研究者データベース

三上 秀治(ミカミ ヒデハル)
電子科学研究所 生命科学研究部門
教授

基本情報

所属

  • 電子科学研究所 生命科学研究部門

職名

  • 教授

学位

  • 博士(理学)(2006年03月 東京大学)

J-Global ID

プロフィール

  • 2006年 東京大学大学院 理学系研究科 物理学専攻 博士課程修了後、(株)日立製作所 研究員(途中、カリフォルニア大学アーバイン校 客員研究員を兼任、2012年11月-2013年11月)、東京大学大学院理学系研究科化学専攻 助教を経て2020年6月より北海道大学 電子科学研究所 教授。2017年10月よりJSTさきがけ研究者(兼任)。専門は光物理、光応用工学全般。これまでに量子光学・量子情報科学、光ディスク・光通信技術、生体観察向け非線形光学顕微鏡(二光子、SHG、CARS)の研究を行ってきた。現在は生体内の高速現象の観察やリアルタイム3D観察、高スループットイメージングフローサイトメトリなどへの応用を目指した超高速蛍光イメージング技術を開発している。


研究分野

  • ライフサイエンス / 生物物理学 / バイオイメージング
  • ナノテク・材料 / 光工学、光量子科学

研究活動情報

論文

  • A Isozaki, Y Nakagawa, M H Loo, Y Shibata, N Tanaka, D L Setyaningrum, J-W Park, Y Shirasaki, H Mikami, D Huang, H Tsoi, C T Riche, T Ota, H Miwa, Y Kanda, T Ito, K Yamada, O Iwata, K Suzuki, S Ohnuki, Y Ohya, Y Kato, T Hasunuma, S Matsusaka, M Yamagishi, M Yazawa, S Uemura, K Nagasawa, H Watarai, D Di Carlo, K Goda
    Science advances 6 22 eaba6712 - eaba6712 2020年05月 [査読有り][通常論文]
     
    Droplet microfluidics has become a powerful tool in precision medicine, green biotechnology, and cell therapy for single-cell analysis and selection by virtue of its ability to effectively confine cells. However, there remains a fundamental trade-off between droplet volume and sorting throughput, limiting the advantages of droplet microfluidics to small droplets (<10 pl) that are incompatible with long-term maintenance and growth of most cells. We present a sequentially addressable dielectrophoretic array (SADA) sorter to overcome this problem. The SADA sorter uses an on-chip array of electrodes activated and deactivated in a sequence synchronized to the speed and position of a passing target droplet to deliver an accumulated dielectrophoretic force and gently pull it in the direction of sorting in a high-speed flow. We use it to demonstrate large-droplet sorting with ~20-fold higher throughputs than conventional techniques and apply it to long-term single-cell analysis of Saccharomyces cerevisiae based on their growth rate.
  • Hideharu Mikami, Makoto Kawaguchi, Chun-Jung Huang, Hiroki Matsumura, Takeaki Sugimura, Kangrui Huang, Cheng Lei, Shunnosuke Ueno, Taichi Miura, Takuro Ito, Kazumichi Nagasawa, Takanori Maeno, Hiroshi Watarai, Mai Yamagishi, Sotaro Uemura, Shinsuke Ohnuki, Yoshikazu Ohya, Hiromi Kurokawa, Satoshi Matsusaka, Chia-Wei Sun, Yasuyuki Ozeki, Keisuke Goda
    Nature communications 11 1 1162 - 1162 2020年03月06日 [査読有り][通常論文]
     
    By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology, microbiology, immunology, hematology, and stem cell biology. However, the performance and utility of IFC are severely limited by the fundamental trade-off between throughput, sensitivity, and spatial resolution. Here we present an optomechanical imaging method that overcomes the trade-off by virtually freezing the motion of flowing cells on the image sensor to effectively achieve 1000 times longer exposure time for microscopy-grade fluorescence image acquisition. Consequently, it enables high-throughput IFC of single cells at >10,000 cells s-1 without sacrificing sensitivity and spatial resolution. The availability of numerous information-rich fluorescence cell images allows high-dimensional statistical analysis and accurate classification with deep learning, as evidenced by our demonstration of unique applications in hematology and microbiology.
  • Akihiro Isozaki, Hideharu Mikami, Hiroshi Tezuka, Hiroki Matsumura, Kangrui Huang, Marino Akamine, Kotaro Hiramatsu, Takanori Iino, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Yan Li, Yuta Nakagawa, Shinsuke Ohnuki, Tadataka Ota, Yong Qian, Shinya Sakuma, Takeichiro Sekiya, Yoshitaka Shirasaki, Nobutake Suzuki, Ehsen Tayyabi, Tsubasa Wakamiya, Muzhen Xu, Mai Yamagishi, Haochen Yan, Qiang Yu, Sheng Yan, Dan Yuan, Wei Zhang, Yaqi Zhao, Fumihito Arai, Robert E. Campbell, Christophe Danelon, Dino Di Carlo, Kei Hiraki, Yu Hoshino, Yoichiroh Hosokawa, Mary Inaba, Atsuhiro Nakagawa, Yoshikazu Ohya, Minoru Oikawa, Sotaro Uemura, Yasuyuki Ozeki, Takeaki Sugimura, Nao Nitta, Keisuke Goda
    Lab on a Chip 20 13 2263 - 2273 2020年 [査読有り][通常論文]
     

