研究者データベース

野中 成晃(ノナカ ナリアキ)
獣医学研究院 獣医学部門 病原制御学分野
教授

基本情報

所属

  • 獣医学研究院 獣医学部門 病原制御学分野

職名

  • 教授

学位

  • 博士(Ph.D.)(米国ミシガン州立大学)
  • 獣医学修士(北海道大学)

ホームページURL

J-Global ID

研究キーワード

  • 疫学   臨床寄生虫病学   寄生虫学   Epidemiology   Clinical Parasitology   Parasitology   

研究分野

  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 動物生産科学
  • ライフサイエンス / 動物生産科学

職歴

  • 2004年 - 現在 北海道大学講師
  • 1996年 - 2004年 北海道大学 助手
  • 1996年 - 2004年 Instructor of Hokkaido University
  • 2004年 - Lecturer of Hokkaido University
  • 1993年 - 1996年 日本学術振興学 特別研究員
  • 1993年 - 1996年 Research fellow of the Japan Society for the
  • Scientists
  • Promotion of Science for Japanese Junior
  • 宮崎大学 農学部 獣医学科 講師

学歴

  •         - 1993年   ミシガン州立大学大学院   獣医学研究科   大動物臨床学
  •         - 1993年   Michigan State University
  •         - 1985年   北海道大学   獣医学部   獣医学
  •         - 1985年   北海道大学

所属学協会

  • 日本寄生虫学会北日本支部会   世界獣医寄生虫学会(World Association for the Advanced Veterinary Parasitologist)   日本寄生虫学会   日本獣医寄生虫学会   米国獣医寄生虫学会(American Association of Veterinary Parasitologist)   日本獣医学会   

研究活動情報

書籍

  • Effective countermeasures against alveolar echinococcosis in red fox population of Hokkaido, Japan
    Echinococcosis in central Asia: Problems and solutions, Publishing house "Dauir", Zurich/Almaty 2004年
  • Ⅶ人獣共通寄生虫症 6.回虫症,人獣共通感染症
    医薬ジャーナル社,大阪 2004年
  • Ⅵ原虫性人獣共通感染症 8.ジアルジア症,人獣共通感染症
    医薬ジャーナル社,大阪 2004年
  • コクシジウム症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • トキソプラズマ症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • ジアルジア症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 回虫症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 鉤虫症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 耳疥癬,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 主要な消化管内寄生虫病一覧,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 鉤虫症,共通感染症ハンドブック
    日本農村情報システム協会,東京 2004年
  • Effective countermeasures against alveolar echinococcosis in red fox population of Hokkaido, Japan
    Echinococcosis in central Asia: Problems and solutions, Publishing house "Dauir", Zurich/Almaty 2004年
  • Ⅶ人獣共通寄生虫症 6.回虫症,人獣共通感染症
    医薬ジャーナル社,大阪 2004年
  • Ⅵ原虫性人獣共通感染症 8.ジアルジア症,人獣共通感染症
    医薬ジャーナル社,大阪 2004年
  • コクシジウム症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • トキソプラズマ症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • ジアルジア症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 回虫症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 鉤虫症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 耳疥癬,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 主要な消化管内寄生虫病一覧,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 2004年
  • 鉤虫症,共通感染症ハンドブック
    日本農村情報システム協会,東京 2004年
  • Control and management of parasitic zonooses maintained in wildlife: A trial of Hokkaido University against echinococcosis
    Technology innovation and its relations to humanities and social sciences 2003年
  • Control and management of parasitic zonooses maintained in wildlife: A trial of Hokkaido University against echinococcosis
    Technology innovation and its relations to humanities and social sciences 2003年
  • Helminths of Wildlife. 12. Japan
    Science Publishers Inc., Enfield(USA) 2001年
  • Helminths of Wildlife. 12. Japan
    Science Publishers Inc., Enfield(USA) 2001年
  • A diagnostic method for the definitive host of Echinococcus multilocularis by coproantigen detection
    Alveolar Echinococcosis ; Strategy for Eradication of Alveolar Echinococcosis of the Liver (Fujishoin) 1996年
  • A diagnostic method for the definitive host of Echinococcus multilocularis by coproantigen detection
    Alveolar Echinococcosis ; Strategy for Eradication of Alveolar Echinococcosis of the Liver (Fujishoin) 1996年

作品等

  • 中南米におけるテニア科条虫の疫学調査
    1995年 -2000年
  • Epidemiology of taeniid cestodes in Latin America
    1995年 -2000年
  • エキノコックス終宿主診断法の開発
    1997年 -1999年
  • Development of a diagnostic method for the definitive host of Echinococcus
    1997年 -1999年
  • ウルグアイ・アルゼンチンにおける単包条虫の疫学調査
    1999年
  • ホンジュラス・オランチョ県におけるブタ有鉤嚢虫の疫学調査
    1999年
  • Epidemiological Study of Echinococcus granulosus in Uruguay and Argentina
    1999年
  • A survey of Taenia solium cysticercosis in pigs in Orancho, Honduras
    1999年
  • エキノコックス症感染源対策
    1997年
  • ブラジル・サルバドールにおけるブタ有鉤(]J2276[)虫感染の疫学調査
    1997年
  • Control for the definitive hosts of Echinococcus
    1997年
  • A survey of Taenia solium cysticercosis in pigs in Salvador, Brazil
    1997年
  • ウルグアイにおける単包条虫終宿主に対する診断法の開発と疫学調査
    1995年
  • Development of diagnostic method for the definitive host of Echinococcus granulosus and its application to epidemiological study
    1995年
  • アラスカにおける多包条虫疫学調査
    1989年
  • Epidemiological Study of Echinococcus multilocularis in Alaska
    1989年

その他活動・業績

  • SL Chang, N Nonaka, M Kamiya, Y Kanai, HK Ooi, WC Chung, Y Oku PARASITOLOGY RESEARCH 96 (2) 95 -101 2005年05月 [査読無し][通常論文]
     
    Development of Taenia saginata asiatica metacestodes in SCID mice, and its infectivity in humans, golden hamsters, and Mongolian gerbils as alternative definitive hosts, were investigated. Cysticerci were recovered from SCID mice that were subcutaneously injected with hatched oncospheres of T. s. asiatica. The morphological changes of metacestodes were observed. The recovered cysticerci were fed to gerbils, hamsters and humans, to check for their infectivity. Tapeworms were recovered from gerbils and hamsters fed with 20 to 45 week-old cysticerci, and proglottids excretions were observed in human volunteers fed with 45 week-old cysticerci. However, no tapeworms were recovered from gerbils fed with 10 week-old cysticerci. Our results suggest that T. s. asiatica oncospheres needed more than 20 weeks to develop to maturity in SCID mice to be infective to both their natural and alternative definitive hosts.
  • SL Chang, N Nonaka, M Kamiya, Y Kanai, HK Ooi, WC Chung, Y Oku PARASITOLOGY RESEARCH 96 (2) 95 -101 2005年05月 [査読無し][通常論文]
     
    Development of Taenia saginata asiatica metacestodes in SCID mice, and its infectivity in humans, golden hamsters, and Mongolian gerbils as alternative definitive hosts, were investigated. Cysticerci were recovered from SCID mice that were subcutaneously injected with hatched oncospheres of T. s. asiatica. The morphological changes of metacestodes were observed. The recovered cysticerci were fed to gerbils, hamsters and humans, to check for their infectivity. Tapeworms were recovered from gerbils and hamsters fed with 20 to 45 week-old cysticerci, and proglottids excretions were observed in human volunteers fed with 45 week-old cysticerci. However, no tapeworms were recovered from gerbils fed with 10 week-old cysticerci. Our results suggest that T. s. asiatica oncospheres needed more than 20 weeks to develop to maturity in SCID mice to be infective to both their natural and alternative definitive hosts.
  • N Kato, N Nonaka, Y Oku, M Kamiya PARASITOLOGY RESEARCH 96 (1) 12 -17 2005年04月 [査読無し][通常論文]
     
    Immune responses to oral infection with Echinococcus multilocularis protoscoleces in Mongolian gerbils were investigated. Gerbils not treated with prednisolone expelled most of the parasites within 3 days post-infection and induced parasite-specific intestinal IgA secretion after the oral inoculation with protoscoleces. In contrast, prednisolone-treated gerbils harbored notable numbers of parasites, and the parasite-specific intestinal IgA secretion was lower. In gerbils not treated and orally inoculated with protoscoleces (infected group), parasite-specific antibody levels in sera and intestinal washings were elevated, but blastogenesis against protoscolex antigens was observed only in cells from Peyer's patches at 14 days post-infection. Concanavalin A-induced proliferative lymphocytes from both infected and naive gerbils were suppressed by adding protoscolex somatic antigen. These data indicate that while parasite-specific antibody responses were observed, lymphocyte proliferative responses were suppressed by parasite infection/antigens in the Mongolian gerbil, prednisolone-untreated rodent definitive host model.
  • N Kato, N Nonaka, Y Oku, M Kamiya PARASITOLOGY RESEARCH 96 (1) 12 -17 2005年04月 [査読無し][通常論文]
     
    Immune responses to oral infection with Echinococcus multilocularis protoscoleces in Mongolian gerbils were investigated. Gerbils not treated with prednisolone expelled most of the parasites within 3 days post-infection and induced parasite-specific intestinal IgA secretion after the oral inoculation with protoscoleces. In contrast, prednisolone-treated gerbils harbored notable numbers of parasites, and the parasite-specific intestinal IgA secretion was lower. In gerbils not treated and orally inoculated with protoscoleces (infected group), parasite-specific antibody levels in sera and intestinal washings were elevated, but blastogenesis against protoscolex antigens was observed only in cells from Peyer's patches at 14 days post-infection. Concanavalin A-induced proliferative lymphocytes from both infected and naive gerbils were suppressed by adding protoscolex somatic antigen. These data indicate that while parasite-specific antibody responses were observed, lymphocyte proliferative responses were suppressed by parasite infection/antigens in the Mongolian gerbil, prednisolone-untreated rodent definitive host model.
  • N Kato, N Nonaka, Y Oku, M Kamiya PARASITOLOGY RESEARCH 95 (5) 339 -345 2005年03月 [査読無し][通常論文]
     
