研究者データベース

岡嶋 孝治(オカジマ タカハル)
情報科学研究院 生命人間情報科学部門 バイオエンジニアリング分野
教授

基本情報

所属

  • 情報科学研究院 生命人間情報科学部門 バイオエンジニアリング分野

職名

  • 教授

学位

  • 博士(理学)(東京工業大学)

ホームページURL

J-Global ID

研究キーワード

  • 応用物理学一般   生物物理学   バイオナノテクノロジー   Polymer Science   Biophysics   Bionanotechnology   

研究分野

  • ライフサイエンス / 生物物理学
  • ナノテク・材料 / 応用物理一般
  • 自然科学一般 / 生物物理、化学物理、ソフトマターの物理
  • ナノテク・材料 / ナノバイオサイエンス
  • ナノテク・材料 / ナノ材料科学

職歴

  • 2019年04月 - 現在 北海道大学 大学院情報科学研究院 教授
  • 2013年05月 - 2019年03月 北海道大学 大学院情報科学研究科 教授
  • 2007年04月 - 2013年04月 北海道大学 大学院情報科学研究科 助教授
  • 2003年04月 - 2007年03月 北海道大学電子科学研究所附属ナノテクノロジー研究センター 助教授
  • 1998年04月 - 2003年03月 東京工業大学生命理工学研究科 助手
  • 1995年10月 - 1998年03月 東京工業大学 生命理工学部・生体機構学科 助手

学歴

  •         - 1993年   東京工業大学   理工学研究科   物理学専攻
  •         - 1991年   横浜市立大学   文理学部   理科(物理学)

所属学協会

  • American Society for Cell Biology   表面科学会   日本生物物理学会   応用物理学会   Biophysical Society(米国)   

研究活動情報

論文

  • Y. Fujii, W. C. Koizumi, T. Imai, M. Yokobori, T. Matsuo, K. Oka, K. Hotta, T. Okajima
    Communications Biology 4 1 2021年 [査読有り]
     
    AbstractDuring the developmental processes of embryos, cells undergo massive deformation and division that are regulated by mechanical cues. However, little is known about how embryonic cells change their mechanical properties during different cleavage stages. Here, using atomic force microscopy, we investigated the stiffness of cells in ascidian embryos from the fertilised egg to the stage before gastrulation. In both animal and vegetal hemispheres, we observed a Rho kinase (ROCK)-independent cell stiffening that the cell stiffness exhibited a remarkable increase at the timing of cell division where cortical actin filaments were organized. Furthermore, in the vegetal hemisphere, we observed another mechanical behaviour, i.e., a ROCK-associated cell stiffening, which was retained even after cell division or occurred without division and propagated sequentially toward adjacent cells, displaying a characteristic cell-to-cell mechanical variation. The results indicate that the mechanical properties of embryonic cells are regulated at the single cell level in different germ layers.
  • Measuring viscoelasticity of soft biological samples using atomic force microscopy
    Yuri M. Efremov, Takaharu Okajima, Arvind Raman
    Soft Matter 16 64 - 81 2020年 [査読有り][通常論文]
  • Stiffness of brewers’ yeast under ethanol stress investigated by atomic force microscopy
    K. Toyota, R. Tanaka, T. Okajima
    Japanese Journal of Applied Physics 59 SN1005  2020年 [査読有り][通常論文]
  • Relationship between rheological properties and actin filaments of single cells investigated by atomic force microscopy
    R. Tanaka, M. Sawano, Y. Fujii, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, T. Okajima*
    Japanese Journal of Applied Physics 59 SN1010  2020年 [査読有り][通常論文]
  • Yuki Fujii, Yuki Ochi, Masahiro Tuchiya, Mihoko Kajita, Yasuyuki Fujita, Yukitaka Ishimoto, Takaharu Okajima
    Biophysical journal 116 6 1152 - 1158 2019年03月19日 [査読有り][通常論文]
     
    For isolated single cells on a substrate, the intracellular stiffness, which is often measured as the Young's modulus, E, by atomic force microscopy (AFM), depends on the substrate rigidity. However, little is known about how the E of cells is influenced by the surrounding cells in a cell population system in which cells physically and tightly contact adjacent cells. In this study, we investigated the spatial heterogeneities of E in a jammed epithelial monolayer in which cell migration was highly inhibited, allowing us to precisely measure the spatial distribution of E in large-scale regions by AFM. The AFM measurements showed that E can be characterized using two spatial correlation lengths: the shorter correlation length, lS, is within the single cell size, whereas the longer correlation length, lL, is longer than the distance between adjacent cells and corresponds to the intercellular correlation of E. We found that lL decreased significantly when the actin filaments were disrupted or calcium ions were chelated using chemical treatments, and the decreased lL recovered to the value in the control condition after the treatments were washed out. Moreover, we found that lL decreased significantly when E-cadherin was knocked down. These results indicate that the observed long-range correlation of E is not fixed within the jammed state but inherently arises from the formation of a large-scale actin filament structure via E-cadherin-dependent cell-cell junctions.
  • Cricket tympanal organ revisited: morphology, development, and possible functions of the adult-specific chitin core beneath the anterior tympanal membrane
    H. Nishino, M. Domae, T. Takanashi, T. Okajima
    Cell and Tissue Research 1 - 22 2019年 [査読有り][通常論文]
  • Calibrating the Young’s modulus of soft materials with surface tilt angle measured by atomic force microscopy
    Y. Fujii, T. Okajima
    AIP Advances 9 015028  2019年 [査読有り][通常論文]
  • Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin
    M. Tanaka, Y. Fujii, K. Hirano, T. Higaki, A. Nagasaki, R. Ishikawa, T. Okajima, K. Katoh
    Genes to Cells 24 202 - 213 2019年 [査読有り][通常論文]
  • Visualising the dynamics of live pancreatic microtumours self-organised through cell-in-cell invasion
    Y. Miyatake, K. Kuribayashi-Shigetomi, Y. Ohta, S. Ikeshita, A. Subagyo, K. Sueoka, A. Kakugo, M. Amano, T. Takahashi, T. Okajima, M. Kasahara
    Scientific Reports 8 14054  2018年 [査読有り][通常論文]
  • Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructure
    Q. He, T. Okajima, H. Onoe, A. Subagyo, K. Sueoka, K. Kuribayashi-Shigetomi
    Scientific Reports 8 4556  2018年 [査読有り][通常論文]
  • Regulation of Neuritogenesis in Hippocampal Neurons using Stiffness of Extracellular Microenvironment
    A. Tanaka, Y. Fujii, N. Kasai, T. Okajima, H. Nakashima
    PLOS ONE 13 e019192  2018年 [査読有り][通常論文]
  • PingGen Cai, Ryosuke Takahashi, Kaori Kuribayashi-Shigetomi, Agus Subagyo, Kazuhisa Sueoka, John M. Maloney, Krystyn J. Van Vliet, Takaharu Okajima
    Biophysical Journal 113 3 671 - 678 2017年08月 [査読有り][通常論文]
     
