研究者データベース

野中 成晃(ノナカ ナリアキ)
獣医学研究院 獣医学部門 病原制御学分野
教授

基本情報

所属

  • 獣医学研究院 獣医学部門 病原制御学分野

職名

  • 教授

学位

  • 博士(Ph.D.)(米国ミシガン州立大学)
  • 獣医学修士(北海道大学)

ホームページURL

J-Global ID

研究キーワード

  • 疫学   臨床寄生虫病学   寄生虫学   Epidemiology   Clinical Parasitology   Parasitology   

研究分野

  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 動物生産科学
  • ライフサイエンス / 動物生産科学

職歴

  • 2019年04月 - 現在 北海道大学 大学院獣医学研究院 教授
  • 2016年04月 - 2019年03月 宮崎大学 農学部 教授
  • 2008年09月 - 2016年03月 宮崎大学 農学部 獣医学科 准教授
  • 2004年09月 - 2008年08月 北海道大学 大学院獣医学研究科 講師
  • 1996年 - 2004年 北海道大学 大学院獣医学研究科 助手
  • 1993年 - 1996年 日本学術振興学 特別研究員

学歴

  •         - 1993年   ミシガン州立大学   大学院獣医学研究科   大動物臨床学
  •         - 1985年   北海道大学   獣医学部   獣医学
  •         - 1985年   北海道大学

所属学協会

  • 日本寄生虫学会北日本支部会   世界獣医寄生虫学会(World Association for the Advanced Veterinary Parasitologist)   日本寄生虫学会   日本獣医寄生虫学会   米国獣医寄生虫学会(American Association of Veterinary Parasitologist)   日本獣医学会   

研究活動情報

論文

  • Mohamed Abdallah Mohamed Moustafa, Hla Myet Chel, May June Thu, Saw Bawm, Lat Lat Htun, Mar Mar Win, Zaw Min Oo, Natsuo Ohsawa, Mirkka Lahdenperä, Wessam Mohamed Ahmed Mohamed, Kimihito Ito, Nariaki Nonaka, Ryo Nakao, Ken Katakura
    Scientific Reports 11 1 741 - 741 2021年12月 [査読有り][通常論文]
     
    AbstractHuman activities interfere with wild animals and lead to the loss of many animal populations. Therefore, efforts have been made to understand how wildlife can rebound from anthropogenic disturbances. An essential mechanism to adapt to environmental and social changes is the fluctuations in the host gut microbiome. Here we give a comprehensive description of anthropogenically induced microbiome alterations in Asian elephants (n = 30). We detected gut microbial changes due to overseas translocation, captivity and deworming. We found that microbes belonging to Planococcaceae had the highest contribution in the microbiome alterations after translocation, while Clostridiaceae, Spirochaetaceae and Bacteroidia were the most affected after captivity. However, deworming significantly changed the abundance of Flavobacteriaceae, Sphingobacteriaceae, Xanthomonadaceae, Weeksellaceae and Burkholderiaceae. These findings may provide fundamental ideas to help guide the preservation tactics and probiotic replacement therapies of a dysbiosed gut microbiome in Asian elephants. More generally, these results show the severity of anthropogenic activities at the level of gut microbiome, altering the adaptation processes to new environments and the subsequent capability to maintain normal physiological processes in animals.
  • Khanh-Linh Bui, Thu-Huong Nguyen, Hieu Duc Duong, Viet-Linh Nguyen, Thi-Nhien Nguyen, Lan-Anh Le, Ha-My Cong, Khanh-Trang Tran, Duc-Vinh Le, Eiji Nagayasu, Nariaki Nonaka, Ayako Yoshida
    Parasitology International 84 102405 - 102405 2021年10月 [査読有り][通常論文]
  • Shohei Ogata, Juan Antonio Cristian Pereira, Loza Vega Ariel Jhonny, Herbas Perez Gladys Carolina, Keita Matsuno, Yasuko Orba, Hirofumi Sawa, Fumihiko Kawamori, Nariaki Nonaka, Ryo Nakao
    Veterinary Sciences 8 9 188 - 188 2021年09月07日 [査読有り][通常論文]
     
    Latin American countries produce more than a quarter of the world’s beef and are a major global supplier of livestock protein. Tick-borne diseases (TBDs) are a major constraint to the livestock industry worldwide, including in Latin America. The aim of this study was to detect and characterise tick-borne pathogens in cattle from Santa Cruz, Bolivia, where no detailed epidemiological data are available. Blood samples were collected from 104 cattle. Apicomplexan parasites were detected by nested PCR amplification of the 18S ribosomal RNA gene (rDNA), and Anaplasmataceae was screened by the PCR amplification of 16S rDNA, followed by characterisation based on the heat shock protein and citrate synthase gene sequences. Babesia infection was observed in nine cattle (one Babesia bovis and eight Babesia bigemina), while Anaplasmataceae infection was detected in thirty-two cattle. A sequencing analysis confirmed the presence of Anaplasma marginale and Anaplasma platys-like. These results provide the first molecular evidence for the four above-mentioned tick-borne pathogens in cattle in Bolivia. This information improves our understanding of the epidemiology of TBDs and will help in formulating appropriate and improved pathogen control strategies.
  • Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, Samuel Kelava, Dayana Barker, Keita Matsuno, Nariaki Nonaka, Renfu Shao, Ben J. Mans, Stephen C. Barker, Ryo Nakao
    Ticks and Tick-borne Diseases 101832 - 101832 2021年09月
  • Saw Bawm, Tin Tin Myaing, May June Thu, Shirin Akter, Lat Lat Htun, Mar Mar Win, Nariaki Nonaka, Ryo Nakao, Ken Katakura
    Ticks and Tick-borne Diseases 12 4 101729 - 101729 2021年07月 [査読有り][通常論文]
     
    Canine vector-borne pathogens can act as zoonotic agents in humans; however, it poorly understood whether dogs play a role as reservoirs of vector-borne parasites in livestock animals. Here, we report the unexpected detection of 18S rRNA gene (rDNA) sequences of five ruminant Theileria species from the peripheral blood of dogs in Myanmar, in addition to those of two canine Babesia species. Using novel BTH primers capable of amplifying the 18S rDNA of Babesia, Theileria, and Hepatozoon spp., approximately 1,500 bp nested PCR products were detected in 19% (17/91) of local or imported dog breeds in different regions of Myanmar. Among the sequences of the 17 PCR products, ten were determined as Theileria 18S rDNA, including three as Theileria orientalis, three as Theileria buffeli, two as Theileria cf. velifera, one as Theileria luwenshuni, and one as Theileria sp. Most of these sequences showed higher identities with Theileria sequences determined in previous studies of cattle, water buffaloes, and goats in Myanmar. Six PCR products were identified as Babesia vogeli and one sample was determined as Babesia gibsoni. Furthermore, we obtained approximately 900 bp thrombospondin-related adhesive protein (TRAP) gene fragments from three dog blood DNA samples. Phylogenetic analysis of the TRAP gene showed that B. gibsoni parasites in Myanmar were considerably related to isolates from China, Korea, Japan, and Taiwan, but clearly separated from those from Bangladesh and India. These results provide new insights into a possible role of dogs in maintaining and spreading tick-borne pathogens among livestock and canine populations in Myanmar.
  • Toshiya Kobayashi, Elisha Chatanga, Yongjin Qiu, Martin Simuunza, Masahiro Kajihara, Bernard Mudenda Hang’ombe, Yoshiki Eto, Ngonda Saasa, Akina Mori-Kajihara, Edgar Simulundu, Ayato Takada, Hirofumi Sawa, Ken Katakura, Nariaki Nonaka, Ryo Nakao
    Pathogens 10 6 779 - 779 2021年06月21日 [査読有り][通常論文]
     
    Ticks are obligate ectoparasites as they require to feed on their host blood during some or all stages of their life cycle. In addition to the pathogens that ticks harbor and transmit to vertebrate hosts, they also harbor other seemingly nonpathogenic microorganisms including nutritional mutualistic symbionts. Tick nutritional mutualistic symbionts play important roles in the physiology of the host ticks as they are involved in tick reproduction and growth through the supply of B vitamins as well as in pathogen maintenance and propagation. Coxiella-like endosymbionts (CLEs) are the most widespread endosymbionts exclusively reported in ticks. Although CLEs have been investigated in ticks in other parts of the world, there is no report of their investigation in ticks in Zambia. To investigate the occurrence of CLEs, their maintenance, and association with host ticks in Zambia, 175 ticks belonging to six genera, namely Amblyomma, Argas, Haemaphysalis, Hyalomma, Ornithodoros, and Rhipicephalus, were screened for CLEs, followed by characterization of CLEs by multi-locus sequence typing of the five Coxiella housekeeping genes (dnaK, groEL, rpoB, 16S rRNA, and 23S rRNA). The results showed that 45.7% (n = 80) were positive for CLEs. The comparison of the tick 16S rDNA phylogenetic tree with that of the CLEs concatenated sequences showed that there was a strong correlation between the topology of the trees. The results suggest that most of the CLEs have evolved within tick species, supporting the vertical transmission phenomenon. However, the negative results for CLE in some ticks warrants further investigations of other endosymbionts that the ticks in Zambia may also harbor.
  • Saw Bawm, Rumi Sagara, Keita Kakisaka, May June Thu, Myint Myint Hmoon, Lat Lat Htun, Mar Mar Win, Nariaki Nonaka, Ryo Nakao, Hitoshi Suzuki, Ken Katakura
    Parasitology International 82 102299 - 102299 2021年06月 [査読有り][通常論文]
     
    Ribosomal RNA genes have been widely used for the identification and phylogenetic analysis of various organisms, including parasitic protozoa. Here, we report nine near full-length Theileria orientalis 18S rRNA gene sequences from cattle from different areas of Myanmar. Phylogenetic analysis of the 18S rRNA genes revealed a considerably close genetic relationship among T. orientalis isolates from Australia, China, Japan, Korea, Myanmar, and Pakistan. We also obtained four Theileria velifera-like (Theileria cf. velifera) 18S rRNA gene sequences from two cattle and two water buffaloes from the northernmost area of Myanmar. The phylogenetic analysis of T. cf. velifera isolates from Myanmar along with T. velifera and T. cf. velifera isolates from African countries suggested an evolutionary lineage of greater complexity in T. velifera-related parasites. DNA alignment analysis indicated the presence of 51 and 55 nucleotide variation positions within the 18S rRNA genes from 15 T. orientalis and 11 T. velifera-related isolates, respectively. Alignment entropy analysis of the 18S rRNA sequences indicated that both T. orientalis and T. velifera-related isolates had three hyper variable regions, corresponding to V2, V4, and V7 regions in eukaryotes. The degree of variation was prominent in the V2 in T. orientalis and V4 in T. velifera-related isolates. The secondary structure analysis of the 18S rRNA predicted using minimum free energy algorism revealed that the structure of V4 region differed most significantly between T. orientalis and T. velifera. These results provide novel insights into common structures, variations and functions of small subunit rRNA in Theileria species.
  • Yurie Taya, Gohta Kinoshita, Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, Shohei Ogata, Elisha Chatanga, Yuma Ohari, Kodai Kusakisako, Keita Matsuno, Nariaki Nonaka, Ryo Nakao
    Microorganisms 9 5 1051 - 1051 2021年05月13日 [査読有り][通常論文]
     
    Ticks serve as important vectors of a variety of pathogens. Recently, the viral and prokaryotic microbiomes in ticks have been explored using next-generation sequencing to understand the physiology of ticks and their interactions with pathogens. However, analyses of eukaryotic communities in ticks are limited, owing to the lack of suitable methods. In this study, we developed new methods to selectively amplify microeukaryote genes in tick-derived DNA by blocking the amplification of the 18S rRNA gene of ticks using artificial nucleic acids: peptide nucleic acids (PNAs) and locked nucleic acids (LNAs). In addition, another PCR using non-metazoan primers, referred to as UNonMet-PCR, was performed for comparison. We performed each PCR using tick-derived DNA and sequenced the amplicons using the Illumina MiSeq platform. Almost all sequences obtained by conventional PCR were derived from ticks, whereas the proportion of microeukaryotic reads and alpha diversity increased upon using the newly developed method. Additionally, the PNA- or LNA-based methods were suitable for paneukaryotic analyses, whereas the UNonMet-PCR method was particularly sensitive to fungi. The newly described methods enable analyses of the eukaryotic microbiome in ticks. We expect the application of these methods to improve our understanding of the tick microbiome.
  • Naoki Hayashi, Hirokazu Kouguchi, Yuhei Imasato, Takao Irie, Kinpei Yagi, Nariaki Nonaka, Ryo Nakao
    International Journal for Parasitology 2021年05月
  • Yuhei Imasato, Ryo Nakao, Takao Irie, Hirokazu Kouguchi, Kinpei Yagi, Nonaka Nariaki, Ken Katakura
    Parasitology International 81 102247 - 102247 2021年04月 [査読有り][通常論文]
  • Yuma Ohari, Kayoko Matsuo, Ayako Yoshida, Nariaki Nonaka, Hiroshi Sato, Tadashi Itagaki
    Parasitology Research 120 4 1341 - 1350 2021年04月 [査読有り][通常論文]
     
    Understanding the population structure of Fasciola flukes in domestic and wild animals is important for determining the extent of cross-infection between them. Although the parthenogenetic Fasciola flukes in Japan have been shown to comprise five genetic types based on the ribosomal internal transcribed spacer 1 (ITS1) and mitochondrial NADH dehydrogenase subunit 1 (nad1) regions, these genetic regions are not suitable for analyzing their population structure. In the present study, the genetic diversity and population structure of the parthenogenetic Fasciola flukes in Japan were studied using microsatellite DNA, ITS1, and nad1 regions. A total of 144 parthenogenetic Fasciola flukes, obtained from cattle and sika deer in 16 localities, were individually analyzed using PCR-RFLP for ITS1, PCR-direct sequence analysis for nad1, and post-labeling PCR and capillary electrophoresis for microsatellite DNA regions. The flukes showed higher genetic diversity in the microsatellite DNA regions than ITS1 and nad1. The population structures of parthenogenetic Fasciola flukes were unclear, however, it was suggested that the flukes are more diverse populations. We hypothesized that their distribution throughout Japan is closely related to livestock movement dependent on human activity. Moreover, it is considered that cross-infection of the flukes between cattle and sika deer possibly has occurred in the past.
  • Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens
    Yen Thi Hoang Nguyen, Nariaki Nonaka, Haruhiko Maruyama, Ayako Yoshida
    Journal of Veterinary Medical Science 83 4 671 - 674 2021年04月 [査読有り][通常論文]
  • Wessam Mohamed Ahmed Mohamed, Alsagher O. Ali, Hassan Y. A. H. Mahmoud, Mosaab A. Omar, Elisha Chatanga, Bashir Salim, Doaa Naguib, Jason L. Anders, Nariaki Nonaka, Mohamed Abdallah Mohamed Moustafa, Ryo Nakao
    Pathogens 10 3 351 - 351 2021年03月16日 [査読有り][通常論文]
     
    Dromedary camels (Camelus dromedarius) are widely distributed in Africa, the Middle East and northern India. In this study, we aimed to detect tick-borne pathogens through investigating prokaryotic and eukaryotic microorganisms in camel blood based on a metagenomic approach and then to characterize potentially pathogenic organisms using traditional molecular techniques. We showed that the bacteria circulating in the blood of camels is dominated by Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. At the genus level, Sediminibacterium, Hydrotalea, Bradyrhizobium and Anaplasma were the most abundant taxa. Eukaryotic profile was dominated by Fungi, Charophyta and Apicomplexa. At the genus level, Theileria was detected in 10 out of 18 samples, while Sarcocystis, Hoplorhynchus and Stylocephalus were detected in one sample each. Our metagenomic approach was successful in the detection of several pathogens or potential pathogens including Anaplasma sp., Theileria ovis, Th. separata, Th. annulate, Th. mutans-like and uncharacterized Theileria sp. For further characterization, we provided the partial sequences of citrate synthase (gltA) and heat-shock protein (groEL) genes of Candidatus Anaplasma camelii. We also detected Trypanosoma evansi type A using polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) region. This combined metagenomic and traditional approach will contribute to a better understanding of the epidemiology of pathogens including tick-borne bacteria and protozoa in animals.
  • Elisha Chatanga, Henson Kainga, Emmanuel Maganga, Kyoko Hayashida, Ken Katakura, Chihiro Sugimoto, Nariaki Nonaka, Ryo Nakao
    Ticks and Tick-borne Diseases 12 2 101629 - 101629 2021年03月 [査読有り][通常論文]
     
