研究者データベース

THAPA JEEWAN(タパ ジーワン)
人獣共通感染症国際共同研究所 バイオリソース部門
助教

基本情報

所属

  • 人獣共通感染症国際共同研究所 バイオリソース部門

職名

  • 助教

ホームページURL

科研費研究者番号

  • 40837449

J-Global ID

研究キーワード

  • Infectious diseases   Bacteriology   

研究分野

  • ライフサイエンス / 細菌学

職歴

  • 2018年10月 - 現在 北海道大学
  • 2017年10月 - 2018年09月 Hokkaido University Research Center for Zoonosis Control JSPS Postdoctoral Fellow
  • 2009年07月 - 2013年03月 National Trust for Nature Conservation, Nepal Veterinary Officer
  • 2007年12月 - 2009年06月 Tribhuvan University Institute of Agriculture and Animal Science Research Associate

学歴

  • 2013年10月 - 2017年09月   Hokkaido University   School of Veterinary Medicine   PhD
  • 2008年07月 - 2010年12月   Tribhuvan University   Institute of Agriculture and Animal Science   Master of Veterinary Science

研究活動情報

論文

  • Jeewan Thapa, Kent Hashimoto, Saori Sugawara, Ryoya Tsujikawa, Torahiko Okubo, Shinji Nakamura, Hiroyuki Yamaguchi
    Microbes and infection 2020年05月19日 [査読有り][通常論文]
     
    Chlamydia trachomatis LGV (CtL2) causes systemic infection and proliferates in lymph nodes as well as genital tract or rectum producing a robust inflammatory response, presumably leading to a low oxygen environment. We therefore assessed how CtL2 growth in immortal human epithelial cells adapts to hypoxic conditions. Assessment of inclusion forming units, the quantity of chlamydial 16S rDNA, and inclusion size showed that hypoxia promotes CtL2 growth. Under hypoxia, HIF-1α was stabilized and p53 was degraded in infected cells. Moreover, AKT was strongly phosphorylated at S473 by CtL2 infection. This activation was significantly diminished by LY-294002, a PI3K-AKT inhibitor, which decreased the number of CtL2 progeny. HIF-1α stabilizers (CoCl2, desferrioxamine) had no effect on increasing CtL2 growth, indicating no autocrine impact of growth factors produced by HIF-1α stabilization. Furthermore, in normoxia, CtL2 infection changed the NAD+/NADH ratio of cells with increased gapdh expression; in contrast, under hypoxia, the NAD+/NADH ratio was the same in infected and uninfected cells with high and stable expression of gapdh, suggesting that CtL2-infected cells adapted better to hypoxia. Together, these data indicate that hypoxia promotes CtL2 growth in immortal human epithelial cells by activating the PI3K-AKT pathway and maintaining the NAD+/NADH ratio with stably activated glycolysis.
  • Jeewan Thapa, Takanori Watanabe, Mana Isoba, Torahiko Okubo, Kiyotake Abe, Kunihiro Minami, Hiroyuki Yamaguchi
    BMC infectious diseases 20 1 53 - 53 2020年01月16日 [査読有り][通常論文]
     
    BACKGROUND: This study was conducted to understand the molecular epidemiology of circulating Chlamydia trachomatis (Ct) strains in Sapporo, Japan. METHODS: A total of 713 endocervical samples collected from April 2016 to March 2019 were screened for Ct. The obtained Ct positive samples were analyzed by ompA genotyping and multilocus sequence analysis (MLSA). RESULTS: Eighty-three (11.6%) samples were positive for Ct plasmid DNA. Sequence analysis of the ompA gene from the 61 positive cases revealed eight genotypes: F (40.9%), E (19.6%), D (14.7%), G (9.8%), H (6.5%), I (3.2%), K (3.2%), and J (1.6%). The globally dominant genotype E and F strains were highly conserved with 13 ompA genetic variants being detected, whereas genotype D strains were the most diverse. Genetic characterization of D strains revealed that D1 genetic variants may be potentially specific to Sapporo. MLSA revealed 13 unique sequence types (STs) including four novel STs from 53 positive samples, with the globally dominant STs 39 and 19 being predominant. STs 39, 34, and 21 were exclusively associated with genotypes E and F indicating their global dominance. Novel ST70 and ST30 were specifically associated with genotype D. CONCLUSION: Our study has revealed the circulation of genetically diverse Ct strains in the women population of Sapporo, Japan. We suggest identifying a transmission network of those successful strains and implementing public health prevention strategies to control the spread of Ct in Sapporo.
  • Effect of thermal control of dry fomites on regulating the survival of human pathogenic bacteria responsible for nosocomial infections
    Tomoko Shimoda, Torahiko Okubo, Yoshiki Enoeda, Rika Yano, Jeewan Thapa, Shinji Nakamura, Hiroyuki Yamaguchi
    PLoS ONE e0226952 2019年12月 [査読有り][通常論文]
     
