研究者データベース

ELEWA YASER HOSNY ALI(エレワ ヤセル ホスニー アリ)
獣医学研究院 獣医学部門 基礎獣医科学分野
助教

基本情報

所属

  • 獣医学研究院 獣医学部門 基礎獣医科学分野

職名

  • 助教

学位

  • 博士(獣医学)(Zagazig大学)

ホームページURL

科研費研究者番号

  • 30782221

J-Global ID

研究キーワード

  • Mucosa associated lymphoid tissue   Bone disorder   autoimmunity   Reproductive disorders and infertility   Kidney diseases   Respiratory diseases   Lymphocytes   Mediatinal fat-associated Lymphoid clusters   Innate immunity   

研究分野

  • ライフサイエンス / 細胞生物学

職歴

  • 2017年02月 - 現在 Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University Assistant professor
  • 2016年04月 - 現在 Department of Histology, Faculty of Veterinary Medicine Zagazig University, Zagazig, Egypt Associate professor
  • 2014年10月 - 2016年09月 Laboratory of Anatomy, Graduate school of Veterinary Medicine Hokkaido University, Japan JSPS post doc.
  • 2011年04月 - 2016年03月 Department of Histology, Faculty of Veterinary Medicine Zagazig University, Zagazig, Egypt Lecturer
  • 2011年07月 - 2012年01月 Laboratory of Anatomy, Graduate school of Veterinary Medicine Hokkaido University, Japan post Doctoral researcher
  • 2008年10月 - 2010年09月 Laboratory of Anatomy, Graduate school of Veterinary Medicine Hokkaido University, Japan Teaching volunteer
  • 2006年07月 - 2007年01月 Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences Niigata University, Nigata, Japan Visiting researcher
  • 2002年05月 - 2006年02月 Department of Histology, Faculty of Veterinary Medicine Zagazig University, Zagazig, Egypt Demonstrator

学歴

  • - 現在   Faculty of Veterinary Medicine, Zagazig University

所属学協会

  • Japanese Society for promotion and Sciences (JSPS)- Alumni, Egypt   Egyptian Society of Veterinary medical sciences   Egyptian Society of Histology and Cytology   日本解剖学会   日本獣医学会   

研究活動情報

論文

  • Nesma Ibraheim El-Naseery, Yaser Hosny Ali Elewa, Osamu Ichii, Yasuhiro Kon
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 220 9 - 20 2018年11月 [査読無し][通常論文]
     
    The current study was conducted on a menopause rat model induced by ovariectomy to assess the histological and immunohistochemical alterations in the parotid glands and to verify the efficiency of human umbilical cord derived-mesenchymal stromal cell (hUCB-MSCs) in treating this condition. Eighteen adult female rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX) and OVX injected with hUCB-MSCs (OVX+hUCB-MSCs). At 3months post-ovariectomy, the salivary flow rate and size of the parotid glands were measured. The parotid glands were histologically investigated via H&E stained sections. Furthermore, immunohistochemical analysis for human CD105, human CD34, proliferating cell nuclear antigen (PCNA), single strand DNA (ssDNA), caspase 3, aquaporin (AQP)1, α-smooth muscle actin (α-SMA) and mouse CD34 were performed. The OVX group showed interstitial hemorrhage, dispersed acini and intracytoplasmic vacuoles in the acinar cells. Furthermore, immunohistochemical staining revealed a significant decrement in the number of ssDNA positive apoptotic cells, but a significant increment of PCNA positive proliferating cells, AQP1 positive blood capillaries, α-SMA positive myoepithelial cells and endogenous CD34 positive hematopoietic progenitor cells in the OVX+hUCB-MSCs group as compared with the OVX group. These findings suggest a potential regenerative therapy of MSCs to injured parotid gland structures. However, further investigations are required to illustrate the mechanism of hUCB-MSCs mediated parotid gland regeneration.
  • Osamu Ichii, Teppei Nakamura, Taro Horino, Akira Yabuki, Yaser H A Elewa, Yasuhiro Kon
    The American journal of pathology 188 9 2120 - 2138 2018年09月 [査読無し][通常論文]
     
    The distal tubule (DT) helps regulate blood pressure and electrolytes. We describe a novel, autosomal recessive, morphofunctional DT abnormality in inbred mice evident as columnar alternations and age-related cystic changes. This abnormality developed in both sexes of DBA/2Cr. Similar phenotypes were observed in A/J, C3H/He, DBA/1J, and FVB/N strains, but not in AKR/N, BALB/c, or C57BL/6N strains. In DBA/2Cr, abnormal DT localized to straight and convoluted segments and showed IL-36α DT injury marker expression. However, DT epithelial proliferation, examined by bromodeoxyuridine incorporation, was not remarkably altered with the progression of abnormality. Abnormal DT epithelial cells in DBA/2Cr displayed elongated primary cilia, loose intercellular adhesions, and numerous vesicles with altered localization of CD9, Na+/K+ATPase, and E-cadherin, indicating altered cell function, adhesion, and polarity. DBA/2Cr-type D12Mit182-D12Mit83 was identified as a candidate locus designated DBA/2 renal cyst (drecy). Within drecy, the gene regulated by estrogen in breast cancer protein (Greb1) transcript variant 2 was significantly up-regulated in DBA/2Cr kidney versus C57BL/6N. Greb1 localized to DT cytoplasm in C57BL/6 and to cytoplasm and nucleus in DBA/2Cr. Greb1-overexpressing M-1 kidney cells showed an altered epithelial-mesenchyme phenotype. B6.D2-(D12Mit182-D12Mit83) congenic mice carrying drecy did not show DT abnormalities, whereas DBA/2Cr × B6.D2-(D12Mit182-D12Mit83) mice did. Identification of this novel DT abnormality regulated by a DBA/2Cr mouse chromosome 12-derived locus and additional genetic factors improve the understanding of DT pathogenesis.
  • Samah R. Khalil, Hesham A. Khalifa, Sabry M. Abdel-Motal, Hesham H. Mohammed, Yaser H.A. Elewa, Hend Atta Mahmoud
    Ecotoxicology and Environmental Safety 157 255 - 265 2018年08月15日 [査読無し][通常論文]
     
    Heavy metals are well known as environmental pollutants with hazardous impacts on human and animal health because of their wide industrial usage. In the present study, the role of Spirulina platensis in reversing the oxidative stress-mediated brain injury elicited by lead acetate exposure was evaluated. In order to accomplish this aim, rats were orally administered with 300 mg/kg bw Spirulina for 15 d, before and simultaneously with an intraperitoneal injection of 50 mg/kg bw lead acetate [6 injections through the two weeks]. As a result, the co-administration of Spirulina with lead acetate reversed the most impaired open field behavioral indices however, this did not happen for swimming performance, inclined plane, and grip strength tests. In addition, it was observed that Spirulina diminished the lead content that accumulated in both the blood and the brain tissue of the exposed rats, and reduced the elevated levels of oxidative damage indices, and brain proinflammatory markers. Also, because of the Spirulina administration, the levels of the depleted biomarkers of antioxidant status and interleukin–10 in the lead-exposed rats were improved. Moreover, Spirulina protected the brain tissue (cerebrum and cerebellum) against the changes elicited by lead exposure, and also decreased the reactivity of HSP70 and Caspase–3 in both cerebrum and cerebellum tissues. Collectively, our findings demonstrate that Spirulina has a potential use as a food supplement in the regions highly polluted with heavy metals.
  • Teppei Nakamura, Osamu Ichii, Takao Irie, Tatsuya Mizoguchi, Akio Shinohara, Hirokazu Kouguchi, Yuji Sunden, Saori Otsuka-Kanazawa, Yaser Hosny Ali Elewa, Chihiro Koshimoto, Ken-Ichi Nagasaki, Yasuhiro Kon
    Histology and Histopathology 33 6 555 - 565 2018年06月01日 [査読無し][通常論文]
     
