研究者データベース

上田 宏一郎(ウエダ コウイチロウ)
農学研究院 基盤研究部門 畜産科学分野
教授

基本情報

所属

  • 農学研究院 基盤研究部門 畜産科学分野

職名

  • 教授

学位

  • 修士(農学)
  • 博士(農学)

ホームページURL

J-Global ID

研究キーワード

  • 栄養生態学   家畜生産システム   肉牛   ヒツジ   消化   飼料摂取量   ウマ   乳牛   放牧   

研究分野

  • ライフサイエンス / 実験動物学 / 畜産学
  • ライフサイエンス / 動物生理化学、生理学、行動学 / 畜産学

職歴

  • 2016年04月 - 現在 北海道大学 大学院農学研究院畜牧体系学研究室 教授
  • 2011年11月 - 2016年03月 北海道大学 大学院農学研究院畜牧体系学研究室 准教授
  • 2008年04月 - 2011年10月 北海道大学 大学院農学研究院家畜栄養学研究室 准教授
  • 2006年04月 - 2008年03月 北海道大学 大学院農学研究院家畜栄養学研究室 助教授
  • 2004年08月 - 2006年03月 北海道大学 大学院農学研究科家畜栄養学研究室 助教授
  • 1999年04月 - 2004年07月 北海道大学 大学院農学研究科畜牧体系学研究室 助手
  • 1998年01月 - 1999年03月 北海道大学 農学部畜産科学科畜牧体系学研究室 助手
  • 1996年09月 - 1997年12月 農林水産省畜産試験場 栄養部反芻家畜代謝研究室 (社)新技術協会 科学技術特別研究
  • 1996年04月 - 1996年08月 農林水産省畜産試験場 栄養部栄養第1研究室 非常勤職員

学歴

  • 1993年04月 - 1996年03月   北海道大学   農学研究科   畜産学専攻博士後期課程
  • 1991年04月 - 1993年03月   北海道大学   大学院農学研究科   畜産学専攻修士課程
  • 1988年10月 - 1991年03月   北海道大学   農学部   畜産学科
  • 1987年04月 - 1988年09月   北海道大学   教養部 理Ⅲ系
  • 1984年04月 - 1987年03月   私立大阪明星高等学校

所属学協会

  • 日本綿羊研究会   栄養生理研究会   日本家畜管理学会   北海道畜産草地学会   日本草地学会   日本畜産学会   

研究活動情報

論文

  • Furukawa E, Masaki T, Sakaguchi K, Bo M, Yanagawa Y, Ueda K, Nagano M
    Journal of Ovarian Research 13 1 7  2020年01月 [査読有り][通常論文]
  • Yuko Shingu, Koichiro Ueda, Shingo Tada, Tomohiro Mitani, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 88 8 1120 - 1127 2017年08月 [査読有り][通常論文]
     
    In this study, effects of grazing-experienced heifer presence on foraging behavior development at the feeding station (FS) scale for first-grazing season calves were determined. A group of four calves grazing alone (C), and another comprising four calves (Wc) and three grazing-experienced heifers (Wh), were alternately stocked every day for 2 h on the same pasture for 26 days. The foraging time budget for Wc was significantly longer than that for C (P < 0.05) on day 7, and was similar to that for Wh on all days. Bite rate per FS was significantly higher for Wc (15.5 bite/min) than for C (13.2 bite/min) from day 1 to 14 (33.4 vs. 29.0 bite/min, respectively; P < 0.01) and significantly lower than that for Wh on all days. The number of steps taken between adjacent FSs by Wc (4.7) was significantly lower than that for C (7.2) on days 1 and 14 (2.1 vs. 2.9 steps, respectively; P < 0.01) and was similar to Wh on all days. This suggests that grazing-experienced heifers have positive effects on the foraging behavior development of new-season grazing calves through 14 days after the start of stocking.
  • Skill reading of human social cues by horses (Equus caballus) reared under year-round grazing conditions
    Ryoko Koizumi, Tomohiro Mitani, Koichiro Ueda, Seiji Kondo
    Animal Behaviour and Management 53 69 - 78 2017年 [査読有り][通常論文]
  • Koichiro Ueda, Tomohiro Mitani, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 88 1 107 - 118 2017年01月 [査読有り][通常論文]
     
    The present study aimed to clarify the effect of timing and type of supplementary grain in grazing dairy cows on herbage dry matter intake (HDMI), nitrogen utilization and milk production. Eight lactating cows were allowed to graze from evening to morning during three seasonal periods (spring, summer, autumn). They were randomly allocated to four treatments (timing: pre- (Pre) or post-grazing (Post), for large grain allotments consisting of 75% of daily grain offered; grain type: barley or corn) in 4x4 Latin square designs in each period. In the spring period, HDMI was greater for cows fed corn than those fed barley (P=0.005), whereas cows in the Pre treatment had a similar HDMI, higher (P=0.049) urinary purine derivative concentration and greater (P=0.004) milk yield compared with cows in the Post treatment. In the summer and autumn periods, timing treatments did not affect HDMI, nitrogen utilization or milk production, but cows supplemented with barley had higher urinary purine derivatives concentration (P<0.05) and milk yield (P<0.05) compared with those supplemented with corn. The results indicate that large grain allotments immediately before evening grazing during early grazing seasons increased ruminal microbial protein synthesis and milk production without reducing HDMI regardless of grain type.
  • Koichiro Ueda, Tomohiro Mitani, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 87 10 1233 - 1243 2016年10月 [査読有り][通常論文]
     
    Two experiments were conducted to elucidate the effect of increased concentrate allotment before evening grazing on herbage intake, nitrogen utilization and rumen fermentation in dairy cows. In experiment 1, nine lactating cows were grazed in the morning and evening sessions (2.5 h each). The cows were allocated to treatments of three concentrate allotment levels before the evening grazing session by altering proportions to daily total offered; 25%, 50% and 75%. Daily herbage dry matter intake quadratically decreased with increasing the concentrate allotment levels (P < 0.05). Nitrogen utilization was similar among treatments. To investigate diurnal changes in rumen fermentation, a second experiment was conducted where six ruminally cannulated non-lactating dairy cows grazed in the morning and evening sessions (3 h each) were subjected to the same treatments as experiment 1. Total volatile fatty acid concentration in the rumen linearly increased with increasing the concentrate allotment levels throughout the pre-evening grazing session to the post-morning grazing session (P < 0.01). The results indicate that dairy cows reduce daily herbage intake but do not alter nitrogen utilization with increasing concentrate allotment before evening grazing.
  • Koichiro Ueda, Tomohiro Mitani, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 87 9 1148 - 1156 2016年09月 [査読有り][通常論文]
     
    To clarify the effect of digesta weight in the reticulorumen on diurnal and seasonal fluctuations in herbage intake, six ruminally cannulated, non-lactating dairy cows were grazed on perennial ryegrass/white clover pasture during morning and evening sessions in spring and autumn. The digesta weight of fresh matter, dry matter (DM) and fiber in the reticulorumen at the beginning and the end of each grazing session was lower in spring than in autumn (P<0.01). Although the digesta weight was similar between the sessions at the beginning of grazing, it was greater for the evening than for the morning at the end of grazing (P<0.01). The large particles proportion in the digesta was lower for the morning than the evening (P<0.01), and it was lower in spring than in autumn (P<0.01). The concentrations of volatile fatty acids in rumen fluid were generally higher in spring compared with autumn. The herbage DM intake during the evening was greater compared with the morning in both seasons (P<0.01). However, there was no difference in herbage DM intake between seasons. The results showed that the rumen digesta fill was not the sole factor explaining diurnal and seasonal variation of herbage intake in grazing dairy cows.
  • Koichiro Ueda, Tomohiro Mitani, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 87 9 1122 - 1129 2016年09月 [査読有り][通常論文]
     
    Two experiments were conducted to clarify the effect of water-soluble carbohydrate (WSC) content in orchardgrass pasture on the diurnal distribution of grazing time. Six ruminally cannulated, non-lactating dairy cows were grazed on either of two pastures with different orchardgrass cultivars containing low WSC (LWSC; cultivar: Hokkai 28') or high WSC (HWSC; cultivar: Harunemidori'). The cows were grazed in morning and evening sessions in experiment 1, whereas the cows were grazed throughout the day in experiment 2. In experiment 1, grazing time of the cows on HWSC was longer than that of the cows on LWSC (P < 0.01). This difference was larger in the morning session than in the evening session (pasture x grazing session: P < 0.05). Effects on herbage intake were similar to those on grazing time. In experiment 2, daily total grazing time was longer for the cows on HWSC than for those on LWSC (P < 0.05). The cows on HWSC spent a longer time grazing than those on LWSC in the morning between 03.00 and 09.00 hours (P < 0.01). The results indicated that prolonged grazing time in the period between dawn and early morning could increase daily herbage intake in cows grazed on pastures of orchardgrass cultivars with high-WSC content.
  • Koichiro Ueda, Tomohiro Mitani, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 87 8 997 - 1004 2016年08月 [査読有り][通常論文]
     
