研究者データベース
| 小方 昌平(オガタ シヨウヘイ) |
| 人獣共通感染症国際共同研究所 国際展開推進部門 |
| 助教 |
Last Updated :2023/12/06
研究活動情報
論文
- Shohei Ogata, Rika Umemiya-Shirafuji, Kodai Kusakisako, Keita Kakisaka, Elisha Chatanga, Naoki Hayashi, Yurie Taya, Yuma Ohari, Gita Sadaula Pandey, Abdelbaset Eweda Abdelbaset, Yongjin Qiu, Keita Matsuno, Nariaki Nonaka, Ryo NakaoScientific Reports 13 1 2023年08月15日 [査読有り]
Abstract Many arthropods harbour bacterial symbionts, which are maintained by vertical and/or horizontal transmission. Spiroplasma is one of the most well-known symbionts of ticks and other arthropods. It is still unclear how Spiroplasma infections have spread in tick populations despite its high prevalence in some tick species. In this study, Ixodes ovatus, which has been reported to harbour Spiroplasma ixodetis at high frequencies, was examined for its vertical transmission potential under experimental conditions. Next, two isolates of tick-derived Spiroplasma, S. ixodetis and Spiroplasma mirum, were experimentally inoculated into Spiroplasma-free Haemaphysalis longicornis colonies and the presence of Spiroplasma in their eggs and larvae was tested. Our experimental data confirmed that S. ixodetis was transmitted to eggs and larvae in a vertical manner in the original host I. ovatus. In the second experiment, there was no significant difference in engorged weight, egg weight, and hatching rate between Spiroplasma-inoculated and control H. longicornis groups. This suggested that Spiroplasma infection does not affect tick reproduction. Spiroplasma DNA was only detected in the eggs and larvae derived from some individuals of S. ixodetis-inoculated groups. This has demonstrated the potential of horizontal transmission between different tick species. These findings may help understand the transmission dynamics of Spiroplasma in nature and its adaptation mechanism to host arthropod species. - Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, May June Thu, Keita Kakisaka, Elisha Chatanga, Shohei Ogata, Naoki Hayashi, Yurie Taya, Yuma Ohari, Doaa Naguib, Yongjin Qiu, Keita Matsuno, Saw Bawm, Lat Lat Htun, Stephen C. Barker, Ken Katakura, Kimihito Ito, Nariaki Nonaka, Ryo NakaoEvolutionary Applications. 15 7 1062 - 1078 2022年06月 [査読有り]
- Elisha Chatanga, Emmanuel Maganga, Wessam Mohamed Ahmed Mohamed, Shohei Ogata, Gita Sadaula Pandey, Abdelbaset, Eweda Abdelbaset, Kyoko Hayashida, Chihiro Sugimoto, Ken Katakura, Nariaki Nonaka, Ryo NakaoActa Tropica 231 106413 - 106413 2022年03月 [査読有り]
Malawi has an estimated cattle population of 1,884,803 heads, the indigenous Malawi zebu breed accounts for 91.2%, while the exotic and crossbred accounts for the remaining 8.8%. Although ticks and tick-borne diseases are widespread in Malawi, no molecular study has been conducted to investigate the tick-borne Anaplasmataceae and piroplasms infecting cattle. To provide an insight into the current status of tick-borne pathogens (TBPs) of cattle, a molecular survey was conducted in the central and southern regions of Malawi. A total of 191 cattle of which 132 were Malawi zebu, 44 were Holstein Friesian and 15 were Holstein-Friesian/ Malawi zebu crosses were screened for Anaplasmataceae and piroplasms using the heat shock protein groEL gene and 18S rDNA, respectively. A new 18S rDNA multiplex PCR assay was designed for Babesia and Theileria species identification without sequencing. Overall, 92.3% (n = 177) of the examined animals were infected with at least one TBP. Anaplasmataceae-positive rate was 57.6% (n = 110) while for piroplasms