研究者データベース

奥村 正裕(オクムラ マサヒロ)
獣医学研究院 獣医学部門 臨床獣医科学分野
教授

基本情報

所属

  • 獣医学研究院 獣医学部門 臨床獣医科学分野

職名

  • 教授

学位

  • 獣医学博士(北海道大学)

J-Global ID

研究キーワード

  • 関節疾患   獣医整形外科学   獣医学   獣医外科学   Establishment of precise diagnosis of joint disease in veterinary patients. Estimation of inflammation and inflammatory disorders by serological analysis.   

研究分野

  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学
  • ライフサイエンス / 獣医学

職歴

  • 2012年04月 - 現在 北海道大学 国際連携機構 アフリカルサカオフィス 所長
  • 2008年11月 - 現在 北海道大学 大学院獣医学研究科 教授
  • 2005年04月 - 2008年10月 北海道大学 大学院獣医学研究科 准教授
  • 2004年07月 - 2005年03月 北海道大学 大学院獣医学研究科 助教授
  • 2003年04月 - 2004年06月 北海道大学 大学院獣医学研究科 講師
  • 1993年10月 - 2003年03月 北海道大学 獣医学部 助手
  • 1990年07月 - 1992年07月 ザンビア大学 獣医学部基礎獣医学講座 講師

学歴

  • 1984年04月 - 1990年03月   北海道大学   獣医学部

所属学協会

  • 国際変形性関節症学会   日本軟骨代謝学会   欧州獣医整形外科外傷学会   日本リウマチ学会   日本獣医学会   日本ウマ科学会   獣医麻酔外科学会   Osteoarthritis Research Society International   Japanese Society of Cartilage Metabolism   European Society of Veterinary Orthopaedic and Traumatology   European Scoiety of Veterinary Orthopaedics and Traumatology   Japanese Society of Veterinary Anesthesiology and Surgery   Japanese Society of Equine Science   Society of Small Animal Veterinary Medicine   Society of Japanese Veterinary Medical Science   

研究活動情報

論文

  • Ekkapol Akaraphutiporn, Eugene C Bwalya, Sangho Kim, Takafumi Sunaga, Ryosuke Echigo, Masahiro Okumura
    The Journal of veterinary medical science 82 8 1209 - 1218 2020年08月28日 [査読有り]
     
    Pentosan polysulfate (PPS) is a semi-synthetic sulfated polysaccharide compound which has been shown the benefits on therapeutic treatment for osteoarthritis (OA) and has been proposed as a disease modifying osteoarthritis drugs (DMOADs). This study investigated the effects of PPS on cell proliferation, particularly in cell cycle modulation and phenotype promotion of canine articular chondrocytes (AC). Canine AC were treated with PPS (0-80 µg/ml) for 24, 48 and 72 hr. The effect of PPS on cell viability, cell proliferation and cell cycle distribution were analyzed by MTT assay, DNA quantification and flow cytometry. Chondrocyte phenotype was analyzed by quantitative real-time PCR (qPCR) and glycosaminoglycan (GAG) quantification. PPS significantly reduced AC proliferation through cell cycle modulation particularly by maintaining a significantly higher proportion of chondrocytes in the G1 phase and a significantly lower proportion in the S phase of the cell cycle in a concentration- and time-dependent manner. While the proportion of chondrocytes in G1 phase corresponded with the significant downregulation of cyclin-dependent kinase (CDK) 1 and 4. Furthermore, the study confirms that PPS promotes a chondrogenic phenotype of AC through significant upregulation of collagen type II (Col2A1) mRNA and GAG synthesis. The effect of PPS on the inhibition of chondrocyte proliferation while promoting a chondrocyte phenotype could be beneficial in the early stages of OA treatment, which transient increase in proliferative activity of chondrocytes with subsequent phenotypic shift and less productive in an essential component of extracellular matrix (ECM) is observed.
  • Ekkapol Akaraphutiporn, Takafumi Sunaga, Eugene C Bwalya, Ryosuke Echigo, Masahiro Okumura
    The Journal of veterinary medical science 82 6 793 - 803 2020年06月24日 [査読有り]
     
    This study investigated the effects of culture time on phenotype stability of canine articular chondrocytes (CACs) in non-passaged long-term monolayer culture. Third passage (P3) CACs isolated from four cartilage samples were seeded at three different initial seeding densities (0.2 × 104, 1.0 × 104 and 5.0 × 104 cells/cm2) and maintained in monolayer condition up to 8 weeks without undergoing subculture after confluence. The characteristic changes of chondrocytes during the culture period were evaluated based on the cell morphology, cell proliferation, glycosaminoglycans (GAGs) content, DNA quantification, mRNA expression and ultrastructure of chondrocytes. Chondrocytes maintained under post-confluence condition exhibited a capability to grow and proliferate up to 4 weeks. Alcian blue staining and Dimethylmethylene blue (DMMB) assay revealed that the extracellular matrix (ECM) synthesis was increased in a time-dependent manner from 2 to 8 weeks. The chondrocyte mRNA expression profile was dramatically affected by prolonged culture time, with a significant downregulation of collagen type I, whereas the expression of collagen type II, aggrecan, Sox9 and matrix metalloproteinase 13 (MMP-13) were significantly upregulated. In addition, transmission electron microscopy (TEM) result indicated dilation of rough endoplasmic reticulum (RER) in these long-term monolayer cultured chondrocytes. These findings demonstrate that the chondrocytes phenotype could be partially redifferentiated through the spontaneous redifferentiation process in long-term cultures using standard culture medium without the addition of chondrogenic supplements or tissue-culture scaffolds.
  • Sangho Kim, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    Journal of the American Veterinary Medical Association 255 3 330 - 335 2019年08月01日 [査読有り][通常論文]
     
    OBJECTIVE: To describe outcomes for dogs that underwent balloon dilation for palliative treatment of urethral obstruction caused by urothelial carcinoma. ANIMALS: 12 client-owned dogs. PROCEDURES: Medical records were searched to identify dogs with urothelial (bladder, urethra, or prostate) carcinoma that underwent balloon dilation for treatment of urethral obstruction between April 2010 and December 2015. Information regarding history, signalment, clinical signs, diagnostic imaging findings, balloon dilation technique, clinical outcomes, complications, and additional treatments was obtained by review of medical records. RESULTS: Improvement in clinical signs of urethral obstruction was observed after the initial dilation procedure for 9 of 12 dogs. Urethral obstruction was known to recur in 5 dogs 48 to 296 days after the initial procedure. Three of these dogs underwent a second dilation procedure, with clinical improvement in all 3 dogs for 41 to 70 days. One of 2 dogs that had a third procedure after the second reobstruction had clinical improvement in urinary tract signs until subsequent death from metastatic disease 22 days later. Complications included hematuria, urinary incontinence, and dysuria; these resolved within a few days after treatment. CONCLUSIONS AND CLINICAL RELEVANCE: Urethral balloon dilation was a minimally invasive procedure that provided relief of urethral obstruction from urothelial carcinoma in most dogs of the study population. Prospective studies are needed to identify optimal techniques for balloon dilation in dogs with neoplastic urethral obstructions and to identify patients that are likely to benefit most from the treatment.
  • Tatsuya Deguchi, Kenji Hosoya, Yusuke Murase, Sung Koangyong, Sangho Kim, Masahiro Okumura
    Veterinary and comparative oncology 17 2 119 - 129 2019年06月 [査読有り][通常論文]
     
    Cancer stem-like cells (CSCs) are self-renewing cells comprising a small subpopulation in tumours, and generate differentiated progeny through asymmetric division. It has been shown that CSCs are resistant to ionizing radiation, and this feature could be one of the mechanisms of tumour recurrence after radiation therapy. Much attention has been focused on to target CSCs; however, difficult of isolating CSCs and lack of knowledge on their radiosensitivity have limited this kind of research in veterinary medicine. In the present study, sphere-forming cells (SC), cultured using sphere formation method, were isolated from four type of canine tumour cell lines and evaluated if they have CSCs-like properties by expression of CSCs markers (real-time polymerase chain reaction) and capacity of tumorigenesis (xenograft transplantation in nude mice), and were assessed radiosensitivity (clonogenic survival assay) and DNA repair kinetics (immunofluorescence staining for p53-binding protein 1) after X-ray irradiation in comparison with the corresponding normal adherent culture cells (AC). All SCs were isolated using sphere formation and showed high gene expression of CD133 and tumorigenic ability as compared with AC. All SCs were significantly resistant against X-ray irradiation as compared with AC. In addition, the amount of DNA double-strand breaks after X-ray irradiation were significantly lower in SC compared with the corresponding AC. These results indicate that SC isolated through sphere formation possess CSCs-like characteristics and CSCs are important factor that affect radiosensitivity in canine tumours. In addition, radioresistance of CSCs may depend on reaction of DNA double-strand break after X-ray exposure.
  • Sangho Kim, Kenji Hosoya, Ayumi Kobayashi, Masahiro Okumura
    Veterinary and comparative oncology 17 1 61 - 68 2019年03月 [査読有り][通常論文]
     
    Peripheral blood stem cell (PBSC) transplantation following consolidation therapy is a feasible treatment option for canine haematological malignancies. In veterinary medicine, haematopoietic stem cells are generally mobilized into peripheral circulation using a granulocyte colony-stimulating factor (G-CSF). This pilot study aimed to evaluate the haematopoietic stem cell mobilization effect of three different regimens for PBSC apheresis with Spectra Optia continuous mononuclear cell (CMNC) protocol in healthy dogs. Stem cell mobilization was performed using high-dose plerixafor (CXCR-4 inhibitor) alone, a G-CSF alone, or a combination of the low-dose plerixafor and G-CSF. Three dogs were assigned to each mobilization protocol. Regardless of the mobilization protocol, the total blood volume processed was uniformly set as 270 mL/kg and many PBSCs, defined as CD34+/CD45dim cells, within the apheresis product were compared. Changes in complete blood count, PBSC counts, and blood chemistry analysis were monitored before, during, and after apheresis. All dogs tolerated the apheresis procedure using the Spectra Optia system with minimal adverse effects. The mean PBSC counts of the apheresis products for plerixafor, G-CSF, and the combination groups were 1.3 ± 0.24, 4.2 ± 0.47, and 6.4 ± 0.9 × 106 cells/kg, respectively. The apheresis procedure using Spectra Optia CMNC protocol in dogs is safe and feasible. Furthermore, PBSC mobilization with a combination of G-CSF and plerixafor appeared more effective than either compound alone in mobilizing PBSC to the peripheral blood in dogs.
  • H M Suranji Wijekoon, Sangho Kim, Eugene C Bwalya, Jing Fang, Keisuke Aoshima, Kenji Hosoya, Masahiro Okumura
    Research in veterinary science 122 179 - 185 2019年02月 [査読有り][通常論文]
     
    Pentosan polysulfate (PPS) is currently under investigation as a potential disease-modifying antiarthritic agent. In the present study the effects of PPS on arthritic profiles based on clinical score, ankle size, histological changes, and activity of inflammatory mediators using collagen-induced arthritic rat are reported. Model of arthritis was developed in Sprague Dawley rats by intradermal injection of bovine type II collagen emulsified with incomplete Freund's adjuvant. The rats were randomly divided into four groups: normal control, arthritic control, arthritic rats treated with PPS (at dose level 20 μg/g) and arthritic rats treated with meloxicam (2 μg/g). The treatment was continued daily until the day 30. Arthritic biomarkers (cartilage oligomeric matrix protein and tartrate-resistant acid phosphatase 5b) in synovial fluid, expression of inflammatory mediators (interleukin-1β, and tumor necrosis factor-α) and osteoclast marker genes (cathepsin K, tartrate-resistant acid phosphatase) in synovial membrane were measured. Daily administration of PPS to the arthritic rats significantly decreased the severity of arthritis by effectively suppressing the symptoms of arthritis and improving the functional recovery based on clinical score and histopathological evidence. Intriguingly, identical downregulation pattern of arthritis profiles, biological markers as well as relative mRNA levels of osteoclast markers and cytokines were monitored in arthritic rats treated with PPS. In conclusion, PPS exerted protective effects against collagen-induced arthritis in rats. The results suggest that PPS acts as an anti-inflammatory and anti-arthritic agent in decreasing the arthritic effects in collagen-induced arthritic rats.
  • Eugene C Bwalya, Hm Suranji Wijekoon, Jing Fang, Sangho Kim, Kenji Hosoya, Masahiro Okumura
    The Journal of veterinary medical science 80 11 1681 - 1687 2018年11月09日 [査読有り][通常論文]
     
    Although chondroinductive growth factors are considered necessary for chondrogenesis of bone marrow-derived mesenchymal stem cells (BMSC), independent and spontaneous chondrogenesis has been previously demonstrated in adult horses, bovine calves and adult human BMSC. Surprisingly, adult canine BMSC under similar culture conditions previously failed to demonstrate chondrogenesis. The present study evaluated independent chondrogenic potential of BMSC sourced from three young dogs in the absence of known chondroinductive factors. BMSC were culture expanded in 10% DMEM up to third passage (P3). At each passage, the phenotype of BMSC was evaluated by RT-PCR gel electrophoresis and qPCR. BMSC exhibited a chondrogenic phenotype in the absence of dexamethasone and TGF-β1 as verified by the expression of Sox-9, type II collagen and aggrecan. Sox-9 was significantly downregulated (P<0.05) from P1-P3 compared to P0 while type II and X collagen, and aggrecan were significantly downregulated at P3 compared to P0. There was a significant (P<0.01) negative correlation between passaging and Sox-9, type II collagen and aggrecan gene expression. These results indicate that independent chondrogenic potential and phenotype retention of BMSC decreases in a passage-dependent pattern. Therefore, caution should be exercised for future experiments evaluating the chondrogenic potential of BMSC after extensive expansion cultures in 10% DMEM.
  • Satoshi Takagi, Yumiko Kagawa, Kiwamu Hanazono, Shoko Murakami, Tatsuya Deguchi, Yusuke Izumi, Kenji Hosoya, Sangho Kim, Masahiro Okumura
    The Journal of veterinary medical science 80 9 1456 - 1458 2018年09月26日 [査読有り][通常論文]
     
    A 10-year-old cat presented for evaluation with a 1-month history of salivation and oral bleeding. A right mandibular mass was palpated and computed tomography examination revealed entire bone proliferation. Mandibular bone biopsy was performed, and histopathological diagnosis was vascular hamartoma. The cat suddenly died on day 140.
  • H M Suranji Wijekoon, Eugene C Bwalya, Jing Fang, Sangho Kim, Kenji Hosoya, Masahiro Okumura
    BMC veterinary research 14 1 152 - 152 2018年05月02日 [査読有り][通常論文]
     
    BACKGROUND: Sodium pentosan polysulfate (NaPPS) was testified as a chondroprotective drug in with a detailed rationale of the disease-modifying activity. This study was undertaken to determine whether anti-osteoarthritis drug, NaPPS inhibited osteoclasts (OC) differentiation and function. Canine bone marrow mononuclear cells (n = 6) were differentiated to OC by maintaining with receptor activator of nuclear factor kappa B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) for up to 7 days with the treatment of NaPPS at concentration of 0, 0.2, 1 and 5 μg/mL. Differentiation and function of OC were accessed using tartrate-resistant acid phosphate (TRAP) staining and bone resorption assay, while monitoring actin ring formation. Invasion and colocalization patterns of fluorescence-labeled NaPPS with transcribed gene in OC were monitored. Gene expression of OC for cathepsin K (CTK), matrix metallopeptidase-9 (MMP-9), nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), c-Fos, activator protein-1(AP-1) and carbonic anhydrase II was examined using real-time PCR. RESULTS: Significant inhibition of OC differentiation was evident at NaPPS concentration of 1 and 5 μg/mL (p < 0.05). In the presence of 0.2 to 5 μg/mL NaPPS, bone resorption was attenuated (p < 0.05), while 1 and 5 μg/mL NaPPS achieved significant reduction of actin ring formation. Intriguingly, fluorescence-labeled NaPPS invaded in to cytoplasm and nucleus while colocalizing with actively transcribed gene. Gene expression of CTK, MMP-9 and NFATc1 were significantly inhibited at 1 and 5 μg/mL (p < 0.05) of NaPPS whereas inhibition of c-Fos and AP-1 was identified only at concentration of 5 μg/mL (p < 0.05). CONCLUSIONS: Taken together, all the results suggest that NaPPS is a novel inhibitor of RANKL and M-CSF-induced CTK, MMP-9, NFATc1, c-Fos, AP-1 upregulation, OC differentiation and bone resorption which might be a beneficial for treatment of inflammatory joint diseases and other bone diseases associated with excessive bone resorption.
  • Suranji Wijekoon, Eugene C Bwalya, Jing Fang, Sangho Kim, Kenji Hosoya, Masahiro Okumura
    The Journal of veterinary medical science 79 12 2030 - 2035 2017年12月22日 [査読有り][通常論文]
     
    The aim of this study was to investigate osteoclastogenic properties of inflammatory cytokines at different time-points of osteoclastogenesis. Bone marrow-derived macrophages from five healthy dogs were stimulated with the macrophage colony-stimulating factor, receptor activator of nuclear factor-κB ligand and inflammatory cytokines such as interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-17. Osteoclasts (OC) formation and function were enhanced with TNF-α regardless of temporal differences. But in contrast, IL-1β suppressed the osteoclastogenesis at early phase of the process while upregulating at the late phase. Furthermore, differentiation of OC precursors into OC was suppressed at high concentrations of IL-17. Collectively, the results revealed that suppressing TNF-α would be a promising strategy to inhibit inflammation-associated bone destruction in dogs.
  • H M Suranji Wijekoon, Kazuhide Toyota, Sangho Kim, Jing Fang, Eugene C Bwalya, Kenji Hosoya, Masahiro Okumura
    Research in veterinary science 114 370 - 377 2017年10月 [査読有り][通常論文]
     
    The objective of this study was to assess the differentiation capability of synoviocytes derived from dogs with inflammatory joint conditions. Cranial cruciate ligament ruptured (CCLr) (n=12) and medial patella luxated (MPL) (n=10) knee joints of the dogs were used to collect the synovial membrane (SM). Synoviocytes were enzymatically released from the SM and analyzed by flow cytometry for specific cellular markers (CD44 and CD90) of mesenchymal stem cells (MSCs), while doing histopathology from another part of SM sections. Under specific culture conditions, synoviocytes were forced to differentiate into chondrogenesis, adipogenesis, osteogenesis and osteoclastogenesis to investigate the multipotency. Upon treatments phenotypes of cell cultures were analyzed by histopathology and by semi-quantitative reverse transcriptase polymerase chain reaction for the expression of each differentiation marker genes. Although flow cytometry showing similar MSCs populations in CCLr and MPL synovium, synovial cells derived from CCLr showed higher multipotency compared to MPL-derived samples. Further, synovial changes such as vascularity, mononuclear cell infiltration and cellular hypertrophy were more pronounced in CCLr-derived synovial tissue than in MPL. Taken together, these findings suggested that the differentiation capability of SM-derived multipotent stem cells varies with inflammatory severity occurring in different joint conditions.
  • Eugene C Bwalya, Sangho Kim, Jing Fang, H M Suranji Wijekoon, Kenji Hosoya, Masahiro Okumura
    The Journal of veterinary medical science 79 7 1182 - 1190 2017年07月07日 [査読有り][通常論文]
     
    Mesenchymal stem cells (MSC) are a potential alternative source of differentiated chondrocytes for cartilage tissue regeneration and repair of osteoarthritic (OA) joints. We investigated the effects of pentosan polysulfate (PPS) and polysulfated glycosaminoglycan (PSGAG) on chondrogenesis of canine bone marrow-derived mesenchymal stem cells (cBMSC) in alginate and micromass cultures (MMC). Chondrogenic differentiation medium (CDM) was supplemented with PPS or PSGAG at concentrations of 0 (positive control; PC), 1, 3 and 5 µg/ml. 10% DMEM was used as negative control. Chondrocyte phenotype was analyzed by quantitative real-time PCR (qPCR) for alginate cultures and Alcian blue staining for proteoglycan (PG) synthesis for MMC. In alginate culture, PPS and PSGAG showed no significant effect on type II collagen, aggrecan and HIF-2α mRNA expression. PPS had no significant effect on type I collagen whereas PSGAG significantly upregulated (P<0.05) it at all concentrations relative to other treatments. PPS demonstrated a dose-dependent inhibitory effect on type X collagen mRNA with significant inhibition observed at 5 µg/ml compared to the NC. PSGAG showed an inverse effect on type X collagen with 1 µg/ml significantly inhibiting its expression while increase in the concentration correspondingly increased type X collagen expression. In MMC, PPS significantly enhanced chondrogenesis and PG deposition whereas PSGAG inhibited chondrogenesis and promoted a fibrocartilage-like phenotype with reduced PG deposition. While PPS enhances chondrogenesis of cBMSC in MMC, the response of MSC to chondroinductive factors is culture system-dependent and varies significantly between alginate and MMC.
  • Yoshimi Iwaki, Satoshi Takagi, Keitaro Morishita, Kiwamu Hanazono, Kenji Hosoya, Masahiro Okumura
    JAPANESE JOURNAL OF VETERINARY RESEARCH 65 2 95 - 99 2017年05月 [査読有り][通常論文]
     
    A 10-year-old, spayed female dog with intermittent coughing and respiratory distress was presented at our hospital. Thoracic radiographs revealed a 71 x 43 mm intrathoracic solitary mass. After one week, coughing worsened and pleural effusion was found. The intrathoracic mass had the same intensity as the liver in contrast-enhanced computed tomography images. There were no signs of diaphragmatic hernia, and attachment of the mass to the diaphragm and the caudal vena cava was observed during surgery. Histopathologically, the mass was composed of normal hepatic parenchyma, and based on this finding and the gross findings, it was diagnosed as an ectopic liver. Owing to its location, the ectopic liver may have caused regional venous occlusion, which resulted in the clinical symptoms observed.
  • Bwalya EC, Kim S, Fang J, Wijekoon HMS, Hosoya K, Okumura M
    PloS one 12 5 e0177144  2017年 [査読有り][通常論文]
     
    Osteoarthritic (OA) chondrocytes are shown to express inducible nitric oxide synthase (iNOS) which produces high concentrations of nitric oxide (NO), particularly when stimulated with proinflammatory cytokines. NO is involved in OA cartilage degradation. On the other hand, c-Jun N-terminal Kinase (JNK) pathway mediates the activation and transcription of c-Jun, which is required for interleukin-1 (IL-1)-induction of matrix metalloproteinases-13 (MMP-13) in OA pathogenesis. Therefore, the selective inhibition of iNOS and c-Jun is a promising target for treatment and prevention of OA. The purpose of the study was to investigate the inhibitory effects of pentosan polysulfate (PPS) on IL-1β-induced iNOS, c-Jun and HIF-α isoforms upregulation in canine articular chondrocytes (CACs). Primary (P0) chondrocytes were isolated and cultured from femoral head cartilages of three (3) dogs. First passage (P1) chondrocytes were preincubated with 0, 1, 5, 15 and 40 μg/mL of PPS for 4 hr before treatment with 10 ng/mL rhIL-1β for a further 8 hr. In addition, we evaluated the effects of single and multiple cytokine with or without LPS on iNOS protein induction. PPS significantly inhibited (P < 0.05) IL-1β-induced iNOS, c-Jun and HIF-1α mRNA upregulation in a dose-dependent pattern. iNOS mRNA was significantly inhibited at 15 and 40 μg/mL whereas c-Jun and HIF-1α were significantly downregulated at 5, 15 and 40 μg/mL of PPS compared to chondrocytes treated with only rhIL-1β. Intriguingly, CACs were recalcitrant to single IL-1β, TNF-α or LPS-induction of iNOS protein including to a combination of IL-1β+TNF-α, IL-1β+LPS except to TNF-α+LPS and IL-1β+TNF-α+LPS suggestive of a protective mechanism from iNOS detrimental effects on perpetuating OA. IL-1β+TNF-α+LPS-induced iNOS protein expression was significantly abrogated by PPS. We demonstrate for the first time that PPS is a novel inhibitor of IL-1β-induced iNOS, c-Jun, and HIF-1α mRNA upregulation and iNOS protein induction which may be beneficial for prevention and treatment OA.
  • Sangho Kim, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION 51 6 407 - 412 2015年11月 [査読有り][通常論文]
     
    An 8 yr old, intact male Shiba Inu was presented with loose stool, polydipsia, hematuria, vomiting, and anorexia. On abdominal ultrasonography, numerous nodules were detected in the hepatic parenchyma distributed diffusely throughout all lobes. Excisional biopsy of one of the nodules was performed via exploratory laparotomy. A histopathological diagnosis of the lesion was carcinoid, and the tumor cells stained positive to chromogranin A and gastrin. The serum gastrin level of the dog was 45,613 pg/mL (reference range: 160-284). In addition to medical treatment with omeprazole(c) and famotidine(e), suppression of gastrin secretion was attempted with octreotide acetate. A test dose of octreotide acetate significantly decreased the serum gastrin level to approximately one third of the baseline in 2 hr and the effect lasted approximately for 6 hr. On day 21, treatment with sustained-release formulation of octreotide acetate(a) (5 mg intramuscular, q 4 wk) was initiated. The serum gastrin concentration gradually decreased over 32 days and then progressively increased in parallel with the progression of the hepatic nodules. The dog gradually developed recurrence of initial clinical signs, and was lost to follow-up on day 510.
  • Hiroki Yamazaki, Satoshi Takagi, Kenji Hosoya, Masahiro Okumura
    Veterinary journal (London, England : 1997) 205 3 375 - 80 2015年09月 [査読有り][通常論文]
     
    Histiocytic sarcoma (HS) in dogs exhibits aggressive biological behaviors and currently few effective treatments are available. Survivin could serve as a potential therapeutic target in several cancers. Sepantronium bromide (YM155) is a potential novel survivin-targeting agent and in this study the influence of survivin expression on clinical outcomes and the effects of YM155 on biological activities in HS cells were investigated. Specimens of HS dogs (n = 30) and four canine HS cell lines were used. The correlation between survivin expression and clinical outcome in the HS dogs was retrospectively assessed using quantitative PCR. Following YM155 treatment of cell lines, apoptosis, cell viability, and drug transporter activities were evaluated using annexin V staining, methylthiazole tetrazolium assays, and Hoechst-33342 staining, respectively. Elevated survivin expression in the HS dogs corresponded with reduced disease-free intervals and survival time, and increased chemoresistance, which led to poor clinical outcomes. Furthermore, YM155 treatment suppressed cell-growth and resistance to lomustine in HS cells by inhibiting the activity of ATP-binding cassette transporters. The evidence presented here supports favorable preclinical evaluation and indicates that survivin-targeted therapies might be effective against HS dogs.
  • Hiroki Yamazaki, Satoshi Takagi, Kenji Hosoya, Masahiro Okumura
    Research in veterinary science 99 137 - 44 2015年04月 [査読有り][通常論文]
     
    Histiocytic sarcoma (HS) in dogs exhibits aggressive clinical and biological behavior. Currently, no effective treatments are available for dogs with HS. Survivin, a member of a family of apoptosis protein inhibitors, could serve as a potential therapeutic target in several canine cancers. Sepantronium bromide (YM155) has recently been established as a novel survivin-targeting agent. The aim of this study was to use YM155 as a tool for evaluating survivin-targeted therapies against dogs with HS, and to investigate how YM155 treatment affects antitumor and chemotherapeutic efficacies in murine xenograft models using canine HS cells. The results showed that in HS cells with lomustine (CCNU) resistance, YM155 treatment suppressed both the cell-growth potential and cell resistance to CCNU, which essentially increases the chemotherapy efficacy in the murine models. The evidence presented here supports the favorable preclinical evaluation that survivin-targeted therapies might be effective against HS in dogs.
  • Hiroki Yamazaki, Tomomi Iwano, Saori Otsuka, Yumiko Kagawa, Yuki Hoshino, Kenji Hosoya, Masahiro Okumura, Satoshi Takagi
    Veterinary journal (London, England : 1997) 204 1 60 - 5 2015年04月 [査読有り][通常論文]
     
    Transitional cell carcinoma (TCC) in dogs is an aggressive malignant neoplasm, originating in the epithelium of the urinary bladder. The DEK nuclear protein is overexpressed in several types of human bladder cancer, where it is involved in chromatin reconstruction, gene transcription and apoptosis. Since DEK represents a potential therapeutic target for canine TCC, this study was designed to investigate DEK expression in canine TCC and to determine the effects of DEK mRNA silencing on TCC cells in vitro. The gene expression profiles of seven selected cancer-associated genes was assessed in four canine TCC cell lines and expression of DEK protein was evaluated in bladder tissue biopsies from healthy dogs and those affected with cystitis or TCC. After transfection of four canine TCC cell lines with DEK-specific or scrambled siRNA, annexin V staining was performed to evaluate apoptosis, and methylthiazole tetrazolium assays were performed to assess both cell viability and sensitivity to carboplatin. DEK mRNA expression was relatively high in canine TCC cells and expression of the DEK protein was significantly greater in TCC tumours compared with the other tissue samples. After transfection with DEK-specific siRNA, apoptosis, cell growth inhibition, and enhanced sensitivity to carboplatin were observed in all TCC cells assessed. These research findings suggest that DEK could be a potential therapeutic target for canine TCC.
  • Yusuke Izumi, Yuki Hoshino, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    The Journal of veterinary medical science 77 3 359 - 63 2015年03月 [査読有り][通常論文]
     
    The present study involved the isolation and characterization of canine tumor endothelial cells (TECs) from 2 malignancies. TECs were isolated using magnetic cell sorting following FITC labeling with UEA1 lectin, and they were characterized by measuring genetic and histopathological endothelial markers. Isolated TECs exhibited a cobblestone-like morphology and expressed both vascular endothelial growth factor receptor 2 (VEGFR2) and Von Willebrand factor (vWF). Further, both TECs and tumor cells derived from a seminoma exhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression. However, CXCR7 expression was not detected in TECs and tumor cells derived from a hepatocellular carcinoma. Understanding TEC specific traits may be important in the development of more efficacious anti-angiogenic therapies that do not induce adverse effects.
  • Sawang Kesdangsakonwut, Yuji Sunden, Keisuke Aoshima, Yoshimi Iwaki, Masahiro Okumura, Hirofumi Sawa, Takashi Umemura
    Neuropathology : official journal of the Japanese Society of Neuropathology 34 3 277 - 83 2014年06月 [査読有り][通常論文]
     
    Rabies is a fatal zoonotic disease for which no effective treatment measures are currently available. Rabies virus (RABV) has anti-apoptotic and anti-inflammatory properties that suppress nerve cell damage and inflammation in the CNS. These features imply that the elimination of RABV from the CNS by appropriate treatment could lead to complete recovery from rabies. Ten rabbits showing neuromuscular symptoms of rabies after subcutaneous (SC) immunization using commercially available vaccine containing inactivated whole RABV particles and subsequent fixed RABV (CVS strain) inoculation into hind limb muscles were allocated into three groups. Three rabbits received no further treatment (the SC group), three rabbits received three additional SC immunizations using the same vaccine, and four rabbits received three intrathecal (IT) immunizations, in which the vaccine was inoculated directly into the cerebrospinal fluid (the SC/IT group). An additional three naïve rabbits were inoculated intramuscularly with RABV and not vaccinated. The rabbits exhibited neuromuscular symptoms of rabies within 4-8 days post-inoculation (dpi) of RABV. All of the rabbits died within 8-12 dpi with the exception of one rabbit in the SC group and all four rabbits in SC/IT group, which recovered and started to respond to external stimuli at 11-18 dpi and survived until the end of the experimental period. RABV was eliminated from the CNS of the surviving rabbits. We report here a possible, although still incomplete, therapy for rabies using IT immunization. Our protocol may rescue the life of rabid patients and prompt the future development of novel therapies against rabies.
  • Namgil Oh, Sangho Kim, Kenji Hosoya, Masahiro Okumura
    The Journal of veterinary medical science 76 5 629 - 36 2014年05月 [査読有り][通常論文]
     
    The suppressive effects of nonsteroidal anti-inflammatory drugs (NSAIDs) on the bone healing process have remained controversial, since no clinical data have clearly shown the relationship between NSAIDs and bone healing. The aim of this study was to assess the compensatory response of canine bone marrow-derived mesenchymal stem cells (BMSCs) to several classes of NSAIDs, including carprofen, meloxicam, indomethacin and robenacoxib, on osteogenic differentiation. Each of the NSAIDs (10 µM) was administered during 20 days of the osteogenic process with human recombinant IL-1β (1 ng/ml) as an inflammatory stimulator. Gene expression of osteoblast differentiation markers (alkaline phosphatase and osteocalcin), receptors of PGE2 (EP2 and EP4) and enzymes for prostaglandin (PG) E2 synthesis (COX-1, COX-2, cPGES and mPGES-1) was measured by using quantitative reverse transcription-polymerase chain reaction. Protein production levels of alkaline phosphatase, osteocalcin and PGE2 were quantified using an alkaline phosphatase activity assay, osteocalcin immunoassay and PGE2 immunoassay, respectively. Histologic analysis was performed using alkaline phosphatase staining, von Kossa staining and alizarin red staining. Alkaline phosphatase and calcium deposition were suppressed by all NSAIDs. However, osteocalcin production showed no significant suppression by NSAIDs. Gene expression levels of PGE2-related receptors and enzymes were upregulated during continuous treatment with NSAIDs, while certain channels for PGE2 synthesis were utilized differently depending on the kind of NSAIDs. These data suggest that canine BMSCs have a compensatory mechanism to restore PGE2 synthesis, which would be an intrinsic regulator to maintain differentiation of osteoblasts under NSAID treatment.
  • H. Yamazaki, S. Takagi, N. Oh, Y. Hoshino, K. Hosoya, M. Okumura
    JOURNAL OF VETERINARY INTERNAL MEDICINE 28 1 204 - 210 2014年01月 [査読有り][通常論文]
     
    BackgroundDefinitive diagnosis of histiocytic sarcoma (HS) in dogs is relatively difficult by conventional histopathological examination because objective features of HS are not well defined. HypothesisQuantitative analysis of mRNA expression of selected cellular surface antigens (SAs) specific to HS in dogs can facilitate objective and rapid diagnosis. AnimalsDogs with HS (n=30) and dogs without HS (n=36), including those with other forms of lymphoma (n=4), inflammatory diseases (n=6), and other malignant neoplasias (n=26). MethodsRetrospective clinical observational study. Specimens were collected by excisional biopsy, needle core biopsy, or fine needle aspiration. To determine HS detection efficacy, mRNA expression levels of selected SAs specific to HS in dogs, including MHC class II, CD11b, CD11c, and CD86, were quantitatively analyzed using real-time quantitative polymerase chain reaction. ResultsEach SA mRNA expression level was significantly higher in HS dogs than in non-HS dogs (P=.0082). Cutoff values for discriminating between HS and non-HS dogs based on these expression levels were calculated on the basis of receiver-operating characteristic analysis. Accuracy of the cutoff values, including MHC class II, CD11b, CD11c, and CD86, was 87.9, 86.4, 86.4, and 84.8%, respectively. Conclusions and Clinical ImportanceOur results suggest that quantitative analysis of mRNA expression of the selected SAs could be an adjunctive diagnostic technique with high diagnostic accuracy for HS in dogs. Substantial investigation is required for exclusion of diseases with similar cell types of origin to lymphoma.
  • Namgil Oh, Takafumi Sunaga, Hiroki Yamazaki, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    The Japanese journal of veterinary research 61 3 97 - 107 2013年08月 [査読有り][通常論文]
     
    Cyclooxygenase (COX)-2 participates essentially in bone healing, demonstrated by COX-2 knockout mice that showed delayed fracture repair. Considerable controversy still exists on inhibitory effects of COX-2 inhibitors on bone healing in clinical cases. To assess stage-dependent effects of short-term treatment of COX-2 inhibitors on osteogenic differentiation, a canine POS osteosarcoma cell line which spontaneously differentiates into osteoblastic cell was exposed to COX-2 inhibitors such as carprofen and meloxicam for 72 hours during three different stages of osteoblast differentiation, including day 0 to 3 (pre-osteoblastic stage), day 4 to 7 (transitional stage) and day 8 to 11 (mature osteoblastic stage). As osteogenic markers, expression of alkaline phosphatase (ALP) was estimated by analysis of mRNA expression, enzymatic activity and ALP staining, and expression of osteocalcin was estimated by analysis of mRNA expression after the drug treatments. Calcified matrix formation was finally observed by von Kossa staining on day 14. Expressions of ALP showed no significant suppression by carprofen and meloxicam during all three stages. However, expressions of osteocalcin mRNA and non-calcified nodule formations were delayed by carprofen and meloxicam during transitional stage. Nevertheless, fully calcified nodule formation was observed in all experimental groups during post-medication period. These results indicate that short-term treatment of carprofen and meloxicam would reversibly suppress the differentiation of osteoblasts.
  • Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION 49 4 262 - 266 2013年07月 [査読有り][通常論文]
     
    A 5 yr old castrated male miniature dachshund presented with clinical signs attributable to carcinoma involving the bladder neck and prostate. On day 84 following diagnosis, the dog developed bilateral ureteral obstruction and ureteral stenting was attempted. The stents were inserted in a normograde fashion via percutaneous puncture of the dilated renal pelvises. Two wk later, the dog developed nodules at both sites of renocentesis. En block resection of the masses was performed, and histologic examination confirmed that the masses were urothelial carcinoma, likely caused by iatrogenic tumor seeding. Ureteral stenting is a useful technique to relieve malignant ureteral obstruction; however, risk of iatrogenic tumor seeding must be considered.
  • Akio Doi, Hiroki Takahashi, Bunei Syuto, Masaaki Katayama, Hiroyuki Nagashima, Masahiro Okumura
    Journal of Advanced Computational Intelligence and Intelligent Informatics 17 4 588 - 597 2013年 [査読有り][通常論文]
     
    We propose the use of a computer-aided design (CAD) system for treating bone fractures in small animals. During surgical planning, the veterinarian sketches a simple plate by referring to computed tomography images. A CAD operator then uses polygonal approximation (triangulation) of the surface of the bone region to model the plate. After the approximated shape is imported into the CAD system as a triangular mesh, a detailed design of the plate is prepared by referring to the abovementioned sketch. The plate can be designed to match the bone surface since the plate surface follows the curvature of the surface of the exported triangular mesh. The bone shape and the plate are eventually converted into polygons, and a structural model identical to the fractured part of the bone can be reproduced using a 3D printer, which allows for alignment to be performed at full scale. In this study, we examine the applicability of the proposed system by designing the most appropriately shaped plates for bone fracture therapy for small dogs brought to a veterinary clinic for treatment.
  • Hiroki Yamazaki, Satoshi Takagi, Yuki Hoshino, Kenji Hosoya, Masahiro Okumura
    PloS one 8 11 e79810  2013年 [査読有り][通常論文]
     
    Canine histiocytic sarcoma (CHS) is an aggressive malignant neoplasm that originates from histiocytic lineage cells, including dendritic cells and macrophages, and is characterized by progressive local infiltration and a very high metastatic potential. Survivin is as an apoptotic inhibitory factor that has major functions in cell proliferation, including inhibition of apoptosis and regulation of cell division, and is expressed in most types of human and canine malignant neoplasms, including melanoma and osteosarcoma. To investigate whether survivin was expressed at high levels in CHS and whether its expression was correlated with the aggressive biological behavior of CHS, we assessed relation between survivin expression and CHS progression, as well as the effects of survivin inhibition on the biological activities of CHS cells. We comparatively analyzed the expression of 6 selected anti-apoptotic genes, including survivin, in specimens from 30 dogs with histiocytic sarcoma and performed annexin V staining to evaluate apoptosis, methylthiazole tetrazolium assays to assess cell viability and chemosensitivity, and latex bead assays to measure changes in phagocytic activities in 4 CHS cell lines and normal canine fibroblasts transfected with survivin siRNA. Survivin gene expression levels in 30 specimens were significantly higher than those of the other 6 genes. After transfection with survivin siRNA, apoptosis, cell growth inhibition, enhanced chemosensitivity, and weakened phagocytic activities were observed in all CHS cell lines. In contrast, normal canine fibroblasts were not significantly affected by survivin knockdown. These results suggested that survivin expression may mediate the aggressive biological activities of CHS and that survivin may be an effective therapeutic target for the treatment of CHS.
  • Yoichi Kumada, Kyoto Hamasaki, Aya Nakagawa, Eiju Sasaki, Tatsunori Shirai, Masahiro Okumura, Manami Inoue, Michimasa Kishimoto
    Journal of Immunological Methods 400-401 1 70 - 77 2013年 [査読有り][通常論文]
     