    This study aimed to clarify diurnal fluctuations of herbage intake, ruminal fermentation of herbage carbohydrates and proteins, and digesta particulate weight in the rumen of grazing dairy cows. Six ruminally cannulated, non-lactating dairy cows were grazed on perennial ryegrass/white clover pasture either in the morning (04.00 to 08.00 hours) or the evening (16.00 to 20.00 hours). Cows grazed in the evening spent more time (P<0.01) and consumed more herbage (P<0.01) compared with cows grazed in the morning. Higher (P<0.05) daily mean concentrations of total volatile fatty acid, propionate and n-butyrate in rumen fluid were observed for cows grazed in the evening compared with cows grazed in the morning. Although cows grazed in the evening ingested more crude protein compared with cows grazed in the morning, no significant difference in NH3-N concentration in rumen fluid was observed between them. The ratio of purine-derivative concentration to creatinine concentrations was higher (P<0.01) in the urine of cows grazed in the evening than in cows grazed in the morning. These results clearly indicated that evening grazing was advantageous for dairy cows compared with morning grazing, in terms of ruminal fermentable energy intake and nitrogen utilization efficiency.
  • Mitani T, Kobayashi, K, Ueda, K, Kondo, S
    Animal Science Journal 87 2 233 - 241 2016年02月 [査読有り][通常論文]
     
    Milk produced by the grazing system, referred to as grazing milk contains many components required for human health. The milk fatty acid (FA) profile is strongly associated with the diet on the farms. In the present study, based on the FA profile of farmer's bulk milk, we determined how to discriminate between milk produced on grazing and on a confinement system. A field survey was conducted four times (grazing and confinement season) in the Konsen (29 farms) and Okhotsk (25 farms) area in Hokkaido. Farmer's bulk milk samples and details of feeding management were collected and the FA profile of milk was measured. Milk produced during the grazing season contained less C16:0 and cis-9 C16:0, and more C18:0, cis-9 C18:1, trans-11 C18:1, cis-9,12 C18:2, cis-9,trans-11 C18:2 and cis-9,12,15 C18:3 than milk produced during the confinement season. Discrimination analysis using 16 FA revealed that almost all milk samples were discriminated correctly (confinement season: 90% correct and 10% borderline, grazing season: 88% correct, 9% borderline and 3% incorrect). For farmers that were categorized incorrectly and were considered borderline in the grazing season, the dependency on pasture was low compared with that for farmers correctly discriminated. Therefore, to claim grazing milk, a high dependency on pasture is required for grazing dairy farmers.
  • Regional difference of feeding management and milk composition in Hokkaido, Japan
    Tomohiro Mitani, Kuniyuki Kobayashi, Tetsushiro Endo, Koichiro Ueda, Seiji Kondo
    Proceedings of the 6th Korea-China-Japan Grassland Conference 86 - 89 2016年 [査読無し][通常論文]
  • Ibrahim F. Rehan, Koichiro Ueda, Tomohiro Mitani, Maho Amano, Hiroshi Hinou, Tetsu Ohashi, Seiji Kondo, Shin-Ichiro Nishimura
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63 48 10578 - 10590 2015年12月 [査読有り][通常論文]
     
    Because various stresses strongly influence the food productivity of livestock, biomarkers to indicate unmeasurable environmental stress in domestic animals are of increasing importance. Thermal comfort is one of the basic principles of dairy cow welfare that enhances productivity. To discover sensitive biomarkers that monitor such environmental stresses in dairy cows, we herein performed, for the first time, large-scale glycomics on 336 lactating Holstein cow serum samples over 9 months between February and October. Glycoblotting combined with MALDI-TOF/MS and DMB/HPLC allowed for comprehensive glycomics of whole serum glycoproteins. The results obtained revealed seasonal alterations in serum N-glycan levels and their structural characteristics, such as an increase in high-mannose type N-glycans in spring, the occurrence of di/triantennary complex type N-glycans terminating with two or three Neu5Gc residues in summer and autumn, and N-glycans in winter dominantly displaying Neu5Ac. A multivariate analysis revealed a correlation between the serum expression levels of these season-specific glycoforms and productivity.
  • Win K, Ueda, K, Kondo, S
    Animal Science Journal 86 9 833 - 841 2015年09月 [査読有り][通常論文]
     
    In this study, we aimed to evaluate the effects of six levels of orchardgrass hay (GH) proportion (0%, 10%, 20%, 30%, 40% or 50% of dry matter) in finely chopped corn silage (CS)-based diets on digesta kinetics of CS and GH in the rumen. Six non-lactating, rumen-cannulated Holstein cows were used in a 6x6 Latin square design. Ruminal digesta kinetics was measured by ruminal dosing of feed particle markers (dysprosium for CS, erbium for GH) followed by fecal sampling. The increase of GH proportion had a quadratic effect (P<0.01) on total tract digestibility of neutral detergent fiber (NDF) and acid detergent fiber. The proportion of GH did not affect the particle size distribution of rumen digesta, total weight of dry matter or NDF in the rumen. The rates of large particle size reduction in the rumen for CS tended to increase linearly with increasing GH proportion (P=0.077). A quadratic effect (P<0.05) was found with increasing the GH proportion for the ruminal passage rate of small GH particles, but not for CS particles. The results suggested that associative effects between CS and GH could be generated on rumen digesta kinetics when cows were fed a CS-based diet with an increased proportion of GH.
  • Effect of substitution of highly-digestible fiber of beet pulp for corn grain starch in concentrate on energy balance and recovery of ovarian function in early lactating dairy cows
    Bo M, Masaki, T, Nagano, M, Yanagawa, Y, Mitani, T, Ueda, K, Kondo, S
    Hokkaido Journal of Livestock and Grassland Science 3 45 - 53 2015年 [査読有り][通常論文]
  • TADA Shingo, UEDA Koichiro, NAKATSUJI Hiroki, KONDO Seiji
    Animal Behaviour and Management 50 2 61 - 68 2014年06月 [査読有り][通常論文]
     
    Under rotational grazing by dairy cows, effect of stocking rate on soil animal, soil microorganism, inorganic nitrogen contents in soil, herbage production and nitrogen intake were evaluated using five perennial ryegrassdominant pastures. Each pasture was grazed at once every fifteen days from early July to early October. The stocking rate of each pasture was 10, 20, 30, 40 and 50cow-hr/ha/day, respectively. In stocking rate 10-40 cow-hr/ha/day, the number of soil animals and herbage regrowth tended to increase with increasing the stocking rate, and consequently, herbage production and nitrogen intake increased, although soil microorganisms and inorganic nitrogen contents in soil had little differences. However, in stocking rate 50cow-hr/ha/day, herbage production and nitrogen intake were lower than in stocking rate 40cow-hr/ha/day, because herbage regrowth was lower than stocking rate 40 cow-hr/ha/day, and the number of soil animals and inorganic nitrogen contents in soil were the lowest in all stocking rate-treatment.
  • M. Miyaji, K. Ueda, H. Hata, S. Kondo
    JOURNAL OF ANIMAL SCIENCE 92 4 1574 - 1581 2014年04月 [査読有り][通常論文]
     
