研究者データベース

笹岡 一慶(ササオカ カズヨシ)
獣医学研究院 附属動物病院
特任助教

基本情報

所属

  • 獣医学研究院 附属動物病院

職名

  • 特任助教

学位

  • 博士(獣医学)(2022年06月 北海道大学)

J-Global ID

研究キーワード

  • 神経超音波   脳神経内科   犬   画像診断   臨床獣医学   

研究分野

  • ライフサイエンス / 獣医学 / 臨床獣医学
  • ライフサイエンス / 獣医学 / 臨床獣医神経病学
  • ライフサイエンス / 獣医学 / 臨床獣医学

職歴

  • 2019年04月 - 現在 北海道大学大学院 附属動物病院 特任助教
  • 2018年04月 - 2019年03月 北海道大学大学院 獣医学研究科 日本学術振興会 特別研究員DC2
  • 2013年04月 - 2016年03月 田口動物病院 勤務獣医師

学歴

  • 2016年04月 - 2019年03月   北海道大学大学院   獣医学研究科   獣医内科学教室
  • 2007年04月 - 2013年03月   北海道大学   獣医学部   獣医学科
  • 2004年04月 - 2007年03月   群馬県立高崎高等学校   普通科

所属学協会

  • 獣医学会   獣医神経病学会   

研究活動情報

論文

  • Noriyuki Nagata, Hiryu Sawamura, Keitaro Morishita, Kenji Hosoya, Nozomu Yokoyama, Kazuyoshi Sasaoka, Noboru Sasaki, Kensuke Nakamura, Yoshinori Ikenaka, Mitsuyoshi Takiguchi
    The Journal of veterinary medical science 2022年05月27日 
    The urinary corticoid to creatinine ratio (UCCR) is one of the most commonly used screening tests for canine hypercortisolism (HC). In this study, a reference interval was established for UCCR using IMMULITE 2000 XPi, the latest chemiluminescence enzyme immunoassay. The diagnostic performance of this method for UCCR in canine HC was also evaluated. The median UCCR was 1.06 × 10-5 (range: 0.28-2.49) for 58 healthy dogs, and an upper reference limit of 1.98 × 10-5 (90% confidence interval: 1.76-2.15) was determined. The median UCCR in the 12 dogs with HC (7.38 × 10-5, range 1.86-29.98) was significantly higher than that in the 16 dogs with mimic-HC (1.59 × 10-5, range 0.47-3.42, P<0.001). The area under the curve for UCCR to differentiate HC dogs from mimic-HC dogs was 0.971, with a sensitivity of 91.7% and specificity of 100% when the cut-off value was set at 3.77 × 10-5. The UCCR of 16 paired urine samples collected at home and in hospital showed that the UCCR of samples collected in the hospital was significantly higher than that of samples collected at home (mean difference 3.30 × 10-5, 95% confidence interval: 0.70-5.90, P=0.001). In summary, we established the upper reference limit for UCCR using IMMULITE 2000 XPi in dogs and confirmed that UCCR is a useful diagnostic test for HC in dogs if urine samples are collected at home.
  • Kazuyoshi Sasaoka, Hiroshi Ohta, Tomohito Ishizuka, Kazuki Kojima, Noboru Sasaki, Mitsuyoshi Takiguchi
    American Journal of Veterinary Research 83 4 331 - 338 2022年04月 [査読有り]
     
    Abstract OBJECTIVE To investigate the association between changes in cerebral blood flow and electrographic epileptic seizure in dogs using transcranial Doppler ultrasonography (TCD). ANIMALS 6 healthy Beagle dogs. PROCEDURES Each dog was administered pentetrazol (1.5 mg/kg/min) or saline (0.9% NaCl) solution under general anesthesia with continuous infusion of propofol. Both pentetrazol and saline solution were administered to all 6 dogs, with at least 28 days interval between the experiments. Blood flow waveforms in the middle cerebral artery and the basilar artery were obtained using TCD at baseline, after pentetrazol administration, and after diazepam administration. TCD velocities, including peak systolic velocity, end-diastolic velocity, and mean velocity and resistance variables, were determined from the Doppler waveforms. RESULTS During ictal-phase of pentetrazol-induced seizures, the TCD velocities significantly increased in the basilar and middle cerebral arteries while TCD vascular resistance variables did not change in either artery. The TCD velocities significantly decreased after diazepam administration. Systemic parameters, such as the heart rate, mean arterial pressure, systemic vascular resistance, cardiac index, end-tidal carbon dioxide, oxygen saturation, and body temperature, did not change significantly during seizures. CLINICAL RELEVANCE This study showed that cerebral blood flow, as obtained from TCD velocities, increased by 130% during ictal-phase of pentetrazol-induced seizures in dogs. The elevated velocities returned to baseline after seizure suppression. Thus, TCD may be used to detect electrographic seizures during the treatment of status epilepticus in dogs, and further clinical studies clarifying the association between changes in cerebral blood flow and non-convulsive seizure cases are needed.
  • Dai HASEGAWA, Keisuke AOSHIMA, Kazuyoshi SASAOKA, Atsushi KOBAYASHI, Mitsuyoshi TAKIGUCHI, Takashi KIMURA
    Journal of Veterinary Medical Science 2022年
  • Noboru Sasaki, Thandar Oo, Yoshikazu Yasuda, Takahiro Ichise, Noriyuki Nagata, Nozomu Yokoyama, Kazuyoshi Sasaoka, Keitaro Morishita, Shouta Mm Nakayama, Mayumi Ishizuka, Kensuke Nakamura, Mitsuyoshi Takiguchi, Yoshinori Ikenaka
    The Journal of veterinary medical science 2021年09月17日 [査読有り]
     
    We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2-16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.
  • Yoshiki Ichikawa, Mizuki Heishima, Kristin Vyhnal, Keisuke Aoshima, Kazuyoshi Sasaoka, Ryohei Kinoshita, Atsushi Kobayashi, Mitsuyoshi Takiguchi, Takashi Kimura
    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 10406387211042587 - 10406387211042587 2021年09月04日 [査読有り]
     