    In this study, we demonstrated the successful preparation of a Fab antibody-immobilized hydrophilic polystyrene (phi-PS) plate via one- and two-step solid-phase refolding methods. Both polystyrene-binding peptide (PS-tag)-fused Fd fragment of heavy chain (Fab H-PS) and full-length of light-chain (Fab L-PS) were individually produced in insoluble fractions of Escherichia coli cells, and they were highly purified in the presence of 8. M of urea. Antigen-binding activities of Fab antibody immobilized were correctly recovered by the one-step solid-phase refolding method that a mixture of Fab H-PS and Fab L-PS was immobilized in the presence of 0.5-2. M urea, followed by surface washing of the phi-PS plate with PBST. These results indicate that by genetic fusion of a PS-tag, a complex between Fab H and Fab L was efficiently immobilized on the surface of a phi-PS plate even in the presence of a low concentration of urea, and was then correctly refolded to retain its high antigen-binding activity via removal of the urea.A two-step solid-phase refolding method whereby Fab H-PS and Fab L-PS were successively refolded on the surface of a phi-PS plate also resulted in Fab antibody formation on the plate. Furthermore, both the binding affinity and the specificity of the Fab antibody produced by the two-step method were highly maintained, according to the results of sandwich ELISA and competitive ELISA using Fab antibody-immobilized plate via two-step solid-phase refolding. Thus, the solid-phase refolding method demonstrated in this study should be quite useful for the preparation of a Fab antibody-immobilized PS surface with high efficiency from individually produced Fab H-PS and Fab L-PS. This method will be applicable to the preparation of a large Fab antibody library on the surface of a PS plate for use in antibody screening. © 2013 Elsevier B.V.
  • T Osaki, S Takagi, Y Hoshino, Y Aoki, Y Sunden, K Ochiai, M Okumura
    Australian veterinary journal 90 11 442 - 7 2012年11月 [査読有り][通常論文]
     
    A 5-year-old male cross-breed dog with chronic rhinosinusitis was presented. Computed tomography (CT) revealed opacities consistent with soft tissue or fluid in the left nasal cavity and frontal sinus. A diagnosis of chronic rhinosinusitis with myxomatous polyps was based on the histology of biopsy samples, so prednisolone treatment was administered for 1 month. Although the clinical signs slightly improved, no changes were observed on the CT images. At 8 months after the initial presentation, the dog was treated with antivascular photodynamic therapy (PDT) using benzoporphyrin derivative monoacid ring A and, 11 days later, CT revealed remarkably decreased soft tissue opacity; the facial swelling and sneezing also resolved. Three additional sessions of PDT were performed at 114, 210, and 303 days after the first PDT because of recurrence of clinical signs. The disease was well controlled for approximately 10 months by antivascular PDT.
  • Kazuya Edamura, Jonathan N King, Wolfgang Seewald, Nobuhiro Sakakibara, Masahiro Okumura
    The Journal of veterinary medical science 74 9 1121 - 31 2012年09月 [査読有り][通常論文]
     
    The efficacy and tolerability of robenacoxib for the treatment of osteoarthritis in dogs were evaluated in a prospective, multicenter, randomized, noninferiority design clinical trial. A total of 32 dogs presenting with osteoarthritis were allocated randomly to receive, orally once daily for 28 days, either 1-2 mg/kg robenacoxib (n=21) or 3.5-5 mg/kg carprofen (n=11). Dogs were assessed by clinicians and owners using numerical rating scale scores at baseline and days 14 and 28. The primary efficacy endpoint was the global functional disability score, which was the sum of clinician scores for standing posture, lameness at walk, lameness at trot, willingness to raise the contralateral limb and pain at palpation. There was a good to excellent level of efficacy in both treatment groups. Differences between days 14 and 28 compared to day 0 were significant for all 11 clinician and owner scores for robenacoxib, and for 6 of 11 scores for carprofen. The efficacy of robenacoxib was numerically superior to carprofen for all 13 endpoints, but differences were not statistically significant. For the global functional disability score, the estimated efficacy of robenacoxib was 1.244 (95% confidence interval 0.555-2.493) relative to carprofen. The tolerability of both treatments was good as assessed from adverse events, clinical signs, and hematology and serum biochemistry variables. In conclusion, once daily administration of robenacoxib tablets had noninferior efficacy and tolerability compared to carprofen for the treatment of the clinical signs of osteoarthritis in dogs.
  • Tadashi Sano, Jonathan N King, Wolfgang Seewald, Nobuhiro Sakakibara, Masahiro Okumura
    Veterinary journal (London, England : 1997) 193 2 397 - 403 2012年08月 [査読有り][通常論文]
     
    The objective of the study was to evaluate the efficacy and tolerability of robenacoxib, a selective cyclooxygenase-2 inhibitor, for the treatment of acute pain and inflammation associated with musculoskeletal disorders in cats. The study was a prospective, multi-centre, randomised, blinded, non-inferiority design clinical trial comparing robenacoxib to ketoprofen. A total of 68 cats presenting with pain and inflammation associated with acute musculoskeletal disorders were recruited and allocated randomly to receive, orally once daily for 5-6 days, either 1.0-2.4 mg/kg robenacoxib (n=47) or 1mg/kg ketoprofen (n=21). The primary efficacy endpoint was the total clinician score, which was the sum of clinician numerical rating scale scores for pain, inflammation and mobility. Assessments were made at baseline, on day 2, and day 4 or 5. For the total clinician score, non-inferior efficacy of robenacoxib was demonstrated with a relative efficacy of 1.151 (95% confidence interval 0.872-1.494). Non-inferior efficacy of robenacoxib was also demonstrated for the secondary endpoint of the total owner score. Robenacoxib was superior (P<0.05) to ketoprofen for the owner's assessment of activity and human/animal relationship. The tolerability of both treatments was good as assessed by monitoring adverse events, clinical signs and haematology and serum biochemistry variables.
  • Sunaga T, Oh N, Hosoya K, Takagi S, Okumura M
    The Journal of veterinary medical science / the Japanese Society of Veterinary Science 74 6 707 - 11 6 2012年06月 [査読有り][通常論文]
     
    Pentosan polysulfate sodium (PPS) has a heparin-like structure and is purificated from the plant of European beech wood. PPS has been used for the treatment of interstitial cystitis for human patients. Recent years, it was newly recognised that PPS reduce pain and inflammation of OA. The molecular biological mechanism of PPS to express its clinical effects is not fully understood. The purpose of the present study is to investigate a mechanism of action of PPS on inflammatory reaction of chondrocytes in vitro. It was evaluated that effects of PPS on interleukin (IL)-1β-induced phosphorylation of mitogen-actiated protein kinases (MAPKs), such as p38, extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), nuclear translocation of nuclear factor-kappa B (NF-κB), and matrix metalloproteinase (MMP)-3 production in cultured articular chondrocytes. As a result, in the presence of PPS existence, IL-1β-induced phosphorylation of p38 and ERK were certainly inhibited, while JNK phosphorylation was not affected. Nuclear translocation of NF-κB and MMP-3 production were suppressed by PPS pretreatment prior to IL-1β stimulation. In conclusion, it is strongly suggested that PPS treatment prevents inflammatory intracellular responses induced by IL-1 β through inhibition of phosphorylation of certain MAPKs, p38 and ERK and then nuclear translocation of NF-κB in cultured chondrocytes. These PPS properties may contribute to suppressive consequence of catabolic MMP-3 synthesis. These data might translate the clinical efficacy as PPS treatment could inhibit the cartilage catabolism and related clinical symptoms of OA in dogs.
  • Takafumi Sunaga, Namgil Oh, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    The Journal of veterinary medical science 74 6 745 - 50 2012年06月 [査読有り][通常論文]
     
    Canine osteoarthritis occurs frequently and causes secondary synovitis. Administration of nonsteroidal anti-inflammatory drugs (NSAIDs) is one of the major therapeutic options for pain management of joint diseases. Tepoxalin has an unique property as an NSAIDs that suppresses both cyclooxygenase and lipoxygenase. The purpose of this study was to evaluate antiproliferative effects of tepoxalin on cultured canine synovial cells. Cytotoxic effects of tepoxalin, carprofen, meloxicam and AA-861 on cultured canine synoviocytes were evaluated by MTT colorimetric assay. Apoptosis was detected by morphological observations with Giemsa or annexin V/Hoechst 33342 staining and by the inhibition of caspase-3 activity with N-Ac-Asp-Glu-Val-Asp-CHO (Ac-DEVD-CHO). Cytotoxic effects of tepoxalin were evident in comparison with the effects of carprofen or meloxicam. The same tendency of cytotoxicity was observed when 5-lipoxygenase was inhibited by AA-861. The morphological findings and contradictory effects of Ac-DEVD-CHO with regard to the cytotoxicity proved the proapoptotic effects of tepoxalin. In conclusion, tepoxalin might control osteoarthritic synovitis by inducing apoptosis in proliferating synoviocytes, while most NSAIDs that selectively inhibit cyclooxygenase-2 most likely would not suppress synovial proliferation.
  • Akio Doi, Hiroki Takahashi, Bunei Shuto, Masaaki Katayama, Hiroyuki Nagashima, Masahiro Okumura
    4TH INTERNATIONAL CONFERENCE ON AWARENESS SCIENCE AND TECHNOLOGY (ICAST 2012) 243 - 247 2012年 [査読有り][通常論文]
     
    We propose here the use of a computer-aided design (CAD) system in the treatment of fractures in small animals. During surgical planning, the veterinarian sketches a simple plate referring to computed tomography images. A CAD operator then models the plate by polygonal approximation (triangulation) of the surface of the bone region. After importing the approximated shape into the CAD system as a triangulation mesh, a detailed design of the plate is then prepared in the CAD system by referring to the abovementioned sketch. The plate can be designed to match the bone surface since the plate surface follows the curvature of the surface of the exported triangulation mesh. Furthermore, the bone shape and plate are eventually converted into polygon shapes, and a structural model identical to the fractured part of the bone can be reproduced using a 3D printer, allowing for alignment to be performed at full scale. In this study, we examined the applicability of the proposed system by designing the most appropriately shaped plate for bone fracture therapy in small dogs brought to a veterinary clinic for treatment.
  • Vivien Lettry, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    The Japanese journal of veterinary research 58 1 5 - 15 2010年05月 [査読有り][通常論文]
     
    Bone marrow derived mesenchymal stem cells (MSCs) can be used to repair articular cartilage defects, these cells should be properly stimulated so that they could differentiate morphologically and hold cellular synthetic features closer to maturely differentiated chondrocytes. It is well known that tissue specific environment plays an important role in cell fate determination. Once improved isolation, proliferation and differentiation protocols have been developed, the likelihood of spontaneous differentiation of MSCs into divergent lineages will be reduced, thus increasing their value for cartilage repair. The purpose of this study was to improve chondrogenic differentiation of equine MSCs using coculture with mature equine articular chondrocytes (ACs), along with the determination of the effect of adding transforming growth factor (TGF) beta1 in the pellet culture system. Following confirmation of multilineage (adipogenic, osteogenic and chondrogenic) differentiation, isolated MSCs, ACs and coculture of both cell types were transferred into pellet culture system in a DMEM-based medium supplemented with or without TGFbeta1. Chondrogenic differentiation was evaluated histologically and the relative mRNA expressions of collagen type 1 alpha1 (COL1A1), collagen type 2 alpha1(COL2A1), aggrecan (ACAN) and SRY-box 9 (SOX9) were estimated by quantitative RT-PCR. Cocultured cells showed diffuse distribution of extracellular matrix (ECM), whereas in chondrocyte pellets it was more localized to central regions. Expression of COL2A1, ACAN and SOX9 genes were higher in cocultured pellets when compared to MSCs and ACs-composed pellets. Addition of TGFbeta1 in chondrogenic differentiating medium did not consistently amplify expression of the above mentioned genes. Differentiation of equine MSCs was enhanced by coculturing in association with mature ACs, improving expression of cartilage-specific genes and producing a more homogeneous production of ECM within the newly formed cocultured cartilage. The use of the coculture system could possibly enhance the capacity of MSC-derived chondrocytes to build up stable articular cartilage-like constructs, which could play an important role in articular cartilage repair and regeneration.
  • Vivien Lettry, Yasuharu Sumie, Kenta Mitsuda, Masaaki Tagami, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    The Japanese journal of veterinary research 57 4 197 - 206 2010年02月 [査読有り][通常論文]
     
    The objective of this study was to investigate the changes in synovial fluid concentration of collagen type II cleavage site (C2C) and procollagen II C-propeptide (CPII), markers of joint cartilage degeneration and synthesis, respectively, in horses with intraarticular fracture or osteochondrosis dissecans (OCD), and to examine the relationship between arthroscopic findings and these biomarker levels. Synovial fluid was collected from 36 joints in 18 horses (6 fractures and 12 OCDs). Samples from contralateral normal joints, when available, served as controls (n = 12). Concentrations of C2C and CPII were measured using enzyme-linked immunosorbant assays. Moreover, the severity of the cartilage degradation was graded arthroscopically in 16 horses, and the correlation between the C2C and CPII levels and the arthroscopic scores were investigated. Compared to the control, the concentration of C2C was increased in OCD joints but not in fracture joints, whereas the concentration of CPII was increased in fracture joints but not in OCD joints. Within each disease group there was no correlation between biomarker levels and arthroscopic findings. Therefore, although C2C and CPII have diagnostic potential further knowledge is required to provide accurate analysis.
  • Vivien Lettry, Hirofumi Kawasaki, Kiyofumi Sugaya, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura
    The Japanese journal of veterinary research 57 4 207 - 12 2010年02月 [査読有り][通常論文]
     
    This study aimed to evaluate a system that identifies cartilage turn over and/or degradation through measurement of a new keratan sulfate (KS) epitope concentration in equine sera. Blood samples were collected from 30 horses, 1 (n = 15) and 2 year-olds (n = 15). Serum samples were analyzed for an epitope of keratan sulfate by 1/20/5D4 (KS5D4) and new epitopes of keratan sulfate using high sensitive keratan sulfate (HSKS), measured by two respective enzyme-linked immunosorbant assays (ELISAs). There was no correlation in serum concentration of KS evaluated using 5D4 and HSKS. Age had no significant effect on concentrations of KS measured with KS5D4 while 1 year-old horses showed significantly higher amounts than 2 year-olds with HSKS. Results suggest that HSKS could detect early signs of cartilage metabolic changes.
  • Tomohiro Osaki, Satoshi Takagi, Yuki Hoshino, Masahiro Okumura, Tsuyoshi Kadosawa, Toru Fujinaga
    JOURNAL OF VETERINARY MEDICAL SCIENCE 71 2 125 - 132 2009年02月 [査読有り][通常論文]
     
    Antivascular photodynamic therapy (PDT) Suppresses tumor growth and prolonged the survival in solid tumor-bearing mice. The purpose of this study was to assess the efficacy of antivascular PDT using BPD-MA for treatment of oral and nasal tumors in 14 dogs. At 15 min after initiating intravenous infusion of 0.5 mg/kg benzoporphyrin derivative monoacid ring A, tumors were irradiated with laser light at 690 nm emitted by a diode laser, The 1-year survival rate of 7 dogs with oral tumors was 71%. The 1-year Survival rate of 7 does with nasal tumors was 57%. Imaging of each tumor was performed by using angiographic computed tomography before and after each antivascular PDT. Contrast-enhanced turners were observed before antivascular PDT, but these tumors were not enhanced with contrast medium following antivascular PDT. Antivascular PDT is suggested to be a promising method for dogs with oral and nasal tumors that cannot be effectively treated with current antitumor therapies.
  • Yuki Hoshino, Satoshi Takagi, Tomohiro Osaki, Masahiro Okumura, Toru Fujinaga
    The Journal of veterinary medical science 70 6 581 - 8 2008年06月 [査読有り][通常論文]
     
    We attempted to accumulate the basic data for evaluation of activated lymphocyte therapy for small animal medicine. The peripheral blood mononuclear cells (PBMCs) collected from healthy dogs were activated using anti-CD3 antibody and human recombinant (hr) interleukin (IL)-2 and reactivated using hr interferon (IFN)-alpha and hr IL-2. The property of obtained cells was compared with PBMCs. The number of cells was shown to have increased approximately>50 -fold by cultivation. The proportion of CD8+ cells was significantly increased, the cytotoxicity of the cultured cells was revealed to have been reinforced. Additionally, CD56 mRNA levels tended to have increased. The cells obtained by this method were confirmed to be activated lymphocytes. Furthermore, we investigated the effects of sequential administration of the obtained cells to healthy dogs. By sequential administration of the activated lymphocytes, the cell proliferative activity, proportion of CD4+ cells and CD8+ cells, and serum IFN-gamma concentration were shown to have increased, and no severe adverse effects were observed. Consequently, activated lymphocytes could be induced using anti-CD3 antibody and IL-2 in healthy dogs, and sequential administration of activated lymphocytes reinforced the recipient's immunity.
  • Yuki Hoshino, Motoshi Tajima, Satoshi Takagi, Tomohiro Osaki, Masahiro Okumura, Toru Fujinaga
    JOURNAL OF VETERINARY MEDICAL SCIENCE 70 3 309 - 312 2008年03月 [査読有り][通常論文]
     
    Real-time PCR was optimized for the quantification of canine CD56 mRNA expression. This study was conducted to easily quantify canine CD56 expression and to identify its expression in normal tissues, peripheral blood mononuclear cells and activated lymphocytes in dogs. This assay revealed the highest level of CD56 mRNA expression in the normal canine brain, followed by the lung, kidney and liver. CD56 mRNA expression level in peripheral blood mononuclear cells was considerably lower; among activated lymphocytes in vitro, CD56 mRNA expression was increased.
  • Tomohiro Osaki, Satoshi Takagi, Yuki Hoshino, Masahiro Okumura, Toru Fujinaga
    Cancer letters 248 1 47 - 57 2007年04月08日 [査読有り][通常論文]
     
    Photodynamic therapy (PDT) using benzoporphyrin derivative monoacid ring A (BPD-MA) induces direct tumor cell damage and microvascular injury. We administered BPD-MA at 3h or 15min before laser irradiation to KLN205 and LM8 tumors in murine models. Tumor growth delay was induced more effectively by 15-min-interval PDT than by 3-h-interval PDT. Vascularity and blood perfusion was significantly decreased by 15-min-interval PDT. We observed death of all tumor cells, except peripheral cells, in the 3-h-interval PDT group, and death of cells around the damaged tumor vasculature in the 15-min-interval PDT group. Thus, 15-min-interval PDT enhanced the antitumor effect by damaging tumor vasculature.
  • S Takagi, Y Hoshino, T Osaki, M Okumura, T Fuginaga
    Experimental oncology 29 1 30 - 4 2007年03月 [査読有り][通常論文]
     
    AIM: It has been demonstrated that the endogenous matrix metalloproteinases (MMPs) inhibitor reversion inducing cysteine rich protein with Kazal motifs (RECK) is a reliable prognostic marker for detecting several types of tumors. However, the RECK expressions in most of the normal and neoplastic tissues were extremely low, and to measure its expression is quite complicated. The purpose of the present study is to establish an easy method to quantify murine RECK mRNA expression for use in future experimental studies. Subsequently, in order to verify the reliability of the established quantification technique, we examined the change in RECK expression and gelatinase secretion in tumor cells when stimulated by the extracellular matrix. METHODS: Several murine tumor cells were used in the present study. The real-time polymerase chain reaction (PCR) method and measurement conditions for murine RECK mRNA were studied using these tumor cells. Gelatinase activities were also examined by gelatin zymography. RESULTS: Murine RECK mRNA expression was accurately quantified using real-time PCR. Among the tumor cells used in the study, osteosarcoma cells showed significantly higher RECK mRNA expression than the others. The RECK expression in the osteosarcoma cells was down-regulated by contact with matrigel-coated culture flasks due to increased secretion of gelatinases. CONCLUSION: The real-time PCR method employed in our study is useful to quantify RECK expression.
  • Tomohiro Osaki, Satoshi Takagi, Yuki Hoshino, Masahiro Okumura, Toru Fujinaga
    Cancer letters 243 2 281 - 92 2006年11月18日 [査読有り][通常論文]
     
    The relative sensitivities of different tumor cells to photodynamic therapy (PDT) with benzoporphyrin derivative monoacid ring A (BPD-MA) were compared in the four tumor cells. A good correlation was observed between the cell survival at 0.1 microg/ml of BPD-MA and sensitizer uptake/10(6) cells (r = -0.99) or the plating efficiency of cells (r = 0.99). At 3 h after the irradiation, a significant difference was observed in the proportion of apoptotic cells among the four tumor cells (p = 0.024). In conclusion, cell responses to PDT depend on the several factors such as the cell line, photosensitizer dose, and fluence.
  • Darko Bosnakovski, Morimichi Mizuno, Gonhyung Kim, Satoshi Takagi, Masahiro Okumura, Toru Fujinaga
    Biotechnology and bioengineering 93 6 1152 - 63 2006年04月20日 [査読有り][通常論文]
     
    Bone marrow mesenchymal stem cells (MSCs) are candidate cells for cartilage tissue engineering. This is due to their ability to undergo chondrogenic differentiation after extensive expansion in vitro and stimulation with various biomaterials in three-dimensional (3-D) systems. Collagen type II is one of the major components of the hyaline cartilage and plays a key role in maintaining chondrocyte function. This study aimed at analyzing the MSC chondrogenic response during culture in different types of extracellular matrix (ECM) with a focus on the influence of collagen type II on MSC chondrogenesis. Bovine MSCs were cultured in monolayer as well as in alginate and collagen type I and II hydrogels, in both serum free medium and medium supplemented with transforming growth factor (TGF) beta1. Chondrogenic differentiation was detected after 3 days of culture in 3-D hydrogels, by examining the presence of glycosaminoglycan and newly synthesized collagen type II in the ECM. Differentiation was most prominent in cells cultured in collagen type II hydrogel, and it increased in a time-dependent manner. The expression levels of the of chondrocyte specific genes: sox9, collagen type II, aggrecan, and COMP were measured by quantitative "Real Time" RT-PCR, and genes distribution in the hydrogel beads were localized by in situ hybridization. All genes were upregulated by the presence of collagen, particularly type II, in the ECM. Additionally, the chondrogenic influence of TGF beta1 on MSCs cultured in collagen-incorporated ECM was analyzed. TGF beta1 and dexamethasone treatment in the presence of collagen type II provided more favorable conditions for expression of the chondrogenic phenotype. In this study, we demonstrated that collagen type II alone has the potential to induce and maintain MSC chondrogenesis, and prior interaction with TGF beta1 to enhance the differentiation.
  • T Osaki, S Hoshino, Y Hoshino, S Takagi, M Okumura, T Kadosawa, T Fujinaga
    Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 53 2 108 - 12 2006年03月 [査読有り][通常論文]
     
    The aim of this study was to examine the pharmacokinetics of clinically applied benzoporphyrin derivative monoacid ring-A (BPD-MA; Verteporfin), a second-generation photosensitizer, during a trial of photodynamic therapy (PDT) in nine dogs having naturally occurring neoplasms. After injecting BPD-MA at 0.5 mg/kg intravenously, its mean half-life (t1/2) was found to be 8.14 +/- 5.34 h, mean clearance (Cl) 35.13 +/- 9.62 ml/(h kg), the mean value of the volume of distribution (Vc) 0.08 +/- 0.01 l/kg and the mean steady state volume of distribution (Vss) 0.38 +/- 0.31 l/kg respectively. With the exception of a transitional increase in serum alkaline phosphatase activity, no other clinical abnormalities were observed. The t1/2 in dogs with naturally occurring tumours was longer than that in humans, but similar to that in rats. The values of Cl and Vss in dogs having naturally occurring neoplasms were lower than those in humans. It is suggested that the pharmacokinetics of BPD-MA in tumour-bearing dogs would be helpful in determining the protocol of a short drug-light interval PDT with BPD-MA that mainly targets the tumour vasculature.
  • D Bosnakovski, M Mizuno, G Kim, S Takagi, M Okumura, T Fujinaga
    JAPANESE JOURNAL OF VETERINARY RESEARCH 53 3-4 127 - 139 2006年02月 [査読有り][通常論文]
     
    Bovine bone marrow mesenchymal stem cells (MSCs) cultured in condensate culture, spontaneous and independent for any external biostimulants, undergo chondrogenic differentiation. In the present study, the bovine MSC chondrogenesis pathway was studied by analyzing stage-specific gene expression using quantitative "Real Time" reverse transcriptase polymerase chain reaction (qRT-PCR). Results showed that bovine MSCs underwent complete chondrogenesis; the initial stage was characterized by expression of sox9 messenger ribonucleic acid (mRNA), followed by high transcription of chondrocyte specific genes, collagen type II and IX, biglycan and cartilage ollgomeric matrix protein, and the final prehypertrophic and/or hypertrophic stage was distinguished by increased expression of collagen type X. From day 7 to day 14 of differentiation increased mRNA expression of the transforming growth factors beta 1 and beta 2, basic fibroblast growth factor (FGF 2), bone morphogenic protein 6 (BMP 6), insulin-like growth factors 1, parathyroid hormone related peptide and indian hedgehog (Ihh) were detected. These results suggest that these well know chondrogenic growth factors may play a role in bovine chondrogenesis in autocrine and/or paracrine manner. On day 21 of the culture, FGF 2, BMP 6 and Ihh were highly expressed, compared to cells cultured in monolayer manner, which suggests a possible function in maintaining the terminal stage of differentiation. This data extends our knowledge about the unusual species-specific bovine MSC chondrogenesis, allowing us to define the phenotype of the differentiated cells. Furthermore, this study contributes to our in understanding of known chondrogenic-growth factors in autocrine and/or paracrine manner playing a role in the spontaneous differentiation.
  • S Takagi, T Kadosawa, T Ishiguro, T Ohsaki, M Okumura, T Fujinaga
    The Journal of small animal practice 46 10 504 - 6 2005年10月 [査読有り][通常論文]
     
    A 15-year-old, male neutered cat was referred for investigation of dysuria. A retrograde urethrography was performed which showed two space-occupying masses within the lumen of the mid-to-proximal urethra. Exploratory coeliotomy revealed two urethral masses. Segmental urethrectomy was performed to resect the mass, and the lower urinary tract was reconstructed by vesico-urethral anastomosis. Histopathology showed the mass to be a transitional cell carcinoma with incomplete surgical margins. Tumour regrowth was suspected when dysuria was found approximately 318 days after surgery. Clinical signs were palliated by radiation using weekly fractions of 6 Gy for three weeks. The cat died of unknown causes 386 days postoperatively.
  • S Takagi, Y Kato, K Asano, T Ohsaki, D Bosnakovski, Y Hoshino, M Okumura, T Kadosawa, T Fujinaga
    JOURNAL OF VETERINARY MEDICAL SCIENCE 67 8 761 - 767 2005年08月 [査読有り][通常論文]
     
    Matrix metalloproteinases (MMPs) selectively degrade the extracellular matrix, and they have been reported to play an important role in tumor invasion, metastasis and angiogenesis. These enzymes are closely related to tumor malignancy and patient survival time. Recently, reversion-inducing cysteine-rich protein with Kazal motifs (RECK) gene was identified as an endogenous membrane-anchored MMP inhibitor. The down-regulation of RECK has been implicated in tumor progression. In this study, the expression levels of the RECK messenger ribonucleic acid (mRNA) in various spontaneously developed canine tumors were investigated by using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), and the correlation between RECK and clinicopathological factors, as well as MMP-9 expression were analyzed. The median age of 36 dogs investigated in this study was 9 years old (range, 1-15 years old). Quantitative RT-PCR could detect low levels of expression of RECK mRNA in the tumor samples. The expression levels of RECK mRNA in some tumor tissue samples were significantly lower than those in normal tissue samples. No significant associations of RECK with clinicopathological factors were observed. Using the Mann-Whitney U test, the expression level of the MMP-9 mRNA was observed to be significantly correlated to RECK expression (P < 0.05).
  • H Kusuhara, T Hohdatsu, M Okumura, K Sato, Y Suzuki, K Motokawa, T Gemma, R Watanabe, CJ Huang, S Arai, H Koyama
    VETERINARY MICROBIOLOGY 108 3-4 155 - 165 2005年07月 [査読有り][通常論文]
     
    Fel-O-Vax FIV is a dual-subtype vaccine consisting of inactivated whole viruses of subtype A (Petaluma strain) and subtype D (Shizuoka strain). The efficacy of this vaccine against heterologous subtype A strain challenge was demonstrated, but it is unclear whether the result reflects efficacy in the field. In this study, we evaluated the efficacy of this vaccine against contact challenge by exposing both vaccinated and unvaccinated control animals with cats infected with Aomori-2 strain belonging to subtype B, a subtype prevalent in many regions of the world. Nineteen specific-pathogen-free (SPF) cats were divided into a vaccinated group (six cats), an unvaccinated control group (eight cats), and a challenge group (five cats), and maintained in the same room. Cats were monitored for FIV proviral DNA by nested PCR and for FIV-specific antibody levels by ELISA. After I year of commingling, each cat in the vaccinated group was given a booster dose. In addition, the original challenge group was removed and replaced with another challenge group of SPF cats, which were inoculated with the Aomori-2 strain. FIV infection was confirmed in four of the eight animals in the unvaccinated control group by the 29th week in the second year of commingling. In contrast, all of the animals were negative in the vaccinated group. These findings confirmed the efficacy of this vaccine against heterologous stains classified as subtype B, and suggested that the vaccine exhibits broad efficacy against genetically diverse FIV. (c) 2005 Elsevier B.V. All rights reserved.
  • Satoshi Takagi, Tsuyoshi Kadosawa, Tomohiro Ohsaki, Yuki Hoshino, Masahiro Okumura, Toru Fujinaga
    Journal of the American Veterinary Medical Association 226 8 1359 - 63 2005年04月15日 [査読有り][通常論文]
     
    A 6-month-old female Border Collie was examined because of a 1-month history of progressive curvature of the cervical portion of the vertebral column. Radiography revealed severe cervical and thoracic scoliosis. Cervical syringomyelia and hydrocephalus were observed by means of magnetic resonance imaging. Suboccipital craniotomy and laminectomy of the first cervical vertebra were performed, and substantial improvement in the scoliosis and syringomyelia was observed 3 months after surgery. No recurrences were seen during the first year after surgery.
  • S Takagi, T Kitamura, Y Hosaka, T Ohsaki, D Bosnakovski, T Kadosawa, M Okumura, T Fujinaga
    JOURNAL OF VETERINARY MEDICAL SCIENCE 67 4 385 - 391 2005年04月 [査読有り][通常論文]
     
    The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) gene is one of the endogenous matrix metalloproteinase (MMP) inhibitors. It was reported that decreased RECK expression closely correlated with tumor malignancy. We determined the cDNA sequence of the canine RECK gene. The cDNA sequence and deduced amino acid of canine RECK were 2,913 bases and 971 residues, respectively. The predicted amino acid sequence of the protein showed 95.5% and 91.9% homology with human and mouse RECK, respectively. RECK mRNA expression was analyzed in various canine tissues and tumor cell lines by quantitative RT-PCR. The highest RECK expression was detected in lung and testis. In comparison with the tissues, a remarkably low expression level was detected in tumor cell lines. In addition, the RECK gene was transfected in the canine transitional cell carcinoma, and its influence on cell proliferation. migration, and invasion was analyzed. The transfected RECK gene suppressed only canine tumor invasion. These results showed that RECK might play an important role in tumor malignancy in dogs as well as in other mammalians.
  • Darko Bosnakovski, Morimichi Mizuno, Gonhyung Kim, Satoshi Takagi, Masahiro Okumura, Toru Fujinaga
    Cell and tissue research 319 2 243 - 53 2005年02月 [査読有り][通常論文]
     
    The bone marrow harbors a population of mesenchymal stem cells (MSCs) that possess the potential to differentiate into bone, cartilage, and fat, and along other tissue pathways. To date, MSCs from various species have been studied. Despite the bovine experimental model being widely used in experiments in vivo and in vitro, only a limited amount of information regarding bovine MSCs is available. The aim of this study was to isolate and induce the multilineage mesenchymal differentiation of bovine MSCs, thereby initiating further research on these cells. Bovine MSCs were isolated from eight calves, and osteogenic, chondrogenic, and adipogenic differentiation was induced by using a combination of previously reported protocols for other species. The level of differentiation was evaluated by histological examination and by analyzing the expression of tissue-specific genes by a quantitative "real time" reverse transcription/polymerase chain reaction technique. Following osteoinduction, the isolated fibroblast-like cells transformed into cuboidal cells and formed alkaline-phosphatase-positive colonies; during differentiation, these colonies transformed into mineralized nodules. In addition, osteogenesis was followed by osteocalcin and collagen type I mRNA expression. Chondrogenesis was confirmed by the demonstration of collagen type II, aggrecan, and sox9 mRNA expression in the cells stimulated by transforming growth factor beta1 in monolayer culture. After being cultured in an adipogenesis-inducing medium, the MSCs responded by the accumulation of lipid vacuoles and the expression of adipocyte-specific genes. We have therefore demonstrated that cells harvested from bovine bone marrow are capable of in vitro extensive multiplication and multilineage differentiation, making them a relevant and invaluable model in the field of stem cell research.
  • K Ishioka, M Okumura, M Sagawa, F Nakadomo, K Kimura, M Saito
    VETERINARY RADIOLOGY & ULTRASOUND 46 1 49 - 53 2005年01月 [査読有り][通常論文]
     
    Obesity is the most common nutritional disorder in small animals. To establish a computed tomogmphic (CT) method for assessment of visceral and subcutaneous fat content in the dog, CT analysis was performed in normal and obese beagles. Fat area was measured by the level detection method at varied attenuation ranges and compared with body fat content estimated by the deuterium oxide dilution method. Fat area measured at L3 using the attenuation range of -135/-105 Hounsfield unit had the best correlation with body fat content (r=0.98). Regional fat distribution was almost the same between normal and obese dogs with more fat accumulation at L1-S1 than T10-T13. Moreover, visceral and subcutaneous fat area could be estimated separately. This CT method may contribute to both the clinical diagnosis and the study of canine obesity. especially for studies in the relationship between body fat distribution and obesity-associated diseases.
  • Takashi Mori, Masaaki Murakami, Masahiro Okumura, Tsuyoshi Kadosawa, Toshimitsu Uede, Toru Fujinaga
    The Journal of veterinary medical science 67 1 51 - 6 2005年01月 [査読有り][通常論文]
     
    In order to analyze the detailed mechanisms responsible for macrophage activation by chitin derivatives, resident peritoneal macrophages were prepared and stimulated with chitin, chitosan and low-molecular weight chitosan. Our findings were as follows: (i) chitosan induced apoptosis of peritoneal macrophages, but this did not occur when chitin or water soluble low-molecular weight chitosan were used; (ii) chitosan treatment induced activation markers, such as the major histocompatibility complex (MHC) class I, class II, Fc receptors, transferrin receptor, mannose receptor, Fas, and macrophage inflammatory protein (MIP)-2, whereas chitin and low molecular weight soluble chitosan induced only the expression of MHC class I and II molecules; (iii) apoptosis induced by chitosan was mediated by the Fas signaling pathway, in response to phagocytosis via the mannose receptor. We conclude that since chitosan activates macrophages, this may be the mechanism by which it accelerates wound healing.
  • Kazuhiro Watanabe, Tsuyoshi Kadosawa, Taketo Ishiguro, Satoshi Takagi, Kenji Ochiai, Takashi Kimura, Masahiro Okumura, Toru Fujinaga
    The Journal of veterinary medical science 66 9 1167 - 70 2004年09月 [査読有り][通常論文]
     
    Odontogenic cysts, which showed cystic radiolucency in the jaw bone by radiographic examination and computed tomography, were enucleated by operation in 3 dogs. One dog had a odontogenic keratocyst in the incisive bone of the right maxilla and another 2 cases revealed dentigerous cysts in the mandible. These cyst walls were enucleated or transpired by semiconductor laser. Afterwards, osteogenesis was confirmed at the defective part of jaw bone by extirpation of the cyst in all cases, and no recurrence has been noted in any cases. Odontogenic cyst is a disease which should be treated by surgical extirpation or transpiration.
  • Darko Bosnakovski, Morimichi Mizuno, Gonhyung Kim, Taketo Ishiguro, Masahiro Okumura, Toshihiko Iwanaga, Tsuyoshi Kadosawa, Toru Fujinaga
    Experimental hematology 32 5 502 - 9 2004年05月 [査読有り][通常論文]
     
    OBJECTIVE: Pluripotent mesenchymal stem cells (MSC) have been isolated and well characterized from several tissue sources, including bone marrow stroma. MSC from different animals showed slight differences in morphology and in the potential to differentiate. In the present study, we isolated MSC from bovine bone marrow and induced chondrogenesis in order to establish a new experimental model of stem cell research. METHODS: Bone marrow was harvested from 8 calves. For inducing chondrogenesis, MSC were cultured in pellet culture system in a chemically defined medium supplemented with 0 and 10 ng/mL of transforming growth factor beta1 (TGF-beta1). Chondrogenic differentiation was evaluated by histological, immunohistochemical, and in situ hybridization techniques. The degrees of genes expression were measured by quantitative RT-PCR. RESULTS: Metachromatic alcian blue staining and immunoreactivity for type II collagen were detected in both pellet groups (0 and 10 ng/mL TGF-beta1) after 7 days of culturing. In situ hybridization demonstrated strong expression of type II collagen and aggrecan mRNAs in the round cells located at the center region of pellets and at densely organized areas. On the other hand, type I collagen mRNA was strongly expressed in the superficial layer of the pellets. After 20 days of pellet culture, expression of type II collagen mRNA in the cells which were not treated by TGF-beta1 was 1.7-fold higher compared with that treated by TGF-beta1. CONCLUSION: Independent, spontaneous chondrogenesis of bovine MSC in pellet culture occurred without addition of any external bioactive stimulators, namely factors from TGF-beta family, which were previously considered necessary.
  • Gonhyung Kim, Masahiro Okumura, Darko Bosnakovski, Taketo Ishiguro, Tsuyoshi Kadosawa, Toru Fujinaga
    The Journal of veterinary medical science 66 3 263 - 7 2004年03月 [査読有り][通常論文]
     
    To evaluate the effects of chondrocytes transplantation on the regeneration of cartilage by intraarticular injection or injection into blood clots at cartilage defects, eight full-thickness cartilage defects were created surgically on the articular surface of each femoral trochlea of two calves. Autologous chondrocytes were isolated individually from the cartilage pieces collected at the creation of defects. And isolated cells were cultured in monolayers for proliferation. Cells were injected into synovial fluid (Group 2, n=11) or into the blood clots at the cartilage defects (Group 3, n=5) of the left femoropatellar joint on weeks 2 and 3, respectively after the operation. The defects (Group 1, n=16) of right femoropatellar joint were left untreated in the control group. After 14 weeks, repaired tissues were evaluated based on gross and histological examinations. In Group 3, more repaired tissues and a better interface between the repaired tissue and host cartilage were observed compared with the results for Groups 1 and 2. Moreover, cartilaginous tissue were observed more in defects of Group 3 than in defects of other groups. In conclusion, the present study suggests that the injection of cells into the blood clot at a cartilage defect might be applicable for the regeneration of damaged cartilage.
  • G Kim, M Okumura, D Bosnakovski, T Ishiguro, T Kadosawa, T Fujinaga
    Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 50 8 418 - 23 2003年10月 [査読有り][通常論文]
     
