MF研究者総覧

研究者データベース

詳細
BOMME　GOWDA　SIDDABASAVE　GOWDA(ボメ　ゴウダ　シツダバサーブ　ゴウダ)
保健科学研究院　保健科学部門　健康科学分野
准教授
researchmap個人ページ
Last Updated :2024/05/18

基本情報

通称等の別名

    ゴウダ

所属

  • 保健科学研究院　保健科学部門　健康科学分野

職名

  • 准教授

学位

  • 生命科学における博士号（2016年03月 北海道大学）

連絡先

  • siddabasavegowda.bommegowdahs.hokudai.ac.jp

ホームページURL

科研費研究者番号

  • 50800504

J-Global ID

プロフィール

  • 私は液体クロマトグラフィー質量分析計（LC-MS）を用いて食品や生体システムの研究に10年以上の経験を持つ生物分析化学者です。これまで脂質化学を中心に、その調製、抽出、生化学、分析（リピドミクス）に取り組んできました。私の研究は、食品由来のスフィンゴ脂質（SL）、SL代謝に関連する肥満、およびスフィンゴシン-1-リン酸などの脂質メディエーターを含む分析技術の開発に関するものです。 また、肥満、心筋梗塞、インフルエンザ感染など様々な病態の細胞、マウス、ヒトサンプルにおける脂質バイオマーカーを発見するために、標的および非標的質量分析計の数多くの応用に取り組んでいる。 現在の関心は、脂質（FAHFA：ヒドロキシ脂肪酸の脂肪酸エステル、酸化脂肪酸）や腸内細菌代謝産物（SCFA：短鎖脂肪酸、ヒドロキシ脂肪酸の短鎖脂肪酸エステル（SFAHFA））の新規機能の発見と分析戦略の開発です。また、LC-MSを用いた機能性食品中の未知の脂質や組成の特性評価にも取り組んでいます。



    脂質分析や機能性研究に興味のある臨床医、食品化学者、生物学者との共同研究を楽しみにしています。ご質問やご相談はお気軽にご連絡ください。

研究キーワード

  • 機能性食品と肥満   分析化学   sphingolipids and FAHFAs   LC-MS   lipidomics   脂質化学   食品科学   

研究分野

  • ライフサイエンス / 機能生物化学
  • ライフサイエンス / 生物有機化学
  • 自然科学一般 / 基礎解析学
  • ナノテク・材料 / ケミカルバイオロジー / Lipids synthesis, analysis and applied sciences

担当教育組織

職歴

  • 2023年04月 - 現在 北海道大学 健康科学部 / 大学院国際食資源学院 准教授
  • 2020年10月 - 2023年04月 北海道大学 北海道大学 健康科学部 講師
  • 2022年11月 - 2022年12月 University of South Florida USF Heart Institute
  • 2019年06月 - 2020年09月 北海道大学 北海道大学 健康科学部 Assistant professor
  • 2017年03月 - 2019年04月 RIKEN Center for Integrative Medical Sciences, Japan Postdoctoral Fellow
  • 2016年04月 - 2017年02月 Hokkaido University, Japan Research Fellow
  • 2015年11月 - 2016年03月 Hokkaido University, Japan Research Assitant
  • 2011年10月 - 2012年09月 Yukthi Learning Center, India Assistant professor
  • 2011年06月 - 2011年09月 Akaash IIT-JEE, India Assistant professor
  • 2010年08月 - 2011年05月 Nimbus Tutorials, India Lecture in Chemistry
  • 2009年08月 - 2010年07月 University of Mysore, India Lecture in Chemistry

学歴

  • 2012年10月 - 2016年03月   Hokkaido University   Life Science   PhD
  • 2007年06月 - 2009年07月   University of Mysore   Chemsitry   Master degree

所属学協会

  • 中性脂肪蓄積心筋血管症研究班について   日本臨床化学会   American Society for Mass Spectrometry   Japanese Society for Biomedical Mass Spectrometry   American Chemical Society   International Lipidomics Society   高分子学会   