    A 27-mo-old, spayed female mixed-breed dog was presented with left forelimb pain, which progressed to full thickness necrosis of the soft tissues of multiple limbs. Clinical imaging and postmortem examination suggested multiple large arterial thromboemboli. Histologic examination of vascular lesions revealed markedly thickened tunica intima with polypoid intraluminal projections, which partially to entirely occluded the arterial lumen. The expanded tunica intima was comprised of intimal accumulation of Alcian blue-positive matrix with scattered spindle-to-satellite cells. These cells were positive for von Willebrand factor and vimentin but negative for α-smooth muscle actin, suggesting endothelial origin. Deposition of the intimal mucoid matrix was observed in the elastic and muscular arteries associated with regional ischemic changes. Mucoid emboli, likely from fragmentation of proliferative intimal tissue, were identified in smaller vessels supplied by affected arteries. Based on these findings, we diagnosed systemic mucoid degeneration of the arterial tunica intima. Such systemic arterial degeneration characterized by deposition of mucoid matrix in the tunica intima has not been reported previously in dogs, to our knowledge, and should be distinguished from thromboembolism and other degenerative vascular diseases.
  • Tomohiro Suzuki, Anri Hirai, Kraisiri Khidkhan, Collins Nimako, Takahiro Ichise, Kazuki Takeda, Hazuki Mizukawa, Shouta M.M. Nakayama, Kei Nomiyama, Nobuhiko Hoshi, Mizuki Maeda, Tetsushi Hirano, Kazuyoshi Sasaoka, Noboru Sasaki, Mitsuyoshi Takiguchi, Mayumi Ishizuka, Yoshinori Ikenaka
    Pesticide Biochemistry and Physiology 175 104847 - 104847 2021年06月 [査読有り][通常論文]
     
    Fipronil is a phenylpyrazole insecticide that is widely used as a pesticide and a veterinary drug, although studies suggest that it could be toxic to mammals. The objectives of this study were to examine the pharmacokinetic profile of fipronil in mice, dogs, and cats, and to evaluate its effects on emotional and cognitive behaviors of dogs and cats using the data obtained from mice. The assessment of in vivo kinetics of fipronil was conducted in mice and dogs. We also performed behavioral tests (elevated plus-maze and Y-maze) and measured the levels of neurotransmitters in mice exposed to fipronil. In addition, the in vitro metabolism of fipronil were evaluated using liver microsomes of rats, mice, dogs, and cats. The results revealed that fipronil is distributed throughout the body (blood, brain, adipose tissue, and liver) of mice after dermal application. It was metabolized to fipronil sulfone primarily in the liver. The data on kinetics show that both fipronil and fipronil sulfone have a longer half-life in dogs and cats than in mice. The behavioral tests indicated that fipronil and fipronil sulfone could affect emotional and cognitive behaviors and alter the levels of neurotransmitters (dopamine in the striatum and serotonin in the hippocampus) in mice. Furthermore, we found that dogs and cats have a low ability to metabolize fipronil than mice and rats. However, further comprehensive studies are needed to determine whether fipronil affects the emotional and cognitive behaviors when administered to dogs and cats. To the best of our knowledge, this is the first study to examine the pharmacokinetic data and verify the effects of fipronil on emotional and cognitive behaviors of dogs and cats using the data obtained from mice.
  • T. Morita, K. Nakamura, T. Osuga, S. Kawamoto, S. Miki, K. Sasaoka, M. Takiguchi
    Journal of Veterinary Cardiology 32 16 - 27 2020年12月 [査読有り]
     
    © 2020 Elsevier B.V. Introduction: Pulmonary hypertension (PH) is a progressive and potentially life-threatening disease. Several drugs are used for the treatment of dogs with precapillary PH. Pimobendan is an inotropic drug with phosphodiesterase 3 inhibitory and calcium-sensitizing effects. Pimobendan administration improved right ventricular (RV) function and lowered pulmonary arterial pressure in some human patients with precapillary PH. However, the efficacy of pimobendan in dogs with precapillary PH is unknown. Animals, material, and methods: An implantable port device was percutaneously placed in the cranial vena cava of five laboratory beagles. Chronic embolic precapillary PH was induced via the repeated injection of microspheres every 1–2 days. Microsphere injection was continued until systolic pulmonary arterial pressure reached 50 mmHg. Right heart catheterization and echocardiography were performed at baseline and after injections of placebo and pimobendan (0.15 mg/kg). Results: Repeated injections of microspheres caused an increase in pulmonary vascular resistance, a decrease in stroke volume, RV dilation, left ventricular (LV) and RV dysfunction, and RV dyssynchrony as assessed using echocardiography. Compared with placebo, pimobendan improved LV and RV function based on the LV Tei index from 0.48 to 0.38 (p=0.002) and the RV Tei index from 0.76 to 0.61 (p=0.008), as well as the stroke volume index from 29.4 to 36.7 ml/m2 (p=0.012), respectively. Conclusions: In dog models of chronic PH, intravenous pimobendan effectively improved RV and LV function and increased stroke volume. However, pimobendan administration did not decrease pulmonary arterial pressure or produce hypotension.
  • Noboru Sasaki, Noriyuki Nagata, Keitaro Morishita, Tatsuyuki Osuga, Kazuyoshi Sasaoka, Nozomu Yokoyama, Hiroshi Ohta, Mitsuyoshi Takiguchi
    The Journal of veterinary medical science 82 7 1012 - 1016 2020年07月31日 [査読有り][通常論文]
     