    Tick-borne diseases (TBDs) caused by pathogens belonging to the genera Anaplasma, Ehrlichia, Babesia and Theileria in small ruminants are widespread in the tropical and sub-tropical countries. The epidemiology of tick-borne pathogens (TBPs) in small ruminants is less understood compared to those infecting cattle in general. This study was carried out to investigate and characterize TBPs in sheep and goats using molecular tools. A total of 107 blood samples from sheep (n = 8) and goats (n = 99) were collected from animals that were apparently healthy from two farms in the central and the southern regions of Malawi. The V4 hypervariable region of the 18S ribosomal RNA gene (rDNA) and the V1 hypervariable region of the 16S rDNA polymerase chain reaction (PCR) assays were used for detection of tick-borne piroplasms and Anaplasmataceae, respectively. Almost the full-length 18S rDNA and the heat shock protein (groEL) gene sequences were used for genetic characterization of the piroplasms and Anaplasmataceae, respectively. The results showed that 76.6 % of the examined animals (n = 107) were positive for at least one TBP. The overall co-infection with at least two TBPs was observed in forty-eight animals (45 %). The detected TBPs were Anaplasma ovis (65 %), Ehrlichia ruminantium (4%), Ehrlichia canis (2%), Babesia strain closely related to Babesia gibsoni (1%), Theileria ovis (52 %), Theileria mutans (3%), Theileria separata (2%), Anaplasma sp. (1%) and Theileria sp. strain MSD-like (17 %). To the authors knowledge this is the first molecular study of TBPs in sheep and goats in Malawi. These results have therefore provided a significant milestone in the knowledge of occurrence of TBPs in sheep and goats in Malawi, which is prerequisite to proper diagnosis and control.
  • Makoto Nagai, Tamaki Okabayashi, Masataka Akagami, Aya Matsuu, Yoshikazu Fujimoto, Md Abul Hashem, Hirohisa Mekata, Ryo Nakao, Keita Matsuno, Yukie Katayama, Mami Oba, Tsutomu Omatsu, Tetsuo Asai, Keisuke Nakagawa, Hiroshi Ito, Hiroo Madarame, Kazuhiro Kawai, Toshihiro Ito, Nariaki Nonaka, Kyoko Tsukiyama-Kohara, Yasuo Inoshima, Tetsuya Mizutani, Naoaki Misawa
    Infection, Genetics and Evolution 88 104664 - 104664 2021年03月 [査読有り][通常論文]
     
    Recently, hepe-astrovirus-like RNA viruses named bastroviruses (BastVs), have been found in human, pig, bat, and rat fecal samples. In this study, we determined nearly complete genome sequences of four BastVs in the feces of healthy pigs. Genetic characterization revealed that these porcine BastVs (PBastVs) and BastVs from other animals including humans, had the same genome organization, that is, they contained three predicted conserved domains of viral methyltransferase, RNA helicase, and RdRp in the nonstructural ORF1 and the astrovirus capsid domain in the structural ORF2. Phylogenetic analyses using RNA-dependent RNA polymerase and the capsid region revealed that PBastVs branched with bat and rat BastVs; however, the groups formed by each host were distantly related to human BastVs. Pairwise amino acid sequence comparison demonstrated that PBastVs shared 95.2-98.6% and 76.1-95.5% sequence identity among each other in the ORF1 and ORF2 regions, respectively; the sequence identities between PBastVs and BastVs from other animals were 21.4-42.5% and 9.1-20.6% in the ORF1 and ORF2 regions, respectively. This suggested that BastVs were derived from a common ancestor but evolved independently in each host population during a prolonged period. Putative recombination events were identified in the PBastV genome, suggesting that PBastVs gain sequence diversity and flexibility through recombination events. In an analysis of previously obtained metagenomic data, PBastV sequence reads were detected in 7.3% (23/315) of fecal samples from pigs indicating that PBastVs are distributed among pig populations in Japan.
  • Shohei Ogata, Wessam Mohamed Ahmed Mohamed, Kodai Kusakisako, May June Thu, Yongjin Qiu, Mohamed Abdallah Mohamed Moustafa, Keita Matsuno, Ken Katakura, Nariaki Nonaka, Ryo Nakao
    Microorganisms 9 2 333 - 333 2021年02月08日 [査読有り][通常論文]
     
    Members of the genus Spiroplasma are Gram-positive bacteria without cell walls. Some Spiroplasma species can cause disease in arthropods such as bees, whereas others provide their host with resistance to pathogens. Ticks also harbour Spiroplasma, but their role has not been elucidated yet. Here, the infection status and genetic diversity of Spiroplasma in ticks were investigated using samples collected from different geographic regions in Japan. A total of 712 ticks were tested for Spiroplasma infection by PCR targeting 16S rDNA, and Spiroplasma species were genetically characterized based on 16S rDNA, ITS, dnaA, and rpoB gene sequences. A total of 109 samples originating from eight tick species were positive for Spiroplasma infection, with infection rates ranging from 0% to 84% depending on the species. A linear mixed model indicated that tick species was the primary factor associated with Spiroplasma infection. Moreover, certain Spiroplasma alleles that are highly adapted to specific tick species may explain the high infection rates in Ixodes ovatus and Haemaphysalis kitaokai. A comparison of the alleles obtained suggests that horizontal transmission between tick species may not be a frequent event. These findings provide clues to understand the transmission cycle of Spiroplasma species in wild tick populations and their roles in host ticks.
  • Ryo Nakao, Kohei Shinjo, Tomoki Sakiyama, Shohei Ogata, Kodai Kusakisako, Gohta Kinoshita, Doaa Naguib, Elisha Chatanga, Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, Keita Matsuno, Takuya Ito, Nariaki Nonaka, Mariko Sashika, Toshio Tsubota, Michito Shimozuru
    Parasitology International 80 102209 - 102209 2021年02月 [査読有り][通常論文]
     
    The tick Amblyomma testudinarium Koch, 1844 (Acari: Ixodidae) is known as a vector of several pathogens such as Rickettsia tamurae and severe fever with thrombocytopenia syndrome (SFTS) virus. This tick species is present in many Asian countries, including Japan, where its distribution is limited to the warm areas of Kanto region and the southwestern region. The present study reports the recovery of a partially engorged A. testudinarium from a wild brown bear captured in Shari town, Hokkaido. In addition to morphological identification, the specimen was genetically characterized by the complete mitochondrial genome sequencing. The results showed that the length of the obtained mitogenome is 14,835 bp that encodes 13 protein-coding, two ribosomal RNA (rRNA) (12S and 16S), and 22 transfer RNA genes with two non-coding control regions. The phylogenetic analysis indicated that our sample clustered with A. testudinarium from Nara, Japan, but separated from A. testudinarium from China. Although the introduction of the tick through livestock transportation cannot be ruled out, the detection of A. testudinarium in Hokkaido prefecture, which is separated from the main island where A. testudinarium is present in the south, may suggest the introduction by migratory birds. This study provides important insights on the distribution and host range of A. testudinarium. This will be useful for the future taxonomic analysis of ticks based on the complete mitogenome sequencing. To our knowledge, this is the northernmost detection point of the tropical tick A. testudinarium.
  • Elisha Chatanga, Henson Kainga, Tinotenda Razemba, Richard Ssuna, Lieza Swennen, Kyoko Hayashida, Chihiro Sugimoto, Ken Katakura, Nariaki Nonaka, Ryo Nakao
    Parasitology Research 120 1 267 - 276 2021年01月 [査読有り][通常論文]
     
    Tick-borne pathogens (TBPs) in dogs have attracted much attention over the last decade since some are now known to be zoonotic and pose a threat to both animal and human health sectors. Despite the increase in the number of studies on canine TBPs worldwide, only a few studies have been conducted in resource-limited countries where research priority is given to food animals than companion animals. In the present study, the occurrence of TBPs of the genera Babesia, Hepatozoon, Anaplasma, and Ehrlichia was investigated in 209 owned and stray dogs in three major cities in Malawi through molecular techniques. Among the examined dogs, 93 (44.5%) were infected with at least one TBP. The detection rates were 23.1% for Babesia rossi, 2.9% for B. vogeli, 19.1% for Hepatozoon canis, 2.4% for Anaplasma platys, and 3.8% for Ehrlichia canis. This is the first molecular study that has provided evidence that dogs in Malawi are infected with TBPs. Sensitization is required for veterinary practitioners, dog handlers, and pet owners as the detected pathogens affect the animals' wellbeing. Further studies focusing on rural areas with limited or no access to veterinary care are required to ascertain the extent of the TBP infection in dogs.
  • Shwe Yee Win, Hla Myet Chel, Myint Myint Hmoon, Lat Lat Htun, Saw Bawm, Mar Mar Win, Shiro Murata, Nariaki Nonaka, Ryo Nakao, Ken Katakura
    Acta Tropica 212 105719 - 105719 2020年12月 [査読有り][通常論文]
     
    Village chicken production, a traditional, small-scale, and extensive backyard poultry industry, has been profitable for local farmers in Myanmar. However, there is scanty information available concerning the infection of these chickens with avian pathogens, including haemoprotozoan parasites. In the present study, we provide the first report of microscopic detection and molecular identification of Leucocytozoon and Plasmodium parasites from seven different areas of Myanmar. Leucocytozoon gametocytes were detected in 17.6% (81/461) of the blood smears from village chickens. The nested polymerase chain reaction (PCR) for targeting Leucocytozoon mitochondrial cytochrome b (cyt b) genes had a 17.6% positive rate. Although the positive rate of nested PCR targeting Plasmodium/Haemoproteus cyt b was 34.3%, the PCR protocol was observed to possibly amplify DNA of a certain species of Leucocytozoon. There were no obvious clinical signs in the infected birds. Statistical analysis of the microscopic detection and PCR detection rates using the age and sex of birds as internal factors revealed that the statistical significances differed according to the study area. The sequencing of 32 PCR products obtained from each study area revealed infection by Leucocytozoon caulleryi in three birds, Leucocytozoon sabrazesi in two birds, Leucocytozoon schoutedeni in two birds, Leucocytozoon sp. in eighteen birds, and Plasmodium juxtanucleare in seven birds; however, Haemoproteus infection was not detected. While L. sabrazesi was detected in chickens from the central region of Myanmar, the other haemosporidians were detected in those from different areas. In the haplotype analysis, we detected 17 haemosporidian cyt b haplotypes, including two for L. caulleryi, one for L. sabrazesi, two for L. schoutedeni, nine for Leucocytozoon sp., and three for P. juxtanucleare. Phylogenetic analysis of the cyt b haplotypes revealed a considerably close genetic relationship among chicken haemosporidians detected in Myanmar, Thailand, and Malaysia. These results indicate that well-recognized widespread species of chicken Leucocytozoon and Plasmodium are distributed nationwide in Myanmar, providing new insights into the ecosystem and control strategies of haemosporidian parasites in domesticated chickens in Myanmar.
  • 北海道斜里町においてエゾヒグマより採集されたタカサゴキララマダニの一例
    下鶴 倫人, 新庄 康平, 崎山 智樹, 小方 昌平, 草木迫 浩大, 木下 豪太, Mohamed Wessam Mohamed Ahmed, Moustafa Mohamed Abdallah Mohamed, 松野 啓太, 伊東 拓也, 野中 成晃, 佐鹿 万里子, 坪田 敏男, 中尾 亮
    日本獣医学会学術集会講演要旨集 163回 327 - 327 (公社)日本獣医学会 2020年10月
  • ナイロンメッシュの利用による膵蛭類の虫卵検査法の改善とそれを利用した駆虫試験
    坂口浩平, 甘甜甜, 船倉栄, 新田芳樹, 昌子暢賢, 山本裕子, 酒井博史, 堀井洋一郎, 野中成晃
    日本獣医寄生虫学会誌 19 1 30 - 39 2020年06月 [査読有り][通常論文]
  • Christopher Adenyo, Kenji Ohya, Yongjin Qiu, Yasuhiro Takashima, Hirohito Ogawa, Tateki Matsumoto, May June Thu, Kozue Sato, Hiroki Kawabata, Yukie Katayama, Tsutomu Omatsu, Tetsuya Mizutani, Hideto Fukushi, Ken Katakura, Narikaki Nonaka, Miho Inoue-Murayama, Boniface Kayang, Ryo Nakao
    Acta Tropica 205 105388 - 105388 2020年05月 [査読有り][通常論文]
  • Elisha Chatanga, Kyoko Hayashida, Walter Muleya, Kodai Kusakisako, Mohamed Abdallah Mohamed Moustafa, Bashir Salim, Ken Katakura, Chihiro Sugimoto, Nariaki Nonaka, Ryo Nakao
    Pathogens 9 5 334 - 334 2020年04月30日 [査読有り][通常論文]
     
    East Coast fever (ECF) is an acute fatal tick-borne disease of cattle caused by Theileria parva. It causes major losses in exotic and crossbreed cattle, but this could be prevented by a vaccine of T. parva if the vaccine is selected properly based on information from molecular epidemiology studies. The Muguga cocktail (MC) vaccine (Muguga, Kiambu 5 and Serengeti-transformed strains) has been used on exotic and crossbreed cattle. A total of 254 T. parva samples from vaccinated and unvaccinated cattle were used to understand the genetic diversity of T. parva in Malawi using partial sequences of the Tp1 and Tp2 genes encoding T. parva CD8+ antigens, known to be immunodominant and current candidate antigens for a subunit vaccine. Single nucleotide polymorphisms were observed at 14 positions (3.65%) in Tp1 and 156 positions (33.12%) in Tp2, plus short deletions in Tp1, resulting in 6 and 10 amino acid variants in the Tp1 and Tp2 genes, respectively. Most sequences were either identical or similar to T. parva Muguga and Kiambu 5 strains. This may suggest the possible expansion of vaccine components into unvaccinated cattle, or that a very similar genotype already existed in Malawi. This study provides information that support the use of MC to control ECF in Malawi.
  • Hirokazu Kouguchi, Hidefumi Furuoka, Takao Irie, Jun Matsumoto, Ryo Nakao, Nariaki Nonaka, Yasuyuki Morishima, Kazuhiro Okubo, Kinpei Yagi
    Data in Brief 29 105353 - 105353 2020年04月 [査読有り][通常論文]
  • Hla Myet Chel, Takashi Iwaki, Myint Myint Hmoon, Yu Nandi Thaw, Nyein Chan Soe, Shwe Yee Win, Saw Bawm, Lat Lat Htun, Mar Mar Win, Zaw Min Oo, Md Abdul Masum, Osamu Ichii, Ryo Nakao, Nariaki Nonaka, Ken Katakura
    International Journal for Parasitology: Parasites and Wildlife 11 294 - 301 2020年04月 [査読有り]
  • Hla Myet Chel, Ryo Nakao, Natsuo Ohsawa, Zaw Min Oo, Nariaki Nonaka, Ken Katakura
    Parasitology International 75 102035 - 102035 2020年04月 [査読有り][通常論文]
     