    Pharyngeal pouches in mammals develop into specific derivatives. If the differentiation of the pharyngeal pouches is anomalous, their remnants can result in cysts, sinuses, and fistulae in the differentiated organs or around the neck. In the present study, we found several pharyngeal pouch remnants, such as cystic structures in thymus and parathyroid gland and fossulae extended from the piriform fossa, in the inbred cotton rats maintained at Hokkaido Institute of Public Health (HIS/Hiph) and University of Miyazaki (HIS/Mz). In HIS/Hiph, the fossulae extended from the apex of the piriform fossa into the thyroid glands and were lined with stratified squamous and cuboidal epithelium. Calcitonin-positive C-cells were present within their epithelium in HIS/Hiph. In contrast, the fossulae of HIS/Mz ran outside the thyroid glands toward the parathyroid glands they were lined with columnar ciliated epithelium and a few goblet cells, but had no C-cells, which was consistent with the cystic structures in the thymus and the parathyroid gland. These results indicated that the fossulae were a remnant of the ultimobranchial body in HIS/Hiph and of the thymopharyngeal duct in HIS/Mz. Thus, the fossulae of the piriform fossa resembled the piriform sinus fistula in human. In conclusion, cotton rats frequently possessed pharyngeal pouch remnants, including the piriform sinus fistula, and therefore, might serve as a novel model to elucidate the mechanisms of pharyngeal pouch development.
  • Ahmed E. Noreldin, Yaser Hosny Ali Elewa, Yasuhiro Kon, Katsuhiko Warita, Yoshinao Z. Hosaka
    Acta Histochemica 120 4 323 - 328 2018年05月01日 [査読無し][通常論文]
     
    Osteoblast activating peptide (OBAP) is a newly discovered peptide detected in the rat stomach, which has a major role in osteogenesis. Recently, we revealed its localization in the parietal cells of the rat stomach. There have been no data regarding OBAP expression in the kidney, despite its role in calcium reabsorption in renal tubules. The current study aimed to inspect the expression of OBAP in the kidney of twelve 10-week-old male C3H/HeNJc1 mice using immunohistochemistry, and immunoelectron microscopic localization. The immunohistochemical investigation revealed an OBAP positive reaction mainly in the medulla, which was stronger than the cortex of the kidney and was concentrated in the distal convoluted tubules (DCT), connecting tubules (CT), and the thick limbs of the loop of Henle (HL). Moreover, we clarified that the OBAP was co-distributed with ghrelin and calbindin (markers of the DCT). Interestingly, immunoelectron microscopy demonstrated that OBAP was concentrated in the mitochondrial inner membrane of the DCT and CT. Based on these results, it was concluded that the mitochondria of the DCT, CT, and HL of the mice kidney generate OBAP. Furthermore, our results suggest that OBAP might have a role in the regulation of calcium reabsorption by the renal tubule however, further investigations are required to clarify this potential role.
  • Osamu Ichii, Teppei Nakamura, Takao Irie, Hirokazu Kouguchi, Kozue Sotozaki, Taro Horino, Yuji Sunden, Yaser Hosny Ali Elewa, Yasuhiro Kon
    Experimental biology and medicine (Maywood, N.J.) 243 5 418 - 427 2018年03月 [査読無し][通常論文]
     
    Cotton rat ( Sigmodon hispidus) is a useful experimental rodent for the study of human infectious diseases. We previously clarified that cotton rats, particularly females, developed chronic kidney disease characterized by cystic lesions, inflammation, and fibrosis. The present study investigated female-associated factors for chronic kidney disease development in cotton rats. Notably, female cotton rats developed separation of the pelvic symphysis and hypertrophy in the vaginal parts of the cervix with age, which strongly associated with pyometra. The development of pyometra closely associated with the deterioration of renal dysfunction or immunological abnormalities was indicated by blood urea nitrogen and serum creatinine or spleen weight and serum albumin/globulin ratio, respectively. These parameters for renal dysfunction and immunological abnormalities were statistically correlated. These phenotypes found in the female reproductive organs were completely inhibited by ovariectomy. Further, the female cotton rats with pyometra tended to show more severe chronic kidney disease phenotypes and immunological abnormalities than those without pyometra; these changes were inhibited in ovariectomized cotton rats. With regard to renal histopathology, cystic lesions, inflammation, and fibrosis were ameliorated by ovariectomy. Notably, the immunostaining intensity of estrogen receptor α and estrogen receptor β were weak in the healthy kidneys, but both estrogen receptors were strongly induced in the renal tubules showing cystic changes. In conclusion, the close correlations among female reproductive organ-associated abnormalities, immunological abnormalities, and renal dysfunction characterize the chronic kidney disease features of female cotton rats. Thus, the cotton rat is a unique rodent model to elucidate the pathological crosstalk between chronic kidney disease and sex-related factors. Impact statement The increasing number of elderly individuals in the overall population has led to a concomitant age-related increase in chronic kidney disease. Moreover, the global prevalence of patients with chronic kidney disease is gradually increasing, which poses a serious public health problem. The limited number of spontaneous chronic kidney disease animal models, which resemble chronic kidney disease pathogenesis in elderly individuals, is a major limitation in the development of experimental and curative medicines for chronic kidney disease. This pathological study clarified that sex-related factors, including hormones, and abnormalities of the female reproductive system, such as pyometra, are closely associated with chronic kidney disease development by using cotton rats ( Sigmodon hispidus). Further, ovariectomy inhibited the phenotypes of the female reproductive system, immunological abnormalities, and chronic kidney disease. Thus, this laboratory rodent serves as a novel and useful spontaneous chronic kidney disease model to elucidate the candidate disease factors and the pathogenesis of chronic kidney disease both in human and experimental medicine.
  • Restricted localization of ultimobranchial body remnants and parafollicular cells in the one-humped camel (Camelus dromedarius).
    Nakamura T, Elewa, YHA, Ichii O, Hosotani M, Ghonimi WAM, Tatsumi O, Nagasaki K, Kon Y
    J Vet Med Sci. 80 9 1368 - 1372 2018年 [査読無し][通常論文]
  • Modified scanning electron microscopy reveals pathological crosstalk between endothelial cells and podocytes in a murine model of membranoproliferative glomerulonephritis
    Masum MdA, Ichii O, Elewa YHA, Nakamura T, Otani Y, Hosotani M, Kon Y
    Scientific Reports 8 10276 2018年 [査読無し][通常論文]
  • Ovarian mast cells migrate toward ovary- fimbria connection in neonatal MRL/MpJ mice.
    Nakamura T, Chihara M, Ichii O, Otsuka-Kanazawa S, Nagasaki K, Elewa YHA, Tatsumi O, Kon Y
    PLOS ONE. 13 4 2018年 [査読無し][通常論文]
  • Histopathological correlations between mediastinal fat-associated lymphoid clusters and development of lung inflammation and fibrosis following bleomycin administration in mice.
    Elewa YHA, Ichii O, Takada K, Nakamura T, Masum MdA, Kon Y
    Frontier in Immunology. 9 271 2018年 [査読無し][通常論文]
  • Protective Effects of Miswak (Salvadora persica) against Experimentally Induced Gastric Ulcers in Rats. Oxidative Medicine and Cellular Longevity
    エレワ ヤセル
    Oxidative Medicine and Cellular Longevity. 2018 6703296 2018年 [査読無し][通常論文]
  • Samah R. Khalil, Walaa M. Elhady, Yaser H.A. Elewa, Noura E. Abd El-Hameed, Sozan A. Ali
    Biomedicine and Pharmacotherapy 97 1259 - 1268 2018年01月01日 [査読無し][通常論文]
     