    The feasibility of ultrasonographic measurement of thyroid gland area to common carotid artery (TG:CCA) was investigated. Twenty-one healthy, 12 hypothyroid and 18 non-thyroid illness (NTI) dogs were evaluated. The area of thyroid lobe and common carotid artery in right and left sides were measured using the same ultrasonographic images in transverse plane. The average of the right and left ratio was calculated as TG:CCA. The median TG:CCA of 21 healthy dogs was 1.53, and it did not correlate either body weight or age. The median TG:CCA of 12 hypothyroid dogs was 0.81, which was significantly lower than that of 18 NTI dogs (1.81, P<0.001). If the cut off value <1.12 was used, TG:CCA indicated hypothyroidism with a sensitivity of 100%, specificity of 83%, and accuracy of 90%. Our data indicated that TG:CCA was independent of both body weight, which may contribute to consistent measurement of thyroid size. The results of this study suggest that TG:CCA is a promising tool for diagnosing canine hypothyroidism.
  • Nisa K, Ohta H, Yokoyama N, Tamura M, Nagata N, Sasaoka K, Dermlim A, Leela-Arporn R, Morita T, Osuga T, Sasaki N, Morishita K, Nakamura K, Kagawa Y, Takiguchi M
    The Japanese journal of veterinary research 67 3 221 - 227 2019年08月 [査読有り][通常論文]
     
    Ultra low fat diet (ULFD) has shown a good therapeutic effect in dogs with intestinal lymphangiectasia secondary to CE (IL-CE). As we hypothesized that the mechanism of ULFD may involve the resolution of immunological impairment in IL-CE dogs, our study aimed to investigate the effect of ULFD on the circulating regulatory T cell (Treg) of IL-CE dogs. Treg frequency of pre- and post-ULFD blood samples from dogs with IL-CE (n = 3) were measured using flow cytometry. Treg frequency in IL-CE group before ULFD treatment showed lower tendency when compared to healthy control (mean +/- SD; 2.0 +/- 1.6% and 4.3 +/- 1.4%, respectively), and showed increased tendency after ULFD (mean +/- SD; 4.5 +/- 3.5%). The results suggested that ULFD might restore circulating Treg frequency of IL-CE dogs.
  • Angkhana Dermlim, Tatsuyuki Osuga, Kensuke Nakamura, Tomoya Morita, Khoirun Nisa, Kazuyoshi Sasaoka, Rommaneeya Leela-Arporn, Noriyuki Nagata, Masahiro Tamura, Noboru Sasaki, Hiroshi Ohta, Mitsuyoshi Takiguchi
    The Journal of veterinary medical science 81 7 949 - 957 2019年07月11日 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the cardiac acute volume loading effect on left atrial (LA) strain and strain rate (SR) parameters derived from two-dimensional speckle tracking echocardiography (2D-STE) in healthy dogs. Six healthy beagles were anesthetized and subjected to increase cardiac preload by intravenous infusion with lactated Ringer solution at 150 ml/kg/hr for 90 min. A Swan-Ganz catheter was placed to directly measure the mean pulmonary capillary wedge pressure (PCWP). Echocardiography was performed before (baseline) and at 15, 30, 45, 60, 75, and 90 min after acute volume loading began. Apical 4-chamber images focused on the LA were digitally recorded for later strain and SR analysis via 2D-STE. Acute volume loading significantly increased from baseline during LA strain and SR as assessed by the speckle tracking-based technique during reservoir and conduit function at 15 to 90 min after volume load began, and strain indices representing booster pump function were enhanced at 45 to 90 min. In addition, acute volume loading resulted in a significantly greater PCWP after fluid infusion. On multiple regression analysis, quadratic regression analysis was a better fit for the relationship between PCWP and all LA functional indices. Our findings indicated that LA function analyzed by strain and SR was enhanced during cardiac acute volume loading in healthy dogs. The change in strain and SR during acute volume loading should be interpreted with caution during the diagnosis of heart diseases related to volume overload.
  • Rommaneeya Leela-Arporn, Hiroshi Ohta, Masahiro Tamura, Noriyuki Nagata, Kazuyoshi Sasaoka, Angkhana Dermlim, Khoirun Nisa, Tatsuyuki Osuga, Keitaro Morishita, Noboru Sasaki, Mitsuyoshi Takiguchi
    Journal of veterinary internal medicine 33 4 1653 - 1659 2019年07月 [査読有り][通常論文]
     
    BACKGROUND: Metabolomic analysis using blood samples has been suggested to be useful for the early detection of cancer. Among metabolites, plasma-free amino acid (PFAA) profiles are potential diagnostic biomarkers for several diseases including cancer. However, the relationship between PFAA concentrations and liver tumors in dogs remains unknown. OBJECTIVE: To determine the characteristics of PFAA profiles of dogs with hepatocellular carcinoma (HCC) and correlated clinical features. ANIMALS: Thirty-four client-owned dogs diagnosed with HCC (n = 26) and benign liver diseases (n = 8) and 11 age-matched healthy dogs. METHODS: Prospective study using heparinized blood samples from fasted dogs. Plasma was deproteinized, and the concentrations of 21 amino acids were measured using an automated high-performance liquid chromatography amino acid analyzer. RESULTS: Plasma glutamic acid concentrations were significantly different among groups (P < .0024 after Bonferroni correction). Compared to healthy dogs, dogs with HCC and benign liver diseases had significantly higher concentrations of glutamic acid by post hoc analysis. However, no significant difference in the PFAA profiles of HCC and benign liver diseases were detected. In addition, preoperative and postoperative PFAA profiles of dogs with HCC were not significantly different. CONCLUSIONS AND CLINICAL IMPORTANCE: Increased glutamic acid concentrations might play a role in the development or be a consequence of liver tumor formation. However, PFAA profiles of HCC could not be differentiated from those of benign lesions. In addition, glutamic acid concentrations did not change after surgical resection. These results indicate that PFAA profiles may not be useful biomarkers for detecting HCC in dogs.
  • Leela-Arporn R, Ohta H, Nagata N, Sasaoka K, Tamura M, Dermlim A, Nisa K, Morishita K, Sasaki N, Nakamura K, Takagi S, Hosoya K, Takiguchi M
    Veterinary journal (London, England : 1997) 248 74 - 78 2019年06月 [査読有り][通常論文]
  • Dermlim A, Nakamura K, Morita T, Osuga T, Nisa K, Sasaoka K, Leela-arporn R, Sasaki N, Ohta H, Takiguchi M
    Journal of Veterinary Cardiology 23 69 - 80 2019年06月 [査読有り][通常論文]
  • Rommaneeya Leela-Arporn, Hiroshi Ohta, Masahiro Tamura, Noriyuki Nagata, Kazuyoshi Sasaoka, Angkhana Dermlim, Khoirun Nisa, Keitaro Morishita, Noboru Sasaki, Kensuke Nakamura, Satoshi Takagi, Kenji Hosoya, Mitsuyoshi Takiguchi
    The Journal of veterinary medical science 81 5 723 - 729 2019年05月31日 [査読有り][通常論文]
     