    Changes in the cytoskeletal organization within cells can be characterized by large spatial and temporal variations in rheological properties of the cell (e.g., the complex shear modulus G(star)). Although the ensemble variation in G(star) of single cells has been elucidated, the detailed temporal variation of G(star) remains unknown. In this study, we investigated how the rheological properties of individual fibroblast cells change under a spatially confined environment in which the cell translational motion is highly restricted and the whole cell shape remains unchanged. The temporal evolution of single-cell rheology was probed at the same measurement location within the cell, using atomic force microscopy-based oscillatory deformation. The measurements reveal that the temporal variation in the power-law rheology of cells is quantitatively consistent with the ensemble variation, indicating that the cell system satisfies an ergodic hypothesis in which the temporal statistics are identical to the ensemble statistics. The autocorrelation of G(star) implies that the cell mechanical state evolves in the ensemble of possible states with a characteristic timescale.
  • Mechanical properties of paraformaldehyde‑treated individual cells investigated by atomic force microscopy and scanning ion conductance microscopy
    S.‐O. Kim, J. Kim, T. Okajima, N.‐J. Cho
    Nano Convergence 4 5  2017年 [査読有り][通常論文]
  • Ryosuke Takahashi, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS 55 8 08NB22  2016年08月 [査読有り][通常論文]
     
    We investigated how stress relaxation mapping is quantified compared with the force modulation mapping of confluent epithelial cells using atomic force microscopy (AFM). Using a multi-frequency AFM technique, we estimated the power-law rheological behaviors of cells simultaneously in time and frequency domains. When the power-law exponent alpha was low (< 0.1), the alpha values were almost the same in time and frequency domains. On the other hand, we found that at the high values (alpha > 0.1), alpha in the time domain was underestimated relative to that in the frequency domain, and the difference increased with alpha, whereas the cell modulus was overestimated in the time domain. These results indicate that power-law rheological parameters estimated by stress relaxation are sensitive to lag time during initial indentation, which is inevitable in time-domain AFM experiments. (C) 2016 The Japan Society of Applied Physics.
  • Ryosuke Takahashi, Takaharu Okajima
    APPLIED PHYSICS LETTERS 107 17 2015年10月 [査読有り][通常論文]
     
    We present multi-frequency force modulation atomic force microscopy (AFM) for mapping the complex shear modulus G* of living cells as a function of frequency over the range of 50-500 Hz in the same measurement time as the single-frequency force modulation measurement. The AFM technique enables us to reconstruct image maps of rheological parameters, which exhibit a frequency-dependent power-law behavior with respect to G*. These quantitative rheological measurements reveal a large spatial variation in G* in this frequency range for single cells. Moreover, we find that the reconstructed images of the power-law rheological parameters are much different from those obtained in force-curve or single-frequency force modulation measurements. This indicates that the former provide information about intracellular mechanical structures of the cells that are usually not resolved with the conventional force measurement methods. (C) 2015 AIP Publishing LLC.
  • Aya Tanaka, Ryosuke Tanaka, Nahoko Kasai, Shingo Tsukada, Takaharu Okajima, Koji Sumitomo
    JOURNAL OF STRUCTURAL BIOLOGY 191 1 32 - 38 2015年07月 [査読有り][通常論文]
     
    Apoptosis plays an important role in many physiologic and pathologic conditions. The biochemical and morphological characteristics of apoptosis including cellular volume decrease, cell membrane blebbing, and phosphatidylserine translocation from the inner to the outer leaflet of the cell membrane are considered important events for phagocyte detection. Despite its importance, the relationship between the biological and morphological changes in a living cell has remained controversial. Scanning ion conductance microscopy is a suitable technique for investigating a series of these changes, because it allows us to observe the morphology of living cells without any mechanical interactions between the probe and the sample surface with a high resolution. Here, we investigated the biochemical and morphological changes in single neurons during the early stages of apoptosis, including apoptotic volume decrease, membrane blebbing and phosphatidylserine translocation, by using scanning ion conductance microscopy. Time-course imaging of apoptotic neurons showed there was a reduction in apoptotic volume after exposure to staurosporine and subsequent membrane bleb formation, which has a similar onset time to phosphatidylserine translocation. Our results show that a reduction in cellular volume is one of the earliest morphological changes in apoptosis, and membrane blebbing and phosphatidylserine translocation occur as subsequent biological and morphological changes. This is the first report to describe this series of morphological and biochemical changes ranging from an apoptotic volume decrease to membrane blebbing and PS translocation by scanning ion conductance microscopy (SICM). This new and direct imaging technique will provide new insight into the relationship between biochemical events inside a cell and cellular morphological changes. (C) 2015 Elsevier Inc. All rights reserved.
  • PingGen Cai, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS 54 3 2015年03月 [査読有り][通常論文]
     
    The mechanical properties of compliant single cells are significantly associated with various cell functions. It is thus crucially important in the identification and sorting of cells to characterize not only the complex moduli that exhibit power-law rheology but also the cell-to-cell variation at the single cell level. Atomic force microscopy (AFM) can be used to measure cellular mechanical properties and to quantify cell-to-cell variation. However, less is known about how precisely and routinely the cell-to-cell variation is obtained when the complex moduli vary substantially in different cell samples. Here, we investigate the storage modulus G' for single cells measured at controlled positions by AFM. We find that the spatial dependence of the frequency-dependent component of the cell-to-cell variation is preserved even if the spatial heterogeneity of G' is changed with the cell sample. The invariance of the frequency-dependent cell-to-cell variation indicates the robustness of AFM for the mechanical diagnosis of single cells. (C) 2015 The Japan Society of Applied Physics
  • Mihoko Kajita, Kaoru Sugimura, Atsuko Ohoka, Jemima Burden, Hitomi Suganuma, Masaya Ikegawa, Takashi Shimada, Tetsuya Kitamura, Masanobu Shindoh, Susumu Ishikawa, Sayaka Yamamoto, Sayaka Saitoh, Yuta Yako, Ryosuke Takahashi, Takaharu Okajima, Junichi Kikuta, Yumiko Maijima, Masaru Ishii, Masazumi Tada, Yasuyuki Fujita
    NATURE COMMUNICATIONS 5 2014年07月 [査読有り][通常論文]
     
