研究者データベース

青沼 仁志(アオヌマ ヒトシ)
電子科学研究所 附属社会創造数学研究センター
准教授

基本情報

所属

  • 電子科学研究所 附属社会創造数学研究センター

職名

  • 准教授

学位

  • 博士(理学)(1998年03月 北海道大学)

ホームページURL

科研費研究者番号

  • 20333643

ORCID ID

Researcher ID

  • D-6540-2012

J-Global ID

プロフィール

  • 動物がどのように環境を知覚し,適応的な行動を実時間で発現するのか?その基盤となる脳神経系のメカニズムと,脳・身体・環境の相互作用によって生まれる機能の発生メカニズムを研究しています.

研究キーワード

  • 情報機械   超高速度運動   コオロギ   機械感覚細胞   cGMP   感覚情報   一酸化窒素   エネルギー増幅機構   機械感覚器   動的モデリング技術   光学計測   脳・神経   感覚器   cAMP   多細胞化   行動学   進化   神経生理学   神経行動学   神経修飾物   適応行動   自律分散システム   昆虫   社会行動   Neuromodulation   adaptive behavior   Decentralized autonomous system   System model   Insect   Social behavior   

研究分野

  • ライフサイエンス / 動物生理化学、生理学、行動学
  • 情報通信 / 知能ロボティクス
  • 情報通信 / 知覚情報処理
  • ライフサイエンス / 神経科学一般
  • 情報通信 / 生命、健康、医療情報学

職歴

  • 2015年04月 - 現在 北海道大学 電子科学研究所・附属社会創造数学研究センター 准教授
  • 2006年04月 - 現在 北海道大学大学院 生命科学院 准教授
  • 2003年 - 現在 北海道大学 電子科学研究所 准教授
  • 2014年10月 - 2020年03月 科学技術振興機構 戦略的創造研究推進事業・小林CREST グループリーダー
  • 2003年07月 - 2006年03月 北海道大学大学院 工学研究科 助教授
  • 2001年01月 - 2003年07月 北海道大学 電子科学研究所 助手
  • 1999年01月 - 2000年12月 サウザンプトン大学 生物科学専攻 日本学術振興会 海外特別研究員
  • 1998年04月 - 1998年12月 サウザンプトン大学 生物科学専攻 博士研究員
  • 1995年04月 - 1997年03月 日本学術振興会 特別研究員(DC2)

学歴

  • 1993年04月 - 1998年03月   北海道大学大学院   理学研究科   生物科学専攻
  • 1991年04月 - 1993年03月   北海道大学大学院   理学研究科   動物学専攻
  • 1987年04月 - 1991年03月   北海道大学   理学部   生物学科

所属学協会

  • 日本進化学会   国際無脊椎動物神経生物学会   国際神経行動学会   日本動物学会北海道支部   日本動物学会   日本比較生理生化学会   International Society for Invertebrate Neurobiology   International Society of Neuroethology   

研究活動情報

論文

  • Hitoshi Aonuma
    The Journal of Experimental Biology 2020年09月06日 [査読有り][通常論文]
  • Hitoshi Aonuma
    Journal of Robotics and Mechatronics 32 4 812 - 821 2020年08月20日 [査読有り][通常論文]
     
    In general, legged robots are designed to walk with a fixed rhythmic pattern. However, most animals can adapt their limb movements while walking. It is necessary to understand the mechanism of adaptability during locomotion when designing bio-inspired legged robots. In this paper, we propose an approach to analyze the flexible locomotion pattern of animals using a polar histogram. Field crickets were used to investigate variations in leg movement of insects depending on the environment. Crickets have a tripod gait; however, their leg movement changes depending on the texture of the ground. There was a significant difference between the leg movement when walking and when swimming. Our approach can explain how animals move their legs during locomotion. This study is useful for evaluating the movements of legged robots.
  • Kazuya Saito, Ricardo Pérez-De La Fuente, Kôichi Arimoto, Seong Young ah, Hitoshi Aonuma, Ryuma Niiyama, Zhong You
    Proceedings of the National Academy of Sciences of the United States of America 117 30 17622 - 17626 2020年07月28日 [査読有り][通常論文]
     
    © 2020 National Academy of Sciences. All rights reserved. Technologies to fold structures into compact shapes are required in multiple engineering applications. Earwigs (Dermaptera) fold their fanlike hind wings in a unique, highly sophisticated manner, granting them the most compact wing storage among all insects. The structural and material composition, in-flight reinforcement mechanisms, and bistable property of earwig wings have been previously studied. However, the geometrical rules required to reproduce their complex crease patterns have remained uncertain. Here we show the method to design an earwig-inspired fan by considering the flat foldability in the origami model, as informed by X-ray microcomputed tomography imaging. As our dedicated designing software shows, the earwig fan can be customized into artificial deployable structures of different sizes and configurations for use in architecture, aerospace, mechanical engineering, and daily use items. Moreover, the proposed method is able to reconstruct the wing-folding mechanism of an ancient earwig relative, the 280-million-year-old Protelytron permianum. This allows us to propose evolutionary patterns that explain how extant earwigs acquired their wing-folding mechanism and to project hypothetical, extinct transitional forms. Our findings can be used as the basic design guidelines in biomimetic research for harnessing the excellent engineering properties of earwig wings, and demonstrate how a geometrical designing method can reveal morphofunctional evolutionary constraints and predict plausible biological disparity in deep time.
  • Hitoshi Aonuma
    Frontiers in Physiology 2020年03月17日 [査読有り][通常論文]
  • Hitoshi Aonuma
    HardwareX 2020年03月 [査読有り][通常論文]
  • Daiki Wakita, Katsushi Kagaya, Hitoshi Aonuma
    Journal of The Royal Society Interface 17 162 20190374 - 20190374 2020年01月 [査読有り][通常論文]
  • Hitoshi Aonuma
    Scientific Reports 2019年12月02日 [査読有り][通常論文]
  • Daiki Wakita, Yumino Hayase, Hitoshi Aonuma
    Scientific Reports 9 1 8298  2019年12月 [査読有り][通常論文]
  • Hitoshi Aonuma
    Frontiers in Neurorobotics 2019年08月23日 [査読有り][通常論文]
  • Daiki Wakita, Hitoshi Aonuma, Shin Tochinai
    Cold Spring Harbor Laboratory 2019年06月 [査読有り][通常論文]
  • Hitoshi Aonuma
    Frontiers in behavioral neuroscience 13 65  2019年04月02日 [査読有り][通常論文]
  • Elizabeth G. Clark, Daichi Kanauchi, Takeshi Kano, Hitoshi Aonuma, Derek E. G. Briggs, Akio Ishiguro
    The Journal of Experimental Biology 222 Pt 2 jeb.192104  2019年01月15日 [査読有り][通常論文]
  • Hitoshi Aonuma
    Journal of insect physiology 112 117 - 122 2019年01月 [査読有り][通常論文]
  • Takayuki Watanabe, Atsushi Ugajin, Hitoshi Aonuma
    eneuro 5 4 ENEURO.0061 - 18.2018 2018年07月07日 [査読有り][通常論文]
  • 園山貴之, 荻本啓介, 石橋敏章, 須田有輔, 青沼仁志, 青沼仁志, 松浦啓一
    魚類学雑誌 65 1 81‐84 - 84 日本魚類学会 2018年04月25日 [査読無し][通常論文]
     
    <p>A specimen of the pufferfish <i>Torquigener albomaculosus</i>, previously recorded in depths of 10–30 m off Amami-oshima Island, northern Ryukyu Islands, was collected at 100m depth by hook and line off Hamahika-jima Island (off east coast of Okinawa-jima Island) and maintained for 136 days in an aquarium whilst swimming and eye closing behavior were observed. Unlike some other pufferfishes, which close their eyes by twisting the encircling skin, <i>T. albomaculosus</i> closes employing a vertical "blink", similar to tetrapods.</p>
  • Hitoshi Aonuma, Yuki Totani, Manabu Sakakibara, Ken Lukowiak, Etsuro Ito
    Biophysics and physicobiology 15 129 - 135 2018年 [査読有り][通常論文]
     
    To find a causal mechanism of learning and memory is a heuristically important topic in neuroscience. In the pond snail Lymnaea stagnalis, the following experimental facts have accrued regarding a classical conditioning procedure known as conditioned taste aversion (CTA): (1) one-day food-deprived Dutch snails have superior CTA memory formation; (2) the one-day food-deprived snails have a low monoamine content (e.g., serotonin, dopamine, octopamine) in their central nervous system (CNS); (3) fed or five-day food-deprived snails have poorer CTA memory and a higher monoamine content; (4) the Dutch snails form better CTA memory than the Canadian TC1 strain; and, (5) the F1 cross snails between the Dutch and Canadian TC1 strains also form poor CTA memory. Here, in one-day food-deprived snails, we measured the monoamine content in the CNSs of the 3 populations. In most instances, the monoamine content of the Dutch strain was lower than in the other two populations. The F1 cross snails had the highest monoamine content. A lower monoamine content is correlated with the better CTA memory formation.
  • Aonuma, H., Totani, Y., Kaneda, M., Nakamura, R., Watanabe, T., Hatakeyama, D., Dyakonova, V.E., Lukowiak, K., Ito, E.
    Neurobiology of Learning and Memory 148 20 - 29 2018年 [査読有り][通常論文]
  • Momohara, Y., Aonuma, H., Nagayama, T.
    Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology 204 5 465 - 473 2018年 [査読有り][通常論文]
  • DiRienzo, N., Aonuma, H.
    Animal Behaviour 138 9 - 17 2018年 [査読有り][通常論文]
  • PROCEEDINGS 2018 12TH FRANCE-JAPAN AND 10TH EUROPE-ASIA CONGRESS ON MECHATRONICS 89 - 93 2018年 [査読有り][通常論文]
  • Decentralized Control Mechanism Underlying Interlimb Coordination of Centipedes
    Kotaro Yasui, Kazuki Kikuchi, Takeshi Kano, Yumino Hayase, Shigeru Kuroda, Hitoshi Aonuma, Ryo Kobayashi, Akio Ishiguro
    Proceedings of The 8th International Symposium on Adaptive Motion of Animals and Machines(AMAM2017) 82 - 83 2017年06月 [査読有り][通常論文]
  • Hitoshi Aonuma, Mugiho Kaneda, Dai Hatakeyama, Takayuki Watanabe, Ken Lukowiak, Etsuro Ito
    NEUROBIOLOGY OF LEARNING AND MEMORY 141 189 - 198 2017年05月 [査読有り][通常論文]
     
    The pond snail Lymnaea stagnalis is capable of learning taste aversion by pairing presentations of a sucrose solution and an electric shock and consolidating it into long-term memory (LTM), which is referred to as conditioned taste aversion (CTA). We asked here if the neurotransmitter octopamine is involved in CTA. We first determined the levels of octopamine and its catabolites in the central nervous system (CNS) of snails with varying degrees of food deprivation, because CIA grades are correlated with degrees of food deprivation. We next manipulated the octopamine signaling using both an agonist and an antagonist of octopamine receptors and correlated their respective effects with CTA grades. We found that snails with the least amount of food-deprivation obtained the best CTA grade and had low levels of octopamine; whereas the most severely food-deprived snails did not form CTA and had the highest CNS octopamine levels. In modestly food-deprived snails, octopamine application increased the basal level of feeding response to a sucrose solution, and it did not obstruct CTA formation. Application of phentolamine, an octopamine receptor antagonist, to the most severely food-deprived snails decreased the basal level of feeding elicited by sucrose, but it did not enhance CTA formation. We conclude that octopamine involvement in OA formation in Lymnaea is at best weak, and that the changes in CNS octopamine content are an epiphenomenon. (C) 2017 Elsevier Inc. All rights reserved.
  • Hiroyuki Shimoji, Hitoshi Aonuma, Toru Miura, Kazuki Tsuji, Ken Sasaki, Yasukazu Okada
    BEHAVIORAL ECOLOGY AND SOCIOBIOLOGY 71 2 2017年02月 [査読有り][通常論文]
     
    How individual organisms whose behavior is potentially driven by selfish interests cooperate to form a society is a central question in evolutionary biology. Worker reproduction and its suppression in eusocial insects provide an illuminating model of such a conflict resolution. Although many theoretical and empirical studies focus on the nature and evolutionary consequences of this reproductive conflict, little is known about its physiological underpinnings. Here, we hypothesized that the dopaminergic system, which has a gonadotropic function in eusocial Hymenoptera, is controlled by social suppression via the queen presence signal and the worker-worker dominance interactions. In Diacamma sp. from Japan, the queen presence signal is transferred to workers by direct contact, and worker-worker dominance interaction occurs commonly in large colonies, even when a queen is present. Using the ant Diacamma sp., we showed that the aggressive interactions among workers suppressed brain dopamine levels of the workers. Moreover, our data suggest that the queen presence signal transmitted by direct contact suppresses the brain dopamine level and the transcription of dopamine synthetic enzyme (ddc) of workers. Our data provide clear empirical evidence that worker brain dopamine is suppressed by both social stimuli directed from dominant workers and the queen.Significance statementIn eusocial Hymenoptera, worker reproduction is suppressed by social interactions such as queen presence information and dominance interaction. Dopamine, one of the biogenic amines, is a well-known gonadotropic neurohormone in eusocial Hymenoptera. Honeybee studies revealed that the queen presence information regulated dopamine levels in worker brains. In an ant, the dominance interaction also controlled dopamine levels of workers. In a queenless ant Diacamma sp., queen presence information and dominance interaction are known to dually suppress worker reproduction. Given the above examples, dopaminergic signaling is predicted to be a general mechanism that can suppress worker reproduction in multiple ways. We here test whether both the queen-and worker-originated signals (i.e., queen presence information and dominance interaction) affect the worker dopamine level. We show that dopaminergic signaling is affected by dual social factors, suggesting the general role of dopaminergic signaling in mediating social interaction and reproductive suppression.
  • Matsuzaka, Y., Sato, E., Kano, T., Aonuma, H., Ishiguro, A.
    Biology Open 6 4 425 - 438 2017年 [査読有り][通常論文]
  • DiRienzo, N., Aonuma, H.
    Behavioral Ecology and Sociobiology 71 8 2017年 [査読有り][通常論文]
  • Shiratori, C., Suzuki, N., Momohara, Y., Shiraishi, K., Aonuma, H., Nagayama, T.
    European Journal of Neuroscience 46 3 1863 - 1874 2017年 [査読有り][通常論文]
  • Matsuda, K., Gotoh, H., Tajika, Y., Sushida, T., Aonuma, H., Niimi, T., Akiyama, M., Inoue, Y., Kondo, S.
    Scientific Reports 7 1 13939  2017年 [査読有り][通常論文]
  • Kano, T., Sato, E., Ono, T., Aonuma, H., Matsuzaka, Y., Ishiguro, A.
    Royal Society Open Science 4 12 171200  2017年 [査読有り][通常論文]
  • Ryota Matsuo, Marin Tanaka, Rena Fukata, Suguru Kobayashi, Hitoshi Aonuma, Yuko Matsuo
    JOURNAL OF COMPARATIVE NEUROLOGY 524 18 3849 - 3864 2016年12月 [査読有り][通常論文]
     
    The terrestrial slug Limax can learn to avoid the odor of some food (e.g., carrot juice) by the simultaneous presentation of an aversive stimulus (e.g., bitterness of quinidine). This type of associative memory critically depends on the higher olfactory center, the procerebrum in the central nervous system. The modulation of the local field potential (LFP) oscillation recorded on the procerebrum has been thought to reflect the information processing of the odor that elicits the behavioral change, such as avoidance of the aversively learned odor or approaching an attractive food's odor. Here we focused on octopamine, an important neuromodulator involved in learning and memory in invertebrates, and considered to be the invertebrate equivalent of noradrenaline. We identified a few octopaminergic neurons in the subesophageal and buccal ganglia, and a larger number near the procerebrum in the cerebral ganglia, using immunohistochmical staining and in situ hybridization of tyramine -hydroxylase, an octopamine-synthesizing enzyme. Application of octopamine reduced the frequency of LFP oscillation in a dose-dependent manner, and this effect was inhibited by preincubation with phentolamine. High-performance liquid chromatography analysis revealed the presence of octopamine, noradrenaline, and adrenaline in the central nervous system. Unexpectedly, noradrenaline and adrenaline both accelerated the LFP oscillation, in contrast to octopamine. Our results suggest that octopamine and noradrenaline have distinct functions in olfactory information processing, in spite of their structural similarity. J. Comp. Neurol. 524:3849-3864, 2016. (c) 2016 Wiley Periodicals, Inc.
  • Hitoshi Aonuma, Mugiho Kaneda, Dai Hatakeyama, Takayuki Watanabe, Ken Lukowiak, Etsuro Ito
    BIOLOGY OPEN 5 12 1869 - 1873 2016年12月 [査読有り][通常論文]
     
    The pond snail Lymnaea learns conditioned taste aversion (CTA) and remembers not to respond to food substances that initially cause a feeding response. The possible relationship between how well snails learn to follow taste-aversion training and brain dopamine contents is not known. We examined this relationship and found the following: first, snails in the act of eating just before the commencement of CTA training were poor learners and had the highest dopamine contents in the brain; second, snails which had an ad libitum access to food, but were not eating just before training, were average learners and had lower dopamine contents; third, snails food-deprived for one day before training were the best learners and had significantly lower contents of dopamine compared to the previous two cohorts. There was a negative correlation between the CTA grades and the brain dopamine contents in these three cohorts. Fourth, snails food-deprived for five days before training were poor learners and had higher dopamine contents. Thus, severe hunger increased the dopamine content in the brain. Because dopamine functions as a reward transmitter, CTA in the severely deprived snails (i.e. the fourth cohort) was thought to be mitigated by a high dopamine content.
  • K. Ohkawara, H. Aonuma
    INSECTES SOCIAUX 63 2 257 - 264 2016年05月 [査読有り][通常論文]
     
    Insect behavior is regulated by physiological and neurological systems. Biogenic amines play a role in motivating the behavior as neurotransmitters, neuromodulators, and neurohormones in the nervous systems. In ants, the change of brain amine levels is linked to various behavioral properties: reproduction, cooperation, and hostility. Aggressiveness is also manipulated by the neurological systems. The social structures in some ant species are established by aggressive interactions among queens. However, there are a few cases showing the putative role of biogenic amines for the queen aggression. Vollenhovia nipponica is a social parasite ant, which establishes functional monogyny that antagonistic interactions among multiple queens lead to dominance hierarchy. In this time, we examined changes in the levels of biogenic amines in heads of queens in V. nipponica, associated with the observation of aggressive behavior. In the colonies, dealate queens violently attacked toward each other by biting and pulling on body parts. Whereas, the aggressive behavior was never observed in alate queens. In the measurements in four biogenic amines, serotonin (5-HT), dopamine (DA), octopamine (OA), and tyramine (TA), the levels of 5-HT and DA in dealates were significantly higher than those in alates. The levels of OA and TA were not significantly different between them. Moreover, the similar changes were confirmed in the groups of young (10-30 days old) and old queens (more than 6-month old). In V. nipponica queens, 5-HT and DA could regulate motivation of aggression, irrespective of age. However, the changes of biogenic amines are possibly affected by other behavioral factors.
  • Yuki Ishikawa, Hitoshi Aonuma, Ken Sasaki, Toru Miura
    PLOS ONE 11 5 e0154230  2016年05月 [査読有り][通常論文]
     
    In termites, i.e. a major group of eusocial insects, the soldier caste exhibits specific morphological characteristics and extremely high aggression against predators. Although the genomic background is identical to the other non-aggressive castes, they acquire the soldier-specific behavioral character during the course of caste differentiation. The high aggressiveness and defensive behavior is essential for colony survival, but the neurophysiological bases are completely unknown. In the present study, using the damp-wood termite Hodotermopsis sjostedti, we focused on two biogenic amines, octopamine (OA) and tyramine (TA), as candidate neuromodulators for the defensive behavior in soldiers. High-performance liquid chromatographic analysis revealed that TA levels in the brain and suboesophageal ganglion (SOG) and the OA level in brain were increased in soldiers than in pseudergates (worker caste). Immunohistochemical analysis revealed that TA/OA neurons that innervate specific areas, including the mandibular muscles, antennal nerve, central complex, suboesophageal ganglion, and thoracic and/or abdominal ganglia, were enlarged in a soldier-specific manner. Together with the results that pharmacological application of TA promoted the defensive behavior in pseudergates, these findings suggest that the increased TA/OA levels induce the higher aggressiveness and defensive behavior in termite soldiers. The projection targets of these soldier-specific enlarged TA/OA neurons may have important roles in the higher aggressiveness and defensive behavior of the termite soldiers, inducing the neuronal transition that accompanies external morphological changes.
  • 高橋悟, 奥田泰丈, 川端邦明, 川端邦明, 青沼仁志, 青沼仁志, 佐藤雄隆, 岩田健司
    Journal of Signal Processing (Web) 20 2 65‐74(J‐STAGE) - 74 信号処理学会 2016年 [査読無し][通常論文]
     
    Multiple tracking methods are widely required in biology field, in particular neuroethology and ecology fields. Biology researches have to observe target behavior with recording video and have to spend much time for analyzing video data afterward. Here we focus on fighting behavior between male crickets. Fighting behavior is one of the common behaviors in animals. Animals usually fight for resources like foods, territory, mating partner and so on. During a fight, animals modify their aggressive motivation and behavior. In order to understand how they alter their motivation and behavior, we need to analyze the detail of the time sequence of the fighting. Therefore, we propose the video image processing method for automated observation, which enables us to observe and measure the behavioral characteristics of plural crickets simultaneously. Finally, through experiments we prove the effectiveness of our method.
  • クモヒトデのロコモーションに内在する腕内協調メカニズム
    小野達也, 加納剛史, 青沼仁志, 松坂義哉, 石黒章夫
    第21回創発システム・シンポジウム講演資料集 P-08  2015年08月 [査読無し][通常論文]
  • 脚間の力学的相互作用および情報的相互作用を用いた六脚ロボットの歩行制御
    郷田 将, 大脇 大, 加納 剛史, 青沼 仁志, 石黒 章夫
    第21回創発システム・シンポジウム講演資料集 P-13  2015年08月 [査読無し][通常論文]
  • Philip L. Newland, Mesfer S. Al Ghamdi, Suleiman Sharkh, Hitoshi Aonuma, Christopher W. Jackson
    PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 282 1812 60 - 66 2015年08月 [査読有り][通常論文]
     
    Natural and anthropogenic static electric fields are commonly found in the environment and can have both beneficial and harmful effects on many animals. Here, we asked how the fruitfly responds to these fields and what the consequences of exposure are on the levels of biogenic amines in the brain. When given a choice in a Y-tube bioassay Drosophila avoided electric fields, and the greater the field strength the more likely Drosophila were to avoid it. By comparing wild-type flies, flies with wings surgically removed and vestigial winged flies we found that the presence of intact wings was necessary to produce avoidance behaviour. We also show that Coulomb forces produced by electric fields physically lift excised wings, with the smaller wings of males being raised by lower field strengths than larger female wings. An analysis of neurochemical changes in the brains showed that a suite of changes in biogenic amine levels occurs following chronic exposure. Taken together we conclude that physical movements of the wings are used by Drosophila in generating avoidance behaviour and are accompanied by changes in the levels of amines in the brain, which in turn impact on behaviour.
  • Cricket switches locomotion patterns from walking to swimming by evaluating reaction forces from the environment
    Hitoshi Aonuma, Masashi Goda, Shigeru Kuroda, Takeshi Kano, Dai Owaki, Akio Ishiguro
    Proceedings of The 7th International Symposium on Adaptive Motion of Animals and Machines (AMAM2015) 2015年06月 [査読有り][通常論文]
  • 小林充, 片岡崇, 青沼仁志, 柴田洋一
    農業食料工学会誌 77 3 179 - 185 農業食料工学会事務局 2015年05月01日 [査読無し][通常論文]
  • Aonuma H, Sakura M, Kurabayashi D
    Memory Consolidation 37 - 52 2015年 [査読有り][通常論文]
  • Ahmad M. Alessi, Vincent O'Connor, Hitoshi Aonuma, Philip L. Newland
    FRONTIERS IN BEHAVIORAL NEUROSCIENCE 8 November 371  2014年11月 [査読有り][通常論文]
     
    Phenotypic plasticity allows animals to modify their behavior, physiology, and morphology to adapt to environmental change. The global pest, the desert locust, shows two extreme phenotypes; a solitarious phase that is relatively harmless and a gregarious phase that forms swarms and causes extensive agricultural and economic damage. In the field, environmental conditions can drive isolated animals into crowded populations and previous studies have identified the biogenic amine serotonin as a key determinant of this transition. Here we take an integrated approach to investigate the neurochemical, physiological, and behavioral correlates defined by a laboratory based paradigm that mimics facets of swarm break down as gregarious locusts become isolated. Following isolation there was an increased propensity of locusts to avoid conspecifics, and show a reduced locomotion. Changes in choice behavior occurred within 1 h of isolation although isolation-related changes progressed with increased isolation time. Isolation was accompanied by changes in the levels of the biogenic amines dopamine, octopamine, and serotonin within the CNS within 1 h. Dopamine levels were higher in isolated animals and we focused on the role played by this transmitter in synaptic changes that may underpin solitarization. Dopamine reduced synaptic efficacy at a key central synapse between campaniform sensilla (CS) and a fast extensor tibiae motor neuron that is involved in limb movement. We also show that dopamine injection into the haemocoel was sufficient to induce solitarious-like behavior in otherwise gregarious locusts. Further, injection of a dopamine antagonist, fluphenazine, into isolated locusts induced gregarious-like behavior. This highlights that dopaminergic modulation plays an important role in the plasticity underpinning phase transition and sets a context to deepen the understanding of the complementary role that distinct neuromodulators play in polyphenism in locusts.
  • Mathematical Model of Inter-arm Coordination Mechanism of Ophiuroids
    Takeshi Kano, Eiki Sato, Hitoshi Aonuma, Yoshiya Matsuzaka, Akio Ishiguro
    The Joint Annual Meeting of the Japanese Society for Mathematical Biology and the Society for Mathematical Biology 予稿集 2014年07月 [査読無し][通常論文]
  • Ryuichi Okada, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma, Etsuro Ito
    SCIENTIFIC REPORTS 4 4175  2014年02月 [査読有り][通常論文]
     
    The honeybee waggle dance communicates the location of profitable food sources, usually with a certain degree of error in the directional information ranging from 10-15 degrees at the lower margin. We simulated one-day colonial foraging to address the biological significance of information error in the waggle dance. When the error was 30 degrees or larger, the waggle dance was not beneficial. If the error was 15 degrees, the waggle dance was beneficial when the food sources were scarce. When the error was 10 degrees or smaller, the waggle dance was beneficial under all the conditions tested. Our simulation also showed that precise information (0-5 degrees error) yielded great success in finding feeders, but also caused failures at finding new feeders, i.e., a high-risk high-return strategy. The observation that actual bees perform the waggle dance with an error of 10-15 degrees might reflect, at least in part, the maintenance of a successful yet risky foraging trade-off.
  • T. Watanabe, H. Aonuma
    INSECT MOLECULAR BIOLOGY 23 1 26 - 41 2014年02月 [査読有り][通常論文]
     
    Hu/ELAV-like RNA-binding proteins (RBPs) are involved in the post-transcriptional regulation of RNA metabolism including splicing, transport, translational control and turnover. The Hu/ELAV-like RBP genes are predominantly expressed in neurons, and are therefore used as common neuronal markers in many animals. Although the expression patterns and functions of the Hu/ELAV-like RBP genes have been extensively studied in the model insect Drosophila melanogaster, little is known in basal direct-developing insects. In the present study, we performed an identification and expression analysis of the found in neurons (fne) gene, an ancestral insect Hu/ELAV-like RBP gene, in the cricket Gryllus bimaculatus. Contrary to expectation that the Gryllus fne transcript would be predominantly expressed in the nervous system, expression analysis revealed that the Gryllus fne gene is expressed broadly. In addition, we discovered that alternative promoter usage directs tissue-specific and embryonic stage-dependent regulation of fne expression, and that alternative splicing contributes to the generation of diverse sets of fne transcripts. Our data provide novel insights into the evolutionary diversification of the Hu/ELAV-like RBP gene family in insects.
  • 青沼 仁志
    日本画像学会誌 = Journal of the Imaging Society of Japan 53 3 207 - 215 日本画像学会 2014年 [査読無し][通常論文]
  • Takayuki Watanabe, Hisayo Sadamoto, Hitoshi Aonuma
    INVERTEBRATE NEUROSCIENCE 13 2 107 - 123 2013年12月 [査読有り][通常論文]
     
    In insects, dopamine modulates various aspects of behavior such as learning and memory, arousal and locomotion, and is also a precursor of melanin. To elucidate the molecular basis of the dopaminergic system in the field cricket Gryllus bimaculatus DeGeer, we identified genes involved in dopamine biosynthesis, signal transduction, and dopamine re-uptake in the cricket. Complementary DNA of two isoforms of tyrosine hydroxylase (TH), which convert tyrosine into l-3,4-dihydroxyphenylalanine, was isolated from the cricket brain cDNA library. In addition, four dopamine receptor genes (Dop1, Dop2, Dop3, and DopEcR) and a high-affinity dopamine transporter gene were identified. The two TH isoforms contained isoform-specific regions in the regulatory ACT domain and showed differential expression patterns in different tissues. In addition, the dopamine receptor genes had a receptor subtype-specific distribution: the Dop1, Dop2, and DopEcR genes were broadly expressed in various tissues at differential expression levels, and the Dop3 gene was restrictedly expressed in neuronal tissues and the testicles. Our findings provide a fundamental basis for understanding the dopaminergic regulation of diverse physiological processes in the cricket.
  • Kuniaki Kawabata, Hitoshi Aonuma, Koh Hosoda, Jianru Xue
    Robotics and Autonomous Systems 61 12 1529 - 1538 2013年12月 [査読有り][通常論文]
     
