松尾 和哉(マツオ カズヤ)
電子科学研究所 物質科学研究部門



  • 電子科学研究所 物質科学研究部門


  • 助教


  • 博士(工学)(京都大学)



  • 90764952

J-Global ID


  • モータータンパク質   分子機械   生物有機化学   ケミカルバイオロジー   


  • ナノテク・材料 / 生体化学
  • ナノテク・材料 / ケミカルバイオロジー
  • ナノテク・材料 / 生物分子化学


  • 2015年06月 - 現在 北海道大学 電子科学研究所 助教
  • 2014年06月 - 2015年05月 スクリプス研究所 博士研究員
  • 2014年04月 - 2014年05月 京都大学大学院 工学研究科 博士研究員


  • 2010年04月 - 2014年03月   京都大学大学院   工学研究科   合成・生物化学専攻
  • 2008年04月 - 2010年03月   名古屋市立大学大学院   薬学研究科
  • 2004年04月 - 2008年03月   名古屋市立大学   薬学部   薬学科


  • 日本薬学会   日本化学会   



  • Substrate selectivity and its mechanistic insight of the photo-responsive non-nucleoside triphosphate for kinesin and myosin
    Md. Jahirul Islam, Kazuya Matsuo, Halley M. Menezes, Masayuki Takahashi, Hidehiko Nakagawa, Akira Kakugo, Kazuki Sada, Nobuyuki Tamaoki
    17 53 - 65 2019年01月 [査読有り][通常論文]
  • Yoshimitsu Sagara, Marc Karman, Ester Verde-Sesto, Kazuya Matsuo, Yuna Kim, Nobuyuki Tamaoki, Christoph Weder
    Journal of the American Chemical Society 140 5 1584 - 1587 2018年02月07日 [査読有り][通常論文]
    The integration of mechanophores, motifs that transduce mechanical forces into chemical reactions, allows creating materials with stress-dependent properties. Typical mechanophores are activated by cleaving weak covalent bonds, but these reactions can also be triggered by other stimuli, and this renders the behavior unspecific. Here we show that this problem can be overcome by extending the molecular-shuttle function of rotaxanes to mechanical activation. A mechanically interlocked mechanophore composed of a fluorophore-carrying macrocycle and a dumbbell-shaped molecule containing a matching quencher was integrated into a polyurethane elastomer. Deformation of this polymer causes a fluorescence turn-on, due to the spatial separation of fluorophore and quencher. This process is specific, efficient, instantly reversible, and elicits an easily detectable optical signal that correlates with the applied force.
  • Kazuya Matsuo, Yuki Nishikawa, Marie Masuda, Itaru Hamachi
    Angewandte Chemie - International Edition 57 3 659 - 662 2018年01月15日 [査読有り][通常論文]
    The development of bioorthogonal approaches for labeling of endogenous proteins under the multimolecular crowding conditions of live cells is highly desirable for the analysis and engineering of proteins without using genetic manipulation. N-Sulfonyl pyridone (SP) is reported as a new reactive group for protein sulfonylation. The ligand-directed SP chemistry was able to modify not only purified proteins in vitro, but also endogenous ones on the surface of and inside live cells selectively and rapidly, which allowed to convert endogenous proteins to FRET-based biosensors in situ.
  • Ammathnadu S. Amrutha, K. R. Sunil Kumar, Kazuya Matsuo, Nobuyuki Tamaoki
    ORGANIC & BIOMOLECULAR CHEMISTRY 14 30 7202 - 7210 2016年 [査読有り][通常論文]
    Recently we demonstrated the photoregulation of the activity of kinesin-1 using an azobenzene-tethered peptide (azo-peptide: Azo-Ile-Pro-Lys-Ala-Ile-Gln-Ala-Ser-His-Gly-Arg-OH). To understand the mechanism behind this photoswitchable inhibition, here we studied the structure-property relationships of a range of azo-peptides through systematic variations in the structures of the peptide and azobenzene units. The vital peptide sequence for kinesin inhibition-mediated through electrostatic, hydrophobic and C-H center dot center dot center dot pi interactions-was the same as that for the self-inhibition of kinesin. We also identified substituents on the azobenzene capable of enhancing the photoswitchability of inhibition. As a result, we developed a new inhibitor featuring a relatively short peptide unit (-Arg-Ile-Pro-Lys-Ala-Ile-Arg-OH) and an azobenzene unit bearing a para-OMe group. In the trans form of its azobenzene unit, this finely tuned inhibitor stopped the kinesin-driven gliding motility of microtubules completely at a relatively low concentration, yet allowed gliding motility with a relatively high velocity in the cis form obtained after UV irradiation.
