研究者データベース

井上 哲(イノウエ サトシ)
歯学研究院
教授

基本情報

所属

  • 歯学研究院

職名

  • 教授

学位

  • 歯学博士(北海道大学)

J-Global ID

研究キーワード

  • 臨床的スミア層   1ステップ型接着システム   透過型電子顕微鏡   微小引張り接着強さ   簡略化ステップ型接着システム   微小引張り強さ   接着性能   サーマルサイクリング   機能性モノマー   走査型電子顕微鏡   細胞内情報伝達系   根尖病巣   機能生モノマー   歯学   簡略化ステップ接着システム   嫌気性菌   難治性感染根管   簡略化接着システム   ナノテクノロジー   歯質接着   接着システム   劣化   接着耐久性   耐久性   バイオデグラデーション   溶媒   接着界面   根管治療実態調査   Mycoplasma salivarium   コントラクションギャップ   歯科医学教育   歯科保存学   Conservative Dentistry   

研究分野

  • ライフサイエンス / 保存治療系歯学
  • ライフサイエンス / 常態系口腔科学
  • ライフサイエンス / 保存治療系歯学

職歴

  • 2013年05月 - 2013年 北海道大学 大学院歯学研究科 臨床教育部門 大学病院 口腔総合治療部 教授

学歴

  • 1982年04月 - 1986年03月   北海道大学   大学院 歯学研究科
  • 1976年04月 - 1982年03月   北海道大学   歯学部

所属学協会

  • 日本総合歯科学会   日本歯科医学教育学会   日本歯科理工学会   日本歯科保存学会   International Association for Dental Research   日本接着歯学会   日本歯科保存学会(理事)   Japanese Society for Adhesive Dentistry   International Association for Dental research   Japanese Society of Conservative Dentistry   

研究活動情報

論文

  • Anri Fukuoka, Kenichi Koshiro, Satoshi Inoue, Yasuhiro Yoshida, Toru Tanaka, Takatsumi Ikeda, Kazuomi Suzuki, Hidehiko Sano, Bart Van Meerbeek
    JOURNAL OF ADHESIVE DENTISTRY 13 3 243 - 248 2011年06月 [査読有り][通常論文]
     
    Purpose: To evaluate the hydrolytic stability of three one-step self-etching adhesives (1-SEAs) bonded to dentin through bond strength testing and ultra-morphological interfacial analysis before and after long-term thermocycling. Materials and Methods: Eighteen flattened mid-coronal dentin surfaces of extracted human molars were subjected to bonding treatment using three 1-SEAs (Clearfil S(3) Bond, Kuraray (S3), G-Bond, GC (GB), Absolute, Dentsply-Sankin (AB)), after which the bonded surfaces were built up with composite. After storage overnight at 37 C, the specimens were sectioned into slabs and further trimmed into an hourglass shape with an interface area of approximately 1 mm(2). The specimens were left untouched (control) or were thermocycled for 100,000 cycles. The microtensile bond strength (mu TBS) was measured and the ultrastructure of the adhesive/dentin interface characterized using transmission electron microscopy (TEM). Results: Long-term thermocycling significantly decreased the mu TBS of all one-step adhesives tested (p < 0.05, one-way ANOVA and Games-Howell test). TEM revealed a similar interfacial ultrastructure before and after thermocycling for S3. For GB, many voids were observed at the interface after 100,000 thermocycles. Regarding AB, collagen fibrils could no longer be clearly observed upon staining, while the adjacent unaffected dentin was rich in voids. Conclusion: The bond strength and ultramorphological data demonstrated that the bond of 1-SEAs to dentin degrades with time, although the degree of degradation is obviously material dependent.
  • S Inoue, K Koshiro, Y Yoshida, J De Munck, K Nagakane, K Suzuki, H Sano, B Van Meerbeek
    JOURNAL OF DENTAL RESEARCH 84 12 1160 - 1164 2005年12月 [査読有り][通常論文]
     
    Functional monomers chemically interact with hydroxyapatite that remains within submicron hybrid layers produced by mild self- etch adhesives. The functional monomer 10- MDP interacts most intensively with hydroxyapatite, and its calcium salt appeared most hydrolytically stable, as compared with 4- MET and phenyl- P. We investigated the hypothesis that additional chemical interaction of self- etch adhesives improves bond stability. The micro- tensile bond strength (mu TBS) of the 10- MDP- based adhesive did not decrease significantly after 100,000 cycles, but did after 50,000 and 30,000 cycles, respectively, for the 4- MET- based and the phenyl-P- based adhesives. Likewise, the interfacial ultrastructure was unchanged after 100,000 thermocycles for the 10- MDP- based adhesive, while that of both the 4- MET- and phenyl- P- based adhesives contained voids and less- defined collagen. The findings of this study support the concept that long- term durability of adhesive-dentin bonds depends on the chemical bonding potential of the functional monomer.
  • T Ikeda, J De Munck, K Shirai, K Hikita, S Inoue, H Sano, P Lambrechts, B Van Meerbeek
    DENTAL MATERIALS 21 11 1051 - 1058 2005年11月 [査読有り][通常論文]
     
    Objective. The purpose of the present study was to evaluate the effect of evaporation of primer components on the ultimate tensile strength (UTS) of primer-adhesive mixtures. Methods. The UTSs of 1:3 by weight primer-adhesive mixtures and of the pure adhesives (controls) were measured after 0-, 2-, 5-min and 24-h storage in a 37 degrees C oven for the three-step etchftrinse adhesive OptiBond FL (Kerr) and the two-step self-etch adhesive Ctearfil SE (Kuraray). Results. Evaporation of primer components increased with time for both adhesives investigated. OptiBond FL showed a higher evaporation of primer components than Ctearfil SE at the 2-, 5-min and 24-h storage time. As compared to the pure adhesive, the UTS of the primer-adhesive mixture was lower after 0- and 2-min storage for OptiBond FL and after 0-, 2- and 5-min storage for Clearfil SE. The UTS of OptiBond FL was higher than that of Clearfil SE at the 0- and 2-min storage time, though there was no difference at the 5-min and 24-h storage time. Significance. The degree of evaporation of primer components depended largely on the primer solvents employed. The significant decrease in UTS of adhesive resin when mixed with primer must be attributed to incomplete evaporation of primer components. Complete evaporation of solvents is hard to achieve, even by thorough air drying. (c) 2005 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • K Koshiro, S Inoue, H Sano, J De Munck, B Van Meerbeek
    EUROPEAN JOURNAL OF ORAL SCIENCES 113 4 341 - 348 2005年08月 [査読有り][通常論文]
     