    The upgraded version of intelligent image-activated cell sorting (iIACS) has enabled higher-throughput and more sensitive intelligent image-based sorting of single live cells from heterogeneous populations.

  • Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda
    Nature protocols 14 11 3273 - 3273 2019年11月 [査読有り][通常論文]
     
    An amendment to this paper has been published and can be accessed via a link at the top of the paper.
  • Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda
    Nature protocols 14 8 2370 - 2415 2019年08月 [査読有り][通常論文]
     
    Intelligent image-activated cell sorting (iIACS) is a machine-intelligence technology that performs real-time intelligent image-based sorting of single cells with high throughput. iIACS extends beyond the capabilities of fluorescence-activated cell sorting (FACS) from fluorescence intensity profiles of cells to multidimensional images, thereby enabling high-content sorting of cells or cell clusters with unique spatial chemical and morphological traits. Therefore, iIACS serves as an integral part of holistic single-cell analysis by enabling direct links between population-level analysis (flow cytometry), cell-level analysis (microscopy), and gene-level analysis (sequencing). Specifically, iIACS is based on a seamless integration of high-throughput cell microscopy (e.g., multicolor fluorescence imaging, bright-field imaging), cell focusing, cell sorting, and deep learning on a hybrid software-hardware data management infrastructure, enabling real-time automated operation for data acquisition, data processing, intelligent decision making, and actuation. Here, we provide a practical guide to iIACS that describes how to design, build, characterize, and use an iIACS machine. The guide includes the consideration of several important design parameters, such as throughput, sensitivity, dynamic range, image quality, sort purity, and sort yield; the development and integration of optical, microfluidic, electrical, computational, and mechanical components; and the characterization and practical usage of the integrated system. Assuming that all components are readily available, a team of several researchers experienced in optics, electronics, digital signal processing, microfluidics, mechatronics, and flow cytometry can complete this protocol in ~3 months.
  • Simple, stable, compact implementation of frequency-division-multiplexed microscopy by inline interferometry
    Hiroshi Kanno, Hideharu Mikami, Yasin Kaya, Yasuyuki Ozeki, Keisuke Goda
    Optics Letters 44 3 467 - 470 2019年 [査読有り][通常論文]
  • Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki, Hideya Fukuzawa, Misa Hase, Takeshi Hayakawa, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Kenichi Koizumi, SangWook Lee, Cheng Lei, Ming Li, Takanori Maeno, Satoshi Matsusaka, Daichi Murakami, Atsuhiro Nakagawa, Yusuke Oguchi, Minoru Oikawa, Tadataka Ota, Kiyotaka Shiba, Hirofumi Shintaku, Yoshitaka Shirasaki, Kanako Suga, Yuta Suzuki, Nobutake Suzuki, Yo Tanaka, Hiroshi Tezuka, Chihana Toyokawa, Yaxiaer Yalikun, Makoto Yamada, Mai Yamagishi, Takashi Yamano, Atsushi Yasumoto, Yutaka Yatomi, Masayuki Yazawa, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Yasuyuki Ozeki, Keisuke Goda
    Cell 175 1 266 - 276 2018年09月20日 [査読有り][通常論文]
     
    A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.
  • Lei Cheng, Kobayashi Hirofumi, Wu Yi, Li Ming, Isozaki Akihiro, Yasumoto Atsushi, Mikami Hideharu, Ito Takuro, Nitta Nao, Sugimura Takeaki, Yamada Makoto, Yatomi Yutaka, Di Carlo Dino, Ozeki Yasuyuki, Goda Keisuke
    NATURE PROTOCOLS 13 7 1603 - 1631 2018年07月 [査読有り][通常論文]
  • Miura Taichi, Mikami Hideharu, Isozaki Akihiro, Ito Takuro, Ozeki Yasuyuki, Goda Keisuke
    BIOMEDICAL OPTICS EXPRESS 9 7 3424 - 3433 2018年07月01日 [査読有り][通常論文]
  • Hideharu Mikami, Jeffrey Harmon, Hirofumi Kobayashi, Syed Hamad, Yisen Wang, Osamu Iwata, Kengo Suzuki, Takuro Ito, Yuri Aisaka, Natsumaro Kutsuna, Kazumichi Nagasawa, Hiroshi Watarai, Yasuyuki Ozeki, Keisuke Goda
    Optica 5 2 117 - 126 2018年02月20日 [査読有り][通常論文]
     