    Parasite-specific antigen responses and lymphocyte blastogenesis in dogs orally inoculated with Echinococcus multilocuralis metacestodes were examined. Serum IgG1 (Th2-oriented) and IgG2 (Th 1-oriented) levels against somatic and excretory-secretory (ES) antigens of protoscoleces and adult worms increased from 7 days post-infection (DPI), with the highest responses against protoscolex excretory-secretory antigen (PES). Specific blastogenesis of peripheral blood mononuclear cells (PBMC) against the parasite antigens was not observed during the 21-day infection period, but Peyer's patches cells from one out of two dogs at 21 DPI showed blastogenesis against PES (stimulation index: 4.65). Interestingly, only at 7 DPI were concanavalin A (ConA)-induce proliferative responses of PBMC reduced. Moreover, ConA-induced proliferative responses of lymphocytes from various origins were suppressed by the addition of parasite antigens, especially with PES. These data suggest that although both Th1- and Th2-oriented humoral immune responses were observed in E. multilocularis infected dogs, the parasite antigens, especially PES, may have incompletely suppressed lymphocyte responses in these dogs.
  • C Casaravilla, R Malgor, A Rossi, H Sakai, N Nonaka, M Kamiya, C Carmona PARASITOLOGY INTERNATIONAL 54 (1) 43 -49 2005年03月 [査読無し][通常論文]
     
    Two IgM murine monoclonal antibodies (MAbs), EgC1 and EgC3, were produced against the excretory/secretory (E/S) products of Echinococcus granulosus adult worms. Immunoblotting revealed that both predominantly recognized a 50 kDa antigen in the somatic extract and an 85 kDa component in the E/S products. Immumolocalization showed that both MAbs reacted with the tegument of the parasite, and additionally EgC3 reacted with parenchyma and the tegument lining the external surface of the reproductive organs. A coproantigen capture ELISA was developed using a rabbit polyclonal antibody against E/S products from adult tapeworms as catching antibodies, and each one of MAbs as detecting antibody. The assays detected seven out of eight (EgC1), and eight out of eight (EgC3) experimentally infected dogs (worm burdens ranging from 61 to 57,500), using heat-treated samples obtained at prepatent period, and none (n=8) of helminth-free samples. Time course analysis showed that, after a 12-25 days lag, coproantigen levels rose above cut off O.D. values and typically peaked around 30 days post-infection (DPI) at the end of the experiment. One dog experimentally infected with Taenia hydatigena metacestodes was slightly detected as positive at different time points after 30 DPI. Both MAbs showed a similar pattern of recognition, but T hydatigena antigens were undetectable for a longer period, and reached lower O.D. values with EgC1. Interestingly, fecal samples from two experimentally infected dogs with Echinococcus multilocularis were not recognized by the EgC1 assay, suggesting a potential value as species-specific diagnostic tool. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
  • N Kato, N Nonaka, Y Oku, M Kamiya PARASITOLOGY RESEARCH 95 (5) 339 -345 2005年03月 [査読無し][通常論文]
     
    Parasite-specific antigen responses and lymphocyte blastogenesis in dogs orally inoculated with Echinococcus multilocuralis metacestodes were examined. Serum IgG1 (Th2-oriented) and IgG2 (Th 1-oriented) levels against somatic and excretory-secretory (ES) antigens of protoscoleces and adult worms increased from 7 days post-infection (DPI), with the highest responses against protoscolex excretory-secretory antigen (PES). Specific blastogenesis of peripheral blood mononuclear cells (PBMC) against the parasite antigens was not observed during the 21-day infection period, but Peyer's patches cells from one out of two dogs at 21 DPI showed blastogenesis against PES (stimulation index: 4.65). Interestingly, only at 7 DPI were concanavalin A (ConA)-induce proliferative responses of PBMC reduced. Moreover, ConA-induced proliferative responses of lymphocytes from various origins were suppressed by the addition of parasite antigens, especially with PES. These data suggest that although both Th1- and Th2-oriented humoral immune responses were observed in E. multilocularis infected dogs, the parasite antigens, especially PES, may have incompletely suppressed lymphocyte responses in these dogs.
  • C Casaravilla, R Malgor, A Rossi, H Sakai, N Nonaka, M Kamiya, C Carmona PARASITOLOGY INTERNATIONAL 54 (1) 43 -49 2005年03月 [査読無し][通常論文]
     
    Two IgM murine monoclonal antibodies (MAbs), EgC1 and EgC3, were produced against the excretory/secretory (E/S) products of Echinococcus granulosus adult worms. Immunoblotting revealed that both predominantly recognized a 50 kDa antigen in the somatic extract and an 85 kDa component in the E/S products. Immumolocalization showed that both MAbs reacted with the tegument of the parasite, and additionally EgC3 reacted with parenchyma and the tegument lining the external surface of the reproductive organs. A coproantigen capture ELISA was developed using a rabbit polyclonal antibody against E/S products from adult tapeworms as catching antibodies, and each one of MAbs as detecting antibody. The assays detected seven out of eight (EgC1), and eight out of eight (EgC3) experimentally infected dogs (worm burdens ranging from 61 to 57,500), using heat-treated samples obtained at prepatent period, and none (n=8) of helminth-free samples. Time course analysis showed that, after a 12-25 days lag, coproantigen levels rose above cut off O.D. values and typically peaked around 30 days post-infection (DPI) at the end of the experiment. One dog experimentally infected with Taenia hydatigena metacestodes was slightly detected as positive at different time points after 30 DPI. Both MAbs showed a similar pattern of recognition, but T hydatigena antigens were undetectable for a longer period, and reached lower O.D. values with EgC1. Interestingly, fecal samples from two experimentally infected dogs with Echinococcus multilocularis were not recognized by the EgC1 assay, suggesting a potential value as species-specific diagnostic tool. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
  • Identification of taeniid cestode eggs - extraction of egg DNA and use of COI gene
    Japanese Journal of Veterinary Parasitology 3 (2) 31 2005年 [査読無し][通常論文]
  • A servey of gastrointestinal parasites in dogs and cats in Kanto district
    Japanese Journal of Veterinary Parasitology 3 (2) 47 2005年 [査読無し][通常論文]
  • 飼い犬のエキノコックス感染とその診断
    獣医畜産新報 58 (4) 341 -342 2005年 [査読無し][通常論文]
  • Epizootiology of Echinococcus multilocularis in the northeastern region of Sapporo
    Japanese Journal of Veterinary Parasitology 3 (2) 30 2005年 [査読無し][通常論文]
  • Identification of taeniid cestode eggs - extraction of egg DNA and use of COI gene
    Japanese Journal of Veterinary Parasitology 3 (2) 31 2005年 [査読無し][通常論文]
  • A servey of gastrointestinal parasites in dogs and cats in Kanto district
    Japanese Journal of Veterinary Parasitology 3 (2) 47 2005年 [査読無し][通常論文]
  • 飼い犬のエキノコックス感染とその診断
    獣医畜産新報 58 (4) 341 -342 2005年 [査読無し][通常論文]
  • Epizootiology of Echinococcus multilocularis in the northeastern region of Sapporo
    Japanese Journal of Veterinary Parasitology 3 (2) 30 2005年 [査読無し][通常論文]
  • 条虫症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 247-251 2004年 [査読無し][通常論文]
  • 条虫症,主要症状を基礎にした猫の臨床
    デーリィマン,札幌 247-251 2004年 [査読無し][通常論文]
  • Japanese Journal of Public Health 50(7), 639-649 2003年 [査読無し][通常論文]
  • Japanese Journal of Public Health 50(7), 639-649 2003年 [査読無し][通常論文]
  • H Tsukada, K Hamazaki, S Ganzorig, T Iwaki, K Konno, JT Lagapa, K Matsuo, A Ono, M Shimizu, H Sakai, Y Morishima, N Nonaka, Y Oku, M Kamiya PARASITOLOGY 125 119 -129 2002年08月 [査読無し][通常論文]
     
    The effect of bait-delivered anthelmintic to reduce the prevalence of Echinococcus multilocularis in wild red foxes was evaluated in Koshimizu, in the eastern part of Hokkaido, Japan. The study area (200 km(2)) was divided into baited and non-baited sections. The anthelmintic baits were distributed around fox den sites in the baited section every month for 13 months. After 1 year of the anthelmintic bait distribution, the prevalence of E. multilocularis in foxes, evaluated either by the parasite egg examination (from 27.1 to 5.6%) or coproantigen ELISA (from 59.6 to 29.7%), decreased in the baited section contrasting to that in the non-baited section (parasite egg: from 18.8 to 24.2%; ELISA: from 41.9 to 45.8%). The prevalence of E. multilocularis in grey red-backed vole Clethrionomys rufocanus, caught around fox dens, born after bait distribution also decreased and was significantly lower than that in non-baited section. However, within the study periods, the coproantigen-positive rate in fox faeces sporadically increased, while egg-positive rate constantly decreased. Since coproantigen ELISA can detect pre-patent infection, this observation indicates that reinfection pressure in the baited section was still high even after the 13 months of anthelmintic bait distribution. Therefore, the bait distribution longer than our study period is required for the efficient control of E. multilocularis in wild red fox population.
  • H Tsukada, K Hamazaki, S Ganzorig, T Iwaki, K Konno, JT Lagapa, K Matsuo, A Ono, M Shimizu, H Sakai, Y Morishima, N Nonaka, Y Oku, M Kamiya PARASITOLOGY 125 119 -129 2002年08月 [査読無し][通常論文]
     
