研究者データベース

久保田 晋平(クボタ シンペイ)
遺伝子病制御研究所 病因研究部門
特任講師

基本情報

所属

  • 遺伝子病制御研究所 病因研究部門

職名

  • 特任講師

学位

  • 博士(医学)(2017年03月 東京大学)

J-Global ID

研究キーワード

  • 合成生物学   組織透明化   

研究分野

  • ライフサイエンス / 薬理学
  • ライフサイエンス / 実験病理学
  • ライフサイエンス / システムゲノム科学

職歴

  • 2021年05月 - 現在 北海道大学 遺伝子病制御研究所 特任講師
  • 2020年04月 - 2021年04月 東京大学 医学系研究科分子病理学分野 特任研究員
  • 2017年04月 - 2020年03月 東京大学医学系研究科 分子病理学分野 日本学術振興会 特別研究員
  • 2014年04月 - 2017年03月 東京大学医学系研究科 システムズ薬理学教室 日本学術振興会 特別研究員
  • 2013年04月 - 2014年03月 東京大学医学系研究科 システムズ薬理学教室 博士課程
  • 2007年04月 - 2013年03月 名古屋大学 医学部

研究活動情報

論文

  • Kei Takahashi, Ko Abe, Shimpei I Kubota, Noriaki Fukatsu, Yasuyuki Morishita, Yasuhiro Yoshimatsu, Satoshi Hirakawa, Yoshiaki Kubota, Tetsuro Watabe, Shogo Ehata, Hiroki R Ueda, Teppei Shimamura, Kohei Miyazono
    Nature communications 13 1 5239 - 5239 2022年09月12日 
    The blood and lymphatic vasculature networks are not yet fully understood even in mouse because of the inherent limitations of imaging systems and quantification methods. This study aims to evaluate the usefulness of the tissue-clearing technology for visualizing blood and lymphatic vessels in adult mouse. Clear, unobstructed brain/body imaging cocktails and computational analysis (CUBIC) enables us to capture the high-resolution 3D images of organ- or area-specific vascular structures. To evaluate these 3D structural images, signals are first classified from the original captured images by machine learning at pixel base. Then, these classified target signals are subjected to topological data analysis and non-homogeneous Poisson process model to extract geometric features. Consequently, the structural difference of vasculatures is successfully evaluated in mouse disease models. In conclusion, this study demonstrates the utility of CUBIC for analysis of vascular structures and presents its feasibility as an analysis modality in combination with 3D images and mathematical frameworks.
  • Adam K Glaser, Kevin W Bishop, Lindsey A Barner, Etsuo A Susaki, Shimpei I Kubota, Gan Gao, Robert B Serafin, Pooja Balaram, Emily Turschak, Philip R Nicovich, Hoyin Lai, Luciano A G Lucas, Yating Yi, Eva K Nichols, Hongyi Huang, Nicholas P Reder, Jasmine J Wilson, Ramya Sivakumar, Elya Shamskhou, Caleb R Stoltzfus, Xing Wei, Andrew K Hempton, Marko Pende, Prayag Murawala, Hans-Ulrich Dodt, Takato Imaizumi, Jay Shendure, Brian J Beliveau, Michael Y Gerner, Li Xin, Hu Zhao, Lawrence D True, R Clay Reid, Jayaram Chandrashekar, Hiroki R Ueda, Karel Svoboda, Jonathan T C Liu
    Nature methods 19 613 - 619 2022年05月11日 [査読有り][通常論文]
     
    Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a flexible system that can address imaging applications with varied requirements in terms of resolution, sample size, tissue-clearing protocol, and transparent sample-holder material. Here, we present a 'hybrid' system that combines a unique non-orthogonal dual-objective and conventional (orthogonal) open-top light-sheet (OTLS) architecture for versatile multi-scale volumetric imaging. We demonstrate efficient screening and targeted sub-micrometer imaging of sparse axons within an intact, cleared mouse brain. The same system enables high-throughput automated imaging of multiple specimens, as spotlighted by a quantitative multi-scale analysis of brain metastases. Compared with existing academic and commercial light-sheet microscopy systems, our hybrid OTLS system provides a unique combination of versatility and performance necessary to satisfy the diverse requirements of a growing number of cleared-tissue imaging applications.
  • Kei Takahashi, Ryo Tanabe, Shogo Ehata, Shimpei I Kubota, Yasuyuki Morishita, Hiroki R Ueda, Kohei Miyazono
    Cancer science 112 9 3796 - 3809 2021年06月19日 
    Tissue-clearing technology is an emerging imaging technique currently utilized not only in neuroscience research but also in cancer research. In our previous reports, tissue-clearing methods were used for the detection of metastatic tumors. Here, we showed that the cell cycles of primary and metastatic tumors were visualized by tissue-clearing methods using a reporter system. First, we established cancer cell lines stably expressing fluorescent ubiquitination-based cell cycle indicator (Fucci) reporter with widely used cancer cell lines A549 and 4T1. Fluorescence patterns of the Fucci reporter were investigated in various tumor inoculation models in mice. Interestingly, fluorescence patterns of the Fucci reporter of tumor colonies were different between various organs, and even among colonies in the same organs. The effects of antitumor drugs were also evaluated using these Fucci reporter cells. Of the three antitumor drugs studied, 5-fluorouracil treatment on 4T1-Fucci cells resulted in characteristic fluorescent patterns by the induction of G2 /M arrest both in vitro and in vivo. Thus, the combination of a tissue-clearing method with the Fucci reporter is useful for analyzing the mechanisms of cancer metastasis and drug resistance.
  • Yusaku Momoi, Jun Nishida, Kosuke Miyakuni, Masafumi Kuroda, Shimpei I Kubota, Kohei Miyazono, Shogo Ehata
    Cancer science 112 8 3136 - 3149 2021年06月06日 
    Intratumoral heterogeneity, including in clear cell renal cell carcinoma, is a potential cause of drug resistance and metastatic cancer progression. We specified the heterogeneous population marked by endoglin (also known as CD105) in a preclinical model of clear cell renal cell carcinoma progression. Highly malignant derivatives of human clear cell renal cell carcinoma OS-RC-2 cells were established as OS5Ks by serial orthotopic inoculation in our previous study. Expression of both ENG (encoding endoglin) mRNA and protein were heterogeneously upregulated in OS5Ks, and the endoglin-positive (ENG+ ) population exhibited growth dependency on endoglin in anchorage-independent cultures. Despite the function of endoglin as a type III receptor, transforming growth factor β and bone morphogenetic protein-9 signaling were unlikely to contribute to the proliferative phenotype. Although endoglin has been proposed as a marker for renal cancer-initiating cells, the OS5K-3 ENG+ population did not enrich other reported cancer-initiating cell markers or differentiate into the ENG- population. Mouse tumor inoculation models revealed that the tumor-forming capabilities of OS5K-3 ENG+ and ENG- cells in vivo were highly dependent on the microenvironment, with the renal microenvironment most preferable to ENG+ cells. In conclusion, the renal microenvironment, rather than the hypothesized ENG+ cell-centered hierarchy, maintains cellular heterogeneity in clear cell renal cell carcinoma. Therefore, the effect of the microenvironment should be considered when evaluating the proliferative capability of renal cancer cells in the experimental settings.
  • Shimpei I. Kubota, Kei Takahashi, Tomoyuki Mano, Katsuhiko Matsumoto, Takahiro Katsumata, Shoi Shi, Kazuki Tainaka, Hiroki R. Ueda, Shogo Ehata, Kohei Miyazono
    Communications Biology 4 1 294 - 294 2021年03月 [査読有り]
     
    AbstractTissue clearing is one of the most powerful strategies for a comprehensive analysis of disease progression. Here, we established an integrated pipeline that combines tissue clearing, 3D imaging, and machine learning and applied to a mouse tumour model of experimental lung metastasis using human lung adenocarcinoma A549 cells. This pipeline provided the spatial information of the tumour microenvironment. We further explored the role of transforming growth factor-β (TGF-β) in cancer metastasis. TGF-β-stimulated cancer cells enhanced metastatic colonization of unstimulated-cancer cells in vivo when both cells were mixed. RNA-sequencing analysis showed that expression of the genes related to coagulation and inflammation were up-regulated in TGF-β-stimulated cancer cells. Further, whole-organ analysis revealed accumulation of platelets or macrophages with TGF-β-stimulated cancer cells, suggesting that TGF-β might promote remodelling of the tumour microenvironment, enhancing the colonization of cancer cells. Hence, our integrated pipeline for 3D profiling will help the understanding of the tumour microenvironment.
  • Kei Takahashi, Shimpei I. Kubota, Shogo Ehata, Hiroki R. Ueda, Kohei Miyazono
    STAR Protocols 1 3 100191 - 100191 2020年12月 [査読有り]
  • Shimpei I. Kubota, Kei Takahashi, Jun Nishida, Yasuyuki Morishita, Shogo Ehata, Kazuki Tainaka, Kohei Miyazono, Hiroki R. Ueda
    CELL REPORTS 20 1 236 - 250 2017年07月 [査読有り][通常論文]
     