  • Yousuke Takaoka, Yoshiaki Fukuyama, Kazuya Matsuo, Itaru Hamachi
    CHEMISTRY LETTERS 42 11 1426 - 1428 2013年11月 [査読有り][通常論文]
    Herein, we report a novel signal-amplifiable self-assembling fluorescent nanoprobe for turn-on detection of specific enzyme activity. This probe employed a substrate of matrix metalloproteinase 2 (MMP2) together with a self-assembling unit, and showed a detection limit of less than 830 pM, which is 84-fold lower than that of our previous ligand-tethered fluorescent nanoprobes. Using this new probe, we succeeded in monitoring the specific activity of secreted MMP even in the conditioned media of tumor cells.
  • Kazuya Matsuo, Rui Kamada, Keigo Mizusawa, Hirohiko Imai, Yuki Takayama, Michiko Narazaki, Tetsuya Matsuda, Yousuke Takaoka, Itaru Hamachi
    CHEMISTRY-A EUROPEAN JOURNAL 19 38 12875 - 12883 2013年09月 [査読有り][通常論文]
    Specific turn-on detection of enzyme activities is of fundamental importance in drug discovery research, as well as medical diagnostics. Although magnetic resonance imaging (MRI) is one of the most powerful techniques for noninvasive visualization of enzyme activity, both in vivo and ex vivo, promising strategies for imaging specific enzymes with high contrast have been very limited to date. We report herein a novel signal-amplifiable self-assembling (FNMR)-F-19/MRI probe for turn-on detection and imaging of specific enzymatic activity. In NMR spectroscopy, these designed probes are silent when aggregated, but exhibit a disassembly driven turn-on signal change upon cleavage of the substrate part by the catalytic enzyme. Using these F-19 probes, nanomolar levels of two different target enzymes, nitroreductase (NTR) and matrix metalloproteinase (MMP), could be detected and visualized by (FNMR)-F-19 spectroscopy and MRI. Furthermore, we have succeeded in imaging the activity of endogenously secreted MMP in cultured media of tumor cells by (FMRI)-F-19, depending on the cell lines and the cellular conditions. These results clearly demonstrate that our turn-on F-19 probes may serve as a screening platform for the activity of MMPs.
  • Kazuyuki Aizawa, Hidehiko Nakagawa, Kazuya Matsuo, Kodai Kawai, Naoya Ieda, Takayoshi Suzuki, Naoki Miyata
    Bioorg. Med. Chem. Lett. 23 8 2340 - 2343 2013年04月 [査読有り][通常論文]
    Recent studies have shown that nitroxyl (HNO) ((HNO)-H-1/(NO)-N-3 ), which is the one-electron-reduced form of nitric oxide (NO), has unique biological activities, especially in the cardiovascular system, and HNO-releasing agents may have therapeutic potential. Since few HNO donors are available for use under physiological conditions, we synthesized and evaluated a series of Piloty's acid (PA) derivatives and evaluated their HNO-releasing activity under physiological conditions. N-Hydroxy-2-nitrobenzenesulfonamide (17) was the most efficient HNO donor among our synthesized PA derivatives, including the lead compound, 2-bromo-N-hydroxybenzenesulfonamide (2). The high HNO-releasing activity is suggested to be due to electronic and steric effects. Compound 17 may be a useful tool for biological experiments. (C) 2013 Elsevier Ltd. All rights reserved.
  • Kazuya Matsuo, Yoshiyuki Kioi, Ryosuke Yasui, Yousuke Takaoka, Takayuki Miki, Sho-hei Fujishima, Itaru Hamachi
    CHEMICAL SCIENCE 4 6 2573 - 2580 2013年 [査読有り][通常論文]
    Caged enzymes whose activities can be controlled by light represent a powerful tool for various biological analyses. However, limited methods are available for the construction of caged proteins and enzymes. We recently developed a novel protein labeling method termed ligand-directed acyl imidazole (LDAI) chemistry, which allows us to selectively modify natural dihydrofolate reductase and folate receptor in a test tube and in live cell contexts. In this work, we have examined in detail the reaction characteristics of the LDAI chemistry using carbonic anhydrase I (CAI) as a model enzyme. In addition to modifying Lys residues with a carbamate bond, the LDAI method modified Ser and Tyr residues with a carbonate bond. Owing to the relatively labile carbonate bond formed, the LDAI chemistry was demonstrated to be applicable for a rational one-step construction of caged enzymes. This method is simple and based on the transient tethering of an inhibitor with moderate activity that is directed to the active site on an enzyme surface. We successfully showed that the activity of the caged CAI was almost completely suppressed by LDAI-based labeling and fully recovered by photoirradiation in the crude conditions (such as cell lysates) as well as in test tube settings.