    Laser-scanning confocal fluorescence microscopy is an indispensable tool for biomedical research by virtue of its high spatial resolution. Its temporal resolution is equally important, but is still inadequate for many applications. Here we present a confocal fluorescence microscope that, for the first time to our knowledge, surpasses the highest possible frame rate constrained only by the fluorescence lifetime of fluorophores (typically a few to several nanoseconds). This microscope is enabled by integrating a broadband, spatially distributed, dual-frequency comb or spatial dual-comb and quadrature amplitude modulation for optimizing spectral efficiency into frequency-division multiplexing with single-pixel photodetection for signal integration. Specifically, we demonstrate confocal fluorescence microscopy at a record high frame rate of 16,000 frames/s. To show its broad biomedical utility, we use the microscope to demonstrate 3D volumetric confocal fluorescence microscopy of cellular dynamics at 104 volumes/s and confocal fluorescence imaging flow cytometry of hematological and microalgal cells at up to 2 m/s.
  • Hideharu Mikami, Jeffrey Harmon, Yasuyuki Ozeki, Keisuke Goda
    Proceedings of SPIE - The International Society for Optical Engineering 10251 2017年 [査読有り][通常論文]
     
    We present methods of fluorescence confocal microscopy that enable unprecedentedly high frame rate of > 10,000 fps. The methods are based on a frequency-division multiplexing technique, which was originally developed in the field of communication engineering. Specifically, we achieved a broad bandwidth (∼400 MHz) of detection signals using a dual-AOD method and overcame limitations in frame rate, due to a scanning device, by using a multi-line focusing method, resulting in a significant increase in frame rate. The methods have potential biomedical applications such as observation of sub-millisecond dynamics in biological tissues, in-vivo three-dimensional imaging, and fluorescence imaging flow cytometry.
  • Mihaela Balu, Hideharu Mikami, Jue Hou, Eric O. Potma, Bruce J. Tromberg
    BIOMEDICAL OPTICS EXPRESS 7 11 4375 - 4387 2016年11月 [査読有り][通常論文]
     
    We present a multiphoton microscope designed for mesoscale imaging of human skin. The system is based on two-photon excited fluorescence and second-harmonic generation, and images areas of similar to 0.8x0.8 mm(2) at speeds of 0.8 fps (800x800 pixels; 12 frame averages) for high signal-to-noise ratio, with lateral and axial resolutions of 0.5 mu m and 3.3 mu m, respectively. The main novelty of this instrument is the design of the scan head, which includes a fast galvanometric scanner, optimized relay optics, a beam expander and high NA objective lens. Computed aberrations in focus are below the Marechal criterion of 0.07. rms for diffraction-limited performance. We demonstrate the practical utility of this microscope by ex-vivo imaging of wide areas in normal human skin. (C) 2016 Optical Society of America
  • Hideharu Mikami, Liang Gao, Keisuke Goda
    NANOPHOTONICS 5 4 497 - 509 2016年10月 [査読有り][通常論文]
     
    High-speed optical imaging is an indispensable technology for blur-free observation of fast transient dynamics in virtually all areas including science, industry, defense, energy, and medicine. High temporal resolution is particularly important for microscopy as even a slow event appears to occur "fast" in a small field of view. Unfortunately, the shutter speed and frame rate of conventional cameras based on electronic image sensors are significantly constrained by their electrical operation and limited storage. Over the recent years, several unique and unconventional approaches to high-speed optical imaging have been reported to circumvent these technical challenges and achieve a frame rate and shutter speed far beyond what can be reached with the conventional image sensors. In this article, we review the concepts and principles of such ultrafast optical imaging methods, compare their advantages and disadvantages, and discuss an entirely new class of applications that are possible using them.
  • Hideharu Mikami, Liang Gao, Keisuke Goda
    Nanophotonics 5 4 441 - 453 2016年09月01日 [査読有り][通常論文]
     
    High-speed optical imaging is an indispensable technology for blur-free observation of fast transient dynamics in virtually all areas including science, industry, defense, energy, and medicine. High temporal resolution is particularly important for microscopy as even a slow event appears to occur "fast" in a small field of view. Unfortunately, the shutter speed and frame rate of conventional cameras based on electronic image sensors are significantly constrained by their electrical operation and limited storage. Over the recent years, several unique and unconventional approaches to high-speed optical imaging have been reported to circumvent these technical challenges and achieve a frame rate and shutter speed far beyond what can be reached with the conventional image sensors. In this article, we review the concepts and principles of such ultrafast optical imaging methods, compare their advantages and disadvantages, and discuss an entirely new class of applications that are possible using them.
  • Mihaela Balu, Hideharu Mikami, Jue Hou, Eric O. Potma, Bruce J. Tromberg
    Progress in Biomedical Optics and Imaging - Proceedings of SPIE 9712 2016年 [査読有り][通常論文]
     
