研究者基本情報

• 博士（水産科学）, 北海道大学

• https://researchmap.jp/toshiuji55

• https://orcid.org/0000-0002-2925-7913
• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201301094250782816

• 00760597

• https://orcid.org/0000-0002-2925-7913

• 201301094250782816

• 海藻育種
• 植物ホルモン
• アミノシクロプロパンカルボン酸
• 生殖
• ストレス応答
• 海藻類
• 生活環制御
• アマノリ類
• 紅藻類

• ライフサイエンス, 水圏生命科学, 海洋植物学

• 学士課程, 水産学部
• 修士課程, 水産科学院
• 博士課程, 水産科学院

研究活動情報

• 2020年10月, ベストポスター賞 第6回北大・部局横断シンポジウム
• 2011年05月, 平成22年度マリンバイオテクノロジー学会論文賞
  宇治 利樹

• Electroporation-Mediated Gene Delivery in Pyropia yezoensis (Rhodophyta) Without Cell Wall Removal.
  Hikari Izumi; Toshiki Uji; Kojiro Matsumoto; Kaz Nagaosa; Satoru Fukuda
  Marine biotechnology (New York, N.Y.), 27, 5, 123, 123, 2025年08月16日, ［国際誌］
  英語, 研究論文（学術雑誌）, This study aimed to assess the potential of a gene delivery technique in the red macroalga Pyropia yezoensis (Rhodophyta) using electroporation without removing the cell wall. An antibiotic resistance gene was introduced into P. yezoensis tissues containing cells with intact cell walls through electroporation, followed by selection with the corresponding antibiotic. No germlings survived in the non-electroporated control tissue fragments under antibiotic selection. In contrast, some germlings were observed to survive in the electroporated group. Furthermore, the presence of antibiotic resistance genes was confirmed in the genomic DNA of several antibiotic-resistant germlings. Although reporter genes such as β-glucuronidase (GUS) and green fluorescent protein (GFP) were also introduced as supplementary markers, their expression was not detectable under the tested conditions. These findings provide evidence supporting the successful introduction of antibiotic resistance genes into P. yezoensis cells via electroporation. This study offers a preliminary assessment of a gene delivery strategy in P. yezoensis that bypasses cell wall removal, presenting a straightforward method for introducing foreign genes into Pyropia. To the best of our knowledge, this is the first report to demonstrate successful gene transfer via electroporation in a macroalga without cell wall removal. These results provide valuable insights for the development of genetic transformation systems in red macroalgae.
• 1-Aminocyclopropane-1-carboxylic acid induces resource reallocation in Pyropia yezoensis sporophytes
  Toshiki Uji; Shun Sasaki; Hiroyuki Mizuta
  FRONTIERS IN PLANT SCIENCE, 16, 2025年08月15日
  英語, 研究論文（学術雑誌）
• First genetic evidence of interspecific hybridization in the genus Sargassum (Fucales, Phaeophyceae)
  Yuta Jozawa; Sota Minamiguchi; Kaito Hiyoshi; Kenta Chizaki; Chikara Kawagoe; Takahiro Arai; Tatsuya Ishikawa; Toshiki Uji; Hiroyuki Mizuta; Shingo Akita
  PHYCOLOGICAL RESEARCH, 73, 3, 161, 167, 2025年07月
  英語, 研究論文（学術雑誌）
• Isolation and characterization of a spore development mutant in Pyropia yezoensis (Rhodophyta) induced via insertional mutagenesis.
  Hikari Izumi; Yuji Nakai; Toshiki Uji; Kaz Nagaosa; Satoru Fukuda; Hiroyuki Mizuta; Naotsune Saga
  Journal of phycology, 2025年05月05日, ［国際誌］
  英語, 研究論文（学術雑誌）, Mechanistic elucidation of spore development is essential for understanding reproductive strategies and improving seaweed cultivation. In this study, we isolated a mutant with spore development defects in Pyropia yezoensis (Rhodophyta) from among genetic transformants generated via insertional mutagenesis. We characterized the mutant phenotype and identified the genomic region containing the mutation. The mutant produced spores (archeospores, zygotospores, and conchospores) at every relevant stage of the life history. No evident differences were observed in the appearance of the released spores at any stage under light microscopy between the wild-type and mutant strains; however, the mutant exhibited a trend of reduced spore release compared with the wild-type. Additionally, the archeospores released by the mutant remained round after being released from the sporangium, failed to adhere to the substratum, did not germinate, and showed no cell wall development after 4 days of standard culture, in contrast to the wild-type. Similar results were observed for the zygotospores and conchospores. These findings indicate that the main characteristic of this mutant was poor spore release, and the released spores failed to germinate. Whole-genome sequencing revealed the introduction of exogenous DNA into the nuclear genome of the mutant and suggested the presence of duplications, deletion, and inversion in the mutant genome. Notably, a deletion of approximately 13 kb was detected in the mutant genome flanking the insertion, and this region contained a plant homeodomain, suggesting that homeodomain-containing proteins may regulate spore development in P. yezoensis.
• Genetic structure of Saccharina japonica in Japan and evaluation of a potential mitochondrial region for identification of geographic origin
  Kenta Chizaki; Chikara Kawagoe; Keiko Ito; Hiroyuki Mizuta; Yuya Yoshida; Toshiki Uji; Daisuke Fujita; Shingo Akita
  Journal of Applied Phycology, 37, 659, 672, 2025年02月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Identification of differential gene expression related to reproduction in the sporophytes of Saccharina japonica
  Toshiki Uji; Takuya Kandori; Hiroyuki Mizuta
  Frontiers in Plant Science, 15, Frontiers Media SA, 2024年08月06日, ［査読有り］, ［筆頭著者, 責任著者］