    ヒト病原細菌(ブドウ球菌、大腸菌ほか)が乾燥した素材(床材やステンレス)表面でどの程度生存するかを検証するとともに、その生存性に影響する因子として温度が大きく関わることを明らかにしました。単純な環境要因ですが、手摺やドアノブさらにベット柵など乾燥面の温度を調節することで院内感染の制御へと応用できるかもしれません。
  • Screening of hospital-manhole sewages using MacConkey agar with cefotaxime reveals extended-spectrum β-lactamase-producing Escherichia coli.
    Okubo T, Hasegawa T, Fukuda A, Thapa J, Usui M, Tamura Y, Yamaguchi H
    Int J Antimicrob Agents In press 2019年08月 [査読有り][通常論文]
  • Direct detection of Mycobacterium tuberculosis in clinical samples by a dry methyl green loop-mediated isothermal amplification (LAMP) method.
    Thapa J, Maharjan B, Malla M, Fukushima Y, Poudel A, Pandey BD, Hyashida K, Gordon SV, Nakajima C, Suzuki Y
    Tuberculosis 117 1 - 6 2019年07月 [査読有り][通常論文]
  • Shah Y, Poudel A, Maharjan B, Thapa J, Yamaguchi T, Diab HM, Pandey BD, Solo E, Isoda N, Suzuki Y, Nakajima C
    Transactions of the Royal Society of Tropical Medicine and Hygiene 2019年01月 [査読有り][通常論文]
  • Sarad Paudel, Susan K. Mikota, Jeewan Thapa, Konstantin P. Lyashchenko, Kamal P. Gairhe, Ishwari Prasad Dhakal, Naresh Subedi, Bhagwan Maharjan, Suraj Subedi, Gretchen E. Kaufman, Toshio Tsubota
    European Journal of Wildlife Research 64 6 2018年12月 [査読有り][通常論文]
  • Genetic diversity and distribution dynamics of multidrug-resistant Mycobacterium tuberculosis isolates in Nepal
    Maharjan B, Nakajima C, Isoda N, Thapa J, Poudel A, Shah Y, Yamaguchi T, Shrestha B, Hoffmann H, Avsar K, Shrestha A, Gordon SV, Suzuki Y
    Scientific Reports 8 16634 1 - 10 2018年11月 [査読有り][通常論文]
  • Nan Aye Thida Oo, Lai Lai San, Jeewan Thapa, Khin Saw Aye, Wah Wah Aung, Chie Nakajima, Yasuhiko Suzuki
    Tuberculosis 111 8 - 13 2018年07月01日 [査読有り][通常論文]
     
    Numerous studies report that mutations of rpsL (encoding the S12 protein), rrs (encoding 16S rRNA) and gidB (encoding rRNA methyltransferase) are responsible for conferring resistance to streptomycin (STR), which is usually used in both multidrug-resistant tuberculosis (MDR-TB) treatments and re-treatments in Myanmar. The aim of this study was to explore the variation and frequency of mutations in rpsL, rrs and gidB in 141 STR-resistant MDR-TB isolates from Myanmar. Most isolates belonged to the Beijing genotype (105, 74.5%). Moreover, mutations in rpsL were identified in 69.5% (98/141) of the STR-resistant isolates, where the most prevalent (92.0%, 90/98) and significantly associated mutation with the Beijing genotype (P < 0.001) was Lys43Arg. Fifteen different mutations in gidB were found in 16.3% (23/141) of the isolates, and most of them were novel mutations. Moreover, based on our results, we suggest A276C nucleotide substitution in gidB as a phylogenetic marker for the Beijing family in Myanmar. Sequence analysis of rpsL, rrs and gidB with a sensitivity of 83.7% satisfactorily predicted STR resistance in Myanmar isolates. However, in 16.3% (23/141) of the isolates, none of the examined genes showed mutation. Hence, further studies are strongly recommended to elucidate other possible resistance mechanisms. The present findings may be useful in developing molecular STR susceptibility assays, which in turn could contribute to develop TB treatments and control strategies in Myanmar.
  • Z. Rahim, J. Thapa, Y. Fukushima, A. G. M. van der Zanden, S. V. Gordon, Y. Suzuki, C. Nakajima
    TRANSBOUNDARY AND EMERGING DISEASES 64 6 1965 - 1969 2017年12月 [査読有り][通常論文]
     