    Eight Thoroughbred horses were used to examine the effects of grass hay intake on the fiber digestion and the retention time of digesta in the total gastrointestinal tract and the hindgut segments. The horses were randomly assigned to 2 groups and offered 2.0 (high intake [HI]) or 1.3 kg DM/(100 kg BW.d; low intake [LI]) of timothy hay in equal amounts every 3 h for 17 d. The digestibility and total tract mean retention time of digesta (tMRT) in the total gastrointestinal tract were measured from d 11 to d 15. To measure the mean retention time of digesta in each hindgut segment (sMRT), the horses were euthanized on d 17, after being fed a series of hay markers that were labeled with different rare earth elements. The digesta were collected from the ileum, cecum, right ventral colon (RVC), left ventral colon (LVC), left dorsal colon, right dorsal colon (RDC), and small colon (SC) to measure fiber digestibility, sMRT, VFA concentration, and fibrolytic activities. The digestibility of NDF and ADF in the total gastrointestinal tract was lower (P < 0.01) for HI than for LI. The cumulative disappearance of NDF and ADF from the ileum to the LVC attained 90% of total tract digestion regardless of hay intake. Values did not differ according to treatments in the ileum and the cecum but were lower (P < 0.01) for HI than for LI in the postcecal segments. This was caused by the smaller increase in the cumulative NDF and ADF disappearance from the cecum to the RVC for HI than for LI. The solid phase tMRT was shorter (P = 0.04) for HI than for LI. Although sMRT in the RDC and SC were shorter (P = 0.04 and P < 0.01, respectively) for HI than for LI, no differences were observed in the other hindgut segments. There was no difference between treatments in the total VFA concentration, VFA proportions, or the specific activity of carboxymethyl cellulase and xylanase in each hindgut segment. The fibrolytic activity for the cecum and RVC were greatest and decreased beyond the LVC. A clear relation between mean retention time and fiber digestion was observed in the total gastrointestinal tract, but the segments where a difference in sMRT was detected because of the intake were not the same as those with the fiber digestibility depression. Although the reduction in total tract fiber digestibility with increased feed intake was clearly shown to occur between the cecum and RVC, which were the main segments for fiber digestion, it could be explained neither by the sMRT nor by the fibrolytic activity in these segments.
  • 北海道和種馬における林床植物のin vitro消化率と選択採食順位との関係
    内山 知, 上田宏一郎, 秦 寛, 近藤 誠司
    北海道畜産学会報 2 93 - 97 2014年 [査読有り][通常論文]
  • Effects of a proportion of herbage intake and crude protein content of herbage on nitrogen and energy utilization in lactating dairy cows
    Mitani, T, Ueda, K, Endo, T, Takahashi, M, Nakatsuji, H, Kondo, S
    Hokkaido Journal of Livestock and Grassland Science 2 51 - 62 2014年 [査読有り][通常論文]
  • 近藤誠司, 中辻浩喜, 上田宏一郎, 高橋誠
    農林水産省農林水産技術会議事務局研究成果 488 51 - 55 2013年01月 [査読無し][招待有り]
  • Shingo Tada, Makoto Takahashi, Koichiro Ueda, Hiroki Nakatsuji, Seiji Kondo
    Behavioural Processes 92 107 - 112 2013年01月 [査読有り][通常論文]
     
    Fractal analysis was applied to grazing paths in non-patchy environments to clarify the behavioral information available from the analysis and to evaluate the effect of social factors on grazing paths. The grazing paths of Holstein Friesian cows in groups of 4 (GROUP-4) and 10 cows (GROUP-10) on a spatially homogeneous pasture were observed. Application of fractal analysis could quantify three aspects of grazing paths of cows: (1) hierarchical scales, (2) structure and (3) sinuosity by scale. In this study, regardless of the group size, the hierarchical scale of grazing paths was approximately 10. m. The paths showed different patterns below and above the 10. m scale. For both group sizes, fractal dimension (an index of sinuosity) below the 10. m scale was smaller than fractal dimension above 10. m scale suggesting that the paths below the 10. m scale were straighter than that above the 10. m scale. The fractal dimensions below and above the 10. m scale were significantly larger for GROUP-4 than for GROUP-10 (P< 0.05). Sinuosity of paths of GROUP-4 was higher than that of GROUP-10 over the entire scale. From these three aspects, fractal analysis can clarify the factors related to grazing paths of herbivores. © 2012 Elsevier B.V.
  • Aye Sandar Cho, Koichiro Ueda, Seiji Kondo
    Animal Science Journal 83 9 650 - 655 2012年09月 [査読有り][通常論文]
     
    In vitro gas production (GP) method was used to investigate associative effects on ruminal digestion when grains (corn or barley) were supplemented to spring pasture and autumn pasture. Pasture (75%) was incubated with 25% corn or barley and gas production was monitored up to 96h. After incubation, the residues were used to determine dry matter and organic matter digestibility (DMD and OMD). Gas production parameters were analyzed by applying a single exponential equation. Organic matter effective degradability (OMED) was determined from GP parameters and OMD. The positive associative effects on the rate of GP, DMD, OMD and OMED were observed when spring pasture was incubated with corn. However, similar effects were not observed in a barley mixture. However, for autumn pasture, both corn and barley mixtures showed positive associative effects on rate of GP, OMD and OMED. The results of this study indicated that supplementation of corn would be better than barley for spring pasture with high water-soluble carbohydrate contents, whereas both grain supplementations were effective to obtain positive associative effects on the rate of GP and OMED for autumn pasture with high cell wall content. © 2012 The Authors. Animal Science Journal © 2012 Japanese Society of Animal Science.
  • Evaluation of associative effects on ruminal digestion kinetics between rice straw and protein supplements
    Cho, A, Ueda, K, Kondo, S
    北海道畜産学会報 54 47 - 52 2012年 [査読有り][通常論文]
  • Tin Ngwe, Yoko Nukui, Shinya Oyaizu, Genki Takamoto, Satoshi Koike, Koichiro Ueda, Hiroki Nakatsuji, Seiji Kondo, Yasuo Kobayashi
    ANIMAL SCIENCE JOURNAL 83 1 43 - 49 2012年 [査読有り][通常論文]
     
    This study evaluated the suitability of easily digested fiber sources as a supplemental fiber to improve overall fiber digestion in ruminants. First, the degradation of five fibrous feedstuffs and the stimulatory effects on rumen bacteria were examined in situ. Chickpea and lablab bean husks were selected for their potential use due to their large degradable fraction (> 94%), which had a stimulatory effect on fibrolytic rumen bacteria such as Fibrobacter succinogenes. Second, a possible improvement in the digestibility of rice straw diet by husk supplementation was monitored in vivo. Four dietary treatments comprising RS (rice straw and concentrate), CHM (RS supplemented with Myanmar chickpea husk), CHE (RS with Egyptian chickpea husk) and LH (RS with lablab bean husk) were allocated to four wethers. The digestibility of acid detergent fiber was 3.15.5% greater in CHM and LH than RS. Total volatile fatty acid concentration was higher in LH than other treatments. Acetate proportion was higher in LH than RS. Ruminal abundance of F. succinogenes was 1.31.5 times greater in CHM and LH than RS. These results suggest that bean husk supplementation, especially lablab bean husk, might improve the nutritive value of rice straw diet by stimulating fibrolytic bacteria.
  • Tomohiro Mitani, Koichiro Ueda, Tetsushiro Endo, Makoto Takahashi, Hiroki Nakatsuji, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 83 2 121 - 127 2012年 [査読有り][通常論文]
     
    Ten lactating cows were used to determine the effect of feeding non-fibrous carbohydrate (NFC) supplement before grazing on feed intake and nitrogen (N) utilization throughout a grazing season. The experiment was conducted from June to September. Cows grazed twice a day (2.5 h x 2) under a set stocking system and were fed NFC supplement (1 kg/4 kg of milk yield) 2 h before grazing (PRE) or immediately after grazing (POST). Cows were also fed a grass and corn silage mixture ad libitum. Herbage dry matter intake (DMI) was greater for PRE than for POST throughout the experiment and decreased from June to September. Conversely, silage DMI was less for PRE than for POST throughout the experiment and increased over the grazing season. Consequently, total DMI for PRE did not differ from that for POST. Milk urea-N concentration and urinary urea-N excretion in June did not differ between the treatments, whereas that after July was higher for PRE than for POST. Proportion of urinary N excretion to absorbed N intake in June was lower for PRE than for POST, but that after July was higher for PRE than for POST. Feeding NFC supplement before grazing would improve N utilization when cows eat large amounts of herbage high in N.
  • M. Miyaji, K. Ueda, H. Hata, S. Kondo
    ANIMAL FEED SCIENCE AND TECHNOLOGY 165 1-2 61 - 67 2011年04月 [査読有り][通常論文]
     