  研究論文（学術雑誌）, Saccharina japonica, a significant brown macroalga in the Pacific Ocean, serves as a food source and industrial material. In aquaculture, collecting mature sporophytes for seedling production is essential but challenging due to environmental changes. In this study, transcriptomic analysis of vegetative and sorus tissues was done to identify differentially expressed genes (DEGs) and enhance our understanding of sorus formation regulation in S. japonica. KEGG pathway and Gene Otology (GO) analysis revealed that upregulated DEGs were involved in folate biosynthesis, riboflavin metabolism, and amino acid biosynthesis. In addition, the upregulation of genes associated with cell wall remodeling, such as mannuronan C-5-epimerases, vanadium-dependent haloperoxidases, and NADPH oxidase, was observed in sorus parts. Meanwhile, downregulated DEGs in sorus portions included genes related to chloroplast function. These findings will help us understand the regulatory mechanisms behind sorus formation in S. japonica and extracellular matrix remodeling in brown algae.
• Sustainable protective function of sorus tissue during the alternation of generations in Saccharina japonica
  Kenshiro ABE; Inori ARIGA; Toshiki UJI; Hiroyuki MIZUTA
  Cahiers de Biologie Marine, 65, 73, 85, 2024年01月, ［査読有り］
• Development of Chromatin Immunoprecipitation for the Analysis of Histone Modifications in Red Macroalga Neopyropia yezoensis (Rhodophyta).
  Shinnosuke Ueda; Hiroyuki Mizuta; Toshiki Uji
  Molecular biotechnology, 2022年09月13日, ［国際誌］
  英語, 研究論文（学術雑誌）, Epigenetic regulation by histone modification can activate or repress transcription through changes in chromatin dynamics and regulates development and the response to environmental signals in both animals and plants. Chromatin immunoprecipitation (ChIP) is an indispensable tool to identify histones with specific post-translational modifications. The lack of a ChIP technique for macroalgae has hindered understanding of the role of histone modification in the expression of genes in this organism. In this study, a ChIP method with several modifications, based on existing protocols for plant cells, has been developed for the red macroalga, Neopyropia yezoensis, that consists of a heterogeneous alternation of macroscopic leaf-like gametophytes and microscopic filamentous sporophytes. ChIP method coupled with qPCR enables the identification of a histone mark in generation-specific genes from N. yezoensis. The results indicate that acetylation of histone H3 at lysine 9 in the 5' flanking and coding regions from generation-specific genes was maintained at relatively high levels, even in generation-repressed gene expression. The use of this ChIP method will contribute significantly to identify the epigenetic regulatory mechanisms through histone modifications that control a variety of biological processes in red macroalgae.
• 1-Aminocyclopropane-1-carboxylic acid and its analogs alleviate heat stress damage in the marine red alga Neopyropia yezoensis (Rhodophyta)
  Toshiki Uji; Hiroyuki Mizuta
  JOURNAL OF APPLIED PHYCOLOGY, 34, 3, 1527, 1536, 2022年06月
  英語, 研究論文（学術雑誌）
• Phospholipase D activation is required for 1-aminocyclopropane 1-carboxylic acid signaling during sexual reproduction in the marine red alga Neopyropia yezoensis (Rhodophyta).
  Toshiki Uji; Takuya Kandori; Shiho Konishi; Hiroyuki Mizuta
  BMC plant biology, 22, 1, 181, 181, 2022年04月08日, ［国際誌］
  英語, 研究論文（学術雑誌）, BACKGROUND: 1-aminocyclopropane 1-carboxylic acid (ACC) is the immediate precursor of the plant hormone ethylene. However, recent studies have suggested that ACC also acts as a signaling molecule to regulate development and growth independently from ethylene biosynthesis. In red algae, ACC stimulates the switch from a vegetative to a sexual reproductive phase. However, despite evidence that ACC signaling in plants and algae is widespread, the mechanistic basis of the ACC signaling pathway remains unknown. RESULTS: We demonstrate that exogenous ACC increased the activity of phospholipase D (PLD) and induced the accumulation of PLD transcripts in the marine red alga Neopyropia yezoensis. The product of PLD, the lipid second messenger phosphatidic acid (PA), also increased in response to ACC. Furthermore, the pharmacological inhibition of PLD by 1-butanol blocked ACC-induced spermatangia and carpospore production, but the inactive isomer t-butanol did not. In addition, 1-butanol prevented ACC-induced growth inhibition and inhibited transcript accumulation of genes upregulated by ACC, including extracellular matrix (ECM)-related genes, and alleviated the transcriptional decrease of genes downregulated by ACC, including photosynthesis-related genes. CONCLUSIONS: These results indicate that PLD is a positive regulator of sexual cell differentiation and a negative regulator of growth. This study demonstrates that PLD and its product, PA, are components of ACC signaling during sexual reproduction in N. yezoensis.
• Transcriptome Profiling in the Marine Red Alga Neopyropia yezoensis Under Light/Dark Cycle.
  Sayaka Kominami; Hiroyuki Mizuta; Toshiki Uji
  Marine biotechnology (New York, N.Y.), 24, 2, 393, 407, 2022年04月, ［国際誌］