    Recent studies have shown that certain types of transformed cells are extruded from an epithelial monolayer. However, it is not known whether and how neighbouring normal cells play an active role in this process. In this study, we demonstrate that filamin A and vimentin accumulate in normal cells specifically at the interface with Src- or RasV12-transformed cells. Knockdown of filamin A or vimentin in normal cells profoundly suppresses apical extrusion of the neighbouring transformed cells. In addition, we show in zebrafish embryos that filamin plays a positive role in the elimination of the transformed cells. Furthermore, the Rho/Rho kinase pathway regulates filamin accumulation and filamin acts upstream of vimentin in the apical extrusion. This is the first report demonstrating that normal epithelial cells recognize and actively eliminate neighbouring transformed cells and that filamin is a key mediator in the interaction between normal and transformed epithelial cells.
  • Ryosuke Takahashi, Satoshi Ichikawa, Agus Subagyo, Kazuhisa Sueoka, Takaharu Okajima
    ADVANCED ROBOTICS 28 7 449 - 455 2014年04月 [査読有り][通常論文]
     
    To investigate the mechanical properties of adherent cells under conditions where cell-to-cell interaction can be prevented and controlled, we used microcontact printing to pattern single cells without intercellular contacts and measured them with a custom-built atomic force microscopy (AFM) system. The motorized AFM system can measure individual cells over a large spatial range, enabling the measurement of cells in a microarray format. We tested single fibroblast cells and found that the power-law of their complex shear modulus is not significantly influenced by cell-to-cell contact. This method is effective for obtaining high-throughput measurements on single cells.
  • Pinggen Cai, Yusuke Mizutani, Masahiro Tsuchiya, John M. Maloney, Ben Fabry, Krystyn J. Van Vliet, Takaharu Okajima
    Biophysical Journal 105 5 1093 - 1102 2013年09月03日 [査読有り][通常論文]
     
    Among individual cells of the same source and type, the complex shear modulus exhibits a large log-normal distribution that is the result of spatial, temporal, and intrinsic variations. Such large distributions complicate the statistical evaluation of pharmacological treatments and the comparison of different cell states. However, little is known about the characteristic features of cell-to-cell variation. In this study, we investigated how this variation depends on the spatial location within the cell and on the actin filament cytoskeleton, the organization of which strongly influences cell mechanics. By mechanically probing fibroblasts arranged on a microarray, via atomic force microscopy, we observed that the standard deviation σ of was significantly reduced among cells in which actin filaments were depolymerized. The parameter σ also exhibited a subcellular spatial dependence. Based on our findings regarding the frequency dependence of σ of the storage modulus G′, we proposed two types of cell-to-cell variation in G′ that arise from the purely elastic and the frequency-dependent components in terms of the soft glassy rheology model of cell deformability. We concluded that the latter inherent cell-to-cell variation can be reduced greatly by disrupting actin networks, by probing at locations within the cell nucleus boundaries distant from the cell center, and by measuring at high loading frequencies. © 2013 Biophysical Society.
  • Yusuke Mizutani, Myung-Hoon Choi, Sang-Joon Cho, Takaharu Okajima
    APPLIED PHYSICS LETTERS 102 17 2013年04月 [査読有り][通常論文]
     
    Nanoscale fluctuations on the apical surfaces of epithelial cells connected to neighboring cells were investigated by scanning ion conductance microscopy. Mapping the ion current as a function of the tip-surface distance revealed that in untreated cells, the apparent fluctuation amplitude increased towards the cell center. We found that the spatial dependence was less correlated with the heterogeneities of cell stiffness but was significantly reduced when actin filaments were disrupted. The results indicate that apical surface fluctuations are highly constrained at the cell-cell interface, in the vertical direction to the surface and by the underlying actin filaments. (C) 2013 AIP Publishing LLC.
  • Yusuke Mizutani, Zen Ishikura, Myung-Hoon Choi, Sang-Joon Cho, Takaharu Okajima
    BIOPHYSICAL JOURNAL 104 2 317A - 317A 2013年01月 [査読有り][通常論文]
  • Yusuke Mizutani, Koichi Kawahara, Takaharu Okajima
    Current Pharmaceutical Biotechnology 13 14 2599 - 2603 2012年 [査読有り][通常論文]
     
    Inotropic agents induce changes in the contraction amplitude and frequency of cardiomyocytes (CMs). However, it is unknown how local contractions of CMs treated by inotropic agents behave spatiotemporally. In this study, the effect of isoproterenol, a positive inotropic agent, on local contractions of isolated neonatal rat CMs was explored by atomic force microscopy (AFM). We observed that changes in local contraction amplitude of CM in the presence of isoproterenol were heterogeneous they were unchanged or increased, at different positions, with respect to the amplitude of untreated CMs. Interestingly, spatial heterogeneities of local contraction amplitude of CM in the presence of isoproterenol did not obviously correlate with the local elasticity, indicating that the local contractions were facilitated by cooperative dynamics of the cytoskeletal structure in relatively large regions, rather than those just under AFM indentation. Moreover, local contraction amplitude of CM in the presence of isoproterenol was not proportional to that in the control condition, showing that the former change was no longer additive in local scales. © 2012 Bentham Science Publishers.
  • Takaharu Okajima
    Current Pharmaceutical Biotechnology 13 14 2623 - 2631 2012年 [査読有り][通常論文]
     
    Rheological properties of living cells play important roles in regulating their various biological functions. Therefore, measuring cell rheology is crucial for not only elucidating the relationship between the cell mechanics and functions, but also mechanical diagnosis of single cells. Atomic force microscopy (AFM) is becoming a useful technique for single cell diagnosis because it allows us to measure the rheological properties of adherent cells at any region on the surface without any modifications. In this review, we summarize AFM techniques for examining single cell rheology in frequency and time domains. Recent applications of AFM for investigating the statistical analysis of single cell rheology in comparison to other micro-rheological techniques are reviewed, and we discuss what specificity and universality of cell rheology are extracted using AFM. © 2012 Bentham Science Publishers.
  • Akinori Okada, Yusuke Mizutani, Agus Subagyo, Hirotaka Hosoi, Motonori Nakamura, Kazuhisa Sueoka, Koichi Kawahara, Takaharu Okajima
    APPLIED PHYSICS LETTERS 99 26 263703  2011年12月 [査読有り][通常論文]
     