    Environmental pollutants, particularly metallic elements, mobilized and released into the environment, eventually accumulate in the food chain and thus pose a serious threat to human and animal health. In the present study, the role of Arthrospira (Spirulina platensis SP) as a protector against oxidative stress-mediated liver damage induced by an exposure to lead acetate (LA as a metallic pollutant) was assessed. To achieve this aim, rats were orally administered with 300 mg/kg bw SP for 15 days, before and concurrently with an intraperitoneal injection of 50 mg/kg bw LA (6 injections throughout 15 days). As a result, co-administration of SP with LA reduced the amount of lead that accumulated in both blood and liver tissue of the exposed rats and minimized the increased levels of lipid peroxidation, protein oxidation, DNA oxidative damage, and liver enzyme endpoints. In addition, because of SP administration, the levels of depleted biomarkers of antioxidant status and total antioxidant capacity in LA-exposed rats improved. Moreover, SP protected the liver tissue against the changes caused by LA exposure and also decreased the reactivity of HSP70 in the cytoplasm of hepatocytes. Collectively, our data suggest that SP has a potential use as a food supplement in the regions highly polluted with heavy metals such as lead.
  • Ichii O, Kimura J, Okamura T, Horino T, Nakamura T, Sasaki H, Elewa YHA, Kon Y
    Frontiers in Immunology 8 1346 2017年10月 [査読無し][通常論文]
     
    IL-36 alpha, a member of the IL-1 family, is a crucial mediator of inflammatory responses. We previously found that IL-36 alpha was overexpressed in injured distal tubules (DTs); however, its pathological function remains unclear. Herein, unilateral ureter obstruction (UUO) or folic acid (FA) injection was performed in mouse kidneys to assess the role of IL-36 alpha in kidney injury. IL-36 alpha mRNA and protein expression significantly increased in the kidneys within 24 h after UUO. IL-36 alpha localized to dilated DTs. IL-36 alpha expression significantly correlated with the progression of tubulointerstitial cell infiltration and tubular epithelium cell death in UUO kidneys and with renal dysfunction in FA-induced acute kidney injury mice. At 24 h after UUO, IL-36 alpha(+) DT epithelial cells showed loose intercellular digitations. IL-1RL2, an IL-36 alpha receptor protein, localized to podocytes, proximal tubules, and DTs in the healthy kidney. IL-1RL2 was expressed in interstitial cells and platelets or extended primary cilia of DT epithelial cells in UUO kidneys. IL-36 alpha stimulation promoted the production of IL-6 and Prss35, an inflammatory cytokine and collagen remodeling-associated enzyme, respectively, in cultured NIH3T3 fibroblasts. UUO-treated IL-36 alpha-knockout (KO) mice showed milder kidney injury features than wild-type (WT) mice did. In UUO kidneys from IL-36 alpha-KO mice, the expression of genes associated with inflammatory response and sensory perception was significantly different from that in WT mice. Altogether, our data indicate an association between intrarenal IL-36 alpha overexpression and the progression of tubulointerstitial inflammations and morpho-functional alterations of DT epithelial cells. IL-36 alpha may be a novel kidney injury marker useful for evaluating DT damages.
  • Teppei Nakamura, Naoya Karakida, Ai Dantsuka, Osamu Ichii, Yaser Hosny Ali Elewa, Yasuhiro Kon, Ken-ichi Nagasaki, Hideki Hattori, Tomoji Yoshiyasu
    JOURNAL OF VETERINARY MEDICAL SCIENCE 79 7 1230 - 1235 2017年07月 [査読有り][通常論文]
     
    Syrian golden hamsters (Mesocricetus auratus) are useful laboratory rodents for studying human infectious diseases, metabolic diseases and cancer. In other rodents, such as mice and rats, a mixture of medetomidine, midazolam and butorphanol functions as a useful anesthetic, although it alters some blood biochemical parameters. In this study, we examined the effects of this mixture on anesthesia and blood biochemical parameters, and the action of atipamezole, a medetomidine antagonist, in hamsters. Intramuscular injection of a mixture of medetomidine, midazolam and butorphanol at doses of 0.15, 2.0 and 2.5 mg/kg, respectively, had a short induction time (within 5 min) and produced an anesthetic duration of approximately 100 min in hamsters. We also demonstrated that 0.15 mg/kg of atipamezole, corresponding to the same dose as medetomidine, made hamsters recover quickly from anesthesia. The anesthetic agent markedly altered metabolic parameters, such as plasma glucose and insulin; however, 0.15 mg/kg of atipamezole returned these levels to normal range within approximately 10 min after the injection. The anesthetic also slightly altered mineral levels, such as plasma inorganic phosphorus, calcium and sodium; the latter two were also improved by atipamezole. Our results indicated that the mixture of medetomidine, midazolam, and butorphanol at doses of 0.15, 2.0 and 2.5 mg/kg, respectively, functioned as an effective anesthetic, and atipamezole was useful for antagonizing both anesthesia and biochemical alteration in hamsters.
  • Osamu Ichii, Masataka Chihara, Shin-Hyo Lee, Teppei Nakamura, Saori Otsuka-Kanazawa, Taro Horino, Yaser Hosny Ali Elewa, Yasuhiro Kon
    AUTOIMMUNITY 50 2 114 - 124 2017年03月 [査読有り][通常論文]
     