研究活動情報

論文

  • Lipsa Rani Nath, Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Hitoshi Chiba, Shu Ping Hui
    Food Chemistry 447 138941 - 138941 2024年07月
  • Yusuke Minami, Siddabasave Gowda B Gowda, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui
    Food research international (Ottawa, Ont.) 184 114253 - 114253 2024年05月 
    Sea cucumbers are a rich source of bioactive compounds and are gaining popularity as nutrient-rich seafood. They are consumed as a whole organism in Pacific regions. However, limited data are available on the comparison of their lipid composition and nutritional value. In this study, untargeted liquid chromatography/mass spectrometry was applied to comprehensively profile lipids in the skin, meat, and intestinal contents of three color-distinct edible sea cucumbers. Multivariate principal component analysis revealed that the lipid composition of the intestinal contents of red, black, and blue sea cucumbers differs from that of skin, and meats. Polyunsaturated fatty acids (PUFAs) are abundant in the intestinal contents, followed by meats of sea cucumber. Lipid nutritional quality assessments based on fatty acid composition revealed a high P:S ratio, low index of atherogenicity, and high health promotion indices for the intestinal contents of red sea cucumber, suggesting its potential health benefits. In addition, hierarchical cluster analysis revealed that the intestinal contents of sea cucumbers were relatively high in PUFA-enriched phospholipids and lysophospholipids. Ceramides are abundant in black skin, blue meat, and red intestinal content samples. Overall, this study provides the first insights into a comprehensive regio-specific profile of the lipid content of sea cucumbers and their potential use as a source of lipid nutrients in food and nutraceuticals.
  • Jayashankar Jayaprakash, Siddabasave Gowda B. Gowda, Pradeep K. Shukla, Divyavani Gowda, Lipsa Rani Nath, Hitoshi Chiba, Radhakrishna Rao, Shu-Ping Hui
    ACS Omega 2024年04月09日
  • Natsuki Kita, Asuka Hamamoto, Siddabasave Gowda B. Gowda, Hiroyuki Takatsu, Kazuhisa Nakayama, Makoto Arita, Shu-Ping Hui, Hye-Won Shin
    Journal of Lipid Research 2024年03月
  • Md Abdul Malek, Siddabasave Gowda B. Gowda, Divyavani Gowda, Shu-Ping Hui
    Journal of Food Measurement and Characterization 2024年03月
  • Punith M. Sundaraswamy, Yusuke Minami, Jayashankar Jayaprakash, Siddabasave Gowda B. Gowda, Hiroyuki Takatsu, Divyavani Gowda, Hye-Won Shin, Shu-Ping Hui
    The Analyst 2024年
  • Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Chandra Shekhar, Hitoshi Chiba, Nina Patzke, Shu-Ping Hui
    Heliyon 9 12 e22959 - e22959 2023年12月
  • Divyavani Gowda, Chandra Shekhar, Siddabasave Gowda B. Gowda, Yifan Chen, Shu-Ping Hui
    Livers 3 4 687 - 708 2023年11月23日 
    Non-alcoholic fatty liver disease (NAFLD), a complex liver disorder that can result in non-alcoholic steatohepatitis, cirrhosis, and liver cancer, is the accumulation of fat in the liver seen in people due to metabolic dysfunction. The pathophysiology of NAFLD is influenced by several variables, such as metabolic dysregulation, oxidative stress, inflammation, and genetic susceptibility. This illness seriously threatens global health because of its link to obesity, insulin resistance, type 2 diabetes, and other metabolic disorders. In recent years, lipid–NAFLD crosstalk has drawn a lot of interest. Through numerous methods, lipids have been connected to the onset and advancement of the illness. The connection between lipids and NAFLD is the main topic of the current review, along with the various therapeutic targets and currently available drugs. The importance of hepatic lipid metabolism in the progression of NAFLD is summarized with the latest results in the field.
  • Marumi Ohno, Siddabasave Gowda B Gowda, Toshiki Sekiya, Naoki Nomura, Masashi Shingai, Shu-Ping Hui, Hiroshi Kida
    Scientific reports 13 1 14210 - 14210 2023年08月30日 
    Although influenza virus infection has been shown to affect lipid metabolism, details remain unknown. Therefore, we elucidated the kinetic lipid profiles of mice infected with different doses of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) by measuring multiple lipid molecular species using untargeted lipidomic analysis. C57BL/6 male mice were intranasally infected with PR8 virus at 50 or 500 plaque-forming units to cause sublethal or lethal influenza, respectively. Plasma and tissue samples were collected at 1, 3, and 6 days post-infection (dpi), and comprehensive lipidomic analysis was performed using high-performance liquid chromatography-linear trap quadrupole-Orbitrap mass spectrometry, as well as gene expression analyses. The most prominent feature of the lipid profile in lethally infected mice was the elevated plasma concentrations of phosphatidylethanolamines (PEs) containing polyunsaturated fatty acid (PUFA) at 3 dpi. Furthermore, the facilitation of PUFA-containing phospholipid production in the lungs, but not in the liver, was suggested by gene expression and lipidomic analysis of tissue samples. Given the increased plasma or serum levels of PUFA-containing PEs in patients with other viral infections, especially in severe cases, the elevation of these phospholipids in circulation could be a biomarker of infection and the severity of infectious diseases.
  • Rachana M Gangadhara, Siddabasave Gowda B Gowda, Divyavani Gowda, Ken Inui, Shu-Ping Hui
    Foods (Basel, Switzerland) 12 14 2023年07月21日 
    Beans, a globally significant economic and nutritional food crop, are rich in polyphenolic chemicals with potential health advantages, providing high protein, fiber, minerals, and vitamins. However, studies on the global profiling of lipids in beans are limited. We applied a non-targeted lipidomic approach based on high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (HPLC/LTQ-Orbitrap-MS) to comprehensively profile and compare the lipids in six distinct bean cultivars, namely, adzuki red beans-adzuki cultivar (ARB-AC), adzuki red beans-Benidainagon cultivar (ARB-BC), adzuki red beans-Erimoshouzu cultivar (ARB-EC), soybean-Fukuyutaka cultivar 2021 (SB-FC21), soybean-Fukuyutaka cultivar 2022 (SB-FC22), and soybean-Oosuzu cultivar (SB-OC). MS/MS analysis defined 144 molecular species from four main lipid groups. Multivariate principal component analysis indicated unique lipid compositions in the cultivars except for ARB-BC and ARB-EC. Evaluation of the concentrations of polyunsaturated fatty acid to saturated fatty acid ratio among all the cultivars showed that SB-FC21 and SB-FC22 had the highest value, suggesting they are the most beneficial for health. Furthermore, lipids such as acyl sterol glycosides were detected and characterized for the first time in these bean cultivars. Hierarchical cluster correlations revealed the predominance of ceramides in ARB-EC, lysophospholipids in SB-FC21, and glycerophospholipids in SB-OC. This study comprehensively investigated lipids and their compositions in beans, indicating their potential utility in the nutritional evaluation of beans as functional foods.
  • Ganesh V Halade, Yusuf Mat, Siddabasave Gowda B Gowda, Shalini Jain, Shu-Ping Hui, Hariom Yadav, Vasundhara Kain
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37 5 e22899  2023年05月 
    Sleep is a fundamental medicine for cardiac homeostasis, and sleep-deprived individuals are prone to higher incidences of heart attack. The lipid-dense diet (obesogenic diet-OBD) is a cumulative risk factor for chronic inflammation in cardiovascular disease; thus, understanding how sleep fragmentation (SF) in an obesity setting impacts immune and cardiac health is an unmet medical need. We hypothesized whether the co-existence of SF with OBD dysregulates gut homeostasis and leukocyte-derived reparative/resolution mediators, thereby impairing cardiac repair. Two-month-old male C57BL/6J mice were randomized first into two groups, then four groups; Control, control + SF, OBD, and OBD + SF mice subjected to myocardial infarction (MI). OBD mice had higher levels of plasma linolenic acid with a decrease in eicosapentaenoic and docosahexaenoic acid. The OBD mice had lower Lactobacillus johnsonii indicating a loss of probiotic microbiota. SF in OBD mice increased Firmicutes/Bacteroidetes ratio indicative of a detrimental change in SF-directed microbiome. OBD + SF group increased in the neutrophil: lymphocyte ratio suggestive of suboptimal inflammation. As a result of SF, resolution mediators (RvD2, RvD3, RvD5, LXA4 , PD1, and MaR1) decreased and inflammatory mediators (PGD2 , PGE2 , PGF2a , 6k-PGF1a ) were increased in OBD mice post-MI. At the site of infarction, the proinflammatory cytokines Ccl2, IL1β, and IL-6 were amplified in OBD + SF indicating a robust proinflammatory milieu post-MI. Also, brain circadian genes (Bmal1, Clock) were downregulated in SF-subjected control mice, but remained elevated in OBD mice post-MI. SF superimposed on obesity dysregulated physiological inflammation and disrupted resolving response thereby impaired cardiac repair and signs of pathological inflammation.
  • Siddabasave Gowda B Gowda, Chandra Shekhar, Divyavani Gowda, Yifan Chen, Hitoshi Chiba, Shu-Ping Hui
    Mass spectrometry reviews 2023年04月27日 
    Coronavirus disease 2019 (COVID-19) has emerged as a global health threat and has rapidly spread worldwide. Significant changes in the lipid profile before and after COVID-19 confirmed the significance of lipid metabolism in regulating the response to viral infection. Therefore, understanding the role of lipid metabolism may facilitate the development of new therapeutics for COVID-19. Owing to their high sensitivity and accuracy, mass spectrometry (MS)-based methods are widely used for rapidly identifying and quantifying of thousands of lipid species present in a small amount of sample. To enhance the capabilities of MS for the qualitative and quantitative analysis of lipids, different platforms have been combined to cover a wide range of lipidomes with high sensitivity, specificity, and accuracy. Currently, MS-based technologies are being established as efficient methods for discovering potential diagnostic biomarkers for COVID-19 and related diseases. As the lipidome of the host cell is drastically affected by the viral replication process, investigating lipid profile alterations in patients with COVID-19 and targeting lipid metabolism pathways are considered to be crucial steps in host-directed drug targeting to develop better therapeutic strategies. This review summarizes various MS-based strategies that have been developed for lipidomic analyzes and biomarker discoveries to combat COVID-19 by integrating various other potential approaches using different human samples. Furthermore, this review discusses the challenges in using MS technologies and future perspectives in terms of drug discovery and diagnosis of COVID-19.
  • Asuka Hamamoto, Natsuki Kita, Siddabasave Gowda B Gowda, Hiroyuki Takatsu, Kazuhisa Nakayama, Makoto Arita, Shu-Ping Hui, Hye-Won Shin
    Cell Structure and Function 2023年
  • Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Hitoshi Chiba, Vibhu Parcha, Pankaj Arora, Ganesh V. Halade, Shu-Ping Hui
    Atherosclerosis 363 30 - 41 2022年12月
  • Siddabasave Gowda B. Gowda, Yusuke Minami, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui
    Food Chemistry 393 133402 - 133402 2022年11月
  • Siddabasave Gowda B. Gowda, Chen Yifan, Divyavani Gowda, Yui Tsuboi, Hitoshi Chiba, Shu-Ping Hui
    Antioxidants 11 8 1538 - 1538 2022年08月08日 
    Seaweeds are a good source of bioactive lipids and are known for their nutritional benefits, making them a valuable food source. Despite their dietary significance and nutritional importance, there are limited reports on comprehensive lipidome analysis of lipids with antioxidant properties. Therefore, this study aimed to compare the lipid profiles of five commonly consumed Japanese dietary seaweeds using non-targeted liquid chromatography/mass spectrometry (LC/MS). A total, of 304 molecular species from four major lipid classes were detected and characterized by MS/MS analysis. Multivariate statistical analysis revealed distinct lipid molecular compositions in kombu and sea mustard compared to hijiki, mozuku, and laver seaweeds. Kombu has been shown to contain large amounts of antioxidants, such as polyunsaturated fatty acids (PUFAs), and a high health promotion index compared to other seaweeds. Hierarchical cluster correlations indicated the predominance of glycerophospholipids (GPs) and glycerolipids (GLs) in sea mustard and kombu. As a result, dietary seaweeds have great potential as antioxidants and health-promoting foods for human consumption due to their high levels of PUFA-rich GPs and GLs. Unsaturated triacylglycerols are predominant in hijiki, whereas other health-beneficial lipids, such as monogalactosyldiacylglycerol and sulfoquinovosyl diacylglycerols, are predominant in sea mustard. This study provides a detailed characterization of lipids and their comparative fingerprints in seaweeds, demonstrating the potential use of dietary seaweeds in biotechnological and industrial applications involving the development of functional food products.
  • Divyavani Gowda, Yonghan Li, Siddabasave Gowda B Gowda, Marumi Ohno, Hitoshi Chiba, Shu-Ping Hui
    Analytical and bioanalytical chemistry 414 22 6419 - 6430 2022年07月16日 
    Short-chain fatty acids (SCFAs) are the end products of the fermentation of complex carbohydrates by the gut microbiota. Although SCFAs are recognized as important markers to elucidate the link between gut health and disease, it has been difficult to analyze SCFAs with mass spectrometry technologies due to their poor ionization efficiency and high volatility. Here, we present a novel and sensitive method for the quantification of SCFAs, including C2-C6 SCFAs and their hydroxy derivatives, by liquid chromatography/tandem mass spectrometry (LC-MS/MS) upon N,N-dimethylethylenediamine (DMED) derivatization with a run time of 10 min. Moreover, the quantification method of DMED-derivatized SCFAs in intestinal contents using isotope-labeled internal standards was also established. The method validation was performed by analyzing spiked intestinal samples; the limits of detection and quantification of SCFAs with this method were found to be 0.5 and 5 fmol, respectively; the recovery was greater than 80% and good linearity (0.9932 to 0.9979) of calibration curves was obtained over the range from 0.005 to 5000 pmol/μL; the intraday and interday precisions were achieved in the range of 1-5%. Furthermore, the validated method was applied to analyze SCFAs in the cecum and colon contents of mice infected with the influenza virus. The results showed that the concentration of most of the SCFAs tested here decreased significantly in a time-dependent manner after the infection, suggesting a possibility that SCFAs in intestinal samples could be used as severe disease markers. Overall, we here successfully developed a simple, fast, and sensitive method for SCFA analysis by LC-MS/MS combined with DMED derivatization. The method for the quantification of SCFAs will be a useful tool for both basic research and clinical studies.
  • S. G. B. Gowda, Y. Sasaki, E. Hasegawa, H. Chiba, S. P. Hui
    Journal of Insects as Food and Feed 8 2 157 - 168 2022年02月01日 [査読有り]
     