    We investigated dynamic force propagation between focal adhesions of fibroblast cells cultured on polydimethylsiloxane micropost substrates, by atomic force microscopy. Live cells were mechanically modulated by the atomic force microscopy probe bound to cell apical surfaces at 0.01-0.5 Hz, while microposts served as a force sensor at basal surfaces. We observed that cells exhibited rheological behavior at the apical surface but had no apparent out-of-phase response at the basal surface, indicating that the dynamic force propagating through cytoskeletal filaments behaves in an elastic manner. Moreover, the direction of the propagated force was observed to be intimately associated with the prestress. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3672225]
  • Kunugi, Satohiko, Iwabuchi, Sadahiro, Matsuyama, Daisuke, Okajima, Takaharu, Kawahara, Koichi
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 416 3-4 409 - 415 2011年12月 [査読有り][通常論文]
     
    Extracellular ATP acts as a potent agonist on cardiomyocytes, inducing a broad range of physiological responses via P2 purinoceptors. Its concentration in the interstitial space within the heart is elevated during ischemia or hypoxia due to its release from a number of cell types, including cardiomyocytes. However, the exact mechanism responsible for the release of ATP from cardiomyocytes during ischemia is not known. In this study, we investigated whether and how the release of ATP was strictly regulated during ischemia in cultured neonatal rat cardiomyocytes. lschemia was mimicked by oxygen-glucose deprivation (OGD). Exposure of cardiomyocytes to OGD resulted in an increase in the concentration of extracellular ATP shortly after the onset of OGD (15 min), and the increase was reversed by treatment with blockers of maxi-anion channels. Unexpectedly, at 1 and 2 h after the onset of OGD, the blocking of maxi-anion channels increased the concentration of extracellular ATP, and the increase was significantly suppressed by co-treatment with blockers of hemichannels, suggesting that ATP release via maxi-anion channels was involved in the suppression of ATP release via hemichannels during persistent OGD. Here we show the possibility that the release of ATP from cardiomyocytes was strictly regulated during ischemia by negative-feedback mechanisms; that is, maxi-anion channel-derived ATP-induced suppression of ATP release via hemichannels in cardiomyocytes. (C) 2011 Elsevier Inc. All rights reserved.
  • Atsushi Miyaoka, Yusuke Mizutani, Masahiro Tsuchiya, Koichi Kawahara, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS 50 8 08LB16  2011年08月 [査読有り][通常論文]
     
    The rheological properties of growth-arrested and quiescent (G0 phase) mouse fibroblast cells under serum starvation were investigated by atomic force microscopy (AFM) with a microarray technique. The number distribution of complex shear modulus, G*, of quiescent cells at the serum concentration, C-S = 0.1%, followed a log-normal distribution, and the frequency dependence of G* exhibited a power law behavior, which were similar to those under a control condition at CS 10%. On the other hand, we found that the Newtonian viscosity coefficient of the quiescent cells significantly increased, and the distribution broadened, as compared with the control cells, whereas the power-law exponent was unchanged. The result indicated that the rheological properties of quiescent fibroblast cells were not identical to those in the G1 phase during cell cycle. This finding suggests that the Newtonian viscosity of cells is one of the useful indicators for evaluating growth-arrested cells under serum starvation. (C) 2011 The Japan Society of Applied Physics
  • Kenichi Niikura, Katsuyuki Nambara, Takaharu Okajima, Ryosuke Kamitani, Shin Aoki, Yasutaka Matsuo, Kuniharu Ijiro
    ORGANIC & BIOMOLECULAR CHEMISTRY 9 16 5787 - 5792 2011年 [査読有り][通常論文]
     
    In our previous paper, secondary-amine appended cationic polymer 1 was used as a scaffold to display artificial receptors on a cell surface (R. Kamitani et al., ChemBioChem, 2009, 10, 230). This polymer can be retained on the cell surface for more than 30 min before being slowly internalized into the cells. In this study, our aim is to achieve the efficient internalization of quantum dots (QDs) into target cells via artificial receptors on the polymer. As a receptor molecule, N-acetylglucosamine (GlcNAc) moieties were introduced into the polymer, and GlcNAc binding protein-displaying QDs were used as a ligand. We found that ligand-presenting QDs could be internalized effectively into cells via polymer-mediated endocytosis, whereas QDs were not internalized into untreated cells. These data suggest that our method based on cell-surface engineering using polymers affords a new approach to the delivery of various poorly permeable nanoparticles into cells.
  • Kenichi Niikura, Katsuyuki Nambara, Takaharu Okajima, Yasutaka Matsuo, Kuniharu Ijiro
    LANGMUIR 26 12 9170 - 9175 2010年06月 [査読有り][通常論文]
     
    A series of FITC-labeled hydrophobic molecules (1-8) were prepared, and their cellular uptakes have been investigated using cell-cycle-synchronized HeLa cells. The cellular membrane permeability of compounds strongly depended on both the chemical structure and the cell-cycle phase. In the G1/S phase, branched hydrocarbon-containing 3 and cis-olefin-containing 2 and 8 were efficiently internalized into cells by passive diffusion. In contrast, linear alkyl chain-containing 1 and 7 were retained on the membrane without rapid internalization. In the M phase, rapid permeation was suppressed for all molecules.
  • Statistics of Single Cell Mechanics Investigated by Atomic Force Microscopy
    Hiratsuka, Y. Mizutani, P.G. Cai, M. Tsuchiya, H. Tokumoto, K. Kawahara, T. Okajima
    MRS Spring Meeting Symposium U proceedings 9999  2010年 [査読有り][通常論文]
  • Shinichiro Hiratsuka, Yusuke Mizutani, Akitoshi Toda, Norichika Fukushima, Koichi Kawahara, Hiroshi Tokumoto, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS 48 8 08JB14-4  2009年08月 [査読有り][通常論文]
     
    We measured stress and creep relaxations of mouse fibroblast cells arranged and cultured on a microarray, by colloidal probe atomic force microscopy (AFM). A hydrophobic monolayer coating of perfluorodecyltrichlorosilane (FDTS) on the surface of colloidal silica beads significantly reduced the adhesion force of live cells, compared with untreated beads. The rheological behaviors of cells were estimated by averaging several relaxation curves of cells measured by the AFM. Longer-time tailing of both stress and creep relaxation curves followed single power-law behavior over a time scale of 60s, with exponents in the range 0.1-0.4, varying with cells. The results were in good agreement with previous measurements of the frequency-domain rheology of cells using the force modulation mode. (C) 2009 The Japan Society of Applied Physics
  • Shinichiro Hiratsuka, Yusuke Mizutani, Masahiro Tsuchiya, Koichi Kawahara, Hiroshi Tokumoto, Takaharu Okajima
    ULTRAMICROSCOPY 109 8 937 - 941 2009年07月 [査読有り][通常論文]
     