    This paper describes about a prototype system for active interaction experiment to a cricket by utilizing an operated micro mobile robot. It is also able to measure/collect behavior data of the cricket on-line. The behavior selection of the cricket (Gryllus bimaculatus) is influenced by the experience or the context in living environment. In our current research, we are trying to investigate neuronal mechanisms underlying adaptive behavior switching of the cricket based on individual interactions. However in conventional biological experiments, the conditions are not maneuverable intentionally. Therefore, we are developing an integrated system for conducting active interaction experiments and gathering behavior data related to the effect of interactions. The prototype consists of a micro mobile robot as a physical interaction agent, a camera and a microphone and a computer. The computer is for commanding the robot by the operator's input. It also works for recording the data of a video sequence, on-line motion tracking and the audio during the experiment. Interaction experiments with the cricket utilizing the prototype system were done. From the results, we could confirm it works well especially for maneuvering experimental conditions and on-line experimental data gathering. © 2013 Elsevier B.V. All rights reserved.
  • Shiro Yano, Takayuki Watanabe, Hitoshi Aonuma, Hajime Asama
    Molecular BioSystems 9 8 2079 - 2084 2013年08月 [査読有り][通常論文]
     
    Abnormalities in the serotonergic system are thought to be a potent cause of several mental diseases. Past research has shown that autoreceptors are the key component. It is thought that the autoreceptor constructs a negative feedback circuit on this system and realizes homeostatic control on its neural activity. This study is mainly organized from the above mentioned knowledge. In this paper, we construct two possible models of the serotonergic system based on receptor theory and provide some predictions for this system with each model. In the first model, we predict that the deficit of serotonin synthesis causes destabilization of the amount of autoreceptors autoreceptors show an explosive increase if serotonin synthesis drops below a certain threshold value. In the second model, we indicate that the serotonergic system changes its stable property from a monostable one to a bistable one by certain factors. We clarify these factors and show that this changing process is named pitch-fork bifurcation. Additionally, we also suggest another notable phenomena which would appear when we consider a stochastic perturbation on the receptor expressions. Lastly, we suggest some experimental ideas towards the verification of the validity of these models. © 2013 The Royal Society of Chemistry.
  • Midori Sakura, Hitoshi Aonuma
    JOURNAL OF EXPERIMENTAL BIOLOGY 216 12 2221 - 2228 2013年06月 [査読有り][通常論文]
     
    Male crickets (Gryllus bimaculatus) exhibit intensively defensive aggressive behavior towards attacking males most often culminating in fighting. After the fight, the loser no longer exhibits aggressiveness in a second, separate encounter with another male; rather, the defeated male exhibits avoidance behavior. Here, we investigated the role of sensory input from the antennae in male defensive aggressive behavior. When we removed antennae from males (antennectomized males), we found that they showed little aggressiveness towards each other whereas they continued to exhibit typical fighting behavior towards an intact male. In addition, in a second encounter, antennectomized losers showed significantly higher aggressiveness towards another male than did intact losers. We further found that antennectomized crickets do not utilize visual or palpal sensory input to elicit aggressive behavior. In contrast, intact males showed aspects of aggressive behavior to male cuticular substances before and after winning a fight, and if they lost a fight they showed avoidance behavior. It thus appears that antennal sensory information is crucial in the mediation of aggressive and avoidance behaviors. However, sensory inputs from the antennae are not necessary to elicit defensive aggressive behavior but are necessary to discriminate conspecific males and initiate attacks against them.
  • Kuniaki Kawabata, Hitoshi Aonuma, Koh Hosoda, Jianru Xue
    Journal of Robotics and Mechatronics 25 2 333 - 339 2013年04月 [査読有り][通常論文]
     
    This paper describes our trials in developing automated interaction systems with crickets by using a micro mobile robot for attracting pheromone behavior. The cricket Gryllus bimaculatus modifies its behavior based on experience that is based on pheromone interactions between individuals. In developing systems, a micro mobile robot with a cricket's head is controlled based on online visual tracking information. In this paper, we describe implementing automated micro mobile robot control based on image processing and attempt experimental trials in interaction between the cricket and micro mobile robot. The cricket shows a typical response to the pheromone stimulus for approaching by the micro mobile robot. Results also show that the developed system could contribute to novel biological research, for example, manipulable experimental conditions for interactive experiments.
  • Toward realization of resilient locomotion: Lessons from the locomotion of arm-amputated ophiuroids
    Takeshi Kano, Shota Suzuki, Eiki Sato, Hitoshi Aonuma, Akio Ishiguro
    Proc. of 6th International Symposium on Adaptive Motion of Animals and Machines 2013年03月 [査読有り][通常論文]
  • Atsushi Yoritsune, Hitoshi Aonuma
    INVERTEBRATE NEUROSCIENCE 12 2 103 - 117 2012年12月 [査読有り][通常論文]
     
    Antennae are one of the major organs to detect chemo- and mechanosensory cue in crickets. Little is known how crickets process and integrate different modality of information in the brain. We thus used a number of different anatomical techniques to gain an understanding of the neural pathways extending from the antennal sensory neurons up to centers in the brain. We identified seven antennal sensory tracts (assigned as T1-7) utilizing anterograde dye filling from the antennal nerve. Tracts T1-T4 project into the antennal lobe (AL), while tracts T5 and T6 course into the dorsal region of the deutocerebrum or the suboesophageal ganglion, and finally, tract T7 terminates in the ventral area of flagellar afferent (VFA). By analyzing autofluorescence images of the AL, we identified 49 sexually isomorphic glomeruli on the basis of shape, relative position and size. On the basis of our sensory-tract data, we assigned the glomeruli into one of four separate groups. We then three-dimensionally reconstructed the internal structures in the AL (glomeruli) and the VFA (layers). Next in the protocerebrum, we identified both the tracts and their terminations from the AL and VFA. We found that 10 tracts originate in the AL, whereas there are at least eight tracts from the VFA. Several tracts from the AL share their routes with those from the VFA, but their termination areas are segregated. We now have a better anatomical understanding of the pathways for the antennal information in cricket.
  • クモヒトデのロコモーションから学ぶ大自由度系の自律分散制御 ~上位神経系の介在を考慮した腕内協調のモデル化~
    鈴木翔太, 佐藤英毅, 加納剛史, 青沼仁志, 石黒章夫
    計測自動制御学会システムインテグレーション部門 講演会予稿集(CD-ROM) 0949 - 0953 2012年12月 [査読無し][通常論文]
  • Etsuro Ito, Emi Otsuka, Noriyuki Hama, Hitoshi Aonuma, Ryuichi Okada, Dai Hatakeyama, Yutaka Fujito, Suguru Kobayashi
    PLOS ONE 7 8 e43151  2012年08月 [査読有り][通常論文]
     
    Background: The pond snail Lymnaea stagnalis can maintain a conditioned taste aversion (CTA) as a long-term memory. Previous studies have shown that the inhibitory postsynaptic potential (IPSP) evoked in the neuron 1 medial (N1M) cell by activation of the cerebral giant cell (CGC) in taste aversion-trained snails was larger and lasted longer than that in control snails. The N1M cell is one of the interneurons in the feeding central pattern generator (CPG), and the CGC is a key regulatory neuron for the feeding CPG. Methodology/Principle Findings: Previous studies have suggested that the neural circuit between the CGC and the N1M cell consists of two synaptic connections: (1) the excitatory connection from the CGC to the neuron 3 tonic (N3t) cell and (2) the inhibitory connection from the N3t cell to the N1M cell. However, because the N3t cell is too small to access consistently by electrophysiological methods, in the present study the synaptic inputs from the CGC to the N3t cell and those from the N3t cell to the N1M cell were monitored as the monosynaptic excitatory postsynaptic potential (EPSP) recorded in the large B1 and B3 motor neurons, respectively. The evoked monosynaptic EPSPs of the B1 motor neurons in the brains isolated from the taste aversion-trained snails were identical to those in the control snails, whereas the spontaneous monosynaptic EPSPs of the B3 motor neurons were significantly enlarged. Conclusion/Significance: These results suggest that, after taste aversion training, the monosynaptic inputs from the N3t cell to the following neurons including the N1M cell are specifically facilitated. That is, one of the memory traces for taste aversion remains as an increase in neurotransmitter released from the N3t cell. We thus conclude that the N3t cell suppresses the N1M cell in the feeding CPG, in response to the conditioned stimulus in Lymnaea CTA.
  • Hitoshi Aonuma, Takayuki Watanabe
    PLOS ONE 7 8 e43377  2012年08月 [査読有り][通常論文]
     
    We examined changes in the content of biogenic amines in the brains of ant queen associated with early colony establishment. In ants, including Formica japonica, winged virgin queens lose their wings following copulation, and then start establishing a colony. Significant changes in brain biogenic amine content in the queen are associated with transition from winged virgin queen to wingless mated queen. The levels of serotonin (5HT), octopamine (OA) and dopamine (DA) decreased significantly in the brain of the queen after starting a colony. On the other hand, tyramine (TA) increased significantly in the brain following colony establishment. Catabolized substances of the biogenic amines in the brain were also measured. The levels of N-acetyloctopamine (Nac-OA) and N-acetyltyramine (Nac-TA) in the brain did not show a significant change after the queen established a colony. However, the levels of N-acetylserotonin (Nac-5HT) in the brain were significantly higher in wingless mated queens than in winged virgin queens, whereas levels of N-acetyldopamine (Nac-DA) in the brain were significantly lower in wingless mated queens than winged virgin queens. These results suggest that serotonergic and octopaminergic systems in the brain of the queen change when the mated queen starts to establish a new colony.
  • Shiro Yano, Yusuke Ikemoto, Hitoshi Aonuma, Hajime Asama
    ROBOTICS AND AUTONOMOUS SYSTEMS 60 5 722 - 728 2012年05月 [査読有り][通常論文]
     
    It is thought that the adjustment of intraspecific aggression is an essential factor in the development of a social structure. To understand the natural laws for organizing the social structure, we focus on the fighting behavior of crickets, Gryllus bimaculatus, and investigate the neuronal mechanisms to adjust aggressiveness associated with a neuromodulatory biological amine: serotonin (5-HT).In this paper, we present a working theory of a neurophysiological mechanism based on the past biological studies on the 5-HT hypothesis, and a mathematical model of the mechanism. We analyzed this model and concluded that this neurophysiological mechanism makes the forgetting process slower. Next, we fitted our theoretical forgetting curve to an experimental curve and estimated the parameters of our model. These estimated values were in agreement with common belief in biological science. (C) 2011 Elsevier B.V. All rights reserved.
  • Ryuichi Okada, Tadaaki Akamatsu, Kanako Iwata, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma, Etsuro Ito
    JOURNAL OF EXPERIMENTAL BIOLOGY 215 10 1633 - 1641 2012年05月 [査読有り][通常論文]
     
    A honeybee informs her nestmates about the location of a profitable food source that she has visited by means of a waggle dance: a round dance and a figure-of-eight dance for a short- and long-distance food source, respectively. Consequently, the colony achieves an effective collection of food. However, it is still not fully understood how much effect the dance behavior has on the food collection, because most of the relevant experiments have been performed only in limited locations under limited experimental conditions. Here, we examined the efficacy of the waggle dances by physically preventing bees from dancing and then analyzing the changes in daily mass of the hive as an index of daily food collection. To eliminate place-and year-specific effects, the experiments were performed under fully natural conditions in three different cities in Japan from mid September to early October in three different years. Because the experiments were performed in autumn, all six of the tested colonies lost mass on most of the experimental days. When the dance was prevented, the daily reduction in mass change was greater than when the dance was allowed, i.e. the dance inhibited the reduction of the hive mass. This indicates that dance is effective for food collection. Furthermore, clear inhibition was observed on the first two days of the experiments; after that, inhibition was no longer evident. This result suggests that the bee colony adapted to the new environment.
  • Yuki Fukumitsu, Keiichi Irie, Tomomitsu Satho, Hitoshi Aonuma, Hamady Dieng, Abu Hassan Ahmad, Yukihiko Nakashima, Kenichi Mishima, Nobuhiro Kashige, Fumio Miake
    PARASITES & VECTORS 5 1 92  2012年05月 [査読有り][通常論文]
     
    Background: Mosquito-borne viruses are transmitted to human hosts via blood-feeding behavior of female mosquitoes. Female mosquitoes seek a host to take blood meals (host-seeking behavior). In order to prevent virus infections, it is important to understand how they modulate host-seeking behavior. Dopamine (DA) in the central nervous system acts as a neuromediator that regulates a variety of behaviors in insects. In female mosquitoes, host-seeking behavior increases when DA levels in the head decline after emergence. However, it remains unclear whether DA directly modulates host-seeking behavior in female mosquitoes. The aim of this study was to examine whether changes in DA levels in the head affects host-seeking activity in the adult female mosquito Aedes albopictus (Ae. albopictus). Findings: We compared host-seeking behavior in one group of emerging female adults treated with L-beta-3,4-dihydroxyphenylalanine (L-DOPA), the precursor of DA, (L-DOPA group), with that in an untreated control (control group) after confirming elevation of head DA in L-DOPA group by using high-performance liquid chromatography. The content of head DA in L-DOPA group significantly remained higher than that in controls on all days examined. The host-seeking activity in the control group showed a gradual increase over the 6-day experimental period. In contrast, there was no such increase in the host-seeking activity in the L-DOPA group. Therefore, the host-seeking activity of L-DOPA group was significantly lower than that of the controls between day 3 and 6 post-emergence. Conclusion: Our results indicate that elevation of DA level reduces host-seeking activity in adult female mosquito Ae. albopictus.
  • Kuniaki Kawabata, Takashi Fujii, Hitoshi Aonuma, Tsuyoshi Suzuki, Masatoshi Ashikaga, Jun Ota, Hajime Asama
    ROBOTICS AND AUTONOMOUS SYSTEMS 60 5 707 - 713 2012年05月 [査読有り][通常論文]
     
    The results of neurophysiological research have indicated the important role of neuromodulation in behavior decisions during fighting between male crickets. To understand such a dynamic behavior generation mechanism, we attempt to construct a neuromodulation model of behavior selection for the fighting behavior of a cricket. In this paper, we propose a dynamic system model of neuromodulation with the efficacy based on neurophysiological knowledge, facts, and hypotheses from the viewpoint of synthesis. The efficacy of the sensory processing center is introduced to our previous model and its dynamics is influenced by the frequency of the interactions with the other cricket. Utilizing an extended neuromodulation model, computer simulations related to a multiple-individual environment were carried out, and the results are discussed. In particular, the properties related to the standard deviation of the value of octopamine in several population density cases are discussed. (C) 2011 Elsevier B.V. All rights reserved.
  • Tatsuya Mizuno, Midori Sakura, Masatoshi Ashikaga, Hitoshi Aonuma, Ryosuke Chiba, Jun Ota
    ROBOTICS AND AUTONOMOUS SYSTEMS 60 5 700 - 706 2012年05月 [査読有り][通常論文]
     
    In order to gain better understandings how animals establish an internal mechanism, we have focused on the aggressive behavior in the male crickets, in which some different modalities of sensory inputs modify the behavioral motivation. In this research, we examined how aggressiveness changes if crickets cannot perceive normal visual inputs or antennal inputs. First, behavior of the crickets is segmented based on behavioral observation. Second, sensory input-behavior output models are selected by utilizing Minimal Description Length (MDL) criterion. Third, multi-modality sensory-behavioral experiments are realized with handicapped crickets. Last, relations between multi-modality and behavior are discussed based on the obtained parameters in the model derived with experimental results. (C) 2011 Elsevier B.V. All rights reserved.
  • Midori Sakura, Ryuichi Okada, Hitoshi Aonuma
    PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 279 1728 535 - 542 2012年02月 [査読有り][通常論文]
     
    Many insects use the polarization pattern of the sky for obtaining compass information during orientation or navigation. E-vector information is collected by a specialized area in the dorsal-most part of the compound eye, the dorsal rim area (DRA). We tested honeybees' capability of learning certain e-vector orientations by using a classical conditioning paradigm with the proboscis extension reflex. When one e-vector orientation (CS+) was associated with sugar water, while another orientation (CS-) was not rewarded, the honeybees could discriminate CS+ from CS-. Bees whose DRA was inactivated by painting did not learn CS+. When ultraviolet (UV) polarized light (350 nm) was used for CS, the bees discriminated CS+ from CS-, but no discrimination was observed in blue (442 nm) or green light (546 nm). Our data indicate that honeybees can learn and discriminate between different e-vector orientations, sensed by the UV receptors of the DRA, suggesting that bees can determine their flight direction from polarized UV skylight during foraging. Fixing the bees' heads during the experiments did not prevent learning, indicating that they use an 'instantaneous' algorithm of e-vector detection; that is, the bees do not need to actively scan the sky with their DRAs ('sequential' method) to determine e-vector orientation.
  • H. Aonuma, T. Watanabe
    ACTA BIOLOGICA HUNGARICA 63 63 - 68 2012年 [査読有り][通常論文]
     
    The ant, Formica japonica, is polyphagous and workers hunt other insects as foods. In this study, interspecific aggression was examined in the workers and queens. Behavior experiments demonstrated that interspecific aggressiveness was significantly higher in workers than queens. Workers showed predatory aggressive behavior towards crickets, on the other hand, queens elicited threat behavior but they didn't attack crickets. In order to investigate neuronal mechanisms underlying regulation of aggressive motivation, the role of biogenic amine in the brain in evoking aggressive behavior was examined by measuring biogenic amine using high-performance liquid chromatography (HPLC) with electrochemical detection (ECD). No significant difference in the octopamine (OA) level was found between workers and queens, but the level of N-acetyloctopamine (NacOA) in the brain of queens was significantly higher than that of workers. This study suggests that OAergic system in the brain must involve in controlling aggressive motivation in the ants.
  • R. Okada, H. Ikeno, T. Kimura, Mizue Ohashi, H. Aonuma, E. Ito
    ACTA BIOLOGICA HUNGARICA 63 75 - 79 2012年 [査読有り][通常論文]
     
    A honeybee informs her nestmates of the location of a flower by doing a waggle dance. The waggle dance encodes both the direction of and distance to the flower from the hive. To reveal how the waggle dance benefits the colony, we created a Markov model of bee foraging behavior and performed simulation experiments by incorporating the biological parameters that we obtained from our own observations of real bees as well as from the literature. When two feeders were each placed 400 m away from the hive in different directions, a virtual colony in which honeybees danced and correctly transferred information (a normal, real bee colony) made significantly greater numbers of successful visits to the feeders compared to a colony with inaccurate information transfer. Howerer, when five feeders were each located 400 m from the hive, the inaccurate information transfer colony performed better than the normal colony. These results suggest that dancing's ability to communicate accurate information depends on the number of feeders. Furthermore, because non-dancing colonies always made significantly fewer visits than those two colonies, we concluded that dancing behavior is beneficial for hives' ability to visit food sources.
  • Watanabe T, Aonuma H
    Acta biologica Hungarica 63 58 - 62 2012年 [査読有り][通常論文]
     
    Biogenic amine serotonin (5-HT) modulates various aspects of behaviors such as aggressive behavior and circadian behavior in the cricket. In our previous report, in order to elucidate the molecular basis of the cricket 5-HT system, we identified three genes involved in 5-HT biosynthesis, as well as four 5-HT receptor genes (5-HT1A, 5-HT1B, 5-HT2 alpha, and 5-HT7) expressed in the brain of the field cricket Gryllus bimaculatus DeGeer [7]. In the present study, we identified Gryllus 5-HT2 beta gene, an additional 5-HT receptor gene expressed in the cricket brain, and examined its tissue-specific distribution and embryonic stage-dependent expression. Gryllus 5-HT2 beta gene was ubiquitously expressed in the all examined adult tissues, and was expressed during early embryonic development, as well as during later stages. This study suggests functional differences between two 5-HT2 receptors in the cricket.
  • Midori Sakura, T. Watanabe, H. Aonuma
    ACTA BIOLOGICA HUNGARICA 63 69 - 74 2012年 [査読有り][通常論文]
     
    Aggressive behavior of white-eye mutant crickets was investigated and compared with that of wild-type crickets. In the dark, wild-type pairs performed long-lasting fights with significantly higher aggressive levels compared to those in the light. In contrast, fights between two white-eye mutants were not significantly different with those between two wild-type crickets both in duration and the aggressive levels. Ethograms of aggressive behavior showed that the mutants could show typical sequentially escalating fight with the same behavioral categories as the wild-type crickets. These results indicate that the white-eye mutants are able to express normal aggressive behavior.
  • Tetsuro Funato, Masahito Nara, Daisuke Kurabayashi, Masatoshi Ashikaga, Hitoshi Aonuma
    JOURNAL OF EXPERIMENTAL BIOLOGY 214 14 2426 - 2434 2011年07月 [査読有り][通常論文]
     
    Aggressive behaviour within pairs of male crickets leads to the establishment of a dominance hierarchy. Defeated males avoid their victorious adversaries for several hours before regaining aggressiveness. However, the defeated male does not regain aggressiveness if repeated fighting occurs. Loss of individual aggressiveness is limited by group size, which constrains the number of crickets fighting at any given time. Thus, group aggressive behaviour is modulated by an environmental factor, group size, which is ultimately determined by individual actions, i.e. fighting between two individuals. We developed a robot model to elucidate the mechanism of group-size-dependent behaviour alternation in crickets. The behaviour of individual robots was evaluated experimentally with mobile robots and the group behaviour of the robots was evaluated by computer simulation. We demonstrated that the group-size-dependent strategy in crickets could be generated by local interactions between robots, where the behaviour was governed by an oscillator and memory of the outcome of previous fights.
  • 青沼 仁志
    精神科 18 5 535 - 541 科学評論社 2011年05月 [査読無し][通常論文]
  • Ayako Wada-Katsumata, Ryohei Yamaoka, Hitoshi Aonuma
    JOURNAL OF EXPERIMENTAL BIOLOGY 214 10 1707 - 1713 2011年05月 [査読有り][通常論文]
     
    In ants, including Formica japonica, trophallaxis and grooming are typical social behaviors shared among nestmates. After depriving ants of either food or nestmates and then providing them with either food or nestmates, a behavioral change in type and frequency of social interactions was observed. We hypothesized that starvation and isolation affected levels of brain biogenic amines including dopamine (DA) and octopamine (OA) - neuromediators modifying various insect behaviors - and tested the relationship between brain biogenic amines and social behaviors of stressed ants. Ants starved for 7. days contained lower brain DA levels and they did not perform trophallaxis toward nestmates. Feeding starved ants sucrose solution re-established trophallaxis but not brain DA levels. The performance of trophallaxis induced recovery of brain DA content to the level of untreated ants. Ants that were isolated for 2 days displayed markedly increased OA levels, which following nestmate interactions, returned to levels similar to those of control (non-isolated) ants and ants isolated for 1. h. We conclude that: (1) starvation reduced brain DA level but had no significant effect on brain OA (trophallaxis recovered the brain DA levels), and (2) isolation increased brain OA level but had no effect on brain DA (trophallaxis and grooming events recovered the brain OA levels). We suggest that social interactions with nestmates influenced brain biogenic amine homeostasis in stressed F. japonica.
  • Rodrigo da Silva Guerra, Hitoshi Aonuma, Koh Hosoda, Minoru Asada
    Journal of Neuroscience Methods 191 1 138 - 144 2010年08月 [査読有り][通常論文]
     
    This paper proposes a novel robot/insect mixed society setup which enhances the possibilities for insect behavioral research and can be used as a powerful tool for interdisciplinary studies on insect behavior. Micro-robots are equipped with decoys so as to allow a controlled dynamic interaction with crickets, Gryllus bimaculatus. A camera records the interaction and the video is later processed for the automatic tracking of each encounter between cricket and robot. A novelty of our method lies in using the robots as tools for the controlled evoking of specific insect behaviors rather than trying to build an insect-like robot. The possibility for performing controlled repeatable movements allows the stimulation of certain insect behaviors that are usually difficult to trigger using insects alone, allowing consistent behavioral research. A set of experiments were performed in order to validate the proposed setup. We also demonstrate the use of our setup for stimulating agonistic behavior during an electromyography recording session. © 2010 Elsevier B.V.
  • Ryuichi Okada, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma, Etsuro Ito
    INFORMATION-AN INTERNATIONAL INTERDISCIPLINARY JOURNAL 13 3B 1115 - 1130 2010年05月 [査読有り][通常論文]
     
    It is well established that honeybees inform their nestmates of the location of flowers they have visited by a unique behavior called a "waggle dance". To evaluate the efficacy of this dance in terms of food collection, we constructed a Markov model for foraging behavior. The computer simulation showed that our model faithfully expressed actual bee behavior; revealed that a colony in which honeybees danced and correctly transferred information made 2.15 times more successful visits to food sources compared to a colony with non-accurate information transfer; and suggested that the waggle dance is effective only under particular conditions.
  • Shiro Yano, Yusuke Ikemoto, Hitoshi Aonuma, Takashi Nagao, Hajime Asama
    DISTRIBUTED AUTONOMOUS ROBOTIC SYSTEMS 8 97 - + 2009年 [査読有り][通常論文]
     
    Larval crickets develop a big body and high aggressiveness under isolated conditions, but develop a small body and low aggression under crowded conditions. Furthermore, their ensemble variance increases. It is known that the heavier cricket tends to win. Therefore, the two growth processes affect competition. We constructed a mathematical model for intraspecific competition based on the Bonabeau model, showing body weight effects on the winning rate. Results show that the population mean of body weight decreases under the condition of specific memory enhancement that places more emphasis on losing than winning. Furthermore, hierarchical effects in foraging efficiency increase the ensemble variance.
  • Shiro Yano, Yusuke Ikemoto, Hitoshi Aonuma, Takashi Nagao, Hajime Asama
    DISTRIBUTED AUTONOMOUS ROBOTIC SYSTEMS 8 97 - + 2009年 [査読有り][通常論文]
     
    Larval crickets develop a big body and high aggressiveness under isolated conditions, but develop a small body and low aggression under crowded conditions. Furthermore, their ensemble variance increases. It is known that the heavier cricket tends to win. Therefore, the two growth processes affect competition. We constructed a mathematical model for intraspecific competition based on the Bonabeau model, showing body weight effects on the winning rate. Results show that the population mean of body weight decreases under the condition of specific memory enhancement that places more emphasis on losing than winning. Furthermore, hierarchical effects in foraging efficiency increase the ensemble variance.
  • Masatoshi Ashikaga, Midori Sakura, Mika Kikuchi, Tetsutaro Hiraguchi, Ryosuke Chiba, Hitoshi Aonuma, Jun Ota
    ADVANCED ROBOTICS 23 5 563 - 578 2009年 [査読有り][通常論文]
     
    Agonistic behavior in crickets was investigated to understand socially adaptive behavior, which assists with the understanding of the design of an artificial autonomous system in a social organization. Agonistic behavior between male crickets is released by the perception of cuticular substances of conspecific males. The degree of aggressiveness in crickets escalates from antennal fencing to tactical contact until one male quits fighting, at which time social status is established. The question of whether crickets are able to recognize one another to establish social status in an agonist interaction is worthy of pursuit. However, it would be difficult to conduct an experiment to explore this issue while using many individuals. Hence, we examined the social organization among male crickets using a two-step approach: (i) an experiment involving a simulated cricket behavior model and (ii) an experiment involving a behavior experiment using real animals. Our results suggest that crickets establish social status without recognition of their opponents. (c) Koninklijke Brill NV, Leiden and The Robotics Society of Japan, 2009
  • Madoka Yamazaki, Midori Sakura, Hitoshi Aonuma, Toshiharu Akino, Ryohei Yamaoka
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 151 4 453 - 453 2008年12月 [査読有り][通常論文]
  • Ryuichi Okada, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma, Etsuro Ito
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 151 4 456 - 456 2008年12月 [査読有り][通常論文]
  • Modeling a Behavior Selection Mechanism of Insects via Structural Transition of Oscillator Networks
    Tetsuro Funato, Masahito Nara, Daisuke Kurabayashi, Hitoshi Aonuma, Masatoshi Ashikaga
    Mobiligence workship in IROS2008 11  2008年09月 [査読有り][通常論文]
  • Yuki Ishikawa, Hitoshi Aonuma, Toru Miura
    PLOS ONE 3 7 e2617 - 8 2008年07月 [査読有り][通常論文]
     
    Social insects exhibit a variety of caste-specific behavioral tendencies that constitute the basis of division of labor within the colony. In termites, the soldier caste display distinctive defense behaviors, such as aggressively attacking enemies with well-developed mandibles, while the other castes retreat into the colony without exhibiting any aggressive response. It is thus likely that some form of soldier-specific neuronal modification exists in termites. In this study, the authors compared the brain (cerebral ganglion) and the suboesophageal ganglion (SOG) of soldiers and pseudergates (workers) in the damp-wood termite, Hodotermopsis sjostedti. The size of the SOG was significantly larger in soldiers than in pseudergates, but no difference in brain size was apparent between castes. Furthermore, mandibular nerves were thicker in soldiers than in pseudergates. Retrograde staining revealed that the somata sizes of the mandibular motor neurons (MdMNs) in soldiers were more than twice as large as those of pseudergates. The enlargement of MdMNs was also observed in individuals treated with a juvenile hormone analogue (JHA), indicating that MdMNs become enlarged in response to juvenile hormone (JH) action during soldier differentiation. This enlargement is likely to have two functions: a behavioral function in which soldier termites will be able to defend more effectively through relatively faster and stronger mandibular movements, and a developmental function that associates with the development of soldier-specific mandibular muscle morphogenesis in termite head. The soldier-specific enlargement of mandibular motor neurons was observed in all examined species in five termite families that have different mechanisms of defense, suggesting that such neuronal modification was already present in the common ancestor of termites and is significant for soldier function.
  • Tetsuro Funato, Daisuke Kurabayashi, Masahito Nara, Hitoshi Aonuma
    IEEE TRANSACTIONS ON SYSTEMS MAN AND CYBERNETICS PART B-CYBERNETICS 38 3 764 - 770 2008年06月 [査読有り][通常論文]
     
    Insects have small brains, but their behavior is highly adaptive; this leads us to conclude that their brains possess a simple adaptation mechanism. This paper focuses on the pheromone processing of crickets, varying their aggression depending on their global neural connection, and proposes a behavior selection mechanism that can be controlled by network transformation. The controller is composed of an oscillator network, and its behavior is decided by the synchrony of organic oscillations. Furthermore, every network component corresponds to a certain brain module. A model is realized by using an analog circuit, and it is applied to a simple robot that displays the behavior of a real insect.
  • Oak Yono, Hitoshi Aonuma
    ZOOLOGICAL SCIENCE 25 5 517 - 525 2008年05月 [査読有り][通常論文]
     
