古山 敬祐, 片桐 成二, 高橋 芳幸 The Journal of Reproduction and Development 54 (Suppl.) j94 -j94 2008年08月 [査読無し][通常論文]
  • 檜垣彰吾, 岸昌生, 永野昌志, 片桐成二, 高橋芳幸 J Reprod Dev 53 (Supplement) J197 2007年09月25日 [査読無し][通常論文]
  • 岸昌生, 永野昌志, 檜垣彰吾, 片桐成二, 高橋芳幸 日本生殖医学会雑誌 52 (1/2) 37 2007年04月20日 [査読無し][通常論文]
  • ELSHEIKH Adil Salim, TAKAHASHI Yoshiyuki, KATAGIRI Seiji, KANAGAWA Hiroshi Reprod Fert Dev 18 (6) 697 -701 2006年 [査読無し][通常論文]
  • 片桐成二, 奥村嘉子, 永野昌志, 高橋芳幸 日本獣医学会学術集会講演要旨集 140th 152 2005年08月31日 [査読無し][通常論文]
  • 片桐成二, 奥村嘉子, 永野昌志, 高橋芳幸 J Reprod Dev 51 (Supplement) J93 2005年08月25日 [査読無し][通常論文]
  • AAG Adam, Y Takahashi, S Katagiri, M Nagano JOURNAL OF REPRODUCTION AND DEVELOPMENT 50 (5) 579 -586 2004年10月 [査読無し][通常論文]
     
    To develop a reliable follicle culture system, mouse preantral follicles 150-200 am in diameter were cultured individually for 5 or 6 days in membrane inserts or in droplets, and then induced to ovulate with hCG (Experiment 1). The nuclear maturation and developmental competence of the oocytes that ovulated from the follicles cultured in inserts were determined (Experiment 2). There was no significant difference between the two culture systems in the survival rate (83 and 77%). However, follicles cultured in inserts showed a higher ovulation rate (63%) than those cultured in droplets (39%, P < 0.05). About 80% of the oocytes that ovulated from the follicles cultured in inserts were at the metaphase II stage. After in vitro fertilization, 75 and 48% of in vitro ovulated oocytes cleaved and developed into blastocysts, respectively. These results demonstrate that the insert culture system is superior to the droplet culture system in terms of follicular growth and ovulation, and can be used to investigate the growth and ovulation of follicles in vitro.
  • H Hirayama, S Kageyama, S Moriyasu, K Sawai, S Onoe, Y Takahashi, S Katagiri, K Toen, K Watanabe, T Notomi, H Yamashina, S Matsuzaki, A Minamihashi THERIOGENOLOGY 62 (5) 887 -896 2004年09月 [査読無し][通常論文]
     
    Loop-mediated isothermal amplification (LAMP) is a novel DNA amplification method that amplifies a target sequence specifically under isothermal conditions. The product of LAMP is detected by the turbidity of the reaction mixture without electrophoresis. The objective of this study was to develop a rapid sexing method for bovine preimplantation embryos using LAMP. The first experiment was conducted to optimize the DNA extraction method for LAMP-based embryo sexing. The DNA of single blastomeres was extracted using three methods: heat, NaOH, and proteinase K-Tween 20 (PK-TW) treatments. Sexing was performed with two LAMP reactions, male-specific and male-female common reaction, after DNA extraction. The rates of correct determination of sex were 88.9-94.4%, with no difference among methods. The sensitivity and accuracy of LAMP-based embryo sexing were evaluated in the next experiment. The proportion of samples in which the sex was correctly determined was 75-100% for one to five biopsied cells. Lastly, in vivo-derived embryos were examined to verify the usefulness of LAMP-based embryo sexing, and some of these fresh, sexed embryos were transferred into recipient animals. The time needed for sexing was < 1 h. The pregnancy rate was 57.4% and all calves born were of the predicted sex (12 male and 21 female). Therefore, LAMP-based embryo sexing accurately determined gender and is suitable for field application. (c) 2004 Elsevier Inc. All rights reserved.
  • 奥村嘉子, 片桐成二, 永野昌志, 高橋芳幸 J Reprod Dev 50 (Supplement) J81 2004年08月25日 [査読無し][通常論文]
  • AAG Adam, Y Takahashi, S Katagiri, M Nagano JAPANESE JOURNAL OF VETERINARY RESEARCH 52 (2) 77 -84 2004年08月 [査読無し][通常論文]
     
    Effects of oxygen (O-2) tension in the gas atmosphere during in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) on the efficiency of in vitro production of mouse embryos were examined. Mouse oocytes recovered from large antral follicles were subjected to IVM in Waymouth medium for 15, 16 and 17 hr under 5 or 20% O-2 and then subjected to IVF and IVC under 5 or 20% O-2 tension. Lowering the O-2 tension in the gas atmosphere for IVM from 20 to 5% improved the cleavage rate after IVF when the oocytes were subjected to IVM for 15 hr; however, no improvement in the cleavage rate was observed when the culture period for IVM was extended to 16 and 17 hr. Lowering the O-2 tension to 5% for IVM and IVC improved the development of the cleaved oocytes to the blastocyst stage, regardless of the culture period for IVM. However, the O-2 tension for IVF had no remarkable effect on the subsequent embryonic development. These results demonstrate that 5% O-2 is superior to 20% O-2 for IVM and IVC, and suggest that 20% O-2 for IVM may delay oocyte maturation and/or the acquisition of fertilizability and impair the developmental competence of oocytes.
  • 永野昌志, 片桐成二, 滝口満喜, 小松智彦, 永島太郎, 井川真一, 高橋芳幸 北海道獣医師会雑誌 48 (8) 318 2004年08月01日 [査読無し][通常論文]
  • 藤木亮介, 永野昌志, 小西郁男, 穴川明子, 奥村嘉子, 斉藤千寛, 片桐成二, 高橋芳幸 北海道獣医師会雑誌 48 (8) 318 2004年08月01日 [査読無し][通常論文]
  • S Katagiri, Y Takahashi THERIOGENOLOGY 62 (1-2) 103 -112 2004年07月 [査読無し][通常論文]
     
    The objective of the present study was to determine if abnormalities in the cyclic changes of endometrial EGF concentrations can be a diagnostic tool for repeat breeder cows. First, the profile of EGF concentrations during the estrous cycle was determined using endometrial tissues obtained from 31 Holstein cows after slaughter. Cyclic cows had two peaks of EGF concentrations. Then, endometrial tissues were obtained from 12 control and 20 repeat breeder cows by biopsy on Days 3, 7, and 14 of the same estrous cycle. Endometrial EGF concentrations in biopsied samples of the controls were similar to those found in slaughterhouse materials; they were high on Days 3 and 14 (9.2 and 10.4 ng/g tissue, respectively) and low on Day 7 (3.8 ng/g tissue). Concentrations of EGF in repeat breeder cows had a different profile; they were similar on Days 3, 7, and 14 (4.4, 3.4, and 4.0 ng/g tissue, respectively). In conclusion, changes in endometrial EGF concentrations were altered in repeat breeders; these alterations may be a potential diagnostic marker for repeat breeder cows. (C) 2003 Elsevier Inc. All rights reserved.
  • Y Sasamoto, M Sakaguchi, M Nagano, S Katagiri, Y Takahashi JAPANESE JOURNAL OF VETERINARY RESEARCH 51 (3-4) 151 -159 2004年02月 [査読無し][通常論文]
     
    This study aimed to evaluate gonadotropin secretion and the developmental competence of follicular oocytes in dairy cattle during the early postpartum (PP) period. The number of follicles developed after transvaginal ultrasound -guided ovum pick-up (OPU) and fertilizability of retrieved oocytes were compared between cows in which the first dominant follicle (DF) ovulated (ovulated group, n = 4) and did not ovulate (non-ovulated group, n = 3), and between early PP (early PP group, n = 2) and after the resumption of the estrous cycle (cyclic group, n = 2). Follicular ablation was performed 2 - 4 days after the detection of DF in the second follicular wave PP. OPU was repeated 3 - 5 times at 3 or 4 -day intervals from 3 - 4 days after the follicular ablation. At OPU, the follicles were enumerated and all those greater than or equal to5 mm in diameter were aspirated. Recovered oocytes were subjected to in vitro maturation and fertilization. Both criteria were similar between ovulated and non-ovulated groups, and between early PP and cyclic groups. These results suggest that FSH/LH secretions required for follicle recruitment and subsequent follicular growth during the early PP period are similar to those after resumption of the estrous cycle. They also indicate that follicular oocytes during the early PP period have developmental competence.
  • 母体の妊娠認識メカニズムと影響する要因
    酪農ジャーナル 臨時増刊号 2004年 [査読無し][通常論文]
  • EC Atabay, Y Takahashi, S Katagiri, M Nagano, A Koga, Y Kanai THERIOGENOLOGY 61 (1) 15 -23 2004年01月 [査読無し][通常論文]
     