    Clinical examination crucially relies on the ability to quickly examine large tissue areas and rapidly zoom in to regions of interest. Skin lesions often show irregularity in color and appearance in general, especially when they start to progress towards malignancy. Large field of view (FOV) and automatic translation of the imaging area are critical in the assessment of the entire lesion. Imaging of limited FOVs of the lesion can easily result in false negative diagnosis. We present a multiphoton microscope based on two-photon excited fluorescence and second-harmonic generation that images FOVs of about 0.8 mm2 (without stitching adjacent FOVs) at speeds of 10 frames/second (800 x 800 pixels) with lateral and axial resolutions of 0.5 μm and 2.5 μm, respectively. The main novelty of this instrument is the design of the scan head, which includes a fast galvanometric scanner, relay optics, a beam expander and a high NA objective lens. We optimized the system based on the Olympus 25x, 1.05NA water immersion lens, that features a long working distance of 1 mm. Proper tailoring of the beam expander, which consists of the scan and tube lens elements, enables scaling of the FOV. The design criteria include a flat wavefront of the beam, minimum field curvature, and suppressed spherical aberrations. All aberrations in focus are below the Marechal criterion of 0.07λ rms for diffraction-limited performance. We demonstrate the practical utility of this microscope by ex-vivo imaging of wide FOVs in normal human skin.
  • Mihaela Balu, Hideharu Mikami, Jue Hou, Eric O. Potma, Bruce J. Tromberg
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XVI 9712 2016年 [査読有り][通常論文]
     
    Clinical examination crucially relies on the ability to quickly examine large tissue areas and rapidly zoom in to regions of interest. Skin lesions often show irregularity in color and appearance in general, especially when they start to progress towards malignancy. Large field of view (FOV) and automatic translation of the imaging area are critical in the assessment of the entire lesion. Imaging of limited FOVs of the lesion can easily result in false negative diagnosis. We present a multiphoton microscope based on two-photon excited fluorescence and second-harmonic generation that images FOVs of about 0.8 mm(2) (without stitching adjacent FOVs) at speeds of 10 frames/second (800 x 800 pixels) with lateral and axial resolutions of 0.5 mu m and 2.5 mu m, respectively. The main novelty of this instrument is the design of the scan head, which includes a fast galvanometric scanner, relay optics, a beam expander and a high NA objective lens. We optimized the system based on the Olympus 25x, 1.05NA water immersion lens, that features a long working distance of 1 mm. Proper tailoring of the beam expander, which consists of the scan and tube lens elements, enables scaling of the FOV. The design criteria include a flat wavefront of the beam, minimum field curvature, and suppressed spherical aberrations. All aberrations in focus are below the Marechal criterion of 0.07 lambda rms for diffraction-limited performance. We demonstrate the practical utility of this microscope by ex-vivo imaging of wide FOVs in normal human skin.
  • Hideharu Mikami, Hirofumi Kobayashi, Yisen Wang, Syed Hamad, Yasuyuki Ozeki, Keisuke Goda
    HIGH-SPEED BIOMEDICAL IMAGING AND SPECTROSCOPY: TOWARD BIG DATA INSTRUMENTATION AND MANAGEMENT 9720 2016年 [査読有り][通常論文]
     
    Fluorescence imaging using radiofrequency-tagged emission (FIRE) is an emerging technique that enables higher imaging speed (namely, temporal resolution) in fluorescence microscopy compared to conventional fluorescence imaging techniques such as confocal microscopy and wide-field microscopy. It works based on the principle that it uses multiple intensity-modulated fields in an interferometric setup as excitation fields and applies frequency-division multiplexing to fluorescence signals. Unfortunately, despite its high potential, FIRE has limited imaging speed due to two practical limitations: signal bandwidth and signal detection efficiency. The signal bandwidth is limited by that of an acousto-optic deflector (AOD) employed in the setup, which is typically 100-200 MHz for the spectral range of fluorescence excitation (400-600 nm). The signal detection efficiency is limited by poor spatial mode-matching between two interfering fields to produce a modulated excitation field. Here we present a method to overcome these limitations and thus to achieve higher imaging speed than the prior version of FIRE. Our method achieves an increase in signal bandwidth by a factor of two and nearly optimal mode matching, which enables the imaging speed limited by the lifetime of the target fluorophore rather than the imaging system itself. The higher bandwidth and better signal detection efficiency work synergistically because higher bandwidth requires higher signal levels to avoid the contribution of shot noise and amplifier noise to the fluorescence signal. Due to its unprecedentedly high-speed performance, our method has a wide variety of applications in cancer detection, drug discovery, and regenerative medicine.
  • Hideharu Mikami, Hirofumi Kobayashi, Syed Hamad, Yasuyuki Ozeki, Keisuke Goda
    2016 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO) 2016年 [査読有り][通常論文]
     