    Inbred MRL/MpJ mice show several unique phenotypes in tissue regeneration processes and the urogenital and immune systems. Clarifying the genetic and molecular bases of these phenotypes requires the analysis of their genetic susceptibility locus. Herein, hydronephrosis development was incidentally observed in MRL/MpJ-derived chromosome 11 (D11Mit21-212)-carrying C57BL/6N-based congenic mice, which developed bilateral or unilateral hydronephrosis in both males and females with 23.5% and 12.5% prevalence, respectively. Histopathologically, papillary malformations of the transitional epithelium in the pelvic-ureteric junction seemed to constrict the ureter luminal entrance. Characteristically, eosinophilic crystals were observed in the lumen of diseased ureters. These ureters were surrounded by infiltrating cells mainly composed of numerous CD3(+)T-cells and B220(+)B-cells. Furthermore, several Iba-1(+)macrophages, Gr-1(+)granulocytes, mast cells and chitinase 3-like 3/Ym1 (an important inflammatory lectin)-positive cells were detected. Eosinophils also accumulated to these lesions in diseased ureters. Some B6.MRL-(D11Mit21-D11Mit212) mice had duplicated ureters. We determined>100 single nucleotide variants between C57BL/6N- and MRL/MpJ-type chromosome 11 congenic regions, which were associated with nonsynonymous substitution, frameshift or stopgain of coding proteins. In conclusion, B6.MRL-(D11Mit21-D11Mit212) mice spontaneously developed hydronephrosis due to obstructive uropathy with inflammation. Thus, this mouse line would be useful for molecular pathological analysis of obstructive uropathy in experimental medicine.
  • Local CD34-positive capillaries decrease in mouse models of kidney disease associating with the severity of glomerular and tubulointerstitial lesions
    Masum MdA, Ichii O, Elewa, YHA, Nakamura T, Kon Y
    BMC Nephrol. 18 280 2017年 [査読無し][通常論文]
  • Hosotani M, Ichii O, Nakamura T, Otsuka-Kanazawa S, Elewa YHA, Kon Y
    Lupus 961203317711772  2017年01月 [査読有り][通常論文]
  • Teppei Ikeda, Osamu Ichii, Saori Otsuka-Kanazawa, Teppei Nakamura, Yaser Hosny Ali Elewa, Yasuhiro Kon
    JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY 37 4-5 153 - 164 2016年10月 [査読有り][通常論文]
     
    Skeletal muscle myofibers constantly undergo degeneration and regeneration. Histopathological features of 6 skeletal muscles (cranial tibial [CT], gastrocnemius, quadriceps femoris, triceps brachii [TB], lumbar longissimus muscles, and costal part of the diaphragm [CPD]) were compared using C57BL/10ScSn-Dmd (mdx) (mdx) mice, a model for muscular dystrophy versus control, C57BL/10 mice. Body weight and skeletal muscle mass were lower in mdx mice than the control at 4 weeks of age; these results were similar at 6-30 weeks. Additionally, muscular lesions were observed in all examined skeletal muscles in mdx mice after 4 weeks, but none were noted in the controls. Immunohistochemical staining revealed numerous paired box 7-positive satellite cells surrounding the embryonic myosin heavy chain-positive regenerating myofibers, while the number of the former and staining intensity of the latter decreased as myofiber regeneration progressed. Persistent muscular lesions were observed in skeletal muscles of mdx mice between 4 and 14 weeks of age, and normal myofibers decreased with age. Number of muscular lesions was lowest in CPD at all ages examined, while the ratio of normal myofibers was lowest in TB at 6 weeks. In CT, TB, and CPD, Iba1-positive macrophages, the main inflammatory cells in skeletal muscle lesions, showed a significant positive correlation with the appearance of regenerating myofibers. Additionally, B220-positive B-cells showed positive and negative correlation with regenerating and regenerated myofibers, respectively. Our data suggest that degenerative and regenerative features of myofibers differ among skeletal muscles and that inflammatory cells are strongly associated with regenerative features of myofibers in mdx mice.
  • Truc Quynh Thai, Huy Bang Nguyen, Sei Saitoh, Bao Wu, Yurika Saitoh, Satoshi Shimo, Yaser Hosny Ali Elewa, Osamu Ichii, Yasuhiro Kon, Takashi Takaki, Kensuke Joh, Nobuhiko Ohno
    MEDICAL MOLECULAR MORPHOLOGY 49 3 154 - 162 2016年09月 [査読有り][通常論文]
     
    Serial block-face imaging using scanning electron microscopy enables rapid observations of three-dimensional ultrastructures in a large volume of biological specimens. However, such imaging usually requires days for sample preparation to reduce charging and increase image contrast. In this study, we report a rapid procedure to acquire serial electron microscopic images within 1 day for three-dimensional analyses of subcellular ultrastructures. This procedure is based on serial block-face with two major modifications, including a new sample treatment device and direct polymerization on the rivets, to reduce the time and workload needed. The modified procedure without uranyl acetate can produce tens of embedded samples observable under serial block-face scanning electron microscopy within 1 day. The serial images obtained are similar to the block-face images acquired by common procedures, and are applicable to three-dimensional reconstructions at a subcellular resolution. Using this approach, regional immune deposits and the double contour or heterogeneous thinning of basement membranes were observed in the glomerular capillary loops of an autoimmune nephropathy model. These modifications provide options to improve the throughput of three-dimensional electron microscopic examinations, and will ultimately be beneficial for the wider application of volume imaging in life science and clinical medicine.
  • Osamu Ichii, Teppei Nakamura, Takao Irie, Hirokazu Kouguchi, Daisuke Nakamura, Saori Nakamura, Shinobu Sato, Keisuke Yokoyama, Taro Horino, Yuji Sunden, Yaser Hosny Ali Elewa, Yasuhiro Kon
    HISTOCHEMISTRY AND CELL BIOLOGY 146 3 351 - 362 2016年09月 [査読有り][通常論文]
     
    The cotton rat (Sigmodon hispidus) is a laboratory rodent that has been used for studies on human infectious diseases. In the present study, we observed that female cotton rats, not the male cotton rats, developed chronic anemia characterized by reduced red blood cell, hemoglobin, and hematocrit levels from 5 to 9 months of age without any changes in the mean corpuscular hemoglobin and volume levels. In peripheral blood, the reticulocyte count did not increase in response to anemia in female cotton rats, and no extramedullary hematopoiesis was observed in the liver or spleen. Further, the serum levels of urea nitrogen and creatinine increased from 5 to 9 months of age in female cotton rats compared to male cotton rats, and these increases became more prominent from 10 months of age onward, indicating chronic kidney disease. Histopathologically, female cotton rats manifested tubulointerstitial lesions characterized by the infiltration of mononuclear cells, including plasma cells and CD3(+) T-cells, as well as the dilation of calbindin-D28k(+) distal tubules from 5 to 9 months of age. The severity of these lesions progressed from 10 months of age onward, and renal fibrotic features and numerous tubular cysts appeared without any obvious glomerular lesions. A significant decrease in the erythropoietin protein levels was observed in the kidney of aged female cotton rats, and significant correlations were detected between anemia and tubulointerstitial damage. These results suggest that aged female cotton rats chronically develop renal anemia, and this rodent may serve as a novel model to elucidate its pathogenesis.
  • Sawa Onouchi, Osamu Ichii, Teppei Nakamura, Yaser Hosny Ali Elewa, Yasuhiro Kon
    CELL AND TISSUE RESEARCH 365 2 367 - 379 2016年08月 [査読有り][通常論文]
     
    Although gut flexures characterize gut morphology, the mechanisms underlying flexure formation remain obscure. Previously, we analyzed the mouse duodenojejunal flexure (DJF) as a model for its formation and reported asymmetric morphologies between the inner and outer bending sides of the fetal mouse DJF, implying their contribution to DJF formation. We now present the extracellular matrix (ECM) as an important factor for gut morphogenesis. We investigate ECM distribution during mouse DJF formation by histological techniques. In the intercellular space of the gut wall, high Alcian-Blue positivity for proteoglycans shifted from the outer to the inner side of the gut wall during DJF formation. Immunopositivity for fibronectin, collagen I, or pan-tenascin was higher at the inner than at the outer side. Collagen IV and laminins localized to the epithelial basement membrane. Beneath the mesothelium at the pre-formation stage, collagen IV and laminin immunopositivity showed inverse results, corresponding to the different cellular characteristics at this site. At the post-formation stage, however, laminin positivity beneath the mesothelium was the reverse of that observed during the pre-formation stage. High immunopositivity for collagen IV and laminins at the inner gut wall mesenchyme of the post-formation DJF implied a different blood vessel distribution. We conclude that ECM distribution changes spatiotemporally during mouse DJF formation, indicating ECM association with the establishment of asymmetric morphologies during this process.
  • Daichi Shiozuru, Osamu Ichii, Junpei Kimura, Teppei Nakamura, Yaser Hosny Ali Elewa, Saori Otsuka-Kanazawa, Yasuhiro Kon
    HISTOLOGY AND HISTOPATHOLOGY 31 2 189 - 204 2016年02月 [査読有り][通常論文]
     
