研究者データベース

落合 正則(オチアイ マサノリ)
低温科学研究所 生物環境部門
准教授

基本情報

所属

  • 低温科学研究所 生物環境部門

職名

  • 准教授

学位

  • 理学博士(北海道大学)

ホームページURL

科研費研究者番号

  • 10241382

J-Global ID

研究キーワード

  • 生化学   昆虫生理学   分子生物学   Insect physiology   Molecular Biology   Biochemistry   

研究分野

  • 環境・農学 / 植物保護科学
  • 環境・農学 / 植物保護科学
  • ライフサイエンス / 機能生物化学
  • ライフサイエンス / 動物生理化学、生理学、行動学

職歴

  • 2010年 - 北海道大学 低温科学研究所 准教授

所属学協会

  • 日本応用動物昆虫学会   日本生化学会   日本動物学会   

研究活動情報

論文

  • Masasuke Ryuda, Miku Tabuchi, Hitoshi Matsumoto, Takashi Matsumura, Masanori Ochiai, Yoichi Hayakawa
    Archives of Insect Biochemistry and Physiology 97 3 e21440  2018年03月01日 [査読有り][通常論文]
     
    Recovery from weight loss after stress is important for all organisms, although the recovery mechanisms are not fully understood. We are working to clarify these mechanisms. Here, we recorded enhanced feeding activity of Drosophila melanogaster larvae from 2 to 4 h after heat stress at 35°C for 1 h. During the post-stress period, expression levels of sweet taste gustatory receptor genes (Grs), Gr5a, Gr43a, Gr64a, and Gr64f, were elevated, whereas bitter taste Grs, Gr66a, and Gr33a, were decreased in expression and expression of a non-typical taste receptor Gr, Gr68a, was unchanged. Similar upregulation of Gr5a and downregulation of Gr66a was recorded after cold stress at 4°C. Expression levels of tropomyosin and ATP synthase ß subunit were significantly increased in larval mouth parts around 3 to 5 h after the heat stress. We infer that up-regulation of post-stress larval feeding activity, and weight recovery, is mediated by increasing capacity for mouth part muscular movements and changes in taste sensing physiology. We propose that Drosophila larvae, and likely insects generally, express an efficient mechanism to recover from weight loss during post-stress periods.
  • Cytokine signaling through Drosophila Mthl10 ties lifespan to environmental stress
    Sung E. J, Ryuda M, Matsumoto, H, Uryu O, Ochiai, M, Cook M, Yi N, Y. Wang H, Putney Jr. J, Bird G, Shears S. B, Hayakawa, Y
    Proc. Natl. Acad. Sci. USA 114 13786 - 13791 2017年 [査読有り][通常論文]
  • Yoshiharu Toyama, Masahiro Shimizu, Masanori Ochiai, Toshiaki Dobashi
    Journal of Biorheology 31 1 12 - 15 2017年 [査読有り][通常論文]
     
    Fibrinogen is converted to fibrin monomer and forms a three-dimensional gel network in a step wise manner by the enzymatic action of thrombin (thrombin-induced fibrin gelation). On the other hand, when a fibrinogen solution is incubated at low temperatures (e.g. below 4°C), it is converted to a gel without thrombin. The fibrinogen gel induced by lowering temperature is called “cryogel” and is closely related to some diseases. However, the mechanism of fibrinogen cryogelation has not been understood yet. In this study, the effects of urea and NaCl on fibrinogen cryogelation and thrombin-induced fibrin gelation have been studied turbidimetrically. The addition of urea retarded both gelation, and the extent of retardation increased with increasing the concentration of urea. The effects of urea on fibrinogen cryogelation were much larger than those on thrombin-induced fibrin gelation, and fibrinogen cryogelation was completely inhibited by the addition of 150 mM urea. The addition of NaCl retarded fibrinogen cryogelation with increasing the concentration of NaCl. By contrast, the effects of NaCl on thrombin-induced fibrin gelation were only limited. Based on the experimental results, the contributions of hydrogen and ionic bonds to each gelation are discussed.
  • Group-housed females promote asexual ootheca production in American cockroaches
    Katoh K, Iwasaki M, Hosono S, Yoritsune A, Ochiai M, Mizunami M, Nishino H
    Zoological Letters 3 3  2017年 [査読有り][通常論文]
  • Kakeru Yokoi, Yuuki Hayakawa, Daiki Kato, Chieka Minakuchi, Toshiharu Tanaka, Masanori Ochiai, Katsumi Kamiya, Ken Miura
    JOURNAL OF INVERTEBRATE PATHOLOGY 132 190 - 200 2015年11月 [査読有り][通常論文]
     
    In this study, we characterized prophenoloxidase (proPO, (PPO)) genes of Tribolium castaneum and examined their involvement in antimicrobial host defense. Amino acid sequence comparison with well-characterized PPO proteins from other insect species suggested that T. castaneum PPO genes encoded functional proenzymes, with crucial sequence motifs being conserved. Developmental kinetics of the mRNA of two PPO genes, PPO1 and PPO2 in the pupal stage were different to each other. The PPO1 mRNA levels consistently decreased during pupal development while that of PPO2 peaked at mid-pupal stage. The two mRNAs also exhibited distinct responses upon immune challenges with heat-killed model microbes. The PPO1 mRNA stayed nearly unchanged by 6 h post challenge, and was somewhat elevated at 24 h. In contrast, the PPO2 mRNA significantly decreased at 3, 6 and 24 h post challenge. These trends exhibited by respective PPO genes were consistent irrespective of the microbial species used as elicitors. Finally, we investigated the involvement of T. castaneum PPO genes in antimicrobial host defense by utilizing RNA interference-mediated gene silencing. Survival assays demonstrated that double knockdown of PPO genes, which was accompanied by weakened hemolymph PO activities, significantly impaired the host defense against Bacillus subtilis. By contrast, the knockdown did not influence the induction of any of the T. castaneum antimicrobial peptide genes that were studied here, except for one belonging to the gene group that shows very weak or negligible microbial induction. PPO knockdown as well weakened host defense against Beauveria bassiana moderately but significantly depending on the combination of infection methods and targeted genes. Our results indicated that the PPO genes represented constituents of both antibacterial and antifungal host defense of T. castaneum. (C) 2015 Elsevier Inc. All rights reserved.
  • Takeshi Kawano, Masasuke Ryuda, Hitoshi Matsumoto, Masanori Ochiai, Yasunori Oda, Teiichi Tanimura, Gyorge Csikos, Megumi Moriya, Yoichi Hayakawa
    SCIENTIFIC REPORTS 5 17195  2015年11月 [査読有り][通常論文]
     