    The objective of this experiment was to investigate the effect of hay quality and form on mean retention time (MRT) of digesta and total tract apparent digestibility (TTAD) in horses fed under ad libitum or restricted conditions using four Hokkaido native horses. Two experiments (Exp.) were conducted using 4 x 4 Latin square designs with treatments arranged factorially with hay quality [high quality timothy hay harvested at late vegetative-stage (HH) or low quality timothy hay harvested at late bloom-stage (LH)] and physical form [chopped to a theoretical 10-mm length (G10H) or ground through a 5-mm sieve (G5H)] as main effects. In Exp. 1, horses were offered the experimental diets ad libitum whereas in Exp. 2 horses were offered a restricted allowance of 1.6 kg dry matter (DM) per 100 kg of BW per day. Under ad libitum conditions, hay quality, physical form and their interactions did not affect feed intake. In both Exp., solid phase digesta MRT in total tract and in the first compartment which having the longest MRT were longer (Exp. 1: P=0.08 and P=0.02, Exp. 2: P=0.04 and P=0.04, respectively) for G5H than for G10H, but hay form did not affect MRT in the second compartment which having the second longest MRT. Hay quality did not affect MRT. The MRT through the gastro-intestinal tract of the solid phase was 23.7 h in Exp. 1 and 27.1 h in Exp. 2. The TTAD of DM was higher (P < 0.01) for HH than for LH in both Exp. In horses fed HH, TTAD of DM was 0.487 in Exp. 1 and 0.561 in Exp. 2, whereas in horses fed LH was 0.355 in Exp. 1 and 0.359 in Exp. 2. Hay form did not affect TTAD of DM. The effects of diet on TTAD of fibre were similar to those observed for DM. The results indicate that MRT lengthen in horses when the proportions of small particle sizes in the diet are high. Feeding chopped hay instead of ground hay did not decrease TTAD of fibre even if total and/or segmental MRT were shortened. (C) 2011 Elsevier B.V. All rights reserved.
  • 平坦地での昼夜低地放牧における泌乳牛の移動距離の変化
    森 光生, 遠藤哲代, 中辻浩喜, 上田宏一郎, 近藤誠司
    北海道畜産学会報 53 43 - 50 2011年 [査読有り][通常論文]
  • 多様な植生をもつ林地に放牧した北海道和種馬のフィーディングステーション内における採食植物選択
    内山 知, 上田宏一郎, 中辻浩喜, 秦 寛, 近藤誠司
    北海道畜産学会報 53 35 - 41 2011年 [査読有り][通常論文]
  • 舎飼い飼養から放牧飼養への移行時期における牛乳成分の変動
    三谷朋弘, 佐藤悠二, 上田宏一郎, 高橋 誠, 中辻浩喜, 近藤誠司, 舎飼い飼養から放牧飼養への移行時期における牛乳成分の変動
    北海道畜産学会報 53 29 - 34 2011年 [査読有り][通常論文]
  • 泌乳牛の定置放牧および輪換放牧の違いが草地構造,牧草生産量および利用草量に及ぼす影響
    遠藤哲代, 三谷朋弘, 高橋 誠, 上田宏一郎, 中辻浩喜, 近藤誠司
    日本草地学会誌 55 9 - 14 2009年 [査読有り][通常論文]
  • 反芻胃でのタンパク質と炭水化物の分解同期化を考慮した放牧乳牛に対する併給飼料の給与法
    三谷朋弘, 遠藤哲代, 高橋 誠, 上田宏一郎, 中辻浩喜, 近藤誠司
    栄養生理研究会報 52 1 - 9 2009年 [査読有り][通常論文]
  • Makoto Miyaji, Koichiro Ueda, Yasuo Kobayashi, Hiroshi Hata, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 79 3 339 - 346 2008年06月 [査読有り][通常論文]
     
    We studied in vivo fiber digestibility and fermentation parameters such as volatile fatty acid concentration and in vitro degradation in each segment of hindgut of horses fed timothy hay or silage made from the same sward. Six Thoroughbred horses were fed timothy hay or silage in equal amounts (1.6% of bodyweight per day, bodyweight mean 572 kg) every 3 h per day, then slaughtered. There were no differences between hay and silage in the concentration of the total volatile fatty acids, the apparent digestibility of dry matter, organic matter, and fiber, and in vitro neutral detergent fiber (NDF) disappearance rate in each segment. The total volatile fatty acid concentration and the apparent digestibility of dry and organic matter and fiber differed (P < 0.01) depending on the digestive segments. Both for hay and silage, the concentration of total volatile fatty acids in the digesta liquid phase largely increased (P < 0.01) from the cecum to the right ventral colon, and kept a constant value from the right ventral colon to the right dorsal colon, and then decreased from the right dorsal colon to the small colon. For dry and organic matter and fiber components, the apparent digestibility changed in the same manner from former to hinder segments of the hindgut. Regardless of diet, dry and organic matter and fiber components showed lower values in the cecum and the right ventral colon and increased (P < 0.01) largely from the right ventral colon to the left dorsal colon, then kept constant values in segments to the rear of left dorsal colon. There were no differences in in vitro NDF disappearance among cecum, ventral colon and dorsal colon.
  • Makoto Miyaji, Koichiro Ueda, Hiroki Nakatsuji, Teruo Tomioka, Yasuo Kobayashi, Hiroshi Hata, Seiji Kondo
    ANIMAL SCIENCE JOURNAL 79 1 89 - 96 2008年02月 [査読有り][通常論文]
     
    Six Thoroughbred horses were used to determine the mean retention time (MRT) of digesta in the different segments of the hindgut. The horses were fed timothy hay or silage in equal amounts (1.6% bodyweight [BW]/day) every 3 h/day. Hay or silage labeled with the rare earth elements cerium, dysprosium, neodymium, ytterbium, lanthanum, samarium and praseodymium, were fed to the horses 36, 30, 24, 18, 12, 6 and 3 h before slaughter, respectively. Just after slaughter, digesta samples in the different segments were collected and determined for the contents of the rare earth elements. Using these data, a method to calculate the MRT in the different segments was proposed. There were no significant differences between diets in the MRT of each segment. The averaged MRT of hay and silage in the cecum, right ventral colon, left ventral colon, left dorsal colon, right dorsal colon and small colon was 2.9, 3.1, 5.9, 1.0, 4.0, and 4.0 h, respectively. The dry matter weight of the digesta was related to the MRT in the right ventral colon (r = 0.94, P = 0.005), left ventral colon (r = 0.87, P = 0.03), left dorsal colon (r = 0.98, P < 0.001) and right dorsal colon (r = 0.95, P = 0.004), but it was not related to the MRT in the cecum and small colon.
  • Akihiro Matsuura, Emiko Ohta, Koichiro Ueda, Hiroki Nakatsuji, Seiji Kondo
    Journal of Equine Science 19 1 9 - 18 2008年 [査読有り][通常論文]
     
    To obtain basic knowledge about selecting horses for therapeutic riding, the influence of equine conformation on rider oscillation and relationships between these factors and the evaluation on horses as the therapeutic riding were studied. Thirty-five riding horses were used. Equine conformation was estimated by 24 indices. Rider oscillation was measured by an accelerometer fixed at the rider's waist. The spatial position of the oscillation was estimated by a double integration of the acceleration. Horses were evaluated for therapeutic riding by a Riding for the Disabled Association instructor as a rider. Evaluations were on a scale of 1 to 5, with 5 being the highest score for 27 items. Horses were classified into 4 groups: the short and narrow (SN), short and wide (SW), tall and narrow (TN), and tall and wide (TW). The frequencies of rider oscillation both at walk and trot were higher (P< 0.01), and the vertical (P< 0.01) and longitudinal (P< 0.05) amplitudes at trot were smaller, on short horses than on tall horses. The vertical amplitude at walk was smaller (P< 0.05) and the lateral amplitude at trot was larger (P< 0.01) on wide horses than on narrow horses. Short horses could be used for the rider who requires side walkers. Wide horses could be used for relieving muscular tension and for the rider who could not maintain good balance on the horse. Short and wide horses should be suitable for therapeutic riding.
  • 泉 賢一, 鈴木 知之, 上田 靖子, 上田 宏一郎, 大久保 正彦
    日本畜産學會報 = The Japanese journal of zootechnical science 78 4 427 - 433 公益社団法人 日本畜産学会 2007年11月25日 [査読有り][通常論文]
     
    ルーメン内の充満状態と採食および反芻活動との関連を調査するために4頭のルーメンカニューレ装着メンヨウを用いて試験を実施した.飼料はアルファルファ乾草(ALF)およびイネ科乾草(GR)として,1日2回自由採食させた.ルーメン内容物は給与直前(0 h),給与後最初の採食期(first meal)終了直後(FM),給与後4時間および8時間に採取した.First mealの乾物採食量(DMI)は両乾草で等しく,総DMIはALFの方がGRを上回った.総採食時間は飼料間で差はなかったが,採食期(meal)出現回数はALFの方がGRよりも多かった.DMIあたりの反芻時間はALFの方がGRよりも短かった.ルーメン内DM総量はFMでは両乾草の値が一致した.ルーメン内大飼料片(>1.18mm)プールサイズの推移は両飼料とも近似しており,0 hからFMにかけて急増した後に減少した.以上の結果から,草種にかかわらずfirst mealはルーメン内の充満によって終了すると考えられた.
  • Nakatsuji, H, Nishimichi, Y, Yayota, M, Takahashi, M, Ueda, K, Kondo, S, Okubo, M
    Grassland Science 52 175 - 180 2006年 [査読有り][通常論文]
  • Tomohiro Mitani, Makoto Takahashi, Koichiro Ueda, Hiroki Nakatsuji, Seiji Kondo
    Animal Science Journal 76 5 453 - 460 2005年10月 [査読有り][通常論文]
     
    Four Holstein cows were used to determine the effect of timing of the feeding of a corn silage (CS)-based supplement on the feed intake, milk production and nitrogen utilization of grazing dairy cows. The cows were fed the supplement 2 h before grazing (pre-grazing) or immediately after grazing (post-grazing). Cows were grazed for 5 h per day under a rotational grazing system. There was no difference in the herbage and total feed intake between treatments. The milk protein yield for pre-grazing tended to be higher than that for post-grazing, whereas the milk yield did not differ between treatments. The total nitrogen intake for pre-grazing tended to be higher than that for post-grazing (P = 0.06). There was no difference in the urinary nitrogen output between treatments, whereas the proportion of urinary nitrogen output : total nitrogen intake for pre-grazing tended to be lower than that for post-grazing (P = 0.06). The milk nitrogen output and nitrogen retention for pre-grazing tended to be higher than that for post-grazing (milk nitrogen, P = 0.06 nitrogen retention, P = 0.05). Nitrogen utilization of grazing dairy cows was improved by feeding a CS-based supplement before grazing.
  • Tomohiro Mitani, Makoto Takahashi, Koichiro Ueda, Hiroki Nakatsuji, Seiji Kondo, Masahiko Okubo
    Animal Science Journal 76 4 331 - 337 2005年08月 [査読有り][通常論文]
     