    We determined the efficacy of a microdrop vitrification procedure for cryopreservation of bovine oocytes, using vitrified oocytes as cytoplasts for intraspecies and intergeneric somatic cell nucleus transfer (NT). In vitro matured bovine MII oocytes were vitrified in microdrops with a vitrification solution containing 35% ethylene glycol, 5% polyvinyl pyrrolidone, and 0.4 M trehalose. After warming, approximately 80% of the vitrified oocytes were morphologically normal, and their enucleation rate was similar to that of fresh oocytes. The NT embryos constructed with bovine cumulus cells and the vitrified oocytes developed similar to blastocysts constructed with fresh oocytes, although the cell number of NT blastocysts originating from vitrified oocytes was lower than that of the fresh control. In a second experiment, we examined the development of NT embryos constructed with vitrified bovine oocytes and bovine fibroblasts (intraspecies NT embryos) or swamp buffalo fibroblasts (intergeneric NT embryos). There were no differences between the intraspecies and intergeneric NT embryos in fusion, cleavage and development to blastocysts, except for lower cell numbers in the intergeneric NT blasocysts. In conclusion, the efficacy of this microdrop vitrification procedure and the production of swamp buffalo NT blastocysts using vitrified bovine oocytes was demonstrated. (C) 2003 Elsevier Inc. All rights reserved.
  • S Katagiri, Y Takahashi BIOLOGY OF REPRODUCTION 211 -211 2004年 [査読無し][通常論文]
  • Y Sasamoto, M Sakaguchi, S Katagiri, Y Yamada, Y Takahashi JOURNAL OF VETERINARY MEDICAL SCIENCE 65 (10) 1083 -1086 2003年10月 [査読無し][通常論文]
     
    The effects of twisting and type (single- or double-lumen) of aspiration needle on the efficiency of transvaginal ultrasound-guided ovum pick-up (US-guided OPU) were investigated in cattle. The first study using slaughterhouse ovaries revealed that twisting of the needle during follicle aspiration improved the oocyte recovery rate without deleterious effects on the attachment of cumulus layers. Vacuum pressure affected the oocyte recovery and cumulus attachment, regardless of the needle type. The needle type did not affect the Oocyte recovery or cumulus attachment with an optimized vacuum pressure. In the second study, US-guided OPU was performed in live cows using two types of needles with a vacuum pressure of 75 mmHg. The needle type did not affect the oocyte recovery or cumulus attachment of the recovered oocytes. The results revealed that twisting of the needle is effective in follicle aspiration, and suggested that a single-lumen needle is as useful as a double-lumen needle for US-guided OPU in cattle.
  • MAM Diaz, T Mori, M Nagano, S Katagiri, Y Takahashi J Vet Med Sci 65 (9) 989 -994 2003年09月 [査読無し][通常論文]
     
    The effect of fusion/activation protocol on in vitro development of porcine nuclear transfer (NT) embryos constructed with foreign gene-transfected somatic cells were investigated. NT embryos were produced by using enucleated M 11 oocytes and enhanced green fluorescence protein (EGFP) gene-transfected or non-transfected porcine fetal fibroblasts. One group of NT embryos received a single electrical pulse to induce fusion and activation simultaneously (FAS). The other group was fused 2 hr before activation (FBA) using two kinds of electrical pulses. Electrically activated NT embryos in both groups were treated with cycloheximide (CHX) before culture to assess the development to the blastocyst stage. After 6 days of culture, all morulae and blastocysts derived from EGFP-transfected fibroblasts emitted green fluorescence without mosaicism, and EGFP-gene product was also detected in all morulae and blastocysts examined. NT embryos undergoing FAS showed higher developmental capacity to blastocysts than those undergoing FBA, regardless of the EGFP transfection into the nuclear donor cells. The results also indicated that EGFP-gene transfection into nuclear donor cells has no obvious deleterious effect on the development of NT embryos to blastocysts.
  • EC Atabay, S Katagiri, M Nagano, Y Takahashi JAPANESE JOURNAL OF VETERINARY RESEARCH 50 (4) 185 -194 2003年02月 [査読無し][通常論文]
     
    Effects of recipient oocyte activation methods on the development of nuclear transfer (NT) embryos were investigated. In Exp. 1, cell-cycle phase of serum-starved bovine cumulus cells was examined by flow cytometry. Majority (95.5%) of medium-sized (16-20 mum) cells that made up 56% of total cells was at the G(0)/G(1) phase. NT embryos were constructed by electric fusion with the medium-sized serum-starved cumulus cells and bovine oocytes of 3 different preparations: enucleated oocytes treated with calcium ionophore A 23187 for 5 min and cycloheximide for 5 hr (A 23187/CHX), those treated with ethanol for 7 min and cycloheximide for 2 hr (ethanol/CHX) and those without treatment. In Exp. 2 and 3, developmental competence of NT embryos constructed with A 23187/CHX- and ethanol/CHX-treated oocytes was compared to that of NT embryos constructed with non-treated oocytes, respectively. Further, nuclear behavior in 3 different NT embryos was examined in Exp. 4. Within 1 hr after fusion, majority of the NT embryos constructed with non-treated oocytes showed condensed chromosome. Three hours after fusion, about 50% of NT embryos constructed with non-treated or ethanol/CHX-treated oocytes showed a single pronucleus-like structure. NT embryos constructed with ethanol/CHX-treated oocytes showed similar rates of fusion, cleavage and blastocyst formation to those of the non-treated oocytes. In contrast, NT embryos constructed with A 23187/CHX-treated oocytes did not show any pronucleus-like structure and showed lower cleavage rate and no development to blastocysts. The results indicate that ethanol/CHX-treated oocytes could support development of somatic cell NT embryos to the blastocyst stage at a similar rate to that of non-treated oocytes.
  • A Ishikawa, H Sakamoto, S Katagiri, Y Takahashi JOURNAL OF VETERINARY MEDICAL SCIENCE 65 (1) 99 -102 2003年01月 [査読無し][通常論文]
     
    The longitudinal changes in fecal steroid hormone concentrations and sexual behavior in 2 mated/pregnant and 3 non-mated female Hokkaido brown bears were investigated during the breeding season. Behavioral estrus (standing) lasted for 14 and 32 days in the mated females and for 25 to 36 days in the non-mated females. In non-mated females, sexual behavior, such as female-female mounting and masturbation, was observed for several days before and after the estrous period. In mated females, mean fecal estradiol-17beta concentrations were higher in the estrous period than in the post-estrous period, while fecal progesterone concentrations were higher in the post-estrous period than in the estrous period. The similar trends of steroid hormone changes were observed in the non-mated females.
  • 関塚次郎, 高橋芳幸, 片桐成二, 永野昌志, 坂口実, 山田豊, 中辻浩喜 北海道獣医師会雑誌 46 (8) 239 2002年08月01日 [査読無し][通常論文]
  • A Ishikawa, S Kikuchi, S Katagiri, H Sakamoto, Y Takahashi JAPANESE JOURNAL OF VETERINARY RESEARCH 50 (1) 17 -27 2002年05月 [査読無し][通常論文]
     
    The present study aimed to establish simple systems for measuring fecal steroid hormones in order to monitor the reproductive profiles of captive Hokkaido brown bears. The efficiency of fecal sample processing at the steps of dehydration and extraction and the correlation between steroid concentrations in matched fecal and blood samples were studied. Then, monthly changes in fecal estradiol-17beta and progesterone in female bears, and testosterone in male bears were examined. The procedure was finalized as follows. Fecal samples were dried at 100degreesC for 3 hr and extracted with diethyl ether. The diethyl ether in the extracts was evaporated and residues were reconstituted in ethanol for the assays. Hormone concentrations were quantified using enzyme immunoassays. Concentrations of progesterone and testosterone in fecal and plasma samples were correlated in the systems. The changes in fecal progesterone and testosterone concentrations were similar to those in serum concentrations of bears as reported previously. In contrast, fecal estradiol concentrations did not correlate with plasma levels probably because of the time lag in excretion. However, the changes in estradiol-17beta concentrations in feces in the present study were similar to those reported in serum. In conclusion, fecal progesterone and testosterone assay systems appear practical for monitoring ovarian and testicular activities without immobilization, though methodological improvements and further validation may be required. For the fecal estradiol-17beta assay, there is a need to solve the problem of excretion time lag before the system can be used in the study of reproductive physiology.
  • A Ishikawa, S Kikuchi, S Katagiri, H Sakamoto, Y Takahashi JAPANESE JOURNAL OF VETERINARY RESEARCH 50 (1) 17 -27 2002年05月 [査読無し][通常論文]
     