  英語, 研究論文（学術雑誌）, Many organisms are subjected to a daily cycle of light and darkness, which significantly influences metabolic and physiological processes. In the present study, Neopyropia yezoensis, one of the major cultivated seaweeds used in "nori," was harvested in the morning and evening during light/dark treatments to investigate daily changes in gene expression using RNA-sequencing. A high abundance of transcripts in the morning includes the genes associated with carbon-nitrogen assimilations, polyunsaturated fatty acid, and starch synthesis. In contrast, the upregulation of a subset of the genes associated with the pentose phosphate pathway, cell cycle, and DNA replication at evening is necessary for the tight control of light-sensitive processes, such as DNA replication. Additionally, a high abundance of transcripts at dusk encoding asparaginase and glutamate dehydrogenase imply that regulation of asparagine catabolism and tricarboxylic acid cycle possibly contributes to supply nitrogen and carbon, respectively, for growth during the dark. In addition, genes encoding cryptochrome/photolyase family and histone modification proteins were identified as potential key players for regulating diurnal rhythmic genes.
• The role of plant hormones on the reproductive success of red and brown algae.
  Toshiki Uji; Hiroyuki Mizuta
  Frontiers in plant science, 13, 1019334, 1019334, 2022年, ［国際誌］
  英語, 研究論文（学術雑誌）, Seaweeds or macroalgae are important primary producers that serve as a habitat for functioning ecosystems. A sustainable production of macroalgae has been maintained by a diverse range of life cycles. Reproduction is the most dynamic change to occur during its life cycle, and it is a key developmental event to ensure the species' survival. There is gradually accumulating evidence that plant hormones, such as abscisic acid and auxin, have a role on the sporogenesis of brown alga (Saccharina japonica). Recent studies reported that 1-aminocylopropane-1-carboxylic acid, an ethylene precursor, regulates sexual reproduction in red alga (Neopyropia yezoensis) independently from ethylene. In addition, these macroalgae have an enhanced tolerance against abiotic and biotic stresses during reproduction to protect their gametes and spores. Herein, we reviewed the current understanding on the regulatory mechanisms of red and brown algae on their transition from vegetative to reproductive phase.
• Extracellular silicate uptake and deposition induced by oxidative burst in Saccharina japonica sporophytes (Phaeophyceae)
  Hiroyuki Mizuta; Toshiki Uji; Hajime Yasui
  ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 58, 2021年10月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Treatment with heat shock protein 90 (Hsp90) inhibitors induces asexual life cycle in the marine red alga Neopyropia yezoensis (Rhodophyta)
  Toshiki Uji; Hiroyuki Mizuta
  AQUACULTURE RESEARCH, 52, 12, 6814, 6817, 2021年08月, ［査読有り］, ［筆頭著者, 責任著者］
  英語, 研究論文（学術雑誌）
• α-aminoisobutyric acid mimics the effect of 1-aminocyclopropane-1-carboxylic acid to promote sexual reproduction in the marine red alga Pyropia yezoensis (Rhodophyta)
  Harune Endo; Hiroyuki Mizuta; Toshiki Uji
  Journal of Applied Phycology, 33, 2, 1081, 1087, Springer Science and Business Media B.V., 2021年04月01日
  英語, 研究論文（学術雑誌）
• Characterization of ACE Inhibitory Peptides Prepared from Pyropia pseudolinearis Protein.
  Yuya Kumagai; Keigo Toji; Satoshi Katsukura; Rie Morikawa; Toshiki Uji; Hajime Yasui; Takeshi Shimizu; Hideki Kishimura
  Marine drugs, 19, 4, 2021年04月01日, ［国際誌］
  英語, 研究論文（学術雑誌）, More than 7000 red algae species have been classified. Although most of them are underused, they are a protein-rich marine resource. The hydrolysates of red algal proteins are good candidates for the inhibition of the angiotensin-I-converting enzyme (ACE). The ACE is one of the key factors for cardiovascular disease, and the inhibition of ACE activity is related to the prevention of high blood pressure. To better understand the relationship between the hydrolysates of red algal proteins and the inhibition of ACE activity, we attempted to identify novel ACE inhibitory peptides from Pyropia pseudolinearis. We prepared water soluble proteins (WSP) containing phycoerythrin, phycocyanin, allophycocyanin, and ribulose 1,5-bisphosphate carboxylase/oxygenase. In vitro analysis showed that the thermolysin hydrolysate of the WSP had high ACE inhibitory activity compared to that of WSP. We then identified 42 peptides in the hydrolysate by high-performance liquid chromatography and mass spectrometry. Among 42 peptides, 23 peptides were found in chloroplast proteins. We then synthesized the uncharacterized peptides ARY, YLR, and LRM and measured the ACE inhibitory activity. LRM showed a low IC50 value (0.15 μmol) compared to ARY and YLR (1.3 and 5.8 μmol). In silico analysis revealed that the LRM sequence was conserved in cpcA from Bangiales and Florideophyceae, indicating that the novel ACE inhibitory peptide LRM was highly conserved in red algae.
• Sexual Reproduction via a 1-Aminocyclopropane-1-Carboxylic Acid-Dependent Pathway Through Redox Modulation in the Marine Red Alga Pyropia yezoensis (Rhodophyta).
  Toshiki Uji; Harune Endo; Hiroyuki Mizuta
  Frontiers in plant science, 11, 60, 60, 2020年, ［査読有り］, ［筆頭著者, 責任著者］, ［国際誌］