    The effects of supplementary corn silage (CS) of either 2 or 4 kg of dry matter (DM S + 2 and S + 4, respectively) above the energy requirement for milk production and maintenance for grazing dairy cows (S) were determined. Time-restricted grazing was used to compare the feed intake, milk production, and nitrogen and energy use of lactating cows. The experiment was carried out on two different pastures using a 3 × 3 Latin square design for each pasture. Cows were grazed for 5 h on a rotational grazing system and were fed concentrate (1 kg per 5 kg of milk yield). Herbage intake was measured using a weighing technique. To calculate the energy and nitrogen use, whole feces and urine were collected. There was no statistical effect of the pastures. Herbage intake decreased by the addition of CS (P= 0.02). The reduction of herbage DM intake per unit consumption of supplementary CS towards the S group were 0.80 and 0.45 kg for the S + 2 and S + 4 groups, respectively. The total DM intake for the S + 4 group was higher than that for the S and S + 2 groups (P = 0.02). Milk yield did not differ among treatments, even though the total DM intake for the S + 4 group was higher than that of the S and S + 2 groups. Nitrogen and energy use did not differ with the addition of CS.
  • 高橋誠, 上田宏一郎, 中辻浩喜, 近藤誠司
    日本草地学会誌 51 2 157 - 162 2005年07月 [査読有り][通常論文]
  • JJ Loor, K Ueda, A Ferlay, Y Chilliard, M Doreau
    ANIMAL FEED SCIENCE AND TECHNOLOGY 119 3-4 203 - 225 2005年04月 [査読有り][通常論文]
     
    Ruminal hydrogenation and duodenal flow of fatty acids were evaluated in three lactating Holstein cows fed a diet with a high concentrate: forage ratio (65:35) plus 5.0g/100g dry matter sunflower oil (SO), 5.0g/100g linseed oil (LO), or 2.5g/100g fish oil (FO). A 3 x 3 Latin square with 4-week periods was used. Grass hay was the forage. Biohydrogenation of 18:2n-6 was greater with SO (92%) than LO (0.85), and was lowest with FO (0.75). Cows fed LO had greater 18:3n-3 hydrogenation (0.95) compared with FO or SO (84%). Biohydrogenation of 20:5n-3 (EPA) and 22:6n-3 (DHA) with FO averaged 0.92 and 0.89. Flow of total conjugated linoleic acids (CLA) was greater in cows fed SO (8.3 g/d) compared with FO (4.0 g/d). Feeding LO resulted in flow of 6.9 g total CLA/d. Percentage of cis9trans11-CLA in duodenal lipids was 0.22 g/100 g total fatty acids with SO but only 0.06 or 0.13g/100 g with FO or LO. trans10,cis12-CLA percentage was greater with SO (0.19 g/100g) than FO or LO (0.04g/100 g). Flow of total non-conjugated 18:2 isomers averaged 99.4 g/d with LO and 60.3 g/d with FO or SO. Percentage of trans11,cis15-18:2, derived from 18:3n-3 hydrogenation, ranked by treatment was LO (4.70g/100g) > FO (2.70g/100g)> SO (0.86g/100g). Total trans-18:1 flow did not differ due to diets (254 g/d). trans10-18:1 percentage was greater in cows fed FO or SO (12.2 g/100g) than LO (3.1 g/100 g). trans 11-18:1 percentage averaged 10.8g/100 g across diets. Flow of 18:0 was 3.8-fold greater in cows fed SO or LO (373 g/d) than FO (96 g/d). Apparent intestinal digestibility (proportion of duodenal flow) of trans9- through trans12-18:1 was greater with FO (range 0.94-0.96) than SO (0.91-0.93), and intermediate with LO (0.92-0.95). Digestibility of cis9,trans11-CLA was greater in cows fed SO (0.85) compared with FO (0.63) or LO (0.79). trans10,cis12-CLA digestibility was greater with FO or SO (0.97) than LO (0.86). Dietary EPA and DHA from fish oil (at only 0.16 of total fatty acid intake) inhibited the reduction of trans-18:1 to 18:0 during hydrogenation of unsaturated fatty acids in the basal diet and led to marked accumulation of trans10-18:1. Intake of 18:2n-6 with FO was not associated with greater flow of trans10-18:1. The effect of FO on biohydrogenation may have been associated with a combination of factors such as greater numbers of ruminal protozoa and(or) increased isomerization of trans11-18:1 to trans10-18:1 in the rumen. (C) 2005 Elsevier B.V. All rights reserved.
  • Effect of pre-grazing feeding of a non-fibrous carbohydrate on feed intake, milk production and nitrogen utilization
    Proceedings of the, h Asian Diary Conference
    Tomohiro Mitani, Junki Honma, Tetsushiro Endo, Koichiro Ueda, Hiroki Nakatsuji, Seiji Kondo 31 - 37 2005年 [査読無し][通常論文]
  • 高橋 誠, 三嵜健司, 上田宏一郎, 中辻浩喜, 宿野部猛, 近藤誠司
    日本家畜管理学会誌 40 155 - 160 2005年 [査読有り][通常論文]
  • High-concentrate diets and polyunsaturated oils alter trans and conjugated isomers in bovine rumen, blood, and milk
    Loor, J. J, Ferlay, A, Ollier, A, Ueda, K, Doreau, M, Chilliard, Y
    Journal of Dairy Science 33 3986 - 3999 2005年 [査読有り][通常論文]
  • JJ Loor, K Ueda, A Ferlay, Y Chilliard, M Doreau
    JOURNAL OF DAIRY SCIENCE 87 8 2468 - 2471 2004年08月 [査読有り][通常論文]
     
    Trans-18: 1 and 18: 2 isomer composition in ruminal fluid during the daily feeding cycle was examined in 3 cows fed a high concentrate diet (35: 65) with 5% (DM basis) sunflower oil (SO), 5% linseed oil (LO), or 2.5% fish oil (FO) in a 3 x 3 Latin square with 3 4-wk periods. Grass hay and concentrate mixtures were fed at 0900, 1300, and 1700 h daily. Ruminal fluid was collected at 0900, 1100, 1300, 1500, 1700, 2000, and 0000 h. Feeding SO resulted in the greatest mean concentrations (% of total fatty acids) of trans10, cis12-18: 2 and cis9, trans11-18: 2. In particular, trans10, cis12- 18: 2 with SO was greater at 1500 (0.29%), 2000 (0.34%), and 0000 h (0.25%) relative to 0900 h (0.07%). Cis9, trans11-18: 2 concentration increased from 0.47% at 0900 h to a peak of 2.06% at 1100 h; it remained greater than the percentage determined at 0900 h at 1300 (1.4%) through 0000 h (1.1%). Concentration of trans11, cis15-18: 2 was greatest with LO, ranging from 3.3% ( 0900 h) to a peak of 11.4% at 2000 h. Mean trans10-18: 1 concentration ranked by diet was SO > FO > LO. Peak trans10-18: 1 with SO was observed at 1700 h (14.9%) compared with 0900 h (5.1%). Trans11-18: 1 did not differ with diet or time. Stearic acid decreased over time with all diets reaching minimum concentrations at 1700 to 2000 h relative to 0900 h. Feeding FO, however, decreased mean 18: 0 concentration 4-fold compared with LO or SO. The moderate effect on concentration of trans-18: 1 coupled with accumulation of 18: 2 intermediates and the decrease of 18: 0 over time suggest that oils reduced the biohydrogenation of 18: 2 isomers to trans-18: 1.
  • JJ Loor, K Ueda, A Ferlay, Y Chilliard, M Doreau
    JOURNAL OF DAIRY SCIENCE 87 8 2472 - 2485 2004年08月 [査読有り][通常論文]
     