    The present study aimed to establish simple systems for measuring fecal steroid hormones in order to monitor the reproductive profiles of captive Hokkaido brown bears. The efficiency of fecal sample processing at the steps of dehydration and extraction and the correlation between steroid concentrations in matched fecal and blood samples were studied. Then, monthly changes in fecal estradiol-17beta and progesterone in female bears, and testosterone in male bears were examined. The procedure was finalized as follows. Fecal samples were dried at 100degreesC for 3 hr and extracted with diethyl ether. The diethyl ether in the extracts was evaporated and residues were reconstituted in ethanol for the assays. Hormone concentrations were quantified using enzyme immunoassays. Concentrations of progesterone and testosterone in fecal and plasma samples were correlated in the systems. The changes in fecal progesterone and testosterone concentrations were similar to those in serum concentrations of bears as reported previously. In contrast, fecal estradiol concentrations did not correlate with plasma levels probably because of the time lag in excretion. However, the changes in estradiol-17beta concentrations in feces in the present study were similar to those reported in serum. In conclusion, fecal progesterone and testosterone assay systems appear practical for monitoring ovarian and testicular activities without immobilization, though methodological improvements and further validation may be required. For the fecal estradiol-17beta assay, there is a need to solve the problem of excretion time lag before the system can be used in the study of reproductive physiology.
  • A Ishikawa, M Matsui, H Sakamoto, S Katagiri, Y Takahashi JOURNAL OF VETERINARY MEDICAL SCIENCE 64 (4) 373 -376 2002年04月 [査読無し][通常論文]
     
    Four adult Hokkaido brown bears were used as semen donors, and semen characteristics were examined before freezing and after thawing. A total of 10 electroejaculates were diluted with Tris-egg yolk extender and cooled to 4degreesC over 90 min. Spermatozoa were equilibrated with 4.7% glycerol for 80 min. Semen packed in 0.2-5 ml plastic straws were frozen with liquid nitrogen vapor, Percentages (mean +/- SD) of motile and live sperm were 96 +/- 2 and 86.5 +/- 7.2% before freezing, and 43 +/- 5 and 67.4 +/- 3.9% after thawing, respectively. Although the number of progressively motile sperm after thawing varied among samples (1.8 +/- 1.2 x 10(8) cells/ejaculate), frozen semen in the present study might serve for artificial insemination.
  • EC dela Pena, Y Takahashi, S Katagiri, EC Atabay, M Nagano REPRODUCTION 123 (4) 593 -600 2002年04月 [査読無し][通常論文]
     
    Preantral follicles mechanically isolated from the ovaries of 12-day-old mice were exposed to 2 mol ethylene glycol l(-1) for 2 or 5 min and then to a vitrification solution containing 6 mol ethylene glycol l(-1) and 0.3 mol raffinose l(-1) for 0.5, 1.0 or 2.0 min before vitrification. The vitrified and fresh preantral follicles were treated with collagenase, and the oocyte-granulosa cell complexes (OGCs) obtained were cultured in vitro for 10 days in membrane inserts. Preantral follicles exposed to 2 mol ethylene glycol l(-1) for 5 min and then to the vitrification solution for 0.5 or 1.0 min showed the highest survival rates after warming. The follicular loss after warming was approximately 20%. After in vitro culture, the proportion of viable OGCs from the vitrified follicles was 10% lower than that of the fresh preantral follicles. There were no differences in the rates of maturation, fertilization and subsequent development to blastocysts between the oocytes derived from vitrified follicles and those derived from fresh preantral follicles; however, the developmental competence of the oocytes derived from both vitrified and fresh preantral follicles grown in vitro was lower than that of oocytes grown in vivo. One of the five recipient mice that received 20 blastocysts derived from vitrified preantral follicles gave birth to six live pups. The results of the present study demonstrate for the first time that mouse preantral follicles can be vitrified and that some of the embryos derived from vitrified preantral follicles can develop to live pups.
  • A Ishikawa, M Matsui, H Sakamoto, S Katagiri, Y Takahashi JOURNAL OF VETERINARY MEDICAL SCIENCE 64 (4) 373 -376 2002年04月 [査読無し][通常論文]
     
    Four adult Hokkaido brown bears were used as semen donors, and semen characteristics were examined before freezing and after thawing. A total of 10 electroejaculates were diluted with Tris-egg yolk extender and cooled to 4degreesC over 90 min. Spermatozoa were equilibrated with 4.7% glycerol for 80 min. Semen packed in 0.2-5 ml plastic straws were frozen with liquid nitrogen vapor, Percentages (mean +/- SD) of motile and live sperm were 96 +/- 2 and 86.5 +/- 7.2% before freezing, and 43 +/- 5 and 67.4 +/- 3.9% after thawing, respectively. Although the number of progressively motile sperm after thawing varied among samples (1.8 +/- 1.2 x 10(8) cells/ejaculate), frozen semen in the present study might serve for artificial insemination.
  • EC dela Pena, Y Takahashi, S Katagiri, EC Atabay, M Nagano REPRODUCTION 123 (4) 593 -600 2002年04月 [査読無し][通常論文]
     
    Preantral follicles mechanically isolated from the ovaries of 12-day-old mice were exposed to 2 mol ethylene glycol l(-1) for 2 or 5 min and then to a vitrification solution containing 6 mol ethylene glycol l(-1) and 0.3 mol raffinose l(-1) for 0.5, 1.0 or 2.0 min before vitrification. The vitrified and fresh preantral follicles were treated with collagenase, and the oocyte-granulosa cell complexes (OGCs) obtained were cultured in vitro for 10 days in membrane inserts. Preantral follicles exposed to 2 mol ethylene glycol l(-1) for 5 min and then to the vitrification solution for 0.5 or 1.0 min showed the highest survival rates after warming. The follicular loss after warming was approximately 20%. After in vitro culture, the proportion of viable OGCs from the vitrified follicles was 10% lower than that of the fresh preantral follicles. There were no differences in the rates of maturation, fertilization and subsequent development to blastocysts between the oocytes derived from vitrified follicles and those derived from fresh preantral follicles; however, the developmental competence of the oocytes derived from both vitrified and fresh preantral follicles grown in vitro was lower than that of oocytes grown in vivo. One of the five recipient mice that received 20 blastocysts derived from vitrified preantral follicles gave birth to six live pups. The results of the present study demonstrate for the first time that mouse preantral follicles can be vitrified and that some of the embryos derived from vitrified preantral follicles can develop to live pups.
  • 獣医繁殖学マニュアル
    文永堂出版 59-61 2002年 [査読無し][通常論文]
  • Thatcher WW, Moreira F, Santos J, Mattos R. Novel new concepts for reproductive management and strategies to improve reproductive efficiency in dary cattle(乳牛の新しい繁殖管理法と繁殖効率改善の戦略、訳 片桐成二)
    日本獣医師会雑誌 55:203-207 2002年 [査読無し][通常論文]
  • Soliman M, Ishioka K, Yoshida R, Komabayashi K, Hatai H, Matsui Y, Hirai T, Katagiri S, Takahashi Y, Kawakita Y, Abe H, Kitamura H, Kimura K, Saito M. Serum leptin levels during the periparturient period in cows.
    J Vet Med Sci 64:1053-1056 2002年 [査読無し][通常論文]
  • Kobayashi A, Katagiri S, Kimura T, Ochiai K, Umemura T. Steroid hormones do not reactivate Neospora caninum in ovariectomized mice.
    J Vet Med Sci 64:773-777 2002年 [査読無し][通常論文]
  • Soliman M, Ishioka K, Yoshida R, Komabayashi K, Hatai H, Matsui Y, Hirai T, Katagiri S, Takahashi Y, Kawakita Y, Abe H, Kitamura H, Kimura K, Saito M. Serum leptin levels during the periparturient period in cows.
    J Vet Med Sci 64:1053-1056 2002年 [査読無し][通常論文]
  • Kobayashi A, Katagiri S, Kimura T, Ochiai K, Umemura T. Steroid hormones do not reactivate Neospora caninum in ovariectomized mice.
    J Vet Med Sci 64:773-777 2002年 [査読無し][通常論文]
  • 笹本良彦, 坂口実, 山田豊, 永野昌志, 片桐成二, 高橋芳幸 日本獣医学会学術集会講演要旨集 132nd 228 2001年09月07日 [査読無し][通常論文]
  • 古畑ちひろ, 片桐成二, 高橋芳幸, 永野昌志, 木村和弘, 斉藤昌之 日本不妊学会雑誌 46 (3) 183 2001年07月01日 [査読無し][通常論文]
  • 福井秀樹, 片桐成二, 高橋芳幸, 永野昌志 日本不妊学会雑誌 46 (3) 182-183 2001年07月01日 [査読無し][通常論文]
  • C Bishonga, Y Takahashi, S Katagiri, M Nagano, A Ishikawa JOURNAL OF VETERINARY MEDICAL SCIENCE 63 (6) 619 -624 2001年06月 [査読無し][通常論文]
     