    Bovine cartilage explants were co-cultured with or without allogenic chondrocytes for 4 weeks. The attachment of the applied chondrocytes to cartilage after labelling with fluorescence was assessed using a confocal laser microscope. Morphological changes and the production of extracellular matrix (ECM) of co-cultured chondrocytes on intact and damaged surfaces of cartilage were evaluated by histological and immunohistochemical methods. Co-cultured chondrocytes attached to and proliferated on the intact and damaged areas of cartilage, and a new layer was created there. The defects were also filled with ECM produced by the co-cultured chondrocytes. Glycosaminoglycans and collagen type II were detected in the newly formed ECM, and large numbers of rounded chondrocytes were observed at primitive lacunae in this matrix at 4 weeks of culture. The results suggest that chondrocytes have the ability to attach to, to proliferate on and to establish a new matrix on the intact and damaged surfaces of cartilage explants.
  • Kazuhiro Watanabe, Masahiro Kikuchi, Masahiro Okumura, Tsuyoshi Kadosawa, Toru Fujinaga
    The Journal of veterinary medical science 65 9 1007 - 10 2003年09月 [査読有り][通常論文]
     
    Enamel matrix protein (EMP) was applied for regeneration of periodontal tissue in 2 dogs with spontaneous periodontal disease. Case 1 had bony resorption around the root and root apex of the maxillary fourth premolars. Case 2 had vertical resorption of bone between the mandibular first and second molars. A flap was formed in the buccal gingiva, and EMP was applied onto the surface of the exposed root. One or 4 months postoperatively, increased bone level and clinical attachment were recognized. EMP was therefore suggested to be effective to induce regeneration of periodontal tissues in the cases with periodontal disease.
  • Gonhyung Kim, Masahiro Okumura, Darko Bosnakovski, Taketo Ishiguro, Chun-Ho Park, Tsuyoshi Kadosawa, Toru Fujinaga
    The Japanese journal of veterinary research 51 2 83 - 94 2003年08月 [査読有り][通常論文]
     
    Bovine chondrocytes were cultured in monolayers and alginate beads with or without ascorbic acid (Asc) for 16 days. Cell proliferation was examined every 4 days by staining with Hoechst 33258 dye. The gene expression of aggrecan, and collagen type I and II was analyzed at 16 days by reverse transcription and polymerase chain reaction. Cell morphology and the production of extracellular matrix (ECM) were evaluated by cytochemical, immunocytochemical and electron microscopical methods. Cells were continuously cultured in alginate beads with Asc for 2 months, and the cell morphology and ECM were examined. The proliferation of chondrocytes was significantly stimulated with Asc in both monolayers and alginate beads at 16 days. Expression of the collagen type I gene in both cultures was increased, and that of the collagen type II gene in alginate beads was decreased, by Asc. There were no significant cytochemical and immunocytochemical differences between the cultures in alginate beads with or without Asc at 16 days. In alginate beads cultured with Asc for 2 months, proliferating cells were observed mainly at the periphery of the beads, and glycosaminoglycan and collagen type II were found around the cells. These results suggest that Asc stimulated the proliferation of chondrocytes and maintained the chondrogenic properties of the cells in an alginate beads culture.
  • Gonhyung Kim, Masahiro Okumura, Taketo Ishiguro, Tsuyoshi Kadosawa, Toru Fujinaga
    The Journal of veterinary medical science 65 3 427 - 30 2003年03月 [査読有り][通常論文]
     
    Attachment and migration of bovine chondrocytes cultured in vitro were significantly suppressed by the addition of interleukin (IL)-1alpha at the concentration of 1 ng/ml or more (p<0.05). The application of hyaluronic acid (HA) at the concentration of 10 micro g/m l or more significantly recovered the attachment of chondrocytes (p<0.05) and the application of HA at 100 micro g/ml concentration recovered the migration of chondrocytes suppressed by IL-1alpha. These results suggest that the application of HA for inflammatory arthropathies or chondrocyte transplantation might be helpful to preserve the properties of chondrocytes and its extracellular matrix against inflammatory conditions.
  • T Ishiguro, T Kadosawa, S Takagi, G Kim, T Ohsaki, D Bosnakovski, M Okumura, T Fujinaga
    JOURNAL OF VETERINARY INTERNAL MEDICINE 17 2 194 - 198 2003年03月 [査読有り][通常論文]
     
    Plasma histamine concentrations (PHCs) were measured serially over 9 months or until death in I I dogs with mast cell tumors (MCTs). Eight dogs had grossly visible disease and the other 3 dogs had microscopic disease. Initial PHCs in the dogs with gross disease were significantly higher than PHCs in healthy dogs (median, 0.73 ng/mL and 0.19 ng/mL respectively; P < .009), whereas initial PHCs in dogs with microscopic disease showed no difference from controls. Seven dogs subsequently had progressive increases in PHC, and developed hyperhistaminemia (median, 14.0 ng/mL; range, 5.11-30.1 ng/mL). These 7 dogs died from MCTs, and I had general weakness with rapid lysis of a large tumor burden after radiation therapy. PHCs of the other 4 dogs were less than I ng/mL during the study. These 4 dogs were still alive with adequate control of the tumor at the conclusion of the study. Four of the I I dogs initially had gastrointestinal (GI) signs, which abated soon after administration of histamine-2 (H-2) blockers. No significant difference was found between PHCs in dogs with GI signs and those without GI signs (median, 0.86 ng/mL and 0.35 ng/mL, respectively). Thereafter, 7 dogs had serious GI complications for which H-2 blocker therapy was ineffective. PHCs in these 7 dogs were extremely high (median, 12.2 ng/mL; range, 3.42-30.1 ng/mL). Results of this study demonstrated that PHC was one factor related to disease progression, and indicated that marked hyperhistaminemia was associated with the GI signs refractory to H-2 blocker therapy in dogs with MCTs.
  • Junko Nio, Ayaka Yokoyama, Masahiro Okumura, Toshihiko Iwanaga
    Archives of histology and cytology 65 2 189 - 200 2002年06月 [査読有り][通常論文]
     
    The synovial intima is composed of two types of synoviocytes: absorptive macrophages and secretory, fibroblast-like F cells. Many studies have tried to observe synoviocytes by scanning electron microscopy (SEM) but failed to reveal the entire shape of synoviocytes because they are deeply embedded in the interstitial matrix. The present study, primarily employing SEM observation of NaOH macerated samples, reveals the distribution and three-dimensional ultrastructure of the synoviocytes in the normal knee joint of rabbits, and the morphological changes of synoviocytes in an osteoarthritis model of this animal. F cells were broadly distributed throughout the synovial intima, while macrophages showed a restricted distribution on fatty tissues around the patella. F cells were classified into a flat type, which covered the surface of synovial membrane like an epithelium, and a dendritic type, which extended long processes to form a characteristic meshwork on the surface. The flat type predominated in regions adhering to the femur, while the dendritic type predominated in ambilateral parts of both the patella and tendon of the musculus quadriceps femoris, and on the peripatellar fatty tissue. Intermediate forms of flat and dendritic types appeared in middle regions between the patella and periphery of the joint capsule. In the synovial membrane of the osteoarthritis model, both types of synoviocytes increased in number and changed their morphology, indicating their elevated activities in absorption and secretion. It is suggested that the ultrastructural changes in synoviocytes reflect pathological conditions of the synovial membrane, and synoviocytes play important roles in the pathogenesis of osteoarthritis.
  • H Ueno, T Kadosawa, H Isomura, Y Okada, K Ochiai, T Umemura, M Okumura, T Fujinaga
    The Journal of small animal practice 43 5 217 - 20 2002年05月 [査読有り][通常論文]
     
    A perianal rhabdomyosarcoma was diagnosed in an 11-year-old neutered male Labrador retriever. Following two incomplete surgical excisions of the tumour, the dog was treated by means of surgery combined with local radiotherapy and systemic chemotherapy using one cycle of vincristine sulphate, doxorubicin and cyclophosphamide (VAC protocol). The dog died 252 days after the first combined therapy. Radiography at this time demonstrated enlargement of the iliac lymph nodes, suggesting metastasis of the tumour. To the authors' knowledge, this is the first report of treatment of canine perianal rhabdomyosarcoma.
  • M Okumura, G H Kim, M Tagami, S Haramaki, T Fujinaga
    Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 49 4 195 - 7 2002年05月 [査読有り][通常論文]
     
    Keratan sulphate (KS) concentration in sera from resting horses and horses training daily on a racetrack was measured by an inhibition enzyme-linked immunosorbent assay (ELISA) using anti-equine KS antibody 1/14/16H9. For the in-training horses, serum KS concentrations in 2-year-old-horses was significantly higher than 3- or 4-year-old-horses. A higher concentration of serum KS was found in the in-training group than in the long-term resting group in 2-year-old-horses. Serum KS concentration increased remarkably immediately after training in healthy horses, and at 1, 5, 9 and 24 h after training remained at similar levels to the pre-training concentration. The results suggest that serum KS concentration could represent the situation of joint loading, induced by daily racetrack training, affecting the metabolic activities in joint cartilage.
  • イヌの肥満に対する魚油給餌の効果とUCPの発現
    石岡克己, Mohamed Mohamed Soliman, 奥村正裕, 佐川真由美, 柴田治樹, 本庄 勉, 北村 浩, 木村和弘, 斉藤昌之
    獣医生化学 37 19 - 26 2002年 [査読有り][通常論文]
  • T Ishiguro, T Kadosawa, K Mori, S Takagi, M Okumura, T Fujinaga
    The Journal of veterinary medical science 63 9 1031 - 4 2001年09月 [査読有り][通常論文]
     
    A new cell line (CoMS) was established from a 3-year-old male mongrel dog with mast cell tumor of the oral mucosa. CoMS cells grow in suspension with a doubling time of 27.0 +/- 0.7 hr. The cytoplasmic granules were formalin-sensitive, showed diverse appearances in their ultrastructural findings and contained heparin proteoglycan and neutral protease chymase. Calcium ionophore A23187, substance P and concanavalin A caused significant histamine release from CoMS cells, while compound 48/80 failed to release histamine. This cell line will make an available source for studies on canine mast cell tumors.
  • H Ueno, F Nakamura, M Murakami, M Okumura, T Kadosawa, T Fujinag
    Biomaterials 22 15 2125 - 30 2001年08月 [査読有り][通常論文]
     
    Chitosan is reported as an accelerator of wound healing. Histological findings of previous reports indicate that chitosan accelerates the reformation of connective tissue, however the details of the mechanism are not clear. In this study, firstly L929 mouse fibroblasts were cultured with chitosan and the production of extracellular matrix (ECM) was evaluated in vitro. Type I and III collagens and fibronectin were secreted by L929 with or without chitosan; however there was no significant difference in the amount of ECM between the control and the chitosan groups. Secondly, macrophages were stimulated with chitosan, and then transforming growth factor-beta 1 (TGF-beta1) and platelet-derived growth factor (PDGF) messenger ribonucleic acid (mRNA) expressions and production of their proteins were assayed in vitro. As a result, chitosan promoted the production of TGF-beta1 and PDGF. These results indicate that chitosan does not directly accelerate ECM production by fibroblast and the ECM production may increase by the growth factors.
  • K Watanabe, M Kikuchi, M Okumura, T Kadosawa, T Fujinaga
    The Journal of veterinary medical science 63 8 889 - 94 2001年08月 [査読有り][通常論文]
     
    Adult dogs have a complex apical delta structure in all root apexes of teeth. This complex structure may affect the formation of apical lesions in the teeth such as apical abscesses. The purpose of this study was to evaluate the efficacy of enamel matrix protein (EMP) which was used for periodontal regeneration therapy after an experimental apicoectomy for an assumed apical lesions of the teeth in dogs. The maxillar canine roots and maxillar fourth premolar buccal mesial roots in five beagles were experimentally apicoectomized under general inhalation anesthesia. After the root apex was exposed and excised, EMP was applied on the surface of the exposed dentin. After 12 weeks, dogs were euthanized. and the experimental teeth together with the surrounding soft and hard periodontal tissues were collected for histological evaluation under a light microscope. In the EMP group, the size of the defect where the root apex was removed was smaller than that of the control group. New cementum was dominantly achieved in the EMP group compared to the control group. Furthermore, new collagen fibers that bridged area between the new cementum and new alveolar bone were detected only in the EMP group. The present results demonstrated marked apical periodontal regeneration after apicoectomy in the EMP group. These results, therefore, suggest that the application of EMP can effectively induce the regeneration of periodontal strUctures in apicoectomized dogs.
  • H Kitamura, M Okumura, F Sato, K Kimoto, M Kohama, Y Hashimoto, M Tagami, T Iwanaga
    The Japanese journal of veterinary research 49 2 115 - 23 2001年08月 [査読有り][通常論文]
     
    Our previous study established protein gene product 9.5 (PGP 9.5), a ubiquitin C-terminal hydrolase, as a specific cytochemical marker of synovial lining cells (type B synoviocytes) in the horse joint. The present study aimed to detect PGP 9.5 in the synovial fluid and shows that PGP 9.5 is a valuable marker of osteoarthritis in the horse. Immunohistochemical staining confirmed rich and consistent localization of PGP 9.5 immunoreactivity in the cytoplasm of synovial lining cells in the normal horse joint. Western blot analysis of synovial fluid from normal joints could detect a significant band corresponding to that contained in the brain and synovial membrane extracts. When 60 synovial fluid samples from normal and abnormal joints were assayed with an enzyme-linked immunosorbent assay (ELISA) system, the concentration of PGP 9.5 tended to be elevated in osteochondrosis dissecance, inflammatory arthropathy and intra-articular fracture, among which a statistically significant elevation was recognizable between the intra-articular fracture and the control. Thus, this study demonstrated the possibility that PGP 9.5, derived from synovial lining cells, may be a new biochemical marker for arthritic disorders of the horse.
  • T Mori, T Kadosawa, Y Okada, K Ochiai, T Umemura, S Tanoue, M Okumura, T Fujinaga
    The Journal of small animal practice 42 7 349 - 51 2001年07月 [査読有り][通常論文]
     
    A seven-year-old crossbred male dog with a suspected leukaemic condition was referred for investigation and treatment. A bone marrow aspirate revealed an acute myeloid leukaemia. Combination chemotherapy was administered and the dog initially improved, but 18 days after the initiation of therapy its body condition deteriorated and the animal developed acute respiratory distress. On postmortem examination, extensive leukaemic pulmonary infiltrates were evident.
  • K Watanabe, M Kikuchi, E F Barroga, M Okumura, T Kadosawa, T Fujinaga
    The Journal of veterinary medical science 63 7 789 - 95 2001年07月 [査読有り][通常論文]
     
    To determine the process of formation of apical delta, a histological study on the permanent teeth was carried out in dogs. A litter of 7 clinically healthy beagle dogs and 33 adult dogs (4- to 15- year-old) of 12 breeds with periodontal disease were used for the experiments. Teeth extracted from 6-,7-,8- and 9-month-old beagles were sectioned and stained with HE solution. Tooth roots obtained from adult dogs with periodontal disease were ground. Each tooth was classified into the following root types under a light microscope: Type I (no apical delta = no apical closure), II (few apical delta), IIIA (low apical delta) and IIIB (high apical delta). In the 6-month-old beagles, more than half the tooth roots were classified as type I. In the 7-month-old beagles, type IIIB apical delta was the most predominant and types I, II and IIIA apical delta were occassionally seen. Apical closure and delta were observed in all beagles at 8 months of age histologically. In the 8- and 9-month-old beagles, all root apexes observed were type IIIB. Most of the 314 tooth roots extracted from 33 adult dogs were type IIIB, but a few were type IIIA.
  • H Ueno, M Murakami, M Okumura, T Kadosawa, T Uede, T Fujinaga
    Biomaterials 22 12 1667 - 73 2001年06月 [査読有り][通常論文]
     
    Chitosan is a copolymer of beta(1 --> 4) glucosamine and N-acetyl-D-glucosamine, which accelerates the infiltration of polymorphonuclear leukocytes (PMN) in the early phase of wound healing. In the granulation tissue treated with chitosan in canine experimental wound, osteopontin (OPN) was strongly positive in PMN immunohistochemically. OPN is a glycosylated phosphoprotein and promotes the attachment or spread of a variety of cell types. In addition, OPN may play a role in granulomatous inflammation. Production of OPN in PMN was therefore investigated in vitro using human PMN in this study. PMN stimulated with granulocyte-colony stimulating factor (G-CSF) and chitosan accumulated OPN mRNA, and released OPN into their culture supernatants. These findings suggest that OPN is synthesized by migrating PMN which plays the novel role of regulating the evolution of wound healing with chitosan treatment at the early phase of healing.
  • K Asano, K Masuda, M Okumura, T Kadosawa, T Fujinaga
    The Journal of veterinary medical science 63 3 243 - 50 2001年03月 [査読有り][通常論文]
     
    Association between exogenous atrial natriuretic peptide (ANP) and hemodynamic changes was ascertained in 3 dogs with overt congestive heart failure (CHF(+)) and 3 dogs without congestive heart failure (CHF(-)) caused by experimental mitral regurgitation (MR). The hemodynamic measurements were recorded in all dogs during and after 1 hr infusion of ANP at the rate of 0.1 (low dose), 0.5 (medium dose) and 1.0 (high dose) microg/kg/min, respectively. Heart rate, mean arterial pressure, pulmonary capillary wedge pressure (PCWP) and systemic vascular resistance decreased significantly during and after ANP infusion even with low dose in the CHF(+). Stroke volume, stroke volume index and cardiac output in the CHF(+) during and after ANP infusion showed an increasing trend as compared with the CHF(-). Double product, an indicator of myocardial oxygen consumption, significantly decreased during and after ANP administration at all doses in the CHF(+). These findings indicate that even at low dose, exogenous ANP improves cardiac performance and reduces myocardial oxygen consumption in the CHF(+), and suggest that ANP has beneficial effects in the treatment of dogs with overt congestive heart failure resulting from MR.
  • E F Barroga, T Kadosawa, M Okumura, T Fujinaga
    Veterinary journal (London, England : 1997) 159 2 186 - 93 2000年03月 [査読有り][通常論文]
     
    The efficacy of 22-oxacalcitriol (OCT), calcitriol, cholecalciferol, all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9-cis RA) to differentiate in vitro four clonal cells of the canine osteosarcoma cell line POS into cells having properties of a functionally mature osteoblast bone cell were investigated. The induction of intracellular alkaline phosphatase (ALP) activity, osteocalcin (GLA-OC) and type I collagen (PIP) production after 72 h treatment were used as markers of differentiation. At a concentration of 10(-8)M, OCT and calcitriol significantly induced all markers, and ATRA only the ALP of osteoblast, chondroblast and undifferentiated clonal cells. At the same concentration, 9-cis RA and cholecalciferol induced ALP of chondroblast and osteoblast cells, respectively; ATRA, 9-cis RA and cholecalciferol induced PIP of chondroblast and undifferentiated cells. None of the drugs significantly differentiated fibroblast cells. The ability of these agents to differentiate osteosarcoma cells into cells that exhibit properties of functionally mature osteoblastic bone cells may promote normal osteogenesis and reverse the loss of control of their differentiation.
  • M Okumura, M Tagami, T Fujinaga
    The Journal of veterinary medical science 62 3 281 - 5 2000年03月 [査読有り][通常論文]
     
    The role of keratan sulphate (KS) as a marker of cartilage metabolism was evaluated by using an in vitro model of equine articular cartilage. Articular cartilage was harvested from clinically healthy 6-month-old foals (n=3). Chondrocytes were centrifuged and cultured as pellets. Chondrocyte pellets were stimulated by insulin-like growth factor (IGF)-Ialpha or interleukin (IL)-1alpha for 2 weeks. The sulfated glycosaminoglycans (GAG) and antigenic KS concentrations in the culture media were measured by a 1,9-dimethyl-methylene blue (DMMB) colorimetric assay and an inhibition ELISA using a 1/14/16H9 antibody, respectively. Concentration of GAG was significantly increased in the media of pellets stimulated by both IGF-Ialpha and IL-1alpha. Antigenic KS concentration was significantly increased in those stimulated by IL-1alpha, while no significant change was found in those stimulated by IGF-Ialpha. A high correlation between GAG and antigenic KS concentrations was found in the media of pellets stimulated by IL-1alpha (r=0.87), but not in those stimulated by IGF-Ialpha (r=0.43). The results suggest that the concentration of antigenic KS reacting to 1/14/16H9 mirrors the GAG concentration during the stage of cartilage catabolism, but not during the cartilage anabolic stage. The concentration of antigenic KS reacting to 1/14/16H9 antibody in biological fluids could therefore be a useful marker to further understand principally the catabolic and slightly the anabolic process of articular cartilage metabolism.
  • M Okumura, T Fujinaga
    Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 47 2 99 - 105 2000年03月 [査読有り][通常論文]
     
    The role of keratan sulphate (KS) as a metabolic marker of cartilage was evaluated using an in vitro model of equine articular cartilage. Articular cartilage was harvested from clinically healthy 6-month-old foals (n = 3). Chondrocytes were centrifuged and cultured as pellets. Chondrocyte pellets were stimulated by insulin-like growth factor-I alpha (IGF-I alpha) or interleukin-1 alpha (IL-1 alpha) for 2 weeks. The concentrations of sulphated glycosaminoglycans (GAG) and KS in the culture media were measured by a 1,9-dimethyl-methylene blue (DMMB) colorimetric assay and an inhibition enzyme-linked immunosorbent assay using a 1/20/5D4 antibody, respectively. The concentration of GAG was significantly increased both in the media of pellets stimulated by IGF-I alpha and in those stimulated by IL-1 alpha. KS concentration was significantly increased in those stimulated by IL-1 alpha, while no significant change was found in those stimulated by IGF-I alpha. A high correlation between GAG and KS concentrations was found in the media of pellets stimulated by IL-1 alpha (r = 0.84), but not in those stimulated by IGF-I alpha (r = 0.59). The results suggest that the concentration of KS reacting to 1/20/5D4 mirrors the GAG concentration during the stage of cartilage catabolism, but not during the cartilage anabolic stage. The KS concentration in biological fluids could therefore be a useful marker to understand further the cartilage catabolic process. It may also represent some aspects of the cartilage anabolic process.
  • E F Barroga, T Kadosawa, M Okumura, T Fujinaga
    Research in veterinary science 68 1 79 - 87 2000年02月 [査読有り][通常論文]
     
    Pulmonary metastasis is a major cause of death and a major obstacle to the successful treatment of canine osteosarcoma. However, the residual capacity of the neoplasia for differentiation and its susceptibility to undergo apoptosis may be used to suppress its growth and metastatic properties. The highly metastasizing POS (HMPOS) canine osteosarcoma cell line which preferentially metastasize to the lungs was used to test the possible efficacy of 22-oxa-calcitriol (OCT) and all- trans retinoic acid (ATRA) to inhibit growth and pulmonary metastasis of the subcutaneously grown osteosarcoma in nude mice. Treatments in vitro, morphologically elongated and increased alkaline phosphatase activity and staining of cells. Tumour growth in vivo was inhibited significantly and the combination treatment of OCT and ATRA (OCT + ATRA) exerted a synergistic and stronger suppression at concentration of 1.0 microg kg(-1)body weight when given subcutaneously three times a week for 5 weeks. The subcutaneous tumours of the control mice consisted of osteoblast-like cells and isolated chondroblast-like cells, but formed several areas of osteoid and increased amount of collagen tissue in all treated mice. Pinpoint macrometastatic nodules developed only in all control mice. Micrometastatic nodule developed only in two of six mice treated with ATRA. However, nodule size and number, and lung wet weight were all reduced significantly. Metastasis were not seen in the mice treated with OCT or OCT + ATRA. This study demonstrated that inhibition of growth and pulmonary metastasis was induced by subcutaneous treatment with these drugs and suggest that both its differentiating and apoptotic inducing activities may be responsible for the antitumour effects. These drugs may be useful in the clinic as an adjunct for the treatment of canine osteosarcoma.
  • M Okumura, K Watanabe, T Kadosawa, T Fujinaga
    Australian veterinary journal 78 2 95 - 8 2000年02月 [査読有り][通常論文]
     
    Comminuted metatarsal fractures in a dog were treated using intramedullary pins externally connected and stabilized by epoxy resin putty and application of a fibreglass cast. Two weeks later the cast was removed. After further 2 weeks application of a Robert-Jones bandage the dog could stand and walk with the pin-putty apparatus in position. The pin-putty apparatus was removed 3 months postoperatively, and the dog resumed normal activity. This fixation method could be an easy, economical and effective alternative treatment for managing comminuted fractures of the metatarsal and possibly metacarpal bones in selected cases.
  • E Barroga, T Kadosawa, M Okumura, T Fujinaga
    Zentralblatt fur Veterinarmedizin. Reihe A 46 9 573 - 9 1999年11月 [査読有り][通常論文]
     
    The effects of 22-oxacalcitriol (OCT), calcitriol and all-trans retinoic acid (ATRA) on the induction of functional differentiation and growth inhibition of the canine osteosarcoma cell line POS were investigated in vitro via bone differentiation markers and proliferation assays, respectively. The intracellular alkaline phosphatase (ALP) activity and the gamma-carboxyglutamic acid osteocalcin (GLA-OC) and procollagen type I C peptide (PIP) production were used as markers of differentiation. Treatment with 10(-8) M concentrations of all drugs for 72 h significantly inhibited growth (P < 0.0001) and increased ALP activity and GLA-OC and PIP production in POS. OCT, calcitriol and ATRA significantly increased the: ALP activity from 1.58 +/- 0.14 mumol/min/mg protein (mean +/- SD; control) to 2.50 +/- 0.09 (P < 0.0001), 2.30 +/- 0.14 (P < 0.0001) and 2.00 +/- 0.14 (P = 0.0008), respectively; GLA-OC production from 0.71 +/- 0.01 ng/ml (control) to 2.87 +/- 0.01 (P < 0.0001), 2.87 +/- 0.11 (P < 0.0001) and 1.36 +/- 0.06 (P < 0.0001), respectively; and PIP production from 433.91 +/- 23.29 ng/ml (control) to 536.54 +/- 15.46 (P = 0.0002), 497.06 +/- 1.99 (P = 0.0028) and 481.66 +/- 0.01 (P = 0.0104), respectively. This study demonstrated that treatment with these drugs induced a phenotypic maturation of POS cells into cells that exhibit the properties of functionally mature bone cells with parallel growth inhibition. The effects of these drugs on functional differentiation may be useful to complement the progression of a normal osteogenic differentiation process in the sarcoma.
  • H Ueno, H Yamada, I Tanaka, N Kaba, M Matsuura, M Okumura, T Kadosawa, T Fujinaga
    Biomaterials 20 15 1407 - 14 1999年08月 [査読有り][通常論文]
     
    Chitosan is a polymeric beta(1 --> 4) glucosamine (2-amino-2-deoxy-D-glucose) and N-acetyl-D-glucosamine (2-acetamido-2-deoxy-D-glucose) which has been reported as a wound healing accelerator. In order to evaluate the efficacy of chitosan as an accelerator of wound healing, experimental open skin wounds were made on the dorsal side in three normal beagles. Cottonfiber-type chitosan (degree of acetylation = 18%) was applied for 15 days, and the process of wound healing was evaluated histologically and immunohistochemically. On day 3 postwounding, the chitosan-treated wounds showed histologically severe infiltration of polymorphonuclear (PMN) cells and an increase in effusion compared with that in the control. Granulation was more pronounced by the chitosan treatment on day 9 and 15 postwounding. Immunohistochemical typing of collagen I, III and IV showed increase of the production of type III collagen in the chitosan group. The appearance of mitotic cells occurred numerously in the control on postwounding day 3, and in the chitosan group on postwounding day 6. These results suggest chitosan to be having a function in the acceleration of infiltration of PMN cells at the early stage of wound healing, followed by the production of collagen by fibroblasts.
  • E F Barroga, T Kadosawa, M Okumura, T Fujinaga
    Research in veterinary science 66 3 231 - 6 1999年06月 [査読有り][通常論文]
     
    Although canine osteosarcoma is one of the most malignant, aggressive and lethal neoplasms originating from undifferentiated bone cells, it may retain some capacity for normal differentiation. The purpose of this study was to ascertain if the residual capacity for differentiation could be used to suppress its malignant properties. We tested the efficacy of vitamin D and retinoids in inducing differentiation and inhibiting growth of the POS canine osteosarcoma and four of its clonal cell lines, POS 14A (fibroblast type), POS 53B (chondroblast type), POS 53C (undifferentiated type) and POS 53D (osteoblastic type). Treatment with 10(-10)to 10(-8)M concentrations of calcitriol, OCT, cholecalciferol, all-trans retinoic acid (ATRA) and 9-cis retinoic acid for 48-120 hours changed the morphology of POS, POS 53B, POS 53C and POS 53D cells to cells that were elongated and spindle-shaped. Increased number of cytoplasmic organelles and pronounced nuclear activities were induced by concentrations of 10(-8)M and 10(-7)M for 120 hours. All drugs at concentrations of 10(-10)to 10(-8)M for 72 hours inhibited POS growth dose-dependently. OCT significantly reduced the cell number in all cell lines when used at concentrations between 10(-9)and 10(-8)M for 72 hours and exerted significant anti-proliferative effects for eight days culture. This study demonstrated that changed morphology and inhibition of growth was induced by treatment of the cells with these vitamins, that the loss of control of differentiation in the neoplasia was not irreversible and that these drugs may be useful in the clinic.
  • K Asano, K Masuda, M Okumura, T Kadosawa, T Fujinaga
    The Journal of veterinary medical science 61 5 523 - 9 1999年05月 [査読有り][通常論文]
     
    Plasma concentrations of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were measured in 6 dogs with experimental mitral regurgitation (MR) and 19 canine patients with asymptomatic and symptomatic congestive heart failure (CHF). In dogs with experimental MR, ANP and BNP concentrations were significantly correlated with pulmonary capillary wedge pressure (PCWP) (ANP; r=0.852, P=0.0004, BNP; r=0.832, P=0.0008). ANP level was shown to have a predominant effect on PCWP in comparison with BNP using multiple regression analysis. In canine patients with asymptomatic and symptomatic CHF, ANP and BNP concentrations were significantly different among the heart failure classes according to the New York Heart Association functional classification (ANP; P=0.0165, BNP; P=0.0005). In addition, ANP and BNP levels in dogs with decompensated heart failure (n=10) significantly increased in comparison with those in dogs with compensated heart failure (n=9). There was however no correlation between ANP and BNP levels in each heart failure class. In conclusion, plasma ANP and BNP levels may become predictors of PCWP and the severity of heart failure in dogs with MR, although further investigations on ANP and BNP levels in more clinical cases are required.
  • E F Barroga, T Kadosawa, M Okumura, T Fujinaga
    The Journal of veterinary medical science 61 4 361 - 7 1999年04月 [査読有り][通常論文]
     
    Highly lung metastasizing model of canine osteosarcoma in nude mice was established from five subcutaneous implantation cycles of lung tumor deposits. The selection of cells with increased metastatic properties from the parent POS canine osteosarcoma cell line recovered medium sized and polygonal Highly Metastasizing POS cells (HMPOS). The doubling time of HMPOS and POS in culture averaged 30 +/- 1.2 hr and 32 +/- 1.3 hr respectively, and their cell growth patterns in vitro were comparable to their in vivo growth patterns. HMPOS cells produced more tumor deposits (> 20 nodules, > 1 -mm in diameter) of various sizes with replacement of lung tissues at 12 weeks after implantation. POS cells produced fewer and smaller lung deposits (< 10 nodules, 1-mm in diameter). Tumor size and number of metastatic tumor deposits showed a regular association. HMPOS cells developed an osteoblastic type of cellular differentiation subcutaneously and in the lungs. HMPOS micrometastasis along the alveolar walls and blood vessels at 4 weeks averaged 6-7 small tumor locus. Each micrometastatic locus contained an average of 5-7 tumor cells, and developed a pleomorphic osteoblastic type of cellular differentiation. An average of 4 macrometastatic nodules could be seen at 6 weeks, composed of an average of 23 tumor cells, 10 nodules at 8 weeks, 12 nodules at 10 weeks and 20 nodules at 12 weeks. These model provides an opportunity for the evaluation of new treatments against canine lung metastatic osteosarcoma in a nude mice model.
  • Edward F. Barroga, Tsuyoshi Kadosawa, Masahiro Okumura, Toru Fujinaga
    Journal of Veterinary Medical Science 61 4 361 - 367 1999年04月 [査読有り][通常論文]
     
    Highly lung metastasizing model of canine osteosarcoma in nude mice was established from five subcutaneous implantation cycles of lung tumor deposits. The selection of cells with increased metastatic properties from the parent POS canine osteosarcoma cell line recovered medium sized and polygonal Highly Metastasizing POS cells (HMPOS). The doubling time of HMPOS and POS in culture averaged 30 ± 1.2 hr and 32 ± 1.3 hr respectively, arid their cell growth patterns in vitro were comparable to their in vivo growth patterns. HMPOS cells produced more tumor deposits (> 20 nodules, > 1-mm in diameter) of various sizes with replacement of lung tissues at 12 weeks after implantation. POS cells produced fewer and smaller lung deposits (< 10 nodules, 1-mm in diameter). Tumor size and number of metastatic tumor deposits showed a regular association. HMPOS cells developed an osteoblastic type of cellular differentiation subcutaneously and in the lungs. HMPOS micrometastasis along the alveolar walls and blood vessels at 4 weeks averaged 6-7 small tumor locus. Each micrometastatic locus contained an average of 5-7 tumor cells, and developed a pleomorphic osteoblastic type of cellular differentiation. An average of 4 macrometastatic nodules could be seen at 6 weeks, composed of an average of 23 tumor cells, 10 nodules at 8 weeks, 12 nodules at 10 weeks and 20 nodules at 12 weeks. These model provides an opportunity for the evaluation of new treatments against canine lung metastatic osteosarcoma in a nude mice model.
  • K Asano, T Kadosawa, M Okumura, T Fujinaga
    The Journal of veterinary medical science 61 1 89 - 91 1999年01月 [査読有り][通常論文]
     
    Peri-operative changes in echocardiographic measurements and plasma levels of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were investigated for 1 month in 3 dogs with patent ductus arteriosus (PDA). Post-operative left ventricular end-diastolic dimention and fractional shortening decreased in all cases. Pre-operatively increased plasma ANP concentrations reduced dramatically after the operation. Peri-operative changes in plasma BNP levels had slightly S-shaped curves in all cases. These observations suggest that post-operative responsiveness of ANP and cardiac function are rapid in comparison with cardiac morphological changes, and BNP has a different pathophysiological significance from ANP in dogs with PDA.
  • M Okumura, T Kadosawa, T Fujinaga
    Australian veterinary journal 77 1 24 - 7 1999年01月 [査読有り][通常論文]
     
    Two cats with temporomandibular joint ankylosis were examined. Difficulty in opening the mouth and malocclusion were associated with trauma and fracture around the zygomatic arch and the condyle of the mandible after an automobile accident. Unilateral condylectomy or aggressive caudal mandibulectomy was performed to improve quality of life. Excision arthroplasty of the temporomandibular joint improved mandibular function and appearance. Some malocclusion remained but had no clinical significance.
  • EF Barroga, T Kadosawa, K Asano, M Okumura, T Fujinaga
    JOURNAL OF VETERINARY MEDICAL SCIENCE 60 11 1269 - 1272 1998年11月 [査読有り][通常論文]
     
    Vitamin D-3: 1-alpha,25(OH)(2)D-3 (calcitriol), 22-oxa-1,25(OH)(2)D-3 (OCT), cholecalciferol (vitamin D-3), and retinoids: all-trans retinoic acid (ATRA) and 9-cis retinoic acid, induced morphological changes in POS canine osteosarcoma cells into elongated, spindle or fibroblast like-shaped cells, and apoptotic like cell death characterized by cell shrinkage, condensation and margination of the nucleus for all drugs at 10(-6)M-10(-9)M after 72 to 120 hr culture. Apoptosis as shown by DNA laddering was induced at 48 hr by all drugs at 10(-6)M, 10(-7)M at 96 hr, 10(-8)M and 10(-9)M at 120 hr respectively. These vitamins are suggested to adjunct antineoplastic agents in canine osteosarcoma therapy by induction of apoptosis.
  • M Okumura, T Fujinaga
    American journal of veterinary research 59 10 1203 - 8 1998年10月 [査読有り][通常論文]
     
    OBJECTIVES: To establish a sensitive and specific monoclonal antibody (MAB) against equine keratan sulfate (KS) and to develop an enzyme immunoassay for measurement of the concentration of KS in serum and synovial fluid from horses. SAMPLE POPULATION: 18 synovial fluid and 48 serum samples were obtained from clinically normal horses and horses with arthritis. PROCEDURE: BALB/c mice were immunized with chondroitinase-ABC-digested proteoglycan monomer from equine joint cartilage, and MAB were raised, using Sp2/O cells as a fusion partner. A competitive ELISA was optimized, using one of the established MAB, and KS concentration in synovial fluid and sera from horses was measured. RESULTS: The high-titer MAB1/14/16H9, which specifically recognizes the epitope on equine KS, was identified. This antibody had no reactivity with chondroitin sulfate and core protein of proteoglycan monomers, hyaluronan, heparin, dermatan sulfate, and heparan sulfate. A competitive ELISA for determination of KS concentration was optimized, using this antibody. Precision data were obtained for the range of 10 to 160 ng/ml. The within- and between-assay coefficients of variation were 10.0 and 12.7%, respectively. CONCLUSIONS: MAB 1/14/16H9 that specifically recognized equine KS was established and was used to develop an enzyme immunoassay for measurement of the concentration of KS in synovial fluid and sera from horses. It is expected that the assay system using MAB 1/14/16H9 will contribute to evaluation of cartilage metabolic activity in horses.
  • M Okumura, M Tagami, T Fujinaga
    Zentralblatt fur Veterinarmedizin. Reihe A 45 8 513 - 6 1998年10月 [査読有り][通常論文]
     
    Keratan sulphate (KS) concentration and anticollagen type II antibody levels were measured in the serum and synovial fluid (SF) of clinically normal horses and horses with osteoarthritis (OA). Serum KS in OA was significantly higher than that in normal horses, while no significant difference was found in KS levels of SF between normal and OA. Assays of antibody to collagen type II showed no significant increase in sera and SF of OA. It was suggested that levels of serum KS would be of value in the pathological detection of OA in the joint, although there was no evidence that the measurable autoimmunity to collagen antigens would reflect the process of OA.
  • M Okumura, M Asano, M Tagami, K Tsukiyama, T Fujinaga
    Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 62 2 122 - 6 1998年04月 [査読有り][通常論文]
     
    Serum concentrations of copper (Cu), zinc (Zn), manganese (Mn), calcium (Ca) and inorganic phosphorus (P), as well as antigenic ceruloplasmin (Cp) and oxidase activity as a functional index for copper metabolism, were measured in 10 foals (5 males and 5 females) and their dams. Samples were harvested from the foals within 1 wk after birth and monthly from 1 to 17 mo of age. Samples were collected from their dams in the perinatal period (monthly from 2 mo before delivery to 5 mo postpartum). Serum oxidase activity, antigenic Cp and Cu in foals were extremely low at 1 wk. Serum Cp had the lowest value of 17.0 +/- 8.0 (mean +/- SD) mg/dL within the 1st wk, then increased rapidly up to 43.7 +/- 5.8 mg/dL at 1 mo, and maintained this level until the 17th mo. Serum Zn in foals had the highest value of 73.2 +/- 13.1 micrograms/dL within 1 wk, then decreased to 38.3 +/- 5.9 micrograms/dL by 17 mo. Serum Mn, Ca and P in mares were almost stable and within established reference ranges for our laboratory in the perinatal period, and these values in foals were also in the normal range. Even on appropriate feeding, serum Cu, Cp and oxidase activity were quite low a few weeks after birth, while a higher proportion of Cp-binding copper was found in the foals. This might be caused by the limited synthesis of ceruloplasmin in this period. These data suggest that newborn foals are in a critical situation of marginal copper status in the early stage of growth.
  • T Mwanza, T Miyamoto, M Okumura, T Kadosawa, T Fujinaga
    The Japanese journal of veterinary research 45 4 199 - 206 1998年02月 [査読有り][通常論文]
     