    Clarification of the renal repair process is crucial for developing novel therapeutic strategies for kidney injury. MRL/MpJ mice have a unique repair process characterized by low scar formation. The pathological features of experimentally injured MRL/MpJ and C57BL/6 mouse kidneys were compared to examine the renal repair process. The dilation and atrophy of renal tubules were observed in folic acid (FA)-induced acute kidney injury (AKI) in both strains, and the histopathological injury scores and number of interleukin (IL)-1F6-positive damaged distal tubules and kidney injury molecule 1 (KIM-1)-positive damaged proximal tubules drastically increased 1 day after AKI induction. However, KIM-1-positive tubules and the elevation of serum renal function markers were significantly fewer and lower, respectively, in MRL/MpJ mice at days 2 and 7 after AKI. After traumatic kidney injury (TKI) via needle puncture, severe tubular necrotic lesions in the punctured area and fibrosis progressed in both strains. Indices for fibrosis such as aniline blue-positive area, number of alpha smooth muscle actin-positive myofibroblasts, and messenger RNA expression levels of Tgfb1 and Mmp2 indicated lower fibrotic activity in MRL/MpJ kidneys. Characteristically, only MRL/MpJ kidneys manifested remarkable calcification around the punctured area beginning 7 days after TKI. The pathological features of injured MRL/MpJ and C57BL/6 kidneys differed, especially those of kidneys with mild proximal tubular injuries after FA-induced AKI. Lower fibrotic activity and increased calcification after TKI were observed in MRL/MpJ kidneys. These findings clarified the unique pathological characteristics of MRL/MpJ mouse kidneys and contribute to understanding of the renal repair process after kidney injury.
  • Yaser Hosny Ali Elewa, Osamu Ichii, Yasuhiro Kon
    IMMUNOLOGY 147 1 30 - 40 2016年01月 [査読有り][通常論文]
     
    We previously discovered mediastinal fat-associated lymphoid clusters (MFALCs) as novel lymphoid clusters associated with mediastinal fat tissue in healthy mice. However, no data about their morphology in immune-associated disease conditions, and their relationship with lung infiltration, is available to date. In the present study, we compared the morphological features of MFALCs in 4-month-old male murine autoimmune disease models (MRL/MpJ-lpr mice and BXSB/MpJ-Yaa mice) with those of the corresponding control strains (MRL/MpJ and BXSB/MpJ, respectively). In addition, we analysed their correlation with lung infiltration. Furthermore, immunohistochemistry for CD3, B220, Iba1, Gr1 and BrdU was performed to detect T cells and B cells, macrophages, granulocytes and proliferating cells, respectively. The spleen weight to body weight ratios and anti-double-stranded DNA autoantibody titres were found to be significantly higher in the autoimmune models than in the control strains. Furthermore, the autoimmune model presented prominent MFALCs, with a significantly greater ratio of lymphoid cluster area to total mediastinal fat tissue area, and more apparent diffused cellular infiltration into the lung lobes than the other studied strains. Higher numbers of T and B cells, macrophages and proliferating cells, but fewer granulocytes, were observed in the autoimmune models than in the control strains. Interestingly, a significant positive Pearson's correlation between the size of the MFALCs and the density of CD3-, B220- and Iba1-positive cells in the lung was observed. Therefore, our data suggest a potentially important role for MFALCs in the progression of lung disease. However, further investigation is required to clarify the pathological role of MFALCs in lung disease, especially in inflammatory disorders.
  • M. Chihara, T. Nakamura, S. Otsuka-Kanazawa, O. Ichii, Y. H. A. Elewa, Y. Kon
    ANDROLOGY 3 5 991 - 999 2015年09月 [査読有り][通常論文]
     
    MRL/MpJ mice possess highly heat-shock-resistant spermatocytes (HRS) in comparison with C57BL/6 mice. This resistance depends on the MRL/MpJ-type loci at the 81 cM region of Chromosome (Chr) 1 and the 40 cM region of Chr 11. To evaluate the functions of these loci in detail, we examined the histopathological changes resulting from experimental cryptorchidism or transient scrotal heat stress (SHS) in the testes of C57BL/6-based congenic strains (B6.MRLc1, B6.MRLc11, and B6.MRLc1c11) carrying the MRL/MpJ-derived loci responsible for HRS. Among cryptorchid testes from congenic strains, those in B6.MRLc1c11 mice showed the highest heat resistance, indicating that the genetic interactions between MRL/MpJ-derived HRS loci on Chrs 1 and 11 may be important for maintaining spermatogenesis under continuous testicular hyperthermia. In contrast, immediately after SHS induction, germ cell loss via apoptosis was inhibited in B6.MRLc11 and B6.MRLc1c11 mice, similar to that in MRL/MpJ mice. However, this HRS phenotype was not observed in C57BL/6 or B6.MRLc1 mice after SHS induction. Furthermore, testicular calcification owing to long-term damage by SHS induction was inhibited in all congenic strains in comparison with that in C57BL/6 mice, indicating that each MRL/MpJ-derived locus on Chrs 1 and 11 acted independently to facilitate the recovery of heat-induced testicular damage by inhibiting calcification. B6.MRLc11 and B6.MRLc1c11 mice showed greater recovery in spermatogenesis than B6.MRLc1 mice 60 days after SHS induction. Therefore, the MRL/MpJ-derived HRS locus on Chr 11 might play an important role in recovery from heat stress damage. On the basis of these results, we concluded that MRL/MpJ-derived loci on Chrs 1 and 11 cooperatively or independently regulate testicular heat sensitivity depending on the various heat stresses.
  • Amany Abdel-Rahman Mohamed, Azza A. A. Galal, Yaser H. A. Elewa
    ACTA HISTOCHEMICA 117 7 649 - 658 2015年 [査読有り][通常論文]
     