    The stomach bot fly species in Asian elephants has long been known as Cobboldia elephantis. However, there is no genetic information available for this species to date. Here, we report that a third-instar fly larva was excreted from a captive Asian elephant four months after export from an elephant camp in Myanmar to a zoological garden in Japan. Morphological characteristics of the larva were coincident with published descriptions of C. elephantis. The mitochondrial cytochrome c oxidase subunit I (COI) gene was amplified from the larva by PCR using primers modified from those designed for DNA barcoding of insects and amphibians. The COI gene of C. elephantis showed 76.6 % and 83.6 % identity at the nucleotide and amino acid levels, respectively, to that of C. loxodontis, the stomach bot fly species in African elephants. Phylogenetic analysis of the COI genes of several stomach bot fly species revealed that the two Cobboldia species formed a clade separate from the stomach bot fly species found in rhinoceros and equids.
  • Yen Thi Hoang Nguyen, Yayoi Hayata, Shino Sonoda, Nariaki Nonaka, Haruhiko Maruyama, Ayako Yoshida
    Parasitology International 75 102022 - 102022 2020年04月 [査読有り][通常論文]
  • Phoo Pwint Ko, Kohei Sakaguchi, Ayako Yoshida, Haruhiko Maruyama, Nariaki Nonaka, Eiji Nagayasu
    Parasitology International 72 101937 - 101937 2019年10月 [査読有り][通常論文]
  • May June Thu, Yongjin Qiu, Junya Yamagishi, Kodai Kusakisako, Shohei Ogata, Mohamed Abdallah Mohamed Moustafa, Norikazu Isoda, Chihiro Sugimoto, Ken Katakura, Nariaki Nonaka, Ryo Nakao
    Microbiology Resource Announcements 8 37 2019年09月12日 [査読有り][通常論文]
     
    This is the first report of the complete genome sequence of Rickettsia asiatica strain Maytaro1284, isolated from an Ixodes ovatus tick in Japan. The genome contains a 1,344,324-bp circular chromosome and one plasmid of 74,761 bp. There was no outer membrane protein A ( ompA ) gene encoded in the genome.
  • Theileria parva CD8+抗原をコードする2遺伝子の配列から、マラウイのワクチン非接種牛におけるMugugaワクチン株の存在がわかる(Sequences of two genes encoding Theileria parva CD8+ antigens reveal the presence of Muguga vaccine strain in non-vaccinated cattle in Malawi)
    Chatanga Elisha, 中尾 亮, 林田 京子, Bashir Salim, 杉本 千尋, 片倉 賢, 野中 成晃
    日本獣医学会学術集会講演要旨集 162回 360 - 360 2019年08月
  • Riki SISWANDI, Ayako YOSHIDA, Hiroyuki SATOH, Nariaki NONAKA
    Journal of Veterinary Medical Science 81 7 1021 - 1028 2019年 [査読有り][通常論文]
  • Hirohisa Mekata, Tomoya Minamino, Yoko Mikurino, Mari Yamamoto, Ayako Yoshida, Nariaki Nonaka, Yoichiro Horii
    Veterinary Parasitology 263 1 - 4 2018年11月 [査読有り][通常論文]
  • Zhenzhen Wang, Miho Shibata, Yen Thi Hoang Nguyen, Yayoi Hayata, Nariaki Nonaka, Haruhiko Maruyama, Ayako Yoshida
    Parasitology International 67 5 622 - 626 2018年10月 [査読有り][通常論文]
  • Maki Sekiguchi, Nariaki Nonaka, Mamoru Adachi, Tamami Sekiya, Yu Yamamoto
    Avian Diseases 62 3 326 - 329 2018年09月 [査読有り][通常論文]
     
    In May 2017, a hen in a backyard chicken flock in Japan exhibited mild clinical signs, and the bird was examined for diagnosis. Unexpectedly, many microfilariae were observed in the lung by histologic examination, although no adult worms were detected within the body. In a blood test performed in July, microfilaremia was confirmed in a few clinically normal chickens of the same flock. Molecular analysis of the nematode partial 18S ribosomal RNA gene revealed that the gene detected in the lung of the necropsied hen was positioned in the group of the family Onchocercidae in the phylogenetic tree. These data show that avian filarioids that can infect chickens inhabit Japan.
  • 日本産肝蛭の集団遺伝学的解析 マイクロサテライトマーカーを用いた分布拡大の解明
    尾針 由真, 林 慶, Mohanta Uday Kumar, 入江 隆夫, 松尾 加代子, 吉田 彩子, 野中 成晃, 佐藤 宏, 板垣 匡
    日本獣医学会学術集会講演要旨集 161回 324 - 324 (公社)日本獣医学会 2018年08月
  • Rahpaya Sayed Samim, Tsuchiaka Shinobu, Kishimoto Mai, Oba Mami, Katayama Yukie, Nunomura Yuka, Kokawa Saki, Kimura Takashi, Kobayashi Atsushi, Kirino Yumi, Okabayashi Tamaki, Nonaka Nariaki, Mekata Hirohisa, Aoki Hiroshi, Shiokawa Mai, Umetsu Moeko, Morita Tatsushi, Hasebe Ayako, Otsu Keiko, Asai Tetsuo, Yamaguchi Tomohiro, Makino Shinji, Murata Yoshiteru, Abi Ahmad Jan, Omatsu Tsutomu, Mizutani Tetsuya
    JOURNAL OF VETERINARY SCIENCE 19 3 350 - 350 2018年 [査読有り][通常論文]
     
    Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, Salmonella enterica ser. Typhimurium, and Neospora caninum; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time.
  • Kai FENG, Wei LI, Zhihong GUO, Hong DUO, Yong FU, Xiuying SHEN, Cheng TIE, Rijie E, Changqin XIAO, Yanhong LUO, Guo QI, Ma NI, Qingmei MA, Wataru YAMAZAKI, Ayako YOSHIDA, Yoichiro HORII, Kinpei YAGI, Nariaki NONAKA
    Journal of Veterinary Medical Science 79 12 1986 - 1993 2017年12月 [査読有り][通常論文]
  • IgG antibody development in chickens infected with Toxocara canis, Toxocara cati, Ascaris suum, and Ascaridia galli by enzyme-linked immunosorbent assay
    Yen Thi Hoang Nguyen, Haruhiko Maruyama, Ayako Yoshida, Nariaki Nonaka
    Journal of Malaria and Parasitic Disease Control 102 6 9 - 17 2017年12月 [査読有り][通常論文]
  • 日本産肝蛭の集団遺伝学的解析 マイクロサテライトマーカーを用いたクローンの多様性
    尾針 由真, 林 慶, Mohanta Uday Kumar, 入江 隆夫, 松尾 加代子, 吉田 彩子, 野中 成晃, 佐藤 宏, 板垣 匡
    日本獣医学会学術集会講演要旨集 160回 342 - 342 (公社)日本獣医学会 2017年08月
  • Takao Irie, Takuya Ito, Hirokazu Kouguchi, Kimiaki Yamano, Kohji Uraguchi, Kinpei Yagi, Nariaki Nonaka
    Parasitology Research 116 8 2139 - 2144 2017年08月 [査読有り][通常論文]
     
    The use of copro-DNA detection methods for the diagnosis of canine Echinococcus multilocularis infection was evaluated with a focus on DNA extraction techniques: two commercial kits and a modified alkaline-sodium dodecyl sulfate (SDS) technique. Dog feces (0.2 g) mixed with a protoscolex or with 1 or 10 eggs of E. multilocularis were subjected to DNA detection following extraction by these methods. DNA was extracted from all protoscolex samples by all methods, but success for samples with eggs depended on extraction technique with the modified technique showing success on all samples. Following experimental infection of dogs, copro-DNAwas successfully extracted from fecal samples (0.2 g) of dogs in the patent period by all methods. In the prepatent period, PCR testing of feces subsamples (0.2 g) extracted by each technique was positive at a rate of 79.6-94.4%. Extraction by the modified technique with fecal samples of over 1 g showed detection of copro-DNA in all samples in both the patent and prepatent periods, and it produced reproducible detection in the addition recovery test using feces from 72 different domestic dogs. As copro-DNAwas detected for at least 1 day following deworming with administration of anthelmintic drugs in experimentally infected dogs, diagnostic deworming might be useful for clinical examination. Using the present detection method can provide quick and accurate diagnosis of canine E. multilocularis infection, which with prompt management and treatment of infected dogs can prevent pet owners from becoming infected and prevent echinococcosis from spreading into non-endemic areas.
  • Yen Thi Hoang Nguyen, Zhenzhen Wang, Haruhiko Maruyama, Yoichiro Horii, Nariaki Nonaka, Ayako Yoshida
    Journal of Food Safety 37 2 e12301 - e12301 2017年05月 [査読有り][通常論文]
  • Ayman Samir Farid, Eman Mohamed Fath, Shogo Mido, Nariaki Nonaka, Yoichiro Horii
    European Journal of Clinical Investigation 47 3 250 - 261 2017年03月 [査読有り][通常論文]
  • Takao IRIE, Yohei YAMAGUCHI, Pham Ngoc DOANH, Zhi Hong GUO, Shigehisa HABE, Yoichiro HORII, Nariaki NONAKA
    Journal of Veterinary Medical Science 79 8 1419 - 1425 2017年 [査読有り][通常論文]
     
    Infection of boar-hunting dogs with Paragonimus westermani was investigated in Western Japan. Blood and rectal feces were collected from 441 dogs in the three districts (205 in Kinki, 131 in Chugoku and 105 in Shikoku District). In a screening ELISA for serum antibody against P. westermani antigen, 195 dogs (44.2%) showed positive reaction. In the 195 dogs, 8 dogs were found excreting P. westermani eggs after molecular analysis of fecal eggs, and additional 7 were identified serologically for the parasite infection because of their stronger reactivity against P. westermani antigen than against antigens of other species of Paragonimus. A spatial analysis showed that all of the P. westermani infections were found in Kinki and Chugoku Districts. In this area, dogs' experience of being fed with raw boar meat showed high odds ratio (3.35) to the seropositivity in the screening ELISA, and the frequency of such experiences was significantly higher in sero-positive dogs. While clear relationship was not obtained between predation of boars by dogs during hunting and their sero-positivity. Therefore, it is suggested that human activity of feeding with wild boar meat is the risk factor for P. westermani infection in boar-hunting dogs. Considering that hunting dogs could play as a major definitive host and maintain the present distribution of P. westermani in Western Japan, control measures for the infection in hunting dogs, such as prohibition of raw meat feeding and regular deworming, should be undertaken.
  • P.N. Doanh, H.V. Hien, L.A. Tu, N. Nonaka, Y. Horii, Y. Nawa
    Journal of Helminthology 90 6 658 - 662 2016年11月 [査読有り][通常論文]
     
    AbstractConventional identification of Paragonimus species and their natural definitive hosts is based on the morphological features of adult parasites isolated from the lungs of wild mammalian hosts. However, wild animals are protected by strict regulations and sampling is not always possible. Recently, molecular techniques have been developed to identify the internal transcribed spacer (ITS) sequences of Paragonimus eggs in faeces/sputum of human patients. Also, mammalian hosts can be identified using the D-loop sequence of mitochondrial DNA in faecal samples. In this study, we used molecular techniques on faeces from wild animals collected in Da Krong Nature Reserve, Quang Tri province, central Vietnam, where Paragonimus metacercariae are highly prevalent in mountain crabs, to identify Paragonimus species and their natural definitive hosts. The results indicated that wild cats, Prionailurus bengalensis, were infected with at least three different Paragonimus species, P. westermani, P. skrjabini and P. heterotremus. Because all of these species can infect humans in Asian countries, human paragonimiasis should be considered in this area.
  • N. PHAM DOANH, A. LUU TU, T. DUNG BUI, T. HO LOAN, NARIAKI NONAKA, YOICHIRO HORII, DAVID BLAIR, YUKIFUMI NAWA
    Parasitology 143 12 1639 - 1646 2016年10月 [査読有り][通常論文]
     
    SUMMARYParagonimus westermani is one of the most medically important lung flukes and is widely distributed in Asia. It exhibits considerable variation in morphological, genetic and biological features. In central provinces of Vietnam, a high prevalence of metacercariae of this species has been reported from the crab intermediate host, Vietopotamon aluoiense. In this study, we detected P. westermani metacercariae in two additional crab hosts, Donopotamon haii in Quang Tri Province, central Vietnam and Indochinamon tannanti in Yen Bai Province in the north. The latter is a new locality for P. westermani in a northern region of Vietnam where P. heterotremus is the only species currently known to cause human paragonimiasis. Paragonimus westermani metacercariae found in Vietnam showed considerable morphological variation but slight genetic variation based on DNA sequences from the nuclear ribosomal ITS2 region and the mitochondrial 16S gene. Co-infection of the same individual crabs with P. westermani and P. heterotremus and/or some other Paragonimus species was found frequently, suggesting potential for co-infection in humans. The findings of the present study emphasize the need for highly specific molecular and immunodiagnostic methods to differentially diagnose between P. westermani and P. heterotremus infections.
  • 肉用牛におけるトキソカラ属回虫、豚回虫感染の血清学的、分子生物学的検討
    田中 舜, 吉田 彩子, 三澤 尚明, 堀井 洋一郎, 丸山 治彦, 森友 靖生, 野中 成晃
    日本獣医学会学術集会講演要旨集 159回 343 - 343 (公社)日本獣医学会 2016年08月
  • Eimeria zuernii胞子未形成オーシストの形態学的同定基準についての検討
    谷田美和子, 堀井洋一郎, 野中成晃
    獣医寄生虫学会誌 15 1 5 - 11 2016年07月 [査読有り]
  • W. Yamazaki, R. Uemura, S. Sekiguchi, J.-B. Dong, S. Watanabe, Y. Kirino, H. Mekata, N. Nonaka, J. Norimine, M. Sueyoshi, Y. Goto, Y. Horii, M. Kurogi, S. Yoshino, N. Misawa
    Journal of Applied Microbiology 120 6 1711 - 1722 2016年06月 [査読有り][通常論文]
  • Jungo OKAJIMA, Kazuhiko SHIBATA, Eiichi TAKAHASHI, Tsuneyuki NAGAFUCHI, Kazue OKAJIMA, Nariaki NONAKA
    Journal of Veterinary Medical Science 78 5 785 - 790 2016年05月 [査読有り][通常論文]
     
    To elucidate current status of fasciolosis and eurytremosis in beef cattle of Japan, inspection data of Tokyo Metropolitan Shibaura Slaughterhouse where beef cattle were shipped from all over Japan were analyzed, and questionnaire to farmers was conducted to assess the relationship between recognition of the disease occurrence in one's own farm and attention to the diseases. The occurrence of fasciolosis and eurytremosis in beef cattle gradually decreased from 18.6% to 0.06% and from 0.58% to 0.02% during the period of 1964 to 2010, respectively. When the current data from 2009 to 2012 were analyzed, the occurrence of fasciolosis was recognized in cattle produced and fattened all over Japan, indicating the disease was prevalent nationwide. While, 97.5% of Eurytrema infection were detected in cattle produced in Okinawa, Shimane and Kagoshima, indicating the disease was endemic in these regions. Higher occurrence (>0.7%) of fasciolosis was observed in minor breeds, such as Japanese Shorthorn. Japanese Black showed 0.09% and 0.05% of occurrence for fasciolosis and eurytremosis, respectively, but FT crossbred with Japanese Black showed lower occurrence (0.007% and 0.002%, respectively). No tendency of occurrence in the age of cattle at slaughter was recognized, indicating the infections may have occurred at the growing and early fattening stage of cattle. The questionnaire survey revealed that farmers experiencing fasciolosis had more knowledge about the disease, however, factors, such as testing parasite infections and use of anti-Fasciola dewormers, were not affected by the recognition of occurrence.
  • OHARI Yuma, Hiroshi SATO, NONAKA Nariaki, MOHANTA Uday Kumar, HAYASHI Kei, ITAGAKI Tadashi
    Japanese Journal of Veterinary Parasitology 15 2 80 - 83 2016年 [査読有り][通常論文]
  • Takao Irie, Yohei Yamaguchi, Asako Sumen, Shigehisa Habe, Yoichiro Horii, Nariaki Nonaka
    Parasitology Research 114 11 4051 - 4058 2015年11月 [査読有り][通常論文]
     