    A definitive diagnosis of focal liver lesions (FLLs) requires invasive procedures for histopathologic examination. Thus, a simpler noninvasive diagnostic method, such as conventional ultrasonography combined with clinical data, is needed for the prediction of liver malignancy. The objective of this study was to examine the diagnostic accuracy of clinical data and ultrasonographic (US) features to differentiate benign and malignant liver lesions. Medical records and US images from dogs with FLLs that underwent abdominal US and histopathologic examinations following surgery or liver biopsy were retrospectively reviewed. Clinical data, including signalment, clinical signs and laboratory findings, and the US features of liver lesions that could act as predictive factors were assessed using univariate and multivariate analyses to evaluate the associations between predictive factors and liver malignancy. Based on the histopathologic results, 55 dogs with malignant lesions and 28 dogs with benign lesions were included in the study. The results of univariate analysis showed that several US features and platelet count were significantly associated with liver malignancy. Multivariate analysis revealed that the platelet count (thrombocytosis; odds ratio [OR]: 4.13, 95% confidence intervals [CI]: 1.81-9.41), lesion size (4.1 cm or greater; OR: 23.83, 95% CI: 3.74-151.95) and echotexture of FLLs (heterogenous; OR: 8.44; 95% CI: 1.37-51.91) were independent predictors for differentiating benign and malignant liver lesions, suggesting that a combination of clinical data and US findings of FLLs could predict liver malignancy in dogs.
  • Khoirun Nisa, Sue Yee Lim, Masayoshi Shinohara, Tatsuyuki Osuga, Nozomu Yokoyama, Masahiro Tamura, Noriyuki Nagata, Kazuyoshi Sasaoka, Angkhana Dermlim, Rommaneeya Leela-Arporn, Tomoya Morita, Noboru Sasaki, Keitaro Morishita, Kensuke Nakamura, Hiroshi Ohta, Mitsuyoshi Takiguchi
    Journal of veterinary internal medicine 33 2 559 - 568 2019年03月 [査読有り][通常論文]
     
    BACKGROUND: Contrast-enhanced ultrasonography (CEUS) can be used to evaluate intestinal perfusion in healthy dogs. It is helpful for diagnosing and monitoring inflammatory bowel disease in humans and could be useful for dogs with chronic intestinal diseases. OBJECTIVES: To examine duodenal perfusion in dogs with chronic inflammatory enteropathy (CIE) and intestinal lymphoma. ANIMALS: Client-owned dogs with CIE (n = 26) or intestinal lymphoma (n = 7) and dogs with gastrointestinal signs but histopathologically normal duodenum (controls, n = 14). METHODS: In this cross-sectional study, dogs with CIE were classified into remission (n = 16) and symptomatic (n = 10) groups based on clinical scores determined at the time of CEUS. The duodenum was scanned after IV injection of Sonazoid® (0.01 mL/kg). CEUS-derived perfusion parameters, including time-to-peak, peak intensity (PI), area under the curve (AUC), and wash-in and wash-out rates were evaluated. RESULTS: The PI was significantly higher in the symptomatic CIE group (median (range); 105.4 (89.3-128.8) MPV) than in the control group (89.9 (68.5-112.2) MPV). The AUC was significantly higher in the symptomatic CIE group (4847.9 (3824.3-8462.8) MPV.sec) than in the control (3448.9 (1559.5-4736.9) MPV.sec) and remission CIE (3862.3 (2094.5-6899.0) MPV.sec) groups. The PI and clinical score were positively correlated in the CIE group. No significant differences in perfusion parameters were detected between the lymphoma and CIE groups or the lymphoma and control groups. CONCLUSIONS AND CLINICAL IMPORTANCE: The PI and AUC can detect duodenal inflammation and hence are potentially useful for excluding a diagnosis of CIE.
  • Khoirun Nisa, Sue Yee Lim, Tatsuyuki Osuga, Nozomu Yokoyama, Masahiro Tamura, Noriyuki Nagata, Kazuyoshi Sasaoka, Angkhana Dermlim, Rommaneeya Leela-Arporn, Tomoya Morita, Noboru Sasaki, Keitaro Morishita, Kensuke Nakamura, Hiroshi Ohta, Mitsuyoshi Takiguchi
    The Journal of veterinary medical science 80 3 453 - 459 2018年03月24日 [査読有り][通常論文]
     