    TWO groups Of mouse preantral follicles with diameters of 125-150 and 151-175 mum were cultured individually for 6 days in a medium supplemented with FSH and fetal calf serum to determine their in vitro growth characteristics. Their oocyte capacity for maturation and development to the blastocyst stage following in vitro fertilization was also assessed. Antral formation rare at the end of culture was higher in the Follicles of 151-175 mum (89%) than 125-150 mum (76%). The timing of antrum formation was different between the two follicle categories: most 151-175 m follicles formed antra earlier than 125-150 mum follicles (days 1 and 5 vs. 5 and 6). However. follicle diameters at the time of antrum formation were the same regardless of the initial size and the culture period. Maturation rates of the oocytes derived from both categories of in vitro grown follicles (70 and 62%) were not different from those of oocytes from in vivo grown follicles (74%). The in vitro derived oocytes, however. showed less cleavage (30 and 35%) than the in vivo derived oocytes(89%). Although the oocytes from both follicle categories developed to the morula stage after in vitro fertilization. blastocysts were only obtained from oocytes derived from the 151-175 mum category. These results demonstrate that an individual follicle culture system using a medium with FSH and fetal calf serum supports in vitro growth of mouse preantral follicles with diameters of 151-175 mum to the preovulatory stage, and that their oocytes have the capability to develop to the blastocyst stage.
  • C Bishonga, Y Takahashi, S Katagiri, M Nagano, A Ishikawa JOURNAL OF VETERINARY MEDICAL SCIENCE 63 (6) 619 -624 2001年06月 [査読無し][通常論文]
     
    TWO groups Of mouse preantral follicles with diameters of 125-150 and 151-175 mum were cultured individually for 6 days in a medium supplemented with FSH and fetal calf serum to determine their in vitro growth characteristics. Their oocyte capacity for maturation and development to the blastocyst stage following in vitro fertilization was also assessed. Antral formation rare at the end of culture was higher in the Follicles of 151-175 mum (89%) than 125-150 mum (76%). The timing of antrum formation was different between the two follicle categories: most 151-175 m follicles formed antra earlier than 125-150 mum follicles (days 1 and 5 vs. 5 and 6). However. follicle diameters at the time of antrum formation were the same regardless of the initial size and the culture period. Maturation rates of the oocytes derived from both categories of in vitro grown follicles (70 and 62%) were not different from those of oocytes from in vivo grown follicles (74%). The in vitro derived oocytes, however. showed less cleavage (30 and 35%) than the in vivo derived oocytes(89%). Although the oocytes from both follicle categories developed to the morula stage after in vitro fertilization. blastocysts were only obtained from oocytes derived from the 151-175 mum category. These results demonstrate that an individual follicle culture system using a medium with FSH and fetal calf serum supports in vitro growth of mouse preantral follicles with diameters of 151-175 mum to the preovulatory stage, and that their oocytes have the capability to develop to the blastocyst stage.
  • EC dela Pena, Y Takahashi, EC Atabay, S Katagiri, M Nagano CRYOBIOLOGY 42 (2) 103 -111 2001年03月 [査読無し][通常論文]
     
    We investigated the effects of preexposure to ethylene glycol (EG) or raffinose on the viability of vitrified mouse oocytes. Ovulated oocytes at the metaphase II stage were preexposed either to 2 M EG for 0, 2, or 5 min or to ascending concentrations (0.15 followed by 0.3 M) of raffinose solution for 2, 5, or 10 min each (here referred to as 2-2,5-5, and 10-10 min, respectively). The oocytes were then exposed to a vitrification solution (VS), 6 M EG + 0.3 M raffinose, for 0.5, 1, 2, or 5 min and then vitrified or immediately diluted. After warming, the developmental capacity of oocytes was determined after irt vitro fertilization. Volume changes in oocytes during preexposures and exposure to the VS were also investigated. The results demonstrated that preexposure to 2 M EG allowed shorter exposure times of oocytes to the VS and that predehydration in raffinose solutions for 5-5, but not 2-2 or 10-10 min, allowed a wider range of exposure times to the VS. Experiments on volume change suggested that the optimum time of exposure to the VS depends on the amount of EG permeation after preexposure to 2 M EG or to raffinose solutions. Preexposures to 2 M EG or raffinose under optimized conditions increased thc viability of vitrified-warmed oocytes compared to direct exposure to VS without preexposures. (C) 2001 Academic Press.
  • EC dela Pena, Y Takahashi, EC Atabay, S Katagiri, M Nagano CRYOBIOLOGY 42 (2) 103 -111 2001年03月 [査読無し][通常論文]
     
    We investigated the effects of preexposure to ethylene glycol (EG) or raffinose on the viability of vitrified mouse oocytes. Ovulated oocytes at the metaphase II stage were preexposed either to 2 M EG for 0, 2, or 5 min or to ascending concentrations (0.15 followed by 0.3 M) of raffinose solution for 2, 5, or 10 min each (here referred to as 2-2,5-5, and 10-10 min, respectively). The oocytes were then exposed to a vitrification solution (VS), 6 M EG + 0.3 M raffinose, for 0.5, 1, 2, or 5 min and then vitrified or immediately diluted. After warming, the developmental capacity of oocytes was determined after irt vitro fertilization. Volume changes in oocytes during preexposures and exposure to the VS were also investigated. The results demonstrated that preexposure to 2 M EG allowed shorter exposure times of oocytes to the VS and that predehydration in raffinose solutions for 5-5, but not 2-2 or 10-10 min, allowed a wider range of exposure times to the VS. Experiments on volume change suggested that the optimum time of exposure to the VS depends on the amount of EG permeation after preexposure to 2 M EG or to raffinose solutions. Preexposures to 2 M EG or raffinose under optimized conditions increased thc viability of vitrified-warmed oocytes compared to direct exposure to VS without preexposures. (C) 2001 Academic Press.
  • C Bishonga, Y Takahashi, S Katagiri, M Nagano, A Ishikawa JAPANESE JOURNAL OF VETERINARY RESEARCH 48 (4) 169 -176 2001年02月 [査読無し][通常論文]
     
    This study examined the relationship among growth, steroid production and transforming growth factor-beta1 (TGF-beta1) immunolocalization in the mouse follicles cultured in vitro to evaluate the hypothesis that normally developing follicles should express TGF-beta1 in the granulosa cells around the time of antrum formation. Preantral follicles with 151-175 mum (large category) and 125 -150 mum (small category) of initial diameters were used as models for normal and retarded follicles, respectively. Growth rate and timing of antrum formation in both categories were comparable to those of in-vivo grown follicles. At the time of antrum formation, follicular diameters were similar between the two follicle categories; however, antral follicles from the large category showed larger number of granulosa cells, higher estradiol production and proportion of follicles with TGF-beta1 positive granulosa cells. Two days after antrum formation, there were no differences in the number of granulosa cells and the proportions of follicles with TGF-beta1 positive granulosa or theca cells between the two categories. Temporal association in large follicles between the increase in estradiol production and proportion of follicles with TGF-beta1 positive granulosa cells at the time of antrum formation supports our hypothesis. Furthermore, this study demonstrated the usefulness of the follicle culture system in the investigations of follicular physiology.
  • Heath SE. 災害発生に伴う動物の救護について (訳 片桐成二)
    北海道獣医師会雑誌 45:15-18 2001年 [査読無し][通常論文]
  • Wolfgang Jochle. End user attitude toward animal welfare and the environmental issues involved(動物福祉とそれに関連する環境問題に対する消費者の姿勢、訳 片桐成二)
    臨床獣医 19:43-47 2001年 [査読無し][通常論文]
  • 片桐成二. 卵子形成と成熟、獣医繁殖学 第2版(森純一、金川弘司、浜名克己 編)
    永堂出版 45-56 2001年 [査読無し][通常論文]
  • 片桐成二. 暑熱ストレス環境下における繁殖成績向上のためのマネージメント
    臨床獣医 19: 30-34 2001年 [査読無し][通常論文]
  • Christopher Bishonga, Yoshiyuki Takahashi, Seiji Katagiri, Masashi Nagano J Reprod Dev 47 (2) 91 -96 2001年 [査読無し][通常論文]
     