    The viscoelastic properties of a large number of mouse fibroblast NIH3T3 cells (n similar or equal to 130) were investigated by combining atomic force microscopy (AFM) with a microarray technique. In the experiments, the cells were arranged and cultured in the wells of a microarray substrate, and a force modulation mode experiment was used to measure the complex shear modulus, G*, of individual cells in a frequency range 0.5-200 Hz. The frequency dependence of G* of the cells exhibited a power-law behavior and similar frequency dependencies have been observed in several cell types cultured on flat substrates. This indicated that the NIH3T3 cells cultured in the wells of a microarray have analogous structural organization to those cells cultured on flat substrates. The number distribution of both the storage and loss moduli of G* fitted well to a log-normal distribution function, whereas the power-law exponent estimated by a power-law structural damping model showed a normal distribution function. These results showed that combining AFM with a microarray technique was a suitable approach for investigating the statistics of rheological properties of living cells without the requirement of cell surface modification. (c) 2009 Elsevier B.V. All rights reserved.
  • Ryosuke Kamitani, Kenichi Niikura, Takaharu Okajima, Yasutaka Matsuo, Kuniharu Ijiro
    CHEMBIOCHEM 10 2 230 - 233 2009年01月 [査読有り][通常論文]
  • Yoshimi Tanaka, Yasunori Kawauchi, Takayuki Kurokawa, Hidemitsu Furukawa, Takaharu Okajima, Jian Ping Gong
    MACROMOLECULAR RAPID COMMUNICATIONS 29 18 1514 - 1520 2008年09月 [査読有り][通常論文]
     
    Double-network (DN) gels, a type of interpenetrating polymer network (IPN) consisting of rigid and flexible polymer components, exhibit two outstanding mechanical behaviors: yielding deformation of the entire specimen in tensile tests and quite high fracture energy in tearing tests. In this study, atomic force microscope (AFM) measurements were conducted on DN gels to determine the local Young's moduli immediately below the fracture surfaces E-f and below the usual molded surfaces E-m, and compare the local modulus with bulk Young's moduli measured before and after the yielding deformation, denoted as E-h and E-s, respectively. E-m and E-h are around 0.1 MPa; E-f and E-s, around 0.01 MPa, one order lower than the former two moduli. The order relation indicates that yielding deformation occurred locally around the crack tip of the DN gel during fracture. This supports the basic assumption of phenomenological models recently proposed to explain high fracture energy of DN gels.
  • Yusuke Mizutani, Masahiro Tsuchiya, Shinichiro Hiratsuka, Koichi Kawahara, Hiroshi Tokumot, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS 47 7 6177 - 6180 2008年07月 [査読有り][通常論文]
     
    The number distribution of the elastic modulus of fibroblast Cells Was successfully measured during the early stages of adhesion using an atomic force microscope (AFM) combined with a microarray as a substrate, which allowed us to arrange and Culture cells so that a large number of cells could be measured in a short time period. We confirmed that the cells deposited in the wells of the microarray could be cultured for at least 12 h without any significant migration. Histograms of the Young's modulus. E. of the cells during the early stages of adhesion produced from force curve measurements of cells (n congruent to 300) cultured for 3-9 h were well fitted to a log-normal distribution function. With increasing incubation time, the average value of E increased significantly, while the standard deviation of the distribution remained almost constant. The results are discussed in terms of the cytoskeleton inside cells.
  • Hiroshi Yamamura, Katsuki Kimura, Takaharu Okajima, Hiroshi Tokumoto, Yoshimasa Watanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 42 14 5310 - 5315 2008年07月 [査読有り][通常論文]
     
    Fouling in membranes used for water treatment has been attributed to the presence of natural organic matter (NOM) in water. There have been reports recently on the contribution of hydrophilic fractions of NOM(e.g., carbohydrate-like substances) to fouling, but there is still little information about the physicochemical interactions between membranes and carbohydrate-like substances. In this study, the affinity of carbohydrate-like substances to two different microfiltration (MF) membranes was investigated by using atomic force microscopy (AFM) and functionally modified microspheres. Microspheres were attached to the tip of the cantilever in an AFM apparatus and the adhesion forces working between the microspheres and the membranes were determined. The microspheres used in this study were coated with either hydroxyl groups or carboxyl groups to be used as surrogates of carbohydrate-like substances or humic acid, respectively. Measurements of adhesion force were carried out at pH of 6.8 and the experimental results demonstrated that the adhesion force to membranes was strong in the case of hydroxyl groups but weak in the case of carboxyl groups. The strong adhesion between the hydroxyl group and the membrane surface is explained by the strong hydrogen bond generated. It was also found that the affinity of the hydroxyl group to a polyvinylidenefluoride (PVDF) membrane was much higher than that to a polyethylene (PE) membrane, possibly due to the high electronegative nature of the PVDF polymer. The time course of changes in the affinity of hydroxyl group to a membrane used in a practical condition was investigated by repeatedly carrying out AFM force measurements with PE membrane specimens sampled from a pilot plant operated at an existing water treatment plant. Microspheres exhibited strong affinity to the membrane at the initial stage of operation (within 5 days), but subsequently exponential reduction of the affinity was seen until the end of operation, as a result of fouling development. However, the magnitude of affinity of hydroxyl-modified microspheres was much higher than that of carboxyl-modified microspheres even after the significant reduction of affinity of hydroxyl-modified microspheres to the membranes was seen. The results obtained in this study partially explain why hydrophilic NOM dominated over humic substances in foulants of membranes used for water treatment in recent studies on fouling.
  • Kazuhiro Shikinaka, Hyuckjoon Kwon, Akira Kakugo, Hidemitsu Furukawa, Yoshihito Sada, Jian Ping Gong, Yoshitaka Aoyama, Hideo Nishioka, Hiroshi Jinnai, Takaharu Okajima
    BIOMACROMOLECULES 9 2 537 - 542 2008年02月 [査読有り][通常論文]
     
    Three-dimensional structures of actin bundles formed with polycations were observed by using transmission electron microtomography and atomic force microscopy. We found, for the first time, that the cross-sectional morphology of actin bundles depends on the polycation species and ionic strength, while it is insensitive to the degree of polymerization and concentration of polycation. Actin bundles formed with poly-N-[3-(dimethylamino)propyl] acrylamide methyl chloride quaternary show a ribbon-like cross-sectional morphology in low salt concentrations that changes to cylindrical cross-sectional morphology with hexagonal packing of the actin filaments in high salt concentrations. Contrastingly, actin bundles formed with poly-L-lysine show triangular cross-sectional morphology with hexagonal packing of the actin filaments. These variations in cross-sectional morphology are discussed in terms of anisotropy in the electrostatic energy barrier.
  • Takaharu Okajima, Masaru Tanaka, Shusaku Tsukiyama, Tsubasa Kadowaki, Sadaaki Yamamoto, Masatsugu Shimomura, Hiroshi Tokumot
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS 46 8B 5552 - 5555 2007年08月 [査読有り][通常論文]
     