    Resin-dentin bonds are known to degrade in the relatively aggressive oral environment. In order to obtain greater insight into the interfacial degradation process, we examined, by using transmission electron microscopy (TEM), the interfacial ultrastructure of two adhesives bonded to dentin after 1 yr in vivo. Class V cavities were prepared on the buccal surfaces of 14 intact teeth of two monkeys and then restored by using either the two-step self-etch adhesive, Unifil Bond, or the two-step etch-and-rinse adhesive, Single Bond, in combination with the restorative microhybrid composite, Z250. After 1 yr, 10 other teeth were restored by using the same materials (controls). One day later, the monkeys were killed, following which the microtensile bond strength to dentin was determined and the interfacial ultrastructure was examined by TEM. Whereas no noticeable changes in the morphology of the resin-dentin interface were observed between the 1-d and 1-yr specimens for Unifil Bond, Single Bond exhibited signs of interfacial degradation, in particular at the bottom of the 3 mu m-deep hybrid layer. In conclusion, the adhesive interface produced by the etch-and-rinse adhesive was less resistant to degradation than that produced by the self-etch system.
  • T Ikeda, J De Munck, K Shirai, K Hikita, S Inoue, H Sano, P Lambrechts, B Van Meerbeek
    DENTAL MATERIALS 21 5 413 - 420 2005年05月 [査読有り][通常論文]
     
    Objective. The purpose of this study was to measure the micro-tensile bond strength (JBS) of two adhesives to dentin and to correlate this JBS with the micro-tensile fracture strength (mu TFS) of primer-adhesive mixtures of the two adhesives. Methods. The three-step etchEtrinse adhesive OptiBond FL (Kerr) and the two-step self-etch adhesive Clearfil SE Bond (Kuraray) were used to bond Filtek Z100 (3M ESPE) to twenty mid-coronal dentin surfaces. The mu TBS was determined at 1 and 24 h after tight-curing. In addition, the mu TFS of primer-adhesive mixtures (primer: adhesive= 1:3 by weight) and of the pure adhesives (controls) were measured after the same time intervals. Results. The JBS to dentin of OptiBond FL at 24 h was significantly higher than at 1 h and than the mu TBS of Clearfil SE Bond at 24 h. The mean mu TFS of the primeradhesive mixtures was always lower than the mu TFS of the respective adhesives. The highest reduction in mu TFS due to mixing primer with the adhesive resin was measured for Clearfil. SE Bond. Both the mu TFS of the primer-adhesive mixtures and the pure adhesives of OptiBond FL and Clearfil SE Bond increased with time. Significance. The early 1 h mu TBS to dentin was lower than the 24 h mu TFS for OptiBond FL, but not for Clearfil SE Bond. Differences in polymerization efficiency as well as in the resulting interfacial strength may explain such varying early bond strengths to dentin. (c) 2004 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • S Inoue, Y Abe, Y Yoshida, J De Munck, H Sano, K Suzuki, P Lambrechts, B Van Meerbeek
    OPERATIVE DENTISTRY 29 6 685 - 692 2004年11月 [査読有り][通常論文]
     
    The effect of a polyalkenoic acid conditioner pretreatment on the bond strength of a glass-ionomer adhesive to tooth substrates with or without smear layer was evaluated. Smear-layer covered and smear-layer free dentin and enamel surfaces were prepared from 24 extracted human molars. Resin composite was bonded to the sur faces using FuJiBond LC with or without a polyalkenoic acid conditioner and subjected to microtensile bond strength (muTBS) testing. Failure modes were determined using scanning electron microscopy. For dentin, smear-layer coverage and conditioner treatment did not reveal significant differences in muTBS, which ranged from 20 to 29 MPa. For enamel, smear-layer coverage did not significantly affect muTBS, whereas, the use of conditioner significantly improved MTBS, reaching the same MTBS-values as when bonded to dentin. Regarding failure mode, most dentin specimens failed mixed adhesive cohesively. For enamel, adhesive failures mostly occurred when no conditioner was used, though mixed failures were predominant when the specimens were conditioned beforehand. Bonding of the glass ionomer adhesive to dentin can be achieved without the separate use of a polyalkenoic acid conditioner, even with the interposition of a smear layer. However, instrumented and non-instrumented enamel requires separate conditioning to provide sufficient micro-mechanical retention.
  • S Inoue, MA Vargas, Y Abe, Y Yoshida, P Lambrechts, G Vanherle, H Sano, B Van Meerbeek
    AMERICAN JOURNAL OF DENTISTRY 16 5 329 - 334 2003年10月 [査読有り][通常論文]
     
    Purpose: To compare the microtensile bond strength (muTBS) to enamel of 10 contemporary adhesives, including three one-step self-etch systems, four two-step self-etch systems and three two-step total-etch systems, with that of a conventional three-step total-etch adhesive. Methods: Resin composite (Z100, 3M) was bonded to flat, #600-grit wet-sanded enamel surfaces of 18 extracted human third molars using the adhesives strictly according to the respective manufacturer's instructions. After storage overnight in 37degreesC water, the bonded specimens were sectioned into 2-4 thin slabs of approximately 1 mm thickness and 2.5 mm width. They were then trimmed into an hourglass shape with an interface area of approximately 1 mm(2), and subsequently subjected to muTBS-testing with a cross-head speed of 1 mm/minute. Results: The muTBS to enamel varied from 3.2 MPa for the experimental one-step self-etch adhesive PQ/Universal (self-etch) to 43.9 MPa for the two-step total-etch adhesive Scotchbond 1. When compared with the conventional three-step total-etch adhesive OptiBond FL, the bond strengths of most adhesives with simplified application procedures were not significantly different, except for two one-step self-etch adhesives, experimental PQ/Universal (self-etch) and One-up Bond F, that showed lower bond strengths. Specimen failures during sample preparation were recorded for the latter adhesives as well.
  • R Fukuda, Y Yoshida, Y Nakayama, M Okazaki, S Inoue, H Sano, K Suzuki, H Shintani, B Van Meerbeek
    BIOMATERIALS 24 11 1861 - 1867 2003年05月 [査読有り][通常論文]
     
    Previously, we introduced a methodology to determine the chemical bonding potential of polyalkenoic acids to mineralized tissues through quantification of the degree of ionic bond formation between the carboxyl groups of a polyalkenoic acid with calcium of hydroxyapatite. In a continuation of that study, we now investigated in how far the chemical bonding potential to synthetic hydroxyapatite is influenced by the molecular structure of the polyalkenoic acid and if this also may affect the self-adhesiveness to enamel and dentin. X-ray photoelectron spectroscopy and inductively coupled plasma atomic emission spectrometry were used to quantitatively analyze the chemical bonding efficacy of a polyalkenoic acid consisting of acrylic acid units (PAA) to synthetic hydroxyapatite (HAp) in comparison to the chemical bonding potential of the previously investigated synthesized polyalkenoic acid (s-PA) co-polymer consisting of 90 w/w% acrylic and 10 w/w% maleic acid units. In addition, the analysis was carried out for enamel and dentin samples. PAA revealed a significantly lower bonding effectiveness with only half of its carboxyl groups bonded to HAp versus about two-third of the carboxyl groups of s-PA. The difference in bonding potential was confirmed by the considerably lower adhesiveness of PAA to enamel and dentin as compared to that of s-PA The present findings indicate that the molecular structure of the polyalkenoic acid significantly influences the chemical bonding efficacy to Hap-based substrates. (C) 2003 Published by Elsevier Science Ltd.