    This study is aimed to evaluate the possible neurotoxic effect of tartrazine (T), an extensively used synthetic azo dye, as well as to determine the potential modulatory role of cod liver oil (CLO) or royal jelly (RJ) against such effects. For this purpose, thirty-six male rat pups were allocated into six groups. The 1st group received distilled water (control group), the 2nd group was given 300 mg RJ/kg bw (RJ group), the 3rd group was given 0.4 ml CLO/kg bw (CLO group), the 4th was given 500 mg T/kg bw (T group). The 5th group was given T concurrently with RJ (TRJ group) and the 6th group was given T concurrently with CLO (TCLO group), at the same doses as the former groups. All treatments were given orally for 30 consecutive days. The concentrations of different brain neurotransmitters, gamma amino butyric acid (GABA), dopamine (DA) and serotonin (5HT) as well as the antioxidant and oxidative stress biomarkers were measured in the brain homogenates. An immunohistochemical staining of the cerebral cortex was applied with the anti-ssDNA antibody (an apoptotic cell marker) to reveal the changes in brain structure. The T group revealed a significant decrease in the concentration of the brain neurotransmitters, a sharp shortage in the level of antioxidant biomarkers (super oxide dismutase, catalase and the reduced glutathione), a marked increase in malondialdehyde levels, and numerous apoptotic cells in the brain cortex compared with the other groups. Interestingly, all the previously mentioned parameters were almost retrieved in both the TRJ and TCLO groups compared to the T group. These results conclusively demonstrate that RJ and CLO administration provides sufficient protection against the ruinous effects of T on rat pups brain tissue function and structure. (C) 2015 Elsevier GmbH. All rights reserved.
  • Yaser Hosny Ali Elewa, Osamu Ichii, Saori Otsuka, Yoshiharu Hashimoto, Yasuhiro Kon
    CELL AND TISSUE RESEARCH 357 3 731 - 741 2014年09月 [査読有り][通常論文]
     
    The association between adipose tissue and immunity has been established and fat-associated lymphoid clusters (FALCs) are considered as a source of immune cells. We discovered lymphoid clusters (LCs) in mouse mediastinal fat tissues (MFTs). In Th1-biased C57BL/6N (B6), Th2-biased DBA/2Cr (DBA) and autoimmune-prone MRL/MpJ (MRL) mice strains, LCs without a fibrous capsule and germinal center were observed in white-colored MFTs extending from the diaphragm to the heart. The number and size of the LCs were larger in 12-month-old mice than in 3-month-old mice in all of the examined strains. Moreover, B6 had an especially large number of LCs compared with DBA and MRL. The immune cells in the LCs consisted of mainly T-cells and some B-cells. The majority of T-cells were CD4+ helper T (Th) cells, rather than CD8+ cytotoxic T-cells and no obvious immune cell population difference was present among the strains. Furthermore, high endothelial venules and lymphatic vessels in the LCs were better developed in B6 mice than in the other strains. Interestingly, some CD133+ hematopoietic progenitor cells and some c-Kit+/CD127+ natural helper cells were detected in the LCs. BrdU+ proliferating cells were more abundant in the LCs of B6 mice than in the LCs of the other strains and the number of BrdU+ cells increased with age. This is the first report of LCs in mouse MFTs. We suggest that the mouse genetic background affects LC size and number. We term the LCs "mediastinal fat-associated lymphoid clusters". These clusters can be considered as niches for Th cell production.
  • Y. H. A. Elewa, O. Ichii, S. Otsuka, Y. Hashimoto, Y. Kon
    ANATOMIA HISTOLOGIA EMBRYOLOGIA 43 4 265 - 272 2014年08月 [査読有り][通常論文]
     
    Previously, the structure of the adult goat parotid salivary glands (PGs) was studied. However, little information was elucidated of the juvenile ones. This study aimed to clarify the correlations between the structure of goats' PGs and the nature of food intake among milk-suckling kids (MSKs) and diet-fed goats (DFGs). The secretory endpieces of the goats' PGs are of the pure serous type. The serous cells in MSKs showed apical accumulation of numerous secretory granules (SGs) of smaller size and of more intense positive periodic acid-Schiff reaction. Ultrastructurally, most of the SGs in the DFGs contained peripherally located inclusions that showed dense reaction products for acid phosphatase. In MSKs, the PGs showed less-developed basal infoldings, sparseness of the inter-cellular inter-digitations, fewer inter-cellular canaliculi and microvilli and also less-developed myoepithelial cells with fewer and shorter cytoplasmic processes. In conclusion, the less-developed membrane specializations and myoepithelial cells, as well as the accumulated SGs in the PGs of MSKs, suggest that it secretes less saliva with a little secretory activity than that of DFGs, which may be correlated with the reduced masticatory activity.
  • Lee SH, Ichii O, Otsuka S, Elewa YH, Namiki Y, Hashimoto Y, Kon Y
    Journal of anatomy 219 6 743 - 755 2011年12月 [査読有り][通常論文]
     
    MRL/MpJ (MRL) mice, commonly used as a model for autoimmune disease, have a high frequency of ovarian cysts originating from the rete ovarii. In the present study, to clarify how the rete ovarii, which are remnants of mesonephric tubules during embryogenesis, progress to cystic formation with aging, the morphology of MRL rete ovarii was analyzed and compared with that of normal C57BL/6N (B6) mice. In B6 mice, the rete ovarii consisted of a series of tubules, including the extraovarian rete (ER), the connecting rete (CR), and the intraovarian rete (IR), based on their location. Whereas the ER of B6 mice was composed of highly convoluted tubules lined by both ciliated and non-ciliated epithelia, the tubules in the CR and IR had only non-ciliated cells. In MRL mice, dilations of the rete ovarii initiated from the IR rather than the ER or CR. Although the histological types of cells lining the lumen of the rete ovarii were the same as those in B6 mice, the ER in MRL mice showed a variety in morphology. In particular, the connections between the ER and ovary tended to disappear with increasing age and the development of ovarian cysts. Furthermore, the epithelium lining the large ovarian cysts in MRL mice had ciliated cells forming the cluster. On the basis of these findings, it is suggested that cystic changes of the rete ovarii in MRL mice are caused by the dilations of the IR with invasion of the ER and CR into the ovarian medulla. These data provide new pathological mechanisms for ovarian cyst formation.
  • Yaser Hosny Elewa, Mohammad Hafez Bareedy, Ahmed Awad Abu Al Atta, Osamu Ichii, Saori Otsuka, Tomonori Kanazawa, Shin-Hyo Lee, Yoshiharu Hashimoto, Yasuhiro Kon
    VETERINARY RESEARCH COMMUNICATIONS 34 6 557 - 567 2010年08月 [査読有り][通常論文]
     
    Previously, the distribution of myoepithelial cells (mecs) in the salivary glands was studied by both immunohistochemistry, and transmission electron microscopy; however, little was elucidated concerning their morphological features, especially in goats. This study was performed to investigate the correlation between the cytoarchitecture of the mecs in goat major salivary glands (parotid, mandibular, and sublingual glands) and the nature of the saliva secretion. The cytoarchitectural features of the mecs were examined by scanning and transmission electron microscopy as well as immunohistochemically. The secretory endpieces in the parotid gland are of the pure serous type, but in both the mandibular and sublingual glands they are of the mixed type. In all studied glands, the intercalated ducts were covered by mecs which, unlike the large stellate cells that surrounded the secretory endpieces, were spindle-shaped with few cytoplasmic processes. Interestingly, the mecs were found to bulge on the basal surfaces of the serous acini and intercalated ducts in all glands and to be in close contact to the seromucous tubules surface in the mandibular and sublingual glands forming a continuous network around it. In conclusion, the differences in the degree of development of the mecs as well as the number of their cytoplasmic processes may be correlated with the nature of the secretion and the number of the secretory granules. Thus these observations may have some relevance in the diagnosis of atrophy and pathogenic conditions of these glands.
  • Yaser Hosny Elewa, Mohammad Hafez Bareedy, Ahmed Awad Abuel-Atta, Osamu Ichii, Saori Otsuka, Tomonori Kanazawa, Shinhyo Lee, Yoshiharu Hashimoto, Yasuhiro Kon
    JAPANESE JOURNAL OF VETERINARY RESEARCH 58 2 121 - 135 2010年08月 [査読有り][通常論文]
     