    This study determined the immunolocalization of transforming growth factor-β1 (TGF-β1) in the ovarian follicles of the adult mouse at diestrus, proestrus and estrus (10 and 12.5 h after treatment with hCG). The majority (≥72%) of the oocytes were stained positive in type 3b through to type 8 follicles. TGF-β1 staining in the granulosa and theca cells was first observed in type 5b follicles. The percentage of follicles with positively stained granulosa cells increased with follicular development from type 6 to 8 (13 to 82%). The percentage of follicles with positively stained theca cells did not show a clear trend (∼30%) up to 10 h after hCG treatment, but increased in type 7 (79%) and unovulated type 8 (55%) follicles during the ovulation period (12.5 h after hCG). These results indicate that TGF-β1 is involved in follicular development and differentiation.
  • Bishonga C, Takahashi Y, Katagiri S, Nagano M, Ishikawa A. Relationship among growth, steroid production and immunolocalization of transforming growth factor-β1 in the normally developing mouse follicles cultured in vitro.
    Jpn J Vet Res 48: 169-176 2001年 [査読無し][通常論文]
  • Christopher Bishonga, Yoshiyuki Takahashi, Seiji Katagiri, Masashi Nagano Journal of Reproduction and Development 47 (2) 91 -96 2001年 [査読無し][通常論文]
     
    This study determined the immunolocalization of transforming growth factor-β1 (TGF-β1) in the ovarian follicles of the adult mouse at diestrus, proestrus and estrus (10 and 12.5 h after treatment with hCG). The majority (≥72%) of the oocytes were stained positive in type 3b through to type 8 follicles. TGF-β1 staining in the granulosa and theca cells was first observed in type 5b follicles. The percentage of follicles with positively stained granulosa cells increased with follicular development from type 6 to 8 (13 to 82%). The percentage of follicles with positively stained theca cells did not show a clear trend (∼30%) up to 10 h after hCG treatment, but increased in type 7 (79%) and unovulated type 8 (55%) follicles during the ovulation period (12.5 h after hCG). These results indicate that TGF-β1 is involved in follicular development and differentiation.
  • 高橋芳幸, 片桐成二, 永野昌志, NOUR M S M 食肉に関する助成研究調査成果報告書 18 11-15 2000年12月 [査読無し][通常論文]
  • 永野昌志, 高橋芳幸, 片桐成二, 竹下章, 森好政晴, 横田修 北海道獣医師会雑誌 44 (7) 196 2000年07月01日 [査読無し][通常論文]
  • 山下傑夫, 片桐成二, 永野昌志, 高橋芳幸 日本不妊学会雑誌 45 (2) 165 2000年04月01日 [査読無し][通常論文]
  • 山本直人, 永野昌志, 片桐成二, 高橋芳幸 日本不妊学会雑誌 45 (2) 165 2000年04月01日 [査読無し][通常論文]
  • 猪川真紀, 永野昌志, 片桐成二, 高橋芳幸 日本不妊学会雑誌 45 (2) 165 2000年04月01日 [査読無し][通常論文]
  • Cheong HT, Ikeda K, Martinez Diaz MA., Katagiri S and Takahashi Y. Development of reconstituted pig embryos by nuclear transfer of cultured cumulus cells.
    Reprod Fertil Dev 12: 15-20 2000年 [査読無し][通常論文]
  • dela Pena EC、Atabay EC、永野昌志、片桐成二、高橋芳幸. エチレングリコールとラフィノースを用いたマウス卵子のガラス化保存における前処理の効果
    北海道牛受精卵移植研究会会報 19: 31-34 2000年 [査読無し][通常論文]
  • 永野昌志、猪川真紀、山本直人、片桐成二、高橋芳幸. ウシの初期胞状卵胞および一次卵胞の体外培養
    北海道牛受精卵移植研究会会報 19: 8-13 2000年 [査読無し][通常論文]
  • K Ikeda, Y Takahashi, S Katagiri JOURNAL OF VETERINARY MEDICAL SCIENCE 62 (1) 121 -123 2000年01月 [査読無し][通常論文]
     
    Bovine in vitro matured and fertilized oocytes were cultured for 153 hr in groups of 3 or 30 in 30 mu l of modified synthetic oviduct fluid medium supplemented with amino acids. The concentration of ammonium in culture medium at 153 hr of culture was significantly decreased by medium change at 72 hr of culture. However, regardless of embryo density, medium change had no beneficial or detrimental effect on the development of bovine embryos. Increase in the development to blastocysts and production of ammonium were observed when embryos were cultured in groups of 30, These results indicated that the ammonium concentration detected in this culture system has a negligible effect on the development of bovine embryos to blastocysts.
  • Cheong HT, Ikeda K, Martinez Diaz MA., Katagiri S and Takahashi Y. Development of reconstituted pig embryos by nuclear transfer of cultured cumulus cells.
    Reprod Fertil Dev 12: 15-20 2000年 [査読無し][通常論文]
  • K Ikeda, Y Takahashi, S Katagiri JOURNAL OF VETERINARY MEDICAL SCIENCE 62 (1) 121 -123 2000年01月 [査読無し][通常論文]
     
    Bovine in vitro matured and fertilized oocytes were cultured for 153 hr in groups of 3 or 30 in 30 mu l of modified synthetic oviduct fluid medium supplemented with amino acids. The concentration of ammonium in culture medium at 153 hr of culture was significantly decreased by medium change at 72 hr of culture. However, regardless of embryo density, medium change had no beneficial or detrimental effect on the development of bovine embryos. Increase in the development to blastocysts and production of ammonium were observed when embryos were cultured in groups of 30, These results indicated that the ammonium concentration detected in this culture system has a negligible effect on the development of bovine embryos to blastocysts.
  • M Nagano, Y Takahashi, S Katagiri JOURNAL OF VETERINARY MEDICAL SCIENCE 61 (5) 531 -535 1999年05月 [査読無し][通常論文]
     
    In vitro maturation, fertilization and subsequent development of oocytes with homogeneous (category 1), or heterogeneous ooplasm (category 2) were investigated. No significant differences were observed in the nuclear maturation and total fertilization rates between the two categories. However, category 2 oocytes showed a higher normal fertilization rate due to their lower incidence of polyspermy as compared to category 1 oocytes. Electron microscopic study revealed that all category 2 oocytes had cortical granules lined up next to the plasma membrane, and that some category 1 oocytes still had small clusters of cortical granules after maturation. Although the proportion of cleaved zygotes was higher in category 2, the percentages of cleaved zygotes that developed to the blastocyst stage did not differ between the two categories. These results demonstrate that oocytes with heterogeneous ooplasm have a higher capacity for normal fertilization due to the reduction in polyspermy. This can be attributed to the normal distribution of cortical granules in category 2 oocytes after maturation.
  • M Nagano, Y Takahashi, S Katagiri JOURNAL OF VETERINARY MEDICAL SCIENCE 61 (5) 531 -535 1999年05月 [査読無し][通常論文]
     
    In vitro maturation, fertilization and subsequent development of oocytes with homogeneous (category 1), or heterogeneous ooplasm (category 2) were investigated. No significant differences were observed in the nuclear maturation and total fertilization rates between the two categories. However, category 2 oocytes showed a higher normal fertilization rate due to their lower incidence of polyspermy as compared to category 1 oocytes. Electron microscopic study revealed that all category 2 oocytes had cortical granules lined up next to the plasma membrane, and that some category 1 oocytes still had small clusters of cortical granules after maturation. Although the proportion of cleaved zygotes was higher in category 2, the percentages of cleaved zygotes that developed to the blastocyst stage did not differ between the two categories. These results demonstrate that oocytes with heterogeneous ooplasm have a higher capacity for normal fertilization due to the reduction in polyspermy. This can be attributed to the normal distribution of cortical granules in category 2 oocytes after maturation.
  • 笹本良彦、片桐成二、高橋芳幸. ウシの超音波誘導経腟採卵において卵胞吸引時の吸引針の回転と吸引針の違いが卵子回収成績に及ぼす影響
    北海道牛受精卵移植研究会会報 18: 19-22 1999年 [査読無し][通常論文]
  • Masashi Nagano, Yoshiyuki Takahashi, Seiji Katagiri Journal of Reproduction and Development 45 (3) 239 -242 1999年 [査読無し][通常論文]
     
    The efficacy of a water purification system including a high-intensity 185 nm ultra violet lamp with ultrafilter (UV-UF) for eliminating endotoxins was examined. The activity of endotoxins was below the detectable level in UV-UF water. In contrast, ultra-purified water from a system without a UV lamp (UP) showed a high level of endotoxins, despite water resistivity of more than 18 MQ-cm. Bovine IVM/IVF zygotes were cultured for 174 h in protein-free media prepared with UV-UF and UP water. The developmental rate to blastocysts at 150 h was significantly higher in the medium prepared with UV-UF water. The results demonstrate that the UV-UF system is beneficial for eliminating endotoxins from ultra-purified water, and for preparing protein-free media for culture of bovine embryos.
  • Masashi Nagano, Yoshiyuki Takahashi, Seiji Katagiri Journal of Reproduction and Development 45 (3) 239 -242 1999年 [査読無し][通常論文]
     