書籍

  • Dental Biomaterials
    Woodhead publishing 2008年
  • Fundamentals of Operative Dentistry. A Comtemporary Approach (3rd Ed)
    Quintessence Publishing 2006年

作品等

  • 歌登町における永久歯う蝕予防対策
    1989年 -2011年

その他活動・業績

  • Anri Fukuoka, Kenichi Koshiro, Satoshi Inoue, Yasuhiro Yoshida, Toru Tanaka, Takatsumi Ikeda, Kazuomi Suzuki, Hidehiko Sano, Bart Van Meerbeek JOURNAL OF ADHESIVE DENTISTRY 13 (3) 243 -248 2011年06月 [査読無し][通常論文]
     
    Purpose: To evaluate the hydrolytic stability of three one-step self-etching adhesives (1-SEAs) bonded to dentin through bond strength testing and ultra-morphological interfacial analysis before and after long-term thermocycling. Materials and Methods: Eighteen flattened mid-coronal dentin surfaces of extracted human molars were subjected to bonding treatment using three 1-SEAs (Clearfil S(3) Bond, Kuraray (S3), G-Bond, GC (GB), Absolute, Dentsply-Sankin (AB)), after which the bonded surfaces were built up with composite. After storage overnight at 37 C, the specimens were sectioned into slabs and further trimmed into an hourglass shape with an interface area of approximately 1 mm(2). The specimens were left untouched (control) or were thermocycled for 100,000 cycles. The microtensile bond strength (mu TBS) was measured and the ultrastructure of the adhesive/dentin interface characterized using transmission electron microscopy (TEM). Results: Long-term thermocycling significantly decreased the mu TBS of all one-step adhesives tested (p < 0.05, one-way ANOVA and Games-Howell test). TEM revealed a similar interfacial ultrastructure before and after thermocycling for S3. For GB, many voids were observed at the interface after 100,000 thermocycles. Regarding AB, collagen fibrils could no longer be clearly observed upon staining, while the adjacent unaffected dentin was rich in voids. Conclusion: The bond strength and ultramorphological data demonstrated that the bond of 1-SEAs to dentin degrades with time, although the degree of degradation is obviously material dependent.
  • 日本歯科保存学雑誌 52 (1) 27 -38 2009年 [査読無し][通常論文]
  • Takatsumi Ikeda, Jan De Munck, Kenichi Shirai, Kazuhiro Hikita, Satoshi Inoue, Hidehiko Sano, Paul Lambrechts, Bart Van Meerbeek DENTAL MATERIALS 24 (10) 1316 -1323 2008年10月 [査読無し][通常論文]
     
    Objectives. The objectives of this study were (1) to clarify the relationship between the duration of air-drying of one-step self-etch adhesives (1-SEAs) and the evaporation degree (ED) of solvents, and (2) to evaluate the effect of ED on the ultimate micro-tensile strength (mu TS) of the adhesives. Methods. The ED of one HEMA-rich I-SEA, Clearfil S' Bond (Kuraray), and two HEMA-free 1-SEAs, iBond (Heraeus-Kulzer) and G-Bond (GC), was determined without air-drying and after, respectively, 5- and 10-s air-drying using a gravimetric method. Next, the mu TS of the adhesives at ED equivalent to 0-, 5- and 10-s air-drying was measured. Results. The ED increased with extension of air-drying time. Among the adhesives tested, iBond showed the largest ED, followed by G-Bond and Clearfil S-3 Bond in this order. A longer air-drying time for 10-s resulted in a statistically significantly higher mu TS for the HEMA-rich Clearfil S3 Bond. The mu TS of the latter was higher than that of the other two HEMA-free adhesives for each air-drying time. Significance. Air-drying of 1-SEAs had a significant effect on the degree of solvent evaporation (ED) and also on the mechanical properties (mu TS) of the 1-SEAs upon setting. It is therefore beneficial to remove solvents of the 1-SEAs as much as possible by thorough, strong air-drying in order to achieve a strong adhesive layer at the interface. (C) 2008 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • 中沖 靖子, 神島 奈穂子, 池田 考績, 井上 哲, 佐野 英彦 接着歯学 26 (2) 148 -153 2008年 [査読無し][通常論文]
  • M. Peumans, J. De Munck, K. L. Van Landuyt, P. Kanumilli, Y. Yoshida, S. Inoue, P. Lambrechts, B. Van Meerbeek DENTAL MATERIALS 23 (6) 749 -754 2007年06月 [査読無し][通常論文]
     
    Objectives. Flexure at the tooth cervix has been suggested to dislodge cervical restorations more rapidly The objective of this randomized controlled clinical trial was to test the hypothesis that a higher composite flexibility better withstands tooth flexure stress. Methods. One hundred and forty-two non-carious cervical lesions were restored with composites with contrasting stiffness. Seventy-one patients randomly received two cervical restorations placed following two out of the three experimental groups: (1) the three-step etch-and-rinse adhesive Permaquick applied with the stiff micro-hybrid composite Amelogen Hybrid (PMQ/A-Hy, Ultradent), (2) Permaquick applied with the more flexible micro-filled composite Amelogen Microfill (PMQ/A-Mi, Ultradent), or (3) the 'golden standard' three-step etch-and-rinse adhesive Optibond FL applied with the micro-hybrid composite Prodigy (O-FL/Pro, Kerr). Results. The recall rate at 7 years was 80%. No statistically significant difference was found for any of the parameters evaluated neither between both adhesives, nor between both composites (McNemar, p > 0.05). Eleven percent of the O-FL/Pro restorations were clinically unacceptable due to loss of retention (5.5%) and severe marginal discoloration (5.5%). In the PMQ-group, 22% of the PMQ/A-Mi restorations (8% loss of retention, 5% severe enamel margin defects, 3% severe dentin margin defects, 6% severe marginal discoloration, 3% extreme sensitivity) and 19% of the PMQ/A-Hy restorations (13% loss of retention, 3% severe enamel margin defects, 3% severe marginal discoloration) needed repair or replacement. Significance. The clinical performance of the three adhesive/composite combinations was good and reliable during the 7-year clinical trial. It was concluded that the composite stiffness does not affect the clinical longevity of cervical composite restorations. (C) 2006 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
  • 中沖 靖子, 神島 奈穂子, 池田 考績, 井上 哲, 佐野 英彦 接着歯学 25 (3) 244 -250 2007年 [査読無し][通常論文]
  • Jan De Munck, R. Banu Ermis, Kenichi Koshiro, Satoshi Inoue, Takatsumi Ikeda, Hidehiko Sano, Kirsten L. Van Landuyt, Bart Van Meerbeek JOURNAL OF DENTISTRY 35 (1) 74 -83 2007年01月 [査読無し][通常論文]
     