    Insects such as Tenebrio molitor have been considered an alternative source of nutrition for animals and have also been adopted as human food throughout history, especially in Asia and Africa. Lipids are the second most abundant component followed by proteins. However, studies focusing on comprehensive lipid composition analsysis of these widely reared species are limited. The untargeted lipidomic analysis of yellow mealworm larvae (T. molitor) led to the identification of several lipid molecular species from lipid classes such as: free fatty acids, sphingolipids, phospholipids, and triacylglycerols. The results revealed that polyunsaturated fatty acids (PUFAs) (45%) are the most abundant fatty acids, followed by monounsaturated fatty acids (MUFAs) (42%) and saturated fatty acids (13%). Fatty acids such as FA 18:1 and FA 18:2 are the most abundant fatty acids and are substantially enriched in other complex lipids in the form of esters. Moreover, functional lipids such as sphingomyelins, ceramides, cardiolipins, phosphatidylinositols, and phosphatidylethanolamines were characterised for the first time, with a large number of MUFAs and PUFAs as their main acyl chains. Overall, our data showed the occurrence of multiple structurally diverse lipids in T. molitor, suggesting that mealworms are not only enriched with proteins but also have several functional lipids, which are highly beneficial to human and animal health. Thus, the larvae of T. molitor could serve as a promising candidate for the development of functional food and feed products.
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  • Yoshimi Miki, Yoshitaka Taketomi, Yuh Kidoguchi, Kei Yamamoto, Kazuaki Muramatsu, Yasumasa Nishito, Jonguk Park, Koji Hosomi, Kenji Mizuguchi, Jun Kunisawa, Tomoyoshi Soga, Eric Boilard, Siddabasave Gowda B. Gowda, Kazutaka Ikeda, Makoto Arita, Makoto Murakami
    JCI Insight 7 2 2022年01月25日 
    Besides promoting inflammation by mobilizing lipid mediators, group IIA secreted phospholipase A2 (sPLA2-IIA) prevents bacterial infection by degrading bacterial membranes. Here, we show that, despite the restricted intestinal expression of sPLA2-IIA in BALB/c mice, its genetic deletion leads to amelioration of cancer and exacerbation of psoriasis in distal skin. Intestinal expression of sPLA2-IIA is reduced after treatment with antibiotics or under germ-free conditions, suggesting its upregulation by gut microbiota. Metagenome, transcriptome, and metabolome analyses have revealed that sPLA2-IIA deficiency alters the gut microbiota, accompanied by notable changes in the intestinal expression of genes related to immunity and metabolism, as well as in the levels of various blood metabolites and fecal bacterial lipids, suggesting that sPLA2-IIA contributes to shaping of the gut microbiota. The skin phenotypes in Pla2g2a-/- mice are lost (a) when they are cohoused with littermate WT mice, resulting in the mixing of the microbiota between the genotypes, or (b) when they are housed in a more stringent pathogen-free facility, where Pla2g2a expression in WT mice is low and the gut microbial compositions in both genotypes are nearly identical. Thus, our results highlight a potentially new aspect of sPLA2-IIA as a modulator of gut microbiota, perturbation of which affects distal skin responses.
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  • Chongsheng Liang, Siddabasave Gowda B. Gowda, Divyavani Gowda, Toshihiro Sakurai, Iku Sazaki, Hitoshi Chiba, Shu-Ping Hui
    Antioxidants 11 2 229 - 229 2022年01月25日 
    Lipid hydroperoxides (LOOH) are the initial products of the peroxidation of unsaturated lipids and play a crucial role in lipid oxidation due to their ability to decompose into free radicals and cause adverse effects on human health. Thus, LOOHs are commonly considered biomarkers of oxidative stress-associated pathological conditions. Despite their importance, the sensitive and selective analytical method for determination is limited, due to their low abundance, poor stability, and low ionizing efficiency. To overcome these limitations, in this study, we chemically synthesized eight fatty acid hydroperoxides (FAOOH), including FA 18:1-OOH, FA 18:2-OOH, FA 18:3-OOH, FA 20:4-OOH, FA 20:5-OOH, FA 22:1-OOH, FA 22:6-OOH as analytes, and FA 19:1-OOH as internal standard. Then, they were chemically labeled with 2-methoxypropene (2-MxP) to obtain FAOOMxP by one-step derivatization (for 10 min). A selected reaction monitoring assisted targeted analytical method was developed using liquid chromatography/tandem mass spectrometry (LC-MS/MS). The MxP-labelling improved the stability and enhanced the ionization efficiency in positive mode. Application of reverse-phase chromatography allowed coelution of analytes and internal standards with a short analysis time of 6 min. The limit of detection and quantification for FAOOH ranged from 0.1–1 pmol/µL and 1–2.5 pmol/µL, respectively. The method was applied to profile total FAOOHs in chemically oxidized human serum samples (n = 5) and their fractions of low and high-density lipoproteins (n = 4). The linoleic acid hydroperoxide (FA 18:2-OOH) and oleic acid hydroperoxide (FA 18:1-OOH) were the most abundant FAOOHs in human serum and lipoproteins. Overall, our validated LC-MS/MS methodology features enhanced detection and rapid separation that enables facile quantitation of multiple FAOOHs, therefore providing a valuable tool for determining the level of lipid peroxidation with potential diagnostic applications.
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  • Divyavani Gowda, Marumi Ohno, Siddabasave Gowda B. Gowda, Hitoshi Chiba, Masashi Shingai, Hiroshi Kida, Shu-Ping Hui
    Scientific Reports 11 1 20161 - 20161 2021年12月 
    AbstractInfluenza remains a world-wide health concern, causing 290,000–600,000 deaths and up to 5 million cases of severe illnesses annually. Noticing the host factors that control biological responses, such as inflammatory cytokine secretion, to influenza virus infection is important for the development of novel drugs. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite and has essential biological functions in inflammation. However, the kinetic effects of influenza virus infection on physiological S1P levels and their signaling in multiple tissues remain unknown. In this study, we utilized a mouse model intranasally infected with 50 or 500 plaque forming units (PFU) of A/Puerto Rico/8/34 (H1N1; PR8) virus to investigate how S1P levels and expression of its regulating factors are affected by influenza virus infection by the liquid-chromatography/mass spectrometry and real-time PCR, respectively. The S1P level was significantly high in the plasma of mice infected with 500 PFU of the virus than that in control mice at 6 day-post-infection (dpi). Elevated gene expression of sphingosine kinase-1 (Sphk1), an S1P synthase, was observed in the liver, lung, white adipose tissue, heart, and aorta of infected mice. This could be responsible for the increased plasma S1P levels as well as the decrease in the hepatic S1P lyase (Sgpl1) gene in the infected mice. These results indicate modulation of S1P-signaling by influenza virus infection. Since S1P regulates inflammation and leukocyte migration, it must be worth trying to target this signaling to control influenza-associated symptoms.
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  • SiddabasaveGowda B. Gowda, Divyavani Gowda, Vasundhara Kain, Hitoshi Chiba, Shu-Ping Hui, Charles E. Chalfant, Vibhu Parcha, Pankaj Arora, Ganesh V. Halade
    American Journal of Physiology-Heart and Circulatory Physiology 321 3 H599 - H611 2021年09月01日 [査読有り][通常論文]
     