    The effect of bait-delivered anthelmintic to reduce the prevalence of Echinococcus multilocularis in wild red foxes was evaluated in Koshimizu, in the eastern part of Hokkaido, Japan. The study area (200 km(2)) was divided into baited and non-baited sections. The anthelmintic baits were distributed around fox den sites in the baited section every month for 13 months. After 1 year of the anthelmintic bait distribution, the prevalence of E. multilocularis in foxes, evaluated either by the parasite egg examination (from 27.1 to 5.6%) or coproantigen ELISA (from 59.6 to 29.7%), decreased in the baited section contrasting to that in the non-baited section (parasite egg: from 18.8 to 24.2%; ELISA: from 41.9 to 45.8%). The prevalence of E. multilocularis in grey red-backed vole Clethrionomys rufocanus, caught around fox dens, born after bait distribution also decreased and was significantly lower than that in non-baited section. However, within the study periods, the coproantigen-positive rate in fox faeces sporadically increased, while egg-positive rate constantly decreased. Since coproantigen ELISA can detect pre-patent infection, this observation indicates that reinfection pressure in the baited section was still high even after the 13 months of anthelmintic bait distribution. Therefore, the bait distribution longer than our study period is required for the efficient control of E. multilocularis in wild red fox population.
  • Parasitology International 51(1), 81-89 2002年 [査読無し][通常論文]
  • Prevalence and intensity of Echinococcus multilocularis in red foxes (Vulpes vulpes schrencki) and raccoon dogs (Nyctereutes procyonoides albus) in Otaru city, Hokkaido, Japan.
    Japanese Journal of Veterinary Research 49(4), 287-296 2002年 [査読無し][通常論文]
  • Japanese Journal of Veterinary Research 49(4), 273-285 2002年 [査読無し][通常論文]
  • Parasitology International 51(1), 81-89 2002年 [査読無し][通常論文]
  • Prevalence and intensity of Echinococcus multilocularis in red foxes (Vulpes vulpes schrencki) and raccoon dogs (Nyctereutes procyonoides albus) in Otaru city, Hokkaido, Japan.
    Japanese Journal of Veterinary Research 49(4), 287-296 2002年 [査読無し][通常論文]
  • Japanese Journal of Veterinary Research 49(4), 273-285 2002年 [査読無し][通常論文]
  • AE Yimam, Y Oku, N Nonaka, H Sakai, Y Morishima, K Matsuo, G La Rosa, E Pozio, K Yagi, M Kamiya PARASITOLOGY INTERNATIONAL 50 (2) 121 -127 2001年07月 [査読無し][通常論文]
     
    Forty-three red foxes (Vulpes vulpes schrencki) and nine raccoon dogs (Nyctereutes procyonoides viverrinus) were captured in Otaru City, Hokkaido, Japan and examined by muscle digestion for the presence of Trichinella sp. larvae. Of the foxes, five (11.6%) were positive for larvae of Trichinella nativa while none of the raccoon dogs were found to be positive. This finding suggests that the red foxes are important reservoir hosts of sylvatic trichinellosis in Otaru, Hokkaido. This is the first report on Trichinella nativa in wild red foxes of Hokkaido, Japan. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
  • AE Yimam, Y Oku, N Nonaka, H Sakai, Y Morishima, K Matsuo, G La Rosa, E Pozio, K Yagi, M Kamiya PARASITOLOGY INTERNATIONAL 50 (2) 121 -127 2001年07月 [査読無し][通常論文]
     
    Forty-three red foxes (Vulpes vulpes schrencki) and nine raccoon dogs (Nyctereutes procyonoides viverrinus) were captured in Otaru City, Hokkaido, Japan and examined by muscle digestion for the presence of Trichinella sp. larvae. Of the foxes, five (11.6%) were positive for larvae of Trichinella nativa while none of the raccoon dogs were found to be positive. This finding suggests that the red foxes are important reservoir hosts of sylvatic trichinellosis in Otaru, Hokkaido. This is the first report on Trichinella nativa in wild red foxes of Hokkaido, Japan. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
  • H Sakai, JV Barbosa, EM Silva, FO Schlabitz, RP Noronha, N Nonaka, CR Franke, H Ueno AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE 64 (5-6) 268 -269 2001年05月 [査読無し][通常論文]
     
    To determine the prevalence and etiologic factors of Taenia solium cysticercosis in pigs in the state of Bahia, northeastern Brazil, we performed serological surveys in Salvador, the capital of the state, and in two local towns, Santo Amaro and Jequie. Residents raising free-roaming pigs in the study areas were randomly selected. Sera were obtained from the pigs and examined by the enzyme-linked immunoelectrotransfer blot assay (EITB). The prevalence of antibodies to T. solium was 4.4% (2 of 45) in Salvador, 3.2% (3 of 93) in Santo Amaro, and 23.5% (24 of 102) in Jequie. A significantly high seroprevalence in Jequie was associated with poor sanitary conditions, such as an open sewer system and no inspection process of pork before marketing.
  • H Sakai, JV Barbosa, EM Silva, FO Schlabitz, RP Noronha, N Nonaka, CR Franke, H Ueno AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE 64 (5-6) 268 -269 2001年05月 [査読無し][通常論文]
     
    To determine the prevalence and etiologic factors of Taenia solium cysticercosis in pigs in the state of Bahia, northeastern Brazil, we performed serological surveys in Salvador, the capital of the state, and in two local towns, Santo Amaro and Jequie. Residents raising free-roaming pigs in the study areas were randomly selected. Sera were obtained from the pigs and examined by the enzyme-linked immunoelectrotransfer blot assay (EITB). The prevalence of antibodies to T. solium was 4.4% (2 of 45) in Salvador, 3.2% (3 of 93) in Santo Amaro, and 23.5% (24 of 102) in Jequie. A significantly high seroprevalence in Jequie was associated with poor sanitary conditions, such as an open sewer system and no inspection process of pork before marketing.
  • A survey of canine echinococcosis in Gobi Altai Province of Mongolia by coproantigen detection.
    Japanese Journal of Veterinary Research 49(2), 125-129 2001年 [査読無し][通常論文]
  • Parasitology Research 87(8), 593-597 2001年 [査読無し][通常論文]
  • A survey of canine echinococcosis in Gobi Altai Province of Mongolia by coproantigen detection.
    Japanese Journal of Veterinary Research 49(2), 125-129 2001年 [査読無し][通常論文]
  • Parasitology Research 87(8), 593-597 2001年 [査読無し][通常論文]
  • K Matsuo, N Nonaka, Y Oku, M Kamiya PARASITOLOGY RESEARCH 86 (6) 521 -523 2000年06月 [査読無し][通常論文]
     
    Mongolian gerbils (Meriones unguiculatus) were orally inoculated with 10,000 protoscoleces of Echinococcus multilocularis per head after being divided into five groups (A-E). Each group was dosed with prednisolone tertiary-butylacetate (PTBA) as follows: A, 0 mg; B, 0.5 mg; C, 2 mg; D, 5 mg; and E, 10 mg/head. All animals were injected subcutaneously with control solvent or PTBA every other day from 6 days pre- to 6 days post-infection. Autopsy was performed at 7 days post-infection. Doses of PTBA and the number of worms recovered showed a positive correlation (r = 0.929, P < 0.0001). In groups A, B and C, the predilection site of the worms in the small intestine could not be determined, while in group D the worms were found more in the anterior part. In group E, the predilection site was the anterior part, followed by the middle and the posterior parts of the small intestine (Fisher's test: P < 0.01). The number of worms recovered from the anterior and the middle part of the small intestine also correlated positively with PTBA dose: (anterior part: v = 0.930, P < 0.0001, middle part: v = 0.917, P < 0.0001). All groups of the PTBA-treated animals showed significant loss of weight compared to the nontreated animals (P < 0.01).
  • K Matsuo, N Nonaka, Y Oku, M Kamiya PARASITOLOGY RESEARCH 86 (6) 521 -523 2000年06月 [査読無し][通常論文]
     
    Mongolian gerbils (Meriones unguiculatus) were orally inoculated with 10,000 protoscoleces of Echinococcus multilocularis per head after being divided into five groups (A-E). Each group was dosed with prednisolone tertiary-butylacetate (PTBA) as follows: A, 0 mg; B, 0.5 mg; C, 2 mg; D, 5 mg; and E, 10 mg/head. All animals were injected subcutaneously with control solvent or PTBA every other day from 6 days pre- to 6 days post-infection. Autopsy was performed at 7 days post-infection. Doses of PTBA and the number of worms recovered showed a positive correlation (r = 0.929, P < 0.0001). In groups A, B and C, the predilection site of the worms in the small intestine could not be determined, while in group D the worms were found more in the anterior part. In group E, the predilection site was the anterior part, followed by the middle and the posterior parts of the small intestine (Fisher's test: P < 0.01). The number of worms recovered from the anterior and the middle part of the small intestine also correlated positively with PTBA dose: (anterior part: v = 0.930, P < 0.0001, middle part: v = 0.917, P < 0.0001). All groups of the PTBA-treated animals showed significant loss of weight compared to the nontreated animals (P < 0.01).
  • K Matsuo, M Shimizu, N Nonaka, Y Oku, M Kamiya ACTA TROPICA 75 (3) 323 -330 2000年05月 [査読無し][通常論文]
     
    An alternative definitive host model for Echinococcus vogeli was developed using Mongolian gerbils. The animals treated with prednisolone tert-butylacetate were orally inoculated with protoscoleces of E. vogeli and the development of worms in the Small intestine was observed until 50 days post infection (DPI). The parasites were recovered most frequently from the anterior part of the small intestine. The band formation (the sign of proglottisation) of the worm was observed at 7 DPI, the second proglottid and tests formation at 14 DPI, spermatozoa in seminal receptacle and cleavage of ova in uterus at 21 DPI, the hook formation of oncospheres at 28 DPI and the embryophore formation at 34 DPI. Eggs in the feces were first detected at 35 DPI. The coproantigen detection method developed for E. multilocularis could detect the E. vogeli coproantigen early in the infection, at 7 DPI during the prepatent period. Therefore, this technique could be used for the diagnosis of E. vogeli, replacing ordinary fecal egg examination. (C) 2000 Elsevier Science B.V. All rights reserved.
  • K Matsuo, M Shimizu, N Nonaka, Y Oku, M Kamiya ACTA TROPICA 75 (3) 323 -330 2000年05月 [査読無し][通常論文]
     