    Crickets respond to air currents with quick avoidance behavior. The terminal abdominal ganglion (TAG) has a neuronal circuit for a wind-detection system to elicit this behavior. We investigated neuronal transmission from cercal sensory afferent neurons to ascending giant interneurons (GIs). Pharmacological treatment with 500 mu M acetylcholine (ACh) increased neuronal activities of ascending interneurons with cell bodies located in the TAG. The effects of ACh antagonists on the activities of identified GIs were examined. The muscarinic ACh antagonist atropine at 3-mM concentration had no obvious effect on the activities of GIs 10-3, 10-2, or 9-3. On the other hand, a 3-mM concentration of the nicotinic ACh antagonist mecamylamine decreased spike firing of these interneurons. Immunohistochemistry using a polyclonal anti-conjugated acetylcholine antibody revealed the distribution of cholinergic neurons in the TAG. The cercal sensory afferent neurons running through the cercal nerve root showed cholinergic immunoreactivity, and the cholinergic immunoreactive region in the neuropil overlapped with the terminal arborizations of the cercal sensory afferent neurons. Cell bodies in the median region of the TAG also showed cholinergic immunoreactivity. This indicates that not only sensory afferent neurons but also other neurons that have cell bodies in the TAG could use ACh as a neurotransmitter.
  • Hidetoshi Ikeno, Ryohei Kanzaki, Hitoshi Aonuma, Masakazu Takahata, Makoto Mizunarni, Kouji Yasuyama, Nobuyuki Matsui, Fumio Yokohari, Shiro Usui
    NEURAL INFORMATION PROCESSING, PART II 4985 PART 2 905 - + 2008年 [査読有り][通常論文]
     
    Various kinds of analysis and mathematical models based on neuroscience are developing in the neural network study. In the research, experimental data and knowledge so far obtained are essential resources to deepen their consideration of neuronal systems and functions. In order to utilize the accumulation of expertise and research effectively, it is important to integrate various resources, such as bibliography and experimental data. The managing and sharing of research resources are absolutely imperative for future development in both experimental and analytical studies. Various scientific fields need a new method to obtain precise information, because the amount of experimental data and publications has increased rapidly due to innovations in measurement, computers and network technologies. Under this situation, an effective resource-managing based on CMS (Content Management System) is introduced here for the laboratory use. We are providing functional modules to manage research resources for neuroinfomatics. As a practical use of these modules, a database system for managing image data of invertebrate neurons measured by Confocal Laser Scanning Microscope (CLSM) is developed. Furthermore, these modules are implemented for management of contents in the Invertebrate Brain Platform.
  • Ryuichi Okada, Hidetoshi Ikeno, Hitoshi Aonuma, Etsuro Ito
    ADVANCED ROBOTICS 22 15 1665 - 1681 2008年 [査読有り][通常論文]
     
    A honeybee informs her nestmates of flower locations by a unique behavior called a 'waggle dance'. We regard this behavior as a good model of the 'propagation and sharing of knowledge' to maintain a society. We have attempted to reveal how this dance benefits the colony using mathematical models and computer simulation based on parameters obtained from observations of bee behavior. Our simulation indicated that the most successful forages were made by a putative bee colony that used the dance to communicate. Video analysis of worker honeybee behavior in the field showed that a bee does not dance in a single, random place in the hive, but waggles several times in one place and several times in another. The orientation and duration of waggle runs varied from run to run, within ranges of +/- 15 degrees and +/- 15%, respectively. We also found that most of the bees that listened to the waggle dance turned away from the dancer after listening to one or two runs. These data suggest that honeybees use the waggle dance as a method of communication, but that they must base their forages oil ambiguous information about the location of a food source. (C) Koninklijke Brill NV, Leiden and The Robotics Society of Japan, 2008
  • 青沼 仁志, 長尾 隆司, 太田 順, 川端 邦明, 淺間 一
    計測と制御 = Journal of the Society of Instrument and Control Engineers 46 12 903 - 909 計測自動制御学会 2007年12月10日 [査読無し][通常論文]
  • Hiroto Ogawa, Yoshichika Baba, Yoshiichiro Kitamura, Yoshichiro Kitamura, Hitoshi Aonuma, Kotaro Oka
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 148 3 348 - 348 2007年11月 [査読有り][通常論文]
  • Dai Hatakeyama, Hitoshi Aonuma, Etsuro Ito, Karoly Elekes
    BIOLOGICAL BULLETIN 213 2 172 - 186 2007年10月 [査読有り][通常論文]
     
    We investigated the distribution and projection patterns of central and peripheral glutamate-like immunoreactive (GLU-LIR) neurons in the adult and developing nervous system of Lymnaea. Altogether, 50-60 GLU-LIR neurons are present in the adult central nervous system. GLU-LIR labeling is shown in the interganglionic bundle system and at the varicosities in neuropil of the central ganglia. In the periphery, the foot, lip, and tentacle contain numerous GLU-LIR bipolar sensory neurons. In the juvenile Lymnaea, GLU-LIR elements at the periphery display a pattern of distribution similar to that seen in adults, whereas labeled neurons increase in number in the different ganglia of the central nervous system from juvenile stage P1 up to adulthood. During embryogenesis, GLU-LIR innervation can be detected first at the 50% stage of embryonic development (the E50% stage) in the neuropil of the cerebral and pedal ganglia, followed by the emergence of labeled pedal nerve roots at the E75% stage. Before hatching, at the E90% stage, a few GLU-LIR sensory cells can be found in the caudal foot region. Our findings indicate a wide range of occurrence and a broad role for glutamate in the gastropod nervous system; hence they provide a basis for future studies on glutamatergic events in networks underlying different behaviors.
  • Masazumi Iwasaki, Hiroshi Nishino, Antonia Delago, Hitoshi Aonuma
    ZOOLOGICAL SCIENCE 24 9 860 - 868 2007年09月 [査読有り][通常論文]
     
    After a loss against an opponent, the aggressiveness of a male cricket is significantly reduced for up to 30 minutes. This depression of aggressiveness is an important factor in the establishment and maintenance of dominance between individuals. In the present study, we investigated the functional roles of nitric oxide (NO) signaling in the depression of aggressiveness in subordinate male crickets. Pairs of male crickets, pre-injected with various NO-related reagents, were allowed to establish dominant/subordinate relationships in dyadic encounters. Opponents were separated for 15 minutes and then paired again. In second encounters, subordinate crickets pre-injected with PTIO (NO scavenger) showed agonistic behavior towards former dominant opponents. A similar effect was observed in crickets pre-injected with L-NAME (NO synthase inhibitor) or ODQ (soluble guanylate cyclase inhibitor). The effects of the latter two drugs were canceled by co-injection of NOR3 (NO donor) with L-NAME or by co-injection of 8-Br-cGMP (cGMP-analog) with ODQ. Injection of NOR3 alone prolonged the inhibition of agonistic behavior in subordinate crickets from 30 minutes to 3 hours. Our results suggest that the change in agonistic behavior observed in subordinate male crickets is closely linked to NO-mediated cGMP signaling.
  • Eriko Tsuji, Hitoshi Aonuma, Fumio Yokohari, Michiko Nishikawa
    ZOOLOGICAL SCIENCE 24 8 836 - 849 2007年08月 [査読有り][通常論文]
     
    Social Hymenoptera such as ants or honeybees are known for their extensive behavioral repertories and plasticity. Neurons containing biogenic amines appear to play a major role in controlling behavioral plasticity in these insects. Here we describe the morphology of prominent serotonin-immunoreactive neurons of the antennal sensory system in the brain of an ant, Camponotus japonicus. Immunoreactive fibers were distributed throughout the brain and the subesophageal ganglion (SOG). The complete profile of a calycal input neuron was identified. The soma and dendritic elements are contralaterally located in the lateral protocerebrum. The neuron supplies varicose axon terminals in the lip regions of the calyces of the mushroom body, axon collaterals in the basal ring but not in the collar region, and other axon terminals ipsilaterally in the lateral protocerebrum. A giant neuron innervating the antennal lobe has varicose axon terminals in most of 300 glomeruli in the ventral region of the antennal lobe (AL) and a thick neurite that spans the entire SOG and continues towards the thoracic ganglia. However, neither a soma nor a dendritic element of this neuron was found in the brain or the SOG. A deutocerebral projection neuron has a soma in the lateral cell-body group of the AL, neuronal branches at most of the 12 glomeruli in the dorsocentral region of the ipsilateral AL, and varicose terminal arborizations in both hemispheres of the protocerebrum. Based on the present results, tentative subdivisions in neuropills related to the antennal sensory system of the ant brain are discussed.
  • Swidbert R. Ott, Hitoshi Aonuma, Philip L. Newland, Maurice R. Elphick
    JOURNAL OF COMPARATIVE NEUROLOGY 501 3 381 - 399 2007年03月 [査読有り][通常論文]
     
    Nitric oxide (NO) is a diffusible signaling molecule with evolutionarily conserved roles in neural plasticity. Prominent expression of NO synthase (NOS) in the primary olfactory centers of mammals and insects lead to the notion of a special role for NO in olfaction. In insects, however, NOS is also strongly expressed in non-olfactory chemo-tactile centers of the thoracic nerve cord. The functional significance of this apparent association with various sensory centers is unclear, as is. the extent to which it occurs in other arthropods. We therefore investigated the expression of NOS in the pereopod ganglia of crayfish (Pacifastacus lenisculus and Procambarus clarkii). Conventional NADPH diaphorase (NADPHd) staining after formaldehyde fixation gave poor anatomic detail, whereas fixation in methanol/formalin (MF-NADPHd) resulted in Golgi-like staining, which was supported by immunohistochemistry using NOS antibodies that recognize a 135-kDa protein in crayfish. MF-NADPHd revealed an exceedingly dense innervation of the chemo-tactile centers. As in insects, this innervation was provided by a system of prominent intersegmental neurons. Superimposed on a putatively conserved architecture, however, were pronounced segmental differences. Strong expression occurred only in the anterior three pereopod ganglia, correlating with the presence of claws on pereopods one to three. These clawed pereopods, in addition to their role in locomotion, are crucially involved in feeding, where they serve both sensory and motor functions. Our findings indicate that strong expression of NOS is not a universal feature of primary sensory centers but instead may subserve a specific requirement for sensory plasticity that arises only in particular behavioral contexts.
  • Takayuki Watanabe, Mika Kikuchi, Dai Hatakeyama, Takumi Shiga, Takehiro Yamamoto, Hitoshi Aonuma, Masakazu Takahata, Norio Suzuki, Etsuro Ito
    DEVELOPMENTAL NEUROBIOLOGY 67 4 456 - 473 2007年03月 [査読有り][通常論文]
     
    Nitric oxide (NO), hydrogen sulfide (H2S), and carbon monoxide (CO) are thought to act as gaseous neuromodulators in the brain across species. For example, in the brain of honeybee Apis mellifera, NO plays important roles in olfactory learning and discrimination, but the existence of H2S- and CO-mediated signaling pathways remains unknown. In the present study, we identified the genes of nitric oxide synthase (NOS), soluble guanylyl cyclase (sGC), cystathionine beta-synthase (CBS), and heme oxygenase (HO) from the honeybee brain. The honeybee brain contains at least one gene for each of NOS, CBS, and HO. The deduced proteins for NOS, CBS, and HO are thought to contain domains to generate NO, H2S, and CO, respectively, and to contain putative Ca2+/calmodulinbinding domains. On the other hand, the honeybee brain contains three subunits of sGC: sGC alpha 1, sGC beta 1, and sGC beta 3. Phylogenetic analysis of sGC revealed that Apis sGC alpha 1 and sGC beta 1 are closely related to NO- and CO-sensitive sGC subunits, whereas Apis sGC beta 3 is closely related to insect O-2-sensitive sGC subunits. In addition, we performed in situ hybridization for Apis NOS mRNA and NADPH-diaphorase histochemistry in the honeybee brain. The NOS gene was strongly expressed in the optic lobes and in the Kenyon cells of the mushroom bodies. NOS activity was detected in the optic lobes, the mushroom bodies, the central body complex, the lateral protocerebral lobes, and the antennal lobes. These findings suggest that NO is involved in various brain functions and that H2S and CO can be endogenously produced in the honeybee brain. (c) 2007 Wiley Periodicals, Inc.
  • Midori Sakura, Tetsutaro Hiraguchi, Kyohsuke Ohkawara, Hitoshi Aonuma
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 145 3-4 419 - 419 2006年11月 [査読有り][通常論文]
  • Akiko Wagatsuma, Sachiyo Azami, Midori Sakura, Dai Hatakeyama, Hitoshi Aonuma, Etsuro Ito
    JOURNAL OF NEUROSCIENCE RESEARCH 84 5 954 - 960 2006年10月 [査読有り][通常論文]
     
    Interaction between the activator type of cyclic AMP response element binding protein (CREB1) and the repressor type (CREB2) results in determining the emergence of long-lasting synaptic enhancement involved in memory consolidation. However, we still do not know whether the constitutively expressed forms of CREB are enough or the newly synthesized forms are required for the synaptic enhancement. In addition, if the newly synthesized forms are needed, we must determine the time for translation of CREB from its mRNA. We applied the methods of RNA interference and real-time polymerase chain reaction (PCR) to CREB in the cerebral giant cells of Lymnaea. The cerebral giant cells play an important role in associative learning and employ a CREB cascade for the synaptic enhancement to neurons such as the B1 moto-neurons. We injected the small interfering RNA (siRNA) of CREB1 or CREB2 into the cerebral giant cells and examined the changes in amplitude of excitatory postsynaptic potential (EPSP) recorded in the B1 motoneurons. The changes in the amounts of CREB1 and CREB2 mRNAs were also examined in the cerebral giant cells. The EPSP amplitude was suppressed 15 min after injection of CREB1 siRNA, whereas that was augmented 60 min after injection of CREB2 siRNA. In the latter case, the decrease in the amount of CREB2 mRNA was confirmed by real-time PCR. Our results showed that the de novo synthesized forms of CREB are required within tens of minutes for the synaptic enhancement in memory consolidation. (c) 2006 Wiley-Liss, Inc.
  • Masazumi Iwasaki, Antonia Delago, Hiroshi Nishino, Hitoshi Aonuma
    ZOOLOGICAL SCIENCE 23 10 863 - 872 2006年10月 [査読有り][通常論文]
     
    Male solitary animals frequently enter aggressive interactions with conspecific individuals to protect their territory or to gain access to females. After an agonistic encounter, the loser (subordinate individual) changes its behaviour from aggression to avoidance. We investigated agonistic interactions between pairs of male crickets to understand how dominance is established and maintained. Two naive males readily entered into agonistic interactions. Fights escalated in a stereotyped manner and were concluded with the establishment of dominance. If individuals were isolated after the first encounter and placed together 15 minutes later, subordinate crickets tended to avoid any further contact with the former dominant opponent. Moreover, subordinate males also avoided unfamiliar dominant and naive opponents. They displayed aggressive behaviour only towards unfamiliar subordinate opponents. This suggests that the subordinate male change their behaviour depending on the dominance status of the opponent. Dominant crickets, in contrast, displayed aggressive behaviour towards familiar as well as unfamiliar opponents. If the interval between the first and second encounter was longer than 30 minutes, the former subordinate male showed aggressive behaviour again. However, if the subordinate cricket was paired with the same opponent three consecutive times within 45 minutes, it avoided the former dominant opponent for up to 6 hours following the third encounter. Our results suggest that the maintenance of dominance in male crickets depends largely on the behavioural change of subordinate individuals. Possible mechanisms to maintain dominance are discussed.
  • Antonia Delago, Hitoshi Aonuma
    ZOOLOGICAL SCIENCE 23 9 775 - 783 2006年09月 [査読有り][通常論文]
     
    Fighting behavior in male crickets is already well described, and some of the mechanisms underlying aggression and aggressive motivation have already been revealed. Much less is known about female/female interactions. Here, we report that adult female crickets that had been isolated for several days readily entered into agonistic interactions with conspecific individuals. Characteristic dyadic encounters between isolated females escalated in a stepwise manner and were concluded with the establishment of a dominant/subordinate relationship. For 15 to 30 minutes following an initial fight, former subordinate females showed a dramatic change in agonistic behavior. If they were paired with the former dominant opponent during this interval, a significant majority did not enter into any aggressive interaction but instead actively avoided the opponent. A similar experience-based and time-dependent increase in avoidance was observed when former subordinate females were paired with unfamiliar naive opponents. However, when faced with an unfamiliar subordinate individual in the second encounter, no such increase in avoidance behavior was observed. We propose that the observed changes in the behavior of former subordinate females are the consequence of a change in the general state of arousal and of the recognition of dominance status, but not of individual recognition. The fact that former dominant individuals did not show similar experience-based changes in agonistic behavior suggests that dominant/subordinate relationships between pairs of female crickets are maintained mainly by the behavior of subordinate individuals.
  • Development of oscillator network model for behavioral processing.
    Tetsuro Funato, Hitoshi Aonuma, Daisuke Kurabayashi, Masahito Nara
    2nd International Workshop of Research Group of Invertebrate Nervous System 8  2006年08月 [査読有り][通常論文]
  • K Niwa, J Sakai, T Karino, H Aonuma, T Watanabe, T Ohyama, O Inanami, M Kuwabara
    FREE RADICAL RESEARCH 40 2 167 - 174 2006年02月 [査読有り][通常論文]
     
    To elucidate the role of shear stress in fluid-phase endocytosis of vascular endothelial cells (EC), we used a rotating-disk shearing apparatus to investigate the effects of shear stress on the uptake of lucifer yellow (LY) by cultured bovine aortic endothelial cells ( BAEC). Exposure of EC to shear stress (area-mean value of 10 dynes/cm(2)) caused an increase in LY uptake that was abrogated by the antioxidant, N-acetyl-L-cysteine (NAC), the NADPH oxidase inhibitor, acetovanillone, and two inhibitors of protein kinase C (PKC), calphostin C and GF109203X. These results suggest that fluid-phase endocytosis is regulated by both reactive oxygen species (ROS) and PKC. Shear stress increased both ROS production and PKC activity in EC, and the increase in ROS was unaffected by calphostin C or GF109203X, whereas the activation of PKC was reduced by NAC and acetovanillone. We conclude that shear stress-induced increase in fluid-phase endocytosis is mediated via ROS generation followed by PKC activation in EC.
  • Y Matsumoto, S Unoki, H Aonuma, M Mizunami
    LEARNING & MEMORY 13 1 35 - 44 2006年01月 [査読有り][通常論文]
     
    Cyclic AMP pathway plays ail essential role in formation of long-term memory (LTM). In some species, the nitric oxide (NO)-cyclic GMP pathway has been found to act in parallel and complementary to the cAMP pathway for LTM formation. Here we describe a new role of the NO-cGMP pathway, namely, stimulation of the cAMP pathway to induce LTM. We have studied the signaling cascade Underlying LTM formation by systematically coinjecting various "LTM-inducing" and "LTM-blocking" drugs in crickets. Multiple-trial olfactory conditioning led to LTM that lasted for several days, while memory induced by single-trial conditioning decayed away within several hours. Injection of inhibitors of the enzyme forming NO, cGMP, or cAMP into the hemolymph prior to multiple-trial conditioning blocked LTM, whereas injection of ail NO donor, cGMP analog, or cAMP analog prior to single-trial conditioning induced LTM. Induction of LTM by injection of an NO donor or cGMP analog paired with single-trial conditioning was blocked by inhibitors of the cAMP pathway, but induction of LTM by a cAMP analog was unaffected by inhibitors of the NO-cGMP pathway. Inhibitors of cyclic nucleotide-gated channel (CNG channel) or calmodulin-blocked induction of LTM by cGMP analog paired with single-trial conditioning, but they did not affect induction of LTM by cAMP analog. Our findings suggest that the cAMP pathway is a downstream target of the NO-cGMP pathway for the formation of LTM, and that the CNG channel and calcium-calmodulin intervene between the NO-cGMP pathway and the cAMP pathway.
  • J Nagamoto, H Aonuma, M Hisada
    ZOOLOGICAL SCIENCE 22 10 1079 - 1088 2005年10月 [査読有り][通常論文]
     
    Cuticular substances on the body surface of crickets serve as pheromones that elicit a variety of different behaviors in male crickets. Antennal contact between males and females resulted in courtship behavior, and that between two males resulted in aggressive displays. As a first step in elucidating how crickets recognize and discriminate individuals, behavioral responses of male individuals to cuticular substances of conspecific males or females were investigated. The behavioral responses of males to antennal or palpal stimulation with an isolated antenna from a male or a female were recorded. To both antennal and palpal stimulation with female antennae, the majority of males responded with courtship behavior; to stimulation with male antennae, males responded with aggressive displays. To gain insight into the chemical nature of the behaviorally relevant components, isolated antennae were washed in either n-hexane, acetone or ethanol before behavior assays. Washed antennae no longer elicited courtship or aggressive responses in males. Next, polypropylene fibers were smeared with substances from the body surface of females and used for antennal stimulation. This experiment showed that the quality and quantity of cuticular substances appear to be highly age-dependent. Significantly more males responded with courtship behavior to cuticular substances from younger females. Isolated males generally showed higher levels of aggression than males reared in groups. Grouped males also were more likely to display courtship behavior towards antennae from younger females, and aggressive behavior towards antennae from older females. These results suggest that male discrimination of mating partners depends on the nature of female cuticular substances.
  • 青沼仁志
    計測と制御 44 9 621 - 627 公益社団法人 計測自動制御学会 2005年09月10日 [査読無し][通常論文]
  • Y Seki, H Aonuma, R Kanzaki
    JOURNAL OF COMPARATIVE NEUROLOGY 481 4 340 - 351 2005年01月 [査読有り][通常論文]
     
    The antennal lobe (AL) of the male silkworm moth Bombyx mori contains 60 +/- 2 ventrally located antennal glomeruli and a dorsal macroglomerular complex (MGC) consisting of three subdivisions. The response patterns of MGC projection neurons (PNs) to pheromonal stimuli correlate with their dendritic arborization in the subdivisions of the MGC. However, the representation of this pheromonal information in the lateral protocerebrum. (LPC), which is the target site of the AL PNs, is not well known. We performed nitric oxide (NO)-induced anti-cGMP immunohistochemistry and found that the PNs which respond to the major pheromone component (bombykol) express strong immunoreactivity. They project to a specific area, the delta area in the inferior lateral protocerebrum (DeltaILPC), which clearly represents the processing center for the major pheromone component. Furthermore, to examine the projection sites in the LPC from each subdivision of the MGC, we performed double-labeling of each type of MGC-PNs, combined with NO-induced anti-cGMP immunohistochemistry. We revealed that projections from each subdivision of the MGC overlapped or separated in specific regions of the DeltaILPC. These results suggest that integration and segregation of pheromone information may occur in the DeltaILPC. (C) 2004 Wiley-Liss, Inc.
  • T Nagayama, KI Kimura, M Araki, H Aonuma, PL Newland
    JOURNAL OF COMPARATIVE NEUROLOGY 474 1 123 - 135 2004年06月 [査読有り][通常論文]
     
    Using an antiserum directed against glutamate, we have analyzed the distribution of glutamate-like immunoreactive neurons in the terminal abdominal ganglion of the crayfish Procambarus clarkii. Approximately 160 central neurons (157 +/- 8; mean +/- SEM, n = 8) showed positive glutamate-like immunoreactivity, which represents approximately 25% of the total number of neurons in the terminal ganglion. Using a combination of intracellular staining with the marker Lucifer yellow and immunocytochemical staining has shown that most excitatory motor neurons are glutamatergic and that glutamate acts as an excitatory transmitter at peripheral neuromuscular junctions. Seven of 10 identified spiking local interneurons and only 2 of 19 identified ascending interneurons, showed positive immunoreactivity. Our observation that inhibitory spiking interneurons were immunopositive, whereas excitatory ascending interneurons were immunonegative, indicates that glutamate is likely to act as an inhibitory neurotransmitter within the central nervous system. Local pressure injection of L-glutamate into the neuropil of the ganglion caused a hyperpolarization of the membrane potentials of many interneurons. gamma-Aminobutyric acid (GABA)ergic posterolateral nonspiking interneurons and the bilateral nonspiking interneuron LDS showed no glutamate-like immunoreactivity, whereas non-GABAergic anterolateral III nonspiking interneurons showed glutamate-like immunoreactivity. Thus, not only GABA but also glutamate are used in parallel as inhibitory neurotransmitters at central synapses. (C) 2004 Wiley-Liss, Inc.
  • H Sadamoto, H Sato, S Kobayashi, J Murakami, H Aonuma, H Ando, Y Fujito, K Hamano, M Awaji, K Lukowiak, A Urano, E Ito
    JOURNAL OF NEUROBIOLOGY 58 4 455 - 466 2004年03月 [査読有り][通常論文]
     
    The pond snail Lymnaea stagnalis is an excellent model system in which to study the neuronal and molecular substrates of associative learning and its consolidation into long-term memory. Until now, the presence of cyclic AMP (cAMP)-responsive element binding protein (CREB), which is believed to be a necessary component in the process of a learned behavior that is consolidated into long-term memory, has only been assumed in Lymnaea neurons. We therefore cloned and analyzed the cDNA sequences of homologues of CREB1 and CREB2 and determined the presence of these mRNAs in identifiable neurons of the central nervous system (CNS) of L stagnalis. The deduced amino acid sequence of Lymnaea CREB1 is homologous to transcriptional activators, mammalian CREB1 and Aplysia CREB1a, in the C-terminal DNA binding (bZIP) and phosphorylation domains, whereas the deduced amino acid sequence of Lymnaea CREB2 is homologous to transcriptional repressors, human CREB2, mouse activating transcription factor-4, and Aplysia CREB2 in the bZIP domain. In situ hybridization revealed that only a relatively few neurons showed strongly positive signals for Lymnaea CREB1 mRNA, whereas all the neurons in the CNS contained Lymnaea CREB2 mRNA. Using one of the neurons (the cerebral giant cell) containing Lymnaea CREB1 mRNA, we showed that the injection of a CRE oligonucleotide inhibited a cAMP-induced, long-lasting synaptic plasticity. We therefore conclude that CREBs are present in Lymnaea neurons and may function as necessary players in behavioral plasticity. (C) 2003 Wiley Periodicals, Inc.
  • H Schuppe, M Araki, H Aonuma, T Nagayama, PL Newland
    ZOOLOGICAL SCIENCE 21 1 1 - 5 2004年01月 [査読有り][通常論文]
     
    We have analysed the effects of the neuromodulator nitric oxide (NO) on proprioceptive information processing by ascending intersegmental interneurons that form part of the local circuits within the terminal abdominal ganglion of the crayfish. NO modulates the synaptic inputs to ascending interneurons, enhancing the amplitude of class I interneurons and reducing the amplitude of class 11 interneurons. Repetitive proprioceptive stimulation leads to rapid depression in a specific set of identified interneurons but not in others. Bath application of a nitric oxide scavenger, PTIO, causes a significant decrease in the rate of depression of the interneurons showing a rapid depression, independent of interneuron class, but has no effect on the dynamic responses of the interneurons that show little initial depression. These results indicate that NO exerts multiple effects at the very first stage of synaptic integration in local circuits.
  • H Aonuma, K Niwa
    ACTA BIOLOGICA HUNGARICA 55 1-4 65 - 70 2004年 [査読有り][通常論文]
     
    Cricket brains were incubated in a saline containing nitric oxide (NO)-donor and phosphodiesterase inhibitor IBMX, which could activate soluble guanylate cyclase (sGC) to increase cGMP levels in the targets of NO. The increase of cGMP was detected by immunohistochemistry and enzyme linked immunosorbent assay. NO-induced cGMP immunohistochemistry revealed that many cell bodies of cricket brain showed cGMP immunoreactivity when preparations were treated with a saline containing 10 mM NO-donor SNP and phosphodiesterase inhibitor IBMX, but only a few cell bodies showed immunoreactivity when preparations were incubated without NO-donor. The concentration of cGMP in cricket brains were then measured by using cGMP-specific enzyme linked immunosorbent assay. Cricket brains were treated with a saline containing 1 muM of NO-donor NOR3 and I mM IBMX. The cGMP levels in the brain were increased about 75% compared to control preparations that was treated with a cricket saline containing IBMX The level of cGMP decreased about 40% when preparations were incubated NOR3 saline containing sGC inhibitor ODQ. These results indicate that NO activates sGC and increases the levels of cGMP in particular neurons of the cricket brain and that the level of cGMP would be kept a particular level, which might regulate synaptic efficacy in the neurotransmission.
  • H Schuppe, M Araki, H Aonuma, T Nagayama, PL Newland
    ZOOLOGICAL SCIENCE 21 1 1 - 5 2004年01月 [査読有り][通常論文]
     
    We have analysed the effects of the neuromodulator nitric oxide (NO) on proprioceptive information processing by ascending intersegmental interneurons that form part of the local circuits within the terminal abdominal ganglion of the crayfish. NO modulates the synaptic inputs to ascending interneurons, enhancing the amplitude of class I interneurons and reducing the amplitude of class 11 interneurons. Repetitive proprioceptive stimulation leads to rapid depression in a specific set of identified interneurons but not in others. Bath application of a nitric oxide scavenger, PTIO, causes a significant decrease in the rate of depression of the interneurons showing a rapid depression, independent of interneuron class, but has no effect on the dynamic responses of the interneurons that show little initial depression. These results indicate that NO exerts multiple effects at the very first stage of synaptic integration in local circuits.
  • H Aonuma, M Iwasaki, K Niwa
    SICE 2004 ANNUAL CONFERENCE, VOLS 1-3 2477 - 2482 2004年 [査読有り][通常論文]
     
    Neuronal mechanism of the formation of cricket hierarchy can be a model system to investigate how animals evoke adaptive behavior, because crickets change behavior after fighting experience. This study demonstrates that NO signaling regulates adaptive behavior that is dependent on memory in insects. The results indicate that NO signaling must be an important factor of switching mechanism in the central nervous systems to evoke adaptive behaviors.
  • H Aonuma
    ZOOLOGICAL SCIENCE 19 9 969 - 979 2002年09月 [査読有り][通常論文]
     