    Mycobacterium orygis, commonly known as the oryx bacillus and a newly proposed Mycobacterium tuberculosis complex subspecies, was isolated from 18 cattle in a dairy farm and two captured rhesus monkeys in a zoo in Bangladesh. All the infected animals had tuberculosis lesions in their lungs, suggesting transmission and infection with M.orygis by an airborne route. The 20 isolates were analysed using a range of conventional and molecular typing methods, and RD-deletion typing and sequencing of selected genes confirmed the isolates as M.orygis. Multiple-locus variable-number tandem repeat analysis (MLVA) allowed the isolates to be divided into three clusters based on the relatedness of their MLVA profiles. The two monkey isolates shared the same MLVA pattern with 15 of the cattle isolates, whereas the remaining three cattle isolates had different patterns, even though the latter animals had been kept in the same dairy farm. The diversity observed among isolates may suggest the bacteria have been established in this area for a long period. This study along with other recent findings that report the detection of M.orygis from animals as well as humans originating from South Asia potentially indicate endemic distribution of M.orygis in South Asia.
  • Yogendra Shah, Bhagwan Maharjan, Jeewan Thapa, Ajay Poudel, Hassan Mahmoud Diab, Basu Dev Pandey, Eddie S. Solo, Norikazu Isoda, Yasuhiko Suzuki, Chie Nakajima
    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES 63 13 - 20 2017年10月 [査読有り][通常論文]
     
    Objectives: Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) poses a major public health problem in Nepal. Although it has been reported as one of the dominant genotypes of MTB in Nepal, little information on the Central Asian Strain (CAS) family is available, especially isolates related to multidrug resistance (MDR) cases. This study aimed to elucidate the genetic and epidemiological characteristics of MDR CAS isolates in Nepal. Methods: A total of 145 MDR CAS isolates collected in Nepal from 2008 to 2013 were characterized by spoligotyping, mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) analysis, and drug resistance-associated gene sequencing. Results: Spoligotyping analysis showed CAS1_Delhi SIT26 as predominant (60/145, 41.4%). However, by combining spoligotyping and MIRU-VNTR typing, it was possible to successfully discriminate all 145 isolates into 116 different types including 18 clusters with 47 isolates (clustering rate 32.4%). About a half of these clustered isolates shared the same genetic and geographical characteristics with other isolates in each cluster, and some of them shared rare point mutations in rpoB that are thought to be associated with rifampicin resistance. Conclusions: Although the data obtained show little evidence that large outbreaks of MDR-TB caused by the CAS family have occurred in Nepal, they strongly suggest several MDR-MTB transmission cases. (C) 2017 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
  • Jeewan Thapa, Sarad Paudel, Amir Sadaula, Yogendra Shah, Bhagwan Maharjan, Gretchen E. Kaufman, Deborah McCauley, Kamal P. Gairhe, Toshio Tsubota, Yasuhiko Suzuki, Chie Nakajima
    EMERGING INFECTIOUS DISEASES 22 3 570 - 572 2016年03月 [査読有り][通常論文]
  • Jeewan Thapa, Chie Nakajima, Bhagwan Maharjan, Ajay Poudel, Yasuhiko Suzuki
    JAPANESE JOURNAL OF VETERINARY RESEARCH 63 3 151 - 158 2015年08月 [査読有り][通常論文]
     
    Mycobacterium orygis, a new member of the Mycobacterium tuberculosis complex, was isolated from a captive spotted deer (Axis axis) and a blue bull (Boselaphus tragocamelus) in Nepal. Analyses by spoligotyping, mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing, region of difference and single nucleotide polymorphism of genes gyrB, mmpL6, TbD1, PPE55 and Rv2042c confirmed the isolates as M. orygis. Moreover, analyses by spoligotyping (SIT587) as well as MIRU-VNTR showed that the isolates shared a similar pattern with many reported isolates. From previous and the present studies, it can be inferred that South Asia is one of the endemic regions for M. orygis. Further investigation including a larger sample size and different host interaction will help to understand the ecology and epidemiology of M. orygis in Nepal.
  • Sarad Paudel, Susan K. Mikota, Chie Nakajima, Kamal P. Gairhe, Bhagwan Maharjan, Jeewan Thapa, Ajay Poudel, Michito Shimozuru, Yasuhiko Suzuki, Toshio Tsubota
    TUBERCULOSIS 94 3 287 - 292 2014年05月 [査読有り][通常論文]
     