    The efficacy of a water purification system including a high-intensity 185 nm ultra violet lamp with ultrafilter (UV-UF) for eliminating endotoxins was examined. The activity of endotoxins was below the detectable level in UV-UF water. In contrast, ultra-purified water from a system without a UV lamp (UP) showed a high level of endotoxins, despite water resistivity of more than 18 MQ-cm. Bovine IVM/IVF zygotes were cultured for 174 h in protein-free media prepared with UV-UF and UP water. The developmental rate to blastocysts at 150 h was significantly higher in the medium prepared with UV-UF water. The results demonstrate that the UV-UF system is beneficial for eliminating endotoxins from ultra-purified water, and for preparing protein-free media for culture of bovine embryos.
  • S Katagiri, Y Takahashi, H Kanagawa, BH Yuen, YS Moon JOURNAL OF VETERINARY MEDICAL SCIENCE 60 (7) 791 -794 1998年07月 [査読無し][通常論文]
     
    Objective of this study was to determine the ability of a delayed-implantation-associated protein (MW 170,000, DIAP170K) to inhibit DNA synthesis by mouse blastocysts. Mice were ovariectomized on day 3 of pregnancy and treated with daily injections with I mg progesterone till day 7 to induce delayed implantation. Blastocysts were collected on day 8 with or without a single injection of 25 ng estradiol-17 beta on day 7 that activates blastocyst metabolisms (activated blastocysts and delayed-implanting blastocysts respectively). DNA synthesis was determined by measuring [H-3]thymidine incorportion by blastocysts. DIAP170K at 10 mu g/ml suppressed resumption of DNA synthesis by delayed-implanting blastocysts and suppression was maximal at 50 mu g/ml. However, DIAP170K did not affect DNA synthesis by blastocysts obtained on day 5 of pregnancy (normal blastocysts) and activated blastocysts. Resumption of DNA synthesis in the inner cell mass (ICM) and trophectoderm from delayed-implanting blastocysts was then separately assessed. DNA synthesis resumed in the trophectoderm of intact blastocysts during 24-hr culture but not in the trophectoderm cultured apart from the ICM. DIAP170K inhibited the resumption of DNA synthesis by the trophectoderm of intact delayed-implanting blastocysts but did not affect DNA synthesis by the ICM. In conclusion, DIAP170K inhibits resumption of DNA synthesis by trophectoderm of delayed-implanting blastocysts. This action of DIAP170K may play a central role in maintaining, but not achieving, dormancy of DNA synthesis by delayed-implanting blastocysts in mice.
  • S Katagiri, Y Takahashi, H Kanagawa, BH Yuen, YS Moon JOURNAL OF VETERINARY MEDICAL SCIENCE 60 (7) 791 -794 1998年07月 [査読無し][通常論文]
     
    Objective of this study was to determine the ability of a delayed-implantation-associated protein (MW 170,000, DIAP170K) to inhibit DNA synthesis by mouse blastocysts. Mice were ovariectomized on day 3 of pregnancy and treated with daily injections with I mg progesterone till day 7 to induce delayed implantation. Blastocysts were collected on day 8 with or without a single injection of 25 ng estradiol-17 beta on day 7 that activates blastocyst metabolisms (activated blastocysts and delayed-implanting blastocysts respectively). DNA synthesis was determined by measuring [H-3]thymidine incorportion by blastocysts. DIAP170K at 10 mu g/ml suppressed resumption of DNA synthesis by delayed-implanting blastocysts and suppression was maximal at 50 mu g/ml. However, DIAP170K did not affect DNA synthesis by blastocysts obtained on day 5 of pregnancy (normal blastocysts) and activated blastocysts. Resumption of DNA synthesis in the inner cell mass (ICM) and trophectoderm from delayed-implanting blastocysts was then separately assessed. DNA synthesis resumed in the trophectoderm of intact blastocysts during 24-hr culture but not in the trophectoderm cultured apart from the ICM. DIAP170K inhibited the resumption of DNA synthesis by the trophectoderm of intact delayed-implanting blastocysts but did not affect DNA synthesis by the ICM. In conclusion, DIAP170K inhibits resumption of DNA synthesis by trophectoderm of delayed-implanting blastocysts. This action of DIAP170K may play a central role in maintaining, but not achieving, dormancy of DNA synthesis by delayed-implanting blastocysts in mice.
  • 永野昌志、高橋芳幸、片桐成二、金川弘司. ウシの胞状卵胞から回収した未成熟卵子の超微形態
    北海道牛受精卵移植研究会会報 17: 1-6 1998年 [査読無し][通常論文]
  • Bautista JAN, Dela Pena EC, Katagiri S, Takahashi Y, Kanagawa H. In vitro viability of mouse oocytes vitrified in an ethylene glycol-based solution.
    Jpn J Vet Res 46: 13-18 1998年 [査読無し][通常論文]
  • Elsheikh AS, Takahashi Y, Katagiri S, Kanagawa H. Functional enucleation of mouse metaphase II oocytes with etoposide.
    Jpn J Vet Res 45: 217-220 1998年 [査読無し][通常論文]
  • Seiji Katagiri, Basil H.O. Yuen, Young S. Moon Journal of Reproduction and Development 44 (1) 15 -20 1998年 [査読無し][通常論文]
     
    The present study examined the role for insulin-like growth factor-I (IGF-I) in preimplantation development in rats. The 8-cell stage rat embryos were cultured with 0 (control), 0.02, 0.2, or 2.0 nM human recombinant (hr) IGF-I for 36 h. Morphological development of embryos to the blastocyst stage was stimulated by hrIGF-I at all examined concentrations. Human recombinant IGF-I at ≥0.2 nM increased the number of live cells in the inner cell mass (ICM), the embryonic lineage, of resulting blastocysts by increasing total cell number and decreasing the rate of dead cells in the ICM. Levels of protein synthesis by blastocysts cultured with ≥0.2 nM hrIGF-I increased to the same levels as that of in vivo grown counterparts. When blastocysts were transferred to a receptive uterus, the rates of implantation and fetal development to day 18 of pregnancy in the 0.2 and 2.0 nM hrIGF-I groups were greater than those of the control group. In conclusion, hrIGF-I at ≥ 0.2 nM may promote development of 8-cell rat embryos to blastocysts that are fully-potent to postimplantational development.
  • Bautista JAN, Dela Pena EC, Katagiri S, Takahashi Y, Kanagawa H. In vitro viability of mouse oocytes vitrified in an ethylene glycol-based solution.
    Jpn J Vet Res 46: 13-18 1998年 [査読無し][通常論文]
  • Elsheikh AS, Takahashi Y, Katagiri S, Kanagawa H. Functional enucleation of mouse metaphase II oocytes with etoposide.
    Jpn J Vet Res 45: 217-220 1998年 [査読無し][通常論文]
  • Seiji Katagiri, Basil H.O. Yuen, Young S. Moon Journal of Reproduction and Development 44 (1) 15 -20 1998年 [査読無し][通常論文]
     
    The present study examined the role for insulin-like growth factor-I (IGF-I) in preimplantation development in rats. The 8-cell stage rat embryos were cultured with 0 (control), 0.02, 0.2, or 2.0 nM human recombinant (hr) IGF-I for 36 h. Morphological development of embryos to the blastocyst stage was stimulated by hrIGF-I at all examined concentrations. Human recombinant IGF-I at ≥0.2 nM increased the number of live cells in the inner cell mass (ICM), the embryonic lineage, of resulting blastocysts by increasing total cell number and decreasing the rate of dead cells in the ICM. Levels of protein synthesis by blastocysts cultured with ≥0.2 nM hrIGF-I increased to the same levels as that of in vivo grown counterparts. When blastocysts were transferred to a receptive uterus, the rates of implantation and fetal development to day 18 of pregnancy in the 0.2 and 2.0 nM hrIGF-I groups were greater than those of the control group. In conclusion, hrIGF-I at ≥ 0.2 nM may promote development of 8-cell rat embryos to blastocysts that are fully-potent to postimplantational development.
  • S Katagiri, YS Moon, BH Yuen HUMAN REPRODUCTION 12 (4) 671 -676 1997年04月 [査読無し][通常論文]
     