    Dog feces containing 500 Paragonimus westermani eggs per gram were examined by the Medical General Laboratory (MGL), the simple sedimentation (SS), and the Army Medical School III (AMS III) methods. The number of eggs per gram of feces (EPG) obtained by the MGL method was 17.2 and was significantly lower than those obtained by the SS method (324.0) and the AMS III method (505.6). When isolated P. westermani eggs were processed by the MGL method and four layers (ether, ether-fecal, formalin layers, and sediment) of the final centrifugation product were separately examined, almost 100 % of eggs were found at the ether-fecal layer. Similarly, when fecal samples containing P. westermani, Paragonimus skrjabini miyazakii, Paragonimus ohirai, or Paragonimus harinasutai eggs were processed by the MGL method, more than 95 % of the eggs were found in the supernatant layers. The formalin-ethyl acetate (FEA) method showed a similar tendency as the MGL method and over 90 % of eggs remained in the supernatant layers. Contrary to Paragonimus eggs, 63 and 96 % of Clonorchis and Metagonimus eggs were found in the sediment in the MGL method, respectively. When surfactant (Tween 80) was added to fecal solution, most of Paragonimus eggs spun down in the sediment in the MGL and FEA methods, suggesting that Paragonimus eggs have hydrophobic components on their surface. It is suggested that surfactant addition to the fecal solution should be considered when the MGL method is used for detection of Paragonimus eggs.
  • ヒトの動物由来回虫症診断系を用いた実験感染鶏における抗体検出
    早田 弥生, 吉田 彩子, 丸山 治彦, 三澤 尚明, 堀井 洋一郎, 野中 成晃
    日本獣医学会学術集会講演要旨集 158回 309 - 309 (公社)日本獣医学会 2015年08月
  • リアルタイムPCRによる肉製品からのAscaris suum幼虫の検出(Detection of Ascaris suum larvae existed in meat products by real-time PCR)
    Nguyen Thi Hoang Yen, Wang Zhenzhen, 丸山 治彦, 堀井 洋一郎, 野中 成晃, 吉田 彩子
    日本獣医学会学術集会講演要旨集 158回 309 - 309 2015年08月
  • Y. Kirino, M. Tanida, H. Hasunuma, T. Kato, T. Irie, Y. Horii, N. Nonaka
    Veterinary Record 177 8 202 - 202 2015年08月 [査読有り]
  • 人と食品由来回虫症も感染源対策:牛と家禽の疫学的役割と制御法の探索
    野中成晃
    平成26年度食肉に関する助成研究成果報告書 33 184 - 188 2015年04月01日 [査読無し][通常論文]
  • Hirohisa Mekata, Satoshi Sekiguchi, Satoru Konnai, Yumi Kirino, Kazuyuki Honkawa, Nariaki Nonaka, Yoichiro Horii, Junzo Norimine
    Veterinary Record 176 10 254 - 254 2015年03月 [査読有り][通常論文]
     
    The perinatal transmission of bovine leukaemia virus (BLV) plays a critical role in the spread and persistence of BLV infection in cattle herds. The purpose of this study was to examine the frequency of perinatal infections in an area in Japan and investigate some risk factors associated with infection. Altogether, 129 calves born to BLV-infected cows in a herd in Japan were tested for infection immediately after birth and again at one month of age using nested PCR. Twenty-four calves (18.6 per cent) were infected with BLV, of which 14 (10.8 per cent) and 10 (7.7 per cent) calves were infected via the transplacental and the birth canal routes, respectively. Maternal viral loads, breed, the presence or absence of assistance during parturition and the number of births per dam were evaluated to investigate risk factors associated with infection. Maternal viral load was significantly correlated with the frequency of perinatal infection, and more than 40 per cent of newborn calves born to dams with high viral loads were infected with BLV. The results of this study could contribute towards developing effective eradication programmes by providing necessary data for replacement of breeding cow in the field.
  • 我が国のエキノコックス症と感染源対策
    野中成晃
    獣医疫学雑誌 18 2 150 - 152 2014年12月 [査読無し][招待有り]
  • Hideharu Tsukada, Wei Li, Hong Duo, Zhihong Guo, Yong Fu, Mao Peng, Xiuying Shen, Jianwu Jing, Aishan Yuan, Ma Ni, Shengde He, Fuqiang Huang, Kai Feng, Keisuke Ishikawa, Ikuo Kobayashi, Akio Shinohara, Nariaki Nonaka
    WILDLIFE BIOLOGY 20 6 356 - 361 2014年12月 [査読有り][通常論文]
     
    We compared the diet and the spatial distribution of the Tibetan fox Vulpes ferrilata and the red fox Vulpes vulpes in the Tibetan plateau, to elucidate mechanisms of coexistence for these two sympatric canids and to clarify their roles as definitive hosts for zoonotic Echinococcus parasites. Diet and fine-scale distribution patterns were assessed by fecal DNA analysis. A total of 45 fecal samples (15 belonging to Tibetan fox, 30 belonging to red fox were collected from 15 sites into three of which contained only Tibetan fox feces, six only red fox feces, and six contained feces of both species. The abundance of pika burrows, a key prey item for both species, did not differ among the sites. Food composition analysis, estimated using a point-frame method, revealed slight but insignificant differences between the two species. Tibetan foxes consumed primarily mammals, whereas red foxes consumed primarily insects. The dietary range of the Tibetan fox was narrower than that of the red fox but there was little dietary overlap between the two species. These findings suggest that the weak partitioning of food resources between Tibetan and red foxes can facilitate their coexistence even within the same habitat where they share the same key prey items, i.e. small mammals such as pikas. These dietary differences between the two fox species also suggest that the Tibetan fox is a more important definitive host for Echinococcus on the Tibetan plateau than is the red fox.
  • 鶏における犬・猫・豚回虫感染検査法の検討
    早田 弥生, 吉田 彩子, 丸山 治彦, 三澤 尚明, 堀井 洋一郎, 野中 成晃
    日本獣医学会学術集会講演要旨集 157回 367 - 367 (公社)日本獣医学会 2014年08月
  • 黒毛和種子牛における溷晴虫症の一例
    林淳, 郭志宏, 野中成晃, 永延清和
    家畜診療 61 3 165 - 168 2014年03月 [査読有り][通常論文]
  • Wei LI, Yong FU, Hong DUO, Zhihong GUO, Xiuying SHEN, Fuqiang HUANG, Kai FENG, Zhisheng DANG, Peng MAO, Fang WANG, Tetsuo NASU, Nariaki NONAKA
    Journal of Veterinary Medical Science 76 2 183 - 188 2014年 [査読有り][通常論文]
     
    The prevalence of Hypoderma spp. in yaks grazed in the east of Qinghai province was investigated in 2008. In this area, the prevalence in young yaks (1- to 3-year-old) was very high at 82.2-98.7%, whilst in adult yaks (4-year-old and older), the prevalence was 42.4-50.6%. The seasonal development and migration pattern of Hypoderma larvae in yak bodies was found to be similar for different locations in this area. The numbers of first, second and third instar larvae detected in yak bodies peaked in October, December and March, respectively. Different doses of ivermectin (125 to 500 mu g/kg body weight) almost completely dewormed the larvae from yaks, suggesting that using a quarter of the prescribed dose (500 mu g/kg body weight) was effective. In October of each year between 2009 and 2012, ivermectin (125 mu g/kg body weight) was administered to a total of 562,995 yaks grazed in four counties in Qinghai province, and the pevalence of Hypoderma larval infection in yaks was reduced to 0.5-1.0%.
  • Zhihong GUO, Wei LI, Mao PENG, Hong DUO, Xiuying SHEN, Yong FU, Takao IRIE, Tiantian GAN, Yumi KIRINO, Tetsuo NASU, Yoichiro HORII, Nariaki NONAKA
    Journal of Veterinary Medical Science 76 3 395 - 400 2014年 [査読有り][通常論文]
     
    An epidemiological study and control trial were conducted to assess taeniid infection in farm dogs in Qinghai Province, China. To improve egg detection by fecal examination, a deworming step with praziquantel was incorporated into the sampling methodology. As a result, a marked increase in the number of egg-positive samples was observed in samples collected at 24 hr after deworming. Then, the fecal examination and barcoding of egg DNA were performed to assess the prevalence of taeniid species in dogs from Xinghai, Haiyan, Gangcha and Chengduo counties. Analysis of 277 dog feces revealed that taeniid cestodes, including Taenia spp. and Echinococcus granulosus, were highly prevalent in Xinghai (34.4%), but eggs were not found in Haiyan where a control trial on canine echinococcosis had been conducted 20 years previously. A control trial involving the administration of 5-10 mg/kg praziquantel to 90 farm dogs at 45-day intervals was conducted in Xinghai. The prevalence of taeniid cestodes in the dogs was reduced to 9.6% and 4.9% after one and two years, respectively, indicating that some dogs were not administered praziquantel properly. A questionnaire survey of farmers in Xinghai and Haiyan revealed that most farmers in Xinghai were not familiar with echinococcosis or the transmission route of the disease, while most farmers in Haiyan had a more thorough understanding of the disease. The findings implied that a program for educating local farmers would be important for efficiently controlling canine taeniid infection in the region.
  • Takako Ikeda, Masashi Yoshimura, Keiichi Onoyama, Yuzaburo Oku, Nariaki Nonaka, Ken Katakura
    Parasites & Vectors 7 1 357 - 357 2014年 [査読有り][通常論文]
     
    Background: Deworming wild foxes by baiting with the anthelmintic praziquantel is being established as a preventive technique against environmental contamination with Echinococcus multilocularis eggs. Improvement of the cost-benefit performance of baiting treatment is required urgently to raise and maintain the efficacy of deworming. We established a spatial model of den site selection by urban red foxes, the definitive host, to specify the optimal micro-habitats for delivering baits in a new modeling approach modified for urban fox populations. Methods: The model was established for two cities (Obihiro and Sapporo) in Hokkaido, Japan, in which a sylvatic cycle of E. multilocularis is maintained. The two cities have different degrees of urbanization. The modeling process was designed to detect the best combination of key environmental factors and spatial scale that foxes pay attention to most (here named 'heeding range') when they select den sites. All possible models were generated using logistic regression analysis, with "presence" or "absence" of fox den as the objective variable, and nine landscape categories customized for urban environments as predictor variables to detect the best subset of predictors. This procedure was conducted for each of ten sizes of concentric circles from dens and control points to detect the best circle size. Out of all models generated, the most parsimonious model was selected using Akaike's Information Criterion (AIC) inspection. Results: Our models suggest that fox dens in Obihiro are located at the center of a circle with 500 m radius including low percentages of wide roads, narrow roads, and occupied buildings, but high percentages of green covered areas; the dens in Sapporo within 300 m radius with low percentages of wide roads, occupied buildings, but high percentages of riverbeds and green covered areas. The variation of the models suggests the necessity of accumulating models for various types of cities in order to reveal the patterns of the model. Conclusions: Our denning models indicating suitable sites for delivering baits will improve the cost-benefit performance of the campaign. Our modeling protocol is suitable for the urban landscapes, and for extracting the heeding range when they select the den sites.
  • P.N. Doanh, H.V. Hien, N. Nonaka, Y. Horii, Y. Nawa
    Journal of Helminthology 87 4 450 - 456 2013年12月 [査読有り][通常論文]
     
    AbstractTwo members of the Paragonimus skrjabini complex, P. skrjabini and P. miyazakii, are now considered as two sub-species, P. skrjabini skrjabini and P. skrjabini miyazakii. They are well known as important pathogens for human paragonimiasis in China and Japan. Recently, members of this species complex have been reported from India. Here we report the first discovery of P. skrjabini from freshwater crab hosts in Thanh Hoa province, Vietnam. For morphological and molecular phylogenetic studies, adult worms were obtained by experimental infection in cats and dogs. Molecular analyses of metacercariae and adults revealed that the P. skrjabini population from Thanh Hoa, Vietnam was almost completely identical with that from Yunnan province, China. Those populations from Thanh Hoa, Vietnam and Yunnan, China and those from Manipur, India were significantly different from P. skrjabini populations reported from other localities of China in cytochrome oxidase subunit 1 (CO1) gene sequences, indicating considerable genetic variation within the P. skrjabini complex. Moreover, low bootstrap values in the CO1 tree suggested that more variant genotypes belonging to P. skrjabini complex may be found in other Asian countries in between Vietnam and India, such as Myanmar, Laos and Thailand. Since P. skrjabini is known as a pathogen for humans, paragonimiasis cases caused by P. skrjabini might be found in Vietnam and other Asian countries.
  • Ayman Samir Farid, Kazuyuki Honkawa, Eman Mohamed Fath, Nariaki Nonaka, Yoichiro Horii
    BMC Veterinary Research 9 1 2013年12月 [査読有り][通常論文]
     
    Abstract Background Fatty liver is a major metabolic disorder in dairy cows and is believed to result in major economic losses in dairy farming due to decreased health status, reproductive performance and fertility. Currently, the definitive means for diagnosing fatty liver is determining the fat content of hepatic tissue by liver biopsy, which is an invasive and costly procedure, making it poorly suited to dairy farms. Therefore, the key aim of this study was to investigate the measurement of serum paraoxonase-1 (PON1), an enzyme exclusively synthesized by the liver, as a sensitive noninvasive biomarker for diagnosis of fatty liver in dairy cows. Results A comparative cohort study using serum specimens from Holstein–Friesian dairy cows (46 healthy and 46 fatty liver cases) was conducted. Serum PON1 (paraoxonase, lactonase and arylesterase) activity and other biochemical and hematological parameters were measured. We found that serum PON1 activity was lower (P<0.001) in cows suffering from fatty liver. The area under the receiver operating characteristic curve (AUC-ROC) of PON1 activity for diagnosis of fatty liver was 0.973–0.989 [95% confidence interval (CI) 0.941, 1.000] which was higher than the AUC-ROC of aspartate aminotransferase (AST), lecithin-cholesterol acyltransferase (LCAT), alkaline phosphatase (ALP), non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), total cholesterol, high-density lipoprotein (HDL) and low-density lipoprotein (LDL). We found that adding serum PON1 measurement to different batteries of serum diagnostic panels showed a combination of high sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (+LR), negative likelihood ratio (−LR), diagnostic odd ratio (DOR) and overall diagnostic accuracy in diagnosing fatty liver. Conclusions The present results indicate that addition of serum PON1 activity measurement to the biochemical profile could improve the diagnosis of fatty liver in dairy cows, which would have a considerable clinical impact and lead to greater profitability in the dairy industry.
  • Li W, Guo Z, Duo H, Fu Y, Peng M, Shen X, Tsukada H, Irie T, Nasu T, Horii Y, Nonaka N
    The Journal of veterinary medical science 75 10 1329 - 1333 2013年10月 [査読有り][通常論文]
  • Pham Ngoc Doanh, Zhihong Guo, Nariaki Nonaka, Yoichiro Horii, Yukifumi Nawa
    Parasitology International 62 3 240 - 245 2013年06月 [査読有り][通常論文]
  • P.N. Doanh, H.V. Hien, N. Nonaka, Y. Horii, Y. Nawa
    Journal of Helminthology 87 2 141 - 146 2013年06月 [査読有り][通常論文]
     