    Nitric oxide (NO) acts as a signalling molecule by activating soluble guanylate cyclase and causing accumulation of the second messenger cyclic guanosine 3',5'-monophosphate (cGMP) in target cells. In order to detect the presence of NO-cGMP signalling pathway in the crayfish abdominal nervous system, accumulation of NO-induced cGMP was investigated by anti-cGMP immunochemistry. Some preparations were incubated in a high-K+ saline containing an inhibitor of cGMP-degrading phosphodiesterase, 3-isobutyl-1-methyxanthine (IBMX), to activate NO generating neurones, which could release NO in the ganglion, and then immunohistochemistry using an anti-cGMP antibody was performed. The other preparations were incubated in NO donor, sodium nitroprusside (SNIP) saline containing IBMX before anti-cGMP immunohistochemistry was performed. The distribution of cGMP-like immunoreactive neurones in high-K+ treated preparations was similar to that of cGMP-like immunoreactive neurones in NO donor treated preparations. About 70-80 cell bodies and many neuronal branches in the neuropilar area of the ganglion were stained, although no neurones showed immunoreactivity unless preparations were activated by either high-K+ or the NO donor. Some of them were identical neurones, and they were intersegmental ascending interneurones and motor neurones. Sensory afferents that innervates hind gut showed strong cGMP-like immunoreactivity, although no mechanosensory afferents showed any immunoreactivity. These results strongly suggest the presence of an NO-cGMP signalling pathway that regulates neuronal events in the abdominal nervous system of the crayfish.
  • H Aonuma, PL Newland
    JOURNAL OF NEUROBIOLOGY 52 2 144 - 155 2002年08月 [査読有り][通常論文]
     
    We have analyzed the action of nitric oxide on the synaptic inputs of spiking local interneurons that form part of the local circuits in the terminal abdominal ganglion of the crayfish, Pacifastacus leniusculus. Increasing the availability of NO in the ganglion by bath applying the NO donor SNAP, or the substrate for its synthesis, L-arginine, caused a depression of synaptic inputs onto the interneurons evoked by electrically stimulating mechanosensory neurons in nerve 2 of the terminal ganglion. Conversely, reducing the availability of NO by bath application of an NO scavenger, PTIO, and an inhibitor of nitric oxide synthase, L-NAME, increased the amplitude of the evoked potentials. These results suggest that elevated NO concentration causes a depression of the synaptic inputs to spiking local interneurons. To determine whether these effects could be mediated through an NO/cGMP signaling pathway we bath applied a membrane permeable analogue of cGMP, 8-br-cGMP, which decreased the amplitude of the inputs to the interneurons. Bath application of an inhibitor of soluble guanlylyl cyclase, ODQ, produced an increase in the amplitude of the synaptic inputs. Our results suggest that NO causes a depression of synaptic inputs to spiking local interneurons probably by acting through an NO/cGMP signaling pathway. Moreover, application of NO scavengers modulates the inputs to these interneurons, suggesting that NO is continuously providing a powerful and dynamic means of modulating the outputs of local circuits. (C) 2002 Wiley Periodicals. Inc.
  • S Fujie, H Aonuma, Ito, I, A Gelperin, E Ito
    ZOOLOGICAL SCIENCE 19 1 15 - 26 2002年01月 [査読有り][通常論文]
     
    To examine the distribution of nitric oxide (NO)-generative cells and NO-responsive cells in the tentacles and procerebral lobes (olfactory processing center) of terrestrial slugs, we applied NADPH diaphorase (NADPH-d) histochemistry and NO-induced cyclic GMP (cGMP)-like immunohistochemistry. We found that NADPH-d reactive cells/fibers and cGMP-like immunoreactive cells/fibers were different, but they were localized adjacent to each other, in both the tentacles and the procerebral lobes. Then, we measured the concentration of NO that was generated around the procerebral lobes using an NO sensitive electrode, when the olfactory nerve was electrically stimulated as a replacement for an odorant stimulus. Stimulation of the olfactory nerve evoked an increase in NO concentration at nanomolar levels, suggesting that binding of nanomolar concentrations of NO to the prosthetic heme group activates soluble guanylyl cyclase. Taken together with previously reported physiological data, our results, therefore, showed that the NO/cGMP pathways are involved in slug olfactory processing.
  • H Schuppe, H Aonuma, PL Newland
    CELL AND TISSUE RESEARCH 305 1 135 - 146 2001年07月 [査読有り][通常論文]
     
    Previous neuropharmacological studies have described the presence of a nitric oxide-cGMP signalling pathway in the crayfish abdominal nervous system. In this study we have analysed the distribution of putative nitric oxide synthase (NOS)-containing ascending interneurones in the crayfish terminal abdominal ganglion using NADPH-diaphorase (NADPHd) histochemistry. Ascending intersegmental interneurones were stained intra-cellularly using the fluorescent dye Lucifer yellow and the ganglia containing the stained interneurones subsequently processed for NADPHd activity. Fluorescence persisted throughout histochemical processing. These double-labelling experiments showed that 12 of 18 identified ascending interneurones were NADPHd positive. Thus many ascending interneurones that process mechanosensory signals in the terminal ganglion may contain NOS, and are themselves likely sources of NO which is known to modulate their synaptic inputs. Three clear relationships emerged from our analysis between the effects of NO on the synaptic inputs of interneurones, their output properties and their staining for NADPH-diaphorase. First were class I interneurones with no local outputs in the terminal ganglion, the NE type interneurones, which had sensory inputs that were enhanced by NO and were NADPHd positive. Second were class I interneurones with local and intersegmental output effects that had sensory inputs that were also enhanced by NO but were NADPHd negative. Third were class 2 interneurones with local and intersegmental outputs that had synaptic inputs that were depressed by the action of NO but were NADPHd positive. These results suggest that NO could selectively enhance specific synaptic connections and sensory processing pathways in local circuits.
  • H Aonuma, PL Newland
    JOURNAL OF EXPERIMENTAL BIOLOGY 204 7 1319 - 1332 2001年04月 [査読有り][通常論文]
     
    Little is known of the action of nitric oxide (NO) at the synaptic level on identified interneurones in local circuits that process mechanosensory signals. Here, we examine the action of NO in the terminal abdominal ganglion of the crayfish Pacifastacus leniusculus, where it has modulatory effects on the synaptic inputs of 17 identified ascending interneurones mediated by electrical stimulation of a sensory nerve. To analyse the role of NO in the processing of sensory signals, we bath-applied the NO donor SNAP, the NO scavenger PTIO, the nitric oxide synthase (NOS) inhibitor L-NAME, the NOS substrate L-arginine, a cyclic GMP (cGMP) analogue, 8-Br-cGMP, and the soluble guanylate cyclase (sGC) inhibitor ODQ. The effects of these chemicals on the synaptic inputs of the interneurones could be divided into two distinct classes. The NO donor SNAP enhanced the inputs to one class of interneurone (class 1) and depressed those to another (class 2). Neither the inactive isomer NAP nor degassed SNAP had any effect on the inputs to these same classes of interneurone, The NO scavenger PTIO caused the opposite effects to those of the NO donor SNAP, indicating that endogenous NO may have an action in local circuits. Preventing the synthesis of NO using L-NAME had the opposite effect to that of SNAP an each response class of interneurone, Increasing the synthesis of endogenous NO by applying L-arginine Led to effects on both response classes of interneurone similar to those of SNAP, Taken together, these results suggested that NO was the active component in mediating the changes in amplitude of the excitatory postsynaptic potentials. Finally, the effects of 8-Br-cGMP were similar to those of the NO donor, indicating the possible involvement of a NO-sensitive guanylate cyclase, This was confirmed by preventing the synthesis of cGMP by sGC using ODQ, which caused the opposite effects to those of 8-Br-cGMP on the two response classes of interneurone, The results indicate that a NO-cGMP signal transduction pathway, in which NO regulates transmitter release from mechanosensory afferents onto intersegmental ascending interneurones, is probably present in the local circuits of the crayfish.
  • H Schuppe, H Aonuma, PL Newland
    CELL AND TISSUE RESEARCH 303 2 289 - 299 2001年02月 [査読有り][通常論文]
     
    Nitric oxide (NO) has an important modulatory role on the processing of sensory signals in vertebrates and invertebrates. In this investigation we studied the potential sources of NO in the terminal abdominal ganglion of the crayfish, Pacifastacus leniusculus, using NADPH-diaphorase (NADPHd) histochemistry, with NADPHd acting as a marker for NO synthase (NOS). In the terminal ganglion a mean of 27 strongly labelled NADPHd-positive cell bodies were found, and of these 8% occurred in three regions located in antero-lateral, central and posterior parts of the ganglion. Ventral and antero-ventral commissures as well as specific dorsal and ventral areas of the dendritic neuropil showed positive staining. Intense labelling was seen in the ventro-medial tract, and in the connective between the terminal ganglion and the 5th abdominal ganglion. In addition, some motor neurones and neurones with branches in the sensory commissures were NADPHd positive. Our finding that NADPHd-positive cells occur in consistent patterns in the terminal abdominal ganglion implies that NO may have a role in mechanosensory processing in the crayfish.
  • Schuppe H, Aonuma H, Newland P.L
    Cell and Tissue Research 303 3 451  2001年 [査読有り][通常論文]
  • PL Newland, H Aonuma, T Nagayama
    ZOOLOGICAL SCIENCE 17 9 1185 - 1195 2000年12月 [査読無し][通常論文]
     
    A single proprioceptor in the tailfan of the crayfish, Procambarus clarkii (Girard), innervated by only twelve sensory neurones encodes the position and the direction and velocity of movement of the exopodite relative to the endopodite. Most of the sensory neurones project to, and terminate in, the terminal abdominal ganglion where they form a map in which projection position is based on the velocity threshold of the sensory neurone. The sensory signals from this small proprioceptor have significant effects on the neuronal circuits mediating escape swimming and activate the lateral giant interneurone directly through monosynaptic connections and indirectly via a disynaptic pathway involving a number of interposed intersegmental interneurones. The lateral giant interneurones are activated through electrical synapses whereas the ascending interneurones in the disynaptic pathway are excited through both electrical and chemical synapses. The proprioceptive signals are also responsible for evoking widespread presynaptic inhibition of exteroceptive afferents that reduces the efficacy of their outputs. This pathway therefore reduces afference caused by water movement as a result of an animals own escape movements. Movements of the chordotonal organ also lead to a delayed input to giant motor neurone that is timed to occur during flexion movements of the abdomen. Thus not only do the proprioceptive signals activate the escape pathway leading to a tail-flip, but they also protect it from unwanted sensory input, and may also prevent depression of its neuromuscular synapses.
  • H Aonuma, T Nagayama, M Takahata
    JOURNAL OF EXPERIMENTAL BIOLOGY 203 23 3595 - 3602 2000年12月 [査読有り][通常論文]
     
    A characteristic physiological property of the neuromuscular junction between giant motor neurones (MoGs) and fast flexor muscles in crayfish is synaptic depression, in which repetitive electrical stimulation of the MoG results in a progressive decrease in excitatory junction potential (EJP) amplitude in flexor muscle fibres, Previous studies have demonstrated that L-arginine (L-Arg) modulates neuromuscular transmission. Since L-Arg is a precursor of nitric oxide (NO), we examined the possibility that NO may be involved in modulating neuromuscular transmission from MoGs to abdominal fast flexor muscles, The effect of a NO-generating compound, NOC7, was similar to that of L-Arg, reversibly decreasing the EJP amplitude mediated by the MoG, While NOC7 reduced the amplitude of the EJP, it induced no significant change in synaptic depression. In contrast, a scavenger of free radical NO, carboxy-PTIO, and an inhibitor of nitric oxide synthase, L-NAME, reversibly increased the EJP amplitude mediated by MoGs, Synaptic depression mediated by repetitive stimulation of MoGs at 1 Hz was partially blocked by bath application of L-NAME. Bath application of a NO scavenger, a NOS inhibitor and NO-generating compounds had no significant effects on the depolarisation of the muscle fibres evoked by local application of L-glutamate, The opposing effects on EJP amplitude of NOC7 and of carboxy-PTIO and L-NAME suggest that endogenous NO presynaptically modulates neuromuscular transmission and that it could play a prominent role at nerve terminals in eliciting MoG-mediated synaptic depression in the crayfish Procambarus clarkii.
  • H Aonuma, PL Newland, T Nagayama
    JOURNAL OF EXPERIMENTAL BIOLOGY 202 21 2975 - 2984 1999年11月 [査読有り][通常論文]
     
    Intersegmental interneurones are crucial for the appropriate coordination of the activity of local circuits located in different body segments. We have analysed the synaptic inputs to ascending intersegmental interneurones from a proprioceptor in the tailfan of the crayfish. Twenty identified interneurones responded during stimulation of the exopodite-endopodite chordotonal organ. Of these 20 interneurones, three were excited phase-tonically, nine were excited phasically and eight were inhibited.,All received convergent exteroceptive inputs from water-motion- or touch-sensitive hairs on the uropods, The effects of simultaneous exteroceptive and proprioceptive stimulation depended upon the identity of an interneurone. For interneurones that were inhibited by proprioceptive stimulation, suprathreshold exteroceptive responses were reduced to a subthreshold level by simultaneous proprioceptive stimulation. In contrast, for interneurones that were excited by proprioceptive stimulation, the simultaneous application of subthreshold proprioceptive and exteroceptive stimulation elicited action potentials. Two of the interneurones that receive proprioceptive input (NE-1 and RC-8) are known to be presynaptic to giant interneurones that mediate and coordinate the tailhip, Many of the other interneurones that receive proprioceptive inputs in the tailfan are known to excite abdominal extensor motor neurones. Thus, proprioceptive input to these intersegmental interneurones could serve two roles: first, to extend the abdomen during postural movements or prior to escape and, second, to drive the tailhip escape response.
  • H Aonuma, T Nagayama
    JOURNAL OF COMPARATIVE NEUROLOGY 410 4 677 - 688 1999年08月 [査読有り][通常論文]
     
    In the first step toward identifying the neurotransmitter released from spiking interneurons of both local and intersegmental groups in the crayfish terminal abdominal ganglion, the authors examined whether spiking local interneurons and ascending intersegmental interneurons contain the transmitter gamma-aminobutyric acid (GABA). In this paper, 17 identified ascending interneurons and three spiking local interneurons were stained by intracellular injection of Lucifer yellow and subsequently treated for immunocytochemical staining against GABA. Double-labeling experiments revealed that six identified ascending interneurons are GABAergic, but no spiking local interneurons show GABA-like immunoreactivity Four ascending interneurons with GABA-like immunoreactivity (reciprocal closing ascending neuron 5 [RC-5], reciprocal opening ascending neuron 6 [RO-6], variable-effect ascending interneuron 1 [VE-1], and no-effect ascending: interneuron 4[NE-4]) had cell bodies that formed a duster on the ventral surface of the rostral edge of the ganglion, whereas two GABAergic interneurons (coinhibiting ascending interneuron 2 [CI-2] and NE-2) had cell bodies in a caudal region around the cell body of the seventh flexor inhibitor (FI) motor neuron. Another four rostral interneurons (RC-2, RC-3, RC-4, and NE-3) and seven caudal interneurons (CI-3, RC-7, RO-1, RO-2, RO-3, RO-4, and NE-1) had no GABA-like immunoreactivity. Because VE-1 is known to make direct inhibitory connections with other ascending interneurons, whereas RC-3 and RO-1 are known to make direct excitatory connections, the immunocytochemical results from this study are consistent with previous physiological studies. Although many spiking local interneurons (including spiking local interneuron 1 of the anterior group [sp-ant1]) made direct inhibitory connections with nonspiking local interneurons, three spiking local interneurons (sp-ant1, spiking local interneuron 6 of the medial group [sp-med6], and spiking interneuron 5 of the posterior group [sp-post]) do not show GABA-like immunoreactivity. These results suggest that the inhibitory transmitter released from spiking local interneurons is not GABA but that another substance mediates the inhibitory action of these interneurons. (C) 1999 Wiley-Liss, Inc.
  • H Aonuma, T Nagao, T Nagayama, M Takahata
    JOURNAL OF EXPERIMENTAL ZOOLOGY 283 6 531 - 540 1999年05月 [査読有り][通常論文]
     
    The modulatory effects of amino acids on neuromuscular transmission from an identified giant motor neuron (MoG) and abdominal fast flexor muscles of the crayfish were examined using electrophysiological and pharmacological techniques. The distribution of amino acids in the cell body and axon of a single MoG was revealed using high-performance liquid chromatography with electrochemical detection. Eight different amino acids-aspartate, glutamate, glutamine, arginine, glycine, taurine, alanaine and gamma-aminobutyric acid-were simultaneously detected in either the cell body or the axon of MoG. Aspartate, glutamate, alanine and arginine were present at relatively high concentration. Local pressure ejection of glutamate caused a depolarization of the abdominal fast flexor muscle fibers. On the other hand, aspartate, alanine and arginine had no clear effects on the same muscle fibers. Aspartate and arginine, however, had modulatory effects on neuromuscular transmission. Alanine had no significant effect on the neuromuscular transmission. Aspartate at a concentration of 200 mu M decreased the amplitude of EJPs in the fast flexor muscle mediated by stimulation of both the MoG and non-giant fast flexor motor neurons. Arginine at a concentration of 200 mu M reduced the EJP amplitude of the muscle fibers in response to MoG stimulation but enhanced the EJP amplitude of the same muscle fibers by stimulation of non-giant fast flexor motor neurons. Although rather high concentration (1 mM) were required, aspartate increased and arginine decreased the depolarization of the muscle fibers induced by local ejection of glutamate. The opposite effect of arginine on the fast flexor muscles in response to the stimulation of different motor neurons suggested its modulatory role in the different effects of these motor neurons (depression and facilitation) in the fast flexor muscles. J. Exp. Zool. 283:531-540, 1999. (C) 1999 Wiley-Liss, Inc.
  • T Nagayama, H Namba, H Aonuma
    JOURNAL OF COMPARATIVE NEUROLOGY 389 1 139 - 148 1997年12月 [査読有り][通常論文]
     
    The inhibitory neurotransmitter of premotor nonspiking local interneurones in the crayfish terminal abdominal ganglion was investigated physiologically and immunocytochemically. Depolarization of a nonspiking interneurone evoked a hyperpolarization in a uropod motor neurone. The amplitude of hyperpolarization in the motor neurone was gradually decreased under low-calcium/high-magnesium saline. Local pressure injection of gamma-aminobutyric acid (GABA) into the neuropil caused a similar hyperpolarization of the motor neurone. These physiological studies suggested a GABAergic inhibitory interaction between nonspiking interneurones and the motor neurones. Premotor nonspiking interneurones are classified into two subgroups of posterolateral (PL) and anterolateral (AL) interneurones, and AL interneurones are further divided into three subtypes. A combination of intracellular staining from nonspiking local interneurones with Lucifer yellow and immunocytochemical staining with an antiserum directed against GABA revealed that all the PL interneurones sampled in this study showed GABA-like immunoreactivity. A population of cell bodies (n = 6-11) with a small diameter (15-30 mu m) packed together forming a cluster showed GABA-like immunoreactivity, and the cell bodies of most PL interneurones were found in this cluster. To compare the number and the pattern of main branches of PL interneurones, cells were classified into three identifiable sets of interneurones, called PL-1, PL-2, and PL-3. By contrast, about one-half of AL interneurones, especially the third subtype of AL interneurones, which have cell bodies located ventrolaterally in the ganglion, did not show GABA-like immunoreactivity. Furthermore, the position of cell bodies of GABA-immunoreactive AL interneurones was scattered compared to that of PL interneurones. (C) 1997 Wiley-Liss, Inc.
  • PL Newland, H Aonuma, T Nagayama
    JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY 181 2 103 - 109 1997年08月 [査読有り][通常論文]
     
    Giant interneurones mediate a characteristic 'tail flip' escape response of the crayfish, Procambarus clarkii, which move it rapidly away from the source of stimulation. We have analysed the synaptic connections of proprioceptive sensory neurones with one type of giant interneurone, the lateral giant. Spikes in sensory neurones innervating an exopodite-endopodite chordotonal organ in the tailfan, which monitors the position and movements of the exopodite, are followed at a short and constant latency by excitatory postsynaptic potentials in a lateral giant interneurone (LG) recorded ill the terminal abdominal ganglion. These potentials are unaffected by manipulation of the membrane potential of LG, by bath application of saline with a low calcium concentration, or by one containing the nicotinic antagonist, curare. The potentials evoked in LG by chordotonal organ stimulation are thus thought to be monosynaptic and electrically mediated. This is the first demonstration that LG receives input from sensory receptors other than exteroceptors in the terminal abdominal ganglion.
  • T Nagayama, H Aonuma, PL Newland
    JOURNAL OF NEUROPHYSIOLOGY 77 5 2826 - 2830 1997年05月 [査読有り][通常論文]
     
    Synaptic transmission between proprioceptive afferents from a chordotonal organ in the tailfan of the crayfish and an identified ascending interneuron, interneuron A, in the terminal abdominal ganglion was analyzed. Interneuron A is part of a disynaptic pathway from primary afferent neurons to the lateral giant interneuron involved in producing the characteristic ballistic escape behavior of crayfish. Interneuron A received short and long latency excitatory postsynaptic potentials (EPSPs) from chordotonal afferents. Short latency EPSPs occurred with Little central synaptic delay were unchanged by hyperpolarizing current injection of -2 nA, and remained at a constant amplitude when the nervous system was bathed in saline with a low calcium concentration or saline containing the nicotinic antagonist curare. These EPSPs are thus thought to be mediated by electrical transmission. Longer latency potentials were increased in amplitude by hyperpolarizing current injection, reduced in amplitude when the nervous system was bathed in low-calcium saline, and also reduced by bath application of saline containing curare. These potentials are thus thought to be mediated by chemical transmission. The functional significance of the dual modes of transmission at a key synapse in the escape circuitry is discussed.
  • T Nagayama, H Aonuma, H Miyata
    JOURNAL OF EXPERIMENTAL BIOLOGY 199 11 2447 - 2450 1996年11月 [査読有り][通常論文]
     
    Using an antiserum directed against gamma-aminobutyric acid (GABA) to label neurones with GABA-like immunoreactivity, approximately 70 central neurones (68+/-9; mean +/- S.E.M., N=9) were labelled in the terminal abdominal ganglion of the crayfish Procambarus clarkii. This mean number of neurones with GABA-like immunoreactivity represents approximately 10% of the total number of neurones in the terminal ganglion. A combination of intracellular staining using Lucifer Yellow and immunocytochemical staining revealed that an identified nonspiking local interneurone (the local directionally selective interneurone, LDS) showed GABA-like immunoreactivity.
  • PL Newland, H Aonuma, M Sato, T Nagayama
    JOURNAL OF NEUROPHYSIOLOGY 76 2 1047 - 1058 1996年08月 [査読有り][通常論文]
     
    1. Exteroceptive hairs that are sensitive to water displacement and touch are distributed over the surface of the tailfan of crayfish. We show that the sensory neurons innervating these hairs receive a primary afferent depolarization (PAD) from sensory neurons innervating a proprioceptor that monitors movements of the endopodite and protopodite of the tailfan. This PAD occurs only during high-velocity movements of the exopodite, which are similar to those that occur during swimming. The effects that the proprioceptor mediate are widespread, so that afferents in four sensory nerve roots of the terminal abdominal ganglion, innervating hairs on the protopodite, exopodite, endopodite, and telson, receive a PAD. The PAD is unlikely to be mediated through monosynaptic pathways because there is no anatomic overlap between the central projections of chordotonal afferents and many of the exteroceptive afferents. The depolarization is associated with a conductance increase and can be increased by the injection of hyperpolarizing current or reversed (similar to 10 mV above resting potential) by injection of depolarizing current. The properties of the presynaptic input are, therefore, consistent with being mediated through chemical synapses. This is supported by the observation in the electron microscope that the exteroceptive afferents receive chemical input synapses. The depolarization is mimicked by gamma-aminobutyric acid and reduced by bath application of picrotoxin or bicuculline, suggesting that it is a depolarizing inhibitory postsynaptic potential. The PAD reduces the amplitude of exteroceptive afferent spikes, an action that is thus likely to reduce transmitter release and the efficacy of synaptic transmission.
  • H Aonuma, T Nagayama, M Takahata
    JOURNAL OF EXPERIMENTAL ZOOLOGY 275 6 406 - 412 1996年08月 [査読有り][通常論文]
     
    Some central neurons in the crayfish have autofluorescent cell bodies. The yellow fluorescence of cell bodies was induced in the whole-mount preparation by using blue-violet light from a high-pressure mercury lamp. From the first to the fifth abdominal ganglion, a pair of cell bodies fluoresced in each segmental ganglion. In the sixth abdominal ganglion, two pairs of fluorescent cell bodies were detected. A pair of cell bodies also fluoresced in the posterior (fourth and fifth) thoracic ganglia, while the probability of finding fluorescent cell bodies in the anterior (first to third) thoracic ganglia was very low. Intracellular staining revealed 1) that autofluorescent cells were motor giants (MoGs) and 2) their homologue in the abdominal and thoracic ganglia. This characteristic of autofluorescence provides the cue of ready identification of particular crayfish central neurons, which are one of the most essential neural components for intrinsic escape behavior. (C) 1996 Wiley-Liss, Inc.
  • T NAGAYAMA, H NAMBA, H AONUMA
    HISTOLOGY AND HISTOPATHOLOGY 9 4 791 - 805 1994年10月 [査読有り][通常論文]
     
    As the subject of neuroethological studies, arthropods offer several advantages to elucidate the neural processes that generate and control behavioural acts. The relative simplicity of their neural organization and their identifiable neurones are particularly advantageous. The crayfish, Procambarus clarkii, uropods are paired appendages of the last abdominal segment that show bilateral closing movement in response to mechanical stimulation. This movement, the avoidance ''dart'' response, is mediated by a cascade and parallel organization of local circuit neurones in the terminal abdominal ganglion. Thirty intersegmental ascending interneurones and 20 spiking local interneurones have been identified both morphologically and physiologically. They receive exteroceptive inputs directly from the sensory afferents. Two complementary groups of unilateral nonspiking local interneurones have also been functionally identified. They exert fine control over the wide range of activity of motor neurones in a graded and sustained manner. These nonspiking interneurones form opposing and parallel pathways that are essential in modulating the pattern of movement of the uropods.
  • H AONUMA, T NAGAYAMA, M HISADA
    ZOOLOGICAL SCIENCE 11 2 191 - 202 1994年04月 [査読有り][通常論文]
     
    The output effects of 16 identified ascending interneurons originating in the terminal abdominal ganglion were examined with intracellular recording and stimulating techniques in isolated nerve cord composed of 6 abdominal ganglia (from the 1st to 6th ganglion). The activity of the uropod closer and opener motor neurons was recorded extracellularly with pin electrodes from the terminal abdominal (A6) ganglion and that of the abdominal extensor and flexor motor neurons was recorded from the 1st to 5th abdominal (A1-A.5) ganglion. This technique allowed us to monitor the activity of the motor neurons innervating different muscles of more than 10 simultaneously in the same preparation. Majority of ascending interneurons had output effects upon not only the uropod motor neurons but also the abdominal postural motor neurons. The premotor effects of the ascending interneurons were the same in all of abdominal ganglia (A1-A5). Some ascending interneurons also affected the abdominal postural motor neurons on both sides of each ganglion with a similar fashion. Neurobiotin staining revealed that the ascending axons spreaded their branches in each abdominal ganglion. Their branches were extended within the side ipsilateral to their axons. The possible function of ascending interneurons as multi-functional units in the sensory-motor system of crayfish was discussed. Since they received sensory inputs from the tailfan and affected the activity of both uropod and abdominal postural motor neurons simultaneously, they would coordinate the behavioural sequence controlling both the uropod motor system and abdominal postural system.