    The purpose of this study was to evaluate the relationship between the results of laboratory examinations and ultrasonographic findings, especially portal vein hemodynamics in experimentally bile duct ligated dogs. Biliary obstruction was accomplished by surgically occluding the common bile duct in five dogs. All the dogs became visibly jaundiced within 24 hours after surgery. The total protein and albumin/globulin ratio showed a gradual decrease throughout the examination period, while blood urea nitrogen reached its peak in the 6th week and decreased to pre ligation values by the 10th week. Similar trends were noted in the alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and direct and total bilirubin. Total cholesterol and fasting serum bile acid levels rapidly increased after surgery to peak values between the 2nd and 4th week, and then gradually decreased, but still remained high throughout the experiment period. The portal flow volume and velocity significantly (p < 0.05) decreased while only a slight increase was noted in the congestion index after bile duct ligation. The cross sectional area of the portal vein changed insignificantly. Bile duct and gallbladder distention was evident within the 1st week after ligation but there was little change in the echogenicity of the liver parenchyma. The results of this study suggest that the determination of Doppler ultrasound parameters of hepatic hemodynamics, especially the portal vein flow indices, may contribute to a better noninvasive assessment of the canine patient with biliary obstructive disease.
  • T Mori, Y Irie, S I Nishimura, S Tokura, M Matsuura, M Okumura, T Kadosawa, T Fujinaga
    Journal of biomedical materials research 43 4 469 - 72 1998年 [査読有り][通常論文]
     
    The effects of chitin and its derivatives on the proliferation of human umbilical vein endothelial cells (HUVECs) and on the production of cytokines were examined in vitro. Chitin and its derivatives had no effect on the proliferation of cultured HUVECs. N-Sulfonated 70% deacetylated chitin (S-DAC70) stimulated the production of interleukin (IL)-1beta, IL-6, IL-8, and tumor necrosis factor (TNF)-alpha from HUVECs. Compared to S-DAC70, the other materials tested in the present study showed less effect in the stimulation of IL-8 and TNF-alpha production and had no effect in the stimulation of IL-1beta and IL-6 production. These results indicated that S-DAC70 affects HUVECs function but not proliferation.
  • T Mwanza, T Miyamoto, M Okumura, T Kadosawa, T Fujinaga
    The Japanese journal of veterinary research 45 3 153 - 61 1997年11月 [査読有り][通常論文]
     
    The results of liver function tests and ultrasonographical findings were analysed in 7 dogs that were intravenously injected dimethylnitrosamine (DMNA 2 mg/kg body weight) on 2 consecutive days each week for 10 weeks. Typical clinical signs and similar changes in liver enzyme concentrations that develop in dogs with natural cirrhosis were observed in this canine model. Severe anaemia and a significant reduction in the platelet numbers occurred in the dogs that died in the 5th week, while in all the other dogs these parameters decreased slightly. Serum total protein and the albumin/globulin ratio decreased gradually while the alkaline phosphatase, alanine amino transferase, aspartate amino transferase and gamma glutamyl transpeptidase activities increased significantly (p < 0.05) in all dogs after beginning the administration of DMNA. Ultrasound findings of a coarsened and heterogeneous echo pattern with increased echogenicity that are characteristic of canine cirrhosis were noticed at the same time when the changes in liver enzymes became evident. Present results suggest that ultrasonography in conjunction with liver function tests may be useful in the evaluation of experimentally induced liver cirrhosis.
  • T Fujinaga, T Kadosawa, M Okumura
    JAPANESE JOURNAL OF VETERINARY RESEARCH 45 3 174 - 175 1997年11月 [査読有り][通常論文]
  • M Okumura, T Fujinaga, E Urakawa, M Tagami, K Tsukiyama
    American journal of veterinary research 58 8 925 - 9 1997年08月 [査読有り][通常論文]
     
    OBJECTIVES: To determine keratan sulfate (KS) concentration in the serum of foals at the early stage of growing, and to evaluate the role of serum KS as a cartilage catabolic marker, comparing its values with the fluctuation of serum alkaline phosphatase (ALP) activity as a measurement of osteoblastic activity. ANIMALS: 12 foals with normal growth and 3 foals with joint abnormalities within 18 months after birth. PROCEDURE: Measurement of KS concentration and ALP activity in serum and radiographic and physical examinations were done. RESULTS: In all foals, serum KS concentration was high from 1 week after birth to 3 months of age, while serum ALP decreased with aging. The value started to decrease rapidly from 3 to 5 months of age, then gradually reached adult values. During the first 3 months, KS concentration in male foals was significantly higher than that in female foals. In 3 foals which had joint problems, KS concentration was higher than that in normally growing foals at 1 week, and at 1, 2, and 3 months of age. CONCLUSIONS: Cartilage catabolic activity is higher in developing foals up to 3 months of age, suggesting that the immature joint at this time could be easily affected by any factor of loading. Moreover, though only 3 diseased foals were examined, higher serum KS concentration in these foals suggest that this variable might be a useful measure of joint diseases, even at an early stage of life in foals.
  • T Mori, M Okumura, M Matsuura, K Ueno, S Tokura, Y Okamoto, S Minami, T Fujinaga
    Biomaterials 18 13 947 - 51 1997年07月 [査読有り][通常論文]
     
    The effects of chitin and its derivatives on the proliferation of fibroblasts and on the production of cytokines were examined in vitro. Chitin and its derivatives showed almost no acceleratory effect on the proliferation of cultured fibroblasts. On the contrary, high-concentration 500 micrograms ml-1) D-glucosamine cultures supplemented with or without a 10% fetal calf serum (FCS) supplementation showed a significant (P < 0.05) reduction in the rate of proliferation of L929 fibroblast cells relative to control. High-concentration chitosan cultures supplemented with 10% FCS showed a significant (P < 0.05) reduction in the rate of L929 fibroblast proliferation. However, the inhibition of cell proliferation by high concentrations of chitosan did not show in cultures without FCS. Interleukin-8 (IL-8) was induced in the supernatants of rat primary cultured dermal fibroblasts stimulated with chitin and its derivatives. Chitin and its derivatives did not stimulate the production of IL-6 by mouse dermal primary cultured fibroblasts. IL-1 alpha, IL-1 beta and tumour necrosis factor-alpha were not detected in the fibroblast supernatants. These observations support the notion that cell proliferation is accelerated indirectly by chitin and its derivatives when these materials are used in vivo. In vivo findings of a angiogenesis and migration of neutrophils may be due to persistent release of IL-8 from fibroblasts.
  • T Miyamoto, M Shirahama, C Kiryu, T Mwanza, M Okumura, M Hagio, T Fujinaga
    The Journal of veterinary medical science 59 5 347 - 52 1997年05月 [査読有り][通常論文]
     
    The aim of this study was to examine renal hemodynamics at the hypovolemic and recovery phases in two different hypovolemic shock models using Doppler ultrasonography, and to compare this with systemic hemodynamics. In experiment 1, the hypovolemic phase was induced in 6 mongrel dogs by removing arterial blood at 30 ml/kg for 60 min. In the recovery phase, this blood was reinfused at 30 ml/kg over 60 min. In experiment 2, hypovolemia was induced in 12 beagle dogs by rapid blood removal until blood pressure decreased to 40 mmHg and was maintained at this pressure for 30 min. Six of the dogs were then infused with 20 ml/kg hydroxyethyl starch over 5 min, and the other 6 were infused with 60 ml/kg lactated Ringer's solution also over 5 min. Parameters for systemic and renal hemodynamics were measured by using a polygraph and the Doppler method, respectively. The decrease of diastolic blood flow, resulted in an increase of vessel resistance, and was detected in the hypovolemic kidney by the Doppler method. The rapid and large volume infusion of resuscitation fluids was effective for the recovery of both systemic circulation and renal blood flow, however this induced an increase of kidney vessel resistance, a result of the autoregulation mechanism of the kidney. The changes in these parameters at the main renal artery and interlobar artery were similar.
  • M Okumura, M Asano, T Fujinaga
    The Journal of veterinary medical science 59 2 137 - 9 1997年02月 [査読有り][通常論文]
     
    The optimum pH for the measurement of serum p-phenylenediamine oxidase (Ox) activity was given (pH 6.6), and the relationship between serum ceruloplasmin (Cp) concentration and its Ox activity was established in healthy adult horses. In adult horses, serum antigenic Cp concentrations were measured by the single radial immunodiffusion (SRID) method with the affinity-purified antibody to equine plasma Cp and compared with its Ox activity. Efficient co-relation between Cp concentration and Ox activity in the sera (r = 0.93) and its Ox/Cp ratio were given. These results might contribute to the calculation of antigenic Cp concentration from its Ox activity, the analysis of holo-Cp content in serum, and the research for copper metabolism in thoroughbred horses.
  • T Mwanza, T Miyamoto, M Okumura, M Hagio, T Fujinaga
    The Japanese journal of veterinary research 44 3 179 - 88 1996年12月 [査読有り][通常論文]
     
    The aim of this study is to provide a description of the ultrasonographic and angiographic anatomy of the hepatic and portal veins in normal dogs. Knowledge of this anatomy is essential for the proper diagnosis and correction of hepatic vessel anomalies like portal systemic shunts. Cranial mesenteric portography was done in 10 adult mongrel dogs to visualize the portal vein and its branches while the hepatic veins were visualized using the transvenous retrograde angiography method. Longitudinal and transverse ultrasound scans of the liver were done in the same dogs in left and right lateral positions in order to determine the best positions for imaging the different liver vessels. The distribution pattern of the hepatic and portal veins could be demonstrated by both ultrasonography and contrast angiography. Hepatic arteries are not usually visualized ultrasonographically. Ultrasound provided dynamic information that could not be obtained by contrast radiography. Super imposition of all vessels in the angiographic studies made the description of the branches of the liver vessels difficult. The results of the present study suggest that both methods may provide important complementary information in the diagnosis and planning of surgical procedures for the correction of vessel anomalies in the canine liver.
  • T Miyamoto, M Hagio, T Mwanza, M Okumura, T Fujinaga
    The Journal of veterinary medical science 57 6 1101 - 3 1995年12月 [査読有り][通常論文]
     
    An 8-month-old cat with bilateral hindlimb paresis was admitted. Radiography revealed a curvature of the thoracic spine and a partial deformation of thoracolumbar vertebrae (dorsal extrusion of the cranial and caudal edges). Compression of the spinal cord (T9-L1 and L6-7) was found by myelography. Medicinal treatment did not yield the desired result. Hemilaminectomy and laminectomy were done to relieve pressure on the spinal cord leading to improved gait. It is, however, not clear whether this syndrome was primarily caused by metabolic or genetic factors.
  • M Satoh, T Fujinaga, M Okumura, M Hagio
    American journal of veterinary research 56 10 1286 - 91 1995年10月 [査読有り][通常論文]
     
    To measure the concentration of serum amyloid A (sAA) protein in horses, a sensitive and highly reproducible sandwich (ELISA) was established, using affinity purified SAA antibody. Results of the ELISA were found to have a high correlation (r = 0.95) with those of the single radial immunodiffusion test. Equine SAA concentration was measured by use of this ELISA. In clinically normal horses, the concentration of SAA was high immediately after birth to 2 weeks of age. After that, SAA concentration had periodic fluctuations in the range of approximately 1.0 to 30 micrograms/ml. Mean (+/- SD)) concentrations of SAA in foals (< or = 12 months old) and adult horses (> or = 18 months old) were 21.23 +/- 12.20 and 14.93 +/- 9.07 micrograms/ml, respectively. In mares during the perinatal period, SAA concentration remained stable within the reference range before parturition. It increased quickly after delivery, and reached a peak value of 101.29 +/- 98.82 micrograms/ml on postpartum day 3, then began to decrease, at postpartum week 2, to the reference range by the end of postpartum month 1. In horses with experimentally induced inflammation, SAA concentration increased quickly and reached approximately four- to 40-fold increase over the pretreatment value on day 1 and remained high on days 2 to 6 after treatment. It then returned to the baseline value by 2 to 4 weeks in association with disappearance of local signs of inflammation. The SAA concentration was high in most horses with clinical signs of inflammation. It was concluded from these data that this ELISA is sensitive and reliable for measuring SAA in horses.
  • T Miyamoto, M Hagio, T Mwanza, T Kobayashi, M Okumura, T Fujinaga
    The Journal of veterinary medical science 57 4 785 - 8 1995年08月 [査読有り][通常論文]
     
    The aim of this study was to estimate the accuracy of the quantitative measurement of renal blood flow using the non-invasive method, Doppler ultrasonography. First, we determined a method of approach to the renal artery in order to detect the rate and pattern of flow, using 8 adult beagles under general anesthesia. Secondly, we examined the renal arterial blood flow using the Doppler method and electromagnetic flowmeter using 7 mongrel dogs. A significant correlation (r = 0.879; p < 0.01) was found between two methods, though the Doppler system tended to indicate high values than the electromagnetic flowmeter. Our study suggests that the Doppler method is a useful non-invasive technique of measuring renal blood flow in the dog.
  • Y Nunokawa, T Fujinaga, T Taira, M Okumura, K Yamashita, N Tsunoda, M Hagio
    The Journal of veterinary medical science 55 6 1011 - 6 1993年12月 [査読有り][通常論文]
     
    Serum amyloid A protein (SAA) was isolated from equine acute-phase serum by repeating Sephadex G-75 gel filtration 3 times. Quantitative measurement of equine SAA was performed by the single radial immunodiffusion technique with rabbit anti-equine SAA serum. In clinically normal horses, the SAA concentration remained relatively high from immediately after birth up to 1 week of age. After this the concentration showed periodic fluctiation in the range of approximately 13 to 30 micrograms/ml. The mean (+/- SD) concentration of SAA in foals (< or = 12 months old) and in adult horses (> or = 18 months old) was 19.37 +/- 9.41 and 21.53 +/- 9.81 micrograms/ml, respectively. In mares during the perinatal period, the SAA concentration remained stable and within the normal range for 4 months before parturition. After foaling, it increased quickly and reached a peak value of 136.78 +/- 56.74 micrograms/ml on day 3 postpartum, and then began to decrease at 2 weeks postpartum returning to within the normal range by 1 month postpartum. In horses with experimentally induced inflammation, the SAA concentration increased quickly, and reached the highest value, approximately 4 to 20 times higher than pre-treatment values, on day 2 after treatment. It then returned to the base line values within 10 days to 4 weeks, concurrent with the disappearance of local inflammatory signs. The SAA concentration was very high in most horses with clinical signs of inflammation. It was concluded from these data that equine SAA was a sensitive acute-phase reactive protein which increased in the early phase of various acute inflammations.
  • T Taira, T Fujinaga, K Tamura, M Izumi, H Itoh, N Tsunoda, K Yamashita, M Okumura, S Mizuno
    American journal of veterinary research 53 6 961 - 5 1992年06月 [査読有り][通常論文]
     
    Equine alpha 1-acid glycoprotein (alpha 1AG) was isolated from equine serum by successive ammonium precipitation, anion- and cation-exchange chromatographies, and gel filtration. Purified equine alpha 1AG had a molecular weight of 46,000 +/- 1,000, and contained 31.4% carbohydrate. Gel isoelectric focusing revealed an isoelectric point range of 2.8 to 3.7. With immunoelectrophoresis, it was found that alpha 1AG migrated to the alpha 1-globulin region. Single radial immunodiffusion was used for quantitative measurement of alpha 1AG in equine serum. In clinically normal foals, serum alpha 1AG was undetectable (less than or equal to 20 micrograms/ml) in less than or equal to 7-day-old foals, but was detected by 14 days. The alpha 1AG concentration (mean +/- SD) increased to reach mean adult values of 99.23 +/- 26.90 micrograms/ml by 1 year of age. The alpha 1AG concentration in pregnant mares decreased at 2 to 3 months before parturition, then gradually increased until 1 day after parturition, when a brief decrease was observed. The concentration increased again at 2 weeks after foaling, then a decrease was observed, after which the alpha 1AG concentration increased again by 2 to 4 months after parturition. The concentration of serum alpha 1AG quickly rose to peak values 2 to 3 days after castration and jejunojejunostomy in adult horses, returning to baseline values by 14 to 28 days after surgery. The alpha 1AG was concluded to be an acute-phase reactive protein in horses.
  • T Taira, T Fujinaga, M Okumura, K Yamashita, N Tsunoda, S Mizuno
    The Journal of veterinary medical science 54 3 435 - 42 1992年06月 [査読有り][通常論文]
     
    Haptoglobin (Hp) was isolated from equine serum by ammonium sulphate precipitation, anion-exchange chromatography and gel filtration. Equine Hp which migrated to the alpha 2-globulin region in electrophoresis, contained 2 fractions with molecular weights (NW) of 108,000 and 105,000, and each fraction consisting of 2 subunits. Quantitative measurement of Hp in equine serum was performed by the single radial immunodiffusion method using anti-equine Hp serum. In clinically normal horses, the highest concentration of serum Hp was found in newborn foals and a high value was maintained until 12 months of age. The concentration then decreased with age. Normal Hp values were 5.25 +/- 2.36 mg/ml in foals (less than or equal to 12 months old), 2.19 +/- 1.54 mg/ml in adult horses (greater than or equal to 18 months old) and 3.62 +/- 0.81 mg/ml in all horses. Serum Hp concentration in mares during the perinatal period in comparison with the normal adult female was high for 4 months pre-partum, a passing increase at delivery, and then decreased at 2 weeks post-partum returning to normal within 1 month of delivery. In horses with experimentally-induced inflammation, serum Hp concentration began to increase immediately after treatment and reached the highest value, 1.5 to 9 times higher than those of pre-treatment at 2 to 5 days, then decreased within 4 weeks. It was also elevated in most cases of horses with clinically inflammatory signs.
  • M Okumura, T Fujinaga, K Yamashita, N Tsunoda, S Mizuno
    American journal of veterinary research 52 12 1979 - 85 1991年12月 [査読有り][通常論文]
     
    Ceruloplasmin (Cp) was isolated from fresh equine plasma by precipitation, cellulose chromatography, and improved ion-exchange chromatography. Purified equine Cp is a glycoprotein having a molecular weight of approximately 115,000. In electrophoresis, equine Cp migrated to the alpha 1-globulin region, its isoelectric point was about 4.15 and consisted of about 890 amino acid residues. Serum Cp concentration was measured by use of the single radial immunodiffusion method. In clinically normal horses, the mean (+/- SD) serum Cp concentration of newborn foals was 2.87 +/- 0.40 mg/ml and that of 3-month-old foals was 5.02 +/- 0.92 mg/ml, which was similar to the adult value. It reached a peak of 6.06 +/- 0.74 mg/ml in 2-year-old horses. The Cp concentration in mares was not statistically different for the perinatal period, but it decreased immediately before and after delivery. Concentration of Cp increased at 6 days after IM administration of turpentine oil, castration, or jejunojejunostomy in adult horses, and increased to peak values twice as high as baseline values at 7 to 14 days, returning to baseline values at 28 days after treatment. We concluded that equine serum Cp is an acute-phase reactive protein increased in the intermediary or later phase of acute inflammation.
  • K Yamashita, T Fujinaga, M Okumura, M Takiguchi, N Tsunoda, S Mizuno
    The Journal of veterinary medical science 53 6 1019 - 24 1991年12月 [査読有り][通常論文]
     
    The serum concentration of C-reactive protein (CRP) in horses was measured by single radial immunodiffusion (SRID) using rabbit anti-equine CRP serum to evaluate the significance of CRP as an acute-phase reactive protein. In serum samples of clinically normal newborn thoroughbred foals before being given colostrum, serum CRP was not detectable (less than or equal to 1 microgram/ml). The serum CRP concentration was found to increase quickly and reach a peak level of approximately 14.1 micrograms/ml in 12-month-old horses. Then, it decreased gradually to reach a low value of 5.4 micrograms/ml in the 4-year-old. In horses over 5-year-old, serum CRP concentration was stable at values of 7 to 8 micrograms/ml. In mares during the peri-natal period, serum CRP concentration decreased at 2 months before delivery, showed moderate changes just before and after delivery, and then increased to relatively high level by 2 months after delivery. The concentration of serum CRP was found to be increased at 24 hours after experimentally inflammatory stimuli in adult horses, and reached peak values of 3 to 6 times as high as the base line values at 3 to 5 days. Serum CRP concentration returned to base line values by 2 to 3 weeks after treatments. It is clear from these data that CRP is one of the acute-phase reactive proteins in horses.
  • 藤永 徹, 諏訪隆彦, 成清美智代, 奥村正裕, 井上久美子, 滝口満喜, 村上正晃, 田島誉士, 水野信哉, 大友勘十郎
    日本獣医師会誌 42 491 - 494 1989年 [査読有り][通常論文]

書籍

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  • 小動物の関節疾患
    2004年
  • 小動物の関節疾患
    2004年
  • 小動物の関節疾患
    2004年
  • イヌ骨肉腫由来ヌードマウス高肺転移モデルを用いた分化誘導療法の検討
    2000年
  • 犬と猫の腫瘍疾患に対するアガリクス茸およびサメ軟骨投与の臨床学的効果
    2000年
  • 犬の根尖部apical deltaの形成と歯周疾患に対するエナメルマトリックスタンパクの歯周組織再生効果
    2000年
  • キトサンの腫瘍増殖および転移に及ぼす影響
    2000年
  • 好中球はオステオポンチン産生能を有する
    2000年
  • ネコの乳癌に対する分化誘導療法の有用性に関する基礎的研究
    2000年
  • いたみのマネージメント 症例別推奨プロトコールについて考える サプリメント サプリメントを活用した臨床報告
  • 海綿骨移植

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  • 犬の心基底部腫瘍に対する放射線治療における過喚起誘発性無呼吸と強度変調回転照射法を用いた呼吸性移動対策
    加藤 大, 出口 辰弥, 細谷 謙次, 金 尚昊, 石塚 友人, 華園 究, 高木 哲, 星野 有希, 奥村 正裕 北海道獣医師会雑誌 61 (8) 336 -336 2017年08月 [査読無し][通常論文]
  • 頸部椎間板ヘルニアに起因する呼吸障害を人工呼吸で管理したミニチュアダックスフンドの2例
    小島 一輝, 石塚 友人, 高木 哲, 越後 良介, 武内 亮, 村上 祥子, 金 尚昊, 星野 有希, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 61 (8) 337 -337 2017年08月 [査読無し][通常論文]
  • 犬の変性性腰仙椎狭窄症に対するX線とMRIを用いた画像解析に関する検討
    木原 秀哉, 越後 良介, 華園 究, 金 尚昊, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 61 (8) 338 -338 2017年08月 [査読無し][通常論文]
  • 耳下腺導管の開口部切開・拡張により治癒した唾石症の犬の1例
    竹内 恭介, 高木 哲, 細谷 謙次, 星野 有希, 金 尚昊, 華園 究, 伊丹 貴晴, 石塚 友人, 奥村 正裕 北海道獣医師会雑誌 61 (8) 344 -344 2017年08月 [査読無し][通常論文]
  • 下顎に発生した血管過誤腫の猫の1例
    村上 祥子, 高木 哲, 賀川 由美子, 石塚 友人, 華園 究, 金 尚昊, 星野 有希, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 61 (8) 344 -344 2017年08月 [査読無し][通常論文]
  • 犬の腫瘍におけるPI3K/mTOR二重阻害剤を用いたテーラーメイド治療のための効果予測因子の探索
    村瀬 優介, 細谷 謙次, 佐藤 敬近, 金 尚昊, 奥村 正裕 日本獣医学会学術集会講演要旨集 160回 457 -457 2017年08月 [査読無し][通常論文]
  • イヌの腫瘍細胞株由来Cancer stem-like cellsの放射線反応性の解析
    出口 辰弥, 細谷 謙次, 村瀬 優介, 金 尚昊, 奥村 正裕 日本獣医学会学術集会講演要旨集 160回 460 -460 2017年08月 [査読無し][通常論文]
  • コラーゲン誘発性関節炎(CIA)ラットにおけるポリ硫酸ペントサン(PPS)の抗関節炎作用(Anti-arthritis effect of Pentosan Polysulfate(PPS) in rats with collagen-induced arthritis(CIA))
    Wijekoon Suranji, Bwalya Eugene C, 青島 圭佑, Fang Jing, 金 尚昊, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 160回 468 -468 2017年08月 [査読無し][通常論文]
  • ポリ硫酸ペントサン(PPS)は犬関節軟骨細胞におけるIL-1β誘導性iNOS、c-JUNおよびHIF-1αの上方制御を抑制する(Pentosan polysulfate(PPS) inhibits IL-1β induced iNOS, c-Jun and HIF-1α upregulation in canine articular chondrocytes)
    Bwalya Eugene C, Wijekoon Suranji, 金 尚昊, Fang Jing, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 160回 469 -469 2017年08月 [査読無し][通常論文]
  • 眼窩下動脈結紮下でRadical Maxillectomyを行った犬の一例
    武内 亮, 細谷 謙次, 伊丹 貴晴, 石塚 友人, 高木 哲, 奥村 正裕 日本獣医麻酔外科学雑誌 48 (Suppl.1) 366 -366 2017年06月 [査読無し][通常論文]
  • 長期管理中にSUBが閉塞した猫の1例
    星 清貴, 高木 哲, 金 尚昊, 星野 有希, 細谷 謙次, 奥村 正裕 日本獣医麻酔外科学雑誌 48 (Suppl.1) 367 -367 2017年06月 [査読無し][通常論文]
  • 環椎・軸軸不安定症の犬におけるCTおよびMRI所見の回顧的検討
    華園 究, 大田 寛, 越後 良介, 細谷 謙次, 金 尚昊, 伊丹 貴晴, 石塚 友人, 奥村 正裕 動物臨床医学会年次大会プロシーディング 37回 (2) 293 -294 2016年11月 [査読無し][通常論文]
  • 真菌性椎間板脊椎炎の犬の一例
    金 尚昊, 細谷 謙次, 村上 祥子, 奥村 正裕 日本獣医麻酔外科学雑誌 47 (Suppl.2) 193 -193 2016年11月 [査読無し][通常論文]
  • 腫瘍随伴低血糖を呈した胃消化管間質腫瘍の犬の1例
    星野 有希, 渡辺 清正, 石塚 友人, 高木 哲, 細谷 謙次, 金 尚昊, 奥村 正裕 日本獣医麻酔外科学雑誌 47 (Suppl.2) 213 -213 2016年11月 [査読無し][通常論文]
  • リン酸トセラニブ投与を実施した再発性鼻腔腫瘍の犬14症例の回顧的研究
    出口 辰弥, 細谷 謙次, 金 尚昊, 高木 哲, 星野 有希, 奥村 正裕 北海道獣医師会雑誌 60 (8) 409 -409 2016年08月 [査読無し][通常論文]
  • 頭蓋内腫瘤性病変の犬64例における放射線治療成績と予後因子の解析
    細谷 謙次, 金 尚昊, 出口 辰弥, 星 清貴, 武内 亮, 坂本 英一, 華園 究, 石塚 友人, 柄本 浩一, 星野 有希, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 60 (8) 414 -414 2016年08月 [査読無し][通常論文]
  • スコティッシュフォールドの骨軟骨形成不全症に対して低線量放射線療法を実施した1症例
    星 清貴, 細谷 謙次, 金 尚昊, 星野 有希, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 60 (8) 415 -415 2016年08月 [査読無し][通常論文]
  • 犬の免疫介在性関節炎の疫学的および臨床的特徴
    永田 矩之, 中村 健介, 森下 啓太郎, 越後 良介, 奥村 正裕, 佐々木 東, 大田 寛, 滝口 満喜 北海道獣医師会雑誌 60 (8) 416 -416 2016年08月 [査読無し][通常論文]
  • 重度の尿管拡張・狭窄に対して側側吻合により尿路変更した猫の1症例
    武内 亮, 高木 哲, 星 清貴, 細谷 謙次, 星野 有希, 金 尚昊, 伊丹 貴晴, 石塚 友人, 奥村 正裕 北海道獣医師会雑誌 60 (8) 421 -421 2016年08月 [査読無し][通常論文]
  • 猫の全身麻酔導入時の安全な気管挿管のためのロクロニウム併用
    佐藤 敬近, 石塚 友人, 金 尚昊, 星野 有希, 細谷 謙次, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 60 (8) 423 -423 2016年08月 [査読無し][通常論文]
  • ペントサン多硫酸ナトリウムはアルギン酸ビーズ培養中の内包化単層関節軟骨細胞の再分化を促進する(Pentosan polysulfate Sodium promotes redifferentiaton of monolayer articular chondrocytes encapsulated in alginate beads culture)
    Bwalya Eugene C, 金 尚昊, Fang Jing, Wijekoon Suranji, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 159回 452 -452 2016年08月 [査読無し][通常論文]
  • 犬における膝蓋骨脱臼または前十字靱帯断裂を有する膝関節由来の滑膜細胞の鑑別能(Differentiation potential of synoviocytes derived from joints with concurrent patella luxation or cranial cruciate ligament rupture in dogs)
    Wijekoon Suranji, 豊田 一秀, 金 尚昊, Fang Jing, Bwalya Eugene C, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 159回 453 -453 2016年08月 [査読無し][通常論文]
  • 体重10kg前後の犬における末梢血造血幹細胞採取の条件検討
    小林 鮎実, 金 尚昊, 細谷 謙次, 加藤 大, 奥村 正裕 日本獣医学会学術集会講演要旨集 159回 453 -453 2016年08月 [査読無し][通常論文]
  • Close Up! 犬の変形性関節症治療における非ステロイド性抗炎症薬(NSAIDs)と病態修飾薬(DMOADs)使用の考え方 オンシオールとアデクァンの使用例
    奥村 正裕 CAP: Companion Animal Practice 31 (7) 73 -80 2016年07月 [査読無し][通常論文]
  • アルファキサロンとプロポフォール麻酔導入が犬の循環動態に及ぼす影響の比較
    伊丹 貴晴, 細谷 謙次, 高木 哲, 金 尚昊, 出口 辰弥, 華園 究, 奥村 正裕 日本獣医麻酔外科学雑誌 47 (Suppl.1) 279 -279 2016年06月 [査読無し][通常論文]
  • 広範囲の頭蓋骨切除後にチタンメッシュで再建した骨の多分葉状腫瘍の犬の一例
    武内 亮, 細谷 謙次, 伊丹 貴晴, 石塚 友人, 高木 哲, 奥村 正裕 日本獣医麻酔外科学雑誌 47 (Suppl.1) 341 -341 2016年06月 [査読無し][通常論文]
  • フレンチブルドッグにおける全耳道切除後の合併症について
    星野 有希, 高木 哲, 細谷 謙次, 金 尚昊, 奥村 正裕 日本獣医麻酔外科学雑誌 47 (Suppl.1) 342 -342 2016年06月 [査読無し][通常論文]
  • 十二指腸浸潤を伴うガストリノーマに対して、十二指腸-膵臓分節切除を実施した犬の1例
    星 清貴, 細谷 謙次, 金 尚昊, 星野 有希, 高木 哲, 奥村 正裕 日本獣医麻酔外科学雑誌 47 (Suppl.1) 343 -343 2016年06月 [査読無し][通常論文]
  • ソマトトロピン産生性下垂体腫瘍によりインスリン抵抗性を呈した糖尿病の猫の1例
    和泉 雄介, 細谷 謙治, 早川 小百合, 森下 啓太郎, 高木 哲, 奥村 正裕 日本獣医師会雑誌 69 (5) 293 -293 2016年05月 [査読無し][通常論文]
  • 伊丹 貴晴, 高木 哲, 武内 亮, 星 清貴, 華園 究, 中村 健介, 奥村 正裕 日本獣医麻酔外科学雑誌 47 (1) 13 -19 2016年04月 [査読無し][通常論文]
  • 犬・猫の関節炎 関節のなかで起きていること=炎症に対する治療
    奥村 正裕 CAP: Companion Animal Practice 30 (12) 81 -87 2015年12月 [査読無し][通常論文]
  • 変性性関節疾患と炎症性関節疾患
    奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.2) 35 -36 2015年12月 [査読無し][通常論文]
  • 多発性の頸部椎間板疾患に対して複数箇所のベントラルスロット形成術を行った犬17頭についての回顧的検討
    武内 亮, 越後 良介, 華園 究, 伊丹 貴晴, 石塚 友人, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.2) 220 -220 2015年12月 [査読無し][通常論文]
  • 再発性悪性末梢神経腫瘍に対して放射線治療を実施した犬の5例
    出口 辰弥, 細谷 謙次, 高木 哲, 越後 良介, 金 尚昊, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.2) 221 -221 2015年12月 [査読無し][通常論文]
  • 猫の左室流出路動的狭窄に観血的動脈血圧測定を用いて全身麻酔管理を行った1例
    伊丹 貴晴, 高木 哲, 中村 健介, 華園 究, 武内 亮, 星 清貴, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.2) 225 -225 2015年12月 [査読無し][通常論文]
  • 猫の尿管結石・閉塞23例における内科療法の反応と外科手術法の検討
    星 清貴, 高木 哲, 細谷 謙次, 星野 有希, 中村 健介, 森下 啓太郎, 金 尚昊, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.2) 227 -227 2015年12月 [査読無し][通常論文]
  • 肝臓未分化肉腫に併発した肝嚢胞によりBudd-Chiari様症候群を呈した犬の一例
    下出 亜里咲, 高木 哲, 伊丹 貴晴, 金 尚昊, 星野 有希, 細谷 謙次, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.2) 235 -235 2015年12月 [査読無し][通常論文]
  • 脊髄への播種が疑われた神経膠腫の犬の2例
    星 清貴, 細谷 謙次, 武内 亮, 金 尚昊, 星野 有希, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 59 (8) 386 -386 2015年08月 [査読無し][通常論文]
  • ソマトトロピン産生性下垂体腫瘍によりインスリン抵抗性を呈した糖尿病の猫の1例
    和泉 雄介, 細谷 謙次, 早川 小百合, 森下 啓太郎, 華園 究, 星野 有希, 高木 哲, 金 尚昊, 奥村 正裕 北海道獣医師会雑誌 59 (8) 394 -394 2015年08月 [査読無し][通常論文]
  • 異所性副腎皮質腺癌により多飲多尿を認めた犬の1例
    出口 辰弥, 細谷 謙次, 松本 隆, 賀川 由美子, 高木 哲, 星野 有希, 金 尚昊, 奥村 正裕 北海道獣医師会雑誌 59 (8) 395 -395 2015年08月 [査読無し][通常論文]
  • 尺骨滑車切痕骨折の癒合不全に対して上腕三頭筋腱移植を行った犬1症例
    武内 亮, 越後 良介, 下出 亜里咲, 高木 哲, 細谷 謙次, 星野 有希, 金 昊尚, 奥村 正裕 北海道獣医師会雑誌 59 (8) 399 -399 2015年08月 [査読無し][通常論文]
  • 遠位橈尺骨骨折に対してテーラーメイドプレートを用いて整復を行った犬1症例
    豊田 一秀, 金 尚昊, 細谷 謙次, 安田 準, 首藤 文栄, 奥村 正裕 北海道獣医師会雑誌 59 (8) 400 -400 2015年08月 [査読無し][通常論文]
  • 胸骨切開下ベントラルスロット術にて治療した頸胸椎移行部における椎間板ヘルニアの犬の3例
    大脇 稜, 越後 良介, 星 清貴, 和泉 雄介, 高木 哲, 星野 有希, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 59 (8) 400 -400 2015年08月 [査読無し][通常論文]
  • 内視鏡併用下にて尿管側より腎結石摘出術を行った犬の1例
    下出 亜里咲, 高木 哲, 金 尚昊, 星野 有希, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 59 (8) 401 -401 2015年08月 [査読無し][通常論文]
  • Hydropulsion法により気道閉塞解除を実施した犬の1例
    坂本 英一, 細谷 謙次, 高木 哲, 星野 有希, 金 尚昊, 奥村 正裕 北海道獣医師会雑誌 59 (8) 403 -403 2015年08月 [査読無し][通常論文]
  • 症例から学ぶシリーズ 猫の下顎扁平上皮癌において下顎4分の3切除を行った7例
    岩木 芳美, 細谷 謙次, 金 尚昊, 星野 有希, 高木 哲, 奥村 正裕 Joncol: Japanese Journal of Veterinary Clinical Oncology 11 (2) 81 -84 2015年07月 [査読無し][通常論文]
  • 胸水貯留を認めた胸腔内異所性肝の犬の1例
    岩木 芳美, 高木 哲, 森下 啓太郎, 高瀬 奈美, 華園 究, 細谷 謙次, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.1) 228 -228 2015年06月 [査読無し][通常論文]
  • 鼻腔内に発生した歯原性嚢胞に対して外科治療を実施した短頭種犬の2症例
    武内 亮, 高木 哲, 大田 寛, 中村 健介, 伊丹 貴晴, 華園 究, 細谷 謙次, 賀川 由美子, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.1) 242 -242 2015年06月 [査読無し][通常論文]
  • 再発性多小葉性骨軟骨肉腫に対して定位放射線治療を行った犬の一例
    金 尚昊, 細谷 謙次, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.1) 255 -255 2015年06月 [査読無し][通常論文]
  • 胸骨正中切開アプローチによるVentral Slot術を実施した第1-2胸椎椎間板ヘルニアの犬の1例
    越後 良介, 和泉 雄介, 伊丹 貴晴, 華園 究, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.1) 257 -257 2015年06月 [査読無し][通常論文]
  • 合併症予防を目的とした腸瘻チューブの新たな固定法の検討
    星 清貴, 高木 哲, 星野 有希, 細谷 謙次, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.1) 268 -268 2015年06月 [査読無し][通常論文]
  • 犬の椎間板ヘルニアに対する経皮的レーザー椎間板減圧術(PLDD)の再発予防効果
    重富 加奈子, 小川 純也, 高木 哲, 奥村 正裕 日本獣医麻酔外科学雑誌 46 (Suppl.1) 309 -309 2015年06月 [査読無し][通常論文]
  • 多分割照射法による根治的放射線治療に関する有効性と有害事象発生率の回顧的調査
    武内 亮, 細谷 謙次, 金 尚昊, 星野 有希, 高木 哲, 奥村 正裕 日本獣医師会雑誌 68 (5) 325 -326 2015年05月 [査読無し][通常論文]
  • 慢性痛リーフレットの紹介
    左近允 巌, 浅川 誠, 今井 彩子, 枝村 一弥, 奥村 正裕, 佐野 忠士, 永延 清和, 西村 亮平, 山下 和人, 高島 一昭, 山根 義久 動物臨床医学会年次大会プロシーディング 35回 (4) 7 -10 2014年11月 [査読無し][通常論文]
  • 痛みの管理だけではない関節疾患の治療 ポリ硫酸ペントサンの可能性
    奥村 正裕 動物臨床医学会年次大会プロシーディング 35回 (3) 161 -163 2014年11月 [査読無し][通常論文]
  • 足根関節内側側副靱帯損傷に対して関節安定化術を行った犬の1例
    多田 健一郎, 白沢 信彦, 星野 有希, 高木 哲, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 58 (8) 359 -359 2014年08月 [査読無し][通常論文]
  • 腱鞘の悪性巨細胞腫の猫の1例
    和泉 雄介, 細谷 謙次, 賀川 由美子, 高木 哲, 星野 有希, 金 尚昊, 奥村 正裕 北海道獣医師会雑誌 58 (8) 360 -360 2014年08月 [査読無し][通常論文]
  • 多中心型リンパ腫の犬26例における高用量サイクロフォスファミドを用いた地固め療法の検討
    金 尚昊, 細谷 謙次, 出口 辰弥, 高木 哲, 星野 有希, 奥村 正裕 北海道獣医師会雑誌 58 (8) 361 -361 2014年08月 [査読無し][通常論文]
  • 小型犬におけるドキソルビシンの最大耐容量に関する検討
    金 尚昊, 細谷 謙次, 高木 哲, 星野 有希, 奥村 正裕 北海道獣医師会雑誌 58 (8) 362 -362 2014年08月 [査読無し][通常論文]
  • 犬における全腹部照射(Whole Abdominal Irradiation:WAI)プロトコールの検討
    出口 辰弥, 細谷 謙次, 高木 哲, 星野 有希, 中村 健介, 金 尚昊, 奥村 正裕 北海道獣医師会雑誌 58 (8) 362 -362 2014年08月 [査読無し][通常論文]
  • 多分割照射法による根治的放射線治療に関する有害事象発生率と有効性の回顧的調査
    武内 亮, 細谷 謙次, 星野 有希, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 58 (8) 363 -363 2014年08月 [査読無し][通常論文]
  • 胸骨肋軟骨部分切除および横隔膜前進術にて治療した漏斗胸の猫の1例
    三谷 浩気, 高木 哲, 武内 亮, 北村 二郎, 星野 有希, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 58 (8) 371 -371 2014年08月 [査読無し][通常論文]
  • 猫の下顎扁平上皮癌において下顎4分の3切除を行った6症例
    岩木 芳美, 細谷 謙次, 高木 哲, 星野 有希, 金 尚昊, 奥村 正裕 北海道獣医師会雑誌 58 (8) 373 -373 2014年08月 [査読無し][通常論文]
  • 4歳齢の毛球の食道内塞栓により発見された右大動脈弓遺残の猫の1例
    村上 翔輝, 高木 哲, 中村 健介, 岩木 芳美, 金 尚昊, 星野 有希, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 58 (8) 373 -373 2014年08月 [査読無し][通常論文]
  • 副腎摘出術を実施した犬19例の手術成績に関する回顧的検討
    星 清貴, 高木 哲, 細谷 謙次, 金 尚昊, 星野 有希, 奥村 正裕 北海道獣医師会雑誌 58 (8) 379 -379 2014年08月 [査読無し][通常論文]
  • 実験的軟骨欠損における犬の関節軟骨下骨病変の特徴
    金 尚昊, 小谷 章朗, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 157回 495 -495 2014年08月 [査読無し][通常論文]
  • 山崎 裕毅, 山口 朋生, 細谷 謙次, 奥村 正裕 日本獣医師会雑誌 67 (7) 518 -521 2014年07月 [査読無し][通常論文]
  • 犬の組織球性肉腫におけるsurvivinの機能解析と治療開発への基礎的研究
    山崎 裕毅, 高木 哲, 細谷 謙次, 奥村 正裕 獣医畜産新報 67 (6) 443 -444 2014年06月 [査読無し][通常論文]
  • 肘関節形成不全と内側コンパートメント症候群に対する治療 病態生理および内科的治療
    奥村 正裕 獣医麻酔外科学雑誌 45 (Suppl.1) 115 -116 2014年06月 [査読無し][通常論文]
  • 犬組織球性肉腫における遺伝子診断法と分子標的療法の開発に関する基礎的及び臨床学的研究
    山崎 裕毅, 細谷 謙次, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 45 (Suppl.1) 143 -144 2014年06月 [査読無し][通常論文]
  • 山崎 裕毅, 高木 哲, 細谷 謙次, 星野 有希, 奥村 正裕 日本獣医師会雑誌 67 (4) 263 -268 2014年04月 [査読無し][通常論文]
  • 【診断シリーズVol.13-疾患からみた検査の考え方-関節疾患】 関節液検査
    奥村 正裕 SA Medicine 16 (1) 70 -78 2014年02月 [査読無し][通常論文]
  • 【診断シリーズVol.13-疾患からみた検査の考え方-関節疾患】 滑膜生検
    奥村 正裕 SA Medicine 16 (1) 78 -79 2014年02月 [査読無し][通常論文]
  • 閉鎖吸引ドレナージと経腸栄養により治療した壊死性膵炎の犬の一例
    高木 哲, 大田 寛, 足立 真実, 細谷 謙次, 奥村 正裕 獣医麻酔外科学雑誌 44 (Suppl.2) 236 -236 2013年12月 [査読無し][通常論文]
  • 口角動脈-上唇動脈吻合を利用した粘膜弁により口腔内組織欠損を被覆した犬の2例
    細谷 謙次, 金 昊尚, 出口 辰弥, 星野 有希, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 44 (Suppl.2) 242 -242 2013年12月 [査読無し][通常論文]
  • 関節疾患を治療するための基礎知識 ここが解れば、治療法がわかる
    奥村 正裕 動物臨床医学会年次大会プロシーディング 34回 (1) 1 -4 2013年11月 [査読無し][通常論文]
  • 犬と猫の変形性関節症OA/DJDのアップ・トゥ・デード
    奥村 正裕 動物臨床医学会年次大会プロシーディング 34回 (3) 202 -203 2013年11月 [査読無し][通常論文]
  • 犬の前十字靱帯断裂発症時における関節変形のX線学的評価
    小谷 章朗, 星野 有希, 高木 哲, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 57 (8) 384 -384 2013年08月 [査読無し][通常論文]
  • 胸部後大静脈内浸潤を伴う褐色細胞腫の犬の1症例
    足立 真実, 高木 哲, 中村 健介, 大菅 辰幸, 星野 有希, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 57 (8) 393 -393 2013年08月 [査読無し][通常論文]
  • 頸部腫瘍に対して緩和的放射線治療を実施した犬の4例
    金 尚昊, 細谷 謙次, 橋場 香野, 高木 哲, 星野 有希, 奥村 正裕 北海道獣医師会雑誌 57 (8) 394 -394 2013年08月 [査読無し][通常論文]
  • 胆嚢十二指腸吻合および胆嚢肝管吻合を行った胆管閉塞の猫の1症例
    姉川 啓文, 高木 哲, 橋場 香野, 細谷 謙次, 星野 有希, 滝口 満喜, 奥村 正裕 北海道獣医師会雑誌 57 (8) 400 -400 2013年08月 [査読無し][通常論文]
  • 犬の腫瘍血管内皮細胞の分離と性状解析
    和泉 雄介, 星野 有希, 細谷 謙次, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 156回 343 -343 2013年08月 [査読無し][通常論文]
  • 浸潤性脂肪腫に対して外科的処置を行った犬の3例
    金 尚昊, 細谷 謙次, 高木 哲, 星野 有希, 奥村 正裕 獣医麻酔外科学雑誌 44 (Suppl.1) 209 -209 2013年06月 [査読無し][通常論文]
  • 尿道ステント設置後早期に再閉塞を生じた前立腺癌の犬の1例
    細谷 謙次, 金 尚昊, 星野 有希, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 44 (Suppl.1) 272 -272 2013年06月 [査読無し][通常論文]
  • 小動物の関節炎治療 関節リウマチ治療に向けて
    奥村 正裕 獣医麻酔外科学雑誌 44 (Suppl.1) 275 -275 2013年06月 [査読無し][通常論文]
  • S. Takagi, H. Yamazaki, K. Hosoya, Y. Hoshino, M. Okumura JOURNAL OF VETERINARY INTERNAL MEDICINE 27 (3) 682 -682 2013年05月 [査読無し][通常論文]
  • H. Yamazaki, S. Takagi, Y. Hoshino, K. Hosoya, M. Okumura JOURNAL OF VETERINARY INTERNAL MEDICINE 27 (3) 682 -682 2013年05月 [査読無し][通常論文]
  • 炎症刺激による培養馬軟骨細胞の形質転換の特徴に関する研究
    金 尚昊, 海道 磨里, 細谷 謙次, 星野 有希, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 155回 186 -186 2013年03月 [査読無し][通常論文]
  • 非ステロイド性抗炎症薬に対する犬骨髄由来間葉系幹細胞の骨分化維持能
    呉 南佶, 金 尚昊, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 155回 262 -262 2013年03月 [査読無し][通常論文]
  • 犬組織球性肉腫の細胞内鉄量および鉄反応性薬剤ジヒドロアルテミシニンの細胞傷害効果の検討
    永田 理紗, 細谷 謙次, 星野 有希, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 155回 262 -262 2013年03月 [査読無し][通常論文]
  • 犬膀胱移行上皮癌におけるがん遺伝子DEKの治療標的としての有用性の検討
    岩野 公美, 山崎 裕毅, 呉 南佶, 足立 真美, 金 尚昊, 星野 有希, 高木 哲, 細谷 謙治, 奥村 正裕 日本獣医学会学術集会講演要旨集 155回 262 -262 2013年03月 [査読無し][通常論文]
  • 須永 隆文, 高木 哲, 小川 修治, 細谷 謙次, 奥村 正裕 日本獣医師会雑誌 66 (1) 57 -60 2013年01月 [査読無し][通常論文]
  • 化学療法後に急性腫瘍溶解症候群が認められた急性リンパ芽球性白血病の犬の1例
    足立 真実, 細谷 謙次, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 56 (12) 674 -677 2012年12月 [査読無し][通常論文]
     