    AbstractAmong about 50 nominal Paragonimus species, Paragonimus proliferus is rather a rare species, found only in Yunnan province, China, until our recent discovery of this species in Lai Chau province, northern Vietnam close to Yunnan, China. Here we add Quang Binh province, central Vietnam as a new endemic area of P. proliferus. Large excysted metacercariae found in mountainous crabs, Potamiscus tannanti, were morphologically identified as P. proliferus, which was confirmed further by molecular analyses. Second internal transcribed spacer (ITS2) sequences of the P. proliferus population in Quang Binh province were completely (100%) identical with those of P. proliferus populations in Lai Chau province, northern Vietnam and Yunnan province, China. However, cytochrome oxidase subunit 1 (CO1) gene sequences of Quang Binh population were significantly different (5.6%) from that of previously reported northern Vietnam and Yunnan, China populations. A phylogenetic tree revealed that all CO1 sequences of P. proliferus Quang Binh population formed a distinct group, which was clustered with northern Vietnam and Yunnan, China populations with the bootstrap value of 75%. This is the first record of the genetically variant population of P. proliferus, distribution of which is geographically remote from the previously reported endemic areas in the border between northern Vietnam and Yunnan, China, suggesting that P. proliferus may be much more widely distributed in the Indochina peninsula (or South-East Asia) than expected.
  • 牛コクシジウム症コントロールの重要性とその背景
    桐野有美, 野中成晃, 堀井洋一郎
    家畜診療 60 6 343 - 350 2013年06月 [査読有り][通常論文]
  • 講座:食品における寄生動物感染症11,蠕虫感染症(6)顎口虫
    野中成晃
    日本防菌防黴学会誌 41 6 339 - 342 2013年06月 [査読無し][招待有り]
  • Takao IRIE, Kohei SAKAGUCHI, Aino OTA-TOMITA, Miwako TANIDA, Kanako HIDAKA, Yumi KIRINO, Nariaki NONAKA, Yoichiro HORII
    Journal of Veterinary Medical Science 75 12 1585 - 1589 2013年 [査読有り][通常論文]
     
    Infection with Moniezia benedeni is sometimes found in confined cattle in Japan. Between October 2011 and January 2013, we monitored the fecal egg prevalence at a confined cattle farm in Miyazaki prefecture where continuous M benedeni infection has been recognized for years to evaluate the possible infection routes. Fecal egg prevalence changed seasonally with the highest in October 2011 (27.3%: 9/33). This was followed by a gradual decrease until July 2012 (9.4%: 3/32) and then an increase between August to December 2012 when new egg-excreting cases were observed. The pattern of seasonal changes was similar to that reported previously for cattle kept in a barn with an outside playing yard. Although M benedeni-infected mites were not found, we constantly detected an oribatid mite, Oribatula sakamorii Aoki, 1970, in the litter of cattle bedding from May to October 2012. This species belongs to a genus which has been reported to be a suitable intermediate host for M benedeni, suggesting that M benedeni infection may have been autonomously maintained at the farm via oribatid mites living in the cowshed. When infected cattle were treated with praziquantel, it was found that a single oral inoculation with a dose of 5 mg/kg was effective for deworming.
  • Survey and molecular identification of Echinococcus species in Tibetan dogs and wild foxes in Qinghai Province.
    Duo, H, Diao, X.-L, Fu, Y, Shen, X.-Y, Peng, M, Zhao, H.-L, Nonaka, N, Guo, Z.-H, Genqiu, J, Ni, M, He, S.-D, Chang, M.-H, Huang, F.-Q, Feng, K, Li, W, Wu, R
    Acta Parasitology et Medica Entomologica Sinica 19 4 220 - 227 2012年12月 [査読有り][通常論文]
  • Pham Ngoc Doanh, Hoang Van Hien, Nariaki Nonaka, Yoichiro Horii, Yukifumi Nawa
    Parasitology International 61 3 399 - 404 2012年09月 [査読有り][通常論文]
     
    During our epidemiological surveys for Paragonimus species in central Viet Nam, we found four morphologically different Paragonimus metacercariae in mountainous crabs. They were identified as metacercariae of Paragonimus westermani, P. bangkokensis, P. proliferus, and P. harinasutai in the order of their prevalence in crab hosts. This is the first discovery of P. harinasutai in Viet Nam, co-inhabiting with P. bangkokensis and other species. Metacercariae of P. harinasutai were given orally to a cat to obtain adult worms. Then, ITS2 and CO1 sequences of metacercariae and adults of P. harinasutai, and metacercariae of P. bangkokensis collected from the same place were determined for analyses of phylogenetic relationships to other P. harinasutai and P. bangkokensis populations as well as related species. The results of molecular analyses showed that P. harinasutai from Quang Binh province of central Viet Nam was almost completely identical with those from Vientiane, Lao PDR P. bangkokensis from Quang Binh, Viet Nam was also almost completely identical with those from Lao PDR and from Quang Ninh province, Viet Nam. Except for one P. harinasutai isolate from China, all populations of P. harinasutai and P. bangkokensis from Thailand, Lao and Viet Nam make a single clade in both ITS2 and CO1 trees. In ITS2 sequences, AT deletion and ATC insertion were observed in some isolates of both species, indicating recent gene flow between P. harinasutai and P. bangkokensis. Moreover, because of their extremely high genetic similarities and their co-inhabitation in the same crab hosts found in Thailand, Lao PDR and Viet Nam, they should be considered as the sister species at the early stage of divergence. In addition, P. microrchis previously described from Yunnan, China should be placed as the synonym of P. harinasutai, because of their morphological and molecular similarities. © 2012 Elsevier Ireland Ltd.
  • CT検査による肺吸虫のラット体内動態の経時的観察
    小西 祐子, 入江 隆夫, 佐藤 裕之, 野中 成晃, 堀井 洋一郎, 浅沼 武敏
    日本獣医学会学術集会講演要旨集 154回 207 - 207 (公社)日本獣医学会 2012年08月
  • Saruda Tiwananthagorn, Abdul Manan Bhutto, Javed Hussain Baloch, Farooq Rahman Soomro, Yuta Kawamura, Ryo Nakao, Keisuke Aoshima, Nariaki Nonaka, Yuzaburo Oku, Ken Katakura
    Parasitology Research 111 1 125 - 133 2012年07月 [査読有り][通常論文]
     
    Leishmania (Leishmania) major has been identified as the major causative agent of cutaneous leishmaniasis in Sindh Province of southern Pakistan. To make a rational approach for understanding the pathogen transmission cycles, the sand fly species and their natural blood meals in the endemic areas were examined. Total DNA was individually extracted from sand flies collected in four villages in Sindh Province. PCR-RFLP (restriction fragment length polymorphism) and sequence analysis of the 18S ribosomal RNA gene revealed that female sand flies identified were Sergentomyia clydei/Sergentomyia ghesquierei/Sergentomyia magna (68.6%), Sergentomyia dubia (17.1%), Phlebotomus papatasi (7.4%), Phlebotomus alexandri-like sand flies (3.4%) and Sergentomyia dentata (3.4%). PCR amplification of leishmanial kinetoplast DNA did not result in positive signals, suggesting that all 175 tested female sand flies were not infected with leishmanial parasites or contained undetectable levels of leishmanial DNA. Amplification and sequencing of the vertebrate cytochrome b gene in 28 blood-fed sand flies revealed that P. papatasi fed on cattle and wild rat whereas P. alexandri-like specimens fed on human, cattle, goat and dog. Although Sergentomyia sand flies are generally known to feed on cold-blooded animals, S. clydei, S. dubia and S. ghesquierei preferred humans, cattle, goat, sheep, buffalo, dog, donkey, wild rat and Indian gerbil. The epidemiological significance of the zoophilic feeding on various host species by Phlebotomus and Sergentomyia sand flies in Pakistan is further required to study for better understanding the zoonotic transmission of sand-fly-borne pathogens and for appropriate management of the vectors.
  • Shogo Mido, Eman Mohamed Fath, Ayman Samir Farid, Nariaki Nonaka, Yuzaburo Oku, Yoichiro Horii
    Experimental Parasitology 131 2 190 - 194 2012年06月 [査読有り][通常論文]
     
    Paraoxonase-1 (PON1) is an HDL-associated enzyme with anti-atherogenic properties. Reduced PON1 activity has previously been observed in Nippostrongylus brasiliensis-infected rats. However, the effect of chronic zoonotic nematode infections on serum PON1 activity has not yet been studied. Therefore, we evaluated the effect of Trichinella spiralis infection on serum PON1 activity, the lipid profile, and oxidative stress in rats. There were significant reductions in serum PON1 activities (Day 2-Week 7 post-infection) in rats infected with T. spiralis, and these reductions were associated with significant increases in the serum levels of triglyceride and LDL/VLDL, as well as a significant reduction in the level of HDL. Moreover, T. spiralis infection was associated with a status of oxidative stress indicated by increased concentrations of superoxide dismutase and malondialdehyde. Given the zoonotic prevalence of T. spiralis and the cardioprotective role of PON1, further mechanistic research in this area is warranted. (C) 2012 Elsevier Inc. All rights reserved.
  • Zhi Hong Guo, Meiko Kubo, Momotoshi Kudo, Kazumi Nibe, Yoichiro Horii, Nariaki Nonaka
    Parasitology International 60 4 498 - 502 2011年12月 [査読有り][通常論文]
     
    At the abattoir on study in Miyazaki. Japan, 9537 imported cattle from Australia in average were slaughtered annually in the last 5 years (2006 to 2010) and hydatid cysts were constantly detected in about 1.8% of the cattle. In order to assess the risk of Echinococcus granulosus delivered to Japan by imported cattle, 250 cysts found in 103 cattle at the abattoir were examined for their biological characteristics and genotypes. The cattle slaughtered were imported from Australia at an age of 10-12 months old and fattened for 17-18 months in Japan. The cysts showed their size ranging from 4 to 108 mm and were mainly found in the lung. Mature protoscoleces were detected in the three largest cysts, all were of the G1 genotype. Most of the other cysts contained clear cyst fluid and had thin laminated layer with no protoscoleces. The finding implies a potential risk of E. granulosus being established in Japan, thus strict and proper meat inspection and consequent offal condemnation are requisite at abattoirs that deal with imported cattle. Genotyping based on partial fragments of mitochondrial cox1, rmS and nad1 genes were performed on the 66 cysts, showing that most of the cysts were Cl genotype (common sheep strain). However, two and four cysts were considered as G2 (Tasmanian sheep strain) and G3 (buffalo strain) genotypes, respectively. Since it has been widely recognized that Cl is the only genotype distributing in mainland Australia and that G2 genotype has been eradicated from Tasmania, the finding of those genotypes from Australian cattle indicated that certain genotypes other than G1 genotype are distributing in mainland Australia. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
  • Coprological survey of alimentary tract parasites in dogs from Zambia and evaluation of a coproantigen assay for canine echinococcosis
    Nonaka, N, Nakamura, S, Inoue, T, Oku, Y, Katakura, K, Matsumoto, J, Mathis, A, Chembesofu, M, Phiri, I. G. K
    Annals of Tropical Medicine and Parasitology 105 7 521 - 530 2011年10月 [査読有り][通常論文]
  • 経時的CT検査による肺吸虫のイヌ体内動態の観察
    小川 篤史, 佐藤 裕之, 三堂 祥吾, 野中 成晃, 山口 洋平, 入江 隆夫, 浅沼 武敏, 堀井 洋一郎
    獣医寄生虫学会誌 9 2 133 - 133 日本獣医寄生虫学会 2011年03月
  • Maria Teresa Armua-Fernandez, Nariaki Nonaka, Tatsuya Sakurai, Seita Nakamura, Bruno Gottstein, Peter Deplazes, Isaac G.K. Phiri, Ken Katakura, Yuzaburo Oku
    Parasitology International 60 1 84 - 89 2011年01月 [査読有り][通常論文]
     
    We report the development of a colourimetric PCR/dot blot assay targeting the mitochondrial gene NADH dehydrogenase subunit 1 (nad1) for differential diagnosis of taeniid eggs. Partial sequences of the cestode nad1 gene were aligned and new primers were designed based on conserved regions. Species-specific oligonucleotide probes (S-SONP) for canine taeniid cestodes were then designed manually based on the variable region between the conserved primers. Specifically, S-SONP were designed for the Taenia crassiceps, T. hydatigena, T. multiceps, T. ovis, T. taeniaeformis, Echinococcus granulosus (genotype 1), E. multilocularis and E. vogeli. Each probe showed high specificity as no cross-hybridisation with any amplified nad1 fragment was observed. We evaluated the assay using 49 taeniid egg-positive samples collected from dogs in Zambia. DNA from 5 to 10 eggs was extracted in each sample. Using the PCR/dot blot assay, the probes successfully detected PCR products from T. hydatigena in 42 samples, T. multiceps in 3 samples, and both species (mixed infection) in the remaining 4 samples. The results indicate that the PCR/dot blot assay is a reliable alternative for differential diagnosis of taeniid eggs in faecal samples. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
  • 経時的CT検査による肺吸虫のイヌ体内動態の観察
    小川 篤史, 佐藤 裕之, 三堂 祥吾, 野中 成晃, 山口 洋平, 入江 隆夫, 浅沼 武敏, 堀井 洋一郎
    日本獣医学会学術集会講演要旨集 150回 183 - 183 (公社)日本獣医学会 2010年09月
  • A. J Hulsmeier, P. Deplazes, S. Naem, N. Nonaka, T. Hennet, P. Kohler
    Glycobiology 20 1 127 - 135 2010年01月01日 [査読有り][通常論文]
     
    A major surface constituent of Echinococcus multilocularis adult worms, referred to as an EmA9 antigen, was immunoaffinity purified and identified as a high-molecular-weight glycoconjugate. Labeling studies using the monoclonal antibody MAbEmA9 indicated that this antigen undergoes a regulated expression during the development from the larval to the adult parasite. Chemical modification of carbohydrate by periodate oxidation resulted in a reduced reactivity with antigen-specific antibodies. Non-reductive β-elimination of the purified molecule indicated the presence of O-linked glycans attached to threonine residues. Carbohydrate compositional analyses indicated the presence of N- and O-glycans with the ratio of carbohydrate to protein being 1.5:1 (w/w). N- and O-linked glycans were released by hydrazinolysis and analyzed as 2-aminobenzamide derivatized glycans by mass spectrometry together with HPLC and enzymatic sequencing. Novel linear O-linked saccharides with multiple β-HexNAc extensions of reducing end Gal were identified. N-Linked glycans were also detected with oligomannose and mono-, bi-, tri- and tetra-antennary-type structures, most of which were found to be core-fucosylated. Taken together, the results indicate that the EmA9 antigen is a glycoprotein located at the outer surface of the adult E. multilocularis. The observation that the EmA9 antigen expression is developmentally regulated suggests an involvement of this glycoprotein in the establishment of the parasite in its canine host. © The Author 2009. Published by Oxford University Press. All rights reserved.
  • Nariaki Nonaka, Takafumi Sano, Takashi Inoue, Maria Teresa Armua, Daisuke Fukui, Ken Katakura, Yuzaburo Oku
    Parasitology Research 106 1 75 - 83 2009年12月 [査読有り][通常論文]
  • Jose Trinipil G. Lagapa, Yuzaburo Oku, Masami Kaneko, Sumiya Ganzorig, Takashi Ono, Nariaki Nonaka, Fumio Kobayashi, Masao Kamiya
    Environmental Health and Preventive Medicine 14 5 299 - 303 2009年09月 [査読有り][通常論文]
     