    We propose and demonstrate ultrafast fluorescence confocal microscopy at a record high frame rate of 32,000 fps. This is made possible by frequency-division multiplexing with multi-line focusing.
  • Hideharu Mikami, Manabu Shiozawa, Masataka Shirai, Koichi Watanabe
    OPTICS EXPRESS 23 13 17217 - 17222 2015年06月 [査読有り][通常論文]
     
    Compact and fully collinear light source for multiplex coherent anti-Stokes Raman scattering (CARS) microscopy was proposed and demonstrated. It consists of only a microchip laser, a short photonic crystal fiber, and a longpass filter. It offers performance of sensitivity, bandwidth, and spectral resolution suitable for biomedical applications, especially covering the entire fingerprint region (500-1800 cm(-1)). It can be readily implemented by a commercially available microchip laser and a photonic crystal fiber. It has great potential to expand the utility of CARS microscopy to a wide variety of fields such as endoscopy. (C) 2015 Optical Society of America
  • Hideharu Mikami, Manabu Shiozawa, Masataka Shirai, Koichi Watanabe
    OPTICS EXPRESS 23 13 17217 - 17222 2015年06月 [査読有り][通常論文]
     
    Compact and fully collinear light source for multiplex coherent anti-Stokes Raman scattering (CARS) microscopy was proposed and demonstrated. It consists of only a microchip laser, a short photonic crystal fiber, and a longpass filter. It offers performance of sensitivity, bandwidth, and spectral resolution suitable for biomedical applications, especially covering the entire fingerprint region (500-1800 cm(-1)). It can be readily implemented by a commercially available microchip laser and a photonic crystal fiber. It has great potential to expand the utility of CARS microscopy to a wide variety of fields such as endoscopy. (C) 2015 Optical Society of America
  • Hideharu Mikami, Manabu Shiozawa, Masataka Shirai, Koichi Watanabe
    OPTICS EXPRESS 23 3 2872 - 2878 2015年02月 [査読有り][通常論文]
     
    A compact light source module for ultrabroadband coherent anti-Stoke Raman scattering (CARS) microscopy was developed. It mainly consists of a nanosecond microchip laser, a photonic crystal fiber for Stokes light generation, and a single mode polarization maintaining fiber for pump light propagation. It is alignment-free and relatively low-cost compared with previous light sources of CARS microscopy. By using an assembled module, we successfully observed an ultrabroadband CARS spectrum and a CARS image of a murine adipocyte. The module is expected to greatly spread the CARS microscopy to various fields by its extreme easiness to handle. (C) 2015 Optical Society of America
  • Hideharu Mikami, Manabu Shiozawa, Masataka Shirai, Koichi Watanabe
    OPTICS EXPRESS 23 4 5300 - 5311 2015年02月 [査読有り][通常論文]
     
    We propose a simple quantitative index for coherent anti-Stoke Raman scattering (CARS) spectroscopy and microscopy. Unlike previous similar indices, it can be applied to samples with arbitrary molar concentration, and it is robust against environmental change. Concentrations of aqueous hydrogen peroxide solution and lipid concentration distribution in a live murine adipocyte were successfully quantified by the new index. The index can be obtained in a broad range of CARS setups and it is readily applicable to quantitative CARS microscopy for deep inspection of samples such as biological specimens. (C) 2015 Optical Society of America
  • Hideharu Mikami, Manabu Shiozawa, Masataka Shirai, Koichi Watanabe
    OPTICS EXPRESS 23 3 2872 - 2878 2015年02月 [査読有り][通常論文]
     
    A compact light source module for ultrabroadband coherent anti-Stoke Raman scattering (CARS) microscopy was developed. It mainly consists of a nanosecond microchip laser, a photonic crystal fiber for Stokes light generation, and a single mode polarization maintaining fiber for pump light propagation. It is alignment-free and relatively low-cost compared with previous light sources of CARS microscopy. By using an assembled module, we successfully observed an ultrabroadband CARS spectrum and a CARS image of a murine adipocyte. The module is expected to greatly spread the CARS microscopy to various fields by its extreme easiness to handle. (C) 2015 Optical Society of America
  • Hideharu Mikami, Manabu Shiozawa, Masataka Shirai, Koichi Watanabe
    OPTICS EXPRESS 23 4 5300 - 5311 2015年02月 [査読有り][通常論文]
     