    We measured the stress relaxation of mouse fibroblast NIH3T3 cells with an atomic force microscope (AFM) using a sharp silicon tip and a silica bead with a radius of similar to 1 mu m as an indenter. The decay of loading force was clearly observed in NIH3T3 cells at a small initial loading force of similar to 0.4 nN and was well fitted to the stretched exponential function rather than to a single exponential function. The stretching exponent parameter was similar to 0.5 for both indenters, indicating that the stress relaxation observed in NIH3T3 cells consisted of multiple relaxation processes. The time-domain AFM technique described in this report allows us to measure directly the relaxation process of living cells in a range from milliseconds to seconds.
  • Hyuck Joon Kwon, Akira Kakugo, Takehiro Ura, Takaharu Okajima, Yoshimi Tanaka, Hidemitsu Furukawa, Yoshihito Osada, Jian Ping Gong
    LANGMUIR 23 11 6257 - 6262 2007年05月 [査読有り][通常論文]
     
    We show that F-actins form three-dimensional giant network under uni-directional diffusion of polycations, at a dilute actin concentration (0.01 mg/mL) that only bundles are formed by homogeneous mixing with polycations. The mesh size of the actin network depends on polycation concentration and ionic strength, while bundle thickness of network depends only on ionic strength, which indicates that actin network is formed through nucleation-growth mechanism. The mesh size and the bundle thickness are determined by nucleus concentration and nucleus size, respectively. The atomic force microscopy measurement correlates the elasticity of the actin network, E, with the mesh size, xi, as E similar to xi(-1), while the bundle thickness, D dependence of E cannot be described by a simple scaling relation. E similar to D-6.5 when D is small and E similar to D-0.1 when D is large. Our study on the self-assembly of actin network under asymmetric polycation condition would provide the crucial insight into the organization of biopolymers in polarized condition of cell.
  • Takaharu Okajima, Xiang-Ming Tao, Hiroaki Azehara, Hiroshi Tokumoto
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 7 3 790 - 795 2007年03月 [査読有り][通常論文]
     
    We conducted the extraction experiments of single polymer incorporated into hydrogels with an atomic force microscope (AFM) as a model for investigating nonspecific intermolecular interactions between macromolecules in a semidilute region at the single molecule level. Small amount of poly(ethylene glycol) (PEG) terminated with a thiol group was inserted in poly(acrylamide) gels, and a part of PEG polymer segments on the gel surface was attempted to pull out of the gels with a gold-coated AFM tip. The observed force-distance curves were classified into two kinds of extraction force profiles: a plateau force, which is almost constant irrespective of the tip-surface distance and a nonlinear force, which nonlinearly increases with the extraction length. Characteristic interaction length, L, and force, F, of these extraction force profiles were measured with changing the crosslinker concentration of gels which strongly affects the network structures. As a result, L of these extraction profiles significantly decreased at crosslinker concentrations higher than a standard one at which most gels have been prepared for investigating their physical properties. On the other hand, F showed no obvious difference on the change in crosslinker concentrations both on the plateau and the nonlinear force profiles. The origin of the observed forces was discussed in terms of gel network structures.
  • Hiroaki Kii, Takuya Takagi, Akira Sasaki, Takaharu Okajima, Masataka Kinjo
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 7 3 726 - 729 2007年03月 [査読有り][通常論文]
     
    Liverwort-like DNA microscale structures consist of 4-sticky-end Holiday junctions as DNA bricks that can be used in nanotechnology and nanobiotechnology to direct the self-assembly of nanomachines as well as DNA assembly. Previously it has not been possible to obtain such DNA microscale structural forms, but herein we report construction of a mesh-like material made up of 4 strands of 40-base DNA. Advanced bioimaging techniques such as fluorescence correlation spectroscopy (FCS), laser scanning microscopy (LSM), and atomic force microscopy (AFM) help us as ultrasensitive detection tools for examing structures in solutions. Combinations of these techniques allow us to survey various chemical conditions of materials and solutions.
  • T. Okajima, M. Tanaka, S. Tsukiyama, T. Kadowaki, S. Yamamoto, M. Shimomura, H. Tokumoto
    NANOTECHNOLOGY 18 8 084010  2007年02月 [査読有り][通常論文]
     
    Stress relaxation of HepG2 cells was examined with an atomic force microscope ( AFM). In the measurement, a loading force was applied to the cell by an AFM tip, and a time series of the cantilever deflection signal was measured at a fixed position of the cantilever base displacement. The relaxation of the loading force was clearly observed on the HepG2 cells, and was well fitted to a stretched exponential function known as the Kohlrausch-Williams-Watts ( KWW) function, which is empirically employed to represent dispersion processes of the system. The relaxation time and the stretching exponent parameter were estimated to be similar to 0.5 s and 0.4 - 0.6, respectively. The latter indicated that the relaxation observed in HepG2 cells consisted of multiple relaxation processes. Moreover, it was found that the characteristic feature of the relaxation process was not strongly correlated with the elastic properties of the cells.