  英語, The transition from the vegetative to sexually reproductive phase is the most dynamic change to occur during a plant's life cycle. In the present study, we showed that the ethylene precursor 1-aminocylopropane-1-carboxylic acid (ACC) induces sexual reproduction in the marine red alga Pyropia yezoensis independently from ethylene. Exogenous application of ACC, which contains a three membered carbocyclic ring, promoted the formation of spermatia and carporspores in gametophytes, whereas ethephon, an ethylene-releasing compound, did not stimulate sexual reproduction. In addition, an ACC analog, 1-aminocyclobutane-1-carboxylic acid (ACBC), which contains a four membered carbocyclic ring, promoted sexual reproduction and enhanced tolerance to oxidative stress in the same manner as ACC, but 1-aminocyclopentane-1-carboxylic acid (cycloleucine; which contains a cyclopentane ring) did not. The application of ACC increased the generation of reactive oxygen species (ROS) and induced the expression of PyRboh gene encoding NADPH oxidase. ACC also stimulated the synthesis of ascorbate (AsA) by inducing transcripts of PyGalLDH, which encodes galactono-1,4-lactone dehydrogenase, the catalyst for the final enzymatic step of the AsA biosynthetic pathway. Conversely, ACC caused a decrease in the synthesis of glutathione (GSH) by repressing transcripts of PyGCL, which encodes glutamate cysteine ligase, the catalyst for the rate-limiting step in the formation of GSH. These results suggest a possible role played by ACC as a signaling molecule independent from ethylene in the regulation of sexual reproduction through alterations to the redox state in P. yezoensis.
• Transcriptomic analysis under ethylene precursor treatment uncovers the regulation of gene expression linked to sexual reproduction in the dioecious red alga Pyropia pseudolinearis
  Ryo Yanagisawa; Naoki Sekine; Hiroyuki Mizuta; Toshiki Uji
  JOURNAL OF APPLIED PHYCOLOGY, 31, 5, 3317, 3329, 2019年10月, ［査読有り］, ［責任著者］
  英語, 研究論文（学術雑誌）
• Complete sequence of mitochondrial DNA of Gloiopeltis furcata (Postels and Ruprecht) J. Agardh
  Watanabe Kei; Kishimoto Takahiro; Kumagai Yuya; Shimizu Takeshi; Uji Toshiki; Yasui Hajime; Kishimura Hideki
  MITOCHONDRIAL DNA PART B-RESOURCES, 4, 2, 2543, 2544, 2019年07月03日, ［査読有り］
• Characterization and expression profiles of small heat shock proteins in the marine red alga Pyropia yezoensis.
  Toshiki Uji; Yohei Gondaira; Satoru Fukuda; Hiroyuki Mizuta; Naotsune Saga
  Cell stress & chaperones, 24, 1, 223, 233, 2019年01月, ［査読有り］, ［筆頭著者, 責任著者］, ［国際誌］
  英語, 研究論文（学術雑誌）, Small heat shock proteins (sHSPs) are found in all three domains of life (Bacteria, Archaea, and Eukarya) and play a critical role in protecting organisms from a range of environmental stresses. However, little is known about their physiological functions in red algae. Therefore, we characterized the sHSPs (PysHSPs) in the red macroalga Pyropia yezoensis, which inhabits the upper intertidal zone where it experiences fluctuating stressful environmental conditions on a daily and seasonal basis, and examined their expression profiles at different developmental stages and under varying environmental conditions. We identified five PysHSPs (PysHSP18.8, 19.1, 19.2, 19.5, and 25.8). Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that expression of the genes PysHSP18.8, PysHSP19.5, and PysHSP25.8 was repressed at all the developmental stages under normal conditions, whereas PysHSP19.1 and PysHSP19.2 were overexpressed in mature gametophytes and sporophytes. Exposure of the gametophytes to high temperature, oxidative stress, or copper significantly increased the mRNA transcript levels of all the five genes, while exogenous application of the ethylene precursor 1-aminocylopropane-1-carboxylic acid (ACC) significantly increased the expression levels of PysHSP19.2, PysHSP19.5, and PysHSP25.8. These findings will help to further our understanding of the role of PysHSP genes and provide clues about how Pyropia species can adapt to the stressful conditions encountered in the upper intertidal zone during their life cycle.
• Control of elicitor-induced oxidative burst by abscisic acid associated with growth of Saccharina japonica (Phaeophyta, Laminariales) sporophytes
  Kazuki Shimizu; Toshiki Uji; Hajime Yasui; Hiroyuki Mizuta
  Journal of Applied Phycology, 30, 2, 1371, 1379, 2018年04月01日, ［査読有り］
  英語, 研究論文（学術雑誌）
• DISTAG/TBCCD1 is required for basal cell fate determination in Ectocarpus
  Olivier Godfroy; Toshiki Uji; Chikako Nagasato; Agnieszka P. Lipinska; Delphine Scornet; Akira F. Peters; Komlan Avia; Sebastien Colin; Laure Mignerot; Taizo Motomura; J. Mark Cock; Susana M. Coelho
  Plant Cell, 29, 12, 3102, 3122, 2017年12月01日, ［査読有り］
  英語, 研究論文（学術雑誌）
• Ethylene regulation of sexual reproduction in the marine red alga Pyropia yezoensis (Rhodophyta)
  Toshiki Uji; Ryuya Matsuda; Katsuaki Takechi; Hiroyoshi Takano; Hiroyuki Mizuta; Susumu Takio
  JOURNAL OF APPLIED PHYCOLOGY, 28, 6, 3501, 3509, 2016年12月, ［査読有り］, ［筆頭著者, 責任著者］
  英語, 研究論文（学術雑誌）
• Factors influencing the induction of adventitious bud and callus in the brown alga Sargassum horneri (Turner) C. Agardh
  Toshiki Uji; Daisuke Nanaumi; Chikara Kawagoe; Naotsune Saga; Kazuo Miyashita
  JOURNAL OF APPLIED PHYCOLOGY, 28, 4, 2435, 2443, 2016年08月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
  Hirotoshi Endo; Megumi Yoshida; Toshiki Uji; Naotsune Saga; Koji Inoue; Hiromichi Nagasawa
  SCIENTIFIC REPORTS, 6, 2016年03月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Characterization of an Eukaryotic PL-7 Alginate Lyase in the Marine Red Alga Pyropia yezoensis.
  Akira Inoue; Chieco Mashino; Toshiki Uji; Naotsune Saga; Koji Mikami; Takao Ojima
  Current biotechnology, 4, 3, 240, 248, 2015年08月, ［査読有り］, ［国際誌］