    An alternative definitive host model for Echinococcus vogeli was developed using Mongolian gerbils. The animals treated with prednisolone tert-butylacetate were orally inoculated with protoscoleces of E. vogeli and the development of worms in the Small intestine was observed until 50 days post infection (DPI). The parasites were recovered most frequently from the anterior part of the small intestine. The band formation (the sign of proglottisation) of the worm was observed at 7 DPI, the second proglottid and tests formation at 14 DPI, spermatozoa in seminal receptacle and cleavage of ova in uterus at 21 DPI, the hook formation of oncospheres at 28 DPI and the embryophore formation at 34 DPI. Eggs in the feces were first detected at 35 DPI. The coproantigen detection method developed for E. multilocularis could detect the E. vogeli coproantigen early in the infection, at 7 DPI during the prepatent period. Therefore, this technique could be used for the diagnosis of E. vogeli, replacing ordinary fecal egg examination. (C) 2000 Elsevier Science B.V. All rights reserved.
  • H Tsukada, Y Morishima, N Nonaka, Y Oku, M Kamiya PARASITOLOGY 120 (4) 423 -428 2000年04月 [査読無し][通常論文]
     
    In order to assess the infection risk of alveolar echinococcosis among urban residents of Sapporo, the capital of Hokkaido, Japan, a survey was conducted on fox distribution in the urban area and on the prevalence of Echinococcus multilocularis among the foxes. The fox distribution, evaluated from fox footprints left on the snow in parks and woodlands, and from locations of fox carcasses recorded by the Sapporo municipality, was concentrated along the border of the urban area and in the southwestern part of the city, facing the mountain. Fox faeces were collected around active fox dens, and analysed by a coproantigen detection assay and parasite egg examination for the Echinococcus infection. Thirty-three out of 155 faeces were coproantigen positive. Coproantigen-positive faeces were collected from 11 den sites (57.9 %, of total den sites), and all except 1 were located in the urban fringe. A high intensity of taeniid eggs (> 100 eggs per 0.5 g) containing faeces were also collected in the 3 sites of them. Although Echinococcus infection in rodents was not observed from the necropsy of 23 rodents captured around active fox dens, arvicolid rodents, a suitable intermediate host for E. multilocularis, were captured in the urban fringe. Therefore, the urban fringe offers suitable conditions in which the life-cycle of E. multilocularis could be maintained. Prompt measures to control echinococcus infection should be taken, even in urban areas.
  • K Matsuo, T Tada, N Nonaka, Y Oku, M Kamiya JAPANESE JOURNAL OF VETERINARY RESEARCH 47 (3-4) 145 -149 2000年02月 [査読無し][通常論文]
     
    This study revealed the dose dependency of prednisolone tertiary-butylacetate (PTBA) treatment on the establishment of Echinococcus multilocularis in the small intestine of Mongolian gerbil (Meriones unguiculatus) and that some of the physiological parameters of host were correlated with the doses of PTBA and establishment of the worm. Twenty Mongolian gerbils were divided into 5 groups, according to the doses of PTBA; 0 mg, 0.5 mg, 2 mg, 5 mg and 10 mg per head. All animals were injected intraperitoneally with PTBA every other day from 6 days before to 6 days after infection. Doses of PTBA and the number of worms recovered at 7 days postinfection showed a positive correlation (r= 0.929, P < 0.0001). The increase of total protein (TP) and the decrease of the percentage of lymphocytes in the peripheral leukocytes were dependent on doses of PTBA (TP : r= 0.811, P < 0.0001, percentage of lymphocyte: r= -0.92, P < 0.0001). The TP and the percentage of lymphocyte also correlated with the number of worms recovered (TP: r= 0.617, P = 0.0049; percentage of lymphocyte: r= -0.800, P < 0.0001).
  • K Matsuo, T Tada, N Nonaka, Y Oku, M Kamiya JAPANESE JOURNAL OF VETERINARY RESEARCH 47 (3-4) 145 -149 2000年02月 [査読無し][通常論文]
     
    This study revealed the dose dependency of prednisolone tertiary-butylacetate (PTBA) treatment on the establishment of Echinococcus multilocularis in the small intestine of Mongolian gerbil (Meriones unguiculatus) and that some of the physiological parameters of host were correlated with the doses of PTBA and establishment of the worm. Twenty Mongolian gerbils were divided into 5 groups, according to the doses of PTBA; 0 mg, 0.5 mg, 2 mg, 5 mg and 10 mg per head. All animals were injected intraperitoneally with PTBA every other day from 6 days before to 6 days after infection. Doses of PTBA and the number of worms recovered at 7 days postinfection showed a positive correlation (r= 0.929, P < 0.0001). The increase of total protein (TP) and the decrease of the percentage of lymphocytes in the peripheral leukocytes were dependent on doses of PTBA (TP : r= 0.811, P < 0.0001, percentage of lymphocyte: r= -0.92, P < 0.0001). The TP and the percentage of lymphocyte also correlated with the number of worms recovered (TP: r= 0.617, P = 0.0049; percentage of lymphocyte: r= -0.800, P < 0.0001).
  • H. Tsukada, Y. Morishima, N. Nonaka, Y. Oku, M. Kamiya Parasitology 120 (4) 423 -428 2000年 [査読無し][通常論文]
     
    In order to assess the infection risk of alveolar echinococcosis among urban residents of Sapporo, the capital of Hokkaido, Japan, a survey was conducted on fox distribution in the urban area and on the prevalence of Echinococcus multilocularis among the foxes. The fox distribution, evaluated from fox footprints left on the snow in parks and woodlands, and from locations of fox carcasses recorded by the Sapporo municipality, was concentrated along the border of the urban area and in the southwestern part of the city, facing the mountain. Fox faeces were collected around active fox dens, and analysed by a coproantigen detection assay and parasite egg examination for the Echinococcus infection. Thirty-three out of 155 faeces were coproantigen positive. Coproantigen-positive faeces were collected from 11 den sites (57.9% of total den sites), and all except 1 were located in the urban fringe. A high intensity of taeniid eggs (> 100 eggs per 0.5 g) containing faeces were also collected in the 3 sites of them. Although Echinococcus infection in rodents was not observed from the necropsy of 23 rodents captured around fox dens, arvicolid rodents, a suitable intermediate host for E. multilocularis, were captured in the urban fringe. Therefore, the urban fringe offers suitable conditions in which the life-cycle of E. multilocularis could be maintained. Prompt measures to control echinococcus infection should be taken, even in urban areas.
  • Yasuyuki Morishima, Hideharu Tsukada, Nariaki Nonaka, Yuzaburo Oku, Masao Kamiya Parasitology International 48 (2) 121 -134 1999年08月01日 [査読無し][通常論文]
     
    An epidemiological survey was conducted on the seasonal variation of Echinococcus multilocularis prevalence in red foxes from 1997 to 1998, using a monoclonal antibody-based detection of the tapeworm coproantigen. Thirty-six breeding dens of reproductive fox families were identified in the endemic area of Koshimizu, eastern Hokkaido, Japan. Fecal samples from each site were examined by coproantigen detection assay and fecal egg examination. Whereas the prevalence of coproantigen positive feces showed no seasonal fluctuation (51.6-66.7%), variation was found in the prevalence of egg positive feces in which a higher prevalence was observed in the summer and winter (31.1 and 38.7%) than spring and autumn (13.3 and 13.5%). Significant differences were observed between juveniles and adult foxes in both examinations. Samples from juvenile foxes gave higher coproantigen positive results and taeniid egg intensity. Those results suggest more juveniles infected with the cestode than adults in the same period. The practical use of coproantigen assay as a survey tool and factors which affect the prevalence and host age-related difference are discussed. Copyright (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
  • Yasuyuki Morishima, Hideharu Tsukada, Nariaki Nonaka, Yuzaburo Oku, Masao Kamiya Parasitology International 48 (2) 121 -134 1999年08月01日 [査読無し][通常論文]
     
    An epidemiological survey was conducted on the seasonal variation of Echinococcus multilocularis prevalence in red foxes from 1997 to 1998, using a monoclonal antibody-based detection of the tapeworm coproantigen. Thirty-six breeding dens of reproductive fox families were identified in the endemic area of Koshimizu, eastern Hokkaido, Japan. Fecal samples from each site were examined by coproantigen detection assay and fecal egg examination. Whereas the prevalence of coproantigen positive feces showed no seasonal fluctuation (51.6-66.7%), variation was found in the prevalence of egg positive feces in which a higher prevalence was observed in the summer and winter (31.1 and 38.7%) than spring and autumn (13.3 and 13.5%). Significant differences were observed between juveniles and adult foxes in both examinations. Samples from juvenile foxes gave higher coproantigen positive results and taeniid egg intensity. Those results suggest more juveniles infected with the cestode than adults in the same period. The practical use of coproantigen assay as a survey tool and factors which affect the prevalence and host age-related difference are discussed. Copyright (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
  • K Konno, Y Oku, N Nonaka, M Kamiya PARASITOLOGY RESEARCH 85 (6) 431 -436 1999年06月 [査読無し][通常論文]
     