    Duodenal flows of hydrogenation intermediates in response to changes in dietary forage: concentrate ratio ( F: C) and linseed oil were evaluated using 4 lactating Holstein cows fed a low ( 65: 35 forage to concentrate) or high ( 35: 65) concentrate diet without (LC, HC) added oil or with linseed oil (LCO, HCO) at 3% of DM. A 4 x 4 Latin square design was implemented for 5 wk. Lower hydrogenation of 18: 2n-6 and 18: 3n-3 was observed with HC, but it increased with LCO or HCO. Duodenal flow of total conjugated linoleic acids (CLA) increased by 1.40 (LCO) to 3.01 (HCO) g/d with linseed oil. This response was associated with greater flows of cis9, trans11- (+ 0.21 to + 0.55 g/d), trans11, cis13- (+ 0.33 to + 0.36), trans11, trans13- (+ 1.01 to + 1.15 g/d), and trans, trans-CLA (+ 0.12 to + 0.72 g/d). Trans10, cis12-CLA flow averaged 0.08 g/d and was not affected by F: C or oil. trans11, cis15-18: 2 flow increased by 8.5 ( LCO) to 62 (HCO) g/d in response to linseed oil. Total trans-18: 1 flow was 37 g/d in cows fed LC and increased to 81 g/d with HC. Feeding oil increased total trans-18: 1 to the greatest extent with HCO. Flow of trans10-18: 1 was lower with LC than with HC (1.46 vs. 20 g/d). Linseed oil increased trans11- 18: 1 flow by 40 ( LCO) to 113 g/d ( HCO). Feeding LCO and HCO also increased flows of trans6+7+8-, trans13+14-, trans15-, and trans16-18: 1. Apparent intestinal digestibility of trans-18: 1 isomers was largely unaffected by concentrate level and ranged between 67 and 95%. Linseed oil increased digestibility of nearly all isomers by 3 to 16 percentage units. Digestibility of cis9, trans11- CLA was greater in cows fed HC (55%) compared with cows fed LC (32%) and was not affected by linseed oil. Data suggest that high concentrate diets enhanced ruminal outflow of trans10- 18:1. We provide initial in vivo evidence that supplemental 18: 3n-3 is hydrogenated to trans11, cis15-18: 2, trans11-18: 1, trans13+14-18: 1, trans15-18: 1, trans6+7+8- 18: 1, and trans16-18: 1 primarily.
  • ウマにおける消化管内飼料片の粒度分布と繊維成分含量
    上田宏一郎, 宮地 慎, 小林泰男, 秦 寛, 近藤誠司, 大久保正彦
    北海道畜産学会報 46 79 - 82 2004年 [査読有り][通常論文]
  • 宮地 慎, 上田宏一郎, 山田文啓, 小林泰男, 秦 寛, 中辻浩喜, 近藤誠司
    日本畜産学会報 74 567 - 572 2004年 [査読有り][通常論文]
  • K Ueda, A Ferlay, J Chabrot, JJ Loor, Y Chilliard, M Doreau
    JOURNAL OF DAIRY SCIENCE 86 12 3999 - 4007 2003年12月 [査読有り][通常論文]
     
    The effect of linseed oil (LSO) supplementation on total-tract and ruminal nutrient digestibility, N metabolism, and ruminal fluid characteristics was investigated in dairy cows fed diets containing different forage to concentrate ratios ( F: C). The experimental design was a 4 x 4 Latin square with 2 x 2 factorial arrangement of treatments. Four lactating Holstein cows were fed a forage-rich diet without LSO ( F; F: C = 65: 35, dry matter basis), a forage-rich diet with LSO (FO; F: C = 65: 32, 3% LSO), a concentrate-rich diet without LSO ( C; F: C = 35: 65), or a concentrate-rich diet with LSO ( CO; F: C = 35: 62, 3% LSO). Total-tract digestibility of DM and OM was greater with supplemental LSO. A tendency for greater total-tract digestibility of NDF and ADF also was observed in cows fed LSO. Ruminal digestibility of NDF or ADF decreased when CO was fed compared with C. In contrast, feeding FO increased NDF or ADF digestibility compared with F. Although ruminal starch digestion was nearly complete with all diets, digestibility was greater when cows were fed C or CO compared with F or FO. Bacterial N flow to the duodenum decreased when FO was fed compared with F. In contrast, feeding CO increased bacterial-N flow compared with C. Neither F: C nor LSO supplementation affected ruminal pH or total VFA concentration in ruminal fluid. However, molar proportion of propionate was greater with C or CO compared with F or FO and increased with LSO supplementation regardless of F: C. Molar proportion of n-butyrate decreased with LSO supplementation. Total protozoal numbers in ruminal fluid decreased markedly only when CO was fed. Overall, data show that feeding LSO had no negative effects on total-tract digestion in dairy cows but may decrease ruminal fiber digestibility when fed with high-concentrate diets. The widely spread idea that LSO decreases digestibility, arising from studies with sheep, did not seem to apply to lactating cows fed 3% LSO.
  • J Pan, S Koike, T Suzuki, K Ueda, Y Kobayashi, K Tanaka, M Okubo
    ANIMAL FEED SCIENCE AND TECHNOLOGY 106 1-4 69 - 79 2003年04月 [査読有り][通常論文]
     
    Two in situ experiments were completed to study effects of mastication on particle-associated microbial mass and fibrolytic enzyme activity. In experiment 1, orchardgrass hay stems were cut into lengths of 10 cm (L, long), 2 cm (S, short) or ground to pass a I cm screen (G, ground), and offered to an esophageally fistulated steer in order to collect ingesta (LC, long and chewed; SC, short and chewed; GC, ground and chewed). These three forms of chewed ingesta and S (non-chewed short stem) were incubated for 2, 6, 14, 24, 48 or 96 h in the rumen of three wethers fitted with ruminal cannulae to which orchardgrass hay was fed. Chewed stem (LC, SC and GC) had higher fibrolytic enzyme activity from stem-associated microbes, higher rates and extents of neutral detergent fiber (NDF) disappearance, and shorter lag times than S (P < 0.05). LC had a higher rate and extent of NDF disappearance than GC and SC (P < 0.05). In experiment 2, tested materials were G (as in experiment 1), GC, reground GC (GCG, ground, chewed and reground; simulated rumination) and G treated with NaOH (G-NaOH). These materials were incubated in the rumen of three wethers for 2, 6 or 24 h. GC incubated in the rumen had higher concentrations of chitin (a fungal marker; P < 0.05) and tended to have higher concentrations of diaminopimelic acid (DAPA, a bacterial marker; P = 0.13), and higher colonization of Fibrobacter succinogenes (P < 0.05) compared with G. GCG had a higher specific surface area, a higher number of F. succinogenes (P < 0.05) and higher activities of fibrolytic enzymes (i.e. total and specific xylanase at 6 and 24 h, P < 0.15), when compared to GC. G-NaOH had the highest NDF disappearance (41.7%, P < 0.05) of the four stem types. Results show that chewing promotes fibrolytic microbial attachment, colonization and subsequent production of enzymes for ruminal fibrolysis by increasing physical damage, reducing particle size and increasing the surface area of the feed particles. (C) 2003 Elsevier Science B.V. All rights reserved.
  • J Pan, T Suzuki, S Koike, K Ueda, Y Kobayashi, K Tanaka, A Okubo
    ANIMAL FEED SCIENCE AND TECHNOLOGY 104 1-4 13 - 27 2003年02月 [査読有り][通常論文]
     
    Effects of urea infusion on the endoglucanase (CMCase) and xylanase activities in solid and liquid fractions of rumen contents in steers were investigated. Three steers were fed timothy hay once daily for 46 days that were divided into two periods, control period followed by urea infusion period. In the urea infusion period, urea was continuously infused into the rumen (5.7 g/h) after dissolving in water. Urea infusion increased ruminal ammonia-N from 4.8 to 13.7 mg/dl (P < 0.05). Although solid fraction consistently exhibited much greater activities of CMCase and xylanase than liquid fraction (P < 0.05), response to urea infusion was somewhat different between these two fractions. Activities of fibrolytic enzyme (CMCase and xylanase) in the rumen digesta were increased by urea infusion, and then the extent of the increase was higher for liquid than for solid fractions. Maximum values for liquid-associated xylanase occurred at 12 h after feeding, while those for the solid-associated occurred at 24 h (P < 0.05). Although urea. infusion decreased diaminopimelic acid (DAPA) concentration in solid fraction of the ventral digesta, relative abundance of Fibrobacter succinogenes in the ventral digesta. tended to increase when urea was infused. Regardless of urea infusion, activities of fibrolytic enzyme from solid fractions were higher in ventral sac than dorsal sac, while opposite results were obtained in liquid fractions. These results indicate that increased ruminal ammonia-N by urea infusion could enhance fibrolytic enzyme activity of liquid-associated microbes at an early stage after feeding, and those of particle-associated microbes at a later stage, through stimulating growth of cellulolytic microbes. (C) 2003 Elsevier Science B.V. All rights reserved.
  • Continuous monitoring of oxygen consumption in sheep head and estimation of energy expenditure from oxygen consumption
    Higuchi, K, Nishida, T, Enishi, O, Purnomoadi, A, Ueda, K, Terada, F
    Bulletin of National Institute of Livestock and Grassland Science 4 7 - 13 2003年 [査読有り][通常論文]
  • 宮地 慎, 上田宏一郎, 秦 寛, 塙 友之, 近藤誠司, 大久保正彦
    日本畜産学会報 74 213 - 219 2003年 [査読有り][通常論文]
  • ASM Selim, J Pan, T Suzuki, K Ueda, Y Kobayashi, K Tanaka
    ANIMAL FEED SCIENCE AND TECHNOLOGY 102 1-4 207 - 215 2002年12月 [査読有り][通常論文]
     