    The structural characteristics of the parotid glands in small ruminants (goat, sheep) were observed and compared to those of a major laboratory animal, the mouse. Their parotid glands consist of the purely serous type. Ultrastructurally, the serous acini of goats and sheep were characterized by the presence of well-developed basolateral expansions of folds, which are characteristics of electrolyte- and water-transporting epithelium. Moreover in ruminants, unlike the mouse, the presence of numerous intercellular canaliculi as well as microvilli projecting into both the intercellular canaliculi and the lumina of the serous acini provided a large surface area for osmotic equilibrium and isotonic saliva secretion. Most of the secretory granules in goats and sheep contained peripherally located inclusions that showed dense reaction products for acid phosphatase. This indicates that most of the secretory granules undergo lysosomal degradation rather than secretion. An apocrine mode of secretion of some secretory granules was occasionally observed in some acini of goats and sheep but only exocytotic features were observed in mice. In the goat, the serous acini showed three morphologically different types, which might be an indication of different activity phases. Furthermore, alpha-smooth muscle actin-, and vimentin-positive myoepithelial cells were observed only around the serous acini and the intercalated ducts. From these findings, we consider that the structural characteristics of ruminant parotid glands might reflect their physiological role in the copious isotonic saliva secretion with a low protein concentration.
  • Sameh Magdeldin, Yaser Elewa, Takako Ikeda, Junko Ikei, Ying Zhang, Bo Xu, Masaaki Nameta, Hidehiko Fujinaka, Yutaka Yoshida, Eishin Yaoita, Tadashi Yamamoto
    GENERAL PHYSIOLOGY AND BIOPHYSICS 28 3 266 - 275 2009年09月 [査読無し][通常論文]
     
    In order to investigate the effects of dietary supplementation rich in omega 3 and omega 6 fatty acids, we set up an experiment of twenty four C57BL/6J male mice segregated into 3 groups: normal diet (ND), omega 3 polyunsaturated fatty acid (n-3 PUFA,) and omega 6 (n-6 PUFA). At the end of the experiment that lasted for I month, food consumption of ND and n-3 PUFA were similar while it decreased in n-6 PUFA group. Total cholesterol, triglycerides, free fatty acids, and phospholipids profiles were increased in n-6 PUFA. LDL decreased in n-3 PUFA while increased in n-6 PUFA fed mice comparing to control group. On the other hand, there was no difference between treatments in HDL and glucose levels. Expression of leptin (ob) gene transcripts in epididymal fat were significantly elevated in n-6 PUFA mice compared to ND and n-3 PUFA groups while hypothalamic ob receptor A (obRa) mRNA did not changed in response to diet regimes. Transmission and scanning electron microscopy showed different degrees in fatty changes in the liver of both PUFA groups including lipid droplet infiltration and Ito cells with over accumulated lipids. In conclusion, under PUFA dietary supplementation, the hyperlipidemic status and elevated ob expression of n-6 PUFA but not n-3 PUFA fed mice suggests altered lipid metabolism between PUFA groups and/or different endocrine involvement. Moreover, the coincidently structural changes observed in liver of this group direct us to call for further studies to investigate the anti-obesity effect and safety of these PUFA under high supplementation condition.