    We propose a simple quantitative index for coherent anti-Stoke Raman scattering (CARS) spectroscopy and microscopy. Unlike previous similar indices, it can be applied to samples with arbitrary molar concentration, and it is robust against environmental change. Concentrations of aqueous hydrogen peroxide solution and lipid concentration distribution in a live murine adipocyte were successfully quantified by the new index. The index can be obtained in a broad range of CARS setups and it is readily applicable to quantitative CARS microscopy for deep inspection of samples such as biological specimens. (C) 2015 Optical Society of America
  • Hideharu Mikami, Koichi Watanabe
    Japanese Journal of Applied Physics 52 9 2013年09月 [査読有り][通常論文]
     
    Microholographic optical data storage is a promising candidate for next-generation optical disc systems because of its potential huge capacity. We proposed and demonstrated a new novel multiplexing technique for microholographic optical data storage using spatial modes of an optical beam to enhance data capacity. Numerical simulation was performed to validate the proposal and it was estimated that the net increase of data capacity by applying two- and four-mode multiplexing with Hermite-Gaussian modes was 1.3 and 1.7 times, respectively. A proof-of-principle experiment for two-mode multiplexing was performed by using spatial modes equivalent to Laguerre-Gaussian modes and it was demonstrated that signal output was selectively obtained by choosing an appropriate spatial mode for reference light of phase-diversity homodyne detection. Because the proposed technique can be applied in conjunction with other multiplexing and multilevel techniques, it is expected to greatly increase the possible applications of future optical disc systems. © 2013 The Japan Society of Applied Physics.
  • Hideharu Mikami, Takahiro Kurokawa, Koichi Watanabe
    JAPANESE JOURNAL OF APPLIED PHYSICS 51 8 2012年08月 [査読有り][通常論文]
     
    Homodyne detection is a detection technique that significantly amplifies optical signals. It is useful for the readout of multilayer optical discs, where the signal level is quite low. The improvement of optical disc readout signal quality by homodyne detection has been demonstrated experimentally. However, homodyne detection requires a complicated optical system, which causes large-scale implementation and high cost, which prevents practical use of the technique in optical disc systems. In this report, we demonstrated compact implementation of homodyne detection. We proposed and assembled two types of optical module for homodyne detection. The sizes of the modules were 10 x 30 and 10 x 40 mm(2), which were comparable to that of the current optical pickup. Jitter of the attenuated Blu-ray Disc (TM) recordable (BD-R) readout signals was improved from 15 to 7.8% by applying one of the modules. (C) 2012 The Japan Society of Applied Physics
  • Atsushi Kikukawa, Hideharu Mikami, Tatsuro Ide, Kentaro Osawa, Koichi Watanabe
    JAPANESE JOURNAL OF APPLIED PHYSICS 51 8 2012年08月 [査読有り][通常論文]
     
    The feasibility of increasing the read data transfer rate (DTR) by introducing optical phase multilevel recording technology was investigated using computer simulations. The signals read back from phase marks suffer from strong intersymbol interference (ISI) when the phase marks are recorded with a linear symbol density comparable to that of current optical disc systems; thus, the partial response most-likely (PRML) method is essential. The increase in the decoder size is a serious problem when applying the PRML method to multilevel signal decoding; however, it was shown that this can be resolved by applying run-length limited (RLL) modulations. With these, it was shown that it is possible to decode 4-ary phase-modulated signals with satisfactory performance using PRML. Therefore, we conclude that it is possible to at least double the read DTR by introducing the optical phase multilevel recording technology. (C) 2012 The Japan Society of Applied Physics
  • Hideharu Mikami, Kentaro Osawa, Eriko Tatsu, Koichi Watanabe
    JAPANESE JOURNAL OF APPLIED PHYSICS 51 8 2012年08月 [査読有り][通常論文]
     
    The microhologram is one of the most promising candidates for the next generation optical disc. It can achieve a huge data capacity because it is suitable for multilayer recording. However, it cannot increase the data transfer rate because of its comparable areal recording density with conventional optical discs. Moreover, the signal level in the readout process of this scheme is in general very low, which prevents its practical use. Recently, an optical phase multilevel scheme that overcomes the above drawbacks of the microhologram has been proposed. The scheme uses an optical phase as stored information, which enables data readout with an extremely high signal-to-noise ratio and multilevel modulation. In this report, recording and readout processes of the proposed scheme are demonstrated experimentally. Four-level phase modulation was successfully regenerated from weak 30 nW microholograms with errors of +7.0/-12.2 degrees, suggesting that a further increase in the number of levels is possible. (C) 2012 The Japan Society of Applied Physics
  • Tatsuro Ide, Kentaro Osawa, Hideharu Mikami, Koichi Watanabe
    JAPANESE JOURNAL OF APPLIED PHYSICS 51 8 2012年08月 [査読有り][通常論文]
     