it was 80.1% (n = 153). The detected Anaplasmataceae were Anaplasma bovis 2.6% (n = 5), Anaplasma marginale 24.6% (n = 47), Anaplasma platys-like 13.6% (n = 26), uncharacterized Anaplasma sp. 14.1% (n = 27), and uncharacterized Ehrlichia sp. 16.2% (n = 31). The detected piroplasms were Babesia bigemina 2.6% (n = 5), Theileria mutans 73.8% (n = 141), Theileria parva 33.0% (n = 63), Theileria taurotragi 12.6% (n = 24), and Theileria velifera 53.4% (n = 102). Mixed infection rate was found in 79.6% (n = 152) of the samples analyzed. This study has shown a high burden of TBPs among cattle in Malawi which highlights the need to conceive new methods to control ticks and TBPs in order to improve animal health and productivity. The newly developed multiplex PCR assay would be a useful tool especially in resource limited settings where sequencing is not available and when mixed infections are expected. - Shohei Ogata, Juan Antonio Cristian Pereira, Loza Vega Ariel Jhonny, Herbas Perez Gladys Carolina, Keita Matsuno, Yasuko Orba, Hirofumi Sawa, Fumihiko Kawamori, Nariaki Nonaka, Ryo NakaoVeterinary Sciences 8 9 188 - 188 2021年09月07日 [査読有り][通常論文]
Latin American countries produce more than a quarter of the world’s beef and are a major global supplier of livestock protein. Tick-borne diseases (TBDs) are a major constraint to the livestock industry worldwide, including in Latin America. The aim of this study was to detect and characterise tick-borne pathogens in cattle from Santa Cruz, Bolivia, where no detailed epidemiological data are available. Blood samples were collected from 104 cattle. Apicomplexan parasites were detected by nested PCR amplification of the 18S ribosomal RNA gene (rDNA), and Anaplasmataceae was screened by the PCR amplification of 16S rDNA, followed by characterisation based on the heat shock protein and citrate synthase gene sequences. Babesia infection was observed in nine cattle (one Babesia bovis and eight Babesia bigemina), while Anaplasmataceae infection was detected in thirty-two cattle. A sequencing analysis confirmed the presence of Anaplasma marginale and Anaplasma platys-like. These results provide the first molecular evidence for the four above-mentioned tick-borne pathogens in cattle in Bolivia. This information improves our understanding of the epidemiology of TBDs and will help in formulating appropriate and improved pathogen control strategies. - Yurie Taya, Gohta Kinoshita, Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, Shohei Ogata, Elisha Chatanga, Yuma Ohari, Kodai Kusakisako, Keita Matsuno, Nariaki Nonaka, Ryo NakaoMicroorganisms 9 5 2021年05月13日 [査読有り]
- Shohei Ogata, Wessam Mohamed Ahmed Mohamed, Kodai Kusakisako, May June Thu, Yongjin Qiu, Mohamed Abdallah Mohamed Moustafa, Keita Matsuno, Ken Katakura, Nariaki Nonaka, Ryo NakaoMicroorganisms 9 2 2021年02月08日 [査読有り][通常論文]
- Ryo Nakao, Kohei Shinjo, Tomoki Sakiyama, Shohei Ogata, Kodai Kusakisako, Gohta Kinoshitaa, Doaa Naguiba, Elisha Chatanga, Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, Keita Matsuno, Takuya Itoi, Nariaki Nonaka, Mariko Sashika, Toshio Tsubota, Michito ShimozuruParasitology International 80 2021年02月 [査読有り]