書籍

  • 重川, 秀実, 吉村, 雅満, 目良, 裕, 岡嶋, 孝治 
    近代科学社 2020年08月 (ISBN: 9784764950290) x, 179p, 図版 [4]p
  • 原子間力顕微鏡法:細胞表面近傍のナノメカニクス
    藤井裕紀, 岡嶋孝治 
    表面と真空、63、437-440 (2020) 2020年
  • AFMによる多細胞系の力学測定
    藤井裕紀, 岡嶋孝治 (担当:分担執筆)
    高分子、68、656-657 2019年12月
  • AFMを用いた1細胞・細胞集団の力学物性計測
    藤井裕紀, 岡嶋孝治 (担当:分担執筆)
    生体の科学(特集:メカノバイオロジー)vol.70, No.4 2019年07月
  • 細胞ナノレオロジー:原子間力顕微鏡法
    岡嶋 孝治 (担当:分担執筆)
    月刊ソフトマター 2018年09月
  • 走査型プローブ顕微鏡
    淺川雅, 岡嶋孝治, 大西洋 (担当:共著)
    日本分析化学会編、分析化学実技シリーズ機器分析編15 2017年
  • 原子間力顕微鏡による1細胞レオロジーの定量計測─細胞の個性とエルゴード性
    岡嶋 孝治 (担当:単著)
    応用物理学会誌、86、1052-1056 2017年
  • Nanorheology of Living Cells
    T. Okajima (担当:分担執筆範囲:The World of Nano-Biomechanics (Chapter 13, A. Ikai))
    Elsevier 2016年
  • 原子間力顕微鏡を用いた細胞の力学特性計測
    岡嶋孝治, 高橋亮輔 (担当:分担執筆範囲:細胞の特性計測・操作と応用(組織工学ライブラリ-マイクロロボティクスとバイオの融合- 1)(第1章・第3節))
    コロナ社 2016年
  • Atomic Force Microscopy: Imaging and Rheology of Living Cells
    Takaharu Okajima (担当:共著)
    Nano/Micro Science and Technology in Biorheology: Principles, Methods, and Applications Chapter 15 (387-414), Springer, (2015) 2015年
  • 原子間力顕微鏡を用いた細胞レオロジー特性の計測
    岡嶋 孝治 (担当:共著)
    三次元ティッシュエンジニアリング~細胞の培養・操作・組織化から品質管理、脱細胞化まで~(第1章・第3節)、(株式会社NTS) (2015) 2015年
  • イオンコンダクタンス顕微鏡:細胞膜表面の揺らぎを計測する
    水谷祐輔, 田中良昌, 田中あや, 住友弘二, 岡嶋孝治 (担当:共著)
    光学-細胞機能に迫る非染色非破壊イメージング技術、44, (6月号)(2015) 2015年
  • High-throughput measurements of single cell rheology by atomic force microscopy
    K. Kuribayashi-Shigetomi, R. Takahashi, A. Subagyo, K. Sueoka, T. Okajima (担当:共著)
    Hyper Bio Assembler for 3D Cellular Systems, Chapter 4 (57-67), Springer, 2015 2015年
  • 原子間力顕微鏡による超高速細胞メカニクス計測技術
    高橋亮輔, 岡嶋孝治 
    ケミカルエンジニヤリング(先端計測技術開発の展望:9月号)(2015) 2015年
  • 細胞の物理学:SPMによる定量計測
    岡嶋 孝治 (担当:共著)
    表面科学 35, 544-544(2014) 2014年
  • 走査プローブ顕微鏡による細胞物性測定:原子間力顕微鏡とイオンコンダクタンス顕微鏡(総説)
    岡嶋孝治 
    化学工業 (2013) 2013年
  • 原子間力顕微鏡による細胞力学の定量計測(解説)
    岡嶋 孝治 
    検査技術 (2013) 2013年
  • 原子間力顕微鏡による細胞レオロジー測定(総説)
    岡嶋孝治 
    日本膜学会「膜」38, 76-81 (2013) 2013年
  • 1細胞レオロジー測定 : 原子間力顕微鏡による細胞レオロジー計測の新手法
    岡嶋 孝治, 水谷 祐輔 
    生物物理 52, 5, 230-233 2012年09月
  • 細胞力学計測のためのマイクロ加工基板を用いた原子間力顕微鏡法
    岡嶋 孝治, 水谷 祐輔 
    表面科学33(8) 461-466 2012年08月
  • 1細胞レオロジー測定:原子間力顕微鏡による細胞レオロジー計測の新手法(総説)
    岡嶋孝治, 水谷祐輔 
    日本生物物理学会誌「生物物理」52, 230-233 (2012) 2012年
  • 走査プローブ顕微鏡(実験物理科学シリーズ6)
    共立出版 2009年
  • 原子間力顕微鏡による生細胞ナノレオロジー計測
    岡嶋孝治, 徳本洋志 (担当:分担執筆)
    日本レオロジー学会誌 vol.36, No.2, p. 81-86 2008年04月
  • ナノテクのための物理入門
    共立出版 2007年
  • BioNics[バイオニクス]
    オーム社 2005年
  • ソフトナノテクノロジー —バイオマテリアル革命—
    シーエムシー出版 2005年

その他活動・業績

  • Shunsuke Hirotsu, Ikuo Yamamoto, Atsushi Matsuo, Takaharu Okajima, Hidemitsu Furukawa, Tatsuyuki Yamamoto Journal of the Physical Society of Japan 64 (8) 2898 -2907 1995年 [査読無し][通常論文]
     
    Brillouin scattering spectra of poly-N-isopropylacrylamide (NIPA) gels swollen to equilibrium in water were measured as a function of temperature both in the swollen and the shrunken phases. This is the first Brillouin scattering experiment on a gel undergoing a volume phase transition. At the discontinuous volume phase transition occuring at 33.6°C, large changes were observed in both of the Brillouin shift and the spectral width. From the measured spectra and the temperature-dependent refractive index of the gel, the hypersonic velocity and the attenuation were determined as a function of temperature. The results are analyzed in terms of the nature of sound propagation in gels, structure of the shrunken phase, relaxation processes of the network and solvent, and a role played by water molecules at the phase transition.

特許

  • 特願2017-55414:原子間力顕微鏡による単一細胞の力学的診断方法と診断装置  2017年03月22日
    岡嶋孝治, 高橋亮輔
  • 特願特願2016-176540:酵母細胞の力学特性の測定方法、及び力学特性に基づいた酵母細胞のスクリーニング方法  2016年09月09日
    有友亮太, 潮井徹, 岡嶋孝治, 田中良昌
  • 特願2017-032733:細胞足場材料、細胞モデル、及び細胞足場材料の製造方法  2016年02月23日
    田中あや, 手島哲彦, 中島寛, 岡嶋孝治, 藤井裕紀
  • 特願2014-136721:細胞の複素弾性率の計測方法および計測システム  2014年07月02日
    岡嶋孝治, 高橋亮輔
  • 特願2011-184403:単一細胞の力学特性の計測方法および計測装置  2011年08月26日
    岡嶋孝治, 蔡萍根, 水谷祐輔, 土屋雅博  
    特願2011-184403
  • 原子間力顕微鏡用のコロイドプローブカンチレバーの製造方法および製造装置
    特願2007-287126
  • Surface Position Measuring Method and Surface Position Measuring Device
    WO 2006/073068
  • Device and Method for Measuring Molecule Using Gel Substrate Material
    WO 2006/028135
  • Molecular Measuring Device and Molecule Measuring Method
    WO 2006/011348