  • Kazuya Matsuo, Hidehiko Nakagawa, Yusuke Adachi, Eri Kameda, Kazuyuki Aizawa, Hiroki Tsumoto, Takayoshi Suzuki, Naoki Miyata
    CHEMICAL & PHARMACEUTICAL BULLETIN 60 8 1055 - 1062 2012年08月 [査読有り][通常論文]
    Nitroxyl (HNO), a one-electron-reduced form of nitric oxide, has various biological activities, including a cardioprotective effect. Here, we first synthesized another, more hydrophilic photocontrollable HNO donor (3), which can release HNO in a spatially and temporally controlled manner, and then examined the properties of our series of compounds as practical HNO donors in a cellular system under photocontrol. We selected compound 2 as the preferred donor, and used it to show that calcitonin gene-related peptide (CGRP) can be upregulated in A549 cells via photocontrolled HNO release. This result demonstrates the suitability of this photocontrollable HNO donor for biological investigations.
  • Yousuke Takaoka, Keishi Kiminami, Keigo Mizusawa, Kazuya Matsuo, Michiko Narazaki, Tetsuya Matsuda, Itaru Hamachi
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 133 30 11725 - 11731 2011年08月 [査読有り][通常論文]
    F-19 NMR/MRI probe is expected to be a powerful tool for selective sensing of biologically active agents owing to its high sensitivity and no background signals in live bodies. We have recently reported a unique supramolecular strategy for specific protein detection using a protein ligand-tethered self-assembling F-19 probe. This method is based on a recognition-driven disassembly of the nanoprobes, which induced a clear turn-on signal of F-19 NMR/MRI. In the present study, we conducted a systematic investigation of the relationship between structure and properties of the probe to elucidate the mechanism of this turn-on F-19 NMR sensing in detail. Newly synthesized F-19 probes showed three distinct behaviors in response to the target protein: off/on, always-on, and always-off modes. We clearly demonstrated that these differences in protein response could be explained by differences in the stability of the probe aggregates and that "moderate stability" of the aggregates produced an ideal turn-on response in protein detection. We also successfully controlled the aggregate stability by changing the hydrophobicity/hydrophilicity balance of the probes. The detailed understanding of the detection mechanism allowed us to rationally design a turn-on F-19 NMR probe with improved sensitivity, giving a higher image intensity for the target protein in F-19 MRI.
  • Kazuya Matsuo, Hidehiko Nakagawa, Yusuke Adachi, Eri Kameda, Hiroki Tsumoto, Takayoshi Suzuki, Naoki Miyata
    CHEMICAL COMMUNICATIONS 46 21 3788 - 3790 2010年 [査読有り][通常論文]
    A hydrophilic hetero-Diels-Alder cycloadduct was synthesized as a novel photocontrollable donor of reactive nitrogen species. Production of either nitric oxide (NO) or nitroxyl (HNO) was photoinduced from this compound, depending on the environmental polarity.
  • Yusuke Adachi, Hidehiko Nakagawa, Kazuya Matsuo, Takayoshi Suzuki, Naoki Miyata
    Chem. Commun. 41 5149 - 5151 2008年 [査読有り][通常論文]
    We synthesized hetero-Diels-Alder cycloadducts from acyl nitroso derivatives and 9,10-dimethylanthracene, to be photo-inducible HNO-releasing agents and found that introduction of conjugated nitroaromatic groups effectively enhanced the responsiveness of HNO release to UV-A irradiation; we confirmed photoinduced HNO formation by EPR and GCMS analysis.