書籍

  • Cricket as a model organism for the 21st century; Development, Regeneration, and Behavior
    青沼 仁志 (担当:分担執筆範囲:Fighting behavior: Understanding the mechanisms of group-size-dependent aggression, Synthetic approaches for observing and measuring cricket behaviors)
    Springer 2017年
  • 行動生物学辞典
    青沼 仁志 (担当:分担執筆)
    東京化学同人 2013年
  • 太田 順, 青沼 仁志 (担当:共編者(共編著者))
    オーム社 2010年03月 (ISBN: 4274502791) 284
  • 身近な動物を使った学実験4 ミツバチ コオロギ スズメガ
    三共出版 2009年
  • 動物は何を考えているのか?:学習と記憶の比較生物学
    共立出版 2009年
  • 昆虫ミメティクス-昆虫の設計に学ぶ
    エヌ・ティー・エス企画 2008年
  • COE成果本 基礎編 IV-3個体のバイオサイエンス
    北大出版会 2006年
  • The Crustacean Nervous System (ed. Wiese K.)
    Springer-Verlag 2001年
  • 太田 順, 青沼 仁志 
    オーム社 (ISBN: 9784274502804)

その他活動・業績

  • Keisuke Naniwa, Hitoshi Aonuma 2020年11月03日 
    AbstractThe cricket is one of the model animals used to investigate the neuronal mechanisms underlying adaptive locomotion. An intact cricket walks with a tripod gait, similar to other insects. The motor control center of the leg movements is located in the thoracic ganglia. In this study, we investigated the walking gait patterns of crickets whose ventral nerve cords were surgically cut to gain an understanding of how the descending signals from the head ganglia and ascending signals from the abdominal nervous system into the thoracic ganglia mediate the initiation and coordination of the walking gait pattern. Crickets whose paired connectives between the brain and subesophageal ganglion (SEG) were cut exhibited a tripod gait pattern. However, when one side of the connectives between the brain and SEG was cut, the crickets continued to turn in the opposite direction to the connective cut. Crickets whose paired connectives between the SEG and prothoracic ganglion were cut did not walk, whereas the crickets exhibited an ordinal tripod gait pattern when one side of the connectives was intact. Crickets whose paired connectives between the metathoracic ganglion and abdominal ganglia were cut initiated walking, although the gait was not a coordinated tripod pattern, whereas the crickets exhibited a tripod gait when one side of the connectives was intact. These results suggest that the brain plays an inhibitory role in initiating leg movements, and that both the descending signals from the head ganglia and the ascending signals from the abdominal nervous system are both important in initiating and coordinating insect walking gait patterns.
  • Daiki Wakita, Keisuke Naniwa, Hitoshi Aonuma 2019年05月 [査読有り][通常論文]
  • Daiki Wakita, Katsushi Kagaya, Hitoshi Aonuma 2019年04月 [査読有り][通常論文]
  • 大須賀 公一, 青沼 仁志, 岡田 美智男, 石黒 章夫, 大須賀 公一, 石川 将人 システム/制御/情報 63 (6) 246 -253 2019年 [査読無し][通常論文]
  • Keisuke Naniwa, Yasuhiro Sugimoto, Koichi Osuka, Hitoshi Aonuma 2018年07月 [査読有り][通常論文]
  • 浪花啓右, 青沼仁志, 青沼仁志, 杉本靖博, 大須賀公一, 大須賀公一 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2018 ROMBUNNO.1P1‐D13 2018年06月01日 [査読無し][通常論文]
  • 和田啓雅, 杉本靖博, 青沼仁志, 浪花啓右, 大須賀公一 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2018 ROMBUNNO.2P1‐D12 2018年06月01日 [査読無し][通常論文]
  • 早瀬友美乃, 早瀬友美乃, 中西秀, 坂上貴洋, 青沼仁志, 高原聡, 金子俊一 日本物理学会講演概要集(CD-ROM) 73 (1) ROMBUNNO.24pK604‐13 2018年03月23日 [査読無し][通常論文]
  • 浪花啓右, 杉本靖博, 青沼仁志, 大須賀公一 自律分散システム・シンポジウム(CD-ROM) 30th ROMBUNNO.1A2‐3 2018年01月28日 [査読無し][通常論文]
  • 青沼仁志, 青沼仁志 自律分散システム・シンポジウム(CD-ROM) 30th ROMBUNNO.1A2‐2 2018年01月28日 [査読無し][通常論文]
  • 大脇大, 杉本靖博, 石黒章夫, 石黒章夫, 青沼仁志, 青沼仁志 自律分散システム・シンポジウム(CD-ROM) 30th ROMBUNNO.1A2‐4 2018年01月28日 [査読無し][通常論文]
  • 安井浩太郎, 菊池和気, 加納剛史, 黒田茂, 青沼仁志, 早瀬友美乃, 小林亮, 石黒章夫 自律分散システム・シンポジウム(CD-ROM) 30th ROMBUNNO.1A3‐3 2018年01月28日 [査読無し][通常論文]
  • 大須賀公一, 大須賀公一, 青沼仁志, 青沼仁志 自律分散システム・シンポジウム(CD-ROM) 30th ROMBUNNO.1A2‐1 2018年01月28日 [査読無し][通常論文]
  • Masahiro Shimizu, Daisuke Ishii, Hitoshi Aonuma, Koh Hosoda 2017 IEEE International Conference on Cyborg and Bionic Systems, CBS 2017 2018-January 73 -76 2018年01月19日 [査読無し][通常論文]
     
    © 2017 IEEE. This paper shows a frog cyborg Xenopus-noid with webbed feet based on the anatomical structure of feet of X. laevis. Locomotion of an animal is generated through interactions among the nervous system, the musculo-skeletal system and the environment. We particularly focus on bio-machine hybrid systems in order to understand dynamics of these interactions. Under this circumstance, we propose a frog cyborg driven by dissected muscles from frog legs, and webbed feet generate efficient hydrodynamic propulsion for good frog-like swimming.
  • 早瀬友美乃, 早瀬友美乃, 中西秀, 坂上貴洋, 青沼仁志, 高原聡, 松田朝陽, 金子俊一 日本物理学会講演概要集(CD-ROM) 72 (2) ROMBUNNO.21aK10‐9 2017年09月25日 [査読無し][通常論文]
  • 杉本靖博, 浪花啓右, 青沼仁志, 大須賀公一 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2017 ROMBUNNO.1A1‐E10 2017年05月09日 [査読無し][通常論文]
  • 小野達也, 金内大地, 加納剛史, 青沼仁志, 青沼仁志, 石黒章夫, 石黒章夫 自律分散システム・シンポジウム(CD-ROM) 29th ROMBUNNO.1A1‐2 2017年01月30日 [査読無し][通常論文]
  • 安井浩太郎, 菊池和気, 加納剛史, 早瀬友美乃, 黒田茂, 青沼仁志, 青沼仁志, 小林亮, 小林亮, 石黒章夫, 石黒章夫 自律分散システム・シンポジウム(CD-ROM) 29th ROMBUNNO.1A3‐1 2017年01月30日 [査読無し][通常論文]
  • Hitoshi Aonuma The Cricket as a Model Organism: Development, Regeneration, and Behavior 313 -325 2017年01月01日 [査読有り][通常論文]
     
    When we investigate animal behavior, it is necessary to quantify and to qualify the sequence of observed behaviors. It is also important to compare the behavior with the physiology of the nervous systems in order to understand underlying neuronal mechanisms. Reliable results require experimental repetition that includes consistent controls, because animals do not always respond in the same way to the same external stimuli. Instead, animals alter their behaviors in order to respond to the demands of changing environments. Engineering approaches, in particular robotics, can help us to observe and to provoke animal movements and behaviors. I describe a novel approach that provokes animal movements and behaviors in response to computer simulation and robots. These constructive approaches help us to bridge the gap between behavior and physiology. The performances of the models and robots are discussed, and the accuracies of the models are confirmed by behavior studies with animals. This approach has been named “Synthetic Neuroethology.” This chapter introduces the methods used to observe and measure cricket behaviors. The aim is to understand adaptive behaviors at play in group size-dependent aggressive behavior.
  • 2017 IEEE INTERNATIONAL CONFERENCE ON CYBORG AND BIONIC SYSTEMS (CBS) 73 -76 2017年 [査読有り][通常論文]
  • Hitoshi Aonuma, Koichi Osuka, Kyohsuke Ohkawara 2017 56TH ANNUAL CONFERENCE OF THE SOCIETY OF INSTRUMENT AND CONTROL ENGINEERS OF JAPAN (SICE) 2017-November 15 -18 2017年 [査読有り][通常論文]
     
    The ant Ondontomuchus kuroiwae has a trap jaw that snaps quickly to capture a prey. The ant opens the trap jaw, locks it and closes quickly if it detects a prey using a sensory hair on the jaw. We here analyze 3-dimensional structure of the trap jaw muscles by using a micro volume imaging with an X-ray micro-computed tomography (micro-CT) to elucidate the mechanism of the exoskeletal muscular system to realize ultra-high speed movement of the trap jaw.
  • Aonuma, H. The Cricket as a Model Organism: Development, Regeneration, and Behavior 197 -209 2017年 [査読有り][通常論文]
  • 杉本靖博, 浪花啓右, 青沼仁志, 大須賀公一 横幹連合コンファレンス(CD-ROM) 8th ROMBUNNO.D‐2‐3 2017年 [査読無し][通常論文]
  • 長谷川英祐, 青沼仁志, 寺尾勘太, 小楠なつき, 大久保祐作, 渡邊紗織, 三上俊太, 藤田悠介, 水波誠, 村上優花 日本動物行動学会大会発表要旨集 36th P.185 2017年 [査読無し][通常論文]
  • 杉本 靖博, 浪花 啓右, 青沼 仁志, 大須賀 公一 横幹連合コンファレンス予稿集 2017 (0) D -2-3 2017年 [査読無し][通常論文]
     
    In this research, to investigate behavior and internal state changes during aggressive behaviorof cricket, we are developing an small autonomous mobile robot which has a articial antennae and canintervene in aggressive behavior repeatedly.
  • 杉本 靖博, 浪花 啓右, 青沼 仁志, 大須賀 公一 ロボティクス・メカトロニクス講演会講演概要集 2017 (0) 1A1 -E10 2017年 [査読無し][通常論文]
     
    <p>In this study, towards the neurophysiological mechanism elucidation of the aggressive behavior of crickets, in order to examine the internal state of the aggressive behavior. Therefore, we have developed a small mobile invasive device that can intervene in aggressive behavior of cricket. In this paper, by useing a small mobile invasive device, it is showed that the locomotion of crickets greatly influences the probability of aggressive behavior.</p>
  • 小野達也, 加納剛史, 青沼仁志, 青沼仁志, 松坂義哉, 石黒章夫, 石黒章夫 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2016 ROMBUNNO.1A2‐04b2 2016年06月08日 [査読無し][通常論文]
  • 坂井亮, 清水正宏, 青沼仁志, 細田耕 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2016 ROMBUNNO.1A1‐12a2 2016年06月08日 [査読無し][通常論文]
  • 杉本靖博, 浪花啓右, 青沼仁志, 大須賀公一 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2016 ROMBUNNO.1A2‐05a6 2016年06月08日 [査読無し][通常論文]
  • 青沼仁志, 青沼仁志 自律分散システム・シンポジウム(CD-ROM) 28th ROMBUNNO.2A2‐1 2016年01月21日 [査読無し][通常論文]
  • 坂井 亮, 清水 正宏, 青沼 仁志, 細田 耕 ロボティクス・メカトロニクス講演会講演概要集 2016 (0) 1A1 -12a2 2016年 [査読無し][通常論文]
     
    <p>This study aims to understand relationships between musculoskeletal structure of frogs and swimming performance without control of the nerves systems. Frogs generate great swimming performance by extension of the hind limbs. Extension of knee cannot only generate thrust but also give a load to the Plantaris longus (PL) muscle, which is ankle extensor muscle. We developed an aquatic frog robot, Xenopus-noid, which can extend the knee and is driven by the dissected PL muscle from <i>Xenopus laevis</i>. Xenopus-noid has the knee extension mechanism composed of electrical magnet, a torsion spring positioned in hip joint. An energy of a torsion spring is released by turning off of an electrical magnet. In this study, we confirmed that the knee extension of Xenopus-noid is similar to that of <i>X.laevis</i>. Xenopus-noid is expected to be a useful tool to understand the relationship between knee extension of frogs and swimming performance.</p>
  • 小野 達也, 加納 剛史, 青沼 仁志, 松坂 義哉, 石黒 章夫 ロボティクス・メカトロニクス講演会講演概要集 2016 (0) 1A2 -04b2 2016年 [査読無し][通常論文]
     
    <p>Robots are now required to adapt to changes in the environments and physical damages so that they can work under harsh environments. To meet the requirement, we focused on ophiuroid' s highly adaptive and resilient locomotion. In our previous work, we proposed a decentralized control scheme for the well-balanced coupling between inter- and intra-limb coordination, using local force feedback. However, this scheme could not reproduce an avoidance behavior observed when arms detect reaction forces that impede propulsion. To tackle this problem, in this study, we redesigned a decentralized control scheme based on " TEGOTAE ", a Japanese concept describing how well a perceived reaction matches an expectation.</p>
  • 杉本 靖博, 浪花 啓右, 青沼 仁志, 大須賀 公一 ロボティクス・メカトロニクス講演会講演概要集 2016 (0) 1A2 -05a6 2016年 [査読無し][通常論文]
     
    <p>In this study, towards the neurophysiological mechanism elucidation of the aggressive behavior of crickets, in order to examine the internal state of the aggressive behavior. Therefore, we have developed a small mobile robot that can repeatedly induce the aggressive behavior of cricket. In this paper, the small mobile robot ware able to induce the following activities and the antenna fencing which is a part of the aggressive behavior to robots.</p>
  • 渡邊崇之, 青沼仁志 日本進化学会大会プログラム・講演要旨集(Web) 17th ROMBUNNO.P‐18 (WEB ONLY) 2015年08月20日 [査読無し][通常論文]
  • 小野達也, 加納剛史, 青沼仁志, 青沼仁志, 松坂義哉, 石黒章夫, 石黒章夫 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2015 ROMBUNNO.2A2‐S02 2015年05月16日 [査読無し][通常論文]
  • 佐藤英毅, 小野達也, 加納剛史, 青沼仁志, 松坂義哉, 石黒章夫 自律分散システム・シンポジウム(CD-ROM) 27th ROMBUNNO.1C2-3 2015年01月22日 [査読無し][通常論文]
  • Sakai, R., Shimizu, M., Aonuma, H., Hosoda, K. Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) 9222 97 -100 2015年 [査読有り][通常論文]
  • 小野 達也, 加納 剛史, 青沼 仁志, 松坂 義哉, 石黒 章夫 ロボティクス・メカトロニクス講演会講演概要集 2015 (0) _2A2 -S02_1-_2A2-S02_4 2015年 [査読無し][通常論文]
     
    Although numerous robots have been developed inspired by animal locomotion, they could not reproduce adaptive and resilient behavior of real animals. To tackle this problem, we adopted ophiuroids as our model. In our previous work, we focused on the locomotion of an ophiuroid whose arms were shortened or removed to investigate the inter-arm coordination mechanism, and proposed a decentralized control scheme on the basis of "TEGOTAE", a Japanese word that means reaction after the generation of some action. In this study, we proposed a decentralized control scheme that enables well-balanced coupling between inter- and intra-limb coordination by extending our previous control scheme. The validity of the proposed control scheme was confirmed via simulation.
  • 小林 充, 片岡 崇, 青沼 仁志, 柴田 洋一 農業食料工学会誌 77 (3) 179 -185 2015年 [査読無し][通常論文]
     
    <p>鱗翅目害虫のオス成虫はメス成虫の分泌する性フェロモンを触角で感知すると,触角内部に数mVの生体電位(以下,触角電位)が生じる。本研究では,害虫発生予察のために,ヨトウガを対象とし,性フェロモンに対する触角のセンシング機能を検討した。触角はヨトウガの頭部から切り離し,両端から脳波測定用ペーストを介して,計測器へ接続し,触角電位を計測した。この結果,切除した触角の寿命は最長200分程度であること,性フェロモン刺激は5秒以上の間隔を空けることが望ましいことがわかった。そして,メス性フェロモンの主成分である(<i>Z</i>) -11-ヘキサデセニルアセタート濃度に応じて触角電位の電位差は大きくなり,この変化を近似曲線で表すことができた。これより,オス成虫の触角電位応答を計測することで,メス成虫の発生動向の予察の一助になると考えられる。</p>
  • 加納剛史, 佐藤英毅, 小野達也, 青沼仁志, 松坂義哉, 石黒章夫 計測自動制御学会システム・情報部門学術講演会講演論文集(CD-ROM) 2014 ROMBUNNO.GS3-11(O1-3) 2014年11月21日 [査読無し][通常論文]
  • 佐藤英毅, 小野達也, 加納剛史, 青沼仁志, 松坂義哉, 石黒章夫 日本ロボット学会学術講演会予稿集(CD-ROM) 32nd ROMBUNNO.1O2-03 2014年09月04日 [査読無し][通常論文]
  • 坂井亮, 清水正宏, 青沼仁志, 細田耕 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2014 ROMBUNNO.2A1‐J06 2014年05月24日 [査読無し][通常論文]
  • 佐藤英毅, 加納剛史, 青沼仁志, 松坂義哉, 石黒章夫 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2014 ROMBUNNO.1A1‐V05 2014年05月24日 [査読無し][通常論文]
  • K. Kawabata, H. Aonuma, S. Takahashi, K. Hosoda, J. Xue Journal of Signal Processing 2014年05月 [査読有り][通常論文]
  • 行動発現解析に向けたクロコオロギの行動変容計測手法
    奥田泰丈, 高橋悟, 川端邦明, 青沼仁志, 岩田健司, 佐藤雄隆 動的画像処理実利用化ワークショップDIA2014講演論文集 2014年03月 [査読無し][通常論文]
  • 杉本靖博, 青沼仁志, 川端邦明, 大須賀公一 自律分散システム・シンポジウム(CD-ROM) 26th ROMBUNNO.1C3-2 2014年01月23日 [査読無し][通常論文]
  • 加納剛史, 大須賀公一, 小林亮, 小林亮, 青沼仁志, 石川将人, 杉本靖博, 大脇大, 石黒章夫, 石黒章夫 自律分散システム・シンポジウム(CD-ROM) 26th ROMBUNNO.2B2-3 2014年01月23日 [査読無し][通常論文]
  • 川端邦明, 青沼仁志, 杉本靖博, 細田耕, XUE Jianru 自律分散システム・シンポジウム(CD-ROM) 26th ROMBUNNO.1C3-3 2014年01月23日 [査読無し][通常論文]
  • 佐藤英毅, 加納剛史, 青沼仁志, 松坂義哉, 石黒章夫, 石黒章夫 自律分散システム・シンポジウム(CD-ROM) 26th ROMBUNNO.2A1-3 2014年01月23日 [査読無し][通常論文]
  • 矢野史朗, 渡邊崇之, 青沼仁志, 淺間一 自律分散システム・シンポジウム(CD-ROM) 26th ROMBUNNO.2D2-1 2014年01月23日 [査読無し][通常論文]
  • 青沼仁志 自律分散システム・シンポジウム(CD-ROM) 26th ROMBUNNO.WAKUSHOPPU,3 2014年01月23日 [査読無し][通常論文]
  • 青沼仁志 自律分散システム・シンポジウム(CD-ROM) 26th ROMBUNNO.1C3-1 2014年01月23日 [査読無し][通常論文]
  • Sakai, R., Shimizu, M., Aonuma, H., Hosoda, K. Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) 8608 LNAI 420 -422 2014年 [査読有り][通常論文]
  • Kuniaki Kawabata, Hitoshi Aonuma, Koh Hosoda, Yasuhiro Sugimoto, Jianru Xue 2014 IEEE INTERNATIONAL CONFERENCE ON ROBOTICS AND BIOMIMETICS IEEE-ROBIO 2014 949 -954 2014年 [査読有り][通常論文]
     
    This paper describes about experimental verifications on the cricket's response against frequent interactions and how it changes in time development. In order to investigate behavior modification, we took the approach to utilize automated interaction systems with a robotic agent and observe the reactions of the male cricket. The robotic agent robes cricket's pheromone and is controlled by designing motion patterns based on visual motion tracking of the both agents. We designed an automated interaction behavior pattern for the robotic agent and conducted interaction experiments to the male cricket. We observed that cricket showed ordinary response firstly and after a while the trend of the cricket's behavior changed. Thus, the response was somewhat modified in time development. In this paper, we explained experimental conditions and also showed the results of frequent interaction experiments by using a robotic agent.
  • 下地博之, 青沼仁志, 岡田泰和, 三浦徹, 辻和希 日本生態学会大会講演要旨(Web) 61st E1-12 (WEB ONLY) 2014年 [査読無し][通常論文]
  • 佐藤 英毅, 加納 剛史, 青沼 仁志, 松坂 義哉, 石黒 章夫 ロボティクス・メカトロニクス講演会講演概要集 2014 (0) _1A1 -V05_1-_1A1-V05_4 2014年 [査読無し][通常論文]
     
    Most robots are vulnerable to changes in their own morphology such as failures. To tackle this problem, we have focused on ophiuroids, which have simple body with pentaradial symmetry, as our model. Although they are composed of primitive decentralized nervous system, they can coordinate their arm movements adequately even when the arms are arbitrarily cut off. In this study, we aimed to clarify the fundamental mechanism underlying this resilient ophiuroid locomotion. For this purpose, we performed behavioral experiment using ohiuroids with different number of shortened arms, and proposed a simple autonomous decentralized control scheme on this basis. The validity of the propose control scheme was confirmed via simulation.
  • 坂井 亮, 清水 正宏, 青沼 仁志, 細田 耕 ロボティクス・メカトロニクス講演会講演概要集 2014 (0) _2A1 -J06_1-_2A1-J06_4 2014年 [査読無し][通常論文]
     
    Generation of adaptive swimming locomotion of Xenopus laevis is greatly affected by hydrodynamic interaction between its musculoskeletal system and water environments. To understand the mechanism of frog locomotion, it is a proper approach to build a hybrid musculoskeletal robot that has similar structure as a Xenopus laevis, and excite living muscles to drive the body like in vivo. We developed a swimming robot that had a similar musculoskeletal structure as a frog driven by living muscles. At first, we built musculoskeletal structure of the hind leg by a 3D printer copying the anatomy of Xenopus laevis. Then, we developed the swimming robot by driven the gastrocnemius muscles, realized kick motion that generated propulsion for swimming locomotion. And also, we checked that the robot's motion is similar to Xenopus laevis's one. Thus, to achieve to understand the swimming locomotion, the robot is expected to be a useful tool.
  • 松坂義哉, 佐藤英毅, 加納剛史, 坂本一寛, 青沼仁志, 石黒章夫 日本生理学雑誌 76 (1) 39 2014年01月01日 [査読無し][通常論文]
  • 矢野史朗, 渡邊崇之, 青沼仁志, 淺間一 計測自動制御学会システム・情報部門学術講演会講演論文集(CD-ROM) 2013 ROMBUNNO.SS14-8 2013年11月18日 [査読無し][通常論文]
  • Advanced statistical reach feature and its application to the cricket observation
    Y. Okuda, S. Takahashi, K. Kawabata, H. Aonuma, K. Iwata, Y. Satoh International Symposium on Optomechatronic Technologies 2013 (no. M1A-5) 6 pages 2013年10月 [査読有り][通常論文]
  • 青沼 仁志 比較生理生化学 30 (3) 87 -88 2013年09月20日 [査読無し][通常論文]
  • 青沼仁志, 渡邊崇之 日本動物学会大会予稿集 84th 136 2013年08月12日 [査読無し][通常論文]
  • 古橋一憲, 北川善政, 赤坂司, 亘理文夫, 青沼仁志 北海道外科雑誌 58 (1) 87 -88 2013年06月20日 [査読無し][通常論文]
  • 佐藤英毅, 加納剛史, 青沼仁志, 石黒章夫, 石黒章夫 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2013 ROMBUNNO.2A1‐O02 2013年05月21日 [査読無し][通常論文]
  • 清水正宏, 青沼仁志, 細田耕 自律分散システム・シンポジウム資料 25th 109 -112 2013年01月25日 [査読無し][通常論文]
  • Y. Okuda, S. Takahashi, K. Kawabata, H. Aonuma, K. Iwata, Y. Satoh 2013 FIRST INTERNATIONAL SYMPOSIUM ON COMPUTING AND NETWORKING (CANDAR) 324 -327 2013年 [査読有り][通常論文]
     
    Recently, research on the habit of creatures and their characteristics are widely based on image processing. For example, the cricket Gryllus bimaculatus which consist of micro brain modifies its behavior by the situation and experience. In this paper, we constructed a technique of image observation to detect a cricket based on an advanced statistical feature, and automatically generated action trajectory. Therefore, we are able to apply the simultaneous observation of crickets. Through experimentation, we proved the effectiveness of our proposed method.
  • Kobayashi M, Kataoka T, Aonuma H, Al-Mallahi A.A, Shibata Y IFAC Proceedings Volumes (IFAC-PapersOnline) 1 (PART 1) 2013年 [査読有り][通常論文]
  • Y. Okuda, S. Takahashi, K. Kawabata, H. Aonuma, K. Iwata, Y. Satoh 2013 IEEE INTERNATIONAL CONFERENCE OF IEEE REGION 10 (TENCON) 2013年 [査読有り][通常論文]
     
    Recently, research on the habit of creatures and their characteristics are widely based on image processing. Observing the behavior of Gryllus bimaculatus is important to understanding the ecology of their characteristics. Therefore, in this paper, we introduced an image processing algorithm and constructed a technique of image observation to detect crickets based on an advanced statistical reach feature, and generated action trajectories. Through experimentation, we proved the effectiveness of our proposed method.
  • Kuniaki Kawabata, Hitoshi Aonuma, Koh Hosoda, Jianru Xue 2013 IEEE International Conference on Robotics and Biomimetics, ROBIO 2013 1347 -1352 2013年 [査読有り][通常論文]
     
    This paper describes an automated interaction for triggering the cricket's pheromone behavior by controlling a micro mobile robot based on pose estimation. In our developing systems a micro mobile robot: Ecobe mounted a cricket s head is introduced as a physical agent to interact with the cricket. Both of location of the cricket and mobile robot is estimated by on-line particle filter based color tracking and the pose estimation is also done by utilizing visual processing. By utilizing extracted information and novel designed robot's behavior algorithm, automated experimental trials in interaction between a cricket and a micro mobile robot has been done. Experimental results by utilizing our prototype system are also reported in this paper. Finally, pose estimation was also applied to the cricket and we show the result of fundamental trial of that. © 2013 IEEE.
  • 加納剛史, 大須賀公一, 小林亮, 青沼仁志, 石川将人, 杉本靖博, 大脇大, 石黒章夫 交通流のシミュレーションシンポジウム論文集 19th 89 -92 2013年 [査読無し][通常論文]
  • 佐藤 英毅, 加納 剛史, 青沼 仁志, 石黒 章夫 ロボティクス・メカトロニクス講演会講演概要集 2013 (0) _2A1 -O02_1-_2A1-O02_4 2013年 [査読無し][通常論文]
     
    Most robots are vulnerable to the change in their own morphology such as failures. To tackle this problem, we focus on ophiuroids, which have simple body with pentaradial symmetry. Although they are composed of primitive decentralized nervous system, they can move by self-organizing their arm movements adequately even when the arms are arbitrarily cut off. As a first step, we concentrate on an ophiuroid with one arm and aim to clarify its locomotion mechanism. We propose a simple autonomous decentralized control scheme on the basis of behavioral experiments, and confirm its validity via simulations.
  • 佐藤英毅, 加納剛史, 坂本一寛, 松坂義哉, 青沼仁志, 石黒章夫 日本数理生物学会大会講演要旨集 23rd 172 2013年 [査読無し][通常論文]
  • 加納剛史, 大須賀公一, 小林亮, 青沼仁志, 石川将人, 杉本靖博, 大脇大, 石黒章夫 日本数理生物学会大会講演要旨集 23rd 166 2013年 [査読無し][通常論文]
  • 渡邉崇之, 青沼仁志 日本動物学会大会予稿集 83rd 163 2012年08月20日 [査読無し][通常論文]
  • 岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本動物学会大会予稿集 83rd 128 2012年08月20日 [査読無し][通常論文]
  • 佐倉緑, 山本泰生, 青沼仁志 日本動物学会大会予稿集 83rd 162 2012年08月20日 [査読無し][通常論文]
  • 川端邦明, 青沼仁志, 細田耕, XUE Jianru 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2012 ROMBUNNO.2P1-H05 2012年05月27日 [査読無し][通常論文]
  • Kuniaki Kawabata, Hitoshi Aonuma, Koh Hosoda, Jianru Xue 2012 IEEE INTERNATIONAL CONFERENCE ON ROBOTICS AND BIOMIMETICS (ROBIO 2012) 1615 -1620 2012年 [査読有り][通常論文]
     
    This paper describes about a prototype of active interaction experiment system between a cricket and an operated micro mobile robot and measured/collected data in real-time by using the system. The behavior selection of the cricket (Gyllus bimaculatus) is influenced by the experience or the context in living environment. Therefore, we are trying to investigate neuronal mechanisms underlying micro brain of the cricket. For gathering behavioral data, we are developing a control/measurement system for realizing active interaction experiment. The prototype is composed of a micro mobile robot as a physical interaction agent, a camera and a microphone and a computer commands to the micro mobile robot and record the data of video sequence, motion tracking and the audio. Experimental trial using the prototype was done and reported.
  • 川端 邦明, 青沼 仁志, 細田 耕, 薜 建儒 ロボティクス・メカトロニクス講演会講演概要集 2012 (0) _2P1 -H05_1-_2P1-H05_4 2012年 [査読無し][通常論文]
     
    This paper describes our trials of active interactions to the crickets by a micro mobile robot for attracting pheromone behaviors. Cricket Gyllus bimaculatus modifies its own behavior based on the experiences based on the pheromone interactions between the individuals. For active interaction experiments, the micro mobile robot is maneuvered by an operator via the computer and the partial body of the cricket is attached to the robot. In this report, we describe interaction experimental results between the cricket and micro mobile robot with the pheromone. The cricket shows different response to the male or female pheromone, respectively. The result shows this approach could contribute to the biology as a novel one from the viewpoint of setting up the experimental conditions of the interactions actively.
  • 川端邦明, 藤井喬, 鈴木剛, 青沼仁志, 太田順, 淺間一 日本機械学会論文集 C編(Web) 78 (792) 3028 -3032 2012年 [査読無し][通常論文]
     
    This paper discusses what is given to control of multi-agent system with dynamic behavior selection model which we already proposed. Cricket shows adaptive behavior modification in both of individual and group level based on individual interactions. Principle of such ability is useful to control multi-agent system by bottom-up approach. In this paper, we compare our proposed model with other behavior selection models in sweeping task and discuss its effects from computer simulation results.
  • T. Watanabe, H. Sadamoto, H. Aonuma INSECT MOLECULAR BIOLOGY 20 (5) 619 -635 2011年10月 [査読無し][通常論文]
     
    Serotonin (5-HT) modulates various aspects of behaviours such as aggressive behaviour and circadian behaviour in the cricket. To elucidate the molecular basis of the cricket 5-HT system, we identified 5-HT-related genes in the field cricket Gryllus bimaculatus DeGeer. Complementary DNA of tryptophan hydroxylase and phenylalanine-tryptophan hydroxylase, which convert tryptophan into 5-hydroxy-L-tryptophan (5-HTP), and that of aromatic L-amino acid decarboxylase, which converts 5-HTP into 5-HT, were isolated from a cricket brain cDNA library. In addition, four 5-HT receptor genes (5-HT(1A), 5-HT(1B), 5-HT(2 alpha), and 5-HT(7)) were identified. Expression analysis of the tryptophan hydroxylase gene TRH and phenylalanine-tryptophan hydroxylase gene TPH, which are selectively involved in neuronal and peripheral 5-HT synthesis in Drosophila, suggested that two 5-HT synthesis pathways co-exist in the cricket neuronal tissues. The four 5-HT receptor genes were expressed in various tissues at differential expression levels, suggesting that the 5-HT system is widely distributed in the cricket.
  • 矢野 史朗, 青沼 仁志, 淺間 一 インテリジェントシステム・シンポジウム講演論文集 2011 (21) 83 -86 2011年09月01日 [査読無し][通常論文]
     