    Previous studies indicate that sphingosine-1-phosphate (S1P) has some role in cardiovascular disease. This study adds quantitative and integrative systems-based approaches that are necessary for discovery and bedside translation. Here, we quantitated sphinganine, sphingosine, sphingosine-1-phosphate (S1P) in mice and human cardiac pathobiology. Interorgan S1P quantity and respective systems-based receptor activation suggest cardiac repair after myocardial infarction. Thus, S1P serves as a therapeutic target for cardiac protection in clinical translation.
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  • Siddabasave Gowda B. Gowda, Takayuki Tsukui, Hirotoshi Fuda, Yusuke Minami, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui
    International Journal of Molecular Sciences 22 14 7598 - 7598 2021年07月15日 [査読有り]
     
    Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with interesting physiological functions in mammals. Despite their structural diversity and links with nuclear factor erythroid 2-related factor 2 (NRF2) biosynthesis, FAHFAs are less explored as NRF2 activators. Herein, we examined for the first time the synthetic docosahexaenoic acid esters of 12-hydroxy stearic acid (12-DHAHSA) or oleic acid (12-DHAHOA) against NRF2 activation in cultured human hepatoma-derived cells (C3A). The effect of DHA-derived FAHFAs on lipid metabolism was explored by the nontargeted lipidomic analysis using liquid chromatography-mass spectrometry. Furthermore, their action on lipid droplet (LD) oxidation was investigated by the fluorescence imaging technique. The DHA-derived FAHFAs showed less cytotoxicity compared to their native fatty acids and activated the NRF2 in a dose-dependent pattern. Treatment of 12-DHAHOA with C3A cells upregulated the cellular triacylglycerol levels by 17-fold compared to the untreated group. Fluorescence imaging analysis also revealed the suppression of the degree of LDs oxidation upon treatment with 12-DHAHSA. Overall, these results suggest that DHA-derived FAHFAs as novel and potent activators of NRF2 with plausible antioxidant function.
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  • Siddabasave Gowda B. Gowda, Divyavani Gowda, Marumi Ohno, Chongsheng Liang, Hitoshi Chiba, Shu-Ping Hui
    Journal of the American Society for Mass Spectrometry 32 8 2196 - 2205 2021年06月25日 [査読有り][通常論文]
     
    Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with promising physiological functions in mammals. We previously introduced a new type of lipids to this family called short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs), branching specific to the C2 carbon of a long-chain fatty acid (≥C20). In this study, we discovered a homologous series of SFAHFAs comprising C16-C26 hydroxy fatty acids esterified with short-chain fatty acids (C2-C5) in mouse colon contents. The detected SFAHFAs were characterized by high-resolution mass spectrometry with MSn analysis. The double-bond position of monounsaturated SFAHFAs was determined by the epoxidation reaction of samples with m-chloroperoxybenzoic acid and their MSn analysis. Further, the measurement of SFAHFA concentration in the colon contents of mice infected with influenza A/Puerto Rico/8/34 (H1N1; PR8) virus revealed a significant increase in their levels compared to native control. A strong correlation was observed between hydroxy fatty acid and SFAHFAs. Detection, characterization, and profiling of these new SFAHFA levels in relation with pandemic H1N1; PR8 influenza virus will contribute to the in-depth study of their function and metabolism.
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  • Siddabasave Gowda B. Gowda, Yusuke Minami, Divyavani Gowda, Daisuke Furuko, Hitoshi Chiba, Shu-Ping Hui
    Food Research International 144 110325 - 110325 2021年03月 [査読有り][通常論文]
     
    Lipids such as furan fatty acids (F-acids) are the valuable minor bioactive components of food such as fatty fish and plants. They are reported to have positive health benefits, including antioxidant and anti-inflammatory activities. Despite their importance, limited studies are focusing on F-acid determination in dietary seafood. This study aimed to identify and profile non-esterified F-acids and free fatty acids in total lipid extract of seafood such as shellfish and salmon. The lipidomic analysis using liquid chromatography-linear trap quadrupole-orbitrap mass spectrometry led to identifying seven types of free F-acids in shellfish (n = 5) and salmon (n = 4). The identified F-acids were confirmed by their high-resolution masses and acquired mass spectra. The relative concentrations of F-acids in shellfish range from 0.01 to 10.93 mg/100 g of the fillet, and in salmon, 0.01 to 14.21 mg/100 g of the fillet. The results revealed the highest abundance of F-acids in Sakhalin surf clam, Japanese scallop, and a fatty salmon trout. Besides, relative levels of saturated, monounsaturated, and polyunsaturated fatty acids (PUFAs) in these seafoods were compared with each other, suggesting basket clams and salmon trout to have significantly higher levels of PUFAs. The dietary seafoods enriched with F-acids and PUFAs may have possible health benefits. Hence, the applied technique could be a promising tool for rapid detection and analysis of non-esterified fatty acids in food.
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  • Yusuke Yamamoto, Toshihiro Sakurai, Zhen Chen, Takayuki Furukawa, Siddabasave Gowda, B. Gowda, Yue Wu, Kazuhiro Nouso, Yuki Fujii, Yuki Yoshikawa, Hitoshi Chiba, Shu-Ping Hui
    Analytical and Bioanalytical Chemistry 2020年10月22日 [査読有り][通常論文]
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  • Siddabasave Gowda B. Gowda, Divyavani Gowda, Chongsheng Liang, Yonghan Li, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, Shu-Ping Hui
    Metabolites 10 10 398 - 398 2020年10月08日 [査読有り][通常論文]
     
    Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.
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  • Siddabasave Gowda B. Gowda, Chongsheng Liang, Divyavani Gowda, Fengjue Hou, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, Shu‐Ping Hui
    Rapid Communications in Mass Spectrometry 34 17 2020年09月15日 [査読有り][通常論文]
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  • Siddabasave Gowda B. GOWDA, Zi-Jun GAO, Zhen CHEN, Takayuki ABE, Shota HORI, Satoru FUKIYA, Satoshi ISHIZUKA, Atsushi YOKOTA, Hitoshi CHIBA, Shu-Ping HUI
    Analytical Sciences 36 7 821 - 828 2020年07月10日 [査読有り][通常論文]
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  • Siddabasave Gowda B. Gowda, Hirotoshi Fuda, Yusuke Yamamoto, Hitoshi Chiba, Shu‐Ping Hui
    Lipids 55 4 395 - 401 2020年07月 [査読有り][通常論文]
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  • Siddabasave Gowda B. Gowda, Hirotoshi Fuda, Takayuki Tsukui, Hitoshi Chiba, Shu-Ping Hui
    Antioxidants 9 5 397 - 397 2020年05月08日 [査読有り][通常論文]
     
    Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biologically active lipids with anti-inflammatory and anti-diabetic properties. Despite the possible link between endogenous FAHFA levels and nuclear factor erythroid 2-related factor 2 (Nrf2), their possible function as antioxidants and the mechanisms involved in this are unknown. Here, we investigate FAHFAs’ plausible antioxidant potential with reference to their effect on the Nrf2 levels, oxidative stress, and lipid droplet oxidation in human hepatocytes (C3A). Six authentic FAHFAs were chemically synthesized and performed activity-based screening by reporter gene assay. Among them, eicosapentaenoic acid (EPA) esterified 12-hydroxy stearic acid (12-HSA) and 12-hydroxy oleic acid (12-HOA) FAHFAs showed less cytotoxicity compared to their free fatty acids and potent activators of Nrf2. To define their mode of action, relative levels of nuclear Nrf2 were determined, which found a higher amount of Nrf2 in nucleus of cells treated with 12-EPAHSA compared to the control. Furthermore, 12-EPAHSA increased the expression of Nrf2-dependent antioxidant enzyme genes (NQO1, GCLM, GCLC, SOD-1, and HO-1). Fluorescence imaging analysis of linoleic-acid-induced lipid droplets (LDs) in C3A cells treated with 12-EPAHSA revealed the strong inhibition of small-size LD oxidation. These results suggest that EPA-derived FAHFAs as a new class of lipids with less cytotoxicity, and strong Nrf2 activators with plausible antioxidant effects via the induction of cytoprotective proteins against oxidative stress, induced cellular damage.
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  • Siddabasave Gowda B Gowda, Atsufumi Nakahashi, Keiko Yamane, Saori Nakahashi, Yuta Murai, Ananda Kumar C Siddegowda, Mostafa A S Hammam, Kenji Monde
    ACS omega 3 1 753 - 759 2018年01月31日 [査読有り][通常論文]
     
    Sphingoid bases, which have a 2-amino-1,3-diol common functional group, are the structural backbone units of all sphingolipids. Recently, much attention has been focused on sphingoid bases because of their potentially beneficial bioactivities toward various cancer cells as well as their dietary interest. However, low abundance and the handling complexity caused by their amphiphilic character led to very limited research on them. Glutaraldehyde has two aldehyde groups, and it reacts rapidly with the 2-amino-1,3-diol functional group of sphingosine to give a tricyclic product. Immobilization of glutaraldehyde on a resin was successfully performed by organic synthesis, starting from trans-p-coumaric acid via eight steps. This approach suppresses the self-polymerization of glutaraldehyde, and addition of water to the developed resin causes the formation of cyclic double hemiacetal function, which avoids oxidation like a reducing sugar in nature and makes it stable even for up to 1 year incubation. The resin was applied to the solid-phase extracting experiment of free sphingosine from human serum at a concentration of 280 nM. Another extraction study of edible golden oyster mushrooms showed that the sphingoid base was selectively captured from complex natural extracts. These results demonstrate that the developed glutaraldehyde resin method is a highly selective method, and hence, the combination of it with the o-phthaldialdehyde HPLC method was confirmed as an efficient and sensitive method for analysis of sphingoid bases in biological samples.
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  • ボメゴウダシッダバサヴゴウダ
    Analytical Bioanalytical Chemistry 410 20 4793 - 4803 2018年 [査読有り][通常論文]
     