共同研究・競争的資金等の研究課題

  • マルチプローブ顕微鏡技術を用いた発生胚メカニクス計測法の開発
    挑戦的研究(萌芽)
    研究期間 : 2019年07月 -2021年03月 
    代表者 : 岡嶋 孝治
  • 細胞のエルゴード性を利用したがん細胞力学診断・アッセイ法の開発
    基盤研究(B)
    研究期間 : 2018年04月 -2021年03月 
    代表者 : 岡嶋 孝治
  • 原子間力顕微鏡を用いた発生胚メカニクスの網羅解析法の開発
    挑戦的研究(萌芽)
    研究期間 : 2017年04月 -2019年03月 
    代表者 : 岡嶋 孝治
  • ヒト間葉系幹細胞治療技術の開発とその最適化に関する研究
    二国間交流事業 日本—シンガポール 共同研究
    研究期間 : 2015年04月 -2017年03月 
    代表者 : 岡嶋 孝治
  • 多重周波数フォースモジュレーション顕微鏡の開発:細胞レオロジー変数のイメージング
    挑戦的萌芽研究
    研究期間 : 2015年04月 -2017年03月 
    代表者 : 岡嶋 孝治
  • 原子間力顕微鏡による生体組織力学物性のその場分離計測技術の開発
    新学術領域研究(研究領域提案型)バイオアセンブラ
    研究期間 : 2014年04月 -2016年03月 
    代表者 : 岡嶋 孝治
  • プローブ顕微鏡による細胞間力学的相互作用の時空間揺らぎの研究
    新学術領域研究(研究領域提案型) ゆらぎと構造
    研究期間 : 2014年04月 -2016年03月 
    代表者 : 岡嶋 孝治
  • 原子間力顕微鏡を用いた細胞力学伝播関数の時空間定量解析
    基盤研究(B)
    研究期間 : 2013年 -2015年 
    代表者 : 岡嶋 孝治
  • 原子間力顕微鏡による超高速細胞レオロジー分離技術の開発
    新学術領域研究(研究領域提案型)バイオアセンブラ
    研究期間 : 2012年04月 -2014年03月 
    代表者 : 岡嶋孝治
  • 原子間力顕微鏡を用いたがん細胞力学診断技術の開発
    挑戦的萌芽研究:
    研究期間 : 2011年04月 -2013年03月 
    代表者 : 岡嶋 孝治
  • 原子間力顕微鏡法を用いたがん細胞診断自動解析法の開発
    研究成果最適展開支援プログラム(A-STEP)
    研究期間 : 2012年 -2013年 
    代表者 : 岡嶋 孝治
  • 原子間力顕微鏡を用いた多数細胞力学の多変量解析の研究
    基盤研究(B):
    研究期間 : 2009年04月 -2012年03月 
    代表者 : 岡嶋 孝治
  • 細胞周期制御下の細胞界面ナノダイナミクスの研究
    特定領域研究・高次系分子科学:
    研究期間 : 2009年04月 -2011年03月 
    代表者 : 岡嶋 孝治
  • 走査プローブ顕微鏡と蛍光相関分光法による生細胞界面の1分子ダイナミクスの研究
    特定領域研究・高次系分子科学:
    研究期間 : 2008年04月 -2010年03月 
    代表者 : 岡嶋 孝治
  • 単一細胞表層の全方向ナノダイナミクス計測技術の開発
    NEDO:産業技術研究助成事業
    研究期間 : 2006年 -2009年 
    代表者 : 岡嶋孝治(代表)
  • 糖鎖1分子のナノ力学刺激に対する応答ダイナミクスの研究
    萌芽研究:
    研究期間 : 2005年04月 -2007年03月 
    代表者 : 岡嶋 孝治
  • 生体分子のマイクロ秒粘弾性測定
    若手研究(B):
    研究期間 : 2003年04月 -2005年03月 
    代表者 : 岡嶋 孝治
  • 原子間力顕微鏡を用いた単一高分子鎖の相転移における力学緩和の測定
    若手研究(B)
    研究期間 : 1999年04月 -2001年03月 
    代表者 : 岡嶋 孝治
  • シェアフォース顕微鏡におけるプローブ・表面間相互作用の起源の解明
    奨励研究(A)
    研究期間 : 1997年04月 -1999年03月 
    代表者 : 岡嶋 孝治
  • イオンコンダクタンス顕微鏡を用いた細胞膜揺らぎの定量イメージング技術の開発
    挑戦的萌芽研究
    代表者 : 岡嶋 孝治

教育活動情報

主要な担当授業

  • 細胞生物工学特論
    開講年度 : 2019年
    課程区分 : 修士課程
    開講学部 : 情報科学研究科
    キーワード : 細胞力学,非平衡非線形 cell mechanics, nonlinear and non-equilibrium phenomena
  • 細胞生物工学特論
    開講年度 : 2019年
    課程区分 : 修士課程
    開講学部 : 情報科学院
    キーワード : 細胞力学,非平衡非線形 cell mechanics, nonlinear and non-equilibrium phenomena
  • バイオエンジニアリング特論
    開講年度 : 2019年
    課程区分 : 修士課程
    開講学部 : 情報科学研究科
    キーワード : 遺伝情報, genetic information, バイオインフォマティクス, bioinformatics, イメージング, imaging, 生体医工学, biomedical engineering, 細胞力学, cell mechanics
  • 細胞生物工学特論
    開講年度 : 2019年
    課程区分 : 博士後期課程
    開講学部 : 情報科学研究科
    キーワード : 細胞力学,非平衡非線形 cell mechanics, nonlinear and non-equilibrium phenomena
  • 細胞生物工学特論
    開講年度 : 2019年
    課程区分 : 博士後期課程
    開講学部 : 情報科学院
    キーワード : 細胞力学,非平衡非線形 cell mechanics, nonlinear and non-equilibrium phenomena
  • バイオエンジニアリング特論
    開講年度 : 2019年
    課程区分 : 博士後期課程
    開講学部 : 情報科学研究科
    キーワード : 遺伝情報, genetic information, バイオインフォマティクス, bioinformatics, イメージング, imaging, 生体医工学, biomedical engineering, 細胞力学, cell mechanics
  • 応用数学Ⅱ
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 常微分方程式、偏微分方程式、ラプラス変換、フーリエ級数
  • 情報学Ⅰ
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 全学教育
    キーワード : 情報活用,情報社会,情報科学
  • バイオメカニクス基礎
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : DNA、タンパク質、細胞、細胞骨格、細胞膜、骨格・関節、力学、レオロジー、流体、筋出力、キネマトロジー 
  • 生体物理工学
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : DNA、タンパク質、細胞、細胞骨格、細胞膜、骨格・関節、力学、レオロジー、流体、筋出力、キネマトロジー 
  • 生体情報工学実験Ⅱ
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 工学部
    キーワード : 遺伝情報,生体電気現象,生体機能情報,生体計測,バイオエレクトロニクス


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