    Quantitative contrast-enhanced ultrasonography (CEUS) enables non-invasive and objective evaluation of intestinal perfusion by quantifying the intensity of enhancement on the intestine after microbubble contrast administration. During CEUS scanning, sedation is sometimes necessary to maintain animal cooperation. Nevertheless, the effect of sedative administration on the canine intestinal CEUS is unknown. This study aimed to investigate the effect of sedation with a combination of butorphanol and midazolam on the duodenal CEUS-derived perfusion parameters of healthy dogs. For this purpose, duodenum was imaged following contrast administration (Sonazoid®, 0.01 ml/kg) in six healthy beagles before and after intravenous injection of a combination of butorphanol (0.2 mg/kg) and midazolam (0.1 mg/kg). Furthermore, hemodynamic parameters including blood pressure and heart rate were recorded during the procedure. Five CEUS derived perfusion parameters including time-to-peak (TTP), peak intensity (PI), area under the curve (AUC), wash-in and wash-out rates (WiR and WoR, respectively) before and after sedation were statistically compared. The result showed that no significant change was detected in any of perfusion parameters. Systolic and mean arterial pressures significantly reduced after sedative administration, but diastolic arterial pressure and heart rate did not significantly change. Moreover, no significant partial correlation was observed between perfusion parameters and hemodynamic parameters. Thus, we concluded that the combination did not cause significant influence in duodenal CEUS perfusion parameters and could be a good option for sedation prior to duodenal CEUS in debilitated dogs.
  • K. Sasaoka, K. Nakamura, T. Osuga, T. Morita, N. Yokoyama, K. Morishita, N. Sasaki, H. Ohta, M. Takiguchi
    Journal of Veterinary Internal Medicine 32 1 314 - 323 2018年01月01日 [査読有り][通常論文]
     
    Background: Transcranial Doppler ultrasound examination (TCD) is a rapid, noninvasive technique used to evaluate cerebral blood flow and is useful for the detection of intracranial hypertension in humans. However, the clinical usefulness of TCD in diagnosing intracranial hypertension has not been demonstrated for intracranial diseases in dogs. Objectives: To determine the association between the TCD variables and intracranial hypertension in dogs with intracranial diseases. Animals: Fifty client-owned dogs with neurologic signs. Methods: Cross-sectional study. All dogs underwent TCD of the basilar artery under isoflurane anesthesia after magnetic resonance imaging (MRI). Dogs were classified into 3 groups based on MRI findings: no structural diseases (group I), structural disease without MRI evidence of intracranial hypertension (group II), and structural disease with MRI evidence of intracranial hypertension (group III). The TCD vascular resistance variables (resistive index [RI], pulsatility index [PI], and the ratio of systolic to diastolic mean velocity [Sm/Dm]) were measured. Results: Fifteen, 22, and 13 dogs were classified into groups I, II, and III, respectively. Dogs in group III had significantly higher Sm/Dm (median, 1.78 range, 1.44–2.58) than those in group I (median, 1.63 range, 1.43–1.75) and group II (median, 1.62 range, 1.27–2.10). No significant differences in RI and PI were identified among groups. Conclusions and Clinical Importance: Our findings suggest that increased Sm/Dm is associated with MRI findings of suspected intracranial hypertension in dogs with intracranial diseases and that TCD could be a useful tool to help to diagnose intracranial hypertension.
  • Khoirun Nisa, Sue Yee Lim, Masayoshi Shinohara, Noriyuki Nagata, Kazuyoshi Sasaoka, Angkhana Dermlim, Rommaneeya Leela-Arporn, Tomoya Morita, Nozomu Yokoyama, Tatsuyuki Osuga, Noboru Sasaki, Keitaro Morishita, Kensuke Nakamura, Hiroshi Ohta, Mitsuyoshi Takiguchi
    The Journal of veterinary medical science 79 9 1585 - 1590 2017年09月29日 [査読有り][通常論文]
     
    Contrast-enhanced ultrasonography (CEUS) with microbubbles as a contrast agent allows the visualization and quantification of tissue perfusion. The assessment of canine intestinal perfusion by quantitative CEUS may provide valuable information for diagnosing and monitoring chronic intestinal disorders. This study aimed to assess the repeatability (intraday variability) and reproducibility (interday variability) of quantitative duodenal CEUS in healthy dogs. Six healthy beagles underwent CEUS three times within one day (4-hr intervals) and on two different days (1-week interval). All dogs were sedated with a combination of butorphanol (0.2 mg/kg) and midazolam (0.1 mg/kg) prior to CEUS. The contrast agent (Sonazoid®) was administered using the intravenous bolus method (0.01 ml/kg) for imaging of the duodenum. Time-intensity curves (TIC) were created by drawing multiple regions of interest (ROIs) in the duodenal mucosa, and perfusion parameters, including the time-to-peak (TTP), peak intensity (PI), area under the curve (AUC), and wash-in and wash-out rates (WiR and WoR, respectively), were generated. Intraday and interday coefficients of variation (CVs) for TTP, PI, AUC, WiR and WoR were <25% (range, 2.27-23.41%), which indicated that CEUS was feasible for assessing duodenal perfusion in healthy sedated dogs. A further study of CEUS in dogs with chronic intestinal disorders is necessary to evaluate its clinical applicability.
  • Yoshida T, Ichikawa N, Koike M, Hirokawa T, Sasaoka K, Machida N, Taguchi M
    Journal of Animal Clinical Medicine 25 4 148 - 152 2016年09月 [査読有り][通常論文]
  • 猫伝染性腹膜炎ウイルスの関与が疑われた拡張型心筋症様疾患の猫2例
    吉田 智彦, 市川 直紀, 小池 仁彦, 笹岡 一慶, 町田 登, 田口 正行
    動物臨床医学会年次大会プロシーディング 36回 2 33 - 34 動物臨床医学会 2015年11月
  • Clinical usefulness of Transcranial Doppler ultrasound in dogs.
    Kazuyoshi Sasaoka
    Japanese Journal of Veterinary Research 61 51 - 52 2013年 [査読無し][通常論文]