    Objective: Phase-separation within HEMA-free all-in-one dental adhesives may result in the entrapment of droplets within the adhesive resin. Strongly air-blowing prior to polymerization, can remove most of these droplets. The objective of this study was to evaluate the effect these droplets may have on the resistance of the adhesive-tooth interface to NaOCl degradation. Methods: The micro-tensile bond strength (mu TBS) to enamel and dentin was determined when a HEMA-free all-in-one adhesive was applied either following a mild or strong air-blowing technique. The bonds were also exposed to an aqueous sodium hypochlorite (NaOCl) solution for 1 h, following a recently introduced methodology to mimic in vivo bond degradation. Results: This study revealed that strong air-blowing of the adhesive only resulted in a significantly higher micro-tensile bond strength (mu TBS) to dentin, but not to enamel. Likewise, NaOCl only reduced the mu TBS to dentin for both the mild and strong air-blowing technique, but again not the mu TBS to enamel. Failure analysis by SEM clearly revealed that strong air-blowing is less effective in droplet removal when the adhesive was applied in small and narrow class-I cavities, as compared to when it was applied to flat surfaces. Conclusions: NaOCl did preferentially dissolve the hybrid layer at dentin, and more for the mild than for the strong air-blowing technique. A strong air-blowing procedure resulted in a more NaOCl-resistant hybrid layer, so that it can be concluded that a HEMA-free one-step adhesive definitely benefits from a strong air-blowing technique. (C) 2006 Elsevier Ltd. All rights reserved.
  • Journal of Dental Research 86 (7) 656 -661 2007年 [査読無し][通常論文]
  • Yasuko NAKAOKI, Naoko KAMISHIMA, Takatsumi IKEDA, Satoshi INOUE, Hidehiko SANO Adhesive Dentistry 25 (3) 244 -250 2007年 [査読無し][通常論文]
  • J. De Munck, K. Shirai, Y. Yoshida, S. Inoue, K. L. Van Landuyt, P. Lambrechts, K. Suzuki, H. Shintani, B. Van Meerbeek OPERATIVE DENTISTRY 31 (4) 456 -465 2006年07月 [査読無し][通常論文]
     
    Adhesive-dentin interfaces degrade with time. This study determined the effect water storage may have on the bonding effectiveness of adhesives to occlusal Class I cavity-bottom dentin. Six adhesives, all representing contemporary classes of adhesives, were applied: a 3-step (OptiBond FL, Kerr) and 2-step (Scotchbond 1*, 3M ESPE) etch-and-rinse adhesive, a 2-step (Clearfil SE, Kuraray) and 1-step (Adper prompt, 3M ESPE) self-etch adhesive and a 2-step (FujiBond LC, GC) and 1-step (Reactmer, Shofu) resin-modified glass-ionomer adhesive. Bonding effectiveness was assessed by microtensile bond strength testing (mu TBS) and electron microscopy (Feg-SEM and TEM). The mu TBS was determined after 1 day and 1 year water storage of the entire restored cavity (indirect exposure of the adhesive-dentin interface to water) and prepared mu TBS-beams (direct exposure of the adhesive-dentin interface to water). The hypotheses tested were: (1) resin-dentin bonds formed at the bottom of Class I cavities resist 1-year water storage and (2) an adjacent composite-enamel bond protects the composite-dentin bond against degradation. Non-parametric Kruskal-Wallis analysis statistically analyzed the mu TBSs. The first hypothesis was rejected, as only the mu TBS of OptiBond FL and Clearfil SE did not significantly decrease after 1-year direct and/or indirect water storage. The second hypothesis was corroborated, as the bonding effectiveness of most simplified adhesives (Scotchbond 1, Adper Prompt, FujiBond LC and Reactmer) approached 0 (because of the frequent pre-testing failures) after 1-year direct water exposure. The second hypothesis concluded that the 3-step etch-and-rinse adhesive must still be regarded the "gold standard." Though mu TBS decreased significantly, Clearfil SE, as a 2-step self-etch adhesive, was the only simplified adhesive to perform reliably after 1-year direct water exposure.
  • K Koshiro, SK Sidhu, S Inoue, T Ikeda, H Sano JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 77B (2) 401 -408 2006年05月 [査読無し][通常論文]
     