    In the field of reinforcement learning, researchers have focused a role and dynamics of animal's neuromodulatory system, which is thought to correspond to meta-parameter of the learning system. Recently, the hypothesis is suggested and beginning to be verified that neuromodulator serotonin regulates the temporal discount factor. It is known that dominance hierarchy affects the amount of serotonin in animal society. It is also known that social hierarchy is generated from competitive behavior in a self-organizing manner. In this study, we propose self-organizing multi-agent reinforcement system which grows various temporal discount factors robust and fault-tolerant system. We show that this system has the capability adapting to environmental variability and robustness for vacancy or increasing of agents.
  • 岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本動物学会大会予稿集 82nd 145 2011年08月20日 [査読無し][通常論文]
  • 渡邊崇之, 青沼仁志 日本動物学会大会予稿集 82nd 100 2011年08月20日 [査読無し][通常論文]
  • 石川由希, 青沼仁志, 佐々木謙, 三浦徹 日本動物学会大会予稿集 82nd 144 2011年08月20日 [査読無し][通常論文]
  • 青沼仁志 日本動物学会大会予稿集 82nd 144 2011年08月20日 [査読無し][通常論文]
  • 佐倉緑, 渡邊崇之, 青沼仁志 日本動物学会大会予稿集 82nd 103 2011年08月20日 [査読無し][通常論文]
  • Tetsuro Funato, Masahito Nara, Daisuke Kurabayashi, Masatoshi Ashikaga, Hitoshi Aonuma JOURNAL OF EXPERIMENTAL BIOLOGY 214 (14) 2426 -2434 2011年07月 [査読無し][通常論文]
     
    Aggressive behaviour within pairs of male crickets leads to the establishment of a dominance hierarchy. Defeated males avoid their victorious adversaries for several hours before regaining aggressiveness. However, the defeated male does not regain aggressiveness if repeated fighting occurs. Loss of individual aggressiveness is limited by group size, which constrains the number of crickets fighting at any given time. Thus, group aggressive behaviour is modulated by an environmental factor, group size, which is ultimately determined by individual actions, i.e. fighting between two individuals. We developed a robot model to elucidate the mechanism of group-size-dependent behaviour alternation in crickets. The behaviour of individual robots was evaluated experimentally with mobile robots and the group behaviour of the robots was evaluated by computer simulation. We demonstrated that the group-size-dependent strategy in crickets could be generated by local interactions between robots, where the behaviour was governed by an oscillator and memory of the outcome of previous fights.
  • Ayako Wada-Katsumata, Ryohei Yamaoka, Hitoshi Aonuma JOURNAL OF EXPERIMENTAL BIOLOGY 214 (10) 1707 -1713 2011年05月 [査読無し][通常論文]
     
    In ants, including Formica japonica, trophallaxis and grooming are typical social behaviors shared among nestmates. After depriving ants of either food or nestmates and then providing them with either food or nestmates, a behavioral change in type and frequency of social interactions was observed. We hypothesized that starvation and isolation affected levels of brain biogenic amines including dopamine (DA) and octopamine (OA) - neuromediators modifying various insect behaviors - and tested the relationship between brain biogenic amines and social behaviors of stressed ants. Ants starved for 7. days contained lower brain DA levels and they did not perform trophallaxis toward nestmates. Feeding starved ants sucrose solution re-established trophallaxis but not brain DA levels. The performance of trophallaxis induced recovery of brain DA content to the level of untreated ants. Ants that were isolated for 2 days displayed markedly increased OA levels, which following nestmate interactions, returned to levels similar to those of control (non-isolated) ants and ants isolated for 1. h. We conclude that: (1) starvation reduced brain DA level but had no significant effect on brain OA (trophallaxis recovered the brain DA levels), and (2) isolation increased brain OA level but had no effect on brain DA (trophallaxis and grooming events recovered the brain OA levels). We suggest that social interactions with nestmates influenced brain biogenic amine homeostasis in stressed F. japonica.
  • 青沼仁志 日本生態学会大会講演要旨集 58th 146 2011年03月08日 [査読無し][通常論文]
  • 岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本動物学会中国四国支部会報 (63) 16 2011年 [査読無し][通常論文]
  • 矢野史朗, 渡邊崇之, 佐倉緑, 青沼仁志, 淺間一 計測自動制御学会システム・情報部門学術講演会講演論文集(CD-ROM) 2010 ROMBUNNO.2G2-2 2010年11月24日 [査読無し][通常論文]
  • 佐倉緑, 菊地美香, 青沼仁志 日本動物学会大会予稿集 81st 154 2010年08月20日 [査読無し][通常論文]
  • 岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本動物学会大会予稿集 81st 155 2010年08月20日 [査読無し][通常論文]
  • Rodrigo da Silva Guerra, Hitoshi Aonuma, Koh Hosoda, Minoru Asada Journal of Neuroscience Methods 191 (1) 138 -144 2010年08月 [査読無し][通常論文]
     
    This paper proposes a novel robot/insect mixed society setup which enhances the possibilities for insect behavioral research and can be used as a powerful tool for interdisciplinary studies on insect behavior. Micro-robots are equipped with decoys so as to allow a controlled dynamic interaction with crickets, Gryllus bimaculatus. A camera records the interaction and the video is later processed for the automatic tracking of each encounter between cricket and robot. A novelty of our method lies in using the robots as tools for the controlled evoking of specific insect behaviors rather than trying to build an insect-like robot. The possibility for performing controlled repeatable movements allows the stimulation of certain insect behaviors that are usually difficult to trigger using insects alone, allowing consistent behavioral research. A set of experiments were performed in order to validate the proposed setup. We also demonstrate the use of our setup for stimulating agonistic behavior during an electromyography recording session. © 2010 Elsevier B.V.
  • 藤井喬, 川端邦明, 青沼仁志, 佐倉緑, 鈴木剛, 太田順, 淺間一 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2010 ROMBUNNO.2A1-A01 2010年06月13日 [査読無し][通常論文]
  • 山崎まどか, 佐倉緑, 青沼仁志, 松山茂, 秋野順治, 山岡亮平 日本応用動物昆虫学会大会講演要旨 54th 115 2010年03月12日 [査読無し][通常論文]
  • 岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本比較生理生化学会大会予稿集 32nd 29 2010年 [査読無し][通常論文]
  • 岡田龍一, 池野英利, 赤松忠明, 岩田可南子, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本動物学会中国四国支部会報 (62) 12 2010年 [査読無し][通常論文]
  • 藤井 喬, 川端 邦明, 青沼 仁志, 佐倉 緑, 鈴木 剛, 太田 順, 淺間 一 ロボティクス・メカトロニクス講演会講演概要集 2010 (0) "2A1 -A01(1)"-"2A1-A01(4)" 2010年 [査読無し][通常論文]
     
    An insect generates an environmental adaptive behavior by a small brain. The authors think that such ability for environmental adaptation brings useful knowledge for behavior control of a multi-robot system. Therefore, ability for environmental adaptation of a cricket was modeled as a behavior selection model. Such ability for environmental adaptation of insects is not yet elucidated enough. Therefore, in this study, the authors discuss about an engineering application of proposed cricket's behavior selection model. In particular about this paper, proposed model was discussed and compared w...
  • J. Rob. Mech. 22 (4) 524 -531 2010年 [査読無し][通常論文]
  • 藤井 喬, 川端 邦明, 青沼 仁志, 鈴木 剛, 足利 昌俊, 太田 順, 浅間 一 ロボティクス・メカトロニクス講演会講演概要集 2009 (0) "2A1 -B10(1)"-"2A1-B10(2)" 2009年05月25日 [査読無し][通常論文]
     
    In this research we model a cricket's neuronal system of the adaptive behavior selection mechanism. In our model, it assumes that the biogenic amine(Octopamin) and efficacy to sensory input from an antenae to generate adaptive behavior. In this paper, we hypothesize that the internal state(Octopamine) regulates behavior transition probability in fighting behavior the computer simulations are done by using our model.
  • 藤井喬, 川端邦明, 青沼仁志, 鈴木剛, 足利昌俊, 太田順, 淺間一 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2009 ROMBUNNO.2A1-B10 2009年05月24日 [査読無し][通常論文]
  • 藤井喬, 川端邦明, 青沼仁志, 鈴木剛, 足利昌俊, 太田順, 淺間一 ロボティクスシンポジア予稿集 14th 35 -41 2009年03月16日 [査読無し][通常論文]
  • 山崎まどか, 佐倉緑, 青沼仁志, 松山茂, 秋野順治, 山岡亮平 日本応用動物昆虫学会大会講演要旨 53rd 54 2009年03月12日 [査読無し][通常論文]
  • Hosoda, K., Aonuma, H. Advanced Robotics 23 (5) 481 -482 2009年 [査読有り][通常論文]
  • Yano S, Ikemoto Y, Aonuma H, Asama H ICCAS-SICE 2009 - ICROS-SICE International Joint Conference 2009, Proceedings 2254 -2259 2009年 [査読有り][通常論文]
  • 岡田龍一, 赤松忠明, 岩田可南子, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本比較生理生化学会大会予稿集 31st 69 2009年 [査読無し][通常論文]
  • 佐倉緑, 岡田龍一, 青沼仁志 日本比較生理生化学会大会予稿集 31st 70 2009年 [査読無し][通常論文]
  • 石川由希, 青沼仁志, 佐々木謙, 三浦徹 日本比較生理生化学会大会予稿集 31st 71 2009年 [査読無し][通常論文]
  • Masatoshi Ashikaga, Midori Sakura, Mika Kikuchi, Tetsutaro Hiraguchi, Ryosuke Chiba, Hitoshi Aonuma, Jun Ota ADVANCED ROBOTICS 23 (5) 563 -578 2009年 [査読無し][通常論文]
     
    Agonistic behavior in crickets was investigated to understand socially adaptive behavior, which assists with the understanding of the design of an artificial autonomous system in a social organization. Agonistic behavior between male crickets is released by the perception of cuticular substances of conspecific males. The degree of aggressiveness in crickets escalates from antennal fencing to tactical contact until one male quits fighting, at which time social status is established. The question of whether crickets are able to recognize one another to establish social status in an agonist interaction is worthy of pursuit. However, it would be difficult to conduct an experiment to explore this issue while using many individuals. Hence, we examined the social organization among male crickets using a two-step approach: (i) an experiment involving a simulated cricket behavior model and (ii) an experiment involving a behavior experiment using real animals. Our results suggest that crickets establish social status without recognition of their opponents. (c) Koninklijke Brill NV, Leiden and The Robotics Society of Japan, 2009
  • H. Aonuma, Y. Kitamura, K. Niwa, H. Ogawa, K. Oka NEUROSCIENCE 157 (4) 749 -761 2008年12月 [査読無し][通常論文]
     
    The distribution of potential nitric oxide (NO) donor neurons and NO-responsive target neurons was revealed in the terminal abdominal ganglion (TAG) of the cricket. The expression of nitric oxide synthase (NOS) in the nervous system was examined by Western blotting using universal nitric oxide synthase (uNOS) antibody that gave about a 130 kDa protein band. Immunohistochemistry using the uNOS antibody detected neurons whose cell bodies are located at the lateral region of the TAG. These neurons expanded their neuronal branches into the dorsal-median region or the dorsal-lateral region of the TAG. NADPH-diaphorase histochemistry was performed to confirm the distribution of NOS-containing neurons. The distributions of cell bodies and stained neuronal branches were similar to those revealed by uNOS immunohistochemistry. NO-induced cGMP immunohistochemistry was performed to reveal NO-responsive target neurons. Most of the cell bodies stained by immunohistochemistry appeared at the dorsal side of the TAG. At the dorsal-median region, some unpaired neuronal cell bodies were strongly stained. Some efferent neurons whose axon innervate into each nerve root were strongly stained. The generation of NO in the TAG was detected by NO electrode. We found that NO is generally produced to maintain a basal concentration of 70 nM. Hemoglobin scavenged released NO from the ganglion. The concentration of NO was partly recovered when hemoglobin was replaced by normal saline. Application of 10 mu M L-arginine that is a substrate of NOS increased NO release by approximately 10 nM. Furthermore, an excitatory neurotransmitter acetylcholine (ACh) also increased NO generation by approximately 40-50 nM in concentration in addition to the basal level of 70 nM. Optical imaging with fluorescent NO-indicator demonstrated that ACh-induced enhancement of NO release was transiently observed in the outer-edge region of TAG, where cell bodies of NOS-immunoreactive neurons were located. These results suggest that ACh accelerates NO production via neuronal events activated by ACh in the TAG. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.
  • 青沼仁志, 佐倉緑, 足利昌俊, 藤木智久, 藤井喬, 川端邦明, 太田順, 淺間一 インテリジェント・システム・シンポジウム講演論文集 18th (CD-ROM) ROMBUNNO.E2-2 2008年10月23日 [査読無し][通常論文]
  • 岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本動物学会大会予稿集 79th 132 2008年08月20日 [査読無し][通常論文]
  • 佐倉緑, 頼経篤史, 青沼仁志 日本動物学会大会予稿集 79th 89 2008年08月20日 [査読無し][通常論文]
  • 舩戸 徹郎, 奈良 維仁, 倉林 大輔, 青沼 仁志, 足利 昌俊 ロボティクス・メカトロニクス講演会講演概要集 2008 (0) "2P2 -I13(1)"-"2P2-I13(4)" 2008年06月06日 [査読無し][通常論文]
     
    Crickets have a fixed habit to avoid the fighting for a certain minutes after their defeat. If the fighting opportunity is too frequent, some crickets cannot recover the militancy, which limits the number of fighting crickets depending on their group density. This phenomenon shows the property of crickets as an autonomous system that adapts to the density variation, i.e., environmental change, only by a local interaction. In this research, the mechanism of such a biological adaptability is discussed from the viewpoint of brain function. The behaviour selector is modelled using oscillator ne...
  • 藤井 喬, 川端 邦明, 青沼 仁志, 鈴木 剛, 足利 昌俊, 太田 順, 淺間 一 ロボティクス・メカトロニクス講演会講演概要集 2008 (0) "2P2 -I18(1)"-"2P2-I18(3)" 2008年06月06日 [査読無し][通常論文]
     
    Understanding the mechanism underlying insect's behavior selection leads to comprehend useful knowledge to design principle of adaptive system based on the interactions between the individuals. The aim of this research is to modelsocial-adaptive behavior mechanism focusing on the cricket's fighting behavior as one typical example of social adaptation. In the cricket's fighting behavior, the antennae play very important role and it is considerable as key element to emerge of social behavior adaptation. This paper describes to attempt to model the antennal information processing system based ...
  • 舩戸徹郎, 奈良維仁, 倉林大輔, 青沼仁志, 足利昌俊 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2008 ROMBUNNO.2P2-I13 2008年06月05日 [査読無し][通常論文]
  • 藤井喬, 川端邦明, 青沼仁志, 鈴木剛, 足利昌俊, 太田順, 淺間一 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2008 ROMBUNNO.2P2-I18 2008年06月05日 [査読無し][通常論文]
  • Tetsuro Funato, Daisuke Kurabayashi, Masahito Nara, Hitoshi Aonuma IEEE TRANSACTIONS ON SYSTEMS MAN AND CYBERNETICS PART B-CYBERNETICS 38 (3) 764 -770 2008年06月 [査読無し][通常論文]
     
    Insects have small brains, but their behavior is highly adaptive; this leads us to conclude that their brains possess a simple adaptation mechanism. This paper focuses on the pheromone processing of crickets, varying their aggression depending on their global neural connection, and proposes a behavior selection mechanism that can be controlled by network transformation. The controller is composed of an oscillator network, and its behavior is decided by the synchrony of organic oscillations. Furthermore, every network component corresponds to a certain brain module. A model is realized by using an analog circuit, and it is applied to a simple robot that displays the behavior of a real insect.
  • Oak Yono, Hitoshi Aonuma ZOOLOGICAL SCIENCE 25 (5) 517 -525 2008年05月 [査読無し][通常論文]
     
    Crickets respond to air currents with quick avoidance behavior. The terminal abdominal ganglion (TAG) has a neuronal circuit for a wind-detection system to elicit this behavior. We investigated neuronal transmission from cercal sensory afferent neurons to ascending giant interneurons (GIs). Pharmacological treatment with 500 mu M acetylcholine (ACh) increased neuronal activities of ascending interneurons with cell bodies located in the TAG. The effects of ACh antagonists on the activities of identified GIs were examined. The muscarinic ACh antagonist atropine at 3-mM concentration had no obvious effect on the activities of GIs 10-3, 10-2, or 9-3. On the other hand, a 3-mM concentration of the nicotinic ACh antagonist mecamylamine decreased spike firing of these interneurons. Immunohistochemistry using a polyclonal anti-conjugated acetylcholine antibody revealed the distribution of cholinergic neurons in the TAG. The cercal sensory afferent neurons running through the cercal nerve root showed cholinergic immunoreactivity, and the cholinergic immunoreactive region in the neuropil overlapped with the terminal arborizations of the cercal sensory afferent neurons. Cell bodies in the median region of the TAG also showed cholinergic immunoreactivity. This indicates that not only sensory afferent neurons but also other neurons that have cell bodies in the TAG could use ACh as a neurotransmitter.
  • 奈良維仁, 舩戸徹郎, 倉林大輔, 足利昌俊, 青沼仁志, 森山拓郎 ロボティクスシンポジア予稿集 13th 338 -343 2008年03月16日 [査読無し][通常論文]
  • 山崎まどか, 佐倉緑, 青沼仁志, 秋野順治, 山岡亮平 日本応用動物昆虫学会大会講演要旨 52nd 141 2008年03月12日 [査読無し][通常論文]
  • 山崎 まどか, 佐倉 緑, 青沼 仁志, 秋野 順治, 山岡 亮平 日本応用動物昆虫学会大会講演要旨 (52) 2008年03月12日 [査読無し][通常論文]
  • Hosoda, K., Aonuma, H. Advanced Robotics 22 (15) 1603 -1604 2008年 [査読有り][通常論文]
  • 山崎まどか, 佐倉緑, 青沼仁志, 秋野順治, 山岡亮平 日本比較生理生化学会大会予稿集 30th 27 2008年 [査読無し][通常論文]
  • 岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗 日本比較生理生化学会大会予稿集 30th 29 2008年 [査読無し][通常論文]
  • Ryuichi Okada, Hidetoshi Ikeno, Hitoshi Aonuma, Etsuro Ito ADVANCED ROBOTICS 22 (15) 1665 -1681 2008年 [査読無し][通常論文]
     
    A honeybee informs her nestmates of flower locations by a unique behavior called a 'waggle dance'. We regard this behavior as a good model of the 'propagation and sharing of knowledge' to maintain a society. We have attempted to reveal how this dance benefits the colony using mathematical models and computer simulation based on parameters obtained from observations of bee behavior. Our simulation indicated that the most successful forages were made by a putative bee colony that used the dance to communicate. Video analysis of worker honeybee behavior in the field showed that a bee does not dance in a single, random place in the hive, but waggles several times in one place and several times in another. The orientation and duration of waggle runs varied from run to run, within ranges of +/- 15 degrees and +/- 15%, respectively. We also found that most of the bees that listened to the waggle dance turned away from the dancer after listening to one or two runs. These data suggest that honeybees use the waggle dance as a method of communication, but that they must base their forages oil ambiguous information about the location of a food source. (C) Koninklijke Brill NV, Leiden and The Robotics Society of Japan, 2008
  • Midori Sakura, T. Hiraguchi, K. Ohkawara, H. Aonuma ACTA BIOLOGICA HUNGARICA 59 183 -187 2008年 [査読無し][通常論文]
     
    Pheromones are important cues for social insects such as ants. As a first step in elucidation of pheromonal information processing mechanisms in the myrmicine ant, we investigated the morphological structure of the antennal lobe. Using autoflourescence imaging, labeling of neuronal filamentous actin, and reduced silver impregnation staining, the antennal lobe was found to consist of five compartments that, each received input from a different antennal sensory tract. Two major tracts of projection neurons, the medial and lateral antenno-cerebral tract (m- and l-ACT), originated from a different region of the antennal lobe. The in-ACT originated from the posterior part of the antennal lobe whereas the I-ACT originated from the anterior part. These results demonstrate a spatial segregation of function within the antennal lobe.
  • R. Okada, H. Ikeno, Noriko Sasayama, H. Aonuma, D. Kurabayashi, E. Ito ACTA BIOLOGICA HUNGARICA 59 157 -162 2008年 [査読無し][通常論文]
     
    A honeybee informs her nestmates of the location of a flower she has visited by a unique behavior called a "waggle dance." On a vertical comb, the direction of the waggle run relative to gravity indicates the direction to the food source relative to the sun in the field, and the duration of the waggle run indicates the distance to the food source. To determine the detailed biological features of the waggle dance, we observed worker honeybee behavior in the field. Video analysis showed that the bee does not dance in a single or random place in the hive but waggled several times in one place and then several times in another. It also showed that the information of the waggle dance contains a substantial margin of error. Angle and duration of waggle runs varied from run to run, with the range of +/- 15 degrees and +/- 15%, respectively, even in a series of waggle dances of a single individual. We also found that most dance followers that listen to the waggle (lance left the dancer after one or two sessions of listening.
  • 岡田龍一, 池野英利, 青沼仁志, 倉林大輔, 伊藤悦朗 計測と制御 46 (12) 916 -921 2007年12月10日 [査読無し][通常論文]
  • 青沼 仁志, 長尾 隆司, 太田 順, 川端 邦明, 淺間 一 計測と制御 46 (12) 903 -909 2007年12月10日 [査読無し][通常論文]
  • 淺間 一, 青沼 仁志, 太田 順, 千葉 龍介 計測と制御 = Journal of the Society of Instrument and Control Engineers 46 (12) 885 -886 2007年12月10日 [査読無し][通常論文]
  • 淺間 一, 神崎 亮平, 青沼 仁志, 三浦 徹, 倉林 大輔, 太田 順 計測と制御 = Journal of the Society of Instrument and Control Engineers 46 (12) 951 -957 2007年12月10日 [査読無し][通常論文]
  • 小川 宏人, 馬場 欣哉, 北村 美一郎, 青沼 仁志, 岡 浩太郎 生物物理 47 (SUPPLEMENT_1) 2007年11月20日 [査読無し][通常論文]
  • Dai Hatakeyama, Hitoshi Aonuma, Etsuro Ito, Karoly Elekes BIOLOGICAL BULLETIN 213 (2) 172 -186 2007年10月 [査読無し][通常論文]
     
    We investigated the distribution and projection patterns of central and peripheral glutamate-like immunoreactive (GLU-LIR) neurons in the adult and developing nervous system of Lymnaea. Altogether, 50-60 GLU-LIR neurons are present in the adult central nervous system. GLU-LIR labeling is shown in the interganglionic bundle system and at the varicosities in neuropil of the central ganglia. In the periphery, the foot, lip, and tentacle contain numerous GLU-LIR bipolar sensory neurons. In the juvenile Lymnaea, GLU-LIR elements at the periphery display a pattern of distribution similar to that seen in adults, whereas labeled neurons increase in number in the different ganglia of the central nervous system from juvenile stage P1 up to adulthood. During embryogenesis, GLU-LIR innervation can be detected first at the 50% stage of embryonic development (the E50% stage) in the neuropil of the cerebral and pedal ganglia, followed by the emergence of labeled pedal nerve roots at the E75% stage. Before hatching, at the E90% stage, a few GLU-LIR sensory cells can be found in the caudal foot region. Our findings indicate a wide range of occurrence and a broad role for glutamate in the gastropod nervous system; hence they provide a basis for future studies on glutamatergic events in networks underlying different behaviors.
  • 舩戸徹郎, 奈良維仁, D’ANGELO A, PAGELLO E, 倉林大輔, 青沼仁志 日本ロボット学会学術講演会予稿集(CD-ROM) 25th 1D16 2007年09月13日 [査読無し][通常論文]
  • 川端邦明, 太田順, 青沼仁志, 淺間一 日本ロボット学会学術講演会予稿集(CD-ROM) 25th 1F14 2007年09月13日 [査読無し][通常論文]
  • 佐倉緑, 頼経篤史, 青沼仁志 日本動物学会大会要旨集 78th 100 2007年08月31日 [査読無し][通常論文]
  • 菊地美香, 足利昌俊, 太田順, 長尾隆司, 青沼仁志 日本動物学会大会要旨集 78th 99 2007年08月31日 [査読無し][通常論文]
  • 頼経篤史, 佐倉緑, 青沼仁志 日本動物学会大会要旨集 78th 101 2007年08月31日 [査読無し][通常論文]
  • 足利昌俊, 菊地美香, 平口鉄太郎, 佐倉緑, 千葉龍介, 青沼仁志, 太田順 インテリジェント・システム・シンポジウム講演論文集 17th 191 -196 2007年08月10日 [査読無し][通常論文]
  • 平口鉄太郎, 青沼仁志, 倉林大輔 インテリジェント・システム・シンポジウム講演論文集 17th 189 -190 2007年08月10日 [査読無し][通常論文]
  • Eriko Tsuji, Hitoshi Aonuma, Fumio Yokohari, Michiko Nishikawa Zoological Science 24 (8) 836 -849 2007年08月 [査読無し][通常論文]
     
    Social Hymenoptera such as ants or honeybees are known for their extensive behavioral repertories and plasticity. Neurons containing biogenic amines appear to play a major role in controlling behavioral plasticity in these insects. Here we describe the morphology of prominent serotonin-immunoreactive neurons of the antennal sensory system in the brain of an ant, Camponotus japonicus. Immunoreactive fibers were distributed throughout the brain and the subesophageal ganglion (SOG). The complete profile of a calycal input neuron was identified. The soma and dendritic elements are contralaterally located in the lateral protocerebrum. The neuron supplies varicose axon terminals in the lip regions of the calyces of the mushroom body, axon collaterals in the basal ring but not in the collar region, and other axon terminals ipsilaterally in the lateral protocerebrum. A giant neuron innervating the antennal lobe has varicose axon terminals in most of 300 glomeruli in the ventral region of the antennal lobe (AL) and a thick neurite that spans the entire SOG and continues towards the thoracic ganglia. However, neither a soma nor a dendritic element of this neuron was found in the brain or the SOG. A deutocerebral projection neuron has a soma in the lateral cell-body group of the AL, neuronal branches at most of the 12 glomeruli in the dorsocentral region of the ipsilateral AL, and varicose terminal arborizations in both hemispheres of the protocerebrum. Based on the present results, tentative subdivisions in neuropils related to the antennal sensory system of the ant brain are discussed. © 2007 Zoological Society of Japan.
  • H. Aonuma COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 148 S33 -S33 2007年08月 [査読無し][通常論文]
  • D. Kurabayashi, H. Aonuma, T. Funato, T. Fujiki, M. Ashikaga, J. Ota, H. Asama COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 148 S32 -S32 2007年08月 [査読無し][通常論文]
  • 川端 邦明, 藤木 智久, 青沼 仁志, 淺間 一 ロボティクス・メカトロニクス講演会講演概要集 2007 (0) "2A1 -A09(1)"-"2A1-A09(4)" 2007年05月11日 [査読無し][通常論文]
     
    This current research aims to model adaptive behavior selection in crickets fighting behavior from physiological knowledge. We have already proposed an action selection model by NO/cGMP cascade-OA model and it can be an adequate model for adaptive behavior selection in cricket's brain. In this report, we examine to model a condition for initiation/termination condition of fighting behavior after interaction between the crickets.
  • 川端邦明, 藤木智久, 青沼仁志, 淺間一 日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM) 2007 2A1-A09 2007年05月10日 [査読無し][通常論文]
  • Swidbert R. Ott, Hitoshi Aonuma, Philip L. Newland, Maurice R. Elphick JOURNAL OF COMPARATIVE NEUROLOGY 501 (3) 381 -399 2007年03月 [査読無し][通常論文]
     
    Nitric oxide (NO) is a diffusible signaling molecule with evolutionarily conserved roles in neural plasticity. Prominent expression of NO synthase (NOS) in the primary olfactory centers of mammals and insects lead to the notion of a special role for NO in olfaction. In insects, however, NOS is also strongly expressed in non-olfactory chemo-tactile centers of the thoracic nerve cord. The functional significance of this apparent association with various sensory centers is unclear, as is. the extent to which it occurs in other arthropods. We therefore investigated the expression of NOS in the pereopod ganglia of crayfish (Pacifastacus lenisculus and Procambarus clarkii). Conventional NADPH diaphorase (NADPHd) staining after formaldehyde fixation gave poor anatomic detail, whereas fixation in methanol/formalin (MF-NADPHd) resulted in Golgi-like staining, which was supported by immunohistochemistry using NOS antibodies that recognize a 135-kDa protein in crayfish. MF-NADPHd revealed an exceedingly dense innervation of the chemo-tactile centers. As in insects, this innervation was provided by a system of prominent intersegmental neurons. Superimposed on a putatively conserved architecture, however, were pronounced segmental differences. Strong expression occurred only in the anterior three pereopod ganglia, correlating with the presence of claws on pereopods one to three. These clawed pereopods, in addition to their role in locomotion, are crucially involved in feeding, where they serve both sensory and motor functions. Our findings indicate that strong expression of NOS is not a universal feature of primary sensory centers but instead may subserve a specific requirement for sensory plasticity that arises only in particular behavioral contexts.
  • Takayuki Watanabe, Mika Kikuchi, Dai Hatakeyama, Takumi Shiga, Takehiro Yamamoto, Hitoshi Aonuma, Masakazu Takahata, Norio Suzuki, Etsuro Ito DEVELOPMENTAL NEUROBIOLOGY 67 (4) 456 -473 2007年03月 [査読無し][通常論文]
     
    Nitric oxide (NO), hydrogen sulfide (H2S), and carbon monoxide (CO) are thought to act as gaseous neuromodulators in the brain across species. For example, in the brain of honeybee Apis mellifera, NO plays important roles in olfactory learning and discrimination, but the existence of H2S- and CO-mediated signaling pathways remains unknown. In the present study, we identified the genes of nitric oxide synthase (NOS), soluble guanylyl cyclase (sGC), cystathionine beta-synthase (CBS), and heme oxygenase (HO) from the honeybee brain. The honeybee brain contains at least one gene for each of NOS, CBS, and HO. The deduced proteins for NOS, CBS, and HO are thought to contain domains to generate NO, H2S, and CO, respectively, and to contain putative Ca2+/calmodulinbinding domains. On the other hand, the honeybee brain contains three subunits of sGC: sGC alpha 1, sGC beta 1, and sGC beta 3. Phylogenetic analysis of sGC revealed that Apis sGC alpha 1 and sGC beta 1 are closely related to NO- and CO-sensitive sGC subunits, whereas Apis sGC beta 3 is closely related to insect O-2-sensitive sGC subunits. In addition, we performed in situ hybridization for Apis NOS mRNA and NADPH-diaphorase histochemistry in the honeybee brain. The NOS gene was strongly expressed in the optic lobes and in the Kenyon cells of the mushroom bodies. NOS activity was detected in the optic lobes, the mushroom bodies, the central body complex, the lateral protocerebral lobes, and the antennal lobes. These findings suggest that NO is involved in various brain functions and that H2S and CO can be endogenously produced in the honeybee brain. (c) 2007 Wiley Periodicals, Inc.
  • 船戸 徹郎, 倉林 大輔, 奈良 維仁, 青沼 仁志 物性研究 87 (4) 579 -582 2007年01月20日 [査読無し][通常論文]
     