  英語, BACKGROUND: Alginate lyases belonging to polysaccharide lyase family-7 (PL-7) are the most well studied on their structures and functions among whole alginate lyases. However, all characterized PL-7 alginate lyases are from prokaryotic bacteria cells. Here we report the first identification of eukaryotic PL-7 alginate lyase from marine red alga Pyropia yezoensis. METHODS: The cDNA encoding an alginate lyase PyAly was cloned and was used for the construction of recombinant PyAly (rPyAly) expression system in Escherichia coli. Purified rPyAly was assayed to identify its enzymatic properties. Its expression pattern in P. yessoensis was also investigated. RESULTS: PyAly is likely a secreted protein consisting of an N-terminal signal peptide of 25 residues and a catalytic domain of 216 residues. The amino-acid sequence of the catalytic domain showed 19-29% identities to those of bacterial characterized alginate lyases classified into family PL-7. Recombinant PyAly protein, rPyAly, which was produced with E. coli BL21(DE3) by cold-inducible expression system, drastically decreased the viscosity of alginate solution in the early stage of reaction. The most preferable substrate for rPyAly was the poly(M) of alginate with an optimal temperature and pH at 35oC and 8.0, respectively. After reaction, unsaturated tri- and tetra-saccharides were produced from poly(M) as major end products. These enzymatic properties indicated that PyAly is an endolytic alginate lyase belonging to PL-7. Moreover, we found that the PyAly gene is split into 4 exons with 3 introns. PyAly was also specifically expressed in the gametophytic haplopid stage. CONCLUSION: This study demonstrates that PyAly in marine red alga P. yezoensis is a novel PL-7 alginate lyase with an endolytic manner. PyAly is a gametophyte-specifically expressed protein and its structural gene is composed of four exons and three introns. Thus, PyAly is the first enzymatically characterized eukaryotic PL-7 alginate lyase.
• 有用海藻における育種研究の現状と将来展望.
  宇治 利樹
  水産育種, 44:pp.41-48, 2015年
• DEVELOPMENT OF FORWARD GENETIC AND GENOMIC APPROACHES TO IDENTIFY KEY REGULATORY GENES IN THE BROWN ALGAE
  J. Mark Cock; Alok Arun; Delphine Scornet; Akira F. Peters; Olivier Godfroy; Alexandre Cormier; Nicolas Macaisne; Toshiki Uji; Sophia Ahmed; Susana M. Coelho
  EUROPEAN JOURNAL OF PHYCOLOGY, 50, 94, 94, 2015年, ［査読有り］
  英語
• Development of a nuclear transformation system with a codon-optimized selection marker and reporter genes in Pyropia yezoensis (Rhodophyta)
  Ryo Hirata; Toshiki Uji; Satoru Fukuda; Hiroyuki Mizuta; Asao Fujiyama; Satoshi Tabata; Naotsune Saga
  JOURNAL OF APPLIED PHYCOLOGY, 26, 4, 1863, 1868, 2014年08月, ［査読有り］
  英語, 研究論文（学術雑誌）
• A codon-optimized bacterial antibiotic gene used as selection marker for stable nuclear transformation in the marine red alga Pyropia yezoensis.
  Toshiki Uji; Ryo Hirata; Satoru Fukuda; Hiroyuki Mizuta; Naotsune Saga
  Marine biotechnology (New York, N.Y.), 16, 3, 251, 5, 2014年06月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）
• Changes in membrane fluidity and phospholipase D activity are required for heat activation of PyMBF1 in Pyropia yezoensis (Rhodophyta)
  Toshiki Uji; Rei Sato; Hiroyuki Mizuta; Naotsune Saga
  JOURNAL OF APPLIED PHYCOLOGY, 25, 6, 1887, 1893, 2013年12月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Characterization of the Sporophyte-Preferential Gene Promoter from the Red Alga Porphyra yezoensis Using Transient Gene Expression
  Toshiki Uji; Hiroyuki Mizuta; Naotsune Saga
  MARINE BIOTECHNOLOGY, 15, 2, 188, 196, 2013年04月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Molecular characterization and expression analysis of sodium pump genes in the marine red alga Porphyra yezoensis
  Toshiki Uji; Ryo Hirata; Koji Mikami; Hiroyuki Mizuta; Naotsune Saga
  MOLECULAR BIOLOGY REPORTS, 39, 8, 7973, 7980, 2012年08月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Transient gene expression systems in porphyra yezoensis: Establishment, application and limitation
  Koji Mikami; Toshiki Uji
  Porphyra yezoensis: Frontiers in Physiological and Molecular Biological Research, 93, 117, Nova Science Publishers, Inc., 2012年03月
  英語, 論文集(書籍)内論文
• Current status of "nori" cultivation and future on the application of genomic information
  Koji Mikami; Toshiki Uji; Ryo Hirata; Megumu Takahashi; Naotsune Saga
  NIPPON SUISAN GAKKAISHI, 78, 2, 271, 271, 2012年03月, ［査読有り］
  日本語, 研究論文（学術雑誌）
• ノリ育種の現状とゲノム情報利用の将来展望
  三上浩司; 平田遼; 宇治利樹; 髙橋潤; 嵯峨直恆
  水産育種, 41: pp. 161-168, 2012年
• A Dibasic Amino Acid Pair Conserved in the Activation Loop Directs Plasma Membrane Localization and Is Necessary for Activity of Plant Type I/II Phosphatidylinositol Phosphate Kinase
  Koji Mikami; Laura Saavedra; Yuji Hiwatashi; Toshiki Uji; Mitsuyasu Hasebe; Marianne Sommarin
  PLANT PHYSIOLOGY, 153, 3, 1004, 1015, 2010年07月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Visualization of Nuclear Localization of Transcription Factors with Cyan and Green Fluorescent Proteins in the Red Alga Porphyra yezoensis
  Toshiki Uji; Megumu Takahashi; Naotsune Saga; Koji Mikami
  MARINE BIOTECHNOLOGY, 12, 2, 150, 159, 2010年04月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis
  Megumu Takahashi; Toshiki Uji; Naotsune Saga; Koji Mikami
  Electronic Journal of Biotechnology, 13, 2, 1, 4, 2010年, ［査読有り］
  英語, 研究論文（学術雑誌）
• Visualization of Phosphoinositides via the Development of the Transient Expression System of a Cyan Fluorescent Protein in the Red Alga Porphyra yezoensis
  Koji Mikami; Toshiki Uji; Lin Li; Megumu Takahashi; Hajime Yasui; Naotsune Saga
  MARINE BIOTECHNOLOGY, 11, 5, 563, 569, 2009年10月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Structural features and gene-expression profiles of actin homologs in Porphyra yezoensis (Rhodophyta)
  Yukihiro Kitade; Michiko Nakamura; Toshiki Uji; Satoru Fukuda; Hirotoshi Endo; Naotsune Saga
  GENE, 423, 1, 79, 84, 2008年10月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis
  Satoru Fukuda; Koji Mikami; Toshiki Uji; Eun-Jeong Park; Toshiharu Ohba; Kiyozo Asada; Yukihiro Kitade; Hirotoshi Endo; Ikunoshin Kato; Naotsune Saga
  PLANT SCIENCE, 174, 3, 329, 339, 2008年03月, ［査読有り］
  英語, 研究論文（学術雑誌）