    The purpose of this study was to evaluate the morphological appearance of resin-dentin interface produced by 1-step adhesive systems in order to classify them, using TEM. It was hypothesized that all interfaces produced by each 1-step adhesive show characteristics similar to that produced by the conventional self-etching system rather than that produced by etch-and-rinse system. The flattened dentin surfaces were subjected to bonding treatment using either a 2-step adhesive: single bond, mega bond, unifil bond; or a 1-step adhesive: Prompt L-Pop, absolute, xeno CF, AQ bond, one-up bond F, G bond, and SSB-200. A flowable resin was then applied to these surfaces. The results revealed that resin-dentin interfaces produced by 1-step systems were classified into three categories. Additionally, the interface produced by G bond and SSB-200 showed a different morphological appearance from that of the others. The interface produced by these adhesives was thinner and less demineralized, with the presence of hydroxyapatite-coated collagen fibrils. It is proposed that "Nanointeraction Zone (NIZ)" is a new complementary classification for partially demineralized resin-dentin interdiffusion zones. Thus, one-step self-etching adhesive systems can be classified into three categories, etch-and-rinse type, mild self-etching type, and NIZ type, by their morphological appearance of the interface. (c) 2006 Wiley Periodicals, Inc.
  • 池田 考績, 井上 哲, 中沖 靖子, 佐野 英彦 接着歯学 24 (2) 83 -87 2006年 [査読無し][通常論文]
  • J Dent Res 85 (4) 349 -353 2006年 [査読無し][通常論文]
  • Koshiro K., Inoue S., Sano H., De Munck J., Van Meerbeek B.: In vivo degradation of resin-dentin bonds produced by a self-etch and an etch-and-rinse adhesive. Euro J Oral Sci 113(4): 341-348, 2005.*
    2005年 [査読無し][通常論文]
  • Abe, Y., Braem, M.J.A., Lambrechts, P., Inoue, S., Takeuchi, M. and Van Meerbeek, B.: Fatigue behavior of packable composites, Biomaterials 26(17): 3405-3409, 2005.*
    2005年 [査読無し][通常論文]
  • Shirai K., De Munck J., Yoshida Y., Inoue S., Lambrecht P., Suzuki K., Shintani H., Van Meerbeek B.: Effect of cavity configuration and aging on the bonding effectiveness of six adhesives to dentin. Dent Mater 21(2): 110-124, 2005.*
    2005年 [査読無し][通常論文]
  • Ikeda T, De Munck J, Shirai K, Hikita K, Inoue S, Sano H, Lambrechts P, Van Meerbeek B: Effect of fracture strength of primer-adhesive mixture on bonding effectiveness. Dent Mater 21(5): 413-420, 2005.*
    2005年 [査読無し][通常論文]
  • Inoue S., Koshiro K., Yoshida Y., De Munck J., Nagakane K., Suzuki K., Sano H., Van Meerbeek B.: Hydrolytic stability of self-etch adhesives bonded to dentin. J Dent Res 84(12): 1160-1164, 2005.*
    2005年 [査読無し][通常論文]
  • Koshiro K., Inoue S., Niimi K., Koase K., Sano H.: Bond strength and SEM observation of CO2 laser irradiated dentin bonded with simplified-step adhesives. Oper Dent 30(2): 170-179, 2005.*
    2005年 [査読無し][通常論文]
  • Van Landuyt K., De Munck J., Snauwaert J., Coutinho E., Poitevin A., Yoshida Y., Inoue S., Peumans M., Suzuki K., Lambrechts P., Van Meerbeek B.: Monomer-solvent phase-separation in one-step self-etch adhesives. J Dent Res 84(2): 183-188, 2005.*
    2005年 [査読無し][通常論文]
  • Ikeda T., De Munck J., Shirai K., Hikita K., Inoue S., Sano H., Lambrechts P., Van Meerbeek B.: Effect of evaporation of primer components on ultimate tensile strengths of primer-adhesive mixture. Dent Mater 21(11): 1051-1058, 2005.*
    2005年 [査読無し][通常論文]
  • Nakaoki Y., Sasakawa W., Horiuchi S., Nagano F., Ikeda T., Tanaka T., Inoue S., Uno S., Sano H., Sidhu SK.: Effect of double-application of all-in-one adhesives on dentin bonding. J Dent. 33(9), 765-72, 2005.*
    2005年 [査読無し][通常論文]
  • Sasakawa W., Nakaoki Y., Nagano F., Horiuchi S., Ikeda T., Tanaka T., Noda M., Inoue S., Sano H., Sidhu S.: Micro-shear bond strength of five single-step adhesives to dentin. Dent Mater J 24(4): 617-627, 2005.*
    2005年 [査読無し][通常論文]
  • Koshiro K., Inoue S., Tanaka T., Koase K., Fujita M., Hashimoto M., Sano H.: "In vivo degradation of resin-dentin bonds produced by a self-etch vs a total-etch adhesive system", Eur J Oral Sci, 112(4): 368-375 (2004)*
    2004年 [査読無し][通常論文]
  • Koase K., Inoue S., Noda M., Tanaka T., Kawamoto C., Takahashi A., Nakaoki Y., Sano H.: "Effect of bur ground dentin on bond strength using two all-in-one and two-step adhesive systems", J Adhesive Dent, 6(2): 97-104 (2004)*
    2004年 [査読無し][通常論文]
  • 研削面の違いがレジンセメントと接着システムの歯質接着性に及ぼす影響
    日歯保存誌 47 (1) 87 -109 2004年 [査読無し][通常論文]
  • De Munck J., Braem M., Wevers W., Yoshida Y., Inoue S., Suzuki K., Lambrechts P., Van Meerbeek B.: "Micro-rotary fatigue of tooth-biomaterial interfaces", Biomaterials, 26: 1145-1153 (2004)*
    2004年 [査読無し][通常論文]
  • Inoue S., Van Meerbeek B., Abe Y., Yoshida Y., Sano H., Suzuki K., Lambrechts P.: "Effect of conditioner on bond strength of glass-ionomer adhesive to dentin/enamel with and without smear layer interposition", Oper Dent, 29(6): 685-92 (2004)*
    2004年 [査読無し][通常論文]
  • Yoshida Y., Nagakane K., Fukuda R., Nakayama Y., Okazaki M., Shintani H., Inoue S., Tagawa Y., Suzuki K., De Munck J., Van Meerbeek B.: "Comparative study on adhesive performance of functional monomers", J Dent Res, 83(6): 454-458 (2004)*
    2004年 [査読無し][通常論文]
  • Sevgican F., Inoue S., Koase K., Kawamoto C., Ikeda T., Sano H.: "Bond strength of simplified-step adhesives to enamel prepared with two different diamond burs", Aus Dent J, 49(3): 141-145 (2004)*
    2004年 [査読無し][通常論文]
  • De Munck J., Van Meerbeek B., Yoshida Y., Inoue S., Suzuki K., Lambrechts P.: "Four-year degradation of a resin-modified glass-ionomer adhesive bonded to dentin", Eur J Oral Sci, 112(1): 73-83 (2004)*
    2004年 [査読無し][通常論文]
  • De Munck, J., Van Meerbeek, B., Yoshida, Y., Inoue, S., Vargas, M., Suzuki, K., Lambrechts, P., Vanherle, G.: "Four-year water degradation of total-etch adhesives bonded to dentin", J Dent Res 82: 136-140(2003)*
    2003年 [査読無し][通常論文]
  • De Munck, J., Van Meerbeek, B., Inoue, S., Vargas, M., Yoshida, Y., Armstrong, S., Lambrechts, P., Vanherle, G.: "Micro-tensile bond strengths of one- and two-step self-etch adhesives to bur-cut enamel and dentin", Am J Dent 16(6): 414-420(2003)*
    2003年 [査読無し][通常論文]
  • Inoue, S., Vargas, M., Abe, Y., Yoshida, Y., Lambrechts, P., Vanherle, G., Sano, H. Van Meerbeek, B.: "Micro-tensile bond strength of eleven contemporary adhesives to enamel", Am J Dent 16(5): 329-334(2003)*
    2003年 [査読無し][通常論文]
  • Fukuda, R., Yoshida, Y., Nakayama, Y., Okazaki, M., Inoue, S., Sano, H., Suzuki, K., Shintani, H., Van Meerbeek, B.:" Bonding efficacy of polyalkenoic acids to hydroxyapatite, enamel and dentin", Biomaterials 24: 1861-1867(2003)*
    2003年 [査読無し][通常論文]
  • Inoue, S., Pereira, PNR., Kawamoto, C., Nakajima, M., Koshiro, K., Tagami, J., Carvalho, RM., Pashley, DH., Sano, H.:" Effect of depth and tubule direction on ultimate tensile strength of human coronal dentin", Dent Mater J 22(1): 39-47(2003)*
    2003年 [査読無し][通常論文]
  • Sano H., Inoue S., Hashimoto M., Takahashi A., Noda M., Koase K., Kawamoto C., Kubota M.: 「Long-term durability of hybridized dentin」, 13-22, ( Tagami J :『Self-etching Primer: Current status and its revolution, Proceedings of the International Symposiu・・・
    2003年 [査読無し][通常論文]
     