    We theoretically and experimentally studied the effects of phase-diversity homodyne detection on the interlayer crosstalk of a multilayer optical disc by comparison with those of conventional intensity detection. From analytical studies, we clarified the differences in interlayer crosstalk of both detections. Interlayer crosstalk consists of two noises, the intensity of the stray light N-1 and the interference between the signal and stray light N-2. The noise N-1, which is dominant between these two, drastically decreases with layer spacing in phase-diversity homodyne detection owing to mismatch in the phase distribution between reference and stray light compared with that in intensity detection. Simulations and experiments on a dual-layer Blu-ray Disc (TM) having a layer spacing less than 10 mu m demonstrated that phase-diversity homodyne detection provided higher tolerance to interlayer crosstalk than the conventional intensity detection. (C) 2012 The Japan Society of Applied Physics
  • Tatsuro Ide, Hideharu Mikami, Kentaro Osawa, Koichi Watanabe
    JAPANESE JOURNAL OF APPLIED PHYSICS 50 9 2011年09月 [査読有り][通常論文]
     
    An optical phase multilevel recording technique using a microholographic system and phase-diversity homodyne detection for enhancement of optical disc capacity is investigated. In this technique, multilevel phase signals are stored as the fringe shifts along the optical axis and recovered from the arctangent of two homodyne-detected signals. For comparison, phase signals from Blu-ray Disc read-only memory (BD-ROM) and Blu-ray Disc recordable (BD-R) media obtained by phase-diversity homodyne detection are experimentally evaluated. From the experimental results, we demonstrated that phase-diversity homodyne detection is useful for detecting the phase signal modulation of the signal beam from an optical disc. Furthermore, simulation results on microholograms indicate that phase signals from the microholograms are much more stable despite the variety of their sizes than those from BD-ROM. These results demonstrate the potential of this multilevel recording method. (C) 2011 The Japan Society of Applied Physics
  • Kentaro Osawa, Hideharu Mikami, Takahiro Kurokawa, Koichi Watanabe
    OPTICAL DATA STORAGE 2010 7730 2010年 [査読有り][通常論文]
     
    A system using homodyne detection to read out signals from optical discs was simplified by using a high-coherence laser light source. The quality of the readout signal with the simplified system was substantially greater than with conventional detection.
  • Hideharu Mikami, Kentaro Osawa, Koichi Watanabe
    OPTICAL DATA STORAGE 2010 7730 2010年 [査読有り][通常論文]
     
    A novel multi-level scheme using optical phase is proposed. It overcomes drawbacks in conventional multi-level schemes and greatly enhances capacity and transfer rate of microholographic optical discs. We demonstrate its feature of high signal-to-noise ratio.
  • Hideharu Mikami, Takeshi Shimano, Takahiro Kurokawa, Tatsuro Ide, Jiro Hashizume, Koichi Watanabe, Harukazu Miyamoto
    JAPANESE JOURNAL OF APPLIED PHYSICS 48 3 2009年03月 [査読有り][通常論文]
     
    We experimentally demonstrated the amplification of optical disk readout signals by homodyne detection. This technique uses optical interference to amplify the signals. We further applied phase-diversity detection to reliably obtain the amplified readout signal. The optical system was carefully designed so that a sufficiently amplified readout signal can be obtained. In particular, we applied a corner cube prism as a reflection mirror to achieve sufficient stability of the interferometric optical system. We experimentally demonstrated a 3.6 times amplification of a Blu-ray Disc readout signal. The estimated signal-to-noise ratio (SNR) improvement for an assumed eight-layer optical disk readout signals by applying homodyne detection on the basis of the observed amplification was +7.9dB, which significantly enables reliable readout of recorded signals. The present technique will be essential for the real commercialization of next-generation multilayer optical disk because of its outstanding ability of SNR improvement. (C) 2009 The Japan Society of Applied Physics
  • Takahiro Kurokawa, Hideharu Mikami, Hiroyuki Minemura, Tatsuro Ide, Koichi Watanabe, Harukazu Miyamoto
    JAPANESE JOURNAL OF APPLIED PHYSICS 48 3 2009年03月 [査読有り][通常論文]
     