    Extraction and analysis of sphingolipids from biological samples is a critical step in lipidomics, especially for minor species such as sphingoid bases and sphingosine-1-phosphate. Although several liquid chromatography-mass spectrometry methods enabling the determination of sphingolipid molecular species have been reported, they were limited in analytical sensitivity and reproducibility by causing significant peak tailing, especially by the presence of phosphate groups, and most of the extraction techniques are laborious and do not cover a broad range of sphingolipid metabolites. In this study, we developed a rapid single-phase extraction and highly sensitive analytical method for the detection and quantification of sphingolipids (including phosphates) comprehensively using liquid chromatography-triple quadruple mass spectrometry. After validating the reliability of the method, we analyzed the intestinal tissue sphingolipids of germ-free (GF) and specific pathogen-free (SPF) mice and found significantly higher levels of free sphingoid bases and sphingosine-1-phosphate in the GF condition as compared to the SPF condition. This method enables a rapid extraction and highly sensitive determination of sphingolipids comprehensively at low femtomolar ranges. Graphical abstract Diagrammatic comparision of sphingolipid (phosphates) analysis between conventional and this method.
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  • Atsufumi Nakahashi, Ananda Kumar C. Siddegowda, Mostafa A. S. Hammam, Siddabasave Gowda B. Gowda, Yuta Murai, Kenji Monde
    ORGANIC LETTERS 18 10 2327 - 2330 2016年05月 [査読有り][通常論文]
     
    Vibrational circular dichroism (VCD) was first applied to the stereochemical analysis of sphingosine. VCD patterns derived from the C=C stretch as well as other mid-infrared (IR) regions were practical markers to discriminate all the stereoisomers of intact sphingosine. Glutaraldehyde was found as an excellent derivatizing reagent for sphingosine which improves its solubility in VCD-friendly nonpolar solvents such as chloroform and enhances the VCD intensities by forming a rigid cyclized structure.
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  • Siddabasave B. Gowda Gowda, Seigo Usuki, Mostafa A. S. Hammam, Yuta Murai, Yasuyuki Igarashi, Kenji Monde
    JOURNAL OF LIPID RESEARCH 57 2 325 - 331 2016年02月 [査読有り][通常論文]
     
    Sphingoid base derivatives have attracted increasing attention as promising chemotherapeutic candidates against lifestyle diseases such as diabetes and cancer. Natural sphingoid bases can be a potential resource instead of those derived by time-consuming total organic synthesis. In particular, glucosylceramides (GlcCers) in food plants are enriched sources of sphingoid bases, differing from those of animals. Several chemical methodologies to transform GlcCers to sphingoid bases have already investigated; however, these conventional methods using acid or alkaline hydrolysis are not efficient due to poor reaction yield, producing complex by-products and resulting in separation problems. In this study, an extremely efficient and practical chemoenzymatic transformation method has been developed using microwave-enhanced butanolysis of GlcCers and a large amount of readily available almond beta-glucosidase for its deglycosylation reaction of lysoGlcCers. The method is superior to conventional acid/base hydrolysis methods in its rapidity and its reaction cleanness (no isomerization, no rearrangement) with excellent overall yield.

書籍

  • 環境・エネルギーハーベスティングのためのナノコンポジット材料の進歩
    (担当:共著範囲:カーボンナノ構造体ベースの環境・エネルギー応用複合材料)
    スプリンガー・ネイチャー 2022年 (ISBN: 9783030943196)