    This study was designed to determine whether the somatostatin analogue, octreotide, could prevent embryonic loss by normalizing increased uterine insulin-like growth factor-I (IGF-I) action related to hyperoestrogenaemia following superovulation, Superovulated immature and oestradiol-17 beta-treated adult rats were infused with 100 or 300 mu g/ml of octreotide respectively, or injected daily with 1 or 10 mu g of octreotide from day 1 to day 3 of pregnancy. On day 3, embryos were collected from the oviducts and uteri, Uterine luminal fluid was subjected to embryo culture, The amounts of uterine IGF-I and IGF binding proteins (IGFBP) were determined by radioimmunoassay and ligand binding assay respectively, Octreotide infusion normalized uterine IGF-I action following superovulatory and oestradiol-17 beta treatment, by reducing IGF-I concentrations and increasing IGFBP concentrations, Octreotide infusion increased the number of normal embryos by 2.7-fold and 1.7-fold in superovulated and oestradiol-17 beta-treated rats respectively, and reversed the detrimental effects of uterine luminal fluid on embryonic development caused by superovulatory and oestradiol-17 beta treatment, Daily injections with octreotide had similar but reduced effects in all parameters examined in both treatment groups, In conclusion, octreotide may reduce embryonic loss, at least in part, by normalizing IGF-I action following superovulation.
  • S Katagiri, YS Moon, BH Yuen HUMAN REPRODUCTION 12 (4) 671 -676 1997年04月 [査読無し][通常論文]
     
    This study was designed to determine whether the somatostatin analogue, octreotide, could prevent embryonic loss by normalizing increased uterine insulin-like growth factor-I (IGF-I) action related to hyperoestrogenaemia following superovulation, Superovulated immature and oestradiol-17 beta-treated adult rats were infused with 100 or 300 mu g/ml of octreotide respectively, or injected daily with 1 or 10 mu g of octreotide from day 1 to day 3 of pregnancy. On day 3, embryos were collected from the oviducts and uteri, Uterine luminal fluid was subjected to embryo culture, The amounts of uterine IGF-I and IGF binding proteins (IGFBP) were determined by radioimmunoassay and ligand binding assay respectively, Octreotide infusion normalized uterine IGF-I action following superovulatory and oestradiol-17 beta treatment, by reducing IGF-I concentrations and increasing IGFBP concentrations, Octreotide infusion increased the number of normal embryos by 2.7-fold and 1.7-fold in superovulated and oestradiol-17 beta-treated rats respectively, and reversed the detrimental effects of uterine luminal fluid on embryonic development caused by superovulatory and oestradiol-17 beta treatment, Daily injections with octreotide had similar but reduced effects in all parameters examined in both treatment groups, In conclusion, octreotide may reduce embryonic loss, at least in part, by normalizing IGF-I action following superovulation.
  • Mohamed Mohamed Nour、高橋芳幸、片桐成二、金川弘司. ウシ核移植胚の発育に及ぼすレシピエント卵子の品質の影響
    北海道牛受精卵移植研究会会報 16: 40-43 1997年 [査読無し][通常論文]
  • S Katagiri, S Ma, BH Yuen, YS Moon JOURNAL OF REPRODUCTION AND FERTILITY 109 (1) 115 -120 1997年01月 [査読無し][通常論文]
     
    A potential role for insulin-like growth factor I (IGF-I) in the regulation of the uterine electrolyte environment was studied in conjunction with hyperoestrogenaemia caused by superovulation. Uterine luminal fluid from immature rats treated with 4 (control), 10, 20 and 40 iu (superovulation) pregnant mares' serum gonadotrophin (PMSG, day - 2) and the electrolyte composition was determined on day 3 of pregnancy. Superovulation increased total cation content in uterine flushes by more han twofold, suggesting a comparable increase in the uterine luminal fluid volume. Percentages of K+ and HCO3- content to total cations or anions increased by 27% and 16%, respectively, and those of Na+ and Cl- decreased by 26% and 15%, respectively, after superovulation. Daily injections with 1.0 mu g or more oestradiol, from day 0 to day 2, in the 4 iu PMSG-primed immature rats caused similar changes in total cation content and electrolyte composition of uterine luminal fluid. Anti-IGF-I antibody infusion in the superovulated or oestradiol-treated immature rats restored the alterations in cation composition but had no effect on anion composition and total cation content. IGF-I was infused into adult rats to achieve increased IGF-I action observed after superovulation. IGF-I infusion altered electrolyte composition, as is observed after superovulation or oestradiol treatment, but had no effect on total cation content. In conclusion, hyperoestrogenaemia caused by superovulation may alter the uterine electrolyte environment for preimplantation embryonic development. IGF-I appears to play a central role in mediating this action of oestrogen.
  • S Katagiri, S Ma, BH Yuen, YS Moon JOURNAL OF REPRODUCTION AND FERTILITY 109 (1) 115 -120 1997年01月 [査読無し][通常論文]
     
    A potential role for insulin-like growth factor I (IGF-I) in the regulation of the uterine electrolyte environment was studied in conjunction with hyperoestrogenaemia caused by superovulation. Uterine luminal fluid from immature rats treated with 4 (control), 10, 20 and 40 iu (superovulation) pregnant mares' serum gonadotrophin (PMSG, day - 2) and the electrolyte composition was determined on day 3 of pregnancy. Superovulation increased total cation content in uterine flushes by more han twofold, suggesting a comparable increase in the uterine luminal fluid volume. Percentages of K+ and HCO3- content to total cations or anions increased by 27% and 16%, respectively, and those of Na+ and Cl- decreased by 26% and 15%, respectively, after superovulation. Daily injections with 1.0 mu g or more oestradiol, from day 0 to day 2, in the 4 iu PMSG-primed immature rats caused similar changes in total cation content and electrolyte composition of uterine luminal fluid. Anti-IGF-I antibody infusion in the superovulated or oestradiol-treated immature rats restored the alterations in cation composition but had no effect on anion composition and total cation content. IGF-I was infused into adult rats to achieve increased IGF-I action observed after superovulation. IGF-I infusion altered electrolyte composition, as is observed after superovulation or oestradiol treatment, but had no effect on total cation content. In conclusion, hyperoestrogenaemia caused by superovulation may alter the uterine electrolyte environment for preimplantation embryonic development. IGF-I appears to play a central role in mediating this action of oestrogen.
  • S Ma, DK Kalousek, BH Yuen, S Katagiri, YS Moon JOURNAL OF REPRODUCTIVE MEDICINE 41 (6) 379 -383 1996年06月 [査読無し][通常論文]
     
    OBJECTIVE: To develop a method for the cytogenetic evaluation of preimplantation embryos using nonradioactive centromeric probes for chromosomes 1, 16 and X. STUDY DESIGN: The embryos used for this study were either fragmented or polyploid embryos rejected from art in vitro fertilization program. Prior to in situ hybridization, the embryos were treated with 0.5% protease. After application of gradual fixation, conventional hybridization protocol was followed. RESULTS: Ten of 11 embryos showed hybridization signals suggesting that the success rate of in situ hybridization of human embryos is improved when a modified method of digesting the zona pellucida and gradual fixation with removal of the cytoplasm are used. CONCLUSION: The method described in this study demonstrates that the iona pellucida is the key to successful in situ hybridization of whole human embryos. When the zona pellucida is removed, penetration by a probe becomes possible.
  • S Ma, DK Kalousek, BH Yuen, S Katagiri, YS Moon JOURNAL OF REPRODUCTIVE MEDICINE 41 (6) 379 -383 1996年06月 [査読無し][通常論文]
     
    OBJECTIVE: To develop a method for the cytogenetic evaluation of preimplantation embryos using nonradioactive centromeric probes for chromosomes 1, 16 and X. STUDY DESIGN: The embryos used for this study were either fragmented or polyploid embryos rejected from art in vitro fertilization program. Prior to in situ hybridization, the embryos were treated with 0.5% protease. After application of gradual fixation, conventional hybridization protocol was followed. RESULTS: Ten of 11 embryos showed hybridization signals suggesting that the success rate of in situ hybridization of human embryos is improved when a modified method of digesting the zona pellucida and gradual fixation with removal of the cytoplasm are used. CONCLUSION: The method described in this study demonstrates that the iona pellucida is the key to successful in situ hybridization of whole human embryos. When the zona pellucida is removed, penetration by a probe becomes possible.
  • S Katagiri, YS Moon, BH Yuen FERTILITY AND STERILITY 65 (2) 426 -436 1996年02月 [査読無し][通常論文]
     