    Sano H., Inoue S., Hashimoto M., Takahashi A., Noda M., Koase K., Kawamoto C., Kubota M.: 「Long-term durability of hybridized dentin」, 13-22, ( Tagami J :『Self-etching Primer: Current status and its revolution, Proceedings of the International Symposium ’01 in Tokyo』, Kuraray, Tokyo)(2003)
  • Van Meerbeek B., Yoshida Y., De Munck J., Inoue S., Vargas M., Lambrechts P., Vanherle G.: 「Correlative morphologic and chemical interfacial characterization of tooth-biomaterial interactions」, 41-55 (Tagami J: 『Self-etching Primer: Current status and ・・・
    2003年 [査読無し][通常論文]
     
    Van Meerbeek B., Yoshida Y., De Munck J., Inoue S., Vargas M., Lambrechts P., Vanherle G.: 「Correlative morphologic and chemical interfacial characterization of tooth-biomaterial interactions」, 41-55 (Tagami J: 『Self-etching Primer: Current status and its revolution, Proceedings of the International Symposium ’01 in Tokyo』, Kuraray, Tokyo)(2003)
  • Inoue S., Murata Y., Sano H., Kashiwada T.: ''Effect of NaOCl treatment on bond strength between indirect resin core-buildup and dentin'',Dent Mater J, 21:343-354(2002)*
    2002年 [査読無し][通常論文]
  • Yoshida Y., Shirai K., Nakayama Y., Okazaki M., Shintani H., Inoue S., Lambrechts P., Vanherle G., Van Meerbeek B.: ''Improved filler-matrix coupling in resin composites'', J Dent Res, 81:270-273(2002)*
    2002年 [査読無し][通常論文]
  • Takahashi A., Inoue S., Kawamoto C., Ohminato R., Tanaka T., Sato Y., Pereira P.N.R., Sano H.: ''In vivo long-term durability of bond to dentin by two adhesive systems'', J Adhesive Dent, 4:151-159(2002)*
    2002年 [査読無し][通常論文]
  • Yoshioka M , Yoshida Y., Inoue S., Lambrechts P., Vanherle G., Nomura Y., Okazaki M., Shintani H., Van Meerbeek B.: ''Adhesion/decalcification mechanisms of acid interactions with human hard tissues'', J Biomed Mater Res, 59:56-62(2002)*
    2002年 [査読無し][通常論文]
  • 接着歯学<日本接着歯学会編>
    医歯薬出版 2002年 [査読無し][通常論文]
  • Abe Y., Lambrechts P., Inoue S., Van Meerbeek B., Braem MJA, Takeuchi M., Vanherle G.: Dynamic elastic modulus of ‘packable’ composites. Dent Mater 17: 520-525, 2001.*
    2001年 [査読無し][通常論文]
  • Van Meerbeek B., Vargas M., Inoue S., Yoshida Y., Peumans M., Lambrechts P., Vanherle G.: Adhesives and cements to promote preservation dentistry. Oper Dent (Supplement) 6: 119-144, 2001.*
    2001年 [査読無し][通常論文]
  • Inoue H., Inoue S., Uno S., Takahashi A., Sano H.: Micro-tensile bond strength of two single step adhesive systems to bur-prepared dentin. J Adhesive Dent 3: 129-136, 2001.*
    2001年 [査読無し][通常論文]
  • Uno S., Inoue H., Finger W.J., Inoue S., Sano H.: Micro-tensile bond strength evaluation of three adhesive systems in cervical dentin cavities. J Adhesive Dent 3: 333-341, 2001.*
    2001年 [査読無し][通常論文]
  • Inoue S., Vargas M., Abe Y., Yoshida Y., Lambrechts P., Vanherle G., Sano H.: Van Meerbeek B.: Micro-tensile bond strength of eleven contemporary adhesives to dentin. J Adhesive Dent 3: 237-245, 2001.*
    2001年 [査読無し][通常論文]
  • Inoue S., Van Meerbeek B., Abe Y., Yoshida Y., Lambrechts P., Vanherle G., Sano H.: Effect of remaining dentin thickness and the use of conditioner on micro-tensile bond strength of glass-ionomer adhesive. Dent Mater 17: 445-455, 2001.*
    2001年 [査読無し][通常論文]
  • Inoue H., Inoue S., Uno S., Komatsu H., Sano H.: Micro-tensile bond strength of two self-etching priming adhesive systems to occlusal and buccal dentin. 115-122, In ‘’Caries Preventive Restoration’’ Forum in Sapporo ‘99, Komatsu H., Inoue S., Sano H (e・・・
    2001年 [査読無し][通常論文]
     