    Desiccate (Desi), initially discovered as a gene expressing in the epidermis of Drosophila larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that Desi is expressed in two different types of non-neuronal cells of the labellum, the epidermis and thecogen accessory cells. Labellar Desi expression was significantly elevated under arid conditions, accompanied by an increase in water ingestion by adults. Desi overexpression also promoted water ingestion. In contrast, a knockdown of Desi expression reduced feeding as well as water ingestion due to a drastic decrease in the gustatory sensillar sensitivity for all tested tastants. These results indicate that Desi helps protect insects from desiccation damage by not only preventing dehydration through the integument but also accelerating water ingestion via elevated taste sensitivities of the sensilla.
  • Xueyun Hu, Satoru Makita, Silvia Schelbert, Shinsuke Sano, Masanori Ochiai, Tohru Tsuchiya, Shigeaki F. Hasegawa, Stefan Hoertensteiner, Ayumi Tanaka, Ryouichi Tanaka
    PLANT PHYSIOLOGY 167 3 660 - + 2015年03月 [査読有り][通常論文]
     
    Chlorophyllase (CLH) is a common plant enzyme that catalyzes the hydrolysis of chlorophyll to form chlorophyllide, a more hydrophilic derivative. For more than a century, the biological role of CLH has been controversial, although this enzyme has been often considered to catalyze chlorophyll catabolism during stress-induced chlorophyll breakdown. In this study, we found that the absence of CLH does not affect chlorophyll breakdown in intact leaf tissue in the absence or the presence of methyljasmonate, which is known to enhance stress-induced chlorophyll breakdown. Fractionation of cellular membranes shows that Arabidopsis (Arabidopsis thaliana) CLH is located in the endoplasmic reticulum and the tonoplast of intact plant cells. These results indicate that CLH is not involved in endogenous chlorophyll catabolism. Instead, we found that CLH promotes chlorophyllide formation upon disruption of leaf cells, or when it is artificially mistargeted to the chloroplast. These results indicate that CLH is responsible for chlorophyllide formation after the collapse of cells, which led us to hypothesize that chlorophyllide formation might be a process of defense against chewing herbivores. We found that Arabidopsis leaves with genetically enhanced CLH activity exhibit toxicity when fed to Spodoptera litura larvae, an insect herbivore. In addition, purified chlorophyllide partially suppresses the growth of the larvae. Taken together, these results support the presence of a unique binary defense system against insect herbivores involving chlorophyll and CLH. Potential mechanisms of chlorophyllide action for defense are discussed.
  • Yoshiaki Kato, Tatsuhiro Yoshida, Ken Miura, Toshiharu Tanaka, Yutaka Nakamatsu, Masanori Ochiai
    ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 86 4 220 - 239 2014年08月 [査読有り][通常論文]
     
    Lepidopteran larvae show a cellular response to invading foreign substances that are larger than hemocytes, for example, parasitoid eggs or larvae. This response is called hemocyte encapsulation and is often accompanied by phenoloxidase (PO)-catalyzed melanization. In the present study, we artificially transplanted endoparasitoid larvae and small glass fragments into the hemocoel of the common armyworm, Mythimna separata. We observed that the host larva showed a cellular response and that, 2-4 h after transplantation, melanin formation was spatially confined to the surface of the encapsulated substances. We further noted that specific morphological hemocytes surrounded by melanin formation became attached to the surface of the foreign substances. We designated these hemocytes hyperspread cells (HSCs) on the basis of their specific characteristics and circumferential spread. We confirmed the occurrence of prophenoloxidase (PPO)/phenoloxidase (PO) on the periphery of the HSCs and in the substance secreted around the HSCs by using anti-PPO antibody. We were unable to detect PPO-mRNA in HSCs by using in situ hybridization, although we showed that oenocytoids contained PPO-mRNA and PPO protein. We used light microscopy and scanning electron microscopy to discriminate five main types of circulating M. separata hemocytes. We observed that HSCs differed from plasmatocytes, but spread out well. Further, during the encapsulation process, HSCs appeared to provide a localized melanization spot on the surface of foreign invaders. (C) 2014 Wiley Periodicals, Inc.
  • Shunsuke Furihata, Kohjiro Tanaka, Masasuke Ryuda, Masanori Ochiai, Hitoshi Matsumoto, Gyorge Csikos, Yoichi Hayakawa
    JOURNAL OF INVERTEBRATE PATHOLOGY 115 26 - 32 2014年01月 [査読有り][通常論文]
     
    Polydnaviruses (PDVs) are unique symbiotic viruses associated with parasitoid wasps: PDV particles are injected into lepidopteran hosts along with the wasp eggs and express genes that interfere with aspects of host physiology such as immune defenses and development. Recent comparative genomic studies of PDVs have significantly improved our understanding of their origin as well as the genome organization. However, the structural features of functional PDV particles remain ambiguous. To clear up the structure of Cotesia kariyai PDV (CkPDV) particles, we focused on immunoevasive protein (IEP), which is a mediator of immunoevasion by the wasp from the encapsulation reaction of the host insect's hemocytes, because it has been demonstrated to be present on the surface of the virus particle. We discovered that IEP tends to polymerize and constitutes a previously unidentified thin surface layer covering CkPDV particles. This outermost surface layer looked fragile and was easily removed from CkPVD particles by mechanical stressors such as shaking, which prevented CkPDV from expressing the encoded genes in the host target tissues such as fat body or hemocytes. Furthermore, we detected IEP homologue gene expression in the wasp's venom reservoirs, implying IEP has another unknown biological function in the wasp or parasitized hosts. Taken together, the present results demonstrated that female C kariyai wasps produce the fragile thin layer partly composed of IEP to cover the outer surfaces of CkPDV particles; otherwise, they cannot function as infectious agents in the wasp's host. The fact that IEP family proteins are expressed in both venom reservoirs and oviducts suggests an intimate relationship between both tissues in the development of the parasitism strategy of the wasp. (C) 2013 Elsevier Inc. All rights reserved.
  • Horiuchi, M, Takahasi, K, Kobashigawa, Y, Ochiai, M, Inagaki, F
    PEDS. 25 8 405 - 413 2012年08月 [査読有り][通常論文]
     
    Silkworm -1,3-glucan recognition protein (GRP) tightly and specifically associates with -1,3-glucan. We report here an affinity purification system named the oGRP system', which uses the association between the -1,3-glucan recognition domain of GRP (GRP-tag), as an affinity tag, and curdlan beads. Curdlan is a water-insoluble -1,3-glucan reagent, the low cost of which (about 100 JPY/g) allows the economical preparation of beads. Curdlan beads can be readily prepared by solubilization in an alkaline solution, followed by neutralization, sonication and centrifugation. We applied the GRP system to preparation of several proteins and revealed that the expression levels of the GRP-tagged proteins in soluble fractions were two or three times higher than those of the glutathione S-transferase (GST)-tagged proteins. The purity of the GRP-tagged proteins on the curdlan beads was comparable to that of the GST-tagged proteins on glutathione beads. The chemical stability of the GRP system was more robust than conventional affinity systems under various conditions, including low pH (46). Biochemical and structural analyses revealed that proteins produced using the GRP system were structurally and functionally active. Thus, the GRP system is suitable for both the large- and small-scale preparation of recombinant proteins for functional and structural analyses.
  • Koichiro Yamaguchi, Hitoshi Matsumoto, Masanori Ochiai, Seiji Tsuzuki, Yoichi Hayakawa
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 42 3 183 - 192 2012年03月 [査読有り][通常論文]
     