    An experiment was conducted to quantify postprandial changes in particle attaching ruminal bacteria in sheep fed untreated (UTS) and ammoniated (ATS) rice straw. Diaminopimelic acid (DAPA) was used as a marker for the calculation of mass of tightly associated bacteria (TAB). ATS feeding decreased large particle proportion, while increased fine particle proportion. Ammoniation of rice straw had a significant effect on mass of non-associated bacteria (NAB), loosely associated bacteria (LAB) and tightly associated bacteria (TAB) with feed particles, showing the higher values in ATS-fed sheep within 2-5 h after feeding. Significantly increased bacterial densities associated with small and fine particles were also observed, indicating that size of particles is an important factor for the attachment of bacteria. The current study suggests that ammoniation accelerates particle size reduction of rice straw in the rumen, stimulating colonization of bacteria onto the smaller particles and overall degradation of rice straw fragments. (C) 2002 Elsevier Science B.V. All rights reserved.
  • 西田武弘, 樋口浩二, 上田宏一郎, 栗原光規, Agung PURNOMOADI, 柴田正貴, 寺田文典
    日本畜産学会報 73 265 - 271 2002年 [査読有り][通常論文]
  • 鈴木知之, 潘 軍, 上田宏一郎, 田中桂一, 大久保正彦
    日本畜産学会報 72 343 - 350 2001年 [査読有り][通常論文]
  • Ueda, K, Ichinohe, T, Okubo, M, Asahida, Y
    Animal Science Journal 72 306 - 314 2001年 [査読有り][通常論文]
  • Pan, J, Suzuki, T, Ueda, K, Tanaka, K, Okubo, M
    Animal Science Journal 72 209 - 217 2001年 [査読有り][通常論文]
  • Ueda, K, Ichinohe, T, Tamura, T, Okubo, M, Asahida, Y
    Animal Science Journal 72 198 - 208 2001年 [査読有り][通常論文]
  • J Pan, T Suzuki, K Ueda, K Tanaka, M Okubo
    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES 13 12 1708 - 1716 2000年12月 [査読有り][通常論文]
     
    A study was made of diurnal changes in the ruminal bacteria associated with feed particles, i.e., non-associated (NAB), loosely associated (LAB), and tightly associated with particles (TAB), and the TAB concentration in different particle sizes from sheep fed orchardgrass (OG) or alfalfa (ALF) hay. Diaminopimelic acid (DAPA) was used to determine the TAB mass. Results showed that the bacterial masses in NAB and LAB were small, but comprised over 90% in TAB. The TAB mass in the ALF group sharply increased within 2 h after feeding and decreased afterward. The TAB mass showed the same trend in the OG group, increasing from 0 h to 2 h, but remained at the same level up to 14 h after feeding. The peak bacterial mass was, however, lower in the OG than the ALF group. The TAB concentration reflected the changes in total particulate tightly associated bacterial masses in both groups of hay fed sheep. Number of bacterial colonies per particle increased as the particulate size decreased in both groups. This difference, however, tended to decline as the postprandial period was prolonged. DAPA, however, tended to overestimate the TAB mass in the reticulo-rumen digesta of the hay fed sheep.
  • 鈴木 知之, 潘 軍, 泉 賢一, 上田 靖子, 上田 宏一郎, 田中 桂一, 大久保 正彦
    日本畜産學會報 = The Japanese journal of zootechnical science 71 9 J331 - J337 2000年08月25日 [査読有り][通常論文]
  • 反芻胃内飼料片付着微生物量およびin situ NDF消失率に及ぼす飼料片の粒度および給与乾草の影響
    潘 軍, 鈴木知之, 上田宏一郎, 田中桂一, 大久保正彦
    北海道畜産学会報 42 19 - 24 2000年 [査読有り][通常論文]
  • 乳牛におけるイタリアンライグラスロールベールラップサイレージの自由採食量と飼料成分,第一胃内滞留時間,消化率,消化速度との関係
    甘利雅拡, 梅田剛利, 上田宏一郎, 柾木茂彦, 寺田文典, 阿部 亮
    Grassland Science 46 254 - 260 2000年 [査読有り][通常論文]
  • Purnomoadi, A, Batajoo, K. K, Nonaka, I, Higuchi, K, Ueda, K, Enishi, O, Nishida, T, Kurihara, M, Terada, F
    Animal Science Journal 70 343 - 348 1999年 [査読有り][通常論文]
  • A Purnomoadi, KK Batajoo, K Ueda, F Terada
    INTERNATIONAL DAIRY JOURNAL 9 7 447 - 452 1999年 [査読有り][通常論文]
     
    Milk samples from two experiments (n = 506 in total) were analyzed by measurement of near-infrared (NIR) spectra to investigate the effect of feed source on milk fat and protein content. Milk samples from the first experiment (n = 300) were used for development of the calibration equation and validation of the equation. The calibration equations thus developed in the first experiment were then used in the second experiment to examine the applications of NIR measurement. All feeding trials used the same basal rations, which consisted of corn silage, Italian ryegrass, Alfalfa haycube, corn flake and commercial concentrate. The first experiment was comprised of three rations: (1) basal ration, (2) basal ration with soybean meal (48% of total crude protein (CP)) and (3) basal ration with soybean meal (19% of total CP) and fish meal (25% of total CP). The second experiment was comprised of five supplement alternatives: (1) no supplement (NS), (2) corn gluten meal (CGM, 26% of total CP), (3) fish meal (FM, 26% of total CP), (4) defatted soybean meal (SBM, 28% of total CP) and (5) roasted soybean meal (RSBM, 26% of total CP). Feeding regimes in both experiments were adjusted to fulfill the maintenance and production requirements. The results showed that NIR prediction of milk fat content was not influenced by the feed of animals, while the accuracy of protein prediction was significantly affected by the kind of feedstuff used in the ration. Thus, a wide range of milk samples from cows on clearly defined feeding regimes is necessary for developing a satisfactory calibration for NIR systems. (C) 1999 Elsevier Science Ltd. All rights reserved.
  • 中井文徳, 平嶋喜典, 上田宏一郎, アグン プルノモアディ, 樋口浩二, 永西 修, 寺田文典
    日本畜産学会報 70 390 - 396 1999年 [査読有り][通常論文]
  • 乾草の自由摂取量と反芻胃内飼料片の粒度別動態との関連
    上田 宏一郎
    北海道大学農学部邦文紀要 20 1 - 57 1997年 [査読有り][通常論文]
  • Ueda, K, Ichinohe, T, Tamura, T, Okubo, M, Asahida, Y
    Animal Science and Technology (Japan) 68 917 - 925 1997年 [査読有り][通常論文]
  • 自由採食下のめん羊における採食・反芻行動と反芻胃内粒度別飼料片の動態の関連
    泉 賢一, 岡部靖子, 福間 哲, 上田宏一郎, 大久保正彦
    北海道畜産学会報 38 94 - 97 1996年 [査読有り][通常論文]
  • 上田宏一郎, 一戸俊義, 田村 忠, 大久保正彦, 朝日田康司
    日本畜産学会報 66 949 - 956 1995年 [査読有り][通常論文]
  • Effect of orchardgrass growth stage on pool size and kinetics of digesta particles in the rumen of sheep
    Ichinohe, T, Tamura, T, Ueda, K, Okubo, M, Asahida, Y
    Asian-Australasian Journal of Animal Science 8 267 - 273 1995年 [査読有り][通常論文]
  • 刈取り時期, 草種の異なる乾草を給与しためん羊における反芻胃および十二指腸内容物の粒度分布
    上田宏一郎, 一戸俊義, 田村 忠, 大久保正彦, 朝日田康司
    北海道畜産学会報 36 41 - 44 1994年 [査読有り][通常論文]
  • Ichinohe, T, Tamura, T, Ueda, K, Okubo, M, Asahida, Y
    Animal Science and Technology (Japan) 65 701 - 708 1994年 [査読有り][通常論文]
  • 一戸俊義, 田村 忠, 上田宏一郎, 大久保正彦, 朝日田康司
    日本綿羊研究会誌 29 45 - 50 1992年 [査読有り][通常論文]