• 紅藻スサビノリの成熟誘導・色落ち条件におけるmicroRNAの働き
  小南彩夏; 佐々木駿; 水田浩之; 宇治利樹, 日本水産学会大会講演要旨集(CD-ROM), 2024, 2024年
• 海苔の原料であるスサビノリにおけるエチレン前駆物質の役割について
  宇治 利樹, アグリバイオ, 2022年10月, ［筆頭著者］
• Identification, Characterization, and Expression Analysis of Spondin-Like and Fasciclin-Like Genes in Neopyropia yezoensis, A Marine Red Alga
  Toshiki Uji; Shinnosuke Ueda; Hiroyuki Mizuta, Phycology, 2, 1, 45, 59, 2022年01月06日
  Extracellular matrix (ECM) proteins play crucial roles in the regulation of cell proliferation and differentiation. We identified homologous genes encoding ECM proteins that are known to associate with integrins in animal cells in red macroalga Neopyropia yezoensis. Four genes encoding spondin domain-containing proteins (NySPLs) and eight genes encoding fasciclin domain-containing proteins (NyFALs) from N. yezoensis were selected for bioinformatics and expression analysis in order to obtain insights into the roles of ECM proteins for the life cycle. NySPLs had eight β-strands with two contiguous α-helices, which were similar to those of the F-spondin domain of animals. NyFALs had conserved H1 and H2 motifs and a YH motif between the H1 and H2 regions. Quantitative reverse transcription polymerase chain reaction showed that NySPL1–3 and NyFAL8 transcripts were highly accumulated in mature gametophytes that formed the spermatia. Furthermore, expressions of all NySPLs were upregulated in response to the ethylene precursor 1-aminocylopropane-1-carboxylic acid that induces gametogenesis. NyFAL1, 4 were highly expressed in sporophytes, whereas NyFAL2, 3, 5, 6, and 7 were overexpressed in gametophytes, especially at the vegetative stage. These findings facilitate future research on ECM architecture in the unique life cycles of red macroalgae., MDPI AG
• 紅藻スサビノリの日周性におけるトランスクリプトーム解析
  小南彩夏; 水田浩之; 宇治利樹, 日本水産学会大会講演要旨集(CD-ROM), 2021, 2021年
• 紅藻類の有性生殖に関わる制御機構-アマノリ類における1-アミノシクロプロパンカルボン酸の役割を中心に-
  宇治利樹, 藻類, 69, 1, 2021年, ［査読有り］, ［招待有り］
• 海洋深層水を活用した海藻スプラウトの陸上養殖と利用適性に関する研究
  木下康宣; 木村和世; 川越力; 高野智宏; 熊谷祐也; 岸村栄毅; 宇治利樹; 水田浩之, 北海道立工業技術センター研究成果発表会要旨集(Web), 2021, 2021年
• 東北地方沖太平洋の流れ藻から採集された甲殻類および魚類
  樋口淳也; 河合俊郎; 宇治利樹; 今井圭理, 北海道大学水産科学研究彙報(Web), 71, 1, 2021年
• スサビノリの野生株と外来遺伝子導入株の交雑
  泉ひかり; 宇治利樹; 水田浩之; 嵯峨直恆; 福田覚, 日本水産学会大会講演要旨集, 2020, 2020年
• グリシンによるスサビノリの有性生殖の促進効果
  小野寺翔太; 水田浩之; 宇治利樹, 日本水産学会大会講演要旨集, 2019, 2019年
• スサビノリの有性生殖は,エチレンではなく,その前駆物質によって制御される
  遠藤晴音; 水田浩之; 宇治利樹, 日本水産学会大会講演要旨集, 2019, 2019年
• スサビノリ葉状体におけるブロモペルオキシダーゼ遺伝子の発現機構解析
  松田竜也; 宇治利樹; 武智克彰; 高野博嘉; 瀧尾進, マリンバイオテクノロジー学会大会講演要旨集, 17th, 114, 2015年05月30日
  日本語
• 紅藻スサビノリにおける改変ハイグロマイシン耐性遺伝子を選択マーカーとした形質転換技術の開発
  宇治利樹; 平田遼; 福田覚; 水田浩之; 嵯峨直恆, 日本水産学会大会講演要旨集, 2014, 2014年
• Molecular characterization and expression analysis of two Na+/H+ antiporter genes in the marine red alga Porphyra yezoensis
  Toshiki Uji; Ryota Monma; Hiroyuki Mizuta; Naotsune Saga, FISHERIES SCIENCE, 78, 5, 985, 991, 2012年09月
  英語
• Development of an expression system using the heat shock protein 70 promoter in the red macroalga, Porphyra tenera
  Su Hyun Son; Joon-Woo Ahn; Toshiki Uji; Dong-Woog Choi; Eun-Jeong Park; Mi Sook Hwang; Jang Ryol Liu; Dongsu Choi; Koji Mikami; Won-Joong Jeong, JOURNAL OF APPLIED PHYCOLOGY, 24, 1, 79, 87, 2012年01月
  英語
• 海産紅藻スサビノリの異型世代交代における胞子体世代優占的な遺伝子転写制御機構の解析
  宇治利樹; 嵯峨直恆; 三上浩司, 日本植物生理学会年会要旨集, 52nd, 206, 2011年03月11日
  日本語
• 海産原始紅藻スサビノリPorphyra yezoensisのプロトプラストへの蛍光タンパク質遺伝子導入法の開発
  橋潤; 宇治利樹; 嵯峨直恆; 三上浩司, 日本植物生理学会年会要旨集, 50th, 372, 2009年03月16日
  日本語
• 紅藻スサビノリにおける一過的遺伝子発現系の開発
  宇治利樹; 三上浩司; 福田覚; 大場利治; 浅田起代蔵; 北出幸広; 遠藤博寿; 朴恩貞; 加藤郁之進; 嵯峨直恆, 日本植物生理学会年会要旨集, 49th, 2008年
• 紅藻スサビノリ配偶体の成熟をモニターする分子マーカーの探索
  北出幸広; 中村理湖; 宇治利樹; 福田覚; 遠藤博寿; 嵯峨直恆, 生化学, 2007年