    Growth rates of immature animals are governed by their feeding activities. A reduction in feeding sometimes causes serious growth retardation in insects; a typical case is often seen in host insects parasitized by a solitary endoparasitoid wasp. However, understanding of the mechanisms underlying the physiological repression of parasitized insects is fragmentary. Here we analyzed brain gene expression of the host common cutworm, Spodoptera litura, parasitized by a solitary endoparasitoid, Microplitis manilae, and identified a novel gene whose expression was significantly enhanced by parasitization. The gene encoded a pre-pro-peptide of a cytokine-like molecule and its expression was observed mainly in nervous tissues, hemocytes, and integuments. The 25 amino acid cytokine-like peptide encoded by the C-terminus of this gene was demonstrated to exist in the hemolymph of S. litura larvae and to change hemocytes from non-adhesive to adhesive in vitro. Further, injection of the active peptide reduced feeding activities of test larvae and consequently delayed their growth. The enhanced gene expression was also observed in larvae under severe stress conditions: abdominal ligature, proleg cutting, mechanical vibration, low temperature, and heat shock at 45 C. Elevated gene expression was maintained only in seriously growth-retarded larvae but not in recovered larvae at 24 h or 48 h after heat treatment. Thus, it is reasonable to conclude that stress-induced elevation of the peptide gene expression highly correlates with reduced feeding activities and growth retardation of the host larvae parasitized by M. manilae. Based on the conclusion, we named this peptide stress-responsive peptide (SRP). (C) 2011 Elsevier Ltd. All rights reserved.
  • Seiji Tsuzuki, Masanori Ochiai, Hitoshi Matsumoto, Shoichiro Kurata, Atsushi Ohnishi, Yoichi Hayakawa
    SCIENTIFIC REPORTS 2 210  2012年01月 [査読有り][通常論文]
     
    Antimicrobial peptides (AMPs), major innate immune effectors, are induced to protect hosts against invading microorganisms. AMPs are also induced under non-infectious stress; however, the signaling pathways of non-infectious stress-induced AMP expression are yet unclear. We demonstrated that growth-blocking peptide (GBP) is a potent cytokine that regulates stressor-induced AMP expression in insects. GBP overexpression in Drosophila elevated expression of AMPs. GBP-induced AMP expression did not require Toll and immune deficiency (Imd) pathway-related genes, but imd and basket were essential, indicating that GBP signaling in Drosophila did not use the orthodox Toll or Imd pathway but used the JNK pathway after association with the adaptor protein Imd. The enhancement of AMP expression by non-infectious physical or environmental stressors was apparent in controls but not in GBP-knockdown larvae. These results indicate that the Drosophila GBP signaling pathway mediates acute innate immune reactions under various stresses, regardless of whether they are infectious or non-infectious.
  • Toyama,Y, Miyamoto, K, Kubota, K, Wakamatsu, K, Nameki, N, Saheki, T, Ochiai, M
    Trans. MRS-J. 36 393 - 396 2011年 [査読有り][通常論文]
  • Yatagai,Y, Kubota, K, Toyama,Y, Nameki, N, Ochiai, M
    Trans. MRS-J. 36 371 - 374 2011年 [査読有り][通常論文]
  • Kubota, K, Yatagai,Y, Watanabe, N, Fukudal, T, Toyama,Y, Nameki, N, Ochiai, M
    Trans. MRS-J. 36 375 - 378 2011年 [査読有り][通常論文]
  • Yasunori Oda, Hitoshi Matsumoto, Maiko Kurakake, Masanori Ochiai, Atsushi Ohnishi, Yoichi Hayakawa
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107 36 15862 - 15867 2010年09月 [査読有り][通常論文]
     
    Growth-blocking peptide (GBP) is an insect cytokine that stimulates a class of immune cells called plasmatocytes to adhere to one another and to foreign surfaces. Although extensive structure-activity studies have been performed on the GBP and its mutants in Lepidoptera Pseudaletia separata, the signaling pathway of GBP-dependent activation of plasmatocytes remains unknown. We identified an adaptor protein (P77) with a molecular mass of 77 kDa containing SH2/SH3 domain binding motifs and an immunoreceptor tyrosine-based activation motif (ITAM)-like domain in the cytoplasmic region of the C terminus. Although P77 showed no capacity for direct binding with GBP, its cytoplasmic tyrosine residues were specifically phosphorylated within seconds after GBP was added to a plasmatocyte suspension. Tyrosine phosphorylation of P77 also was observed when hemocytes were incubated with Enterobactor cloacae or Micrococcus luteus, but this phosphorylation was found to be induced by GBP released from hemocytes stimulated by the pathogens. Tyrosine phosphorylation of the integrin beta subunit also was detected in plasmatocytes stimulated by GBP. Double-stranded RNAs targeting P77 not only decreased GBP-dependent tyrosine phosphorylation of the integrin beta subunit, but also abolished GBP-induced spreading of plasmatocytes on foreign surfaces. P77 RNAi larvae also showed significantly higher mortality than control larvae after infection with Serratia marcescens, indicating that P77 is essential for GBP to mediate a normal innate cellular immunity in insects. These results demonstrate that GBP signaling in plasmatocytes requires the adaptor protein P77, and that active P77-assisted tyrosine phosphorylation of integrins is critical for the activation of plasmatocytes.
  • Jon Nield, Megumi Moriya, Masanori Ochiai
    MOLECULAR REPRODUCTION AND DEVELOPMENT 77 9 738 - 738 2010年09月 [査読有り][通常論文]
  • Takahasi K, Ochiai M, Horiuchi M, Kumeta H, Ogura K, Ashida M, Inagaki F
    Proc Natl Acad Sci USA 106 28 11679 - 11684 2009年 [査読有り][通常論文]
  • Kenji Kuboa, Yuka Masuda, Yoshiharu Toyama, Nobukazu Nameki, Nobuo Okumura, Masanori Ochiai
    GELS: STRUCTURES, PROPERTIES, AND FUNCTIONS 136 187 - + 2009年 [査読有り][通常論文]
     