    We developed a signal-readout system suitable for optical pickups with a homodyne detection scheme, which is used to amplify signal lights using optical interference. The system consists of an optical-signal detection circuit and a readout-signal generator. The optical-signal detection circuit, which contains pairs of photodiodes connected in series, successfully cancels out large DC components arising from a reference light. This made it possible to avoid the output signal saturation of current-to-voltage amplifiers and raise the upper limit of signal gain. The readout-signal generator generates a readout signal on the basis of the phase-diversity method, which stabilizes readout signal amplitude. By using this system, a signal gain of 3.6 was obtained on a commercially available disc. (C) 2009 The Japan Society of Applied Physics
  • Hideharu Mikami, Takahiro Kurokawa, Koichi Watanabe, Harukazu Miyamoto
    2009 OPTICAL DATA STORAGE TOPICAL MEETING 46 - 48 2009年 [査読有り][通常論文]
  • Hideharu Mikami, Takayoshi Kobayashi
    Physical Review A - Atomic, Molecular, and Optical Physics 75 2 2007年02月22日 [査読有り][通常論文]
     
    We report an experimental demonstration of remote preparation of an arbitrary pure qutrit state. A qutrit is encoded in a two-photon (biphoton) polarization state. Prepared states are characterized by quantum state tomography. Experimental imperfection is discussed and evaluated by an experimentally obtained density matrix. The present scheme can develop into generation of an arbitrary two-photon triplet state and accordingly to an essential multiparty quantum communication protocol. Generalization to higher order which enables remote preparation of an arbitrary qudit state and several typical multiphoton entangled states is also shown. © 2007 The American Physical Society.
  • Hideharu Mikami, Takayoshi Kobayashi
    PHYSICAL REVIEW A 75 2 2007年02月 [査読有り][通常論文]
     
    We report an experimental demonstration of remote preparation of an arbitrary pure qutrit state. A qutrit is encoded in a two-photon (biphoton) polarization state. Prepared states are characterized by quantum state tomography. Experimental imperfection is discussed and evaluated by an experimentally obtained density matrix. The present scheme can develop into generation of an arbitrary two-photon triplet state and accordingly to an essential multiparty quantum communication protocol. Generalization to higher order which enables remote preparation of an arbitrary qudit state and several typical multiphoton entangled states is also shown.
  • YM Li, H Mikami, HB Wang, T Kobayashi
    PHYSICAL REVIEW A 72 6 2005年12月 [査読有り][通常論文]
     
    In this paper, we study the theory of spontaneous parametric down-conversion pumped by a short coherent pulse. It is shown that when single spatial mode filters and narrow band optical filters are used to filter the output state of parametric down-conversion, the postselected state of parametric down-conversion can be approximately described by a simple single-mode theory.
  • H Mikami, YM Li, K Fukuoka, T Kobayashi
    PHYSICAL REVIEW LETTERS 95 15 150404  2005年10月 [査読有り][通常論文]
     
    We propose and demonstrate a new scheme of high-efficiency generation of the three-photon polarization-entangled W state, which is a typical three-qubit entangled state. The high efficiency has enabled the first full characterization of the state by quantum state tomography. We have analyzed the obtained state and observed its nature of tripartite entanglement and robustness of entanglement.
  • Hideharu Mikami, Yongmin Li, Kyosuke Fukuoka, Takayoshi Kobayashi
    IQEC, International Quantum Electronics Conference Proceedings 2005 1012 - 1013 2005年 [査読有り][通常論文]
     
    We propose and demonstrate a new scheme of high-efficiency generation of the three-photon polarization-entangled W state, which is a typical three-qubit entangled state. The obtained state is characterized using quantum state tomography.
  • Hideharu Mikami, Yongmin Li, Takayoshi Kobayashi
    Physical Review A - Atomic, Molecular, and Optical Physics 70 5 A 1 - 52308 2004年11月 [査読有り][通常論文]
     
    Two methods of generating the four-photon W state were proposed. The methods utilized parametric down-conversion processes, linear optical elements, and commercial photon detectors, which are feasible under current technology. The methods could be used to generate the three-photon W state, the three-photon Greenberger-Horne-Zeilinger state, and the three photon maximally entangled photon number state by simply changing some experimental components or the parameters. It was shown that the methods could be developed into methods that generate a general n-photon W state.
  • H Mikami, YM Li, T Kobayashi
    PHYSICAL REVIEW A 70 5 2004年11月 [査読有り][通常論文]
     
    We propose two interesting methods of generating the four-photon W state. These methods use parametric down-conversion processes, linear optical elements, and commercial photon detectors, which are readily feasible under current technology. They can also be used to generate the three-photon W state, the three-photon Greenberger-Horne-Zeilinger state, and the three-photon maximally entangled photon-number state (a typical photon-number entanglement state) by simply changing some experimental components or their parameters. Moreover, assuming we have photon number-resolving detectors, these methods can develop into methods that generate a general n-photon W state. They are expected to become powerful tools for experimental investigations of multipartite entanglement and its applications to quantum information processing.

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