    Rats heavily infected with Taenia taeniaeformis larvae in the liver show a remarkable increase in their stomach weight, hyperplasia, and hypergastrinemia. However, it is unknown what causes these phenomena. Hence, as a preliminary study to investigate the importance of larval parasitism in the liver, two experiments were done. In the first experiment, 14 donor rats were orally inoculated with 3,000 T. taeniaeformis eggs. In the second experiment, 136-300 of the larvae obtained from the rats were surgically implanted into the abdominal cavity of 7 recipient rats. Gastrin levels and histopathological changes in the gastric mucosa were investigated. In all, II donor rats showed hypergastrinemia and hyperplasia, 5 recipient rats showed gastric mucosal hyperplasia accompanied by excessive mucous cell proliferation, and 2 recipient rats showed hypergastrinemia. These results suggest that parasitism of the liver by the larvae is not essential for the development of hyperplasia and that factors from the larvae might cause these phenomena.
  • Kenjiro Konno, Yuzaburo Oku, Nariaki Nonaka, Masao Kamiya Parasitology Research 85 (6) 431 -436 1999年06月 [査読無し][通常論文]
     
    Rats heavily infected with Taenia taeniaeformis larvae in the liver show a remarkable increase in their stomach weight, hyperplasia, and hypergastrinemia. However, it is unknown what causes these phenomena. Hence, as a preliminary study to investigate the importance of larval parasitism in the liver, two experiments were done. In the first experiment, 14 donor rats were orally inoculated with 3,000 T. taeniaeformis eggs. In the second experiment, 136-300 of the larvae obtained from the rats were surgically implanted into the abdominal cavity of 7 recipient rats. Gastrin levels and histopathological changes in the gastric mucosa were investigated. In all, 11 donor rats showed hypergastrinemia and hyperplasia, 5 recipient rats showed gastric mucosal hyperplasia accompanied by excessive mucous cell proliferation, and 2 recipient rats showed hypergastrinemia. These results suggest that parasitism of the liver by the larvae is not essential for the development of hyperplasia and that factors from the larvae might cause these phenomena.
  • K Konno, JA Abella, Y Oku, N Nonaka, M Kamiya JOURNAL OF VETERINARY MEDICAL SCIENCE 61 (4) 317 -324 1999年04月 [査読無し][通常論文]
     
    Rats heavily infected with larva! Taenia taeniaeformis show hyperplasia of the gastric mucosa accompanied by mucous cell proliferation, increase in the level of intragastric pH and hypergastrinemia. Sixty one rats were divided into 2 groups designed as infected (36 rats) and control (25 rats) group. These rats were examined with time course of the infection histopathologically and physiopathologically, during 14-112 days postinfection (DPI). In the infected rats, gastric mucosal hyperplasia began to be observed at 56 DPI, and the structural disturbance of zymogenic units in the corpus and mucous units in the antrum had increased with time. However, the degree of these changes in the antrum was weaker than those in the corpus. Alcianblue and/or PAS-positive cells increased in their numbers with time, and 4 types of cells other than typical surface mucous cell and mucous neck cell were observed by electronmicroscopy. However, zymogenic and parietal cells decreased in their number after 56 DPI. Further, the infected rats showed changes in the serum concentration of alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, glucose and total protein. Some similarities with Menetrier's disease were discussed.
  • K Konno, JA Abella, Y Oku, N Nonaka, M Kamiya JOURNAL OF VETERINARY MEDICAL SCIENCE 61 (4) 317 -324 1999年04月 [査読無し][通常論文]
     
    Rats heavily infected with larva! Taenia taeniaeformis show hyperplasia of the gastric mucosa accompanied by mucous cell proliferation, increase in the level of intragastric pH and hypergastrinemia. Sixty one rats were divided into 2 groups designed as infected (36 rats) and control (25 rats) group. These rats were examined with time course of the infection histopathologically and physiopathologically, during 14-112 days postinfection (DPI). In the infected rats, gastric mucosal hyperplasia began to be observed at 56 DPI, and the structural disturbance of zymogenic units in the corpus and mucous units in the antrum had increased with time. However, the degree of these changes in the antrum was weaker than those in the corpus. Alcianblue and/or PAS-positive cells increased in their numbers with time, and 4 types of cells other than typical surface mucous cell and mucous neck cell were observed by electronmicroscopy. However, zymogenic and parietal cells decreased in their number after 56 DPI. Further, the infected rats showed changes in the serum concentration of alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, glucose and total protein. Some similarities with Menetrier's disease were discussed.
  • Y Morishima, H Tsukada, N Nonaka, Y Oku, M Kamiya JAPANESE JOURNAL OF VETERINARY RESEARCH 46 (4) 185 -189 1999年02月 [査読無し][通常論文]
     
    The validity of a coproantigen ELISA for Echinococcus multilocularis was evaluated by comparison of three diagnostic methods; autopsy, egg examination and the ELISA. Of 71 foxes, 39 were found to be infected with the cestode at autopsy. The overall mean of worm burdens was 3,451, but the number varied (1-34, 522). The ELISA could detect 94.9% (37/39) of the worm positives and there were no false-positives. Two false-negatives were infected with 1 and 4 cestodes, whereas 3 cases with similar worm burdens (2, 4 and 6 worms) were diagnosed as positives. This indicates the detection limit of the assay may be equivalent to less than 10 (in the worm burden). On the other hand, egg examination showed low sensitivity (43.6%, 17/39). These results suggest the ELISA has a potential to replace for the conventional methods.
  • Y Morishima, H Tsukada, N Nonaka, Y Oku, M Kamiya JAPANESE JOURNAL OF VETERINARY RESEARCH 46 (4) 185 -189 1999年02月 [査読無し][通常論文]
     
    The validity of a coproantigen ELISA for Echinococcus multilocularis was evaluated by comparison of three diagnostic methods; autopsy, egg examination and the ELISA. Of 71 foxes, 39 were found to be infected with the cestode at autopsy. The overall mean of worm burdens was 3,451, but the number varied (1-34, 522). The ELISA could detect 94.9% (37/39) of the worm positives and there were no false-positives. Two false-negatives were infected with 1 and 4 cestodes, whereas 3 cases with similar worm burdens (2, 4 and 6 worms) were diagnosed as positives. This indicates the detection limit of the assay may be equivalent to less than 10 (in the worm burden). On the other hand, egg examination showed low sensitivity (43.6%, 17/39). These results suggest the ELISA has a potential to replace for the conventional methods.
  • The spreading of sarcoptic mange among red foxes Vulpes vulpes and its impact on the local fox population in Shiretoko Peninsula, Hokkaido, Japan.
    Mammalian Science 39 (2) 247 -256 1999年 [査読無し][通常論文]
  • Taenia crassiceps larvae in chinchilla, Chinchilla brevicaudata.
    Journal of Veterinary Medicine(Tokyo) 52 449 -452 1999年 [査読無し][通常論文]
  • The spreading of sarcoptic mange among red foxes Vulpes vulpes and its impact on the local fox population in Shiretoko Peninsula, Hokkaido, Japan.
    Mammalian Science 39 (2) 247 -256 1999年 [査読無し][通常論文]
  • Taenia crassiceps larvae in chinchilla, Chinchilla brevicaudata.
    Journal of Veterinary Medicine(Tokyo) 52 449 -452 1999年 [査読無し][通常論文]
  • H Sakai, M Sone, DM Castro, N Nonaka, D Quan, M Canales, Ljungstrom, I, AL Sanchez VETERINARY PARASITOLOGY 78 (3) 233 -238 1998年08月 [査読無し][通常論文]
     
    Several retrospective studies have shown that human Taenia solium cysticercosis is endemic in Honduras, but very few reports of porcine cysticercosis in rural communities have been published. To determine the local prevalence of this disease in pigs, a serological survey has been undertaken in a rural community, Salama, in the Department of Olancho in central Honduras. Eighty-five families raising pigs in the community were randomly selected and sera were obtained from pigs older than one month of age. The sera were examined by the enzyme-linked immunoelectrotransfer blot assay (EITB). Of 192 porcine sera, 27.1% (52) were positive by the EITB, Seropositivity did not correlate with age and sex by statistical analysis. With respect to the number and the frequency of recognition of the seven diagnostic glycoprotein bands in the EITB, 67.3% of the positive serum specimens recognized only one band and 80.8% of them recognized GP42-39. Since recognition of GP42-39 has been reported as a characteristic of late infection, these results suggest that most of the seropositive pigs were in the late stage of infection (more than 5-8 weeks postinfection). It seems that pigs in this community may be infected with this parasite soon after the birth and be in a hyperendemic steady state. In view of a high prevalence of antibodies to T. solium in pigs or characteristics of the antigen detected by the EITB, the infection pressure of T. solium appears to be very high in this community. This is probably the case in most of the communities in Honduras. (C) 1998 Elsevier Science B.V. All rights reserved.
  • H Sakai, M Sone, DM Castro, N Nonaka, D Quan, M Canales, Ljungstrom, I, AL Sanchez VETERINARY PARASITOLOGY 78 (3) 233 -238 1998年08月 [査読無し][通常論文]
     
    Several retrospective studies have shown that human Taenia solium cysticercosis is endemic in Honduras, but very few reports of porcine cysticercosis in rural communities have been published. To determine the local prevalence of this disease in pigs, a serological survey has been undertaken in a rural community, Salama, in the Department of Olancho in central Honduras. Eighty-five families raising pigs in the community were randomly selected and sera were obtained from pigs older than one month of age. The sera were examined by the enzyme-linked immunoelectrotransfer blot assay (EITB). Of 192 porcine sera, 27.1% (52) were positive by the EITB, Seropositivity did not correlate with age and sex by statistical analysis. With respect to the number and the frequency of recognition of the seven diagnostic glycoprotein bands in the EITB, 67.3% of the positive serum specimens recognized only one band and 80.8% of them recognized GP42-39. Since recognition of GP42-39 has been reported as a characteristic of late infection, these results suggest that most of the seropositive pigs were in the late stage of infection (more than 5-8 weeks postinfection). It seems that pigs in this community may be infected with this parasite soon after the birth and be in a hyperendemic steady state. In view of a high prevalence of antibodies to T. solium in pigs or characteristics of the antigen detected by the EITB, the infection pressure of T. solium appears to be very high in this community. This is probably the case in most of the communities in Honduras. (C) 1998 Elsevier Science B.V. All rights reserved.
  • J Matsumoto, K Yagi, N Nonaka, Y Oku, M Kamiya PARASITOLOGY 116 463 -469 1998年05月 [査読無し][通常論文]
     