    Objectives The aim of this study was to determine the prevalence of Echinococcus multilocularis environmental contamination in an urban fringethe Nopporo forest park of Sapporo city, Hokkaido, Japan. A secondary aim was to determine possible transmission risks areas by associating percentage occurrence of E. multilocularis-positive faeces with the different land-use classes. Methods Wild fox faeces collected from the environment were examined by intravital methods, such as the taeniid egg sucrose floatation technique, E. multilocularis coproantigen enzyme-linked immunosorbent analysis and DNA test of taeniid eggs by PCR. Geospatial maps produced by the Global Positioning System and Landsat data were analysed using geographic information system software to determine the association between percentage occurrences of E. multilocularis- positive fox faeces and land-use classes. Results Our findings showed high prevalence rates in both E. multilocularis egg and coproantigen-positive faeces (16 and 49%, respectively) in the investigated urban fringe forest park. Data revealed that percentage occurrence of E. multilocularis-positive fox faeces was associated with land-use classes, such as forest and open field (P< 0.05). Conclusions We conclude that Nopporo forest park in the urban fringe of Sapporo city, Hokkaido is a reservoir with a high prevalence of zoonotic infective agents for alveolar echinococcosis. Our findings suggest that interface habitats between forests or woodlands and open fields are indispensable for continued maintenance of the life-cycle of E. multilocularis and, as such, constitute high risk areas for echinococcosis transmission. © 2009 The Japanese Society for Hygiene.
  • Nonaka N, Kamiya M, Kobayashi F, Ganzorig S, Ando S, Yagi K, Iwaki T, Inoue T, Oku Y
    Vector borne and zoonotic diseases (Larchmont, N.Y.) 9 2 201 - 206 2009年04月 [査読有り][通常論文]
  • Prevalencia de Echinococcus granulosus en Uruguay
    Elola, S, Sakai, H, Armua, T, Nonaka, N, Oku, Y, Irabedra, P, Valledor, S, Cabrera, P
    Zoonosis 1 1 34 - 35 2009年 [査読有り][通常論文]
  • Nakao, R, Mizukami, C, Kawamura, Y, Subeki Bawn, S, Yamasaki, M, Maede, Y, Matsuura, H, Nabeta, K, Nonaka, N, Oku, Y, Katakura, K
    Journal of Veterinary Medical Science 71 1 33 - 41 2009年01月 [査読有り][通常論文]
     
    Bruceine A, a natural quassinoid compound extracted from the dried fruits of Brucea javanica (L.) Merr., was evaluated for its antibabesial activity in vitro and in vivo. Bruceine A inhibited the in vitro growth of Babesia gibsoni in canine erythrocytes at lower concentration compared with the standard antibabesial drug diminazene aceturate and killed the parasites within 24 hr at a concentration of 25 nM. Oral administration of bruceine A at a dosage of 6.4 mg/kg/day for 5 days resulted in no clinical findings in a dog with normal ranges of hematological and biochemical values in the blood. Three dogs were infected with B. gibsoni and two of them were treated with bruceine A at a dosage of 6.4 mg/kg/day for 6 days from day 5 post-infection. An untreated dog developed typical acute babesiosis symptoms including severe anemia, high fever, and complete loss of appetite and movement. However, the two bruceine A-treated dogs maintained their healthy conditions throughout the experimental period of 4 weeks although complete elimination of parasites from the peripheral blood was not achieved and decreases in the packed cell volume and the erythrocyte and platelet counts were observed. Since natural quassinoid compounds have been used as traditional medicines for the treatment of various ailments including cancer and malaria, the present results suggest that bruceine A or other related compounds are potential candidates for the treatment of canine babesiosis.
  • Nonaka, N, Kamiya, M, Oku, Y
    Journal of Veterinary Medical Science 71 1 105 - 107 2009年01月 [査読有り][通常論文]
     
    A questionnaire survey was conducted by giving 14 statements about echinococcosis to 2,070 dog owners residing in Hokkaido in order to evaluate their understanding about the biology and epidemiology of Echinococcus multilocularis. Analysis of the answers revealed that dog owners understood the disease superficially, and there were several points of confusion in their understanding, especially regarding differences in the modes of transmission and disease development in dogs and humans. The results suggest the need for the proper education of dog owners to perform proper prophylactic measures against the disease.
  • Saw Bawm, Hideyuki Matsuura, Ahmed Elkhateeb, Kensuke Nabeta, Subeki, Nariaki Nonaka, Yuzaburo Oku, Ken Katakura
    Veterinary Parasitology 158 4 288 - 294 2008年12月 [査読有り][通常論文]
     
    The medicinal plant Brucea javanica (L.) Merr. (Simaroubaceae) is widely distributed throughout Asia where its bitter fruits have been used in traditional medicine for various ailments. Fifteen C-20 quassinoids were isolated from the fruits of B. javanica and examined for their in vitro antitrypanosomal activities against trypomastigotes of Trypanosoma evansi. Bruceine A, bruceantinol, bruceine C, brusatol, and bruceine B showed strong antitrypanosomal activities with IC50 values in the range of 2.9-17.8 nM, which compared well with the standard trypanocidal drugs diminazene aceturate (IC50 = 8.8 nM) and suramin (IC50 = 43.2 nM). However, dehydrobruceine A, dehydrobruceine B, and dehydrobrusatol were about 2100, 900, and 1200 times less active, respectively, than bruceine A, bruceine B, and brusatol. The relationship of the structure and antitrypanosomal activity of these quassinoid compounds suggested that the presence of a diosphenol moiety in ring A and the nature of the C-15 side chain are important for their activities against T. evansi. This is the first report on the antitrypanosomal activity of isolated quassinoids. © 2008 Elsevier B.V. All rights reserved.
  • Nonaka N, Hirokawa H, Inoue T, Nakao R, Ganzorig S, Kobayashi F, Inagaki M, Egoshi K, Kamiya M, Oku Y
    Parasitology international 57 4 519 - 520 4 2008年12月 [査読有り][通常論文]
  • Nonaka, N, Oka, M, Kamiya, M, Oku, Y
    Veterinary Parasitology 152 3-4 278 - 283 2008年04月 [査読有り][通常論文]
     
    A latex agglutination test for detecting Echinococcus multilocularis coproantigen in definitive hosts was developed using latex beads sensitized with EmA9 monoclonal antibody raised against somatic antigens of adult E. multilocularis. A primary test (LA 1) was performed on 82 fecal samples of necropsied foxes, of which 46 were infected, and resulted in 61% sensitivity and 86% specificity. To increase the sensitivity, 4 ng/mL of excretory/secretory antigens of adult worms was added to the samples in a secondary test (LA 2), resulting in 91% sensitivity and 61% specificity. The positive predictive value of the LA 1 test and the negative predictive value of the LA 2 test were both 85%. The combination of the LA 1 and LA 2 tests is applicable and practical for use in situations that require quick diagnosis or screening based on the following interpretation: the samples that are positive in the LA 1 test are positive; the samples that are negative in the LA 2 test are negative; and the samples that are negative in the LA 1 test and positive in the LA 2 test are classified as suspicious. (C) 2008 Elsevier B.V. All rights reserved.
  • Lagapa, J. T, Oku, Y, Nonaka, N, Kamiya, M
    Experimental Parasitology 118 4 576 - 582 2008年04月 [査読有り][通常論文]
     
    Fate and proliferation of gastric mucosal cells during hyperplasia of Taenia taeniaeformis eggs inoculated Wistar rats were investigated using PCNA immunohistochemistry, BrdU labeling and other histopathologic staining techniques. Results revealed marked cell proliferation in gastric corpus and antral mucosa of infected rats as evidenced by increased lengths of proliferative zones and indices of BrdU labeling. The gastropathy in corpus was characterized by massive accumulation of precursors, neck and intermediate cells following significant decreases in numbers of parietal and zymogenic cells. Gastropathy in antrum was described with significant increases in precursors and mucous cells. Our results suggested that T taeniaeformis-induced gastric hyperplasia was initiated by depletion of parietal cells presumably due to the cestode's ES products. As a result, there was inhibition of zymogenic cell differentiation due to the disruption of normal development pathways of gastric mucosal lineages. These sequences of events were considered to cause the increase in cell proliferation and accumulation of intermediate cells resulting to the hyperplastic lesions. (c) 2007 Elsevier Inc. All rights reserved.
  • Matsumoto Jun, Sakamoto Kimitoshi, Shinjyo Noriko, Kido Yasutoshi, Yamamoto Nao, Yagi Kinpei, Miyoshi Hideto, Nonaka Nariaki, Katakura Ken, Kita Kiyoshi, Oku Yuzaburo
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 52 1 164 - 170 2008年01月 [査読有り][通常論文]
  • Mitochondrial DNA phylogeography of the red fox (Vulpes vulpes) in northern Japan
    Inoue, T, Nonaka, N, Mizuno, A, Morishima, Y, Sato, H, Katakura, K, Oku, Y
    Zoological Science 24 12 1178 - 1186 2007年12月 [査読有り][通常論文]
  • Molecular mechanisms involved in the growth and development of Echinococcus multilocularis metacestodes
    Matsumoto, J, Nonaka, N, Katakura, K, Oku, Y
    Japanese Journal of Veterinary Parasitology 6 1 7 - 12 2007年11月 [査読有り][通常論文]
  • Inoue T, Nonaka N, Kanai Y, Iwaki T, Kamiya M, Oku Y
    Veterinary parasitology 150 1-2 88 - 96 2007年11月 [査読有り][通常論文]
  • 地域住民による北海道のエキノコックス感染源対策の試行
    奥 祐三郎, 巖城 隆, スミヤ・ガンゾリック, 野中 成晃, 宮原 俊之, 岡崎 克則, 神谷 正男
    獣医寄生虫学会誌 5 2 36 - 36 日本獣医寄生虫学会 2007年03月 [査読無し][通常論文]
  • Echinococcosis risk among domestic definitive hosts, Japan
    Kamiya, M, Lagapa, J. T, Ganzorig, S, Kobayashi, F, Nonaka, N, Oku, Y
    Emerging Infectious Diseases 13 2 346 - 347 2007年02月 [査読有り][通常論文]
  • Kanai, Y, Inoue, T, Mano, T, Nonaka, N, Katakura, K, Oku, Y
    Japanese Journal of Veterinary Research 54 4 175 - 182 2007年02月 [査読有り][通常論文]
     
    In order to evaluate the present epidemiological situation of Trichinella infection in wild animals in Hokkaido, Japan, red foxes (Vulpes vulpes), raccoon dogs (Nyctereutes procyonoides) , brown bears (Ursus arctos) , martens (Martes melampus), rodents and insectivores captured in Hokkaido were examined for muscle larvae by the artificial digestion method from 2000 to 2006. Foxes (44/319, 13.8%), raccoon dogs (6/77, 7.8%) and brown bears (4/126, 3.2%) were found to be infected with Trichinella larvae and all other animal species evaluated were negative. Multiplex PCR and DNA sequencing revealed that larvae from a fox captured in Otofuke, in south-eastern Hokkaido, were T. nativa, and larvae from 27 animals including 21 foxes, 2 raccoon dogs and 4 brown bears captured in western Hokkaido were Trichinella T9.
  • 北海道のエキノコックス感染源対策の試みと今後の住民参加
    奥祐三郎, 巌城隆, スミヤ・ガンゾリク, 野中成晃, 井上貴史, 宮原俊之, 岡崎克則, 神谷正男
    獣医畜産新報 60 5 376 - 378 文永堂出版(株) 2007年 [査読無し][招待有り]
  • Kamiya, M, Lagapa, J. T. G, Nonaka, N, Ganzorig, S, Oku, Y, Kamiya, H
    Revue Scientifique et Technique de l'Office International des Epizooties 25 3 1055 - 1066 2006年12月 [査読有り][通常論文]
     
    The authors describe the current control strategies targeting definitive hosts of the most important zoonotic parasite in Japan, Echinococcus multilocularis. A dramatic increase in the prevalence of echinococcosis in foxes in Hokkaido (the second largest of Japan's islands), the invasion of wild foxes into urban areas, infection among pet and stray dogs, and the possibility of spreading the disease to the main island of Japan (Honshu) - all these pose significant threats to public health. Previous research findings and current strategies such as control measures against infections in wild foxes, suggest that it will be possible to eliminate echinococcosis in the future. The enforcement of a national reporting system for veterinarians, international collaboration, and the establishment of a Forum on Environment and Animals (FEA) give further reason to believe that success is possible. This is the first report of a multifaceted control strategy against echinococcosis in definitive hosts that includes collaborative efforts with local residents. This model might provide new ideas for Veterinary Services worldwide in their efforts to control other related zoonotic diseases.
  • Kanai Y, Nonaka N, Katakura K, Oku Y
    Parasitology international 55 4 313 - 315 2006年12月 [査読有り][通常論文]
  • Chang, S. L, Ooi, H. K, Nonaka, N, Kamiya, M, Oku, Y
    Journal of Helminthology 80 3 219 - 223 2006年09月 [査読有り][通常論文]
     
    The development of metacestodes and adult worms of Taenia asiatica in Mongolian gerbils (Meriones unguiculatus) were observed. Cysticerci were recovered from gerbils subcutaneously injected with hatched oncospheres. The recovery rate ranged from 0.1 to 3.2%. No cysticerci were recovered from the orally inoculated gerbils. The infectivity of the cysticerci recovered at 48 weeks post-infection was evaluated. Tapeworms were recovered on day 14 post-infection from the small intestine of 5 of 11 gerbils, with a recovery rate of 27% (6 worms recovered/22 worms inoculated). Three and four adult worms were recovered from two human volunteers who ingested five cysticerci after 4 months post-infection. In worms recovered from gerbils, segmentation and genital primordia in the posterior proglottids and hooklets in the residual rostellum were observed. The results indicate that gerbils can serve as an alternative intermediate host and that partial development of the adult worm stage occurs in gerbils.
  • N Nonaka, M Kamiya, Y Oku
    PARASITOLOGY INTERNATIONAL 55 sup1 S263 - S266 2006年 [査読有り][通常論文]
     