講演・口頭発表等

  • マウスおよび虚血性心疾患患者における急性期から慢性期までの脂質動態。
    Siddabasave Gowda, B. Gowda, Gowda D, Chiba H, Parcha V, Arora P, Halade G, Hui S-P
    第17回がん・炎症・関連疾患における生理活性脂質、2022年10月30日-11月02日、米国ニューオリンズ。
  • ヒドロキシ脂肪酸の短鎖脂肪酸エステル類の簡便な分析法
    Fengiue H, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
    17th Bioactive lipids in Cancer, Inflammation and Related Diseases, Oct-30- Nov. 02, 2022, New Orleans, USA.
  • カソケロウィルス感染による脂質代謝への影響に関する検討
    Siddabasave Gowda, B. Gowda, Kajihara M, Ohno M
    The 8th Hokkaido University Cross-Departmental Symposium, 2022, Oct 28, Sapporo,
  • LC/MSによる心不全の脂質バイオマーカーの解析
    Siddabasave gowda, B. Gowda
    第５回学術集会 / 第２回中性脂肪月間, 2022, Oct 15, 福岡、日本
  • 肥満症は世界的に懸念されている生活習慣病  [招待講演]
    Siddabasave Gowda, B. Gowda
    第2回国際会議 国連世界平和協会2022、 口頭発表（一般）
  • 食用海藻5種における脂質の特性解析について 液体クロマトグラフィー/質量分析法
    Chen Y, Hui S-P, Chiba H, D, Gowda, Siddabasave Gowda, B. Gowda
    61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
  • ヒドロキシ脂肪酸の短鎖脂肪酸エステルの液体クロマトグラフィー/質量分析による解析 クロマトグラフィー／質量分析計による分析
    Li Y, Siddabasave Gowda, B. Gowda, D, Gowda, Hui S-P, Chiba H
    61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
  • インフルエンザウイルスA / PR8 / 34 (H1N1)のスフィンゴシン-1-リン酸シグナルに対する をターゲットとしたLC / MSによるマウスでの解析
    D., Gowda, Chiba H, Hui S-P, Siddabasave Gowda, B. Gowda
    61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
  • "液体クロマトグラフィー質量分析法によるインフルエンザウイルス感染症の脂質バイオマーカー解析"
    Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
    61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
  • “肥満に関連するスフィンゴ脂質代謝に対する食品由来阻害剤の発見“.
    Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
    61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
  • ヒドロキシ脂肪酸の短鎖脂肪酸エステル" の簡便な分析法の開発  [通常講演]
    Hou F, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
    第47回日本医用マススペクトル学会年会. 2022, Sept 9-10, 東京, 日本.
  • 食用魚11品目の未知脂質に挑むと同時に、その組成をアンターゲットLC/MSで解析 アンターゲットLC/MSによる組成分析 "
    Siddabasave Gowda, B. Gowda, Minami Y, D., Gowda, Chiba H, Hui S-P
    第47回日本医用マススペクトル学会年会. 2022, Sept 9-10, 東京, 日本. 口頭発表（一般）
  • "食品由来の肥満関連スフィンゴ脂質代謝阻害剤の発見"  [通常講演]
    Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
    第47回日本医用マススペクトル学会年会. 2022, Sept 9-10, 東京, 日本. ポスター発表
  • アンターゲットLC/MSによる11種類の食用魚の脂質フィンガープリント法  [招待講演]
    Siddabasave Gowda, B. Gowda, Minami Y, Gowda D, Chiba H, Hui S-P
    第6回国際シンポジウム「医療と食品におけるファイトケミカル」開催 2022年08月 口頭発表（一般）
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  • Exploring Functional Lipids in Food, Health, and Disease by state-of-the-art of mass spectrometry
    Siddabasave gowda, Bomme Gowda
    Two days Virtual International Conference on Advanced Materials, Dept. of Organic Chemistry, University of Mysore, India. 2022年03月 口頭発表（招待・特別）
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  • 急性心筋梗塞から慢性心筋梗塞までの脂質のキネティックプロファイル
    Siddabasave Gowda, B. Gowda, Gowda D, Chiba H, Hui S-P
    第61回日本臨床化学会年次学術集会.(online) 2021,東京, 日本. 2021年11月 口頭発表（一般）
  • 健康・疾病における機能性脂質の化学  [招待講演]
    Siddabasave gowda, Bomme Gowda
    A Virtual Conference on Recent Advances in Chemical Science and Medicinal Chemistry,JSS Science & Technology University, India 2021年07月 口頭発表（招待・特別）
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  • LC-MS/MSを用いたワンステップ誘導体化による脂質ヒドロペルオキシドの測定
    Chongsheng Liang, Siddabasavegowda, B. Gowda, Hitoshi Chiba, Shu-ping Hui
    第60回日本臨床化学会年次学術集会 ポスター発表
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  • 心筋梗塞におけるS1Pシグナリングの役割
    Siddabasave Gowda, B. Gowda, Divayvani Gowda, Hitoshi Chiba, Ganesh Halade, Shu-ping Hui
    第60回日本臨床化学会年次学術集会 ポスター発表
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  • "Nrf2を介した強力な抗酸化物質としてのn-3系脂肪酸由来の脂質の発見"
    Hitoshi Chiba, Siddabasavegowda, B. Gowda, Hirotoshi Fuda, Takayuki Tsukui, Shu-Ping Hui
    第60回日本臨床化学会年次学術集会 口頭発表（一般）
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  • Global lipid fingerprinting for biomarker discovery and their functional studies
    Siddabasave gowda, B. Gowda
    第6回 北大・部局横断シンポジウム 口頭発表（一般）
  • リピドミクスによる健 康と病気における脂質 の役割の解明
    Siddabasave Gowda, B. Gowda
    超異分野meetup- 2020 Sapporo, Japan (札幌，日本) ポスター発表
  • Improved method for analysis of sphingolipids by ultra-high performance liquid chromatography- mass spectrometry”2nd Japan-Korea Lipid symposium  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    2nd Japan-Korea Lipid symposium 2018年09月 口頭発表（一般）
  • Application of novel polymer sustained glutaraldehyde resin towards rapid extraction of sphigolipids  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    Japan-Taiwan Bilateral polymer symposium 2015年09月 ポスター発表
  • A novel approach towards efficient supply of natural sphingoid bases and their application as multi drug resistance inhibitors in cancer  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    14th International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases 2015年07月 ポスター発表
  • Development of novel extraction method of natural sphingoids by chemoselective capturing reaction  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    27th Banyu Sapporo Symposium 2015年07月 ポスター発表
  • Application of novel polymer sustained glutaraldehyde resin towards rapid extraction of sphingoids  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    64th SPSJ Annual Meeting 2015年05月 ポスター発表
  • Innovative tactic towards rapid isolation of unique bioactive sphingoids from dietary natural resources  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    249th ACS National Meeting & Exposition 2015年03月 口頭発表（一般）
  • Development of a highly efficient novel methodology for the rapid isolation of bioactive sphingoid base – toward construction of their chemical library  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    The 2nd International Life-Science Symposium for Young Scientists 2014年10月 口頭発表（一般）
  • Development of a highly efficient novel methodology for the isolation of sphingoid bases and its application towards construction of their chemical library  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    The 12th International Symposium for future Drug Discovery and Medical Care 2014年09月 ポスター発表
  • Chemoselective capture of sphingolipids by efficient resin and stereochemical analysis method  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    Molecular Chirality 2014 2014年06月 ポスター発表
  • A new tactic towards rapid extraction of unique sphingoid bases  [通常講演]
    ボメゴウダシッダバサヴゴウダ
    Chemical Society Japan Meeting 2014年01月 口頭発表（一般）
  • ヒドロキシ脂肪酸の短鎖脂肪酸エステル - 肥満とインフルエンザ感染の新しい腸内脂質バイオマーカー
    SIDDABASAVEGOWDA B. GOWDA, CHONGSHENG LIANG, DIVYAVANI GOWDA, FENGJUE HOU, HITOSHI CHIBU, SHU-PING HUI
    口頭発表（一般）
  • 化学的標識による短鎖脂肪酸エステルの検出と同定の支援 ラット結腸および盲腸内容物中のヒドロキシ脂肪酸の脂肪酸エステルの検出および同定の補助
    i Y, D, Gowda, Siddabasave Gowda, B. Gowda, Liang C, Chiba H, Hui S-P
    第61回日本臨床化学会年次学術集会". 2021, 福岡、日本.
  • LC/MSによる早期疾患バイオマーカー探索のためのグローバル脂質フィンガープリンティング
    第46回日本医用マススペクトル学会年会. 2021,宮城, 日本. 口頭発表（招待・特別）
  • 白血病における薬剤耐性の脂質バイオマーカー探索のための脂質フィンガープリンティング
    Matsuhisa C, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S.P
    第46回日本医用マススペクトル学会年会. 2021,宮城, 日本. 口頭発表（一般）
  • UHPLC/LTQ Orbitrap MSを用いた食餌性日本産魚類の脂質組成の網羅的解析
    Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
    第46回日本医用マススペクトル学会年会. 2021,宮城, 日本. 口頭発表（一般）
  • ヒドロキシ脂肪酸の短鎖脂肪酸エステルの合成と簡便な分析法の開発
    Fengiue H, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
    第46回日本医用マススペクトル学会年会. 2021,宮城,日本. 口頭発表（一般）
  • LC/MSを用いた脂肪酸ヒドロペルオキシドのワンステップ誘導体化によるターゲット分析  [通常講演]
    Liang C, Siddabasave Gowda B, Sakurai T, Chiba H, Hui S.P
    第46回日本医用マススペクトル学会年会. 2021,宮城, 日本 口頭発表（一般）
  • インフルエンザウイルス感染の新規バイオマーカーとしてのヒドロキシ脂肪酸の飽和脂肪酸エステル
    Siddabasave Gowda, B. Gowda, D., Gowda, Oono M, Liang C, Chiba H, Hui S-P
    第55回 日本臨床検査医学会北海道支部総会/第31回 日本臨床化学会北海道支部例会. 2021,北海道, 日本. 口頭発表（一般）
  • (肥満の新しいバイオマー カーとしての腸管内容物の ヒドロキシ脂肪酸の短鎖脂 肪酸エステルの発見
    第54回日本臨床検査医 学会・第30回日本臨床 化学会北海道支部例会 (誌上開催)(札幌，日 本)
  • 非標的リピドミクス分析 による心筋梗塞の新しい脂 質バイオマーカー
    Siddabasave gowda, B. Gowda
    第54回日本臨床検査医 学会・第30回日本臨床 化学会北海道支部例会 (誌上開催)(札幌，日 本)