    Mycobacterium tuberculosis was cultured from the lung tissues of 3 captive elephants in Nepal that died with extensive lung lesions. Spoligotyping, TbD1 detection and multi-locus variable number of tandem repeat analysis (MLVA) results suggested 3 isolates belonged to a specific lineage of Indo-Oceanic clade, EAI5 SIT 138. One of the elephant isolates had a new synonymous single nucleotide polymorphism (SNP) T231C in the gyrA sequence, and the same SNP was also found in human isolates in Nepal. MLVA results and transfer history of the elephants suggested that 2 of them might be infected with M. tuberculosis from the same source. These findings indicated the source of M. tuberculosis infection of those elephants were local residents, presumably their handlers. Further investigation including detailed genotyping of elephant and human isolates is needed to clarify the infection route and eventually prevent the transmission of tuberculosis to susceptible hosts. (C) 2014 Elsevier Ltd. All rights reserved.

書籍

  • Wildlife Tuberculosis: An Emerging Threat for Conservation in South Asia
    Jeewan Thapa, Chie Nakajima, Kamal P. Gairhe, Bhagwan Maharjan, Sarad Paudel, Yogendra Shah, Susan K. Mikota, Gretchen E. Kaufman, Deborah McCauley, Toshio Tsubota, Stephen V. Gordon, Yasuhiko Suzuki (担当:共著範囲:English)
    www.intechopen.com 2017年03月

その他活動・業績

  • One Healthアプローチを通じた細菌性感染症の制御 病原性微生物の特性に関する新規分析法の開発(Development of new methods for the characterization of bacterial pathogens)
    中島 千絵, 鈴木 定彦, Thapa Jeewan 日本細菌学雑誌 74 (1) 5 -5 2019年03月 [査読無し][通常論文]
  • ループ仲介等温増幅法を用いた人畜共通感染性結核の迅速検出法(Rapid detection of zoonotic tuberculosis using Loop mediated isothermal amplification)
    Kapalamula Thoko, Thapa Jeewan, 中島 千絵, Akapelwa Mwangala, Gordon Stephen V, 鈴木 定彦 日本細菌学雑誌 74 (1) 67 -67 2019年03月 [査読無し][通常論文]
  • メチルグリーンを用いたループ仲介等温増幅法による結核の診断(Diagnosis of tuberculosis by methyl green based loop-mediated isothermal amplification method)
    Thapa Jeewan, Maharjan Bhagwan, 福島 由華里, Malla Meena, Poudel Ajay, Pandey Basu Dev, 中島 千絵, 鈴木 定彦 日本細菌学雑誌 73 (1) 64 -64 2018年02月 [査読無し][通常論文]
  • 南アジアはMycobacterium orygisの風土病地域である(South Asia is an endemic region for Mycobacterium orygis)
    Thapa Jeewan, 中島 千絵, Rahim Zeaur, Paudel Sarad, Shah Yogendra, Maharian Bhagwan, Poudel Ajay, Gairhe Kamal Prasad, 坪田 敏男, 鈴木 定彦 日本細菌学雑誌 71 (1) 159 -159 2016年02月 [査読無し][通常論文]
  • THAPA Jeewan, NAKAJIMA Chie, SUZUKI Yasuhiko 日本細菌学雑誌 70 (1) 200 2015年02月25日 [査読無し][通常論文]
  • THAPA Jeewan, NAKAJIMA Chie, SUZUKI Yasuhiko 日本細菌学雑誌 69 (1) 141 2014年02月25日 [査読無し][通常論文]

教育活動情報

主要な担当授業

  • 微生物学実習Ⅰ
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 医学部
  • 微生物学実習Ⅰ
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 消毒方法 滅菌方法 培地の作成 無菌操作 染色 分離培養 純培養 菌種同定 
  • 微生物学実習Ⅱ
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 医学部
  • 微生物学実習Ⅱ
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 組織 協調性 責任 社会生活 規律 細菌検査 細菌用培地 細菌培養 細菌同定 薬剤感受性試験
  • 臨床微生物学
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 病原微生物 細菌 真菌 ウイルス 薬剤耐性機序 病原因子 感染症 病態形成 検査方法 同定方法 疫学調査 治療
  • 医学英語
    開講年度 : 2019年
    課程区分 : 学士課程
    開講学部 : 医学部
    キーワード : 医学英語、英語論文、英文実験プロトコール


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