    Stochastic and proliferative events initiated from a single cell can disrupt homeostatic balance and lead to fatal disease processes such as cancer metastasis. To overcome metastasis, it is necessary to detect and quantify sparsely distributed metastatic cells throughout the body at early stages. Here, we demonstrate that clear, unobstructed brain/ body imaging cocktails and computational analysis (CUBIC)-based cancer (CUBIC-cancer) analysis with a refractive index (RI)=optimized protocol enables comprehensive cancer cell profiling of the whole body and organs. We applied CUBIC-cancer analysis to 13 mouse models using nine cancer cell lines and spatiotemporal quantification of metastatic cancer progression at single-cell resolution. CUBIC-cancer analysis suggests that the epithelial-mesenchymal transition promotes not only extravasation but also cell survival at metastatic sites. CUBIC-cancer analysis is also applicable to pharmacotherapeutic profiling of anti-tumor drugs. CUBIC-cancer analysis is compatible with in vivo bioluminescence imaging and 2D histology. We suggest that a scalable analytical pipeline with these three modalities may contribute to addressing currently incurable metastatic diseases.
  • Kazuki Tainaka, Akihiro Kuno, Shimpei I. Kubota, Tatzya Murakami, Hiroki R. Ueda
    ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 32 32 713 - 741 2016年 [査読有り][通常論文]
     
    Mammalian bodies have more than a billion cells per cubic centimeter, which makes whole-body cell (WBC) profiling of an organism one of the ultimate challenges in biology and medicine. Recent advances in tissue-clearing technology have enabled rapid and comprehensive cellular analyses in whole organs and in the whole body by a combination of state-of-the-art technologies of optical imaging and image informatics. In this review, we focus mainly on the chemical principles in currently available techniques for tissue clearing and staining to facilitate our understanding of their underlying mechanisms. Tissue clearing is usually conducted by the following steps: (a) fixation, (b) permeabilization, (c) decolorizing, and (d) refractive index (RI) matching. To phenotype individual cells after tissue clearing, it is important to visualize genetically encoded fluorescent reporters and/or to stain tissues with fluorescent dyes, fluorescent labeled antibodies, or nucleic acid probes. Although some technical challenges remain, the chemical principles in tissue clearing and staining for WBC profiling will enable various applications, such as identifying cellular circuits across multiple organs and measuring their dynamics in stochastic and proliferative cellular processes, for example, autoimmune and malignant neoplastic diseases.
  • Kazuki Tainaka, Shimpei I. Kubota, Takeru Q. Suyama, Etsuo A. Susaki, Dimitri Perrin, Maki Ukai-Tadenuma, Hideki Ukai, Hiroki R. Ueda
    CELL 159 4 911 - 924 2014年11月 [査読有り][通常論文]
     