その他活動・業績

特許

  • 特許P2016-79161A:糖スフィンゴシン及びスフィンゴ塩基の製造方法発明者    2016年05月16日
    ボメゴウダシッダバサヴゴウダ

受賞

  • 2022年08月 Hokkaido University 2022年度 次世代研究者共同研究支援制度
     北海道の在来魚シシャモの肥満抑制効果に関する研究 
    受賞者: Siddabasave gowda;Bomme gowda
  • 2022年07月 Hokkaido University 2022年度SOUSEI若手研究者支援プログラム助成金
     脂質代謝に関連する肥満症を食品バイオアクチブで克服する 
    受賞者: Siddabasa gowda;Bomme gowda
  • 2022年03月 2020-2021 Top cited author from the Rapid Communications in Mass spectrometry
  • 2021年11月 Hokkaido University 北海道大学-部局横断型シンポジウム / 助成金-銅賞
     インフルエンザ感染における新規腸管バイオマーカーの役割の検討 
    受賞者: Siddabasave gowda;Bomme Gowda
  • 2021年06月 Hokkaido University 2021年度 次世代研究者共同研究支援制度
     "スフィンゴ脂質代謝に関連する昆布と肥満の関連性を解明" 
    受賞者: Siddabasave gowda;Bomme Gowda
  • 2021年04月 日本学術振興会 若手研究
     肥満に伴うスフィンゴ糖脂質の代謝におけるヒジキの機能的役割を探る 
    受賞者: Siddabasavegowda;Bomme gowda
  • 2020年06月 北海道大学 スタートアップ助成金
  • 2017年12月 TBRF, Japan Postdoctoral fellowship
     TBRF Postdoctoral Fellow (declined) 
    受賞者: ボメゴウダシッダバサヴゴウダ
  • 2016年02月 JASSO, Japan Scholarship
     PhD scholar 
    受賞者: ボメゴウダシッダバサヴゴウダ
  • 2015年07月 ONO Pharmacetical Travel Award
     14th Bioactive lipid conference, Hungary 
    受賞者: ボメゴウダシッダバサヴゴウダ
  • 2015年03月 Hokkaido University Travel Award
     249th ACS meeting, Colorado, USA 
    受賞者: ボメゴウダシッダバサヴゴウダ
  • 2012年10月 Japanese governament IGP-MEXT Scholarship
     Phd Research fellow 
    受賞者: ボメゴウダシッダバサヴゴウダ

共同研究・競争的資金等の研究課題

  • 若手研究
    日本学術振興会：Early Carrier Scientist Award
    研究期間 : 2021年04月 -2025年03月
  • 北海道の在来魚シシャモの肥満抑制効果に関する研究
    北海道大学：2022年度次世代研究者共同研究支援制度。
    研究期間 : 2022年08月 -2023年03月 
    代表者 : Siddabasave gowda, Bomme Gowda
  • 脂質代謝に関連する肥満症を食品バイオアクチブで克服する
    北海道大学：2022年度SOUSEI若手研究者支援プログラム助成金
    研究期間 : 2022年07月 -2023年03月 
    代表者 : Siddabasave gowda, Bomme Gowda
  • インフルエンザ感染における新規腸管バイオマーカーの役割の検討
    北海道大学：The 7th Hokkaido University Cross-Departmental Symposium Research Grant
    研究期間 : 2021年11月 -2022年03月 
    代表者 : Siddabasave gowda, Bomme Gowda
  • "Unrevealing the links between Kombu and obesity associated to sphingolipid metabolism"
    Hokkaido University：FY2021 Support System for the Collaborative Research of Next-Generation Researchers:
    研究期間 : 2021年07月 -2022年02月
  • Start-up grant of 300,000 yen
    Hokkaido University：
    研究期間 : 2019年06月 -2021年05月 
    代表者 : ボメゴウダシッダバサヴゴウダ

教育活動情報

主要な担当授業

  • ワンダーフォーゲル実習Ⅰ
    開講年度 : 2021年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : Please see the English version.
  • 事前・事後演習Ⅰ
    開講年度 : 2021年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : Please see the English version.
  • 代謝分析化学特論
    開講年度 : 2021年
    課程区分 : 修士課程
    開講学部 : 保健科学院
    キーワード : 生活習慣病、メタボリックシンドローム、酸化ストレス、過酸化脂質、LC/MS、質量分析法
  • 代謝分析化学演習
    開講年度 : 2021年
    課程区分 : 修士課程
    開講学部 : 保健科学院
    キーワード : HPLC、質量分析法、LC/MS、NMR、細胞培養、ミトコンドリア、バイオインフォマティクス
  • 食資源と健康
    開講年度 : 2021年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 健康，食，栄養学，環境，人類生態学，人間栄養学，脂質，酸化，糖鎖生物学，蛋白質，腸内環境，機能性食品、ポストハーベスト加工技術
  • 食資源生産論
    開講年度 : 2021年
    課程区分 : 修士課程
    開講学部 : 国際食資源学院
    キーワード : 作物，畜産物，水産物，GAP, バイオテクノロジー，発酵生産，食の安全，HACCP, 機能性食品，健康，エネルギー
  • 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ)
    開講年度 : 2021年
    課程区分 : 学士課程
    開講学部 : 全学教育
    キーワード : 健康、医学、検査、画像診断、がん、血球形態検査、臨床化学、寄生虫、細菌、細胞検査、免疫検査、食品、細菌・共生と感染、心疾患、低比重リポタンパク質、硬さ, 脂質化学
  • 英語演習
    開講年度 : 2021年
    課程区分 : 学士課程
    開講学部 : 全学教育
    キーワード : 科学エッセイ、医学英語、英語論文、英文実験プロトコール、英語によるプレゼンテーション

大学運営

委員歴

  • 2023年04月 - 現在   学生委員会 国際部会 部員
  • 2022年04月 - 2023年03月   健康科学部   親睦会委員
  • 2022年04月 - 2023年03月   入学試験委員会
  • 2022年04月 - 2023年03月   FD委員会
  • 2021年04月 - 2022年03月   親睦会幹事
  • 2021年04月 - 2022年03月   公開講座実行委員会
  • 2020年04月 - 2021年03月   公開講座委員会

メディア報道

  • https://www.global.hokudai.ac.jp/blog/lipids-with-potential-health-benefits-in-herbal-teas/
  • Sphingosine-1-phosphate interactions in the spleen and heart reflect extent of cardiac repair in mice and failing human hearts
    執筆者 : 本人以外
    発行元・放送局 : for details access this link: https://physiology.altmetric.com/details/112226626/news
    　新聞・雑誌

学術貢献活動

  • 国内外との連携活動
    役割 : その他
    種別 : 学術調査
    主催者・責任者 : with Prof. Ganesh Halade, University of South Florida, USA; Dr. Nina Patzke, Heath and Medical University, Germany; Prof. Hye-Won Shin, Kyoto University, Japan; Prof. Hiroshi Kida, Dr. Ohno, International Institute for Zoonosis control, Hokkaido University; Prof. Atsushi Yokota, Faculty of Agriculture, Hokkaido University. 8. Dr. Yaser Hosny Ali Elewa, Faculty of veterinary Medicine, Hokkaido University; Dr. Takayuki Tsukui, Sapporo University of Health Sciences.
  • 各種雑誌の査読者
    役割 : 査読
    種別 : 査読等
    主催者・責任者 : • Food Control (Elsevier) • Phytochemical Analysis (Wiley) • NPJ Science of Food (Springer) • Molecules, Metabolites, Microorganisms, Energies, & IJMS (MDPI)

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