  • Ligand-directed tosyl/acyl imidazole chemistry
    Kazuya Matsuo, Itaru Hamachi 
  • 生細胞でのタンパク質選択的なケミカルラベリングの新手法
    松尾 和哉, 浜地 格 
    生化学 2011年


  • モータータンパク質をオプトケミカルコントロールする手法の構築  [招待講演]
    松尾 和哉
    第6回アライアンス若手研究交流会 2018年11月 口頭発表(招待・特別)
  • 化学的手法による光応答性生体ナノマシンの開発  [通常講演]
    松尾 和哉, 玉置 信之
    第8回物質・デバイス領域共同研究拠点活動報告会及び29年度ダイナミック・アライアンス成果報告会 2018年06月 ポスター発表
  • アフィニティ駆動型化学修飾を利用した光制御型キネシンの開発  [通常講演]
    松尾 和哉, 玉置 信之
    日本薬学会 第138年会 2018年03月 口頭発表(一般)
  • ケミカルエンジニアリングによるモータータンパク質の機能改変  [通常講演]
    松尾 和哉, 玉置 信之
    第3回 北大部局間横断シンポジウム 2018年01月 ポスター発表
  • A Reversibly Photoswitchable Biomachine Constructed by Chemical Protein Engineering  [通常講演]
    Kazuya Matsuo, Nobuyuki Tamaoki
  • 化学修飾による光応答性モータータンパク質の構築  [通常講演]
    松尾 和哉, 玉置 信之
    第5 回アライアンス若手研究交流会 2017年08月 ポスター発表
  • 新規アゾベンゼン三リン酸によるキネシンの駆動とin vitro光制御  [通常講演]
    芦野 史弥, 松尾 和哉, 玉置 信之
    日本化学会第97春季年会(2017) 2017年03月 口頭発表(一般)
  • 可逆的に光制御できるキネシン阻害剤の開発とその応用  [通常講演]
    松尾 和哉, 玉置 信之
    日本化学会第97春季年会(2017) 2017年03月 口頭発表(一般)
  • Rational Design of Potent Photoreversible Kinesin-1 Inhibitors  [通常講演]
    Kazuya Matsuo, Nobuyuki Tamaoki
    The 17th RIES-HOKUDAI INTERNATIONAL SYMPOSIUM on Ju 2016年12月 ポスター発表
  • モータータンパク質キネシンの光制御型阻害剤における構造活性相関  [通常講演]
    松尾 和哉, 玉置 信之
    第4回アライアンス若手研究交流会 2016年11月 ポスター発表
  • Structure-Activity Relationship of Photochromic Inhibitors for Kinesin-1  [通常講演]
    Kazuya Matsuo, Ammathnadu S. Amrutha, K. R. Sunil Kumar, Nobuyuki Tamaoki
    HOKUDAI-NCTU Joint Symposium on Nano, Photo and Bio Sciences in 2016 2016年10月 ポスター発表
  • モータータンパク質キネシンを光制御する新規アゾベンゼン連結ペプチド  [通常講演]
    松尾 和哉, 玉置 信之
    日本化学会 第96春季年会 (2016) 2016年03月 口頭発表(一般)
  • リガンド指向型化学の新展開(1) : N-Sulfonyl Pyridone化学による細胞内タンパク質の特異的スルホニル化  [通常講演]
    松尾和哉, 増田真理恵, 浜地格
    日本化学会第94春季年会 2014年 口頭発表(一般)
  • 革新的ケミカルプローブによるバイオセンシング(1):自己会合性ナノプローブによる酵素活性Turn-Onイメージング  [通常講演]
    松尾和哉, 福山嘉晃, 鎌田瑠泉, 水澤圭吾, 今井宏彦, 高山裕生, 楢崎美智子, 松田哲也, 高岡洋輔, 浜地格
    日本化学会第93春季年会 2013年 口頭発表(一般)
  • 自己会合型プローブ(1):19F MRIプローブのタンパク質応答機構と高感度化  [通常講演]
    松尾和哉, 高岡洋輔, 木南啓司, 水澤圭吾, 楢崎美智子, 松田哲也, 浜地格
    日本化学会第92春季年会 2012年 口頭発表(一般)
  • 自己会合型分子プローブ(2) 19F NMR/MRIオフオンプローブの酵素系への応用  [通常講演]
    松尾和哉, 高岡洋輔, 湊大志郎, 築地真也, 浜地格
    日本化学会第91春季年会 2011年 口頭発表(一般)
  • 放出制御型HNOドナーの開発  [通常講演]
    松尾和哉, 中川秀彦, 安達祐介, 津元裕樹, 鈴木孝禎, 宮田直樹
    日本薬学会 第130年会 2010年 口頭発表(一般)
  • 水溶性光応答性HNO放出試薬の開発とその反応性  [通常講演]
    松尾和哉, 中川秀彦, 安達祐介, 津元裕樹, 鈴木孝禎, 宮田直樹
    第62回 日本酸化ストレス学会学術集会 2009年 口頭発表(一般)


  • 特願2008-284899:アシルニトロソ誘導体環化付加アントラセン誘導体および光作動型HNO供与体  
    宮田直樹, 中川秀彦, 鈴木孝禎, 松尾和哉


  • 2013年03月 日本化学会 第93回日本化学会春季年会 学生講演賞
    受賞者: 松尾 和哉


  • キネシンの細胞内物質輸送における分子機構の解明を目指した化学修飾法の開発
    研究期間 : 2016年04月 -2018年03月 
    代表者 : 松尾 和哉

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