    Echinococcus multilocularis is distributed all over Hokkaido, the northern island of Japan. The prevalence in foxes has been around 40% in the last decade. Three trials of anthelmintic bait distribution have been conducted in Hokkaido to reduce the prevalence in foxes. In those trials, bait distribution was done along roads in the study area using cars and/or around fox breeding dens by hand. Changes in the prevalence in foxes were evaluated either by necropsy of captured foxes or by coproantigen and egg detection of faeces collected in field. All of the trials showed bait distribution was effective for the reduction of the prevalence in foxes; however, it was also suggested that a frequent and continuous baiting program is necessary for effective and stable control of the prevalence in foxes. As observed in some cities in Europe, urban foxes infected with the parasite were also recognized in Sapporo. A survey of pet dogs showed that 0.4% of surveyed dogs were determined infected. In addition, a dog which was transported from Hokkaido to the main island of Japan was found excreting E. multilocularis eggs. The results raised the public recognition of canine infections, which in turn lead to the modification of a Japanese law for infectious diseases and to the enforcement of a national reporting system of dogs infected with E. multilocularis by veterinarians. (C) 2005 Elsevier Ireland Ltd. All rights reserved.
  • Development of Taenia saginata asiatica metacestodes in SCID mice and its infectivity in human and alternative definitive hosts
    Chang, S. L, Nonaka, N, Kamiya, M, Kanai, Y, Ooi, H. K, Chung, W. C, Oku, Y
    Parasitology Research 96 2 95 - 101 2005年05月 [査読有り][通常論文]
  • Immune responses to oral infection with Echinococcus multilocularis protoscoleces in gerbils: modified lymphocyte responses due to the parasite antigen
    Kato, N, Nonaka, N, Oku, Y, Kamiya, K
    Parasitology Research 96 1 12 - 17 2005年04月 [査読有り][通常論文]
  • 飼い犬のエキノコックス感染とその診断
    野中成晃
    獣医畜産新報 58 4 341 - 342 2005年04月 [査読無し][通常論文]
  • Production and characterization of monoclonal antibodies against excretory/secretory products of adult Echinococcus granulosus, and their application to coproantigen detection
    Casaravilla, C, Malgor, R, Rossi, A, Sakai, H, Nonaka, N, Kamiya, M, Carmona, C
    Parasitology International 54 1 43 - 49 2005年03月 [査読有り][通常論文]
  • Modified cellular immune responses in dogs infected with Echinococcus multilocularis
    Kato, N, Nonaka, N, Oku, Y, Kamiya, M
    Parasitology Research 95 5 339 - 345 2005年03月 [査読有り][通常論文]
  • 犬のエキノコックス症
    野中成晃
    獣医寄生虫学会誌 3 1 17 - 19 2004年12月 [査読無し][通常論文]
  • Possibility of invasion of Echinococcus into Honshu with pet dogs from Hokkaido and overseas
    Doi, R, Matsuda, H, Uchida, A, Kanda, E, Kamiya, H, Konno, K, Tamashiro, H, Nonaka, N, Oku, Y, Kamiya, M
    Japanese Journal of Public Health 50 7 639 - 649 2003年07月 [査読有り][通常論文]
  • Potential remedy against Echinococcus multilocularis in wild red foxes using baits with anthelmintic distributed around fox breeding dens in Hokkaido, Japan
    Tsukada, H, Hamazaki, K, Ganzorig, S, Iwaki, T, Konno, K, Lagapa, J. T, Matsuo, K, Ono, A, Shimizu, M, Sakai, H, Morishima, Y, Nonaka, N, Oku, Y, Kamiya, M
    Parasitology 125 2 119 - 129 2002年08月 [査読有り][通常論文]
  • Gastric hyperplasia and parietal cell loss in Taenia taeniaeformis inoculated immunodeficient mice
    Lagapa, J. T, Konno, K, Oku, Y, Nonaka, N, Ito, M, Kamiya, M
    Parasitology International 51 1 81 - 89 2002年03月 [査読有り][通常論文]
  • Taenia taeniaeformis larval product induces gastric mucosal hyperplasia in SCID mice
    Oku, Y, Nonaka, N, Kamiya, M
    Japanese Journal of Veterinary Research 49 4 273 - 285 2002年02月 [査読有り][通常論文]
  • Prevalence and intensity of Echinococcus multilocularis in red foxes (Vulpes vulpes schrencki) and raccoon dogs (Nyctereutes procyonoides albus) in Otaru city, Hokkaido, Japan
    Yimam, A, Nonaka, N, Oku, Y, Kamiya, M
    Japanese Journal of Veterinary Research 49 4 287 - 296 2002年02月 [査読有り][通常論文]
  • Assessment of the epidemiological status of Echinococcus multilocularis in foxes in France using ELISA coprotests on fox faeces collected in the field
    Raoul, F, Deplazes, P, Nonaka, N, Piarroux, R, Vuitton, D. A, Giraudoux, P
    International Journal for Parasitology 31 14 1579 - 1588 2001年12月 [査読有り][通常論文]
  • Inhibitory effect of different UV lamps on the infectivity of taeniid eggs
    Lagapa, J. T, Konno, K, Oku, Y, Nonaka, N, Kamiya, M
    Parasitology Research 87 8 593 - 597 2001年08月 [査読有り][通常論文]
  • A survey of canine echinococcosis in Gobi Altai Province of Mongolia by coproantigen detection
    Zoljargal, P, Ganzorig, S, Nonaka, N, Oku, Y, Kamiya, M
    Japanese Journal of Veterinary Research 49 2 125 - 129 2001年08月 [査読有り][通常論文]
  • Seroprevalence of Taenia solium cysticercosis in pigs in Bahia state, northeastern Brazil
    Sakai, H, Barbosa Jr, H. V, Silva, E. M, Schlabitz, F. O, Noronha, R. P, Nonaka, N, Franke, C. R, Ueno, H
    American Journal of Tropical Medicine and Hygiene 64 5 268 - 269 2001年05月 [査読有り][通常論文]
  • First report of Trichinella nativa in red foxes (Vulpes vulpes schrencki) from Otaru city, Hokkaido, Japan
    Yimam, A. E, Oku, Y, Nonaka, N, Sakai, H, Morishima, Y, Matsuo, K, Rosa, G. L, Pozio, E, Yagi, K, Kamiya, M
    Parasitology International 50 2 121 - 127 2001年 [査読有り][通常論文]
  • Matsuo, K, Nonaka, N, Oku, Y, Kamiya, M
    Parasitology Research 86 6 521 - 523 2000年06月 [査読有り][通常論文]
     
    Mongolian gerbils (Meriones unguiculatus) were orally inoculated with 10,000 protoscoleces of Echinococcus multilocularis per head after being divided into five groups (A-E). Each group was dosed with prednisolone tertiary-butylacetate (PTBA) as follows: A, 0 mg B, 0.5 mg C, 2 mg D, 5 mg and E, 10 mg/head. All animals were injected subcutaneously with control solvent or PTBA every other day from 6 days pre- to 6 days post-infection. Autopsy was performed at 7 days post-infection. Doses of PTBA and the number of worms recovered showed a positive correlation (r = 0.929, P < 0.0001). In groups A, B and C, the predilection site of the worms in the small intestine could not be determined, while in group D the worms were found more in the anterior part. In group E, the predilection site was the anterior part, followed by the middle and the posterior parts of the small intestine (Fisher's test: P < 0.01). The number of worms recovered from the anterior and the middle part of the small intestine also correlated positively with PTBA dose (anterior part: r = 0.930, P < 0.0001, middle part: r = 0.917, P < 0.0001). All groups of the PTBA-treated animals showed significant loss of weight compared to the non-treated animals (P < 0.01).
  • Development and sexual maturation of Echinococcus vogeli in an alternative definitive host, Mongolian gerbil (Meriones unguiculatus)
    Matsuo, K, Shimizu, M, Nonaka, N, Oku, Y, Kamiya, M
    Acta Tropica 75 3 323 - 330 2000年05月 [査読有り][通常論文]
  • Preliminary study of the role of red foxes in Echinococcus multilocularis transmission in the urban area of Sapporo, Japan
    Tsukada, H, Morishima, Y, Nonaka, N, Oku, Y, Kamiya, M
    Parasitology 120 4 423 - 428 2000年04月 [査読有り][通常論文]
  • Dose dependency of prednisolone on the establishment of Echinococcus multilocularis infection in an alternative definitive host, Mongolian gerbil
    Matsuo, K, Tada, T, Nonaka, N, Oku, Y, Kamiya, M
    Japanese Journal of Veterinary Research 47 3-4 145 - 149 2000年02月 [査読有り][通常論文]
  • Echinococcosis multilocular and alveolar hydatid disease in Hokkaido Japan
    Furth, M, Kamiya, M, Sakai, H, Nonaka, N, Oku, Y, El-On, J
    Israel Journal of Veterinary Medicine 55 2 59 - 62 2000年 [査読有り][通常論文]
  • The spreading of sarcoptic mange among red foxes Vulpes vulpes and its impact on the local fox population in Shiretoko Peninsula, Hokkaido, Japan
    Tsukada, H, Okada, H, Yamanaka, M, Nonaka, N, Oku, Y
    Mammalian Science 39 2 247 - 256 1999年12月 [査読有り][通常論文]
  • Coproantigen survey of Echinococcus multilocularis prevalence of red foxes in Hokkaido, Japan
    Morishima, Y, Tsukada, H, Nonaka, N, Oku, Y, Kamiya, M
    Parasitology International 48 2 121 - 134 1999年08月 [査読有り][通常論文]
  • Hyperplasia of gastric mucosa in donor rats orally infected with Taenia taeniaeformis eggs and in recipient rats surgically implanted with the larvae in the abdominal cavity
    Konno, K, Oku, Y, Nonaka, N, Kamiya, M
    Parasitology Research 85 6 431 - 436 1999年06月 [査読有り][通常論文]
  • チンチラChinchilla brevicaudataに寄生していたTaenia crassiceps
    久木義一, 野中成晃, Ganzorig, S, 森嶋康之
    獣医畜産新報 52 6 449 - 452 1999年06月 [査読有り][通常論文]
  • Histopathology and physiopathology of gastric mucous hyperplasia in rats heavily infected with Taenia taeniaeformis
    Konno, K, Abella, J. A, Oku, Y, Nonaka, N, Kamiya, M
    Journal of Veterinary Medical Sciences 61 4 317 - 324 1999年04月 [査読有り][通常論文]
  • Evaluation of coproantigen diagnosis for natural Echinococcus multilocularis infection in red foxes
    Morishima, Y, Tsukada, H, Nonaka, N, Oku, Y, Kamiya, M
    Japanese Journal of Veterinary Research 46 4 185 - 189 1999年02月 [査読有り][通常論文]
  • Monitoring of Echinococcus multilocularis infection in red foxes in Shiretoko, Japan, by coproantigen detection
    Nonaka, N, Tsukada, H, Abe, N, Oku, Y, Kamiya, M
    Parasitology 117 2 193 - 200 1998年08月 [査読有り][通常論文]
  • Seroprevalence of Taenia solium cysticercosis in pigs in a rural community of Honduras
    Sakai, H, Sone, M, Castro, D. M, Nonaka, N, Quan, D, Canales, M, Ljungstrom, I, Sanchez, A. L
    Veterinary Parasitology 78 3 233 - 238 1998年08月 [査読有り][通常論文]
  • Time-course of antibody response in mice against oral infection with eggs of Echinococcus multilocularis
    Matsumoto, J, Yagi, K, Nonaka, N, Oku, Y, Kamiya, M
    Parasitology 116 5 463 - 469 1998年05月 [査読有り][通常論文]
  • Coproantigen detection in a survey of Echinococcus multilocularis infection among red foxes, Vulpes vulpes schrencki, in Hokkaido, Japan
    Sakai, H, Nonaka, N, Yagi, K, Oku, Y, Kamiya, M
    Journal of Veterinary Medical Science 60 5 639 - 641 1998年05月 [査読有り][通常論文]
  • Coproantigen detection in a routine fox survey of Echinococcus multilocularis infection in Hokkaido, Japan
    Sakai, H, Nonaka, N, Yagi, K, Oku, Y, Kamiya, M
    Parasitology International 47 1 47 - 51 1998年03月 [査読有り][通常論文]
  • Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay
    Malgor, R, Nonaka, N, Basmadjian, I, Sakai, H, Carambula, B, Oku, Y, Carmona, C, Kamiya, M
    International Journal for Parasitology 27 12 1605 - 1612 1997年12月 [査読有り][通常論文]
  • Role of host immune response in the occurrence of gastropathy in rats infected with larval Taenia taeniaeformis
    Abella, J. A, Oku, Y, Nonaka, N, Ito, M, Kamiya, M
    Journal of Veterinary Medical Science 59 11 1039 - 1043 1997年11月 [査読有り][通常論文]
  • Concomitant onset of hypergastrinemia, intragastric alkalinity and gastric hyperplasia, and numbers of antral G cells in Taenia taeniaeformis-infected rats
    Abella, J. A, Oku, Y, Konno, K, Altamirano, Z, Nonaka, N, Kamiya, M
    Parasitology International 46 2 97 - 104 1997年07月 [査読有り][通常論文]
  • Description of Moniliformis worms from gray red-backed vole, Clethrionomys rufocanus bedfordiae, in Hokkaido Japan
    Abe, N, Nonaka, N, Iwaki, T, Ganzorig, S, Alexander, J. A, Oku, Y, Kamiya, M
    Parasitology International 46 1 41 - 44 1997年03月 [査読有り][通常論文]
  • Time course of coproantigen excretion in Echinococcus multilocularis infections in foxes and an alternative definitive host, golden hamsters
    Nonaka, N, Iida, M, Yagi, K, Ito, T, Ooi, H. K, Oku, Y, Kamiya, M
    International Journal for Parasitology 26 11 1271 - 1278 1996年11月 [査読有り][通常論文]
  • Parenteral strobilar development of Echinococcus multilocularis in SCID mice
    Inohara, J, Playford, M, Nonaka, N, Ooi, H. K, Oku, Y, Kamiya, M
    Japanese Journal of Veterinary Research 44 1 1 - 12 1996年05月 [査読有り][通常論文]
  • Acquired resistance against adult Echinococcus multilocularis infection observed in golden hamsters
    Inohara, J, Nonaka, N, Ooi, H. K, Oku, Y, Kamiya, M
    Japanese Journal of Parasitology 45 1 1 - 5 1996年02月 [査読有り][通常論文]
  • In vitro maintenance of Eperythrozoon suis
    Nonaka, N, Thacker, B. J, Schillhorn van Veen, T. W, Bull, R. W
    Veterinary Parasitology 61 3-4 181 - 199 1996年02月 [査読有り][通常論文]
  • Foraging behavior of red foxes Vulpes vulpes schrencki utilizing human food in the Shiretoko National Park, Hokkaido
    Tsukada, H, Nonaka, N
    Mammal Study 21 2 137 - 151 1996年 [査読有り][通常論文]
  • Antibody production in Syphacia obvelata infected mice
    Sato, Y, Ooi, H. K, Nonaka, N, Oku, Y, Kamiya, M
    Journal of Parasitology 81 4 559 - 562 1995年08月 [査読有り][通常論文]
  • Developmental and morphological characteristics of Taenia taeniaeformis (Batsch, 1786) in Clethrionomys rufocanus bedfordiae and Rattus norvegicus from different geographical locations
    Iwaki, T, Nonaka, N, Okamoto, M, Oku, Y, Kamiya, M
    Journal of Parasitology 80 3 461 - 467 1994年06月 [査読有り][通常論文]
  • Infectivities of four isolates of Taenia taeniaeformis to various rodents
    Nonaka, N, Iwaki, T, Okamoto, M, Ooi, H. K, Oku, Y, Ohbayashi, M, Kamiya, M
    Journal of Veterinary Medical Science 56 3 565 - 567 1994年06月 [査読有り][通常論文]
  • Survey on larval Echinococcus multilocularis and other hepatic helminths in rodents and insectivores in Hokkaido, Japan, from 1985 to1992
    Iwaki, T, Hatakeyama, S, Nonaka, N, Miyaji, S, Yokohata, Y, Okamoto, M, Ooi, H. K, Oku, Y, Kamiya, M
    Japanese Journal of Parasitology 42 6 502 - 506 1993年12月 [査読有り][通常論文]
  • A survey of helminth parasites in backyard flocks in Michigan by litter examination
    Nonaka, N, Donoghue, A. R, Manzoni, A. M, Schillhorn van Veen, T. W
    Avian Diseases 35 3 554 - 558 1991年07月 [査読有り][通常論文]