    The development of whole-body imaging at single-cell resolution enables system-level approaches to studying cellular circuits in organisms. Previous clearing methods focused on homogenizing mismatched refractive indices of individual tissues, enabling reductions in opacity but falling short of achieving transparency. Here, we show that an aminoalcohol decolorizes blood by efficiently eluting the heme chromophore from hemoglobin. Direct transcardial perfusion of an aminoalcohol-containing cocktail that we previously termed CUBIC coupled with a 10 day to 2 week clearing protocol decolorized and rendered nearly transparent almost all organs of adult mice as well as the entire body of infant and adult mice. This CUBIC-perfusion protocol enables rapid whole-body and whole-organ imaging at single-cell resolution by using light-sheet fluorescent microscopy. The CUBIC protocol is also applicable to 3D pathology, anatomy, and immunohistochemistry of various organs. These results suggest that whole-body imaging of colorless tissues at high resolution will contribute to organism-level systems biology.
  • Keisuke Kuroda, Shinnosuke Yamada, Motoki Tanaka, Michiro Iizuka, Hisashi Yano, Daisuke Mori, Daisuke Tsuboi, Tomoki Nishioka, Takashi Namba, Yukihiko Iizuka, Shimpei Kubota, Taku Nagai, Daisuke Ibi, Rui Wang, Atsushi Enomoto, Mayu Isotani-Sakakibara, Naoya Asai, Kazushi Kimura, Hiroshi Kiyonari, Takaya Abe, Akira Mizoguchi, Masahiro Sokabe, Masahide Takahashi, Kiyofumi Yamada, Kozo Kaibuchi
    HUMAN MOLECULAR GENETICS 20 23 4666 - 4683 2011年12月 [査読有り][通常論文]
     
    Disrupted-In-Schizophrenia 1 (DISC1) is a promising candidate gene for susceptibility to psychiatric disorders, including schizophrenia. DISC1 appears to be involved in neurogenesis, neuronal migration, axon/dendrite formation and synapse formation; during these processes, DISC1 acts as a scaffold protein by interacting with various partners. However, the lack of Disc1 knockout mice and a well-characterized antibody to DISC1 has made it difficult to determine the exact role of DISC1 in vivo. In this study, we generated mice lacking exons 2 and 3 of the Disc1 gene and prepared specific antibodies to the N-and C-termini of DISC1. The Disc1 mutant mice are viable and fertile, and no gross phenotypes, such as disorganization of the brain's cytoarchitecture, were observed. Western blot analysis revealed that the DISC1-specific antibodies recognize a protein with an apparent molecular mass of similar to 100 kDa in brain extracts from wild-type mice but not in brain extracts from DISC1 mutant mice. Immunochemical studies demonstrated that DISC1 is mainly localized to the vicinity of the Golgi apparatus in hippocampal neurons and astrocytes. A deficiency of full-length Disc1 induced a threshold shift in the induction of long-term potentiation in the dentate gyrus. The Disc1 mutant mice displayed abnormal emotional behavior as assessed by the elevated plus-maze and cliff-avoidance tests, thereby suggesting that a deficiency of full-length DISC1 may result in lower anxiety and/or higher impulsivity. Based on these results, we suggest that full-length Disc1-deficient mice and DISC1-specific antibodies are powerful tools for dissecting the pathophysiological functions of DISC1.

講演・口頭発表等

  • 組織透明化技術を用いた三次元病理学解析  [招待講演]
    久保田 晋平
    第111回日本病理学会総会 シンポジウム・ワークショップパネル(指名)
  • 三次元病理解析に資する組織透明化手法の開発  [招待講演]
    Shimpei I. Kubota, Kei Takahashi, Kazuki Tainaka, Shogo Ehata, Hiroki R. Ueda, Kohei Miyazono
    第110回日本病理学会総会 シンポジウム・ワークショップパネル(指名)

その他活動・業績

  • 久保田晋平, 高橋恵生, 田井中一貴, 江幡正悟, 上田泰己, 宮園浩平 日本病理学会会誌 110 (1) 2021年
  • シングルセル解析でなにがわかるか
    竹山 春子, 細川 正人 組織透明化を用いた全身全細胞解析の最前線 2020年07月 [査読無し][通常論文]
  • 久保田晋平, 高橋恵生, 西田純, 江幡正悟, 宮園浩平 日本癌学会学術総会抄録集(Web) 79th 2020年
  • 久保田 晋平, 高橋 恵生, 西田 純, 江幡 正悟, 田井中 一貴, 上田 泰己, 宮園 浩平 日本薬理学会年会要旨集 93 (0) 1 -YIA-16 2020年 [査読無し][通常論文]
     

    It has been recognized that interactions between cancer cells and stroma are important for survival of cancer cells and metastasis at distant organs. In addition, the complexity within tumor microenvironment contributes to resistance of conventional therapy. Therefore, despite decades of cancer researches, it is still difficult to elucidate the complexity of tumor microenvironment at whole organ or whole body level. To understand tumor microenvironment and overcome cancer, it is necessary to detect and quantify sparsely distributed metastatic cells throughout the body or organ at single-cell resolution. 