    In order to examine the role of alpha C domains, especially the terminal region of it, of fibrinogen A alpha chain in the fibrin gel formation, we prepared a recombinant fibrinogen, A alpha 570 fibrinogen. A alpha 570 fibrinogen is the fibrinogen that is truncated at A alpha 570 and lacks 40 amino acids at the terminus of the alpha C domain. We examined the thrombin-catalyzed polymerization by transmission spectroscopy and confocal laser scanning microscopy (CLSM). We found that A alpha 570 fibrinogen exhibited a significantly delayed aggregation showing the importance of the terminal region of the alpha C domain in the polymerization process. Contrary to the fact that the addition of glucose to the mixture of fibrinogen and thrombin results in a substantial delay of the lateral aggregation of protofibrils for the native fibrinogen, delaying effect due to the addition of glucose disappeared thoroughly in the case of A alpha 570 fibrinogen. Turbidity measurements dependent upon the wavelength in the time course of gelation showed that mass per unit fiber length of A alpha 570 fibrinogen decreased significantly compared to the native fibrinogen, and the lateral aggregation of protofibrils was hindered significantly. Those results are consistent with the CLSM measurements that the bundles of protofibrils of A alpha 570 fibrinogen are thinner and denser with more branching than those of the native one. It was confirmed that C-terminal region of the alpha C domain plays an important role in the lateral aggregation and glucose interferes the interacting process between the alpha C domains.
  • Heat Shock Proteins in Rat Testis as calmodulin-binding proteins
    Moriya, M, Ochiai, M
    Trend in Developmental Biology 3 1 - 10 2008年 [査読有り][通常論文]
  • Stereoisomer Effect of Disaccharides on the Interaction with Fibrinogen.
    Masuda, Y, Kogure, H, Ishii, T, Toyama, Y, Wakamatsu, K, Kubota, K, Ochiai, M
    Trans. MRS-J. 31 751 - 754 2006年 [査読有り][通常論文]
  • Effect of Saccharides on the Fibrinogen Gelation Induced by Low Temperature.
    Toyama, Y, Kawashima, Y, Masuda, Y, Kogure, H, Kubota, K, Ochiai, M
    Trans. MRS-J. 31 747 - 750 2006年 [査読有り][通常論文]
  • Effect of NDSB on the Fibrinogen Gelation by Thrombin.
    Kogure, H, Masuda, Y, Ishii, T, Wakamatsu, K, Kubota, K, Ochiai, M
    Trans. MRS-J. 31 787 - 790 2006年 [査読有り][通常論文]
  • Jang IH, Chosa N, Kim SH, Nam HJ, Lemaitre B, Ochiai M, Kambris Z, Brun S, Hashimoto C, Ashida M, Brey PT, Lee WJ
    Dev. Cell 10 1 45 - 55 2006年 [査読有り][通常論文]
  • Kaya, M, Toyama, Y, Kubota, K, Nodasaka, Y, Ochiai M, Nomizu, M, Nishi, N
    Int. J. Biol. Macromol 35 1-2 39 - 46 2005年03月 [査読有り][通常論文]
     
    Using various types of DNAs prepared from plasmid DNA, complete double-stranded DNA (ds.DNA) with linear and cyclic forms and double-stranded DNA coexisting with single-stranded DNA (ss.DNA), the structure and fibrillogenesis of the collagen-DNA complex were investigated by means of turbidity, transmission I electron microscopy, and confocal laser-scanning microscopy. The rate of fibrillogenesis of the collagen-DNA complex significantly depends on the DNA structure. The structure of the fibrils formed in the complexes showed a marked difference between the ds.DNA and ss.DNA complexes with collagen. Spatial distribution of the DNA and collagen in the complexes suggests that the characteristic collagen-DNA interaction depends on the DNA forms. (c) 2004 Elsevier B.V. All rights reserved.
  • Jo, S, Ochiai M, Furuta, K, Yagi, K
    J. Japan. Soc. Hort. Sci. 74 3 234 - 241 2005年 [査読有り][通常論文]
  • M Moriya, M Ochiai, HJ Yuasa, N Suzuki, M Yazawa
    MOLECULAR REPRODUCTION AND DEVELOPMENT 69 3 316 - 324 2004年11月 [査読有り][通常論文]
     
    Ca2+-calmodulin (CaM)-binding proteins in rat testes were characterized by assays for CaM-binding activity using the CaM-overlay method on transblots of electrophoresed gels and purification by gel-filtration, ion exchange, and adsorption chromatographies. A major CaM-binding protein complex (CaMBP) was identified and found to be comprised of three proteins with molecular masses 110, 100, and 70 kDa. Amino acid sequence analyses of lysylendo-peptidase digests from these proteins indicated that all of the constituents of CaMBP are very similar to the members of the heat-shock protein family, i.e., the 110-kDa protein is similar to the APG-2/94 kDa rat ischemia-responsive protein, the 100-kDa protein is similar to the rat counterpart of the mouse APG-1/94 kDa osmotic stress protein, and the 70-kDa protein is similar to the rat testis-specific major heat-shock protein (HSP70). Immunohistochemistry using anti-CaMBP and anti-CaM antibodies demonstrated that CaMBP was co-localized with CaM in the cytoplasm of pachytene spermatocytes and nuclei of round spermatids. In addition, CaMBP, but not CaM, was localized at a high level in the residual bodies of elongated spermatids. The possible relevance of CaMBP to regulation of cell cycle progression and spermatogenesis is discussed in this paper. (C) 2004 Wiley-Liss, Inc.
  • Effects of Mono-, Oligo- and Polysaccharides on Fibrin Gelation.
    Masuda, Y, Toyama, Y, Kogure, H, Kubota, K, Ochiai, M
    Trans. MRS-J. 29 3331 - 3335 2004年 [査読有り][通常論文]
  • Effect of saccharides on the fibrinogen-Fibrin conversion by thrombin.
    Kogure, H, Kitazawa, M, Toyama, Y, Kubota, K, Ochiai, M
    Trans. MRS-J. 28 949 - 952 2003年 [査読有り][通常論文]
  • Effect of DNA structure on collagen fibrillogenesis.
    Kaya, M, Toyama, Y, Kubota, K, Nodasaka, Y, Ochiai, M, Nomizu, M, Nishi, N
    Peptide Sci. 379 - 382 2003年 [査読有り][通常論文]
  • K Kadota, E Satoh, M Ochiai, N Inoue, N Tsuji, Igarashi, I, H Nagasawa, T Mikami, FG Claveria, K Fujisaki
    PARASITOLOGY RESEARCH 88 8 781 - 784 2002年08月 [査読有り][通常論文]
     
    Phenol oxidase (PO, EC 1.10.3.1) activity was detected in the hemolymph of the fourth instar nymphs of the argasid tick, Ornithodoros moubata, with peak levels corresponding to the days before the majority of the nymphs had molted, suggestive of a protective role of PO during the ecdysial phase. Higher PO activity was detected in plasma relative to the hemolymph and was negligible in hemocytes. The concentration of the hemolymph and plasma assayed clearly influenced the level of PO activity, and was significantly reduced (P < 0.005) after treatment with 1-phenyl-2 thiourea, a specific PO inhibitor. This is the first report of the existence of PO in the hemolymph and plasma of a soft tick species. The regulation of PO activity and its precise role in soft tick immunity, particularly during the ecdysial phase, are interesting and need to be examined further.
  • K Maruta, T Yoshiga, C Katagiri, M Ochiai, S Tojo
    ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 50 2 97 - 106 2002年06月 [査読有り][通常論文]
     