    急性腫瘍溶解症候群(Acute tumor lysis syndrome:ATLS)は、抗腫瘍療法により急速に腫瘍細胞が溶解することによって高窒素血症や電解質異常などをきたす症候群である。進行は非常に急性であり、致死的な経過を辿ることが多いとされている。しかしながら、犬における本病態の報告例は少なく、また治療効果や予後に関する情報が不足している。今回、急性リンパ芽球性白血病と診断された症例にL-アスパラギナーゼによる化学療法を行ったところ、ATLSを発症した。診断後直ちに輸液療法と合わせてメトクロプラミドの持続点滴およびフロセミドによる利尿を行い、症例は回復した。化学療法高感受性腫瘍症例の治療においては、ATLSの高リスク症例を予測すると共に、ATLSを疑う症状を認めた場合には、診断および治療を迅速に行うことが重要であると考えられた。(著者抄録)
  • 肩甲骨全摘出術を実施した骨肉腫の大型犬の1例
    細谷 謙次, 橋場 香野, 星野 有希, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 43 (Suppl.2) 177 -177 2012年12月 [査読無し][通常論文]
  • コバルト合金(コバリオン)製犬用カスタムフィット骨プレートの試作とその適用
    奥村 正裕, 高木 哲, 細谷 謙次, 呉 南佶, 山崎 裕毅, 首藤 文榮, 御領 政信, 渡部 佑悟, 安田 準, 千葉 晶彦 動物臨床医学会年次大会プロシーディング 33回 (3) 20 -21 2012年11月 [査読無し][通常論文]
  • 慢性痛 前十字靱帯断裂 犬の慢性痛評価基準の作成(最終報告)
    左近允 巌, 浅川 誠, 今井 彩子, 枝村 一弥, 奥村 正裕, 佐野 忠士, 永延 清和, 西村 亮平, 山下 和人, 高島 一昭, 山根 義久 動物臨床医学会年次大会プロシーディング 33回 (3) 177 -181 2012年11月 [査読無し][通常論文]
  • 犬と猫の変形性関節症OA/DJDについて
    奥村 正裕 動物臨床医学会年次大会プロシーディング 33回 (3) 243 -244 2012年11月 [査読無し][通常論文]
  • 経皮的尿管ステント設置術を実施した犬の5症例
    金 尚昊, 細谷 謙次, 睦良田 尚充, 星野 有希, 高木 哲, 奥村 正裕 動物臨床医学会年次大会プロシーディング 33回 (2) 15 -16 2012年11月 [査読無し][通常論文]
  • 前大静脈症候群を主症状とした肋骨軟骨肉腫の犬の1例
    細谷 謙次, 山口 朋生, 玉井 聡, 田村 洋次郎, 高木 哲, 奥村 正裕 動物臨床医学会年次大会プロシーディング 33回 (2) 377 -378 2012年11月 [査読無し][通常論文]
  • 肩甲骨全摘出術を実施した骨肉腫の大型犬の1例
    橋場 香野, 細谷 謙次, 柘植 勇祐, 松尾 憙道, 星野 有希, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 56 (8) 404 -404 2012年08月 [査読無し][通常論文]
  • 腫瘍性疾患により前肢跛行を呈した犬の回顧的研究
    金 尚昊, 星野 有希, 細谷 謙次, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 56 (8) 405 -405 2012年08月 [査読無し][通常論文]
  • 上皮小体切除を実施した犬11症例
    星野 有希, 高木 哲, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 56 (8) 411 -411 2012年08月 [査読無し][通常論文]
  • 披裂軟骨背側に形成された炎症性腫瘤病変を喉頭切開により摘出した猫の1例
    岩野 公美, 高木 哲, 星野 有希, 細谷 謙次, 滝口 満喜, 奥村 正裕 北海道獣医師会雑誌 56 (8) 411 -411 2012年08月 [査読無し][通常論文]
  • 外科手術に関連して発生した犬の急性膵炎に関する回顧的検討
    足立 真実, 高木 哲, 星野 有希, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 56 (8) 412 -412 2012年08月 [査読無し][通常論文]
  • 包皮および陰茎の低形成を示したXX雄の犬の1例
    海道 磨里, 細谷 謙次, 永野 昌志, 棚田 敦司, 星野 有希, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 56 (8) 418 -418 2012年08月 [査読無し][通常論文]
  • 手根関節部分固定を行った第二手根骨骨折の猫一症例
    永田 理紗, 柘植 勇祐, 玉井 聡, 細谷 謙次, 星野 有希, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 56 (8) 424 -424 2012年08月 [査読無し][通常論文]
  • 唾液腺造影検査が有用であった唾液腺関連疾患の犬5例
    山崎 裕毅, 細谷 謙次, 柘植 勇祐, 星野 有希, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 56 (8) 426 -426 2012年08月 [査読無し][通常論文]
  • 小動物骨折治療のためのカスタムフィットプレート供給システムの構築
    土井 章男, 高橋 弘毅, 首藤 文榮, 片山 泰章, 長嶋 宏之, 田村 昌人, 奥村 正裕 日本獣医学会学術集会講演要旨集 154回 308 -308 2012年08月 [査読無し][通常論文]
  • コバルト合金製カスタムフィットインプラントの犬への埋込試験
    奥村 正裕, 高木 哲, 山崎 裕毅, 首藤 文榮, 御領 政信, 渡部 佑悟, 安田 準, 千葉 晶彦 日本獣医学会学術集会講演要旨集 154回 308 -308 2012年08月 [査読無し][通常論文]
  • 分子標的治療を目指した犬膀胱移行上皮癌細胞株の樹立
    星野 有希, 森 崇, 西谷 由莉, 酒井 洋樹, 奥村 正裕, 丸尾 幸嗣 日本獣医学会学術集会講演要旨集 154回 312 -312 2012年08月 [査読無し][通常論文]
  • Survivin遺伝子発現制御による犬組織球性肉腫細胞の形態と機能の変動評価
    山崎 裕毅, 高木 哲, 星野 有希, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 154回 313 -313 2012年08月 [査読無し][通常論文]
  • 犬の骨髄由来間葉系幹細胞の骨分化における非ステロイド性抗炎症薬の影響
    呉 南佶, 山崎 裕毅, 金 尚昊, 細谷 謙次, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 154回 319 -319 2012年08月 [査読無し][通常論文]
  • 山崎 裕毅, 高木 哲, 小儀 直子, 須永 隆文, 青木 由徳, 細谷 謙次, 奥村 正裕 日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association 65 (7) 530 -533 2012年07月20日 [査読無し][通常論文]
  • 奥村 正裕 ペット栄養学会誌 15 (Suppl.) 15 -16 2012年07月 [査読無し][通常論文]
  • 奥村 正裕 ペット栄養学会誌 15 (Suppl.) 27 -28 2012年07月 [査読無し][通常論文]
  • 小動物の関節炎
    奥村 正裕 獣医麻酔外科学雑誌 43 (Suppl.1) 65 -65 2012年06月 [査読無し][通常論文]
  • 背側腹壁筋群の腹側転位により人工材料を用いずに腹壁を再建した糖尿病罹患犬の1例
    細谷 謙次, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 43 (Suppl.1) 277 -277 2012年06月 [査読無し][通常論文]
  • 山崎 裕毅, 高木 哲, 須永 隆文, 細谷 謙次, 奥村 正裕 日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association 65 (5) 2012年05月20日 [査読無し][通常論文]
  • 細谷 謙次, 高木 哲, 小儀 直子, 睦良田 尚充, 奥村 正裕 日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association 65 (5) 390 -390 2012年05月20日 [査読無し][通常論文]
  • 奥村 正裕 Clinic note : journal of clinical daily treatment for small animals 8 (4) 61 -69 2012年04月 [査読無し][通常論文]
  • 奥村 正裕, Lascelles B. Duncan X, 西村 亮平 Clinic note : journal of clinical daily treatment for small animals 8 (4) 104 -110 2012年04月 [査読無し][通常論文]
  • 培養馬腱細胞における分化マーカーおよび細胞代謝関連因子の解析
    野村 基惟, 金 尚昊, 細谷 謙次, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 153回 193 -193 2012年03月 [査読無し][通常論文]
  • 犬肥満細胞腫由来抗腫瘍薬耐性細胞の性状と耐性関連因子の検討
    富永 なつほ, 高木 哲, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 153回 266 -266 2012年03月 [査読無し][通常論文]
  • 小型犬における変形性関節症の治療と管理
    奥村 正裕, 原田 恭治, Lascelles B. Duncan X J-VET 24 (12) 42 -48 2011年12月 [査読無し][通常論文]
  • 口唇原発悪性腫瘍の犬4例における切除範囲および再建法の検討
    細谷 謙次, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 42 (Suppl.2) 195 -195 2011年12月 [査読無し][通常論文]
  • 犬の骨折治療時の非ステロイド性抗炎症薬投与骨折治癒とシクロオキシゲナーゼ阻害について
    奥村 正裕 獣医麻酔外科学雑誌 42 (Suppl.2) 237 -238 2011年12月 [査読無し][通常論文]
  • 奥村 正裕, 原田 恭治, Lascelles B. Duncan X J-vet : EBM & informed consent 24 (12) 42 -48 2011年12月 [査読無し][通常論文]
  • 【見落としはない?犬と猫の変形性関節症!】 総論 犬と猫における変形性関節症の管理 現在獣医師が使用できる消炎鎮痛剤 それぞれの特性と使いどころ
    奥村 正裕 MVM 20 (7) 6 -13 2011年11月 [査読無し][通常論文]
  • どこまで薬でどこから手術?あなたにもわかる関節の病気!
    奥村 正裕 動物臨床医学会年次大会プロシーディング 32回 (1) 38 -42 2011年11月 [査読無し][通常論文]
  • 膀胱タンポナーデの猫の2症例
    青木 由徳, 高木 哲, 山崎 真大, 細谷 謙次, 奥村 正裕 動物臨床医学会年次大会プロシーディング 32回 (2) 209 -210 2011年11月 [査読無し][通常論文]
  • 巨大食道症に続発した胃食道重積の犬の1例
    富永 なつほ, 高木 哲, 細谷 謙次, 本田 英隆, 奥村 正裕 北海道獣医師会雑誌 55 (8) 389 -389 2011年08月 [査読無し][通常論文]
  • 表面抗原解析による補助的診断が有用であった肺組織球肉腫の犬3例
    山崎 裕毅, 高木 哲, 睦良田 尚充, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 55 (8) 392 -392 2011年08月 [査読無し][通常論文]
  • 後頭部に発生した髄膜腫を摘出手術した猫の1症例
    小坂井 崇, 高木 哲, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 55 (8) 393 -393 2011年08月 [査読無し][通常論文]
  • イヌの悪性腫瘍による上部尿路閉塞に対する尿管ステント術
    睦良田 尚充, 細谷 謙次, 足立 真実, 山崎 裕毅, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 55 (8) 394 -394 2011年08月 [査読無し][通常論文]
  • 椎孔内に浸潤した悪性末梢神経鞘腫の犬の5例
    細谷 謙次, 高木 哲, 小儀 直子, 睦良田 尚充, 奥村 正裕 北海道獣医師会雑誌 55 (8) 394 -394 2011年08月 [査読無し][通常論文]
  • 後肢麻痺を呈した多発性骨髄腫の犬の2症例
    足立 真実, 高木 哲, 細谷 謙次, 野村 基惟, 本田 英隆, 奥村 正裕 北海道獣医師会雑誌 55 (8) 397 -397 2011年08月 [査読無し][通常論文]
  • 重度の神経学的異常を伴う腰椎椎体骨折に対して外科的処置を行った犬の2症例
    野村 基惟, 青木 由徳, 細谷 謙次, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 55 (8) 401 -401 2011年08月 [査読無し][通常論文]
  • 柴犬の膝蓋骨内方脱臼における後肢骨格変形の画像的特徴
    青木 由徳, 須永 隆文, 山崎 裕毅, 細谷 謙次, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 55 (8) 401 -401 2011年08月 [査読無し][通常論文]
  • 膝蓋高位症に関連した両側性膝蓋骨内方脱臼を呈した犬1症例
    梅田 亮, 青木 由徳, 細谷 謙次, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 55 (8) 402 -402 2011年08月 [査読無し][通常論文]
  • 犬関節軟骨細胞における外因性ヒアルロン酸のヒアルロン酸合成酵素遺伝子発現に対する効果
    須永 隆文, 呉 南佶, 山崎 裕毅, 足立 真実, 細谷 謙次, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 152回 294 -294 2011年08月 [査読無し][通常論文]
  • 犬の組織球肉腫におけるSurvivin遺伝子発現の予後因子としての評価
    山崎 裕毅, 高木 哲, 呉 南佶, 須永 隆文, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 152回 302 -302 2011年08月 [査読無し][通常論文]
  • 歩行不可能な四肢不全麻痺を呈する環椎軸椎亜脱臼罹患犬2例に対する腹側椎体固定術の治療効果
    細谷 謙次, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 42 (Suppl.1) 272 -272 2011年06月 [査読無し][通常論文]
  • 犬の骨芽細胞分化および成熟における非ステロイド性抗炎症薬の影響
    呉 南佶, 須永 隆文, 山崎 裕毅, 細谷 謙次, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 151回 186 -186 2011年03月 [査読無し][通常論文]
  • 馬の腱および軟骨の細胞外基質代謝関連分子に関する基礎的研究
    金 尚昊, 高木 哲, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 151回 186 -186 2011年03月 [査読無し][通常論文]
  • 犬由来培養移行上皮癌細胞に対するラパマイシンの細胞障害性効果およびその作用機序に関する考察
    中村 仁美, 細谷 謙次, 高木 哲, 山崎 裕毅, 奥村 正裕 日本獣医学会学術集会講演要旨集 151回 270 -270 2011年03月 [査読無し][通常論文]
  • 【治療シリーズ 私はこうしている 神経・筋肉・関節疾患】 感染性関節炎
    奥村 正裕 SA Medicine 12 (6) 55 -56 2010年12月 [査読無し][通常論文]
  • 【治療シリーズ 私はこうしている 神経・筋肉・関節疾患】 股関節形成異常
    奥村 正裕 SA Medicine 12 (6) 56 -57 2010年12月 [査読無し][通常論文]
  • 関節液の採取と評価
    奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.2) 61 -62 2010年12月 [査読無し][通常論文]
  • 外科的切除を行った前大静脈内浸潤の認められた胸腺腫の犬の一例
    高木 哲, 足立 真美, 渡邉 武史, 細谷 謙次, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.2) 187 -187 2010年12月 [査読無し][通常論文]
  • MRSA感染による橈尺骨癒合不全の犬の一症例 感染部切除と抗菌剤含有ゼラチン製剤による治療経験
    青木 由徳, 小儀 直子, 金 尚昊, 高木 哲, 細谷 謙次, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.2) 192 -192 2010年12月 [査読無し][通常論文]
  • 多発性非細菌性化膿性肉芽腫性骨髄炎を認めたミニチュアダックスフンドの1症例
    大崎 智弘, 細谷 謙次, 青木 由徳, 落合 謙爾, 梅村 孝司, 高木 哲, 南 三郎, 岡本 芳晴, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.2) 197 -197 2010年12月 [査読無し][通常論文]
  • 尿管ステントおよび尿道バルーン拡張術により管理した前立腺癌の犬の一例
    細谷 謙次, 高木 哲, 末廣 昌, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.2) 199 -199 2010年12月 [査読無し][通常論文]
  • 飼い主さんと動物に感謝される関節炎治療法!関節内注射の実用法 肩関節から仙腸関節まで
    奥村 正裕 動物臨床医学会年次大会プロシーディング 31回 (1) 63 -64 2010年11月 [査読無し][通常論文]
  • ガストリン産生性カルチノイドに対し徐放性オクトレオチド製剤を使用した犬の1例
    細谷 謙次, 金子 一成, 滝口 満善, 中村 健介, 高木 哲, 奥村 正裕 動物臨床医学会年次大会プロシーディング 31回 (2) 193 -194 2010年11月 [査読無し][通常論文]
  • 犬の関節疾患Update 跛行と疼痛の改善に向けて
    奥村 正裕 動物臨床医学会年次大会プロシーディング 31回 (3) 183 -185 2010年11月 [査読無し][通常論文]
  • 犬の組織球系疾患におけるアポトーシス抑制因子の発現
    山崎 裕毅, 高木 哲, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 150回 298 -298 2010年09月 [査読無し][通常論文]
  • 犬培養滑膜細胞に対するテポキサリンのアポトーシス誘導作用と細胞内シグナル伝達系への関与
    須永 隆文, 細谷 謙次, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 150回 304 -304 2010年09月 [査読無し][通常論文]
  • 胸腰部椎間板ヘルニア様の臨床症状を呈するもCT検査で異常を認めなかった犬の10例
    足立 真実, 高木 哲, 細谷 謙次, 山根 由久, 奥村 正裕 北海道獣医師会雑誌 54 (8) 446 -446 2010年08月 [査読無し][通常論文]
  • 頭蓋骨より発生した多小葉性骨軟骨肉腫の1症例
    小坂井 崇, 細谷 謙次, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 54 (8) 449 -449 2010年08月 [査読無し][通常論文]
  • 舌の摘出手術を行った扁平上皮癌の犬の1症例
    中村 仁美, 高木 哲, 武井 好三, 青木 由徳, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 54 (8) 450 -450 2010年08月 [査読無し][通常論文]
  • 犬のガストリノーマに対してサンドスタチンLARを投与した1例
    金子 一成, 細谷 謙次, 滝口 満善, 中村 健介, 北村 二郎, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 54 (8) 450 -450 2010年08月 [査読無し][通常論文]
  • 犬脾臓線維組織球結節の肝転移症例の1治療例
    細谷 謙次, 石崎 恵子, 山崎 裕毅, 金 尚昊, 大田 寛, 森 伸介, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 54 (8) 451 -451 2010年08月 [査読無し][通常論文]
  • 骨盤切除を行った犬の2症例
    青木 由徳, 高木 哲, 大崎 智弘, 細谷 謙次, 千葉 依里, 奥村 正裕 北海道獣医師会雑誌 54 (8) 451 -451 2010年08月 [査読無し][通常論文]
  • 尿路拡張用バルーンカテーテルを用いて治療した尿道腫瘍の犬の1例
    金 尚昊, 高木 哲, 小儀 直子, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 54 (8) 453 -453 2010年08月 [査読無し][通常論文]
  • 犬と猫における閉鎖式吸引ドレナージの有用性
    渡邉 武史, 高木 哲, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 54 (8) 453 -453 2010年08月 [査読無し][通常論文]
  • 胆嚢粘液嚢腫の犬19症例の回顧的解析
    睦良田 尚充, 高木 哲, 細谷 謙次, 中村 健介, 大田 寛, 山崎 真大, 滝口 満喜, 佐藤 耕太, 奥村 正裕 北海道獣医師会雑誌 54 (8) 462 -462 2010年08月 [査読無し][通常論文]
  • 腸瘻チューブによる栄養管理を実施した犬猫15症例の検討
    山崎 裕毅, 高木 哲, 須永 隆文, 青木 由徳, 細谷 謙次, 奥村 正裕 北海道獣医師会雑誌 54 (8) 466 -466 2010年08月 [査読無し][通常論文]
  • 膝蓋骨内方脱臼 関節周囲軟部組織の処理
    奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.1) 128 -130 2010年07月 [査読無し][通常論文]
  • 片側骨盤切除を行った腸骨原発の骨肉腫の一例
    高木 哲, 細谷 謙次, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.1) 270 -270 2010年07月 [査読無し][通常論文]
  • 肘関節に発生したガングリオンの猫の一例
    青木 由徳, 高木 哲, 細谷 謙次, 小儀 直子, 木村 亨史, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.1) 275 -275 2010年07月 [査読無し][通常論文]
  • 血管拡張型骨肉腫と診断された犬の3症例
    青木 由徳, 高木 哲, 細谷 謙次, 須永 隆文, 大崎 智弘, 落合 謙爾, 梅村 孝司, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.1) 332 -332 2010年07月 [査読無し][通常論文]
  • イヌ肥満細胞腫に対するCTを用いた計画的広範囲切除 2症例
    細谷 謙次, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.1) 333 -333 2010年07月 [査読無し][通常論文]
  • 再発性空腸肉芽腫に対して空腸側々吻合術を行ったミニチュアダックスフントの1症例
    大崎 智弘, 田中 里加子, 小川 修治, 山崎 真大, 寸田 裕嗣, 梅村 孝司, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 41 (Suppl.1) 335 -335 2010年07月 [査読無し][通常論文]
  • 【関節鏡 犬の関節疾患の診断・治療に有用な低侵襲性機器】 関節鏡の基本と有用性
    奥村 正裕 SURGEON 14 (3) 6 -13 2010年05月 [査読無し][通常論文]
  • 犬の恥骨結合癒合術 股関節形成異常に対する予防的手術の紹介
    奥村 正裕 北海道獣医師会雑誌 54 (5) 187 -192 2010年05月 [査読無し][通常論文]
     
    イヌの股関節形成異常に対し保存的管理或いは手術療法が行われているが、病態発現予防法として、1990年代終りに恥骨結合癒合術(JPS)が報告された。JPSの実際、JPSによる骨盤変形に関する知見、JPSの適用時期に関する知見、JPSの成果に関する知見を紹介した。JPSに関連する知見は極めて限られているが、適合症例に実施した場合、比較的良好な結果が得られている。
  • Vivien Lettry, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura JAPANESE JOURNAL OF VETERINARY RESEARCH 58 (1) 5 -15 2010年05月 [査読無し][通常論文]
     
    Bone marrow derived mesenchymal stem cells (MSCs) can be used to repair articular cartilage defects, these cells should be properly stimulated so that they could differentiate morphologically and hold cellular synthetic features closer to maturely differentiated chondrocytes. It is well known that tissue specific environment plays an important role in cell fate determination. Once improved isolation, proliferation and differentiation protocols have been developed, the likelihood of spontaneous differentiation of MSCs into divergent lineages will be reduced, thus increasing their value for cartilage repair. The purpose of this study was to improve chondrogenic differentiation of equine MSCs using coculture with mature equine articular chondrocytes (ACs), along with the determination of the effect of adding transforming growth factor (TGF) beta 1 in the pellet culture system. Following confirmation of multilineage (adipogenic, osteogenic and chondrogenic) differentiation, isolated MSCs, ACs and coculture of both cell types were transferred into pellet culture system in a DMEM-based medium supplemented with or without TGF beta 1. Chondrogenic differentiation was evaluated histologically and the relative mRNA expressions of collagen type 1 alpha 1 (COL1A1), collagen type 2 alpha 1(COL2A1), aggrecan (ACAN) and SRY-box 9 (SOX9) were estimated by quantitative RT-PCR. Cocultured cells showed diffuse distribution of extracellular matrix (ECM), whereas in chondrocyte pellets it was more localized to central regions. Expression of COL2A1, ACAN and SOX9 genes were higher in cocultured pellets when compared to MSCs and ACs-composed pellets. Addition of TGF beta 1 in chondrogenic differentiating medium did not consistently amplify expression of the above mentioned genes. Differentiation of equine MSCs was enhanced by coculturing in association with mature ACs, improving expression of cartilage-specific genes and producing a more homogeneous production of ECM within the newly formed cocultured cartilage. The use of the coculture system could possibly enhance the capacity of MSC-derived chondrocytes to build up stable articular cartilage-like constructs, which could play an important role in articular cartilage repair and regeneration.
  • 培養馬滑膜および軟骨細胞における多硫酸化グリコサミノグリカンの抗炎症効果発現機序
    住江 康晴, 須永 隆文, 光田 健太, 金 尚昊, 細谷 謙次, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 149回 286 -286 2010年03月 [査読無し][通常論文]
  • 犬培養滑膜細胞に対するリポキシゲナーゼ阻害薬AA861のMAPK経路への効果およびアポトーシス誘導作用
    須永 隆文, 住江 康晴, 細谷 謙次, 高木 哲, 奥村 正裕 日本獣医学会学術集会講演要旨集 149回 288 -288 2010年03月 [査読無し][通常論文]
  • 犬脂肪由来ストローマ細胞の分離および性状に関する研究
    日浅 真美, 高木 哲, 細谷 謙次, 奥村 正裕 日本獣医学会学術集会講演要旨集 149回 290 -290 2010年03月 [査読無し][通常論文]
  • Vivien Lettry, Yasuharu Sumie, Kenta Mitsuda, Masaaki Tagami, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura JAPANESE JOURNAL OF VETERINARY RESEARCH 57 (4) 197 -206 2010年02月 [査読無し][通常論文]
     
    The objective of this study was to investigate the changes in synovial fluid concentration of collagen type 11 cleavage site (C2C) and procollagen II C-propeptide (CPII), markers of joint cartilage degeneration and synthesis, respectively, in horses with intraarticular fracture or osteochondrosis dissecans (OCD), and to examine the relationship between arthroscopic findings and these biomarker levels. Synovial fluid was collected from 36 joints in 18 horses (6 fractures and 12 OCDs). Samples from contralateral normal joints, when available, served as controls (n = 12). Concentrations of C2C and CPII were measured using enzyme-linked immunosorbant assays. Moreover, the severity of the cartilage degradation was graded arthroscopically in 16 horses, and the correlation between the C2C and CPII levels and the arthroscopic scores were investigated. Compared to the control, the concentration of C2C was increased in OCD joints but not in fracture joints, whereas the concentration of CPII was increased in fracture joints but not in OCD joints. Within each disease group there was no correlation between biomarker levels and arthroscopic findings. Therefore, although C2C and CPII have diagnostic potential further knowledge is required to provide accurate analysis.
  • Vivien Lettry, Hirofumi Kawasaki, Kiyofumi Sugaya, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura JAPANESE JOURNAL OF VETERINARY RESEARCH 57 (4) 207 -212 2010年02月 [査読無し][通常論文]
     
    This study aimed to evaluate a system that identifies cartilage turn over and/or degradation through measurement of a new keratan sulfate (KS) epitope concentration in equine sera. Blood samples were collected from 30 horses, 1 (n = 15) and 2 year-olds (n = 15). Serum samples were analyzed for an epitope of keratan sulfate by 1/20/5D4 (KS5D4) and new epitopes of keratan sulfate using high sensitive keratan sulfate (HSKS), measured by two respective enzyme-linked immunosorbant assays (ELISAs). There was no correlation in serum concentration of KS evaluated using 5D4 and HSKS. Age had no significant effect on concentrations of KS measured with KS5D4 while 1 year-old horses showed significantly higher amounts than 2 year-olds with HSKS. Results suggest that HSKS could detect early signs of cartilage metabolic changes.
  • 外科手術を実施した犬の肝細胞癌17症例の検討
    高木 哲, 滝口 満喜, 山崎 真大, 大田 寛, 中村 健介, 大崎 智弘, 細谷 謙次, 奥村 正裕 獣医麻酔外科学雑誌 40 (Suppl.2) 196 -196 2009年12月 [査読無し][通常論文]
  • 外科的摘出が困難な上顎腫瘍に対して光線力学療法を行った犬の4症例
    大崎 智弘, 高木 哲, 細谷 謙次, 奥村 正裕 獣医麻酔外科学雑誌 40 (Suppl.2) 197 -197 2009年12月 [査読無し][通常論文]
  • 光感受性物質Benzoporphyrin derivative monoacid ring A(BPD-MA)腫瘍組織内投与における体組織分布
    王 禎祥, 高木 哲, 大崎 智弘, 須永 隆文, 奥村 正裕 獣医麻酔外科学雑誌 40 (Suppl.2) 197 -197 2009年12月 [査読無し][通常論文]
  • 肘関節脱臼を経関節ピンにより整復した犬の2症例
    青木 由徳, 高木 哲, 細谷 謙次, 大崎 智弘, 奥村 正裕 獣医麻酔外科学雑誌 40 (Suppl.2) 200 -200 2009年12月 [査読無し][通常論文]
  • 犬の骨関節疾患の診断と治療 犬用NSAIDズブリンの特徴と関節症治療における可能性
    奥村 正裕 獣医麻酔外科学雑誌 40 (Suppl.2) 240 -240 2009年12月 [査読無し][通常論文]
  • 交通事故による膀胱・尿道損傷により尿路変更を行った犬の1例
    高木 哲, 須永 隆文, 小儀 直子, 青木 由徳, 寺本 英司, 大崎 智弘, 奥村 正裕 動物臨床医学会年次大会プロシーディング 30回 (2) 163 -164 2009年11月 [査読無し][通常論文]
  • 日本の小動物臨床獣医師における動物の痛みとその治療に関する意識の変化 2004年および2008年に実施したアンケート結果の比較
    今井 彩子, 山下 和人, 西村 亮平, 左近允 巌, 永延 清和, 奥村 正裕, 枝村 一弥, 浅川 誠, 高島 一昭, 山根 義久 動物臨床医学会年次大会プロシーディング 30回 (3) 180 -181 2009年11月 [査読無し][通常論文]
  • 犬の慢性骨関節疾患の診断と治療 犬の慢性痛評価基準の作成
    左近允 巌, 浅川 誠, 今井 彩子, 枝村 一弥, 奥村 正裕, 永延 清和, 西村 亮平, 山下 和人, 高島 一昭, 山根 義久 動物臨床医学会年次大会プロシーディング 30回 (3) 188 -192 2009年11月 [査読無し][通常論文]
  • 犬の慢性骨関節疾患の診断と治療 四肢
    奥村 正裕 動物臨床医学会年次大会プロシーディング 30回 (3) 193 -197 2009年11月 [査読無し][通常論文]
  • "歩き方が変ですけど・・・"を治すために 犬の骨関節炎の診断と治療
    奥村 正裕 動物臨床医学会年次大会プロシーディング 30回 (3) 248 -250 2009年11月 [査読無し][通常論文]
  • 犬の変形性関節症の薬物療法と非ステロイド性抗炎症薬 犬用コキシブ系薬剤フィロコキシブの臨床的有用性について
    奥村 正裕 MVM 18 (6) 63 -68 2009年09月 [査読無し][通常論文]
  • 犬関節症における関節内投与ヒアルロン酸の治療効果とその発現機構
    奥村 正裕, 須永 隆史, 住江 康晴, 高木 哲 北海道獣医師会雑誌 53 (8) 80 -80 2009年08月 [査読無し][通常論文]
  • 滑膜炎の初期管理にステロイドの関節内投与を行った軽度肩関節不安定症の2症例
    千葉 依里, 高木 哲, 大崎 智弘, 奥村 正裕 北海道獣医師会雑誌 53 (8) 80 -80 2009年08月 [査読無し][通常論文]
  • 腸間膜リンパ節から胸管造影を行った乳び胸の犬の1例
    小儀 直子, 高木 哲, 井上 愛香, 須永 隆文, 大崎 智弘, 奥村 正裕 北海道獣医師会雑誌 53 (8) 88 -88 2009年08月 [査読無し][通常論文]
  • 小腸の再発性肉芽腫に対して十二指腸空腸吻合術を行ったミニチュアダックスフントの1症例
    大崎 智弘, 小川 修治, 山崎 真大, 寸田 裕嗣, 梅村 孝司, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 53 (8) 90 -90 2009年08月 [査読無し][通常論文]
  • 骨盤弁形成骨切り術を行い直腸に発生した平滑筋腫を切除した犬の1例
    日浅 真美, 高木 哲, 植田 篤樹, 大崎 智弘, 奥村 正裕 北海道獣医師会雑誌 53 (8) 90 -90 2009年08月 [査読無し][通常論文]
  • 交通事故による尿道断裂に対して膀胱全摘出および尿路再建術を実施した犬1症例
    須永 隆文, 高木 哲, 小儀 直子, 青木 由徳, 寺本 英司, 大崎 智弘, 奥村 正裕 北海道獣医師会雑誌 53 (8) 91 -91 2009年08月 [査読無し][通常論文]
  • 血管拡張型骨肉腫と診断された犬の3症例
    青木 由徳, 高木 哲, 須永 隆文, 大崎 智弘, 寸田 祐嗣, 落合 謙爾, 梅村 孝司, 奥村 正裕 北海道獣医師会雑誌 53 (8) 91 -91 2009年08月 [査読無し][通常論文]
  • 胆嚢粘液嚢腫の犬5例における周術期管理
    光田 健太, 高木 哲, 須永 隆文, 滝口 満喜, 大田 寛, 佐藤 耕太, 大崎 智弘, 奥村 正裕 北海道獣医師会雑誌 53 (8) 93 -93 2009年08月 [査読無し][通常論文]
  • 副腎皮質機能低下症の犬2症例に対する周術期管理
    住江 康晴, 高木 哲, 須永 隆文, 青木 由徳, 千葉 依里, 久万田 剛, 千石 和彦, 奥村 正裕 北海道獣医師会雑誌 53 (8) 94 -94 2009年08月 [査読無し][通常論文]
  • 奥村 正裕 獣医麻酔外科学雑誌 = Japanese journal of veterinary anesthesia & surgery 40 (Suppl.1) 89 -91 2009年06月20日 [査読無し][通常論文]
  • 奥村 正裕 獣医麻酔外科学雑誌 = Japanese journal of veterinary anesthesia & surgery 40 (Suppl.1) 230 -231 2009年06月20日 [査読無し][通常論文]
  • 副腎皮質機能低下症の犬2例における周術期管理
    高木 哲, 須永 隆文, 青木 由徳, 久万田 剛, 千石 和彦, 奥村 正裕 獣医麻酔外科学雑誌 40 (Suppl.1) 177 -177 2009年06月 [査読無し][通常論文]
  • マウスおよび犬の腫瘍に対するPDTの血管遮蔽効果の臨床学的評価
    大崎 智弘, 高木 哲, 奥村 正裕 獣医麻酔外科学雑誌 40 (Suppl.1) 188 -188 2009年06月 [査読無し][通常論文]
  • 浅指屈筋に発生した筋肉内血管腫を疑った犬の一例
    青木 由徳, 高木 哲, 日浅 真美, 落合 謙爾, 大崎 智弘, 梅村 孝司, 奥村 正裕 獣医麻酔外科学雑誌 40 (Suppl.1) 192 -192 2009年06月 [査読無し][通常論文]
  • 星野 有希, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 日本獣医師会雑誌 62 (5) 383 -387 2009年05月20日 [査読無し][通常論文]
     