書籍

  • 飼料設計・給餌の基本と実践ー粗飼料生産から牛群マネジメントまで
    上田宏一郎 (範囲:第Ⅱ章 飼料設計の実践と応用 7パターン別の飼料設計④~放牧飼養型)
    デーリィマン社 2020年10月
  • 動物衛生学
    上田 宏一郎 (担当:分担執筆範囲:第10章 生産動物の衛生管理 6 特用家畜の衛生管理 5)めん羊と山羊)
    文栄堂出版 2018年04月
  • 日本飼養標準・乳牛(2017年版)
    上田 宏一郎 (担当:共著範囲:飼料中の繊維)
    中央畜産会 2017年11月
  • デーリィ・プロフェッショナル vol.4
    上田 宏一郎 (担当:共著)
    デーリィ・ジャパン社 2016年02月
  • 乳牛栄養学の基礎と応用
    上田 宏一郎 (範囲:脂質)
    デーリィ・ジャパン社 2010年
  • 子牛の科学
    上田 宏一郎 (範囲:第一胃の発達と離乳)
    チクサン出版社 2009年
  • 新版 酪農用語解説 第2版
    上田 宏一郎 (担当:共著)
    デーリィ・ジャパン社 2008年
  • 日本飼養標準・乳牛(2006年版)
    上田 宏一郎 (担当:共著範囲:飼料中の繊維)
    中央畜産会 2007年
  • ルーメン6~ルーメン機能を理解する実践栄養学~
    上田 宏一郎 (担当:共著範囲:反芻を科学する)
    デーリィ・ジャパン社 2001年

講演・口頭発表等

  • 搾乳ロボットを利用した放牧酪農経営における乳牛の草地利用と課題  [招待講演]
    上田 宏一郎
    道北地域考える酪農セミナー~道北地域での豊かな暮らしのために~ 2018年11月 口頭発表(基調)
  • 健全な乳牛を育成するための新たな飼養管理技術の開発  [招待講演]
    上田 宏一郎
    ランダ経済使節団来日記念セミナー「酪農・馬鈴薯セクターのコスト削減と効率化―日蘭協力の可能性を模索して」 2018年10月 口頭発表(招待・特別)
  • Timing strategy for concentrate supplementation in grazing dairy cows  [招待講演]
    上田 宏一郎
    The 7th Japan-China-Korea Grassland Conference 2018年07月 口頭発表(招待・特別)
  • 新しい北海道の酪農~生産寿命を延ばす哺乳・育成の可視化技術の開発を目指して~  [招待講演]
    上田 宏一郎
    平成30年度GTBH総会・理事会 記念講演会 2018年06月 口頭発表(招待・特別)
  • 草からの乳生産量  [招待講演]
    上田 宏一郎
    創地農業21第34回グラスファーミングスクール 2017年05月 口頭発表(基調)
  • 放牧家畜の食草行動  [招待講演]
    上田宏一郎
    アフリカの牧畜地域で活用しうるICT関連技術に関する研究集会 2014年03月 口頭発表(招待・特別)
  • 泌乳牛の放牧における基礎栄養学  [招待講演]
    上田宏一郎
    創地農業21第26回グラスファーミングスクール 2013年06月 口頭発表(基調)
  • 放牧時の栄養管理  [招待講演]
    上田宏一郎
    北海道公共牧場会平成22年度春季研修会 2010年04月 口頭発表(基調)
  • 基本的な放牧時の栄養学  [招待講演]
    上田宏一郎
    第3回天北放牧ネットテクニカルセミナー 2008年09月 口頭発表(基調)

その他活動・業績

受賞

  • 2001年03月 日本畜産学会 日本畜産学会奨励賞
     
    受賞者: 上田 宏一郎

共同研究・競争的資金等の研究課題

  • 群飼育下の乳用雌哺育牛から体調不良個体を早期発見するリアルタイムモニタリング技術の開発
    国立研究開発法人農業・食品産業技術総合研究機構 生物系特性産業技術センター:イノベーション創出強化研究推進事業
    研究期間 : 2020年05月 -2023年03月
  • 乳牛預託哺育・育成牧場の飼養管理実態調査事業
    日本中央競馬会:畜産振興事業
    研究期間 : 2019年04月 -2022年03月 
    代表者 : 上田 宏一郎
  • ワイン搾汁残渣タンニンを利用したラム肉パストラルフレーバー低減技術の確立
    公益財団法人伊藤記念財団:平成30年度公益財団法人伊藤記念財団助成
    研究期間 : 2018年06月 -2020年03月 
    代表者 : 上田 宏一郎
  • 集約放牧酪農経営における搾乳ロボット導入時の技術的課題の明確化
    農畜産業振興機構:平成29年度畜産関係学術研究委託調査
    研究期間 : 2017年06月 -2018年03月 
    代表者 : 上田 宏一郎
  • 放牧牛の採食動作の生体音響解析による牧草採食量および栄養価の推定
    科学技術振興機構:科学研究費補助金 萌芽研究
    研究期間 : 2005年04月 -2007年03月 
    代表者 : 上田 宏一郎
  • 乳牛の反芻胃内容物の消失における給与飼料間の相互作用に関する研究
    栗林育英学術財団:研究助成(個人研究)
    研究期間 : 1998年04月 -1999年03月 
    代表者 : 上田 宏一郎
  • Study on Dynamics of Ruminal Digesta in Ruminants

教育活動情報

主要な担当授業

  • 特別講義
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 国際
  • 畜牧体系学特論
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 農学院
    キーワード : 放牧、環境と生態系、家畜の消化管機能、栄養制御、飼養管理、行動、草地
  • 畜牧体系学特論演習
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 農学院
    キーワード : 放牧、環境と生態系、家畜の消化管機能、栄養制御、飼養管理、行動、草地
  • 大学院共通授業科目(一般科目):自然科学・応用科学
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 大学院共通科目
    キーワード : 育種、繁殖、遺伝子、飼養、管理、技術体系化、栄養、機能、胃腸管および周辺環境 Genetics, Breeding, Feeding, Management, Production System, Nutrition, Function, Gut and Surrounding environments
  • 家畜生産システム学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 乳肉生産、システム、環境、家畜飼養標準
  • 家畜生産学Ⅰ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 乳牛、飼養管理、飼料
  • 家畜生産論Ⅰ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 農学部
    キーワード : 乳牛、飼養管理、飼料
  • 家畜生産論Ⅱ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 農学部
    キーワード : 飼養管理,鶏、肉牛、豚、馬
  • 畜牧体系学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 農学部
    キーワード : 乳肉生産、システム、環境、家畜飼養標準
  • 畜牧体系学実験
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 農学部
    キーワード : 飼料成分、エネルギー利用、タンパク質利用、栄養価、乳牛
  • 家畜生産実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 農学部
    キーワード : 飼養管理、乳牛、豚、めん羊、鶏
  • 北海道農業概論
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 農学部
    キーワード : 自然生態、気象、作物、園芸作物、家畜、農業機械、農業経済 Natural Ecosystem, Climate, Field Crops, Horticultural Crops, Livestock, Agriculture Machinery, Agriculture Economy
  • 国際交流Ⅱ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 国際本部
    キーワード : 自然生態、気象、作物、園芸作物、家畜、農業機械、農業経済 Natural Ecosystem, Climate, Field Crops, Horticultural Crops, Livestock, Agriculture Machinery, Agriculture Economy
  • 一般教育演習(フレッシュマンセミナー)
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 全学教育
    キーワード : 生態系、家畜、畜産物、衣食住、文化

大学運営

委員歴

  • 2018年04月 - 現在   国立研究開発法人 農業・食品産業技術総合研究機構   家畜飼養標準等検討委員会委員
  • 2017年04月 - 現在   日本畜産学会   学会報編集委員
  • 2017年04月 - 現在   日本中央競馬会   競走馬総合研究所外部評価委員会
  • 2015年04月 - 現在   日本綿羊研究会   幹事
  • 2013年04月 - 現在   日本草地学会   評議員
  • 2012年04月 - 2014年03月   北海道畜産草地学会   庶務幹事
  • 2010年04月 - 2012年12月   北海道家畜管理研究会   庶務幹事
  • 2010年04月 - 2012年12月   北海道草地研究会   編集幹事
  • 2005年04月 - 2007年03月   北海道畜産学会   庶務幹事
  • 2000年04月 - 2001年03月   北海道草地研究会   会計幹事

社会貢献活動

  • 放牧酪農環境総合スマート管理サービス専門家指導員
    期間 : 2017年08月21日 - 2018年10月30日
    役割 : 助言・指導
    主催者・発行元 : ファームエイジ
  • 競走馬総合研究所外部評価委員会
    期間 : 2017年06月01日 - 2018年05月31日
    役割 : 助言・指導
  • 第34回グラスファーミングスクール
    期間 : 2017年05月16日 - 2017年05月18日
    役割 : 講師
  • 第27回グラスファーミングスクール
    期間 : 2014年05月27日
    役割 : 助言・指導
    主催者・発行元 : 創地農業21
  • 第26回グラスファーミングスクール
    期間 : 2013年04月13日 - 2013年04月15日
    役割 : 講師
    主催者・発行元 : 創地農業21


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