    Biliverdin-binding vitellogenin (Vg) was purified from adult female hemolymph of the common cutworm, Spodoptera litura, by using gel filtration and ion exchange chromatographies. The molecular mass of the protein was 490 kDa and it was composed of two 188-kDa subunits. Three internal amino acid sequences obtained by digestion of the protein with lysylendopeptidase showed high similarity to those of Bombyx mori Vg, supporting the purified blue protein to be vitellogenin. latroscan analyses demonstrated the presence of biliverdin in Vg that occupied 2.4% of total lipid components. Among the lipids of Vg (9.5 mug total lipids per 100 mug protein), diacylglycerol was the most predominant, followed by phospholipid, hydrocarbons, and then triacylglycerol, while in biliverdin-binding proteins (BPs) purified from larval hemolymph (3.1 mug total lipids per 100 mug protein), phospholipid was the most abundant lipid followed by diacylglycerol; hydrocarbons and triacylglycerol were minor components. Vg was first defected in the hemolymph of female pupae one day before eclosion, but injection of 5 mug of methoprene into a 3-day-old pupa induced Vg in the hemolymph 4 days earlier than in the control. Methoprene also induced a faster decline in BP-A and BP-B titers in the hemolymph with a corresponding increase of the Vg titer. These results suggest that juvenile hormone (JH) induces not only vitellogenesis but also the uptake of these proteins by stimulating the metamorphosis of fat body during the pupal stage. (C) 2002 Wiley-liss, Inc.
  • A Yamanaka, T Ito, D Koga, T Sato, M Ochiai, K Endo
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 64 9 1978 - 1981 2000年09月 [査読有り][通常論文]
     
    The biliverdin-binding protein from the larval hemolymph of the swallowtail butterfly, Papilio xuthus L., was purified and characterized. The crude biliverdin-binding protein, obtained by ammonium sulfate fractionation, was purified in two steps, the first one by gel filtration chromatography and the second one by ion-exchange chromatography. The molecular mass of the purified protein was analyzed by SDS-polyacrylamide gel electrophoresis and estimated to be 21 kDa. The N-amino terminal sequence of P. xuthus biliverdin-binding protein analyzed up to the 19th residue showed that 42% of the amino acid sequence are sequence similarity to the bilin-binding protein from Pieris brassicae. These results suggest that the P. xuthus biliverdin-binding protein belongs to the insecticyanin-type.
  • Ochiai, M, Ashida, A
    J. Biol. Chem. 274 17 11854 - 11858 1999年 [査読有り][通常論文]
  • Satoh, D, Horii, A, Ochiai, M, Ashida, M
    J. Biol. Chem. 274 11 7441 - 7453 1999年 [査読有り][通常論文]
  • Hiraki, Y, Inoue, H, Iyama, K, Kamizono, A, Ochiai, M, Shukunami, C, Iijima, S, Suzuki, F, Kondo, J
    J. Biol. Chem. 272 51 32419 - 32426 1997年 [査読有り][通常論文]
  • Katsumi, Y, Kihara, H, Ochiai, M, Ashida, M
    Eur. J. Biochem. 228 3 870 - 877 1995年 [査読有り][通常論文]
  • Kawabata, T, Yasuhara, Y, Ochiai, M, Matsuura, S, Ashida, M
    Proc. Natl. Acad. Sci. USA 92 17 7774 - 7778 1995年 [査読有り][通常論文]
  • T SHIMOMURA, J KONDO, M OCHIAI, D NAKA, K MIYAZAWA, Y MORIMOTO, N KITAMURA
    JOURNAL OF BIOLOGICAL CHEMISTRY 268 30 22927 - 22932 1993年10月 [査読有り][通常論文]
     
    Hepatocyte growth factor activator (HGF activator) is a serine protease which converts single-chain HGF to the active two-chain form. HGF activator purified from human serum has a molecular mass of 34 kDa and consists of two chains held together by a disulfide bond. The nucleotide sequence of HGF activator cDNA shows that HGF activator is derived from the COOH-terminal region of a precursor of 655 amino acids by proteolytic cleavage of the bonds between Arg372 and Val373 and between Arg407 and Ile408 and that the precursor consists of multiple domains homologous to those observed in blood coagulation factor XII. In this study, we identified the precursor of HGF activator in human plasma using an enzyme-linked immunosorbent assay system. The precursor was purified from plasma by a five-step procedure. The purified precursor did not activate single-chain HGF. The precursor was efficiently cleaved in vitro by thrombin, at the bond between Arg407 and Ile408, in the presence of negatively charged substances. The cleaved precursor activated single-chain HGF. These findings led us to conclude that HGF activator is present in plasma as an inactive zymogen and that the zymogen is activated by the cleavage of the bond between Arg407 and Ile408 by thrombin. Characteristic structural domains in the NH2-terminal region of the zymogen may be involved in the binding of the zymogen to negatively charged substances, which stimulates the activation of the zymogen by thrombin.
  • Immunocytochemical localization of ß-1,3-glucan recognition protein in the silkworm, Bombyx mori.
    Ochiai, M, Niki, T, Ashida, M
    Cell Tissue Res. 268 431 - 437 1992年 [査読有り][通常論文]
  • Shimomura, T, Ochiai, M, Kondo, J, Morimoto, H, Teranishi, Y
    Cytotech. 8 219 - 229 1992年 [査読有り][通常論文]
  • M ASHIDA, M OCHIAI, T NIKI
    TISSUE & CELL 20 4 599 - 610 1988年 [査読有り][通常論文]
  • Purification of a ß-1,3-glucan recognition protein in the prophenoloxidase activating system from hemolymph of the silkworm, Bombyx mori.
    Ochiai, M, Ashida, M
    J. Biol. Chem. 263 24 12056 - 12062 1988年 [査読有り][通常論文]
  • Yoshida, H, Ochiai, M, Ashida, M
    Biochem. Biophys. Res. Commun. 141 1177 - 1184 1986年 [査読有り][通常論文]

書籍

  • 低温環境の科学事典
    落合 正則 (担当:分担執筆範囲:第7章 8. 昆虫の耐寒性)
    朝倉書店 2016年
  • 低温科学便覧
    落合 正則 (担当:分担執筆範囲:第14章 昆虫の環境ストレス応答)
    丸善出版 2015年