    The kinetics of serum antibody response against infection with Echinococcus multilocularis eggs was evaluated in AKR mice. The animals were infected by oral inoculation with 300 parasite eggs, and necropsied at 1, 2, 4, 6, 9, 12 and 16 weeks post-infection (p.i.), respectively. The parasite formed the laminated layer at 4 weeks p.i., the brood capsule with a massive proliferation of germinal cells at 9 weeks p.i. and protoscoleces at 16 weeks p.i. Serum antibody responses of the mice to antigen preparations from metacestodes of different stages and protoscoleces were evaluated by ELISA, immunoblotting and immunohistochemistry. In ELISA, the antibody responses began to increase at 4 weeks and became more apparent at 9 weeks p.i. and thereafter. Immunoblots using sera collected at 16 weeks p.i. showed some common bands among the 3 different antigen preparations. In addition to this, the germinal cells and brood capsules of mature metacestodes were stained strongly in an immunohistochemical study. From above, it is suggested that some antigen molecules are expressed in the parasite through these stages and stimulated host antibody responses.
  • H Sakai, N Nonaka, K Yagi, Y Oku, M Kamiya JOURNAL OF VETERINARY MEDICAL SCIENCE 60 (5) 639 -641 1998年05月 [査読無し][通常論文]
     
    Detection of Echinococcus coproantigen using sandwich enzyme-linked immunosorbent assay (sELISA) was performed on fecal samples of red foxes in Hokkaido, Japan. Fecal samples were collected around fox dens in 1990 and 1992. The antibodies used for sELISA recognize heat-resistant antigens, thus all fecal samples were heated to render it safe for handling before examination. Detection of taeniid egg in fox feces collected was considered as an indication of E. multilocularis infection. In fecal samples collected in 1990 and 1992, coproantigen positive results out of taeniid-egg positive cases were 38/40 (95.0%) and 95/97 (97.9%), respectively. In addition, coproantigen was detected regardless of fecal condition when collected from the field, suggesting that the antigens detected by this method are quite stable. These results suggest that detection of coproantigen is useful for field surveys of foxes naturally infected with E. multilocularis.
  • J Matsumoto, K Yagi, N Nonaka, Y Oku, M Kamiya PARASITOLOGY 116 463 -469 1998年05月 [査読無し][通常論文]
     
    The kinetics of serum antibody response against infection with Echinococcus multilocularis eggs was evaluated in AKR mice. The animals were infected by oral inoculation with 300 parasite eggs, and necropsied at 1, 2, 4, 6, 9, 12 and 16 weeks post-infection (p.i.), respectively. The parasite formed the laminated layer at 4 weeks p.i., the brood capsule with a massive proliferation of germinal cells at 9 weeks p.i. and protoscoleces at 16 weeks p.i. Serum antibody responses of the mice to antigen preparations from metacestodes of different stages and protoscoleces were evaluated by ELISA, immunoblotting and immunohistochemistry. In ELISA, the antibody responses began to increase at 4 weeks and became more apparent at 9 weeks p.i. and thereafter. Immunoblots using sera collected at 16 weeks p.i. showed some common bands among the 3 different antigen preparations. In addition to this, the germinal cells and brood capsules of mature metacestodes were stained strongly in an immunohistochemical study. From above, it is suggested that some antigen molecules are expressed in the parasite through these stages and stimulated host antibody responses.
  • H Sakai, N Nonaka, K Yagi, Y Oku, M Kamiya JOURNAL OF VETERINARY MEDICAL SCIENCE 60 (5) 639 -641 1998年05月 [査読無し][通常論文]
     
    Detection of Echinococcus coproantigen using sandwich enzyme-linked immunosorbent assay (sELISA) was performed on fecal samples of red foxes in Hokkaido, Japan. Fecal samples were collected around fox dens in 1990 and 1992. The antibodies used for sELISA recognize heat-resistant antigens, thus all fecal samples were heated to render it safe for handling before examination. Detection of taeniid egg in fox feces collected was considered as an indication of E. multilocularis infection. In fecal samples collected in 1990 and 1992, coproantigen positive results out of taeniid-egg positive cases were 38/40 (95.0%) and 95/97 (97.9%), respectively. In addition, coproantigen was detected regardless of fecal condition when collected from the field, suggesting that the antigens detected by this method are quite stable. These results suggest that detection of coproantigen is useful for field surveys of foxes naturally infected with E. multilocularis.
  • Hirofumi Sakai, Nariaki Nonaka, Kinpei Yagi, Yuzaburo Oku, Masao Kamiya Parasitology International 47 (1) 47 -51 1998年03月01日 [査読無し][通常論文]
     
    Detection of Echinococcus multilocularis coproantigen using sandwich enzyme-linked immunosorbent assay (sELISA) was performed on fecal samples of red foxes. Fetal samples were obtained from the rectum of shot foxes. The antibodies used for sELISA recognize heat-resistant antigens, thus all fecal samples were heated to render it safe for handling before examination. E. multilocularis infection in shot foxes were evaluated by the detection of adult worms in the distal one-sixth portion of the small intestine. Among 103 positive cases out of 430 foxes in a necropsy in 1993, 90 samples were also coproantigen positive (the sensitivity is 87.4%). In 11 out of 13 coproantigen negative cases, less than 10 worms were detected at necropsy. These results suggest that detection of coproantigen is useful for field surveys of foxes naturally infected with E. multilocularis.
  • Hirofumi Sakai, Nariaki Nonaka, Kinpei Yagi, Yuzaburo Oku, Masao Kamiya Parasitology International 47 (1) 47 -51 1998年03月01日 [査読無し][通常論文]
     
    Detection of Echinococcus multilocularis coproantigen using sandwich enzyme-linked immunosorbent assay (sELISA) was performed on fecal samples of red foxes. Fetal samples were obtained from the rectum of shot foxes. The antibodies used for sELISA recognize heat-resistant antigens, thus all fecal samples were heated to render it safe for handling before examination. E. multilocularis infection in shot foxes were evaluated by the detection of adult worms in the distal one-sixth portion of the small intestine. Among 103 positive cases out of 430 foxes in a necropsy in 1993, 90 samples were also coproantigen positive (the sensitivity is 87.4%). In 11 out of 13 coproantigen negative cases, less than 10 worms were detected at necropsy. These results suggest that detection of coproantigen is useful for field surveys of foxes naturally infected with E. multilocularis.
  • R Malgor, N Nonaka, Basmadjian, I, H Sakai, B Carambula, Y Oku, C Carmona, M Kamiya INTERNATIONAL JOURNAL FOR PARASITOLOGY 27 (12) 1605 -1612 1997年12月 [査読無し][通常論文]
     
    A sandwich ELISA for the detection of Echinococcus granulosus coproantigen in formalin and heat-treated faecal supernatants of dogs was developed. The assay used affinity-purified polyclonal antibodies obtained from rabbits hyperimmunised with E. granulosus excretory/secretory antigens and biotinylated monoclonal antibody EmA9 produced against adult E. multilocularis somatic extract. The test was sensitive to 7 ng and 2.3 ng of E. granulosus protein and carbohydrate/ml of faecal supernatant, respectively. Thirteen helminth-free dogs were infected with different amounts of E. granulosus protoscoleces and the presence of coproantigen was monitored during the prepatent period until day 35 post-infection, when they were necropsied. Faecal antigen levels started to rise above the normal range between days 10 and 20 post-infection, and typically peaked at the end of the experiment. All the dogs, bearing from 3 to 67 700 worms, showed positive values in the ELISA during the prepatent period. One dog experimentally infected with Taenia hydatigena metacestode and harbouring three worms, tested positive only after the prepatent period at day 52. The test was applied to 98 stray dogs. The ELISA detected all of four dogs naturally infected with E. granulosus, two dogs with patent infections of T. hydatigena and two dogs with no cestode infections, showing a sensitivity of 100% and a specificity of 96%. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.
  • R Malgor, N Nonaka, Basmadjian, I, H Sakai, B Carambula, Y Oku, C Carmona, M Kamiya INTERNATIONAL JOURNAL FOR PARASITOLOGY 27 (12) 1605 -1612 1997年12月 [査読無し][通常論文]
     
    A sandwich ELISA for the detection of Echinococcus granulosus coproantigen in formalin and heat-treated faecal supernatants of dogs was developed. The assay used affinity-purified polyclonal antibodies obtained from rabbits hyperimmunised with E. granulosus excretory/secretory antigens and biotinylated monoclonal antibody EmA9 produced against adult E. multilocularis somatic extract. The test was sensitive to 7 ng and 2.3 ng of E. granulosus protein and carbohydrate/ml of faecal supernatant, respectively. Thirteen helminth-free dogs were infected with different amounts of E. granulosus protoscoleces and the presence of coproantigen was monitored during the prepatent period until day 35 post-infection, when they were necropsied. Faecal antigen levels started to rise above the normal range between days 10 and 20 post-infection, and typically peaked at the end of the experiment. All the dogs, bearing from 3 to 67 700 worms, showed positive values in the ELISA during the prepatent period. One dog experimentally infected with Taenia hydatigena metacestode and harbouring three worms, tested positive only after the prepatent period at day 52. The test was applied to 98 stray dogs. The ELISA detected all of four dogs naturally infected with E. granulosus, two dogs with patent infections of T. hydatigena and two dogs with no cestode infections, showing a sensitivity of 100% and a specificity of 96%. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.
  • JA Abella, Y Oku, N Nonaka, M Ito, M Kamiya JOURNAL OF VETERINARY MEDICAL SCIENCE 59 (11) 1039 -1043 1997年11月 [査読無し][通常論文]
     