その他活動・業績

  • “Overexpression of Toll-like receptor 9 correlates with podocyte injury found in murine autoimmune glomerulonephritis.
    エレワ ヤセル The 6th Sapporo Summer Seminar for One Health (SASSOH). September 20-21, 2018. Sapporo, Japan. 6th 2018年09月 [査読無し][通常論文]
  • “Lymphoid tissues appearing in the urinary tract of animals (Urinary tract-associated lymphoid tissue, UTALT)”.
    O. Ichii, T. Nakamura, R. Ono, T. Horino, Y. H. A. Elewa, Y. Kon The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan. 161th 2018年09月 [査読無し][通常論文]
  • “Morphological characteristics of the dromedary thyroid gland - and distribution of C cells”.
    T. Nakamura, O. Ichii, Y. H. A. Elewa, W. A. M. Ghonimi, K. Nagasaki, Y. Kon The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan. 161th 2018年09月 [査読無し][通常論文]
  • “New findings of kidney disease - Kidney lesions found in Casp3 deficient mice”.
    T. Suzuki, O. Ichii, T. Nakamura, Y. H. A. Elewa, Y. Kon The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan. 161th 2018年09月 [査読無し][通常論文]
  • “Morphological and functional changes observed in bone of autoimmune disease model”.
    T. Namba, O. Ichii, Y. H. A. Elewa, T. Nakamura, J. Kimura, Y. Otani, Md. A. Masum, Y. Kon The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan. 161th 2018年09月 [査読無し][通常論文]
  • Investigation for meiotic apoptosis of spermatocyte in autoimmune disease model mouse.
    エレワ ヤセル The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan. 161th 2018年09月 [査読無し][通常論文]
  • Effect of systemic autoimmune abnormality in MRL/MpJ-Faslpr/lpr mice on the ciliary function.
    M. Hosotani, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan 161th 2018年09月 [査読無し][通常論文]
  • “Mediastinal fat-associated lymphoid cluster and lung asthma development in papain induced lung asthma mice model
    Y. H. A. Elewa, T. Nakamura, O. Ichii, Y. Kon The 64th annual meeting of the Japanese the Anatomical Society (Oral Presentation). Aomori, Japan. 64th 2018年09月 [査読無し][通常論文]
  • “Glomerular lesions seen in male cotton rats (Sigmodon hispidus)”
    T. Nakamura, O. Ichii, T. Irie, H. Kouguchi, Sunden, Masum, M. A., Y, Y. H. A. Elewa, Y. Kon The 11th Annual Meeting of the Japanese Society of Veterinary Renal Urology. 11th 2018年08月 [査読無し][通常論文]
  • Local CD34-positive capillary decreased with the progression of lesion in respective area of kidney in mice.
    Md. A. Masum, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 17th International Conference on Nephrology and Urology. London, UK. 13th March 2018. 17th 2018年03月 [査読無し][通常論文]
  • “Dual effects of bleomycin on the intra-thoracic immune hemostasis and lung injury in autoimmune disease model mice.”
    Y. H. A. Elewa, O. Ichii, S, Y. Kon The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA). Kuching, Sarawak, Malaysia. 6th P-18 2017年12月 [査読無し][通常論文]
  • BXSB/MpJ-Yaa MICE SHOW THE INCREASE OF APOPTOTIC SPERMATOCYTES IN STAGE Ⅻ SEMINIFEROUS TUBULES.
    Y. Otani, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA). Kuching, Sarawak, Malaysia. October 12-13, 2017. 6th 2017年10月 [査読無し][通常論文]
  • ABNOLMALITY OF OVULATION AND OOCYTE-PICK-UP IN MRL/MpJ-Faslpr/lpr MICE.
    Hosotani, O. Ichii, Y. H. A. Elewa, T. Nakamura, S. Otsuka-Kanazawa, Y. Kon The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia 6th 2017年10月 [査読無し][通常論文]
  • IL-1F6/IL-36α SIGNALING REGULATES RENAL INFLAMMATION.
    O. Ichii, J. Kimura, T. Okamura, T. Horino, T. Nakamura, H. Sasaki, H. A. Elewa, Y. Kon The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia 6th 2017年10月 [査読無し][通常論文]
  • FAT ACCUMULATION CORRELATES WITH DIABETES MELLITUS IN MALE COTTON RATS (SIGMODON HISPIDUS).
    T. Nakamura, O. Ichii, T. Irie, H. Kouguchi, Y. Sunden, K.-I. Nagasaki, Y. H. A. Elewa, Y. Kon The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia 2017年10月 [査読無し][通常論文]
  • “Mechanism and abnormality of ovulation and picking up of mammalian oocytes-Immune factors affecting the molecular morphology of the oviduct.”
    M. Hosotani, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 5th Sapporo Summer Seminar for One Health (SASSOH). September 20-21, 2017. Sapporo, Japan. 5th 2017年09月 [査読無し][通常論文]
  • “Loss of endothelial fenestration correlates with podocyte injury in the mouse model of membranoproliferative glomerulonephritis.”
    Md. A. Masum, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 5th Sapporo Summer Seminar for One Health (SASSOH). September 20-21, 2017. Sapporo, Japan. 5th 2017年09月 [査読無し][通常論文]
  • “The unique phenotype in ovulation and corpus luteum formation in MRL/MpJ mouse.”
    M. Hosotani, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 160th Meeting of the Japanese Society of Veterinary Sciences, September 13-16, 2017. Kagoshima, Japan. 160th 2017年09月 [査読無し][通常論文]
  • “Pathological correlations between endothelial cells and podocytes in autoimmune-related injury of the mouse glomerulus.”
    Md. A. Masum, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 160th Meeting of the Japanese Society of Veterinary Sciences, September 13-16, 2017. Kagoshima, Japan. 160th 2017年09月 [査読無し][通常論文]
  • Histopathological features observed in the testis of autoimmune-prone BXSB/MpJ-Yaa mice.
    Y. Otani, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 5th Sapporo Summer Seminar for One Health. 5th 2017年09月 [査読無し][通常論文]
  • Apoptosis of spermatocytes seen in BXSB / MpJ-Yaa mice - association with autoimmune abnormality.
    Y. Otani, O. Ichii, Y. H. A. Elewa, T. Nakamura, Y. Kon The 160th Meeting of the Japanese Society of Veterinary Sciences. 160th 2017年09月 [査読無し][通常論文]
  • “Dual effects of bleomycin on the intra-thoracic immune hemostasis and lung injury in autoimmune disease model mice”.
    Y. H. A. Elewa, T. Nakamura, O. Ichii, Y. Kon The 63th annual meeting of the Japanese the Anatomical Society. 63th 2017年09月 [査読無し][通常論文]
  • Elucidation of mammalian reproductive - autoimmune coupling mechanism and analysis of pathological conditions caused by its failure.
    Y. Otani, O. Ichii, Y. H. A. Elewa, T. Nakamura The 35th Scientific annual joint meeting of Hokkaido Veterinary Anatomy 35th 2017年08月 [査読無し][通常論文]
  • “Histopathological correlations between the mediastinal fat associated lymphoid clusters and bleomycin-induced pneumonitis in mice.”
    Y. H. A. Elewa, Md. A. Masum, T. Nakamura, O. Ichii, Y. Kon The 159th Meeting of the Japanese Society of Veterinary Sciences. September 6-8, 2016. Kanagawa, Japan. 159th 2016年09月 [査読無し][通常論文]
  • “CD34-positive capillary decreased with the progression of kidney disease in mouse models.”
    Md. A. Masum, Y. H. A. Elewa, T. Nakamura, O. Ichii, Y. Kon The 159th Meeting of the Japanese Society of Veterinary Sciences, September 6-8, 2016. Kanagawa, Japan. 159th 2016年09月 [査読無し][通常論文]
  • “Histopathological evaluation of the possible protective role of royal jelly and cod liver oil against reproductive toxicity of tartrazine in adult male albino rats.”
    Y. H. A. Elewa, A. Moustafa, A.A. Mohamed, A. A.A. Galal, O. Ichii, Y. Kon The 8th international Toxicology symposium in Africa. Cairo, Egypt. 8th 2016年08月 [査読無し][通常論文]
  • Elewa Yaser, HOSNY Ali, ABDUL Masum Md, NAKAURA Teppei, NAKAURA Teppei, ICHII Osamu, KON Yasuhiro 日本獣医学会学術集会講演要旨集 159th 302 2016年08月 [査読無し][通常論文]
  • ABDUL Masum Md, Elewa Yaser, HOSNY Ali, NAKAMURA Teppei, NAKAMURA Teppei, ICHII Osamu, KON Yasuhiro 日本獣医学会学術集会講演要旨集 159th 308 2016年08月 [査読無し][通常論文]
  • 細谷実里奈, 中村鉄平, 中村鉄平, Elewa Yaser, HOSNY Ali, 市居修, 昆泰寛 日本獣医学会学術集会講演要旨集 159th 314 2016年08月 [査読無し][通常論文]
  • “Sex-related difference of the mediastinal fat associated lymphoid clusters in MRL/MpJ-Faslpr autoimmune disease model mice.”
    Y. H. A. Elewa, O. Ichii, Y. Kon The 11th Scientific Vet. Med. (second international) conference. El-Ain-El Sokhna, Egypt. 11th 2016年03月 [査読無し][通常論文]
  • THAI Truc Quynh, NGUYEN Huy Bang, SAITOH Sei, SAITOH Yurika, SHIMO Satoshi, ELEWA Yaser, Hosny Ali, ICHII Osamu, KON Yasuhiro, TAKAKI Takashi, JOH Kensuke, OHNO Nobuhiko 日本顕微鏡学会関東支部講演会予稿集 40th 26 2016年 [査読無し][通常論文]
  • A Novel Mouse Mediastinal Fat-associated Lymphoid Tissue and Its Relationship with Autoimmune Disease
    Y. H. A. Elewa, O. Ichii, S. Otsuka-Kanazaw, Y. Kon The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA). Bali, Indonesia 5th 2015年02月 [査読無し][通常論文]
  • YASER Elewa, ICHII Osamu, OTSUKA Saori, KANAZAWA Tomonori, RI Shingyo, NAMIKI Yuka, KON Yasuhiro, HASHIMOTO Yoshiharu 日本獣医学会学術集会講演要旨集 150th 160 2010年09月01日 [査読無し][通常論文]
  • ELEWA Yaser Hosny, BAREEDY Mohammad Hafez, ABUEL‐ATTA Ahmed Awad, ICHII Osamu, OTSUKA Saori, KANAZAWA Tomonori, LEE Shinhyo, HASHIMOTO Yoshiharu, KON Yasuhiro Jpn J Vet Res 58 (2) 121 -135 2010年08月 [査読無し][通常論文]

共同研究・競争的資金等の研究課題

  • Grant-in-Aid for Young Scientists B - 1
    研究期間 : 2017年01月 -2019年 
    代表者 : エレワ ヤセル

教育活動情報

主要な担当授業

  • 獣医科学基礎科目 生命科学特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院


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