講演・口頭発表等

  • 昆虫の自然免疫とメラニン形成系  [通常講演]
    落合 正則
    公益財団法人 科学技術交流財団 第6回メラニン機能科学研究会 2019年03月 口頭発表(招待・特別)
  • Role of αC-domain in fibrinogen cryogelation  [通常講演]
    Ito Y, Dobashi T, Ochiai M, Toyama Y
    5th International Symposium of Gunma University Medical Innovation and 9th International Conference on Advanced Micro-Device Engineering 2018年12月 シンポジウム・ワークショップパネル(公募)
  • モンシロチョウ成虫における山口および札幌個体群間の毛状鱗粉  [通常講演]
    山中 明, 勇村悠介, 北沢千里, 落合正則
    日本動物学会第89回 2018年09月 口頭発表(一般)
  • フィブリノゲンクライオゲル形成に与えるカルシウムイオンの影響  [通常講演]
    伊藤 優吾, 外山 吉治, 土橋 敏明, 落合 正則
    第65回レオロジー討論会 2017年10月 口頭発表(一般)
  • カイコ幼虫における傷害関連分子パターン(DAMPs)の探索  [通常講演]
    石川 晴菜, 落合 正則
    日本動物学会第88回大会 2017年
  • ジャコウアゲハにおける短日および長日休眠蛹のホルモン応答性の比較  [通常講演]
    酒井 勇輝, 河 秀平, 北沢 千里, 落合 正則, 山中 明
    日本動物学会第88回大会 2017年
  • Development of the artificial multi-domain enzymes immobilized on the curdlan sheet at low cost.  [通常講演]
    Horiuchi M, Nagata A, Katahira M, Kobashigawa Y, Suzuki Y, Ochiai M
    The 8th International Symposium of Advanced Energy Science 2017年
  • モンシロチョウ成虫における毛状鱗粉形成の内分泌調節機構  [通常講演]
    勇村 悠介, 前平 剛史, 落合 正則, 北沢 千里, 山中 明
    第61回日本応用動物昆虫学会 2017年
  • Seasonal plasticity of the small copper butterfly Lycaena phlaeas daimio Seitz.  [通常講演]
    Yamanaka A, Masumoto Y, Abe N, Ochiai M, Kitazawa C
    XXV international Congress of Entomology 2016年
  • ルリタテハ成虫の季節型の違いによる翅の紋様と形状の解析  [通常講演]
    平木 佳奈, 山本 響, 北沢 千里, 落合 正則, 山中 明
    日本農芸化学会中四国支部第44回講演会 2016年
  • ヒメアカタテハの蛹体色の白色化に関する内分泌学的研究  [通常講演]
    長谷川 尋士, 林 絵里, 河崎 秀平, 森岡 律, 渕上 惣一郎, 北沢 千里, 落合 正則, 山中 明
    日本農芸化学会中四国支部第44回講演会 2016年
  • GRP-tagを用いた低コストなアゾ染料分解システムの構築  [通常講演]
    堀内 正隆, 鈴木 定彦, 落合 正則
    第88回日本生化学会大会 2015年
  • 数種のチョウ成虫の翅に生じる毛状鱗粉と季節型との関連性  [通常講演]
    村上 実菜子, 奥平 良弥, 益本 祐希, 安部 成美, 落合 正則, 北沢 千里, 山中 明
    第59回日本応用動物昆虫学会 2015年
  • Analysis of the gene products related to osseointegration in the early stage of titanium implantation  [通常講演]
    Horiuchi M, Horiuchi R, Ochiai M, Yokoyama A
    The 28th Annual Symposium of the Protein Society 2014年07月
  • 宿主アワヨトウ幼虫 からのカリヤコマユバチ脱出誘導因子の発見  [通常講演]
    砂山 貴志, 内川 雄貴, 落合 正則, 早川 洋一
    第58回日本応用動物昆虫学会 2014年
  • ベニシジミ成虫の毛状鱗粉形成に及ぼす日長と温度の影響  [通常講演]
    山中明, 益本祐希, 落合正則, 北沢千里
    第58回日本応用動物昆虫学会 2014年
  • シアル酸切除は高イオン強度下でのフィブリン凝集を加速する  [通常講演]
    第23回日本MRS年次大会 2013年
  • 高イオン強度下でのフィブリン凝集に対するシアル酸添加効果  [通常講演]
    第23回日本MRS年次大会 2013年
  • N結合型糖鎖とフィブリノーゲン分解産物との相互作用解析  [通常講演]
    第23回日本MRS年次大会 2013年
  • フィブリン重合におけるBβ鎖N末端領域の抑制効果  [通常講演]
    第23回日本MRS年次大会 2013年
  • ベニシジミ成虫の表現型可塑性について  [通常講演]
    平成25年度日本応用動物昆虫学会・日本昆虫学会中国支部号例会 2013年
  • カイコの微生物に対する生体防御機構  [通常講演]
    第65回日本衛生動物学会大会・病害動物の生理分子生物談話会 2013年
  • Possible involvement of chlorophyllase in a plant defense system  [通常講演]
    第54回日本植物生理学会 2013年
  • エビガラスズメ真皮細胞に存在する色素結合タンパク質凝集成分Xの性状について  [通常講演]
    日本蚕糸学会支部合同大会 2012年
  • ジャコウアゲハの帯糸の黒色化機構の解析  [通常講演]
    日本農芸化学会中四国支部大会 2012年
  • 微生物非感染時に自然免疫を活性化するサイトカイン  [通常講演]
    日本動物学会第83回大会 2012年
  • チタンインプラントにおけるオッセオインテグレーション獲得に関する遺伝子の網羅的解析  [通常講演]
    第25回歯科チタン学会学術講演会 2012年
  • Low-cost recombinant protein immobilization and purification system using β-1,3-glucan recognition protein from insect cells  [通常講演]
    第34回日本分子生物学会 2011年
  • オッセオインテグレーション獲得および非獲得ラットモデルにおける遺伝子の網羅的解析  [通常講演]
    第33回日本バイオマテリアル学会 2011年
  • Activation of the Prophenoloxidase Cascade in Hemocytes of the Locust, Locusta migratoria.  [通常講演]
    Sixth International Symposium on Molecular Insect Science 2011年
  • ジャコウアゲハの帯糸の黒色化に関わる因子  [通常講演]
    日本動物学会第82回大会 2011年
  • カイコのアポリポホリンIIIによるセクロピン抗菌活性の増強作用  [通常講演]
    日本動物学会第82回大会 2011年
  • フィブリン凝集に対するN-結合糖鎖の部分的切除効果  [通常講演]
    第60回高分子年次大会 2011年
  • フィブリン重合におけるフィブリノペプチドリリースの解析  [通常講演]
    第60回高分子年次大会 2011年
  • フィブリノゲンクライオゲル形成へのベタインの添加効果  [通常講演]
    第20回日本MRS学術シンポジウム 2010年
  • フィブリンゲル形成に対するプラスミン処理効果  [通常講演]
    第20回日本MRS学術シンポジウム 2010年
  • フィブリンゲル形成に対する糖鎖除去フィブリノゲンの混合効果  [通常講演]
    第20回日本MRS学術シンポジウム 2010年
  • GRP-tag融合蛋白質のカードランによる低コストなアフィニティー精製法の開発  [通常講演]
    第33回日本分子生物学会年会、第83回日本生化学会大会 合同大会 2010年
  • Deglycosylation Effect on the Fibrin Gel Formation  [通常講演]
    International Union of Materials Research Societies 11th International Conference 2010年
  • フィブリノーケンFragment-Xの凝集特性  [通常講演]
    第59回高分子討論会 2010年
  • 水晶振動子マイクロバランスを用いたフィブリノゲン及びフィブリン集合過程の測定  [通常講演]
    第33回日本バイオレオロジー学会年会 2010年
  • 昆虫の液性生体防御系と異物認識  [通常講演]
    第19回WSフォーラム タンパク質・ペプチド研究の現状と展望 2009年
  • カイコß-1,3-グルカン認識蛋白質の認識ドメインの立体構造  [通常講演]
    日本動物学会第80回大会 2009年
  • カイコのアポリポホリン-IIIの自然免疫における役割  [通常講演]
    日本動物学会第80回大会 2009年
  • フィブリノゲンクライオゲル形成に与える付加糖鎖の影響  [通常講演]
    第32回日本バイオレオロジー学会年会 2009年
  • フィブリノゲンクライオゲル形成に与えるフラグメントX及びD添加の影響  [通常講演]
    第32回日本バイオレオロジー学会年会 2009年
  • カイコ ßGRP/GNBP3のN末端ドメインの構造解析  [通常講演]
    第9回日本蛋白質科学会年会 2009年
  • カイコ ß-1,3-glucan recognition proteinの構造解析  [通常講演]
    第31回日本分子生物学会年会、第81回日本生化学会大会 合同大会 2008年
  • 日本動物学会第79回大会 2008年