    Decrease of spleen weight from peak to control levels together with a corresponding decline of serum IgG level at the onset of gastric hyperplasia in Taenia taeniaeformis-infected euthymic rats, may indicate that a form of down regulation of host immune response during the course of larval T. taeniaeformis infection could facilitate the occurrence of gastropathy in rats. Gastric hyperplasia developed in T. taeniaeformis-infected athymic nude rats, indicating that occurrence of gastropathy associated with larval T. taeniaeformis infection in the rat is T cell independent. Apparently, gastric hyperplasia appeared early in nude rats which suggests that absence of T cell-mediated response could have facilitated its early occurrence.
  • JA Abella, Y Oku, N Nonaka, M Ito, M Kamiya JOURNAL OF VETERINARY MEDICAL SCIENCE 59 (11) 1039 -1043 1997年11月 [査読無し][通常論文]
     
    Decrease of spleen weight from peak to control levels together with a corresponding decline of serum IgG level at the onset of gastric hyperplasia in Taenia taeniaeformis-infected euthymic rats, may indicate that a form of down regulation of host immune response during the course of larval T. taeniaeformis infection could facilitate the occurrence of gastropathy in rats. Gastric hyperplasia developed in T. taeniaeformis-infected athymic nude rats, indicating that occurrence of gastropathy associated with larval T. taeniaeformis infection in the rat is T cell independent. Apparently, gastric hyperplasia appeared early in nude rats which suggests that absence of T cell-mediated response could have facilitated its early occurrence.
  • Jose Alexander Abella, Yuzaburo Oku, Kenjiro Konno, Zully Altamirano, Nariaki Nonaka, Masao Kamiya Parasitology International 46 (2) 97 -104 1997年 [査読無し][通常論文]
     
    Infection of hepatic larvae of Taenia taeniaeformis is associated with gastropathy in rats. The sequence of occurrence of the prominent changes, i.e. gastric hyperplasia, intragastric alkalinity and hypergastrinemia, could provide an insight on the pathogenetic mechanism(s) involved in the induction of gastric changes. Density of gastrin producing antral G cells was also examined to determine the cause of hypergastrinemia. Five-week-old, male Wistar rats were orally infected with approximately 4000 Taenia taeniaeformis eggs and examined for changes of stomach morphology, intragastric pH, and serum gastrin every 2 weeks post-infection. Numbers of antral G cells were determined by quantitative immunohistochemistry. Gastric hyperplasia, hypergastrinemia, and rise of intragastric pH occurred concomitantly 56 days post-infection. Mean number of G cells per 100 μm2 antral mucosa in infected rats was comparable to uninfected controls, although there was an apparent increase in width of antral mucosal sections in hyperplastic stomachs. The concomitant onset of gastric hyperplasia, intragastric alkalinity and hypergastrinemia, most likely indicate that chemical mediators are involved which primarily mediate rapid hyperplasia of mucus-secreting cells. Hypergastrinemia associated with this condition could be due to antral G-cell hyperfunction. However, an increase in total antral G-cell population should be considered because of gross enlargement of hyperplastic stomachs. © 1997 Elsevier Science Ireland Ltd.
  • ABE N, NONAKA N, IWAKI T, GANZORIG S, ALEXANDER J A, OKU Y, KAMIYA M Parasitology International 46 (1) 41 -44 1997年 [査読無し][通常論文]
  • 獣医寄生虫検査マニュアル 1.基礎編 2.寄生虫の検査・診断技術 2.6免疫学的検査法(6)ウエスタンブロット
    文永堂出版,東京 179 -182 1997年 [査読無し][通常論文]
  • Jose Alexander Abella, Yuzaburo Oku, Kenjiro Konno, Zully Altamirano, Nariaki Nonaka, Masao Kamiya Parasitology International 46 (2) 97 -104 1997年 [査読無し][通常論文]
     
    Infection of hepatic larvae of Taenia taeniaeformis is associated with gastropathy in rats. The sequence of occurrence of the prominent changes, i.e. gastric hyperplasia, intragastric alkalinity and hypergastrinemia, could provide an insight on the pathogenetic mechanism(s) involved in the induction of gastric changes. Density of gastrin producing antral G cells was also examined to determine the cause of hypergastrinemia. Five-week-old, male Wistar rats were orally infected with approximately 4000 Taenia taeniaeformis eggs and examined for changes of stomach morphology, intragastric pH, and serum gastrin every 2 weeks post-infection. Numbers of antral G cells were determined by quantitative immunohistochemistry. Gastric hyperplasia, hypergastrinemia, and rise of intragastric pH occurred concomitantly 56 days post-infection. Mean number of G cells per 100 μm2 antral mucosa in infected rats was comparable to uninfected controls, although there was an apparent increase in width of antral mucosal sections in hyperplastic stomachs. The concomitant onset of gastric hyperplasia, intragastric alkalinity and hypergastrinemia, most likely indicate that chemical mediators are involved which primarily mediate rapid hyperplasia of mucus-secreting cells. Hypergastrinemia associated with this condition could be due to antral G-cell hyperfunction. However, an increase in total antral G-cell population should be considered because of gross enlargement of hyperplastic stomachs. © 1997 Elsevier Science Ireland Ltd.
  • Parasitology International 46 (1) 41 -44 1997年 [査読無し][通常論文]
  • 獣医寄生虫検査マニュアル 1.基礎編 2.寄生虫の検査・診断技術 2.6免疫学的検査法(6)ウエスタンブロット
    文永堂出版,東京 179 -182 1997年 [査読無し][通常論文]
  • N Nonaka, M Iida, K Yagi, T Ito, HK Ooi, Y Oku, M Kamiya INTERNATIONAL JOURNAL FOR PARASITOLOGY 26 (11) 1271 -1278 1996年11月 [査読無し][通常論文]
     
    Coproantigen excretion during experimental infections of Echinococcus multilocularis in foxes and an alternative definitive host, golden hamsters, was evaluated by a sandwich ELISA using a monoclonal antibody. A sigmoidal increase of antigen excretion from the developing parasites was observed in in vitro incubation of the parasites collected on different days during the first 21 days post-infection (DPI). In hamsters, the ELISA O.D. value of faeces became positive at 4 DPI. Thereafter, the O.D. value increased in semi-sigmoidal fashion in the first 42 DPI, probably reflecting the development of the parasites. In foxes, the O.D. value became positive at 6 DPI. However, contrary to that in hamsters, after the initial steep rise, the O.D. value suddenly decreased to 1/2 the level during 15-17 DPI, indicating that a large number of worms might have been expelled. The parasite eggs were detected by the sugar centrifugal-flotation technique (Ito, Yagi & Ishige, 1989) from 29 to 84 DPI but not thereafter to 125 DPI, although mature parasites were detected at 125 DPI. In contrast, positive O.D. values were obtained almost constantly until 125 DPI, indicating that the coproantigen detection assay was more sensitive than the egg detection assay. The detection limit of the coproantigen detection assay was roughly estimated to be around 100 worms. These observations, along with the fact that the assay was designed to detect a heat-resistant coproantigen in heat-sterilized fecal samples, indicate that the coproantigen detection assay is a safe and useful method, not only for diagnosis in the definitive host of E. multilocularis, but also for monitoring parasite development and change in parasite burden during an experimental infection. Copyright (C) 1996 Australian Society for Parasitology. Published by Elsevier Science Ltd.
  • N Nonaka, M Iida, K Yagi, T Ito, HK Ooi, Y Oku, M Kamiya INTERNATIONAL JOURNAL FOR PARASITOLOGY 26 (11) 1271 -1278 1996年11月 [査読無し][通常論文]
     
    Coproantigen excretion during experimental infections of Echinococcus multilocularis in foxes and an alternative definitive host, golden hamsters, was evaluated by a sandwich ELISA using a monoclonal antibody. A sigmoidal increase of antigen excretion from the developing parasites was observed in in vitro incubation of the parasites collected on different days during the first 21 days post-infection (DPI). In hamsters, the ELISA O.D. value of faeces became positive at 4 DPI. Thereafter, the O.D. value increased in semi-sigmoidal fashion in the first 42 DPI, probably reflecting the development of the parasites. In foxes, the O.D. value became positive at 6 DPI. However, contrary to that in hamsters, after the initial steep rise, the O.D. value suddenly decreased to 1/2 the level during 15-17 DPI, indicating that a large number of worms might have been expelled. The parasite eggs were detected by the sugar centrifugal-flotation technique (Ito, Yagi & Ishige, 1989) from 29 to 84 DPI but not thereafter to 125 DPI, although mature parasites were detected at 125 DPI. In contrast, positive O.D. values were obtained almost constantly until 125 DPI, indicating that the coproantigen detection assay was more sensitive than the egg detection assay. The detection limit of the coproantigen detection assay was roughly estimated to be around 100 worms. These observations, along with the fact that the assay was designed to detect a heat-resistant coproantigen in heat-sterilized fecal samples, indicate that the coproantigen detection assay is a safe and useful method, not only for diagnosis in the definitive host of E. multilocularis, but also for monitoring parasite development and change in parasite burden during an experimental infection. Copyright (C) 1996 Australian Society for Parasitology. Published by Elsevier Science Ltd.
  • N Nonaka, BJ Thacker, TWS vanVeen, RW Bull VETERINARY PARASITOLOGY 61 (3-4) 181 -199 1996年02月 [査読無し][通常論文]
     