講演・口頭発表等

  • 鈴木智裕, 池中良徳, 中山翔太, 水川葉月, 一瀬貴大, 佐々木東, 笹岡一慶, 石塚真由美
    第162回日本獣医学会学術集会 2019年09月 口頭発表(一般)
  • 坂本暁彦, 大田寛, 好井健太朗, 矢吹悠久美, 笹岡一慶, 大菅辰幸, 佐々木東, 森下啓太郎, 滝口満喜
    第162回日本獣医学会学術集会 2019年09月 口頭発表(一般)
  • 井上裕美子, 佐々木東, 池中良徳, 一瀬貴大, 笹岡一慶, 大菅辰幸, 中山翔太, 森下啓太郎, 大田寛, 石塚真由美, 滝口満喜
    第162回日本獣医学会学術集会 2019年09月 口頭発表(一般)
  • 黒田杏子, 大菅辰幸, 笹岡一慶, 森下啓太郎, 佐々木東, 大田寛, 滝口満喜
    第162回日本獣医学会学術集会 2019年09月
  • 笹岡一慶, 森下啓太郎, 細谷謙次, 滝口満喜
    令和元年小動物獣医学会(北海道) 2019年08月 口頭発表(一般)
  • 新井田篤, 大田寛, 笹岡一慶, 細谷謙次, 市居修, 賀川由美子, 大菅辰幸, 佐々木東, 森下啓太郎, 滝口満喜
    令和元年小動物獣医学会(北海道) 2019年08月 口頭発表(一般)
  • 田中啓介, 野見山桂, 水川葉月, 水川葉月, 高口倖暉, 田上瑠美, 横山望, 市居修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介
    環境化学討論会要旨集(CD-ROM) 2019年06月
  • 経頭蓋超音波ドプラ法による脳血流評価を行った脳梗塞の犬の1例:脳動脈のダイナミックな血流変化の検出.  [通常講演]
    笹岡 一慶, 大田 寛, 石塚 友人, 大菅 辰幸, 森下 啓太郎, 佐々木 東, 滝口 満喜
    第15回日本獣医内科学アカデミー学術大会 2019年02月 口頭発表(一般) 横浜
  • 一般診療エリアにおける院内感染対策:手術部位感染(SSI)対策を極める!〜院内感染症対策と外科医にできる工夫〜  [招待講演]
    笹岡 一慶
    日本獣医麻酔外科学会北海道地区講習会 2019年02月 公開講演,セミナー,チュートリアル,講習,講義等
  • 笹岡一慶, 大田寛, 石塚友人, 大菅辰幸, 立花由莉加, 森下啓太郎, 佐々木東, 滝口満喜
    平成30年度小動物獣医学会(北海道) 2018年09月 口頭発表(一般) 札幌
  • 大村侑己, 大田寛, 小林篤史, 笹岡一慶, 道下明日香, 大菅辰幸, 佐々木東, 森下啓太郎, 滝口満喜
    平成30年度小動物獣医学会(北海道) 2018年09月 口頭発表(一般) 札幌
  • 山田有紗, 笹岡一慶, 大田寛, 立花由莉加, 大菅辰幸, 佐々木東, 森下啓太郎, 滝口満喜
    平成30年度小動物獣医学会(北海道) 2018年09月 口頭発表(一般) 札幌
  • Coming back to the graduate school.  [通常講演]
    Kazuyoshi SASAOKA
    The 6th Sapporo Summer Seminar for One Health (Sapporo) 2018年09月 シンポジウム・ワークショップパネル(指名) Sapporo
  • Effect of cardiac volume loading on left atrial longitudinal strain analysis derived from two-dimensional Speckle Tracking Echocardiography in healthy dogs.  [通常講演]
    Angkhana Dermlim, 中村 健介, 森田 智也, 大菅 辰幸, Khoirun Nisa, Rommaneeya Leela-arporn, 笹岡 一慶, 永田 矩之, 田村 昌大, 佐々木 東, 大田 寛, 森下 啓太郎, 滝口 満喜
    第161回日本獣医学会学術集会 2018年09月 口頭発表(一般) 筑波
  • 佐々木東, 笹岡一慶, 大田寛, 大菅辰幸, 森下啓太郎, 滝口満喜
    日本超音波医学会平成28年度第1回超音波分子診断治療研究会 2018年08月 口頭発表(一般) 札幌
  • Demographic Features, Characteristics and Risk Factors in a Retrospective Study of Hepatocellular Carcinoma in Dogs.  [通常講演]
    Rommaneeya Leela-arporn, Hiroshi Ohta, Noriyuki Nagata, Kazuyoshi Sasaoka, Tatsuyuki Osuga, Keitaro Morishita, Noboru Sasaki, Kensuke Nakamura, Mitsuyoshi Takiguchi
    American College of Veterinary Internal Medicine Forum 2018 2018年06月 ポスター発表 Seattle
  • Trace Elemental Profiles of Serum and Cerebrospinal Fluid in Dogs with Idiopathic Epilepsy.  [通常講演]
    Kazuyoshi Sasaoka, Hiroshi Ohta, Hazuki Mizukawa, Shouta Nakayama, Noboru Sasaki, Keitaro Morishita, Yoshinori Ikenaka, Mayumi Ishizuka, Mitsuyoshi Takiguchi
    American College of Veterinary Internal Medicine Forum 2018 2018年06月 ポスター発表 Seattle
  • てんかん重積状態への対処 ICU管理の1例から.  [通常講演]
    笹岡 一慶
    北海道大学附属動物病院卒後教育セミナー 2018年06月 公開講演,セミナー,チュートリアル,講習,講義等
  • 水川葉月, KHIDKHAN Kraisiri, 高口倖暉, 横山望, 市居修, 滝口満喜, 笹岡一慶, 池中良徳, 池中良徳, 中山翔太, TSEND‐AYUSH Sainnoxoi, 野見山桂, 田辺信介, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 那覇
  • 田中啓介, 野見山桂, 水川葉月, 高口倖暉, 田上瑠美, 横山望, 市居修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介
    第27回環境化学討論会 2018年05月 口頭発表(一般) 那覇
  • Risk factors for hepatocellular carcinoma in dogs: a retrospective case-control study.  [通常講演]
    Rommaneeya Leela-arporn, Hiroshi Ohta, Noriyuki Nagata, Masahiro Tamura, Kazuyoshi Sasaoka, Angkhana Dermlim, Khoirun Nisa, Tomoya Morita, Tatsuyuki Osuga, Morishita Keitaro, Noboru Sasaki, Mitsuyoshi Takiguchi
    第14回日本獣医内科学アカデミー学術大会 2018年02月 口頭発表(一般) 横浜