    悪性腫瘍罹患犬10症例に対して活性化リンパ球療法を実施した。症例犬より採取した末梢血単核球を抗CD3抗体およびヒトリコンビナントIL-2を用いて14日間培養後、ヒトリコンビナントIFN-αを感作した細胞を活性化リンパ球とし、当該症例に複数回投与した。その結果、すべての症例で末梢血単核球細胞の構成細胞比が変化し、うち2症例で血清IFN-γ濃度の上昇が認められた。また活性化リンパ球の投与による副作用は認められず、症例の生活の質を十分維持することが可能であった。以上のことから、本治療法は腫瘍の発症およびその治療により生活の質が低下しがちな犬に対しても免疫応答を活性化することが可能であり、腫瘍の成長および転移に対する免疫学的防御能を活性化させる治療法として十分適用可能であると考えられた。
  • 前十字靱帯断裂に対する治療法 生体力学的膝関節安定化術
    奥村 正裕 北海道獣医師会雑誌 53 (5) 209 -214 2009年05月 [査読無し][通常論文]
  • 高木 哲, 須永 隆文, 山田 佳代子, 青木 由徳, 大崎 智弘, 奥村 正裕 動物臨床医学 18 (1) 11 -15 2009年03月20日 [査読無し][通常論文]
  • 獣医学における腫瘍研究最前線 活性化リンパ球療法および樹状細胞ワクチン療法の臨床応用
    高木 哲, 星野 有希, 須永 隆文, 大崎 智弘, 奥村 正裕 日本獣医学会学術集会講演要旨集 147回 163 -163 2009年03月 [査読無し][通常論文]
  • 犬の自然発生悪性黒色腫における腫瘍関連抗原gp100の発現とそれを用いたペプチドワクチンの免疫学的効果
    山田 佳代子, 高木 哲, 須永 隆文, 奥村 正裕 日本獣医学会学術集会講演要旨集 147回 272 -272 2009年03月 [査読無し][通常論文]
  • Tomohiro Osaki, Satoshi Takagi, Yuki Hoshino, Masahiro Okumura, Tsuyoshi Kadosawa, Toru Fujinaga The Journal of veterinary medical science 71 (2) 125 -32 2009年02月 [査読無し][通常論文]
     
    Antivascular photodynamic therapy (PDT) suppresses tumor growth and prolonged the survival in solid tumor-bearing mice. The purpose of this study was to assess the efficacy of antivascular PDT using BPD-MA for treatment of oral and nasal tumors in 14 dogs. At 15 min after initiating intravenous infusion of 0.5 mg/kg benzoporphyrin derivative monoacid ring A, tumors were irradiated with laser light at 690 nm emitted by a diode laser. The 1-year survival rate of 7 dogs with oral tumors was 71%. The 1-year survival rate of 7 dogs with nasal tumors was 57%. Imaging of each tumor was performed by using angiographic computed tomography before and after each antivascular PDT. Contrast-enhanced tumors were observed before antivascular PDT, but these tumors were not enhanced with contrast medium following antivascular PDT. Antivascular PDT is suggested to be a promising method for dogs with oral and nasal tumors that cannot be effectively treated with current antitumor therapies.
  • 高木 哲, 須永 隆文, 星野 有希, 唯野 剛史, 梅村 孝司, 大崎 智弘, 寺本 英司, 奥村 正裕 獣医麻酔外科学雑誌 = Japanese journal of veterinary anesthesia & surgery 39 (3/4) 51 -56 2009年01月30日 [査読無し][通常論文]
     
    2歳齢の雌のミニチュアシュナウザーが左大腿骨骨折を主訴に来院した。禀告から1歳前後での複数回の骨折歴を聴取し、X線検査で全身骨格において著しいデンシティーの低下を認めた。各種血液検査によって代謝性骨疾患の可能性が否定され、骨の病理組織学的検査所見などから骨形成不全症(Osteogenesis Imperfecta:OI)の可能性が推測された。(著者抄録)
  • A new high sensitive keratin sulfate biomarker to detect metabolic changes of cartilage in horses.
    Proceedings of 11th World congress of World equine veterinary association 1 2009年 [査読無し][通常論文]
  • 著しい骨脆弱を認めた若齢犬の1例
    高木 哲, 寺本 英司, 須永 隆文, 大崎 智弘, 奥村 正裕 動物臨床医学会年次大会プロシーディング 29回 (2) 251 -252 2008年11月 [査読無し][通常論文]
  • 【関節液と関節鏡検査のプロトコール】 犬関節疾患の診断における関節鏡の有用性
    奥村 正裕 CAP: Companion Animal Practice 23 (9) 18 -26 2008年09月 [査読無し][通常論文]
  • 犬口腔悪性黒色腫に対する樹状細胞ワクチン療法の基礎的試験と臨床例の検討
    高木 哲, 大薗 三千代, 鮎沢 小百合, 小森谷 薫, 相川 裕子, 坂本 美智子, 天辻 康夫, 大野 典也, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 146回 252 -252 2008年09月 [査読無し][通常論文]
  • 犬培養滑膜細胞におけるリポキシゲナーゼ、RhoおよびプロスタグランジンE2合成酵素遺伝子検出法の最適化
    須永 隆文, 奥村 正裕, 高木 哲 日本獣医学会学術集会講演要旨集 146回 264 -264 2008年09月 [査読無し][通常論文]
  • 生前診断が困難であった血管肉腫の犬の1例
    須永 隆文, 高木 哲, 嶋本 良則, 梅村 孝司, 奥村 正裕 北海道獣医師会雑誌 52 (8) 414 -414 2008年08月 [査読無し][通常論文]
  • 播種性組織球肉腫の猫1症例
    大崎 智弘, 田中 里加子, 唯野 剛, 落合 謙爾, 梅村 孝司, 高木 哲, 奥村 正裕 北海道獣医師会雑誌 52 (8) 414 -414 2008年08月 [査読無し][通常論文]
  • 肝細胞癌摘出後に多発性転移を認めた犬の2例
    高木 哲, 山崎 真大, 須永 隆文, 小川 修治, 落合 謙爾, 梅村 孝司, 奥村 正裕 北海道獣医師会雑誌 52 (8) 416 -416 2008年08月 [査読無し][通常論文]
  • 骨転移を生じた肺原発性腺扁平上皮癌の犬の一例
    小川 修治, 高木 哲, 近藤 厚, 高橋 徹, 山田 佳代子, 奥村 正裕 北海道獣医師会雑誌 52 (8) 416 -416 2008年08月 [査読無し][通常論文]
  • 外傷性横隔膜ヘルニアを呈した犬の1例
    山田 佳代子, 高木 哲, 須永 隆文, 青木 由徳, 本田 英隆, 奥村 正裕 北海道獣医師会雑誌 52 (8) 419 -419 2008年08月 [査読無し][通常論文]
  • 前肢の広範な皮膚欠損創に人工真皮を適用した犬の1症例
    小川 修治, 高木 哲, 田中 里加子, 奥村 正裕 北海道獣医師会雑誌 52 (7) 269 -272 2008年07月 [査読無し][通常論文]
  • 星野 有希, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 The journal of veterinary medical science 70 (6) 581 -588 2008年06月25日 [査読無し][通常論文]
     
    活性化リンパ球療法の小動物腫瘍症例への臨床応用のため次のことについて検討した.健常犬の末梢血単核球(PBMC)を抗CD3抗体とインターロイキン(IL)-2を用いて14日間培養して活性化させた後,インターフェロン(IFN)-αとIL-2により再活性化させた細胞について性状を解析してPBMCと比較した.その結果,培養によって得られた細胞は,PBMCと比較すると50倍以上に増加し,CD8^+細胞が顕著に増加し,細胞傷害能力の増強とCD56mRNA量の増加が認められ,リンパ球が活性化されたことが確認された.次いでこの活性化リンパ球を健常犬に反復投与したところ,細胞増殖活性の上昇,PBMC中のCD4^+細胞,CD8^+細胞の増加および血清IFN-γ濃度の上昇が認められた.このことより細脆性免疫応答の活性化が起こったものと推測された.また,特に副作用と思われる反応は認められなかった.以上のことから,健常犬において抗CD3抗体とIL-2により活性化リンパ球の誘導が可能であること,およびその活性化リンパ球を反復投与することによって免疫応答の活性化が起こることが示唆された.
  • 奥村 正裕 獣医麻酔外科学雑誌 = Japanese journal of veterinary anesthesia & surgery 39 (Suppl.1) 61 -63 2008年06月20日 [査読無し][通常論文]
  • 犬の腫瘍治療における免疫療法:活性化リンパ球療法
    星野 有希, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 52 (6) 212 -218 2008年06月 [査読無し][通常論文]
  • 星野 有希, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 The Journal of Veterinary Medical Science 70 (6) 581 -588 2008年06月 [査読無し][通常論文]
     
    We attempted to accumulate the basic data for evaluation of activated lymphocyte therapy for small animal medicine. The peripheral blood mononuclear cells (PBMCs) collected from healthy dogs were activated using anti-CD3 antibody and human recombinant (hr) interleukin (lL)-2 and reactivated using hr interferon (IFN)-alpha and hr IL-2. The property of obtained cells was compared with PBMCs. The number of cells was shown to have increased approximately > 50-fold by cultivation. The proportion of CD8(+) cells was significantly increased, the cytotoxicity of the cultured cells was revealed to have been reinforced. Additionally, CD56 mRNA levels tended to have increased. The cells obtained by this method were confirmed to be activated lymphocytes. Furthermore, we investigated the effects of sequential administration of the obtained cells to healthy dogs. By sequential administration of the activated lymphocytes, the cell proliferative activity, proportion of CD4(+) cells and CD8(+) cells, and serum IFN-gamma concentration were shown to have increased, and no severe adverse effects were observed. Consequently, activated lymphocytes could be induced using anti-CD3 antibody and IL2 in healthy dogs, and sequential administration of activated lymphocytes reinforced the recipient's immunity.
  • 前肢の広範な皮膚欠損創に人工真皮を適用した犬の1症例
    高木 哲, 小川 修治, 奥村 正裕 獣医麻酔外科学雑誌 39 (Suppl.1) 196 -196 2008年06月 [査読無し][通常論文]
  • 慢性疼痛のサイン 飼い主の"何か変"は痛みの表現
    山下 和人, 左近允 巌, 奥村 正裕, Nolan Andrea MVM 17 (4) 53 -57 2008年05月 [査読無し][通常論文]
  • 日本における動物の慢性痛管理 現状から未来を見据えて
    山下 和人, 西村 亮平, 今井 彩子, 奥村 正裕, 永延 清和, Downing Robin, Grubb Tamara CLINIC NOTE 4 (4) 54 -59 2008年04月 [査読無し][通常論文]
  • 星野 有希, 田島 誉士, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 The Journal of Veterinary Medical Science 70 (3) 309 -312 2008年03月 [査読無し][通常論文]
     
    Real-time PCR was optimized for the quantification of canine CD56 mRNA expression. This study was conducted to easily quantify canine CD56 expression and to identify its expression in normal tissues, peripheral blood mononuclear cells and activated lymphocytes in dogs. This assay revealed the highest level of CD56 mRNA expression in the normal canine brain, followed by the lung, kidney and liver. CD56 mRNA expression level in peripheral blood mononuclear cells was considerably lower; among activated lymphocytes in vitro, CD56 mRNA expression was increased.
  • In vitro犬関節滑膜および軟骨細胞における外因性ヒアルロン酸および多硫酸化グリコサミノグリカンのヒアルロン酸産生効果
    溝部 文彬, 奥村 正裕, 須永 隆文, 高木 哲, 藤永 徹 日本獣医学会学術集会講演要旨集 145回 216 -216 2008年03月 [査読無し][通常論文]
  • 自然発生悪性腫瘍罹患犬6例に適用した樹状細胞(DC)療法の有効性
    鮎沢 小百合, 高木 哲, 大薗 三千代, 小森谷 薫, 相川 裕子, 天辻 康夫, 大野 典也, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 145回 220 -220 2008年03月 [査読無し][通常論文]
  • 犬における腫瘍マーカーとしてのミッドカインの有用性
    高木 哲, 大薗 三千代, 天辻 康夫, 大野 典也, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 145回 221 -221 2008年03月 [査読無し][通常論文]
  • Benzoporphyrin derivative monoacid ring A(BPD-MA)マウス腫瘍内投与による光線力学療法の有用性に関する基礎的検討
    宇原 丈順, 奥村 正裕, 高木 哲, 藤永 徹 日本獣医学会学術集会講演要旨集 145回 222 -222 2008年03月 [査読無し][通常論文]
  • 星野 有希, 田島 誉士, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 The journal of veterinary medical science 70 (3) 309 -12 2008年03月 [査読無し][通常論文]
     
    犬のCD56mRNA発現について調べるためにリアルタイムPCRを用いて比較的簡便なCD56mRNA発現量の相対定量法を確立し,犬の正常臓器,末梢血単核球,活性化したリンパ球のCD56mRNA発現量を調べた.その結果,正常臓器では脳が最も高く,肺,肝臓および腎臓にも認められた.末梢血単核球のCD56mRNA発現量は低かったが,in vitoで活性化したリンパ球では発現量が増加した.
  • 【小動物外科のトレンド】 前十字靱帯断裂に対する治療 生体力学的安定化術(TPLO、TTA)は問題を解決できるか
    奥村 正裕 獣医畜産新報 60 (12) 999 -1004 2007年12月 [査読無し][通常論文]
  • 【生検・細胞診の手技 後編】 関節疾患における生検・細胞診の手技 関節液の検査と滑膜生検の手技
    奥村 正裕 SA Medicine 9 (6) 33 -41 2007年12月 [査読無し][通常論文]
  • 骨再生 骨形成タンパクBone morphogenic proteinによる骨再生 その作用と最新知見
    奥村 正裕 獣医麻酔外科学雑誌 38 (Suppl.2) 79 -82 2007年11月 [査読無し][通常論文]
  • 犬の関節症と関節炎・臨床診断と治療のプロトコール
    奥村 正裕 動物臨床医学会年次大会プロシーディング 28回 (1) 3 -7 2007年11月 [査読無し][通常論文]
  • 成長期犬の前肢姿勢異常 Carpal Laxity Syndromeの犬2例
    奥村 正裕, 鮎沢 小百合, 星野 有希, 高木 哲, 藤永 徹 動物臨床医学会年次大会プロシーディング 28回 (2) 381 -382 2007年11月 [査読無し][通常論文]
  • 犬の慢性痛評価基準の作成(経過報告)
    左近允 巌, 浅川 誠, 今井 彩子, 枝村 一弥, 奥村 正裕, 永延 清和, 西村 亮平, 山下 和人, 高島 一昭, 山根 義久, 動物のいたみ研究会 動物臨床医学会年次大会プロシーディング 28回 (3) 181 -185 2007年11月 [査読無し][通常論文]
  • 新世代のコキシブ系犬用NSAIDの臨床応用について
    奥村 正裕 動物臨床医学会年次大会プロシーディング 28回 (3) 218 -218 2007年11月 [査読無し][通常論文]
  • 犬関節症に対する非ステロイド性抗炎症薬の使用 非ステロイド性抗炎症薬ニメスリドの紹介
    奥村 正裕 CLINIC NOTE 3 (11) 48 -56 2007年11月 [査読無し][通常論文]
  • 無水アルコール抽出成分を補強した緑イ貝抽出物の犬関節症における臨床的効果
    奥村 正裕 MVM 16 (7) 67 -71 2007年11月 [査読無し][通常論文]
  • 血管炎に起因したと考えられる悪性関節リウマチの犬1症例
    宇原 丈順, 奥村 正裕, 小川 修治, 鮎沢 小百合, 溝部 文彬, 高木 哲, 藤永 徹 北海道獣医師会雑誌 51 (8) 319 -319 2007年08月 [査読無し][通常論文]
  • 腫瘍切除後の広範な皮膚欠損にアテロコラーゲン人工真皮を適用した犬の1症例
    小川 修治, 高木 哲, 宇原 丈順, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 51 (8) 325 -325 2007年08月 [査読無し][通常論文]
  • 成長に関連して手根関節拘縮を呈した犬の2症例
    鮎沢 小百合, 奥村 正裕, 小川 修二, 宇原 丈順, 溝部 文彬, 高木 哲, 藤永 徹 北海道獣医師会雑誌 51 (8) 329 -329 2007年08月 [査読無し][通常論文]
  • 潜在的な食道機能低下による臨床症状を呈したと考えられた犬の3症例
    溝部 文彬, 高木 哲, 奥村 正裕, 小川 修治, 鮎沢 小百合, 宇原 丈順, 藤永 徹 北海道獣医師会雑誌 51 (8) 334 -334 2007年08月 [査読無し][通常論文]
  • 動物を犠牲にしない獣医学教育の実践 東南アジア/オセアニア諸国の獣医学教育
    橋本 善春, 昆 泰寛, 奥村 正裕, 伊藤 茂男 日本獣医学会学術集会講演要旨集 144回 23 -23 2007年08月 [査読無し][通常論文]
  • 犬培養滑膜線維芽細胞に対するリポキシゲナーゼ(LOX)阻害薬のアポトーシス誘導作用
    須永 隆文, 奥村 正裕, 溝部 文彬, 田崎 竜也, 星野 有希, 高木 哲, 藤永 徹 日本獣医学会学術集会講演要旨集 144回 158 -158 2007年08月 [査読無し][通常論文]
  • 動物の腫瘍に対するBPD-MA(benzoporphyrin derivative monoacid ring A)を用いた光線力学療法の基礎的および臨床学的研究
    大崎 智弘, 高木 哲, 星野 有希, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 143回 65 -65 2007年03月 [査読無し][通常論文]
  • 競走馬の獣医師の挑戦 最大限のパフォーマンスを求めて 競走馬の運動器を守る(1) 骨格・関節への負担の理解とその緩和
    奥村 正裕 日本獣医学会学術集会講演要旨集 143回 78 -78 2007年03月 [査読無し][通常論文]
  • 硬性鏡の現状と将来展望 小動物の関節鏡
    奥村 正裕 日本獣医学会学術集会講演要旨集 143回 127 -127 2007年03月 [査読無し][通常論文]
  • 歯髄炎を発症したセイウチ上顎犬歯(牙)の摩耗面に関する電顕観察
    橋本 善春, 昆 泰寛, 高木 哲, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 143回 151 -151 2007年03月 [査読無し][通常論文]
  • BPD-MA(Benzoporphyrin derivative monoacid ring A)の腫瘍内投与による光線力学療法の抗腫瘍効果
    小松 広宗, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 143回 211 -211 2007年03月 [査読無し][通常論文]
  • 犬腫瘍細胞溶解産物を感作した樹状細胞ワクチンの有用性に関する基礎的研究
    横川 美穂, 今内 覚, 星野 有希, 高木 哲, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 143回 211 -211 2007年03月 [査読無し][通常論文]
  • 犬の移行上皮癌細胞に対する非ステロイド性抗炎症薬(NSAIDs)の増殖抑制効果とその作用機序の解析
    田崎 竜也, 高木 哲, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 143回 212 -212 2007年03月 [査読無し][通常論文]
  • 犬末梢血単核球におけるCD56mRNA発現とその定量法の確立
    星野 有希, 高木 哲, 木村 享史, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 143回 217 -217 2007年03月 [査読無し][通常論文]
  • 星野有希, 高木哲, 大崎智弘, 奥村正裕, 藤永徹 日本獣医師会三学会年次大会講演要旨集 2006 208 2007年 [査読無し][通常論文]
  • 動物を犠牲にしない獣医学教育の試み EU圏の獣医解剖学と獣医外科学教育の現状について
    橋本 善春, 昆 泰寛, 奥村 正裕, 伊藤 茂男 臨床獣医 24 (12) 68 -75 2006年12月 [査読無し][通常論文]
  • 【肘関節の外科】 肘関節における非炎症性関節症
    奥村 正裕 SURGEON 10 (6) 50 -55 2006年11月 [査読無し][通常論文]
  • 【肘関節の外科】 炎症性関節症
    奥村 正裕 SURGEON 10 (6) 56 -61 2006年11月 [査読無し][通常論文]
  • リンパ節転移巣切除およびメルファラン投与を行った肛門嚢腺癌の犬の1例
    高木 哲, 星野 有希, 奥村 正裕, 田島 誉士, 藤永 徹 獣医麻酔外科学雑誌 37 (Suppl.2) 233 -233 2006年11月 [査読無し][通常論文]
  • 脊髄圧迫に関連した硬膜病変を呈した硬膜外脊髄脂肪腫の犬1症例
    奥村 正裕, 須永 隆文, 田崎 竜也, 星野 有希, 梅村 孝司, 高木 哲, 藤永 徹 動物臨床医学会年次大会プロシーディング 27回 (2) 87 -88 2006年11月 [査読無し][通常論文]
  • 関節疾患診断・治療のプロトコール 関節穿刺の方法と活用法
    奥村 正裕 動物臨床医学会年次大会プロシーディング 27回 (1) 59 -67 2006年11月 [査読無し][通常論文]
  • 犬の腫瘍症例に対する「アガリクス茸+サメ軟骨2」 給餌の臨床学的効果
    高木 哲, 奥村 正裕, 島田 健次郎, 藤永 徹 小動物臨床 25 (5) 346 -353 2006年09月 [査読無し][通常論文]
  • 頸部リンパ節に原発不明の悪性黒色腫を認めた犬の1症例
    高木 哲, 星野 有希, 大崎 智弘, 落合 謙爾, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 50 (9) 404 -407 2006年09月 [査読無し][通常論文]
  • 光線力学療法により治療を試みたリンパ球形質細胞性鼻炎の犬の1例
    大崎 智弘, 高木 哲, 櫻庭 義彦, 星野 有希, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 50 (8) 88 -88 2006年08月 [査読無し][通常論文]
  • 活性化自己リンパ球(LAK細胞)療法を行った腫瘍罹患犬6症例
    星野 有希, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 50 (8) 89 -89 2006年08月 [査読無し][通常論文]
  • 部分的膀胱壊死を伴った重度会陰ヘルニアの犬の1症例
    横川 美穂, 奥村 正裕, 星野 有希, 高木 哲, 藤永 徹 北海道獣医師会雑誌 50 (8) 89 -89 2006年08月 [査読無し][通常論文]
  • 脊髄硬膜内外の病変により後肢起立不能を発症した犬の2症例
    須永 隆文, 奥村 正裕, 星野 有希, 梅村 孝司, 田崎 竜也, 高木 哲, 藤永 徹 北海道獣医師会雑誌 50 (8) 93 -93 2006年08月 [査読無し][通常論文]
  • 馬尾神経領域に異常が疑われた犬2症例におけるMRI診断の有効性
    田崎 竜也, 奥村 正裕, 大崎 智弘, 譽田 由香, 星野 有希, 高木 哲, 藤永 徹 北海道獣医師会雑誌 50 (8) 93 -93 2006年08月 [査読無し][通常論文]
  • 変形癒合のみられた大腿骨分筋骨折にインターロッキングネイル固定法(I.I.N.法)を適用した犬の1症例
    小松 広宗, 奥村 正裕, 大崎 智弘, 譽田 由香, 須永 隆文, 高木 哲, 藤永 徹 北海道獣医師会雑誌 50 (8) 95 -95 2006年08月 [査読無し][通常論文]
  • 潜在性二分脊椎症による尿失禁および便失禁を呈したフレンチブルドックの1例
    大田 寛, 高木 哲, 大崎 智弘, 小川 修治, 譽田 由香, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 50 (8) 114 -117 2006年08月 [査読無し][通常論文]
  • 動物を犠牲にしない獣医学教育の実践 EUの獣医学教育
    橋本 善春, 昆 泰寛, 奥村 正裕, 伊藤 茂男 日本獣医学会学術集会講演要旨集 142回 27 -27 2006年08月 [査読無し][通常論文]
  • 大田寛, 高木哲, 大崎智弘, 小川修治, 譽田由香, 奥村正裕, 藤永徹 北海道獣医師会雑誌 50 (8) 370 -373 2006年08月01日 [査読無し][通常論文]
  • 高木 哲, 星野 有希, 奥村 正裕, 田島 誉士, 藤永 徹 獣医麻酔外科学雑誌 = Japanese journal of veterinary anesthesia & surgery 37 2006年07月30日 [査読無し][通常論文]
  • 免疫介在性関節炎の病態・診断・治療について考える 慢性関節リウマチに対する臨床的アプローチ
    奥村 正裕 獣医麻酔外科学雑誌 37 (Suppl.1) 96 -98 2006年06月 [査読無し][通常論文]
  • 関節炎自然発症犬および実験的関節炎作出ラットにおける緑イ貝抽出脂質(GMEL)経口投与効果の臨床学的考察
    奥村 正裕, Heeduck Cho, 高木 哲, 山崎 孝一, 島田 健次郎, 藤永 徹 小動物臨床 25 (3) 188 -198 2006年05月 [査読無し][通常論文]
  • 大崎智弘, 高木哲, 星野有希, 奥村正裕, 廉澤剛, 廉澤剛, 藤永徹 日本獣医師会三学会年次大会講演要旨集 2005 162 2006年 [査読無し][通常論文]
  • いたみのマネージメント 症例別推奨プロトコールについて考える サプリメント サプリメントを活用した臨床報告
    奥村 正裕, 高木 哲, 藤永 徹 動物臨床医学会年次大会プロシーディング 26回 (3) 236 -241 2005年11月 [査読無し][通常論文]
  • 頸部リンパ節に悪性黒色腫の大きな病巣を認めた犬の1例
    高木 哲, 大崎 智弘, 星野 有希, 横山 文香, 田崎 竜也, 落合 謙爾, 奥村 正裕, 藤永 徹 動物臨床医学会第26回年次大会プロシーディング2号 26回 (2) Page259-260 -260 2005年11月 [査読無し][通常論文]
  • 関節炎自然発症犬および実験的関節炎作出ラットにおける緑イ貝抽出物(GMEL)経口投与効果の臨床学的考察
    奥村 正裕, 横山 文香, 高木 哲, 島田 健次郎, 藤永 徹 動物臨床医学会第26回年次大会プロシーディング3号 26回 (3) Page52-56 -56 2005年11月 [査読無し][通常論文]
  • 乳用牛における廃用の要因としての蹄病の意義に関する一考察
    大崎 智弘, 鬼頭 宗寛, 川崎 洋史, 中禮 敏彦, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 49 (9) 1 -9 2005年09月 [査読無し][通常論文]
  • 多発性の皮膚転移が疑われた犬の移行上皮癌の1症例
    高木 哲, 松村 淳, 山崎 真大, 大崎 智弘, 星野 有希, 横川 美穂, 田崎 竜也, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 49 (8) 56 -56 2005年08月 [査読無し][通常論文]
  • 犬の口腔・鼻腔腫瘍の腫瘍血管を標的とした光線力学療法の臨床的効果
    大崎 智弘, 廉澤 剛, 星野 有希, 高木 哲, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 49 (8) 57 -57 2005年08月 [査読無し][通常論文]
  • 四肢関節腫脹を呈した犬1例における閉口障害と炎症性関節症の関連性
    奥村 正裕, 星野 有希, 古橋 由美子, 大崎 智弘, 高木 哲, 藤永 徹 北海道獣医師会雑誌 49 (8) 65 -65 2005年08月 [査読無し][通常論文]
  • 髄内ピンとエポキシパテで固定した中足骨骨折の犬の2症例
    星野 有希, 奥村 正裕, 大崎 智弘, 高木 哲, 藤永 徹 北海道獣医師会雑誌 49 (8) 66 -66 2005年08月 [査読無し][通常論文]
  • 高木 哲, 加藤 友香, 浅野 和之, 大崎 智弘, ダルコ・ボスナコフスキー, 星野 有希, 奥村 正裕, 廉澤 剛, 藤永 徹 The Journal of Veterinary Medical Science 67 (8) 761 -767 2005年08月 [査読無し][通常論文]
     
    Matrix metalloproteinases (MMPs) selectively degrade the extracellular matrix, and they have been reported to play an important role in tumor invasion, metastasis and angiogenesis. These enzymes are closely related to tumor malignancy and patient survival time. Recently, reversion-inducing cysteine-rich protein with Kazal motifs (RECK) gene was identified as an endogenous membrane-anchored MMP inhibitor. The down-regulation of RECK has been implicated in tumor progression. In this study, the expression levels of the RECK messenger ribonucleic acid (mRNA) in various spontaneously developed canine tumors were investigated by using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), and the correlation between RECK and clinicopathological factors, as well as MMP-9 expression were analyzed. The median age of 36 dogs investigated in this study was 9 years old (range, 1-15 years old). Quantitative RT-PCR could detect low levels of expression of RECK mRNA in the tumor samples. The expression levels of RECK mRNA in some tumor tissue samples were significantly lower than those in normal tissue samples. No significant associations of RECK with clinicopathological factors were observed. Using the Mann-Whitney U test, the expression level of the MMP-9 mRNA was observed to be significantly correlated to RECK expression (P < 0.05).
  • 腫瘍血管を標的とした光線力学療法の基礎的および臨床的検討
    大崎 智弘, 廉澤 剛, 星野 明佳, 星野 有希, 高木 哲, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 140回 181 -181 2005年08月 [査読無し][通常論文]
  • 星野有希, 奥村正裕, 大崎智弘, 高木哲, 藤永徹 北海道獣医師会雑誌 49 (8) 282 2005年08月01日 [査読無し][通常論文]
  • 大崎智弘, 廉沢剛, 星野有希, 高木哲, 奥村正裕, 藤永徹 北海道獣医師会雑誌 49 (8) 273 2005年08月01日 [査読無し][通常論文]
  • 高木 哲, 加藤 友香, 浅野 和之, 大崎 智弘, ボスナコフスキー ダルコ, 星野 有希, 奥村 正裕, 廉澤 剛, 藤永 徹 The journal of veterinary medical science 67 (8) 761 -7 2005年08月 [査読無し][通常論文]
     
    マトリクスメタロプロテイナーゼ(MMP), 特にMMP-2とMMP-9は腫瘍の浸潤, 転移に深く関わっているとされている.近年, これらのMMP活性を抑制する膜結合型タンパクであるRECKが発見され, ヒトの臨床例においてRECK発現量と腫瘍の悪性度には負の相関が認められている.本研究では, 犬の自然発生腫瘍症例におけるRECKmRNA発現量をリアルタイムPCR法にて定量し, 臨床的指標, MMP-9mRNAの相関について評価した.腫瘍組織とその由来する正常組織とのRECK発現量を比較したところ, いくつかの腫瘍組織で有意に発現が低くなっていた(p<0.05).年齢, 性別, リンパ節および肺転移の有無との有意差はそれぞれ認められなかった.MMP-9とRECKには有意な相関関係が認められた.腫瘍症例の予後とRECK発現量の相関関係を明らかにするにはより詳細な検討が必要であると思われた.
  • 海綿骨移植
    奥村 正裕 獣医麻酔外科学雑誌 36 (Suppl.1) 76 -77 2005年06月 [査読無し][通常論文]
  • 脊柱の外科的減圧が著効を示した脊髄空洞症の犬の1症例
    高木 哲, 廉澤 剛, 大崎 智弘, 星野 有希, 片山 豊裕, 久保 拓也, 横山 文香, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌(0018-3385) 49巻 (5号) Page4-9 -9 2005年05月 [査読無し][通常論文]
  • 高木 哲, 北村 剛規, 保坂 善真, 大崎 智弘, ダルコ・ボスナコフスキー, 廣澤 剛, 奥村 正裕, 藤永 徹 The Journal of Veterinary Medical Science 67 (4) 385 -391 2005年04月 [査読無し][通常論文]
     
    The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) gene is one of the endogenous matrix metalloproteinase (MMP) inhibitors. It was reported that decreased RECK expression closely correlated with tumor malignancy. We determined the cDNA sequence of the canine RECK gene. The cDNA sequence and deduced amino acid of canine RECK were 2,913 bases and 971 residues, respectively. The predicted amino acid sequence of the protein showed 95.5% and 91.9% homology with human and mouse RECK, respectively. RECK mRNA expression was analyzed in various canine tissues and tumor cell lines by quantitative RT-PCR. The highest RECK expression was detected in lung and testis. In comparison with the tissues, a remarkably low expression level was detected in tumor cell lines. In addition, the RECK gene was transfected in the canine transitional cell carcinoma, and its influence on cell proliferation. migration, and invasion was analyzed. The transfected RECK gene suppressed only canine tumor invasion. These results showed that RECK might play an important role in tumor malignancy in dogs as well as in other mammalians.
  • 高木 哲, 北村 剛規, 保坂 善真, 大崎 智弘, ボスナコフスキー ダルコ, 廉澤 剛, 奥村 正裕, 藤永 徹 The journal of veterinary medical science 67 (4) 385 -91 2005年04月 [査読無し][通常論文]
     
    RECKは近年発見された膜結合型内因性マトリクスメタロプロテイナーゼインヒビターであり, ヒトの臨床例においてその発現量と腫瘍の悪性度に負の相関が認められることが報告されている.本研究では犬のRECK遺伝子のクローニングを行った.その結果, cDNAは2,913塩基で, ヒトと95.5%, マウスと91.9%の相同性を示す971残基のアミノ酸から構成されることがわかった.様々な正常組織でのmRNAの発現量をリアルタイムPCR法にて定量した結果, 肺と精巣で高い発現が認められたが, 腫瘍細胞では極めて低い発現のみ認められた.免疫染色では精子, 平滑筋, 偽重層上皮などに強い染色性が認められ, 発現ペクターを用いた実験では腫瘍の浸潤能が抑制されていた.
  • 星野 有希, 廉澤 剛, 滝口 満喜, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌(0916-5908) 36 (1) 19 -22 2005年01月31日 [査読無し][通常論文]
  • 森 崇, 村上 正晃, 奥村 正裕, 廉澤 剛, 上出 利光, 藤永 徹 The Journal of Veterinary Medical Science 67 (1) 51 -56 2005年01月 [査読無し][通常論文]
     
    In order to analyze the detailed mechanisms responsible for macrophage activation by chitin derivatives, resident peritoneal macrophages were prepared and stimulated with chitin, chitosan and low-molecular weight chitosan. Our findings were as follows: (i) chitosan induced apoptosis of peritoneal macrophages, but this did not occur when chitin or water soluble low-molecular weight chitosan were used; (ii) chitosan treatment induced activation markers, such as the major histocompatibility complex (MHC) class I, class II, Fc receptors, transferrin receptor, mannose receptor, Fas, and macrophage inflammatory protein (MIP)-2, whereas chitin and low molecular weight soluble chitosan induced only the expression of MHC class I and 11 molecules; (iii) apoptosis induced by chitosan was mediated by the Fas signaling pathway, in response to phagocytosis via the mannose receptor. We conclude that since chitosan activates macrophages, this may be the mechanism by which it accelerates wound healing.
  • 森 崇, 村上 正晃, 奥村 正裕, 廉澤 剛, 上出 利光, 藤永 徹 The journal of veterinary medical science 67 (1) 51 -56 2005年01月 [査読無し][通常論文]
     
    キチン・キトサンは創傷治癒促進剤として広く用いられているが,その機構については不明な点が多い.今回マクロファージに対するキチン・キトサンの作用を解析する目的で,マウス腹腔マクロファージ(PM)をキチン,キトサンおよび低分子キトサンにて刺激した.その結果,キトサンはPMに対してFcレセプター,主要組織適合遺伝子複合体クラスI(H-2D^d)およびII(I-A^d),マンノースレセプター,トランスフェリンレセプター,およびFasの発現増強,IL-8スーパーファミリーに属するmacrophage inflammatoryprotein 2の産生誘導を起こすことが認められた.またキトサンはFas-Fasリガンドシステムによるアポトーシスを誘導し,これらの作用はマンノースレセプターを介していることが示唆された.キチンおよび低分子キトサンに関しては,I-A^dの発現増強以外は認められなかった.ごれらの結果から,キトサンはマンノースレセプターを介して貪食されることでマクロファージを活性化し,創傷治癒を促進しているのではないかと考えられた.
  • Takagi S, Kadosawa T, Ohsaki T, Hoshino Y, Okumura M, Fujinaga T. Hindbrain decompression in a dog with scoliosis associated with syringomyelia. J Am Vet Med Assoc. 2005 Apr 15;226(8):1359-63, 1347.*
    2005年 [査読無し][通常論文]
  • Takagi S, Kadosawa T, Ishiguro T, Ohsaki T, Okumura M, Fujinaga T. Urethral transitional cell carcinoma in a cat. J Small Anim Pract. 2005 Oct;46(10):504-6.*
    2005年 [査読無し][通常論文]
  • 獣医麻酔外科学雑誌(0916-5908) 35巻 (4号) Page89-94 2005年 [査読無し][通常論文]
  • Takagi S, Kitamura T, Hosaka Y, Ohsaki T, Bosnakovski D, Kadosawa T, Okumura M, Fujinaga T. Molecular cloning of canine membrane-anchored inhibitor of matrix metalloproteinase, RECK. J Vet Med Sci. 2005 Apr;67(4):385-91.*
    2005年 [査読無し][通常論文]
  • Bosnakovski D, Mizuno M, Kim G, Takagi S, Okumura M, Fujinaga T. Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells. Cell Tissue Res. 2005 Feb;319(2):243-53. Epub 2004 Nov 20.*
    2005年 [査読無し][通常論文]
  • Mori T, Murakami M, Okumura M, Kadosawa T, Uede T, Fujinaga T. Mechanism of macrophage activation by chitin derivatives. J Vet Med Sci. 2005 Jan;67(1):51-6.*
    2005年 [査読無し][通常論文]
  • Ishioka K, Okumura M, Sagawa M, Nakadomo F, Kimura K, Saito M. Computed tomographic assessment of body fat in beagles. Vet Radiol Ultrasound. 2005 Jan-Feb;46(1):49-53.*
    2005年 [査読無し][通常論文]
  • Takagi S, Kato Y, Asano K, Ohsaki T, Bosnakovski D, Hoshino Y, Okumura M, Kadosawa T, Fujinaga T. Matrix metalloproteinase inhibitor RECK expression in canine tumors. J Vet Med Sci. 2005 Aug;67(8):761-7.*
    2005年 [査読無し][通常論文]
  • 犬の関節疾患の診断と治療 診断へのアプローチ,治療方針の立て方
    奥村 正裕 J-VET 17 (11別冊付録) 26 -32 2004年11月 [査読無し][通常論文]
  • 肩甲骨を広範に切除した肩甲骨原発肉腫の犬1例
    奥村 正裕, 伊藤 慎一郎, 星野 有希, 大崎 智弘, 藤永 徹 獣医麻酔外科学雑誌 35 (Suppl.2) 82 -82 2004年10月 [査読無し][通常論文]
  • 高木 哲, 森 崇, 渡邊 一弘, 廉澤 剛, 落合 謙爾, 鳥越 慎吾, 北村 二郎, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 35 (4) 89 -94 2004年10月 [査読無し][通常論文]
  • 機能性食品(アガリクスタケ,タモギタケ,ヤマブシタケ,アラビノキシラン,サメ軟骨およびサメ抽出脂質)の摂取がマウスLM8 Dunn骨肉腫移植腫瘍の成長に及ぼす効果
    木戸 伸英, 高木 哲, 島田 健次郎, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 48 (9) 386 -390 2004年09月 [査読無し][通常論文]
  • 【骨癒合不全】 上腕骨の癒合不全
    奥村 正裕 SURGEON 8 (5) 34 -42 2004年09月 [査読無し][通常論文]
  • 渡邊 一弘, 廉澤 剛, 石黒 武人, 高木 哲, 落合 謙爾, 木村 享史, 奥村 正裕, 藤永 徹 The Journal of Veterinary Medical Science 66 (9) 1167 -1170 2004年09月 [査読無し][通常論文]
     