    In vitro maintenance of Eperythrozoon suis was attempted using a Petri dish erythrocyte culture system. In preliminary experiments, the optimal conditions for maintaining E. suis attachment to erythrocytes during incubation were anticoagulation with heparin or citrate solution, incubation with 5 or 10% CO2 at 37 degrees C, and incubation with reduced or non-reduced Eagle's minimum essential medium. Using heparin, a CO2 incubator and reduced Eagle's medium (rEM), E. suis metabolic activity was evaluated by measuring glucose consumption, and lactate and pyruvate production. Glucose consumption and lactate production were measurable while pyruvate production was not detected. Erythrocyte integrity was improved by the addition of inosine although no effect was observed on maintenance of E. suis attachment to erythrocytes or the rate of glucose consumption. To determine whether the glucose consumption observed in culture was due to E. suis glycolytic activity or enhanced erythrocyte glycolytic activity, the effect of E. suis killing by EDTA addition to medium was evaluated using rEM containing inosine (rEMI). Glucose consumption decreased proportionally with the decline in the percentage of parasitized erythrocytes induced by EDTA, indicating that glucose consumption was due to E. suis. In a subsequent experiment, the effect of different types of serum (pig or fetal calf serum) and different gaseous environments (5% CO2 incubator or candle jar) were evaluated using rEMI. Glucose consumption by E. suis was significantly increased by the addition of fetal calf serum; however, no difference in the maintenance of E. suis attachment to erythrocytes and in E. suis glycolytic activity was observed between a 5% CO2 incubator and a candle jar. Finally, the effect of medium refreshment (rEMI containing fetal calf serum) was evaluated, Maintenance of E. suis parasitism on erythrocytes and E. suis glycolytic activity were significantly improved by frequent medium refreshment. The maintenance system developed enabled successful metabolic radiolabeling of E. suis for protein/antigen analysis.
  • N Nonaka, BJ Thacker, TWS vanVeen, RW Bull VETERINARY PARASITOLOGY 61 (3-4) 181 -199 1996年02月 [査読無し][通常論文]
     
    In vitro maintenance of Eperythrozoon suis was attempted using a Petri dish erythrocyte culture system. In preliminary experiments, the optimal conditions for maintaining E. suis attachment to erythrocytes during incubation were anticoagulation with heparin or citrate solution, incubation with 5 or 10% CO2 at 37 degrees C, and incubation with reduced or non-reduced Eagle's minimum essential medium. Using heparin, a CO2 incubator and reduced Eagle's medium (rEM), E. suis metabolic activity was evaluated by measuring glucose consumption, and lactate and pyruvate production. Glucose consumption and lactate production were measurable while pyruvate production was not detected. Erythrocyte integrity was improved by the addition of inosine although no effect was observed on maintenance of E. suis attachment to erythrocytes or the rate of glucose consumption. To determine whether the glucose consumption observed in culture was due to E. suis glycolytic activity or enhanced erythrocyte glycolytic activity, the effect of E. suis killing by EDTA addition to medium was evaluated using rEM containing inosine (rEMI). Glucose consumption decreased proportionally with the decline in the percentage of parasitized erythrocytes induced by EDTA, indicating that glucose consumption was due to E. suis. In a subsequent experiment, the effect of different types of serum (pig or fetal calf serum) and different gaseous environments (5% CO2 incubator or candle jar) were evaluated using rEMI. Glucose consumption by E. suis was significantly increased by the addition of fetal calf serum; however, no difference in the maintenance of E. suis attachment to erythrocytes and in E. suis glycolytic activity was observed between a 5% CO2 incubator and a candle jar. Finally, the effect of medium refreshment (rEMI containing fetal calf serum) was evaluated, Maintenance of E. suis parasitism on erythrocytes and E. suis glycolytic activity were significantly improved by frequent medium refreshment. The maintenance system developed enabled successful metabolic radiolabeling of E. suis for protein/antigen analysis.
  • TSUKADA H, NONAKA N Mammal Study 21 (2) 137 -151 1996年 [査読無し][通常論文]
  • Parental strobilar development of Echinococcus multilocularis in scid mice (jointly worked)
    Japanese Journal of Veterinary Research 44 (1) 1 -12 1996年 [査読無し][通常論文]
  • Acquired resistance against adult Echinococcus multilocularis infection observed in golden hamsters (jointly worked)
    Japanese Jouranal of Parasitology 45 (1) 1 -5 1996年 [査読無し][通常論文]
  • Parental strobilar development of Echinococcus multilocularis in scid mice (jointly worked)
    Japanese Journal of Veterinary Research 44 (1) 1 -12 1996年 [査読無し][通常論文]
  • Acquired resistance against adult Echinococcus multilocularis infection observed in golden hamsters (jointly worked)
    Japanese Jouranal of Parasitology 45 (1) 1 -5 1996年 [査読無し][通常論文]
  • Antibody Production in Syphacia obvelata infected mice (jointly worked)
    Journal of Parasitology 81 (4) 559 -562 1995年 [査読無し][通常論文]
  • Antibody Production in Syphacia obvelata infected mice (jointly worked)
    Journal of Parasitology 81 (4) 559 -562 1995年 [査読無し][通常論文]
  • Developmental and Morphological characteristics of Taenia taeniaeformis (Batsch, 1786) in Clethrionomys rufocanus bedfordiae and Rattus norvegicus from different geographical locations (jointly worked)
    Journal of Parasitology 80 (3) 461 -467 1994年 [査読無し][通常論文]
  • 野中 成晃, 巌城 隆, 岡本 宗裕, ウイ ホンキエン, 奥 祐三部, 大林 正士, 神谷 正男 Journal of Veterinary Medical Sciences 56 (3) 565 -567 1994年 [査読無し][通常論文]
  • Developmental and Morphological characteristics of Taenia taeniaeformis (Batsch, 1786) in Clethrionomys rufocanus bedfordiae and Rattus norvegicus from different geographical locations (jointly worked)
    Journal of Parasitology 80 (3) 461 -467 1994年 [査読無し][通常論文]
  • Nariaki NONAKA, Takashi IWAKI, Munehiro OKAMOTO, Hong-Kean OOI, Yuzaburo OKU, Masashi OHBAYASHI, Masao KAMIYA Journal of Veterinary Medical Sciences 56 (3) 565 -567 1994年 [査読無し][通常論文]
  • Survey on larval Echinococcus multilocularis and other hepatic helminths in rodents and insectivores in Hokkaido, Japan, from 1985 to 1992 (jointly worked)
    Japanese Jouranal of Parasitology 42 (6) 502 -506 1993年 [査読無し][通常論文]
  • Survey on larval Echinococcus multilocularis and other hepatic helminths in rodents and insectivores in Hokkaido, Japan, from 1985 to 1992 (jointly worked)
    Japanese Jouranal of Parasitology 42 (6) 502 -506 1993年 [査読無し][通常論文]
  • N NONAKA, AR DONOGHUE, AM MANZONI, TWS VANVEEN AVIAN DISEASES 35 (3) 554 -558 1991年07月 [査読無し][通常論文]
     
    The prevalence of parasitic infections in backyard flocks was surveyed using litter samples from 74 pens located on 12 farms in central lower Michigan. Eight species of birds were represented. Two methods of litter examination (a sucrose flotation technique and a multiple washing/ZnCl2 flotation) were compared; the sucrose flotation technique was found to be more useful and was used in the survey. The following parasites eggs/oocysts were observed; ascarid-type eggs (in 34 pens from nine farms), Capillaria eggs (in 30 pens from nine farms), Strongyloides eggs (in nine pens from five farms), Syngamus eggs (in five pens from four farms), and coccidial oocysts (in 40 pens from 10 farms). Contamination of litter with ascarid-type eggs, Capillaria eggs, and coccidial oocysts was commonly found, irrespective of bird species. The contamination level in pens with more than one bird species was lower than in pens with a single species. The relatively high contamination rate may be an indication of the risk of parasitic disease in birds that are not raised under controlled conditions in confinement.
  • N NONAKA, AR DONOGHUE, AM MANZONI, TWS VANVEEN AVIAN DISEASES 35 (3) 554 -558 1991年07月 [査読無し][通常論文]
     
    The prevalence of parasitic infections in backyard flocks was surveyed using litter samples from 74 pens located on 12 farms in central lower Michigan. Eight species of birds were represented. Two methods of litter examination (a sucrose flotation technique and a multiple washing/ZnCl2 flotation) were compared; the sucrose flotation technique was found to be more useful and was used in the survey. The following parasites eggs/oocysts were observed; ascarid-type eggs (in 34 pens from nine farms), Capillaria eggs (in 30 pens from nine farms), Strongyloides eggs (in nine pens from five farms), Syngamus eggs (in five pens from four farms), and coccidial oocysts (in 40 pens from 10 farms). Contamination of litter with ascarid-type eggs, Capillaria eggs, and coccidial oocysts was commonly found, irrespective of bird species. The contamination level in pens with more than one bird species was lower than in pens with a single species. The relatively high contamination rate may be an indication of the risk of parasitic disease in birds that are not raised under controlled conditions in confinement.

共同研究・競争的資金等の研究課題

  • エキノコックス終宿主における免疫反応の解明
    地域先端技術共同研究開発
    研究期間 : 1993年 -2010年
  • エキノコックス症の疫学調査とその対策
    地域先端技術共同研究開発
    研究期間 : 1993年 -2010年
  • Analysis of Intestinal Immunity in the Definitive Hosts of Echinococcus Multilocularis
    0035 (Japanese Only)
    研究期間 : 1993年 -2010年
  • Epidemiology and Control of Echinococcosis
    0035 (Japanese Only)
    研究期間 : 1993年 -2010年
  • エキノコックス終宿主診断
    地域先端技術共同研究開発
    研究期間 : 1993年 -2005年
  • Diagnosis of the definitive host of Echinococcus by coproantigen detection.
    0035 (Japanese Only)
    研究期間 : 1993年 -2005年

大学運営

委員歴

  • 2004年   日本寄生虫学会北日本支部会   評議員   日本寄生虫学会北日本支部会
  • 2004年   日本寄生虫学会   評議員   日本寄生虫学会


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