• Emergence of Ectocarpus as a model system to study the evolution of complex multicellularity in the brown algae. In: "Evolutionary transitions to multicellular life"
  Cock J.M; Godfroy O; Strittmatter M; Scornet D; Uji T; Farnham G; Peters A.F. Coelho S
  Springer, pp 153-162, (2015)
• Transient gene expression systems in Porphyra yezoensis: establishment, application and limitation.Porphyra yezoensis: Frontiers in Physiological and Molecular Biological Research.
  Mikami K; Uji T
  New York: Nova Science Publishers, pp 93-117. (2012)
• Transient transformation of red algal cells: Breakthrough toward genetic transformation of marine crop Porphyra species.
  Mikami K; Hirata R; Takahashi M; Uji T; Saga N
  InTech-Open Access Publisher, pp 241-258. (2011)

• 育種生物学特論Ⅱ, 2024年, 修士課程, 水産科学院
• 水圏生物学実験, 2024年, 学士課程, 水産学部
• 教科教育法（理科Ⅱ）, 2024年, 学士課程, 教育学部
• 水産増養殖実習, 2024年, 学士課程, 水産学部
• 水族遺伝育種学, 2024年, 学士課程, 水産学部
• 基礎生命科学実験, 2024年, 学士課程, 水産学部
• 発生・組織学, 2024年, 学士課程, 水産学部