    Here, we demonstrate that CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis)-based cancer (CUBIC-Cancer) analysis with refractive-indices (RI) optimized protocol enables comprehensive cancer cell profiling in whole body and organs. We applied CUBIC-Cancer analysis to a dozen mouse models using several cancer cells and spatio-temporal quantification of metastatic cancer progression at single-cell resolution. As a result, metastatic foci can be observed and quantified through whole organ or whole body at single-cell resolution. In addition, three-dimensional (3D) monitoring revealed that the patterns of metastasis were dependent upon cancer cell or metastatic organs. Comparing two cancer cell lines, we quantified the difference of metastatic processes between angiogenesis and vessel cooption. Whole-organ profiling with single-cell resolution also enables to quantify the early steps of lung metastasis formation and rejection.CUBIC-Cancer analysis suggests that the epithelial-mesenchymal transition promotes not only extravasation but also cell survival at metastatic sites. CUBIC-Cancer analysis is applicable to pharmacotherapeutic profiling of anti-tumor drugs. CUBIC-Cancer analysis is compatible with in vivo bioluminescence imaging and 2D histology.We suggest that a scalable analytical pipeline with these three modalities may contribute to addressing currently incurable metastatic diseases.

  • TGF-βにより細胞周期が停止した口腔扁平上皮がん細胞の運動能ならびに転移能は亢進する(TGF-β-induced cell cycle arrest is associated with increased migration and metastasis of oral squamous carcinoma cells)
    高橋 和樹, 井上 カタジナアンナ, 戒田 篤志, 高橋 恵生, 久保田 晋平, 須河内 昭成, 内橋 俊大, 田中 晋, 古郷 幹彦, 三浦 雅彦, 宮園 浩平, 渡部 徹郎 日本癌学会総会記事 78回 J -3010 2019年09月 [査読無し][通常論文]
  • 組織透明化を用いたがん微小環境の解析(Visualization of tumor microenvironment using tissue-clearing technology)
    高橋 恵生, 久保田 晋平, 江幡 正悟, 宮園 浩平 日本癌学会総会記事 78回 E -3064 2019年09月 [査読無し][通常論文]
  • 新しいがん治療に向けたがん転移機構の解明 がん微小環境によるがん転移機構の同所性移植モデルを用いた解析(Mechanisms of cancer metastasis for new cancer treatments Analyses of cancer metastasis regulated by tumor microenvironments using orthotopic transplantation models)
    宮園 浩平, 高橋 恵生, 久保田 晋平, 宮國 昂介, 西田 純, 江幡 正悟 日本癌学会総会記事 78回 CS4 -5 2019年09月 [査読無し][通常論文]
  • 組織透明化を用いたがん転移の臓器丸ごとプロファイリング(Whole-organ quantitative analysis of cancer metastasis by tissue clearing)
    久保田 晋平, 高橋 恵生, 西田 純, 江幡 正悟, 宮園 浩平 日本癌学会総会記事 77回 1950 -1950 2018年09月 [査読無し][通常論文]
  • 見る脂質のページ〈透明化による全細胞解析〉組織透明化による全身全細胞解析
    久保田晋平, 高橋恵生, 上田泰己, 宮園浩平 The Lipid 2018年07月 [査読無し][招待有り]
  • 組織透明化技術CUBICを用いる肺がん細胞の転移解析
    高橋恵生, 久保田晋平, 上田泰己, 宮園浩平 Lung Perspectives 26 72 -76 2018年 [査読無し][招待有り]
  • CUBIC-癌分析法を用いた癌転移の全身/全組織的特性解析(Whole-body/organ profiling of cancer metastasis with CUBIC-Cancer analysis)
    久保田 晋平, 高橋 恵生, 西田 純, 江幡 正悟, 田井中 一貴, 上田 泰己, 宮園 浩平 生命科学系学会合同年次大会 2017年度 [2P -1450] 2017年12月 [査読無し][通常論文]
  • CUBIC-癌分析法を用いた癌転移の全身/全組織的特性解析(Whole-body/organ profiling of cancer metastasis with CUBIC-Cancer analysis)
    久保田 晋平, 高橋 恵生, 西田 純, 江幡 正悟, 田井中 一貴, 上田 泰己, 宮園 浩平 生命科学系学会合同年次大会 2017年度 [2P -1450] 2017年12月 [査読無し][通常論文]
  • CUBICによる抗がん剤治療効果の臓器丸ごとプロファイリング
    久保田 晋平, 高橋 恵生, 西田 純, 江幡 正悟, 宮園 浩平 日本癌学会総会記事 76回 J -1041 2017年09月 [査読無し][通常論文]
  • CUBICによる臓器透明化を用いたがん微小転移の観察
    高橋 恵生, 久保田 晋平, 西田 純, 江幡 正悟, 宮園 浩平 日本癌学会総会記事 76回 J -3097 2017年09月 [査読無し][通常論文]
  • 癌微小転移の臓器まるごと病理学的解析
    久保田 晋平, 高橋 恵生, 西田 純, 江幡 正悟, 宮園 浩平 日本癌学会総会記事 75回 J -3054 2016年10月 [査読無し][通常論文]
  • タンパク質可視化・制御の新技術 細胞局所から個体まで CUBIC 化学混合液を用いた単一細胞レベルでの全器官と全組織のイメージング法(CUBIC: whole-organ, whole-body imaging with single-cell resolution using chemical cocktails)
    田井中 一貴, 洲崎 悦生, 久保田 晋平, 上田 泰己 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集 88回・38回 [2S4 -5] 2015年12月 [査読無し][通常論文]
  • 一細胞解像度個体全身三次元イメージング
    久保田晋平, 田井中一貴, 上田泰己 細胞工学 34 278 -282 2015年03月 [査読無し][招待有り]
  • 久保田 晋平, 田井中 一貴, 上田 泰己 細胞工学 34 (3) 278 -282 2015年 [査読無し][通常論文]
  • 久保田晋平, 田井中一貴, 上田泰己 日本薬理学会関東部会プログラム・要旨集 131st 67 2014年 [査読無し][通常論文]
  • 田井中一貴, 久保田晋平, 上田泰己 日本薬理学会関東部会プログラム・要旨集 131st 66 2014年 [査読無し][通常論文]
  • 久保田晋平, 森大輔, 黒田啓介, 坪井大輔, 貝淵弘三 生化学 ROMBUNNO.2P-0958 2010年 [査読無し][通常論文]