  • ニュージーランド固有昆虫種ウェタのlipophorin  [通常講演]
    日本動物学会第79回大会 2008年
  • 精子形成細胞のカルシュウム・カルモジュリン結合性タンパク質;電子顕微鏡による観察  [通常講演]
    日本動物学会第79回大会 2008年
  • フィブリノーゲンクライオゲル形成に与える糖添加の効果  [通常講演]
    第30回バイオレオロジー学会年会 2007年 ポスター発表
  • Biochemical analysis of lipophorin in tree weta, Hemideina femorata.  [通常講演]
    2nd International Symposium on the Environmental Physiology of Ectotherms and Plants 2007年 ポスター発表
  • カイコのフェノ−ル酸化酵素前駆体活性化系におけるFactor H の役割  [通常講演]
    日本動物学会第78回大会 2007年
  • フィブリンゲル形成に及ぼす糖の異性体効果  [通常講演]
    第55回高分子学会年次大会 2006年
  • カイコフェノ−ル酸化酵素前駆体活性化系のモザイク構造をもつ新規プロテアーゼ前駆体  [通常講演]
    日本動物学会第77回大会 2006年
  • Functional role of prophenoloxidase cascade of the silkworm, Bombyx mori  [通常講演]
    Symposium on Pathogen Recognition and Signal Transduction in Innate Immunity 2005年
  • フィブリノーゲンとの相互作用における糖の異性体効果  [通常講演]
    第54回高分子学会年次大会 2005年 ポスター発表
  • NDSBsが蛋白質—蛋白質相互作用に及ぼす効果  [通常講演]
    第54回高分子学会年次大会 2005年
  • フェノ−ル酸化酵素前駆体活性化系のFactor Sの役割  [通常講演]
    文部科学省科学研究費特定領域研究(B)平成17年度公開シンポジウム「自然免疫による異物認識の分子基盤」 2005年
  • メラニン合成に関わるドーパクロム変換酵素の精製と一次構造  [通常講演]
    日本動物学会第76回大会 2005年 ポスター発表
  • カイコの新たなペプチドグリカン認識蛋白質  [通常講演]
    日本動物学会第76回大会 2005年 ポスター発表
  • カイコ幼虫体液のlipophorinと細菌リポ多糖の複合体形成  [通常講演]
    日本動物学会第76回大会 2005年 ポスター発表
  • フィブリノーゲンとの相互作用における二糖の異性体効果  [通常講演]
    第16回日本MRS学術シンポジウム 2005年 ポスター発表
  • 蛋白質の凝集に対するNDSBsの効果  [通常講演]
    第16回日本MRS学術シンポジウム 2005年 ポスター発表
  • フェノ−ル酸化酵素前駆体カスケ−ドにおけるグラム陰性菌結合蛋白質の役割  [通常講演]
    日本動物学会第75回大会 2004年 ポスター発表
  • フィブリンゲル形成過程に及ぼす糖類の影響 –フィブリノペプチド切断との関係  [通常講演]
    第53回高分子討論会 2004年 ポスター発表
  • DNA-コラーゲン複合体の構造制御について  [通常講演]
    第53回高分子討論会 2004年
  • フィブリンゲル形成におけるトロンビン酵素活性への糖類の影響  [通常講演]
    第53回高分子学会年次大会 2004年 ポスター発表
  • DNA-コラーゲン複合体ゲルの構造制御  [通常講演]
    第52回レオロジー討論会 2004年
  • Peptidoglycan-mediated activation of the proBAEEase pathway of the prophenoloxidase cascade  [通常講演]
    文部科学省科学研究費特定領域研究(B)平成16年度公開シンポジウム「自然免疫による異物認識の分子基盤」 2004年
  • DNA存在下におけるコラーゲン線維  [通常講演]
    第50回マトリックス研究会記念大会 2003年
  • カイコ幼虫斑紋形成に関与する顆粒フェノール酸化酵素(gPO)の精製とその性質  [通常講演]
    日本蚕糸学会第73回大会 2003年
  • コラーゲン-DNA複合体形成における添加DNAの形状の影響  [通常講演]
    第52回高分子学会年次大会 2003年
  • トロンビンによるフィブリノーゲン-フィブリン転換における添加物の効果  [通常講演]
    第52回高分子学会年次大会 2003年 ポスター発表
  • An overview of the prophenoloxidase cascade of insects  [通常講演]
    文部科学省科学研究費特定領域研究(B)平成15年度公開シンポジウム「自然免疫による異物認識の分子基盤」 2003年
  • A new serine protease zymogen of the silkworm prophenoloxidase cascade  [通常講演]
    文部科学省科学研究費特定領域研究(B)平成15年度公開シンポジウム「自然免疫による異物認識の分子基盤」 2003年
  • カイコのフェノ−ル酸化酵素前駆体活性化系の未同定因子の検出とproBAEEase活性化経路  [通常講演]
    日本動物学会第74回大会 2003年
  • フェノール酸化酵素前駆体(proPO)カスケードから得られた新しい活性型プロテアーゼ  [通常講演]
    日本動物学会第74回大会 2003年
  • The peptidoglycan-mediated pathway of the silkworm prophenoloxidase cascade  [通常講演]
    The 16th Naito Conference on Innate Immunity in Medicine and Biology [I] 2003年 ポスター発表
  • Structure and Formation Process of Collagen–DNA Complex  [通常講演]
    The 8th Pacific Polymer Conference 2003年 ポスター発表
  • カイコのペプチドグリカン認識蛋白質ファミリー  [通常講演]
    日本動物学会第73回大会 2002年 ポスター発表
  • フェノール酸化酵素前駆体活性化系の新たに単利された構成因子(プロテアーゼ前駆体)  [通常講演]
    日本動物学会第73回大会 2002年 ポスター発表
  • 精子形成細胞のカルモジュリンと関連するヒートショックプロテイン複合体について  [通常講演]
    日本動物学会第73回大会 2002年 ポスター発表
  • コラーゲン-DNAコンポジットの形成について  [通常講演]
    第51回高分子討論会 2002年
  • Effect of DNA structure on collagen fibrillogenesis  [通常講演]
    第39回ペプチド討論会 2002年
  • フェノール酸化酵素前駆体カスケードのペプチドグリカン活性化経路  [通常講演]
    文部科学省科学研究費特定領域研究(B)平成14年度公開シンポジウム「自然免疫による異物認識の分子基盤」 2002年
  • トロンビンによるフィブリノーゲン-フィブリン転換における糖類の影響  [通常講演]
    第14回日本MRS学術シンポジウム 2002年 ポスター発表
  • カイコのβ-1,3-グルカン認識蛋白質II  [通常講演]
    日本比較免疫学会第13回学術集会 2001年 ポスター発表
  • カイコ体液中のフェノール酸化酵素前駆体カスケードの液性生体防御における役割  [通常講演]
    文部科学省科学研究費特定領域研究(B)平成13年度公開シンポジウム「自然免疫による異物認識の分子基盤」 2001年
  • A new component of the prophenoloxidase cascade, ß-1,3-glucan recognition protein II from the silkworm, Bombyx mori  [通常講演]
    XXI international Congress of Entomology 2000年 ポスター発表
  • Prophenoloxidase cascade of the silkworm, Bombyx mori  [通常講演]
    XXI international Congress of Entomology 2000年
  • グラム陰性菌結合蛋白質はフェノ−ル酸化酵素前駆体カスケ−ドの構成因子である  [通常講演]
    日本動物学会第71回大会 2000年 ポスター発表
  • 家蚕血漿中のセリンプロテアーゼ(BAEEase)によるspatzleの限定分解  [通常講演]
    日本動物学会第71回大会 2000年 ポスター発表
  • カイコのβ-1,3-グルカン認識蛋白質II  [通常講演]
    日本動物学会第70回大会 1999年
  • 家蚕血漿中に存在するセリンプロテアーゼ前駆体(proBAEEase)の性状解析  [通常講演]
    日本動物学会第70回大会 1999年
  • カイコ卵のフェノール酸化酵素前駆体活性化酵素に関する研究  [通常講演]
    日本動物学会第70回大会 1999年
  • 昆虫フェノール酸化酵素の接着性に関与するアミノ酸配列について  [通常講演]
    日本動物学会第70回大会 1999年
  • Pro-phenol oxidase cascade in insect hemolymph and cuticle  [通常講演]
    Third International Symposium on Molecular Insect Science, Utah, USA 1998年
  • カイコの細菌感染によるペプチドグリカン認識蛋白質の誘導合成  [通常講演]
    日本動物学会第69回大会 1998年
  • 家蚕血液から外皮へ移行したフェノール酸化酵素前駆体が受けている修飾について  [通常講演]
    日本動物学会第69回大会 1998年
  • 家蚕血漿中に存在するセリンプロテアーゼ前駆体のcDNAクローニング  [通常講演]
    日本動物学会第69回大会 1998年
  • カイコの初期発生におけるフェノール酸化酵素前駆体の局在  [通常講演]
    日本動物学会第69回大会 1998年