    この論文は国立情報学研究所の電子図書館事業により電子化されました。Insects have only a little brain but the behavior is highly adaptive. We consider that physical structure of the neural network works on the creation of the brain function and model the behavioral processor that controlled by its structural disposition. Nonlinear oscillator changes the behavior depends on the connection relationship, and the existence of oscillator and the function have been shown in antennal lobe(AL) of locust. Moreover, the structure of cricket AL changes by battle experience. Therefore, we model the function of AL using an oscillator netw...
  • 舩戸徹郎, 倉林大輔, 奈良維仁, 青沼仁志 物性研究 87 (4) 579 -582 2007年01月20日 [査読無し][通常論文]
  • Tetsuro Funato, Daisuke Kurabayashi, Masahito Nara, Hitoshi Aonuma 2007 IEEE INTERNATIONAL CONFERENCE ON ROBOTICS AND BIOMIMETICS, VOLS 1-5 1206 -+ 2007年 [査読有り][通常論文]
     
    Insects have only a small brain but their behaviour is highly adaptive; this adaptive feature leads us to expect their brain to possess a simple adaptation mechanism. This research focuses attention on the phenomenon of crickets varying their aggression depending on their global neural connection, and proposes a behaviour selection mechanism controlled by network transformation. The controller is composed of an oscillator network and its behaviour is decided by synchrony based on organic oscillation. Furthermore, every network component corresponds to a certain brain module. A model is realised using an analog circuit and it is applied to a simple robot model that displays the behaviour of a real insect.
  • 小川宏人, 馬藤欣哉, 北村美一郎, 青沼仁志, 岡浩太郎 日本比較生理生化学会大会予稿集 29th 25 2007年 [査読無し][通常論文]
  • 淺間 一, 青沼 仁志, 太田 順, 千葉 龍介 計測と制御 46 (12) 885 -886 2007年 [査読無し][通常論文]
  • 淺間 一, 神崎 亮平, 青沼 仁志, 三浦 徹, 倉林 大輔, 太田 順 計測と制御 46 (12) 951 -957 2007年 [査読無し][通常論文]
  • Role of NO signaling in switching mechanisms in the nervous system of insect
    Proc. SICE Ann. Conf. 2477 -2482 2007年 [査読無し][通常論文]
  • A study on neural circuit model of insects for adaptive behavior selection - Verification of action selection model in multi-individual environments -
    Proc. International Symp. Mobiligence 2 187 -190 2007年 [査読無し][通常論文]
  • 3-D atras of the cricket antennal lobe
    Proc. International Symp. Mobiligence 2 187 -190 2007年 [査読無し][通常論文]
  • Fighting experiences modulate aggressive and avoidance behaviors in crickets against male cuticular substances
    Proc. International Symp. Mobiligence 2 243 -246 2007年 [査読無し][通常論文]
  • Social experience dependent behavior selection in the cricket - from neuroethological approach to modeling -
    Proc. International Symp. Mobiligence 2 16 -19 2007年 [査読無し][通常論文]
  • Distributions of aminergic and nitric oxidergic neurons
    Proc. International Symp. Mobiligence 2 239 -242 2007年 [査読無し][通常論文]
  • Modeling of socially adaptive behavior in crickets
    Proc. International Symp. Mobiligence 2 191 -194 2007年 [査読無し][通常論文]
  • Foraging task of multiple mobile robots in a dynamic environment using adaptive behavior in crickets
    J. Robot. Mech. Accepted 2007年 [査読無し][通常論文]
  • A neuromodulation model for adaptive behavior selection of the cricket
    J. Robot. Mech. Accepted 2007年 [査読無し][通常論文]
  • Tetsutaro Hiraguchi, Masatoshi Ashikaga, Midori Sakura, Mika Kikuchi, Jun Ota, Hitoshi Aonuma ZOOLOGICAL SCIENCE 23 (12) 1203 -1203 2006年12月 [査読有り][通常論文]
  • Jun Murakami, Hitoshi Aonuma, Takashi Nagao ZOOLOGICAL SCIENCE 23 (12) 1190 -1190 2006年12月 [査読有り][通常論文]
  • Mamiko Suzuki, Tetsuya Kimura, Hiroto Ogawa, Hitoshi Aonuma, Yoshiichiro Kitamura, Kohji Hotta, Kotaro Oka ZOOLOGICAL SCIENCE 23 (12) 1187 -1187 2006年12月 [査読有り][通常論文]
  • Y. Kitamura, H. Aonuma, K. Oka, H. Ogawa COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 145 (3-4) 405 -406 2006年11月 [査読無し][通常論文]
  • Ogawa Hiroto, Kitamura Yoshiichiro, Aonuma Hitoshi, Oka Kotaro 生物物理 46 (SUPPLEMENT_2) 2006年10月01日 [査読無し][通常論文]
  • Akiko Wagatsuma, Sachiyo Azami, Midori Sakura, Dai Hatakeyama, Hitoshi Aonuma, Etsuro Ito JOURNAL OF NEUROSCIENCE RESEARCH 84 (5) 954 -960 2006年10月 [査読無し][通常論文]
     
    Interaction between the activator type of cyclic AMP response element binding protein (CREB1) and the repressor type (CREB2) results in determining the emergence of long-lasting synaptic enhancement involved in memory consolidation. However, we still do not know whether the constitutively expressed forms of CREB are enough or the newly synthesized forms are required for the synaptic enhancement. In addition, if the newly synthesized forms are needed, we must determine the time for translation of CREB from its mRNA. We applied the methods of RNA interference and real-time polymerase chain reaction (PCR) to CREB in the cerebral giant cells of Lymnaea. The cerebral giant cells play an important role in associative learning and employ a CREB cascade for the synaptic enhancement to neurons such as the B1 moto-neurons. We injected the small interfering RNA (siRNA) of CREB1 or CREB2 into the cerebral giant cells and examined the changes in amplitude of excitatory postsynaptic potential (EPSP) recorded in the B1 motoneurons. The changes in the amounts of CREB1 and CREB2 mRNAs were also examined in the cerebral giant cells. The EPSP amplitude was suppressed 15 min after injection of CREB1 siRNA, whereas that was augmented 60 min after injection of CREB2 siRNA. In the latter case, the decrease in the amount of CREB2 mRNA was confirmed by real-time PCR. Our results showed that the de novo synthesized forms of CREB are required within tens of minutes for the synaptic enhancement in memory consolidation. (c) 2006 Wiley-Liss, Inc.
  • Masazumi Iwasaki, Antonia Delago, Hiroshi Nishino, Hitoshi Aonuma ZOOLOGICAL SCIENCE 23 (10) 863 -872 2006年10月 [査読無し][通常論文]
     
    Male solitary animals frequently enter aggressive interactions with conspecific individuals to protect their territory or to gain access to females. After an agonistic encounter, the loser (subordinate individual) changes its behaviour from aggression to avoidance. We investigated agonistic interactions between pairs of male crickets to understand how dominance is established and maintained. Two naive males readily entered into agonistic interactions. Fights escalated in a stereotyped manner and were concluded with the establishment of dominance. If individuals were isolated after the first encounter and placed together 15 minutes later, subordinate crickets tended to avoid any further contact with the former dominant opponent. Moreover, subordinate males also avoided unfamiliar dominant and naive opponents. They displayed aggressive behaviour only towards unfamiliar subordinate opponents. This suggests that the subordinate male change their behaviour depending on the dominance status of the opponent. Dominant crickets, in contrast, displayed aggressive behaviour towards familiar as well as unfamiliar opponents. If the interval between the first and second encounter was longer than 30 minutes, the former subordinate male showed aggressive behaviour again. However, if the subordinate cricket was paired with the same opponent three consecutive times within 45 minutes, it avoided the former dominant opponent for up to 6 hours following the third encounter. Our results suggest that the maintenance of dominance in male crickets depends largely on the behavioural change of subordinate individuals. Possible mechanisms to maintain dominance are discussed.
  • 足利昌俊, 菊地美香, 平口鉄太郎, 佐倉緑, 青沼仁志, 太田順 インテリジェント・システム・シンポジウム講演論文集 16th 17 -22 2006年09月25日 [査読無し][通常論文]
     
    In this paper, we propose a model of foraging behavior in multiple mobile robots. In this model, robots select behavior using their activities, which adjusted by interaction between other robots. And this model is using a mechanism of fighting behavior in male crickets. We simulate this model, and which shows that this model is better than a model of multiple robots without their activities about the working efficiency at dynamical working space.
  • 藤木智久, 川端邦明, 池本有助, 青沼仁志, 淺間一 インテリジェント・システム・シンポジウム講演論文集 16th 23 -26 2006年09月25日 [査読無し][通常論文]
     
    Recent physiological research has revealed the importance of neuromodulator (e.g. NO) in the pheromone behavior such as fighting behavior in insects. This research aims to model the function of neuromodulator in fighting behavior of crickets, and to emerge adaptive action selection by constructive approach. In this report, we assume a model for adaptive behavior selection by NO/cGMP cascade according to the physiological knowledge, and run some computer simulations.
  • 舩戸徹郎, 倉林大輔, 奈良維仁, 青沼仁志 日本ロボット学会学術講演会予稿集(CD-ROM) 24th 1K33 2006年09月14日 [査読無し][通常論文]
  • Antonia Delago, Hitoshi Aonuma ZOOLOGICAL SCIENCE 23 (9) 775 -783 2006年09月 [査読無し][通常論文]
     
    Fighting behavior in male crickets is already well described, and some of the mechanisms underlying aggression and aggressive motivation have already been revealed. Much less is known about female/female interactions. Here, we report that adult female crickets that had been isolated for several days readily entered into agonistic interactions with conspecific individuals. Characteristic dyadic encounters between isolated females escalated in a stepwise manner and were concluded with the establishment of a dominant/subordinate relationship. For 15 to 30 minutes following an initial fight, former subordinate females showed a dramatic change in agonistic behavior. If they were paired with the former dominant opponent during this interval, a significant majority did not enter into any aggressive interaction but instead actively avoided the opponent. A similar experience-based and time-dependent increase in avoidance was observed when former subordinate females were paired with unfamiliar naive opponents. However, when faced with an unfamiliar subordinate individual in the second encounter, no such increase in avoidance behavior was observed. We propose that the observed changes in the behavior of former subordinate females are the consequence of a change in the general state of arousal and of the recognition of dominance status, but not of individual recognition. The fact that former dominant individuals did not show similar experience-based changes in agonistic behavior suggests that dominant/subordinate relationships between pairs of female crickets are maintained mainly by the behavior of subordinate individuals.
  • K Niwa, J Sakai, T Karino, H Aonuma, T Watanabe, T Ohyama, O Inanami, M Kuwabara FREE RADICAL RESEARCH 40 (2) 167 -174 2006年02月 [査読無し][通常論文]
     
    To elucidate the role of shear stress in fluid-phase endocytosis of vascular endothelial cells (EC), we used a rotating-disk shearing apparatus to investigate the effects of shear stress on the uptake of lucifer yellow (LY) by cultured bovine aortic endothelial cells ( BAEC). Exposure of EC to shear stress (area-mean value of 10 dynes/cm(2)) caused an increase in LY uptake that was abrogated by the antioxidant, N-acetyl-L-cysteine (NAC), the NADPH oxidase inhibitor, acetovanillone, and two inhibitors of protein kinase C (PKC), calphostin C and GF109203X. These results suggest that fluid-phase endocytosis is regulated by both reactive oxygen species (ROS) and PKC. Shear stress increased both ROS production and PKC activity in EC, and the increase in ROS was unaffected by calphostin C or GF109203X, whereas the activation of PKC was reduced by NAC and acetovanillone. We conclude that shear stress-induced increase in fluid-phase endocytosis is mediated via ROS generation followed by PKC activation in EC.
  • 足利 昌俊, 平口 鉄太郎, 佐倉 緑, 青沼 仁志, 太田 順 自律分散システム・シンポジウム資料 = SICE Symposium on Decentralized Autonomous Systems 18 189 -194 2006年01月26日 [査読無し][通常論文]
  • 佐倉緑, 平口鉄太郎, 大河原恭祐, 青沼仁志 日本比較生理生化学会大会予稿集 28th 38 2006年 [査読無し][通常論文]
  • 青沼仁志 日本比較内分泌学会大会及びシンポジウムプログラム・講演要旨 31st 15 2006年 [査読無し][通常論文]
  • Y Matsumoto, S Unoki, H Aonuma, M Mizunami LEARNING & MEMORY 13 (1) 35 -44 2006年01月 [査読無し][通常論文]
     
    Cyclic AMP pathway plays ail essential role in formation of long-term memory (LTM). In some species, the nitric oxide (NO)-cyclic GMP pathway has been found to act in parallel and complementary to the cAMP pathway for LTM formation. Here we describe a new role of the NO-cGMP pathway, namely, stimulation of the cAMP pathway to induce LTM. We have studied the signaling cascade Underlying LTM formation by systematically coinjecting various "LTM-inducing" and "LTM-blocking" drugs in crickets. Multiple-trial olfactory conditioning led to LTM that lasted for several days, while memory induced by single-trial conditioning decayed away within several hours. Injection of inhibitors of the enzyme forming NO, cGMP, or cAMP into the hemolymph prior to multiple-trial conditioning blocked LTM, whereas injection of ail NO donor, cGMP analog, or cAMP analog prior to single-trial conditioning induced LTM. Induction of LTM by injection of an NO donor or cGMP analog paired with single-trial conditioning was blocked by inhibitors of the cAMP pathway, but induction of LTM by a cAMP analog was unaffected by inhibitors of the NO-cGMP pathway. Inhibitors of cyclic nucleotide-gated channel (CNG channel) or calmodulin-blocked induction of LTM by cGMP analog paired with single-trial conditioning, but they did not affect induction of LTM by cAMP analog. Our findings suggest that the cAMP pathway is a downstream target of the NO-cGMP pathway for the formation of LTM, and that the CNG channel and calcium-calmodulin intervene between the NO-cGMP pathway and the cAMP pathway.
  • Aonuma Hitoshi, Kitamura Yoshiichiro, Ogawa Hiroto, Oka Kotaro Zoological science 22 (12) 2005年12月25日 [査読無し][通常論文]
  • Suzuki Mamiko, Kimura Tetsuya, Aonuma Hitoshi, Kitamura Yoshiichiro, Ogawa Hiroto, Hotta Kohji, Oka Kotaro Zoological science 22 (12) 2005年12月25日 [査読無し][通常論文]
  • Mamiko Suzuki, Tetsuya Kimura, Hitoshi Aonuma, Yoshiichiro Kitamura, Hiroto Ogawa, Kohji Hotta, Kotaro Oka ZOOLOGICAL SCIENCE 22 (12) 1489 -1489 2005年12月 [査読有り][通常論文]
  • Antonia Delago, Hitoshi Aonuma ZOOLOGICAL SCIENCE 22 (12) 1488 -1488 2005年12月 [査読有り][通常論文]
  • Jun Murakami, Hitoshi Aonuma, Takashi Nagao ZOOLOGICAL SCIENCE 22 (12) 1484 -1484 2005年12月 [査読有り][通常論文]
  • Hitoshi Aonuma, Yoshiichiro Kitamura, Hiroto Ogawa, Kotaro Oka ZOOLOGICAL SCIENCE 22 (12) 1481 -1481 2005年12月 [査読有り][通常論文]
  • Tetsutaro Hiraguchi, Hitoshi Aonuma ZOOLOGICAL SCIENCE 22 (12) 1481 -1481 2005年12月 [査読有り][通常論文]
  • 青沼 仁志 比較生理生化学 22 (3) 122 -123 2005年11月30日 [査読無し][通常論文]
  • 北村 美一郎, 青沼 仁志, 岡 浩太郎, 小川 宏人 生物物理 45 (SUPPLEMENT_1) 2005年10月19日 [査読無し][通常論文]
  • J Nagamoto, H Aonuma, M Hisada ZOOLOGICAL SCIENCE 22 (10) 1079 -1088 2005年10月 [査読無し][通常論文]
     
    Cuticular substances on the body surface of crickets serve as pheromones that elicit a variety of different behaviors in male crickets. Antennal contact between males and females resulted in courtship behavior, and that between two males resulted in aggressive displays. As a first step in elucidating how crickets recognize and discriminate individuals, behavioral responses of male individuals to cuticular substances of conspecific males or females were investigated. The behavioral responses of males to antennal or palpal stimulation with an isolated antenna from a male or a female were recorded. To both antennal and palpal stimulation with female antennae, the majority of males responded with courtship behavior; to stimulation with male antennae, males responded with aggressive displays. To gain insight into the chemical nature of the behaviorally relevant components, isolated antennae were washed in either n-hexane, acetone or ethanol before behavior assays. Washed antennae no longer elicited courtship or aggressive responses in males. Next, polypropylene fibers were smeared with substances from the body surface of females and used for antennal stimulation. This experiment showed that the quality and quantity of cuticular substances appear to be highly age-dependent. Significantly more males responded with courtship behavior to cuticular substances from younger females. Isolated males generally showed higher levels of aggression than males reared in groups. Grouped males also were more likely to display courtship behavior towards antennae from younger females, and aggressive behavior towards antennae from older females. These results suggest that male discrimination of mating partners depends on the nature of female cuticular substances.
  • 丹羽光一, 青沼仁志, 狩野猛 日本生理学雑誌 67 (3) 130 2005年03月01日 [査読無し][通常論文]
  • 青沼 仁志 日本ロボット学会誌 = Journal of Robotics Society of Japan 23 (1) 6 -10 2005年01月15日 [査読無し][通常論文]
  • 濱徳行, 高畑雅一, 青沼仁志 日本比較生理生化学会大会予稿集 27th 29 2005年 [査読無し][通常論文]
  • 渡邊崇之, 菊池美香, 青沼仁志, 鈴木範男, 伊藤悦朗 日本比較生理生化学会大会予稿集 27th 23 2005年 [査読無し][通常論文]
  • 西川道子, 辻衣里子, 青沼仁志, 横張文男 日本比較生理生化学会大会予稿集 27th 30 2005年 [査読無し][通常論文]
  • 平口鉄太郎, 青沼仁志 日本比較生理生化学会大会予稿集 27th 29 2005年 [査読無し][通常論文]
  • Pheromone processing center in the protocerebrum of Bombyx mori, J. Comp. Neurol., 481 : 340-351 (2005)*
    J. Comp. Neurol. 481 340 -351 2005年 [査読無し][通常論文]
  • Tetsutaro Hiraguchi, Yoshiichiro Kitamura, Koutarou Oka, Hitoshi Aonuma ZOOLOGICAL SCIENCE 21 (12) 1323 -1323 2004年12月 [査読有り][通常論文]
  • Masazumi Iwasaki, Chihiro Katagiri, Hitoshi Aonuma ZOOLOGICAL SCIENCE 21 (12) 1322 -1322 2004年12月 [査読有り][通常論文]
  • Hitoshi Aonuma, Masazumi Iwasaki ZOOLOGICAL SCIENCE 21 (12) 1321 -1321 2004年12月 [査読有り][通常論文]
  • Hitoshi Aonuma, Masazumi Iwasaki ZOOLOGICAL SCIENCE 21 (12) 1321 -1321 2004年12月 [査読無し][通常論文]
  • Masazumi Iwasaki, Chihiro Katagiri, Hitoshi Aonuma ZOOLOGICAL SCIENCE 21 (12) 1322 -1322 2004年12月 [査読無し][通常論文]
  • Tetsutaro Hiraguchi, Yoshiichiro Kitamura, Koutarou Oka, Hitoshi Aonuma ZOOLOGICAL SCIENCE 21 (12) 1323 -1323 2004年12月 [査読無し][通常論文]
  • 中山 由佳子, 河原 剛一, 青沼 仁志, 山内 芳子, 中島 崇行, 鉢呂 健 電子情報通信学会技術研究報告. MBE, MEとバイオサイバネティックス 104 (429) 41 -44 2004年11月08日 [査読無し][通常論文]
     
    単離した個々の新生ラットの培養心筋細胞は自発的な拍動を示し、細胞内Ca^<2+>濃度も拍動に伴って変化している(Ca^<2+>振動)。複数の心筋細胞からなる培養系は互いに同期した拍動を示すと共に、Ca^<2+>振動も同期している。本研究では、心筋細胞内Ca^<2+>振動の細胞間同期のメカニズムを明らかにすることを目的とした。培養開始から4日日においては、集塊中の心筋細胞ばかりでなく、集塊から離れた細胞のCa^<2+>振動も同期していた。ギャップ結合阻害薬の付加によっても同期は崩れず、またCx43に対する抗体を用いた免疫細胞化学的解析、Lucifer Yellowによるdye transfer解析によっても、集塊内の細胞と集塊から離れた細胞間でギャップ結合は未発達であることが推定された。Ca^<2+>振動の細胞間同期は拍動を停止させても持続したが、ATP受容体(purinoceptor)の阻害によって同期が崩れた。これらの実験結果は、心筋細胞内Ca^<2+>振動の細胞間同期に、ATP-purinoceptor系を介した細胞外情報伝達系が関与していることを示唆している。
  • Y Kitamura, H Aonuma, K Niwa, K Mizutani, H Ogawa, K Oka NITRIC OXIDE-BIOLOGY AND CHEMISTRY 11 (1) 106 -107 2004年08月 [査読無し][通常論文]
  • H Aonuma, M Iwasaki NITRIC OXIDE-BIOLOGY AND CHEMISTRY 11 (1) 107 -107 2004年08月 [査読無し][通常論文]
  • H Schuppe, M Araki, H Aonuma, T Nagayama, PL Newland ZOOLOGICAL SCIENCE 21 (1) 1 -5 2004年01月 [査読無し][通常論文]
     
    We have analysed the effects of the neuromodulator nitric oxide (NO) on proprioceptive information processing by ascending intersegmental interneurons that form part of the local circuits within the terminal abdominal ganglion of the crayfish. NO modulates the synaptic inputs to ascending interneurons, enhancing the amplitude of class I interneurons and reducing the amplitude of class 11 interneurons. Repetitive proprioceptive stimulation leads to rapid depression in a specific set of identified interneurons but not in others. Bath application of a nitric oxide scavenger, PTIO, causes a significant decrease in the rate of depression of the interneurons showing a rapid depression, independent of interneuron class, but has no effect on the dynamic responses of the interneurons that show little initial depression. These results indicate that NO exerts multiple effects at the very first stage of synaptic integration in local circuits.
  • H. Schuppe, M. Araki, H. Aonuma, T. Nagayama and P. L. Newland Effects of nitric oxide on proprioceptive signaling, Zool. Sci., 21(1): 1-5 (2004)*
    2004年 [査読無し][通常論文]
  • H. Aonuma, M. Iwasaki and K. Niwa Role of NO signaling in switching mechanisms in the nervous system of insect, Proceeding of SICE Annual Conference , 2004: 2477-2482 (2004)*
    2004年 [査読無し][通常論文]
  • T. Nagayama, k. Kimura, M. Araki, H. Aonuma and P. L. Newland Distribution of glutamatergic immunoreactive neurons in the terminal abdominal ganglion of the crayfish, J. Comp. Neurol., 474(1): 123-135 (2004)*
    2004年 [査読無し][通常論文]
  • S. Hisayo, H. Sato, S. Kobayashi, J. Murakami, H. Aonuma, H. Ando, Y. Fujito, K. Hamano, M. Awaji, K. Lukowiak, A. Urano and E. Ito CREB in the pond snail Lymnaea stagnalis -Cloning, gene expression and function in identifiable neurons of the central n・・・
    2004年 [査読無し][通常論文]
     
    S. Hisayo, H. Sato, S. Kobayashi, J. Murakami, H. Aonuma, H. Ando, Y. Fujito, K. Hamano, M. Awaji, K. Lukowiak, A. Urano and E. Ito CREB in the pond snail Lymnaea stagnalis -Cloning, gene expression and function in identifiable neurons of the central nervous system-, J. Neurobiol., 58(4): 455-466 (2004)*
  • H. Aonuma and K. Niwa Nitric oxide regulates the levels of cGMP accumulation in the cricket brain, Act. Biol. Hung., 55(1-4): 65-70 (2004)*
    2004年 [査読無し][通常論文]
  • Ogawa Hiroto, Kitamura Yoshiichiro, Hashii Hideaki, Mizutani Kenji, Aonuma Hitoshi, Oka Kotaro Zoological science 20 (12) 2003年12月25日 [査読無し][通常論文]
  • 青沼仁志, 丹羽光一 日本生理学雑誌 65 (12) 421 -422 2003年12月01日 [査読無し][通常論文]
  • 北村 美一郎, 橋井 秀明, 水谷 賢史, 青沼 仁志, 小川 宏人, 岡 浩太郎 生物物理 43 (SUPPLEMENT_1) 2003年08月25日 [査読無し][通常論文]
  • Matsumoto Y, Aonuma H, Mizunami M Zoological science 19 (12) 1476 -1477 2002年12月25日 [査読無し][通常論文]
  • Aonuma H, Matsumoto Y, Ott S.R, Elphick M.R, Mizunami M Zoological science 19 (12) 2002年12月25日 [査読無し][通常論文]
  • 青沼仁志 日本生理学雑誌 64 (1) 25 2002年01月01日 [査読無し][通常論文]
  • Aonuma, H. and Newland, P. L. (2002). Synaptic inputs onto spiking local interneurons in crayfish are depressed by nitric oxide. Journal of Neurobiology, 52, 144-55.*
    2002年 [査読無し][通常論文]
  • Fujie, S., Aonuma, H., Ito, I., Gelperin, A. and Ito, E. (2002). The nitric oxide/cyclic GMP pathway in olfactory processing system of the terrestrial slug Limax marginatus. Zoological Science, 19: 15-26.*
    2002年 [査読無し][通常論文]
  • 藤江紗代子, 青沼仁志, 伊藤伊織, 伊藤悦朗 日本神経科学大会プログラム・抄録集 24th 326 2001年09月26日 [査読無し][通常論文]
  • 藤江紗代子, 青沼仁志, 伊藤伊織, 伊藤悦朗 神経化学 40 (2/3) 382 2001年09月01日 [査読無し][通常論文]
  • 青沼 仁志 比較生理生化学 18 (2) 123 -125 2001年08月31日 [査読無し][通常論文]
  • H Schuppe, H Aonuma, PL Newland CELL AND TISSUE RESEARCH 303 (3) 451 -451 2001年03月 [査読無し][通常論文]
  • Aonuma, H. and Newland, P.L. (2001). Opposing actions of nitric oxide on synaptic inputs of identified interneurones in the central nervous system of the crayfish. Journal of Experimental Biology, 204: 1319-1332.*
    2001年 [査読無し][通常論文]
  • Aonuma, H. and Newland, P.L. (2001). The action of nitric oxide and cGMP on intersegmental interneurones in the crayfish terminal abdominal ganglion. in The Crustacean Nervous System. (Wiese K., ed.). Springer. P305-312.*
    2001年 [査読無し][通常論文]
  • H Schuppe, H Aonuma, PL Newland EUROPEAN JOURNAL OF NEUROSCIENCE 12 34 -34 2000年 [査読無し][通常論文]
  • H Aonuma, PL Newland EUROPEAN JOURNAL OF NEUROSCIENCE 12 34 -34 2000年 [査読無し][通常論文]
  • 青沼 仁志 比較生理生化学 16 (4) 297 -300 1999年12月31日 [査読無し][通常論文]
  • H Aonuma, T Nagayama JOURNAL OF COMPARATIVE NEUROLOGY 410 (4) 677 -688 1999年08月 [査読無し][通常論文]
     
    In the first step toward identifying the neurotransmitter released from spiking interneurons of both local and intersegmental groups in the crayfish terminal abdominal ganglion, the authors examined whether spiking local interneurons and ascending intersegmental interneurons contain the transmitter gamma-aminobutyric acid (GABA). In this paper, 17 identified ascending interneurons and three spiking local interneurons were stained by intracellular injection of Lucifer yellow and subsequently treated for immunocytochemical staining against GABA. Double-labeling experiments revealed that six identified ascending interneurons are GABAergic, but no spiking local interneurons show GABA-like immunoreactivity Four ascending interneurons with GABA-like immunoreactivity (reciprocal closing ascending neuron 5 [RC-5], reciprocal opening ascending neuron 6 [RO-6], variable-effect ascending interneuron 1 [VE-1], and no-effect ascending: interneuron 4[NE-4]) had cell bodies that formed a duster on the ventral surface of the rostral edge of the ganglion, whereas two GABAergic interneurons (coinhibiting ascending interneuron 2 [CI-2] and NE-2) had cell bodies in a caudal region around the cell body of the seventh flexor inhibitor (FI) motor neuron. Another four rostral interneurons (RC-2, RC-3, RC-4, and NE-3) and seven caudal interneurons (CI-3, RC-7, RO-1, RO-2, RO-3, RO-4, and NE-1) had no GABA-like immunoreactivity. Because VE-1 is known to make direct inhibitory connections with other ascending interneurons, whereas RC-3 and RO-1 are known to make direct excitatory connections, the immunocytochemical results from this study are consistent with previous physiological studies. Although many spiking local interneurons (including spiking local interneuron 1 of the anterior group [sp-ant1]) made direct inhibitory connections with nonspiking local interneurons, three spiking local interneurons (sp-ant1, spiking local interneuron 6 of the medial group [sp-med6], and spiking interneuron 5 of the posterior group [sp-post]) do not show GABA-like immunoreactivity. These results suggest that the inhibitory transmitter released from spiking local interneurons is not GABA but that another substance mediates the inhibitory action of these interneurons. (C) 1999 Wiley-Liss, Inc.
  • H Aonuma, T Nagao, T Nagayama, M Takahata JOURNAL OF EXPERIMENTAL ZOOLOGY 283 (6) 531 -540 1999年05月 [査読無し][通常論文]
     