    Odontogenic cysts, which showed cystic radiolucency in the jaw bone by radiographic examination and computed tomography, were enucleated by operation in 3 dogs. One dog had a odontogenic keratocyst in the incisive bone of the right maxilla and another 2 cases revealed dentigerous cysts in the mandible. These cyst walls were enucleated or transpired by semiconductor laser. Afterwards, osteogenesis was confirmed at the defective part of jaw bone by extirpation of the cyst in all cases, and no recurrence has been noted in any cases. Odontogenic cyst is a disease which should be treated by surgical extirpation or transpiration.
  • 北海道の乳用牛の蹄病における乳期および牛舎環境の要因解析
    川崎 洋史, 鬼頭 宗寛, 木田 克弥, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 48 (8) 257 -257 2004年08月 [査読無し][通常論文]
  • 北海道の乳用牛の蹄病における代謝プロファイルテストを用いて行った栄養要因解析
    鬼頭 宗寛, 川崎 洋史, 木田 克弥, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 48 (8) 257 -257 2004年08月 [査読無し][通常論文]
  • 化学的胸膜癒着術を行った特発性乳糜胸の犬の1症例
    星野 有希, 廉澤 剛, 伊藤 慎一郎, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 48 (8) 300 -300 2004年08月 [査読無し][通常論文]
  • 肩甲骨部分切除術をおこなった犬の肩甲骨原発肉腫の1症例
    伊藤 慎一郎, 奥村 正裕, 櫻町 育夫, 星野 有希, 田之上 真一, 大崎 智弘, 藤永 徹 北海道獣医師会雑誌 48 (8) 310 -310 2004年08月 [査読無し][通常論文]
  • 外科的摘出が困難な上顎腫瘍に対して光線力学療法を行った犬の2症例
    大崎 智弘, 廉澤 剛, 星野 明佳, 高木 哲, 星野 有希, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 48 (8) 310 -310 2004年08月 [査読無し][通常論文]
  • 高木哲, 廉沢剛, 上野博史, 奥村正裕, 藤永徹 北海道獣医師会雑誌 48 (8) 361 -363 2004年08月01日 [査読無し][通常論文]
  • 腫瘍による肢端切断後にパッド移植を行ったイヌの1例
    高木 哲, 廉澤 剛, 上野 博史, 奥村 正裕, 藤永 徹 北獣会誌 48 (8) 125 -127 2004年08月 [査読無し][通常論文]
  • 大崎 智弘, 廉澤 剛, 松本 康宏, 高木 哲, 奥村 正裕, 藤永 徹 日本獣医師会雑誌 57 (6) 386 -390 2004年06月 [査読無し][通常論文]
     
    断脚あるいは抗癌剤による治療に同意が得られなかった橈骨遠位端の骨肉腫(SOS)および左肩甲部軟部組織のEOSの各1例に対し,腫瘍休眠療法を目的とした治療試験として血管新生阻害剤および分化誘導剤を全身的に使用した.両症例において,QOLが維持されるとともに腫瘍の進行が遅かったことから,これらの薬剤を用いた休眠療法は犬の骨肉腫に有効である可能性が示唆された
  • 北海道のホルスタイン健康牛における蹄病発生の実態調査
    川崎 洋史, 大崎 智弘, 鬼頭 宗寛, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 48 (5) 145 -150 2004年05月 [査読無し][通常論文]
  • 金 根亨, 奥村 正裕, Bosnakovski D, 石黒 武人, 廉澤 剛, 藤永 徹 The Journal of Veterinary Medical Science 66 (3) 263 -267 2004年03月 [査読無し][通常論文]
     
    To evaluate the effects of chondrocytes transplantation on the regeneration of cartilage by intraarticular injection or injection into blood clots at cartilage defects, eight full-thickness cartilage defects were created surgically on the articular surface of each femoral trochlea of two calves. Autologous chondrocytes were isolated individually from the cartilage pieces collected at the creation of defects. And isolated cells were cultured in monolayers for proliferation. Cells were injected into synovial fluid (Group 2, n=11) or into the blood clots at the cartilage defects (Group 3, n=5) of the left femoropatellar joint on weeks 2 and 3, respectively after the operation. The defects (Group 1, n=16) of right femoropatellar joint were left untreated in the control group. After 14 weeks, repaired tissues were evaluated based on gross and histological examinations. In Group 3, more repaired tissues and a better interface between the repaired tissue and host cartilage were observed compared with the results for Groups 1 and 2. Moreover, cartilaginous tissue were observed more in defects of Group 3 than in defects of other groups. In conclusion, the present study suggests that the injection of cells into the blood clot at a cartilage defect might be applicable for the regeneration of damaged cartilage.
  • 犬の移行上皮癌細胞株の樹立とその性状解析
    沖原 涼子, 廉澤 剛, 高木 哲, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 137回 147 -147 2004年03月 [査読無し][通常論文]
  • 機能性食品(アガリクスタケ、タモギタケ、ヤマブシタケ、アラビノキシラン、サメ軟骨および抽出エキス)の摂取がマウスLM8Dunn骨肉腫移植腫瘍の成長に及ぼす効果
    木戸 伸英, 高木 哲, 島田 健次郎, 廉澤 剛, 奥村 正裕, 藤永 徹 北獣会誌 48 386 -390 2004年03月 [査読無し][通常論文]
  • 上野 博史, 廉澤 剛, 落合 謙爾, 富岡 幸子, 奥村 正裕, 藤永 徹 日獣会誌 57 (2) 113 -115 2004年02月 [査読無し][通常論文]
  • 高木 哲, 森 崇, 渡邊 一弘, 廉澤 剛, 落合 謙爾, 鳥越 慎吾, 北村 二郎, 奥村 正裕, 藤永 徹 獣医麻酔外科誌 35 (4) 89 -94 2004年 [査読無し][通常論文]
  • Bosnakovski D, Mizuno M, Kim G, Ishiguro T, Okumura M, Iwanaga T, Kadosawa T, Fujinaga T. Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells in pellet cultural system. Exp Hematol. 32(5):502-9 (2004)*
    2004年 [査読無し][通常論文]
  • Kim G, Okumura M, Bosnakovski D, Ishiguro T, Kadosawa T, Fujinaga T. Regeneration of cartilage tissue by autologous chondrocytes transplantation for cartilage defects in a experimental bovine model.J Vet Med Sci. 66(3):263-7 (2004)*
    2004年 [査読無し][通常論文]
  • Kim G, Okumura M, Bosnakovski D, Ishiguro T, Kadosawa T, Fujinaga T. Regeneration of cartilage tissue by autologous chondrocytes transplantation for cartilage defects in a experimental bovine model. J Vet Med Sci. 66(3):263-7 (2004)*
    2004年 [査読無し][通常論文]
  • 主要症状を基礎にした猫の臨床<前出吉光>
    デーリィマン社 2004年 [査読無し][通常論文]
  • (翻訳)動物の痛みをどのようにして認識するか?小動物における疼痛評価(Robertson, S., A.著)
    2004年 [査読無し][通常論文]
  • 小動物の関節疾患治療における消炎・鎮痛の意義 関節疾患の診断・治療と非ステロイド性抗炎症薬(NSAIDs)使用の実際
    奥村 正裕 動物臨床医学会年次大会プロシーディング 24回 (3) 121 -125 2003年11月 [査読無し][通常論文]
  • 【犬の骨軟骨症】 離断性骨軟骨症の発生機序と病態
    奥村 正裕 SURGEON 7 (5) 6 -13 2003年09月 [査読無し][通常論文]
  • 関節疾患の診断的アプローチ:画像診断と関節液の分析
    奥村 正裕 獣医麻酔外科学雑誌 34 (Suppl.2) 71 -74 2003年09月 [査読無し][通常論文]
  • 紐状異物による十二指腸の線状穿孔に対して腸管内側から修復を行った犬の一例
    星野 有希, 廉澤 剛, 滝口 満喜, 松久 祐貴, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 34 (Suppl.2) 99 -100 2003年09月 [査読無し][通常論文]
  • 腫瘍に対して新生血管閉塞効果を応用した光線力学療法を行った犬の4症例
    大崎 智弘, 廉澤 剛, 星野 明佳, 高木 哲, 田中 瑞穂, 田澤 由紀, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 34 (Suppl.2) 104 -104 2003年09月 [査読無し][通常論文]
  • 患肢温存療法として肩甲骨全摘出術を行った骨軟骨腫症の猫1例
    鬼頭 宗寛, 廉澤 剛, 高木 哲, 星野 有希, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 34 (Suppl.2) 105 -106 2003年09月 [査読無し][通常論文]
  • 増生滑膜の焼灼,関節内洗浄及びヒアルロン酸関節内注入により治癒した感染性関節炎の犬1例
    横山 文香, 奥村 正裕, 大崎 智弘, 星野 明佳, 廉澤 剛, 藤永 徹 獣医麻酔外科学雑誌 34 (Suppl.2) 107 -107 2003年09月 [査読無し][通常論文]
  • 渡邊 一弘, 菊池 正浩, 奥村 正裕, 廉澤 剛, 藤永 徹 The Journal of Veterinary Medical Science 65 (9) 1007 -1010 2003年09月 [査読無し][通常論文]
     
    Enamel matrix protein (EMP) was applied for regeneration of periodontal tissue in 2 dogs with spontaneous periodontal disease. Case I had bony resorption around the root and root apex of the maxillary fourth premolars. Case 2 had vertical resorption of bone between the mandibular first and second molars. A flap was formed in the buccal gingiva, and EMP was applied onto the surface of the exposed root. One or 4 months postoperatively, increased bone level and clinical attachment were recognized. EMP was therefore suggested to be effective to induce regeneration of periodontal tissues in the cases with periodontal disease.
  • 牛軟骨細胞の関節内および欠損部血塊内移植による損傷軟骨の実験的再生療法の検討
    金 根亨, 奥村 正裕, Bosnakovski Darko, 石黒 武人, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 136回 212 -212 2003年09月 [査読無し][通常論文]
  • ペレット培養におけるウシ骨髄-間葉幹細胞(MSC)の軟骨形成可能性(Chondrogenic potential of bovine bone marrow-mesenchymal stem cells (MSC) in pellet culture)
    Bosnakovski Darko, 水野 守道, 金 根亨, 石黒 武人, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 136回 212 -212 2003年09月 [査読無し][通常論文]
  • 犬内因性MMP阻害因子RECK遺伝子のクローニングとその発現
    高木 哲, 北村 剛規, 廉澤 剛, 大崎 智宏, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 136回 216 -216 2003年09月 [査読無し][通常論文]
  • 乳牛における廃用の要因としての蹄病の意義に関する一考察
    川崎 洋史, 鬼頭 宗寛, 大崎 智弘, 中禮 敏彦, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 47 (8) 283 -283 2003年08月 [査読無し][通常論文]
  • 鼻平面の偏平上皮癌に対して腫瘍血管を標的とした光線力学療法を実施し完全奏効を得た犬の1症例
    大崎 智弘, 廉澤 剛, 田中 瑞穂, 星野 明佳, 藤田 徹, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 47 (8) 292 -292 2003年08月 [査読無し][通常論文]
  • Ilizarov創外固定法を適応した前腕骨遠位骨折癒合不全のポメラニアンの1症例
    沖原 涼子, 奥村 正裕, 大崎 智弘, 木戸 伸英, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 47 (8) 306 -306 2003年08月 [査読無し][通常論文]
  • 粥状動脈硬化症を発症した甲状腺機能低下症の犬の1症例
    木戸 伸英, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 47 (8) 309 -309 2003年08月 [査読無し][通常論文]
  • 慢性肩甲上腕関節跛行の犬3例における関節鏡検査の活用
    奥村 正裕, 金 根亨, Bosnakovski Darko, 横山 文香, 大崎 智弘, 藤永 徹 獣医麻酔外科学雑誌 34 (Suppl.1) 243 -243 2003年06月 [査読無し][通常論文]
  • 金 根亨, 奥村 正裕, 石黒 武人, 廉澤 剛, 藤永 徹 The Journal of Veterinary Medical Science 65 (3) 427 -430 2003年03月 [査読無し][通常論文]
     
    Attachment and migration of bovine chondrocytes cultured in vitro were significantly suppressed by the addition of interleukin (IL)-1alpha at the concentration of 1 ng/ml or more (p<0.05). The application of hyaluronic acid (HA) at the concentration of 10 mug/ml or more significantly recovered the attachment of chondrocytes (p<0.05) and the application of HA at 100 mug/ml concentration recovered the migration of chondrocytes suppressed by IL-1alpha. These results suggest that the application of HA for inflammatory arthropathies or chondrocyte transplantation might be helpful to preserve the properties of chondrocytes and its extracellular matrix against inflammatory conditions.
  • 犬関節疾患の病態解析における関節液中軟骨同化マーカーの有用性
    横山 文香, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 135回 189 -189 2003年03月 [査読無し][通常論文]
  • 犬の分離肺換気麻酔における持続的陽圧換気,持続的気道内陽圧ならびに間歇的強制換気の効果
    河村 貴央, 沖原 涼子, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 135回 190 -190 2003年03月 [査読無し][通常論文]
  • G Kim, M Okumura, T Ishiguro, T Kadosawa, T Fujinaga JOURNAL OF VETERINARY MEDICAL SCIENCE 65 (3) 427 -430 2003年03月 [査読無し][通常論文]
     
    Attachment and migration of bovine chondrocytes cultured in vitro were significantly suppressed by the addition of interleukin (IL)-1alpha at the concentration of 1 ng/ml or more (p<0.05). The application of hyaluronic acid (HA) at the concentration of 10 mug/ml or more significantly recovered the attachment of chondrocytes (p<0.05) and the application of HA at 100 mug/ml concentration recovered the migration of chondrocytes suppressed by IL-1alpha. These results suggest that the application of HA for inflammatory arthropathies or chondrocyte transplantation might be helpful to preserve the properties of chondrocytes and its extracellular matrix against inflammatory conditions.
  • Watanabe K, Kikuchi M, Okumura M, Kadosawa T, Fujinaga T. Efficacy of enamel matrix protein applied to spontaneous periodontal disease in two dogs. J. Vet. Med. Sci. 65(9):1007-10.*
    2003年 [査読無し][通常論文]
  • Ishiguro T, Kadosawa T, Takagi S, Kim G, Ohsaki T, Bosnakovski D, Okumura M, Fujinaga T. Relationship of disease progression and plasma histamine concentrations in 11 dogs with mast cell tumors. J. Vet. Intern. Med. 17(2):194-8.*
    2003年 [査読無し][通常論文]
  • Kim G, Okumura M, Bosnakovski D, Ishiguro T, Park CH, Kadosawa T, Fujinaga T. Effects of ascorbic acid on proliferation and biological properties of bovine chondrocytes in alginate beads. Jpn J. Vet. Res. 51(2):83-94.*
    2003年 [査読無し][通常論文]
  • Kim G, Okumura M, Bosnakovski D, Ishiguro T, Kadosawa T, Fujinaga T. Biological properties of allogenic articular chondrocytes on the surface of bovine cartilage explants in vitro. J. Vet. Med. A 50(8):418-23.*
    2003年 [査読無し][通常論文]
  • 肥満細胞腫に対する放射線治療後に急性低ナトリウム血症を生じた犬の1例
    石黒 武人, 安孫子 美奈, 金 根亨, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 46 (11) 381 -384 2002年11月 [査読無し][通常論文]
  • イヌの肥満に対する魚油給餌の効果とUCP発現
    石岡 克己, ソマリン・M.M, 奥村 正裕, 佐川 真由美, 柴田 治樹, 本庄 勉, 木村 和弘, 斉藤 昌之 獣医生化学 39 (2) 32 -32 2002年10月 [査読無し][通常論文]
  • 犬の歯周疾患にエナメルマトリックスタンパクを応用した歯周組織再生治療
    渡邊 一弘, 奥村 正裕, 廉澤 剛, 藤永 徹 SURGEON 6 (5) 64 -72 2002年09月 [査読無し][通常論文]
  • 放射線治療後に急性低ナトリウム血症を生じた肥満細胞腫の犬の1例
    石黒 武人, 安孫子 美奈, 金 根亨, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 46 (8) 273 -273 2002年08月 [査読無し][通常論文]
  • 血管新生阻害剤及び分化誘導剤による治療試験を行った骨格外骨肉腫の犬の1症例
    大崎 智弘, 廉澤 剛, 高木 哲, 橋本 武志, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 46 (8) 274 -274 2002年08月 [査読無し][通常論文]
  • 犬の脊髄疾患15症例における脊髄造影検査所見とコンピュータ断層撮影検査所見との比較
    細谷 謙次, 廉澤 剛, 奥村 正裕, 高木 哲, 大崎 智弘, 田澤 由紀, 田中 瑞穂, 藤永 徹 北海道獣医師会雑誌 46 (8) 284 -284 2002年08月 [査読無し][通常論文]
  • 含歯性歯原性襄胞を認めたイヌの2症例
    高木 哲, 廉澤 剛, 渡邊 一弘, 石黒 武人, 藤田 修, 田之上 真一, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 46 (8) 285 -285 2002年08月 [査読無し][通常論文]
  • 尺骨遠位成長板早期閉鎖に起因する成長性変形をイリザロフ創外固定法により矯正した犬の1例
    河村 貴央, 奥村 正裕, 高木 哲, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 46 (8) 294 -294 2002年08月 [査読無し][通常論文]
  • 選択的チロシンキナーゼ阻害剤の犬肥満細胞腫細胞に対する増殖抑制効果
    石黒 武人, 廉澤 剛, 金 根亨, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 134回 158 -158 2002年08月 [査読無し][通常論文]
  • 牛培養軟骨細胞の同種軟骨組織への接着性及び増殖性
    金 根亨, 奥村 正裕, Darko Bosnakovski, 石黒 武人, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 134回 253 -253 2002年08月 [査読無し][通常論文]
  • 肥満細胞腫罹患犬11頭の病歴と血漿ヒスタミン濃度との関係
    石黒 武人, 廉澤 剛, 金 根亨, 高木 哲, 大崎 智弘, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 46 (7) 188 -192 2002年07月 [査読無し][通常論文]
  • 下顎骨に強い骨増生を認めた扁平上皮癌の雑種猫の2例
    高木 哲, 廉澤 剛, 落合 謙爾, 鳥越 慎吾, 北村 二郎, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 33 (Suppl.1) 128 -128 2002年06月 [査読無し][通常論文]
  • 前十字靱帯不全断裂が疑われたイヌにおける関節鏡所見
    奥村 正裕, 大町 麻子, 横山 文香, 高木 哲, 廉澤 剛, 藤永 徹 獣医麻酔外科学雑誌 33 (Suppl.1) 145 -145 2002年06月 [査読無し][通常論文]
  • ゴールデンレトリバーの歯肉に生じた組織病理学的悪性所見の乏しい線維肉腫
    廉澤 剛, 高木 哲, 安孫子 美奈, 大崎 智弘, 奥村 正裕, 松田 一哉, 磯村 洋, 藤永 徹 獣医麻酔外科学雑誌 33 (Suppl.1) 121 -121 2002年06月 [査読無し][通常論文]
  • 腫瘍の光線力学療法における新規糖連結ポルフィリン化合物および糖連結クロリン化合物のスクリーニング
    細谷 謙次, 加藤 淳, 廣原 志保, 矢野 重信, 佐藤 勝彦, 奥村 正裕, 廉澤 剛, 藤永 徹 日本レーザー医学会誌 23 (2) 65 -65 2002年06月 [査読無し][通常論文]
  • イヌの肥満に対する魚油給餌の効果とUCP発現
    石岡 克己, モハマド・ソリマン, 奥村 正裕, 佐川 真由美, 柴田 治樹, 本庄 勉, 北村 浩, 木村 和弘, 斉藤 昌之 獣医生化学 39 (1) 31 -38 2002年04月 [査読無し][通常論文]
  • 北海道のホルスタイン牛における蹄病発生の実態調査
    大崎 智弘, 中禮 敏彦, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 133回 148 -148 2002年03月 [査読無し][通常論文]
  • 腫瘍の光線力学療法における新規糖連結ポルフィリン化合物及び糖連結クロリン化合物の有用性
    加藤 淳, 奥村 正裕, 廉澤 剛, 佐藤 勝彦, 廣原 志保, 矢野 重信, 藤永 徹 日本獣医学会学術集会講演要旨集 133回 154 -154 2002年03月 [査読無し][通常論文]
  • 犬の片肺換気麻酔における持続的陽圧換気法の効果
    安孫子 美奈, 河村 貴央, 秀嶋 綾, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 133回 159 -159 2002年03月 [査読無し][通常論文]
  • ヒトインターロイキン-1α刺激下ウシ軟骨細胞の接着及び移動能に対するヒアルロン酸の効果
    金 根亨, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 133回 159 -159 2002年03月 [査読無し][通常論文]
  • イヌの肥満に対する魚油給餌の効果とUCP発現
    石岡 克己, 佐川 真由美, 奥村 正裕, 柴田 治樹, 本庄 勉, 北村 浩, 木村 和弘, 斉藤 昌之 日本獣医学会学術集会講演要旨集 133回 171 -171 2002年03月 [査読無し][通常論文]
  • Okumura M, Kim GH, Tagami M, Haramaki S, Fujinaga T, Serum keratan sulphate as a cartilage metabolic marker in horses: the effect of exercise. J Vet Med A Physiol Pathol Clin Med. 2002;49(4):195-7.*
    2002年 [査読無し][通常論文]
  • Ueno H, Kadosawa T, Isomura H, Okada Y, Ochiai K, Umemura T, Okumura M, Fujinaga T, Perianal rhabdomyosarcoma in a dog. J Small Anim Pract. 2002;43(5):217-20.*
    2002年 [査読無し][通常論文]
  • 石黒 武人, 廉澤 剛, 森 浩志, 高木 哲, 奥村 正裕, 藤永 徹 The Journal of Veterinary Medical Science 63 (9) 1031 -1034 2001年09月 [査読無し][通常論文]
     
    A new cell line (CoMS) was established from a 3-year-old male mongrel dog with mast cell tumor of the oral mucosa. coma. cells grow in suspension with a doubling time of 27.0 +/- 0.7 hr, The cytoplasmic granules were formalin-sensitive, showed diverse appearances in their ultrastructural findings and contained heparin proteoglycan and neutral protease chymase. Calcium ionophore A23187, substance P and concanavalin A caused significant histamine release from CoMS cells, while compound 48/80 failed to release histamine. This cell line will make an available source for studies on canine mast cell tumors.
  • 犬における脊髄空洞症を伴うキアリ奇形に外科的治療を適応した一症例
    加藤 淳, 廉澤 剛, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 45 (8) 299 -299 2001年08月 [査読無し][通常論文]
  • ゴールデンレトリバー犬の口腔内に発生した線維肉腫の四症例
    安孫子 美奈, 廉澤 剛, 附田 由紀, 石田 修, 落合 謙爾, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 45 (8) 292 -292 2001年08月 [査読無し][通常論文]
  • 犬の骨肉腫に姑息的放射線療法,血管新生阻害剤及び分化誘導剤を用いて患肢温存を試みた一症例
    大崎 智弘, 松本 康宏, 廉澤 剛, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 45 (8) 297 -297 2001年08月 [査読無し][通常論文]
  • 牛の蹄病に関する研究 ホルスタイン種の肥育牛と経産牛における蹄底角質及び蹄底真皮の病態調査
    大崎 智弘, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 45 (8) 247 -247 2001年08月 [査読無し][通常論文]
  • 病態の把握が困難であった犬の二次性変形性膝関節症の関節鏡を用いた診断
    奥村 正裕, 金 根亨, 塚根 悦子, 田澤 由紀, 田中 瑞穂, 石黒 武人, 高木 哲, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 45 (8) 278 -278 2001年08月 [査読無し][通常論文]
  • 脾静脈を経由した門脈体循環シャントにアメロイド・コンストリクターを用いた犬の二症例
    塚根 悦子, 廉澤 剛, 奥村 正裕, 藤永 徹, 新井田 潤, 村上 喜久代 北海道獣医師会雑誌 45 (8) 280 -280 2001年08月 [査読無し][通常論文]
  • 渡邊 一弘, 菊池 正浩, 奥村 正裕, 廉澤 剛, 藤永 徹 The Journal of Veterinary Medical Science 63 (8) 889 -894 2001年08月 [査読無し][通常論文]
     
    Adult dogs have a complex apical delta structure in all root apexes of teeth. This complex structure may affect the formation of apical lesions in the teeth such as apical abscesses. The purpose of this study was to evaluate the efficacy of enamel matrix protein (EMP) which was used for periodontal regeneration therapy after an experimental apicoectomy for an assumed apical lesions of the teeth in dogs. The maxillar canine roots and maxillar fourth premolar buccal mesial roots in five beagles were experimentally apicoectomized under general inhalation anesthesia. After the root apex was exposed and excised, EMP was applied on the surface of the exposed dentin. After 12 weeks, dogs were euthanized, and, the experimental teeth together with the surrounding soft and hard periodontal tissues were collected for histological evaluation under a light microscope. In the EMP group, the size of the defect where the root apex was removed was smaller than that of the control group. New cementum was dominantly achieved in the EMP group compared to the control group. Furthermore, new collagen fibers that bridged area between the new cementum and new alveolar bone were detected only in the EMP group. The present results demonstrated marked apical periodontal regeneration after apicoectomy in the EMP group. These results, therefore, suggest that the application of EMP can effectively induce the regeneration of periodontal structures in apicoectomized dogs.
  • 骨・関節疾患の画像診断と外科療法 後肢疾患の画像診断
    奥村 正裕 獣医麻酔外科学雑誌 32 (Suppl.1) 38 -42 2001年07月 [査読無し][通常論文]
  • 膀胱全摘及び尿管-尿道吻合により治療した膀胱癌のイヌの1症例
    廉澤 剛, 落合 謙爾, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 32 (Suppl.1) 174 -175 2001年07月 [査読無し][通常論文]
  • 渡邊 一弘, 菊池 正浩, Barroga E.F, 奥村 正裕, 廉澤 剛, 藤永 徹 The Journal of Veterinary Medical Science 63 (7) 789 -795 2001年07月 [査読無し][通常論文]
     
    To determine the process of formation of apical delta, a histological study on the permanent teeth was carried out in dogs. A litter of 7 clinically healthy beagle dogs and 33 adult dogs (4- to 15- year-old) of 12 breeds with periodontal disease were used for the experiments. Teeth extracted from 6-,7-,8- and 9-month-old beagles were sectioned and stained with HE solution. Tooth roots obtained from adult dogs with periodontal disease were ground. Each tooth was classified into the following root types under a light microscope: Type I (no apical delta = no apical closure), II (few apical delta), IIIA (low apical delta) and IIIB (high apical delta). In the 6-month-old beagles, more than half the tooth roots were classified as type I. In the 7-month-old beagles, type IIIB apical delta was the most predominant and types I, II and IIIA apical delta were occassionally seen. Apical closure and delta were observed in all beagles at 8 months of age histologically. In the 8- and 9-month-old beagles, all root apexes observed were type IIIB. Most of the 314 tooth roots extracted from 33 adult dogs were type IIIB, but a few were type IIIA.
  • 犬と猫の腫瘍症例における「アガリクス茸」及び「アガリクス茸+サメ軟骨」の臨床学的効果
    廉澤 剛, 奥村 正裕, 島田 健次郎, 藤永 徹 小動物臨床 20 (2) 101 -109 2001年03月 [査読無し][通常論文]
  • 浅野 和之, 増田 桂子, 奥村 正裕, 廉澤 剛, 藤永 徹 The Journal of Veterinary Medical Science 63 (3) 243 -250 2001年03月 [査読無し][通常論文]
     
    Association between exogenous atrial natriuretic peptide (ANP) and hemodynamic changes was ascertained in 3 dogs with overt congestive heart failure (CHF(+)) and 3 dogs without congestive heart failure (CHF(-)) caused by experimental mitral regurgitation (MR). The hemodynamic measurements were recorded in all dogs during and after 1 hr infusion of ANP at the rate of 0.1 (low dose), 0.5 (medium dose) and 1.0 (high dose) mug/kg/min, respectively. Heart rate, mean arterial pressure, pulmonary capillary wedge pressure (PCWP) and systemic vascular resistance decreased significantly during and after ANP infusion even with low dose in the CHF(+). Stroke volume, stroke volume index and cardiac output in the CHF(+) during and after ANP infusion showed an increasing trend as compared with the CHF(-). Double product, an indicator of myocardial oxygen consumption, significantly decreased during and after ANP administration at all doses in the CHF(+). These findings indicate that even at low dose, exogenous ANP improves cardiac performance and reduces myocardial oxygen consumption in the CHF(+), and suggest that ANP has beneficial effects in the treatment of dogs with overt congestive heart failure resulting from MR.
  • イヌの片肺換気麻酔における換気条件の相違が循環・呼吸動態に及ぼす影響
    秀嶋 綾, 安孫子 美奈, 神谷 和宏, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 131回 135 -135 2001年03月 [査読無し][通常論文]
  • イヌの早期変形性関節症モデルにおける画像診断所見と関節マーカーの有用性
    大町 麻子, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 131回 139 -139 2001年03月 [査読無し][通常論文]
  • コンピューター断層撮影法(CT)を用いたイヌの肥満・体脂肪評価
    石岡 克己, 奥村 正裕, 佐川 真由美, 中塘 二三生, 北村 浩, 木村 和弘, 斉藤 昌之 日本獣医学会学術集会講演要旨集 131回 150 -150 2001年03月 [査読無し][通常論文]
  • Mori,T.,Kadosawa,T.,Okada,Y.,Ochiai,K.,Umemura,T.,Tanoue,S.,Okumura,M.,Fujinaga,T.:"Acute respiratory failure caused by leukaemic infiltration of the lung of a dog.",Journal of Small Animal Practice,42:349-51(2001)*
    2001年 [査読無し][通常論文]
  • Ishiguro,T.,Kadosawa,T.,Mori,K.,Takagi,S.,Okumura,M.,Fujinaga,T.:"Establishment and characterization of a new canine mast cell tumor cell line.",Journal of Veterinary Medical Science,63:1031-1034(2001)*
    2001年 [査読無し][通常論文]
  • Kitamura,H.,Okumura,M.,Sato,F.,Kimoto,K.,Kohama,M.,Hashimoto,Y.,Tagami,M.,Iwanaga,T.:"Increased concentrations of protein gene product 9.5 in the synovial fluid from horses with osteoarthritis.",Japanese Journal of Veterinary Research,49:115-23(2001)*
    2001年 [査読無し][通常論文]
  • Watanabe,K.,Kikuchi,M.,Okumura,M.,Kadosawa,T.,Fujinaga,T.:"Efficacy of enamel matrix proteins on apical periodontal regeneration after experimental apicoectomy in dogs.",Journal of Veterinary Medical Science,63:889-94(2001)*
    2001年 [査読無し][通常論文]
  • Watanabe,K.,Kikuchi,M.,Barroga,E.F.,Okumura,M.,Kadosawa,T.,Fujinaga,T.:"The formation of apical delta of the permanent teeth in dogs.".Journal of Veterinary Medical Science,63:789-95(2001)*
    2001年 [査読無し][通常論文]
  • Ueno,H.,Murakami,M.,Okumura,M.,Kadosawa,T.,Uede,T.,Fujinaga,T.:"Chitosan accelerates the production of osteopontin from polymorphonuclear leukocytes.",Biomaterials,22:1667-73(2001)*
    2001年 [査読無し][通常論文]
  • 「犬と猫の腫瘍症例における「アガリクス茸」および「アガリクス茸+サメ軟骨」の臨床学的効果」
    『小動物臨床』 20 101 -109 2001年 [査読無し][通常論文]
  • Asano,K.,Masuda,K.,Okumura,M.,Kadosawa,T.,Fujinaga,T.:"Association between exogenous atrial natriuretic peptide and hemodynamics in dogs with congestive heart failure produced by experimental mitral regurgitation.",Jouranl of Veterinary Medical Science・・・
    2001年 [査読無し][通常論文]
     
    Asano,K.,Masuda,K.,Okumura,M.,Kadosawa,T.,Fujinaga,T.:"Association between exogenous atrial natriuretic peptide and hemodynamics in dogs with congestive heart failure produced by experimental mitral regurgitation.",Jouranl of Veterinary Medical Science,63:243-50(2001)*
  • 頸部に骨格外骨肉腫を発症したイヌの一症例
    木内 武雄, 廉澤 剛, 渡邊 一弘, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 44 (11) 20 -22 2000年11月 [査読無し][通常論文]
  • 犬と猫の腫瘍疾患に対するアガリクス茸およびサメ軟骨投与の臨床学的効果
    廉澤 剛, 奥村 正裕, 島田 健次郎, 藤永 徹 動物臨床医学会年次大会プロシーディング 21回 (3) 16 -22 2000年11月 [査読無し][通常論文]
  • 犬の根尖部apical deltaの形成と歯周疾患に対するエナメルマトリックスタンパクの歯周組織再生効果
    渡邊 一弘, 奥村 正裕, 廉澤 剛, 藤永 徹 動物臨床医学会年次大会プロシーディング 21回 (3) 44 -54 2000年11月 [査読無し][通常論文]
  • 「緩徐な進行を示した腰椎原発の骨肉腫のイヌの1例」
    高木 哲, 廉澤 剛, 佐藤 和男, 落合 謙爾, 磯村 洋, 上野 博史, 梅村 孝司, 奥村 正裕, 藤永 徹 『北獣会誌』 44 (9) 323 -325 2000年09月 [査読無し][通常論文]
  • 腫瘍による肢端切断後にパッド移植を行ったイヌの1例
    高木 哲, 廉澤 剛, 上野 博史, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 44 (7) 246 -246 2000年07月 [査読無し][通常論文]
  • 眼窩下動脈を温存し上顎骨部分切除術を行った口腔内線維肉腫のイヌの1症例
    秀嶋 綾, 廉澤 剛, 佐藤 真佐岐, 落合 謙爾, 梅村 孝司, 渡邊 一弘, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 44 (7) 245 -245 2000年07月 [査読無し][通常論文]
  • イヌ尺骨内側鈎状突起離断症(FMCP)の治療における関節鏡下手術の有用性
    奥村 正裕, 金 根亨, 石黒 武人, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 44 (7) 233 -233 2000年07月 [査読無し][通常論文]
  • 馬尾症候群と診断されたイヌ2症例における静脈内造影X線CT検査の有用性
    大町 麻子, 奥村 正裕, 藤田 徹, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 44 (7) 250 -250 2000年07月 [査読無し][通常論文]
  • 膀胱腫瘍により膀胱全摘出術を行ったイヌの雄・雌の各1症例
    松本 康宏, 廉澤 剛, 寺田 由利子, 森谷 康, 澤田 倍美, 落合 謙爾, 梅村 孝司, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 44 (7) 247 -247 2000年07月 [査読無し][通常論文]
  • 両側上腕関節窩靱帯の再建術を行った肩甲上腕関節不安定症のイヌの1例
    堤 奈々恵, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 44 (5) 129 -131 2000年05月 [査読無し][通常論文]
  • 渡邊 一弘, 廉澤 剛, 宮田 佳代子, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 30 (1〜2) 35 -41 2000年04月 [査読無し][通常論文]
  • 神谷 和宏, 秀嶋 綾, 奥村 正裕, 廉澤 剛, 藤永 徹 獣医麻酔外科学雑誌 31 (1〜2) 15 -24 2000年04月 [査読無し][通常論文]
  • 奥村 正裕, 田上 正明, 藤永 徹 The Journal of Veterinary Medical Science 62 (3) 281 -285 2000年03月 [査読無し][通常論文]
  • 神経症状を呈するネコにおけるボルナ病ウイルス感染の診断と病体の調査
    西野 佳以, 吉田 倫子, 田所 真弓, 栗田 吾郎, 辻本 元, 奥村 正裕, 木村 享史, 水谷 哲也 日本獣医学会学術集会講演要旨集 129回 81 -81 2000年03月 [査読無し][通常論文]
  • キトサンの腫瘍増殖及び転移に及ぼす効果
    堤 奈々恵, 上野 博史, 廉澤 剛, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 129回 139 -139 2000年03月 [査読無し][通常論文]
  • 好中球はオステオポンチン産生能を有する
    上野 博史, 村上 正晃, 奥村 正裕, 廉沢 剛, 上出 利光, 藤永 徹 日本獣医学会学術集会講演要旨集 129回 140 -140 2000年03月 [査読無し][通常論文]
  • ネコの乳癌に対する分化誘導療法の有用性に関する基礎的研究
    高木 哲, 廉澤 剛, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 129回 141 -141 2000年03月 [査読無し][通常論文]
  • イヌ骨肉腫由来ヌードマウス高肺転移モデルを用いた分化誘導療法の検討
    廉澤 剛, エドワード・バローガ, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 129回 143 -143 2000年03月 [査読無し][通常論文]
  • M Okumura, M Tagami, T Fujinaga JOURNAL OF VETERINARY MEDICAL SCIENCE 62 (3) 281 -285 2000年03月 [査読無し][通常論文]
     
    The role of keratan sulphate (KS) as a marker of cartilage metabolism was evaluated by using an in vitro model of equine articular cartilage. Articular cartilage was harvested from clinically healthy 6-month-old foals (n=3). Chondrocytes were centrifuged and cultured as pellets, Chondrocyte pellets were stimulated by insulin-like growth factor (IGF)-I alpha or interleukin (IL)-1 alpha for 2 weeks. The sulfated glycosaminoglycans (GAG) and antigenic KS concentrations in the culture media were measured by a 1,9-dimethyl-methylene blue (DMMB) colorimetric assay and an inhibition ELISA using a 1/14/16H9 antibody, respectively. Concentration of GAG was significantly increased in the media of pellets stimulated by both IGF-I alpha and IL-1 alpha. Antigenic KS concentration was significantly increased in those stimulated by IL-1 alpha, while no significant change was found in those stimulated by IGF-I alpha. A high correlation between GAG and antigenic KS concentrations was found in the media of pellets stimulated by IL-1 alpha (r=0.87), but not in those stimulated by IGF-I alpha (r=0.43). The results suggest that the concentration of antigenic KS reacting to 1/14/16H9 mirrors the GAG concentration during the stage of cartilage catabolism, but not during the cartilage anabolic stage. The concentration of antigenic KS reacting to 1/14/16H9 antibody in biological fluids could therefore be a useful marker to further understand principally the catabolic and slightly the anabolic process of articular cartilage metabolism.
  • 肘異形成を伴う習慣性肘関節脱臼と側副靱帯再建及び関節固定ピンを適用した犬の1例
    奥村 正裕, 堤 奈々恵, 志尾 大一, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 44 (2) 13 -15 2000年02月 [査読無し][通常論文]
  • イヌの尺骨内側鈎状突起離断症(FMCP)の治療における関節鏡下手術の有用性
    北獣会誌 44 233 2000年 [査読無し][通常論文]
  • 眼窩下動脈を温存した上腕骨部分切除術を行った口腔内線維肉腫のイヌの1例
    北獣会誌 44 245 2000年 [査読無し][通常論文]
  • 馬尾症候群と診断されたイヌ2例における静脈内造影X線CT検査の有用性
    北獣会誌 44 250 -2000 2000年 [査読無し][通常論文]
  • 膀胱腫瘍により膀胱全摘出術を行ったイヌの雄・雌の各1例
    北獣会誌 44 247 2000年 [査読無し][通常論文]
  • 腫瘍による肢端切除後にパッド移植術を行ったイヌの1例
    北獣会誌 44 246 2000年 [査読無し][通常論文]
  • 「頚部に骨外性骨肉腫を発症したイヌの一症例.」
    『北獣会誌』 44 394 -396 2000年 [査読無し][通常論文]
  • Okumura M, Watanabe K, Kadosawa T, Fujinaga T:"Surgical salvage from comminuted metatarsal fracture using a weight-bearing pin-putty apparatus in a dog." Aust. Vet. J. 78, 95-98(2000).
    2000年 [査読無し][通常論文]
  • 「肘異形成を伴う習慣性肘関節脱臼と側副靭帯再建および関節固定ピンを適用した犬の一例.」
    『北獣会誌』 44 45 -15 2000年 [査読無し][通常論文]
  • 「両側上腕関節窩靭帯の再建術を行った肩上腕関節不安定症のイヌの1例.」
    『北獣会誌』 44 129 -131 2000年 [査読無し][通常論文]
  • Barroga EF, Kadosawa T, Okumura M, Fujinaga T:"Inhibitory effects of 22-oxa-calcitoriol and all-trans retinoic acid on the growth of a canine osteosarcoma derived cell-line in vivo and its pulumonary metastasis in vivo." Res. Vet. Sci. 68:79-87(2000).
    2000年 [査読無し][通常論文]
  • Barroga EF, Kadosawa T, Okumura M, Fujinaga T:"Influence of vitamin D and retinoids on the induction of functional differenciation in vitro of canine osteosarcoma clonal cells." Vet. J. 159:186-193(2000).
    2000年 [査読無し][通常論文]
  • Okumura M, Fujinaga T:"Evaluation of the role of keratan sulphate as a molecular marker to monitor cartilage metabolism in horses." J. Vet. Med. A. 47:99-105(2000).
    2000年 [査読無し][通常論文]
  • 神谷 和宏, 秀嶋 綾, 奥村 正裕, 廉澤 剛, 藤永 徹 『獣医麻酔外科誌』 31 (1/2) 15 -24 2000年 [査読無し][通常論文]
  • 「老齢動物の慢性関節痛.変形性関節症と関節痛の診断と治療.」
    『S.A. Medicine』 2 14 -24 2000年 [査読無し][通常論文]
  • E Barroga, T Kadosawa, M Okumura, T Fujinaga JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE 46 (9) 573 -579 1999年11月 [査読無し][通常論文]
     