• マリンバイオテクノロジー学会
• 日本水産学会

• 有用紅藻類における生殖機構に関する研究
  LASBOS Moodle, 宇治 利樹, 2020年04月28日 - 現在, ［教材］
• 海藻における有用遺伝子の探索手法の開発
  2022年05月16日, ［教材］

• 紅藻スサビノリにおけるエチレン前駆物質による炭素・窒素代謝バランスの制御
  科学研究費助成事業 基盤研究(C)
  2022年04月01日 - 2025年03月31日
  宇治 利樹
  日本学術振興会, 基盤研究(C), 北海道大学, 22K05779
• エチレン前駆物質による紅藻スサビノリの有性生殖の制御機構に関する研究
  科学研究費助成事業 若手研究
  2019年04月01日 - 2022年03月31日
  宇治 利樹
  日本学術振興会, 若手研究, 北海道大学, 19K15907
• アミノ酸添加による有用海藻類の健全かつ効率的な種苗生産技術の開発
  令和2年度研究助成（個人研究の部）
  2020年09月 - 2021年09月
  栗林育英学術財団, 研究代表者
• 岩海苔の安定的な増養殖を目指した胞子形成および放出機構の解明
  若手研究人材・ネットワーク育成補助金
  2020年09月 - 2021年03月
  ノーステック財団, 研究代表者
• 北方系海藻の通年収穫を目的とした陸上栽培技術の開発
  2019年08月 - 2020年03月
  川越 力; 水田浩之; 宇治利樹; 木下康宣; 岸村栄毅; 熊谷祐也
  ノーステック財団 令和元年度（2019年度）「研究開発助成事業」 スタートアップ研究補助金
• 海苔の有用品種作出に向けたスサビノリのエチレン応答機構の解明
  科学研究費助成事業 若手研究(B)
  2016年04月01日 - 2019年03月31日
  宇治 利樹
  本研究では、海苔の原料であるスサビノリの有性生殖や酸化ストレス応答にエチレンではなく、その前駆物質である1-アミノシクロプロパン-1-カルボン酸(ACC)が関与していることを明らかにし、海藻において初めて、ACC依存的なシグナル伝達系の存在の可能性を示した。またその応答にはNADPHオキシダーゼ由来の活性酸素種（ROS）が関与していることも見出した。さらにACC応答性遺伝子として、小胞体輸送や細胞分裂、抗酸化に関連する遺伝子を同定し、これらの遺伝子が本種の有性生殖や抗酸化獲得に関与している可能性が明らかとなった。
  日本学術振興会, 若手研究(B), 北海道大学, 研究代表者, 競争的資金, 16K18740
• 「次世代型アカモク養殖を目指したビックデータ構築と種苗生産の確立
  COI拠点「食と健康の達人」拠点 」
  2018年12月 - 2019年03月
  宇治 利樹
  研究代表者, 競争的資金
• 海苔の有用品種作出に向けた紅藻スサビノリにおける高温応答機構の解明
  科学研究費助成事業 特別研究員奨励費
  2015年04月24日 - 2016年03月31日
  宇治 利樹
  本研究では当初、エチレン（ACC）と高温耐性の関連に焦点を当てて解析を行ったが、高温耐性以外の効果について以下のことが明らかとなった。スサビノリ由来のACC合成酵素遺伝子PyACSが銅およびH2O2ストレス条件下において発現が誘導されることから、エチレンがこれらのストレスに対して応答するために重要な機能をしている可能性が示唆された。そこで、ACC処理による銅ストレスやH2O2ストレス耐性の効果を調べたところ、未処理の藻体と比較して、ACC処理藻体では大量の果胞子様細胞を形成することにより、これらのストレスに適応することが明らかとなった。また長期間（２週間以上）のACC処理により、光合成色素タンパク質であるフィコエリスリン、フィコシアニンおよびクロロフィルの分解が促進されること、生殖細胞の形成が促進されることが明らかとなった。そのため、エチレンの応答機構を解明することにより、高温耐性等の環境ストレス応答機構に加えて、色落ちや有性成熟の機構の解明につながることが期待される。そしてこれらの知見は海苔の品種改良を進めていく上で重要な知見になることが予想される。
  
  
  またエチレンのスサビノリに対する作用機序に関する知見を得るために、エチレンによって制御されている遺伝子群の網羅的な同定を試みた。次世代シーケンサー（イルミナ社Hiseq2000）を用いてRNA-seq解析を行った結果、ストレス耐性に関与する候補遺伝子として、カタラーゼ、分子シャペロン（DnaJ）およびプロテインキナーゼ遺伝子等の発現が、ACC処理藻体サンプルにおいて上昇することが明らかとなった。またエチレン応答性遺伝子として、RWP-RKドメインを持つ遺伝子を見出した。この遺伝子は他の生物において転写調節因子をコードすることから、スサビノリにおいても重要な機能を持つ可能性が考えられた。
  日本学術振興会, 特別研究員奨励費, 北海道大学, 研究代表者, 競争的資金, 15J05434
• 褐藻シオミドロの性分化と成熟機構の解明
  平成23年度海外留学助成ポストドクトラルフェローシップ
  2011年 - 2012年
  宇治 利樹
  上原記念生命科学財団, 研究代表者, 競争的資金
• 多細胞真核生物における世代交代の多様化を制御する分子機構の研究
  平成21年度博士後期課程在学生研究助成
  2009年 - 2010年
  宇治 利樹
  北海道大学クラーク記念財団, 研究代表者, 競争的資金
• 雌雄異株アマノリ属を用いた海産紅藻類における性決定機構の解明
  2017年度研究助成奨励
  宇治 利樹
  秋山生命科学振興財団, 研究代表者, 競争的資金