受賞

  • 2021年10月 一般財団法人 エヌエフ基金 2021年度 研究開発奨励賞
     組織透明化手法の開発による神経-免疫連関機構の計測
  • 2019年03月 花王芸術・科学財団 平成31年度花王科学奨励賞
     
    受賞者: 久保田 晋平
  • 2018年05月 第6回 後藤喜代子・ポールブルダリ科学賞 特別賞
     
    受賞者: 久保田 晋平
  • 2018年03月 細胞ダイバース第一回若手ワークショップ 最優秀発表賞
     
    受賞者: 久保田 晋平
  • 2018年02月 第34回 井上研究奨励賞
     
    受賞者: 久保田 晋平
  • 2017年06月 第31回 独創性を拓く 先端技術大賞受賞 (フジテレビジョン賞)
     
    受賞者: 久保田 晋平
  • 2017年01月 第7回 日本学術振興会育志賞
     
    受賞者: 久保田 晋平
  • 2016年10月 平成28年度 Tadamitsu Kishimoto International Travel Award
     
    受賞者: 久保田 晋平
  • 2016年09月 QBiC Symposium travel fellowship
     
    受賞者: 久保田 晋平
  • 2015年03月 日本薬理学会 第88回日本薬理学会年会優秀発表者賞
     
    受賞者: 久保田 晋平
  • 2014年09月 International symposium "Homeodynamics in Clocks, Sleep and Metabolism" Tokyo Translational Therapeutics Meeting Best Poster Award
     
    受賞者: 久保田 晋平

共同研究・競争的資金等の研究課題

  • 全身全細胞解析技術を用いた微小炎症制御機構の解明
    公益財団法人 日本応用酵素協会:2022 年度 成人病の病因・病態の解明に関する研究助成(TMFC)
    研究期間 : 2022年
  • 治療抵抗性獲得機序の解明に資する全身がん病態可視化技術の開発
    国際科学技術財団:
    研究期間 : 2018年04月 -2019年03月 
    代表者 : 久保田 晋平


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