The tick Amblyomma testudinarium Koch, 1844 (Acari: Ixodidae) is known as a vector of several pathogens such as Rickettsia tamurae and severe fever with thrombocytopenia syndrome (SFTS) virus. This tick species is present in many Asian countries, including Japan, where its distribution is limited to the warm areas of Kanto region and the southwestern region. The present study reports the recovery of a partially engorged A. testudinarium from a wild brown bear captured in Shari town, Hokkaido. In addition to morphological identification, the specimen was genetically characterized by the complete mitochondrial genome sequencing. The results showed that the length of the obtained mitogenome is 14,835 bp that encodes 13 protein-coding, two ribosomal RNA (rRNA) (12S and 16S), and 22 transfer RNA genes with two non-coding control regions. The phylogenetic analysis indicated that our sample clustered with A. testudinarium from Nara, Japan, but separated from A. testudinarium from China. Although the introduction of the tick through livestock transportation cannot be ruled out, the detection of A. testudinarium in Hokkaido prefecture, which is separated from the main island where A. testudinarium is present in the south, may suggest the introduction by migratory birds. This study provides important insights on the distribution and host range of A. testudinarium. This will be useful for the future taxonomic analysis of ticks based on the complete mitogenome sequencing. To our knowledge, this is the northernmost detection point of the tropical tick A. testudinarium. - May June Thu, Yongjin Qiu, Junya Yamagishi, Kodai Kusakisako, Shohei Ogata, Mohamed Abdallah Mohamed Moustafa, Norikazu Isoda, Chihiro Sugimoto, Ken Katakura, Nariaki Nonaka, Ryo NakaoAmerican Society for Microbiology 8 37 2019年09月12日 [査読有り]
This is the first report of the complete genome sequence ofRickettsia asiatica strain Maytaro1284, isolated from anIxodes ovatus tick in Japan. The genome contains a 1,344,324-bp circular chromosome and one plasmid of 74,761 bp. There was no outer membrane protein A (ompA ) gene encoded in the genome. - Yasuko Sekita, Keiji Murakami, Hiromichi Yumoto, Takashi Amoh, Natsumi Fujiwara, Shohei Ogata, Takashi Matsuo, Yoichiro Miyake, Yoshiki KashiwadaBioMed Research International 2016 2016年06月20日 [査読有り]
講演・口頭発表等
- Zoonosis surveillance in Zambia and academic contribution to foster zoonosis experts under the One Health concept. [通常講演]3rd International Conference on Public Health in Africa (CPHIA 2023) 2023年11月 シンポジウム・ワークショップパネル（公募）
- マダニ × ファッション - 従来のマダニ感染症の啓蒙活動に対する問題へのファッションを取り入れた試み - [通常講演]小方昌平, 西晃平超異分野学会 東京大会2023 2023年03月 ポスター発表
- ボリビア多民族国サンタクルス県の牛におけるBabesiaおよびAnaplasmaの感染状況 [通常講演]小方昌平、Juan Antonio Pereira、松野啓太、大場靖子、澤洋文、川森文彦、野中成晃、中尾亮第164回日本獣医学会学術集会 2021年09月 口頭発表（一般）
- 人への病原性が示唆されるマダニ共生細菌の検出および⼆酸化炭素誘引法による⽇本産マダニ採集への挑戦 [通常講演]小方昌平、Wessam Mohamed Ahmed Mohamed、草木迫浩大、May June Thu、邱永晋、Mohamed Abdallah Mohamed Moustafa、松野啓太、片倉賢、野中成晃、中尾亮第２８回ダニと疾患のインターフェイスに関するセミナー 2021年09月
- Molecular analysis of Babesia and Anaplasma in cattle in Bolivia. [通常講演]Shohei Ogata, Loza Vega Ariel Jhonny, Juan Antonio C. Pereira, Keita Matsuno, Nariaki Nonaka, Ryo NakaoThe 8th Sapporo Summer Symposium for One Health 2020 2020年09月 口頭発表（一般）
- The diversity of Spiroplasma sp. in ticks.Shohei Ogata, Ryo Nakao, Nariaki NonakaThe 7th Sapporo Summer Symposium for One Health 2019 2019年09月 ポスター発表
- マダニが保有するスピロプラズマ属細菌の多様性 [通常講演]小方昌平、中尾亮、野中成晃、片倉賢第162回日本獣医学会学術集会 2019年09月 口頭発表（一般）
- Analysis of symbionts and parasites for vector-borne disease control. [通常講演]Shohei Ogata, Yuki Eshita, Ryo NakaoThe 6th Sapporo Summer Symposium for One Health 2018 2018年09月 ポスター発表
その他活動・業績
- 北海道斜里町においてエゾヒグマより採集されたタカサゴキララマダニの一例下鶴 倫人, 新庄 康平, 崎山 智樹, 小方 昌平, 草木迫 浩大, 木下 豪太, Mohamed Wessam Mohamed Ahmed, Moustafa Mohamed Abdallah Mohamed, 松野 啓太, 伊東 拓也, 野中 成晃, 佐鹿 万里子, 坪田 敏男, 中尾 亮 日本獣医学会学術集会講演要旨集 163回 327 -327 2020年10月