    The effects of 22-oxacalcitriol (OCT), calcitriol and all-trans retinoic acid (ATRA) on the induction of functional differentiation and growth inhibition of the canine osteosarcoma cell line POS were investigated in vitro via bone differentiation markers and proliferation assays, respectively. The intracellular alkaline phosphatase (ALP) activity and the gamma-carboxyglutamic acid osteocalcin (GLA-OC) and procollagen type I C peptide (PIP) production were used as markers of differentiation. Treatment with 10(-8) M concentrations of all drugs for 72 h significantly inhibited growth (P < 0.0001) and increased ALP activity and GLA-OC and PIP production in POS. OCT, calcitriol and ATRA significantly increased the: ALP activity from 1.58 +/- 0.14 mu mol/min/mg protein (mean +/- SD; control) to 2.50 +/- 0.09 (P < 0.0001), 2.30 +/- 0.14 (P < 0.0001) and 2.00 +/- 0.14 (P = 0.0008), respectively; GLA-OC production from 0.71 +/- 0.01 ng/ml (control) to 2.87 +/- 0.01 (P < 0.0001), 2.87 +/- 0.11 (P < 0.0001) and 1.36 +/- 0.06 (P < 0.0001), respectively; and PIP production From 433.91 +/- 23.29 ng/ml (control) to 536.54 +/- 15.46 (P = 0.0002); 497.06 +/- 1.99 (P = 0.0028) and 481.66 +/- 0.01 (P = 0.0104), respectively. This stud! demonstrated that treatment with these drugs induced a phenotypic maturation of POS cells into cells that exhibit the properties of functionally mature bone cells a with parallel growth inhibition. The effects of these drugs on functional differentiation may be useful to complement the progression of a normal osteogenic differentiation process in the sarcoma.
  • 子牛の実験的肺炎における臨床所見と動脈血液ガス,白血球数及び血清ハプトグロビン濃度の推移
    大崎 和栄, 草場 信之, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 128回 233 -233 1999年09月 [査読無し][通常論文]
  • 関節周囲の骨折と手術法 関節及び関節周囲の骨折の整復と救済的治療法
    奥村 正裕, 廉澤 剛, 藤永 徹 獣医麻酔外科学雑誌 30 (Suppl.2) 53 -54 1999年08月 [査読無し][通常論文]
  • イヌの根尖部歯周組織再建へのエナメルマトリックスタンパクの応用
    渡邊 一弘, 菊池 正浩, 奥村 正裕, 廉澤 剛, 藤永 徹 獣医麻酔外科学雑誌 30 (Suppl.2) 133 -133 1999年08月 [査読無し][通常論文]
  • 慢性多発性神経根炎のイヌの1症例
    石黒 武人, 廉澤 剛, 相川 剛, 岩田 奈織子, 落合 謙爾, 梅村 孝司, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 30 (Suppl.2) 129 -129 1999年08月 [査読無し][通常論文]
  • ノカルジア感染による肉芽腫のイヌの1治験例
    上野 博史, 廉澤 剛, 落合 謙爾, 梅村 孝司, 神谷 和宏, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 30 (Suppl.2) 125 -125 1999年08月 [査読無し][通常論文]
  • 肥満細胞腫罹患犬における血中ヒスタミン濃度の推移と予後に関する検討
    廉澤 剛, 岩田 奈織子, 落合 謙爾, 梅村 幸司, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 43 (8) 279 -279 1999年08月 [査読無し][通常論文]
  • 下顎に発生した造骨型扁平上皮癌のネコの1例
    神谷 和宏, 廉澤 剛, Ekowati H, 落合 謙爾, 梅村 孝司, 鳥越 慎吾, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 43 (8) 273 -273 1999年08月 [査読無し][通常論文]
  • 両側上腕関節窩靱帯の再建術を行った肩甲上腕関節不安定症のイヌの1例
    堤 奈々恵, 奥村 正裕, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 43 (8) 270 -270 1999年08月 [査読無し][通常論文]
  • 緩徐な進行を示した腰椎原発の骨肉腫を発症したイヌの1例
    高木 哲, 廉澤 剛, 佐藤 和男, 落合 謙爾, 磯村 洋, 梅村 孝司, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 43 (8) 277 -277 1999年08月 [査読無し][通常論文]
  • 肘異形成を伴う習慣性肘関節脱臼に側副靱帯再建及び関節固定ピンを適用した犬の1例
    奥村 正裕, 堤 奈々恵, 志尾 大一, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 43 (8) 270 -270 1999年08月 [査読無し][通常論文]
  • 放射線治療を行った猫の鼻腔リンパ腫の7症例
    廉澤 剛, 落合 謙爾, 梅村 孝司, 磯村 洋, 柄本 浩一, 高橋 徹, 玉井 聡, 鳥越 慎吾, 星野 佐登志, 奥村 正裕 北海道獣医師会雑誌 43 (8) 274 -274 1999年08月 [査読無し][通常論文]
  • 「ウマの実験的関節炎に対するヒアルロン酸ナトリウム静脈内投与の治療効果.」
    渡邊 一弘, 奥村 正裕, 廉澤 剛, 藤永 徹 動薬研究 59 (8) 1 -10 1999年08月 [査読無し][通常論文]
  • 「犬の関節疾患に対する緑イ貝抽出物経口投与の治療効果-臨床学的観察-.」
    奥村 正裕, 上野 博史, 渡邊 一弘, 廉澤 剛, 島田 健次郎, 藤永 徹 小動物臨床 18 (4) 5 -14 1999年07月 [査読無し][通常論文]
  • 浅野 和之, 増田 桂子, 奥村 正裕, 廉澤 剛, 藤永 徹 The Journal of Veterinary Medical Science 61 (5) 523 -529 1999年05月 [査読無し][通常論文]
  • Barroga Edward F, 廉澤 剛, 奥村 正裕, 藤永 徹 The Journal of Veterinary Medical Science 61 (4) 361 -367 1999年04月 [査読無し][通常論文]
  • イヌ肥満細胞腫の血中ヒスタミン動態に関する検討
    石黒 武人, 廉澤 剛, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 127回 167 -167 1999年03月 [査読無し][通常論文]
  • In vitroにおけるキトサンによるマクロファージ活性化に関する研究
    木内 武雄, 奥村 正裕, 森 崇, 上野 博史, 浅野 和之, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 127回 186 -186 1999年03月 [査読無し][通常論文]
  • 浅野 和之, 廉澤 剛, 奥村 正裕, 藤永 徹 The Journal of Veterinary Medical Science 61 (1) 89 -91 1999年01月 [査読無し][通常論文]
  • 渡邊 一弘, 廉澤 剛, 宮田 佳代子, 奥村 正裕, 藤永 徹 獣麻酔外科誌 30 (1/2) 35 -41 1999年 [査読無し][通常論文]
  • Barroga, E. F., Kadosawa, T., Okumura, M., Fujinaga, T. : "In vitro of canine osteosarcoma by 22-oxacalcitriol, calcitriol and all-trans retinoic acid", J. Vet. Med. A, 46:573-579(1999)
    1999年 [査読無し][通常論文]
  • Ueno, H., Yamada, H., Tanaka, I., Kaba, N., Matsuura, M., Okumura, M., Kadosawa, T., Fujinaga, T. : "Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs", Biomaterials, 20:1407-1414(1999)*
    1999年 [査読無し][通常論文]
  • Asano, K., Kadosawa, T., Okumura, M., Fujinaga, T. : "Peri-operative changes in echocardiographic measurements and plasma atrial and brain natriuretic peptide concentrations in 3 dogs with patent ductus arteriosus", J. Vet. Med. Sci., 61(1): 89-91(1999)*
    1999年 [査読無し][通常論文]
  • Okumura, M., Kadosawa, T., Fujinaga, T.: "Surgical correction of temporomandibular joint ankylosis in two cats", Aust. Vet. J., 77(1): 24-27(1999)*
    1999年 [査読無し][通常論文]
  • Asano, K., Masuda, K., Okumura, M., Kadosawa, T., Fujinaga, T. : "Plasma artrial and brain natriuretic peptide levels in dogs with congestive heart failure", J. Vet. Med. Sci., 61:523-529(1999)*
    1999年 [査読無し][通常論文]
  • Barroga, E. F., Kadosawa, T., Okumura, M., Fujinaga, T. : "Establishment and characterization of the growth and pulmonary metastasis of a highly lung metestasizing cell line from canine osteosarcoma in nude mice", J. Vet. Med. Sci., 61: 361-367(1999)*
    1999年 [査読無し][通常論文]
  • Barroga, E. F., Kadosawa, T., Okumura, M., Fujinaga, T. : "Effects of vitamin D and retinoids on the differentiation and growth in vitro of canine osteosarcoma and its clonal cell lines", Res. Vet. Sci., 66: 231-236(1999)*
    1999年 [査読無し][通常論文]
  • Barroga Edward F, 廉澤 剛, 奥村 正裕, 藤永 徹 The Journal of Veterinary Medical Science 60 (11) 1269 -72 1998年11月 [査読無し][通常論文]
     
    Vitamin D3: 1-alpha, 25(OH)2D3 (calcitriol), 22-oxa-1,25(OH)2D3 (OCT), cholecalciferol (vitamin D3), and retinoids: all-trans retinoic acid (ATRA) and 9-cis retinoic acid, induced morphological changes in POS canine osteosarcoma cells into elongated, spindle or fibroblast like-shaped cells, and apoptotic like cell death characterized by cell shrinkage, condensation and margination of the nucleus for all drugs at 10(-6)M-10(-9)M after 72 to 120 hr culture. Apoptosis as shown by DNA laddering was induced at 48 hr by all drugs at 10(-6)M, 10(-7)M at 96 hr, 10(-8)M and 10(-9)M at 120 hr respectively. These vitamins are suggested to adjunct antineoplastic agents in canine osteosarcoma therapy by induction of apoptosis.
  • 呼吸不全を伴った頸髄髄膜腫のイヌの一治験例
    廉澤 剛, 浅野 和之, 森 崇, 伊藤 博章, 奥山 卓郎, 落合 謙爾, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 29 (Suppl.2) 53 -53 1998年10月 [査読無し][通常論文]
  • 浅野 和之, 奥村 正裕, 宮本 徹, 森 崇, 廉澤 剛, 藤永 徹 日本獣医師会雑誌 51 (10) 595 -598 1998年10月 [査読無し][通常論文]
  • 髄内ピンと金属パテで治療した中足骨複雑骨折のイヌの1例
    奥村 正裕, 渡邊 一弘, 上野 博史, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 42 (10) 345 -349 1998年10月 [査読無し][通常論文]
  • M Okumura, M Tagami, T Fujinaga JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE 45 (8) 513 -516 1998年10月 [査読無し][通常論文]
     
    Keratan sulphate (KS) concentration and anticollagen type II antibody levels were measured in the serum and synovial fluid(SF) of clinically normal horses and horses with osteoarthritis (OA). Serum KS in OA was significantly higher than that in normal horses, white no significant difference was found in KS levels of SF between normal and OA. Assays of antibody to collagen type II showed no significant increase in sera and SF of OA. It was suggested that levels of serum KS would be of value in the pathological detection of OA in the joint, although there was no evidence that the measurable autoimmunity to collagen antigens would reflect the process of OA.
  • 肺原発悪性組織球症のイヌの一例
    福井 大祐, 廉澤 剛, 浅野 和之, 落合 謙爾, 相川 剛, 奥村 正裕, 藤永 徹 北獣会誌 42 (9) 321 -323 1998年09月 [査読無し][通常論文]
  • 放射線で治療した角膜の扁平上皮癌のイヌの1症例
    伊藤 直子, 廉澤 剛, 小谷 忠生, 落合 謙爾, 上野 博史, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 42 (8) 297 -297 1998年08月 [査読無し][通常論文]
  • 横紋筋肉腫が肛門周囲に発生したイヌの1治験例
    上野 博史, 廉澤 剛, 落合 謙爾, 岡田 由紀, 磯村 洋, 諏訪 義典, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 42 (8) 299 -299 1998年08月 [査読無し][通常論文]
  • イヌの頸部にみられた骨外性骨肉腫の1症例
    狭間 律子, 廉澤 剛, 落合 謙爾, 渡邊 一弘, 岩田 奈緒子, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 42 (8) 280 -280 1998年08月 [査読無し][通常論文]
  • ペンス・ジョーンズ型多発性骨髄腫が疑われたイヌの1例
    石黒 武人, 廉澤 剛, 落合 謙爾, 浅野 和之, 前谷 茂樹, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 42 (8) 273 -273 1998年08月 [査読無し][通常論文]
  • 髄内ピンと金属用パテを用いて整復した中足骨複雑骨折の犬1例
    奥村 正裕, 渡邊 一弘, 上野 博史, 森 崇, 廉澤 剛, 藤永 徹 北海道獣医師会雑誌 42 (8) 259 -259 1998年08月 [査読無し][通常論文]
  • 骨嚢腫が疑われたイヌの1症例
    渡邊 一弘, 廉澤 剛, 落合 謙爾, 浅野 和之, 田村 誠朗, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 42 (8) 276 -276 1998年08月 [査読無し][通常論文]
  • 頸髄の動静脈奇形に起因する神経症状を呈したイヌの1例
    木内 武雄, 廉澤 剛, 斉藤 博司, 浅野 和之, 石黒 武人, 奥村 正裕, 落合 謙爾, 藤永 徹 北海道獣医師会雑誌 42 (8) 285 -285 1998年08月 [査読無し][通常論文]
  • イヌの動脈管開存症(PDA)の周術期における心エコー検査所見及び血漿ナトリウム利尿ペプチド濃度の変化
    浅野 和之, 廉澤 剛, 狭間 律子, 増井 優里, 立花 徹, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 42 (8) 284 -284 1998年08月 [査読無し][通常論文]
  • 抗がん剤を腹腔内投与した悪性腫瘍のイヌとネコの各1例
    廉澤 剛, 宮本 徹, 浅野 和之, 落合 謙爾, 小西 正純, 天内 満雄, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 42 (8) 271 -271 1998年08月 [査読無し][通常論文]
  • ネコの皮膚におけるMRSA感染症の1例
    増井 優里, 上野 博史, 廉澤 剛, 奥村 正裕, 藤永 徹 北海道獣医師会雑誌 42 (8) 264 -264 1998年08月 [査読無し][通常論文]
  • ミエロペルオキシダーゼ反応の一重項酸素の検出
    桐生 千花, 牧内 正男, 宮崎 潤二, 奥村 正裕, 廉澤 剛, 藤永 徹, 柿沼 カツ子 日本獣医学会学術集会講演要旨集 126回 148 -148 1998年08月 [査読無し][通常論文]
  • イヌの全歯根における根尖部apical deltaの観察
    渡邊 一弘, 菊池 正浩, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 126回 163 -163 1998年08月 [査読無し][通常論文]
  • 連続波ドプラ心エコー法を用いたイヌの心収縮機能の評価法の検討
    増井 優里, 浅野 和之, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 126回 70 -70 1998年08月 [査読無し][通常論文]
  • 高密度Pellet培養法を用いたウマ関節軟骨細胞におけるプロテオグリカン代謝の評価
    奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 126回 172 -172 1998年08月 [査読無し][通常論文]
  • 宮本 徹, 白浜 美帆, 桐生 千花, Timothy Mwanza, 奥村 正裕, 藤永 徹 獣医麻酔外科学雑誌 29 (3) 73 -82 1998年07月 [査読無し][通常論文]
  • キトサンによる好中球のオステオポンチン発現に対する影響
    上野 博史, 上出 利光, 山田 明夫, 磯村 洋, 蒲 直樹, 田中 一郎, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 125回 240 -240 1998年03月 [査読無し][通常論文]
  • イヌの脳性ナトリウム利尿ペプチド遺伝子のクローニング
    浅野 和之, 村上 正晃, 遠藤 大二, 木村 享史, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 125回 267 -267 1998年03月 [査読無し][通常論文]
  • イヌ再植歯を実験系とした歯周組織の再建 コラーゲン線維膜とBMPの組織工学的応用
    渡邊 一弘, 菊池 正浩, 滝田 裕子, 久保木 芳徳, 大畑 昇, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 125回 281 -281 1998年03月 [査読無し][通常論文]
  • イヌの腫瘍における放射線治療効果の予測因子としてのアポトーシスに関する検討
    市川 美佳, 廉澤 剛, 奥村 正裕, 藤永 徹 日本獣医学会学術集会講演要旨集 125回 242 -242 1998年03月 [査読無し][通常論文]
  • 実験的僧帽弁逆流作出犬を用いた慢性心不全モデルにおける心房性ナトリウム利尿ペプチド投与の有用性
    増田 佳子, 浅野 和之, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 125回 279 -279 1998年03月 [査読無し][通常論文]
  • イヌにおけるキトサンの皮下大量投与後の好中球に起因する肺障害
    福井 大祐, 森 崇, 上野 博史, 落合 謙爾, 蒲 直樹, 田中 一郎, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 125回 240 -240 1998年03月 [査読無し][通常論文]
  • キチン・キトサンのマウス常在腹腔マクロファージに対する作用
    森 崇, 村上 正晃, 上出 利光, 蒲 直樹, 田中 一郎, 奥村 正裕, 廉澤 剛, 藤永 徹 日本獣医学会学術集会講演要旨集 125回 275 -275 1998年03月 [査読無し][通常論文]
  • 髄内ピンと金属用パテで治療した中足骨複雑骨折のイヌの1例
    北獣会誌 42 (10) 345 -349 1998年 [査読無し][通常論文]
  • 日獣会誌 51 (10) 595 -598 1998年 [査読無し][通常論文]
  • 放射線療法が奏効したネコの鼻腔内腫瘍の一例
    北獣会誌 42 (7) 156 -159 1998年 [査読無し][通常論文]
  • 宮本 徹, 白浜 美帆, 桐生 千花, 奥村 正裕, 藤永 徹 獣麻酔外科誌 29 (3) 73 -82 1998年 [査読無し][通常論文]
  • Okumura M, Fujinaga T. Establishment of a monoclonal antibody (1/14/16H9) for detection of equine keratan sulfate. Am J Vet Res 59(10): 1203-1208 (1998)*
    1998年 [査読無し][通常論文]
  • Mwanza T, Miyamoto T, Okumura M, Kadosawa T, Fujinaga T. Ultrasonographic evaluation of portal vein hemodynamics in experimentally bile duct ligated dogs. Jpn J Vet Res 45(4): 199-206 (1998)*
    1998年 [査読無し][通常論文]
  • Okumura M, Asano M, Tagami M, Tsukiyama K, Fujinaga t. Serum copper and ceruloplasmin activity as the early growing stage in foals. Can J Vet Res 62(2): 122-126 (1998)*
    1998年 [査読無し][通常論文]
  • Barroga EF, Kadosawa T, Okumura M, Fujinaga T. Apoptosis in duction of POS canine osteosarcoma cells by vitamin D and retinoids. J Vet Med Sci 60: 1269-1272 (1998)*
    1998年 [査読無し][通常論文]
  • Mori T, Irie Y, Nishimura SI, Tokura S, Matsuura M, Okumura M, Kadosawa T, Fujinaga T. Endocelial cell responses to chitin and its derivertives. J Biomed Mater Res 43(4): 469-472 (1998)*
    1998年 [査読無し][通常論文]
  • T Mwanza, T Miyamoto, M Okumura, T Kadosawa, T Fujinaga JAPANESE JOURNAL OF VETERINARY RESEARCH 45 (3) 153 -161 1997年11月 [査読無し][通常論文]
     
    The results of liver function tests and ultrasonographical findings were analysed in 7 dogs that were intravenously injected dimethylnitrosamine (DMNA 2 mg/kg body weight) on 2 consecutive days each week for 10 weeks. Typical clinical signs and similar changes in liver enzyme concentrations that develop in dogs with natural cirrhosis were observed in this canine model. Severe anaemia and a significant reduction in the platelet numbers occurred in the dogs that died in the 5th week, while in all the other dogs these parameters decreased slightly. Serum total protein and the albumin/globulin ratio decreased gradually while the alkaline phosphatase, alanine amino transferase, aspartate amino transferase and gamma glutamyl transpeptidase activities increased significantly (p<0.05) in all dogs after beginning the administration of DMNA. Ultrasound findings of a coarsened and heterogeneous echo pattern with increased echogenicity that are characteristic of canine cirrhosis were noticed at the same time when the changes in liver enzymes became evident. Present results suggest that ultrasonography in conjunction with liver function tests may be useful in the evaluation of experimentally induced liver cirrhosis.
  • 犬の骨軟骨症(Osteochondrosis) その原因と病態について
    奥村 正裕 北海道獣医師会雑誌 41 (10) 355 -361 1997年10月 [査読無し][通常論文]
  • ウマ軟骨由来ケラタン硫酸に対するモノクローナル抗体(1/14/16H9)の開発
    奥村 正裕 日本獣医学会学術集会講演要旨集 124回 201 -201 1997年09月 [査読無し][通常論文]
  • 奥村 正裕, 浅野 玄, 藤永 徹 The Journal of Veterinary Medical Science 59 (2) 137 -139 1997年02月 [査読無し][通常論文]
  • 「肩甲骨関節上結節剥離骨折により肩関節の不安定を呈したイヌの1例」
    奥村 正裕, 宮本 徹, 森 崇 『北獣会誌』 41 (2) 38 -40 1997年02月 [査読無し][通常論文]
  • M Okumura, M Asano, T Fujinaga JOURNAL OF VETERINARY MEDICAL SCIENCE 59 (2) 137 -139 1997年02月 [査読無し][通常論文]
     
    The optimum pH for the measurement of serum p-phenylenediamine oxidase (Ox) activity was given (pH 6.6), and the relationship between serum Ceruloplasmin(Cp) concentration and its Ox activity was established in healthy adult horses. In adult horses, serum antigenic Cp concentrations were measured by the single radial immunodiffusion (SRID) method with the affinity-purified antibody to equine plasma Cp and compared with its Ox activity. Efficient co-relation between Cp concentration and Ox activity in the sera (r=0.93) and its Ox/Cp ratio were given. These results might contribute to the calculation of antigenic Cp concentration from its Ox activity, the analysis of holo-Cp content in serum, and the research for copper metabolism in Thoroughbred horses.
  • Mori, T., Okumura, M., Matsuura, M., Ueno, K., Tokura, S., Okamoto, Y., Minami, S. and Fujinaga, T. : "Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro", Biomaterials, 18 : 947-951 (1997)*
    1997年 [査読無し][通常論文]
  • Miyamoto, T., Shirahama, M., Kiryu, C., Mwanza, T., Okumura, M., Hagio, M. and Fujinaga, T. : "Comparison of systemic and renal hemodynamics measured by doppler ultrasonography in canine experimental hypovolemia", J. Vet. Med. Sci., 59 : 347-352 (1997)*
    1997年 [査読無し][通常論文]
  • Okumura, M., Miyata, K., Kadosawa, T. and Fujinaga, T. : "Improvement of ELISA for the concentrations of immunogenic keratan sulfate in sera and synovial fluid from horses : Establishment of a monoclonal antibody 1/14/16H9 reacting with equine keratan ・・・
    1997年 [査読無し][通常論文]
     
    Okumura, M., Miyata, K., Kadosawa, T. and Fujinaga, T. : "Improvement of ELISA for the concentrations of immunogenic keratan sulfate in sera and synovial fluid from horses : Establishment of a monoclonal antibody 1/14/16H9 reacting with equine keratan sulfate", Rivista SIDI, 3 : 79 (1997)
  • Mwanza, T., Miyamoto, T., Okumura, M., Kadosawa, T. and Fujinaga, T. : "Ultrasonography, biochemical and hematological profilrs in liver disease caused by intravenous administration of dimethylnitrosamine in dogs", Jpn. J. Vet. Res., 45 : 153-161 (1997)*
    1997年 [査読無し][通常論文]
  • Okumura, M., Fujinaga, T., Urakawa, E., Tagami, M. and Tsukiyama, K. : "Evaluation of the catabolic activity of cartilage by measurement of serum keratan sulfate concentration in foals", Am. J. Vet. Res., 58 : 925-929 (1997)*
    1997年 [査読無し][通常論文]
  • 犬の骨軟骨症(Osteochondrosis) -病因と病態について-
    北獣会誌 41 355 -361 1997年 [査読無し][通常論文]
  • Miyamoto T, Shirahama M, Kiryu C, Mwanza T, Okumura M, Hagio M, Fujinaga T. Comparison of systemic and renal hemodynamics measured by doppler ultrasonography in canine experimental hypovolemia. J Vet Med Sci 59(5): 347-352 (1997)
    1997年 [査読無し][通常論文]
  • Mori T, Okumura M, Matsuura M, Ueno K, Tokura S, Okamoto Y, Minami S, Fujinaga T. Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro. Biomaterials 18(13): 947-951 (1997)
    1997年 [査読無し][通常論文]
  • Okumura M, Asano M, Fujinaga T. Consideration of the optimum pH for the analysis of serum p-phenylenediamine oxidase activity in thoroubred horses. J Vet Med Sci 59(2): 137-139 (1997)
    1997年 [査読無し][通常論文]
  • Okumura M, Fujinaga T, Urakawa E, Tagami M, Tsukiyama K, Evaluation of the catabolic activity at early growing stage in foals. Am J Vet Res 58(8): 925-929 (1997)
    1997年 [査読無し][通常論文]
  • 小動物 術後急速な神経症状の改善を認めた椎間板ヘルニアの1例
    森 崇, 奥村 正裕, 宮本 徹 北海道獣医師会雑誌 40 (10) 277 -279 1996年10月 [査読無し][通常論文]
  • 肩甲上結節の剥離骨折により肩関節の不安定を示した犬の1例
    奥村 正裕 北海道獣医師会雑誌 40 (8) 233 -233 1996年08月 [査読無し][通常論文]
  • 森 崇, 奥村 正裕, 宮本 徹, 浅野 和之, 小賀野 淳, 大場 雅予, 角川 雅俊, 日登 弘, 藤永 徹 『獣医麻酔外科学会誌』 27 (2) 57 -63 1996年04月 [査読無し][通常論文]
  • 新しい超音波造影剤の獣医療への応用
    ProVet 12 32 -38 1996年 [査読無し][通常論文]
  • Mwanza T, Miyamoto T, Okumura M, Hagio M, Fujinaga T. Ulttasonography and angiographic examination of normal canine liver vessels. Jpn J Vet Res 44(3): 179-188 (1996)
    1996年 [査読無し][通常論文]
  • セイウチ(Odobenus rosmarus)のイソフルラン全身麻酔下における上顎犬歯抜歯術の1例
    獣麻酔外科誌 27 57 -63 1996年 [査読無し][通常論文]
  • 術後急速な神経症状の改善を認めた椎間板ヘルニアの1例
    北獣会誌 40 277 -279 1996年 [査読無し][通常論文]
  • 「術後急速な神経症状の改善を認めた椎間板ヘルニアの1例」
    『北海道獣医師会雑誌』 40 277 -279 1996年 [査読無し][通常論文]
  • 「新しい超音波造影剤の獣医臨床への応用」
    『Pro. Vet.』 12 32 -38 1996年 [査読無し][通常論文]
  • T. Mwanza, T. Miyamoto, M. Okumura, M. Hagio and T. Fujinaga : "Ultrasonography and Anigiographic Examination of Normal Canine Liver Vessels", Japanese Journal of Veterinary Research, 44 : 179-188 (1996)*
    1996年 [査読無し][通常論文]
  • 『救急医療』<藤永 徹>
    学窓社 1996年 [査読無し][通常論文]
  • ウマ培養関節軟骨細胞のサイトカイン刺激に対する反応性
    奥村 正裕 日本獣医学会学術集会講演要旨集 120回 157 -157 1995年11月 [査読無し][通常論文]
  • 鼓室胞の骨融解を伴う中耳疾患のネコ2例
    奥村 正裕 日本獣医師会雑誌 48 (2) 119 -119 1995年02月 [査読無し][通常論文]
  • Miyamoto T, Hagio M, Mwanza T, Kobayashi T, Okumura M, Fujinaga T. Quantitative measurement of canine renal arterial blood flow using doppler ultrasonography. J Vet Med Sci 57(4): 785-788 (1995)
    1995年 [査読無し][通常論文]
  • Miyamoto T, Hagio M, Mwanza T, Okumura M, Fujinaga T. Compression of spinal cord caused by spinal deformation in a cat. J Vet Med Sci 57(6): 1101-1103 (1995)
    1995年 [査読無し][通常論文]
  • Satoh M, Fujinaga T, Okumura M, Hagio M. Sandwich enzyme-linked immunosorbent assay for quantitative measurement of serum amyloid A protein in horses. Am J Vet Res 56(10): 1286-1291 (1995)
    1995年 [査読無し][通常論文]
  • 馬血中ケラタン硫酸量測定の臨床学的意義について
    奥村 正裕 獣医麻酔外科学雑誌 25 (Suppl.2) 23 -23 1994年11月 [査読無し][通常論文]
  • 鼓室胞の骨融解を伴う中耳疾患のネコ2例
    奥村 正裕 北海道獣医師会雑誌 38 (8) 262 -262 1994年08月 [査読無し][通常論文]
  • 顎関節手術を行ったネコ、イヌの各1例
    奥村 正裕, 萩尾 光美, 大洞 嗣子 北獣会誌 38 (7) 138 -141 1994年07月 [査読無し][通常論文]
  • 顎関節手術を行った開口不全のネコ,イヌの各1例
    奥村 正裕 日本獣医師会雑誌 47 (2) 131 -131 1994年02月 [査読無し][通常論文]
  • 唾液腺造影が診断上有用であったイヌの唾液腺のう胞と耳下腺腫瘍の各1例
    北獣会誌 38 70 -73 1994年 [査読無し][通常論文]
  • 顎関節手術を行った開口不全のネコ,イヌの各1例
    奥村 正裕 北海道獣医師会雑誌 37 (9) 315 -315 1993年09月 [査読無し][通常論文]
  • Nunokawa Y, Fujinaga T, Taira T, Okumura M, Yamashita K, Tsunoda N, Mizuno S. Evaluation of serum amyloid A protein as an acute-phase reactive protein in horses. J Vet Med Sci 55: 1011-1016 (1993)
    1993年 [査読無し][通常論文]
  • ウマの急性相反応蛋白
    奥村 正裕, 山下 和人, 平 知子 家畜生化学研究会報 29 (29) 9 -18 1992年09月 [査読無し][通常論文]
  • T TAIRA, T FUJINAGA, K TAMURA, M IZUMI, H ITOH, N TSUNODA, K YAMASHITA, M OKUMURA, S MIZUNO AMERICAN JOURNAL OF VETERINARY RESEARCH 53 (6) 961 -965 1992年06月 [査読無し][通常論文]
     
    Taira T, Fujinaga T, Tamura K, Izumi M, Itoh H, Tsunoda N, Yamashita K, Okumura M, Mizuno S. Isolation, characterization of alpha1-acid glycoprotein from horses, and its evaluation as an acute-phase reactive protein in horses.
    Am J Vet Res 53: 961-965 (1992)
  • 平 知子, 藤永 徹, 奥村 正裕 The Journal of Veterinary Medical Science 54 (3) 435 -442 1992年 [査読無し][通常論文]
     
    Haptoglobin (Hp) was isolated from equine serum by ammonium sulphate precipitation, anion-exchange chromatography and gel filtration. Equine Hp which migrated to the α2-globulin region in electrophoresis, contained 2 fractions with molecular weights (NW) of 108,000 and 105,000, and each fraction consisting of 2 subunits. Quantitative measurement of Hp in equine serum was performed by the single radial immunodiffusion method using anti-equine Hp serum. In clinically normal horses, the highest concentration of serum Hp was found in newborn foals and a high value was maintained until 12 months of age. The concentration then decreased with age. Normal Hp values were 5.25±2.36 mg/ml in foals (≦12 months old), 2.19±1.54 mg/ml in adult horses (≧18 months old) and 3.62±0.81 mg/m/ in all horses. Serum Hp concentration in mares during the perinatal period in comparison with the normal adult female was high for 4 months pre-partum, a passing increase at delivery, and then decreased at 2 weeks post-partum returning to normal within 1 month of delivery. In horses with experimentally-induced inflammation, serum Hp concentration began to increase immediately after treatment and reached the highest value, 1.5 to 9 times higher than those of pre-treatment at 2 to 5 days, then decreased within 4 weeks. It was also elevated in most cases of horses with clinically inflammatory signs. © 1992, JAPANESE SOCIETY OF VETERINARY SCIENCE. All rights reserved.
  • Taita T, Fujinaga T, Okumura M, Yamashita K, Tsunoda N, Mizuno S. Equine haptoglobin: Isolation, characterization, and the effects of aging, delivery and inflammation on its serum concentration. J. Vet. Med. Sci. 54: 435-442 (1992)
    1992年 [査読無し][通常論文]
  • 山下 和人, 藤永 徹, 奥村 正裕 The Journal of Veterinary Medical Science 53 (6) 1019 -1024 1991年12月 [査読無し][通常論文]
  • 北海道大学獣医学部附属家畜病院外科における野生傷病鳥獣診療記録(3)寄生虫(線虫)性皮膚炎を発症した野生エゾタヌキの一例
    野村 卓司, 奥村 正裕, 井上 久美子 北海道獣医師会雑誌 35 (6) 157 -160 1991年06月 [査読無し][通常論文]
  • 北海道大学獣医学部附属家畜病院における野生傷病鳥獣診療記録 3.寄生虫(線虫)性皮膚炎を発症した野生エゾタヌキの1例
    北獣会誌 35 157 -160 1991年 [査読無し][通常論文]
  • Yamashita K, Fujinaga T, Okumura M, Takiguchi M, Tsunoda N, Mizuno S. Serum C-reactive protein (CRP) in horses: The effect of aging, sex, delivery and inflammation on its serum concentration. J. Vet. Med. Sci. 53: 157-160 (1991)
    1991年 [査読無し][通常論文]
  • Okumura M, Fujinaga T, Yamashita K, Tsunoda N, Mizuno S. Isolation, characterization, and quantitative analysis of ceruloplasmin from horses. Am J Vet Res 52(12): 1979-1985 (1991)*
    1991年 [査読無し][通常論文]
  • オオハクチョウに発生した鉛中毒の臨床経過と治療の試み
    山下 和人, 井上 久美子, 奥村 正裕 北海道獣医師会雑誌 34 (4) 85 -89 1990年04月 [査読無し][通常論文]
  • ウマの急性相蛋白に関する研究(2) ウマ血漿セルロプラスミンの分離・精製とその定量
    奥村 正裕 日本獣医学会学術集会講演要旨集 109回 254 -254 1990年03月 [査読無し][通常論文]
  • 山下 和人, 水野 信哉, 井上 久美子, 奥村 正裕, 成清 美智代, 浅野 和之, 小林 俊明, 平 知子, 野村 卓司, 藤永 徹 獣麻酔外科誌 21 (4) 85 -91 1990年 [査読無し][通常論文]
  • 北海道大学獣医学部附属家畜病院における野生傷病鳥獣診療記録 2.釣針を誤飲した野生水鳥の3例
    北獣会誌 34 161 -163 1990年 [査読無し][通常論文]
  • 北海道大学獣医学部附属家畜病院における野生傷病鳥獣診療記録 1.オオハクチョウに発生した鉛中毒の臨床経過と治療の試み
    北獣会誌 34 85 -89 1990年 [査読無し][通常論文]
  • イヌの皮下に発生した巨大な血管肉腫の一例
    日獣会 42 491 -493 1989年 [査読無し][通常論文]

共同研究・競争的資金等の研究課題

  • 動物の関節疾患における硫酸化グリコサミノグリカンの作用発現機序
    研究期間 : 2008年
  • 骨・軟骨代謝マーカーを用いた馬の骨格に対する運動負荷の解析
    その他の研究制度
    研究期間 : 2007年
  • 犬の滑膜における外因性ヒアルロン酸の作用
    研究期間 : 2006年
  • 犬の自然発生腫瘍に対する免疫学的治療法の適用
    その他の研究制度
    研究期間 : 2003年
  • 動物の腫瘍性疾患における光線力学的抗腫瘍療法の応用
    研究期間 : 2000年
  • 関節軟骨代謝変動と損傷関節軟骨再生
    研究期間 : 1993年
  • Changes of articular cartilage metabolism in animals and biological reconstrucition of articular cartilage defects
    研究期間 : 1993年

教育活動情報

主要な担当授業

  • 獣医科学特別研究
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 軟部組織外科学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 軟部組織、眼科、外科適応疾患、麻酔法、保存療法、手術療法、術後管理
  • 獣医科学特論演習
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 外科学総論
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 獣医外科学、外科的侵襲、病態生理、損傷、創傷
  • 先端獣医科学特論A 最新獣医外科学特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 臨床腫瘍学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 臨床獣医学、獣医腫瘍学
  • 先端獣医科学特論A 国際獣医科学特論:臨床獣医学
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学研究科
  • 整形外科学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 獣医整形外科学、骨、関節、靭帯、腱、蹄
  • 獣医科学特別研究
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 伴侶動物夜間・救急獣医療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 夜間診療、救急処置
  • 獣医科学特論演習
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 伴侶動物獣医療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 伴侶動物、内科、外科、検査、診断、治療
  • 獣医科学基礎科目 臨床疾病学特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 応用外科学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 応用 外科
  • 先端獣医科学科目 最新獣医外科学特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 伴侶動物外科学実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 外科手術、麻酔
  • 総合専門臨床特論
    開講年度 : 2018年
    課程区分 : 博士後期課程
    開講学部 : 獣医学院
  • 産業動物外科学実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
    キーワード : 産業動物(牛、馬)、外科、麻酔
  • 獣医整形外科学
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 獣医臨床総合実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 獣医専門外科学Ⅰ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 獣医専門外科学Ⅱ
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 獣医専門科診療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 伴侶動物獣医療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 伴侶動物夜間・救急獣医療実習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • 研究・臨床セミナー
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部
  • アドバンスト演習
    開講年度 : 2018年
    課程区分 : 学士課程
    開講学部 : 獣医学部


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