書籍

  • 日本獣医寄生虫学会 (担当:監修範囲:監修、第3章(総論)、第8章)
    緑書房 2020年09月 (ISBN: 9784895315692) 274p
  • 犬と猫 寄生虫症診断アトラス[内部寄生虫]
    Guaralupe Miro, Dwight D. Bow (範囲:監訳)
    ベーリンガーインゲルハイムアニマルヘルスジャパン 2020年04月
  • 板垣, 匡, 藤崎, 幸蔵, 筏井, 宏実, 井上, 昇, 奥, 祐三郎, 工藤, 上, 佐藤, 宏, 菅沼, 啓輔, 杉山, 広, 平, 健介, 田仲, 哲也, 野中, 成晃, 福本, 真一郎, 松林, 誠, 松本, 淳, 横山, 直明 (担当:分担執筆範囲:馬の条虫症、反芻動物の条虫症、家禽の条虫症)
    朝倉書店 2019年05月 (ISBN: 9784254460377) v, 359p
  • 動物病院スタッフのための犬と猫の感染症ガイド
    前田健, 佐藤宏 (担当:分担執筆範囲:Chapter3 犬の内部寄生虫感染症 3−7 エキノコックス症、3−9 鉤虫症、Chapter8 猫の内部寄生虫感染症 8−7 エキノコックス症、8−9 鉤虫症)
    緑書房 2019年04月
  • 臨床獣医師のための犬と猫の感染症診療
    小沼守、前田健、佐藤宏 (担当:分担執筆範囲:Chapter 3 犬の内部寄生虫感染症 3-7 エキノコックス症、3-9 鉤虫症、Chapter 8 猫の内部寄生虫感染症 8-7 エキノコックス症、8-9 鉤虫症)
    緑書房 2018年11月
  • 日本獣医寄生虫学会 (担当:監修範囲:監修、第3章(総論)、第7章)
    緑書房 2017年03月 (ISBN: 9784895312912) 229p
  • 木村, 哲, 喜田, 宏 (担当:分担執筆範囲:Vi. 原虫性人獣共通感染症 8.ジアルジア症、VII. 人獣共通寄生虫症 6.回虫症)
    医薬ジャーナル社 2016年02月 (ISBN: 9784753227778) 550p
  • 寄生虫病学共通テキスト編集委員会 (担当:編者(編著者)範囲:編集、第3章(総論)、第7章)
    緑書房 2014年06月 (ISBN: 9784895311601) 222p
  • 木村, 哲, 喜田, 宏 (担当:分担執筆範囲:Ⅵ原虫性人獣共通感染症 8. ジアルジア症、Ⅶ人獣共通寄生虫症 6. 回虫症)
    医薬ジャーナル社 2011年02月 (ISBN: 9784753224722) 551p
  • 前出, 吉光 (担当:分担執筆範囲:鉤虫症、回虫症、コクシジウム症、ジアルジア症、トリコモナス症、アメーバ大腸炎、鞭虫症)
    デーリィマン社 2007年03月 (ISBN: 9784938445669) 919p(図版共)
  • 前出吉光 (担当:分担執筆範囲:耳疥癬、鉤虫症、条虫症、回虫書、ジアルジア症、トキソプラズマ症、コクシジウム症、主要症状を基礎にした猫の臨床)
    デーリィマン社 2004年12月 (ISBN: 4938445182) 940
  • 共通感染症ハンドブック
    日本獣医師会 (担当:分担執筆範囲:鉤虫症)
    日本農村常法システム協会 2004年10月
  • 木村, 哲, 喜田, 宏 (担当:分担執筆範囲:Ⅵ原虫性人獣共通感染症 8.ジアルジア症、Ⅶ人獣共通寄生虫症 6.回虫症)
    医薬ジャーナル社 2004年08月 (ISBN: 475322094X) 447
  • Echinococcosis in central Asia: Problems and solutions
    Torgerson, P, Shaikenov, B (担当:分担執筆範囲:Effective countermeasures against alveolar echinococcosis in red fox population of Hokkaido, Japan)
    Publishing house "Dauir" 2004年
  • Technology innovation and its relations to humanities and social sciences
    Nakamura, M, Ki-Jun, L (担当:分担執筆範囲:Control and management of parasitic zonooses maintained in wildlife: A trial of Hokkaido University against echinococcosis)
    Hokkaido University Press 2003年
  • Helminths of wildlife
    Chowdhury, N, Aguirre, A. A (担当:分担執筆範囲:12 Japan)
    Science Publishers 2001年05月
  • 獣医寄生虫検査マニュアル
    今井壮一, 神谷正男, 平詔亨, 茅根士郎 (担当:分担執筆範囲:I.基礎編 2.寄生虫の検査・診断技術 2.6免疫学的検査法 (6)ウエスタンブロット)
    文永堂出版 1997年11月
  • Alveolar Echinococcosis: Strategy for eradication of alveolar echinococcosis of the liver
    Uchino, J, Sato, N (担当:分担執筆範囲:A diagnostic method for the definitive host of Echinococcus multilocularis by coproantigen detection)
    Fujishoin 1996年03月
  • A diagnostic method for the definitive host of Echinococcus multilocularis by coproantigen detection
    Alveolar Echinococcosis ; Strategy for Eradication of Alveolar Echinococcosis of the Liver (Fujishoin) 1996年
  • Biodiversity: Its Complexity and Role
    Yasuno, M, Watanabe, M (担当:分担執筆範囲:Diversity of taeniid parasites transmitted thorugh predator-prey interaction)
    Global Environmental Forum 1994年

作品等

  • 中南米におけるテニア科条虫の疫学調査
    1995年 -2000年
  • Epidemiology of taeniid cestodes in Latin America
    1995年 -2000年
  • エキノコックス終宿主診断法の開発
    1997年 -1999年
  • Development of a diagnostic method for the definitive host of Echinococcus
    1997年 -1999年
  • ウルグアイ・アルゼンチンにおける単包条虫の疫学調査
    1999年
  • ホンジュラス・オランチョ県におけるブタ有鉤嚢虫の疫学調査
    1999年
  • Epidemiological Study of Echinococcus granulosus in Uruguay and Argentina
    1999年
  • A survey of Taenia solium cysticercosis in pigs in Orancho, Honduras
    1999年
  • エキノコックス症感染源対策
    1997年
  • ブラジル・サルバドールにおけるブタ有鉤(]J2276[)虫感染の疫学調査
    1997年
  • Control for the definitive hosts of Echinococcus
    1997年
  • A survey of Taenia solium cysticercosis in pigs in Salvador, Brazil
    1997年
  • ウルグアイにおける単包条虫終宿主に対する診断法の開発と疫学調査
    1995年
  • Development of diagnostic method for the definitive host of Echinococcus granulosus and its application to epidemiological study
    1995年
  • アラスカにおける多包条虫疫学調査
    1989年
  • Epidemiological Study of Echinococcus multilocularis in Alaska
    1989年

その他活動・業績

  • 八木欣平, 奥祐三郎, 浦口宏二, 孝口裕一, 山野公明, 入江隆夫, 野中成晃, 福本真一郎, 森嶋康之, 小林文夫, 神谷正男, 神谷正男, 吉川泰弘 日本寄生虫学会大会プログラム・抄録集 85th 81 2016年02月 [査読無し][通常論文]
  • 倉田愛子, 関口敏, 桐野有美, 入江隆夫, 野中成晃, 堀井洋一郎, 下島昌幸, 黒田嘉紀, 乗峰潤三 獣医疫学雑誌 19 (1) 20 -21 2015年07月20日 [査読無し][通常論文]
  • 八木欣平, 神谷正男, 小林文夫, 斎藤通彦, 野中成晃, 関口敏, 奥祐三郎, 梅田滋, 加地祥文, 岡崎克則, 黄鴻堅, 高橋徹, 高橋俊幸, 浦口宏二, 山野公明, 孝口裕一, 入江隆夫 動物由来感染症に対するリスク管理手法に関する研究 平成26年度 総括・分担研究報告書 132 -145 2015年 [査読無し][通常論文]
  • 肉用鶏農場における鶏および鶏舎環境から分離されたカンピロバクターおよびサルモネラのMLST型別法による分子疫学的解析
    山崎 渉, 上村 涼子, 関口 敏, Dong Jianbao, 渡邉 真治, 桐野 有美, 目堅 博久, 谷口 喬子, 野中 成晃, 乗峰 潤三, 末吉 益雄, 後藤 義孝, 堀井 洋一郎, 黒木 真理子, 吉野 修司, 三澤 尚明 日本カンピロバクター研究会総会抄録集 7回 11 -11 2014年12月 [査読無し][通常論文]
  • 肉用鶏農場における鶏および鶏舎環境から分離されたカンピロバクターおよびサルモネラのMLST型別法による分子疫学的解析
    山崎 渉, 上村 涼子, 関口 敏, 董 建宝, 渡邉 真治, 桐野 有美, 目堅 博久, 谷口 喬子, 野中 成晃, 乗峰 潤三, 末吉 益雄, 後藤 義孝, 堀井 洋一郎, 三澤 尚明 日本獣医学会学術集会講演要旨集 157回 444 -444 2014年08月 [査読無し][通常論文]
  • 入江 隆夫, 太田 藍乃, 坂口 浩平, 日高 華奈子, 桐野 有美, 野中 成晃, 堀井 洋一郎 牛臨床寄生虫研究会誌 4 (1) 16 -16 2014年02月 [査読無し][通常論文]
  • 八木欣平, 神谷正男, ガンゾリグ スミヤ, 小林文夫, 斎藤通彦, 野中成晃, 関口敏, 奥祐三郎, 党志勝, 梅田滋, 加地祥文, 岡崎克則, 黄鴻堅, 高橋徹, 高橋俊幸, 浦口宏二, 山野公明, 孝口裕一, 入江隆夫 動物由来感染症に対するリスク管理手法に関する研究 平成25年度 総括・分担研究報告書 110 -117 2014年 [査読無し][通常論文]
  • 馬伝染性貧血ウイルス感染のnested PCRおよびreal-time PCRを用いた検出法の開発(Development of nested PCR and real-time PCR assays to detect equine infectious anemia virus infection)
    董 建宝, 朱 偉, 芳賀 猛, Cook Frank, 後藤 義孝, 桐野 有美, 野中 成晃, 堀井 洋一郎 日本獣医学会学術集会講演要旨集 156回 272 -272 2013年08月 [査読無し][通常論文]
  • 近畿・中国・四国地方におけるイノシシ猟犬の肺吸虫感染状況調査
    入江 隆夫, 山口 洋平, Doanh P.N, 波部 重久, 野中 成晃, 堀井 洋一郎 獣医寄生虫学会誌 10 (1-2) 19 -19 2012年03月 [査読無し][通常論文]
  • 糞便内肺吸虫卵検査におけるホルマリンエーテル法(MGL法)の再検討と新しい検査法、エーテルトラップ法の提案
    山口 洋平, 入江 隆夫, 数面 麻子, 野中 成晃, 堀井 洋一郎 獣医寄生虫学会誌 10 (1-2) 20 -20 2012年03月 [査読無し][通常論文]
  • 入江隆夫, 坂口浩平, 日高華奈子, 野中成晃, 野中成晃, 堀井洋一郎, 堀井洋一郎 日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州) 2012 49 2012年 [査読無し][通常論文]
  • 山口洋平, 入江隆夫, 数面麻子, 野中成晃, 堀井洋一郎 日本獣医学会学術集会講演要旨集 152nd 199 2011年08月31日 [査読無し][通常論文]
  • 入江隆夫, 山口洋平, NGOC Doanh Pham, 野中成晃, 堀井洋一郎 日本獣医学会学術集会講演要旨集 152nd 198 2011年08月31日 [査読無し][通常論文]
  • 中国・四国地方におけるイノシシ猟犬の肺吸虫血清疫学調査
    山口 洋平, 入江 隆夫, 三堂 祥吾, 南野 知也, 郭 志宏, 小林 郁雄, 野中 成晃, 堀井 洋一郎 獣医寄生虫学会誌 9 (2) 129 -129 2011年03月 [査読無し][通常論文]
  • 中国・四国地方におけるイノシシ猟犬の肺吸虫血清疫学調査
    山口 洋平, 入江 隆夫, 三堂 祥吾, 南野 知也, 郭 志宏, 小林 郁雄, 野中 成晃, 堀井 洋一郎 日本獣医学会学術集会講演要旨集 150回 182 -182 2010年09月 [査読無し][通常論文]
  • 【人と動物の共通感染症最前線】北海道の野生動物に見られるトリヒナ症の流行状況とトリヒナの分類
    金井 祐太, 井上 貴史, 野中 成晃, 片倉 賢, 間野 勉, 奥 祐三郎 獣医畜産新報 62 (6) 465 -466 2009年06月 [査読無し][通常論文]
  • ザンビアの牛から検出された単包条虫のgenotypeについて
    中村 星太, 野中 成晃, I.G.K.・フィリ, M, チェンベソフ, 井上 貴史, 松本 淳, 片倉 賢, 奥 祐三郎 獣医寄生虫学会誌 7 (2) 98 -98 2008年12月 [査読無し][通常論文]
  • 中村星太, 野中成晃, PHIRI Isaac G. K, CHEMBESOFU Mwelwa, 井上貴史, 松本淳, 片倉賢, 奥祐三郎 日本獣医学会学術集会講演要旨集 146th 174 -174 2008年09月05日 [査読無し][通常論文]
  • 野中成晃, 弘川治喜, 井上貴史, 中尾亮, ガンゾリク スミヤ, 小林文夫, 神谷正男, 神谷正男, 奥祐三郎 日本獣医学会学術集会講演要旨集 144th 57 2007年08月27日 [査読無し][通常論文]
  • 金井祐太, 野中成晃, 片倉賢, 奥祐三郎 日本寄生虫学会大会プログラム・抄録集 74th 98 2005年02月 [査読無し][通常論文]
  • 井上貴史, 金井祐太, 野中成晃, 片倉賢, 神谷正男, 奥祐三郎 日本寄生虫学会大会プログラム・抄録集 74th 75 2005年02月 [査読無し][通常論文]
  • 佐野隆史, 井上貴史, 福井大祐, 野中成晃, 片倉賢, 神谷正男, 奥祐三郎 日本寄生虫学会大会プログラム・抄録集 74th 75 2005年02月 [査読無し][通常論文]
  • Identification of taeniid cestode eggs - extraction of egg DNA and use of COI gene
    Japanese Journal of Veterinary Parasitology 3 (2) 31 2005年 [査読無し][通常論文]
  • A servey of gastrointestinal parasites in dogs and cats in Kanto district
    Japanese Journal of Veterinary Parasitology 3 (2) 47 2005年 [査読無し][通常論文]
  • Epizootiology of Echinococcus multilocularis in the northeastern region of Sapporo
    Japanese Journal of Veterinary Parasitology 3 (2) 30 2005年 [査読無し][通常論文]
  • 北海道に生息する移入動物アライグマ(Procyon lotor)の消化管内寄生蠕虫相の調査
    山田 大輔, 奥 祐三郎, 野中 成晃, 浅川 満彦, 池田 透, 淺野 玄, 赤松 里香, 的場 洋平, 神谷 正男 日本獣医学会学術集会講演要旨集 129回 60 -60 2000年03月 [査読無し][通常論文]

共同研究・競争的資金等の研究課題

  • エキノコックス終宿主における免疫反応の解明
    地域先端技術共同研究開発
    研究期間 : 1993年 -2010年
  • エキノコックス症の疫学調査とその対策
    地域先端技術共同研究開発
    研究期間 : 1993年 -2010年
  • Analysis of Intestinal Immunity in the Definitive Hosts of Echinococcus Multilocularis
    0035 (Japanese Only)
    研究期間 : 1993年 -2010年
  • Epidemiology and Control of Echinococcosis
    0035 (Japanese Only)
    研究期間 : 1993年 -2010年
  • エキノコックス終宿主診断
    地域先端技術共同研究開発
    研究期間 : 1993年 -2005年
  • Diagnosis of the definitive host of Echinococcus by coproantigen detection.
    0035 (Japanese Only)
    研究期間 : 1993年 -2005年

教育活動情報

主要な担当授業

  • 感染症学特別研究Ⅰ
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 国際感染症学院
  • 寄生虫学特論
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 感染症学特別演習
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 国際感染症学院
  • 寄生虫学特論
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 国際感染症学院
  • 感染症学特別研究ⅡA
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 国際感染症学院
  • 人獣共通感染症対策専門特論
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 国際感染症学院
  • 感染症学特別研究ⅡB
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 国際感染症学院
  • 原虫病学・寄生虫病学実習
    開講年度 : 2020年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 原虫、蠕虫、吸虫、条虫、線虫、節足動物、分類、形態、生活環、病態、疫学、診断、治療、種同定、宿主特異性、寄生適応、人獣共通感染症
  • 獣医科学特別研究
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 寄生虫・寄生虫病学
    開講年度 : 2020年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 蠕虫、吸虫、条虫、線虫、鉤頭虫、分類、発育、形態、生活環、病態、疫学、診断、治療、対策、宿主?寄生虫関係、人獣共通感染症
  • 獣医科学特論演習
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 原虫病学
    開講年度 : 2020年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 原虫、宿主動物、分類、形態、宿主特異性、生活環、疫学、病態、診断、治療、予防、節足動物、媒介、人獣共通感染症
  • 獣医科学基礎科目B 寄生虫学特論
    開講年度 : 2020年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 寄生虫・寄生虫病学
    開講年度 : 2020年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 原虫病学
    開講年度 : 2020年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 研究・臨床セミナー
    開講年度 : 2020年
    課程区分 : 学士課程
    開講学部 : 獣医学部

大学運営

委員歴

  • 2004年   日本寄生虫学会北日本支部会   評議員   日本寄生虫学会北日本支部会
  • 2004年   日本寄生虫学会   評議員   日本寄生虫学会


Copyright © MEDIA FUSION Co.,Ltd. All rights reserved.