    Inoue H., Inoue S., Uno S., Komatsu H., Sano H.: Micro-tensile bond strength of two self-etching priming adhesive systems to occlusal and buccal dentin. 115-122, In ‘’Caries Preventive Restoration’’ Forum in Sapporo ‘99, Komatsu H., Inoue S., Sano H (eds), Tokyo, GC, 2001.
  • 井上 哲, 阿部 泰彦, 吉田 靖弘, 佐野 英彦 『接着歯学』 18 (2) 93 -99 2000年 [査読無し][通常論文]
  • 井上 哲, 阿部 泰彦, 佐野 英彦 『接着歯学』 18 (2) 85 -92 2000年 [査読無し][通常論文]
  • Kaneko J., Inoue S., Kawakami S. and Sano H.: ''Bleaching effect of sodium percarbonate on discolored pulpless teeth in vivo'', J Endodon 26: 25-28 (2000)*
    2000年 [査読無し][通常論文]
  • Van Meerbeek B., Vargas M., Inoue S., Yoshida Y., Perdigão J., Lambrechts P., Vanherle G.: ''Microscopy Investigations – Techniques, Results, Limitations'', Am J Dent, 13: 3D-18D (2000)*
    2000年 [査読無し][通常論文]
  • Noda M., Komatsu H., Inoue S. and Sano H.: ''Antibiotic susceptibility of bacteria detected from root canal exudate of persistent apical periodontitis'', J Endodon 26: 221-224 (2000)*
    2000年 [査読無し][通常論文]
  • 「新規1ステップ接着システムの象牙質研削面に対する接着」
    『日歯保存誌』 43 1211 -1219 2000年 [査読無し][通常論文]
  • Inoue S., Van Meerbeek B., Vargas M., Yoshida Y., Lambrechts P. and Vanherle G.: ''Adhesion mechanism of self-etching adhesives.'', 131-148, (Tagami J, Toledano M, Prati: Advanced Adhesive Dentistry 3rd International Kuraray Symposium, Como: Grafiche E・・・
    2000年 [査読無し][通常論文]
     
    Inoue S., Van Meerbeek B., Vargas M., Yoshida Y., Lambrechts P. and Vanherle G.: ''Adhesion mechanism of self-etching adhesives.'', 131-148, (Tagami J, Toledano M, Prati: Advanced Adhesive Dentistry 3rd International Kuraray Symposium, Como: Grafiche Erredue, Italy)(2000)
  • Van Meerbeek B., Vargas M., Inoue S., Yoshida Y., Perdigao J., Lambrechts P., Vanherle G.: ''Evolution des adhesives dentinaires: evaluation microscopique'', (Dejou J., Camps J.: Proceedings of “17emes Journees du College Francais de Biomateriaux Denta・・・
    2000年 [査読無し][通常論文]
     
    Van Meerbeek B., Vargas M., Inoue S., Yoshida Y., Perdigao J., Lambrechts P., Vanherle G.: ''Evolution des adhesives dentinaires: evaluation microscopique'', (Dejou J., Camps J.: Proceedings of “17emes Journees du College Francais de Biomateriaux Dentaires. BIOMAT 2000: Bio-Adhesion et esthetique, du laboratoire a la clinique.)(2000)
  • Van Meerbeek B., Inoue S., Perdigao J., Lambrechts P. and Vanherle G.: ''Enamel and dentin adhesion'', 178-235 (Schwartz RS, Summitt JB, Robbins JW: Fundamentals of Operative Dentistry. A Comtemporary Approach, Quintessence Publishing, Carol Stream, IL・・・
    2000年 [査読無し][通常論文]
     
    Van Meerbeek B., Inoue S., Perdigao J., Lambrechts P. and Vanherle G.: ''Enamel and dentin adhesion'', 178-235 (Schwartz RS, Summitt JB, Robbins JW: Fundamentals of Operative Dentistry. A Comtemporary Approach, Quintessence Publishing, Carol Stream, IL, USA)(2000)
  • Uno S, Tanaka T, Inoue S, Sano H.:''The influences of configuration factors on the cavity adaptation in compomer restorations'', Dental Materials Journal, 18: 19-31 (1999)*
    1999年 [査読無し][通常論文]
  • Uno S, Tanaka T, Inoue S, Sano H.: ''The influences of configuration factors on the cavity adaptation in compomer restorations'', Dental Materials Journal 18: 19-31.
    1999年 [査読無し][通常論文]
  • Noda M, Inoue S, Komatsu H.:''A comparison of methods for detecting bacteria in root canal exudate'', J Endodon, 25, 187-189 (1999)*
    1999年 [査読無し][通常論文]
  • Noda M, Inoue S, Komatsu H.: ''A comparison of methods for detecting bacteria in root canal exudate'', J Endodon 25: 187-189.
    1999年 [査読無し][通常論文]
  • Sano H, Inoue S, Noda M, Kawamoto C, Uno S, Takahashi A, Aoyagi M, Sato Y. ''Long-term durability of resin-dentin bonds'', 31-38, Modern trends in adhesive dentistry, Proceedings of the Adhesive Dentistry Forum '99 in Tsurumi, Osaka, Kuraray.
    1999年 [査読無し][通常論文]
  • Sano H., Inoue S., Noda M., Kawamoto C., Uno S., Takahashi A., Aoyagi M. and Sato Y.: ''Long-term durability of resin-dentin bonds'', 31-38 (Momoi Y., Akimoto N., Kohno A., Modern trends in adhesive dentistry, Proceedings of the Adhesive Dentistry Foru・・・
    1999年 [査読無し][通常論文]
     
    Sano H., Inoue S., Noda M., Kawamoto C., Uno S., Takahashi A., Aoyagi M. and Sato Y.: ''Long-term durability of resin-dentin bonds'', 31-38 (Momoi Y., Akimoto N., Kohno A., Modern trends in adhesive dentistry, Proceedings of the Adhesive Dentistry Forum ’99 in Tsurumi, Osaka, Kuraray) (1999)
  • Sano H., Uno S. and Inoue S.: ''Clinical consideration of dentin adhesion'', 1-13 (Hidehiko Sano, Shigeru Uno and Satoshi Inoue, Modern trends in Adhesive Dentistry: Proceedings of the Adhesive Forum in Sapporo 98, Kuraray, Osaka) (1998)
    1998年 [査読無し][通常論文]
  • Uno S., Tanaka T., Inoue S. and Sano H.:''A consideration of compomer restorations: variables affecting cavity adaptation'',87-99 (Hidehiko Sano, Shigeru Uno and Satoshi Inoue, Modern trends in Adhesive Dentistry: Proceedings of the Adhesive Forum in S・・・
    1998年 [査読無し][通常論文]
     
    Uno S., Tanaka T., Inoue S. and Sano H.:''A consideration of compomer restorations: variables affecting cavity adaptation'',87-99 (Hidehiko Sano, Shigeru Uno and Satoshi Inoue, Modern trends in Adhesive Dentistry: Proceedings of the Adhesive Forum in Sapporo 98, Kuraray, Osaka) (1998)
  • Sano H, Uno S, Inoue S.:''Modern trends in adhesive dentistry. Proceedings of the Adhesive Dentistry Forum '98 in Sapporo'', 126 ( Kuraray, Osaka) (1998)
    1998年 [査読無し][通常論文]