    The modulatory effects of amino acids on neuromuscular transmission from an identified giant motor neuron (MoG) and abdominal fast flexor muscles of the crayfish were examined using electrophysiological and pharmacological techniques. The distribution of amino acids in the cell body and axon of a single MoG was revealed using high-performance liquid chromatography with electrochemical detection. Eight different amino acids-aspartate, glutamate, glutamine, arginine, glycine, taurine, alanaine and gamma-aminobutyric acid-were simultaneously detected in either the cell body or the axon of MoG. Aspartate, glutamate, alanine and arginine were present at relatively high concentration. Local pressure ejection of glutamate caused a depolarization of the abdominal fast flexor muscle fibers. On the other hand, aspartate, alanine and arginine had no clear effects on the same muscle fibers. Aspartate and arginine, however, had modulatory effects on neuromuscular transmission. Alanine had no significant effect on the neuromuscular transmission. Aspartate at a concentration of 200 mu M decreased the amplitude of EJPs in the fast flexor muscle mediated by stimulation of both the MoG and non-giant fast flexor motor neurons. Arginine at a concentration of 200 mu M reduced the EJP amplitude of the muscle fibers in response to MoG stimulation but enhanced the EJP amplitude of the same muscle fibers by stimulation of non-giant fast flexor motor neurons. Although rather high concentration (1 mM) were required, aspartate increased and arginine decreased the depolarization of the muscle fibers induced by local ejection of glutamate. The opposite effect of arginine on the fast flexor muscles in response to the stimulation of different motor neurons suggested its modulatory role in the different effects of these motor neurons (depression and facilitation) in the fast flexor muscles. J. Exp. Zool. 283:531-540, 1999. (C) 1999 Wiley-Liss, Inc.
  • H Aonuma, T Nagayama, M Takahata JOURNAL OF CRUSTACEAN BIOLOGY 18 (2) 243 -252 1998年05月 [査読無し][通常論文]
     
    Motor giant neurons (MoGs) of the crayfish are identifiable motor neurons that innervate abdominal fast flexor muscles. The role of L-glutamate as a neurotransmitter at the neuromuscular junction between MoG and the abdominal fast flexor muscles was investigated using electrophysiological and pharmacological techniques. Local application of L-glutamate to a fast flexor muscle induced a depolarization of the muscle fibers. This glutamatergic depolarization was dose-dependent and was associated with a conductance increase. Bath application of L-glutamate caused a reversible reduction in the amplitude of the excitatory junctional potentials (EJPs) of the fast flexor muscles evoked by MoG stimulation. Twenty micromole L-glutamate was sufficient to reduce the amplitude of MoG-evoked EJP. The effect of depression was dose-dependent and MoG stimulation elicited almost no response in flexor muscle fibers under 1 mM L-glutamate application. Joro spider toxin, an L-glutamate antagonist, reduced the amplitude of EJPs elicited by MoG stimulation at a concentration of 0.1 mu M. The response of the flexor muscle to MoG stimulation hid not recover completely after washing. When 5 mu M Joro spider toxin was applied, the EJP-evoked by MoG was completely blocked and no recovery was observed after washing. These results strongly suggest that L-glutamate is the neurotransmitter at the neuromuscular junction between MoG and the abdominal fast flexor muscle in the crayfish.
  • T Nagayama, H Namba, H Aonuma JOURNAL OF COMPARATIVE NEUROLOGY 389 (1) 139 -148 1997年12月 [査読無し][通常論文]
     
    The inhibitory neurotransmitter of premotor nonspiking local interneurones in the crayfish terminal abdominal ganglion was investigated physiologically and immunocytochemically. Depolarization of a nonspiking interneurone evoked a hyperpolarization in a uropod motor neurone. The amplitude of hyperpolarization in the motor neurone was gradually decreased under low-calcium/high-magnesium saline. Local pressure injection of gamma-aminobutyric acid (GABA) into the neuropil caused a similar hyperpolarization of the motor neurone. These physiological studies suggested a GABAergic inhibitory interaction between nonspiking interneurones and the motor neurones. Premotor nonspiking interneurones are classified into two subgroups of posterolateral (PL) and anterolateral (AL) interneurones, and AL interneurones are further divided into three subtypes. A combination of intracellular staining from nonspiking local interneurones with Lucifer yellow and immunocytochemical staining with an antiserum directed against GABA revealed that all the PL interneurones sampled in this study showed GABA-like immunoreactivity. A population of cell bodies (n = 6-11) with a small diameter (15-30 mu m) packed together forming a cluster showed GABA-like immunoreactivity, and the cell bodies of most PL interneurones were found in this cluster. To compare the number and the pattern of main branches of PL interneurones, cells were classified into three identifiable sets of interneurones, called PL-1, PL-2, and PL-3. By contrast, about one-half of AL interneurones, especially the third subtype of AL interneurones, which have cell bodies located ventrolaterally in the ganglion, did not show GABA-like immunoreactivity. Furthermore, the position of cell bodies of GABA-immunoreactive AL interneurones was scattered compared to that of PL interneurones. (C) 1997 Wiley-Liss, Inc.
  • 青沼仁志, 長山俊樹, 高畑雅一, ニューランド フィリップ 日本動物学会大会予稿集 68th 69 1997年10月 [査読無し][通常論文]
  • 長山俊樹, 青沼仁志, 難波寿明 日本動物学会大会予稿集 68th 69 1997年10月 [査読無し][通常論文]
  • 青沼仁志, 長山俊樹, 高畑雅一 日本動物学会大会予稿集 67th 74 1996年09月 [査読無し][通常論文]
  • H Aonuma, T Nagayama, M Takahata JOURNAL OF EXPERIMENTAL ZOOLOGY 275 (6) 406 -412 1996年08月 [査読無し][通常論文]
     
    Some central neurons in the crayfish have autofluorescent cell bodies. The yellow fluorescence of cell bodies was induced in the whole-mount preparation by using blue-violet light from a high-pressure mercury lamp. From the first to the fifth abdominal ganglion, a pair of cell bodies fluoresced in each segmental ganglion. In the sixth abdominal ganglion, two pairs of fluorescent cell bodies were detected. A pair of cell bodies also fluoresced in the posterior (fourth and fifth) thoracic ganglia, while the probability of finding fluorescent cell bodies in the anterior (first to third) thoracic ganglia was very low. Intracellular staining revealed 1) that autofluorescent cells were motor giants (MoGs) and 2) their homologue in the abdominal and thoracic ganglia. This characteristic of autofluorescence provides the cue of ready identification of particular crayfish central neurons, which are one of the most essential neural components for intrinsic escape behavior. (C) 1996 Wiley-Liss, Inc.
  • 青沼 仁志 比較生理生化学 12 (4) 425 -427 1995年12月27日 [査読無し][通常論文]
  • 青沼仁志, 長山俊樹, 高畑雅一, ニューランド フィリップ 日本動物学会大会予稿集 66th 102 1995年09月 [査読無し][通常論文]
  • 青沼仁志, 長山俊樹, 高畑雅一 日本動物学会大会予稿集 64th 54 1993年11月 [査読無し][通常論文]

受賞

  • 2017年06月 The 8th International Symposium on Adaptive Motion of Animals and Machines AMAM2017 Outstanding Demo Award
     Frog Cyborg Driven by Biological Muscle Actuators that Packaged Physiological Solution 
    受賞者: Shimizu M;Ishii D;Aonuma H;Hosoda K
  • 2013年09月 計測自動制御学会システム・情報部門 SSI Best Research Award
     クモヒトデのレジリアントなロコモーションに内在する自律分散制御則 
    受賞者: 佐藤英毅;加納 剛史;坂本 義哉;松坂 義哉;青沼 仁志;石黒 章夫
  • 2013年09月 日本数理生物学会 第23回日本数理生物学会大会 ポスター賞
     腕切断実験から探るクモヒトデの腕間協調メカニズム 
    受賞者: 佐藤英毅;加納 剛史;坂本 義哉;松坂 義哉;青沼 仁志;石黒 章夫
  • 2008年 日本動物学会 Zoological Science Award 2008
     Serotonin-immunoreactive neurons in the antennal sensory system of the brain in the carpenter ant, Camponotus japonicus 
    受賞者: Tsuji E;Aonuma H;Yokohari F;Nishikawa M
  • 2006年12月 計測自動制御学会システムインテグレーション部門講演会 SI2006優秀講演賞受賞
     コオロギ群の喧嘩行動をモデルとした動的環境下における移動ロボット群の採餌作業 
    受賞者: 青沼 仁志
  • 2006年09月 日本機械学会 2006年度日本機械学会フェロー賞
     昆虫の適応的行動選択を実現する神経回路モデルに関する研究(NO/cGMPカスケードによる適応的行動選択のモデル化) 
    受賞者: 藤木智久;川端邦明;池本有助;青沼仁志;淺間一
  • 2006年08月 第12回創発システム・シンポジウム「創発夏の学校」 ベストポスター賞
     コオロギ群の喧嘩行動を用いた移動ロボット群の採餌行動 
    受賞者: 足利昌俊;菊地美香;平口鉄太郎;佐倉緑;青沼仁志;太田順
  • 2002年08月 日本比較生理生化学会 吉田奨励賞
     節足動物の神経系における一酸化窒素の役割に関する研究 
    受賞者: 青沼 仁志

共同研究・競争的資金等の研究課題

  • 文部科学省:科学研究費補助金(挑戦的萌芽研究)
    研究期間 : 2014年 -2015年 
    代表者 : 青沼 仁志
  • 昆虫脳における適応的な行動制御信号の生成メカニズムの解明
    科学研究費補助金
    研究期間 : 2011年 -2013年
  • 文部科学省:科学研究費補助金(基盤研究(B))
    研究期間 : 2011年 -2013年 
    代表者 : 青沼 仁志, 細田 耕
     
    脳システムが,受容した感覚刺激から重要な情報を抽出し,その情報を経験や記憶と照合し,状況に応じた行動を発現するための運動制御信号をリアルタイムで生成する神経生理機構を明らかにする目的で,昆虫の闘争行動を題材に研究を進めた.コオロギは不完全変態昆虫で,発生後の生活様式を変えないまま幼虫から成虫にまで成長するため,各個体の経験や履歴を把握しながら実験が可能である.脳における適応行動の実時間制御の働き明らかにするため,闘争行動の過程で,一方の個体が負けを認めて攻撃を諦める過程の脳の働きを調べた.闘争行動の発現には,脳内の一酸化窒素(NO)シグナル伝達系と生体アミン類などの神経修飾物質の働きが重要である.そこで,これらの物質の作用ダイナミクスを明らかにするために,遺伝子改変コオロギの作成と脳の生理状態を自由行動個体で調べる方法の確立と改良を行った.個体行動を任意に操作するためには,コオロギサイズのロボットを使うので,ロボットシステムの改良を行った.また,自由行動個体からリアルタイムで生理機能を計測するシステムとしては,電気生理学的な方法とマイクロダイアリシス法を改良,確立した.一方,脳内神経修飾機構の機能阻害・賦活の為に,遺伝子改変コオロギを作成する法の確立を進めてきた.その結果,NO/cGMPシグナル伝達系関連遺伝子および,オクトパミン,セロトニン,ドーパミン等の生体アミン関連遺伝子の同定に成功した.また,遺伝子導入についても順調に進み,現在特定の遺伝子についての改変を行うことができる遺伝子導入法の確立を進めている.これらの研究成果により,2年目以降の研究を推進する上で非常に有効な技術開発ができた.
  • 文部科学省:科学研究費補助金(挑戦的萌芽研究)
    研究期間 : 2011年 -2012年 
    代表者 : 川端 邦明, 青沼 仁志
     
    研究課題の初年度である今年度は,昆虫と人工物の能動的相互作用による昆虫の行動切り替えメカニズム理解の基礎となる要件について研究開発を行った.具体的には,従来の生物学の知見から相互作用により行動切替を行う昆虫の典型例であるクロコオロギを対象として選び,小型移動ロボット(人工物)によるフェロモンコミュニケーションに基づいた能動的な相互作用による行動誘引および相互作用実験に関する条件を実験者の意図に基づいて操作・調整することが可能な実験環境の構築を行った.特に,計算機を用いた実験者による小型移動ロボットの赤外線通信による無線遠隔操縦システム,実験環境の上方に設置した俯瞰カメラから取得される相互作用実験中におけるクロコオロギおよび小型移動ロボットの行動・動作を動画データや音声データとしてオンラインで記録するシステムの開発を行った.クロコオロギ,小型移動ロボットの移動軌跡については,パーティクルフィルタを拡張した手法をソフトウェアとして開発,導入することでオンラインにトラッキング,記録することを可能とした.開発システムを用いて基礎的な検証実験を行った.また,クロコオロギの神経生理学的な研究を行うことで,次年度相互作用実験に必要となる知見・データを得た.今年度の研究活動によって得られた成果の一部については,国際学術雑誌において学術論文として採択された.また,国際・国内の学会などにおいて成果の公表を行うとともに周辺分野の研究者と議論を行った.
  • 文部科学省:科学研究費補助金(若手研究(B))
    研究期間 : 2009年 -2010年 
    代表者 : 定本 久世, 青沼 仁志, 濱 徳行, 小林 卓, 村上 準, 伊藤 悦朗, 藤戸 裕
     
    軟体動物モノアラガイでは、そしゃく行動に関連する神経回路が明らかにされている。本研究ではインシュリンによる体液性調節機構とそしゃく行動との関連を調べた。その結果、インシュリン様遺伝子発現量は、数日間の飢餓・飽食によっては変化せず、またそしゃく応答との関連はなかった。また、インシュリンに応答し、ショ糖刺激によって生理学的性質が変化するニューロン群はそしゃく行動に直接的に関わる神経節中に存在することが示された。
  • 文部科学省:科学研究費補助金(特定領域研究)
    研究期間 : 2005年 -2010年 
    代表者 : 淺間 一, 高草木 薫, 土屋 和雄, 伊藤 宏司, 矢野 雅文, 青沼 仁志, 大須賀 公一, 太田 順, 太田 順
     
    計画班および公募班が,本領域の特徴とする生工連携がスムーズにかつ効果的に行えるように,班間の連携を促進した.また,国際シンポジウム,ワークショップの開催,非公開シンポジウムの開催,内部評価の実施,国際会議・国内学会講演会などでのオーガナイズドセッションの企画,移動知教科書シリーズ出版企画,若手の会の支援,ホームページの更新,研究成果・活動記録に関するデータベースの作成などの広報,報告書の作成などを行った.
  • 文部科学省:科学研究費補助金(特定領域研究)
    研究期間 : 2005年 -2009年 
    代表者 : 青沼 仁志, 神崎 亮平
     
    社会環境への適応のメカニズムを明らかにするため,コオロギの闘争経験に基づく行動の選択の神経メカニズムを調査したところ,脳内の一酸化窒素・生体アミン系の働きが重要であることを突き止めた.また,行動と神経修飾機構を説明するモデルを構築し,シミュレーション実験と行動検定によりモデルの妥当性を検証した.その結果,個体間相互作用による部状態を更新するメカニズムが社会適応の実現に重要であることを明らかにした.
  • 文部科学省:科学研究費補助金(萌芽研究)
    研究期間 : 2005年 -2006年 
    代表者 : 下澤 楯夫, 青沼 仁志, 西野 浩史
     
    受精卵は卵割を繰り返して単細胞から多細胞へと移行する。受精卵の卵割を、単細胞生物から多細胞へと移行した進化過程のモデルとして扱い、多細胞化が、単細胞に比べて、熱雑音に埋もれた微弱な観測信号の信頼性(情報量)を加算平均化によって改善していることを実証する。これまでに、コオロギの気流感覚細胞は分子1個の平均運動エネルギー【approximately equal】k_BT(常温で約4×10〜<-21>Joule)に応答できることを実測し、この熱雑音感受性が神経系の基本構造である並列伝送回路の起源であることを示した。ここで重要なのは、単一分子の熱揺動エネルギーを検出する程の高感度は進化の結果ではなく拘束である、という結論にある。その根拠は、もし細胞がかって低かった感度を上げてk_BT領域に近づいたのなら、自ら熱雑音の中に埋もれて行ったことになり、観測装置としての自殺行為に他ならないからである。時間的空間的に偏った資源環境は、情報伝送速度を上げる向きの淘汰圧として働く。生命誕生前の原始のスープで利用可能なエネルギーは熱揺らぎ幅k_BTの程度であり、このk_BTの程度のエネルギー差を観測して情報(負のエントロピー)に変換する非平衡系が生命である。生命の起源にとって、k_BTの程度のエネルギー感度は必然である。細胞表面に複数のレセプター分子を並べることも加算平均化になる。観測装置としての細胞が複数結合した加算平均化が生存価を増す。多細胞生物への進化は、単細胞生物に比べて、熱雑音に埋もれた微弱な観測信号の信頼性(情報量)を加算平均化によって改善した例である、ことの情報理論的検証を目的とした。当初計画は、割球数の増加にともなう膜電位雑音の振幅減少の実測によって、加算平均原理を実証するものであった。しかし膜電位雑音の計測は不安定で卵割前後の比較が困難であった。また代替法として試みた膜電位固定下での電流雑音の計測も不調に終わった。このため、多細胞化の熱雑音仮説の実証は達成できなかったが、その情報論的考察の拡張に寄与した。
  • 文部科学省:科学研究費補助金(基盤研究(B))
    研究期間 : 2003年 -2005年 
    代表者 : 下澤 楯夫, 西野 浩史, 青沼 仁志
     
    神経系はなぜ多数の繊維からなる束なのか?多細胞生物としての当然の帰結なのか?これらの疑問は、つまるところ、神経系は進化の上での如何なる淘汰圧への適応の産物なのか、またその適応にはいかなる拘束条件が付きまとったのか、を問うことである。情報の生成(観測)にはエネルギー散逸が避けられず、感覚細胞における情報のエネルギーコストは統計熱力学上の理論限界である0.7K_BT[Joule/bit]に近い。本研究は、細胞の熱雑音感受性は進化を通して達成した適応ではなく、生命の起源に遡る拘束であることを明らかにし、資源や危害が時間的空間的に偏在する生存環境は情報伝送(観測)速度増大の淘汰圧として働くこと、それに対する唯一の適応方策は神経細胞の並列化であること、を次のように明らかにした。1)気流感覚毛で、揺動散逸定理に従ったブラウン運動を観察できることを光学計測によって示した。コオロギ尾葉上の近傍にある二つの気流感覚毛のブラウン運動の無相関性の計測は達成できなかった。2)気流感覚毛のブラウン運動と感覚細胞の電気的応答の相関(コヒーレンス)の実証には至らなかった。3)神経細胞は熱雑音領域で動作しており、情報伝送素子としての信号対雑音比が極めて低いことを、実証した。4)計測と平行して、信号対雑音比の極めて低い神経細胞のパルス列からでも、介在神経へのシナプス加重によって信号を再構成できることを理論的に示した。確率統計学や情報理論で、標本の平均値が母集団の真の平均値から外れる確率が標本数の平方根に反比例して少なくなる「加算平均原理」に着目し、熱雑音に拘束された細胞でも多数による加算平均によって、熱雑音以下の信号の検出精度が向上することを示した。もちろん「束」の前提として多細胞化は必要であるが、多細胞化の直接的生存価自体も、「加算平均原理」で説明できることを示した
  • 文部科学省:科学研究費補助金(基盤研究(B))
    研究期間 : 2003年 -2005年 
    代表者 : 水波 誠, 青沼 仁志, 松本 幸久
     
    近年、様々な動物で、学習・記憶に一酸化窒素(NO)を介した細胞間のシグナル伝達が重要な役割を果すことが報告されている。しかし、その仕組みを個体レベルでの行動と直接結びつけて解明する試みはほとんど行われていなかった。そこで本研究では、昆虫を材料に、長期記憶の形成にNOが果す役割について調べた。コオロギでは1回の嗅覚学習訓練(匂いと報酬の対呈示)により学習が成立するが、その記憶は数時間しか保持されない。一方、4回の学習訓練を行うと、学習後少なくとも4日間は保持される長期記憶が成立する。平成15年度の研究により、コオロギの学習訓練の前にNO合成阻害剤,NO除去剤,cGMP合成酵素阻害剤、cAMP合成酵素阻害剤,PKA阻害剤などを投与すると長期記憶の形成が阻害されること、NO発生剤(SNAP),膜透過型cGMP、透過型cAMPなどを投与すると長期記憶の誘導が起こることがわかった。平成16年度は、cGMP合成からcAMP合成に至るシグナル伝達経路について、長期記憶の阻害剤と誘導剤の系統的な組み合わせ投与によって調べた。その結果、NO-cGMP系とcAMP-PKA系の間をCNGチャネル(環状ヌクレオチド依存性陽イオンチャネル)およびカルシウム-カルモジュリン系が、介在していることが明らかになった。平成17年度は、cGMPがcAMP経路を介さず直接PKAに作用する経路が存在する可能性について検討した結果、匂い学習の際にはcGMPはPKAとは別のコンパートメントで発生するため、直接PKAには作用できないことがわかった。本研究により、動物の長期記憶に関わるシグナル伝達経路の全容が初めて明らかになった。
  • 文部科学省:科学研究費補助金(若手研究(A))
    研究期間 : 2002年 -2004年 
    代表者 : 青沼 仁志
     
    動物は、環境への適応機構のひとつとして神経系を進化の過程で獲得した。刻々と変化する状況に即して動物は様々な行動を発現し、外部環境の変化に適応している。では、動物は状況に応じてどの様な神経生理機構で適切な行動を発現し、切替えているのか?本研究では、いかに状況に応じた行動が発現されるのかその神経機構を理解しようとしている。神経の可塑的な性質は行動を切り換える際に必要な生理機構である。この様な神経の可塑的な変化には一酸化窒素(NO)が重要な役割を担っていると考え、節足動物、特にザリガニやコオロギを実験材料として、同定可能な神経回路網においてNOがどの様な修飾効果をもつのか細胞レベルでの詳細な解析を進めるとともに、行動レベルでの効果、役割を解析してきた。その成果として、1.組織化学染色法でNO産生細胞と標的細胞の局在をこれまでに確かめ、さらに、昆虫の脳では常にNOが100-200nM程度放出されていること、刺激により更に放出量が増加することを電極法で計測し示した。2.NOの光学的な計測を行い、中枢神経系の中でどの程度の一酸化窒素が産生されているのか突き止めるため、既存の共焦点レーザー走査顕微鏡を改造するため備品としてレーザー光源ユニットを購入し、NO感受性色素を使って光学的に解析を続けている。3.電気生理学的にNO産生細胞と標的細胞の両方の細胞から活動を計測し、一酸化窒素やcGMPの合成を促進或いは抑制する薬物を灌流投与し、一酸化窒素の修飾効果を解析し、神経系におけるNOの修飾効果を示した。これまでの成果は国際シンポジウム及び国内学会で発表し、また、その一部は既に国際学会誌等に論文として発表し、残りの成果については現在発表の準備に取りかかっている。
  • 文部科学省:科学研究費補助金(萌芽研究)
    研究期間 : 2002年 -2003年 
    代表者 : 下澤 楯夫, 水波 誠, 青沼 仁志, 西野 浩史
     
    神経細胞は熱雑音感受性をもっており、そのことが神経系に共通する基本構造である並列伝送回路の起源であることを、1.神経細胞は情報受容に当たってエネルギーを散逸する抵抗体として動作し、揺動散逸定理に従ったブラウン運動を観察できること、2.異なる細胞のブラウン運動は互いに無相関であること、3.感覚細胞がブラウン運動を用いて確率サンプリングした信号は介在神経でシナプス加算によって再構成されること、を実証するため研究を進めてきた。コオロギ気流感覚毛の機械特性の計測から感覚閾値で感覚細胞が受け取るエネルギーが常温における分子1個の熱揺動エネルギーk_BTに近いこと、閾値付近の刺激に対する応答発火揺らぎの解析から感覚細胞は内部の熱雑音源をパルス符号化に用いるStochastic Samplingで中枢へ信号を送っていること、感覚細胞の情報伝送容量の測定から、信号対雑音比は0.1程度と非常に低いこと、このため情報伝送素子としての細胞1個の性能は400ビット/秒程度に制限されている。これまでの研究で、気流感覚毛先端のブラウン運動が10nm程度であることを予想して、光学顕微鏡を改造し感覚毛のレーザー照明強拡大像(900倍)を4分割受光素子上に結ばせたブラウン運動計測装置(分解能3nmでの計測が可能)を実用化(平成12年度基盤研究(B)で製作)し、コオロギ気流感覚毛のブラウン運動を光学的に測定した。現在引き続き、2本の気流感覚毛のブラウン運動を同時計測し、近傍のものでも感覚細胞は互いに無相関な熱雑音に曝されていることを実証すべく解析を続けている。
  • 文部科学省:科学研究費補助金(基盤研究(B))
    研究期間 : 2000年 -2002年 
    代表者 : 下澤 楯夫, 馬場 欣哉, 青沼 仁志, 西野 浩史, 水波 誠, 馬場 欣也
     
    機械受容は、動物と外界との相互作用の「基本要素」であり、機械刺激の受容機構を抜きにして動物の進化・適応は語れない。従来、機械受容は「膜の張力によるイオンチャネルの開閉」といった「マクロで単純すぎる」図式でとらえられて来た。また、機械感覚の超高感度性の例として、ヒトやクサカゲロウの聴覚閾値での鼓膜の変位量が0.1オングストローム、つまり水素原子の直径の1/10に過ぎないことも、数多く示されてきた。しかし、変位で機械感度を議論するのは明らかに誤っている。感覚細胞は外界のエネルギーを情報エントロピーに変換する観測器であり、その性能はエネルギー感度で表現すべきである。エネルギーの授受無しの観測は「Maxwellの魔物」で代表される統計熱力学上の矛盾に行き着くから、いかなる感覚細胞も応答に際し刺激からエネルギーを受け取っている。コオロギの気流感覚細胞は、単一分子の常温における熱搖動ブラウン運動)エネルギーkBT(300°Kで4×10^<-21>[Joule])と同程度の刺激に反応してしまう。機械エネルギーが感覚細胞の反応に変換される仕組み、特にその初期過程は全く解明されていない。この未知の細胞機構を解明するため、ブラウン運動に近いレベルの微弱な機械刺激を気流感覚毛に与えたときの感覚細胞の膜電流応答の計測に、真正面から取り組んだ。長さ約1000μmのコオロギ気流感覚毛を根元から100μmで切断し、ピエゾ素子に取付けた電極を被せてナノメートル領域で動かし、気流感覚細胞の膜電流応答を計測した。長さ1000μmの気流感覚毛の先端は、ブラウン運動によって約14nm揺らいでいることは計測済みである。先端を切除した気流感覚毛を10-100nmの範囲で動かしたときの膜電流応答のエネルギーを計測し、刺激入力として与えた機械エネルギーと比べたところ、すでに10^6倍ほどのエネルギー増幅を受けていた。従って、機械受容器の初期過程は細胞膜にあるイオンチャンネルの開閉以前の分子機構にあることが明らかとなった。
  • 文部科学省:科学研究費補助金(特定領域研究(A))
    研究期間 : 2000年 -2001年 
    代表者 : 下澤 楯夫, 馬場 欣哉, 青沼 仁志, 西野 浩史
     
    昆虫の機械受容器は、常温の熱揺動エネルギーk_BT(約4×10^<-21>J、可視光フォトンのエネルギーの1/100)をも検出できる最高度に進化した刺激受容系である。コオロギ気流感覚細胞が熱雑音(ブラウン運動)に揺すられて発火するほど高感度なこと、中枢の介在神経は熱雑音の無相関性を利用して閾値以下の微弱な信号を抽出することを明らかにする目的で本プロジェクトを遂行した。フォトンのような量子を持たない機械受容器、化学受容器、イオンチャンネル、シナプス受容体などは必然的に熱雑音に出会うから、雑音揺らぎやシナプス加重機構が神経系一般に広くみられることなど、情報機械としての神経系が究極の乱数発生器としての熱雑音を利用する設計原理の解明につながる。コオロギを実験材料に、気流感覚器の機械的構造と感覚細胞のエネルギー変換応答とを分離し、分子熱雑音限界までエネルギー感度を進化させた機械感覚細胞の刺激受容機構解明の手がかりを得、情報機械としての感覚細胞の性質を解明するため感覚細胞が運ぶ情報、感覚細胞が介在神経に伝える情報量の定量化を試みた。中枢の介在神経から記録をとり、閾値付近の気流刺激への応答から、感覚細胞から介在神経への情報伝送量を測定した。コオロギの気流感覚細胞が運ぶ情報量はスパイク1つ当たり2〜4bit。入力感覚繊維1本あたりの情報量はおよそ300bits/secであることを示した。また、中枢の気流感覚介在神経の樹状突起部から細胞内記録をとり、閾値付近の人口白色雑音波形を繰り返し与え、後シナプス集合電位の変動分から、気流から介在神経までの情報伝送量(bits/sec)を測定した結果、50〜200bits/sec程度とばらつきはあるが1個の感覚細胞が伝える情報量より遥かに小さいことがわかった。昆虫の機械受容器は究極感度まで進化し熱雑音に直面している、逆説的に熱雑音のエネルギーと無相関性を利用してさらに微弱な信号を検出する中枢機構が進化したことが示唆された。
  • 行動切替えの神経機構に関する研究

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主要な担当授業

  • 行動システム制御科学特論
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 生命科学院
    キーワード : 行動,脳,中枢神経系,社会性,コミュニケーション,認知,神経回路,ニューロン,最初期遺伝子,感覚情報処理,運動制御,学習,進化
  • 大学院共通授業科目(一般科目):自然科学・応用科学
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 大学院共通科目
    キーワード : 生命システム, 生命機能, 研究方法論, 研究技術論
  • 生命システム科学基礎論
    開講年度 : 2018年
    課程区分 : 修士課程
    開講学部 : 生命科学院
    キーワード : 生命システム, 生命機能, 研究方法論, 研究技術論


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