    Objectives: To examine possible roles of the insulin-like growth factor (IGF) system in increased early embryonic loss after superovulation. Design: Changes in the uterine IGF system were examined in superovulated rats. Insulinlike growth factor I (IGF-I) was infused to the right uterine horns to mimic enhanced IGF-I actions after superovulation. Uterine luminal fluids were collected after IGF-I infusions and embryos were cultured with uterine luminal fluids. Main Outcome Measures: Steroid hormones, IGF-I, IGF binding protein (IGFBP), and IGF-I receptor levels, developmental rate, and cell numbers of embryos. Results: Elevated IGF-I levels and suppressed IGFBP levels were found from days 1 to 3 of pregnancy after superovulation. Uterine luminal fluids of the ICE-I infusion and superovulation groups impaired embryo development in vitro. Anti-IGF-I antibody infusions after superovulation reversed detrimental effects of superovulation. Dialysis of uterine luminal fluids of the IGF-I infusion and superovulation groups before culture improved embryo development. Conclusions: Enhanced IGF-I actions in the uterus after superovulation may be responsible for the increase of early embryonic loss. The detrimental factor for embryo development seems a small molecule and is likely a local product of the uterus in which IGF-I actions are enhanced.
  • S Katagiri, YS Moon, BH Yuen FERTILITY AND STERILITY 65 (2) 426 -436 1996年02月 [査読無し][通常論文]
     
    Objectives: To examine possible roles of the insulin-like growth factor (IGF) system in increased early embryonic loss after superovulation. Design: Changes in the uterine IGF system were examined in superovulated rats. Insulinlike growth factor I (IGF-I) was infused to the right uterine horns to mimic enhanced IGF-I actions after superovulation. Uterine luminal fluids were collected after IGF-I infusions and embryos were cultured with uterine luminal fluids. Main Outcome Measures: Steroid hormones, IGF-I, IGF binding protein (IGFBP), and IGF-I receptor levels, developmental rate, and cell numbers of embryos. Results: Elevated IGF-I levels and suppressed IGFBP levels were found from days 1 to 3 of pregnancy after superovulation. Uterine luminal fluids of the ICE-I infusion and superovulation groups impaired embryo development in vitro. Anti-IGF-I antibody infusions after superovulation reversed detrimental effects of superovulation. Dialysis of uterine luminal fluids of the IGF-I infusion and superovulation groups before culture improved embryo development. Conclusions: Enhanced IGF-I actions in the uterus after superovulation may be responsible for the increase of early embryonic loss. The detrimental factor for embryo development seems a small molecule and is likely a local product of the uterus in which IGF-I actions are enhanced.
  • S KATAGIRI, BH YUEN, YS MOON, Y TAKAHASHI, H KANAGAWA AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY 31 (2-3) 141 -150 1994年03月 [査読無し][通常論文]
     
    PROBLEM: To screen the uterine protein responsible for embryonic dormancy associated with delayed implantation. METHOD: Uterine protein extracts and sera from mice in which delayed implantation had been induced and those from pregnant mice were separated by three steps of chromatogra hy and SDS-PAGE by monitoring an inhibitory activity on trophoblast outgrowth. The presence of the separated protein in the uterine luminal fluid was assessed. Effect of the protein on cell proliferation and RNA synthesis by blastocysts were assessed. RESULTS: A 170-K protein was found in the uterine tissue as well as uterine luminal fluid associated with delayed implantation. The 170-K protein suppressed RNA synthesis by approximately 50% and cell proliferation in blastocysts. CONCLUSION: A 170-K protein is secreted into the uterine lumen during delayed implantation period. The ability of 170-K protein to suppress RNA synthesis and cell proliferation may play a role in regulation of embryonic dormancy associated with delayed implantation.
  • S KATAGIRI, BH YUEN, YS MOON, Y TAKAHASHI, H KANAGAWA AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY 31 (2-3) 141 -150 1994年03月 [査読無し][通常論文]
     
    PROBLEM: To screen the uterine protein responsible for embryonic dormancy associated with delayed implantation. METHOD: Uterine protein extracts and sera from mice in which delayed implantation had been induced and those from pregnant mice were separated by three steps of chromatogra hy and SDS-PAGE by monitoring an inhibitory activity on trophoblast outgrowth. The presence of the separated protein in the uterine luminal fluid was assessed. Effect of the protein on cell proliferation and RNA synthesis by blastocysts were assessed. RESULTS: A 170-K protein was found in the uterine tissue as well as uterine luminal fluid associated with delayed implantation. The 170-K protein suppressed RNA synthesis by approximately 50% and cell proliferation in blastocysts. CONCLUSION: A 170-K protein is secreted into the uterine lumen during delayed implantation period. The ability of 170-K protein to suppress RNA synthesis and cell proliferation may play a role in regulation of embryonic dormancy associated with delayed implantation.
  • Ma S, Kalousek DK, Ho Yuen B, Gomel V, Katagiri S, Moon YS. Chromosome investigation in in vitro fertilization failure.
    J Assis Reprod Genetics 11: 445-451 1994年 [査読無し][通常論文]
  • Ma S, Kalousek DK, Ho Yuen B, Gomel V, Katagiri S, Moon YS. Chromosome investigation in in vitro fertilization failure.
    J Assis Reprod Genetics 11: 445-451 1994年 [査読無し][通常論文]
  • S KATAGIRI, Y TAKAHASHI, M HISHINUMA, H KANAGAWA, O DOCHI, H TAKAKURA JAPANESE JOURNAL OF VETERINARY RESEARCH 39 (1) 11 -21 1991年05月 [査読無し][通常論文]
     
    A sandwich enzymeimmunoassay (EIA) for pregnant mare serum gonadotropin (PMSG) using a microtiter plate was developed. Sensitivity of the assay to PMSG was 15.6 mIU/ml (0.2 ng/well). The PMSG levels in serum were measured with the EIA in superovulated and anti-PMSG rabbit antiserum treated mice and heifers. In mice, the PMSG blood level was measurable in the serum 4-6 days after intraperitoneal injection of 5-30 IU of PMSG. The administration of anti-PMSG antiserum at the same dose level as PMSG caused a rapid decrease in the PMSG blood level, declining to undetectable levels within 17 hours. In heifers, the PMSG level was measurable at 10-11 days after the injection of 2500 or 3000 IU of PMSG. When antiserum was injected 48 hours after the PMSG injection, the clearance rate of PMSG was affected by the route of the administration. The administration of 3000 units of anti-PMSG antiserum intravenously caused a rapid decline and the disappearance of circulating PMSG within 17 hours. When 3000 units of anti-PMSG antiserum was injected intramuscularly, the PMSG blood level also decreased and became unmeasurable 24 hours after administration; however, it was still detectable for up to 17 hours. These results indicate that the administration of anti-PMSG antiserum at the proper timing and dosage could lead to successful superovulation through the improvement of hormonal conditions.
  • S KATAGIRI, Y TAKAHASHI, M HISHINUMA, H KANAGAWA, O DOCHI, H TAKAKURA JAPANESE JOURNAL OF VETERINARY RESEARCH 39 (1) 11 -21 1991年05月 [査読無し][通常論文]
     
    A sandwich enzymeimmunoassay (EIA) for pregnant mare serum gonadotropin (PMSG) using a microtiter plate was developed. Sensitivity of the assay to PMSG was 15.6 mIU/ml (0.2 ng/well). The PMSG levels in serum were measured with the EIA in superovulated and anti-PMSG rabbit antiserum treated mice and heifers. In mice, the PMSG blood level was measurable in the serum 4-6 days after intraperitoneal injection of 5-30 IU of PMSG. The administration of anti-PMSG antiserum at the same dose level as PMSG caused a rapid decrease in the PMSG blood level, declining to undetectable levels within 17 hours. In heifers, the PMSG level was measurable at 10-11 days after the injection of 2500 or 3000 IU of PMSG. When antiserum was injected 48 hours after the PMSG injection, the clearance rate of PMSG was affected by the route of the administration. The administration of 3000 units of anti-PMSG antiserum intravenously caused a rapid decline and the disappearance of circulating PMSG within 17 hours. When 3000 units of anti-PMSG antiserum was injected intramuscularly, the PMSG blood level also decreased and became unmeasurable 24 hours after administration; however, it was still detectable for up to 17 hours. These results indicate that the administration of anti-PMSG antiserum at the proper timing and dosage could lead to successful superovulation through the improvement of hormonal conditions.
  • 片桐成二、首藤文栄、高橋芳幸、金川弘司. ボツリヌス菌産生C1型(C-ST)毒素および酵素(C3)が胚の発育に及ぼす影響
    北海道牛受精卵移植研究会会報 10: 81-84 1991年 [査読無し][通常論文]
  • 菱沼 貢、片桐成二、橘田達慶、西山栄治、高橋芳幸、金川弘司. 犬および猫子宮蓄膿症の発症傾向、臨床症状と血液検査所見について
    北海道獣医師会雑誌 29: 272-276 1985年 [査読無し][通常論文]

所属学協会

  • 日本繁殖生物学会   日本獣医学会   日本生殖医学会   

共同研究・競争的資金等の研究課題

  • 乳牛の不妊症に関する研究
    研究期間 : 1998年
  • Study on infertility in dairy cattle
    研究期間 : 1998年


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