受賞

  • 2000年07月 Academy of Dental Materials Award of the Academy of Dental Materials
     
    受賞者: 井上 哲

共同研究・競争的資金等の研究課題

  • 文部科学省:科学研究費補助金(基盤研究(C))
    研究期間 : 2010年 -2013年 
    代表者 : 井上 哲
     
    H24年度は、各種1ステップ接着システムの微小引張り接着強さを再測定し、またその前の23年度から引き続き行っていた臨床的スミア層溶解能の透過型電子顕微鏡(TEM)による観察を行った。さらに、予定より少し遅れたものの、100,000回のサーマルサイクリング(TC)負荷試験を開始しH25年6月に終了予定であるので、H25年度に負荷後試料の微小引張り接着試験とTEM観察を行う予定である。1.接着強さの再測定TC試験は当初岡山大学で行う予定であったが、本学の設備で行うこととしたため、試料の形態を変える必要性が生じた。そのため、その形態に合わせたコントロールの接着強さを再測定した。ヒト抜去健全大臼歯歯冠部を水平に切断後、2種の臨床的スミア層を産生するためダイヤモンドポイント(レギュラー)および耐水研磨紙(#2000)を用いて可及的平坦な被着象牙質面を得た。この面に対し、Clearfil Tri-S Bond(S3), Hybrid Coat II(HC), BeautiBond(BB), Bond Force(BF), Absolute2(AB),あるいはG-Bond Plusの接着システムを用いて処理し、通法に従って微小引張り試験を行ったが、試料の形態はダンベル型ではなくスティック(ビーム)型とした。その結果、前年度とほぼ同様の値が得られた。2.TEM観察スミア層の溶解能を検討するためにH22年度に表面をSEM観察した試料を用い、H23年度から継続して行っていたTEM観察の結果、ABではレギュラー、#2000によらず、スミア層は脱灰され、コラーゲン線維網と思われる像が観察されたが、そのほかのシステムではレギュラー研削面のほうがスミア層が残存している像が観察された。
  • 文部科学省:科学研究費補助金(基盤研究(C))
    研究期間 : 2006年 -2008年 
    代表者 : 井上 哲, 田中 享, 池田 考績, 福岡 杏理
     
    簡便で短時間に処置が終了し、化学的に歯質と接着し、さらに長期にわたり安定した接着性能を発揮できる接着システムの開発を最終目的とし、その基礎的知見を得るために、臨床的な条件下で作製したスミア層を形態学的に解析するとともに、各種スミア層を有する歯面に対する簡略化ステップ接着システムの短長期的接着性能を検討した。その結果、スミア層の厚さは切削器具に左右されること、スミア層の除去能が低い接着システムでは接着性能に影響があること、1ステップ接着システムは長期的に接着性能が低下することが判明した。
  • 文部科学省:科学研究費補助金(基盤研究(C))
    研究期間 : 2003年 -2005年 
    代表者 : 井上 哲, 吉田 靖弘, 池田 孝績, 田中 享
     
    1.短期的接着性能に関する研究(1)研削面の違いが接着性能へ及ぼす影響2種のダイヤモンドポイントを用いて形成した歯質研削面に対する各種接着システムの短期的接着性能を、微小引張り接着強さとその破断面のSEM観察の結果、1ステップ型接着システムでは、象牙質接着に対して有利な研削面を形成するためのバー選択が必要であると考えられた。(2)簡略化ステップ型接着システムの接着界面分類1ステップ型接着システムを、#600SiC研磨象牙質面へ接着した場合、その接着界面は(1)樹脂含浸層形成型、(2)機能性傾斜材料型、(3)ナノ相互作用層形成型に分類された。特に(3)は脱灰される歯質の量が少なく、レジンの浸透が充分に期待できるため、劣化の少ない長期に安定な材料であることが期待された。2.長期的接着性能に関する研究(1)2ステップ型セルフエッチングシステム3種の2ステップ型セルフエッチングシステムの加水分解に対する安定性を、100000回サーマルサイクリング試験後の接着強さならびにTEMによる接着界面像を指標に検討した。その結果、接着界面の長期安定性は、機能性モノマー自体の加水分解安定性や機能性モノマーと象牙質との化学的相互作用に依存していることが示唆された。(2)1ステップ型接着システム3種の1ステップ型接着システムについて100000回サーマルサイクリング試験前後の接着強さ測定と接着界面をTEM観察した結果、材料によりサーマルサイクリングの影響の程度は異なるものの、何らかの変化(劣化)が象牙質接着界面に起こっていることが示唆された。3.各種機能性モノマーの歯質に対する化学的相互作用の分析X線光電子分光法分析により、機能性モノマーは歯質と化学的に相互作用することが示された。10-MDPはハイドロキシアパタイトと最も強く反応するだけではなく、Caとの結合は加水分解に対し最も安定であることが判明した。
  • 文部科学省:科学研究費補助金(一般研究(C), 基盤研究(C))
    研究期間 : 1995年 -1997年 
    代表者 : 井上 哲, 小林 洋一, 田中 享
     
    難治性感染根管の原因を明らかにするためには根管内滲出物の採取分析が重要であるが、採取できる試料が微量であるために細菌叢や病態の把握が困難である。そこで試料中の嫌気性菌を簡便・確実に分離同定する方法として、採取した滲出物を増菌用培地に接種して24時間嫌気的に培養した後に検査所に試料を送る方法(増菌法)で培養同定を行い良好な結果を得た。難治性感染根管22例について調べた所、18例で何らかの菌が検出された。その内訳はα‐Streptococcus、En‐terococcusなど通性嫌気性菌8種、偏性嫌気性菌4種、好気性菌2種が検出された。Ent‐erococcusは従来の報告よりも多数検出された。以上の結果より、増菌法は従来の方法より確実に滲出物中の細菌を検出できると思われた。次に、Enterococcusは長期症例や難治性感染根管から検出される頻度が高いとの報告が近年なされていることから、同菌種の宿主細胞への影響をより詳細に検討した。分離回収可能であった5株のEnterococcusについて分子生物学的手法により比較したところ、全ての株の由来が異なっていた。また、培養細胞系に感染させ、細胞内情報伝達計(チロシンリン酸化)に及ぼす影響を調べたが、同菌の直接接触ではチロシンリン酸化に変化は認められなかった。このことから、Enterococcusは細胞間情報伝達物質を介して宿主との反応に関与しているのではないかと考えられた。そこで、さらに免疫学的手法を用いて原因を明らかにする必要があると思われた。
  • 歯質接着システムに関する研究
    研究期間 : 1994年
  • Adhesion between tooth substrates and dental adhesives
    研究期間 : 1994年

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