  • アドレナリン持続点滴が心原性肺水腫の救急管理に有用であった犬の1例.  [通常講演]
    小島 一輝, 石塚 友人, 笹岡 一慶, 大菅 辰幸, 中村 健介, 滝口 満喜
    第14回日本獣医内科学アカデミー学術大会 2018年02月 口頭発表(一般) 横浜
  • 犬の特発性てんかんにおける血中及び脳脊髄液中微量元素濃度の検討.  [通常講演]
    笹岡 一慶, 大田 寛, 水川 葉月, 中山 翔太, 佐々木 東, 森下 啓太郎, 池中 良徳, 石塚 真由美, 滝口 満喜
    第14回日本獣医内科学アカデミー学術大会 2018年02月 口頭発表(一般) 横浜
  • Relationship between transcranial Doppler ultrasound vascular resistance parameters and intracranial hypertension in clinical dogs.  [通常講演]
    Kazuyoshi SASAOKA, Hiroshi OHTA, Noboru SASAKI, Mitsuyoshi TAKIGUCHI
    Asian Meeting of Animal Medicine Specialties 2017 2017年11月 ポスター発表 Taegu
  • 超音波診断装置を用いた脳圧・発作の診断 −容易に利用できる診断ツールを小動物獣医療から人医療へ−  [通常講演]
    笹岡一慶
    博士課程教育リーディングフォーラム2017 2017年10月 ポスター発表 名古屋
  • Effects of Organohalogen Compounds on Serum Biochemical Profiles in Cats.  [通常講演]
    Kraisiri KHIDKHAN, Hazuki MIZUKAWA, Yoshimori IKENAKA, Shota M, M. NAKAYAMA, Kei NOMIYAMA, Nozomu YOKOYAMA, Kazuyoshi SASAOKA, Osamu ICHII, Mitsuyoshi TAKIGUCHI, Shinsuke TANABE, Mayumi ISHIZUKA
    The 5th Sapporo Summer Seminar for One Health 2017年09月 ポスター発表 Sapporo
  • Elevation of transcranial Doppler ultrasound vascular resistance parameters are associated with intracranial hypertension in dogs with brain diseases.  [通常講演]
    Kazuyoshi SASAOKA, Hiroshi OHTA, Noboru SASAKI, Mitsuyoshi TAKIGUCHI
    The 5th Sapporo Summer Seminar for One Health 2017年09月 ポスター発表 Sapporo
  • Quantitative contrast-enhanced ultrasonography of duodenum in dogs with chronic enteropathy.  [通常講演]
    Khoirun Nisa, 大田 寛, 横山 望, 田村 昌大, 永田 矩之, 笹岡 一慶, Angkhana Dermlim, Rommaneeya Leela-Arporn, 森田 智也, 大菅 辰幸, 佐々木 東, 森下 啓太郎, 滝口 満喜
    第160回日本獣医学会学術集会 2017年09月 口頭発表(一般) 鹿児島
  • 笹岡一慶, 大田寛, 田村昌大, 永田矩之, LEELA‐ARPORN Rommaneeya, DERMLIM Angkhana, KHOIRUN Nisa, 森田智也, 佐々木東, 森下啓太郎, 滝口満喜
    第160回日本獣医学会学術集会 2017年09月 口頭発表(一般) 鹿児島
  • 奥朋哉, 大田寛, 田村昌大, 永田矩之, 笹岡一慶, LEELA‐ARPORN Rommaneeya, DERMLIM Angkhana, NISA Khoirun, 森田智也, 大菅辰幸, 佐々木東, 森下啓太郎, 滝口満喜
    第160回日本獣医学会学術集会 2017年09月 口頭発表(一般) 鹿児島
  • 永田矩之, 大田寛, 横山望, 田村昌大, 笹岡一慶, LEELA‐ARPORN Rommaneeya, DERMLIM Angkhana, NISA Khoirun, 森田智也, 大菅辰幸, 佐々木東, 森下啓太郎, 滝口満喜
    第160回日本獣医学会学術集会 2017年09月 口頭発表(一般) 鹿児島
  • 水川葉月, 野見山桂, 池中良徳, 池中良徳, 中山翔太, SAENGTIENCHAI Aksorn, SAINNOXOI Tsendayush, 横山望, 笹岡一慶, 市居修, KHIDKHAN Kraisiri, 高居名菜, 滝口満喜, 田辺信介, 石塚真由美
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡
  • 犬における大脳白質病変の臨床的意義に関する検討: 高齢犬の側脳室周囲高信号は症状に関連するか?  [通常講演]
    笹岡 一慶, 大田 寛, 華園 究, 佐々木 東, 森下 啓太郎, 中村 健介, 滝口 満喜
    第13回日本獣医内科学アカデミー学術大会 2017年02月 口頭発表(一般) 横浜
  • 経頭蓋超音波ドプラ法を用いた犬の非侵襲的脳圧亢進診断.  [通常講演]
    笹岡 一慶, 大田 寛, 佐々木 東, 滝口 満喜
    北海道大学第3回部局横断シンポジウム 2017年01月 ポスター発表 札幌
  • 長谷川純也, 大田寛, 横山望, 笹岡一慶, NISA Khoirun, 森田智也, 大菅辰幸, 佐々木東, 森下啓太郎, 中村健介, 滝口満喜
    第159回日本獣医学会学術集会 2016年08月 口頭発表(一般) 藤沢
  • 吉田智彦, 市川直紀, 小池仁彦, 笹岡一慶, 町田登, 田口正行
    第36回動物臨床医学会 2015年11月 口頭発表(一般) 大阪
  • 吉田智彦, 市川直紀, 小池仁彦, 笹岡一慶, 町田登, 田口正行
    第35回動物臨床医学会 2014年11月 口頭発表(一般) 大阪
  • 消化管にクリプトコッカス病変が認められた猫の1例.  [通常講演]
    田口 正行, 市川 直紀, 小池 仁彦, 吉田 智彦, 笹岡 一慶
    第23回中部小動物臨床研究発表会 2014年10月 口頭発表(一般) 名古屋
  • 吉田智彦, 市川直紀, 小池仁彦, 笹岡一慶, 田口正行
    第34回動物臨床医学会 2013年11月 口頭発表(一般) 大阪
  • 笹岡一慶, 市川直紀, 小池仁彦, 吉田智彦, 田口正行
    第34回動物臨床医学会 2013年11月 口頭発表(一般) 大阪
  • 経頭蓋超音波ドプラ法の臨床的有用性に関する研究.  [通常講演]
    笹岡 一慶, 中村 健介, 森下 啓太郎, 大菅 辰幸, Lim Sue Yee, 横山 望, 大田 寛, 山_ 真大, 滝口 満喜
    第9回日本獣医内科学アカデミー学術大会 2013年02月 口頭発表(一般) 横浜
  • 笹岡一慶, 中村健介, 森下啓太郎, 大菅辰幸, LIM Sue Yee, 大田寛, 山崎真大, 滝口満喜
    第51回日本獣医画像診断学会 2012年06月 口頭発表(一般) 大宮