その他活動・業績

  • カイコの微生物感染によるメラニン形成と異物認識
    落合 正則 蚕糸・昆虫バイオテック 84 (3) 195 -204 2015年 [査読有り][招待有り]

共同研究・競争的資金等の研究課題

  • ßGRPとカードランによる簡便で低コストな組換えタンパク質固定化法の研究
    日本学術振興会:科学研究費補助金
    研究期間 : 2012年 -2013年 
    代表者 : 堀内 正隆
  • 細胞性免疫に関与する昆虫サイトカインの微生物感染による活性化機構の解析
    日本学術振興会:科学研究費補助金
    研究期間 : 2011年 -2013年 
    代表者 : 落合 正則
  • 自然免疫による異物認識の分子基盤の総括と評価
    日本学術振興会:科学研究費補助金
    研究期間 : 2004年 -2005年 
    代表者 : 落合 正則
  • 昆虫のフェノール酸化酵素前駆体活性化系構成因子の同定
    日本学術振興会:科学研究費補助金
    研究期間 : 2003年 -2005年 
    代表者 : 落合 正則
  • 自然免疫を制御するシグナル伝達カスケードとネットワーク形成
    日本学術振興会:科学研究費補助金
    研究期間 : 2005年 
    代表者 : 倉田 祥一郎
  • 昆虫のフェノール酸化酵素前駆体活性化系の新規構成因子の同定
    内藤記念科学振興財団:特定研究助成金
    研究期間 : 2004年 
    代表者 : 落合 正則
  • 昆虫の液性及び細胞性生体防御におけるプロテアーゼカスケードの役割
    日本学術振興会:科学研究費補助金
    研究期間 : 2001年 -2003年 
    代表者 : 芦田 正明
  • 昆虫のフェノール酸化酵素カスケード活性化の分子機構と生体防御における役割
    日本学術振興会:科学研究費補助金
    研究期間 : 1997年 -2001年 
    代表者 : 芦田 正明
  • フェノ-ル酸化酵素前駆体活性化系の時間的・空間的制御機構
    日本学術振興会:科学研究費補助金
    研究期間 : 1996年 -1997年 
    代表者 : 芦田 正明
  • 細菌細胞壁構成成分の微量検出・定量試薬の開発
    日本学術振興会:科学研究費補助金
    研究期間 : 1994年 -1996年 
    代表者 : 芦田 正明
  • マラリア媒介蚊の非感受精機構に係わる生体防御因子の分子生物学的解析
    日本学術振興会:科学研究費補助金
    研究期間 : 1996年 
    代表者 : 芦田 正明
  • 昆虫体液の異物認識蛋白質に関する研究-異物認識の分子機構と生体防御機構-
    日本学術振興会:科学研究費補助金
    研究期間 : 1995年 
    代表者 : 落合 正則
  • 昆虫の生体防御機構とフェノール酸化酵素前駆体活性化カスケードの解析
    日本学術振興会:科学研究費補助金
    研究期間 : 1993年 
    代表者 : 落合 正則
  • Insect immunity and prophenoloxidase cascade
    研究期間 : 1993年

教育活動情報

主要な担当授業

  • 環境分子生物学特論Ⅰ
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 環境科学院
    キーワード : 微生物学、極限環境、分子生物学、生態学、分子昆虫学、哺乳類、発生生理化学、生化学 Microbiology,extremophiles,molecular biology,ecology,molecular Entomology, development and biochemistry in mammals
  • 分子生物学基礎論
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 環境科学院
    キーワード : 分子生物学,転写,翻訳,微生物の群集構造解析,微生物の呼吸活性,電子顕微鏡観察,遺伝子操作,バイオフィルム形成,植物ストレス耐性,バイオセンサー,昆虫免疫系,昆虫体表脂質 molecular biology, transcription, translation, bacterial culture, bacterial community structure analysis, bacterial respiration activity, electron microscopic observation, genetic manipulation, biofilm formation, stress tolerance of plants, biosensor, insect immune system, insect body surface lipids


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