作品等

その他活動・業績

受賞

  • 2021年09月 日本獣医学会 2020年JVMS優秀論文賞
     An area ratio of thyroid gland to common carotid artery for evaluating the thyroid gland size. 
    受賞者: Sasaki N;Nagata N;Morishita K;Osuga T;Sasaoka K;Yokoyama N;Ohta H;Takiguchi M
  • 2021年09月 日本獣医学会 Good Reviewer賞(2020)
     
    受賞者: 笹岡一慶
  • 2018年03月 2016年度クリニカルニュートリション研究会スカラーシッププログラム 優秀研究アワード
     犬の特発性てんかんにおける血中および脳脊髄液中の微量元素濃度の検討 
    受賞者: 笹岡一慶
  • 2017年11月 Asian Meeting of Animal Medicine Specialties 2017 Best Poster Award
     Relationship between transcranial Doppler ultrasound vascular resistance parameters and intracranial hypertension in clinical dogs 
    受賞者: 笹岡一慶
  • 2013年02月 第9回日本獣医内科学アカデミー学術大会 学術奨励賞<臨床研究AWARD>
     犬の頭蓋内疾患診断における経頭蓋超音波ドプラ法の有用性の検討 
    受賞者: 笹岡一慶
  • 2008年07月 北海道大学 北海道大学新渡戸賞
     
    受賞者: 笹岡一慶

共同研究・競争的資金等の研究課題

  • 犬における臨床応用可能な脳血管反応性検査法の確立
    日本学術振興会:科学研究費助成事業 基盤研究(C)
    研究期間 : 2021年04月 -2024年03月 
    代表者 : 笹岡 一慶
  • 日本学術振興会:科学研究費助成事業 特別研究員奨励費
    研究期間 : 2018年04月 -2020年03月 
    代表者 : 笹岡 一慶
     
    獣医療において、重度なけいれん発作の治療モニタリングはほとんど行われてこなかった。脳波検査に基づく電気活動的な“Seizure Free”が治療の目標であるが、獣医療において現状利用可能な肉眼的観察では“Seizure Free”が達成されているかは分からない。本研究では近年獣医療で急速に普及している超音波診断装置を利用し、経頭蓋超音波ドプラ法による脳血流評価を用いたけいれん発作の新規治療モニタリング法を検討することとした。本研究では①実験的けいれん発作モデルにおける脳血流波形変化の解析、②集中治療中のけいれん発作症例犬における経時的な脳血流波形解析、のアプローチを計画した。 2018年度は実験的けいれん発作モデル犬における脳血流波形変化の解析を目標とし、実験的にけいれん発作モデルを作出し、発作によって生じる脳血流波形の変化を経頭蓋超音波ドプラ法を用いて解析した。全身麻酔下の正常実験犬にペンテトラゾール持続点滴により実験的けいれん発作モデル犬を作出した。本モデルにおける発作時には脳底動脈平均血流速度の120-160%上昇、中大脳動脈平均血流速度の120-220%上昇を認め、経頭蓋超音波ドプラ法において脳血流速度上昇が発作の指標となることが示唆された。また、脳血管抵抗指標は脳底動脈で低下傾向を認め、発作時において脳血管拡張が生じていることを経頭蓋超音波ドプラ法が捉えているものと考えられた。今後、血流波形の更なる解析や他の生理的パラメータとの組み合わせにより、さらに鋭敏に発作時の血流変化を捉える指標を検討し、臨床症例において蓄積されたデータを検討する予定である。また、同手法の研究を通じて、犬の脳梗塞症例における経時的な脳血流変化を獣医学領域で初めて捉えることに成功した。学会報告および国際誌への投稿を予定している。 今後も経頭蓋超音波ドプラ法の適応拡大を目標に研究を実施する。
  • 経頭蓋超音波ドプラ法による犬の脳血流評価:発作モニタリングへの応用の検討
    北海道大学博士課程教育リーディングプログラム「One Healthに貢献する獣医科学グローバルリーダー育成プログラム」:平成29年度リーディングプログラム大学院学生科学研究費
    研究期間 : 2017年08月 -2018年03月 
    代表者 : 笹岡一慶
  • 犬の特発性てんかんにおける血中および脳脊髄液中微量元素濃度の検討
    クリニカルニュートリション研究会:クリニカルニュートリション研究会2016年度スカラーシッププログラム
    研究期間 : 2016年06月 -2017年10月 
    代表者 : 笹岡一慶
  • 北海道大学博士課程教育リーディングプログラム「One Healthに貢献する獣医科学グローバルリーダー育成プログラム」:平成28年度リーディングプログラム大学院学生科学研究費
    研究期間 : 2016年08月 -2017年03月 
    代表者 : 笹岡一慶

教育活動情報

主要な担当授業

  • 総合専門臨床特論
    開講年度 : 2021年
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